LOCUS       DAUELZ010000001        49299 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.001, whole genome shotgun sequence.
ACCESSION   DAUELZ010000001 DAUELZ010000000
VERSION     DAUELZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49299
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.001"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            49..1347
                     /gene="rocA"
                     /locus_tag="VHI09_000001"
     CDS             49..1347
                     /gene="rocA"
                     /locus_tag="VHI09_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEQ6119918.1"
                     /translation="MLTLKLFSFVSAIPLRLKNIFYLSLSMVLFQVVFWAFFPDHFIL
                     DVVMLAQFLFFALIALYYGKSIKAKFLMFYAFFPLVSISLVKRFIVFFVMPLFGMPYS
                     VVKHNTLLIYSITCFSIFLIYRCIQVFHFDFSTWRQYFQSHRASKLLVFTNSSMALYY
                     LCIQGIDVMSPSLSGLATTTARSIIVLFYFILFLTLLIHLERYVKQNSIEAIVQQKEY
                     RELINYSQHLGLLYQDIQELRQLLTTVSSRLKIGIEQNDISIVRLTYEGILNAEKNNA
                     KDDRLDLTCLDKLQVEAIRHIVLAKLIEAKNKKLKVEVSIPNCIATFFLEVVDFTKLL
                     SFLLDNAIEMSLETKQPCLSIAFLDQNHKLVIVIQSSTKQGQNDSQSVFATPALKKRD
                     DWQFDLRNVTTILNRYDYLTISSQIHDGILTQLIEIAKPD"
     gene            complement(1448..4153)
                     /locus_tag="VHI09_000002"
     CDS             complement(1448..4153)
                     /locus_tag="VHI09_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEQ6119919.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTDP
                     DFHSFHLDGQTIILDDYLQVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDRWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDNSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLSDLANDLPDNL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLRYAWKTLTQNHPHDSICGCSVDSVHREMMNRFEKAYEVGHYLAKEAAQQIAGAV
                     NTSDFPTDSQPFLLFNTSGHSKTSVTELTLTWKKYYFGQRFPKEVYQEAQEYLAGLSQ
                     SFQVIDVSGNTISEADILDTSIAFDYDLPKRTFREPYFAIKVRLRLPITLPAMSWKAL
                     ALQLGNETAPSATVPLYDDSNQCLENEFLKVMIQTDGRLTITDKQSGVSYQDLLRFED
                     CGDIGNEYISRQPTHDQPFYADQGTTKLNIISNTAQVAELEIQQTFAIPISADKLLQA
                     EMEAVIDITERQARRSQEKAELTLTTLIRMEKNNPRLQFTTSFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPDATFWKNPSNPQHQECFVSLFDGENGVTIGNYGLNE
                     YEILPDTNTIAITLLRSVGEMGDWGYFPTPEAQCLGKHSLSYSFESITKQTQFASYWR
                     AQEGQVPILVEQTSHHKGSLRAEHSYVTGADDQVALTAFKRRPSDNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(4168..5454)
                     /locus_tag="VHI09_000003"
     CDS             complement(4168..5454)
                     /locus_tag="VHI09_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEQ6119920.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQIKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANGKGHQTDRTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHKGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNESVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTILSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSITP"
     gene            5587..6627
                     /locus_tag="VHI09_000004"
     CDS             5587..6627
                     /locus_tag="VHI09_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ6119921.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAKKQVTSPEANKDLLLILPFASDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LSIPDQVAIVGFDNSQASRLLSPKLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            6678..8372
                     /locus_tag="VHI09_000005"
     CDS             6678..8372
                     /locus_tag="VHI09_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEQ6119922.1"
                     /translation="MTIYHTLKDYQEVMTRGDYLVFDTPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAEVTMKHFFNRQLAKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFAMRGIIEGFYGTPWTREERLDCLRFIGNKRM
                     NTYMYAPKDDDYQRKQWRDLYPEDWVTYFKELLAVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAIYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQPDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSHSWLETLTDYTEDTELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKNVSTLSAWVDELVELVNTLRKLDKPAFQQAIAPWFERVAKDQDFWQAM
                     LNQEPQLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            8397..9839
                     /locus_tag="VHI09_000006"
     CDS             8397..9839
                     /locus_tag="VHI09_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ6119923.1"
                     /translation="MLAFPKDFWWGGATSGPQSEGRFAKQHRNLFDYWYEQEPTLFYD
                     YVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQVYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGR
                     IGTILNLTPAYPASQSEADTAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPEWYYDAYLMPGRRMN
                     VDKGWEIYPEAVYDIAVKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIRF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNCLISLKVMEEFGGSASHLSD"
     gene            complement(10450..11328)
                     /locus_tag="VHI09_000007"
     CDS             complement(10450..11328)
                     /locus_tag="VHI09_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ6119924.1"
                     /translation="MSLLCLDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAVSAPGAVNKEKGIIEGASAIPYIHHFKIQEALEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVLGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALAEQGDHLAIKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            11545..12495
                     /locus_tag="VHI09_000008"
     CDS             11545..12495
                     /locus_tag="VHI09_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ6119925.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIAFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLTGSG
                     GAPIKWYSNPTWWPLILLFMSVWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDASLF"
     gene            12505..13431
                     /locus_tag="VHI09_000009"
     CDS             12505..13431
                     /locus_tag="VHI09_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ6119926.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            13512..14957
                     /locus_tag="VHI09_000010"
     CDS             13512..14957
                     /locus_tag="VHI09_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ6119927.1"
                     /translation="MKKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKNQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            15263..15868
                     /locus_tag="VHI09_000011"
     CDS             15263..15868
                     /locus_tag="VHI09_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEQ6119928.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTPQEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTMTYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     TLMTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            15858..17582
                     /locus_tag="VHI09_000012"
     CDS             15858..17582
                     /locus_tag="VHI09_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ6119929.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLSAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQSQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADRVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRHDNALSIKALDETDHFLIQVLDN
                     GRGISQERLADMERRLQEHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDA"
     gene            17582..19066
                     /locus_tag="VHI09_000013"
     CDS             17582..19066
                     /locus_tag="VHI09_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ6119930.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDCLDAQQAESIRQEAYHDSLLTLWLTDELSEKEFQQLSQG
                     LPAAALTGFTVLYLDCQEWQAAIDAYFEREGQPFYLKKEEDKKVYLAVLLGKTDQAKA
                     FTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSLFYNCEVSPIRAKDKSKIEL
                     PESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTLNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            18996..22502
                     /locus_tag="VHI09_000014"
     CDS             18996..22502
                     /locus_tag="VHI09_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEQ6119931.1"
                     /translation="MVYRQKSSEIVTRTITKLSRVTSNVKQEIDMTSDSKEQTVTGYQ
                     YHYIDQEGRKQPFNQGWRFLMADVACAQDPSFDDSNWQVIHLPHDFSLTQPYTRNGEA
                     ESAYKLGGVGWYRHYLVLDEVLAGYHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFALNQSIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFMANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENANYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATFLSQLDHPQGLIGLNYANGKDYDKLHEEHSDWLLYGSET
                     ASAITSRAYYKEPKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNLTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSRGELVSHAQDWLQVQVEGPARLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEEGHIKVTAKVSQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKVSTPHILITSYQHY
                     LSLPLQVCSQAFDFKKGDDIFLPQWLQVKDAQGSLVKKAFSMTWYLKEKIFAKPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLTASQKRLTYCYQYDTVQAIGA
                     ISLQNHTDTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLSVT
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            22640..23518
                     /locus_tag="VHI09_000015"
     CDS             22640..23518
                     /locus_tag="VHI09_000015"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ6119932.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEVRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            23770..24525
                     /locus_tag="VHI09_000016"
     CDS             23770..24525
                     /locus_tag="VHI09_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEQ6119933.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVAVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAQAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            24578..24916
                     /locus_tag="VHI09_000017"
     CDS             24578..24916
                     /locus_tag="VHI09_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ6119934.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAGIPHALYAVEAFQ
                     MLLVVVKPEA"
     gene            24918..25223
                     /locus_tag="VHI09_000018"
     CDS             24918..25223
                     /locus_tag="VHI09_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ6119935.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPRDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKVHHLEAKTDCKLLQVLLD"
     gene            complement(25659..26732)
                     /locus_tag="VHI09_000019"
     CDS             complement(25659..26732)
                     /locus_tag="VHI09_000019"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEQ6119936.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKSLTNMIGSIYPPE
                     AVIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKRIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(26788..27540)
                     /locus_tag="VHI09_000020"
     CDS             complement(26788..27540)
                     /locus_tag="VHI09_000020"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEQ6119937.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            complement(27782..27852)
                     /locus_tag="VHI09_000021"
     tRNA            complement(27782..27852)
                     /locus_tag="VHI09_000021"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27818..27820),aa:Thr,seq:ggt)
     gene            28019..28273
                     /locus_tag="VHI09_000022"
     CDS             28019..28273
                     /locus_tag="VHI09_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEQ6119938.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            28294..28689
                     /locus_tag="VHI09_000023"
     CDS             28294..28689
                     /locus_tag="VHI09_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEQ6119939.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            complement(28712..28945)
                     /locus_tag="VHI09_000024"
     CDS             complement(28712..28945)
                     /locus_tag="VHI09_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6119940.1"
                     /translation="MKPNNPYPAYTGQGRISCYDFRGTTTVSDHKMGLLLFISDFLSS
                     ATSLDICADFSTPPLSVTDQLNAPLIANILTNL"
     gene            28975..29294
                     /locus_tag="VHI09_000025"
                     /pseudo
     CDS             28975..29294
                     /locus_tag="VHI09_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29291..29854
                     /locus_tag="VHI09_000026"
     CDS             29291..29854
                     /locus_tag="VHI09_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEQ6119941.1"
                     /translation="MNLIIIGAQASGKMTIGQEVARQTGMTLFHNHDSIDFVLRFMPW
                     SQESTALIERIRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLTRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTHLTAAETAQLIIQKMTHIKEN"
     gene            29856..32504
                     /locus_tag="VHI09_000027"
     CDS             29856..32504
                     /locus_tag="VHI09_000027"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEQ6119942.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGQGI
                     TRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKHVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAVVEPRLSTQWFVKMDELAKQAMDNQETDDRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEDAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIV
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVERLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLGNERFVANAKPEVIQKE
                     KDKQADYQAKYDATQERIAEMQKIKS"
     gene            32658..34657
                     /locus_tag="VHI09_000028"
                     /pseudo
     CDS             32658..34657
                     /locus_tag="VHI09_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            34790..35518
                     /gene="cas5c"
                     /locus_tag="VHI09_000029"
     CDS             34790..35518
                     /gene="cas5c"
                     /locus_tag="VHI09_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEQ6119943.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPASKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            35518..37413
                     /gene="cas8c"
                     /locus_tag="VHI09_000030"
     CDS             35518..37413
                     /gene="cas8c"
                     /locus_tag="VHI09_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEQ6119944.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRSNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIAPDYQHNQLK
                     VIYFDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKALHQSYISFVEANRENLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLMI
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            37418..38266
                     /gene="cas7c"
                     /locus_tag="VHI09_000031"
     CDS             37418..38266
                     /gene="cas7c"
                     /locus_tag="VHI09_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEQ6119945.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLERRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLEGSINAYFAEKTGFSQEDAEAIKEVLVSLCENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYHQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     EILEGL"
     gene            38268..38942
                     /gene="cas4"
                     /locus_tag="VHI09_000032"
     CDS             38268..38942
                     /gene="cas4"
                     /locus_tag="VHI09_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEQ6119946.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMQEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            38939..39964
                     /gene="cas1c"
                     /locus_tag="VHI09_000033"
     CDS             38939..39964
                     /gene="cas1c"
                     /locus_tag="VHI09_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ6119947.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGCASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILMTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            39975..40268
                     /gene="cas2"
                     /locus_tag="VHI09_000034"
     CDS             39975..40268
                     /gene="cas2"
                     /locus_tag="VHI09_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ6119948.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     repeat_region   40417..40777
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=40417..40448
                     /rpt_unit_seq="gtctcacccttcatgggtgagtggattgaaat"
     gene            40937..41647
                     /gene="ccdA2"
                     /locus_tag="VHI09_000035"
     CDS             40937..41647
                     /gene="ccdA2"
                     /locus_tag="VHI09_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEQ6119949.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFDKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHMLLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            41660..42283
                     /locus_tag="VHI09_000036"
     CDS             41660..42283
                     /locus_tag="VHI09_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEQ6119950.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMAKKEITQDKMSMAAKKKD
                     EMSTSKDKSMMADKTSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFTKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            42326..43432
                     /gene="msrB"
                     /locus_tag="VHI09_000037"
     CDS             42326..43432
                     /gene="msrB"
                     /locus_tag="VHI09_000037"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ6119951.1"
                     /translation="MEHKWKLWLFIVGVVLLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKVDPNAPVDQKMIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVAYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEEYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPLSPDVVRYKEDKSFNMTRTEVRSRSGNSHLGHAFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            43520..44260
                     /locus_tag="VHI09_000038"
     CDS             43520..44260
                     /locus_tag="VHI09_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ6119952.1"
                     /translation="MYSLLIVEDEYLIRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKEHAPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLRKKLDLSKKTETIQELVEQPQKEVSAIAMAIHEQLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKELGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
     gene            44257..45990
                     /locus_tag="VHI09_000039"
     CDS             44257..45990
                     /locus_tag="VHI09_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ6119953.1"
                     /translation="MKRYPLLVQLISYVFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETISVLAKNSEIKDFASQIHQENDKQVFQLMKMVLATN
                     SDLQAAVLVTKDGRTVSTNPQLIMKTSSDMMAEPWYKAAIDRQAMPILTPARQLSLSS
                     KKEWVVSVTQEVVDAVGHNLGVLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRV
                     QRQIFWSFLGTGLLACLICSFATVLALRRWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTDLVQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYE
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLMLTVWDNGK
                     GIEDSSLTNSQSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLSLPKMH
                     ELMADDTQENE"
     gene            46063..47142
                     /locus_tag="VHI09_000040"
     CDS             46063..47142
                     /locus_tag="VHI09_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEQ6119954.1"
                     /translation="MSLASDYFSRQTPIVEKLMAYGFEKRNNGYFYNERFMEEEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEATP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVGGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKDLPQLLQQEGIYPAYHMSKKNWISIILDDKITDDKLWTLVTQ
                     SRQLVNPNGLSNPNGPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYVCQVLETDIPNEGYRKNPNIDKLMRLRKCQQYKDGLLSFDLMKEHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            47156..47872
                     /locus_tag="VHI09_000041"
     CDS             47156..47872
                     /locus_tag="VHI09_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEQ6119955.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPAGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNSTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALKFIEGSAKMYLHA
                     ITSVHVLDKDNPHVPLFTEN"
     gene            complement(48039..48512)
                     /locus_tag="VHI09_000042"
     CDS             complement(48039..48512)
                     /locus_tag="VHI09_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ6119956.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATICGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            48654..>49299
                     /locus_tag="VHI09_000043"
     CDS             48654..>49299
                     /locus_tag="VHI09_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ6119957.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTY"
BASE COUNT        14780 a         9189 c        10704 g        14626 t
ORIGIN      
        1 gattttcttc aatttttagg atttatattt ttagatatta ttgaaattat gttaacgtta
       61 aagcttttta gctttgtaag tgcaattccg cttcgcctga aaaatatttt ttatttatca
      121 cttagtatgg ttttgtttca agtagttttt tgggctttct ttccagacca tttcatcctt
      181 gatgttgtga tgttggctca gtttcttttc tttgcgctta tagccctata ctatggtaaa
      241 tcaataaaag ctaagttttt aatgttttat gccttttttc cactagtgtc tattagtttg
      301 gttaagcgat ttattgtatt ttttgttatg ccattgtttg gtatgcctta ttcagttgtt
      361 aaacataata cactactgat ttacagcatt acttgtttta gtattttttt gatttatcgc
      421 tgcattcaag tttttcattt cgatttttca acctggcgcc aatattttca atcacataga
      481 gctagtaaac tattagtgtt tactaactct tcgatggctc tatattactt gtgtatccaa
      541 gggatagatg tgatgtcgcc ttctttatca ggacttgcta caacgactgc tcgttcaatc
      601 atagtgctct tttatttcat tttgtttctt actttattaa ttcatttaga gcgttatgta
      661 aaacaaaact ctattgaggc aattgtgcaa caaaaagaat atcgtgagct gattaattat
      721 agtcagcacc ttggattgct gtatcaagat attcaagagc ttaggcagct attaactacc
      781 gtgtccagtc gtcttaagat tggaatcgaa caaaatgata tctctattgt gagacttact
      841 tatgaaggta tcttgaatgc tgaaaagaat aatgctaaag atgacagact tgatttaact
      901 tgtttagata aattacaagt tgaagcaatc agacatattg ttttagctaa attaattgag
      961 gcaaaaaata agaagcttaa ggttgaggta tcgatcccta attgtattgc aacgtttttt
     1021 ctagaagtag tagatttcac taagctattg tcatttttgt tagataatgc tatagaaatg
     1081 agtttggaga caaagcaacc ttgcttatca atagcatttc tggatcaaaa ccataaactt
     1141 gtcatagtca ttcaaagcag tactaaacaa ggacaaaatg atagtcaaag cgtgtttgct
     1201 acaccggctt tgaaaaaaag agatgactgg caatttgact taaggaatgt cacgaccatt
     1261 ttaaatcgtt atgactatct cacaattagc tcgcagattc atgatggcat tttaacccag
     1321 ttaatagaaa tagctaagcc tgactgatgt taaaggtatt aataatcact taaaatcaac
     1381 ttaagagtct gagacaatag tcccagactc tttttgaata tgttaatcta ttactaatga
     1441 taatagttta ttgtttcttc caagctagag ttaaaatttc agctttgcca agttggctgg
     1501 gtgtgctttg cttgctgtct ttttctaaca aattagtgac cttagcattg tagtttggca
     1561 ggcttaggct aaagtcacaa gttttatcgt ttgagagatt gtagctgcgc gtgataaggg
     1621 cattgtctga tgggcgacgt ttgaaagccg ttagagcgac ttgatcgtct gccccagtga
     1681 catagctatg ctctgctctt aagcttccct tatgatggct ggtttgttcg actaggatag
     1741 gaacttggcc ttcttgtgcc cgccaatagc tggcaaattg tgtttgctta gtgatgcttt
     1801 caaaactata agataggctg tgtttgccga ggcattgggc ttcaggagta gggaaatacc
     1861 cccagtcgcc catttcacca acagaacgta agagagtgat ggcaatggtg ttggtatctg
     1921 gtaagatctc atattcgttg aggccatagt taccaatagt gactccattt tcaccatcaa
     1981 agagactcac aaagcattct tggtgctgtg ggttacttgg attcttccaa aaggtggcat
     2041 ctggatgatt tggacgtttg acagtttcaa aaatactgtc agctagatga tggtctgttt
     2101 taaggtgcgt tgggaatagg acgcgcaagc gatgattagt catttggtta tcaaaacttg
     2161 tggtgaattg gaggcgagga ttgtttttct ccatgcggat aagggttgtt aaggttagct
     2221 cagccttttc ttgtgaacgt cttgcttggc gttctgtgat gtcaatgaca gcttccatct
     2281 cagcctgtaa gagcttatct gcggagatag gaatggcaaa ggtttgctgg atttcaagtt
     2341 cagcaacttg agcggtgttg ctaatgatgt taagcttggt ggtcccttgg tccgcataga
     2401 aaggttggtc atgagttggt tggcgggaaa tgtattcatt tccaatatcg ccacaatctt
     2461 caaaccgtag gagatcttga tagctcaccc ctgactgttt atcggtgatg gttagacggc
     2521 catcggtttg tatcataact tttagaaact cattttcaag gcactgatta ctatcgtcgt
     2581 agagtgggac cgttgctgaa ggagctgtct cgtttcctag ttgtaatgct aaggctttcc
     2641 aagacatggc agggagagtt attggtagcc gtaatctcac tttgatggcg aaataaggtt
     2701 ctcggaaggt tctcttgggc aaatcgtagt caaaggcgat gcttgtgtcc aaaatgtctg
     2761 cttctgagat agtgtttcct gatacatcaa tgacttggaa agattgggag agtcctgcca
     2821 aatactcttg agcttcttgg taaacctctt taggaaagcg ttggccaaaa taatattttt
     2881 tccaggtcag ggtgagctca gtaacacttg ttttggaatg gccactggta ttaaataaga
     2941 ggaaaggttg gctatctgtt ggaaagtcac tcgtgttaac tgcgccagca atttgctggg
     3001 cagcttcttt tgctaaatag tgtccgactt cataggcttt ttcaaaacgg ttcatcattt
     3061 cccgatgaac actatcaaca ctacaaccac agatagaatc atgagggtga ttttgcgtga
     3121 gggttttcca agcgtaacgc agttggtcgt gagggtaggt acttgtcacc tcataagcca
     3181 ttgctgctaa gggttcggtg atgttttcga gttgacgaga gactctggta ttagcctgtt
     3241 tgaggtaaat gcgagcagaa gccgtgttag ctagggtata ccagccatct gtctcttgac
     3301 tggtaatctc tccttggacg gttgaaaggt tatctggtaa atcatttgcg agatcagaca
     3361 agtaatcttc aaagcaggaa tgcacaaatt cgtaatcagg atagagttgg ttggctaagg
     3421 cgattgcctt ggtgacatca agttgtacgg gttgatgatc acacccgttc atcatcagaa
     3481 ggtgcttggt tgaggcaaag cgttcagcgt cagcaagttt tttatcccaa aaaagacgcg
     3541 cctcagcttc tgttgtcggg atctcattgc cattgctgta ccagttggcg aagaggagtc
     3601 caagaatacg actgttatct gggccttgcc aactgatttc agagaattgt gagctgtatt
     3661 tttcactggt atctacttga ttgttaaatc cagttggacg aatgccacga ccaaaggcag
     3721 cagcttgtag gccggctttt aacatcagct gtggtgtttg tcccatattt ccaaaggtat
     3781 caggaaaata accaagtggc acactagcgc cccatctgtc acaatcttcc ttaccaatca
     3841 gcatattgcg cacattggat tcactgctgg tcaaaaaatc gtcctgtaag atatagaaag
     3901 gtccgatacg gagttttccc gaagcaatgg cttgtctaat ctcaggttct cgttcggggc
     3961 gtacttgcaa ataatcatct aggataatgg tttgcccatc caaatgaaaa ctatgaaaat
     4021 caggatccgt ttgaaaaact tctaacaggt catctattaa gttaatcaga cgcatgtggt
     4081 gttgttcgta agccatgtac cactcgcgat cccagtgact gtgtgaaata atatgtactt
     4141 ttttagttgc catagtggcc tcactttcta aggagtaatg ctaaagccaa gatagtcaag
     4201 aagtagttca caaaacatca tgttagccca agagaaccac tcacgagtgt attgataagg
     4261 atcattgaca tcgaaaccct cgtgcattaa gtgggtacca gcatctgttg ccacaagcaa
     4321 atcaagcatc tcttttttac tgtcttggtc aagagcggtc aatccttgta aggcaagggc
     4381 gatatgccag atataatttt ctggtgtatg ggaagaaccg atgcctgctg ctgcatttcc
     4441 ctgataataa taagggtttt cttggctgag aatggtgcgg cgcgttgcca aatagatagg
     4501 gtcatcttta gcgcaaaaac caaggtaagg tgcagcaagg aggcttggaa tattagcatc
     4561 atccatgatg gaaaactgtc ccagaccatc cacttcataa gcgtaaatgg tttctccctt
     4621 gctgttttga acaagggcat ggtctgcaat acccgattga atagcttctt tcagctgctt
     4681 ggcacgagta gctactgatt cattgtgaaa aaggttgtta tataaatctt ctagatagga
     4741 aagcacaacc actgcaaaca tatttgaagg aatgaggtaa ccatactgac aagcatcatc
     4801 acttggtcta aagcctgacc aggtcattcc tgttggtgcg acaggacttc cttttccctt
     4861 atgtgttagg gtatcttctt tgcgccaagt atcgcgttca aacaagtaag gagactgggc
     4921 gtggtcttgc tcaactgtcc ataggtcaag aatgagttct accccacgat gaaaatcgtc
     4981 attaaactga tcggtgcttc ctgtttcacg atagagcaag taagctagct gaatagggta
     5041 gcagagggaa tcaatctcat atttgcgttc ccaaatccaa gggttcattt gggtacgatc
     5101 tgtttggtgt ccttttccgt tagcttcttc gttgaaggca ttggcatagg gatcgataca
     5161 aatgtagcgg aattggcgtt tgaccaaacc ggcaataatc tttctaattt cttcatcttc
     5221 cttggcgagg aagaggtaag gttttatttg cgcagtggaa tcacgtaacc acatggcagg
     5281 aatatctcct gtcaagagaa agctagtgcc atcttcgtag gtttttaggg ttgtctctaa
     5341 ggtattggta aagctggttt tgaaattaag agcccaatca gggtgttggt ctccacaaag
     5401 gctttccacc ttggccataa attggctgac gctagttggg attggtcgtg tcatatcata
     5461 tcctccaaaa tgatatatca tttatcttcg gtttcattat aaatgataag ttaaactttg
     5521 tctataagtt tttgaaaagg tttacaactc ttttttgaca tgctatactg ataacgggag
     5581 taaagaatga caaaattatt atataaaaag atatatcatt tcattaaaga gcagatagca
     5641 tcaggtcgtt tgcaaattgg tgaccgccta cctactgaaa aagaattgtc agaacagttc
     5701 tcggttagcc gtattacttc taagcgagcg cttgtagaac tggagcaaga aggcttgatt
     5761 acgagaagtc gtgggaaagg aagcttcgta gctaaaaagc aagtgacgtc ccctgaagcg
     5821 aataaggatt tattgttgat tcttcctttt gcttctgatt atgaattggg agattatgct
     5881 aagggaatta tgacctccat tgctgaaaca ggttaccgtc tcatggtcca attggccagt
     5941 acagtaaggt tagacaccct gtccgattat gctgggatta tttattatcc tgaagatgtc
     6001 aatcacagta ttgatttcct gttttactgc gaccatcatc acattcctct agtcttgttg
     6061 gataaatctc tggatctttt tcagtttccg tctgttgtag cggataataa aggcggtgcc
     6121 tgtcaattga ctcagcactt gattgagcaa ggctgtgacc agattagttt tgtggcaaca
     6181 ggctcttttg gagaagtatc atccgttaga gaccgttatt tgggttactt agcggcaatg
     6241 gcagaatcgt ctctaaaatc ccattatttt cctaagacag agactgaaac aacagatgct
     6301 tatttggacc gtttggtgaa aaccttatcg gaaacgacag ccactaaaag ggggcttgtc
     6361 gtggaaaacg attggttggc tattcagctg attcaaaaaa tcacacaggt aggcttatct
     6421 attcctgatc aagtcgctat tgtaggcttt gataatagtc aagcctcccg tttgttgagt
     6481 cccaaattaa gcacagcagc tcaagatttt taccaaatgg ggcaagaagc agctaaagtc
     6541 ttgttgcaga ttattcatga gccagaccaa gcagtggtgt cttgccagtt acctgttcaa
     6601 gtatttatca gagaaagtag tcactaaaaa agacgttact agcatgagct gtttagctca
     6661 ggtaaaggag accagaaatg accatttatc acaccttaaa ggattatcaa gaagtcatga
     6721 caagaggaga ctatcttgtc tttgacacgc cattgacttg ccgatttatt ggccgttttt
     6781 tccgctttga aaatcaaaaa gccctgctgg cagaattggc aacgtcaaag tattttcagt
     6841 ggattgaaga aggccaagca gaggtgacga tgaaacactt ttttaaccgt cagctggcta
     6901 aggatgcctt taccctgaag gtttcagaag ataaggaaat cattattgaa agccaaaacc
     6961 ttagaggttt tcgttacgcc caagaagctt tgcttaaagt gatgactttc aaaggggata
     7021 aactttattt accgattgta tctgtcaagc atagtccttc ttttgctatg cgggggataa
     7081 ttgaaggctt ttacggaaca ccgtggacac gagaagagcg gttagattgc ctacgtttca
     7141 ttggaaataa aaggatgaat acttacatgt atgctccgaa agatgatgat taccaacgca
     7201 aacaatggcg agacctctat cctgaggatt gggtgactta tttcaaagag ctacttgcag
     7261 tagccaagga agaagggatt gatttttggt atatgattag tccgggactt gattttgatt
     7321 ataccaagga agctgactat cagttgctct atcaaaaatt gcaacaactt ctagcgttag
     7381 gtgtttgtca ttttgggctt ttgctggatg atattgacta ccaaattgta gatgctgttg
     7441 agagacgatt caaaaagact gcctatgccc aggcccactt ggcaactgag gttcatcact
     7501 ttttaaacca acaacatgcg gcgccagaat tggtgatctg tccgacagaa tacgacaacc
     7561 accatgattc tatctatttg caagagctgt cagaaaggat tcctaaagaa gtcgcctttt
     7621 tctggacagg cccaagcacg ctagcgagtc aaatctcgca agcagatatt gaaaccatgg
     7681 cagcgattta ccaacgtcct atcatcattt gggataatat tcctgttaat gattatcaaa
     7741 aagatccaga acgtctcttt ttaacccctt ttgctaatcg ctcgccattt ttatgccagc
     7801 cagactatca ggtcaagggg attgtttcta accctatgat tagttgggaa ttatctaagc
     7861 tgaccttgac agacatgagc cattatctgt gggatgctaa ccgttatcag cctagtcatt
     7921 cttggctaga aaccttaaca gactatacag aagatactga attagcccta gctttacagg
     7981 catttgcttg gcataatggc aatcgtcatc tgcacaggga ccttcctttt gaagtggaag
     8041 aagctctcct ggctaagaat gtatcaacgt tatcagcttg ggtagatgaa ttggtagaac
     8101 ttgtcaatac cttgagaaag ctagataagc cagcgtttca acaagctatt gctccttggt
     8161 ttgaacgtgt ggccaaagac caagattttt ggcaggctat gttaaatcag gagcctcagt
     8221 tggaaacact ctatgctgac ttacaagaag ataaacaccg cattggaagt gacattccaa
     8281 gtcgttacta tcgcatctac tatcaacagc aagataaact aacagctaat caaggtcaag
     8341 ttacccaagc aagacctgaa gattatgctt agtcatacga gctagaaagg aatgatatgt
     8401 tagcatttcc aaaagatttt tggtggggag gagccacttc aggccctcaa agtgaggggc
     8461 gttttgctaa gcagcaccgc aatctctttg attattggta tgagcaagag cctaccttgt
     8521 tttacgatta cgtgggacct gatacggctt cggatgccta tcatcagatt gaatctgatt
     8581 taaccttgtt agcttcacta ggtcacaatt cctaccgcac ctccattcag tggacacgtt
     8641 tgattgatga ttttgagcaa gcgacgatta atcctgatgg ccttgcttat tataatcgag
     8701 tgattgatgc ttgcttggcc aatggtattc gtcctgttat taatttgcat cattttgacc
     8761 tgccaattgc cctctatcaa gtctatggtg gctgggaatc aaagcatgtc gtggacttat
     8821 ttgtcgcctt tagcaaggtt tgttttgagc agtttggtga tcgggttaag gattggtttg
     8881 tccacaatga acctatggtc gttgtggaag gctcttatct aatgcaattt cattaccctg
     8941 ctatcgtgga tgggaaaaaa gcagtccagg tcgcctataa tctagcccta gccacagcca
     9001 aggttattca agcttacagg cgaggtccag cagaacttag tgatgggcgt attggaacca
     9061 ttctcaatct gacgccagcc tacccagcta gtcaatcaga agcggatacg gctgctgcgc
     9121 attttgcaga actttggaac aatgacttgt ttatggaagc tgctgttcat gggaaattcc
     9181 cagaagaatt ggttgcggta ttgaaaaaag atggggtttt gtggcagtca acgccagaag
     9241 aattggctct tattgctgag aatagagtgg attatttggg attgaatttt taccacccta
     9301 agcgcgtgaa ggctccggat gtcattccag tgattagccc atcttggagt cctgaatggt
     9361 attatgacgc ctacctgatg cctggccgcc gcatgaacgt ggataagggg tgggaaatct
     9421 atcctgaggc tgtctatgat attgctgtca agatgcgaga ccattacgac aatatccctt
     9481 ggttcttatc tgaaaatggt gtagggattt caggagaaga ccgttaccgt gatgaaactg
     9541 gtcagattca agatgattat cgcatccgat ttttgaaaga acatttgacc tacctccaca
     9601 aaggaataga agcaggctcg aattgttttg gataccatgt atggacgcca attgatggct
     9661 ggtcttggct caatgcctat aaaaaccgct atggccttgt ggaaaataat atccataccc
     9721 aagtgcgacg acctaaagct tcagcttatt ggtttaaaaa agtagctact cataactgct
     9781 tgatttcatt aaaagtaatg gaggagtttg gaggctctgc ttctcacctc agtgattgaa
     9841 tgtgtgaatg tctcctttga gacaagtgaa taatgatgtt accaatgatt aaacaagggc
     9901 ctttttcctc atgttaagag gatcgaggct cttgtatttc tttggaaagt gttggtattt
     9961 gccgaactag atataagcgt tctgtctggt ttagcattat ctatccatct tgggtaattt
    10021 ggctcttcaa ttctttagag tatcttggct aaggatgctt gtttttcatc ttggtacttt
    10081 aggattttct ttcatatttt tgggccagct aaaagcccct catggtttct agtgactgtt
    10141 tgttatgtca ctgtccacga taaggggact atatgagtga gttattaagt gaggctttgc
    10201 ttattggcaa taactagcat agcttctaat agctcgagca ttaactgatg accaattcct
    10261 ttaacagaag cgtcagcaac aatgacatca acaaagggac caggcatcag gctgtgttca
    10321 tcagcagaga gtcatttagg cagaaagcca gtatcgttgt agctaatcag caatcctttt
    10381 aggatgtctt tgtagatgtc agaggcgatt agggacaata ggggataaga gggatttagc
    10441 ggtccatggt taggcctcct tgtgttcttg gtaaaaatca atggcagctc ccagaagatt
    10501 agcttcgttt ttaaagtgac aaatagctaa ttggggtcta aggtcggaga tttcgacggc
    10561 ttggtagagt ttgtctagtt cagcttcaat cgctgacagt agaaagtcag cttgggaaac
    10621 accacccccg attaaaatca gttgaggatc aaaggcgtgt tgaatgttaa aaataccgat
    10681 ggctaaactt tggagaaaga cctgcctttc tttgattgct aggtggtctc cttgttctgc
    10741 gagggcaagg acagctttgc cagtataatc ttttccgtta ttgacgattg cactatagcg
    10801 cttggccata tttaccactg tccctaactg actaaaggtt tggtagcggt cgttcatgat
    10861 catgaaacca aattcgcccc caaataagtg ggcaccatgg taaatcttgc catcaatgac
    10921 caagcttcct ccaactccag taccaagaac gagcatggct aggctactag cgccttttcc
    10981 tgcccctaag gtagcttcag ctaaggcggc acaattggca tcattttcga tagaaatagg
    11041 gtaatgtagc cgttcttcta aggcctcttg tattttaaaa tggtgaatgt agggaatggc
    11101 gctggctcct tcgataatac ctttttcctt gttaaccgct ccaggagcac taacggcaat
    11161 acctgagaaa tggtaagcag agtaacgagc aacttcttgg tcgagtaatt ggtaaaactt
    11221 ttccagacta gagggagtgg gaaagctgct ttgctggctt aattggccat tgtgacagag
    11281 ggcaaattta agggaagtgc ctcctatatc aagacataac aagctcatga ggtgttctcc
    11341 tttattgtag tagaatagac gatcatgtct catcttagta tgacagattt aagcaacaat
    11401 gaaaaggctt tctaagtgat tctatatttt gtgctatcat acctataatt tgtgttatca
    11461 tacctatatt ttgttgcttt taaataatga aagcgcttga tatactgtaa ctataacgtg
    11521 attaacacaa agggagattg ggaaatgaca agacaaacca acaaaaagac atccttttgg
    11581 aaaaatgtga ttaaataccg ggccttgctg ttaatggtat taccaggttt catctggttc
    11641 atctttttct tttatattcc agttttagcc aatgtggttg cttttaaaga ttttcattat
    11701 tcagcagggg gctttatgga aagtttgaag gagagccctt gggttggttt ggctaacttc
    11761 aaatacctct ttgcttccaa agatgcttgg ttgattacca gaaataccat cgcttacaat
    11821 gtgattttct tactctttaa tgttttcttt gcgattgctt ttgccattat catgagtgag
    11881 ttacgaaata agcgaacggt taaggtctat cacaccatgt ccttattgcc ttatttctta
    11941 tcatgggtgg ttattgaata ctttgtttct gcctttttaa atacggataa aggtttcatt
    12001 aatcagctct tgactggaag tggaggagct ccaatcaagt ggtattccaa tcccacttgg
    12061 tggcccttga ttttactttt catgagtgtc tggaaggggc taggctacaa tagcattatc
    12121 tactatgctt cagtcaaagg gatttctgat acttattatg aagctgctat ggtggatggt
    12181 gcaagcaaat ggcaacaaat taggaacatc accattccac aattattgcc aatgatgtct
    12241 atcctattga tcattaatat tggtaatatc tttaaatctg atttcggact tttctacgtt
    12301 attcccaaaa attcaggccc actttatgac gtgaccagtg tgttagatac ctatgtctac
    12361 aatgccttga ctgctacagg agatattggg atggcatcgg cagctagcct ttatcagtca
    12421 gtagttggga ccagcatctt gttagtcacc aatgcgattg ttcgtcgcat ggaccctgat
    12481 gcatccttgt tttaggagga aaccatggag aaaaagaaaa aagtcgaaaa ggttaatgtt
    12541 agaacctttg atcgaaaaac caatgctatc tttaatattc tgattggtct gtttgccatc
    12601 tcttgtatca ttccttttat cttcgtgatt attatttcct tcactgatga aagttacttg
    12661 attaatcatg ggtatagttt ttttccagat gtctggtcaa ctaaggctta ccagtatatt
    12721 tttcaaggag ccatgtccca tagaatcatg aggtcatttg ggatatccgt gtttattaca
    12781 gtggtgggaa cctttattaa cacgaccatg acatcaacct atgcttatgc gatttcaaga
    12841 ccttatttcc catacagacg tttttttact gtttatgcac ttatcaccat gctctttgca
    12901 ccagggatgg ttgctaatta cttggtggtc agcaatctcc ttcatttgaa ggatacggtt
    12961 tgggccttga ttttaccaat ggctctgggc ccatttggca tcttggtcat gagaacgttc
    13021 tttaaaaaga cagttccaga tagcattatt gagtcggctc gtatggatgg ggctagtgaa
    13081 tggatgattt ttatgaaaat tgtcttacca ttagctgttc cagggattgc caccatcagt
    13141 ttattttctg ccttaactta ttggaatgat tggtttaatg ccttgcttta tgtgcaaagt
    13201 gaaaacctct acccaatgca gtacttactg atgaaaattc agagcaacct acaggccttg
    13261 gcacaaaatg ctggcatgag tgcacaaatg gcagatagct tagcatcgct gccaaaagaa
    13321 tcagttcgta tggccatcgt ggttattgca accttgccga ttgccttgac ttacccattc
    13381 ttccaaaagt actttgttgg tggtttgacc attggtgggg tgaaggaata ggacttaagg
    13441 aactggtcac aagaccagat tttatctaat cattagtaac aaaagaaaac attttttagg
    13501 aggcttcact tatgaaaaag tggcaaaaaa tcgtgtgtgt gactggaact gtgcttgcag
    13561 cgtctagttt agcagcctgc gaaagcaagt cagcatcaaa ggatagtgat gtcaaattat
    13621 tgatgtacca agttggtgac aaacctgata acttcgatga attgatgaca attgctaaca
    13681 agcgcatcaa agaaaaaaca ggtgcaacgg ttgaccttca atacatcggt tggggggact
    13741 gggatgataa aatgagtacc atcattgcct ctggtgaaaa ctacgacatt gcttttgcca
    13801 ataattatgt ggtcaatgca caaaaaggtg cctttgctga tttgacaacg ttaatgccaa
    13861 aatacgctaa gaaaacgtat aaaaacttag acccagccta tattaaagga aatactattg
    13921 acggtaaact ctatgccttc ccagtagatg ccaacgttta tgcccaacag atgctttctt
    13981 tcaataaaga actagtggac aaatatggcc ttgacatttc aaacattaag tcctatgcag
    14041 atgctgaaaa tgtcttaaaa caatttcacg aaaaagaacc aaatacagca gcttttgcta
    14101 ttggtcaagt ctttagtatg tcaggtgact atgactaccc attaaccaaa aaccaaccct
    14161 ttgctgtgaa aattgatgaa ggcaagccga ccatcattaa tcagtatgaa gatgagtcct
    14221 ttaaaaacaa tctccgcttg atgcacaaat ggtataaaga aggtttgatt ccaacagatg
    14281 cagcgaccaa tacagaaggt tatccccttg aaggaaacac ttggtttatg cgtgaagaaa
    14341 cccaaggtcc tatggactat ggcgatacta tcttgaccaa tgctgcagga aaagacatcg
    14401 tgtctcgtcc attgactaaa ccgctaaaaa ccacatcaca agcacaaatg gcaaactttg
    14461 tggtatcaag cgtatctaaa aacaaagaaa aagcagttga agtcctttct cttcttaata
    14521 gcgatcccga attgttaaat gggcttgttt atggtgtcga aggcaaagct tgggaaaaaa
    14581 ttggcgacaa gaaaatcaaa ctgctcgatg gctatcaacc aaaaatgcac atgggtgctt
    14641 ggaatactgg taacaataaa atcctttaca ctcaagaatc tgttactgac gacatgattg
    14701 ctaaacgtga ccaatcaatc aaggatgcta aagaatctcc aatccttggt ttcactgttg
    14761 acacaaaagt tattaaaacg gaattgtcaa atatttcaaa tgtcatgaac cgatataaag
    14821 caagtatcaa tactggtaca gtggatcctg acgaagccct tccaaaactt cttgcagacc
    14881 tcaaaggtgc tggttgggac aaggtgcaaa aagaagttca aaaacaattg gacgactttg
    14941 tagctaaaga caagtaaggc attcattttc ttgattataa gttgagggct gggaaatctc
    15001 agctcttttc ttctgatgag tggaaaaaac ggatcatcaa ataagtggaa gttataaagg
    15061 gagaaaaatt aatggaaata atgttactac tcttagtaaa gcggtttcca aaagcctata
    15121 aagtgtgcta tattagaaga gtctaaacta ttaatcagag taaaactaat accagcatct
    15181 tactaggggt gctaaaacta gtctcgtagc tgctggtccc tgaaaccgct ataagtgtta
    15241 acaagaaaaa gaggactatc atatgaatgg cattgaaaaa acctgctttt taatttggaa
    15301 aatgatccaa ttgaccctca tttttcatct cttgtcctta gctggactga ttgtgtttgg
    15361 ggtggggccc gcctggcaaa ccattgtcac cctattttta aaaacacctc aagaagaaaa
    15421 ccactattct ttgaaaaggg ctgttcaact ttggaaatct tatttcaaag aagctaatct
    15481 aagatttggt ctttttgccc tgactttcct ttttttaacc tttaatttgc attgggcagt
    15541 acagtttcca tccttgtttt ggtttaccgt gtctttcttg attgtaatag ccatggtttg
    15601 gctaaccatg acctatcttt acatggtttt ttatgcagtg agctatgaga ttaacctttg
    15661 gaataatatg aaattagcct ttatcagcgt ctttttaagt ttcaaatcct ttttactcat
    15721 gctgagtgtt ttgctgggaa ttaccttaat gacttggcaa tacaaaggcc tttatctctt
    15781 tttaaccttc ggagctctag tcttttgctt ggactttgtc actaaggcta atcgtcgact
    15841 agtagatgga gtgattgatg agtgttaaaa cattgggaag gtatgtgact aaccacttta
    15901 agcagggttt tttgattaat cgactgatga aattatatag cctgctaatt gtaagttttt
    15961 tcactttatc agctattggc ttgagcagtt attctattag tagtacttat aaaagggtgg
    16021 atgctgaggc gcagatgcga ctggaggaga ctctaggtag attgcagtca caaaacgata
    16081 ttaccctacg ggttttagac cagttggttg gccgttcaga agattaccat aacctctatc
    16141 aatatatgac gttaacccca aaccaatatt tttcaaacgt ttttgaagac tgggaaaaag
    16201 gaaaagacac ggtcctattt tctgaagagg tgaggcgtct ttttgacttg taccctgatg
    16261 tgacttctat gaccctactg ctagaggaca gtagtgctta cctgtacgcc gacagggtca
    16321 ttaagacggg gcgtttgctt tatagcaagc ctgaaaaagt aaccggaaat gttttagttc
    16381 gctctattcg aaatccagaa tcaggagatg tgacaggtcg tctttacctg acttttgaca
    16441 aaccaccgac tttggtagag acacagcagg atcactattt agctactttt gcttttgatt
    16501 actatggccg caaactcttc catcaaggag gtcggagatt tcctcattta gaggctgagg
    16561 ttaaaaaggc tatcaaggca gatcgggcag ctgatcttag taatcttagc aaggcttatc
    16621 ggatgcagta caatcgctcg ggagacctct tagcttatgt ggctgtcaga aaatcctatt
    16681 tattagcgga agctgtcagg acagtttttg tgtatggcct tgtttctcta cttttagctt
    16741 ggctgttatt gcagttgctc tttagggttt tccgaaatta tatccagcag gtttctgaaa
    16801 ttacggatac tgttgaaatg gttgcagcag gcgatttgtc tttaaccatc gacaacagcc
    16861 acatggaatt ggaactgtat catatctcag aagccattaa tcagatgttg gccagcatta
    16921 aagcttatat tgacgaagtt tatgtgttag aggtagagca acgagatgcc caaatgagag
    16981 ctctgcagtc tcaaatcaac cctcattttt tatacaacac gttagagtat attcggatgt
    17041 acgcccttag ttgtcaacaa gaagaattag cagatgtcat ttatgccttt gcgagtctgc
    17101 ttcgcaataa catcagccaa gataagatga ccaccttaaa agaagaactg gctttttgtg
    17161 aaaagtacat ttacctttat caaatgcggt atccagatag cttcgcttat catgtaaaaa
    17221 ttgatgagag tattgctgac ttagccattc ctaaatttgt cattcaacct ctcgttgaaa
    17281 attattttgt gcatgggatt gattatagtc gccatgacaa tgcactaagc atcaaggctt
    17341 tagatgagac ggatcatttc ttgattcagg tgcttgataa tgggcgtggt attagtcaag
    17401 agcgcctagc agatatggaa agaaggcttc aagagcacca aacaacaggc aatagctcta
    17461 ttggtttgca aaatgtttac ctccgtctct ttcatcattt tcgagacagg gtttcttggt
    17521 ccatggctaa ggagccaaat ggtggcttta tcattcaaat taggattaga aaggatgctt
    17581 gatgtataag gtactattag ttgacgatga atacatgatt ttacaagggc tcaccatgat
    17641 tattgattgg caggcacttg gatttgaagt ggttcaaaca gcaagatcag gcaaggaagc
    17701 cttagcctat ttgactcagt acccagtgga tgtcatgatt tcagatgtta ccatgcctgg
    17761 aatgacagga ctggatttaa tagaagcagc taaaacctat catcctcagc tacaaacctt
    17821 gattttatct ggttatcaag aattctccta tgtgcaaaag gctatggaat tagaaaccaa
    17881 ggggtatttg ctaaaacctg ttgataaggc agaattacag gctaaaatga aacaatttaa
    17941 agactgcttg gatgcccagc aggcagaatc aatccggcaa gaagcctatc atgacagcct
    18001 tttgaccctt tggcttacag atgaattgag tgagaaagaa tttcagcagc taagtcaagg
    18061 cttgccagcg gctgctttaa ctggttttac agtgctgtat ttagactgtc aagagtggca
    18121 agcagccatc gatgcttatt ttgaacgaga aggtcagcct ttttacctca aaaaagaaga
    18181 agataagaag gtttatctgg ctgtcctact tggtaaaact gaccaagcca aggcatttac
    18241 ctatgaccta caaaagcgat tctcaagaaa catgcatcaa cttattttgg gagaaacagt
    18301 tgatgactgg gaaaatgttt atgaaagcta caatcaggtg cttaagagcc tcttttacaa
    18361 ttgtgaagtg tcaccaatta gagcaaaaga taagtccaaa attgaactac cggagtcccg
    18421 tttgcagttc tttgctttta acaaggcgtt gatgattggg gacgaaccta caatcttgtc
    18481 taaattagaa gctatttttg acgagatgaa gaccttgaac ttttcaccag aggatgtgaa
    18541 gcatgtttcg ttcttgttgt tttctgacat ttaccgtcaa tttcccattt tagataaaat
    18601 gacttatctg tccatggtaa aaactattca tgacagccaa tccattgatc gcattttgag
    18661 ggaactaaaa aaagtgttgg atgtcaccaa tcagaacaat tcccctgaaa aacgctattc
    18721 agatttagtt agtgaaacca ttgattgcat tcgcaaggaa taccatcaag aattaaccct
    18781 taaagctatc gctgataggt tacatgtcaa tggggtttat ttagggcaat gctttaaaaa
    18841 tgaaaccgag cgtagtttta cccagtactt aaatcatgtt aggattcaaa aagcgcaaca
    18901 gttattgctt tacaccaacc aatccatcaa tgaaattgct tatgaaacag gttacaatac
    18961 caatcattat tttatcaaga tgtttaaaaa gttgaatggt ttatcgccaa aagagttcag
    19021 agatcgttac aaggacaatt accaagctat caagggtgac cagtaacgtt aaacaggaga
    19081 ttgatatgac atcagatagc aaggagcaga cagtgactgg ctaccaatac cactatattg
    19141 atcaagaagg aagaaagcag ccctttaatc aaggctggcg ttttcttatg gcagatgtgg
    19201 cgtgtgctca ggacccttct tttgatgatt cgaactggca ggtgattcat ttaccacatg
    19261 atttcagtct tacgcagcct tacacaagaa atggtgaagc tgaaagtgct tataaattag
    19321 gtggtgtcgg ttggtatcgg cattatctcg tgttagatga ggttttagct ggttatcatg
    19381 ttgccattac ttttgagggt tcctatatgg aaacggaaat ttatgttaat gggcaattca
    19441 ttggcaaaca cttaaatggc tatcaggaat ttacctatga tatctctgat gtagtgactt
    19501 ttggagctga aaatcttctg gctgtccggg ttgaaaacaa ggttcccagt tctcgttggt
    19561 attcaggcag tggtctttat cgtgaagtga gtttgtctgt tcttccccag cttcattttg
    19621 tggcagacca ggtagcaatg accttggctg atactgctgt gcaggagaaa gggcaacaga
    19681 aagttgattt acgctttgcc cttaatcagt ctattcagac ttgccactac caattgagcc
    19741 tctgtttatg ggaacaatca cattgctcaa aagacaagaa actcctttat caagaaacag
    19801 aagtgcctct agctgatttg gctttccaaa gacagtatgg tcttacactg tctttggaag
    19861 aactccaatt gtggtcacca gataatcctc acctctacga tttagaactc actctttact
    19921 atcaaggtca ggtcattgat tgcttttgcc ttgaaactgg ttttcgtcag ctgactttta
    19981 tggccaatca ggggcttttt gtgaatggca gagctgtcaa attaaaggga gtttgtttgc
    20041 atcatgacca aggaggccta ggagcttgtg cttacgaaga tgccctagcc agacaattag
    20101 tcctgttgaa agacatgggg gctaatacca ttcgtagcac ccataaccca agtagtccca
    20161 aacttaggca actagccaat cgtctcggct tttttgtgat tgaagaagcc tttgacacct
    20221 ggacctatgc caaaaatgga aacgttaatg acttttcaaa ctatttccat cagactatcg
    20281 gaactgaaaa tgcaaactat cttcaaagag tgaggtcacc tgaaacgagc tgggcccagt
    20341 atagtattga ggccatggtc tggtctgcca agaatgaccc gtcagtgttg atgtggtcga
    20401 ttggtaatga attgatggaa ggtttttcag ccgatgtcag tcattaccca gagctgacaa
    20461 gacaaatgtg tcaatggatt gctgctattg acacctcacg tcccattacc tttggggaca
    20521 ataaattaaa agaagctgac ttttgctggc atgaggaagt gtcgcaaatg gcaacttttc
    20581 ttagtcaatt agaccatccc cagggactca ttgggttaaa ctatgctaat ggaaaagact
    20641 atgataagct tcatgaggag cactctgatt ggctgttgta tggttctgaa acagcctcag
    20701 ccattaccag cagggcttat tataaagagc caaaaaaagt tcttgactcc ggttatcacc
    20761 taacctctta tgatcatgct aaggttgatt ggggtgcctt cgctagccag gcttggtatg
    20821 ataccattac ccgtgatttt gttgctggcg aatgcgtttg gacaggcttt gattatttag
    20881 gagaacctac accctggaac aagatcgaca gcggtgttgt aggcctttgg ccttctccta
    20941 aaaatgccta ctttggcatt ttagacacgg caggctttcc aaaagatagc tattattttt
    21001 accaaagtca gtgggcgcag gggcagacca ccttgcacct gttaccagtc tggcagaagg
    21061 accagctttg tttcgatgag cagggattgg tggaggttgt ggtttatagc aatgctgcca
    21121 gtgttcaact gatgtttgaa gatgagcagg gcaacttaac agattatgga aggaaggcat
    21181 ttcatactta tagcacgcca acgggacaca cttatcagct ttaccaagga gctgacgctg
    21241 ctaagaatcc tcacgagaac ctttacctga cttggcgagt gccctatcaa aaagggttgc
    21301 taagggcagt ggcctacgac atttctggta aaagcattcc aaaaaccagt ggacgttctc
    21361 aggttagaac ctatggttca gtggcaaagc tatcttggaa agcctttgaa gcacctattg
    21421 acgctccttg ggaattgctt tatctggatt tatcactctt ggatagccga ggtgaattgg
    21481 tcagccatgc tcaagattgg ttgcaggttc aagtagaagg accagcccgt ttgttggctc
    21541 ttgataatgg gaatccgact gaccacactc cttatcagga gcctctgcgc caggcatatg
    21601 ggggaaaatt attagccatt ttagccctca ctggagagga gggacacatt aaggtcacgg
    21661 caaaagtctc gcagcaactt agcagtgttt ttcagacaca agtagcctgc cgtgccccca
    21721 aagaacactt agtgacacta cctttagaga aatatcagtt taaaaagagc cgactgacaa
    21781 gtcaacaaga aattggtcaa tcttgtctaa agaccacaca attcctttct gttttggaga
    21841 acaaagacac ccagcaaaaa gcaggtaagg ttagcacacc tcacattctt atcaccagtt
    21901 accagcacta tttatctctg cccttgcagg tttgttctca ggcatttgat tttaaaaaag
    21961 gcgacgatat cttcttgcct cagtggctcc aagtcaaaga tgcccaaggt tctcttgtga
    22021 aaaaagcttt ttcaatgacg tggtacctaa aagagaaaat ctttgccaag cctattcttg
    22081 tcacaggcta tgtttcttgt tttgggcaat caataccggt tgaggcaagg cttaacctag
    22141 cacaagctag caaaagacgg caccaggatc tcagtttagt cgccaagcaa tccctgaccg
    22201 caagtcaaaa aaggctaact tattgctacc aatatgatac ggtgcaagcc ataggagcca
    22261 ttagcttgca aaatcacaca gacacatctc ttagtgtttc tgtggcaatc agttatggta
    22321 gtcagagctc tattaagcaa gaaaccttga tgctagctga tagccacttg agccagttag
    22381 aagaggttat tttcttcgat gatattttga ctgttttgta tctatccgtg acccttgaag
    22441 tcattgaagg acctgaccta actgaagaca gtattcaaat tggcctttgg gatttagcat
    22501 gaagcatgag tagaagaaga cttctttggc accaggtctg atgaattctg taaaataagg
    22561 tataataaaa aacaaatagg cgtcctttat tacgtgaaag gcgcctttac aaggttgaaa
    22621 tggaaagtga gatttctaaa tgtcagaacg tttatcaggc cacaccctgc tggtctcttt
    22681 gcttgctact cctattcgtc atagtttgtc tcccaaaatg cacaatgaag cctatgctaa
    22741 actggggctg gattatgcct accttgcttt tgaagtaggg acagagcagt tagcagatgc
    22801 cgtgcaaggg attcgtgcct taggaattcg tggctccaat gtgtctatgc caaataaaga
    22861 agctatttta cccttattgg atgacttgtc cccagctgct gagttggtcg gtgcagtcaa
    22921 tacggttgtc aataaggatg gtaaaggaca tctcgtgggg catattacag acggtatagg
    22981 agcgctcaga gccttggctg acgaaggagt gtcggtaaag aacaaaatca ttaccctagc
    23041 aggagttggt ggggctggga aagctatcgc tgtccagctc gcttttgacg gcgccaaaga
    23101 ggttcgtttg tttaaccgac aagctacacg tctaagtagc gttcaaaagc ttgtgacaaa
    23161 actgaaccag ctaacaagga ctaaagtaac ccttcaagac ttggaagatc agactgcctt
    23221 taaggaggcc atcagggaat ctcatctatt tattgatgct acaagtgtag gaatgaagcc
    23281 tttagaaaat ctaagtttga ttaccgaccc cgagctcatt cgcccagact tggtggtctt
    23341 tgacatcgtg tacagcccag cagaaaccaa gttactagcc tttgcccgtc aacatggcgc
    23401 ccaaaaagtc attaatgggc tagggatggt gctttatcaa ggagcagaag cttttaaatt
    23461 aatcacaggt caagacatgc cagttgacgc tattaaacct cttttaggtg atgagtaact
    23521 caaccctttt taaagaactt tttaagtaag cttaattatg atatattaat aagagatgga
    23581 gcagtcatca tgggctgcta aaattctttt tgcgagagct gtttttaaga gcaaaaagct
    23641 tatttgaaga gccttatccc taacgtgata caagtcatag ttcttttgag gtctgccaga
    23701 taacctaccg tttttttaga aaagaagctc ttataaaagg aaaagttcaa tgaataaagg
    23761 agaatagcaa tggtaacaaa ggaagttggg catgactttt tagcacgtct aggaaaaaaa
    23821 cgcttaagac ctggcggtgt tgaggcgaca aactggttga ttgaacaagg acaattctct
    23881 caggattgtc acgttttaga ggttgcctgc aatcgctgta cgacagcaat agagttagcc
    23941 aaaaccttcg gttgtcagat tgatgccgtt gacttaaacc ccaaggttgt caaagaagca
    24001 caagaaaggg ttgctgttga ggggttaacc cgtcacatta cagtatccca agccaatgct
    24061 ttatcactac catttccaga taaccatttt gatattgtga ttaatgaagc catgttaacc
    24121 atgttgaacg cccaagccaa ggagaaggca ctcaatgaat attggcgtgt gttaaaacca
    24181 ggcgggcgac ttttgacaca tgacgtagct tatgaagatc caaaaacaag acaagtactt
    24241 gacgagttac gtcaaacgat tcatgtcaat gttgctccct tgtcactaac aggttggcaa
    24301 gccttatatc aagaacacgg tttttctcaa gtgacttatc accatgggaa aatgtctttg
    24361 atgacacctc gtggaatgat taaggatgaa ggtcttatga acaccctgaa cattatgaga
    24421 cgaggcctta agaaagagaa cagacaacag tttttaaaga tgcgtcgttt ttttaccaga
    24481 acaggcaaaa atctctgcta tattgctgta aaaagtgtaa aataaacctt agaccaacct
    24541 gtccatacaa ctaaacagac catagaggag ttcgaagatg tcttatatca ataatattga
    24601 gcacgcgaag gttcttgatt tgacgcaaga agtaatgatt gagcaagatc aaatgttaag
    24661 tagaacacta gtccaaaggc aagacctagg aataacagtt ttttccttag ataagggcca
    24721 agaaattggg cgacactcat caccaggaga tgccatggtt actatcttaa gtggcttagc
    24781 agaaatcacc attgatcaag agacctaccg agttgctgaa ggtcaaacca ttgtgatgcc
    24841 agctgggatt cctcatgcct tatatgcagt agaagccttt caaatgcttt tagtagtagt
    24901 aaaaccggag gcttaacatg attgaaaatc tctctcaatt ggtgcaatat catgatcatc
    24961 aggtggctag ccgatccttg agccgtctac tgggattatc tcaatctatt gttttatatg
    25021 ccatggccca aggagaaagc attagtcaag agactagtcc aagagataaa gtaatacttg
    25081 ttttagaagg tcagctcatt ttcgatttag aggatcaaaa acaggtccta actcaagaaa
    25141 gtctcatagc catacctgcc caaaaagtcc atcatttaga agctaaaacc gattgtaaac
    25201 tattacaagt gcttttagat taagggctta ttgtcaactg tattgggtgg cctatggaaa
    25261 agcaaaagag aaggtgacgc tcatcttctc tttttacgta ttcaaagcga tgacaatacg
    25321 aattttaaag cttggctttc tcatctccag attaagccct ttagcttggt ttattttaga
    25381 attcactaaa aaaaccttaa tgagtttcct atctccttat aggtttctca ttaaggcttt
    25441 ttagacgaaa taattgaacg acaggcaaaa gaaagatctt tttacgagtg ataccgttct
    25501 cctatagttc cataaatcct ctttttgatg ttgctgttag ttattcttag ctaaaagtta
    25561 tattgttatc aagacttgtc ttgtagtaac atgttttagg agttccagga taccgttatt
    25621 ctcttagcaa gcttaactag ctagagtgga gtcactattt ataataaact gtcgagcttc
    25681 tcgacaaatt ggtgatcgtc tttgttaatg gctagggtgg taaaaccggt ttctgtgggt
    25741 aatataaaga taatatgttg ctcagaattc ttcttatctg ttttaaagac gtcaaagagg
    25801 gatgtcgcag agacatcagc tctttcaagt gttgttggta actggtaacg ttcaaaaact
    25861 tgaaggaggc tatccaagtc ctcttttggc aagagtcctt tggaaacagc cagtttagca
    25921 tcaaccacca taccgatagc aatggcttca ccgtgatggt aaaaatcatg gtgacagagc
    25981 gattcaatcg cgtggcccaa ggtatgccca aaatttaaag acatgcgcaa accattttca
    26041 aattcatctt gttcaacaat ccgtttttta ttggaaatgg attggtaaat cagacgttca
    26101 agtccctgct tatcggtttc tttttgaaaa tcacgtaact gctgaaaaag cggtctatca
    26161 tgaatgaagc cgattttgag catctcactg atgccgcagg agaattcccg ttgcggtaag
    26221 gtttccaaaa aggtggttga aataatgaca gcttctggag ggtaaatgct gccaatcata
    26281 ttggttagac ttttaaaatg gactccaact ttgcccccga tactgctatc cacctgacta
    26341 agtaaagtcg ttgggatttg aatgtaggga ataccacggt aataggttgc agcgacaaaa
    26401 ccacccagat ccccaacaac accaccacca atagtaacaa tcatatcttt tttggaaaaa
    26461 ttttcagcga tgagctggtc gtagatagca ctgacacgtt ccaaggattt ggattgacct
    26521 cccggggggc agacatggac aaaagcgttg tattgctgag caaattcttc aaagagagtc
    26581 tgatacaagt ggtagaccgt ttgatcggtg ataaacaaga gtttacgctg cttgcgctca
    26641 ccaaggcaat ctgctaaggt ttttaaaaca tggtcggtaa acaggatatc ataatccttg
    26701 acccgagaat ggacatgaag tgtttgtggc ataagcactc ctttcatcag tactaggtaa
    26761 cagagataac gcagtgatat ttagagctta atcttgccaa agttcctgcc ccaactgttc
    26821 taattgctta taatcaattt gctgggcagc atctgacagc gcatggtcgg gatcaggatg
    26881 aacctccatc atgatgccat tggcaccgac agccttcgct atcttagcag caggtaagag
    26941 taaatctcgt ctgcctgtcg aatgagacac gtccacaata atcggtaaat ctgtcttttg
    27001 ttgaataata gggactgcca taatatcaag catatttcta gtctccacat cgtagccacg
    27061 aacgccacgt tcgcataaga taatgttaga ctttccagtg tcttgtaagt aggaaagggc
    27121 gcccaaatat tcctcgatag tagccatcaa gcctcgttta aacaaaatag gcttatcgat
    27181 gtgtgacaag gtttttaaaa attcaaaatt ttgcatgttg cgggcaccaa cctgaatgac
    27241 atcgagatag tcataggctt cttctaattg gcgctcggac atgatttcac tgaccgatag
    27301 taagccaaat tcctgacaaa cctcatgcaa gtaacgaatt ccttgcaggc caaggccttg
    27361 aaaagaagca gcagatgttc ttggtttata ggcgcctcct ctaaaatagt tgtaaccaag
    27421 ttttttggca cttgaagctg ctaatctaat gtgatcataa gattcaatgg aacaaggtcc
    27481 aacaataaaa ttattcttag aacaagtctt attttgaaag tcagacataa tgtcaatcat
    27541 tcgtttgatt cctttactaa ttctgtatgg cgtgctctat gggataacgt cttatttagg
    27601 aatacaaaat ctcgttgtta tccatgaagc acccttagcc tgtgaaatga taccataaac
    27661 aagatgaaat agcaagtaaa ccctagattg tctatgttct tagtcatttt tattaaaaaa
    27721 ggcttactag ctataaaaaa atactgccct aaatcttatt aggacagtat ttttttatag
    27781 atgccaccac cgagaatcga actcggaacg aagcattacc attgctttat tataccattt
    27841 aactacagcg gctacctttt tataatacta taagttatta attgatgcaa gcttttctta
    27901 aaaatatttc cttaattgtt tatcatcaaa atgagagtac cagttattaa taacatggca
    27961 caagaggcct gccttatggt ataatggtaa agatgatttc agaaagagga agccaagcat
    28021 gtcttatgaa aaagaatttt taaaagattt tgaagactgg gttaaaactc agattcaagt
    28081 caatcagcta gccatggcaa ccagtcaaga agttgcccaa gaagatggcg atgaaagggc
    28141 taaagatgcc tttattcgtt atgaaagtaa gttagacgcc tacgaatttt tactaggtaa
    28201 atttgacaat tataaaaatg gtaaagcctt tcacgatatt ccagatgagt tatttggagc
    28261 aaggcattat taaggacaag gagatacgaa atagtggcaa aacaaaaaaa tcgtaaaaaa
    28321 gcattgaagt tagaaatgaa gcgtaatggc ttgttaaaaa aagcaggtca agtttttgat
    28381 aaggcagttg aaagtgttga aacagccgtg gataaaacca tttctgctgg taaaaatctt
    28441 gtggaaaaag gcagccaaac ggttgaaaac ctgacagcaa gcaaggaaag acttactctt
    28501 gatgagtttg cagggcagcc agttgtagct ggtatccgct ctgaccttgt cgaaacccta
    28561 tatgcagaag gcatccattc agcgcaagct tttaaggagt ggacagaaaa agacctcctt
    28621 gctcttaagg gcattggccc agcaacggtt aaaaaattgg tcgaaaatgg cgcaagcttt
    28681 aaaaaatagt catcttctcg ttgcttaatg gctataaatt tgttagaata ttagcaatca
    28741 gaggtgcatt gagttggtct gttacagaga gcggtggtgt tgagaaatcc gcacagatgt
    28801 caaggctggt agctgaggat aagaaatcag atatgaaaag tagcagaccc attttatggt
    28861 ctgaaacagt ggtggtaccg cggaaatcgt aacacgaaat tcgtccctgt ccagtgtaag
    28921 ctggataggg attatttggt ttcatttgaa agaagatgat ggcttattgt taaaatgttg
    28981 ttagaagtat acaatagaaa aactttgccc aaagtggaaa cagaacggct gctattacgt
    29041 caacgaaaag gggacaaggc attgttccag aagcggcttc agctctgatt gaaattggtt
    29101 ttaccttact tgatttgcat aaaattgagc tgggttgcta cgactacaac aagcaaagtc
    29161 aggcagtggc tcgcaaatta ggctttacct tagaagccaa tgcccgtgac cgtaaggacg
    29221 ttcaaggtcg ccgctgtggc gacatgcgtt ttgggttact gagaagtgag tgggaggagc
    29281 agaaacaaaa atgaacctta tcatcattgg tgctcaagcg tcgggcaaga tgaccattgg
    29341 gcaagaagtt gccaggcaga caggcatgac tttgtttcat aatcacgatt ctattgattt
    29401 tgttttacgg tttatgcctt ggtctcaaga gagcactgcc ttgattgaac gtatccgttt
    29461 tgcttttttt gaaacctttg ctaagacagg tcaagacatg atttttacta ttgtaattga
    29521 cttcaatgat ccaaatgatg tagcaatgct tgaaaagatt caggctgttt ttcagtctta
    29581 tgaccaagag gttctctttg tcgaattaaa aacagatata gaggaacgtc ttacacgcaa
    29641 tcgcacagaa aatcgtctca aacataaacc tcttaaacgc aacatcgagt ggtcagaaca
    29701 agatattcaa tccacgatgg catatgctgt ttttaatcca gaagaaccac ccaaaacgct
    29761 cacgcattac caaaaaatca ataataccca tctcaccgct gctgaaacag cgcagcttat
    29821 tatccaaaaa atgactcata tcaaggagaa ctaagatgac agaactatca cctaaataca
    29881 atcctgccga ggttgaggca ggtcgctatc aaaaatggct tgatgcagat gttttcaagc
    29941 catcaggaga ccaaaaagca aaaccgtatt ctatcgtgat tccaccgcca aatgtgactg
    30001 gtaagttgca cttgggacat gcttgggata cgacgcttca agatattatc attcgtcaaa
    30061 aacgcatgca gggctttgat acgctttggt tgccagggat ggaccatgca gggattgcga
    30121 cacaggctaa agtcgaagaa cgtttgcgtg gtcaaggcat cacccgttat gatttgggtc
    30181 gtgacaaatt cctagacaag gtctgggagt ggaaagacga atatgccact acgattaaag
    30241 aacagtgggg caaaatgggt ctttctgtgg actacagccg tgagcgcttc acccttgatg
    30301 agggcttgtc aaaagcagtt cgcaaggttt ttgttgacct ctacaaaaaa ggctggattt
    30361 accgtggtga attcatcatc aactgggatc ctgctgccag aactgccctt tctgacatcg
    30421 aggttatcca caaggatgtt gagggagctt tctaccacat gaactatatg ttggaagatg
    30481 gctcacgcgc ccttcaagtg gcaacaacgc gaccagaaac catgtttgga gacgttgccg
    30541 ttgccgtaaa cccagaagat cctcgttaca aagatttgat tggtaaacat gttattttac
    30601 caatcgtgaa taaattgatt ccaatcgtgg gagacgagca tgccgaccct gaatttggga
    30661 ctggagttgt gaaaatcacg cctgctcatg accctaatga ctttgaggtt ggtcagcgtc
    30721 ataacttacc acaagtcaat gtcatgaacg atgatggaac catgaatgag ttagctggcg
    30781 actttgcagg catggaccgt tttgaagctc gtcaagctac cgttgctaaa cttgaagaac
    30841 ttggtgccct agtaaacatc gaaaaacgtg ttcactcagt aggtcactca gaacgttctg
    30901 gtgcagtcgt tgagcctcgt ctatccaccc aatggtttgt aaaaatggat gagcttgcta
    30961 aacaagctat ggacaaccaa gaaacagatg accgagtaga tttctaccca ccacgcttta
    31021 atgatacctt ccttcaatgg atggaaaatg tccatgactg ggtaatctct cgccaattat
    31081 ggtggggtca ccaaattcct gcttggtaca atgcagaagg tgagatttac gttggcgaag
    31141 aagcaccaga aggtgatggc tggactcaag atgaagatgt tcttgacact tggttctcat
    31201 ctgctttatg gccgttctca actatggggt ggcctgacac agatgtagaa gatttcaaac
    31261 gttatttccc aacctcaacc ttagtaacag gttatgacat cattttcttc tgggtgtcac
    31321 gtatgatttt ccaatcgctt gaatttaccg ggcgccagcc attccaaaat gtcctgattc
    31381 acggtcttat tcgtgacgaa gaaggacgca aaatgtctaa atcattgggg aacgggattg
    31441 atccgatgga tgtcattgag aaatacggtg ccgatagctt acgttggttc ctctcaaacg
    31501 gctctgcccc aggacaagac gtgcgtttct catacgaaaa aatggacgcc tcttggaatt
    31561 tcattaataa aatttggaac atttctcgct atatcctcat gaataatgaa gggttgacat
    31621 tggaagatgc tgaaagtaat gtggctaagg tagctgcttc agaagctgga aatgtgactg
    31681 accagtggat tctccacaat ctcaatgaaa ccatcgctaa agtgacagaa aactttgaca
    31741 agtttgagtt tggtgtggca ggacacattc tttacaactt catctgggaa gaatttgcca
    31801 actggtatgt ggaattgacc aaggaagtgc tatatagcga caacgaagct gaaaaagtta
    31861 tcacgcgctc tgtccttctt tatactttgg acaagattgt acgtctcctt cacccaatca
    31921 tgccatttgt aacagaagaa atttacgctc aatatgccca aggttctatt gtgacggtgg
    31981 actatcctgt ggttcgccca gcttttgaaa atgaagctgc gcataaaggt gtggaaaggt
    32041 tgaaagacct gattcgtgca gttcgtaatg cgcgcgctga agttaacgtg gcaccatcaa
    32101 aaccaatcac cattttggtg aagacagcag atagtgagtt ggaagacttc ttcaccagca
    32161 acgtgaacta catcaaacgt ttcaccaatc ctgaaaaatt agaaatcagc tcagccattg
    32221 ctgcgccaga gctggctatg accagcatca tcacaggtgc tgaaatctac ttgccactag
    32281 cagatttgct taatgtggaa gaagaactcg ctcgcctaga taaagaactg gctaaatggc
    32341 aaaaagaact ggacatggtc ggcaaaaaac tcggaaacga acgctttgta gccaacgcca
    32401 aaccagaagt catccaaaaa gaaaaagaca aacaagccga ctaccaagcc aaatacgacg
    32461 caacccaaga acggattgca gagatgcaga aaattaaatc atagtgttta gaacgcagtt
    32521 gaaaaactgt gttcttttct tactaataga gatattagat tgaagtttaa ttatgagaaa
    32581 aacgctttct ttatttaaag agttatgtta caatactatt acaatcatcg tacaaatagt
    32641 gttattttga ggggcatatg agaatgattt tagcacacta tgactgtaaa aaagataaaa
    32701 agcaatcttt agatgagcat ttatggcatg tggcctgttc tagtcgacag gaagcatcta
    32761 taattggtca aggagatgtg ctttttttaa ttggtcttta ccacgacctg ggcaaagctg
    32821 atcgaacctt tcaagataaa ttattaaata atccaaatcg gcatgttgat cactcttatg
    32881 caggggcaaa atacttatgt tctattattg ggcctcatct aaaaaaccga ggggttgata
    32941 aaaatgagag aatgacattc aacgaaatgg tggggtatgt catctctgct catcatggga
    33001 tgtatgattt atgctactat tttgacgatg ctgaatatta tggctttaat aagtttaaaa
    33061 atcgtatcaa tagagactta gatggttatc actatcatga agatattaaa gggtacgctc
    33121 taaaattaga aaaaaaatta tgtgattatg gctacaaaga tttaagggag cttattgata
    33181 aagcttttga taattaccaa caagccatgt cttccttaaa ctggcaagat aagagtgagt
    33241 gggattatta tcagtcttgt atggtgagac tttacttgtc actcttaaaa aacgctgata
    33301 ttttggacac agtaaatgcc tatggcctta agataagtcc tatggataaa acagagcgat
    33361 cctttctaaa acactcctat ttagcggcca ttgaacaaaa atatgctagc tttggacagc
    33421 caaacaatca gttgaacact attcggacag aaatcgctga gcgtgttaaa gaaagaggta
    33481 aacgagattc caaggggatt tatcgcttag atttaccgac aggagctggc aagactaatc
    33541 ttagtatgcg ttatgcgttt caccaattag ttcatcacga caaatcaagg tttttttaca
    33601 taacgccctt tctttcggtt cttgagcaaa atgcttccga aattagaaaa gttacaggtg
    33661 accttggcgt tctagaacac cattccaatg tggtgaaaca ggctaatgaa gaagatgatg
    33721 acaaggacag tttattgtca gcttatctta gtgatagctg ggacagtcaa gtagtcttga
    33781 cttctatggt tcaatttttc caaacacttt tcaaaacaaa atcagctaat ctgagacgtt
    33841 tttcaagttt gattaatagt gttgtgattc tagatgaagt tcaatccctg cctattgaag
    33901 tcaccacttt gtttaattta acgatgaatt ttttaaataa agttatggat acaaccatcg
    33961 ttctttgcac agcaacacaa cctgcttatg attcttcaga gattgaccat cgtatctgtt
    34021 atggagggaa cttgggagaa ttagctgaaa tcgttgagtt aacgattgaa gaaagacaga
    34081 ttttttcaag gacagagctt agaaaatttg atgataatga tcagaaagtt cacttgactg
    34141 atgttattaa ccttattcta ggtgaggaaa actcagttct tgctattttt aatacgaaaa
    34201 aaatggttca taactgctat actatgctaa aagacatgac tgatagaccg gtctatcagc
    34261 tttcgactca atctacgact atttaagcct taatatttat cagacagcaa ataaaaagtt
    34321 caaaggtaag ttaaaacaag cttttaaaac agcaggagcc aaaatgaacc tcatcaataa
    34381 tgacatgata ggaattctcg taccttatgg cgaagctgag aaaaaattgg cttatttaga
    34441 agaattgggt atgtctcatt ttttatcagc agaagattat caaacgataa aatcattact
    34501 aaaagagtta caacctttta cggttaatgt ccgcgagaac gatcctctct ttgagacaac
    34561 aaaatcttat ctaaatggtc agattctggt tttgacgtcg gagtattatg acacggaaag
    34621 aggagttaaa tacgattcag ctagctttta cttctaactc aaaacgaaag cagattaaca
    34681 aaaggttgtt agaggacctt gttaacctgc caatcatcat taataattat tatcaattta
    34741 gactatttaa taaaattaga ttacaaaaaa acagaaggag gaaagtagct tgtacagatc
    34801 tagagacttc tacgtgagag taagtggtca gcgagctctt tttacaaatc cagcctcaaa
    34861 agggggatcg gaacggtcat cctattcggt tccgactaga caggcactga atggtatcgt
    34921 tgatgccatc tattataagc cgacctttac taatatcgtc acagaggtta aggttattaa
    34981 ccagattcaa accgaattac agggtgtcag ggctctgtta catgattata gtgcagattt
    35041 aagttatgta tcctatttga gtgatgttgt ttatctgatc aagtttcatt ttgtttggaa
    35101 tgaagataga aaagatttga actcagatag acttccagct aaacatgaag ccattatgga
    35161 gcgttctatt cgtaaagggg gacgtcgaga tgtgtttttg ggtacaagag aatgtttagg
    35221 gcttgtagat gatatcagcc aagaagagta tgagactact gtgtcgtatt ataatggtgt
    35281 caatatcgac ttgggaatca tgttccattc ctttgcctat ccgaaggaca aaaagacacc
    35341 attaaaatca tactttacaa agactgtgat gaaaaatgga gtcattacgt ttaaagcaca
    35401 gtctgaatgc gatattgtta acacgctttc tagttatgct tttaaagcac cagaggagat
    35461 aaaatcggtt aacgatgaat gcatggagta tgatgccatg gagaaaggag aaaactgatg
    35521 gattttttta cttctctctt gaagacttat gaaaaagcag agctagcaga cttggttgat
    35581 catcaaaaaa gaagtaatga gccggtttta ctgccgattt atcatacgag tttaaagtca
    35641 aatggcaaaa atatcatttc agtgaaactt gacaaagatg gccagtttca caaggcagaa
    35701 tttatggcag ataagcaaat gattattttt cctgtaacgg ctgattctgt tgctaggtca
    35761 ggtagtcatc ctgcaccgca tcccctagtc gataaatttg cttattatag tgctgaaatg
    35821 gggcagtccc agtatgattc ttttcataag caacttaata actggattga ttattgtgag
    35881 gagggtgacg tcaagaggtt tttaaccatt gttcagcaat ttattttgaa accagaattt
    35941 ctgacattga ttcttgattc tttaattgct cctgattatc aacataatca attaaaagtc
    36001 atctattttg atgctactgg aaaagaaaaa ctaattgatt tatcagcttg ctttttagaa
    36061 ttttcaattg accaatttca gggctttaaa aatgaatcgg tttcgacgtt taaagcttta
    36121 caccaatcct atatttcttt tgttgaagcc aatcgtgaaa atctcggtat ttgtaatatt
    36181 agtggacgag aggaacagct taccgataag catagaggtt tgatggggaa tgctaaaatc
    36241 atctctgtta gtaataaaag agaagcttat aaaggacgtt ttagagaacg cgaagacgtt
    36301 tttagtgttg gctatgaaac ttccgaaaag attcatttaa tgatcaagta tcttttagaa
    36361 aataaaaata ccagtacttg gttagggtct tctcaatatt taatcaactg gttcagcgat
    36421 gatttaacaa atgatagtcg gttggatatt gtatcaccaa tctttgatga tggacttgaa
    36481 gaagatgatg atgacgatac gcctcctgtt ataacattag caactgaaga caataaaaga
    36541 attggtaaat cattcatcaa gggacaaaaa ttatttgcta atgatgccac ttactacgtt
    36601 gctattttga ataaaaccag caatgggcgg attgctttaa aatattttcg tcagcttcaa
    36661 gcgtcccaat tactcaccaa tcttaacaag tggcaggaaa catacagttg ggagtcgcga
    36721 tctaagtttg gaaaaagtcg cttaagaacc cctacttttc atgacatcct taatgtgtcc
    36781 tacggggttg atagggatcg cttccttgaa ttagataatg ataacttcaa aagtgatcaa
    36841 attcaaaagt tagtggcaag tttgattgat ggtaaaccga tgccacagtc cattgtcaaa
    36901 aagttaggta acaatgttaa agaacgacat cgttaccgta agcactggta tcaagttgag
    36961 caggtctgct tagcaatttt acacaaacaa aatggggagg aattttcacc gatgctagat
    37021 cataccaatc aaaatcgttc ctatcttttt ggacgattat tagcaatttt tgaattaatc
    37081 gagaccttgc gttatggctt ggatggaaac aataacgacc gtattaccaa tgctgaacgt
    37141 tattggacag cctatactgg acaaccaaca aaattgatga tgttattgga aaataaaatt
    37201 aagccttacg aagaaccatt gaaattaaat cgtcgtggca gttggatgaa attagaaaaa
    37261 gaaaaagaag agattttaga actgttaaat cctctgttag aaacagaaac aatggaaaaa
    37321 cccttagatt accgctttat ttttgggtat tatgctgaga aaaactatta ctatacaaaa
    37381 caaaacactg aagtaacaga aagtgaggag taaaaagatg ttggaacaca aaattgattt
    37441 tatggtaact cttgaagtga aagaagcaaa tgcaaatggt gatcccttaa atggaaacat
    37501 gcctcgtaca gatgccaaag gatatggtgt gatgagtgat gtctccatca aacgtaagat
    37561 tcgtaatcgt ttgcaagata tggggaagtc tatttttgtg caagctaatg aacgtattga
    37621 agatgacttt cgttcactag aaagacgctt ttcacaacat tttacagcta agacacctga
    37681 caaagaaatt gaagaaaaag caaatgcatt atggtttgat gttcgtgctt ttggacaagt
    37741 ttttacttat ctgaaaaaat caattggggt gcgtggacca gtttccatca gtatggctaa
    37801 gtccttggag ccaattgtca tttccagcct tcaaattacg cgtagtacca atggtatgga
    37861 agctaagaat aatagtggcc gctcttctga tacgatgggg acaaaacatt ttgtagatta
    37921 tggtgtgtat gtacttgaag gttctatcaa tgcttatttt gctgaaaaga ctggtttttc
    37981 tcaggaagat gctgaggcta ttaaagaagt tctggttagc ttgtgtgaaa atgatgcgtc
    38041 gtctgcacgt ccggaaggct ctatgcgagt ttgtgaagtc ttttggttta cgcattcaag
    38101 caaactgggg aatgtttcaa gtgcgcgtgt ctttgacttg ttagagtatc atcaatcaat
    38161 agaagaaaaa agcacttatg acgcttatca gattcatcta aatcaagaaa aattggctaa
    38221 atatgaagcg aaagggttaa cggttgaaat cctagaagga ctctagtatg gtctatgccg
    38281 aagatgatta tttaatgcta tcaggtattc agcatttcca attttgtaaa cgtcaatggg
    38341 cgttgatcca tattgagcaa caatggcttg ataatgaagc gacagcgcat ggacaggttt
    38401 tacatactaa agcagataac ccttacatta aagaaaaacg aaaagagctt ttggtctcac
    38461 gtgctatgcc catttcttct gcagaacttg gactttcagg aattatggat gttgtggaat
    38521 tttataaaga tgatcaaggt gtgtctttga ggggaaaacg tgggaaatgg ttaccaaaag
    38581 ttgtggaata caagcgcgga aaacctaaaa aagataccag agatattgtc cagttggtgg
    38641 ctcagaccat gtgtttagaa gaaacgctag actgcgacat taacgaaggt tgtctttatt
    38701 accatagtgt caatcaaaga gtgattgttc ctatgacatc agctttgcgt caagaagtga
    38761 aggaattagc cgcagagatg caagaggttt atcagagtca aatgctacct aaagcagctt
    38821 attttaaaaa ctgtcagctt tgttctttag tcgatatttg taagcccagg ttgagtaaaa
    38881 aaacaaggag tgtgtcgcgt tacatcaatg aggctatgac cagtgaggag atggacctat
    38941 gaaaaagttg ctaaatacct tgtatttgac gcaagaagat ttttatgtca ctaaagaggg
    39001 cgataacatt gttatcaagc aagaaggtaa ggttctcaaa cggtttccgt ttcggattat
    39061 tgacggtatt gtctgttttt cttatttggg tgtgtcgcct gctttggtga agttatgtac
    39121 ggagaatcag attaatttat cgtttcatac accacaaggg cgtttttgtg gtcgctatat
    39181 tggttcaacc aatgggaatg tgttgttgcg tagagaacat tatcgtttat ctgatcgtga
    39241 ggaatctttg gaatacgcaa agcggtttat tttggctaaa atttccaact caaggaaata
    39301 cttgctacgc tttaaacgag atcatcgtca acagattgat accaagcttt ttgaggctgt
    39361 taatgacgaa ttgatatggg ctttagagat ggttcaggca gcagataata aagactcttt
    39421 aagagggatt gaaggccaag ctgctaatca gtattttcgc atatttaatg acctggtgtt
    39481 gacggacaaa aaaacgtttt acttccaagg tcggagtaaa cgaccaccct tagattgtgt
    39541 taatgccctc ttgtcttttg gttacagttt actgaccttt gaatgtcaat ctgccttgga
    39601 agctgtcgga ttagacagtt acgttggttt ctttcacacg gatcgtcctg ggtgtgctag
    39661 tttagcgctt gatttagttg aagagttccg ctcatatatt gtagatcgtt ttgtcttttc
    39721 attaattaat aaaggacaac ttcagaaaaa acactttgag gttaaagaaa atggtagtat
    39781 tttaatgacg gaaaatggca gagctatttt tattgatttg tggcagaagc gtaagcatac
    39841 tgaggtagaa catcctttta caaaagagaa agtaaaactt atgttattac cctatgtaca
    39901 agcgcagctt ttagctaagg ccatacgagg agatttagaa agctatccac cttttatggt
    39961 ttaggagatg ttatatgatg gttttagtca cttatgatgt aaatacggaa acacctgctg
    40021 gtagaaaaag attgcgtcat gttgccaaac tctgtgtgga ctatgggcaa cgtgttcaaa
    40081 attctgtttt tgaatgttct gtgacacccg cagaatttgt ggatataaag caccgcttaa
    40141 cacaaatcat tgatgagaaa actgatagta ttcgctttta tttattgggg aaaaattggc
    40201 agaggcgtgt ggaaacactt ggtcgctcag acagctatga cccagataaa ggtgtcttat
    40261 tattgtaaaa atctcttgtg cgaagctagc tttcacagaa acaccttgct tgctcgcgca
    40321 aaaataactt aaaaaagaag cgaaatggag ataaaaaggc ttaaaacacc tctatgtgtg
    40381 ccatccgttt ctctataaac tgtgtcattt ggcgcagtct cacccttcat gggtgagtgg
    40441 attgaaatta ctagcgttga gatagccggt attaatctta ccgtctcacc cttcatgggt
    40501 gagtggattg aaatatttcg tcacctcctc aatcaataat agagtcagtc tcacccttca
    40561 tgggtgagtg gattgaaatc taagatgata ccagttacaa taccgatttt aagcgtctca
    40621 cccttcatgg gtgagtggat tgaaataaat aattacatga ctagccaata cacccacata
    40681 gtctcaccct ttatgggtga gtggattgaa atttcatgag cttctttact ctcaaagtaa
    40741 gagtggtctc acccttcatg ggtgagtgga ttgaaagatg aacccgatct ctcttttaat
    40801 gaggtcgggt ttttacatgc ctttagtcaa ccatttatct gatagcgtat caaattcaag
    40861 tttttgcatg ttctagtcta tggtttgcgc cactagcttt cggtaaacta agggtaactt
    40921 aagagaggag ttatttatga cactatcact tgtattaatg gtatcggttt ttggggcagg
    40981 cttgttatcc tttttctctc cgtgtatctt tccggtcctt cctgtttatt taggcatttt
    41041 actggatgca gatgattcaa agacgattac tatatttgat aaaaaactct attggtatgg
    41101 cattgtcaaa accttagcct ttatttttgg tctatctact atttttgtga ttttaggtta
    41161 cggggctggt tttttaggaa atatccttta tgcggtatgg tttcggtact tactgggagc
    41221 cttggtcatt atcttgggga ttcatcagat gggcctcatt accattaaga gcttacaatt
    41281 tcaaaaatca ctgactttcc ataataacaa gaatcgcaac ggtttgttca atgcatttat
    41341 tcttggttta acctttagct ttggttggac gccttgtgtg ggacctgttt tgagttctgt
    41401 tctagctttg gtggcttcag ggggaaatgg tgcctggcaa ggtggcgttt tgatgattat
    41461 ttatactctt ggattgggca ttcctttcct actcatctct tttgcgtcag gcattgtttt
    41521 gaaacagttt aacaagctca aaccccacat gcttttactg aaaaaagtag gaggtgttct
    41581 gattatagtc atgggaatct tgcttatgac aggaacctta aataacttag cacaactttt
    41641 tggataaagg agaaacacaa tgaaaaaagg actattagta acaactggtt tggcttgtct
    41701 cgggctacta actgcttgct caacccaaga caatatggct aaaaaggaaa taactcagga
    41761 caagatgagc atggcagcta aaaagaaaga tgagatgtca acatcaaagg acaagtccat
    41821 gatggcagat aaaacatctg ataagaaaat gaccaatgat ggtcctatgg cgcctgattt
    41881 cgaactcaaa ggcatcgacg gtaaaactta tcgcttatca gagttcaaag gtaaaaaagt
    41941 ttatttgaaa ttttgggcct cttggtgttc aatctgtcta tcaaccttgg cagataccga
    42001 agacctggct aaaatgtcag ataaagacta tgtggtccta acagttgtct cgccaggtca
    42061 tcaaggggaa aaatcagaag cagactttac aaaatggttc caaggaacag actataagga
    42121 cttaccagtc ttgttagatc cggatggcaa gctattggaa gcttacggcg tcagatctta
    42181 cccaacagaa gttttcattg gaagtgatgg tgttcttgct aaaaaacaca ttggttatgc
    42241 caaaaaatca gacatcaaaa agacccttaa aggtatacat taggaaggac cagctgatag
    42301 gcattagaaa aaaggagttg agtttatgga acacaaatgg aaattatggc tatttatcgt
    42361 tggagtggtc ttgttaatag gcggagcttc tgttctcatt cagaaaggtc aagccaaggc
    42421 ttttagtcaa ggcaagcctc aactaccagc taaaaaagtc aaatccacta aagtagatcc
    42481 caatgcccct gttgatcaaa aaatgattta cctagctggt ggctgtttct ggggcgttga
    42541 agaatatttt tcacaagtgg atggcgtctt agatgcggtg tcaggatatg ccaatggtag
    42601 aggcgatacc actaattacc agttaattca tcagactggt catgcagaaa ccgttgaagt
    42661 ggcctatgat gcgaaccgta ttagcttgaa agaattatta ctgcattttt tccgaattat
    42721 tgacccaacc agtcttaata agcaaggaaa tgatcgcggt agtcaatacc gaactgggat
    42781 ttactatact gacaaagcag atttagctat tattgatgag gtcttcaaag aaaaagctaa
    42841 ggattacaag aaaaaaatag tggttgaaaa ggcgccattg aaacacttta ttaaggccga
    42901 agagtaccac caagattacc tcaagaaaaa tccaaatggc tactgccata ttgacatcaa
    42961 ccaagcgacc tatcctgtga ttgatgaaag caagtacccc aaaccaagtg ccactgagat
    43021 caaagcaaaa ctatctgcag acgaataccg agtgacgcaa aaaaacgaaa ctgaaaaagc
    43081 tttttctaat cgctactggg attcttttga tgcaggaatt tacgtagacg ttgtgacagg
    43141 agaaccgctt ttctcttcaa aagacaaatt tgaatcaggt tgtggttggc caagctttag
    43201 ccgccccctc agcccagacg ttgtccgcta caaggaagat aagagtttca atatgacgag
    43261 aacagaagtg cgcagccgtt cagggaattc tcatctgggg catgccttta ctgacggacc
    43321 taaagaccaa ggcggccttc gttactgcat caatagcttg tctattacct ttattccaaa
    43381 agctgatatg gaagctaagg gctatggtta tttattatca tctgtagaat agggttaggc
    43441 ctaaaaatct gatataatag aagaaagact agtatgagag gaccatgcaa ggccctttat
    43501 aaagataagg agacaccgtg tgtactcatt gttaattgta gaagacgaat accttatccg
    43561 ccaaggtatt cgttctttgg ttgattttag ccagttcaag attgatcggg tcaacgaagc
    43621 agaaaatggc cagttggctt gggacttgtt tcagaaagag ccttatgata ttgttttgac
    43681 ggatatcaat atgcccaaat taaatgggat tcaactggcg gagttgatta aagagcatgc
    43741 gcctcagacc catttggttt ttttaaccgg ttatgatgac tttaactatg ctttgtctgc
    43801 tttgaagtta ggggcagatg attacttgct caaacccttt tccaaggccg atgtagaaga
    43861 catgttagga aagctccgga aaaaattaga cctttccaag aaaacagaaa ccattcagga
    43921 attggttgag cagcctcaaa aagaagtatc agcaatagca atggctattc atgagcagtt
    43981 ggcagattct gatttgaccc taaaaagctt ggctcagcag cttggtttta gccctaatta
    44041 ccttagcgtc ttgattaaaa aagagttagg gatgcccttt caagattatc tggtacaaga
    44101 acggttgaaa aaagccaagc ttctcttgtt aaccagtaat cttaaaatct atgaaattgc
    44161 agagcaggtc ggttttgagg atatgaatta tttttctcag cgctttaagc aactggtagg
    44221 cgttacccca agtcagtata aaaaaggagg ccaggcatga agcgttatcc cctactggtc
    44281 cagttgattt cttatgtctt tgtgatcgta attgccctca ttaccacgct tggtttgctt
    44341 tattaccaga cgagttctcg taatatcagg caattaattg aacgtgatac ccggcagagc
    44401 attcggcaaa gttcccaatt tattgacgct tacatcaagc ctcttaaaga aacaatttcg
    44461 gtgctggcga aaaattcgga aattaaagac tttgctagtc aaattcatca agaaaatgac
    44521 aaacaggttt ttcagctcat gaagatggtt cttgcgacca attccgattt acaagcagct
    44581 gttctggtga ctaaggatgg ccgaaccgtt tctaccaatc ctcagttgat tatgaaaacc
    44641 tccagtgata tgatggcaga accctggtat aaagcagcca tagaccgtca agccatgcca
    44701 atcttaaccc cagctcggca attatccctt tcttctaaaa aagaatgggt agtttctgtg
    44761 acccaagagg tagtggatgc tgttggtcat aatttaggcg tgctaagact tgatattgca
    44821 tacccgacca ttaaagcgtc tttagatcag cttcagctag gccgccaagg ctttgccttt
    44881 attgtgaatg ataagcatga atttgtttac catcccaaaa agagtgttta cagttcttct
    44941 aaggagatgg ctgcgatgaa gccttattta gcgattcaga atggttacac taaggacaag
    45001 acatcttttg tttaccaaaa actcattcct aacagtcaat ggactttagt gggagtggcg
    45061 tcactggatc agttgcaccg ggtgcagcgc caaatttttt ggtccttcct tggaacaggg
    45121 cttctagcct gtctgatttg tagctttgca actgtcttag ccttacgcag atggattcgt
    45181 cccattcaac aattgcagca agttattctt gctattcaaa aaggagatcg tcagttacgt
    45241 gcccaagaaa cgggttctcc agaattgaca gaccttgtcc aacagtttaa tgcgctctta
    45301 gatcaaattg atagcttgat ggttgccgtt gcggataagg aaaaggcgat tgggcagtat
    45361 aggttacaag ccttggctag tcagattaac ccgcattttc tctataacac cttggacact
    45421 attatttgga tggcagaatt taatgacagc aagcgcgtgg tagaagtgac caagtctcta
    45481 gctaagtatt ttcgtttagc ccttaatcag ggaaacgaat acattcgttt ggcagatgaa
    45541 ctggatcacg ttagccaata cctctttatt caaaaacagc gctatggaga caagctaagt
    45601 tatgaagtgc aaggcttaga tgtctacgca gactttgtta ttcctaagct tatcttacag
    45661 cccttagtag aaaatgctat ctaccatggc atcaaagaag tcgatcgcaa gggcatgatc
    45721 aaggttacgg tatctgatac agctcagcat ctgatgttga ctgtttggga taatggtaaa
    45781 ggcattgaag actcttcact gaccaatagt cagagcttgt tggctagggg aggtgtgggc
    45841 cttaaaaatg ttgaccagcg gttaaaactt cactatggtg aaggctacca catgaccatt
    45901 catagccagt cagaccagtt cactgaaata caattaagcc ttcctaaaat gcatgaatta
    45961 atggcagacg acacacagga aaacgagtaa ggttacagga agtgcatgtc agccatttcc
    46021 ctaagttagg gtattggaga taagaacctt agaaaggata tcatgagttt agcatctgac
    46081 tattttagcc gacagacccc catagttgaa aagctaatgg cttacggttt tgaaaaacga
    46141 aataatggtt acttctataa cgaacggttt atggaagaag aatttgaggc gcagcttagg
    46201 attgatgagg ctggcaatat ctgggatcgg gttattgatt gtgacttgga ggaggattat
    46261 ttgcctttac aacaagcagc ctggcaagga acctatactg ggcaggttag agcggcctat
    46321 ttggaacttt tggagcgctt atcagtagcc tgttttgaag ccactccttt tcagtcaatg
    46381 caagctaata gacttgctaa gcatatcacc aaagaatggt ctgacccaat ggactatcct
    46441 tttgaaaagc acccagacct tgcaacctat cgggtagggg gcaaatggta tgctatgatt
    46501 ttttctctct tggctgataa attagaccag ataccagaga gattagtagg acagacatgt
    46561 gaagtgatga ctgtcaaagt taatcccaag gatttacctc agttactgca gcaagaaggc
    46621 atttacccag cgtatcacat gtcaaagaaa aactggatta gtattatttt ggatgataag
    46681 ataacagatg ataagctttg gacgttagtg actcaaagtc gtcaactagt caacccaaac
    46741 ggtctttcta atcctaatgg tcctgattat tgggtcattc cagctaattt gaaatactat
    46801 gatattgatg cagaatttgc agccaatgat gttattttat ggacccaaaa agctggtatt
    46861 gccgtgggag actacgtgtt gatttatata acagccccag tcaaatccat tcgctatgtc
    46921 tgccaagttc tagaaactga tatacctaac gagggttatc gtaaaaatcc taacattgac
    46981 aaactcatgc gtctccgaaa atgccaacag tacaaggatg gtctcttgtc ctttgacctt
    47041 atgaaagaac atggcgtagc tgcagtgaga gggccaagac gattaagccc tcaattaatt
    47101 gcatttttaa aggaaaaaga gtattttaaa gagaacaact aaaggagaat aagagatggt
    47161 tgaatttgta gcagatcaaa gtttttggga actttttcca gatgccaaac taggtgtcgt
    47221 cttattaaag gatgttcaga accaagcaga gtcaccagaa gatgtcaaac agctcttagc
    47281 agacagccat gagttagcaa aagcttattt aacagcagac aattttagtg ataatcaggt
    47341 ggttcaagtc taccgcaagg cctatcaaca ctttaaaacc aaaaaaggag ctcgctctag
    47401 cattgaagcc cttctcaagc gggtgtcaaa tggtcaaagc attccgtcta ttaacccact
    47461 agtggatatt tataatgcgg ctagtctacg ttttggttta cctgctggag ccgaagatag
    47521 tgacagtttt atcggagatt tgcgtctgac gataacagat ggtggtgacg acttttactt
    47581 gattggggat gctgacaaca actcaacctt gccaaatgaa ctctgttata aagatgatat
    47641 tggagcggtt tgtcgttgcc ttaactggcg agatggcgaa cgcaccatgg tgacagaaca
    47701 caccaaaaat gcatttctga ttatcgaagc tcttgatcaa gaaggtcaaa atagattgca
    47761 agaagctctg aaattcattg aaggcagcgc aaaaatgtac cttcatgcaa tcacttctgt
    47821 gcatgtgttg gataaagaca atccacacgt gccacttttt acagaaaact agattagtag
    47881 gatgagctgt gtaaaataga aggttaacaa aaaaagcgag taaacgaatg tcctgtcatt
    47941 taggttttcg tttactcgct ttttgatgag attttactgc tgtcctagca aaaggtgagg
    48001 atgaggtcgt atatcacata gaggttttct gttaagcctt atttgttact aaagaagaat
    48061 ggtggtgtgt aatgactaag agtctcgtaa caagccttgg cttgctcatt gtcttcacag
    48121 acaatcaagc aagtatcatc accacagatc gttgctacaa tttctggaat ttgcatggca
    48181 tctaaaatgg agccaaagga ttgggctagt ccaggtaacg tttttaaaat aatttgatgt
    48241 tgaactatct ttaacatgac caaagcgtct tccatgtaaa aacgaagacg atgttcccag
    48301 cgggtttgtg aaatggctaa cgcttcatag tgtgtgtcgt ttccagaagt tactttgacc
    48361 aggttcaatt ccttcatgtc acgtgatagg gtagcctgag tgatcgtgat gccatttttt
    48421 tgcaaccgtt cttgtaattc ttgctgtgta tgtatagttg tctcagagat gagggaacgt
    48481 atgagttggt gacgcgtctc tttcttattc attgttataa tcttccttta acgtaattga
    48541 taacaatccg agtatagcaa aaagaggaga aggtgtaaag tttttgatga accgcttaca
    48601 aaatggcttt tttatggtaa aataaagatg atgtttagaa aaaggagatt tgtgtgattc
    48661 gaagagaaga ttatcaatac cttagaaaac taaacgattt tcgctacttt tctattgaac
    48721 aatttgacaa aattgtaggg cagatggaat tccgaaaagc taaaaaggac catatccttt
    48781 tttttgaggg agataaaaga gataagcttt ttctagtgac ctctggttat ttcaaggtgg
    48841 agcagtccga tcaatcagga accttcatgt atacggattt catcagacac ggcaccattt
    48901 ttccttacgg tggccttttt acagatgatt attaccattt ttcagtggta gccatgacag
    48961 acgtgactta tttttacttt ccagtcgatt tatttgaaga ttactcgctt gaaaatcgtc
    49021 tacaaatgaa acacttatat agtaagatgt ctaaactgct cgagttacat gagttgaggg
    49081 tccgaaactt gattacatca agtgctagtt ctcgtgtgat tcaatctctg gcgattttgc
    49141 tggttgaaat gggtaaagac agtgatacct taccttttca attaacaaca acagatattg
    49201 ctcaaataag tgggacgaca agagaaacag ttagccatgt gcttagggat ttgaagaagc
    49261 aagaattgat taccataaaa ggtaagtatt tgacttatc
//
LOCUS       DAUELZ010000002        46401 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.002, whole genome shotgun sequence.
ACCESSION   DAUELZ010000002 DAUELZ010000000
VERSION     DAUELZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46401
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.002"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(14..757)
                     /locus_tag="VHI09_000044"
     CDS             complement(14..757)
                     /locus_tag="VHI09_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ6119958.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            complement(757..1560)
                     /locus_tag="VHI09_000045"
     CDS             complement(757..1560)
                     /locus_tag="VHI09_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ6119959.1"
                     /translation="MTSVFLTSGWAFYDHLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKFLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            complement(1754..3097)
                     /locus_tag="VHI09_000046"
     CDS             complement(1754..3097)
                     /locus_tag="VHI09_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ6119960.1"
                     /translation="MSFKDYHFKQYVQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDEAKAEVQVVITAPSRELATQIFDACKQIAKHSQEEIRL
                     ANYVGGTDKLRQIEKLKGSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERKAKKQHF"
     gene            complement(3255..4265)
                     /locus_tag="VHI09_000047"
     CDS             complement(3255..4265)
                     /locus_tag="VHI09_000047"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEQ6119961.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLAGGLMFYFLHVSPSGISSINVFGYQLSLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQGQFDVLLLIGTMIGALLGFFC
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            complement(4267..6522)
                     /locus_tag="VHI09_000048"
     CDS             complement(4267..6522)
                     /locus_tag="VHI09_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ6119962.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            complement(6526..6849)
                     /gene="ftsL"
                     /locus_tag="VHI09_000049"
     CDS             complement(6526..6849)
                     /gene="ftsL"
                     /locus_tag="VHI09_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEQ6119963.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            complement(6854..7867)
                     /gene="rsmH"
                     /locus_tag="VHI09_000050"
     CDS             complement(6854..7867)
                     /gene="rsmH"
                     /locus_tag="VHI09_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEQ6119964.1"
                     /translation="MKPFSLTFSFVNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     NGIYVDATLGGSGHSAYLLSKLSEQGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPTFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            complement(8158..8331)
                     /locus_tag="VHI09_000051"
     CDS             complement(8158..8331)
                     /locus_tag="VHI09_000051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6119965.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFVFFFFGD
                     GMLVSWIKRRNNE"
     gene            complement(8351..9601)
                     /locus_tag="VHI09_000052"
     CDS             complement(8351..9601)
                     /locus_tag="VHI09_000052"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEQ6119966.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKVLVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALYSNKALVKL
                     IRQSLEESGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGARLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATKIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            complement(9594..10415)
                     /gene="proB"
                     /locus_tag="VHI09_000053"
     CDS             complement(9594..10415)
                     /gene="proB"
                     /locus_tag="VHI09_000053"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEQ6119967.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVITQEIIASAGGAGSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVRHD"
     gene            complement(10479..12107)
                     /locus_tag="VHI09_000054"
     CDS             complement(10479..12107)
                     /locus_tag="VHI09_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6119968.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQEKHPKENFKAYKSMM
                     RQQALMIAMFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVIQAPFSTAALLNFWLPLLLVLAMVYGIVTKVMPNYYR
                     EAFYISNENKAKQTKKPVNRPHQNQSLAQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGIMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     EFMYRRDYRLLDLKWQDMTQLFTRGNGQWLTMGLIFGNLIVTGVVGFGAVIIANIIQQ
                     PLLISVLLSCLTLMVLGIAHLWIQKTFWQSLEKL"
     gene            complement(12112..12846)
                     /locus_tag="VHI09_000055"
     CDS             complement(12112..12846)
                     /locus_tag="VHI09_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6119969.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKGMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            complement(12880..13146)
                     /locus_tag="VHI09_000056"
     CDS             complement(12880..13146)
                     /locus_tag="VHI09_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ6119970.1"
                     /translation="MEKKRQRLYDVIRHAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            complement(13338..15323)
                     /gene="tkt"
                     /locus_tag="VHI09_000057"
     CDS             complement(13338..15323)
                     /gene="tkt"
                     /locus_tag="VHI09_000057"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEQ6119971.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLTVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVDHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAAYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQSGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNTAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLDGIIIATGSEVKLAMDT
                     QEALEAEGIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            complement(15541..16185)
                     /gene="fsa"
                     /locus_tag="VHI09_000058"
     CDS             complement(15541..16185)
                     /gene="fsa"
                     /locus_tag="VHI09_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ6119972.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            complement(16307..17806)
                     /locus_tag="VHI09_000059"
     CDS             complement(16307..17806)
                     /locus_tag="VHI09_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ6119973.1"
                     /translation="MKIEDLMDKERRAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYFEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYTLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMGQETDFGQQLSDDILALLVFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVYLPRQDLP
                     YYRLKQYSSPYELAALEDFLKSLFQQKNKQEEEPLVSPKAKSSFEHNSV"
     gene            complement(17796..19142)
                     /locus_tag="VHI09_000060"
     CDS             complement(17796..19142)
                     /locus_tag="VHI09_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ6119974.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNHRFIQAEVLAIEAPSNTLFLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSIHHVTFINNKHVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QANIYAIGDMVSLHFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGDSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDADLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            complement(19251..19952)
                     /locus_tag="VHI09_000061"
     CDS             complement(19251..19952)
                     /locus_tag="VHI09_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ6119975.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVSGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMLGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            complement(19954..21792)
                     /gene="glpO"
                     /locus_tag="VHI09_000062"
     CDS             complement(19954..21792)
                     /gene="glpO"
                     /locus_tag="VHI09_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEQ6119976.1"
                     /translation="MEFSRETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDEIRQFSHKGRPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQI
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVINEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGHQEG"
     gene            complement(21808..23334)
                     /gene="glpK"
                     /locus_tag="VHI09_000063"
     CDS             complement(21808..23334)
                     /gene="glpK"
                     /locus_tag="VHI09_000063"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEQ6119977.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKEDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGIGGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQFA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            complement(23694..24086)
                     /locus_tag="VHI09_000064"
     CDS             complement(23694..24086)
                     /locus_tag="VHI09_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEQ6119978.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            complement(24213..24470)
                     /locus_tag="VHI09_000065"
     CDS             complement(24213..24470)
                     /locus_tag="VHI09_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEQ6119979.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            complement(24624..26663)
                     /locus_tag="VHI09_000066"
     CDS             complement(24624..26663)
                     /locus_tag="VHI09_000066"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEQ6119980.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKER
                     QEMIVEQIKAIEAAQGVQVDIDADLLNEVLNLVEFPTAFMGSFDAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQEGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYARLAGEDAAVATAIREHYLPDAAGGALPETKVGAV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYANKADVMNFIRARVDKMMGKAVPKDIREAVLESSTFVVPEMLAAAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRLAILSDLVSKAKAIAAFNQLNTK"
     gene            complement(27041..27958)
                     /gene="glyQ"
                     /locus_tag="VHI09_000067"
     CDS             complement(27041..27958)
                     /gene="glyQ"
                     /locus_tag="VHI09_000067"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEQ6119981.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEMSDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDDE"
     gene            complement(28330..28863)
                     /locus_tag="VHI09_000068"
     CDS             complement(28330..28863)
                     /locus_tag="VHI09_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEQ6119982.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KNHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            complement(28995..29834)
                     /locus_tag="VHI09_000069"
     CDS             complement(28995..29834)
                     /locus_tag="VHI09_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEQ6119983.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASIDRLGLDYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTQEDKKTIRYLSGMSAIPNPDT
                     TSF"
     gene            complement(29956..31104)
                     /gene="nagA"
                     /locus_tag="VHI09_000070"
     CDS             complement(29956..31104)
                     /gene="nagA"
                     /locus_tag="VHI09_000070"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEQ6119984.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLSLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKNPRLEEFDAWQKAAK
                     GLIKKIALAPERDGVAEFVSTVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVSPVACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            complement(31222..32853)
                     /locus_tag="VHI09_000071"
     CDS             complement(31222..32853)
                     /locus_tag="VHI09_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEQ6119985.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKPFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADDKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            33055..33777
                     /locus_tag="VHI09_000072"
     CDS             33055..33777
                     /locus_tag="VHI09_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6119986.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            complement(33906..34748)
                     /locus_tag="VHI09_000073"
     CDS             complement(33906..34748)
                     /locus_tag="VHI09_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ6119987.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTLTGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYAGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            35041..35598
                     /locus_tag="VHI09_000074"
     CDS             35041..35598
                     /locus_tag="VHI09_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ6119988.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLETKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            complement(35634..36458)
                     /locus_tag="VHI09_000075"
     CDS             complement(35634..36458)
                     /locus_tag="VHI09_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ6119989.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(36460..37077)
                     /locus_tag="VHI09_000076"
     CDS             complement(36460..37077)
                     /locus_tag="VHI09_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEQ6119990.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLMARGWVRAAVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            complement(37274..38251)
                     /gene="lacD"
                     /locus_tag="VHI09_000077"
     CDS             complement(37274..38251)
                     /gene="lacD"
                     /locus_tag="VHI09_000077"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ6119991.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVKANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMLVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EEEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            complement(38298..38751)
                     /locus_tag="VHI09_000078"
                     /pseudo
     CDS             complement(38298..38751)
                     /locus_tag="VHI09_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            complement(38761..39276)
                     /gene="lacB"
                     /locus_tag="VHI09_000079"
     CDS             complement(38761..39276)
                     /gene="lacB"
                     /locus_tag="VHI09_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ6119992.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTYDHTRTHYPI
                     FGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEELNAN
                     VIGFGGKITGELLMCDIIDAFIKAEYKETEENKKLIAKIAHLETHHANQEDPDFFTEF
                     LEKWDRGEYHD"
     gene            complement(39291..39716)
                     /gene="lacA"
                     /locus_tag="VHI09_000080"
     CDS             complement(39291..39716)
                     /gene="lacA"
                     /locus_tag="VHI09_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ6119993.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEESYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            complement(39973..41424)
                     /locus_tag="VHI09_000081"
     CDS             complement(39973..41424)
                     /locus_tag="VHI09_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEQ6119994.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAARNHLPVFVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            complement(41450..41755)
                     /locus_tag="VHI09_000082"
     CDS             complement(41450..41755)
                     /locus_tag="VHI09_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ6119995.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIESQMTDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFEMPIPTVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            complement(41748..42221)
                     /locus_tag="VHI09_000083"
     CDS             complement(41748..42221)
                     /locus_tag="VHI09_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ6119996.1"
                     /translation="MFPNILFTEARTQPELFDLVASHLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDFKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            42458..43228
                     /locus_tag="VHI09_000084"
     CDS             42458..43228
                     /locus_tag="VHI09_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ6119997.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQFTQLLVAHENKE"
     gene            complement(43432..43635)
                     /locus_tag="VHI09_000085"
     CDS             complement(43432..43635)
                     /locus_tag="VHI09_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEQ6119998.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGEAKVT
                     GRPLTFLLKRALKDTKFELHKWS"
     gene            complement(43649..45880)
                     /locus_tag="VHI09_000086"
     CDS             complement(43649..45880)
                     /locus_tag="VHI09_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEQ6119999.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKTVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPSFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGYP
                     NMDSLVALATSFAFAYSLYGVVEISMGQAHFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTSQAIQKLLSLKATVVRVWRNSQWQLLPLEEVSYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESFPVEKGEKDNVFAGSLNGKGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADRVSGVFVPVVLVLTLLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQKLLT
                     FSGDEHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNATLMATLGIDLASVQSVCAKASGKGQTIVYYAKEGQLRALFSIADAVKEDS
                     QATVEALHQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVITNLQSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKALSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            complement(45880..46317)
                     /locus_tag="VHI09_000087"
     CDS             complement(45880..46317)
                     /locus_tag="VHI09_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEQ6120000.1"
                     /translation="MNQNISDAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKT
                     LIGRLVKKNLLTSYRQGRAYIYQALLDETLLQKEALATVLDGICQRQHTRLLLERLYD
                     LPMTLEEIEAFQELLEVKKENAVLEVPCNCLPGQCHCQKKETY"
BASE COUNT        13895 a        10190 c         8410 g        13906 t
ORIGIN      
        1 caaaagaata tcattaaatg tatgtttttg aatagcttgc aaaaaattct ttggttcgtt
       61 ctttttgagg atgtctaaag acttcttcag gtgttccttg ttccaagatt ttcccttgat
      121 ctaaaaagag tactctatct gccacttggt aaacaaaatt catgtcatga ctcactaaaa
      181 ccatggtctg tccggattta gcagcatcag taatagactt ttccacttcg ccaactaatt
      241 ccgggtcaag agccgaggtc ggctcatcta gtaataaaac atctggtttc atagccagag
      301 ctcttgctaa agctacccgt tgtttttgtc cccctgataa atgacgtggg taatgatgtt
      361 ttcgatctgc taaaccaacc ttagccaatt ctgcttgcgc cagcttagtc gcttcttggt
      421 cagacagttt tttgaccacc ttaagacctt ctttgacatt ctcaagggct gtacgacgct
      481 caaaaagatt aaattgttga aaaaccatgg caagtttccg acgtaaaata aggacttgct
      541 ctgtggtaat ggtctcaaaa tcaaccgtaa agtcaccaat gctaatggat cctgaatctg
      601 gtttttccaa atagtttagg ctacgaagga aggtcgattt ccctgctcct gaagcaccaa
      661 ctaaagcaat cacttgacct ttttcaatat caagagccaa actatctaaa actttttgcc
      721 ctgagaatgt cttgctcaaa tttctaattg taatcatcaa cgcaattctc ctaacttttc
      781 atttctagct tgttctggtg ctttgatggc catcttgttt tcaatcaagc gacctacttg
      841 ctccatcaaa atactgatag accaatagac aagagcaaca gagatataac gttcaaaata
      901 acggtaatct gatccgccta gaatttgagc ctgggcaaac atttcgacaa tccctgcatt
      961 aaaggcaagc gatgttcctt tagttaagcc aattaaccca ttaatcaagg ttggaatagc
     1021 cacaacagtt gcattaggaa taatgacacg acggtaaacc tgcacagaag tcatgcctag
     1081 actttttgct gcttcaattt cacctgtatc tactgataaa atggctgccc gaatagtttc
     1141 actagcgtag gctgcttcat taaaagcaaa ggctgtaatg gcaaaaatag aagccggaat
     1201 tgcattaaca ttccaatcaa agccatactt ctgattgaga aattttagaa agaggggaat
     1261 accgtagtag gtcaacatca actgtaccaa aataggagtt cctcgcaaga aactcacaaa
     1321 aatagcttga atagggtata gtagcttcac tttattaatt ttgacaaggg caaaaatcaa
     1381 agccaaaacc aagccaaagg ttgctcctga tagtgtcaaa ccaagcgtaa ttggaagccg
     1441 ttggataata tttggaatgg catcaaaaac agcatgccaa ctaaaaagtt ttccatgtgg
     1501 aatcggtgag atgagatgat cataaaaagc ccaaccactg gttaaaaaca ctgatgtcat
     1561 gtatttcccc tttgtatttg tttgtcttaa tattctaaca ctcttagacc cacatgtaaa
     1621 atagtaaatc cgtattatag caataagcaa aagctatgat cactttttgt gttaacaaat
     1681 ggtaaacaat acaagtagca ggtgtaaaaa agcaagatta gatgccaatt gatcacttac
     1741 ttgctctttg ttcttaaaaa tgttgttttt tggccttacg ctcagcgcgg cccttagcac
     1801 gattttcagc acgacgttct ttgcgacgtt tttcatcaac cgcccactga atttttttct
     1861 tgtaacctgg tttgactttt ttctttttct ttttgaccaa accaatcatt tctgtgtcta
     1921 atttttgata ggctttttca cggttttgac ggcgatcacg gtcataggta tcttgaaatt
     1981 ctccattttt gagcaccttc ggtgtaaagg caatccccat tttttccagc tctttgatat
     2041 ctgagtcgtc acttggctgg taaagtgtaa tggcagttcc tgccatacca tttcgacctg
     2101 ttcggcctac acgatggaca aagaaagaca agtcttgtgg gatggcgtca ttgatcacat
     2161 ggctgacccc ttcaatgtca atcccacgcg ctgctaagtc tgtggcaaca atgtattcaa
     2221 agtccagttt tttgacttgg ttcatgatac gcttacgttc tcttggagga atgcccccat
     2281 gaatcttagc cacttttaag ccattagctg ttaaaaaggc atgcaaatca tctgcgcgtt
     2341 ccttggtatt gacaaaaagc atagccatat aaggctgcat agttttgaga atttctaaca
     2401 gttgaccatt cttatcacgc ccttttgtcg acacaagcca gttatcaatg gtatctgcga
     2461 taacggtctt tgtcttgatt tgttcaataa caggattggt caagtatttt ttcaaaaagg
     2521 gttgtaattt ctgtggaata gttgctgaga acaccaaaat ctgaacagat tttggaagcg
     2581 aagctgcaat cttatctacc gtatccaaaa agcccatgtc catggtcata tcggcctcat
     2641 ccacaacaaa ggtcgttgct ttatgaatcg ctaaatcacc agacttgacc aagtcataaa
     2701 tacgacctgg ggtcccgatg acaatatgcg gctggctgcc ttttagtttt tcaatctgac
     2761 ggagtttgtc tgtgcctcca acatagttgg ccaaacgaat ctcttcttgg gagtgcttag
     2821 caatctgttt acaagcatca aaaatttgag ttgctaattc acggcttggt gctgtaataa
     2881 cgacttgcac ttctgcttta gcttcatcaa gtttttcaaa aatgggcaac aaaaaggtgt
     2941 gggttttccc tgaacctgtt tttgattccc caactaagtc acgaccggag ttaacaatag
     3001 gaatcagccg cttctggacc tctgttggat tgacaaagcc aatttcttcc agcgcttgct
     3061 gaacgtattg cttaaaatga taatctttaa atgacatgtt ttacctcgtt ttcttccata
     3121 ttatatcatt attgttagag acaagcaaaa ggagtgggac aacactcaca aaatcagcat
     3181 ttgtgggtca tctccactct cttattgatg atgttaatca aaatactatt ggtgactatt
     3241 cttgataatc ccccttagaa gacgtataag atagctaaaa ctagtaaact agctagactt
     3301 cctactcccc acaaaaaagc atctacctgc cattctgacc acttcttgcc tttaccagac
     3361 aagcctccca attccaaatg gtggtggaaa ggcgtcattc taaaaatacg acgaccttct
     3421 ccatattttt tcttggtgta cttgaaatag gacacttgca acatcacaga acttgtttca
     3481 agaacataaa cgatcccaat aatcaggaga gtccattctt gacgaagcgc aatagaaata
     3541 gctgccaaca tagctccaag ggctaaacta cccacatctc ccataaatac tttggcaggc
     3601 ttgtggttaa agcagaagaa accaagtaaa gcaccaatca tggttccaat caataataag
     3661 acatcaaatt gcccctgaac gtaggcaata acgccgtaag tcactaaact aatcaccact
     3721 gagatagaag ctaagccatc gataccatcc gtcaaattaa cggcatttga gaaaccaacc
     3781 acccagaata gaacaaagaa taggtagaaa attcccagcg acagctgata accaaacaca
     3841 ttgatcgaag aaatgccact tggactaaca tgtaaaaaat agaacatcaa tcctccagcc
     3901 aactgcaagg ctaatttttg tttggctgtc aatccttcat tgatttgctt aaaaattttt
     3961 aaaaaatcat ccaaaaatcc gataatcccg taaatgacaa caattgataa aatgcctgaa
     4021 atcaaagcta aactttgagt gtttttgatc gaaaatagac taaccaataa cgatactgcc
     4081 gtagctacta aaagaaaaac agttcccccc atagtcggtg ttccagcttt agctaaatgt
     4141 tgcttgacat cttcatgcat ttgttgccca ccaatttttt tcaattgata gaatttgata
     4201 aaataaggca ttgtgaaagc agataccata aaggatatga ttgctgcaat aagggttaaa
     4261 aacatgttaa tctcctaaag taatggtcat tttttttatt tttttcaagg acttaccaac
     4321 atcaacactt tgtttcataa cacgaccaga atctgttcct ttaaagctga tgtctatccc
     4381 agtccattta gcaaaggttt taacattgga ttttgtccag ccatacatgt ctggtacctc
     4441 cacaaaacgg tctgataata tgagaacttg ttggttttct gttaacgttt gaccgggctg
     4501 atgcgatact tttttgatct tgctgccagt accaaggaca actggctgga caagatttcg
     4561 acgcaactcg cttgatgtct caccaggatt ctttcctaca aagtttggta atttataagt
     4621 tgtttgacga ttagcatctg atactactgg cttagttagt gtatcttgca ttaagtatgc
     4681 ttcttccaat actgggttaa ccacatcttg ccaaaaaagg ggaccaaaat gttgtggttt
     4741 agtcatagta acatacatca aaaagtctgg tttatcagct ggtaccattg ccacaaccga
     4801 atagacatag ttagtcaatc caccatcttg ataaccactt ccatcttctg aaccaatttg
     4861 tgctgttcct gatttaacag caacaggtaa atcacccact ttaataattg gtccaaatgt
     4921 ttttgaatag agtgttccaa actcagggtc tgttcctaca ccaatcatgt attgtcttgt
     4981 ttcactagcg gctttttttg atacaggttt tccaacaatt tctttatttg ccgttctaaa
     5041 acttgctgtg ttaggatcat aaatttgact gataaattgt ggctctaaca tctcaccatt
     5101 attagaaata gcagtaaagg ctctaagcat ttgaatctgg gttacagaaa tcccttgacc
     5161 aaaagcgctc atagcttgag tcacgatatt atcagaagga aatatacctg cgtcttcatc
     5221 ttttaagcca aaacgagtag gaaaaccaaa gcggaacttc gtcaaataat tcatccattt
     5281 tgcattaccc attttttgtt caagtttagt catcccaacg ttacttgaga aggcaaaccc
     5341 ttgtgcataa ttcatgtact gtcctgtgga aatgccttcg ttaatagacc agtcttgaat
     5401 agtagcatct gcaattgtta aaccattagc attgctaaag gtttcgtttg ggttgaaaac
     5461 tttatcatca atagccgctg ccagagtcat cactttcatg gttgagcctg gctcaaaatt
     5521 tccttgatga agtgcactgt accatttgta gttcgtattt tcaagtcctt tcagagtatc
     5581 agcattataa gtggggcgtt gtgttgttgc caaaatttca ccagttttag cattaacaag
     5641 tgttgcactg gccaactgac catttgattt ggcttgaaaa acatccatct gggtttctaa
     5701 gaaggtctgg ataggctcag atagcgttgt gtaaatatct ttgccatcaa tagctttctt
     5761 gacagtctta cctgtaccca agagtgtggt cccatttcga tctttttgat aagtaataac
     5821 accatcttga cctgataaaa ttttatcaaa agaagcttct agacctgttt ttccaactaa
     5881 actcttaaca cctgtctttt tatcttctgt tagagatgct aagcctataa attctgaagc
     5941 aaatgtccca tttggataca tacgaccagg actagttgtg aaagcaattc ccttgatttt
     6001 ggcatcttcc atagcctttt gaatagtaga catagtacta tatgaaatgc cagaccctga
     6061 cggtccaaac gagacttgga aaagtccttt acgcttaagc tgtttaatta catctgtttt
     6121 tttcattccc agatgctttt ttaaaatatc agctactgtt tcatactgag aaggttgtac
     6181 atataacttt tcatctgaag ccgagacaaa tgatttatcc aaaattgcgt atatgctata
     6241 agtcgtagaa tccactgcaa tagctgtacc atttcgatca taaatggtcc cacgcttagc
     6301 ttggatcgta acggtttctt gataaacctt cttcgcccct tctgacaaac taactccaaa
     6361 cttttgatct gttccaataa taatcatgaa attaataatg aaaataaaga aaataaagat
     6421 agttaagagc atcatatttt gtccaactcg aacgcgattt tcgactggag ttctcctatc
     6481 gcgcacaaca taatctaaaa catatttttg ccattttttc atagattact cgactttctt
     6541 gatattatta ttacggttgc ttaagcctgc tttaccagca atatctataa tacgatcacg
     6601 gcgagataac tcatttactt cttgcttggc attattaagc tctagttttt gatccgaaat
     6661 atgactattt aaactagtga tttcttgttg tagctgcaac ttacgacttt gcaggtaaat
     6721 aatacttact gccatcgtaa tcgctgttac aatgatagca gtataaaaag ccttctcaat
     6781 tctagagaaa gtttttattc gtttttgcaa ggcatttgta actgcctgag ttcttttttc
     6841 atttgtcata ggcttatttc cgaatttttt tggcaacacg tagcttggct gagtgtgccc
     6901 ttttattagc tgttaactct gaatgactag gtaagatcgg cttacgtgaa acaagttcaa
     6961 atgtcggttt catatcttca ggaattagag gaagcccttt tggcacatcc accgtactag
     7021 cttctttaaa caactgcttg gttaggcgat cttccagaga atggaaggta atgactgaga
     7081 tacgaccatc aagggctaat aattccatag cgtcctgaat agattcatca gccgctccca
     7141 attcatcatt gacttcaatg cgaatagctt gaaaaatctg tttagcagga tggccttttt
     7201 tcttcaactc tttagctggc tttgctgcct taatcaattc tgccaactct gttgttgtct
     7261 caataggctt aatagctctt gcttgttcaa tttttcgagc gatctgcttg gagaatttat
     7321 cttcaccata tttgaaaaaa atcttaacca aatcattgaa tggataggta ttcaccactt
     7381 cgtaagctgt taagagcgac tgacgatcca tgcgcatatc caatggagca tcttgtttat
     7441 aagaaaaccc tcgttccctt tcatccaatt gtgggctgga aacaccaagg tcgtataaga
     7501 taccatcaat ttcatcaact ccaagcgctg ttaaacgtgc tttgaggtgt ctaaaattat
     7561 ctttaataaa agttacctgt cctttgtcaa tataagattt gagggtaact tgtgcattgt
     7621 caatagcctt ttgatcttga tcaaaacaat agagatgccc ttgttcacta agtttggaca
     7681 acaaataagc tgagtggcct gaaccaccta gcgttgcatc aacatatatc ccattaggct
     7741 tgatgtcaag catatccact gtttcgtgaa gtagtacggt cacgtgatga aattcttttg
     7801 tcataacttt aattgtacca tacttcaaaa ggtttacaaa tgaaaaagta agagaaaagg
     7861 gtttcattcc cgagagaatc ttccttttct cttactttat cctttttgtt agttatcagc
     7921 taagtcaact tagcaagtgc taatttagcc gctgcctcta gagtttcaac tgcttgtaaa
     7981 agatttttag cttctttaat cacacaacaa tctgtcagcg ggttgtctaa ccaaatacca
     8041 aaggttttat cccaactcgt atttggtaag tcagctaata atagcctatc gacgtctacc
     8101 agctatcttc ggcagttttg aagagtaact tgtaagtcta ttaataattg gttgacctta
     8161 ctcattattt cgacgtttga tccagctgac aagcatacca tctccaaaaa aaaaaaaaac
     8221 aaaatagccc caatttcgga caattttcaa ttccaaatga atcacctgaa tctgatgtaa
     8281 aagttcttct aaagcaacct ttgcttcttc cttttctctt tcattagtca ttaagggacc
     8341 ttctagtagc ttattctctg atatgtccat ccccattaat ataaaattta gtgctggtca
     8401 aggcttctaa gcccattggc ccacgggcgt gcattttttg agtagaaatg ccaatttcag
     8461 caccaagccc aaaaacaaaa ccatcggtaa atcgtgttga agcgttgaca taaacagcag
     8521 cagcatccac taaatcttga aaggtctctg ctgccttaat atcccttgtg atgatagctt
     8581 cggaatggtg actagtatgc tgatttatcc aagaaatagc ttcttccaaa gaggaaacaa
     8641 ccttaacaga catgatataa tctaaaaatt cggtctcaaa atcctctgct tttgcaggca
     8701 ctgcctgctc aaataacggc aaagccttgt catctgcccg ccattctact ggctgaacct
     8761 gattgatcgc tttttcaagc ataggaataa accttgctgc tacagcttcg tgaattacca
     8821 atccctcagc tgcattgcaa acacttgggc gctttgtttt agcgttgata acaatcttag
     8881 tagccatatc taagtcagct tgagcatcca catagatatg cacattcccc acacctgttt
     8941 cgatgacagg cacttttgct ttttccttaa cggtctgaat taatctagca ccaccacgtg
     9001 gaatcaaaac atctacataa tccgttgcct gcatcaattc ttcagcaaca gcatgtgaag
     9061 gatcctcaac tagctggaca gcatctggag tgatacctga ttcttccaga ctttgtcgga
     9121 tcaacttcac caaagcctta tttgagtaaa gggcatcctt tccccccctt aggatgatgg
     9181 cattattggt tttgaacgcc agactaaaag catccactga aacattcggg cgactctcaa
     9241 aaatcatcgc aatcacccct agaggaactc tcttttggag gattttaagg ccatcgaggt
     9301 tagtgtagcc cttaatgact tgtccgattg gatcagctaa gtcagcaacc tgttgaactc
     9361 cctgtgctat cgctttaatc cgttctgaag ttaagcgcaa gcgatccatc atgatgtcag
     9421 aaataccatg ttctttagca ttcgctaaat ctttttggtt agcggctaag agagtctgtg
     9481 tatcgtcaac taataccttt gctaaggtgc ttaaaaagcg atttttaatt tgcgtcgaca
     9541 aaggtgctat taaaagacta gcttgttttg ccctttgccc taaccttctc atatcagtca
     9601 tgccttacct cttctatctg cttgaaccag gtgcctagcg gttgcccttc aagcactctt
     9661 aaaatatctc tgggattagc tccattcatc aagaccatct gtcctttgtt ttcaaatacc
     9721 atctgggcag attgaatttt gctaagcatc ccacctgtgc caaacttact gcccgcacca
     9781 cctgctgacg caatgatttc ctgcgtaatg actgcaacat gactgcgtaa ctgcgcatcc
     9841 tcataaatag tagggttttt atcaaaaagg ccatcaatat cagacagcat gattaataaa
     9901 tcagcttttg taataccagc aacgacagca gataaacggt cattatctcc aaatttagtc
     9961 gcatgatcca tttcatccac actaaccgca tcgttttcat taacaatcgg cacaatgccc
    10021 aaactaatca gactttcaaa agcattggta acattggcta gactttctgg gaaaaccacc
    10081 acatctctcg tcaataaaat ttgtgacacg ttggtttggt agtgtgcaaa aatttgtgaa
    10141 taaagactca tcatggccac ctgaccaaca ctggaaactg cctgttgttt agcaagattg
    10201 gtcggacgtt tttccatttt caaaatatct agtccaaatc ccatcgctcc cgatgatact
    10261 aaaataactt ccttgccctt attcatcaaa cttgaaatca caaaagccag ttggtcgatt
    10321 ttttctaaat tgattttacc tgttggtagc actaatgaac tagtgccaat tttaattacg
    10381 atacgtgtta catcttcaaa ttgtcgtttc atcataccca aattatagca taaaaacagc
    10441 cccagaggac tgtttttccg aataatgacc tctcttcgtt aaagcttctc taagctttgc
    10501 cagaaggttt tttgaatcca taagtgagca atccctaaaa ccattagtgt cagacaactc
    10561 agtagaacac taatcaacag aggttgttgg ataatattgg caatgataac agctccaaaa
    10621 cctactacac ctgtgactat taaattacca aagataaggc ccattgttag ccactgccca
    10681 tttcctctgg taaagagttg tgtcatgtct tgccatttta aatccagaag ccggtagtca
    10741 cgacgataca taaactcccc ttgcacaatc agagacaggg cgtagccaag acaaaaagca
    10801 atcgttagaa gaggatgcag gtgcaacaca aagagaccaa acacaagata aatcaccata
    10861 ggcacaatca gttgcagacc gacgagcaag caaaatttat ccagcaagaa tttttttaac
    10921 gttattggta agcttttaat aaaggtaaag ttatcctttt caagtgaaat acctactcca
    10981 ataaagctgg ttggtgttgc acacatgata cccaaactga ccccaaataa taaggccacc
    11041 ccaaagtaat ctggggaaat atgcttaaag aaacctgtgc cacgtgacaa acttggaccg
    11101 ataaaaagca tcacatacat caggggcatg agataggttt gtgtcagtaa agttgcattt
    11161 tgtaacgtta aaaggtgatg ttttcgcaac aactgcgcca gtgattgatt ctgatgagga
    11221 cgattaactg gttttttagt ttgcttggct ttgttctcgt tactaatata aaaagcctca
    11281 cgataataat ttggcatcac ttttgtgaca ataccatata ccatagctag aaccagtagc
    11341 aatggcaacc aaaagttaag gagggccgca gtagaaaacg gtgcctgaat aacatcatag
    11401 aatcctttga aatacggaat agttgggtaa tcagtaaata cgccatccgt catcgtacgc
    11461 ttgttgttac taatattaat cgcaaaaatt aagacgaaag cgccaaaagt tgagacaaac
    11521 atcatgatag ttgaaatgag tttccgcttg cgactcctta caatgatttt gcccacccaa
    11581 gcattgatat aaatagctaa taccatcgaa ctcactagta acactaaaaa gagcacgata
    11641 gctactaaaa tagacaaagg atttcccaat agttgccagt aggcaattaa gagaagagag
    11701 ataagaggca tcaaaaagac agcacccatc cctagcgacg agacaatttt tgcaatatag
    11761 agttcttctg acgttactgg taagtgaata taaagtttta ggtcattact ttcataaaaa
    11821 atggtgtaaa gagagctaaa ggctgtcaag gttgacatga taaaaaacat agcaacgtca
    11881 aaggagaaaa gtcctggata atgactgaaa tcaacaccaa taaacataaa aaggtaaatc
    11941 accaaaaaca tggcaatcat taaggcctgt tgcctcatca tgcttttata ggctttgaag
    12001 ttttctttcg ggtgtttttc ctgacgcttt ttgaggttag ctaaactttg tggattggaa
    12061 taaagaatgt ttatcttgat aagttcccaa atagtagacc aattcatccc atcacccctc
    12121 ttcttgcgcc ttacgccctg cgagttcaag gtagatgctc tccaaatctt tgtcaggatg
    12181 gtgttctttt aactcatcaa tcgttccaac aaaaatcaat ttcccttttt ttagaatccc
    12241 gatacgatca cataactgct cagcgactga tagcacgtga gtcgaaaata agaccgtatg
    12301 tccagaagct gcatgagctt tcatcattcc cttaaggtca aaagaagcct gaggatccaa
    12361 acctgttaat ggttcatcca aaatccagat gttaggattt gatacaagag caccaatcac
    12421 aatcactttt tggcgcatcc catgtgagaa actatcaatg gtttgattaa cttcttcctt
    12481 tagttcaaaa agtgttgtca gctgtgctaa ccgttcttct cggtcctcat ctgacacccc
    12541 ataaatttta gctaaaaact gccaatactc atttggagtt agattcaaga aaatatcagg
    12601 agaatctggc acataagcaa tttgtttttt gatagcttct ctattttctg tcagaagttg
    12661 accatccaca aatacttcgc cataactcgc ttcgataata gaagtcaaaa tgctgatggt
    12721 cgtggtttta cctgcaccat tatgtccgat gaggccaaaa atttcaccgt cattgatggt
    12781 gacgtttaaa tcgctaagag cttctttgtc gccatacaac tttgaaacgt gtttaaattc
    12841 tatcatggta tcctttttcc ttttcttctc tttttctccc tagactgatg aggcttcctc
    12901 taaaatatac ttttccaatg actgcttaaa acggcaaagc gattttggaa gtaaaatgtc
    12961 attatcaagt aagtaattat ccacatcttg gtttagttgg taggcaacaa gaagtgggtt
    13021 actgtgttta attaatctat tagaagctgc taaaagtagc tgcgacaagg ccacattttc
    13081 acgattttct ggataatcat aagcgtgtct aatcacatcg tataagcgct gtcgtttctt
    13141 ttccatttat actcctttat ttttgtactt ttacgctaat acaataacaa ttaaattata
    13201 atttttatga attgttttgt caatgacttc ttatcaaaag aagtgtatta ggcagtttga
    13261 ggaaaagaaa gaactgggta ttttatttta aaaaaacaga gtaaatcaaa tgatttactc
    13321 tgtttgggat gtgtaaccta gagtgatttg tataactcag ttgcatttgc cacagtgaaa
    13381 ccatattctt cgaagatacg atttcctgga gctgaagcac cccaagtgtc aatggtcaat
    13441 gtttttcctg ctaggccgac atattttgcc cagccaaagc ttgaacctgc ttcgatagcg
    13501 agacgtttag tcacagctgc tggtaggatg ctttctttat actctgctga ctgttcgtca
    13561 aagatatttt gtgatggcat agataccaca cgcacatgaa tgccttctgc ttcaagagct
    13621 tcttgagtat ccattgccag tttcacttct gaacctgttg caatgatgat accatcaagg
    13681 tcgcctttgg cttctgataa aatgtaagct cctttgttaa gaccatcttc tgccaattct
    13741 tttgttcctt caagcactgg aaggttttga cgcgtcaata caagcatagt tgggcgatct
    13801 gtttcagcaa tggcacgttt ccaagcagta tttgtttcgt taccgtctgc tggacgaata
    13861 acgttcaagt taggcattga gcgaacactt gccaattgtt caattggttc gtgcgttggc
    13921 ccatcttcac caacagcgat agaatcatgt gtcatgacat aaactgttgg caaattttgg
    13981 agcgctgcca tacgaacagc tggaagaaga tagtttgaga agacaaagaa ggttccgccg
    14041 taaacgcgtg tcccgccgtg aagggcaata ccgttcatgg ctgcagccat ggcaaattca
    14101 cgaacgccaa accagatgtt acggccttcg tagtgacctg attggaaatc tgtttcagcc
    14161 ttaaccatag tattgtttga agctgaaagg tctgctgacc caccccagaa agaggctact
    14221 tgttctgaaa tttgttggat ggcttgttga ctggatacac gactcgccat tgaactacca
    14281 agttcgtgcg cttcaagttc tacttgagca gcttcgttag taaaggcttt ttggtattct
    14341 gctgctaatt ctggatattc tgcttcataa gctgcaaaca agtcattcca ggcttgttct
    14401 gctttttcac cacgcgcttg aagaccttga gcaaagcgct ctgttacttc agcaggcact
    14461 tcaaaatctt gatgagtcca ttggtaagct tttttggcaa aagcaatgcc ttctgctcca
    14521 agaggagcgc cgtgaacagc tgaagttcct tgtttttcgg caccaaaacc aataatggtt
    14581 ttaacttcga tgatggttgg tttctcagtt tcagctttag ctgcttcaat agcagctgca
    14641 atttcttcta aatcgtttcc gtctttcaca aggatatgtt gccaaccata agcttcaaaa
    14701 cgtcctttca catcttctgt gaaagccata gaggttggac catcaagaga gatgtcgttt
    14761 gaatcatata gcaagaccaa tttcccaagt tttaaatgtc ctgccatact tgctgcttct
    14821 tggctgaccc cttccataag gtcaccgtca ccattcaaag caaaggtata gtgatcaacg
    14881 atgtcaaagc ctggtttgtt aaatttagct gctaggtgag cttctgccat ggccatacca
    14941 acagcattcg caatcccttg accaagaggt cctgtagttg cttccacacc atcggtatga
    15001 ttaacttctg ggtgacctgg tgttttagaa ccccattgac ggaagttctt taaatcttct
    15061 acagttaaat cataaccagc taagtgtaac aagctataaa gcatagcact tccgtgtccc
    15121 gctgatagga taaaacggtc tctgtttgac caattacggc ttgtttttgg attaatgttc
    15181 atgaagtgat tccaaagaac gtaggccata ggtgcagcac ccattggaag gcctgggtgt
    15241 ccagaatttg ccgcttgaat ggcatccatt gatagggtgc ggacagtatt tactgccaac
    15301 tggtcaattg catcaaatgt cataagtaaa acctctctgt ttattacaac taaaattaaa
    15361 cgttttctat tgtctattgt atcacaaaag tcaattcttt ttcagaaaat cctctaaagt
    15421 ttctaaattc ttttctgcta gaaaaataac taaaaacgtt atggaataag ctcatatcac
    15481 tttctttttg actatcaaat aagaaagtct gttgacacgt tggccacaga ctctttagcc
    15541 ttatttttta aaggaagccc aatcttccat aaaggttttc aatccatttg ttgtcaatgg
    15601 atgttgagtc atcttagcaa ataatgggtc tggaatcgtc gcaatatgtg ctcctaattt
    15661 agcaacagct tccacatggg ctgttgtacg aatacttgct gcgatgattt ctgcttggaa
    15721 atcgtacaag tcaatgattt cacgaagatc gctgattaat tgataggcat ctgtgccaat
    15781 atcttccaaa cgaccgataa atgggctaat gtaagttgcg cctgctttca ttgccattaa
    15841 accttggctt actgtgaaaa tcaaggtcac attagtttta atcccttctt ttgaaaggat
    15901 gtttgttgct tttaaccctt ctgtggtcat tggaatttta acgacgacat tgtcatgcca
    15961 tttagcaatg cttctagctt cttctaccat agcatctgct gttaaccctg tcacttcagc
    16021 actgattggg ccatcgacga tgtcacagat ttctttgata actgtttcaa aatcacgtcc
    16081 ttcacgtgag ataatggttg gattggttgt gacaccatcc acaacgccaa gctcattaat
    16141 cgctttaata gcggctacgt tagctgtatc taggaaaaat ttcattgttt acctccaaag
    16201 tttttcaata tattaacgct ctaattgaca acagtttatc acctatcgcc tagagtacct
    16261 acctaattat tataaaagtc tgacagccct tagaaaagac caagtatcac acagagttgt
    16321 gttcaaaact agacttagct tttgggctaa ccagcggttc ttcctcttgt ttattttttt
    16381 gttgaaagag tgatttgaga aaatcctcta aggcagcaag ttcatagggg gagctatact
    16441 gttttaaccg gtaatagggt aaatcttgtc tcggtaaata gacagtctga taggtaatca
    16501 gacagtcata aactttttga tgatcatagg gcattaattg aaaatcccta cgatgcccca
    16561 aatgacgtct aatggccaat tccgcaatgg ctgcctccac cttattttgc cccaaggcaa
    16621 gccctactaa aagaggctca tggtggcgct caatggaaaa aaccaacaat gctaaaatgt
    16681 cgtcactcaa ctgctgaccg aaatccgttt cctgacccat ttgcagcact aaatggttta
    16741 tgataaccct taaaagcgct cttttatctt cgcctactaa ttgataaagg tattggctat
    16801 tggttggttg gctaaggata gctcctttaa aaaagtaagc tttggaaaaa aatatcccca
    16861 agccataaat gatttcctcc tttgtctgat ggataatcac atgagccttc tttaaaagcc
    16921 ataaggcttc tgaaatgtga tcagcaatag gacctccaaa acccaaacta tacttcatgc
    16981 tggcaatagg aagtagagta taagcatgca aaaagacaaa gaggctctta gcttcaaaag
    17041 catcaaattc aagggcatag cgtcttaagt aatgtaaaag catgctttct aagcgcttaa
    17101 agaaaatatt agatgtcata aagtcactat ctcttaaaaa atgatcattg tagccttttt
    17161 gaaaggacat tcgcgcttgc gaaatcgcca accaaatcaa taattgttct aaggcttctg
    17221 ccgataatga ctggccaatc agccgctcaa ttaaggtagc caaatgcgaa gcgtctaact
    17281 gattgaccaa ggcgtctatg ccttgtctgg tcaaggtatg gcggaacaat tcaaaataga
    17341 aataacgcca ctgaagctca cttcccagtt gtctgccttg cgagagcgcc agatcaaatt
    17401 ctgataaaag ctggttgagg tgagctaatt gtctgtttag agtagcttca ctaaccatca
    17461 gttcaacgga taagttggtg atgttaaagt gttcatggca gtacatgtag gtcaatatct
    17521 gataggcaac cgattctttg agaagcagag ctaccaaatc ctcccaatgg aactggccat
    17581 tttcttttaa gctcagactg tctttttcta aaagtaattg acaggctaaa ccttgttctc
    17641 gacacaaatg atttagattg tcaatgtatt ttaatagggt cactttagac aagtttgata
    17701 gcctcattag gtcttttagc cttaacgttt ctttagcatg ataaagggtc accaagagac
    17761 gatattgggc ccttcgttcc ttatccatta agtcttcaat cttcatggcg gagcctcttt
    17821 tcaaataatt tgagacaagc ttcatgaaga tgataagcca aatcactgtg accttgtaaa
    17881 aagagaaaat cttgaaatgc taagtcagcg tctgtaactt tcagagccag agcctgacct
    17941 aattgattgg cgatgacaag agcattggtt ttggatagga gctgagcgcc tattaaaatc
    18001 ccatccgttt ttctagctat taatttcagc caaaagaggt ttttagaatc cccattttgg
    18061 tacaaatagc tacacgtatc catataaagc tctgcttctt cttctgtcac accaacactc
    18121 gcgcgataat aaccaaagtg agaacttcct aataccttga ccgtctgtaa tggaggaata
    18181 ggatatccag ctaaatgcag agccagagcc tgacctgtct tcattgcttg gttaatcaaa
    18241 ggtgtatagt aatccattcc caatatacca aaatgcaatg aaaccatgtc tccaatggca
    18301 tagatattag cctggctggt ctgtaagttt tcattaacca aaatggtctt atccaaagca
    18361 caagccactt gtccttgcaa aaggtgtgta tttggcctaa agttaatggc taaaatagcc
    18421 aaatcacctt ggaaggtgtc cccttgctcg gtatgagcga caacttttcc ctctatctca
    18481 tgaatagccg taacatgctt attattaata aaggtgacat ggtgaatgga catttctgcc
    18541 attacagggg ctatcagttc tgcgtcagtc tgatagaaat ctggacgttc ggctgcttca
    18601 atcagtttga cccgtttgcc ttgtaaggac aaactgtagg caaggtctaa gccaatcaag
    18661 ccggcaccaa taaccaagac ctcttggcta gcctcaatca gttttaaact agcttggctt
    18721 tcttcataat atttggttac caaaactttg ttggtctgtg acgtctcaat ataatgagac
    18781 tgggggctag cacccatggc acaaacaagc gtttcataac cttcttcaaa aactcgtcct
    18841 tgactgtctt tgagaaacaa ggtgtttgag ggagcttcga tagctaacac ttctgcttga
    18901 ataaaccggt ggttagattc tatttgtgct gctaggcagg cccttcccca catagcatca
    18961 gacaggtctt gaatgtctcc tctgaataat tggttgatac catttggaat ataattggga
    19021 cagctttcct tatcaatgag aatgatttgg gaatctggat tgaggtcctt atatttatca
    19081 acaaaggcta gaccagcaaa ggatgccccg ataacatgta tggtctttgt cgttaatgtc
    19141 ataatagtat tattatagca gttgttttta agatatgaaa gcgttttacc taatcctata
    19201 aagaaaaagc aaaggagctg gcctatgata gccaacccct ttagtgatgg ttaggacatg
    19261 acttggaaaa gcaaaactgc taaggccgct ccaataattg gtcctacaac aggtatccaa
    19321 gcataagacc aatcagaatc tcccttgttg ttgagtggta gaatagcatg aactagacga
    19381 ggtcctaaat cacgcgctgg gttaatggcg taacctgtcg ttcctcctaa ggataaccca
    19441 atcccaataa ccaaggtacc tacacagagg gtccctagtc ctgctggcat atcatataag
    19501 ccaaatgcca aaataccaag catcaagaca aaggtcccaa agatttcact caataggttt
    19561 gagctggtat ctcgaatagc aggacctgtc gcaaatgtcc ctaaaatatc agcctgactc
    19621 tcagcagcca gataatgtgg cctgaactga aggaatacca gagttgaacc aagcatagct
    19681 cccaatagtt gggcaaggct ataggcaatg gctgttgacc aggcaatggt tccactcatg
    19741 gcaaaagcaa ggctaacagc tggattgagg tgggcagggg caactttgcc actgataaag
    19801 actgccacag caactgcaat tccccagcca gtcgcaatca cgatccagcc agaagcatgg
    19861 gttttcgttt tgggcaagac aacccctgat accacaccat ttcctagtaa aaccaataag
    19921 gctgtaccca aaaattcgcc aaagatatcc atattaaccc tcctgatggc cttttaaggc
    19981 actcaaatca ttgtctgcaa tcacgcgacg aagatcgtct tcttgtgcca ctctttcctg
    20041 atcagaccat tcaaaatgtt tagccatttc attaatcact gggtcaatca aagcatctag
    20101 gctatctcgc ataaataaga ggtgatttgt tcttctcaag aaataatctg tcggtttaag
    20161 agccatttca taatccatcg cataatgtaa ggacaaggtt tcagctaaac ttaacccttc
    20221 agctgctgtt aattgacgag ttaaggcaaa gacttttggc gcattagaac cataaaggtt
    20281 tgccaaatac ctagcatcat ccatcgaaag accactaaga gttcctaatt gagcataggc
    20341 ttctatttcc gaatctacat tggctggatt gatttcacct cctgaaacag gataggtttt
    20401 tgaattgata agcttgaagg atttgccaaa ctcttctttg aggatttgaa taatccctgt
    20461 caatgctcct tcagccattt tgcgatagtc ggtaatctta ccacctgcca aggtaaagag
    20521 tccattctca tcacggtcaa aacttgaacc tcgtgacact gcagacggat ccaattcttt
    20581 ttcagatgtg ctggtttcaa cctgcttaat agctttttca acagcttctc ggctatcttc
    20641 gtggttaata taggctttga cagtatcaac caagtgatca aaactatcat cactgacttt
    20701 accgctgttg ccaccattgt agtcagaagc actattacct gataacaagg ggcgaagacc
    20761 agcccaactg ctttcaatat catcaatggt cacgttggca tttggaaagc ggttattgac
    20821 aacgcctaac aaataatcca catcttcttg agtaacttgt gggtgctcca agtctccagt
    20881 gtagtccgtg tccgttgttc caaaataagt tttttcttca cgtggcaaga caaagaccat
    20941 acgaccatca tttaaccctg tgtcaacata gacaggttga gacactggta atttttgacg
    21001 gtccactacc agatgcaccc cttttgtagg gcgcatttga tgaatcggtc gtcccttatg
    21061 agagaattga cgaatctcat cactccatgg acctgtggtg ttgatgacta atttagcctt
    21121 gataatgatt tcttgatctg acagcaaatc acgcgccttc acaccaataa tcttaccatt
    21181 gtcatctagc aagaaatctt ctgctttcac atgactagca ataagagccc catcacgatt
    21241 agctcgtttg atattttcaa taacaagcct tgcgtcatta ttacggaaat caaggtaaac
    21301 cccaccacca agcaagcctt cttgttttaa gtctggttct cgttttaaga cttcttcttt
    21361 ggttaacacc ttgttggccg ctggcgtatt ggacacgcct gctaaaagat catacaaatc
    21421 catagcgacc ttcaaacgga acatgctaaa ggtactacca ggttcgtcat aaacaggtaa
    21481 taacataggg tctggttttg gaatgtgggg tgcaatttgt tgcaccacag cccgctctga
    21541 caccgtatct gaaaccacct ccacatcaaa ttgtttcaag taacgaagcc ccccgtgaac
    21601 caatttggtt gaacggctag aggttccttg cgcaaaatct tgcatctcaa tcagacccgt
    21661 atctaggcca ctagctgccg cctgaagcgc cacaccagca cccgtaatac ctcccccaat
    21721 aatcagtagg tctaaatctc tttcttgcat tttttggaga gctaagcgtc ttgtttctct
    21781 tgaaaattcc attccatcct cctctagtta ttttgcatcg tcatctgcat cttcttcttg
    21841 agtgaaaact tgagttgctt taacagcgcg cttccaaccc ttgtataatt tttctttgcg
    21901 cgattcattc atgctagcct taaagagctg gcctgtggca tttaactctt tcaaagcatc
    21961 catgtcttcc cagtagccca cagcaagccc tgctaagaaa gctgctccta gagctgttgt
    22021 ttctaaattt ttagcacggg caatatcaat tcctaaaata tcagcctgaa attgcatgag
    22081 catattattc atggctgcac cgccatccac acggagctgt tgaatgtcaa tacctgaatc
    22141 cacctgcatg gtatcaataa catctcgcac ttgataggcg atagattgta aggttgcttt
    22201 gacaaaatct tctttgcttg taccacgtgt caagccaaag acagatccac gcgcattaga
    22261 atcccagtaa ggagctccta aaccggtaaa agcaggcaca acataaactt catcgtcact
    22321 agttgatgct agagcgaact gttcagattc tggtgaagtc tcaatcattt taagaccatc
    22381 tctcaaccat tgaatggcag aacctgcaat aaagatggaa ccttccaagg cataatgtac
    22441 cttaccacca ataccatacc caatggtggt caaaagatta ttactcgata actgcatctc
    22501 atcaccagta ttcatgataa tgaacgaacc tgtgccataa gtgtttttca ccataccagg
    22561 ctcaaaagct agttggccaa acaaagctgc ctgttggtca cccgccatac ctgaaatagg
    22621 cacttcgccc ccataaaaat gaaaggcagc tgttttgcca taaatttcag aattggattt
    22681 aacctctgga agcatatctt ttggaatatt taaaagctct aaaatctcat catcccatgt
    22741 taagtcttta atattataga gcatggtacg agccgcattg gaataatccg tcacatgaac
    22801 agcaccatct gtcaatttcc aaaccagcca agtatcaatg gttccaaaaa gtaactcgcc
    22861 tttttcagct cgttcttgag cacctggcac atggtccaaa atccaacgga tcttggttgc
    22921 agaaaagtaa gcatcaataa caaggcctgt cttttcatgg atcatttttg tatggccatc
    22981 ttctttgagt tgctcagcaa ttggtgccgt ctgccgtgat tgccacacaa tagcattata
    23041 aataggtaca cccgtttttt tgtcccaaac cactgtggtc tcacgttggt tggtaattcc
    23101 gattgcttca atttggcttg gtttgatgct ggactcaata aaagcacccg caataacaga
    23161 ctgaaccgaa ttccaaatct gattagcatt gtgctctacc caaccagcgt gtgggaaaat
    23221 ctgagggaat tctttttggc tactgctaac cttttcccct ttttgattga aaataatcgc
    23281 tcgtgaactt gttgttccct gatcaatagc catgatatac ttttcttgcg acattcatta
    23341 ccctccttta agtaagcgtt ttcctatttg attctattat agctaatttt atcagaaata
    23401 tattatcaag aatttttaaa gctaaaaaaa agctgataag ataggtagct tatcccacta
    23461 gatgatcttg aaaaatccac tgatgacaac cacgtctcct catttctaac gaggtaatct
    23521 ttcttatcaa agaaaatcaa aagacccaac aatcactctg ctaatccaga gtcatcattg
    23581 ggtcataaag agaactaagc ctcaaaggca acacggtatc agacagccgt gcctaagagg
    23641 caaagacaat tggacacaag agttaaggct gtcttattca ttacagttct tgattagtca
    23701 ttgattggtt cagaagattt ttccacaata tcccctgagt tggcgtcaat ggtatagctg
    23761 tattcttgat ctttgttttg aaactcgatt tcatacacgg ctttgccatc ttcattatct
    23821 tgtgtaactg atagcatttg agcatcagct tccgtaacag aagcatcttt taacgcaatt
    23881 gatttggctt tgtcttctga aagtttaact ttcttactag tttgacttgt ctttggttga
    23941 ttatccatat gatcgtcttt atcatccata tcacaggctg caagactgag taaagagagg
    24001 ctggctatgg ctagtagaga gaatttttta attgtcataa gtactacctt ctttctttga
    24061 tttcttctat tataactcaa acacatggtt tttcccaata ttttgactat acaatataaa
    24121 aacgtgtggt cactttgaag tatcatacca tttaaaataa aagctaacag tcatcacgat
    24181 aaacaaaaag cagttcagag ctgctttttg tcttatgatt ttgggtcatc taatgaacgg
    24241 ccgtgcaagc ctttttcacg ttgcacctgt cttaattttt caggggtcac gtcgtttcct
    24301 tcttcgtcca ccaatttgat gccttcgata tggtgtctga cagagcgacg atagccttca
    24361 atgtattctt cacggagttt tgcctgttcc actttttcag gtccagtcaa gcctaccgtt
    24421 tttttctttt tagccagctc attgatacga gcaatttttt taggatccat gttaagccac
    24481 taagagcctg gaaaaaaagt gtaaggctta aaaaactgac ttgactctca ctgtgagaag
    24541 caatgtttaa aggccttgtt ttttccactc gggtactgaa ctcacttttc tagctaagtc
    24601 cccaacggct ctttcctttc tatttacttg gtgtttagtt ggttaaaagc agcaatggcc
    24661 ttagctttgc ttactaaatc tgataaaata gctaaacggt tgttcttaag ggcttgatct
    24721 tctgccataa ccattgtgtt atcaaagaaa tcattaatca ctgggctaag ggcaaagacc
    24781 tgttctaact gctgggcagc actgcctgct agagtcaagc cttgaatagc tgcaaacaaa
    24841 gtgttttctt gctcattttc aaagaggcta gggtccacct gaacagaagc gtccgctttt
    24901 tctgccaaat taaaagcacg tgacaaagat tcaactgctg gtttgtaatt ttccgtatgg
    24961 ctggctttga caagtgcttc agctgccgct aacatttcag gaaccacaaa ggttgaactc
    25021 tctaaaactg cttcacgaat atcttttgga actgctttgc ccatcatctt atccacacga
    25081 gcacggataa agttcatcac atccgcctta ttggcatagg tcaaactatc aaaggacaag
    25141 tcatataagc tatctaccag cttatccatt gggatgcgcc atccaaaatg atctagaata
    25201 cggacaatcc cttgggttgc acgacgtaga gcataagggt cgtttgaacc agatggaatc
    25261 aagccaacag aaaagaatga caagagagta tccaatttat ctgctaaagc taggacagcg
    25321 ccaactttgg tttcaggtag cgcacctcct gcagcatcag gtaagtagtg ttcacggatt
    25381 gctgttgcca cagcagcatc ttcaccagct agacgggcat atttttcacc cataatccct
    25441 tgcagctcat caaattcacc aaccatacct gttaaaaggt caaatttgta aatttgggca
    25501 gcacggtcaa cagctgtgac ttcctcagca gataggttgg cttccttagc aagtgatgcg
    25561 gcaatgacac gcgtacgatc catgtgttca gcaagagagc cgattttttc atggaaggtc
    25621 acattggtca atttggcaac caaatcagca atttgcaatt tctgatcctc acgccagaag
    25681 aattcaccat cttctaagcg agctaccaaa actttttcgt tccctttaat aacattttca
    25741 atggcttgat cattcccatt acgaactgat acaaagtttg gcatcaaatg accttcttga
    25801 tcacgtacca caaaatagcg ttggtggttt ttcatcgatg tcacaagcac ttcctcggga
    25861 acatcaaggt atttcgcatc aaaacttccc ataaaggccg ttgggaattc gaccaagttc
    25921 aagacctcgt tgagcaagtc agcatcaata tccacttgta ctccttgggc cgcctcaatt
    25981 gccttgattt gctccacaat catctcttga cgctctttag catcagcgat aacaaactgg
    26041 ctgcgcaaat cagcctcata agaatcagca cttgtgatag tggtttcagt acctaaaaaa
    26101 cggtgaccac ggctgacgcg tccagaatga atatccaaga agtcaagttc caaggcctca
    26161 tcattcaaca gaactgtcaa ggtgtgaaca ggacgaatgt attcaaaact attgttagcc
    26221 cagtgcatgc tgactgggaa ggtcattgct gacaacactt cagtaacgcc tagaagcact
    26281 tccttagcag gttttccagc ttcgtgtttg gtcacataaa cgtattcttc tcctttgact
    26341 tcgcggaatt cgatagcatc tgtggtcaag cctttgccac gaacaaatcc ttgagcagct
    26401 tttgagaaat tcccatcagc atcaagggca attttcttag caggcccttt aaagtcctca
    26461 gtcaaatccg tttgctgatc agctaaccca gacacacgca ctgccaaacg gcgtggagtt
    26521 gaaaaggttt gtatatcttc aaaagagaga cgattttctg ttaagaaagt cgccaggcgc
    26581 tctcccagtt gtttttcact tggtgtgacc acgtaggctg gaagttcttc cagaccaagt
    26641 tcgattagta gatttttact cataattaag ataccaaggg cgttaagtgg acaccatcaa
    26701 aatagcaaag cgacgcaggt gcttgcacct aggaagtctt ggtctttttg acatagtccg
    26761 tagcccgtgt tcagttcctt tctctaaaaa gtatcttttt gattgttccc ttaggtaggg
    26821 gggacgcaaa gtgaaattat ctagtggata atttcagaaa acaaaagtgt tccattccta
    26881 gttagccagc ctaaagtctc gccaactccc tcgttcgata agtaacctta ccgaattgtc
    26941 actacagcgg gagtaacctt gactcggcga ccttcggtct cccacttctt gctcgctctt
    27001 aagattacaa agctcgcaac tagatcatct ttaaatcgac ttattcgtca tcttcagcta
    27061 gcaagattgc gcgtgttgct tcatccaaca gtggaaatcc aagcttcttg cgttctgcaa
    27121 caaaggtctt agcaacgaca cgtgccaaat tacggatacg agcaatgtag cctgcgcgtt
    27181 ctgtgacgga aacagcgcca cgggcatcca gcaggttaaa cgtatgagaa catttgagca
    27241 cgtaatcata agctgggtga accaaacctt cttctagggc acgcgaggct tctttttcaa
    27301 acttttcaaa gttttcaagc agcatgtctt ggtctgacat ttcaaaggaa tattttgaat
    27361 gctcatactc aggttgcaag aagatttcac catatttaac acctggtgcc cactcaatat
    27421 catagaccga atccacttct tgaatatagg aagctaagcg ctcaagacca taagttactt
    27481 ctgctgtcac tggagatgtc gcaaggccac caacttgttg gaaatacgta aattgtgtga
    27541 tttccattcc atcaagccaa acttcccaac caagccctgc tgatcctgtt gaggggtttt
    27601 cccagttatc ttcaacaaaa cgaatatcat gttctaaagg attgatgccc aatttttcca
    27661 aagaagcaag gtaaagttct tgaatatttg atggtgatgg tttcatgacc acttgaaatt
    27721 ggtggtgttg gtaaagacga tttgggtttt ctccgtaacg gccatctgca ggacgacgtg
    27781 atggctctac ataagccgca ttccatggct caggacctat agcacgtaag aacgtgtaag
    27841 gactcatggt cccagctcct ttttcattgt cataggcctg catgagcata caaccctgat
    27901 cattccaata ttgttgtaag gtcaagatga tttcttgaaa ggtaagtttt ttagacattt
    27961 cgtactcctt tgatagtagt aaaatggtat atcattgaca atcgtttttt tgtcatcagt
    28021 aaaatacctg acgatgttac tagcatgtat tacaggtcta ggcaccaaaa aaccgcattt
    28081 tgatgctcct aaatcataag acctaggggc gtcaaaacgc ggttccaccc taatttatta
    28141 cttcgtttat atagtatctt tttgggcaat attgacaaat atacttaatc acactgccca
    28201 aacggaagcg ccaatctgca gtcctaccat tcggctctaa ctaccccgaa ctcgctttag
    28261 ataagatact tgcatccttt ttcctagagt ctagtttagc ataaagccat tagtttgtcc
    28321 atcatgagct taaggttttg ttcgagtacc atttggaata atcacttccg ctaatagctt
    28381 gttttcttct cgaatacgta aatatattac tacaatgtag catgggaaaa gaagcatagc
    28441 cgtaatttta gcatggcaaa gtaaggctat gcccaccagc tcaggggcga tatttaaaaa
    28501 gtagttagga tgtttaatcg ttttgaacaa ccagtgatcc acatatttgt ggttttttgc
    28561 taacatcaat tttacagtcc aaatgtcacc taagatacgt gtcacttcat ataaaacagc
    28621 tactgataat aacatcaaca aaagacctaa aaaactaatg ccatcaaact taatttgttt
    28681 aagcagcgct tcaatcaccg caaacacata aatcataatg tgaagtaacg tcaaaaattt
    28741 cgtgttctga gcaccaaatt cctgtcctcc ttgggctaat atggcttttt catttgttat
    28801 cgatttcttc aaaaatacta aacgaatagt aaacatagct aacatcacta aaaagatggt
    28861 cacaatacgg ctccttattt tcaataatag tttctttcac aaaacactaa taaagattat
    28921 agctaaatga taaacctttg tcaaaccgtt ttcaaaaaag atttagcgaa ttatggctaa
    28981 atctttgtag tttcttaaaa acttgtagta tcagggttag gaatcgctga cattcctgag
    29041 agatagcgaa ttgttttttt atcttcttgt gtcaagctca catcaaaaat agccatattt
    29101 tccttgatgc gctcatcatg caccgatttt ggcaagggaa taaaaccttc tgccagcgac
    29161 caagctaagg caacttgtgc cactgttttg tcatatttat tagctaactg ctgcatagtc
    29221 tcattatcaa aaatctcccc ttgtcctaga ggactccaag cttccaataa aatctcattt
    29281 gccttacaat aatcaaccac ttccttttga tagcaacctg gggctaatcg aatctgatta
    29341 attgctggag ttattttagc tgtctcttga agagcttcta gatgatgcac cataaaattg
    29401 ctgacgccaa tgcttttaat aagccctgct tcaaccgcct cttccatata ttgccatgct
    29461 tgggcatttg cctctttcca agtgttacgc agagcttttg ggtttggcca atggataaga
    29521 tagagatcca cataatccag accaagacgg tcaatagaag cagctaaagc gtcttttgcc
    29581 ccttcataac tatgggcgtc attccagagt ttagttgtaa taaataagtc ttctcgtaga
    29641 accccagaat ccttgatggc acgacccaca ctttcttcat ttttataaat agctgcagta
    29701 tcaatatgac gatagccagc cttgatggct gctaaggtag attggtaagc ttcctcacca
    29761 tcggctgctt ggtaagttcc aaatcctaaa acgggaatct cataaccact tgtcattttt
    29821 actgttgtta ccatcacaaa aacctccttt tatcaatctc tgtaagcgat tactgtaatc
    29881 tattctatca taaaaaaaga aaatacaaaa agaattggcc tgccagacat agctacccca
    29941 atccttttgt atctcttagg catcaaaagc tttttgccca tttaaatagg ttgcaacaag
    30001 tgttaaatcc ttatcaagaa caataaagtc agccgcatgc ccagctttga tttgaccaca
    30061 tacgtcgtca ataccgacag aaacagctgg cacataagtt gccatatgaa tggcttctgc
    30121 cggagttgcg attccccagg ccactacatt tctaaggccg tcttttaatt tcaaaataga
    30181 tcctgccaag tttccactct ctttaagacg agcagtgcca tttgccacaa ccacagagaa
    30241 ttcccccaag aggtaatcac catctggtga accacctgct cgcatacagt ccgtaatcat
    30301 ggctacatga tcatgccctt tttgctgcat caaaatatca caagcaactg gtgacacatg
    30361 gtggccatca caaatcaact ctgcgtaagt atttggaaga ttataaaccg ctcccaccat
    30421 tcctggctca cggtgggtca atcctcgcat accattatag gcatgaaccc aaacgctagc
    30481 acctgcttgg acagcttctt tagcctcttg ataggttcca tttgagtgtc ctaaagctac
    30541 tgtgacacct tgtttggtca ctgtggagac aaattctgcg acaccatcac gttcgggagc
    30601 aagggcaatt tttttgatga gccctttagc agctttttgc caagcatcaa attcctccaa
    30661 gcgtgggttt ttcatataac ttgggttttg agcaccctta tattcttcgg taaaataagg
    30721 accttcaaag taaatgccct gaatcttagc ccctttaact tgatcagcga ctgaagcaat
    30781 agtccctgat actttttcta actgctcaaa ggtagaagtt aaggtcgtcg gtaaaaaaga
    30841 cgttactcct gttgctaata gtccttcact catttgatga atcccttggg cagaattatc
    30901 catgacatca gctcccgcat agccatggat atgagtatcc acaagacctg gtgcaatttg
    30961 ataaccggtg tagtcaatga tttgcgcatc tgccggaaca atttctgtcc actccccaaa
    31021 aacaccatca tgaagactta ggtagccagc cggtttcacc tctgttgggt aataaaaaca
    31081 atctgcttta agatagcatg tcatcttttt tcctcccttt tttccttatt atacgcctta
    31141 ttctttactt tgtaaatggt atataccact ttttggattc aaaaaagaaa acgtggatga
    31201 tcattcacgt tttctctttt tttaaagctg gtgtgaaagt actttctctg ctaaattcaa
    31261 ggcatgatct gtgatgcggg tatagtggga aataatatca ataaaattaa tacctgcttg
    31321 tgccgtacat tctccacaat tcaagcgctt aatgtgggtt ttacgtaatc gtcgctcttt
    31381 ttcttcaatt tctttatggc gagtcacaat agtttgcgcc aagtctgtat cactatcaac
    31441 gatagctcta atggcatcca gtgttaaaca atgcgtcaat tgatacatct ctgtcaattc
    31501 ttgacgagca gatattgaaa aaccaatttg tttagatatg atgccttcaa ttagaatacc
    31561 taaagactca gaatgatctc caattctttc caaatcacgt gaagagtcaa gaataccagc
    31621 caagacttca ttttcacttg ggctgagggc ttcgttagaa atatcaacca aataagtggt
    31681 taactcctca tcgatggtat caactgctct ttcataacgc ttgacctttt ccccaaattt
    31741 atcgtcagct gtcataatat aactgtaaga agcttcaaaa gcttgaatcg cataagaggc
    31801 taaatgaacc aattccttgt gggcatttcc tagtgcgata gaaggggctt gggtaataag
    31861 taagcgatct aaataaaggg cttcgtattt gaccacttca tcttctcctg gaatgagtct
    31921 agtcacaatc atagctagta gactaatgaa aggaattaat aagatggtat tagtgatatt
    31981 aaaagagccg tgagaaaaag cgatagtcat ctcaggtgtt aaactcaact ttgattgtag
    32041 ccataacatc aatgatgtga atgggactag gataatcata aaaataattg taccgatcaa
    32101 attaaataac acatgagctg ctgccacacg tttagcagca atatttgaac ctatagctgc
    32161 taagacagct gtaatgcagg ttcctatatt tgagcctaac aaaataggaa tagccccctg
    32221 taaggtcaaa agaccgcctg aaaacagacc ctgtaaaatc ccgataatgg ctgccgatga
    32281 ctgaatcaac atggtcaaag ccgttccgat aaagacacct tggaagggtt tatcacctaa
    32341 tgtcgctaaa taattttgaa aggctgacac agatttcaaa ggatccatgg catctcccat
    32401 taggttcagc gagaagaaaa ttcctccgac accaaaaatg atacgcccaa aattgttgag
    32461 ctttttatta gaggtaaaga aaagacaggc tgcaccaata aaaatcatgg gtaaggcgta
    32521 atctcctagt ttaaaaccaa ttaaaaacga agtgatagtc gttccaatat tagcccccat
    32581 aacaattcca atggcttgtc gcaaatttag caaacccgca gaaaccaaac caaccgtaat
    32641 cacagttaca cctgaactgg actgaattaa ggcagacatg gcaattccga ctaagatacc
    32701 aaaaaaggga ttactggtgt atttatcaat gtaatacctt agtttatctc ctgctgcttg
    32761 ttgcagacca tctcccatat acttgatact gaaaagaaag agacctaagc ctccaaagaa
    32821 atgaaatgca atgtcttgcc aatttactga cacaatattg tcctcgctca attttttagt
    32881 ctattctatt ttaatgtatt acggtcttta tcacaaggtt tttttaagcg tttctgtgta
    32941 taccgatgtc aacatttgca tattattggt ttgataaggt gttaggcttt tccgatattt
    33001 tacatcattt gtttttgcac aaatcttttc atttcgttta attagaggta taatatggct
    33061 acgatgaaac aatatcataa aggagattcc atgactaaat cacaaaaaga ggcgctttat
    33121 tggatgttga gtgttctaac tatcactcta ataggtggct cctgccttat ttttggatcc
    33181 caccctcaaa cgcaagataa ggtagctaaa cattctaaaa gtgctgctag tttactaaaa
    33241 aaagcagtta aagcagttaa tgacgctgat cgtttagcca ctgcagctgc tatccaagaa
    33301 gcccagaaag ctgttgataa attagcagaa tcttctaaga aaaaaacgct acaagaacag
    33361 ctaaacgttg ccaaagcaaa gcaagagcaa gaagatgcag ctactcaagc agttaaagct
    33421 gctgaggaga ctctcaatca aaatcttaaa gatatcgccc aaaaagcagt taacgactta
    33481 agtaacaaag gtaaaaaagc cgctttacaa tctcggttag acgctatttt accagcgaaa
    33541 cccattattg atgaatttcc aagacaaagt ggagaaataa cagataactc ttactggaca
    33601 cctttcccag gagacgtctc agatacttat gataattccc agtcacctac tttagaccca
    33661 agctcagaat cttcagcttc agatgttacg ccacaaccta gtcatcctga tccaattcca
    33721 cctcagacct cttcagaacc ttccgactca ggagacaagc aatcatcaaa agagtaatac
    33781 aaaagttttt cgtaaaatca atcaataaac gttttagacg actatcttag tgattagtct
    33841 tcctgacatc ttaaaaaagc tagctcaaat caaaagagtc tagctttttg ttttgttaga
    33901 tagcattaaa tttcgtagcc cattaaaagt cctccgtctt cagcataaaa gctacacaaa
    33961 cctgaggtcc ttccagtttg aatcacagca tcaggatata aagatttgat tttttcactg
    34021 atctgctcac aaatttttgg attttgagca tgagcaatgt aaacttttcc gcctgcataa
    34081 ccctcttttt gaatttcttc tatcaaagca gaaactgctt ttttttgacc acgcgctttt
    34141 tgaagaagtt caagcgttcc tttattgcta gctttcccaa ccattctaat attgagtaag
    34201 ccaattactt tacctactaa tttactcagt cgaccatttt taaccaaatt atcaaccttg
    34261 gcaagcacaa aaatcaaacg tgttttttgc tgataagctg tgatctgttt gacaacttct
    34321 tcaaaggata atccaagatt gatcaaccgc tcaagctcca gtacgagcaa atccatctcg
    34381 ccacctgctg ataaagaatc gattaggtga atattgacat taggattttc ttcaagaagt
    34441 tcatttttag ccagtcgtgc actattatga ctgccagata aggtccctgt aagtgtcatc
    34501 acgataacat tatctgcacc tctataggcc tgcaaaaagg cttctgggct tggacagcta
    34561 gatgtcgttg ctttagacga ctggtacata gttgtcatca tattatcgat atctaatcca
    34621 tcatcatctc taaaaatttc tgtgccaatc tgtagggtca aaggcacacg ttcaaagcgc
    34681 aactctttgg attgtctagt caaacttcta agatcacatc ctgaatctgt tacaattttc
    34741 caagtcattt tttatccttc tttttctcta atttgtgttc agtaacaaag accagattat
    34801 taagccttcg ctaagatgtc taggctaatg gtaataacta gtcattaaat cctatgtttt
    34861 attactagcc ttttcaatag ttagtaaagt catccaatag agctatacaa ggaggtaatg
    34921 aggctcctga taactaactt tagttgacaa actaagactt ttcccataaa attatatcat
    34981 tattaagtcg gtttttgcta ctgataactc atcagatgta acacttgaaa ggtatttgtg
    35041 atgactgata aagcactctc aaaaagatca cttcaaaact taacacaatt taataaagaa
    35101 acacgacgca tagcacgtga atctatggaa atcgccttac taaatcttct agaaacaaaa
    35161 cctttaggtg atattacgat ttcagaattg gtgacgaaag caggagtatc tcgtaacgct
    35221 ttttatcgta attatacctc taaagaagcc attatagaac aactgttagt aggggttatc
    35281 cgtcgtattt ttcgtggact aaaacaattt gatattaaaa ctcaggctta tcaagcctgg
    35341 ctctatcttt ttactgaagc taaaaaagaa gctcagcttc ttaaaatgat tttcaaacat
    35401 cacctgcacc atcttctaac acgattagtc actaaacgtc tgaaagctta tcaaaaatta
    35461 aaagacaaaa aacaatcgca ctataagcgc cttttttgga gcaatgctat tgtttctgtc
    35521 ttgacaaact ggatttcaga tgacatgatc gttcctgccg aagaaatggc tgctatgggc
    35581 ttgcctctat taacgtaaaa aggttccttt tgggaacctt ttttatataa tagttagtct
    35641 ttttgagctg caagtgcaaa ctgacggata gcctgagcca cacccgccat atcattagtc
    35701 aaagtgactt tgtcagctaa tggcttgata gctgctgaag cattttccat agcaactcct
    35761 aaaccagcat aagttaacat ctcaatgtca ttgggagcat ccccaatagc catcacttgg
    35821 tcagcagtta aacccaaatc ttctactaat tctttgagag ccgatgcctt gctgactccc
    35881 tgagggagaa tttctaaaat attatcttgg ctacgaacaa catggaaact ctgactaagc
    35941 tgatttctca ccgcacgttc aaaggcatct agcgcagctt tttctcctaa gtacatggct
    36001 tgaaaaatca ggcgaggagt gtccgacagc tctgctaagg agacaggctt gacaatggta
    36061 aataccaaat ctccatcttc ctgcacaagg tctggcactt cttcttcaag caccaaataa
    36121 tcgttttcct cagttaaagt caggtaaata tctggaaaac tttggcttag ctcttccagc
    36181 aattcaatat ctgaaacctt caacattttt gaatggcgta attgccagtc aggactacta
    36241 taagtactac agccattatt gataatgagg aattcctctt gtgtcaaacc taattggtca
    36301 aaatagggtc ttgtccctga ctgaggccgg cctgtacata agacaatttt taagcctgac
    36361 tgagctgctt cttggatggc tgtgatattc tcttttggaa tctgtttgtc ctggtttaat
    36421 aaggtgccgt ccaaatcaat tgcaattagt tgaatcatcc tatctttcct ctttccattt
    36481 gattttaggg aggttccatt ttttacggtt gccaatcatg cgtaaagtcg tgagtaaaac
    36541 aaccaaggca taacattcaa tcgttgaatc tgctagttta aaatgcaaaa caatagctgc
    36601 aagaatagac cacccagcat agatttcact acgcaaaaca ctaggttttc gccctgccag
    36661 aatatctctg acaacaccac cgcctgcacc agtcaacaca gctgctacaa tgacagcact
    36721 taaaggttgg ttaagacgga cagcatgcaa ggctccttgg acactaaaag ctgccagacc
    36781 aatggcatca gtcaaaacag ctgcccgtac ccaacctcta gccattagct taggaaaaag
    36841 cataatcaaa accatagcaa agaaagcgca agtgaattct ggcttttgcc cccataaggc
    36901 ctctataggt aaaccaatta gggtgtttcg aatggctccg cctccaaagg ctgtcacgaa
    36961 acctaaaata aaaatcccta aaatatcaaa ttcttcttcc atggcaacaa tagcaccaga
    37021 aagagcaaag gcaatggtcc cgatgatatt taaaatttcc cacatatcaa ttgtcataag
    37081 ctaccctatt gtcttttagt cttcttttag tttaccacgt tttcctttaa aaaataagta
    37141 gaaaaagata aaatgtaagg ataaaaaagc aaggataaaa cccattttca tccttgctac
    37201 aaaagattca aacaattatc cccatactta tcttatcaca tcaatgagag ttgcttgata
    37261 ggataaggtt gttttagatt ttttccgtcc aagggctagc ggtctcttcg agtaccctgt
    37321 ttaactcgtc aatattttgg aagccttcag tgcatagcca ctttcttgct tcgtcctctc
    37381 ctttagtaat ataaactgga acggatcctg cccacgtggc acgcccacac aaaacaccgc
    37441 taaattgagc acctgctgct gctgcaaagt ataatgtctc ttgaaaaagt ttggcagata
    37501 cgcctgcact caaatagata taagggaggt ggctggcagc ttcttggtct tgaaaagctt
    37561 taatagcctc agcttgactg taatggactt cctcttcagt aaaaccttct acataagcca
    37621 tattaacagg aacctctact tttagcacat caacgccaaa acgcttatcc gaaaatacta
    37681 acatagcacc gttaacctta tgtggcttaa gctttgcgta tgccgcactg ttgttatctg
    37741 atattctctc atcataactc aaaagttcaa gaaaaaaagg gatgtcttca gctgtacatt
    37801 ctgaaccaat gcgttcaata taagcttgtt tttgtaaatt gatttgttca tctccatcaa
    37861 catcataata gagcaaaaat ttaatagcat ctgcacctgc tgctttcaac cgtttgaccg
    37921 accattccac taagcaatcg ggtaaacggc tagtggttgt agcatcataa cctgtttttt
    37981 cgtaagctaa cagtaagcct gtctgattag cttttacctt agtagccagc aacccatatt
    38041 ctggatctaa taaaatagat gaagcataag gcgtcaattc ctcagaaacg agaactttca
    38101 atgtttctat ctgtgtaact gtcgcttctc cttcttgatg tgctgccatc atttgcttca
    38161 gtgcacctcg ttgatcaaaa gctaaagctg atatgattcc ttgatggctc actttttcta
    38221 agtaatgtct tttatttgct gtgattgtca tgtgatacct ctcttacttg aagttgctga
    38281 tataattgtt taaaagttgc catagtcatc aaatcttgat aatagtgagt agctagtctc
    38341 ttaggcagac tgcctgagaa gttaagacat caaacggctt agcaatagta ctcatgtggc
    38401 tcaaagagac ttcggcttcg tccacatcac tgtagggacc agcttccaaa atttttgtct
    38461 gcatgccttc atgtaaaaca gcaatacaat ttctcgtttc ccctttgatt ttgaaaaatt
    38521 ggttagaaat gccctgttct tgtaactgat gaatgataaa gtcacccagt ttactaccac
    38581 taaatcctgt tgcaataact gaccagcttt tgttaacatt ctggtcacat ttagctcctt
    38641 atcaccagct gtttttgccg ttctgtcaac acgattaacc gtgtccatag ccagacaatc
    38701 taaaggatag gaaatagcga cagaaggatt taaggtaact gttaaactca tctcttcttc
    38761 ctaatcgtga tactcaccac ggtcccattt ttctaaaaat tctgtaaaga aatcaggatc
    38821 ttcttggtta gcatgatgag tttccaagtg ggctatttta gcaattagct tcttattctc
    38881 ttcagtttct ttgtattctg ccttgataaa agcgtctata atatcacaca taagaagttc
    38941 accagtgatt ttaccaccaa agccaatcac attggcattc aattcttcct tggcataaag
    39001 cgctgttgtc atatctcgaa caagggcgga gcgaatacct ggtaccttgt tcacggcatt
    39061 attaatgcca actccagttc cacaaataca aacacccaag tctgcttgac cactaaccac
    39121 agcttcgccg actttttttc caaaaatagg gtaatgggtt cttgtatggt cataagtgcc
    39181 acagtcaata acgtcatagc ctttagattt caaaaaatca gaaacagcca ttttttcgtt
    39241 ggtgacaata tggtcacaac caatcgcaat tttcatcact ttatcctcct ttaggccatt
    39301 ttattgagca tgtcaacacg aatttgatga cgaccaccat cgtagggacc agtcacaaaa
    39361 cctttgacga tattcttcgc taattctgga cctacaatct ctgcccctat tgtgatcatg
    39421 cgggcattgt tgtgaccgcg cgtcatgtaa gcagagcgtt catctgacac ttcagcagca
    39481 accattcctt ttaattttgt ggctaccata aagggacctg ctccgtaagc atccaccata
    39541 atgcctaaac gcccttctgc ttcattaacg gctttagcca ccgctaatgt gttatcaata
    39601 aaatcactgt taatatcagt cacgtcaata atgtcatagc tttcttcttg aagaaaactt
    39661 ttgatcaatt cttttagtgc atttccgtgt gcatctgcac ctaaaataat agccatcgtt
    39721 tctccttttc taatccaaac cgcttgccaa tctagagaag ggggacatga ttacctgtcc
    39781 ttagatcaag atgattgcca gcagtcttag ttgtcttcca ttatttcttt caagaaaagc
    39841 gcttgcgggt acccacagag cgccttgcct tacatgagga atgctctttt ctccctaggg
    39901 gataaggcag gaatacctta agcggttgtc tgctattccc aatagttttt tagttagtca
    39961 cctaacctga ttttatttca ttgttttcgc atactcagcg ttacgtgcct tcatttggtt
    40021 agcataccaa gcaaagagac ctaggtaaat ggccacaaag atgattagcg tgatgattga
    40081 ggcaatctga cctgttgtcg cttgaccaat cacataacca aaaatttttt ccattggtcc
    40141 ttcaagtgtt gaatgcgtaa tcaaggaacc agcgcttgta cctgctggaa aagcccctac
    40201 ttttttagcc aactctgtcg caaatggagc aatcatagtt cctgacaaga ggaataatgg
    40261 caataagagg gaaccaaaaa taatcatgcg aatcaaacga ccacgtgtga ccacaagaag
    40321 agctggtgtc atccccatag caataattcc cgccaatggt aaaatcccat tgccaacttt
    40381 tgataagaga acggcttcaa tcaacatgat tggggcaaga atattggcac aggcccagat
    40441 ttcagcgcga ccagcaatga aaggccagtc caaaccgata ttgaaacgac gtccatgcat
    40501 tctagcattg gcaaaattag taatcccttg tgacaaaggc tcaacagctg cgataaacca
    40561 agaaccaatc aaagagaaaa gttccaaaca ggcacccgcg gtaaagccta gaccaaacca
    40621 atttttgata gattcaacag tgaaatgagg atctcccata atcccaatca caaaacctag
    40681 agccattccg ataaagaact tagagcccca gaagccaatt tttttgttca atttagcagc
    40741 atcaaagtca tatttatcta gccccggaaa gaccttgtca aaaatcttat ctaagaccat
    40801 aatgattgga ttcatcatgt agttcatgtg agttgaagtc attggtgatt gagaccctgt
    40861 cccaagaagg tcatcaaagg tcggtttcat caaatcagag ttaatgattt tcaatacccc
    40921 aacaagaata actgccacag tagcaatcaa aagagacaca aagactggta aatggttgcg
    40981 agccgcatac cacataatca atagacctgt gatagataaa tgccaaatat caaaaatatc
    41041 gacgtcaagg gtgtccgttt ttttcatgac aatcatgaca atattaacca ccagcatcac
    41101 cagcaagaaa taaagggtgt aaggggaacc ccaggtaatt gttgcaagcg gtgcccaacc
    41161 gacatcaata atgtttaaac tgatccctgt atttttaaca aaggcctgta aagactctga
    41221 aaaagccccc gtcaaaatcc caataatggc tccaatacct gtaagagcga tagctaactt
    41281 aatcccgcct tccagtgcct ttgtgaattt aaccccgaaa atcatggcta aacctgtcat
    41341 gacaatcagc atgaccacac ctgatcccaa ctgaatagga aatcccagaa tcttgttaat
    41401 aatgtctaaa aaaggttgca tcatcttgtc tccttatgtt tttattaact cactgcaaat
    41461 ggttttcttt aatcacgcgc tctaattcag caaaaacagg ctcactcatg attgggatac
    41521 gatagagaat cggtcctgct tctactgtgg gaattggcat ctcaaatccc aagtcagttt
    41581 ttgcaatcgg cgtaaaaata tcataacgag acaccaattc ttctgtgatg tctttgacca
    41641 tcaccgcatc acaatgcaca tcatagccac gatcagtcat ttggctttcg atagcatctt
    41701 taatttgatg gcttgagtta acacctgcac cgcaggctgc tagaatttta atcatgctga
    41761 aacactcctt tttctattaa atagtttgaa atagcttgtt tatcatcttt tagactggct
    41821 aaatagctca gattgccttt cgtaataaag aaggtaatga gattggatag aatcgtcgtt
    41881 tgaccctcgt tttgactatt gataataaag aagaaatccg ttaccaaaca gtcttcctct
    41941 ggattaatca tgtgcttaaa ggtaagggct tgactatttc taacatagac cacttgatca
    42001 accaagcaat acttggtttc tgtatgcgga atagccgcat acaaaatatc tgacccatct
    42061 ttgaagtcca ctttgagtcc tgtcggaaat accgcctcac gctccactaa agcctgatgg
    42121 tactcttgcg tcacataccc aaccttttcc aagtggctag caacaagatc aaaaagctct
    42181 ggctgcgtcc ttgcttcagt aaatagaata ttaggaaaca ctccctaccg cctttctata
    42241 agtatcaatt tttaacaact gtcatctcat catcaacaaa ttggtaaccg tttacaaaat
    42301 cattatagta taacctttaa cataattcaa catttttacg caatattttt tgttatatat
    42361 gtttgttttt gttttgttta ttttgtttta ttgaaatatg aaggtctata tgctatgata
    42421 aacataagta aataaattat agaaaggagt cccttagatg ttaaaaagag aaagacttct
    42481 aaaaattacc gaaatagtca atgagcaagg tattgttact gtgaatgata ttattcagac
    42541 tttaaacgtt tctgatatga cagttcgtag agacttagat gaactggaaa aagccggcaa
    42601 attgatccgc atccatggag gtgcacaaag tattaccatg cctaataaaa aagagcggtc
    42661 aaatattgaa aaacaaaccg ttcaaacaaa ggaaaaatgg gaactagctt catatgcgac
    42721 acaactagtc aatgatggtg aaacaatctt tattggtcca ggaacaactt tagaatgttt
    42781 cgctgagcaa ttaaaaaatc gtcagattcg cattgtgacc aatagcctcc ctgtctttaa
    42841 catccttcag gacagcgaaa ccattgacct gattcttatc ggtggtgaat accgatctat
    42901 tacaggagct tttgtagggt cccttgccag tcaaaatatc tcatccttaa aatttgccaa
    42961 ggcttttatt agctgtaatg gcatttataa aaacgatatt gcaacttata gcgaaactga
    43021 aggagagatt caaaagctag cttttaataa ttctattgaa aaatacctct tggtagataa
    43081 ccaaaaattc aatgcctacg acttcttcat tttttaccat ttaaacaaca ttgatgctgt
    43141 tgttacagat tcgcaaataa ctgaggacgt catcgaacgt tatagtcagt tcacacaatt
    43201 attagtagcc cacgaaaaca aggagtaacc gtttcatcaa gaatggacag cctgtcagcg
    43261 catcgtcctt aaacaccttc tggcaaaagt gatttgcccc cagagaggtg tttttgtttt
    43321 gctcggaaca cacctgctaa cgtcagagtc tttatgagtg accacaaaaa aagaagccca
    43381 gattaaaaaa ctggaacctc ttttgagatg aagtaagact accaaaataa cctaagacca
    43441 tttatgtaat tcaaatttgg tatccttcaa agcacgcttg agtaggaacg ttaaaggacg
    43501 tcctgtcacc ttagcctctc ctttttccaa atttacctgg accgattgca caccggggac
    43561 agctgataat ttttcagtaa ccgttcttgc acaaccgtca catgtcattc ctgtaacctg
    43621 ataatgtttt tccattttgt attctccttt aaatggtttt tccttttaaa cgcaatgcat
    43681 tcaaaacaac tgatactgaa ctaaaactca tggcaaaacc tgctaacatc gggttcaata
    43741 atgggcctcc aaaaagatga agtaagccca tggccactgg aatcatgagg acattgtata
    43801 tgaatgccca aaataaattt tccttgacaa tcctcatagt cgctcgactc aatgacaggg
    43861 ctttcaccaa atccagcata tcaggtttca tcagaatcac atccgccgat tcaatggcaa
    43921 tatctgtccc tgaccccata gctatcccaa tgtctgctac agccaatgcc ggagcatcat
    43981 taataccatc acctaccatt gcaacttttc tcccttgaga ttggaggttt gttatcacgc
    44041 ctgctttttg atcaggtaag acttgactaa tcacatctgt aatccccact tgagaggcaa
    44101 tagccttagc ggtagcatcg tggtcacctg ttagcatgat agtgtgaatt cccagttgat
    44161 gcaaagcttc gacggtagct tgactatctt ctttgacagc gtcagcaata ctgaaaagag
    44221 ctcgtagctg cccctcttta gcataatata caatggtttg gccttttcct gaagccttag
    44281 cacaaactga ctgaacagaa gctaaatcaa taccaagggt tgccattaaa gtagcatttc
    44341 caatcgccaa acgacgccct tcaagctgac cagtgactcc taggccggtt aaagactcaa
    44401 actcttccac tggtagacta tcataaccag cttcctgagc agcacggaca atagcctccc
    44461 ctaaggggtg ttgagaatag gcctccaagg aagcagcttc ttgcaagatg aggtgttcat
    44521 ctcccgaaaa agttaggagc ttttgaagac ttggtttccc ctccgtaatc gtccccgttt
    44581 tatcaaaaac aaccgtatct acctgatggg cttgttctaa gatgtcacca cctttaaaaa
    44641 gaataccatt ttcagctgca cgccctgttc caaccataat cgctgtcggg gttgctaaac
    44701 caagcgcaca aggacaagca atgactagaa cagcgatggc tgttgtcaaa ctaaaacgaa
    44761 gactttcctg catgaatacc aaccaaaaca cccctgttaa cagagttaat accaaaacca
    44821 ccggaacaaa gacacctgag actctatcag caatagctgc aataggtgct ttattttctt
    44881 gagcattctc aactagttgg ataatttgtg ctaacaaggt atcattccca agcttatctg
    44941 gtttaatcgt gagggcgcct ttcccattca agctgcccgc aaagacatta tctttttctc
    45001 ccttttcaac aggaaaactt tcccctgtta aaaaggattc atccaaactg ctatgtcctt
    45061 ccacgataat accgtcaata gaaacttttt cacctggttg aaccaggaca aggtcatcat
    45121 agctaacctc ttctaacggc aacaattgcc attggctgtt tctccatacc ctcacaacag
    45181 tagccttcaa ggataagagc ttttgaatag cttgggaagt acggccttta gaacgacttt
    45241 caaaatactt tcctagacca atgagtgtca aaatcactac caccgattca aaataaaggg
    45301 agtgcacaaa gtgcgcctgg cccatactga tttcaaccac tccataaaga ctataagcaa
    45361 aagcaaagct ggttgctaaa gctaccaagg agtccatatt aggatatccc ttaaacaaag
    45421 ctttaaaccc attatggtaa taatgacgat ttaatactaa gactggaatc gtcaagagta
    45481 gctgactaaa gctgtaaacg aaaggatagc gatctggttg cagaaatgaa ggaagccata
    45541 agcccaccat gtgacccata gctaggtata atataggaat ggtgaatagg gcagaccaca
    45601 agacctgaga ccaaataacc tttaggtctt cgtcacgacg acctgtttgc atgtgttttg
    45661 catgttcatc aaagacttgt gcaccataac cagcagcttt taccgcttga ataactttgg
    45721 ccgtttctaa atcgtcatct tgataagtaa ccgtgagttt ctcggtcgct aaattcacaa
    45781 cagcctgttc tacttcaggc aattgattga ccgttttttc aattgttaga gcacaagagg
    45841 cacaactcat tccctccact aaaaatatct ctttagccat taatacgtct cctttttctg
    45901 acagtggcac tgcccaggca ggcaattaca cggcacctca agaactgcat tttctttttt
    45961 gacttctagt agctcttgga atgcctcaat ttcctctaag gtcatcggta agtcataaag
    46021 ccgttcaaga agtagacgcg tgtgttgccg ctgacaaata ccgtctaaaa cagtcgctag
    46081 tgcttctttt tgtagcaaag tctcatccaa aagagcttga taaatataag ctcttccttg
    46141 ccgataagac gttaaaagat tttttttaac tagcctacca atgagggtct taatagtgga
    46201 atctgaccat tgatattttt tccttaaaat agtaataata tcgctacttt taatatcacc
    46261 actggcccac accacacgca tgacttccca ttcagcatct gaaatatttt gattcatcaa
    46321 ctcacaacct tttaatctac atttgtagat atattatatc atatttgtct acatttgtaa
    46381 atatataaag tttctaatga t
//
LOCUS       DAUELZ010000003        46022 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.003, whole genome shotgun sequence.
ACCESSION   DAUELZ010000003 DAUELZ010000000
VERSION     DAUELZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46022
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.003"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            67..2265
                     /gene="nrdD"
                     /locus_tag="VHI09_000088"
     CDS             67..2265
                     /gene="nrdD"
                     /locus_tag="VHI09_000088"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEQ6120001.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIVKAMKQACEDWSDGYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            2449..2595
                     /locus_tag="VHI09_000089"
     CDS             2449..2595
                     /locus_tag="VHI09_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120002.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRLQELKARFLEKSK
                     KQDN"
     gene            2624..3559
                     /locus_tag="VHI09_000090"
     CDS             2624..3559
                     /locus_tag="VHI09_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ6120003.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKTNLDGIFIHAATSAHAELASLFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKKLSSLSTKRKVAVEKNDLNRPGDMAFKLFDF
                     FIHPLDTALFLTEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            3568..4062
                     /locus_tag="VHI09_000091"
     CDS             3568..4062
                     /locus_tag="VHI09_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ6120004.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLSSQGVPAIQYVAFDERSQAIGFLNLRLRLNERLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSCAISKNITTILVTCDETNVASRAVIVANGGVLEDSRGGTERY
                     WIEE"
     gene            4077..4691
                     /gene="nrdG"
                     /locus_tag="VHI09_000092"
     CDS             4077..4691
                     /gene="nrdG"
                     /locus_tag="VHI09_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEQ6120005.1"
                     /translation="MAEKCWNNPKPKQWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRCELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(4865..5596)
                     /gene="yaaA"
                     /locus_tag="VHI09_000093"
     CDS             complement(4865..5596)
                     /gene="yaaA"
                     /locus_tag="VHI09_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEQ6120006.1"
                     /translation="MLTFLIPTAKEMVIPKESYPHLLPQPSQAILKAMAAMTTEDLAK
                     AYRIKEEAAKKEQQRWQDMTSQQSLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWIGPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(5731..6486)
                     /locus_tag="VHI09_000094"
     CDS             complement(5731..6486)
                     /locus_tag="VHI09_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ6120007.1"
                     /translation="MTYPYNSTISLGKVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTAFLVPDRHSLGLHFLTRGISLEGAADHGY
                     SEAIYLSDPEGNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKHFLL
                     AQDTRISHVHLSVKNALDSSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHWSAP
                     YLKKHQEGTPGLAFLTIHIETPLLFSATLKKAR"
     gene            6672..7010
                     /locus_tag="VHI09_000095"
     CDS             6672..7010
                     /locus_tag="VHI09_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6120008.1"
                     /translation="MLKKRPACPVETTLSVIGNKWELLILRDLLKGTLRFGQLKSSIG
                     SVSQKVLTAQLRAMEADGLVHRELYAEVPPRVEYSLTETGLSLAPVIEAMSDWDQTYQ
                     EKHADLFEEG"
     gene            complement(7289..8002)
                     /gene="treR"
                     /locus_tag="VHI09_000096"
     CDS             complement(7289..8002)
                     /gene="treR"
                     /locus_tag="VHI09_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEQ6120009.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIKHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFARRRPK"
     gene            8213..10237
                     /gene="treP"
                     /locus_tag="VHI09_000097"
     CDS             8213..10237
                     /gene="treP"
                     /locus_tag="VHI09_000097"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEQ6120010.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QRVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWIIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANSIG
                     VGGLPGFMAINVKYMIPFFICMAVAIVVPMFLTFFFRKSHIMTKTEDEAKLPETPVSE
                     APAATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGALLQPTEGVLVAP
                     CDAEVSVLFPTKHAICLVTTEGLELLMHIGMDTVNLAGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDAFTVTVEGNLPHQIKANDKLVVAMKK"
     gene            10305..11933
                     /gene="treC"
                     /locus_tag="VHI09_000098"
     CDS             10305..11933
                     /gene="treC"
                     /locus_tag="VHI09_000098"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEQ6120011.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTTVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMSQGNGWNALFYNNHDQPRALNRFVDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAK
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVSKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYADEVELELPPR
                     YQHGQVLISNYEKVSICEKVILKPYQTLAILADN"
     gene            12141..14036
                     /gene="pepO"
                     /locus_tag="VHI09_000099"
     CDS             12141..14036
                     /gene="pepO"
                     /locus_tag="VHI09_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEQ6120012.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGDHVPDDAILANFVKFHRLVADYAKRDEVGVSPILPLIEEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYTGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDESLSLVENAQNLAKITIAHTWSKWNKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQEPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKVNGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNVTLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(14202..15242)
                     /locus_tag="VHI09_000100"
     CDS             complement(14202..15242)
                     /locus_tag="VHI09_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEQ6120013.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(15376..16143)
                     /gene="rpsB"
                     /locus_tag="VHI09_000101"
     CDS             complement(15376..16143)
                     /gene="rpsB"
                     /locus_tag="VHI09_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEQ6120014.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADTQADSIEEIVEVVEGDNA"
     gene            16447..19521
                     /locus_tag="VHI09_000102"
     CDS             16447..19521
                     /locus_tag="VHI09_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ6120015.1"
                     /translation="MTKGIRFQLLGSPHIFLDNKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLNKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLAYINSQLGSQPVTEEEMEHLYQASQGNPFFLSEYTHALLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDKIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKHIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVAY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLEIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIRQGA
                     TLSQQEKEMADHLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(19552..20538)
                     /locus_tag="VHI09_000103"
     CDS             complement(19552..20538)
                     /locus_tag="VHI09_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEQ6120016.1"
                     /translation="MLEDYYPSTTSYYHSGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSSSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPN
                     LAVLVLQHIAASGKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHN"
     gene            complement(20725..22266)
                     /gene="hutH"
                     /locus_tag="VHI09_000104"
     CDS             complement(20725..22266)
                     /gene="hutH"
                     /locus_tag="VHI09_000104"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEQ6120017.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGIACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKGVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLIEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KSENHELGKGTKVAYDLFRKEVNFIEHDKHIEIYDELNKASTVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(22277..23620)
                     /locus_tag="VHI09_000105"
     CDS             complement(22277..23620)
                     /locus_tag="VHI09_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEQ6120018.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRDPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNIGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFENLAKLSVIFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPKEQI"
     gene            complement(23840..24436)
                     /locus_tag="VHI09_000106"
     CDS             complement(23840..24436)
                     /locus_tag="VHI09_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEQ6120019.1"
                     /translation="MTKVTIKAPSDYLQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     KSTVALRQTTFTLLDGYHRILMTLDKPITLSDLSHAKEISLTPFEPYAFEGDTRIVSR
                     GICQDMNLMYDDQYQGQLLPVWSDTLFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCLNQTSPAQTPVAVWAGLWYKT"
     gene            complement(24458..26131)
                     /locus_tag="VHI09_000107"
     CDS             complement(24458..26131)
                     /locus_tag="VHI09_000107"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ6120020.1"
                     /translation="MVLSDIEIANSVSMEPISKVADQLGIDKEALCLYGKYKAKINAR
                     QLVALKDKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVQKTDLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACNKRGVDVVISDVWANGGAGGRELAEKVVTLAEQDNQFRFVYEEDDSIETKLT
                     KIVTKVYGGKGITLSPAAKRELADLERLGFGNYPICMAKTQYSFSDDAKTLGAPTDFT
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(26149..26775)
                     /locus_tag="VHI09_000108"
     CDS             complement(26149..26775)
                     /locus_tag="VHI09_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEQ6120021.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPDPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQDIITEVHAKSTALQASLLAAIDKDTEAFNLVSAVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     GAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(26786..27685)
                     /gene="ftcD"
                     /locus_tag="VHI09_000109"
     CDS             complement(26786..27685)
                     /gene="ftcD"
                     /locus_tag="VHI09_000109"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEQ6120022.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELGIPIFLYEDSATRPERQNLAKVRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEVIGLAPAKALIDV
                     AEYYLQVEDFDYHKQVLENHLLG"
     gene            complement(27773..29803)
                     /locus_tag="VHI09_000110"
     CDS             complement(27773..29803)
                     /locus_tag="VHI09_000110"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEQ6120023.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFEEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRFRPKDRIYGKPIDEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFKSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIETRHSQGWVSQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNIYDGGYCPAGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTIALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVISAVDQLFH"
     gene            30095..31360
                     /locus_tag="VHI09_000111"
     CDS             30095..31360
                     /locus_tag="VHI09_000111"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEQ6120024.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDS
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITDDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(31418..32950)
                     /gene="ahpF"
                     /locus_tag="VHI09_000112"
     CDS             complement(31418..32950)
                     /gene="ahpF"
                     /locus_tag="VHI09_000112"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEQ6120025.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHGSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     AKTNNMTIIKNVSTKDIVGEDHVTGLNYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(32971..33531)
                     /gene="ahpC"
                     /locus_tag="VHI09_000113"
     CDS             complement(32971..33531)
                     /gene="ahpC"
                     /locus_tag="VHI09_000113"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEQ6120026.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQKQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            33937..34007
                     /locus_tag="VHI09_000114"
     tRNA            33937..34007
                     /locus_tag="VHI09_000114"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33969..33971,aa:Cys,seq:gca)
     gene            34515..34718
                     /locus_tag="VHI09_000115"
     CDS             34515..34718
                     /locus_tag="VHI09_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEQ6120027.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            34914..35375
                     /locus_tag="VHI09_000116"
     CDS             34914..35375
                     /locus_tag="VHI09_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEQ6120028.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            35375..37819
                     /locus_tag="VHI09_000117"
     CDS             35375..37819
                     /locus_tag="VHI09_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ6120029.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHYLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDSGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKLAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKDIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVRPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSHGKLTFHIA"
     gene            37997..38287
                     /gene="groES"
                     /locus_tag="VHI09_000118"
     CDS             37997..38287
                     /gene="groES"
                     /locus_tag="VHI09_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEQ6120030.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            38323..39954
                     /gene="groL"
                     /locus_tag="VHI09_000119"
     CDS             38323..39954
                     /gene="groL"
                     /locus_tag="VHI09_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEQ6120031.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            40205..40471
                     /locus_tag="VHI09_000120"
     CDS             40205..40471
                     /locus_tag="VHI09_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6120032.1"
                     /translation="MNHFGEIFKTFRESKGSRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMPIDEFMYAVHDFHRDDLNELLSKSEAFRNNSR"
     gene            complement(41057..42556)
                     /locus_tag="VHI09_000121"
     CDS             complement(41057..42556)
                     /locus_tag="VHI09_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ6120033.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDPSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVAKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQEKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVKEKPKNKKGLNRS"
     gene            42803..44443
                     /locus_tag="VHI09_000122"
     CDS             42803..44443
                     /locus_tag="VHI09_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120034.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DIAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDNSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDQKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALAPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(44532..45533)
                     /locus_tag="VHI09_000123"
     CDS             complement(44532..45533)
                     /locus_tag="VHI09_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ6120035.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKHPLTQEDICVQAKSATFDAVLNAQKARK"
BASE COUNT        13490 a         8706 c        10153 g        13673 t
ORIGIN      
        1 aaacttgaca caatatattg atttttttag ttgtcgtgat ataatagttc cgtaaggaga
       61 tttgatatgg taagtttaga agaagacaag gtgactgttc aacctgatat taaagtgatt
      121 aaacgagatg gtcgccttgt taattttgat agtacaaaaa tctatagtgc tttattaaaa
      181 gcaagcatga aagtaactcg gatgtcgcca cttgttgagg ctaaattaga ggctatttct
      241 gatcgcatta tagcagaaat tattgagcgt tttccaacta atatcaaaat ttatgaaatc
      301 caaaatattg tagagcataa gcttcttgca gctaatgaat atgctattgc aaaagaatac
      361 attaattatc gtactcagcg tgactttgca cgttcacaag caacagatat caatttttct
      421 attgataaat taattaataa agatcaaaca gttgttaatg aaaatgctaa caaagatagc
      481 gatgttttta atactcaacg agatttaact gctggaatcg tagggaaatc gattggttta
      541 aaaatgttac cttcgcatgt tgctaatgct catcaaaaag gagatatcca ttaccatgat
      601 ttggattaca gtccttatac accgatgacg aactgctgtt taattgactt taagggcatg
      661 ttagccaatg gctttaaaat tggtaatgct gaagtggaaa gtcccaagtc tattcaaact
      721 gcaacagctc agatctcaca gattattgcg aatgtagcat caagtcagta cggcggatgc
      781 acagctgatc gcattgacga gtttttagcc ccatatgcgg agcttaactt taaaaaacat
      841 atggctgatg ctaagaaatg gatcgttgag actaagagag aaagctatgc ttttgaaaag
      901 actcaaaaag atatttatga tgcgatgcag tctttggagt atgaaattaa tacgctcttt
      961 acgtctaatg gtcaaacacc atttacttct ttaggatttg gtttggggac gtcttggttt
     1021 gaacgtgaga ttcaaaaagc tattttgacc attcggatta atggtcttgg tagtgaacat
     1081 cgcacggcta ttttccctaa attaattttc acggttaaac gtggcttgaa tttagaacca
     1141 gattcaccaa actatgatat taagactttg gctttagaat gtgcgactaa gcggatgtac
     1201 ccggatatgt tatcttatga taaaattatt gatttgacag gatctttcaa atctccaatg
     1261 ggatgccgct ctttccttca aggctggaaa gatgaaaatg ggcaagatgt gacctcaggc
     1321 cgtatgaatc ttggggttgt caccctcaat ttacctcgca ttgccatgga atcaaatggc
     1381 gatatggata agttttggga gctgtttaat gagaggatgc taattagtaa ggatgcttta
     1441 atttatcgtg tcgaacgtgt cacagaagca aaaccagcaa atgcccctat tctttatcaa
     1501 tatggtgctt ttggaaagcg tttggagaag acagggaatg taaatgatct ctttaagaat
     1561 cgtcgtgcaa cagtctctct tggctatatt ggtctttatg aagtggcgtc tgttttttat
     1621 ggtggtcaat gggaaggtaa tccagatgct aaagctttta ccttgtcaat tgtcaaggca
     1681 atgaaacagg cctgtgagga ttggtcagat ggatatggtt atcatttctc tgtttattcg
     1741 actccatcag aaagtttgac agatcgcttt tgtcgtttag atacggaaaa atttggcatt
     1801 gtgacagata ttacggataa agaatactat acaaattctt ttcactatga tgtgcgtaag
     1861 agtccgacac cttttgaaaa attagatttt gaaaaagatt atccagaagc aggtgcttca
     1921 ggtggtttta tccactactg tgagtatcct gttttgcaac aaaatccaaa ggccttggaa
     1981 gcggtttggg actatgctta tgatcgtgtg gggtatttgg gaaccaatac gcctattgat
     2041 aaatgctata attgccaatt tgaaggcgat tttaccccaa cagaacgtgg ttttacttgc
     2101 ccaaactgtg gcaataatga ccctaaaaca gttgatgtgg tcaaacgtac atgtggttac
     2161 ttggggaatc ctcaggcccg cccaatggtt aacggtcgcc ataaggaaat ctctgcgcgt
     2221 gtaaaacata tgaatggttc tactataaaa tacccaggcc tgtaaactca agctggggga
     2281 gagccctttt gatggaaata tgatatgctt aaaaggttgc ttcatgcaag caatctttta
     2341 ttgtttaatg gtttcttagc aattgtgaga taagaataac taatccaaaa cgactaatta
     2401 gctgttagag caatcggact ataagaagaa agagataagg aagaaataat gggaaaatat
     2461 caattagact ataaaggaat gcagcaggta gaacgctttc atgagaagca ttctaaaaaa
     2521 aagacggata aaaaatcccg ccttcaagaa cttaaggctc ggtttttaga gaagtcaaaa
     2581 aaacaggata actaaagtat ccagtaaaga aagaaggttg agtatgctta atattgggat
     2641 tgtgggacta ggtgctattt cacaaaaagc ctatttacca tatatgagac agctgagtga
     2701 cattacttgg catctatcaa cgcggaatgc agcagtacgt cagcaggtcg gtcagttatt
     2761 tggtcacgct attctttaca gtgatgtcaa ggagttgtca aaaacaaact tagatgggat
     2821 ctttattcat gcggccacat cggcccacgc agagttggct agtttatttt taaatcaggg
     2881 gatcccagtc tttatggata aacctatagc tgacaattac ctgatgacaa agaacctcta
     2941 tgacttagcc aaagaaaatc agacttttct gatggcagga tttaacaggc gttttacacc
     3001 tcgtgtcaag aagttgtcaa gtttgtcaac taagcgtaaa gtggctgttg aaaaaaatga
     3061 cttgaaccgg ccaggagata tggcttttaa gctctttgat ttctttatcc atcctttaga
     3121 tacagcctta ttcttgacag aagggacctt actaaaaggg cattttcaat atcatctgga
     3181 agcaggctta ctgagtcaag ttatggtaac cctgatgacc gaaagcatga cgactacagc
     3241 ttctatgaat ttacaatcag ggagtcgccg tgaggtaatg gaagttcaac gggctgaaga
     3301 aacttatcat ttagagaact tagacgagtt atctatttat aaaggtactg aaaaaagggt
     3361 gcttggcttt gcttcttggg acactacctt gcacaaaaga ggttttgaaa caatgatcga
     3421 tgccttttta gaagctatta gcacaggcgt caatcctgtc agccctgagt ctagtctttt
     3481 aagccactgg atttgtcaac agattgctga ctctcagctt tcttatggag agttaacggt
     3541 agagttgcct aaggattagg aattgatatg gaaataagac gaccgacttt aaaagataaa
     3601 gatgctgtat tgtcaatgat taacgaattt ttagagcaga aaagtgctac agatgggtta
     3661 tggcatttta acgtcaatga cttcaactat gaaacatggt tggaagattc cttgcgacag
     3721 gaaatgggtt tgtccagtca aggagtgcct gctattcaat acgtcgcatt tgatgagaga
     3781 agtcaggcta tcgggttctt aaatcttcgt ttacggttaa atgagagatt acttgaaaaa
     3841 ggtgggcata ttggttactc tgtccgtccc agccagcgtg ggaaaggtta tgccaaggag
     3901 atgcttaaac aagctgtcag ctgtgcgatt tccaagaata tcacgacaat tttggtaact
     3961 tgtgatgaga ctaatgttgc aagccgagca gttattgtgg ctaacggcgg tgtcttggaa
     4021 gatagtcgag gtggaacgga acgttattgg attgaagaat aaagaggcga taagtgatgg
     4081 cagaaaagtg ttggaataat cctaagccaa agcaatggca ggcagaggag ttaagtcaag
     4141 gccggattat tgactataag gcttttaact ttgttgatgg tgaaggggtt cgtaattccc
     4201 tttatgtgtc tggttgttta ttccattgca aggggtgtta taatgctgcg acttggtctt
     4261 ttaaggcggg aatgccttat acgcaagaac ttgaagagca gataatgacg gatttggcac
     4321 agccttatgt tcagggactg acacttttag gtggagaacc ctttttgaat acgggtatct
     4381 taattcctct gattaagcgt attcggtgtg aattgccaga aaaagatatt tggtcttgga
     4441 cgggttatac ttgggaagaa atgatgcttg aaacaccaga caaactggaa atgctgtcct
     4501 taattgatat tctggtagat ggtcgttttg atatcacaaa aaagaacctc atgttgcaat
     4561 ttagaggctc ttctaaccag cgaattatcg atgttcaaaa gtctttggct gctaaagaag
     4621 tgattatctg ggataagtta aacgatgggg accaaacctt tgaacaaatt agtcgggagg
     4681 atttgcttta agaagctgac ttatccacat gatttttttg aaaaggtcat gtgaaaacag
     4741 gtcccttaag ggtgaattgg ggagaaagta attgttattc cctggttttc cccacattat
     4801 cctcatagtt atccacaggt tgtgtataag ttgtagaatt gtgaataaag actaaaaaag
     4861 aggcctaggc ctcttttttg atatagacaa attcatgatc tgttgatagg tctggatggt
     4921 agtaaaaacc agcaaagaca aggcttttaa ggctgtcaac agtttggcaa ttattttcca
     4981 tacaggcagt aagaaatgca cctcgtgcct tttttgaaat ggtggaatgg gttttgaatt
     5041 ggccctcttt ttctgccata aacttgggac ctatccacaa ttgtttacag tctttggaaa
     5101 agacatcgtc aaattcactg gataaaaggg aaatcacttg gggatgctct ttggcaaact
     5161 gattataaca gggccgccag taagacttga ggctttgtcc ttcaattttt attctggtgt
     5221 gaaaatcatg gcggtgttct gcgataggat gattagcggg aatgataccg taaaaggatg
     5281 aggtgatgta aacctgctgg gtgaggtaag cctgttcttg ggtagtgagc ttatcgcgct
     5341 tgatatggcg gtacataagg ccattgaaca gttggtaggc agggtaagcc aagctttgtt
     5401 gagaggtcat gtcttgccag cgttgctgtt cttttttagc tgcctcttct ttgatacgat
     5461 aggctttggc caagtcctct gtggtcatag ctgccattgc ctttaaaatg gcctggcttg
     5521 gttggggcag taggtgtgga taagattctt taggaatcac catttctttt gcggttggaa
     5581 ttaaaaaggt tagcatagtg ttattgtagt aaagttactt gctttctgtc aagaaaacta
     5641 gtcttgtcca aggatgacgt tcaaacggct accttcctcg tcttctgtcg tgaaagaaga
     5701 agaatcttct tgtaaaatag ctaggccata tcatctggct tttttgagag tggctgaaaa
     5761 aagcaggggc gtctcaatgt gaatggttaa gaaggctaga cctggagtgc cttcttggtg
     5821 ttttttgagg taaggggctg accagtgatt gaatgcaagg tgatgatggt agttccctga
     5881 agcaatccag ctagcacttg gaatggtcat tttatcgcct aaatcaaaaa ccttttgata
     5941 gagaagtgaa gaatccagag catttttgac actcagatgg acatgactaa tgcgtgtgtc
     6001 ttgagctagt aaaaagtgct tggggatatc tgtcaactgc tccaatatgc ttttggtatc
     6061 cgtgggctca gtcacaccaa taatttgacc attgtcgcgg atatcccaat gctcaacagc
     6121 tttgtcatga tagatttcaa tcccattacc ctcaggatcg ctgaggtaga tagcttcgct
     6181 atagccgtga tcagctgctc cttctaaaga gatacctctg gttaagaaat gaagtcccaa
     6241 gctatgacga tcaggaacta aaaaggctgt gtgataaaga ccataagcct tatttgctgg
     6301 taaaggtgtt tgtcgtaact ctaagataac ggttttaccg tctgtagtca attgacgaga
     6361 tgtcgtgtct tgtgagagaa cctgtaaacc gataatactt gtgtaaaagg ttgtcatctt
     6421 tgctagatcg gtaacattga gggaaacctt tcctagagag atggttgagt tgtaagggta
     6481 tgtcatccta ttgtctccaa aagtgattaa gcctatgata acttattagt ttcaaaaaga
     6541 aaagtaggca caaataagtg gtataggcac taaaaagtaa ctcatttgtc agccctatct
     6601 tgtttctgtt ctctctaata gtctataatg ggttataagt tacaaaaaga tactaagagg
     6661 agctgctgcc tatgctgaaa aaaagaccgg cttgtcccgt agaaacaacc ttatcggtaa
     6721 tcgggaataa atgggaatta ttaatattaa gggacttgct aaaggggacc ttgcgttttg
     6781 ggcagttgaa gtcctcaata ggttcggtca gtcaaaaggt acttactgcc caattaaggg
     6841 ctatggaagc agacggattg gttcatcggg aattgtatgc agaagtgcca cctcgggtgg
     6901 agtattcctt aacagaaact gggttgagct tagctcctgt tattgaagcg atgtcggatt
     6961 gggaccaaac ttaccaagaa aaacatgccg atttatttga agaaggctag aggaaagagt
     7021 tgtccgagca tactgagact tagagaagct gagagttttt ttaaaatgat attatcaaat
     7081 tctttacagt ttgataaaat tacttttttt ctacaaaaag acttgcgctc atctcacgaa
     7141 tatgataata taatatcaag tctgtttgag aaaacagaag ctcaggtcaa gtaagtgcac
     7201 acttacaata acacagttga taatggggaa actcagagtc aagaaaaaga aaagcaagcc
     7261 ttgcattttg caaggctttt ttctgtcttt attttggtct gcgccgagca aaatctaaaa
     7321 atttaaattt ttccagttta tggcgactct cggtgaattg gaattggtta ttattggaaa
     7381 ggtaaacttt ggatttgacg gaaacaacat gttggtctga tccaaggtca agtaaaattt
     7441 tatcacgatc ggttatttgg tcaatggtaa cctctttgag ggcaaagtca atggcgagat
     7501 ggagttcctt ttccaaatat tgataaatag aatgctctgc gatttccctt gtcattattg
     7561 gcaccaaagt tttactaagg taatcaatgt ctaagacgga agccacaccc tcaacaacgc
     7621 gttggcgcgt aacacgccag accaagctgt ttttgctaaa accggtcaat tttgaaagtg
     7681 tctcatccac aatcagttta tcaatggcaa tgacgttggt cttagaatct agatttgagt
     7741 aagagacgag ttcctgataa ctagttagtt ctgagatagg gaacatgatc tgatgatgtt
     7801 taatcacttg ggtgccacgt ccttgttttt tgaggataag acctgccttt gtgagaagtg
     7861 agagagcctt gcggacggta tctcggctag cttggtattg ttgactcaat tctatttcag
     7921 taggtaaaaa atccccttct ttgtaaaagt ctttgtttat ttttgtttcg aggtctttat
     7981 aaatacgttc atactttgtc atagggtaaa ttacctttcc acttgtctat ttttttacag
     8041 acaacacctt ttattttacc aaaataatgg cttttttggc acaaacaact tgcagacaaa
     8101 tttgcaatcg attacaataa aggtgtcaat caggttctat ctaagaattt gaatatttca
     8161 ttttgacgtt gcttgttaaa agcaactaga acaaaggact aggggatgtc acatgggaaa
     8221 atttgaacag gatgctaaaa gtcttctaac tgctattggc ggtaaagaaa acatcaaggc
     8281 tgtcacacac tgtgcaacgc gtatgcgttt tgttttgaat gataataata aggcaaatgt
     8341 caaagagatt gaaaaaatct ctgtagttaa agggacattt accaatgctg ggcagtttca
     8401 ggtaatcatt ggtaatgatg ttccagtttt ttataatgac tttacagctg tttctggtat
     8461 tgaaggggtg tctaaagaag ctgccaaatc agcagctaaa agtaatcaaa atgccttaca
     8521 acgggtgatg accatgttgg ctgagatttt cacacctatt attccggcga ttattgttgg
     8581 ggggcttatt ttaggtttcc gtaatatttt ggagagtgtg ccttttgaat ttcttgggca
     8641 gcaggtcgaa aaagggaaat tagtttttga tgcagctggg gatcctgttt ggaatacgat
     8701 tgtgagggta tctcctttct ggtcaggggt taaccatttc ttgtggttac caggggaagc
     8761 tattttccac ttcttaccag ttgggattac ttggtctgtg acgcgtaaga tgggaaccac
     8821 tcaaatttta gggattgtcc ttggtatctg tttggtgtca ccacaattat tgaatgccta
     8881 tgcggtagca ggaacgcctg ctgctgagat tgccaaaaac tgggtttggg attttggttt
     8941 ctttaccatt aatcgtattg ggtatcaggc acaggttatt ccagcccttt tagctggtct
     9001 gtcccttgct tatcttgaaa ttttctggcg taaacggatt ccagaagtgg tttcaatgat
     9061 ttttgtgcca ttcctttctt tgattccagc tttgatttta gcgcatacgg tattggggcc
     9121 aatcggttgg attattggta aagggatttc ctttgttgtg ttagctggat tgactggtcc
     9181 tgttaaatgg ctattcggtg ctatctttgg tgccttgtat gctccgctag ttattactgg
     9241 tttacatcac atgacaaatg ccattgatac ccaattaatt gctgatactg caactcgtac
     9301 aactggtttg tggccaatga ttgctctttc aaatatcgct caagggtcag ccgtttttgc
     9361 ttactattta atgaatcgtc atgaagaacg tgaggctgaa atatcgcttc ctgcagcaat
     9421 ttctgcttac cttggggtaa ctgagcctgc cttatttggg gttaatgtta aatacgttta
     9481 tccctttgta gccggaatga ttggctcagg tattgcgggt ctcttatcaa caacctttaa
     9541 tgttcaggca aattctattg gtgttggtgg cttaccaggt ttcatggcta tcaatgtgaa
     9601 gtacatgatt ccattcttca tctgtatggc agtagccatt gtggtaccga tgtttttaac
     9661 cttctttttc cgtaaatcac atatcatgac taagacagaa gatgaggcta aactacctga
     9721 gacacccgtt tcggaggctc ctgcagcaac tgctccacat aagactatgc aagggacagt
     9781 tatcacttta acaagccctt taacgggtga agttaaagcg ttgtctgaag ctgttgatcc
     9841 tgtctttgca cagggagtta tgggtcaagg tgctcttctt caaccgacag aaggggtgtt
     9901 agtagcgcct tgtgatgctg aagtatcggt cttgttccca actaaacacg ctatttgttt
     9961 ggtgacgact gaaggtttgg aattattgat gcatattggc atggatacgg ttaacttagc
    10021 tggtcaagga tttgaagctt tggtgaagca aggtgatcag gttaaggctg gacaaacatt
    10081 gattcaattt gatatagcag ctatttctga agctggatac gccactgaaa cgcctcttgt
    10141 ggtgactaat caagatgctt ttacggtaac tgttgaaggt aacttaccgc atcagattaa
    10201 ggctaatgat aagttagtag tagcgatgaa aaagtaggtt tactagggag aaaacgttca
    10261 ggaactgaga tcagttcctg aaactatttt agggaggaaa actcatgaca attgataaaa
    10321 agaaagtcgt ctatcaaatt tacccaaaat cctataagga cactactgga aatggtgtgg
    10381 gagacttgcg agggatcatt gataaattgc cttacttaca agaactagga atagatatga
    10441 tttggttgaa ccctttctac cctagtccac aacgagacaa tggctatgat gtttcagatt
    10501 atacgacggt caaccctgat tttgggacga tggctgattt tgaagatttg gtaaaagctg
    10561 ctaaggagca tcagattgag ttaatgttgg atatggtttt gaatcactgt tccacagacc
    10621 acgagtggtt ccaaaaagct ttagcaggag acccttatta tcaggatttc tttatcttga
    10681 gagatcagcc gactgattgg gtttccaaat ttggtgggaa tgcttgggcg ccttttggag
    10741 atacaggcaa gtactactta cacttgtttg atgtgacaca ggctgacttg aattggcgga
    10801 acccacatgt tcgtgaggaa ttggctaaag tggttaattt ttggcgagat aaaggagtga
    10861 aggggttccg gtttgatgtg attaatctga ttgggaaaga tgaagagctg gtggattgtc
    10921 cggtcaatga tggtaagcca gcctatacgg atcgtcctat tactcacact tatcttcatg
    10981 atctcaatca agccagtttt ggtcaggatg attcgtttat gacagtaggg gaaatgtctg
    11041 ccacgactat tgacaactgt cttttataca cggctcccga acgtgaggag ctatccatgg
    11101 cttttaattt ccaccatcta aaagttgatt atgagaacgg tcagaaatgg actattatgg
    11161 cttttgattt tgcagcgctg cgagacttat tccatgcttg gggtgaaggc atgagtcaag
    11221 gcaatgggtg gaatgccttg ttctacaata accatgatca accacgtgcc ctgaatcgtt
    11281 ttgttgatgt aacacatttc cgaaacgaag gtgcgacgat gttagcggct tccatccatt
    11341 tgtcacgagg aacgccttac atttatatgg gtgaggagat tggcatgctt gatccagact
    11401 ttgatagtat ggatgattat gtggatgtgg aaagtctcaa tgcttactca agcttattag
    11461 tctcaggtaa aagtgcgaaa gaagcctttg ccattatcaa ggctaagtca agggacaatg
    11521 ccagaacacc aatgcaatgg gatgctagtg aacatgctgg ctttacgact ggtaagcctt
    11581 ggttagaggt tagcaaatct tatcgagaca tcaatgtcga aacagaaaaa gagggacgta
    11641 tttttccttt ctaccaacgc ttgattgctt tgcggaagga actgcctatt attgctgaag
    11701 gggactatcg ggctgctttt aaagatagtc aggctgtcta tgcctttgaa cgccatttag
    11761 gtgaccagtg tttgcttgtt ctcaatcatt tctatgctga tgaggtcgaa ctggaattac
    11821 ctccacgtta tcaacatgga caggtcttaa tcagcaacta tgagaaagtc tctatttgtg
    11881 aaaaagtgat actgaaacct tatcagacac ttgctatctt agctgataac taactaaaag
    11941 cctgaggtta ggcctcaggc ttttggtgat tgtcacgaaa aaagaagttt tctcatatat
    12001 ttatttttaa actcttattt tttctaaata aaaacaattg agataagtta ttttaaccaa
    12061 attttggtac aatggaaaag attataagta gagtttaaga taaagattct acaaatttaa
    12121 aatgatataa ggagatacga atgacaactt atcaagatga tttttaccag gctgtgaatg
    12181 ggaagtgggc tgaaacagca gttattcccg atgacaagcc tcgaacaggt ggattttcag
    12241 acttagcaga tgaaattgag gcattaatgc tagacactac agatgcttgg ttagcagggg
    12301 atcatgtccc tgatgatgca attttagcaa actttgttaa atttcaccgt ttggtggctg
    12361 actacgctaa acgtgatgaa gttggagtaa gtccaattct ccctttaatt gaagaatatc
    12421 agtccttaaa atctttttca gaatttgttg ctaatatagc gaaatatgaa ttggcaggtt
    12481 taccaaatga atttcccttt agtgtggcac cagattttat gaatgctcag ctgaatgtgc
    12541 tttgggcaga agcaccaagc attcttttgc cagatacaac atactacgaa gaaggcaacg
    12601 aaaaagctga ggaattgcgt ggtatttggc gtcagtcgca agaaaagctt ttaccacaat
    12661 ttggtttttc aacagaagaa atcaaggatc ttttggataa ggtaattgaa ctagataagc
    12721 aattggccaa gtatgtcttg tctcgtgagg aaggctcaga atatgccaaa ttataccatc
    12781 cttatgtgtg ggctgatttt aaaaaactag ctccagagct tcctttggat agcatttttg
    12841 aaaagatttt aggccaagta cctgacaagg ttattgttcc tgaggaacgt ttttggacag
    12901 aatttgcagc cacttattac tctgaagcca attgggattt actcaaggct aatttgattg
    12961 tggacgcggc taatgcttac aatgcttact tgacggatga tattcgtgtg gaatcaggcg
    13021 cttattcgcg cgctctttca ggaacacccc aagctatgga caaacaaaaa gcagcctttt
    13081 atttggcgca aggaccgttt agccaagcgc ttggcttgtg gtatactggt caaaaattct
    13141 ccccagaagc taaggctgat gtggaaagca aggtagcacg catgattgag gtttataagt
    13201 ctcgtctaga aacggctgat tggttagcac cagccacacg tgaaaaagcc attactaaat
    13261 taaatgttat cacgccacat attggttacc cagaaaaatt accagaaacc tatgctaaaa
    13321 aagtgattga tgagagcttg tcattggttg aaaatgcaca gaacttggct aaaatcacga
    13381 ttgctcacac ttggagtaaa tggaacaagc cagttgaccg tagtgagtgg catatgccag
    13441 cccacttagt caatgcttat tatgatcctc agcaaaatca gattgtcttt cctgcggcaa
    13501 tcttacaaga gccattttat tctttagatc agagttcttc agccaattat ggaggaattg
    13561 gtgcagtcat tgcgcatgag atttcacatg cctttgacac caatggtgct tcttttgatg
    13621 aacatggtag cctaaatgat tggtggacac aagaagacta tgctgccttt aaagagcgta
    13681 cagataagat tgtggcgcag tttgacggct tagagtcaca tggtgctaag gtcaatggta
    13741 aattgacagt ttcagaaaac gtcgctgatc taggtggggt tgcttgtgct ttagaagcgg
    13801 cacaatcaga agaagacttc tcagcccgtg acttctttat taactttgcg acgatttggc
    13861 gtatgaaggc ccgtgaggag tacatgcaaa tgctagctag cattgatgtt catgccccgg
    13921 gtgaactgcg aacaaatgtc accttaacca actttgatgc tttccatgag acctttgata
    13981 ttaaagaagg cgatgctatg tggagagctc cgaaagaccg tgtgattatt tggtaattag
    14041 gtagttgata aaccttcaaa tgcctgtgat tataaaaatg actgattctg tgaaacacag
    14101 gatcagtttt tttcttcctt attggtgtcc gttttgacac aacaaaaaga caaccctcag
    14161 gaaagaaggt tgcctttttt agacttatca gaaataagtg attagttgtt aagggcagca
    14221 gccatagtag ctgcaacttc tgattcaaag tcgttagctt ttttctcgat accttcacca
    14281 acttcaaaac gtgcaaaagc aatcgcttta gcgtttactg agtcaaggta ggcttcaaca
    14341 gttttgctgt cgtccatgat gtaaacttgt gcaagaagag tgtaagcttg gtcaacttta
    14401 gtgttgtcaa gcatgaagcg gtccatttta cctgggatga ttttgtccca gatcttttct
    14461 ggtttgcctt cagcagcaag ttcagctttg atgtctgctt cagcagcagt aatcacgtcg
    14521 tctgataatt gagcttttga accgtatttc aagaatggaa gtgctggttt gtcaaccatt
    14581 gcacgcgatt cgttgtcaag ttcgatagcg tggttcaatt gagcaagttc atctttgatg
    14641 aattgtgcat caagctcagt gtatgaaaga acagttggtt tcattgcagc gatatgcatt
    14701 gatacttgtt tagcaagagc atcgtcgccg ccttcgacaa ctgagataac cccgatacgg
    14761 ccaccattat gttggtaagc accgaagtgt tgctcatctg ttttttcaat caaagcaaaa
    14821 cgacggaatg agattttttc tccgatggta gcagttgcat taacgtaagc ttcagcaaga
    14881 gtttcacctg aaggcataac aagtgcaagt gcttcatcgt tgttagctgg tttgccttca
    14941 gcgataactt tagcagttgc gtttaccaat tcaacgaatt gagcattttt agcaacaaag
    15001 tctgtttcag cgttaacttc aacaacagca gcaacgttac catgaacgta aacgcctgtt
    15061 aaaccttcag cggcaacacg gtcagctttt ttagccgctt tagccattcc tttttcacga
    15121 agaagttcaa cggctttgtc catgtcacca tctgtttcaa caagtgcttt tttagcgtcc
    15181 ataacgccag caccagattt ttcacgtaat tcttttacaa gcttagctgt aatttctgcc
    15241 atgtgtagta tcctccaaat gatatttgtt ttggtttcaa aaaacgagac agagcggttt
    15301 gctttctgcc ccgtttggtt ttcgattgtt aaagacaaac gaagtttgcc ataagagtta
    15361 ggaactctgt gctaattaag cgttgtcgcc ttcgacaact tcaacgattt cttcgatcga
    15421 atctgcttga gtgtcagctt caaaagcaac gtctgcatct tcaccttgac ggccttcgat
    15481 aatagcgtca gctaatttag cagtgattaa tttaacggcg cggatagcgt catcgttagc
    15541 tggaatgatg atatcgatat catctggatc agcgtttgta tcaaccatcg ctacgactgg
    15601 gataccaagt tttttagctt ctttaacagc aatttgttct ttatgtgggt caacaacata
    15661 catcacgtct gggatacgag gcatatcttc gataccgccc aagaattttt caagacgagc
    15721 gcgttgtttg ttaagaagtg caacttcttt cttaggaaga acgtcaaagg ttccttcttc
    15781 ttccatacgt ttgatttctt tcaaacgagc gatacgtttt tggatagttc cccagttggt
    15841 aagcgttcca cccaaccaac ggtggttgat gaagtattgg cctgcacgcg ttgcttcgtc
    15901 agcaactgct tcagcagctt gttttttagt accaacaaac aagatgacag catcgtttgc
    15961 tgcagcatca cgaacaaatt cgtaagcttg gtcagctaat tttacagttt gttgaagatc
    16021 gataacgtgg ataccgttac gctctgtgaa gatgtattta gccatcttag ggttccagcg
    16081 acgagtttgg tgaccaaagt gaacaccagc ctcaagaagt tgtttcattg aaattactgc
    16141 catgagtagt ttctccttta taaaatgttt tttcctcttc cagacttcga cttgcaggct
    16201 aacctagcgg caacaagccc acaattggtc tagaatgagt atttgccgtc tacacgaccc
    16261 tactatatta tcaaaaaaag aggttaaaag caaggagaaa tgctatggat tcccttattt
    16321 ttgagatgac tagcttcttt gtttattggt aagtgttaag acaagctttt gctcattttg
    16381 ggtgacactt gccaaatcgt tttatttaag atatagtgaa tttgactaat ttttggaggc
    16441 gttttcatga cgaagggtat tcgatttcaa ttgcttggca gtccccacat ttttcttgat
    16501 aacaaagaac aattttttgc ctttgccaaa gcaaatgctc tcctttatta cttagtggtt
    16561 aatggatctg ttagccgcga ggtggcggcg agtctacttt gggaaaataa gaacacgcaa
    16621 acagcgaaaa agaatttacg aaacgctatc taccaggtca ataaagtctt gcaagctgat
    16681 gtgattattt gtcccaatcg caacctgctg gttttgaata aaacacttga tatcaaaaca
    16741 gatattaacc tttttctggc tgatccgcta gctcacttag aactctacca aggggagttt
    16801 ttacagggct tttaccttaa aagtggtgag gaatttgatc tttgggtttc aaaaatgcgt
    16861 atgcagtatg aacaggtata cctaaaagcg tgttaccaaa aaattgaaga aaagctctct
    16921 ttagatgcca ttgaagatgt cgaggaacat ttaaaacagc ttattgaacg tgatgaattt
    16981 gaagagaaaa attatcagct cttgatgagg ctttatcagc aggggaattg tcctggtaag
    17041 gtcatcgaaa cttattatca gttggctaat gtgttggata aggagctagg gattcagcca
    17101 agtttgcaaa gccaacagat ttatcaggaa gtggtcgcta aagatcgtaa tgaacgcaaa
    17161 atcaagcatt ttttgcgcaa cagtaatcat tttttggggc ggattgatga gattaagcag
    17221 ctggaaaact attttgccaa ctgtttggcc tgccaagagg taggagctct gctcttaatt
    17281 ggtgatacag ggattgggaa acgaacccta gccagacagg tcttagccaa tcaaacccaa
    17341 acgtttcaaa ttgtcacagc taaatgtttt cgtgaagagg ctatggattc cttattgcct
    17401 tggcgtaata tcttagatgg cctgggagat ttggtgattc aaaaccgctt attgaccacc
    17461 aaagcttgga aggctgctct taaacgctgt tttcctgtgg caaccatttt tcaagaagat
    17521 aacaaccaac cctttatcaa ggaccacacc agcttgttgg tttcctttat tgttgatatt
    17581 ttacaacatt tggcagaaat caaggcgctg gtaatcctta ttgaggattg tcattggatg
    17641 gatgaggata gtttgacctt gttgcaacgg gtcatgaatc aattggtcca ctatccgatt
    17701 gcttttgtct tgaccaagca tctaggaacg actcctgagc tcggtctttg tttgaatgct
    17761 ttgatgagtc aggggcgttt agaaagcatt tgtttggagc cttttaatcg gcaagaaagt
    17821 ttggcttata ttaacagtca attgggtagt cagccagtga cggaagaaga aatggagcac
    17881 ctctatcaag ccagccaagg caaccctttc tttttgtcag aatacactca tgccctactg
    17941 cgtcacgaaa aatttgtgcc tttaacgcct gccattaagg ctaagttggg tcttaaacta
    18001 gctaatctaa gtagtcgtga tgacgcattg ttaaactatt tgtcttgttg tcggaggcct
    18061 atccctctaa ataccctcgc tcaattgatg ttactgcctt tagaagaagt gatcgagatg
    18121 gtggataatc tggggcacta ctatattttg gtagaagaaa gcgtaggaga ggaggtgttg
    18181 atttcatttc gtcagcgaat tattcaactc tatagttatg accgtttatc tttgtcaaaa
    18241 aggcgtttat tgcacggaca aattgccaaa cggttagaag atttactgcc tattttgaca
    18301 ccaagccctc acttattaga tgacattgct taccattatc aggaatcacg gcaggtgatt
    18361 aaggctttgg aatacaacct taactatcta gatgctacct tgccatttca gcatgagctg
    18421 tttcccattt attcaaagag tattggctcc ttggaaaagt cagatcgtga ccatcaacgc
    18481 ttaatggaag agcagtttga taagattcgg caaagcattg cggatttgga actgacctat
    18541 gataataacc gtgatttcca gcagttgctt attcgttttt cttaccttga aggccgttat
    18601 gatattcgaa cagggaggta tcaagaaggg attaaacata ttcaaaaggt catcgctttg
    18661 gcaaccgagc taaaacagcc ctccttcttg ctagaaggct atcgccagtt aattcattac
    18721 tgtatccaag tggaaaacaa gccagaaatg cgttattaca ctagtctttc attggaggca
    18781 gcggttgctg ctaatcattt tgaggccatt gcgattagtt tgcggttaaa cggtctttat
    18841 catttgatta ttggggagtt aaatgaggca gagcgtctgc ttcaacagtc gattgacttc
    18901 tttaaggtga cgccaggctt gcaagctaac tatgcgattc agattgctgc tgccttagat
    18961 tatttggggg agatcgccca gattcgctat cagtttgaaa aggccgttgc ttaccaaaaa
    19021 caagccattg ccttgaccga aaacaagcca gctgagttat cagtgagtat tttttatatt
    19081 ggattaggga tttcctactt ttatttagct gattttgaac aagctgagca aatcttgagt
    19141 ttggctaagg aagctttggt caatcatagt tatccttgga aagaaacgca acttgaaatt
    19201 tacctggcca tgattcaatg gaaaaagggt aattatcagc cagccttgac tctacttgat
    19261 tacagagaaa ccttgatgtc taggtatcgc aatccgcgag ataagggctt ggttttctat
    19321 ttgatggctg tggtcaagta ccaattgata cgtcaaggag ctaccctaag tcagcaggaa
    19381 aaggaaatgg cagaccacct gttgtcagaa tcctttgagt attattatga gattgctagt
    19441 actaacctca acccttaccg agattgtcat ttggtgagtg agttgaacga tttgcgtcaa
    19501 caattatctg ctaaaagcta aggaaaaccc tctgattcac atcagagggt tttagttatg
    19561 ttgtgccata atctgtacga ggtaaaagat aaaggtggct gctaaattgg cggtgtggtt
    19621 atcaatatcg tgtggtggtg aaacctcaac cacatcaaag ccaactaatt ttccgctagc
    19681 tgcaatgtgt tgcaaaacta agacggcaag atttgggtca acaccgaggg actggatagc
    19741 actaacccca ggtgcagcac caactgagaa acaatccatg tcgatagtca ggtagaccct
    19801 ttcttgccct tctaagaaac gatcaatggc tcggcagacc ttttgatggc ccatttggta
    19861 gatgtcttga cctgttaaaa attgaatgcc cttggatttt gccacaaagt caaataggaa
    19921 aaggttgtta ttatgttctt gaatgcctaa aacgaagtat ttaaagaggc gtttgtcagc
    19981 aaccgcatcg tcaaacattt gtcgaaagcc tgtccctgaa ttagggcctg tttggtcgta
    20041 aggccttaaa tcaaagtggg cgtccatatt gatgacagct aggtcatcac ttgaggataa
    20101 gctttggcgt aggcctaagt agtggccgta ggcggtttca tggccacctc ctaagacgat
    20161 gggtttgaga ttgaggtcac acattcgttt gatggctttg cttaaactgt tttgcagttg
    20221 ttccaaagag cgattggggc cgtcaatgtt acccacatcg taaaccataa cttggtttcc
    20281 taaatgccaa gggaatttgg ctagctgggt tcgtatggca gctggacttt cgaccgctcc
    20341 gacacgacca ttattgatat aaacgccctt atcactttta aagccaatca aggcaaaatg
    20401 tgtcccttca aatggggtta gtgagctgtc gttaaggtca aggaaagtca tgaccatgcc
    20461 ccatttggca gtatataagt catcgtcgat cccactatga taatagctag tggtggatgg
    20521 gtagtaatct tctagcatgc gcgtcatctc ctttaggatt atgatagcaa aataaaactt
    20581 acagtcaact ctctataggc tgtcatcact ggtttttagg aatagcagag caataaaatc
    20641 gtctgctttt ggtcccagtt agttagtcta aagaggccat gacctctgcc acagcccctc
    20701 tcagttaaag aagtacctta ttcactaaaa ttggatactc aactcaactg cctgctcaac
    20761 cgcttcgagg aagcttgggt cttcaatgac tgttgatgcc ttgtttagtt catcgtagat
    20821 ttcgatgtgt ttatcgtgtt cgataaagtt gacttccttg cggaagagat cgtaggcaac
    20881 cttagtccct ttacctagtt catgattttc agatttgaga tcaagagctt ggcaggctgc
    20941 catgatttca gtagccacga tgcggcgaga atttttaagg atttcaaagg ccttgcgagc
    21001 agcggtggtg cccatgctga caaagtcttc ttggttctca caagatggga tagaatccac
    21061 actggctgga tgtgccaaaa ccttgttttc agaagcaaga gaggcacagg cgtactgggt
    21121 aatcataaag cctgaattga gacctgggta tttgacaaga aaggatggca atttgctgag
    21181 ctggctattg accaaacgtt ccacgcggcg ttcagaaaca tttccaattt cagaaatggc
    21241 gatgcctaag aagtcaaatg gctgtgccat tggttctccg tggaagttac ctcctgaaat
    21301 aacgtggcca tctttacaga taattggatt atctgtgaca gaattgattt caatgtcaac
    21361 tttggatttg acataggcga tactgtcttt gctagcccca tgaatttgag gcatgcagcg
    21421 caaggtgtaa gggtcttgca cacgagattg agtagctacg gttgtgtttt ggctgccttc
    21481 aatgaggttt cggatattgc gggctgttgc caactggccg ctttgtgggc gaatggtgtg
    21541 aaggttttct tcaaatggac tagtaatccc gttatgcact tcaagggata gggcaccagc
    21601 taggtctgac agtttcaata attgaatggc gtcataggtt gcaagggcac cgattgcagt
    21661 gagaacagtt gttccgttga taagggcgag tccttcttta gcggctaaag aaatcttgtc
    21721 aataccagct ttatcaagag cttcttgacc agaaagaagc tctcctttgt agtaagcttt
    21781 ccctagacca agcataggaa gcaccatgtg agcaagcggc gccaaatctc ctgaagcccc
    21841 tagagaccct ttttcaggaa tgtaaggatg aaccccttta ttgagcagtt ccaacagctt
    21901 ttcaatggtg gatagacgaa taccagaata ccctttgacg agagagttga tacggatcag
    21961 catgatagcg cgcacggcat cttctggcaa tgggtcacca aatccactag catgggtacg
    22021 aatcaaattt tcttggagtt gaactgtatc ttctggtgag atgctcacgt tacaaaggga
    22081 tccaaaaccg gtggtcaccc cgtaaaccac ccgtttttca ctgacaatgt catcaacaat
    22141 tttacgagag gcattcacag cttcaatagc actatcatca atgcgacagg ctatgccttg
    22201 gcgagcaatc gcaatcacgt cttcgatagt aaggctttcg ccatctaaat taatcactct
    22261 tgtcatagtt tttctcctag atttgttcct tagggggcat ttggtccatt tggtggttac
    22321 gtccgtgcat aaggtagtac acactacttg caatgatgac accaactgca ccataaacaa
    22381 agttcattgg gttgtcaccc caaatcataa ctaaactaac gataactgca agaattggga
    22441 taataggacc aaatggtact cggaagatca cattagcatc gggatcgtcc ttccgtaatt
    22501 tcataacggc tagtgctgtt ggaatgtatt ggaagaagcg gaaaataaca ctgagttttg
    22561 ctaagttttc aaatgagcca gtgagtagga gtacaatagc aattgctcct gaaaccaaga
    22621 tagcaacgag tggtgcgcca ttttgatttt gcttggcaat agcagctggt aagagccctt
    22681 catcagcaat ggcagcaccg taacgtggca ccatgatgga ttctcctatg ttaagacctg
    22741 taatggaaat taaagcaccg attgagacca tccaagcgcc agcagggcct atcattttaa
    22801 caaaggcatc ttgaacaggt gcattggtca tcatgatttg agagccaagc atggcaatgg
    22861 ttccaccgat aatcagcata tataagacag agacgatact gattgatcct aaaagagcac
    22921 gcggtacatt tttttcaggg tcacgcattt ccccagcgac aatagagagg gtttcaaaac
    22981 cgataaagcc atagaagata taaacagcag tattggagat agcccctaag aggttagtcc
    23041 ctggttccaa ctgaacaaat ggggtaaagt taggaagccc gtttttgatg aagaaaaggg
    23101 tacaggcaca aaaggcaacg attggaatta acttagcaat ggtggctgtg atggtgacaa
    23161 ttttagaggt tttcaaacca gcgatattca tcaaagatag taagatgatt aacccaatgc
    23221 taagtggaat gtgccaacct tcaaaggcag ggaaagtaat gataaacatt ctggcaaacc
    23281 cagctgccat ggctgcccaa gcgaagatgg taacggtcca accaaggaag ccaacgttaa
    23341 agccaataaa atcaccaaaa gctcgtttgg aatattggaa ggccccgccg tttttcccaa
    23401 aataaccaga gacttctgca aaacaaactg ccaacatgat ggtcaaaata gctgttccaa
    23461 acataacagc gatggatgca gggccaaggc ccttgtaaat ggccctaggt aagagaaaaa
    23521 taccagagcc gataacggca ttgatcccgt aaagagtggc accgctcaag ctaaactttg
    23581 cctgttctct ttcttgttcg ttcatgtgat ttgacgccat aataatgctc tcctttttgt
    23641 ttgcaaattg cgttcaaata taatggataa ggtcgctagg aaatgaggag atagtcggtt
    23701 taggagggtc cttcttgcac ctaacgatag tgatgagcta ttggttagcg atgagaaata
    23761 ggtagccttt tgtgatgatg tgtagtgatt tcattggaga agaataggca tgagtagtgt
    23821 gatgcctatc tttatcccac taagtcttat accataagcc tgcccaaaca gctactggag
    23881 tttgggcggg gcttgtttga ttgaggcaaa cttttagatc tgaaaggctg gcctcctgct
    23941 cgattaccaa caagtgattg gcttcaagct cttgattcgc ctgattggtc acagaataag
    24001 tcagtttgct tagggcataa agcagttgac agtccgcttt aggtgcctca aatagtgtgt
    24061 cagaccagac gggtaataat tgcccttggt attggtcatc atacattaaa ttcatgtctt
    24121 ggcagattcc acgactgacg attctggtat cgccttcaaa cgcataaggt tcaaaaggag
    24181 ttagggaaat ctctttagca tgactcaaat cactcaatgt gatgggcttg tctagggtca
    24241 ttagaatcct gtgatagcca tcaagtaagg taaaggttgt ctgtcgtagg gccacagttg
    24301 actttgagag gcgataagag aattctctct ttttgtaatc tccatcttcg ggataaagga
    24361 acaattggtt ggtttcaccg ccagaccagt cagtttggag gtaatcagag ggcgctttga
    24421 tagttacctt tgtcatttgt tctctccttg tttccaatta gaatagtcct gtgatgttgc
    24481 cctcctcgtc aatatcaatg gtttcgctgg caggtacttt tggaaggcca ggcatggtca
    24541 tgatagcacc cgtcagagca acgataaatc ctgctcctgc agacactttg aggttgctaa
    24601 tagtcactgt aaagtcagtt ggtgcgccaa gtgttttggc atcgtctgag aaggaatatt
    24661 gggttttggc catgcaaatt gggtagttgc caaagcctag gcgttctaag tcagccagtt
    24721 cacgtttggc tgctgggctc aaggtgatgc ctttaccacc gtaaactttg gtaacgattt
    24781 tagtcagttt ggtttcaatg ctatcgtctt cctcataaac aaagcggaat tgattgtctt
    24841 gctcggctag tgtcacgact ttttcagcca gttcacggcc accagctccg ccatttgccc
    24901 aaacatcaga aatcacaacg tcaacgccac gtttgttgca ggcatcatag acagcttgta
    24961 attcagcatc ggtatcaagc gggaatttat tgattgcaac gacaactgga aggccataga
    25021 cgtcttggat attagccaaa tgtttgtcaa ggttaggcaa accatccaca acggcttgaa
    25081 cattttcagt agccaagtca gttttttgaa cgccaccgtg cattttaagg gcacgaatag
    25141 tggctactag gacaacggct gctggacgaa ggcctgacat gcggcatttg atgtcaatga
    25201 atttttcagc tccaaggtca gcaccaaatc cagcttcggt aacggcataa tcaccatatt
    25261 tcaaggctag tttggtggcg aggacactat tacagccatg ggcgatatta gcaaatgggc
    25321 caccgtgaat gagggctggt gtgtgttcca gggtttgcac gagattagga tgaatcgcgt
    25381 ccttgagcag agcggctagg gcaccaccag ctttcaagtc tttagctgtt acaggttcgc
    25441 cttgatagtt gtaaccgatg atgatttttt caaggcgggc tttgaggtca gagatatttt
    25501 cggataggca caggattgcc atgatttcag aggcgacggt aatgtcgtaa ccatcctcac
    25561 gagggacccc gttgaccttg ccttgcaagc cgtcaacaat atgacgaagc tggcggtcat
    25621 tcatatccac cacacgtttc caagtaatac gtctggaatc aatgcctagg ctattgccat
    25681 ggtgaatgtg gttatcaatc aaggcagcta ataagttgtt ggcgacaccg atggcatgaa
    25741 agtcccctgt aaagtgaagg ttgatgtctt ccattgggac aacttgcgcg tggccaccgc
    25801 cagcagctcc tcccttaacc ccaaaaacag gaccaagaga aggttcacga agggcaataa
    25861 ctgctttttt tccaatggca gaaagagcat ctaccaatcc aacggaagtg gtggttttgc
    25921 cttcaccagc tggtgttggt gagatagcag tgacaagaat gagtttgcca tcaggtttgt
    25981 cttttaaggc gaccagttga cgggcattaa tttttgcctt gtacttacca tagagacaga
    26041 gcgcttcctt gtcgatccct aattgatcgg caaccttact gataggttcc atagagacag
    26101 agttggctat ttcaatatct gataaaacca tagttgctcc tttacttgtt aaacaatatc
    26161 gagaattttc gtgtagatgt catctgccaa gtgacagccc ttgtctaata aggcttgtcc
    26221 tttttggcga tagtcggtaa caaagtcttc atctttgatg ccagacagat taatcaaaac
    26281 attcaaccaa gcgccttgta aacctgcttt taagtttaaa gcagcaactc ctaagtcgct
    26341 agcagcattt gtattagact taccgacagc tgttgcagtg atctcaagtg cttcgaccat
    26401 gagggtcatc atctcaaaag gtgattgtgc agctgttttc aaggcctttt gcatggcagt
    26461 tcgtctagca gccttgtctt cgtcagtctc ttttggcata tcaaaaacgg cagaaaccag
    26521 gttgaaggct tcggtatctt tgtcaatggc tgcaagcaag ctagcttgta gagcggtgct
    26581 tttagcatgg acctctgtaa taatatcttg gtagtcagcg tattttttct taccgagggt
    26641 caattcacag accatcttag tcaaggaaat gccattagcg ccagagagag cagcagcaga
    26701 accaccacct ggatcagggg catctgagcc aaggacttta gcgaaatctg ttaagcttaa
    26761 atcaactaaa cccatagaga acctcctaac ctagcaaatg gttttcaaga acctgtttat
    26821 ggtagtcaaa gtcctcaact tgtaagtagt attcagccac atcgattaag gccttggctg
    26881 gcgctaagcc gatgacttca gaaccaataa cattcacacc gtagcgacgc gcttcaaatt
    26941 tgatggtttc aaaagtacgg taaagagaac atttttcaaa gttaaccatg ttcatggaga
    27001 cttgggcaat atggcggtct tctaacatga caccgatagc cttacaatat ttgtagccac
    27061 cacctgaacc gcggataatt ttcgcaattt tatgagcaat gtcgatattg tcagtatcca
    27121 aattaacatt gaaggcaacc aatggcatgc gagcaccaac ggcagttacc ccagcagttg
    27181 ggtgaatctt acgatctccg tagtcaggtg cccaatcctc ttccaacagt ttttctggca
    27241 tgccttcgaa ttgtccttta cggactttgg ccaggttttg acgttctggg cgtgtggcag
    27301 aatcttcata aaggaagatt gggattccaa gttcacggtt aatccgttct gcgacttgtt
    27361 tggaaatctc aacacattct tgtgtagtga tgtctttgat cggaacgaat gggcagacat
    27421 cggttgctcc catacgaggg tgttcgccat gatgtttggt catatcaatg ttttcagaag
    27481 cgtatttcac cagttggaaa gctgcttctt gaatagactg gtcatctcca accaaggtaa
    27541 acacgctccg gttgtggctc gcatcagaag agtagtcgag aagggtcact ccaggaatac
    27601 ttttggccgt cgctaccaaa ccatcgataa cagcctgatt ttggccttct gagaagttag
    27661 gaatacattc aacaattttt gccatgttaa tctcctttta tgtcattgag ttactcatta
    27721 agggaatagg ttacctactt tagataacgc ttacattttt agctagcagt tattagtgaa
    27781 ataattgatc taccgctgaa atcacaaggt catcgtcagc caaatatggg atagtgatat
    27841 gatcagtacc tgcatggaga cggttgtatt caatagcggt ttcaatagcg tgctcattgc
    27901 gagcccaatt gcgacgagca acaccaccca tggtgtccca agcaatggca gatttaataa
    27961 tctcatcaat gcgttcgctg ccatctaaaa caagaccgaa accaccattg atggctttac
    28021 caataccagt tccgccaccg ttatgaagag ccactaaact cataccacgc gcagcgttac
    28081 cagcgtaaca ttgaaccgcc atatcacagg tcacattgga accgtcttta atgtttgagg
    28141 tttcacgaaa tggtgagtcg gtaccagaca cgtcatggtg gtcacgacca atcatgacag
    28201 gaccaatctt gcctttgcgg accaattcat taaatttaag ggcgatagtc acacgaccga
    28261 tacagtcttg gtaaaggatc cgggcttggg taccaactac gagttgattt ttctcagcat
    28321 cacgaatcca gttgtagtta tcacggtctt gataacggcg gtcaggatcg atagcttcca
    28381 tggcagcttt gtcagtggcc accaagtctt cgtgtttacc actcaagcag acccaacgga
    28441 aaggtccata gccatagtca aaaagcatag gtcccatgat gtcttcaaca taagaaggcc
    28501 agataaagcc gtctttgtcg ttacgaccat tcttagaaat ttctgtaata ccagagtcgt
    28561 aaacagactt cataaaggca ttaccgtagt caaagaaata ggtgccattt tcagttaacg
    28621 tcttaatagc ttcaaaatgc cgtgccaagg tatcatcaac catttggtgg aaggtatcct
    28681 tgtcttctgc caagagccgg gttctttcgt caaagctaat gccagctggg cagtagccac
    28741 cgtcataaat gttgtgacaa gaggtttggt cagacaaaag atcaacatga atctgcttgt
    28801 cattgacgta ctctaaaaga tcaacgatat taccatggta agcaatcgaa gtggactcct
    28861 tagcatctat ggctttttgg gctaattgca gagcttcttc agggctctca gcaatttggc
    28921 taacccaacc ttgcgaatga cgggtttcaa tccgagattg gtcaacttca gcaataatgg
    28981 caactgcttt agcaatttca gcagccttac cttgtgcccc actcatgcct cctaaaccag
    29041 atgagataaa gagtttaccg gtcaaatcac catcgtcagc cactccaagt ttgagacgac
    29101 cagcatttaa gagagtatta aatgttccat gaacgattcc ttgtgggcca atatacatcc
    29161 aaccacctgc ggtcatttga ccatagttgg tgacacccat ctcttcagcg atttcccagt
    29221 ctttcatgtt gtcgtattca cccaccaaaa ggccattagt aataatcaca cgaggcgctt
    29281 caggtttgga tttgaaaaga ccaactggat gaccagactc aaccaccaag gtttgttcat
    29341 cagtcatgac ttctaggtat tttttaatca aacaatattg catccagttg gcacaaacag
    29401 aaccagtttc tccatatgtg actagttcgt aagggtacaa ggcaatttca aaactcaagt
    29461 tattgtcaat catgacctgc atggccttgg cagctgtaca atttcccttg tactcgtcaa
    29521 tgggtttgcc gtaaatgcgg tcttttggtc taaagcggta gccataaata cggccacggg
    29581 ttttgagttc ttccaaaaat tctggaatga cttcttcgtg gaacttcgta ggtacatagc
    29641 gtagggcatt tttcaaggcg atttcggtct gagcctgggt caatcgaaaa cctctgtctg
    29701 gtgctcttcg gataccttct tcaaacacag tcttttccgg taaaacatcg tctaacttaa
    29761 cagtcatagc ggcagctatg tcagtttcac tgtaaaatga catagaaaac tcctcctcaa
    29821 ctccttttta ttgtcgagat tagtctaact tagtctagtc tataaaaagt caaccacgct
    29881 ttgaaatgcg aaaaaagtct aaaaaaaaat caaaacctca ttttttgact aaaatttgac
    29941 gcttaatcag tacacttgag gtatgacaga taaagtcagt cagaggttaa ttgttgttac
    30001 caaagttgca aagatcgcaa ctattgagtg ggtgaggggg gactagtaat ggctattaat
    30061 cagttgacaa acgatgttaa aaaaggaggt ttttatggtt gctgatgtct tattaacaca
    30121 ttttaatcaa ctattttgtc taaatgatcc aggtcatcct ttaactggtc aagaaatgaa
    30181 aaaagccact atcgttgaag atggctatat agccatcaaa gacagcctta ttgtggctct
    30241 tggttctggt gaaccagatg ctgagcttgt cggtccacag actattatgc gttcttataa
    30301 aggtaaaatt gcaacgcctg gtattattga ctgtcatacc catttggtct atggtggcag
    30361 tcgtgaacat gaatttgcca agaaattagc aggggtgtct tacttggata ttttagctca
    30421 aggtggtggt attttaagca cggtgagggc cactcggtca gccagctttg acaaccttta
    30481 tcaaaaatcc aagagattgc tagactacat gttgcttcat ggagtgacta ctgtcgaagc
    30541 gaaaagtggc tacggccttg attgggaaac agaaaaacgc caactggatg ttgttgctgc
    30601 tttagaaaaa gatcatccca ttgatttggt gtctacgttt atggcagccc atgcgattcc
    30661 tgaagaatac aaagggaatc ctaaggctta tctcgatgtg attatcaagg atatgcttcc
    30721 tgttgttaaa gaggaaaatc tggcagaatt ttgtgatatt ttctgtgaaa aaaatgtttt
    30781 tacagctgat gagtctcgct atctgttaag caaagccaaa gaaatgggct tcaagcttcg
    30841 tattcacgca gatgaaattg cgtctatagg cggagttgat gtcgcagcag agctcagcgc
    30901 agttagtgca gagcatttga tgatgataac agatgatggt atcgctaagt tgattggtgc
    30961 tggtgttatt ggtaatttgc ttccagccac cacctttagt ttaatggaag acacttatgc
    31021 ccccgctcgc aaaatgatcg atgccggcat ggctatcact ctctcaacag atagtaatcc
    31081 aggcagttgc ccgactgcta acatgcagtt tgtgatgcaa ttaggttgtt ttatgttacg
    31141 tttaacaccg attgaagtct taaatgctgt taccattaac gcggcctact ctgtcaatcg
    31201 tcaggagaga gttggtagtt tgacagttgg taaagaagca gatattgcca tttttgatgc
    31261 cccaaatatt gattacccat tttatttctt tgccaccaac ttgattcacc aagtctataa
    31321 aaaaggacaa ctcacagttg accgaggtcg tattctttaa aaaatttaaa aaagaagctt
    31381 tgagcgttag ctcaaaactt cttttaaata tgctgtttta ttgacgaatc aagtagtcaa
    31441 aggcaccgat ggcagcagta gcaccagacc ccatggaaat aatgatttgt ttataagctg
    31501 aatcagtaca gtccccagca gcaaagatac caggaatatt ggttgaccca tgtttatcca
    31561 caatgatttc tccgcggtcg gttaggttga caccactatc tttgagccaa gctgtgtttg
    31621 gaacaagacc aatttgaaca aagacccctt caaggtcaag gtgtttgtct tcaccgctat
    31681 cacgctcagt gtagttaaga cctgtaacat ggtcctctcc aacaatgtcc ttagtagaaa
    31741 catttttgat gatggtcata ttgttagtct tagctgcgcg gtcttgaagc actttgtcag
    31801 ctttaagttc aggcaagaat tctaaaacgt aaacgtgttt agcaagacca gccaagtcaa
    31861 gggccgcttc taatccagag ttcccaccac cgataacagc aacatctttg ccttcaaaga
    31921 gaggaccgtc acagtgaggg cagtaagtca cccctttatt acggaattca tcttcaccag
    31981 gaacattaat gttacgccat ttggccccaa gagctaagat agcagttttg gcttgtagaa
    32041 ccgcaccatt agctagagta acctcaatgt tttctttttt ctcaatagaa gtagctagtt
    32101 gtgctttgat aatatcaaca tcataagact tagtatgggc ctccacttcg gccattaatt
    32161 ttggtccttc agtgtaaagg gtaccaatca tgttttcgat gccgaccgtt tccatgacct
    32221 gaccaccaaa ggtttcagca agtaaacctg tttttagtcc tttacgagct gcatagatag
    32281 cagcgctatt gcccgcaggg ccaccaccaa taacaaggac atcatagagg cctttatcag
    32341 caaaggcttc ctcagaaagt gggccagcga tttgttctaa gagttgctca atggtagcgc
    32401 gaccagaagt gaattcttcc ccgtctaaaa agacagtagg gacagacata atgccttttg
    32461 ctttcacttc atcttggaac atgccgcctt ctaccatagt gtgtgaaatc ttgtcgttca
    32521 agacggacat aatgtttaaa gcttgaacaa catctgggca gttatggcag gttaagctga
    32581 cataagtttc aaaatgcaac ggacggtcaa tggccttgat gcgatcaata acatcttgat
    32641 ccacttttgg agcacgtcca gatacttgca agagagcaag aataaaggac gtcaattcgt
    32701 ggcctaaagg aagaccagca aagacaaccc cactaccgtg accttttttc gcaaccttaa
    32761 agcttggttg tcggtctaaa gtgatgtttt caatagaaat acgctcagac atagcagcta
    32821 tttcttccac aaagtctttg actttttgag actgctcatt gtcaccaagg gaaacttgca
    32881 aaacaagatc agcttctaac agggtgaggt attgggcaag ttgttcttta atatcaggac
    32941 ttaatgccat agctttcctc ctaaaattta ttaaatttta ccaactaaat caagacttgg
    33001 tgtcaaagtt tcagcgcctt ctttccattt agctggacaa acttcacctg gatgtttacg
    33061 gacgtattgg gcagcgtgaa ttttatcaat caaggtgcta gcgtcacgtc caataccatc
    33121 agcattaatt tccatcattt ggataatacc atctggatca acgataaatg ttccacgttg
    33181 agcaagtccg tcttcgccaa gcacttcaaa ggcttgtgaa ataaggtgtg aagggtcacc
    33241 aatcataggg tatgtgatag tgccaaccac atctgaatca tcatgccaag ctttatgaac
    33301 aaaatgagta tcagtagaga cagaataaac ttctacacca agagatttca gtgtttcgta
    33361 ttgcttttga aggtcaccga gttcagttgg gcaaacaaat gaaaagtctg ctgggtagaa
    33421 acaaaaaact gcccattttc ctttaacgtc ttcatttgta acagtgatga attttccatc
    33481 gtgataagct tgagctgaaa attcagcaat ttcttttcca attagagaca tatagttgtc
    33541 ctcctttttt atagttttat acttgtatta taaaagaaca acaagattat gtctactcag
    33601 aaacccttga ttttactggg aaatcagcga ttgttttctg acaattcagt tgtgaaacaa
    33661 atcacattaa ttaaaatgat tctaaataag ctgaagatgc tgttaaatca aaggttaatt
    33721 taattataat atacagaaaa tataattatt attaaagcgt ttacaaaaaa cttttttgat
    33781 tccgttaagg caacttaata ttatggttac ctaatcacta atattgtaac ctattttggc
    33841 atgagatcag ggaatggctg ttatattaat tggatatgtc ttgtgcaaat agtgcttgac
    33901 ggatttgtgg aattagggta aaataatctt tgttgcggcg gtatagccaa gtggtaaggc
    33961 acggctctgc aaaagcttga tcgtcggttc aaatccgtct accgcctttt atatacaaaa
    34021 caagccttga ttaacttcgt gaaaacgttg ttaaatcaag gtttttattt tttgcgacag
    34081 gataaaaaaa gagaaaaaaa gaaataaagt cggtcaaatt tcccgaagat gtcttttata
    34141 gcctgattat taggtatgaa aaatactatg gccaaaattg gtaaacgatt gcaaataaag
    34201 gtcagagttc ttaccccctt aaacgatatg cgttaaaagt tagttgaact gccgtgcacc
    34261 taacgataga cacggtgatg tgagagcgag tagagttgaa tctctacttg tttatcttac
    34321 catagtcagc tttataaagt aaccacccat tacaatgcaa ctatattctt aatgttttta
    34381 agcatgttat tgacaagtgt tataaaatgt gttactctta gtgagtaaat tagtttttga
    34441 gttagtattt gtgtttcaac atctttattt ctctctattt ttacaaatga tttttagaaa
    34501 ggaagtggat gtatatggca caaggtacag taaaatggtt taacgctgaa aaaggttttg
    34561 gttttatttc aactgaaaac ggtcaagatg tctttgcaca tttttcagcc atccaaacta
    34621 atggttttaa aacgttagaa gaaggacaaa aagtggcatt tgacgtcgaa gaaggtcaac
    34681 gtggacctca agcagtcaat atcactaaat tggcctaatc tggttaaagt aactaaagag
    34741 aagcagatgt tggaaacagc aactgctttt ttgatttcta ccagttacct ttaatgatta
    34801 agagttgcgt ttaaggtctt gtatttgtta taataatagt cagttttagt caaaatgtat
    34861 tgacgtggtg ctactaagtt tttagagtag cccgcaagca aaggagtgac agcatgccaa
    34921 ccaaaaatac ttcagacagc attgaagagt atattaaaga attattagct aagtcaggaa
    34981 tagcagaaat taagcggtcc atgctagcag attcgtttca agttgtccca agtcagatca
    35041 actatgtcat taaaacccgt tttacggaga gtcgaggcta tgaagtagag agtaaacgag
    35101 gtggtggagg ctatattcgt attgccaagg ttcatttttc agacaaacac catctgattg
    35161 gcaatttaat ggcaactatt gaagactgta tcagtgagca agttttcacg gactcgatac
    35221 aattgctttt tgacgaacac ttattgacag agcgtgaggg aaatatcatt ttggctgtgg
    35281 cgtctgatga tgttttagga actgatggtt caactattcg tgcacgaatg ctctatcgct
    35341 tattgcaacg aattgacaga aagggaagca attaatgatt atgtattcaa cgaagatgca
    35401 agacattttt agacaggcgc agttccaagc tgctcgcttt gatagccact acctggaaac
    35461 ttggcatgtt ttgttagcta tggtagctgt agacaattct ttagcaaata tgattttaag
    35521 tgaatatgat gcccaagtcg ccatagaaga atatgaagct gcagctattt tagccatggg
    35581 caaaacccct aaggaacagt tgtctcgtgt agacttcaga cctcaatcta aaactttgac
    35641 taacttgtta gcttttgcgc aggctattag ccaaatcact agggatcaag aagtcggctc
    35701 tgagcatgtc ttatttgcta ttttattgaa tccagatatt atggcgagtc gcttgttaga
    35761 aatagcaggc tatcagataa aagatagcgg caatgggcag ccgcgattag ctgacttacg
    35821 aaaagcaata gaacgtcatg caggttacag taaggaaatg atcaaggcta ttcacgaact
    35881 acgtaagcct aaaaaaacga aaacacaagg gaccttttca gatatgatga agccaccaag
    35941 tacagctggt gagttgagtg atttcacaag agatttgact gaaatggcaa gacaaggttt
    36001 gttagaatcg gtgattggac gtgaccaaga agtatctcgt atgattcagg tattaagtcg
    36061 taaaacgaaa aacaatcctg tcttggtagg tgatgcaggt gttggtaaaa cggcgcttgc
    36121 ttatggcctt gctcaacgga ttgcaaatgg cgctattcct tatgaactta aggagatgcg
    36181 tgtcctagaa ttagacatga tgagtgtggt agcaggaacc cgttttcgtg gggattttga
    36241 agagcgcatg aatcaaatca ttgatgatat tgaagctgat ggtcagatta ttctttttgt
    36301 tgatgaacta catactatta tgggttctgg cagtggtatt gacagtacac ttgatgcggc
    36361 taacatttta aaaccagcat tatcgcgcgg cactcttcat atggttggag caacaactca
    36421 agaagaatat caaaaacata ttgaaaaaga tgcagctctt tcgcgtcgtt ttgctaaaat
    36481 attaattgaa gaacctaata cagaagatgc ttatcagatt ttgatgggcc taaaattatc
    36541 ttatgagacc taccataatg tctcgatatc aaatgaagca gttaaaacag ctgtaaaaat
    36601 ggcacaccgt tatttaacca gtaaaaatct ccctgattca gctatcgatt tattagatga
    36661 agctagtgct gctgtgcaaa acatggtgaa aaaattagca cctgagactt taacaccaat
    36721 agaccaagct cttatcaatg gtgatatgaa aaaagtatct cgcctcttag ctaaagaagc
    36781 aaaaggtcag atgagaaaac caacaccagt gacagaagat gatattttgg caaccttgag
    36841 taagttatcg ggaattccac ttgaaaaact gacgcaagct gatagtaaaa aatacctcaa
    36901 tttagaaaaa gaactgcata agcgtgtgat tggtcaggat gctgctgtta cggctatttc
    36961 aagagccatt cgtcgcaatc agtcaggtat tcgaacagga aaacgtccta ttggatcatt
    37021 tatgtttctt ggcccaacag gagtaggtaa gacagaacta gcaaaggccc ttgcagaagt
    37081 tctctttgat gatgaagcag cgcttattcg ttttgatatg tctgagtaca tggaaaaatt
    37141 cgcagcgtct aggcttaatg gagcacctcc tggttatgtc ggctatgatg aaggaggtga
    37201 actgacacag aaagttagaa ataaacctta ttcagtcttg ctttttgatg aagtggaaaa
    37261 agcacatcct gatattttta acgttctcct tcaagtatta gatgatggta tattgactga
    37321 tagtcgtggg cgtaaggtcg atttttcaaa tactattatt atcatgacca gcaatcttgg
    37381 cgcaacagcc ctgcgcgatg ataaaacggt cggttttggg gtcaaagaca ttcaccaaga
    37441 ccatcaagct atggagaaac gtattttaga agaattaaga aaaacttacc gcccagaatt
    37501 tatcaatcgt attgatgaaa aagtggtctt tcatagtctg acccaagata acatgcgcga
    37561 tgtggttaaa atcatggtac ggcccctgat tactacattg gcagaaaaag gtattaccct
    37621 taaaattcag cctttggcct tgaaacattt gtccgaggtc ggctatgatg agcatatggg
    37681 ggcaagacca ttacgtcgaa cgctgcaaac tgagatagaa gataagctat cagagcttat
    37741 cctttctcga gaattgacaa gtgggcatac gctaaaaatt ggattatcac atggcaaatt
    37801 aacgtttcac atagcttaat ttccatataa aaggtggatc atccttgatg tcaccaaaaa
    37861 aagaggttat tcttacaaaa ataacttctt ttttcttgac tatttttgac caagtgataa
    37921 aatagaatat aaagttagca ctcgatgaac atgagtgcta actttactaa agattctttt
    37981 attggaggaa aatttcatgt taaaaccatt aggtgaccgt gtggtcgtaa gatttgatga
    38041 tgaaaaagag cagacagtcg gtggttttgt ccttgcaggc actcataaag agtcaacacg
    38101 aaaagcgaca gtcctcgctg tcagtgaaac gggtgttcgc actattacag gtgatagcgt
    38161 gctaccttca gtttcagtgg gtcaagaggt tttagttgaa aatgggcatg atttagaagt
    38221 aaccgtagat gatgagaaag tttctatcat ccgtgaatct gacatcattg ctattgttac
    38281 aaaataaaac gacaaatctc taaatagaaa gaaggattga atatggcaaa agatattaaa
    38341 ttttcagcag atgcgcgtgc tgccatggtg cgcggagttg atatgttagc agataccgtc
    38401 aaagtaacgc ttggtcctaa agggcgcaat gttgttcttg aaaaagcttt tggttctccc
    38461 ttaattacta atgacggggt aaccattgct aaagagatcg aattagaaga tcattttgaa
    38521 aacatgggag caaaattggt gtctgaagtg gcttctaaaa ccaatgatat tgctggtgat
    38581 gggacgacta ctgcaacagt tttgacacaa gccattgttc atgaaggact aaaaaatgtg
    38641 acagcaggtg ctaatccaat tggtatccgt cgaggcattg aaacagcaac agcaacagcc
    38701 gttgaagcct tgaaagccat tgctcaacct gtatctggca aggaagctat tgctcaggtc
    38761 gctgcagtat catcacgctc tgaaaaagtt ggagagtata tctcagaagc tatggagcgt
    38821 gtgggcaacg atggtgtgat taccatcgaa gaatctcgag gtatggaaac agaacttgaa
    38881 gtggttgaag gcatgcaatt tgaccgtggt tacctgtctc aatacatggt cacagacaat
    38941 gaaaaaatgg ttgcagacct tgaaaaccca tttatcttaa tcacggataa aaaagtgtca
    39001 aacatccaag acattttgcc actacttgag gaagttctta aaaccaaccg tccattactc
    39061 attattgcag atgatgtgga tggtgaagcc cttccaaccc ttgtcttgaa caagattcgt
    39121 ggtactttca atgtggttgc tgtcaaagcg ccaggatttg gtgatcgtcg taaagctatg
    39181 cttgaagaca ttgctatctt gacaggtggt acagtgatta cagaggatct aggacttgaa
    39241 ttaaaagatg ctacaatgac agcccttgga caggctgcta agattacagt tgataaagat
    39301 agcacagtaa ttgttgaagg ttcaggaagt tcagaagcta ttgctaaccg tattgcactg
    39361 attaaatcgc aattagagac aacaacttct gactttgacc gtgaaaaact acaagaacgt
    39421 ttggcgaaat tagctggtgg tgtagctgtt atcaaagtag gagctccaac agagacagct
    39481 ttaaaagaaa tgaaacttcg cattgaggac gctctaaatg ctacacgtgc agccgttgaa
    39541 gaaggtatcg ttgctggtgg tggaacagca cttattacgg ttattgaaaa agtagcagct
    39601 cttgaacttg agggcgatga tgctactgga cgtaacattg tgcttcgtgc tctagaagag
    39661 cctgtacgtc aaattgcttt aaatgctggg tacgaaggct ccgtagttat tgacaagttg
    39721 aaaaacagcc ctgcaggaac aggatttaat gctgcaacag gtgagtgggt tgatatgatt
    39781 aaaacaggaa tcattgaccc tgtcaaagta acacgatcag cgcttcaaaa tgcagcttct
    39841 gtagctagtc tgattttgac aacagaagca gttgttgcta ataagcctga accagctgcg
    39901 ccagcgccag caatgccagc aggtatggat ccaggaatga tgggcggctt ctaagctctc
    39961 tatagcatta aggattatga aaaagaagtt gagactaaag gtctgcttct tactatgaaa
    40021 aaagaacaaa gattggattt cgactcatga tatcccatct ttgttttttt agttgtgata
    40081 taaaatgcta atctttgtgg ttacagactt ttaatgtatt ggaaaatata ttgtttttct
    40141 ttgtcatgtg ctatcacgga gccaatgaaa agttattccc atatatgggt gttggaggtc
    40201 tgtgatgaat cattttggag agatttttaa aacctttaga gaatcaaagg gatcacgatt
    40261 aaaagatgtc gcaaaggctg gtatatcaac ctctcagctg tcgcgtttcg aaaaagggga
    40321 gacggactta accatatcga catttatgct gattttggat gaaagtaaca tgcccattga
    40381 tgagtttatg tatgctgtcc atgattttca tcgtgacgac ttaaatgaac tcttatcaaa
    40441 aagtgaggca tttcgtaaca attcaagata gtgatggctt gaagcagtta cttcatgctc
    40501 aactagcatc aactgataag aaggagatgt tcacggatat ctttttggca tatccagcat
    40561 gtctaagtct gctatggcat gccattttgg gagtccctca cgcgactctg tcagtagctc
    40621 tccttcaaag aaagattcac agggctcgac tttaccttct ggctggaccc atcctgggaa
    40681 gttatcgtgt tgtctatgga gtcataaaat catatcgtta tgtttgacac aaatattctc
    40741 ccagtcttta accgttgcta tcaccagatt caccaccttt tagtatagta taagaaaaaa
    40801 ggggtaaaag aggtttcttg ttgctttttt ggcttaaggt ttctttaagt cttatctagt
    40861 atactaatgt cattccttaa taggataagt atttggttgt agaagtatcg tcttaaacta
    40921 acgctatctc aaccgattcc catcttattc acccctccta taccgataag gtctcacaaa
    40981 gtgtgagacc ttattttttg acttcagctc tagttatgga gaaacttgtt gatacaagct
    41041 tttttgggtt atggtattat gatctattca agcctttctt gtttttgggt ttttctttaa
    41101 ccaacttggc ttcaatagct tttaagcgtt tgaaggtttt ttcagcgcga tctagtgaaa
    41161 tcttatcagc tttttcttga gcagctttgg ggtcactgac gactaagtca ctgatttctt
    41221 tggtgctttt ttcttgttcg gctatccagg ttttttccaa gcctttcatc tcatcaatca
    41281 ctttagttcc aaatggtttt ggatgagcag ccacgagatc attaatgtgt gacaccgtcc
    41341 aataccatga cttatcgtta tattgggtgc ttttttcttg ataggcttca taggttctac
    41401 tgatattgcc aaggtatggc aagtaagggg cattacgagg actgccaata gataaccaca
    41461 tgacaccacc acccaattca gcagggatgt cttttttcag ttggaaaata tgagcttcca
    41521 tgacattagg atttgagatg ggataagcat agcccttaac agcttttttg gattttggtt
    41581 tacccttacc gtctaatgcc atctgatcaa gtggtttaag atccagcccc tcaaaacgat
    41641 tacgttgcag tttcattgca tcttctagac taaaggtctt atcagtgctt tggagcaact
    41701 cataattgct gtctttataa gttacttttg aatcagggtc aagtgattta atccctgaga
    41761 aagagcgaga gcggttggca tcgtttagtg gcggattgta agatttggca atatgaaatt
    41821 taccatcaac ttcggtataa gatttggctt ttttagcgac tttttccaca tcttctgagg
    41881 caatcgtatt ttccttatcg ttgaaatcga catgtccgag atagaaggta tttggaaaaa
    41941 cagcgtattt atcatctggg aatttgatgg caacatattg gtgaccagag agaatttcca
    42001 tgtaccagat gccgtctttg tcagccaagg taacaatatt tccttcagca gctccttttt
    42061 cagtgactat tttagcaatc aatgcgacac cttcacgggc tgttttgaca cttggtaaaa
    42121 tgacactggc catagatgat tccgctaagc catttttgac gtaaggatca attttttgga
    42181 tggcatcatt agctgaagct gatactgttg cagacataga gacgccaaac tcattaaaac
    42241 cagcttcgtc ataaacgcct ttattaggtg ttacatcagg aatcgcacta tagcgataag
    42301 aatgctcagg caatgggtac tcaaaaccat tagaggggtc tttccatttt tcgccagcag
    42361 gattgtcctt tgctagacgt acaatgaaat ttttattatg gtgtggctca agatcttctg
    42421 ttcgaccata aagaagagaa ccgtctttgg ttaaatcttt gccaataata aaaccagtac
    42481 aagcataact aacactttga agcgaaaagg ctgtcagaat acttaaaact ccaagtgata
    42541 tttttttgtt tatcatgcga atcctcctag taaacgataa cgctttcaca gacattataa
    42601 aatgctcttt ttaaaaattc aagtctttta tgaagtgttt tgaaattgtt agctatctaa
    42661 atattatgag aattctctct tgaattttgg cttaacggcc cctaaaaacg tataggcagt
    42721 gaactatcta ggtttcttcg aaaatccatt agccatttat gatataatag taaagaaaaa
    42781 catagaaagt tggggtctgt tgatgaccaa accatttcat cataagaaac taaaacaaat
    42841 aactataata gctgcaacta gccttttttt attcctgatt ggtggtgcct tttactactc
    42901 taaaaatcat tgtattaatg cctatcttaa agctcgatca gcccaatcag gaccagtttt
    42961 tgagaatatc aaagcatatc tagtctggga tgatactaat gagcagatca caaatgacga
    43021 ggcgatgtat actaagttta gaaggtatag tcaaaaagaa ttgaggcaaa aaaagcagga
    43081 tttaaaagcc gctagtcaag atagtgccgt tcaagtaaag tctgttggtc gtcgtttttg
    43141 gatttttcct gattatcgga ttgccataaa acccatggat ttaacgataa aaacgaatgt
    43201 gcctcaagca gacgttcttt taaatcaaaa aaaagttgct gtttctgatt cagaacagtt
    43261 ctcagtcaag cttgatcggc taccaacggc agaatatacc gcaagtatca gaggcaaaca
    43321 caacgggcga aacattaaag tcaataaatc atatgatggt gataatcccg tgctagattt
    43381 gagtgtgtct tttagaactt ttttggtaac aagcaatgct aagcaaggag atctttactt
    43441 cgatgataac catattggca cattaaaaga tggtcaatta caagtagaag attaccctgt
    43501 tacagaaaac gcacaagctt atatgaaaac aacattccca gatggtgagt taagatcaca
    43561 aaaatatgct ctagctgatg ttgaagaagg agcaaccctg gagattttag ttacagatct
    43621 tttagaagag gacatagcag gggagctatt agtatcagct tttgatcagc tgatgcacta
    43681 cctcagtaca ggtcaagact catctaactt acgtagcgtc tttgaggcag ggtctagcaa
    43741 cgcattttat aggggattga aagagtccat aaaagcgaaa tttcagacag atacaagaaa
    43801 agccagccgt ctcaatattc catctatcct tttgacaaca atgactcaag tgggcaaaac
    43861 aacttacgtg cttgatttca cagctaccta cgaatttttg tatgacaact caacagatcc
    43921 tgagcagcat acctctggac atattaatca agacttgact ggaaaagtga ctgtaaaaaa
    43981 agttggacag cattacctta tcagccagtc cggctctaaa aatattactg ttgttaaaga
    44041 ggacaatcaa ctcaaagcgc catctgtctt tcctgagtct attttgggaa cgtggacagg
    44101 ccaagccaat ggtttaagca tccatatgtc tctagcatca gatggaacaa ttacgactaa
    44161 agttgaagat caaaaaggca accgttctaa agaaactcgg acagctaaaa ttagtaaagt
    44221 tgaagacaaa ggcaatggtt tttatcttta tacgccagat cctggaagtg acataagcgc
    44281 cttagctcca gaaggaggat tggggggtgc aaatgtcaaa tatgcttatg gtttcaaaat
    44341 atctggtaaa acagcctctc cagtggtgtg gcaggcagca ttaacacatg aatttgatta
    44401 taccaagcca ctttcgggag taactttgca aaagcaacca taaaggatgc aaagccacaa
    44461 agaacagtag aaacaatgtt attactcaaa tacagtgcta tgaaaacagc taaactcaaa
    44521 aggctaggag attatttcct agccttttga gcgtttaaga ctgcgtcaaa tgtagctgat
    44581 ttagcttgca cgcaaatgtc ttcttgggtt agagggtgtt tcaaacgtaa ttgataagca
    44641 tggagcatca gtcgagcaca atcttttttt ccattagaat acaaaggatc accaaataaa
    44701 acatgacctt gatgggctaa atggactcga atttgatgag tccgtccagt ttgtaattga
    44761 caattgagta agcttgccgt tttgtggaag tttttgacta gagttacctc agtaatagct
    44821 ttcttgccat ttatcggatc gacaacacgt tttctgcggt catgtcgatg acgaccaatt
    44881 ggatgatggt aagttaccct aggactgtct aaggatccgt gaactagtgc caaatattct
    44941 cgatggatgt ctcgtttttc taaaagacga ttaaggattg gtaagataaa aggtgtttta
    45001 gcaaataaaa tggccccact tgtttcctta tccagacgat gaaccacata gcaggtctgt
    45061 cccgtgtaag cagacacgtg gttgagcagc gccaactctg ttggatcatt gccgtgtgtt
    45121 ttcatccctt caggtttgtt aacaataatg atgtgctcat cttcataaag gcaggttact
    45181 ttttcggcct gccccatcac gataatcttt tcaggataat cttcgtgatc gaaaaaaagt
    45241 ttgacctgat caccgtattt aacacaactt tgccagttaa caggatgacc attaataaga
    45301 acatgttttt tagttctcag aaaatgccta attttacggg gaattaacaa ttgttcctct
    45361 aataatgctt tgactgtcaa ttcttcaaag ggatttttca gtgtgacagt aaaccccttt
    45421 gatggtttat ttgttgtctt cattgagcca gttatggtat ttttcaacaa ggttgatagc
    45481 catttgggtg gcaagagcag cagagaaagc ttcagtacct tcacgctcgt cattagctac
    45541 ctccaagcga gtctcatcgt aaatggcttt tagactttct gcggataagg tttctttaaa
    45601 agcttggaat ttattcattg gttaaacccg tccttctatg tttaatgtga gctcctgatt
    45661 aatgcaagga attcaatgct attatagcat gtttctaatc aaaacgtgcc actcaaaaag
    45721 gtaaaaacgt tattttgcta atccatctaa taagaaagtg tcttaaataa gacctcttga
    45781 tagagtttgt aacaaaaatg atatgaaact aggtgctaac gcagaaatag aaaccaacta
    45841 acgtgttgcg aagttggaac agtcataaaa aatagcaaga taaaacttat caacacacag
    45901 ttacctaaga cgatgagtca aacaggtatg ttctttcaaa acaaccccat gaggttgaac
    45961 gacgtatcgg aaaaatacgg attagtttgg ccttatgata ggtatttctc tatcgtaaaa
    46021 ga
//
LOCUS       DAUELZ010000004        43333 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.004, whole genome shotgun sequence.
ACCESSION   DAUELZ010000004 DAUELZ010000000
VERSION     DAUELZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43333
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.004"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(248..2053)
                     /gene="pepF"
                     /locus_tag="VHI09_000124"
     CDS             complement(248..2053)
                     /gene="pepF"
                     /locus_tag="VHI09_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ6120036.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEVEASKGFA
                     GHLLDSSANLLKVTKTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMMLHQEDYQAFLTETPELKVYNHFFDKLFQAREHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPIVRNDKGENVELTHGNFISLMESKDRSVRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
     gene            complement(2066..3028)
                     /locus_tag="VHI09_000125"
     CDS             complement(2066..3028)
                     /locus_tag="VHI09_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEQ6120037.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFRCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            complement(3323..4039)
                     /locus_tag="VHI09_000126"
     CDS             complement(3323..4039)
                     /locus_tag="VHI09_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ6120038.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPIHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTESERQLIKTYLD"
     gene            complement(4158..4862)
                     /gene="nagB"
                     /locus_tag="VHI09_000127"
     CDS             complement(4158..4862)
                     /gene="nagB"
                     /locus_tag="VHI09_000127"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEQ6120039.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYNQIIAEHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQKSTIEANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            5064..6092
                     /gene="queA"
                     /locus_tag="VHI09_000128"
     CDS             5064..6092
                     /gene="queA"
                     /locus_tag="VHI09_000128"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEQ6120040.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLKD
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            6099..7319
                     /locus_tag="VHI09_000129"
     CDS             6099..7319
                     /locus_tag="VHI09_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ6120041.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQDLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSALIQACYVNLEHIPYSRFNRKIHQLRYLISTQQMEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKATNSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(7433..8023)
                     /locus_tag="VHI09_000130"
     CDS             complement(7433..8023)
                     /locus_tag="VHI09_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEQ6120042.1"
                     /translation="MTERFDITETDYEYDQEHHAYVAQFNGAMSIPDMQEYSLVYSEN
                     TPAYALAERLGGMNKAYQLFDRYGKVSGAITTIDRNGNKITTAYYLQVLDYLWQHQDK
                     YKDILYYIGESFPDLYYKTYLPHVKVYQKPGYVREALNVGAIVCEESPYLIALYSSGL
                     GGATQASEEVNGLGYVQLVQLPYVINEWHRVNHNMI"
     gene            complement(8020..8268)
                     /locus_tag="VHI09_000131"
     CDS             complement(8020..8268)
                     /locus_tag="VHI09_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120043.1"
                     /translation="MDTDLVQMDALGLYYDDNHLSLVNTVKAYLKEFGIGESQIAFSY
                     KDLNSGRTVAMNDHQPMIAGSTYKLPLNMLVVDKANYP"
     gene            complement(8253..8470)
                     /locus_tag="VHI09_000132"
                     /pseudo
     CDS             complement(8253..8470)
                     /locus_tag="VHI09_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8637..9242)
                     /locus_tag="VHI09_000133"
     CDS             complement(8637..9242)
                     /locus_tag="VHI09_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEQ6120044.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            complement(9339..10379)
                     /locus_tag="VHI09_000134"
     CDS             complement(9339..10379)
                     /locus_tag="VHI09_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEQ6120045.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKTF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSHQ
                     LGLGFESGAFDQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKIDAARDLIHDLRLSGEDDIKLIAIMLGQFRLFLQLTILARDVKNEQQL
                     VISLSDILGRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            complement(10450..11031)
                     /locus_tag="VHI09_000135"
     CDS             complement(10450..11031)
                     /locus_tag="VHI09_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ6120046.1"
                     /translation="MRTHTDTDHMGDIEVVAKAIRIKEILTSQGSLSHPSFVRRLRRL
                     KCHVRVLAAGDQLPIMGSVLQVLYPWQLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEG
                     ENEIIKRYPQLRVDYLKAGHHGSNTSSSAAFLDHIQPKVAFISAGKNNRYQHPHRETL
                     ARLEDRQITYYRTDTQGAIRLTGWTSWHLETVR"
     gene            complement(11105..11680)
                     /locus_tag="VHI09_000136"
     CDS             complement(11105..11680)
                     /locus_tag="VHI09_000136"
                     /inference="COORDINATES: protein motif:HMM:NF015461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEQ6120047.1"
                     /translation="MNEFCYKLKGKILRTGQKWDLLSSSQADVPLFVAKYLNNEPPRY
                     VGKIPSDEYIIASSGPTINGRLRSGSVDEKIENIYQTLKKYSTKADIVVYRGVSMETL
                     EKMVESAQVEGCIDFKEKGFLHTSLVKGFEFRDPYKKLRIKIPKGTNAFYVGNLNNEE
                     THYYEVIIQKGAKLKVISIDDYINCELVGTD"
     gene            complement(11736..13301)
                     /locus_tag="VHI09_000137"
     CDS             complement(11736..13301)
                     /locus_tag="VHI09_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ6120048.1"
                     /translation="MKNVITIEANAPRNSELASISLPKKRRVAGYARVSTDHEDQTTS
                     YESQMRYYAEYISTRSDWEFVKMYSDEGISGTNTKLRVGFKSMIEDALAGKIDLIITK
                     SVSRFARNTVDSLTTVRKLKEVGVEIYFEKENIWTLDSKGELLITIMSSLAQEESRSI
                     SENVTWGLRKQFAEGKVHFPYTNVMGFEKGENGEIVVNQEEAKTVRYIFQQALLGKTP
                     YSIAKDLTQQGILSPSGKNHWSSVTIKRMLRNEKYKGDALLQKTYTIDFLSKKKNINK
                     GELPQYYVENNHEAIVDKETFDAVQASLDRKEKGKGSTTLFSSKLVCGDCGHYFGSKV
                     WHSTSKYRRVIYQCNEKYKGEKKCTTPHVTEDEIKDWFVTAINKVISNKDEIIQNLEM
                     LLDVGESSDLERRLVELETEVEVVTQMVSNLVKENAIMPQDQDTYQKKYSNLVERHDA
                     LVSEIESIEKQLLEAASHQKDLKEFLKTLNQQSELISEFDELLWETMVDEIKIFCDQT
                     VAIKFKNGIEVVE"
     gene            complement(13298..14536)
                     /locus_tag="VHI09_000138"
     CDS             complement(13298..14536)
                     /locus_tag="VHI09_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ6120049.1"
                     /translation="MRIVRRIQPMMTPQKPKLRVAAYARVSDSRLHHSLSTQVSYYSR
                     LIQSHPDWELVGVYYDEGISGKEQSNRQGLQDLLKACDDGKIDRILTKSIARFGRNTV
                     DLLSTVRVLRQKNIGITFEKEGIDSLSSEGELMLTLLASVAQEESHNISENLRWQIKK
                     KFEQGQPHTPQDIYGYRWNGENYVIEPDEAKVVRQVFQWFLDGDNAPMITKKLNEQGV
                     LTRLGNPFTVSSVREFFKQEAYFGRLVLQKTYREPFSRNPKRNNGQKNKYIVEDAHEA
                     IVSKSYFDVVLQEKERRYKVMYQESHMHKGTFRELIECSHCGKIMITRVDSKKVHRTI
                     RYCCRTRDRFGTSSCPSKTLSEKRILATFEDKIGFKPDKNWVTENIKKVIYDSSVGLI
                     TVFPIKGRKYELKVRKEHFL"
     gene            complement(14594..14755)
                     /locus_tag="VHI09_000139"
     CDS             complement(14594..14755)
                     /locus_tag="VHI09_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120050.1"
                     /translation="MTNDKLQDEMTYQLTMIQADRLLKSRIISEEVHQQFKEKMLEKY
                     QPFISRLST"
     gene            complement(14861..15637)
                     /locus_tag="VHI09_000140"
     CDS             complement(14861..15637)
                     /locus_tag="VHI09_000140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ6120051.1"
                     /translation="MTSKEKVVANALSKVNTKVTVPTNPYGGQCVALIDKIVQEETGK
                     NMSYTNAIDCLDKAKANGFQVTYDAVGVNPQAGDFYVIRVPSHSFGHIGVCLADSDGT
                     GLEGVEQNVDGYSDSNRNGVNDQLEVGGYTRRVSRKWYSDGRLVDSHSGGLVGYMVGW
                     FRLPYEVATSTKKVETSEDEDMKNFVVRSKSGKQGYVAVVNGAVFGIGHIDTVVQLQS
                     AGAAHLTLDDGDFNRFLESQKFDDEKLVASVQALEKAIKQ"
     gene            complement(15627..16043)
                     /locus_tag="VHI09_000141"
     CDS             complement(15627..16043)
                     /locus_tag="VHI09_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEQ6120052.1"
                     /translation="MKQEVLTNKLVFSGIGGVIGSIFGEIDGFLYGLLIFMLIDYVTG
                     IMAAIVEKNLSSSIGKKGIFKKAMIICLVAVAHMIDLHVLKQGGAVRTAVIFFYFSNE
                     GLSILENATRIGLPVPNRIKQVLRQLNEDKEDENDK"
     gene            complement(16054..17910)
                     /locus_tag="VHI09_000142"
     CDS             complement(16054..17910)
                     /locus_tag="VHI09_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120053.1"
                     /translation="MADYGSNNDRGYNLLLRVEETGTSTANNTSTVRVQLWLRNGQTT
                     FGMYNCSASVSIDGQTLSWSGRPDMYSMNSSLHLIDKTITIAHNSNGQKTIGFSATFS
                     GSGGWSPGTLNTGYQSLRLRDIPRSSTASVSGNMMGSPITISITRASGDFTHKVTWQF
                     GSLSGVVATGATTSCSWTPEVAKLAPQIPNATSGIGTILVQTIYGGKVIGESRIQVTL
                     QLPSSVVPSLSSISLSDLNTIAQNAITGNTFVSLESNPKVTFNGATGIYGSTIPASGY
                     RAEVFKFEGNSWIQLQNVATAQNGGVGGINHVGRAKISAFVTDSRGRQSPRKEVEISL
                     LEYFKPILSFTSVRVGTTMNQLNVIRKVKIAPLIVGNSQKNRATMTWDILDLSTGTKT
                     TNVGGAANWSTTTEHTKENFQATLNGTYDQTKSYTIIGHLRDLFYETDFEFTVGPEKV
                     VYGLSPTGMGIGKAWTRGVLDVDGSQPSYFDGDIIMRNKKLIDIFYPIGTIYESTSSE
                     SPSTFMGGKWERFGNGRVLVGVSETESEFNTVSKTGGSKTHTLTINEMPVHSHPQYVS
                     ANSGNQATRRDYSSDGSSSLYPQGNNTGNSGGGQAHNNLQPYITVYRWRRTA"
     gene            complement(17923..20811)
                     /locus_tag="VHI09_000143"
     CDS             complement(17923..20811)
                     /locus_tag="VHI09_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ6120054.1"
                     /translation="MLFLLDGQTKTPKWNGFPLHETLSAEVEETLNGDFTLQFDYPIT
                     DSGIYKELVTDKLILCPVPYVKKQLFRIKKPVEENDRVRVTCQHITDDIMRRSILPVS
                     NQNVTCQSALGQLISKAKRDIAPFSFDSDITKKRSFNTTETATLYQVLMDGKHSILGT
                     WEGELVRDNFLFTIKEHRGKNRGVVISTHHNLEKFDRTRSSQSIVTRIYASSIFKPEG
                     EKEEKTLSVMVESPLIHQYPYVHEAEYENNNLHTEEELRQWAMAKFSNDHLDQVSEVI
                     EIEAFELDGQEVQLGDEVTLKSRLHQVDVVKRAVGYRFNALTEQITSISFDDTVAFGG
                     QGVSGNPISSVANELLTLNDGKIQRQIERERANLSRVLDFNMSRVNKDVEDGIERAKA
                     EAIRSGNQAAVDYLSSEAAEIRFAAIQKAKIDELTVSNSAWMRRLVSHQVLTEYLNAI
                     EVNANKVVIPGSHKPVFSLDSNGNISIDTPLLKVRGEELATKSQLSKLSLTPGPKGDK
                     GVSISQIEEYYLASNQKTGVLTSTSGWSKTIPVLTDTIKYLWNYKRTVFTDGNETSTT
                     PVVIGVYGNKGDTGQTLYTWKMYADSEKGEGISPSPKGKRYFGLAVNRPTATPSTNPS
                     DYTWSSFFEGTVLGGRNYIDDYAMRETSFTNLQSEWRKEIVEDSTSVSGISIKMTCTK
                     AGSGGFKRDFFDLRTRIGQTMTFSVELKASRAVSMMIGSELGGTKPVSLTANWERVVS
                     SWKVSSNSTWSFVFTLSSGAWAQGDVVWLRNVQLEDGNVATAPGPSLADLMKQIDGKA
                     DQSFMKQQLDILTEKTESIRVDMEARALASEVSDWLKTYKEFEKSNKETLTKFNQDFV
                     TNTQRIAAIEADLKSNALLLQFVNTYLRAGDNGVIIGKKDNSEFIELTPQGMMIKSAG
                     NAVMTVTSGVIKIHHGVFVETLQVGYYRLEAARHNPKHLVCRFIDSKT"
     gene            complement(20811..21536)
                     /locus_tag="VHI09_000144"
     CDS             complement(20811..21536)
                     /locus_tag="VHI09_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ6120055.1"
                     /translation="MIQHNALIIDGVSTASFPYKILVEDRPSFHIPTSKSQLQEHDGL
                     SGGLVLTNKHRKAIEKAYRIHLIGATEEQINEFSALLTREGFWLESERMKMTRYWCYK
                     VDSFDIVQDNLGVFSMEVTFLCHPTRYFKKVSEYTLTKNGMIPLLGSALSYPKITVSG
                     KSTGETSFTIGEQVIGLEQLNKTLVMDNDPSHPSFLTESGELVHWSGDFITLDANQKD
                     KKVGVVLGTGITSLHIEILWGWT"
     gene            complement(21533..24652)
                     /locus_tag="VHI09_000145"
     CDS             complement(21533..24652)
                     /locus_tag="VHI09_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEQ6120056.1"
                     /translation="MAGNIKGITIEIGGDTQPLQRALKGINKESAESTKELKQIDKAL
                     KFDTGNVTLLTQKQEVLSKQIATTKEKLETLRQAQSQVEAQFQRGDIGAEQYRAFQRE
                     VETTQNVLKSYETKLEGVNRALDSHGNTVESNRSKLNSLEAEQAQLASESEKLNSTFR
                     LQESQLGSNASESEKLALAQRRIASQSELVERQIANLERQLELTKSEYGENSVEANRL
                     EKTLNDTKTAYNNLQQEMEGLSNASQQSAASLEQTNGLLKADILMEFGDRLGELSQKL
                     IEFGQQSLEAFREVDEGMDIIITKTGASGKALEGMQDIATELATSIPTDFATAGSAVG
                     ELNTQFGLTGDALSQASALLIKYSEINGSDVTQSAISAKQAIEAYGLESKDLAMVLDT
                     VTFTSQQTGVGVQDLMSKVVSGAPQIKSLGLSFDEGVALMGRFEKAGVDSSAALSTLS
                     KAAVNYAKDGLTLQDGLAKTVEQIKNSTNETESLTLASEVFGSKAAPRMVDAIKRGAF
                     SFNDLSGTAKNALGVVTSTYEATLDPIDKFTIAQNTAKAAMAEVGGVLAEALAPVLEL
                     LAQLLQAVANWFSNLPGPIQTFIVIMGGLITVVGLLLPGLLALQAAAVAMGTTIGGLV
                     VAAAPIVGTVLGIIAVITLLVVWIQELWQNNEGFRTAVIEIWNAIYAFISVIIQEIST
                     FIMTIWGTLTTWWTENQALIQAAVETVWNAISTVIQTVMSLIGPYLEAAWANIQLIIT
                     TAWEIIKTVVETAITVVLGIIKAIMQAITGDWSGAWETIKGVLQRVWQAIQQIVTTIL
                     SAIGQFISNTWNGIKNTISNILSAISGIVSSIWNTIKSVISSVISSIVSFVSSGWSGI
                     QQTISSILSGISSTVSSVWNGIKNSISNAINGAKNVVSSAINAIKNLFNFKISWPHIP
                     LPHFSVSGSANPLDWLKGGLPKISIAWYAKGGILTKPTAFGMNEKQLMVGGEAGKEAV
                     LPLTKQNLAAIGEGIASTMGTGGNFINVSITDVVIREEADIKRLANQVAERIHDELTR
                     QQSLRGVTL"
     gene            complement(24831..25250)
                     /locus_tag="VHI09_000146"
     CDS             complement(24831..25250)
                     /locus_tag="VHI09_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120057.1"
                     /translation="MRKQVTICGKVYPLASNAYTPIAYKEQFGKDYFQDLFQMINAKS
                     ILDQLNQLEEGEELTAQNMDVSILSDFDMTFFHRLFWVFAKSANPRVKAFDVFFMEME
                     ELPIQEIGPVLMEMLNQGMSTRKKSMKPMKRVKKSSR"
     gene            complement(25247..25837)
                     /locus_tag="VHI09_000147"
     CDS             complement(25247..25837)
                     /locus_tag="VHI09_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ6120058.1"
                     /translation="MAEKNKVTFGLQDVHWAEVTTEGSDGALTYGNVERLRGAAELTL
                     EATGDSGSYKADNINFYTTESNDGYSGTLKVALLTQEFLTRVLGETLDDTNKVITESS
                     NATRKNFALMFRFEGDKKETLHVLYYCYASRPTVGSKTKNGSDINEVELKFTASPRPL
                     DQVVRRRTTEETPETVKKNWFKSVYEPTVASGGSRT"
     gene            complement(25850..26167)
                     /locus_tag="VHI09_000148"
     CDS             complement(25850..26167)
                     /locus_tag="VHI09_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120059.1"
                     /translation="MSEKEFFKQLKALHLPIAYHHFEEGHSPSPPFMVYYSPQSENFG
                     ADNQVYHKGAQFILELYTRKKDLDVEGKIETFLTEQHLFFDKVETYIDTEQLYQVAFH
                     FAF"
     gene            complement(26164..26523)
                     /locus_tag="VHI09_000149"
     CDS             complement(26164..26523)
                     /locus_tag="VHI09_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018369002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ6120060.1"
                     /translation="MRADELSRAIEKELEEYLHQTTETVVEAVDDVTDEAIATLKSTS
                     PKRSGRYVRGWTSKETSSTSTGKTKTIHNRTPGLTHLLENGYAKQNGGRVSGRPHIGP
                     VEQKAVQALEKKIRGNL"
     gene            complement(26520..26849)
                     /locus_tag="VHI09_000150"
     CDS             complement(26520..26849)
                     /locus_tag="VHI09_000150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEQ6120061.1"
                     /translation="MKIAPLNQRVLFKKRVITQDDIGNETSQFTNLFSRWASVNQANS
                     DEIFEVGQTKRKLQLIVTLRYDSEVASLDTTNTQLIFQGKSFNILSIDHVFFEKKMVK
                     CRVEEEP"
     gene            complement(26850..27113)
                     /locus_tag="VHI09_000151"
     CDS             complement(26850..27113)
                     /locus_tag="VHI09_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEQ6120062.1"
                     /translation="MLTLLEAKIYLRVDTSEEDNLIKVLIATSEELCKDVLRVDELVD
                     TPLIRSAMLYAVAYLFEHREEANHHDLKETLYHLLGGLRKEVF"
     gene            complement(27117..28304)
                     /locus_tag="VHI09_000152"
     CDS             complement(27117..28304)
                     /locus_tag="VHI09_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEQ6120063.1"
                     /translation="MDKILELREKRAKVWDKAKNFLDSCRDEKGMISQEDSEHYDKLE
                     QEVVDLGKEIDRLERQERIERELSQATSAAIVSQPRTDAGGDEKTGTASTVYNQSFWK
                     NVRQKNFFDVNNTLNIGNDGQGGYLVPDEYENRLIKALEEENFFRRLATIIKTSNGER
                     KIPVVTGHGTAAWMDENGTYPESEETFGQVTLGAYKVGTAIKISEELIHDSVFDLESY
                     MAEEFARRIGSKEEEAFLIGDGSGKPTGVFTTVTEGVTSTGANITFDDVMDLYHSLKS
                     PYRKNSVWILNDSTIKALRKLKDNNGNYIWQPSVQAGVPDMILNRPYYTSSYAPEIAS
                     GQKVLAFGDFSHYWISDRQGRSFKRLNELYATSGQVGFLGSQRVDGKLVLPEAVKVLT
                     MKG"
     gene            complement(28320..29006)
                     /locus_tag="VHI09_000153"
     CDS             complement(28320..29006)
                     /locus_tag="VHI09_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEQ6120064.1"
                     /translation="MHKFWNWAKNEGERTLFLNGVIASESWFGDEVTPQLFKDELLSE
                     QGDITLWINSPGGDVFAAAQIYNMLMDYKGAVTVNIDGIAASAASVIAMAGTIVNMSP
                     VSMMMIHNPATFASGDKREMEKAIQLLNEVKESIINAYEIKTSLSRQKLSNLMDGETW
                     LNAKKAVELGFADQIIFDGTHDNDESQDAYAFSMQTVTNQVVAKCEQLIDKPKVAVST
                     LEKRLQLLKP"
     gene            complement(28999..30240)
                     /locus_tag="VHI09_000154"
     CDS             complement(28999..30240)
                     /locus_tag="VHI09_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ6120065.1"
                     /translation="MGILELIGLKRARDKPTNSYEGSDFSYLFGRTTSGKNVNEMTAL
                     QTTAVYACVRILAEAIASLPIHVYKHTDEGKEQDVNHQLYYLLHDEPNPDMTSFVFRE
                     TLMSHLLIWGNAYAQIIRDGRGQVLALYPLLPDRMTVKRDDKGELYYVYQRSEEDNPN
                     FKDKGNIILKKSEVLHVPGLGFDGLIGYSPIAMAKNAVGMTLATEEYGASFFANGANP
                     GGVLEHPGILKDPSKVRESWNQVYQGTNNSHKVAVLEEGMIYKTIGIPPNEAQFLETR
                     KFQINEIARLYRIPPHMVGDLEKSSFSNIEQQSLEFVKYTLDPWVVRFEQAFQKALLL
                     PDEKKTYFIKFNVDGLLRGDYQSRMNGYAIGRQNGWLSTNDIRRLEDMNPLSKEEGGD
                     LYLVNGNMTKLEDAGGFIKDA"
     gene            complement(30252..31844)
                     /locus_tag="VHI09_000155"
     CDS             complement(30252..31844)
                     /locus_tag="VHI09_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEQ6120066.1"
                     /translation="MTYHYKPSPFMLPTSHYDEKKADRAVAFIQNLKHTKGKWAGKHF
                     LLLPWQEQIIRDLFGIVKEDGNRQFLSAYVEIPKKNGKSELAAAIALYLLYADGEASA
                     EVYGAACDRNQASIVFDVAKQMVGMSNPLNKRSKIMGATKRIVNYNNAGFYQVLSAET
                     GTKHGLNVSGLVFDEIHAQPNRHLYDVLTKGSGDAREQPLFFIITTAGTDKNSICYEL
                     HTKALDILSGRKKDETFYPVVYGLANDEDWQDEENWYKANPSLGHTIQIDRVREAYRN
                     AIDNPAEENVFKQLRLNMWTSSTVTWIAEHVYDKGNRPIVESSLLGRECYAGLDLSST
                     SDITAFVLVFPPRTEDESYIVLPYFWLPEDTLPLRCRRDHVLYDVWERQGYLKTTEGN
                     VVHYGFIERFIEDLSTKFNIKEIAYDRWNATQMVQNLEDMGLTVVPFGQGYKDMSPPS
                     KELYKLMMEGRVTHAGHPVLKWMAQNVVMRQDPAGNIKPDKEKSVDKIDGIVALIMGL
                     DRCIRHKGDDGSIYDQRGILSF"
     gene            complement(31846..32313)
                     /locus_tag="VHI09_000156"
     CDS             complement(31846..32313)
                     /locus_tag="VHI09_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEQ6120067.1"
                     /translation="MAQRGRKPKPTGLKVLEGNLGKRPLNHKEPKPTKKAPRCPSWLE
                     EDAKKEWKRMSKVLESMGLLTDMDMTAFAGYCQAYARWKEAEEFLTKHGSIIKTPNGY
                     LQQVPQVSIAQTNMKIMLKFSEQFGLTPSARSRIQADSSDGEQDEMESLLREV"
     gene            complement(32379..32628)
                     /locus_tag="VHI09_000157"
                     /pseudo
     CDS             complement(32379..32628)
                     /locus_tag="VHI09_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32935..33147)
                     /locus_tag="VHI09_000158"
     CDS             complement(32935..33147)
                     /locus_tag="VHI09_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HEQ6120068.1"
                     /translation="MNNGAKERVECLYPVGCRVKLLEMDDPFPPPIGTLGTVYGHDDL
                     ASVLVHWDNGSGLSVVYGVDRIVKVE"
     gene            complement(33210..34478)
                     /gene="dcm"
                     /locus_tag="VHI09_000159"
     CDS             complement(33210..34478)
                     /gene="dcm"
                     /locus_tag="VHI09_000159"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEQ6120069.1"
                     /translation="MNQLTFIDFFAGIGGFRRGLELAGMTCIGYCEKDKFAVRSYQAM
                     YDTEGEWYSDDITKLKPNDIPKADIWTAGSPCQNVSIAGKRAGLRAERSGLFFTLVDL
                     LKSQSEEDKPEWLILENVKGLLSSRRGVDFLDYLLELDEAGYDLEWQVFNSKDYGVPQ
                     NRERVYTIGHLRSRGRRQVLPLQPESSGNLKQLVGGMQSYRVYDTDGISTTLVGTGGG
                     VGAKTGLYLIDQSTTKPKVTDQARCITARYTAGSTKRTAMNSGVMEVQPILTPDRVNK
                     RQNGRRLKEKGEPMFTLTSQDRHGVVEGIKVRNGTKQGYQVAELGDSVDLSYPASPTK
                     RARVGKGIAHNLSCSGQMGVVVWNGRMVKIRRLTPRECFRLQGFTDDLFEKAQAVNSD
                     AQLYKQAGNGVTVTVVYAIGKAILSCLNLR"
     gene            complement(34475..35725)
                     /locus_tag="VHI09_000160"
     CDS             complement(34475..35725)
                     /locus_tag="VHI09_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002904805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HEQ6120070.1"
                     /translation="MMTKESMTWLTLTVDQLNSASYNPRKALKKGDKEYEKIKRSIQE
                     FGYVEPIIVNYDMTIIGGHQRLTVLKDLGFKEVQCVQVHIEDENKVKALNVALNKITG
                     SWNEELLADLMLDLQEADFDTDLTGFEFPEIDQLFSKVHNKEVNEDDFDVDEELKKPT
                     VAKQGDIWYLGKHQVICGDSTLPETYITLMGDKKANLVLTDPPYNVNVEETAGKIKND
                     NMSDSDFYKFLFAMFVNVEQNMESDASIYVFHADTEGYNFRKAFKDAGFYLSGCCIWK
                     KNSLVLGRSPYQWQHEPCLFGWKNKGKHQWFSDRKQTTIWEYDRPKLSKEHPTMKPIQ
                     LMAYPIQNSSMRGTVVLDPFLGSGSTLIACEETGRICYGIELDEKFVDVIVKRYFEAT
                     GDQSIKVMRDGKEIKYADLVEVSV"
     gene            complement(35722..36126)
                     /locus_tag="VHI09_000161"
     CDS             complement(35722..36126)
                     /locus_tag="VHI09_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ6120071.1"
                     /translation="MNDFKAKQVWKMRNEGLGYRKIANLLDLSLTAVKRYCSTNPHLK
                     GYGQAVVAMISDGQRNGQLCKECLKPIEQPRKGRTKQFCSDSCRRKNWVTQHPEKADS
                     HVCELCGKTFTAYGSPRRKFCSRTCARTPRRT"
     gene            complement(36374..36739)
                     /locus_tag="VHI09_000162"
     CDS             complement(36374..36739)
                     /locus_tag="VHI09_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEQ6120072.1"
                     /translation="MPRRPSTPCKQNGCPNLVPYGHKYCDNHKANYQLDTKSTKAKGY
                     NAQWNKARLRYLKVQPLCVQCKVKGRLTKATVVDHITPHRGDQDLFWNQTNWQALCKS
                     CHDRKTKTTDRYVEYTYRF"
     gene            complement(36741..37778)
                     /locus_tag="VHI09_000163"
     CDS             complement(36741..37778)
                     /locus_tag="VHI09_000163"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ6120073.1"
                     /translation="MIISSEQVSVGHPDKICDQISDAILTECLKYDKSSRVAVETLIK
                     DNQVVVAGEISTRHYFNLENIVRQVVEPLGMKNVRVTNLLGLQSSDIAQGVDNGGAGD
                     QGMMFGYATDETPEYLPLPYVLATRVLEKLMSLGHPLLGKDAKAQVSYDYEKKRIDTF
                     LVSIQHTEIANLAKVKRIVTEAMMSVALRYRQNLDFKVLVNPTGRFVLGGSFADAGVT
                     GRKIVADTYGGFAHHGGGAFSGKDPSKVDRSAAYMARKIAKDIVREGYAKRCEVQLAY
                     AIGVAEPVSVYVETFGTSRYTSTQLEGMIRERYDLTPQGIIKELHLLNVDYTKTSCFG
                     HFTKAYLPWEK"
     gene            complement(37826..38008)
                     /locus_tag="VHI09_000164"
     CDS             complement(37826..38008)
                     /locus_tag="VHI09_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120074.1"
                     /translation="MTKRQQEKLNALLTEIAKEELFVETLEKRWSDNLDFYDVSVWGI
                     KRALERAYEAGQKSVK"
     gene            complement(38097..38324)
                     /locus_tag="VHI09_000165"
     CDS             complement(38097..38324)
                     /locus_tag="VHI09_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120075.1"
                     /translation="MWTDGRIDYQGQKVDYIAKVSPQPSEVGIDLGCIFKLDIEVAEE
                     TIVSYDRGWELYPETEEREAILEAVLMVLTV"
     gene            complement(38510..39013)
                     /locus_tag="VHI09_000166"
     CDS             complement(38510..39013)
                     /locus_tag="VHI09_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120076.1"
                     /translation="MDKVEYIAKNYRDMKMKLHLVQEKLLNYRPISENSVIQSLVFEK
                     SEHEKVKKSKNHGRSELISLSFREKQEQENQEYLSSLLNTYYCLKMDLYYFEFVMELI
                     PEDLKPLSKDLIYLGKSWTELEEIYEISHSTLAYRRRKILKQLRKCYRWTSKSLELKV
                     EDYHIPI"
     gene            complement(39006..40382)
                     /locus_tag="VHI09_000167"
     CDS             complement(39006..40382)
                     /locus_tag="VHI09_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006268649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ6120077.1"
                     /translation="MKLTLHNYQVVAKDFIIGHPYAAVILDMGMGKTATTLSAVNELM
                     FDRFEVTKVLVIAPLRVANTVWSDEIEQWSELRNLRYSKIVGTPKQRKVALQKDADIY
                     IVNRENLPWLVEQCSPYFKWDMVVIDELSSFKSWQSKRFKAFMTMRPYMKRVVGLTGT
                     PSSNGLMDLFAEFKVIDGGERLGRFIGEFRSRYFEEGRRNGNIVYEYIPMDYAECQIQ
                     DKISDITISMKALDYLDMPELISTKKLVRMSEKEKEKYIQFKKEYVLSELDGLEVTAA
                     NAASLMNKLVQLSNGAVYSDDHTVVPLHEQKLDALEDILESANGEPVLVAYWFKHDLA
                     RITGRLEKLKVTSRVLKTEEDIREWNKGNVPVGLIHPAGAGHGLNLQKGGHHLVWFGL
                     TWSLELYQQTNARLWRQGQEAETVVIQHIVTEGTIDEEILKALGNKDAQQERLIEAVK
                     AQVGGTDG"
     gene            complement(40363..40644)
                     /locus_tag="VHI09_000168"
     CDS             complement(40363..40644)
                     /locus_tag="VHI09_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEQ6120078.1"
                     /translation="MREKYVEQALVKSVKARGGICPKWVSPSFSGVPDRLVFLPKGKF
                     GLVEVKAPDQKPRKLQVSRHKLFERLGFKVYVIDRIEMIGEVLDEIDIT"
     gene            complement(40929..43214)
                     /locus_tag="VHI09_000169"
     CDS             complement(40929..43214)
                     /locus_tag="VHI09_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ6120079.1"
                     /translation="MKFTLSHSGQTGIQTTTVYPNMVTITDKAILQNVAQFDHVAGLF
                     VQNTRSNANFIKSDVLVMDIDNDHTENPDEWLTEEFLKDVFADYNFALVTSRSHMQTK
                     AGKAPRPKFHIYFQINEVTDKDIYVAMKEELCNQYKFFDDHAKDAARFFFGNPNAQVI
                     WHDSWLTIDEDLFQVVSIEDEEDFDADFYTPPSGPIQQGSRNSTMSVFAAKILKRLGV
                     TQEARDGFDEQAQKCVPPLDKAELDTIWGSAVRFYNRTIKTSKSYVAPDAFNRETLKP
                     DDYSDVGEAGVLAREYANKLAYTNATDYLYYDGTHWRENKQLALGAVVHFTDEQLAEA
                     IALLESADKQLQSSGIDELTIKAGGKRLENAVETPLQLKYLKAYLAAKEFHKFVMKHR
                     DYKNLMAVYNTAKPILSVELSELDSDDLLLNTPEATYDLRKGINGQQEHNPEDYITKI
                     TAVSPSDQGMGLWQETLATFFCNDQELIDYVQEIIGMAAIGKVYQEHMIIAYGGGANG
                     KSTFWNTIARVLGSYSGKLSADALTMSNKRNVSPELAELKGKRLVIASEMAEGMRLNT
                     AVVKQITSTDEIQAEKKYKDPFHFVPSHTLVLYTNHLPKVGANDDGTWRRLVVIPFNA
                     KITGRSDIKNFADHLYDNAAPAIMSWIIEGAEKAIKANFKTNVPTAVSSSVKAYREAN
                     DWLGHFLGDCCQVGDQLSEKSGELYSQYRAYCAKNMEYTRSTTDFYSALEQAGFKRKR
                     TSKGNHILGLKLVEDGYDFLD"
BASE COUNT        12200 a         9696 c         7823 g        13614 t
ORIGIN      
        1 taaaacaata gaatagcgtt aatctgaagc gccttactca actattggtt ttatttaagt
       61 gagtgaccac acaataaaaa agaaaaaaat gaaaccgtca aactaaggtc aatgaagcct
      121 gcttttcagg actgataatc cactaaaata taaaaagaaa atgaccgata gaaatggaga
      181 ttcacaccaa ctgtgaatct ccatttctat caatcctaaa ctctttttgt ccttgacttt
      241 tgtgatttta gcttttgtgt atgcctttgg acacgagtac ttcaagttct gttagacgtt
      301 cgtcaaagac tttaaaggcg gcttccaaat aatcaccttt tgccatatca acgcctgctt
      361 tagcaatcac ttcaagtggg taatcagaat ttcctgactt aaggtaggcc aagtaatgat
      421 cgatatcatc ttgtgtgcca tgcacaatct tatccgctaa atagcttgca gctgcaaacc
      481 ctgttgcata ttggtaaaca taataattgt aatagaaatg aggaatacgt gcccattcat
      541 actgaataaa atgattatct tttttactta aaccataata tttttcattt aaatcagcgt
      601 atagctggtt aaggtattca cttgttaaca cttctccttt ttgatcagct tgatgaatgg
      661 catgttcaaa ctcagcaaat tgggtttgac ggaaaactgt tccacggaaa ccatcgaggt
      721 aatggtttaa aatggcaaag cgttctttct catcttgaac ctcatttaag agagcttctg
      781 tcataatatt ttcattcgtt gtggatgcaa tttctgccaa gaagatacta taatccccat
      841 acacataagg ctgtgtttca cgagtgaaag tagaatgcaa actgtggcct gtttcgtgga
      901 caagtgtgta aagattatca agtgtatctt gccagttcaa cagcatgaag gcattggtat
      961 cataggaacc acctgaataa gccccagagc gcttcccttt attgacgtga acatcaatcc
     1021 aacgttcggt aaaagcacga tgtacacgat ctgcgtaatc cttaccaaaa acagccaaaa
     1081 ccttctcagc cttttccaaa gcctcatcat aaccaatggc taaatctgtt tcagataatg
     1141 gcgtgtaaac atcatacatc ttcaaatcgt ctaaaccaag aacctcttga cgcaatttca
     1201 aataacggtg taagagtggt aaatgtttgt taaccgtttc taataaagtg tcgtaaacag
     1261 cttctggaat aaagttagca gccatggcag cttgacgtgc tgaatcgtat ttatgaacac
     1321 gcgccttata gttttgcact ttcacattgg tttgcaaggt tttagcgtaa gtatgttgga
     1381 actgttcata agtactatac atggcttcat aagcagcttg gcgaacgcta cggtccttag
     1441 attccattag gctaatgaag ttaccatggg ttaattcaac gttttcacct ttatcatttc
     1501 tgactatcgg gaaaacaatg tcagcgttat caagaatact aaaggtttct tccgcaccat
     1561 tgaaaatttc ttgagctcct gctaataatt cctcttctgc ttgactaaga acatgttcgc
     1621 gggcttggaa taatttgtca aagaaatggt tgtacacttt caactcaggg gtttcagtaa
     1681 ggaaagcctg atagtcttct tgatgaagca tcatcacttc tggatcatag aatgaaaaga
     1741 cttcgctaaa ctttgcatac aagcctgaag ccttagcttg gtattcttga tacttggcaa
     1801 cagtagtgtc ttggtcattt ttcatgtgag cataaacata gactttctcc acacgacgag
     1861 ccaattctaa atatgttttt gtaaccttta acaaattagc actagaatcc agcaaatgac
     1921 cagcaaaacc tttagatgcc tcaacttcag tagctaagtc actgacttca gcttcccaat
     1981 ccttatctgt tgcaaaaata gtgctcaaat cccaagtgta tttttcttct aaatggctgc
     2041 gattatcagt cataacatct ccttctcatt ttctattact atccatttta acataatatt
     2101 ttggtgaaaa aagcgtttgc accttcttat ccttttcttt tttgtaatag actttaaaac
     2161 tttcataata agggcgaaga tttcctttaa tttgacaaaa accgctggaa gattgaactg
     2221 gtctccattg aggataaaaa gcatcagttg ttaacataag taaattatag cctgataagt
     2281 aagccttttc ttgtcgtcgt agccatttcg ggtgtctagc taaaagagct ctttgtattc
     2341 ttttagatag gttcacagtc atttttttct gataagaata aaggatttct tgctgatagg
     2401 gtaatcgaaa catttccata atattgtggt ctaaagaaat ggttttggtt aggtaactta
     2461 ccttaccaaa taagtcttca tggattaaat atttgagacg tagcagattt gcagctgcat
     2521 ccagctccca caaatgaaag cccaaactag atgaaaagta aagaaactgt ttttgcaaag
     2581 cagttaaatg agtgttaagc cacaattttc ttcccagtaa ccatcgtacc ggatacccct
     2641 tttcttgata ggctttggtg cgtttcttta gacgctcaac tggcaaggga ctgcactgaa
     2701 tttctaaggc taacttatca ttcacccaaa ggtctgctat ctgttgcagc tcaggaagat
     2761 atttttcaat acaaacagct tctgttcgga ctaaactggt gtaaagtttt gccttcaacg
     2821 tcaaatgttc ctcagattca ttttctgctt ggaattgaca atgagctagt tgtacatggg
     2881 caaaatgagg tcgtctaatt gtcccctgac gcaagcgaac aggagatttg caagctgggc
     2941 aacgaaaagg cggtttcgtt gaaataggct gagttactag cgaaatgagc tgattcttac
     3001 catctaaagc ggttaatatt tttgtcacct cctattagat cttccacctt attattcgaa
     3061 aactaagtac caaactttaa gtagctttag tttttttagc catcaaaaaa cactcccatt
     3121 tccaatttat gagagtttaa cacaagggtt tttataacgc ttcaggttaa ggagacagtg
     3181 ggaattgaat ctttttgctt acctgatttt gtaacaacaa gatgactaaa catagcaaaa
     3241 gtcggactca tcatgactat aatagtcaat ggacacttta ttgattaagc aagaatagct
     3301 tctttttgaa agcatgtttt gctcagtcta gatacgtttt aatgagttgc ctttctgact
     3361 ccgttagata acgatattct ccgctggcta aatgaccgag ttccagtcct gcaaagtgcg
     3421 tccgtttcag agaggtcact ttcaaaccat aggtcaaaaa catttttttc acttggtgaa
     3481 attttccttc tgaaatggtc aaactagcct gtgactggtc tgtatcggct ttaagaatag
     3541 tcaattgagc tggttggcat tgttcacctg ttgggaaaca aataccagct gcaaaaaaat
     3601 ctgaggcatc ttctgctaac aagccattaa cggttacaag atacgtttta gaaacatgat
     3661 gaatagggtg caacatccga aaccctaggg gaccattatc agtgagtaaa accaaccctt
     3721 cggtatctct atccaatctg ccaacaggat acaagtctgg acttttatct tcttcagcaa
     3781 gctgatcaat aacggttaaa tagttagtat ctttcttagc tgagaccacc cctgatggct
     3841 tgttgaggat aatgtaggaa tgtttaggat gcgtcactcg ctgtccagtc acctcaatca
     3901 gttgcaagcc aggatcaaca ttttgtcgac catttctggc aggcatgtga tcaacccaaa
     3961 ccccttgagc cttaatcagt ttcttcactt gacttctaga gccaactttt gtaccttcta
     4021 ataatttatc taaacgcata ttagtagtgt aacataaatt tacccttgca aaagaaatag
     4081 aagttacaag acttcttctt aaatgaggtt gactatctcc tcatttttgt tcttacaagg
     4141 tttaacgact ggttaggtta gtctaattgt gacgcagcag cttcatccac aatgacgata
     4201 acatgatcat gtttttggag aatacttgct gggaggtctt ctgtgattgg tccaaaaacc
     4261 attcccttga tcgcatcagc tttttcttgt ccaaaggcaa gaagaacaat cattttggac
     4321 ttcatgatgg aggcaattcc catagagata gcttgttttg gcacatcctc aatacttgta
     4381 aagaaacgac tattggcttc aatggttgat ttttgaagat ccaccacatg cgtttcttct
     4441 tcaaaagagg tcccaggttc gttaaaacca atgtgcccat tacgcccaat cccaagcact
     4501 tgaaaatcaa tcggatgctc tgcaatgatt tggttatatc gtttagcttc agcttcgata
     4561 tcagttgcta aaccattggg caagtaattc tttttaaaag gcttcgcatt aaaaaggttt
     4621 tgacgcataa aataatcata actctgatcg ctctctactg aaagaccgac atattcatcc
     4681 aagttaatac ttgttaagtc cgaaaaatcc aaaggacttt tgaccatttc ttggtaaaaa
     4741 ctaattggtg aacttcctgt tgctaatcct aacgtcttag ctcccttagc taagctatct
     4801 ttcaataaag taaaagcgat tttcccacct tcaatctgat cttggacccg aataattttc
     4861 atgtcattct ccttttctaa ttggtataga cctttcgtgt ttttattata tggtatatac
     4921 caatttttgt caagcctatc ctttactttc tgatgaaaga ttaaagatga cgggttagac
     4981 gacaagtgat ataatagaaa agatttatct tacatgaaag agttactagg aagagattta
     5041 ctagtggctt taaaggagcc ccaatgaata ctaacgattt tgattttgag ttaccagaag
     5101 aattgattgc ccaaacccca ctcgaaaaaa gagacagttc aaaactattg attattgatc
     5161 accgtcaaaa aactatggtt gatagccatt ttgaccatat cattgatcaa ttaaatccag
     5221 gggatgccct cgtcatgaat aacacccgtg tcttgcctgc cagactttat ggagaaaaac
     5281 ccgatactca tggtcatgtg gaattgcttt tgcttaaaaa tacacaagga gatcaatggg
     5341 aagttttagc caagccagcc aaacggctca aggttggtag ccaagtcaat tttggagatg
     5401 ggcgtctcaa agccaccatt atcgacgaat tagaacatgg tggacgaatt gttgaattca
     5461 gctatgatgg gattttctta gaagttttag agagcctcgg cgaaatgcca ttgcctcctt
     5521 atattcacga aaaattaaag gatgctgagc gataccaaac cgtttatgct aaagaaaatg
     5581 gctctgcggc cgccccaact gctggtctcc acttcacaac agacttgctc aaaaaaatcg
     5641 aagccaaagg ggtacatttg gtttatttga ccctacatgt agggcttggg acttttagac
     5701 ctgtttccgt ggataacctc gatgagcatg atatgcattc ggaattttat tccctctcag
     5761 aagaagcagc gcaaacccta agagacgtca aacaagctgg cggtcgtgtt gtagctgtag
     5821 gaaccacctc tattcgtaca ttggaaacca tcgggagtaa gttccaaggg gacattcaag
     5881 ctgattcagg ttggaccaat atttttatta aaccaggcta ccaattcaag gtagttgatg
     5941 ctttttcaac taatttccat cttccaaaat caacccttgt gatgctagta tcagcctttg
     6001 cagggcgtga ctttgtcctt gaagcttaca gacacgcggt tgacgaaaaa tatcgtttct
     6061 ttagctttgg cgatgccatg tttgtcaact aaaaatctat gaaattactt ggaaaatggc
     6121 tattcttctt gatgtttctg actttttgcg tcagtttttg gcagaaagac tatcagcgga
     6181 ttgcctttga aaaagaacac aaactttatc aagatttagt ggcacctttt taccaaaact
     6241 atcctaaatt acgagaactc cagctttcag aagctattaa attacgccac gacttgcttg
     6301 attatgttcg aaagctcgat aaggacggat ggtcctataa agccatccaa aaaggatacc
     6361 ttgaacaact cgctgttaga ggaaactctt atcatttcga acaactttat agtcgcattc
     6421 gcctgatagg atcacctgac tttcaaaagc tctggcaaca agaagagatg gcacagacgc
     6481 ctcaagaagc tcaaaaacgg ctacagctgc tcttgacgta tttaaagatg ccagacgagt
     6541 tgacaggaca atcaaaacaa acacagcaag tattggcaca attaagtcca aagctatcgc
     6601 caacagatcc tttttgggac caattatctg ccctgattca ggcttgttat gttaatctcg
     6661 aacacattcc ttatagccgt tttaatcgga aaattcatca actccgttac ctcatatcaa
     6721 cccagcaaat ggagtgggtt cgctcacatt atgggaataa agggaaaacc gatgcggatg
     6781 ccttagccaa atatttggct actctagaag atgacgatta tgctctttat gaatcttcta
     6841 gataccataa taaggttgct agtattttag atgctaatgg caatcatcag gcagtctata
     6901 cagacaatat tcctcaagcc aactataaga ttttgattca ttttcactca gagtttattt
     6961 tgtcagaatc tggacaattt ttagtagcac tagatcctga taacctaacg cgaaatagta
     7021 ttgtcaatgg ttcttctttc aattatggca accaaaacga tgacttgcac cgtctgcttg
     7081 atattgaccc tattctgctt tttgatccgg cttttattga aaaagcaact aatagtcctg
     7141 atgcaacttt tctagttccc gatttagaac agcaaaggga taagcacaat cccatttaca
     7201 gcagaaatgg caaatcatct aagcagctaa cacgcgctgc cgttaaaaaa ttcaaaaaat
     7261 tgcttcacca ttatcaagaa acaagaggtg acaataagac ctcaaaaaca cagcattaat
     7321 ctcaaaaagc ttgacctgaa gaaacaggcc aagctttttt aatgagcaaa aacttatttt
     7381 agtgactttc atcagtaagt atgtctacta tcagactaat gtcaactgag tattagatca
     7441 tgttgtgatt gactctatgc cactcattaa ttacatatgg tagctgaaca agctgcacat
     7501 aaccaaggcc attgacttcc tctgatgctt gtgttgctcc acccaaacca gatgaataaa
     7561 gagcaatcaa ataaggagac tcttcgcaaa caatggcacc tacatttaag gcttctctga
     7621 cataacccgg tttttgataa acctttacat gaggcaaata agtcttgtaa tacaaatcag
     7681 ggaaagattc accaatgtag tacaagatat ccttatattt atcctgatgc tgccacaagt
     7741 aatccaaaac ttgcaaatag taagcggtgg tgattttatt gccattacga tcaatggtcg
     7801 taatagcccc ggataccttc ccgtagcggt caaataattg gtaagcctta ttcatgccac
     7861 ccaatcgttc tgctagagca taagctggcg tgttttctga gtacaccaag gaatattctt
     7921 gcatatccgg aatagacatg gcaccattga attgagcaac ataggcatga tgttcctgat
     7981 catattcata atcagtttct gtaatgtcaa aacgctctgt catggataat ttgccttatc
     8041 taccaccagc atgtttaatg gcagcttata ggtgcttcct gcaatcattg gctgatggtc
     8101 attcatggca acagtcctac ctgaattgag gtctttgtag gaaaaggcaa tttgggattc
     8161 accaatgcca aattccttta aatacgcctt gaccgtattg accaaactca aatggttgtc
     8221 atcgtaatag agccctaagg catccatttg cactaaatct gtatccataa cagcttgctt
     8281 agcctcttta gatttttcag gtaacggttt ttaaacagtt tttctaatct tgcttttcag
     8341 ccgtttgctg ccgcttacct gctttaggag gaaagccttt ctttttacca gtcaaacagc
     8401 cgacaagtaa ggtccctatc atcattccta aaataatccc tacaatggta gttttcttct
     8461 ctcttaacac ctaaaatctc ctatccgttc tattctagct aaatttagct aaaaatcaag
     8521 gaattacatt aaaaagacaa actattttta tcttttctaa aaaatgatat ccccacactg
     8581 ttattcacaa aagcacttaa aaaaccaatc tctttttgag attggaaacc cttttcttat
     8641 ttagcggctt ggtaaagttc tgatactttt ttccaattaa tgatttcaaa gaaagctttg
     8701 atataatttg gacgtacatt acgataattc aagtagtaag catgctccca cacatcaagt
     8761 gctagaattg gttttttgcc ttctgaaatt ggagtatctt ggttagcggt tgatgtgatt
     8821 tcgagttgtc cttctttatt aacaactaac catgcccaac ctgaaccaaa acgtccagta
     8881 gcagctgctg taaattgttc tttaaaggcg tcaaatgacc caaaagcatc atcaatggct
     8941 tgtgcaacat caggagtgac gtcttgtttt tcaggtgata atagttccca gaaaagcgca
     9001 tggtttaggt gtccgccacc attattaatt aaggcttgac gaatatcttc tggaatctta
     9061 gtgacatctg ccaatagttc ctccagattt tctccgatct ctgggtgttt ttctaaagcc
     9121 gcattggtat tagcgacata agtggcatgg tgtttatcat gatgaagagt cattgtctcc
     9181 gcatcaaact gtggttcaag agcatcatac gcgtatggaa gttctggtaa aataatagcc
     9241 attttcctgt cctcttttcc tttatgatac gtctatctta tcgcaaaaaa taagagcttt
     9301 caactaattt gattatctct tggaaagcgg tccaatgcct atttttgaga gtgagtcatg
     9361 atttttaaga gagcaatatc aactagataa ctcttctcat aaagtcctgt ttttatctgg
     9421 taatctgtct caatcaaggt tttcaccgct cctgttagaa aggcaagaga taaggtccta
     9481 gaatccttta acgcatactt gacctggtaa ggattaaccc gccgcccaag aatatctgat
     9541 aaactaatca ctagttgttg ctcgtttttt acgtctctag caagaatagt cagctgcaaa
     9601 aataaacgaa attggcctag catgatagcg attaatttaa tgtcatcttc tccagataac
     9661 cgtaaatcat gaatcaaatc tctagccgca tcaattttac ctcctaggac aagtctagtc
     9721 agatcgaaaa tattatcttg taaactttta ggaatggctt gctcaatatc agttaggcta
     9781 atatttcccg tttttttata ggcttttaaa aaggccatgt ttttcatgat ttgactaaaa
     9841 tcatcgtttg atttcaaaag taattggtca aaggcaccac tctcgaaacc taaacccagt
     9901 tgatgactgt atttttgaaa ataagttctt agctctgctt ctttcagagg gttggcttct
     9961 aaaacaaggg catcacgttt caaaagctta acaagccgtc tcttactatc caatttacct
    10021 ggagcaaaga taattagtcg agtagtctct aagggatttt ctaaataggc ttcaaaggtc
    10081 tttaagtctt tttcttttaa gaaacttttt ttattggtcg tgatatctaa caaatggtca
    10141 aaaatgacca ccttctgctc agcaaagaag ggtaggctca ctagatccat ttctgcatcc
    10201 tgataagcgg cctcagacat atcaaagtaa gaataggcca aatcatcctt atcaaaagca
    10261 atctgctcca ttaagcggga tttcaactgg ctatactgac caatgtcatc tcctgtgaca
    10321 agggttataa gacccaaatt ttctttactc agtttttcaa tcttttctat cgcaatcatg
    10381 cttttattat atcatctttc tcctaaaagg aaagcgactg cctgtttctt cgtcaggctg
    10441 gaattttaac taacggaccg tttcaagatg ccagcttgtc caaccagtca aacgaatagc
    10501 tccttgcgta tccgttcggt aataagtaat ttgtctgtct tccaaacggg ctaaggtttc
    10561 tctgtgagga tgctgataac ggttattttt tccagcagaa ataaaggcca ctttaggctg
    10621 aatatggtct aaaaaagctg cactagaaga ggtatttgat ccatggtgac ctgcttttaa
    10681 ataatctact cttagttgag gataacgttt tatgatctcg ttttccccct ctttttccaa
    10741 gtcacctgtg aaaagaaaag ttcgatttaa gagcctgcca taaagtacca aagaatcgtt
    10801 atttttcccg tcccctaact gccaaggata gagtacctgt aagacacttc ccataatagg
    10861 caactggtct ccagctgcta aaaccctaac gtgacatttt aaacgcctta gccgcctaac
    10921 aaagcttgga tggcttaaac tgccttgact agtcaaaatc tcctttatcc gaatagcttt
    10981 ggcaaccact tcgatatccc ccatatgatc agtatcggtg tgagttctca atttgataca
    11041 agctcaataa aaaaatgaaa ctcctaattg agtttcattt gagtttcatt tttttcttaa
    11101 cttattaatc cgtacccact aactcacaat ttatataatc atcaatcgaa atcactttta
    11161 acttggctcc tttttggata atgacttcat aataatgtgt ctcttcatta ttaagatttc
    11221 ctacataaaa tgcgttagtt cctttaggaa ttttaatacg tagtttttta taaggatctc
    11281 tgaattcaaa tcctttgaca agagaggtat gaaggaagcc tttctcttta aaatcaatac
    11341 atccttccac ttgtgctgat tctaccattt tttctaatgt ttccatacta actcctcgat
    11401 atactacaat atctgctttt gtgctatatt tttttagagt ttggtaaata ttttctatct
    11461 tttcatcaac agaacctgac ctcaatctac catttatggt tggcccagaa gaagcaataa
    11521 tatattcgtc agatggtatt ttccccacat accttggtgg ctcattattt agatactttg
    11581 cgacaaagag gggtacatca gcttgggaac tcgaaagcaa atcccatttc tggccagttc
    11641 taagtatttt tcctttaagt ttatagcaaa actcgttcat aagactttat tcttacctac
    11701 tttttttaca cacattatat caaaatttca tagaattact ctactacctc tattccattc
    11761 ttaaatttta tagccactgt ttggtcacaa aaaattttaa tttcatctac catagtttcc
    11821 caaagcaact catcaaactc acttataagc tcactttgct gattgagtgt ctttaaaaat
    11881 tctttgaggt ctttttgatg actggctgct tccagtagct gcttctctat cgactcaatt
    11941 tcactaacca gtgcatcatg ccgctcgacc aagttactgt actttttctg ataggtatct
    12001 tggtcctgtg gcattatcgc attctctttc actagattag aaaccatttg agtgactact
    12061 tctacctcgg tttcaagctc aaccaacctt cgttcaaggt cagaactttc cccaacgtca
    12121 agtaacattt ctagattctg gataatctca tccttattgc tgattacctt atttattgct
    12181 gttacaaacc aatccttaat ttcatcttca gtaacatgag gagtggtaca cttcttttcg
    12241 cccttgtact tctcattaca ttggtagata acccttcgat attttgatgt ggagtgccag
    12301 accttgctac cgaagtaatg cccacaatcc ccacatacca gctttgaact aaatagtgta
    12361 gtactcccct tacccttttc tttcctatct agtgatgctt gaactgcatc aaacgtttct
    12421 ttatctacaa tggcttcatg gttattttct acataatact gtggtaactc tcccttgtta
    12481 atgttcttct ttttggatag aaaatcaatt gtgtaggttt tctgaaggag ggcatcaccc
    12541 ttgtacttct cattccttaa catccgtttg atggtcacag aactccaatg attcttacca
    12601 ctaggtgaga ggataccttg ttgtgtcaag tcttttgcaa tgctataggg agttttacca
    12661 agcaatgcct gttggaaaat gtagcggaca gtcttagctt cttcctgatt aacaacaatc
    12721 tcaccattct cgcccttttc aaagcccatg acgttggtat agggaaaatg tacctttcct
    12781 tccgcaaact gtttcctcaa gccccaagtg acgttctctg agatgctacg gctttcttct
    12841 tgggccaagc tagacatgat ggtaatcaac agctcaccct ttgaatctag agtccagata
    12901 ttctctttct caaagtagat ttcaactccg acttccttca acttacgaac tgttgtcaat
    12961 gaatcaactg tatttcttgc aaaccgacta actgacttgg taataatgag gtcaattttg
    13021 ccagccaagg catcctcaat catgctctta aaacccactc gcagtttagt attagtgcca
    13081 ctaatacctt catccgagta cattttaaca aactcccaat cgcttcgagt tgaaatgtat
    13141 tctgcataat acctcatctg agattcatag ctggttgttt ggtcttcatg atcagttgat
    13201 accctcgcat aacctgctac acgacgtttc ttaggtagcg aaatgctagc taactctgag
    13261 ttcctaggcg catttgcttc aatagttatg acgtttttca taagaagtgc tcctttctaa
    13321 ccttcagttc atattttcta ccttttatag gaaagacggt aattaaacct actgatgagt
    13381 cataaataac cttcttgata ttttcggtaa cccaattttt atctggttta aatccaattt
    13441 tatcttcaaa tgtagctaat atccgttttt cagatagagt cttactagga caagaggatg
    13501 ttccgaacct gtctctagtg cggcaacagt atctgatggt tcggtgtacc tttttggagt
    13561 ctactctagt aatcattatt tttccacaat ggctacactc gattaactct ctaaaagtac
    13621 ctttgtgcat atggctttcc tgatacatta ccttgtaccg acgttctttt tcttgtagta
    13681 ccacatcaaa ataagacttt gaaacaatgg cttcatgagc atcctctaca atatacttat
    13741 tcttttgccc attatttcgt ttaggatttc gtgaaaaagg ttctcggtaa gtcttctgta
    13801 ataccaaacg tccaaagtat gcttcctgct taaagaactc tcggacacta gagactgtga
    13861 agggatttcc cagtcgtgtc aagactcctt gctcatttaa ctttttagta atcattgggg
    13921 cattatctcc gtccaagaac cattgaaaca cctgtcgtac aacctttgct tcatcaggct
    13981 caatgacata attctcacca ttccaacggt agccatagat atcttgaggt gtatgaggtt
    14041 gaccctgttc aaactttttc ttaatctgcc atcttaagtt ttcactgatg ttatgcgatt
    14101 cttcttgagc gacagaagct aggagagtta acatcaactc gccttcggaa cttaggctat
    14161 ctatcccttc tttttcaaag gtaattccta tgttcttttg tctaagtaca cgcactgtag
    14221 acaataggtc aacggtgttt ctaccgaacc ttgcaatgga tttagttaaa attctatcaa
    14281 tttttccatc atcacatgcc tttaacaaat cttgtaaccc ttgacggtta ctttgttctt
    14341 taccgctgat gccttcatca taataaacgc caaccaattc ccagtctgga tgagactgaa
    14401 ttaatctact ataatagctg acttgagtgg ataacgaatg atgtaggcga ctatcagata
    14461 ccctcgcata tgctgctaca cgcaacttag gtttttgcgg tgtcatcatc ggttgaattc
    14521 gtctaactat tctcaatttg atacactcct ttcgctacta tatatcactc taaaggcctc
    14581 atttatcaag tctttaggtc gataatctac taataaatgg ttgatatttt tcaagcatct
    14641 tttccttaaa ttgctgatga acttcctcac taataatcct agattttagg agtctatcgg
    14701 cctgtatcat ggtcagttgg taagtcatct catcttgtaa tttgtcatta gtcataatag
    14761 ccctcctacc ttactaagta aggctgaggg cattttttcc gctttttagg caaaaaaaat
    14821 aagcctgaca gaaaatacac tccatcaggc ttaacataag ttactgttta attgcctttt
    14881 ccaaggcctg tactgaagca accaatttct catcgtcaaa cttttgtgac tctaagaaac
    14941 ggttgaaatc gccatcgtct aaggttaagt gtgctgcacc agctgattgc aactgaacaa
    15001 ctgtatcaat gtgaccaatc ccaaagacag caccattgac cactgccaca taaccttgtt
    15061 tcccactttt tgatcgtacg acaaaatttt tcatatcctc atcctccgaa gtttctactt
    15121 tctttgtgct agttgctacc tcataaggca gtctaaacca gccaaccata taaccaacca
    15181 atccaccact atggctatca actaatcgcc catcagaata ccactttcgt gacacacgtc
    15241 gagtgtaacc gccaacttcc agttgatcgt tgaccccatt acggttacta tccgaataac
    15301 catcgacatt ttgttccaca ccttcaagtc cagtaccatc agaatccgct aaacaaacac
    15361 cgatgtgacc aaaactgtga ctcggtacac gaatcacgta aaagtcaccc gcctgtgggt
    15421 taacccctac ggcatcatag gtgacctgaa aaccattggc ttttgcctta tcaagacagt
    15481 caatggcatt ggtgtaagac atattcttcc ctgtttcctc ctgaacgatt ttatcaatta
    15541 gagccacaca ttgcccacca taaggattgg taggcaccgt cactttggta ttgactttcg
    15601 agagggcatt ggcaacaacc ttttctttac ttgtcattct catcctcctt atcttcgttt
    15661 aactgacgta agacttgttt aatacgatta ggcactggca atccaatacg agtcgcattc
    15721 tccaagatgg acagtccttc attactgaag tagaaaaaga taacagctgt tcgaactgcc
    15781 ccaccttgtt ttaagacatg taggtcaatc atgtgggcaa cagccaccaa acagataatc
    15841 atggcttttt tgaaaatccc ttttttaccg atagaactgg agaggttctt ctcgacaatg
    15901 gctgccataa tccctgtcac atagtcaatc aacataaaaa tcaaaaggcc ataaagaaag
    15961 ccatcgattt ctccaaaaat agaaccgatg accccaccga ttcctgaaaa cactaattta
    16021 ttagtcaaaa cttcttgttt cattgtttct cctctaagcg gtacgtcgcc accgataaac
    16081 tgtaatataa ggttgtaaat tgttatgagc ctgtcctcct ccagaattac ctgtgttatt
    16141 tccttgagga tacaatgatg aactcccgtc agaagaataa tctcgacgtg tggcctgatt
    16201 acctgaattg gcagaaacat attgcggatg tgagtgaaca ggcatttcat tgatggttaa
    16261 ggtatgagtt ttgctgccac ctgtttttga aacagtatta aattcgcttt ctgtctcaga
    16321 gacaccaacc aaaacacgtc cattcccaaa ccgctcccac ttccctccca taaaagttga
    16381 aggactttca gaactcgtag actcataaat cgttccaatc ggatagaaaa tatcaatcag
    16441 ctttttgtta cgcatgatga tgtctccatc aaaataagaa ggctgactac catccacatc
    16501 aagaacccct cgtgtccatg cttttcctat ccccatccct gtcggagata agccataaac
    16561 taccttttca ggaccaaccg taaactcaaa atccgtttca tagaataaat ctcgcaaatg
    16621 accaatgatg gtataagact tggtttggtc ataggtgcca ttcagggtag cttgaaaatt
    16681 ctctttggta tgttccgttg tcgtactcca gttagcggct ccacctacat tcgttgtctt
    16741 tgtaccagtt gataaatcaa gaatatccca cgtcatagtg gctcgattct tctgactatt
    16801 gcctacaatg agtggtgcaa tttttacttt tcgaatcaca tttaattgat tcatggtagt
    16861 tccgactcga actgaagtaa aggatagaat gggtttaaaa tattcaagta gagaaatctc
    16921 gacttccttt ctcggacttt gtcttcctct tgaatccgtc acaaaagcag agattttggc
    16981 acgaccaaca tggttaatac ctcctactcc gccattttgt gcagtagcta cattctggag
    17041 ttgtatccag ctatttcctt caaatttaaa cacttccgct cgataaccac tagctggaat
    17101 agttgatcca taaattcctg tagcaccatt aaaggtaacc ttgggatttg actccaatga
    17161 aacaaaggta ttgcctgtaa tggcattttg agcaatcgta tttaaatccg ataaactgat
    17221 agaggataga ctgggaacca ccgagctcgg aagttgaaga gtcacttgaa tccgagactc
    17281 accgatgacc tttcctccat aaatggtttg tactaaaatt gtgccaatac cagaggtcgc
    17341 attggggatt tgaggagcta attttgcaac ttctggtgtc caactacaag aagtggttgc
    17401 accagttgcg accactccac tcaaactacc aaattgccaa gtaaccttgt gtgtaaaatc
    17461 cccacttgct ctcgtaatag aaatggtaat tggagaaccc atcatattcc cagaaacact
    17521 agctgttgag gaccgtggaa tatctcgtaa tcgtaacgat tgataaccgg tattaagagt
    17581 tcctggtgac catccaccac ttcctgaaaa ggtcgcagaa aaaccaatgg tcttttgacc
    17641 attggaattg tgagcgatgg tgatggtttt atcaatgaga tgaagagaag agttcatgct
    17701 gtacatatct ggtctaccac tccaagaaag agtttgacca tctatggaaa cactggcact
    17761 acagttatac atcccaaagg ttgtttggcc atttcgtagc cacaattgaa cacgtacagt
    17821 tgaggtatta ttggcggtgc tagttcctgt ttcttctacc ctaagtagaa gattgtaccc
    17881 tcggtcatta ttactgccat aatctgccat agtctttcct ttctaagtct tgctgtcgat
    17941 gaagcgacaa accaaatgtt tggggttatg ccttgcagct tctaaacgat aatatccaac
    18001 ttgtagcgtt tcaacgaata caccatggtg gattttaatc acaccagaag taacggtcat
    18061 gaccgcattc cctgctgatt taatcatcat tccttgtggg gttaattcga taaactcaga
    18121 attatctttc ttaccaatga tgacaccatt atcacctgct cgcagataag tgttcacaaa
    18181 ttgtaaaagc aaggcattac ttttcaaatc agcttcaata gctgcgatgc gttgcgtgtt
    18241 tgtgacaaaa tcctgattga atttggtgag tgtttccttg ttactctttt caaattcctt
    18301 ataggtcttg agccaatcag aaacttcact ggccagtgcc ctcgcttcca tatccactcg
    18361 aatcgactcc gtcttctccg ttaggatatc caattgttgc ttcataaaac tttggtctgc
    18421 tttcccatca atttgcttca tcaaatctgc taaggatgga cctggagcgg ttgccacatt
    18481 gccatcctct aattgaacat tccgtaacca aaccacatct ccttgcgccc atgctcctga
    18541 ggataaggta aagacaaaag accatgttga attactggac accttccagc tagacaccac
    18601 tcgttcccaa ttagctgtca gtgaaacagg ctttgttcca cctaattcag agccaatcat
    18661 catggatact gctcttgaag cttttagttc caccgaaaaa gtcatggtct gtccaatgcg
    18721 tgtccgcaag tcaaaaaaat ctcgcttaaa gccaccactt cctgctttgg tacaagtcat
    18781 tttaatcgag atgccactga cagatgtact atcttctacg atttcttttc gccattctga
    18841 ctgaagatta gtgaaacttg tttctcgcat ggcatagtca tcaatgtaat tccgaccacc
    18901 taaaacagtc ccctcaaaga aagaagacca agtataatca cttgggttcg ttgagggtgt
    18961 tgcggttggt cgattcaccg ccagaccaaa atagcgtttt cccttgggtg agggtgaaat
    19021 gccttctcct ttttcactgt ccgcatacat cttccacgta tataaggttt gaccggtatc
    19081 tcccttattc ccatatacac caataaccac tggtgtcgtc gacgtttcat ttccatcagt
    19141 gaaaacagtt cgtttgtaat tccaaagata tttaatggta tccgttaaaa caggaatggt
    19201 ttttgaccaa ccactcgttg atgtgaggac tcctgttttt tgattactcg ctaaatagta
    19261 ttcttcgatt tgggaaatgc tgacaccttt gtctcctttt ggacctggag tgagtgacag
    19321 tttagaaagt tgactcttgg tagctagttc ttcgcctctc acttttaaga gtggtgtatc
    19381 aattgaaata ttcccattgg agtccaatga aaatactggc ttgtgacttc caggaataac
    19441 aaccttgttg gcattgactt ctatcgcatt taaatactca gtcaacacct gatgagaaac
    19501 caaacgacgc atccaagcag aattggacac ggttagttca tctatctttg ctttttgaat
    19561 agccgcaaag cgaatttctg ccgcctctga ggataaatag tcaactgctg cttgattgcc
    19621 actccgaatt gcttcagctt ttgctcgctc aatcccatct tctacatcct tgtttactcg
    19681 actcatattg aaatcaagta cacgactgag attggctcgc tccctttcaa tctgacgttg
    19741 aatctttcca tcattgagag ttaagagctc atttgcgaca gatgatattg gatttcctga
    19801 caccccttgt ccaccaaacg cgaccgtatc gtcaaatgag atggatgtga tttgctctgt
    19861 taaggcatta aagcgataac caactgctct tttgaccaca tcaacttgat gaagtctact
    19921 ttttagggtc acctcgtccc ccaactgcac ctcttgccca tctagttcaa aggcttcaat
    19981 ctcaatcact tctgaaacct gatctaaatg gtcattgcta aattttgcca tcgcccattg
    20041 tctcaattct tcctcggtat ggagattatt gttctcatac tccgcctcat gcacataagg
    20101 atattgatgg atgagaggac tctccaccat aaccgaaaga gttttctctt ccttttcacc
    20161 ttctggctta aaaattgagg acgcataaat gcgagtgaca atagactgac tagaacgagt
    20221 acgatcaaac ttttctaagt tatgatgcgt tgaaatgacc accccacgat tctttcctcg
    20281 atgttctttg atagtgaaca aaaagttatc acgaaccaac tctccctccc aagttcctaa
    20341 aatagaatgt ttcccatcca tcaacacttg gtaaagtgta gctgtctctg tcgtattaaa
    20401 cgaccgtttc tttgtaatat cactatcaaa agaaaagggt gctatatctc ttttagcttt
    20461 tgaaatcaat tgtccaaggg cactttggca ggtcacattt tgattcgaaa ctggtagaat
    20521 agagcgacgc atgatatcat ccgtaatatg ttggcaagtc acacgaaccc tatcgttttc
    20581 ttcaactggt ttcttaatcc gaaagagttg ttttttgaca tatggtactg gacatagtat
    20641 caatttatct gtcaccaact ctttatagat gcctgaatct gtgatgggat agtcaaattg
    20701 aagcgtaaaa tctccattca aagtttcttc aacttccgca ctcaaggttt catgaagagg
    20761 aaacccattc cattttggag ttttagtttg tccgtctaac aaaaataaca ttacgtccac
    20821 ccccataata tttcaatgtg aagactggtg attccagtac caagaacaac accaactttc
    20881 ttgtcctttt gattggcatc aagcgtgata aaatctcctg accaatgaac caactctcca
    20941 ctttccgtca aaaaactagg atgacttggg tcattatcca taaccaaagt tttgtttagt
    21001 tgctccaaac caataacttg ttcaccgatg gtgaaacttg tctctccagt cgatttacct
    21061 gaaacagtaa tcttaggata agacagagct gaaccaagca agggaatcat cccattcttc
    21121 gttaaagtat actcactcac tttcttaaaa tatcgagtgg ggtgacacag aaaagttacc
    21181 tccatgctga agactcctaa attatcttga acaatatcaa aactatccac cttatagcac
    21241 cagtaccgag tcattttcat gcgctcactt tcaagccaaa agccttcacg agttaaaagt
    21301 gcagaaaatt cattaatttg ttcctcagtc gcaccaataa ggtgaatccg ataagctttc
    21361 tcaatcgctt ttcggtgctt attggtcaaa accaaaccac cactcaaacc gtcatgttct
    21421 tgcagttggc ttttagaagt cggaatatga aaacttggtc tatcttccac gagtatcttg
    21481 taagggaatg aggccgttga tacaccgtct atgattaaag cattatgttg gatcacaaag
    21541 tcacccctct caatgattgt tgacgagtta actcgtcatg aattctttct gctacttgat
    21601 tggctagtcg tttgatatcg gcttcttctc ttatcactac atctgtaatg gaaacattga
    21661 tgaaattgcc accagttccc atggttgagg cgattccttc accgattgcc gcaagattct
    21721 gtttggttaa aggcaagaca gcctctttcc cagcttcacc accaaccatc agttgtttct
    21781 cattcatgcc aaaggctgtc ggtttcgtta gaatcccacc cttggcatac caagcaatcg
    21841 aaatcttagg caaaccgccc tttaaccaat caagtggatt ggcagaacca ctcacagaaa
    21901 agtgaggtaa aggaatatgt ggccatgaaa tcttaaagtt aaacagattc ttgatggcat
    21961 taatggcaga agatacaaca ttttttgcac cattgatggc gtttgagata gaattcttga
    22021 taccattcca gacacttgaa acagtggagc taataccact gagaatacta gaaatggtct
    22081 gttgaatgcc agaccagcca ctggatacaa aagaaacaat tgaagaaatc actgacgaaa
    22141 tgacggattt tatcgtgttc caaatagaag acacgatacc tgaaatggca gataggatat
    22201 tgctgatggt atttttgata ccgttccaag tgttggaaat aaactgacca attgctgaca
    22261 agatagtcgt cacaatttgt tgaatagctt gccaaactct ttgaagaaca cccttaatgg
    22321 tttcccatgc accagaccaa tcaccagtaa tagcctgcat aatggctttg atgattccta
    22381 atacaactgt gatagctgtt tctacaaccg ttttgataat ctcccatgcc gtggtgatga
    22441 ttaactgaat atttgcccaa gcagcttcaa gataagggcc aatcaaagac atgaccgtct
    22501 gaataactgt agaaatggca ttccagaccg tttccactgc tgcttgtata agggcttgat
    22561 tttctgtcca ccaagtggta agagttcccc aaatcgtcat gataaaggta gaaatctctt
    22621 gaatgatcac agaaataaaa gcatagatgg cattccagat ttcaatgact gcagttctaa
    22681 agccctcatt gttttgccac agttcttgaa tccaaacgac taatagggta atcactgcta
    22741 tgatgcctag aactgttcca acaatcggag ctgctgcaac gaccaatccg ccaatggtcg
    22801 ttcccatggc gactgctgcc gcttgaaggg ctaagagtcc aggtaacaac agaccgacaa
    22861 ctgtaattaa cccccccata atgacaataa aggtctgaat gggaccaggt aagttgctga
    22921 accaattggc gacagcttgt agtaattgag ccaataattc taagactggt gctagagctt
    22981 cagctaaaac accaccaacc tctgccatgg cagctttagc agtattttgt gcaatcgtaa
    23041 atttatcaat cggatcaagt gttgcttcat aagttgacgt cacaactcct aaagcattct
    23101 tagcagtccc tgataaatca ttaaatgaaa aagcaccacg cttaatggca tcgaccattc
    23161 gtggcgcagc ttttgaacca aaaacttctg aagcgagtgt cagactctct gtctcatttg
    23221 ttgaattctt aatttgttca acagtttttg ccagaccatc ttgtaaggtt aagccatcct
    23281 tggcataatt aacagccgct ttagataaag tagaaagtgc agcacttgaa tcgacaccag
    23341 ctttttcaaa gcgtcccatt aaggcgacac cctcgtcaaa ggataaccca agtgacttaa
    23401 tttgaggagc accagatacg accttactca ttagatcctg aacaccaacc ccagtttgtt
    23461 gggaagtaaa agtgaccgta tcaagtacca tggcgaggtc tttagactca agaccatagg
    23521 cttcaatggc ctgtttggca gaaatagccg attgcgtcac atccgaacca ttgatttcag
    23581 aatacttaat cagtaatgct gaagcctgtg ataaagcatc tcctgttaag ccaaactgag
    23641 tatttaattc cccaaccgca ctacctgctg tcgcaaagtc tgtaggaatg gaagtggcga
    23701 gttcagtcgc aatatcttgc atcccttcaa gtgcctttcc actagcacca gtcttggtga
    23761 tgatgatatc cattccttca tcaacttcac gaaaagcttc aagagattgt tgcccaaatt
    23821 cgattaactt ttgagagagt tcacccaatc ggtctccaaa ctccataagg atatctgctt
    23881 ttaataaacc attggtttgt tcaaggcttg ctgcagattg ttgggaggca ttggaaagcc
    23941 cctccatttc ctgttggaga ttgttatagg ctgtcttagt atcgttgagt gttttctcaa
    24001 gccgattggc ttcgacagaa ttctcgccat attctgattt ggttagttct aactgacgtt
    24061 ctaaattcgc aatttgacgc tcaacgagtt cagactgact agcaattctt ctttgtgcta
    24121 aggctaattt ttcagactct gaagcatttg aaccaagctg actctcttgc aaacgaaagg
    24181 tggaattgag tttctcactc tctgatgcta attgggcctg ttcagcttct aaactgttta
    24241 atttcgaccg gttgctttca actgtgtttc cgtgagaatc aagcgctcga ttaactcctt
    24301 caagttttgt ttcatagctt tttaggacat tttgtgttgt ctcaacttca cgttggaatg
    24361 ctcgatattg ttctgcacca atatccccac gttggaattg cgcttcaact tgactttgtg
    24421 cctgacgtaa ggtttctaat ttttctttcg tggtcgcaat ctgttttgaa agcacctctt
    24481 gcttttgagt aaggagagtg acattgccag tatcaaattt cagtgcttta tcaatttgtt
    24541 tcagctcttt agtggattcc gctgattctt tatttattcc cttcaaggct cgttgtaaag
    24601 gttgggtgtc accaccaatc tcaatcgtaa tgcctttgat gttaccagcc atagttctcc
    24661 ctcctttctc taaaagttat caaaatcagc ttgggttgct cgtctagttt gtggtttgtc
    24721 agaacgcaag gcgacataat ccgtctgata atccagtgcc atcccaatag agatgtgttt
    24781 taaatcatcc atagatagtc cagtttcctt gcaacaggac aagtagcttt ctaccgtgaa
    24841 gatttcttca ctcgcttcat cggtttcatc gacttttttc gagtcgacat tccttgattt
    24901 aacatctcca tcaagactgg accaatctcc tgaatcggta actcctccat ctccataaag
    24961 aaaacatcaa atgctttcac ccgtggattg gctgatttgg caaataccca aaatagtcga
    25021 tgaaagaagg tcatatcgaa atcagaaaga atggagacat ccatattttg tgcggttagc
    25081 tcctctcctt cttctaactg attaagttgg tctaaaatag acttggcatt aatcatttga
    25141 aagaggtctt gaaagtaatc tttaccgaat tgttctttat aggcaatcgg cgtgtaagca
    25201 ttacttgcta gtgggtaaac tttcccacaa atcgtcacct gttttctcat gtacgactac
    25261 ctccacttgc gacagttggc tcatatacag acttaaacca atttttcttc actgtttcgg
    25321 gggtctcttc tgttgtccga cgacgaacca cctgatcaag tggacgtgga ctggctgtga
    25381 atttcaattc cacttcattg atatcactac cattcttggt ttttgagcct actgttggtc
    25441 gagaggcata acagtaatag aggacatgaa gtgtctcctt cttatcccct tcaaaacgga
    25501 acattaatgc gaagtttttc cgagttgcat tggatgattc cgtaatcacc ttattggtat
    25561 catctagtgt ttctcctaac actcgagtta agaactcttg tgttaaaagg gcaaccttca
    25621 aagttccact ataaccatca ttagactcag tcgtataaaa gttgatatta tccgctttgt
    25681 aggaacctga atctcctgtt gcttcaaggg tcaactctgc agcacctctt aatcgttcaa
    25741 cattcccata ggttaaggct ccgtcactcc cttccgttgt gacttctgcc cagtggacat
    25801 cttgtagacc aaacgtgact ttattttttt cagccatatg ttctccttct taaaatgcaa
    25861 aatgaaaagc aacctgataa agttgctcgg tatcaatata agtttcaacc ttgtcaaaaa
    25921 acaaatgttg ttccgtcaga aaagtttcaa tcttcccttc aacgtcgagg tctttcttcc
    25981 ttgtgtaaag ttcaagaatg aattgcgcgc ccttatggta aacctgattg tctgccccaa
    26041 agttttctga ttgcggacta taataaacca taaaaggcgg acttggactg tgaccttctt
    26101 cgaagtgatg ataagcaatc ggaagatgta aggcttttag ctgtttaaaa aactcctttt
    26161 cactcataag ttacctcgaa tcttcttctc caaagcttga actgcttttt gttcaacggg
    26221 gccaatatga ggacgcccgg aaactcgacc accattttgt ttggcatagc cattctcaag
    26281 tagatgagtt aggcctggtg tccgattatg aatcgttttc gttttaccag tggacgtgct
    26341 actcgtttcc tttgaagtcc accctctcac atagcgacca ctccgcttgg gcgatgtaga
    26401 ttttagagtc gcaatcgcct cgtccgttac atcatcgact gcttcaacta ccgtctctgt
    26461 tgtttgatgc aagtattcct ctagctcctt ttcaatagcc ctactcagtt catcagctct
    26521 catggctctt cctccactcg acatttgacc attttctttt caaaaaagac atggtcaatt
    26581 gaaagaatat taaacgattt cccttgaaag atgagttgtg tattggtggt atcaagtgag
    26641 gcgacttctg aatcatatct aagagtgact atcagctgaa gttttcgttt cgtttgaccc
    26701 acttcaaata tctcatcact atttgcttga ttcacactag cccatcgtga aaataaatta
    26761 gtaaactgac ttgtttcgtt accaatatca tcttgagtaa tcacacgttt cttgaaaaga
    26821 actcgctggt ttaaaggtgc aattttcatt taaaacacct cttttcgcaa accacctaac
    26881 agatgataga gcgtctcttt caaatcatga tggttagcct cttctcgatg ctcgaaaaga
    26941 taagctaccg catagagcat tgcggagcga ataagcggtg tatccaccaa ctcatccact
    27001 cgcaatacgt ctttgcagag ttcttcactc gttgcaatca agacttttat caaattatct
    27061 tcctcagaag tatcaactcg tagatagatt ttagcttcta aaagcgtaag catatcttac
    27121 cctttcatgg tgagcacttt gacagcttca ggaagaacca atttcccatc cacacgttgg
    27181 ctacccaaga atccaacttg accactagtc gcatacaact cgttcagacg tttgaaggaa
    27241 cgcccttgac ggtcactaat ccaatagtgg gagaaatccc caaaagcaag aactttttgc
    27301 ccagaagcaa tttctggggc atagcttgac gtatagtatg gtcgattcaa aatcatatct
    27361 ggtactcctg cttgaacaga tggttgccag atgtaatttc cgttgttgtc cttcaactta
    27421 cgcagagcct tgatagtaga atcattgaga atccaaacgg aattcttacg gtaaggtgac
    27481 ttgagagaat gataaaggtc catcacatca tcaaaggtga tgttggcacc tgttgaagta
    27541 accccttcag taactgtggt aaagacacca gttggtttac cagaaccatc accaatcaag
    27601 aaggcttctt cttccttaga cccaatgcgt cgagcaaact cttctgccat ataggattca
    27661 aggtcaaaga cagagtcatg aatcaactcc tctgaaatct tgatagctgt cccaaccttg
    27721 taagcaccaa gagtcacttg gccaaaggtt tcttcactct ctgggtaagt accattttca
    27781 tccatccacg cagccgtacc atgacctgtc accactggaa tcttgcgctc accatttgag
    27841 gtcttgataa tggttgccaa acgacggaag aagttctcct cttcaagagc cttaatcaag
    27901 cggttctcgt attcatcagg taccaagtaa ccaccttgac catcattccc aatgttcaag
    27961 gtattattaa cgtcaaagaa attcttttga cgcacatttt tccagaatga ttggttgtaa
    28021 acagttgaag ccgtacctgt cttctcatca ccaccagcat cagttcgtgg ttgagagaca
    28081 atagccgcgc tagttgcttg tgacagttca cgctcaatac gttcttggcg ttccaaacgg
    28141 tcaatctctt ttccaaggtc aaccacttct tgttccaact tatcgtagtg ttcactatct
    28201 tcctgtgaaa tcatgccctt ttcatcacga catgaatcta agaagttctt ggctttatcc
    28261 caaaccttgg cacgtttttc tcgtagttct aaaattttgt ccatagtata aatgtcctcc
    28321 tagggtttta ataactgtag tcgtttttcc aaggtgctta ctgcaacctt gggtttatct
    28381 atcaactgtt cacacttggc gaccacttga ttggttactg tctgcattga aaatgcatag
    28441 gcatcttgtg actcatcatt gtcatgagtg ccatcgaaga taatctgatc cgcaaaacct
    28501 aactcaactg ctttctttgc attgagccaa gtctctccat ccatgaggtt tgagagtttc
    28561 tgacgactta gagatgtctt aatctcataa gcgttgataa tcgactcctt cacttcattt
    28621 agaagctgaa tagctttttc catctcacgt ttgtcaccac tcgcaaaagt ggccggatta
    28681 tgaatcatca tcatagagac tggactcatg tttacaattg ttccagccat ggcaatgaca
    28741 gatgctgcac tagccgcaat gccatcaatg tttacggtga ctgctccttt ataatccatt
    28801 aacatgttat aaatctgagc cgccgcaaag acatctccac ccgggctatt aatccaaagt
    28861 gtgatatctc cttgttctga aagcagttca tctttaaaga gttgaggagt tacctcatcc
    28921 ccaaaccaag attcactggc aatcacacca tttaaaaaga gggttcgttc cccctcattt
    28981 ttggcccaat tccaaaattt atgcatcttt gataaaacct cctgcgtctt ctaacttggt
    29041 catgttgcca ttgaccaagt aaaggtcacc accttcttct tttgaaagtg ggttcatgtc
    29101 ctctaagcgt ctaatatcat ttgttgacaa ccaaccattt tgacggccta tggcatagcc
    29161 attcatccga ctctggtagt cccctcttaa caaaccgtcc acattgaact tgataaagta
    29221 ggtcttcttc tcatcaggta aaagcagagc tttttgaaag gcttgttcaa atcgaactac
    29281 ccaaggatct agcgtgtact tcacaaactc caaggactgt tgttcaatat ttgaaaagga
    29341 agatttctcc aagtcaccga ccatatgagg tggtatccga tagagtcttg caatttcatt
    29401 gatttgaaac ttacgtgtct ctaagaactg ggcttcattg ggtggaatcc caatagtctt
    29461 atagatcatg ccttcttcaa ggacagcaac cttatggcta ttgtttgtgc cttgatagac
    29521 ttgattccac gactctctaa ccttactggg gtctttgaga ataccgggat gttccaaaac
    29581 acctccggga ttcgcaccgt tcgcaaagaa tgatgcacca tactcttcag tcgccaaggt
    29641 cataccaacg gcattttttg ccatagcaat cggtgagtag ccaattaaac catcaaagcc
    29701 aaggccagga acatgtaaaa cctcactttt tttgagaatg atatttcctt tatctttgaa
    29761 gttcgggttg tcctcttcac tacgttgata aacatagtag agttcaccct tatcatcacg
    29821 cttgacggtc atcctatctg gcaacaaggg atacaaggct aagacctgcc ctcttccatc
    29881 acgaataatc tgggcataag cattgcccca aatcaataga tgactcatga gtgtctcacg
    29941 aaagacaaag gatgtcatgt ctggatttgg ttcatcatga agtaggtaat agagctgatg
    30001 attaacatct tgttccttgc cttcatccgt atgcttgtaa acatgaatgg ggagactggc
    30061 aatcgcctca gctaaaattc gcacacaggc atagactgca gtggtttgaa gagctgtcat
    30121 ttcattgaca ttcttccctg atgtggttcg tccaaagagg tacgaaaaat ccgacccctc
    30181 atacgagttg gtcggtttgt ctcttgccct ctttaagcct atcaattcta agattcccat
    30241 agagcctcct tctaaaaact caaaattcct cgctggtcgt aaatactacc atcatcacct
    30301 ttatgacgga tacaacggtc tagtcccata atgagagcta caatcccatc aatcttatcg
    30361 actgacttct ctttgtctgg cttgatgtta ccagctggat cttgcctcat gaccacattt
    30421 tgagccatcc atttcaaaac tggatgacca gcgtgtgtga ccctgccctc catcatgagt
    30481 ttgtataatt ccttagatgg tggtgacata tctttatatc cctgaccaaa tggcactaca
    30541 gttaagccca tatcctccaa gttttgaacc atctgtgtcg cattccaacg gtcgtaagca
    30601 atttctttga tgttaaactt agtagataag tcttcgataa aacgctcaat aaagccataa
    30661 tgtaccacat tcccttcggt tgttttgaga tagccttgac gttcccaaac atcataaaga
    30721 acatggtcac gtcgacatct caaaggtagc gtgtcttctg gcaaccaaaa gtagggcaac
    30781 acaatgtaag attcatcctc agtccgtggt ggaaacacta aaacaaaggc agtaatgtca
    30841 gacgtacttg ataagtcaag ccctgcataa cactctcgac caagcagact actttcaact
    30901 atcggtctat ttcctttgtc atagacatgt tccgcaatcc acgtcaccgt ggaacttgtc
    30961 cacatattca aacggagttg cttaaaaaca ttttcttcag ctggattgtc gatggcattt
    31021 cgataggctt cacgcacacg gtcaatctga atggtgtgac ccaatgatgg atttgcctta
    31081 taccaatttt cttcatcctg ccaatcctca tcattagcta ggccataaac aactgggtag
    31141 aacgtttcat ctttctttcg tccagacaag atatctagtg ccttcgtatg aagttcataa
    31201 cagatagagt tcttatctgt acctgcggta gtgatgataa aaaatagtgg ttgttcacgc
    31261 gcatcaccag aacctttggt aagaacatca tagagatggc ggttgggttg agcatggatt
    31321 tcatcaaaga caagacctga cacattaagt ccgtgctttg tcccagtctc cgcagatagc
    31381 acttgataaa aacctgcatt attgtaatta acaatgcgtt tggttgctcc catgattttg
    31441 gatcgcttgt ttaatgggtt tgacatccct accatttgct tggccacatc aaatacaatg
    31501 gaggcttggt ttctatcaca agcagcaccg taaacttcag cacttgcttc accatccgca
    31561 tataacagat agagggcaat ggcagccgca agctcactct taccattttt ctttggaatc
    31621 tctacataag cggagagaaa ctgtctgttt ccatcctctt tcacaattcc aaataagtca
    31681 cgaataatct gttcctgcca tggtaaaagt aaaaagtgct tacctgccca cttccctttg
    31741 gtgtgtttaa gattttggat aaaggctacc gccctatccg ctttcttctc atcgtagtgt
    31801 gaggtaggca acataaaagg acttggttta tagtgataag tcatcctata cctccctcaa
    31861 tagactttcc atttcatctt gttctccatc actactatca gcctgtattc gacttcttgc
    31921 agaaggggtt agaccaaact gctcggagaa cttgagcatg attttcatgt tggtttgagc
    31981 aatggaaact tgtggaacct gctggaggta gccgttcggt gttttgataa tagagccatg
    32041 tttcgttagg aactcttctg cctctttcca tctagcgtac gcttgacagt atcctgcaaa
    32101 agccgtcata tccatatctg tcaaaagacc catggactcg agcaccttgc tcatgcgttt
    32161 ccattctttc ttggcatcct cttctaacca cgatgggcat cgaggagctt tcttagttgg
    32221 tttaggttct ttatggttga gaggacgctt acctaagtta ccttcaagaa cttttaaacc
    32281 agttggtttt ggtttacgtc cacgctgtgc caggtcgctc acctcctatc ttccattttt
    32341 ctgacacgca aaaaggcctc ttgcgagacc ctttcggttt attgtaattg ttcaaaagcc
    32401 cattttactg catgcccaaa tcttgaaatg tttcttcagc ttcaatgatt ctgtcaaact
    32461 ttctttcaaa gttatcaaag tcttctaggc tatctatggt ttcatagata tgaagtccaa
    32521 cccctttgta tccattgaaa gctacaatca cccaatctat gtaagggatg atacttgagg
    32581 ttgttccata gtttgaaaca ttttttcaag tgttgtggat gttgacattt tttgttctcc
    32641 tcttcttttg ttgtgaacat attacctcta aaggagttta atagccagtt ataagctcac
    32701 tattatcaat tataatctgc tattatttct ttaaaaactt ccctatccaa agcattcatt
    32761 gcatctaatg agaccccacg atggtaagca tacacaagat gaccgtcacg tttcaaggtc
    32821 aaatgagcaa tctattcacc attgatataa cttgggtcag ttgaagattc tctgagcaaa
    32881 tcgcagacat aatgtctctc acctctatca atgactacca actcccacat agccttattc
    32941 cacctttact atcctatcaa ctccatagac gacactcagc ccactaccat tatcccaatg
    33001 aactaaaact gaagctaagt cgtcatgtcc atagacagta cctagcgttc caataggtgg
    33061 tggaaatgga tcatccattt caagtagttt aactcgacag cctacagggt ataaacattc
    33121 aactctttct tttgcaccgt tattcattgt gaagcctcct tctttttggt agctgtatat
    33181 taccattagt accaaacact atccagtaat catctaagat ttaagcaaga aagaatagcc
    33241 tttccaatcg cataaacaac ggtaactgtt acaccattac ctgcttgttt gtagagttgg
    33301 gcatcagagt ttacggcttg agctttctca aagaggtcat ccgtgaaacc ttgaagtcta
    33361 aaacactcac gaggtgttaa tcgtctgatt ttaaccattc gaccattcca gaccaccaca
    33421 cccatttgac cactgcaaga gaggttatga gcaatgccct tacctactct tgctcgtttc
    33481 gttggagatg ctggataaga taaatcaaca gagtcaccaa gttctgccac ttgataccct
    33541 tgctttgtac cgttccttac tttaattcct tctacaactc cgtgtctgtc ttgagaagtt
    33601 aaggtaaaca taggctcacc tttctctttt aatcgacgac cattttgacg tttgttcact
    33661 cggtctggag ttagaatagg ttgtacttcc atcacaccag agttcatggc tgtcctcttg
    33721 gtagaacctg ctgtgtaacg agcagtgatg caccgtgctt ggtctgttac ctttggtttg
    33781 gttgtagatt ggtcaatcaa gtaaagccct gtctttgccc caacaccgcc accagtgcca
    33841 actaaagttg tagaaatacc gtccgtatca tagacacgat aggactgcat acctcctaca
    33901 agttgcttaa gattgccact gctttctggc tggagaggta atacttgtcg tctacctctg
    33961 cttctaagat gtccgatagt gtagacacgt tctcggtttt ggggaactcc gtaatctttg
    34021 gaattgaaca cttgccattc aaggtcgtac cctgcttcgt ccagttcaag gagatagtcg
    34081 aggaaatcga ctcctctgcg acttgataaa agtcccttaa cattctcaag gatgagccac
    34141 tcgggcttat cttcttcact ttggctcttg aggaggtcaa cgagtgtaaa aaagagtcca
    34201 cttctttcag ctcgtagtcc agctcgcttt cctgcgatag acacattttg gcaaggactt
    34261 cccgcagtcc atatatctgc ttttggaatg tcatttggct taagttttgt aatatcatct
    34321 gaataccatt ctccttccgt atcatacatg gcttggtaac ttctaaccgc aaatttatcc
    34381 ttttcacaat aaccaatgca ggtcatccca gccagctcaa gtccacgtct aaaaccacca
    34441 atacctgcga agaaatcaat gaaggttaac tgattcatac tgacacctca actaaatccg
    34501 catacttgat ttctttccca tctcgcataa ccttgattga ttggtcacca gtcgcttcaa
    34561 agtaacgttt cacaatcacg tccacaaact tctcatccaa ttcaatacca taacaaatcc
    34621 gacctgtttc ttcacaagcg atcaaggttg acccacttcc aaggaatgga tcaaggacga
    34681 ctgttccacg catggatgag ttttgaatgg gataggccat caattgaatt ggtttcatgg
    34741 ttgggtgctc tttgcttaac ttaggacggt cgtattccca aatggtagtt tgtttgcggt
    34801 cagaaaacca ttggtgtttc cctttgttct tccaaccaaa gagacaaggc tcatgttgcc
    34861 actgataagg gctgcgacca agaacaagtg agttcttttt ccagatgcag catccactca
    34921 agtaaaagcc tgcatctttg aacgctttac ggaagttata cccttccgta tcagcatgaa
    34981 aaacatagat agaggcatcg gactccatgt tctgttcaac attaacgaac atagcaaata
    35041 agaacttata gaagtcagag tcactcatat tgtcgttctt aattttacct gctgtctctt
    35101 ccacattgac gttataaggt gggtctgtca aaactaagtt agctttctta tcccccatca
    35161 atgtgatgta agtttctggc aaggtagagt cgccacaaat gacttgatgt tttcctaagt
    35221 accaaatgtc accttgttta gcaacagttg gtttcttgag ttcttcatcc acatcaaagt
    35281 catcctcatt gacttccttg ttatggacct tagaaaaaag ctggtcaatt tctgggaatt
    35341 caaaaccagt taggtctgta tcaaagtcag cttcttgtaa gtccaacatc aaatccgcaa
    35401 gcaactcttc attccagcta ccagtgattt tatttagggc aacgttcagt gccttaacct
    35461 tattctcatc ttcaatatgg acttgaacgc actgtacttc tttaaagccc aagtccttca
    35521 agaccgtcag tcgttgatga ccaccaatga tagtcatatc atagttgaca ataataggtt
    35581 cgacataacc aaactcttga atcgaccgtt tgattttttc gtattccttg tcgccctttt
    35641 tgagagcctt ccttgggtta taactagctg aattaagttg gtcaactgtt aatgttaacc
    35701 atgtcatact ttctttcgtc atcatgtcct ccgtggtgtt cttgcgcagg ttctgctaca
    35761 aaattttctt ctagggctac cataagctgt gaaggtctta ccgcacagtt cacaaacatg
    35821 gctatccgct ttttcaggat gttgcgtcac ccaattctta cgacggcaac tgtctgagca
    35881 gaattgtttc gttctccctt tacgaggttg ctcaatgggt ttcaaacatt ctttgcacaa
    35941 ttgtccattt ctttgtccgt cagaaatcat agccactact gcctgaccat aaccctttag
    36001 atgagggtta gtactgcagt agcgtttgac tgccgtcaga ctgaggtcaa gcaagtttgc
    36061 gattttgcga tagcccagac cctcgtttct cattttccac acttgttttg ctttaaaatc
    36121 gttcatttta acctctttta gttagctttt atcccagttt ttgggtaaaa ataaacgaca
    36181 agatttaatg aaaaaccttg tcgtagataa tgtttggggt cagtaaaaat ctatttttgt
    36241 gtaccccctt ttgaattttg cgatttttca cgtttgaggg ggcgtcggtc ttagtctccc
    36301 aagggtttag agatttcatc ccccctcccc caaagggtta aaaattagat acttttgtaa
    36361 cgaaactcca agactaaaat cgatacgtat actccacata tcggtcagtc gtcttggtct
    36421 tcctatcatg acaagactta caaagtgctt gccagttggt ttgattccaa aagaggtctt
    36481 ggtcacctcg gtggggtgtg atatggtcaa ccactgttgc cttggtcaat cgacctttga
    36541 ctttgcattg aacacaaagt ggttgaactt ttaagtaacg aagtcgtgct ttattccatt
    36601 gggcattgta tcctttggct ttggttgact tggtatccag ttggtagttt gctttatggt
    36661 tgtcacaata cttgtgacca taaggtacta ggttaggaca accattttgt ttacaaggtg
    36721 tgcttggtct tcttggcatc ttacttctcc caaggaagat aggcttttgt gaaatgccca
    36781 aagcaagatg ttttggtgta gtctacattc aagagatgaa gttccttaat gataccttgt
    36841 ggggtcaagt cgtagcgttc acgaatcatt ccttccagtt gtgttgaggt gtagcgactg
    36901 gttccaaagg tttctacata taccgacaca ggttctgcaa ctccaatggc ataggctaat
    36961 tgtacttcac atcgtttcgc atacccttct cgaacaatat ccttagcaat ctttcgtgcc
    37021 atgtatgctg ctgagcggtc aaccttgctt gggtcttttc cagagaaagc accgccacca
    37081 tgatgtgcaa aaccaccata ggtatcggcc acaatttttc gaccagtaac tcctgcatcc
    37141 gcaaacgaac caccaagaac aaaacgtccg gtcggattga ctagaacttt gaaatctaga
    37201 ttctgacggt aacgaagtgc tactgacatc atagcttcag tcacaattcg tttcactttg
    37261 gcaaggttgg ctatttcagt atgttggatg gaaactaaaa aggtatcaat ccgtttcttc
    37321 tcatagtcgt aggatacctg tgctttcgca tcctttccaa gtaaggggtg accaagcgac
    37381 atcagtttct caaggactcg ggttgctaga acataaggaa gtggcaggta ctcaggtgtt
    37441 tcgtctgtcg cataaccaaa catcattcct tgatcaccag cacctccatt atctactcct
    37501 tgtgcaatat ctgagctttg gagtccaagt aggttagtta cccgaacatt tttcatacca
    37561 agtggctcga caacctgacg aacaatgttc tcgagattaa agtaatgtct agttgaaatt
    37621 tcaccagcta ccacaacttg gttatccttg attaaagtct caactgccac tcgacttgat
    37681 ttgtcatact ttagacactc cgtcaaaatg gcatctgaaa tctgatcaca gattttatct
    37741 gggtgtccaa ctgaaacttg ttcactagaa ataatcataa ttcctccacg caaaaagccc
    37801 aacctttatg aggctaggct ttactttatt ttactgattt ttggcctgct tcgtaggctc
    37861 tctccaatgc tcttttgata ccccataccg aaacatcgta gaagtcaaga ttgtcgctcc
    37921 aacgtttttc caaggtttct acaaaaagtt cttcttttgc aatttctgtt aaaagggcat
    37981 tgagtttttc ttgttgacgc tttgtcatgg tattgttctc ctcttctttt gttgtgtaca
    38041 tattaactct aaaaaggaga tatatccagt catttctgca taaatttaaa gatattttaa
    38101 acagttaaaa ccatcaaaac cgcctcaaga atggcttcac gttcttctgt ttcaggatag
    38161 agttcccatc ctctgtcgta agaaacgatt gtttcttcag ctacttcaat atctaatttg
    38221 aaaatacatc caaggtcaat tccaacttct gaaggttggg gactaacctt ggcaatgtaa
    38281 tccacttttt gtccctgata atcaatccgt ccgtcagtcc acatggttat tctcctttgg
    38341 tttttctagg tttatattac cctatcagcc aaagaatatc cagtcatttg agggagattt
    38401 ttatcttttt tgacatctta agtatatcac atgtctaggt tgaaaatcag tactaaacca
    38461 gtacaaattt agtgctaatt tagtaccaat ttagtgcact caaccatcac tagataggga
    38521 tgtgataatc ttccacctta agttcaagac tttttgaagt ccaacgataa cacttgcgta
    38581 attgttttag aatcttacgc ctacgataag caagtgttga atgactgatt tcataaatct
    38641 cttctagttc agtccaactc ttgcctaagt aaattaaatc cttggataat ggcttcaaat
    38701 cctcaggtat tagctccata acgaactcaa agtagtaaag atccattttt aagcagtagt
    38761 aggtattaag caaactactg aggtactctt gattttcttg ttcttgtttt tctctaaaac
    38821 taagagaaat cagctcacta cgaccatggt ttttactttt cttgaccttt tcatgctctg
    38881 acttctcaaa taccagtgac tgaatcacac tattctctga gataggtcga tagttgagca
    38941 atttctcctg aactaaatgt aacttcattt tcatgtcacg atagttttta gctatatatt
    39001 ccaccttatc catctgttcc tcctacttgt gctttaacag cttcaatcag ccgttcttgt
    39061 tgtgcatctt tgtttcctag tgccttgagg atttcctcat caatcgttcc ttcagtcaca
    39121 atgtgttgaa taacaactgt ctcagcctcc tggccttgac gccaaagtcg tgcattggtt
    39181 tgttggtata attccaaaga ccacgttaat ccaaaccaga ccaagtggtg accacctttt
    39241 tggaggttca acccatgacc tgctccagct ggatgaatta agccaactgg tacattgccc
    39301 ttattccatt cacgaatatc ttcttctgtt ttcaacaccc gactcgttac cttgagtttt
    39361 tctaaacgac ccgtaatccg agccaagtca tgtttgaacc aataggcaac taagacaggt
    39421 tctccatttg cggattcgag gatatcttca agggcatcta gtttttgttc atgaagtggc
    39481 acaaccgtat gatcatcaga atatacagct ccattggata actgaactaa cttgttcatt
    39541 aggcttgcag catttgcggc agttacttct aatccgtcta actctgacaa gacatactct
    39601 ttcttaaact gaatgtattt ttctttttcc ttttctgaca tacgcaccag tttcttagtt
    39661 gaaattaatt cgggcatatc cagataatct agggctttca tagaaatggt aatatcacta
    39721 atcttgtctt gaatttgaca ctccgcataa tccatgggga tgtattcata gacaatgttt
    39781 ccattgcgac gaccttcttc aaagtagcga ctacgaaact caccgatgaa gcgaccaaga
    39841 cgttctcctc cgtcaatgac tttaaactct gcgaacaagt ccatcagtcc attcgagctt
    39901 ggtgttcctg ttagtccaac cactcgcttc atgtaaggtc gcatagtcat gaaggctttg
    39961 aaacgcttgg actgccatga cttgaaagaa ctcaattcat caattacaac catgtcccac
    40021 ttgaaatacg gactgcattg ttccaccagc caaggtaggt tttcacgatt tacgatatag
    40081 atatccgcat ctttctgaag ggctactttt cgttgcttgg gagtaccaac tattttcgaa
    40141 taccgtaagt tacgcaactc agaccattgc tcaatctcgt cactccagac agtatttgcg
    40201 actcgcagtg gggcaataac caaaacctta gtaacctcaa atcggtcaaa catcagctca
    40261 tttactgcag acaaggttgt agccgtcttc cccatcccca tgtctaggat gactgctgca
    40321 taagggtgac ctatgatgaa gtccttagcg actacctgat agttatgtaa tgtcaatttc
    40381 atctaacact tctccaatca tctcaatgcg gtcaatgaca taaaccttaa agcctaaccg
    40441 ctcgaacagt ttatgtcttg acacttgtaa cttccttggc ttctggtcgg gagcctttac
    40501 ttccaccaag ccaaacttgc ccttgggtaa aaacaccaaa cgatctggaa caccagaaaa
    40561 agagggcgat acccatttag gacaaatacc tcctcgagct ttcacagact tcaccaaagc
    40621 ttgctcaacg tacttttctc tcatcgttct aaatcctttc gtcaaattga agtgtgtagg
    40681 tctagtgcag tcatttccaa aactcctctt ataggctttt tttatagtaa tttttgctta
    40741 taggatagtt ttagaaaaga ccataataga cctacacaaa atcaaaaaat gtcactcatg
    40801 ctggtcgttt taatcatcta tctgaaacct cattcaaaat aagttccaac ctttcagtca
    40861 acgacttaca cacctaaaat aatcaaatac ctctattgtg gagatctgtt gactaaaaat
    40921 gctggtcatt aatctaagaa atcataacca tcctctacca atttcaaacc aagaatgtgg
    40981 ttccctttac ttgtccgttt tcgtttaaac cctgcctgct caagtgcaga ataaaaatcg
    41041 gttgtactgc gtgtatactc catgtttttg gcgcaatagg cacgatactg actgtatagt
    41101 tctcctgatt tttctgacaa ctggtcgcca acttgacaac agtcaccaag gaagtgtcct
    41161 aaccaatcat ttgcttcacg ataggctttg acggaagatg aaacggcagt tggtacattt
    41221 gttttgaagt tcgctttgat ggctttttct gcaccttcga taatccaaga catgatggct
    41281 ggtgctgcat tgtcatacaa atggtccgca aagtttttga tgtcagagcg accagttatt
    41341 ttggcattaa agggaataac aaccaaacgt cgccaagttc catcatcgtt cgcccctact
    41401 ttaggcagat ggttggtgta aagaactagc gtgtgtgagg gcacgaagtg gaatggatcc
    41461 ttgtacttct tctctgcttg aatctcatca gttgaagtaa tctgcttcac aacggctgta
    41521 ttgagtcgca taccctcagc catctcagaa gcaatgacca gtcgtttccc tttaagctca
    41581 gcaagctcag gactgacatt tcgcttgttt gacatagtta aggcatccgc agataattta
    41641 cctgaatagc tacctagcac acgagcaatg gtattccaaa aagtagactt gccgttcgct
    41701 ccgcctccgt aggcaataat catgtgttcc tgatagacct taccgatagc tgccatacca
    41761 ataatttctt gaacataatc aattaattct tggtcattac agaaaaaggt agctaaagtt
    41821 tcctgccata atcccattcc ctgatcacta ggggagactg cggtaatttt tgttatgtaa
    41881 tcttcaggat tgtgttcttg ttgcccattt attccctttc gtaaatcata ggtagcctct
    41941 ggggtattga gtaataagtc atcactatct aattctgaca attctactga aagaattggt
    42001 ttagctgtat tatagacagc catcaaattc ttatagtcac gatgtttcat aacaaattta
    42061 tgaaactctt tagctgcaag ataagctttt aaatatttca attgaagtgg agtttcgact
    42121 gcattttcta gacgctttcc tccagccttt atggttaatt catcaatacc tgaagactga
    42181 agttgcttat ccgcagattc caagagtgca atcgcttcag caagttgctc atcagtaaag
    42241 tgtacaactg cacctagtgc caactgctta ttctcacgcc agtgagttcc gtcatagtaa
    42301 agatagtcag ttgcgttggt gtaggccagc ttattagcat attctctagc aagcacacca
    42361 gcctcgccta catctgagta atcatctggt tttaaagttt ctcgattgaa agcatcggga
    42421 gccacataac tttttgatgt ttttatagtt ctgttgtaga atcggaccgc gctcccccaa
    42481 atggtatcta actcagcttt gtcaagtggc ggtacacatt tctgtgcctg ctcatcaaaa
    42541 ccatccctag cttcttgggt cacgcctaac cgtttgagaa ttttcgcagc aaaaactgac
    42601 atcgttgaat tacgactccc ttgctggatt ggtccacttg gaggagtata gaagtctgca
    42661 tcgaaatctt cctcatcttc aatagaaaca acttgaaaca aatcttcatc aatagttagc
    42721 catgaatcat gccatataac ctgtgcattt ggatttccaa agaagaatcg tgccgcatcc
    42781 ttggcatgat catcaaaaaa cttgtattga ttacagagtt cttccttcat tgctacatag
    42841 atgtctttat ctgttacctc attaatttgg aagtagatat gaaacttggg tctaggtgct
    42901 tttcctgcct tagtctgcat gtgactacgg ctcgtcacca aagcgaagtt ataatccgca
    42961 aagacgtctt taaggaattc ttcggtaagc cattcatcag gattttctgt gtgatcatta
    43021 tcaatatcca tgactaacac atctgacttg ataaagttgg cattggagcg agtattttgg
    43081 acaaaaagac ctgccacatg gtcgaattga gctacgtttt gtaagatagc tttatcagtg
    43141 atagttacca tatttggata gaccgtagtt gtttgtattc ctgtctgtcc agaatgagat
    43201 agggtaaatt tcatttgata tgcctccgtc tttagttttt aaattaggaa acttgcttcc
    43261 taccttacta agtaagaaac agagatgatt ttccgcactt ccgaaatttt ttttagaaaa
    43321 gtaaaagttt cct
//
LOCUS       DAUELZ010000005        38233 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.005, whole genome shotgun sequence.
ACCESSION   DAUELZ010000005 DAUELZ010000000
VERSION     DAUELZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38233
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.005"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(23..757)
                     /locus_tag="VHI09_000170"
     CDS             complement(23..757)
                     /locus_tag="VHI09_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120080.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVMFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
     gene            complement(757..1443)
                     /locus_tag="VHI09_000171"
     CDS             complement(757..1443)
                     /locus_tag="VHI09_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ6120081.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMAQGGNH"
     gene            complement(1570..1800)
                     /locus_tag="VHI09_000172"
     CDS             complement(1570..1800)
                     /locus_tag="VHI09_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEQ6120082.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            2097..4379
                     /locus_tag="VHI09_000173"
     CDS             2097..4379
                     /locus_tag="VHI09_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ6120083.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRIIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEKSLD"
     gene            4507..4962
                     /locus_tag="VHI09_000174"
     CDS             4507..4962
                     /locus_tag="VHI09_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ6120084.1"
                     /translation="MMIPTFGHKNAHNDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLGWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            5013..5315
                     /locus_tag="VHI09_000175"
     CDS             5013..5315
                     /locus_tag="VHI09_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEQ6120085.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(5580..8381)
                     /locus_tag="VHI09_000176"
     CDS             complement(5580..8381)
                     /locus_tag="VHI09_000176"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEQ6120086.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRNDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     DILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMMENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPVTDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEYESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGIAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESGK"
     gene            complement(8654..9412)
                     /locus_tag="VHI09_000177"
     CDS             complement(8654..9412)
                     /locus_tag="VHI09_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEQ6120087.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            complement(9422..10213)
                     /locus_tag="VHI09_000178"
     CDS             complement(9422..10213)
                     /locus_tag="VHI09_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEQ6120088.1"
                     /translation="MVSHSKIYQHFHQEEYSFIDRMSDMINRVEDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNNQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            complement(10213..10467)
                     /locus_tag="VHI09_000179"
     CDS             complement(10213..10467)
                     /locus_tag="VHI09_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEQ6120089.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            complement(10472..11128)
                     /locus_tag="VHI09_000180"
     CDS             complement(10472..11128)
                     /locus_tag="VHI09_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEQ6120090.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVTASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMS
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            complement(11140..11811)
                     /locus_tag="VHI09_000181"
     CDS             complement(11140..11811)
                     /locus_tag="VHI09_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEQ6120091.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTNDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKYMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIEEIGKMEKIQLVGLMTMA
                     PANASKESIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            complement(11814..13133)
                     /gene="ftsZ"
                     /locus_tag="VHI09_000182"
     CDS             complement(11814..13133)
                     /gene="ftsZ"
                     /locus_tag="VHI09_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEQ6120092.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            complement(13157..14521)
                     /gene="ftsA"
                     /locus_tag="VHI09_000183"
     CDS             complement(13157..14521)
                     /gene="ftsA"
                     /locus_tag="VHI09_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEQ6120093.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            complement(14732..15880)
                     /locus_tag="VHI09_000184"
     CDS             complement(14732..15880)
                     /locus_tag="VHI09_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEQ6120094.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKPKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLT
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMYDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQAPPFPETVLEQAHG"
     gene            complement(15881..16963)
                     /locus_tag="VHI09_000185"
     CDS             complement(15881..16963)
                     /locus_tag="VHI09_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEQ6120095.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIVAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDTNQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            complement(16963..18321)
                     /locus_tag="VHI09_000186"
     CDS             complement(16963..18321)
                     /locus_tag="VHI09_000186"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEQ6120096.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDENFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTKATVIPFSTQKVVDGAYLNDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARSVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRG
                     DA"
     gene            complement(18677..18928)
                     /locus_tag="VHI09_000187"
     CDS             complement(18677..18928)
                     /locus_tag="VHI09_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEQ6120097.1"
                     /translation="MFYLIMAVLIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQTPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
     gene            complement(19050..20891)
                     /gene="typA"
                     /locus_tag="VHI09_000188"
     CDS             complement(19050..20891)
                     /gene="typA"
                     /locus_tag="VHI09_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEQ6120098.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            complement(21074..21463)
                     /locus_tag="VHI09_000189"
     CDS             complement(21074..21463)
                     /locus_tag="VHI09_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEQ6120099.1"
                     /translation="MSPITLILWLLLVGIVGYHTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRTKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            complement(21473..22444)
                     /locus_tag="VHI09_000190"
     CDS             complement(21473..22444)
                     /locus_tag="VHI09_000190"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEQ6120100.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYDLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            complement(22449..22652)
                     /locus_tag="VHI09_000191"
     CDS             complement(22449..22652)
                     /locus_tag="VHI09_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEQ6120101.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            complement(22794..23321)
                     /locus_tag="VHI09_000192"
     CDS             complement(22794..23321)
                     /locus_tag="VHI09_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEQ6120102.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            23549..24176
                     /locus_tag="VHI09_000193"
                     /pseudo
     CDS             23549..24176
                     /locus_tag="VHI09_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(24258..25337)
                     /gene="rlmN"
                     /locus_tag="VHI09_000194"
     CDS             complement(24258..25337)
                     /gene="rlmN"
                     /locus_tag="VHI09_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEQ6120103.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(25341..25985)
                     /locus_tag="VHI09_000195"
     CDS             complement(25341..25985)
                     /locus_tag="VHI09_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEQ6120104.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPK
                     SKSKRVSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            complement(26409..27386)
                     /locus_tag="VHI09_000196"
     CDS             complement(26409..27386)
                     /locus_tag="VHI09_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ6120105.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDRVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTMYVCDSQNFDFESIQ
                     LEASYNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERETIRKR"
     gene            complement(27800..28837)
                     /locus_tag="VHI09_000197"
     CDS             complement(27800..28837)
                     /locus_tag="VHI09_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEQ6120106.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDADFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTIQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            complement(28824..29315)
                     /gene="coaD"
                     /locus_tag="VHI09_000198"
     CDS             complement(28824..29315)
                     /gene="coaD"
                     /locus_tag="VHI09_000198"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEQ6120107.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            complement(29305..29844)
                     /gene="rsmD"
                     /locus_tag="VHI09_000199"
     CDS             complement(29305..29844)
                     /gene="rsmD"
                     /locus_tag="VHI09_000199"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEQ6120108.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            complement(29967..30959)
                     /locus_tag="VHI09_000200"
     CDS             complement(29967..30959)
                     /locus_tag="VHI09_000200"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEQ6120109.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            complement(31272..32222)
                     /gene="arcC"
                     /locus_tag="VHI09_000201"
     CDS             complement(31272..32222)
                     /gene="arcC"
                     /locus_tag="VHI09_000201"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEQ6120110.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALMSTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKTLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYVNFNKPDQTKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
     gene            complement(32242..33573)
                     /locus_tag="VHI09_000202"
     CDS             complement(32242..33573)
                     /locus_tag="VHI09_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEQ6120111.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPEGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTDPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGCDKLSLEIGQAYNVVPARASYKG
                     NKLEALQKELDKLGFEYVVKEGELTVYGLAQHAKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAVQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            complement(33590..35083)
                     /locus_tag="VHI09_000203"
     CDS             complement(33590..35083)
                     /locus_tag="VHI09_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEQ6120112.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            complement(35253..36266)
                     /gene="argF"
                     /locus_tag="VHI09_000204"
     CDS             complement(35253..36266)
                     /gene="argF"
                     /locus_tag="VHI09_000204"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEQ6120113.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMDLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            complement(36390..36731)
                     /locus_tag="VHI09_000205"
     CDS             complement(36390..36731)
                     /locus_tag="VHI09_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ6120114.1"
                     /translation="MTDKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQTYGSKYLEDG
                     EYCQSLVKTIA"
     gene            complement(36831..38066)
                     /gene="arcA"
                     /locus_tag="VHI09_000206"
     CDS             complement(36831..38066)
                     /gene="arcA"
                     /locus_tag="VHI09_000206"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEQ6120115.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
BASE COUNT        11486 a         8041 c         6676 g        12030 t
ORIGIN      
        1 tttcataatt catagaacag atttataata ctctactcaa aaaatctttt gtacgttctt
       61 ctttagtcag atcaaaaagt tggttaggac ttccttcttc cacaataacg ccgccatcca
      121 taaacatcac acggtctgcg acttctttag caaagcccat ttcatgagtc acaataacca
      181 tagtcatccc agatttagcc aaatcttgca tgacagccaa gacctcaccc accatttctg
      241 gatctagagc tgaagttggt tcatcaaaga gtaaaacgtc tggatccata gccagtccac
      301 gcgcaatcgc aatccgctgc tgttgcccac cagaaagact agcaggataa gccttggctt
      361 tttctgataa tccaactttg tccaacaagc tcaaggctgt tttgtcagcc tctgctttag
      421 ccattccctt agttttgatt ggcgataaag tgatattttc taaaacagtc atattgggaa
      481 agaggttaaa ctgctggaaa accattccca ttttttcacg catgctgaaa atatcattct
      541 tcttatcggt aatatcaatc ccctcaaacc taatctgtcc cttagttggt acttccaaaa
      601 gattcatagt tcgaagtaag gtcgattttc cagaaccaga cgggccaata atcaccacaa
      661 cctctcctgg cataatgtct aaatcaattc ccttaagcac ttcattgtgc ccataatact
      721 tgtgcaagtc tttaattgaa ataatagcct cagtcatcaa tgattacctc cttgcgccat
      781 gtgacgttct aagactgcca ataactgtgc catcactgtt gtgaccatta agtagtaaaa
      841 agcagccacc agtaaagggg aaattggaga ataagtagcc gttaccaccg attgggcacc
      901 attccaaagt tccatcactc caatggtttg taaaagagca ctatccttaa taatggtaat
      961 aaattcattt cctaaggctg gcaaaatatt tttaaaggcc tgaggcaaaa tcacataacg
     1021 catggcattt tgaggacgaa tacctagtga ataagctgct tctaattgcc cttttggtac
     1081 agcctcaata cctgctctaa caatttctga aatataagca ccgctattca atgaaatgat
     1141 aataattcca ggaagtagtc ttgaaaagtc caagtctaaa accccaaaac caatagtagg
     1201 catattgtta aaatgcatcc aagcaaaggc aatcatgatt tgaaccacca taggtgttcc
     1261 ccgaaagatc caaacgtaaa gattaacgac ccaggtcaac ggcttcacat gactacgctt
     1321 aatcagggtt accaagacac caataagggt tccaaaaaag acaacgctga ctgaaatcat
     1381 aatggtgaca agtacaccgt agttaaagta ggcccagtat ttgggcaaaa atgacaaatc
     1441 catttatatc tcctcataag tcctaggttc attacaataa agataattat accatctgat
     1501 gaatattaat acaatacttt ttgacaataa aaaaacctgc ctcaggcaag tctctttact
     1561 atgtcaagct taatataata aatcgtagat ttccattgca atcaagtcaa tgctatcaaa
     1621 ggaatatgac tcacgcgcct caacatcacg caatgtgaaa acaggtccat tttcaggatc
     1681 atttgaaaag gcaacttctg caacaacaac accatcgcgt tcaaaatttc gcttgagatt
     1741 accgccatct gtcaccatca actccaaacg attgatgatt ctcaccaaat gtgattccat
     1801 ttcgtttctc ctattcgttt tttatccctc ttattttaac acaaaacaag ggaaactaac
     1861 aaggaaagta atatatttat tgatatttta cttttttact cttttcaatg attttaaaca
     1921 tttgctcatt ggaaacttaa taaagttact ctgtcatatt actctctaaa agctaacgct
     1981 aaagcgataa actttttact tgatttccca agccgtggtg ctataatatg aatataaagt
     2041 ttgaccattt ttgactaaat gactttgact tttagataaa ccctcggagg tatattatgc
     2101 tgtgtcaaaa ttgtaattta aacgaatcta ctattcatct ttatacaagt gttaatggaa
     2161 aacaaagaca ggttgatctc tgtcaaaatt gttatcaaat catgaaatcc gatcctgcca
     2221 attctatttt aaatggccta accccaggat atagagcaca agatagatcc acaagtcctt
     2281 tctttgatga cttttttggt gatttgaata attttagagc ttttggtaat cttccaaata
     2341 ccccacctac tcaggcaggg caaaatggaa atggcggagg acgctatggt ggtaactaca
     2401 acggacaacg acctgctcag ccacaaacac caaatcagca agcaaagggc ttgttagaag
     2461 agtttgggat taatgtcaca gatattgcaa gaaatggtaa tattgatcct gttattggtc
     2521 gtgacgaaga gattacacgc attatcgaga tcctcaaccg ccgtactaaa aataatcctg
     2581 tgctaattgg tgagcctgga gttggtaaaa ctgctgttgt agaaggtttg gctcaaaaaa
     2641 ttattgatgg tactgttcct caaaaactcc aaggcaagca agtgattcgt cttgatgtgg
     2701 ttagcctcgt tcagggaaca ggtatccgtg gtcagtttga agagcgcatg caaaaattaa
     2761 tggaagaaat tcgcaatcgc aaggatgtga ttctctttat tgatgaaatt catgagattg
     2821 tcggtgctgg ttctgcagga gacggcaata tggatgctgg taatatttta aaaccagcct
     2881 tggcccgcgg tgaattgcag ttggtcggcg ctaccacctt aaatgaatac cgtattattg
     2941 aaaaagacgc tgccttagaa cgacgtatgc aacccgttaa agttgatgaa ccttctgttg
     3001 aggaaaccat cacgatttta aaaggtatcc aaccgaaata cgaagactat catcatgtca
     3061 aatatagccc agccgctatt gaagctgctg ctcatttatc taaccgctat attcaagacc
     3121 gttttcttcc tgataaggct attgaccttc tggacgaagc tggttccaaa atgaatctaa
     3181 ccctcaactt tgttgatcct aaagaaattg acaaacgtct tattgaagct gagaatctca
     3241 aggcgcaagc tactcgagac gaagactatg aacgcgcagc ttatttccgc gatcaaatta
     3301 caaaatacaa agaaatgcag gctcaaaaag tcgatgagca agatattccc atcattactg
     3361 aaaaaaccat tgaagctatt gtagagcaaa aaactaatat tccagttggg gacttaaaag
     3421 aaaaggaaca gtctcaactc gtaaacttag ccaatgatct gaaagcacac gtgatcgggc
     3481 aagacgatgc tgttgataaa atcgctaagg ctattcgtcg taaccgtgtg ggattaggaa
     3541 ctccaaatcg ccctattggt tctttcttat tcgttggacc gactggggtt ggtaaaactg
     3601 aattgtctaa acaactagcc attgaactct ttggttcgac aaacaatatg attcgctttg
     3661 acatgtccga atacatggaa aaacacgctg tcgccaaatt agtcggggct cctccaggtt
     3721 atgtcggcta tgaagaagct ggacagctaa ccgaacaagt tcgtcgcaat ccttattccc
     3781 ttattctctt agatgaggtg gaaaaagccc atcctgacgt catgcacatg ttcttacaag
     3841 ttcttgatga cggtcgctta acagacggcc aagggcgaac agtcagcttc aaggacacca
     3901 ttattatcat gacctctaat gccggaacag gtaaaagcga agcttctgtc ggatttggtg
     3961 ctgctagaga aggacggaca agttctgtcc ttggtgaatt aagcaacttc ttcagcccag
     4021 agtttatgaa tcgtttcgat ggtattattg aattcaaagc tttatctaaa gaacatttgc
     4081 tgcatatcgt tgatttaatg ctggaagatg ttaatgagcg cttaggctat aatgggattc
     4141 atcttgatgt gacacaaaag gtcaaagaaa aattagtaga tttaggctat gatcctaaaa
     4201 tgggcgctcg gccgctccgt cgtactatcc aagattatat cgaagatgcc attacagact
     4261 attatttaga acacccaact gaaaaacagt tacgtgcact gatgacaaac agtgaaaaca
     4321 tcacgattaa agctgttaaa gagggagatt cttttcttaa cgaaaagtct ctagattaac
     4381 gttagctaag agagtttcta taaggagact ctctcttttt ctatcaattt tttattaaac
     4441 taaacaaatt gataagaata attagcaaaa atttagtata atgaagaaaa aggaaaggta
     4501 aataaaatga tgattccaac atttggacat aaaaatgcac ataatgacta tgtcacacgt
     4561 tacggtgtct atgctatcat tcccaatcac gaacaaacaa aaattattct tgttcaagct
     4621 cctaatggtt cctggttttt acctggtggt gaaattgaag caggtgaagg tcagcttcag
     4681 gccctagagc gtgaactgat tgaagaactt ggtttttctg ctactattgg ttcatattac
     4741 ggtcaggcag atgaatattt ctactctcgc catcgtgata cccacttcta ccatcctgcc
     4801 tatctttatg aagtaactgc atttcaagcc gtttctaagc ccttagaaga ttttaataac
     4861 ttaggttggt tttctcctat cgaggcaatt gctaaattaa aacgagagag ccatcaatgg
     4921 ggagtcaagg aatggcaaaa aaagcatcat tctactaact aaggctttaa attagtgcta
     4981 taataggtac aggaatacta aggaggacat ttatgagact catcaatacc actagcagtc
     5041 acccagaact catcagaaat cagctaaaaa acaccgatgc ctatttagtc gaagtatact
     5101 cagcaggaaa tacagatgtc atttttaccc aagcaccaaa acattacgaa ctacttattt
     5161 caaataaata ccgtgccatc aaagaggatg aacttgatat cattcgcgaa ttttttttaa
     5221 aacgtaaaat tgatcctaaa attgttattc ctggacaatc aaaaacactt cacacgaata
     5281 accttattga aatttcattt caaacttctg tataaacagc agccagctat tcagacaaaa
     5341 cttagccctt ctaaaagagc taagtttttt tattagcgaa aggtgaactc tatcttaata
     5401 gtccttatat gaagccagca attttcttaa aagaaattaa taaagtagtc taaatttctc
     5461 ctactttgtc tctcgttaaa gctggctaat ggacgtaatc agctctacta aaaaaccatc
     5521 aggtcctaag acccgatggt tgtcttctta ctcagtgtca cttcaagtct tgataagact
     5581 tatttgccac tctcttcaaa cccttcagcc acagcttctg ggaaattttc ttcaatgatt
     5641 ttagcgctgt gatcacagac aaatgcattg taagaacgca tgcgagtagt tgggtcgatg
     5701 cggcgtgaac gctcacaaac ttcacctatg gcatgttcta ccataaaggc aataccttca
     5761 aatgccactg catccgcagg cgcatctgcc aagtcagcaa tggttaattg agacacgatc
     5821 aaaagcaaag caatatcgct gtctaaagca gtcaataagg ttttcacttc ttcgctagcg
     5881 tagatggtca aatgggcttc caatgattta ccgataatct tagcatttcg cgcttcttcc
     5941 aaagcttttt gagcttgggt acgcaaagtc atgaaagcag accaagcttc taaaatatcc
     6001 tcttgagctg agaaagtttc tgccacaggc atttctgcca attgaacaaa tgcttctgac
     6061 tcatattcta gataagacca gatttcttca gtggtgtgag gcaagattgg cgtcaataac
     6121 ttggtaatct taaccaagat atcatagaag actgtctgca tgcgacgacg ttctaagcta
     6181 ttggcagctt caatatagac aacgtcttta gcaaaatcaa gatagaaggc tgataaatca
     6241 accgtaacaa agttcaccac tgctttgtaa atagccataa agtcataacg ctcataagcg
     6301 tcagtaatcg ttgctaccaa ctgattaaag acaattgtca tgtacttatc aacagctcct
     6361 aagtctgcat aagctactgt atccgtaaca gggttgaaat cagatgtatt agcaatcaag
     6421 aagcgaaggg tgttacggat tttacggtaa gtttcagaga cttgaccaag gatctccata
     6481 gaaacacgaa catcattatc ggtatccaca gaagctaccc agagacggag aatatctgca
     6541 ccatattgtt tagccacatc atttggagaa atgatattgc cttttgattt agacattttt
     6601 tcgcccttac catcaagaac aaagccttgc gataaaatag ccttatatgg agcatgccca
     6661 ttaacagcca cagatgtaat taaggatgaa ttgaaccaac cacgatactg gtcagagcct
     6721 tctaagtaga gatccgctgg ataggaaaga ttttctctag cattcatgac tccattccaa
     6781 gaagaaccag agtcaaacca aacgtccatg atatcagttt cttttgtaaa ctcgccattt
     6841 ggtgaccctg gatgagtgaa gccctctggt aataggtctt tagcttcttt ttgccaccaa
     6901 atgattgatc cattttcttg gaacaaatca gccacatgat ctgtcacttc tttagtcata
     6961 atagctgtgc catcttcggc atagaagata ggaagcggaa ctccccaagc acgttgacga
     7021 gaaataaccc agtcgccacg atcacgaatc atattgtaaa ggcgtgtttc accccatgat
     7081 ggatggaagg ttgttttttc aatttcatcc aaaatatctt gacggaaatc agaaacagaa
     7141 gcaaaccatt gcggtacagc acgccagatg attggttttt tcgtccgcca gtcaaatggg
     7201 taagagtgat tgatcacttc ttgtgccaat aaaagatcac caagtttatc aatgacaatc
     7261 ggcgttacct tgttataaaa ttgtccgtgg aaatctggac ctgcattttc catcattagg
     7321 ccacgttcat caacagtcac agcaacttcc agtttgtatt ttgtcccaac attgtagtca
     7381 tcctcaccaa aacctggtgc tgtgtggaca atccctgtcc ctgactcaag ggtaacatgg
     7441 tcaccaagga taaccaattc ttctacgtca gtatcccatg ggtgttctgt cacaatgtac
     7501 tctaaatcag ctcctttatg gctggctaaa gtttcaaaag attcccagcc aaattttcca
     7561 gcaaggctat ccaaaagacc ttctgccaca acatattgac ggtctgaacc agcaggtttc
     7621 accacaagat aatccatatc aggaccaaca gtcaaaccgc gtgatgctgt aaccgtaaat
     7681 ggtgttgttg tccaaacaac gatatacgta ttagtatcaa ggatgtcttt accatcttta
     7741 actttattgg cgtagtataa agaggttgaa tcaatgtcat ggtattcaat ttcagcttct
     7801 gccaaggccg attctgaaga ccatgaccaa taaacaggtt tagcaccacg atagatgtaa
     7861 cctttttcag ccatcgctcc aaaaacacga atttgatcag cttcaaactg tgggtccaaa
     7921 gtgacataag gattttccca gtctgccgaa acacctaacc gtttgaaatc atttcgctgt
     7981 ttatcaacct gacttaaagc gtattgacga cacatctcaa ggtattctgc taaatccatc
     8041 tccttacgtt tgattccttg cttagccaag acttgttcga ttggaaggcc gtgtgtgtcc
     8101 caacctggta cataaggcgc ttgaaagcca gacatggact tagaacgcac aatgatatct
     8161 tttgaaattt tattaagggc atgcccaacg tgaatattgc cgttagcgta tggaggtcca
     8221 tcatgaagat gaaaggcagg cttacctgca ttcaattctt gacgtttttt gtaaagctct
     8281 gcttgttccc aagctgcttg ccattgtggc tctttattag gaagacctgc gcgcattgga
     8341 aaggctgttt ttcctagatt aagtgtctct tttaatttca ttatttcaaa ctccttttaa
     8401 acaaacttgc gactataacg aaaaaagcct cgtctcaaaa aggacgaaag ctcgtggtac
     8461 caccttagtt cagagcgata atatatcata agacttaccg ctccctcttt gctcgtaagg
     8521 tgaacaaacc tcctcgctta ctgtagttca gcgaaaattt tagaaaatga taatgataat
     8581 acttagggaa tacgagactc tcatcatctc ccgcttgctg tgattataag caaatcatct
     8641 tgtttttctt tatttagata tttaatttaa acgtttgtgt ttcactgaga tttacctctg
     8701 gctcagtcgt agaatcagat tgagagtcaa gttggtaagc ctctaactcc ttattgcttt
     8761 catcaacacg acgttgcaat tcttctaact cttctggagt aaactgacgt gttgcatcaa
     8821 aagaggcact atcatcagat tcaggaatgt cttcattaag aacggtcttc acaacttcct
     8881 taaaagcatc gtcagaattt tgaagataga ttgcagtcgg ttgtagcagt tcatcccatt
     8941 caggtgaatt tgataagctt aattgtgact caatagatga gattaaacgt tggtggaaca
     9001 cacgtgtttg acgctttagt tcttctgttt caatcgcaac tcgcttagcc tcatcagttg
     9061 catcacgcaa catttgattg gctttagctt tagattcatc taataaatgc tgagcatcat
     9121 aagtcgcttt actaaccaag tttgttgctt ccgcattagc cgttgctttc actttttcag
     9181 ctgtttcttg agctaagatt acagactggc taagtgactc tttcatttca tcaaagtaag
     9241 ataatttttc ttcaagatct ttaattcttg cttcgttatc acgattttta cgtacaagag
     9301 cttcgtaatc atcaacaaca atatcaagga attcattgac ttcttcttca cagtatcctc
     9361 ggaattttgt cttaaaagtt ttatctttaa tttctagcgt tgtaagtgcc attgtatctc
     9421 cttatttatg tatcatttta cttagtgtta ttttttgttt gccatgtttg gtcactccat
     9481 tatctgctaa aagagtaaaa cgcccgtgac ctctgatact caccatatcc cctatgacta
     9541 gattatctga agctttatta gcgagtcgat agtttacctt aatcttattt gcttcaatta
     9601 atgctataac ttgcgttcga gattttttta agatagtagc aactacacca tctaatcgaa
     9661 aactagaaac tagaatatcc agggtctggt tgttatcaat agacctaatt aactgatcaa
     9721 agttaacttc tcttaatttc acactagttt tagctatttt agtaattgtt cctaaaaaat
     9781 aatccaataa ctcccgctta atcatgagct gggcatatcc catttcaaca aaaacatctc
     9841 caaataaatt tcgttttact cctaattcat taattaaagt tcctaaaatt tgactatgtg
     9901 tcaactgatt aaactttgcc tgataactta tctctactaa agctatttga aaatcacttt
     9961 gttctaagtc atagtaacca ggtgcaataa tgacacgacc atattcgctt gggtagtaat
    10021 ctgttgatac gaacattttt agatctgtta aagcaatcaa actttttaaa atcatcactt
    10081 ctctaggatt taaaaactca gtaacttcta aaagatagta atcttcaact ctattaatca
    10141 tatcagacat tctatcaata aaagaatatt cttcttggtg aaaatgctga taaatcttac
    10201 tatgactaac cattaaataa acacacggac caaaacttga gctaaaaaat tcaaactaat
    10261 aatgacgaca aaaatagtga agtcaagacc ggcaaattgt aaattaaatg ctctaaaagg
    10321 ttttaaaatt ggttcaacga taccactaat caaacgccca atttttgaat catatgcccc
    10381 aggaaaccat gacattaatg cgtaagcaat caataaataa gtgtaaactt tgatcagacg
    10441 aagaagaata gatagtatta atatcattta tttaccgcct tttcatatca aaatcaaagc
    10501 caatatcttg tgtagtatgt gggatagtca tttcttctat attgacggat acatttgacg
    10561 gagccagtaa gtacattgaa gagccgacct tttgtaagct accatagagc actttactag
    10621 caccatcaat aaaatctaaa caccgtctag cctgagcatc tagcataaac tgaaaatcaa
    10681 tcaaaacgca ttcattaact attaaaagat ccacaatttc ttgagcatcc tcatatttac
    10741 gaggatactt taaggcaata gttgtttgat ctgaacaagt tgattgttga tactgctcac
    10801 ggcgatttgc aataccagaa gtgctcatcc gttttcttct gtcaaccatt tcatggcctt
    10861 cttttggctg agactgctga taatagcgtt cagaagtacc atgttttgga tgcatttgtg
    10921 accttgtctg ttcttgggat ctcgcttgat gatcttgttg tacgtgattg tttctaggtt
    10981 cttgttttgg atgactactt gctctgactg attgttgtga tcttggaata acgttatcag
    11041 ttgatgctgt aacatcttct tcaacttcgt taacctcatc cgtgtcaaaa taagaaatca
    11101 tcttgttaaa tgtatcttta aaagccatct tattctccat tagtgaaaga aagctctacc
    11161 aatccgaata aaagttgagc cttcttgaat agcaattgga taatcgttac tcatgcccat
    11221 gctcaattct gtaaaaggca tattctttct tttttttaac tgcaagtttt ttcttaattg
    11281 atttgcttgt cgaaaaatag ttataatact ttctttactg gcatttgctg gtgccatagt
    11341 cattaaacca actaactgta tcttctccat cttacctatt tcttcaatcg cttcatcaat
    11401 ctcagaaatg ttaaaaccat gtttactctc ttctttagaa atattaactt gtagaaaaca
    11461 cttcacagga tggtcagctc tcttgttgat ttccaaagct aatctcacag aatctagagc
    11521 gtgaaaataa tcaacataat tgataacttc cttgacttta cgacgttgta aggtaccaat
    11581 taaatgccac tttactggca tatactttaa cgcatcatac ttttcaagaa atttatcaac
    11641 acggttttcg gcaatgtgct caattcctgc ttcgataagc tgacctgcaa ttgtactatc
    11701 cacatatttt gttacagcaa taactgaaac actatcatta gtcctatttg ctgcctctgt
    11761 agataagcgg atagtctcaa aaattttctt tttatttgtc agtaaatcca ttattaacgg
    11821 tttttaaaga atggaggcgt ttctaattca tcatcactgt catcgttagc tgagaacgtt
    11881 gacatattaa gatggttatc caattcacct tctgttggac gagaaatatt atcacgtctc
    11941 aaatcccaat ttccaaaagc tgaaccttga ttttgattat gattagaaat taccttttgt
    12001 gcacttggta tctcgcgaga ctcccccatg tcaaaatcaa aatttgagcg acgatcaaac
    12061 cctggttgaa ccgactgttt tgcttgatct gatgcatatt gtgcacccgc tacttgctgc
    12121 gcgttggttt gggtaaaagt cctaggctga cgaaaacctg aaacttgctc ggctttttct
    12181 tggcgcactc cagttgctac aacagtcaca cggatgtcat ctttcatagt atcatcaata
    12241 gatgttccta accaaatgtt aacgccttga ccagcagctt gcccaacaat ttcagaggct
    12301 tcttcagctt ctgtgagtgt catgtcgaga cctcctgtaa cgttcacaat aacgtcttgt
    12361 gcaccatcaa tagttgtttc taataggggt gaatagattg ccttacgcgc cgcctcaaca
    12421 atgcgctctt ctccagaacc aatcccaatc cccattaagg cattcccttt atttgccata
    12481 actgttttca cgtcggcaaa atcgagattg ataaggccag gactagtaat taagtcggtt
    12541 atcccttgaa ctccctgacg taaaacatta tcagcttcac taagtgcttc taataaaggt
    12601 gtctttttat caacaatctc aagaaggtta ttatttgaaa taattaacaa agtatcaact
    12661 tgttcacgga gttcttcgat accttcaata gcaaaattac cacgtttgtt accttcaaaa
    12721 ccaaacgggc gagtaacaac agctactgtc aaggctccca aacttttagc gatacgagca
    12781 ataaccggtg cagcccctgt cccagagcca ccacccatac cggcagtaat aaataccatg
    12841 tccgctcctg taagagcttc tgttaaaatt tcttcgcttt cttcagcagc tttacgtcct
    12901 acttcaggtt gtcctccagc accaagtcca cgagttaatt tagggcctag ttgaataacc
    12961 gtttcagctt ttgatgagct taatgcctga atgtctgtgt ttgctgcgat gaactcgaca
    13021 ccagcaacac cttcatcaat catacgatta atggcatttc cgccacctcc gccgactcca
    13081 attactttta taattgcacc ttgaattgat gcagtatcaa atgaaaatgc cattttatct
    13141 cctcacttta ttacttttaa tcaaacatac tcccaaatat gccacgaaca cgttctgaaa
    13201 ttttttgttt aggatcactc ggaacttgtg aatcatatgc tgtttgtgac gcttgttgtt
    13261 catagccaat ggtcgattct ggtcttcttg aatcatcata atctggtaaa taagattctt
    13321 gaccactgaa atcgataggc ttgcgtcgca aaagttcttc tcctgaaact gcagtttgtg
    13381 ctaaaacgtc tacttcagac atcataccaa catattctac caaactgata acgtttgaaa
    13441 acattggatt tctaataccg acttgatttg gaacatggag ctttacagtt actccaaaga
    13501 tttcttgtgc aatctctacc actccaggca tgattgcacc gccaccaatc aaaacaatgc
    13561 ctcctggtaa gtctagtaaa cgacctcttt ccaaatcttg cttcacacga tctaaaatat
    13621 gacgaatacg cgctgaaata atttcagata aataacgttc agttacctca acaggctctt
    13681 cactacctac cacatcaact tttactgttt cagttatact agcttctgat atctccgctt
    13741 gaccaaaatt aaacttaagt gcttctgcaa tagccaaaga cgtttttaat acttttgata
    13801 tatctttagt aatgtattcg ccgccttcag catatatatt ggtatactgc aattcttgtg
    13861 ctcgcataga agcgacagtt gtctgtccac ctcccatatc aattacagta gctccaaact
    13921 cgcgctcacc ttcgtttaaa atggttttag ccatagctaa cggagaaata atgatgtttt
    13981 caactttaat gcctgctctt tctaccgttt tacgcagatt atgtaaaatg gtgcttggtc
    14041 cagtataaat aagcccgcgc atctctaatc taatccccat cataccacgt ggatctcgaa
    14101 tgccctgaaa gccatccaca atgaactctt ctggaactaa agagataacc tctcgttctg
    14161 gtgtgatact ttttgttaaa gccgatttaa caacgctatc aacatcctca tcttttatct
    14221 ctttagactc acttgggaca ggaatcattc cttgtgttgg ttcaatttga agaaggtttg
    14281 ccggtagccc aacattaacc ttttcaattg tcatccctgc tttttcttct gcttgttcta
    14341 ccgcagtttt gatggcagtc gcagctgcct ctatatcgat tattatgcca tcttttacgc
    14401 cggtacttgg aacattacta acaccaatga cgttcatctc accagaaata aattctgcta
    14461 ctaaaacttt tatcgaactt gttccaatgt ccaaaccagt aaaaaagcca tttctagcca
    14521 ttcacttgac ctcacttact ttccaaactt ttgctattct aaaaactgct aaaaaacaga
    14581 atcattcaga aaatattata ccataaaaaa acgtaaaatg ttatcatttt acgttttttg
    14641 ttatataaac ttaatttttt taggcaattc attatgggtt agaatcgttg aatttctaac
    14701 gttttctttg ctttttcaac ttagatatta gctagccatg ggcctgttcg aggaccgttt
    14761 ctgggaatgg tggtgcttgc tgaggactac tctgatttgc tggtgtttct gaatcattgt
    14821 ctgtctgttc ctgatgttca ccaggcttat ctccttcttt atcagctgcg ttttgttctg
    14881 gcgtaagagg aacttcaggt tgattttcaa tctcctgtgt tgtagtataa attccaactt
    14941 ccatatccac tatactatca ttttcaaggt tttttttcaa tttttgataa tagggaagtt
    15001 tcaatgtgag ttgtgactgc ggtactctaa ctacattacc gtcatacatt tcaataagta
    15061 gtaaatccgc tgtcgttttg gaatttgcaa gagacactga cttgatattc ttgactaatt
    15121 ttttaggtaa tgtcgttaat tgcttaacta actgttggat cgctttctca tcttttaaat
    15181 taagaatcaa gaaagatttt ggtagttctg atgccctgac cttgtccaca cgttttccat
    15241 tctccaaaat aggctgaaaa ccgttttcga cttgtgcata agcgatgatt tcaaattcaa
    15301 taacgttaaa tagaaagtga ttaggaaatt ggtaagagag gtgtacagat ttcacccatg
    15361 gaatagtacg aagaatcggt cgttcatact gaccaggcga agttaacaac gttaaccaat
    15421 agtcagatgc tttgactttg ctagctttga ttaattcgtc aaggttcgtt tgatggtttc
    15481 ctcttacaga aaactctttc tttttgctat aaggagtgat cataaaaata gacaccgcca
    15541 taagtaagag cgcccccaaa agaacaggca aggatttttg aaaagcgatt tgtgtcgcgc
    15601 ttttttcctt gggttgtcta acttttttgg gttttttagg ttttgacgtc gtttctgact
    15661 caatttcctg actatcagtt ttctcatcag ttttaagctc aactgcttca gaagcatttt
    15721 gagcttgctg ctgcgctttt ttatcactca ataatttctc tttgagtttt ttttcttcct
    15781 cagcttgctg cttctttttc tttaaaaatt caatgttacg cttttgccac tctgtcaaaa
    15841 cgagcttgtc atcactttgt ttctctttat cttttgccat ttacttctcc ttaatcgcgg
    15901 agctaatatc agctctcaaa aggtcataaa agaagtccgg tgactgaatc tccttagttg
    15961 ccaacatagt agcctcataa tcagcctgat gttcaaacaa atcagccatt gcctgatcaa
    16021 aattatgcaa agttaaatca ggttcctgta attgtttagc gtagcccctc ttctcaaaat
    16081 aagtggcatt ttctaactga tcgccacggc tagcttcttt accaagagga acgatgaggt
    16141 gtagcttagc cattgccagt agctcaaaaa gtgtattaga gccccctctt gtcactacaa
    16201 ggtcagccat cgccatcaaa ggttggtaga gatcggtaac ataatctact cgatacagat
    16261 gagaactcaa ttcattaagg tgagggtctc ctgtaatatt gatgatatta taacgttgct
    16321 tcaattctgg atgatcacta ataaactgat taaacacatg cgcccctgcc gaaccaccaa
    16381 taaacaagag ggtttttagg tctctactaa aatactcttt caccgcctct aactgagttg
    16441 attcaggcat ttggttggta tctttgaaaa cctttgtcac cgctccaagg tgtttaactt
    16501 tagacaactg gtcttcctgc tcaaaagtgg tatacatggt agttgcaaat ttgtaggcaa
    16561 tcttgtttgc tagtcccatt gaccgatctg attcatgaat aaagactggt ttaccaagca
    16621 atttagcagc cacaactggc ggtactgaga caaaaccacc tttggaaaaa agggcttgag
    16681 ggcgaagctt ggcaacaata aagagagact gtaggaggcc aagtgcaacc ttaaaaacat
    16741 cagctagatt ttgccatgaa aaatagcgtc taagcttgcc tgtcgcgata gcatgaaagg
    16801 tcacgtcaag gcctgacttt tcaatttctg tatgttcaat gccattttta tcaccaatat
    16861 aatgtacttc ccaaccgtcc ttgataaatt ttggtatgag aatgaggttc aaggtgacat
    16921 gacctacagt tccaccacct gtaaataaaa tcttcttagg cattaggcat ctcctcttaa
    16981 acaatcaaag gttgccaaaa attcatcccc acgaacctca aaattaggat acatatccca
    17041 gctagcattg gctgggctaa gcaagatagt atcgcctgtt tgggccagct taaaagcaag
    17101 ctctgttgct tctgccacac ttctagcttc aagataagag acctctgctt tgttagcagc
    17161 tcgcttcata cgctctgcgg attctcccaa aataatcatc tgcttaagtc ctaaaaggtc
    17221 tggcaccaaa tcgtcaaatt cattgccacg atctagaccg ccagcaatca aaatcaagcg
    17281 actgttatca aaacctgata aagctttttg agtggctaaa atattggttg acttactgtc
    17341 attgtagaag gtaatatctt tgatttgacc aacccgttgc aaacgatgtt taacgcctcc
    17401 aaaatgtgaa aggcactggg caataatatc atcagcaata ccagataact tggcaactgc
    17461 aatagttgct agggcatttt caatgttatg gctacctggg acacctaagt cagttgcagc
    17521 tataatcgcc tgttctttaa aatagagtat tccatcattc agataagctc catcaaccac
    17581 tttttgagtt gaaaaaggaa tcactgttgc tttggtggtc ttagctagag ttgctgaaat
    17641 ctcttgatta gcatttaaaa taaggtagtc tgattctgtc atctgagctt gaatcatcca
    17701 tttagcagca acataatcct caaaactgcc atgatagtcc aggtgagtcg gcattaaatt
    17761 agtgatgaca gcaatatgag ggcgaaaagc attcactccc actaattgaa aagaggacaa
    17821 ttccatcacc aaagtatcac cagcaattgc tttttgaaca acttttgaag caggataacc
    17881 aatgtttcca gataagagtg cagattgccc gccagcattc aaaacatcgg caatcattgt
    17941 cgttgtggtt gtcttcccgt ttgatcctgt aatcccgata ataggcgctt cagatacgaa
    18001 ataagccaat tctacttcag tcaagatggg aatttccttt gcaagggcgc gtttaaccat
    18061 aggattatca taaggaatcc cagggttttt aaccatgtac tcaaagttct catctaataa
    18121 ttctactggg tggctaccac aaatgacctt aatcccctct tccaacaagg cttgtgccgc
    18181 tggattttgg tcaaatggtt tactatcatt aacagtcact aaagcaccaa gtttggtcaa
    18241 taattttgct gctgcttcgc ccgatttggc taaccccaat attaatattt ttttgttttg
    18301 aaaattactt atcactttca tctaagctca cactatccat tctatttgat ttctatttta
    18361 cctttattta gatgcttttt caacatttta aaaataccgt tcttgagttt agaattagaa
    18421 tccgtttcca atatacaaac atcatgccca tatttgtgag attattggta cgctttttta
    18481 agtaaaataa gataaaatac tagtcagagg tcttaactgt cactaccaag catctctttt
    18541 tagttgcaca ctcaatgaaa atcggctaaa agctttcttt cagaaaaaat gaactattaa
    18601 aaaaggactg atattgtcta aaacaaatca gtccttttat cgtataaaaa ccaaaatcat
    18661 caatctttaa gtatttttat ttaactctcg gttttttggt cattttacgc acatctttta
    18721 aagcaaaata gcccaatgcc accattccca tcccaataaa aaattcagga ggggtttgca
    18781 tgattttgat aaaactcaag cccgctaaaa caataagcag agcaaccaag ccaactaaac
    18841 caatcatccc aagggtgtta cggacacttt taggagccat gtatagataa taagatataa
    18901 tcaagacagc cataataagg taaaacattc cgtcctcctt tattttggtt tatattccat
    18961 aggaacttgt aaacgcaaaa agacctttaa aaaatgactt ttcttaaata tctttttgct
    19021 attttattta gttagtttaa cttcaaaagc tattcggctg attttttctt tttattagct
    19081 ttatcacgcg cagctttatt caagatttgt ttacgcaagc ggatagattc tggcgttact
    19141 tccatgtatt cgtcgtcatt caagaactct aatgattctt caagcgttag gatacgaggc
    19201 gtcttgataa ctgcagtttg atctttagtc gctgaacgga catttgtcat ttgcttagct
    19261 gttgtaatgt taacaccaag gtcgttatca cgtgagtttt caccaacaat catcccttcg
    19321 taaacttctg tacctggatt gacaaagata gttccacgtt cttcaatacg catgattgaa
    19381 tatgtcgttg ctttcccatt ttcaatagaa accaaggcac cacgatgacg gccaccaatt
    19441 tctccttgaa caaccggtag atactgatca aaagtatgat tcatgatacc atatccacgt
    19501 gtcattgaaa gaaactctgt tgaataacca atcaaaccac gtgcaggaat caagaaaatc
    19561 aaacgcgttt gaccattacc aaccatctgc atatcaagca tatccccttt acgttctgaa
    19621 agagactgga tgattgcacc ctgatattct tctggtgtat caatttgaac acgctcaaac
    19681 ggttcacatt tgacaccatc aatttctttg atgataactt ctggacgtga tacttgaagt
    19741 tcatagcctt cacggcgcat ggtttcgata agaattgaca agtgcaattc tccacgccct
    19801 gaaaccgtcc atttatctgg cgaatctgtt gggtcaacac gaagtgacac gtctgtttgc
    19861 aattctgcta aaagacgttc ttcaacctta cgtgacgtga tccatttacc ttcacgacct
    19921 gcaaaaggag agttattgac caagaaagtc atctgaagtg ttggctcatc aatacgaaga
    19981 attggcagag cttccacaca gtcagttggt gtaatggttt ctccaacaaa gatatcttcc
    20041 atacctgaaa cagcaatcaa gtcacctgct ttagcttctt gaatttcacg acgttccaaa
    20101 ccgaagaaac caaacagttt tgtaacacgg aagtttttag tggtaccatc aagttttgaa
    20161 agagttactt gatcacccac tttaacagta ccacggaaaa cacgaccgat accgatacga
    20221 cctacgaaat cgttgtagtc caaaagtgac acttggaatt gcaaaggctc atctgaatta
    20281 tcaactggcg ctggaatatg atcaataatc gtatcaaaga tcggtgccat agtatgctct
    20341 tggtcagcag ggtcatctga taatgatgat gttccattaa tagctgatgc gtaaacaact
    20401 gggaattcca attgctcatc atcggcacca agttcgatga ataattcaag cacttcatct
    20461 acaacttctg ctggacgagc tgaaggtttg tcaatcttgt tcaccacaac gatcgggata
    20521 aggttttgct caagtgcttt tttcaatacg aaacgcgtct ggggcattgt tccttcgtag
    20581 gcatccacaa caagaacaac cccgtcaacc attttcatga tacgttcaac ttcaccaccg
    20641 aagtccgcgt gtcctggggt atccatgatg ttaatacgaa catcgttata ggctactgcc
    20701 gtatttttcg caaggatcgt aatcccacgt tctttttcaa ggtcattgga atccatggca
    20761 cgctcttgaa gctctttacg ctcatcaaga gtatgggatt gttttaataa ttcatctaca
    20821 agtgttgttt ttccgtggtc aacgtgggca ataatcgcta cgttacggat atcgtttctt
    20881 aagttagtca tttgttcctc taagtaaata tattttaact agataagtat aacacaaaat
    20941 gttttaaaaa actggtttta atcaattgta aatgttttca gactttcagc aagtctggat
    21001 acagcaaaaa aaggaattaa ggtttttgag acagtgactg cctttattcc ccttaactcc
    21061 acgttactct taactaggtt gtttgtttaa ctttgccatc ccaataatca ataccgtctt
    21121 ttaagacata aacgtcttca aagccagctt ttttaagttt tttgaccgca ggcacacggt
    21181 actggggacg catattttcg tagataagaa caggtttgtc cttgcgaaga cctttgatag
    21241 cagcatcaaa ttgttgcgct ggaaaatttc gagctcctaa aatatgctta gttctaaaag
    21301 ccgctggctc tcgtaaatca atcaattgac cttgacgcat cacgtcttta aaagtctcgt
    21361 tatcaacctg ctttgccatt tttcgaaaag agaaatagtt ccatgtatga taaccaacaa
    21421 tgccgacaag aagtaaccat aagataagtg tgattggaga catatccccc tcctattttg
    21481 tgataaattg tcgagcaagg ctagctgcac cgataattcc agcatcattg ccaagctctg
    21541 caatcttaat tttagttgaa tacctcactt gtgggaaagt gaaggtcaca aagtattttt
    21601 caatacgtga gcgtagaaat tctcctgctg ctgacacacc cccaccgata accactgagt
    21661 ctggattcaa tatattggaa atatttgctg aagcaaggcc aaggtagtaa ccaacctttt
    21721 ccacaacaga atcagcaaag gaatcccctg cttcagctgc cataaaaata tctttgctgg
    21781 taacaccttc accattgtca atagctgctt tgatggctga atccccttca taagcttctg
    21841 ccaataaacg tgccactttg acaactcctg ttgctgaagc tactgtttcc aaacagccgt
    21901 gtgagccaca agtacaagca aagccatttt ctggctcaac aatcatgtgg ccgatttcac
    21961 cacctgctcc tgcaacacca tgaatcaagt taccatcagc aataatgcct ccaccgacac
    22021 ctgttccaag tgtcatgaag acgacatctg ggttattttc accagcacct acccaacgtt
    22081 caccaagggc agccacatta gcatcattgt caatggcaaa aggaatgccc aattcttttt
    22141 caacaacgga accgacttct tgggtttctt tccaattaag gttgaatgcc ccagtaactg
    22201 tattagtatc acggtcaacc gctcctggtg atcccatacc aattcccaca aaatcagcac
    22261 tgcttagatc atacaagtct aagcgatgtt tgatggaagc gatgatatcg ggaacaatat
    22321 gcttgcctcc ttctaaaata ttagtctcaa ttgcccattt ttcttggact tctcctgctg
    22381 ctgttaaaat tccaaattta atggtggtac cacctaaatc aatccccagt aatttttgac
    22441 tcatttgcct atccttcttt ttccaattct aatctatgct cacgtcgcaa aattaactcc
    22501 gcatttaagt aatccctctt atccaataag ccgctatcat agagacgctg aagttcaatc
    22561 ttcatcattt caatatcata gagacgtttg ccaaggtaaa caaaaatgcc aaagtttttt
    22621 agcaattgtt gcacatcata tagtgttttc atataagtag attttagcaa attctacggc
    22681 agatttcaag acaattatgg aatcgctttc tatgtttctg aaaatgaatc gatgattcca
    22741 taaaaaaaaa gccaatttta tcattgactt cttttatcct aaggcataat ccattagagt
    22801 gctgggcctt gtccacgctc tgcttggagc atccaaatgg ttttttcagc ctctgtctta
    22861 gctgctgtaa acaaatcgtt tgttccagca tcgccttctt catcggtaat atccaaacca
    22921 acttggtaaa ggctgctgag gtaaaggtaa acttcgacca atcttgctaa atgctgagca
    22981 acggttttat cgtaagtacc ttttgcttca tccaatttag agtgtttaga aaactcagct
    23041 agggtagaat aaggcgctcc gccaatagtg atcaaacgct cgctcatttc atctaaatta
    23101 gcattcaagc tatcaagtaa ttcatccatt ttggggtgaa ggtacaagaa acctggacca
    23161 cgcatatacc agtgtacttg gtgcacaata gatgcagcga cagataagtc agcgacagct
    23221 tggtttaaaa cagctttagt tttttcattt tttgatgctt cttttttaga aatattgtga
    23281 gttactgaag cataaatatt ttcaacgagt gtgtttgtca taataacatc tccttacttt
    23341 tcagcttaaa gggcaagaat ggagggggaa ctaaccctaa cgtgataaac ataagaaagg
    23401 gttaaggacc taatcctatt cgacaataac ggaataatcc taattggatg attatacact
    23461 taattagaat cattatcgtt ttatatctat attctacaat gattaaggaa agattgcaag
    23521 tatttgattt cttttcgaat aaataagtat gatagctttt tttagtatat gttcgggcat
    23581 cttattgacc catctttttt tactgtttac tactttattt tcctgataat ggtcaagaac
    23641 agctaagtaa acagactgtc attgtttgta accaactaac ttttgccaaa cgcgtttttc
    23701 ggcagctcca aaaccatttt ataactagcc aaaaaatctt ctttagctta gagataagtc
    23761 taataattat cttatgggct agtatggtac attgggttag tgctagttac tgttacttgt
    23821 tactcttttc tttattattt agtttatttg attggcgttc tcaaggatac ccttttatct
    23881 tgtggctttt tagctttgta agtctactcc tgttttattc gatcaattat ttatcattga
    23941 tattactgct actaggttta ttagctcatt taagaccttt ttcaattgga gcaggcgatt
    24001 ttttctacct tgctagctta gctttagtat tggatctaac gtcgttgatt tggctaattc
    24061 aacttgctag tttagcaggt attactgctt gcttactgtt aggcatcaag cgcatacctt
    24121 ttattcctta tcttagtttt ggattatttt ggattgtgct tctagagcat ttttaggcca
    24181 gataaaaaac ctagtcattt tagtgactag gaatattttg tcatcaagca ttgtcaatca
    24241 aagatatttt ttgggcttta ttttgtcgct gtaacctttt cgcgatcttt tttcatcgtt
    24301 ttagaacgta actggccaca ggctgcatca atatctgttc cgtgctcttg acgcaccaca
    24361 cagttaactc catttttctt tagcacatca taaaacgcta gaactcgctc tttaggactt
    24421 ctactatact gatcatgttc tgaaacagga ttatacggaa tcaagttaac ataagataac
    24481 ttacgaatag ttttagttaa gtctgctaat tcttgagctt gcttaatgct atcgttaact
    24541 tcatttagca taatgtattc aaaagtgaca cgacgattcg ttttttcaat atagtattct
    24601 atagctgaaa agagcttttc cagcggaaaa gagcgattta ctcgcataat acttgaacgt
    24661 aaatcattat ttggtgcatg caacgagact gctaaattaa cctgtactcc ctcgtttgca
    24721 aaatcccgaa tcttgtgagc taaccctgag gtagagactg taatatgacg ggcaccaatt
    24781 gctaagccat tatcatcgtt gatgacgcga agaaagcaca tgacattttt gtaattatca
    24841 aaaggttcac caatgcccat tacgacaaca tggctaaccc gctcaccttg ctttctatcg
    24901 tcaaaatatt tttggactaa catgatttga gctgtaattt ccccactgtt tagatcacgt
    24961 tgctttttga tcaatccact agcacaaaaa gtacagccaa tgttacagcc aacttgagtc
    25021 gtcacacaaa cagaatgacc ataatgttga cgcattaaaa ctgtttcaat taacatacca
    25081 tcgggcagtt caaataaata ttttactgta ccatcagctg actcttgtac aactcgctgt
    25141 tttaaaggat tgacacagaa actatcattt aaaatagaaa caaaatcttt agaaatattc
    25201 gtcatctcct caaaagattg aactcttttt ttatagagcc aatcccaaat ttgagtagct
    25261 ctaaactgtt tttggccgcg ttcaactgcc cacgcgatca gttcatcgcg tgttaagcta
    25321 taaatcgatg gtttcatgga ttatttatct ttctttctga tgataaaatg actaattttg
    25381 gtattaagtt gactagtctg attacgtttt gacttttctt tatgcttagt attacttcga
    25441 cgtttttgac tggtaaacgc ttggttttca ggttgctttt cttttgatac ccgcttcgat
    25501 ttactcttag gtgttgcggg ctgctgattt gaatagtttg gcttgcgacg atttgatttt
    25561 gatttaggtg atttctttcg tcttggttgg cgctcctctt caaatttaat atcttgtgga
    25621 tgaagatttt ctaaaacaaa ataagcacaa ccaaaattgc aaaattcttt aatataatct
    25681 tctaaacgtg agatgcgatt cgttttcttg atatcatcac tatccttata aaacccttta
    25741 agacgaagct gttcatttcc ccaatcgcca acaatatagt catactttag gagaacttct
    25801 gtataacgtt gaccaaagct agtcgtatca aaagcgttct taacatcttc taagagtaac
    25861 aaatcaatgc cttcagcctt aacttgctct tcaaaatgaa taaatttggg acctggaaat
    25921 ttattatagt tgtacatttc tggcgaaatt tcttttttca tttgacacct ttctgaaaaa
    25981 tccattagtt gtaactaaat cggttaattg tccagtgata actgattcgt catctttttt
    26041 atatctgtct atatcgcctt tatagtctta gataattatg gacacttgtt atcttttttg
    26101 cagtgttatc tgtaactcaa ccattataca ctattttttg acttttgtaa gtaaatgaga
    26161 gacttgctat ctcattactc actagctata aaatcacgac aattactata tttaggcttt
    26221 attgcgtcat catcgtgtaa cattcctcta tgttatcaaa aagacattaa gcttaaggtt
    26281 aagagttgca taagaatgcg ttttgactga tagcgatgaa agtaatgaca gataacatct
    26341 tttaaagtag aaggtaaatc ccaataaatc ttcatcatac ctgctaaaat ctggactaat
    26401 attattactt atcttttcct tattgtttct cgctcaacgt agtgaacagg caatttaatc
    26461 cgatttgctt tcacagttaa ttgactagca atagtataaa ctaattcggc ggcataacgg
    26521 cccatcaaat aagaggattg atgaatagtc gataaagaag gataaataaa ttgactcatt
    26581 aaaatgtcat cataacctat tatttgaaca tcttgaggaa tctttttacc ttgagaatgc
    26641 agttcatgaa tataagcaat agcatggata tcagaaggtg ctataatact atctattgtt
    26701 gggtaacact tgaggttgta actagcttct agctgaatac tttcaaaatc aaaattctga
    26761 ctatcacaca catacatagt cacgtcttgt ttttgaaggt aagaaaaact agcttcaaac
    26821 cgctcattaa tgttttcagc attatccagc ggacctctta ttaacagaac ttctttcgcc
    26881 cctgcttgcc agacagtctg agccgccaat aagccccctg ccctattatc tgaaaagaca
    26941 ccgtactggg tctcttggtc aacacgatct accactacaa ctggaatagc aagagtagga
    27001 taccgctttg taaagtcatg ggtagtaata atgccagcag cattgctttg aagaaggaca
    27061 tggacgtatt cttcttcaag agcttcgctg tctgaaatat ttcctagcat aacccgatac
    27121 cccttttctt ttaaatattc ttctgctcct cttgctaacc tcggaaaaaa aggattggaa
    27181 atgtctggta ataataaacc gactagttga ttttttttcg tcttcaatga ctgcgccaag
    27241 acatttggag tgtaatgtaa ttttgctatc gctgctttga ttttatgcct tgcatcatca
    27301 gatacatatc ctttttgaga aatatacctg gagactgttg atctagaaac acctgcttct
    27361 tcagcaactt gtttaatggt taccatagcc ttacctcctt gtaaaataag tttatgggga
    27421 tttttcaatc tcaagttttt aatttagttg ttataacgtt agcttgtcta ttgcaaggct
    27481 ttccagaata atagagtcat ggaatggact catgattatc tgcgcaagtt cttggatgag
    27541 ctagtgaaaa aaatcaattt tcactaggtg tttttagtat gctctatttc cagataatcg
    27601 actaaagttt actcaattct ctattgcctt tttaccacta gacttttata acatcttttt
    27661 tacgctgtca agttagatat gttttatgta aaaaatgatt gaacccaaaa gactggtcaa
    27721 ggatctgtct atctggttca accgtttttt atgagttaat agcgtgaata taattgaata
    27781 taatacattc atagttattt tatttgcgaa gataaaccag tgcctcttga acagtcgtaa
    27841 caggaacaat cttcattttg gtctttagtc gtttggctgc ccgtttggct tcttcgtaat
    27901 tacttatagc atttggatta acttttttaa tttccttatc aacaggatta ttcggaacaa
    27961 aaaatatatc tgcaccagct tcagctgctg caactacttt aagacctgca ccaccaatat
    28021 ctcctacttc gccatccttg ccaatagttc ctgtacctgc aattgtacgg cccttgcgta
    28081 aatcttcttt agttatttga tcatatatat caagagtaaa cattagacca gcactaggtc
    28141 ctcctactcc tttagtacta aagatcactg tgtcttctga attgacactt gtatgatcag
    28201 tcaaggcaat gccaatccca tttttcccat ttttcagttt gataatacgg ccaactcctt
    28261 ttttaggctt attatcactc gtaaactgaa tcgtaacttc atcccctagt tttaggtgag
    28321 aaacatagtc aataagttct gctgaactag taaactgttt accatttaca cctgttacag
    28381 tatctgctaa gtgtaacgtt cctttaaaag tagattcgtt gtttacgtct aaaacatata
    28441 cgcctttata atctaatgta actggttttc cagctaagga taaagcttga taaatagctg
    28501 cattttgtga tgtttccatg taaaattgat taattcgaag gaaatcagca tcgctgtatc
    28561 cgcctgttgt atcttctgct gtactaattt cagtaaacgg tgtcagccaa gcatataata
    28621 gctgagcgag gctggcacga ctaatgccca ctgcaacaaa ctggtaagct ccttttcgtt
    28681 tgtcttcttt gccattgact tgtaagacag tccgaatatc gtaagcgcct ccaggcattt
    28741 caatataata aggtagcgga aaaaataacg ctagcaacaa agagattaaa gctagcagac
    28801 ccactaacca ccatttgatt tttttaagtc ttttcattca ttttctccac ttgagcaata
    28861 actgactgag gaaccaaacc ttctaaggaa gactgaaaat gaattagctc tctcacacga
    28921 ctagagctaa gggcctgcca tttatttctt gatatcaaat agacggtctc aatatttgga
    28981 gctaaaaggt gattaaaata ttccaaattt tcttcatact caaagtcagt tgcatttcgc
    29041 aaacctctaa tcaagtgagt tactcgtaat tctttagcta catcaatagc taaacgttcg
    29101 tgcgaagtta ctacaattac atttgtaaaa tcagccagag cttgagtaag cattgcttta
    29161 cgaacttcta gtttaaaata gctttttttg gtaggattat caaaaatccc aacatatatt
    29221 tgatcaaata ggccacttgc ccgtttaaca atatcgagat gaccgtttgt aacaggatca
    29281 aatgaccctg tataaagtcc tattttagtt aacataaact gttaccttac taattccata
    29341 aattttttct ttccaaatac ctaacgttgc aatctctttt ggcaacaaga ctgttttatc
    29401 tgtctcacag acaaccataa cctgttcact caataagttt ttagctgcta aagcttcaat
    29461 agttgccaca attgtttctt tggcataagg agggtctaaa aagactaagt caaatcgacc
    29521 agtcaagcaa tcaatagctc gctcagcttc catttttaac aaggtaaacc gattctcagc
    29581 tttagtcatt ataatattgt cttgaataat agcttgcgct ttacgattct tctcaaccaa
    29641 aactgcagca gacatcccac gagaaacagc ctcaatggct aatccacctg aacctgcaaa
    29701 caaatctaaa acacgaccac cattaaagta aggtcctatc atattaaaaa tcgcacctct
    29761 aactttgtct gaagtagggc gtgttatctt tccatcgagc gtctttaaag gacgcccacc
    29821 aaattcacct gatacaactc tcataacatt tattatagca aatttctagc tctaaacctt
    29881 aaaaatgatt tcttttttca atatttctga tcatttattt aaagtctctt atacaagtgg
    29941 aaaacttagc aaaatgatgt gattacttat aaaatattat cgtagctatc acgaacttct
    30001 tggggccaaa cagaggtctg aacttctcca atatgttttt tacgcaacaa aaacatgacc
    30061 atacgtgatt gaccgattcc tccaccaatc gttagaggga aaagtccatt caacaaagat
    30121 ttgtgccaat caaaggctaa gcggtcttgg tcaccagtca tttccacctg acgttttagg
    30181 gcctcttcat ccacacggat ccccattgag gataattcaa aggctgaacc aagttggtca
    30241 ttccaaacca aaatatcacc attaagccca tgatagccat tttcagtttc tgttgtccag
    30301 tcatcatagt caggagcgcg gccatcgtgt ggtttaccgt caggtaaaac cccgccaata
    30361 ccaattaaaa agactgctcc aaattcttta gtaatggcat tttcacgttc tttgggcgtc
    30421 aaatcagggt atttcgctac caattcttcg gtatgaatga aggttatttt cttaggtaaa
    30481 acagcctcaa tatcataacg agcttctaca gctaattcag ttagccgaat gaccttgtaa
    30541 atggtttcaa cggtttcttt gaggtaagcg agattacgtt tgccatcagg gatgactttt
    30601 tcccagtccc attggtcaac atagacagaa tgggtttggt caagtgaatc ctcatcaggt
    30661 cggagagcct tcatattaac aacaaggcct tcaccttcat taaacccaaa acgggctaag
    30721 gtgtgacgtt tccattttgc taatgaatgc acaacttcaa aggttgcatt aggaattttc
    30781 aagacattga cagatactgg attttcagta ccggacaaat tatcttgcat accatccccg
    30841 acccgactta aaatgggtcc ttgcacttca acaacatcta acttagcaat taaatactga
    30901 gtgaacgtat ttttaacaaa ggaaatctcc tcttgttgat gaataaaact tttcttcata
    30961 gttcttcctt cttttttggt aatctttttg ggactaatga actcaacgac ttagcagttc
    31021 tctttatacg ttgtctactt ttgtttattc taatattttg aaaaataaaa aatagtatgg
    31081 ttattataca cctatcaaag ccaaaagaaa aggctaattc tcaataaaat tagccttttc
    31141 taatactatt attgtttttt attatggcac actcttttcc tttaaaaaca gtataaactc
    31201 tttatgtcag tcgtactctt cagaaaatac cattaaatga gtcttttaaa cagaactaaa
    31261 aatcctcttg attaccctgc gataatttgt gttccagcgt ttgctgatag tacattgtca
    31321 atattttcga gcgatgtgat aatggcttta gcatttggtt tgttttcaac aaaagcaatg
    31381 gcagcttcca cttttggtaa catactacct ggtgcaaatt ggtcttgtgt gatgtactct
    31441 ttcatttgag agaccgttac ttcttccaat tttgtttgat caggtttgtt gaagttaaca
    31501 taaacattgt caacccctgt caaaacgata aagaggtctg catccaccaa ttctgaaagg
    31561 gtttggctag caaagtcctt gtcaataacg gcttccacac ccgtaagggt cttgctagtt
    31621 gcatcttcca caacgggcac accgccacca ccagcactaa ccacaacaac gccagaatca
    31681 accagacttc tgatgacgtt ggcttcttta ataccaactg gttttggtga tggcacaacc
    31741 ttacgccagc cacgacctgc atcttctttg aaacttgcac cagactccgc catttgtttt
    31801 ttggcatctt cttcggtaag aaatggacca attggttttg ttgggttttc aaaagctggg
    31861 tctttggcat ccacgatcac ttgagtgaca acagctgcca cttctttttg aataccttgg
    31921 gcttgcaatt cattatcaag ggcattaact aaccaaaaac cgatgctacc ttctgtcatg
    31981 gcaacacaag tatccaaagg catagccgga ttcttttcag aatctgctgc tgcttgttgt
    32041 aataagagat taccaacttg tgggccattg ccgtgggtca cgatgacttc atgaccttct
    32101 ttaattaatt taaccaatga ttttgatgtg gacatcaagg cttcttgttg tgcttttgct
    32161 gacgcatctg ttgaaagaat agcatttcct ccaagtgcga ctacgatttt ttgtttcgtc
    32221 ataattactc ctcatatctt gttatcgcgt tagtcggtaa atagcttccg cataaatatc
    32281 catagcgcgg taagcatccg ctaaaacaat atgttcattt tcttggtgtt ccgtttgaac
    32341 agcatcaggg aaaagggcac caaaggcaac acaattcttc attgttctag caaaggttgc
    32401 ccctcctgat gattgggcag ggctttgatc acctgttttt ttccgataga cagataacaa
    32461 ggttgttacc aattcactgt ctaatggcac atataatggg gccaaataat caaattcttc
    32521 ataggttagg ccgtattctt gtgctttttg gcttaattgt tggactaatt tttccttatc
    32581 tgccaaaact ggaatccgaa tatctaggcg aatttctgcc ttgtctttgg ttaaggtaag
    32641 ccctgcggca ttaaagctta atttgcctga aggttcatct tcgattctgc caaaaatatt
    32701 aagtgctctg ccatcttcat caatgacatt agcaagaaaa tcaagggttt tgttaggttc
    32761 aaggaccact aaagccttgg caagcctaat catagcgttg atgccatcgg gagcatcttt
    32821 agcatgttgg gcgagaccat aaaccgttaa ttctccctct ttgacgacgt actcaaagcc
    32881 aagcttatct agttcttttt gtaaagcctc aagcttattg cctttataag aagctcttgc
    32941 aggaacaaca ttgtaggctt gtccgatttc aagtgacagt ttatcacaac ctttccccac
    33001 taattttgcc tgaagtaggc ctttttcggc ataggttaaa gggaaacttg aatcaggagc
    33061 aaacccaaag gtagcctgtt cttctacatc attgtagcga ttcatacacc gccacaaagt
    33121 ttcttcatct gttccgaaaa taaaacgaat gcgtttatta aaggtaacgc ctgcatctat
    33181 caaggctttg gtggcaaata gcgccatcat agtaggccct ttatcgtctt gagtgcctcg
    33241 accaaataaa cagccgtctg cctcaacaca gtcaaaagga tctgtcttcc ataactgacg
    33301 atctccttct ggaacaacgt ccaaatggca aagaatagcc aagacctctt tttggtcacc
    33361 taactcagca tagccatagt aaccttcagg atcaatataa gtatgaaaac caagaccttg
    33421 acataagtct aaggttgctt cgagaacatc ttgaatggcc tgtccaaaag gtgtgccatt
    33481 ttctccttca tgacaaacag aagggtaaga aacgatctgt ttaatggcag ctacacaggc
    33541 ctcttgatgt ttaggtgtga tataggattc cataggaacc tcctatctgt taaaagaatg
    33601 ttgcaactac aagtaatagc acgcttacaa gcatcaccat tacaataaat ttaccaatga
    33661 atttccacca agtaccaagg tcaacgcgac caagcgcaag tgctcccata acgattgctg
    33721 aagttggaga aatcatgttt aagattccag aagctgactg gaaggctgta ataacaaggt
    33781 gagcagggac atttgagaat tgtccaagcg gtgccataat tcccattgta gccccagcaa
    33841 gtcctgatgt tgatggaatc aagaaggaca ttgggaggta gaaaatataa gctaatatca
    33901 caaaaacttg agaagataaa ccagaaagac ttgtttcacc taagtgtaag atagtggctg
    33961 taatcatacc accattcatg ataacttgaa taccgcgtgc aatagcacag atcatggcaa
    34021 cacctaaaaa ttcacctgca ccagtcaaaa aagagctaaa aaagtcttct tcactcatac
    34081 gatacacaat agcaactaag acacccatca tgataaagag catggtgatt tctgggaaat
    34141 accaagtacc aaatgctccc atggttttcc caataactcc ccctaagata ggcattgtgg
    34201 ttaaccaagt attgatatta gtaaagaatt tgatgccaaa atcttcccac ggaatcaaac
    34261 taagaatcat aatgacaaag gtcaaagtaa aaatggtcaa aacgtttcgt tggcgcttgt
    34321 ttaagtcttc tccagaattt tgcaactgga agagttcctt atgctcttct tccttgtctg
    34381 caaccaatga tttacttggg tcttcttcaa tcttactagc gtaattgtaa acaaaccaaa
    34441 tagacatgcc gacaagaata acccattgga taacacgcca aatcattcca tctgcaatgc
    34501 tgacaccagc agcatcagct gctacaccag ttgcaaatgg gttaatagtg gaggctagac
    34561 acccaatttg agaaccaatc aaaataatgg ctacagcaac aatactgtca aaaccaaccg
    34621 caatcataac aggaattaaa agtgggtaaa aggccatggt ttcttccccc ataccatagg
    34681 tcgttccacc tagagcaaac aaagggatta aaatagcaat cagcattttt tctctacctt
    34741 tgtttttacg aaccactgac gcaatccctg tgtcaagagc acctgttttg ttgacaactc
    34801 ctaaaaagcc cccaaccatt aagataaaga aagacacttg aatagcacca tcagtgcctt
    34861 caacacctaa catgcctcga acgggagcca tcaagatgtc aaaaaatcct tgaggattgg
    34921 atgctactgt tttgtaggtt cctgaaataa caccaccgcc cttagcggtt tcgtaggcgc
    34981 cagctggaat aaaccaagtt aacactgcca tgatggctat aatgataaaa agaacggtgt
    35041 aagaagaagg aattctaaaa ccccgttttt tttcttctgt cataatgatt acctcttacc
    35101 ttttcataaa aataagccgt tctagttccg gtcaaggacg cgaaaaccag aacgacttgt
    35161 catgtagtta aagtacgttt ttaagtagtt aaattgttaa attaaagggg tcacattgat
    35221 atgtgaaatg agttaaggag taccagtcct tattacactt ttggaataaa gaggttacca
    35281 agggttgctg ccataaccgc tttgatagtg tgcatgcggt tttcagcttg gtcaaaatga
    35341 cgagcgtatt tgctgcggaa gacttcatca gtgacttcca tttctttcac gccgaatttt
    35401 tcagcaacgt ctttaccata aacggtattt gtgtcatgga aagctggcaa gcagtgcaag
    35461 aagataagct tgtcatttcc tgctttttga acaaggtcca tgttgacttg atatggttga
    35521 agcaattcaa cacgttcttt gaatttgtct tcttcaccca ttgataccca aacatctgtg
    35581 taaagcacgt ctgcgccttt aacagcttcg tcagcatctt ctgtgataag gatacgagcg
    35641 ccactttctt tagcatagcc ttcagccaat gtcacaatct cttcttctgg gaaaagttct
    35701 tttggtgaga agatatgaac attaacacca aggatagcac cagtcacaag aagggaattg
    35761 gcaacgttat tacgcccgtc accacagtaa acaagggtaa ggccttcaag tttgccaaag
    35821 ttttctttca ctgttaaata gtcagcaagc atttgggttg ggtgccattc atcagtcaaa
    35881 ccattccaaa ctggcacacc tgagaattct gccaattctt caaccatgcg ttggctaaag
    35941 ccacggaatt caatcccatc aaacatacga cccaatactt tggcagtgtc ttcagttgat
    36001 tcttttttac caagttggat gtcattggca ccaaggtatt ctgggtgagc acctaggtca
    36061 atggctgctg ttgtaaaagc tgcacgcgta cgagttgatg ttttttcaaa caagagggca
    36121 atgtttttac cttctaagta gtgatgaggc acaccacgtt ttttcaaatc tttcaaatga
    36181 gctgaaaaat caataaggta ttctaattca gcgcgtgtaa aatctttttc tgctaggaag
    36241 ctacgtcctt gaaatacttg tgtcatctat tgtctccttc gagtattaac ttaatctttg
    36301 ggttcattat tttttgtgag atagagatag cagcctaata gaccaccaac aatcgcaact
    36361 agcattgcta tgtccatatt cttctcccat taagcaatgg ttttaacaag ggattgacaa
    36421 tactcaccat cttctaaata cttggaacca taggtctgat aacctaggtt ttcataaaag
    36481 gtttgtgctg tcagttcact gtgaatggtt agttggtaaa agccttctcg tctggtataa
    36541 gtttctagcg cttcagtgac tttggcacca taaccgcaac cacaataatc cgctaaggtt
    36601 acaatgcgtg tcaatcgtgc ttctattttc gtttcagcta aaaattgtcc tgttgaaacg
    36661 ggctggtcat tgtcataaac gactgcataa accgttcttg tttcgtcatt ggcatcaaat
    36721 ttatctgtca tgacaaaatt tcattgaagg acaaagacac agaagcgaag ataaacacta
    36781 gctgcccttt aaaaaggctt ctgacctata accaccttta ccatagctta ttaaatatct
    36841 tcacgttcaa atggcattga catacaacga ggtccaccac gaccgcgaac caattcactt
    36901 ccgtgaatct tgatcaattt caagccttta gattcaagaa tagcattggt aatggtgtta
    36961 cggttataaa ccacaaccac acctggtgcg atagtaaggg tgttagaacc atcgttccat
    37021 tgttcacgac ctgctgctac taagttgtca ccaccacaac ggataaggtc aactttttca
    37081 acaccaaggt tagcagcaag aagttctgct aaatcacctt tttcttcaac gatatgaagt
    37141 tcttcattat cgtaagtgac agagtaaaca cgaaggtcgc cttcaatttc tgggtgaatg
    37201 gtaaatttgt catagtcaac catggtaaag acagtgtcta agtgcataaa tttacggtta
    37261 tttgcaaatt caaaggccaa tactttcttg aagccaaggt tttgtttaaa gatgttaacc
    37321 aacaattttt caatagaagc agcatctgta cgttgagaaa taccaaccgc aagcacatct
    37381 tttgaaagaa caagttcgtc cccaccttca atgcgagtgg tttcattacg gtcataaacc
    37441 ataggcactt tgccaccccc ataaattggg tgatgagtga aaatgtattt accataaagg
    37501 gtttcacggt tacgtgtttc tgagaacatg tggttaagag aaacacctgt accgatagtt
    37561 gcgaatgggt cccgtgtgaa ataaaggttt ggcattgggt cgatggcaaa tgggtaattg
    37621 gattcaacca agtcagtcaa gcctttttca gatgctggga tctctggaag ttcagatttt
    37681 tgaactcctg ccatggtttt ttcaatcaat tcttgattgt cttcgatagc catcaacaat
    37741 tcacgaatgg cttttttagt agctcttcca cggatattag cttcacttag gtattcatcg
    37801 atgaaagctt cgcgaatttc tggagtgaca agagattctg cagctaaggt ctcaaggtac
    37861 aagacttcaa taccttcgtc acgcaaagct tgggcaaaag catcatgttc tttttgggca
    37921 tcctctaaga atggaatgtc atcaaacaac aagcgttcca agtagtctgg cataagattt
    37981 tcaatttctt taccaggtct gtggagcaac acttttttta attttccaat ttcagaataa
    38041 acatgaattg gtgtttgagc agtcataaga attacctctt tctaattggt ttttgtgaat
    38101 tcaccgtcaa gattcttaac tcttgcttat attcctagtg tactccttaa aaaagataaa
    38161 aatagggtat ttctcacagt cattttgagt aatcgcttac attttttgag cgttgttttt
    38221 atttttatag att
//
LOCUS       DAUELZ010000006        37989 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.006, whole genome shotgun sequence.
ACCESSION   DAUELZ010000006 DAUELZ010000000
VERSION     DAUELZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37989
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.006"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            29..1225
                     /locus_tag="VHI09_000207"
     CDS             29..1225
                     /locus_tag="VHI09_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ6120116.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPACKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMIIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTAVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVVS"
     gene            1697..2545
                     /locus_tag="VHI09_000208"
     CDS             1697..2545
                     /locus_tag="VHI09_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ6120117.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKVSYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(2881..3777)
                     /locus_tag="VHI09_000209"
     CDS             complement(2881..3777)
                     /locus_tag="VHI09_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEQ6120118.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYLGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            4075..4827
                     /locus_tag="VHI09_000210"
     CDS             4075..4827
                     /locus_tag="VHI09_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEQ6120119.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAYYDTLFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            4812..5762
                     /locus_tag="VHI09_000211"
     CDS             4812..5762
                     /locus_tag="VHI09_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEQ6120120.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKAYYPSIADATVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKALNHLTQKENKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTIERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
     gene            complement(5942..8269)
                     /gene="pflB"
                     /locus_tag="VHI09_000212"
     CDS             complement(5942..8269)
                     /gene="pflB"
                     /locus_tag="VHI09_000212"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEQ6120121.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            8478..9572
                     /gene="dinB"
                     /locus_tag="VHI09_000213"
     CDS             8478..9572
                     /gene="dinB"
                     /locus_tag="VHI09_000213"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEQ6120122.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDVVEPMSIDEAYLDVTNNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGVTMTNLEDKVADISLDL"
     gene            complement(9665..10147)
                     /locus_tag="VHI09_000214"
     CDS             complement(9665..10147)
                     /locus_tag="VHI09_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120123.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSVLNVLAIIAIIIFCTQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTLSMVGTILILIQAALYGFAFYKAKNLNEKGDDTDQAML
                     "
     gene            complement(10238..12691)
                     /locus_tag="VHI09_000215"
     CDS             complement(10238..12691)
                     /locus_tag="VHI09_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEQ6120124.1"
                     /translation="MEHFFTGTVDRIIFENAANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFALQAFEKYKEEALDLVKENPYQLVEDLQGFGFKMADALAENLGIESDSPKRFR
                     AALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QPNQELKSKEQTSLFSNTATLEDDSQKSSSQSTNSNPTENSQSDNDDFRLTPENYSTI
                     DSMIGLTESDIALFFQKKS"
     gene            complement(12749..13342)
                     /gene="lepB"
                     /locus_tag="VHI09_000216"
     CDS             complement(12749..13342)
                     /gene="lepB"
                     /locus_tag="VHI09_000216"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ6120125.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVVGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            complement(13353..14255)
                     /locus_tag="VHI09_000217"
     CDS             complement(13353..14255)
                     /locus_tag="VHI09_000217"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEQ6120126.1"
                     /translation="MNTLVLKIDAILSKHLKKQLAPYTINSQNTYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHSFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNAISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKSHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            14412..14720
                     /locus_tag="VHI09_000218"
     CDS             14412..14720
                     /locus_tag="VHI09_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120127.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            14723..15268
                     /locus_tag="VHI09_000219"
     CDS             14723..15268
                     /locus_tag="VHI09_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEQ6120128.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            15417..17756
                     /locus_tag="VHI09_000220"
     CDS             15417..17756
                     /locus_tag="VHI09_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEQ6120129.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPKLERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHTFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISDDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADRNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFSLVRVQEEQ
                     KVKNKQINVVKKADSSGPRARLDLRGKRYEAAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            17760..18260
                     /locus_tag="VHI09_000221"
     CDS             17760..18260
                     /locus_tag="VHI09_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ6120130.1"
                     /translation="MESYEHFYAKLSQPFRKTPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            18341..18655
                     /gene="trxA"
                     /locus_tag="VHI09_000222"
     CDS             18341..18655
                     /gene="trxA"
                     /locus_tag="VHI09_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEQ6120131.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMLKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(18707..19294)
                     /locus_tag="VHI09_000223"
     CDS             complement(18707..19294)
                     /locus_tag="VHI09_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6120132.1"
                     /translation="MEIGQQMIRYRKQQTLSQEELVEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQPYKEILAVSNGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVVIVFASRFMFTWLFH"
     gene            complement(19471..20595)
                     /gene="mutY"
                     /locus_tag="VHI09_000224"
     CDS             complement(19471..20595)
                     /gene="mutY"
                     /locus_tag="VHI09_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEQ6120133.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            20793..21086
                     /locus_tag="VHI09_000225"
     CDS             20793..21086
                     /locus_tag="VHI09_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120134.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            21259..21549
                     /locus_tag="VHI09_000226"
     CDS             21259..21549
                     /locus_tag="VHI09_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEQ6120135.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            21571..22062
                     /locus_tag="VHI09_000227"
     CDS             21571..22062
                     /locus_tag="VHI09_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ6120136.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            22226..22465
                     /locus_tag="VHI09_000228"
     CDS             22226..22465
                     /locus_tag="VHI09_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEQ6120137.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(22596..23252)
                     /locus_tag="VHI09_000229"
     CDS             complement(22596..23252)
                     /locus_tag="VHI09_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEQ6120138.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQRAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLVLLIIGGVSLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(23384..24328)
                     /locus_tag="VHI09_000230"
     CDS             complement(23384..24328)
                     /locus_tag="VHI09_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEQ6120139.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            24531..27359
                     /gene="uvrA"
                     /locus_tag="VHI09_000231"
     CDS             24531..27359
                     /gene="uvrA"
                     /locus_tag="VHI09_000231"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEQ6120140.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPIVRRKK
                     GQHKTIFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLKEGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIHGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            27475..28548
                     /locus_tag="VHI09_000232"
     CDS             27475..28548
                     /locus_tag="VHI09_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ6120141.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRHVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLEADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRLIKDTSEIDTIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIVASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIAKASAGMTYSDFDGIPRQL
                     IAEAGYGSRFTHGIGHGIGLDIHENPFFGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITKTGCQVLTLAPKELIVL"
     gene            28583..29044
                     /locus_tag="VHI09_000233"
     CDS             28583..29044
                     /locus_tag="VHI09_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEQ6120142.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGITKITYKAHYRPHPFAIELMEKKGVAYVQHDVPQIVLGGDKEQ"
     gene            29140..29697
                     /gene="efp"
                     /locus_tag="VHI09_000234"
     CDS             29140..29697
                     /gene="efp"
                     /locus_tag="VHI09_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEQ6120143.1"
                     /translation="MIEASKLKAGMTFEAEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            29743..30132
                     /locus_tag="VHI09_000235"
     CDS             29743..30132
                     /locus_tag="VHI09_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ6120144.1"
                     /translation="MTTEYIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            30125..30577
                     /gene="nusB"
                     /locus_tag="VHI09_000236"
     CDS             30125..30577
                     /gene="nusB"
                     /locus_tag="VHI09_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEQ6120145.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVMGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(30718..31683)
                     /locus_tag="VHI09_000237"
     CDS             complement(30718..31683)
                     /locus_tag="VHI09_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ6120146.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEIIKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(31688..33127)
                     /locus_tag="VHI09_000238"
     CDS             complement(31688..33127)
                     /locus_tag="VHI09_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEQ6120147.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGRYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIDDLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQSHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVEGQAFGHYYEMRY"
     gene            33369..35252
                     /locus_tag="VHI09_000239"
     CDS             33369..35252
                     /locus_tag="VHI09_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ6120148.1"
                     /translation="MDNRQIATEVIEALGGRENVRSVAHCATRLRVMVYDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEHGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSILGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAAIAAQAGATLAVAVKTKSPKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGQLLQYLVTMLVGLGVAFAIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKRGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHILIGDQVA
                     LIK"
     gene            35483..>37989
                     /locus_tag="VHI09_000240"
     CDS             35483..>37989
                     /locus_tag="VHI09_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEQ6120149.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEEKTVQVQ
                     KELSSIDSLHYLSENSKKEFKEELSKAGQESQKVKEILAKAQQADKQAQELAKMKIPE
                     KILMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLF
                     WKELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIV
                     DEYVYKYNLDGLDVDVEHDSIPKVNGEASDENLKRSIDVFEEIGKLIGPKGVDKSRLF
                     IMDSTYMADKNPLIERGAPYIDLLLVQVYGARGEQGEFQNDTKLVTETPEERWQGYSK
                     YIRPEQYMIGFSFYEERAGSGNLWYDINSRKDDDKANGINTDITGTRAERYARWQPKT
                     GGVKGGIFSYAIDRDGVAHQPKQIAEKDKQNVKNNQPQIPEITDNIFHSDYSVSKALK
                     TVMLKDKSYDLIDEKDFPDKALREAVMAQVGTRKGDLERFNGTLRLDNPAIQSLEGLN
                     KFKKLAQLDLIGLSCITKLDQSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSL
                     KVSGLTGLKELDLSGFDRETLAGLDAATLTSLEKVDISGNKLDLAPGTENRQIFDTML
                     SIISNHVGSNEQTVKFDKQKPTGHYPDTYGKTSLRLPVANEKVDLQSQLLFGTVTNQG
                     TLINSEADYKAYQNHKIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLAT
                     DKEETYKVDFFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADPVNARKVFD
                     GQLGSETDNISLGWDSKQSIIFKLKEDGLIKHWRFFN"
BASE COUNT        11513 a         6700 c         8003 g        11773 t
ORIGIN      
        1 tatgaaaata gagaggagtt cattttcaat ggaagaaaaa ttgtttaaca aacattttgt
       61 ggccatcaca gtaataaact ttatagtcta tatggtctac tatcttttta cggttattat
      121 tgcctttgtg gcgactagag agctaggagc tcagactagc caggcaggat tggcaacagg
      181 gatttatatt ctagggactt tattagcccg tttgattttt ggaaagcagt tggaggtgtt
      241 tggtcgtcgt ttggttttaa gaggaggtgc tattttttac ctcttaacga ccttagccta
      301 cttttatatg ccaacgatta gcatgatgta cttggttcgt ttcttaaacg gatttggtta
      361 tggtgtggta tcaactgcga caaataccat tgtaacagcc tatattccag cttgtaaaag
      421 aggagaagga attaattttt atggcttgtc aaccagtcta gcagcagcta ttggtccttt
      481 tgtaggaacc tttatgttgg ataatcttca tattgatttt cgcatgatta ttgtattatg
      541 tagcgtcttg attgggtgtg tggttgttgg tgcttttgct tttccagtca aaaacatgtc
      601 tttaaatgcg gagcaattgg ctaaaaccaa atcgtggact gtggatagtt tcattgagaa
      661 aaaagccttg tttatcactg caatcgcctt tttaatgggg attgcctatg cttcagtttt
      721 aggatttcaa aaattataca cgtctgaaat tcatttgaca gcagtaggtg cttatttctt
      781 tgtggtctat gccttaatca ttaccatcac tcgtccagca atgggacgtt taatggatgc
      841 taaaggcgat aaatgggtct tgtacccaag ttatcttttt ttagcaatgg gactcttctt
      901 gctaggtagt gtatcgtctg gaggaagcta tttgctttca ggagccttga ttggctttgg
      961 ttatggcact tttatgtctt gtggtcaagc cgcctccata caaggagtgg atgagcatcg
     1021 ctttaatacg gctatgtcta cttatatgat tggtcttgat ttaggtttag gtgctgggcc
     1081 ttacctcttg ggcttgatta aagatttggc acttggtagt ggtgtggctt cctttaggca
     1141 cttattctgg ttagctgctg tgattccact gatttgtacc ttgctttact tgttaaaaac
     1201 aaagacaaga caggttgtgt cttaaggaca agaaaaggag tttagtctta tacttaaata
     1261 caaacgtatc ttaacagctg tagatagttc ctatgagccg ggattagctg tgggtaaagc
     1321 cattactgta gcgctttgaa ataatcctag tttgctattg gtacatgtca ataaaaatga
     1381 ctcgcaaaag ccattcgtga aagagaaaat gtggccttat ttttagtagg tgcaattgga
     1441 ttaacatcag aatataggat ttgtcatgtt aagattgatc tgttgatagt gagagctaat
     1501 aaaaaatgtt ataatactat gaaataaagt gataaagata aggtctttta tggtcctttt
     1561 ctttaccgct tttattattg tgtgaaaata acacttaatg atcattttat ttattatttt
     1621 attaccttta cattcatcaa taagcctgtt aaaatggtat ttgttatatt ttattatttg
     1681 gaggaaacca gaaaacatgg aaatgacatg gactgtgaaa tacatcacag aatttatcgc
     1741 aacggctttt ttgattattt tggggaatgg agctgttgct aacgtcgact taaaaggaac
     1801 aaaaggacat aactcaggtt ggttagtgat tgcttttggc tatggcttag gagttatgat
     1861 gccagctttg atgtttggta atgtatctgg aaaccatatt aatcctgctt tcacagttgg
     1921 actagcagta tcaggcttgt tcccatgggc tcacgtctta caatacgttg tggcacaatt
     1981 acttggagct atctttggtc agttggtagt ggtaatggtt tataaacctt actttatgaa
     2041 aactgaaaat ccaaatcatg ttttaggctc attctcaacg atttcatctc ttgataatgg
     2101 tcaaaaagac agccataaag tctcttatat caatggtttc ttaaacgaat ttgtaggttc
     2161 atttgttctt ttctttggtg ctttggcttt gactaaaaac tactttgggg tagagttagt
     2221 tggtaaattg attgaagcag gttacgacca aacaactgca gcaacacaaa tttcaccata
     2281 cgtgacaggc tcattggcgg ttgctcacat tggtattggt ttcttagtta tggtcttggt
     2341 tacttcatta ggtggaccaa ctgggccagc actaaaccca gcgcgtgatt ttgggcctcg
     2401 tttgttacac cacttcttac caaaatcagt tcttggtcaa gccaaaggtg attcaaaatg
     2461 gtggtatgca tgggttcctg tagttgcacc tatcttagcg gcgatagtag ccgtagcagc
     2521 gtttaaatac ctttatatca gataaaaaaa gaactaaagc ttcttttaga ctcaagacaa
     2581 gctcgcttaa ggaacttgtc ttttttatat gagttaagcg aaatctaatg caaacttgac
     2641 tgtcttgtat agcgtatttt gtaaatttta ctagacttct tgtaaaaaag cctctcctca
     2701 gataagatga ctaatgcctc taaacggtgt tacaaataaa cgaggcatcc ctaacttcct
     2761 ttatctggtg ttgaaactat tttgcacgga ctaaatcact gatagagtaa aaaacgattt
     2821 ttcatttttg ttgtaaaata ttaaaaacgt ctctggttag tgattcccag aaacgttatc
     2881 ttatctattt gtttgttgct tatgagaggt aaaggaggaa cgaatcagca ttccaccagt
     2941 gaagaaaacg atagcgatga ccagaagttt caagctatta atatctaaat tcgttaagaa
     3001 aaacacagct agtaaaacgc caataatacc accagaaaca attccagcaa aaccatccca
     3061 actgacacgg tttgctttga taaactggct actagaagct gtcataatca tagctgcatc
     3121 tagcatcata acaggcatag caactgttgg gctaagaccc attaatgaaa agaaaatcaa
     3181 ttctggagca taattgccta accccatagt catcaagact ccaacgataa aattaaaacc
     3241 aattccaaca aaaagccaga tgccatggag gccatgaatg gtgtctgaaa ggtgttctcc
     3301 tggatggtag attattctaa tgatcatgat tatagctgct atgattaaaa gactaccaag
     3361 gattcgttgg acagttggag catgccagtt ttttgtaata tgagtcccca aataagctcc
     3421 aataaaagct gctgcagcca tagcaagtaa agttagaacc tcaactttta cgacaaaaat
     3481 aaagcaaaga gattgaatga gcaccggaag cacatgagct acggtcatag tgccaggcaa
     3541 ttgtctatcg tcagtgacta atttagttaa cttaaagcac gttgtggtag tagcaaaaga
     3601 ccctatccca agggtgtcaa gggcatctgt tagcaggcca atccaaaacc ccgtccaaaa
     3661 gcgtttaaaa ggattgatct tatttttttt aagatgtttt actaacataa agaaaataaa
     3721 aagcattgcc gaaactagca aagcttgtat cagtctaagg ataagcattt cgttcataaa
     3781 gactagttta caaaaaaaaa ccttagccgt caacgtgatt tacttcatta aaacatgcca
     3841 ctatgcttgg aagagctttg gttcgatgta ttttgttaaa attaagctaa aatattaata
     3901 ttgcatgaaa ggggacgcct tcctttttcc cttactgagc ttagctgtta ttgtagtgaa
     3961 tgtttaaaag gctagtttgt cgatctttat caaaaatcta gtaggatgaa tacgtataat
     4021 ttcttgtcaa atagaggaat tttgtctata atagcagagg attaggagga aattatgact
     4081 ttaatggaaa atattacctt taaaactcca gtattacgcg ttaatgatcg cgaccttaat
     4141 attgcatttt atcaaaataa tttagggctt cgtttagtat ctgaagaaaa tgcgattgct
     4201 atcttttcat cttggggaga agggcaggaa tgttttgtaa ttgaagagtc accttcagtt
     4261 agaacgcgtg cggtagaagg ccctaaaaag gttaatacga ttgtcatcaa aacaaatcaa
     4321 cctaaagaaa tcgaacaatt attagctcat ggagcatatt atgatactct ttttaaaggt
     4381 caaaatggct atgcctttga gactatttcc ccagaaggag accgattctt gttgcatgct
     4441 gaacaagata ttaaacatct ccaaggtact gatttaccat cgcttgaaaa agatgcaact
     4501 tttaaaggtt taactcaatt taagtttgat attattgttt taaatgttat tagtgaagaa
     4561 cgctcaaaag ctttttatcg ggatctgttt agtgatcagt tacctattac tatggatttt
     4621 attcaagaag aaggtcccga tttggcaatt gatccccata ttgcttggga tttagaaatt
     4681 ttagaatttc aagtctcaaa ggactatgat atgaaggtcc taaaagcaac tttagaagaa
     4741 gacgggcaca aggtctacat tgataaaaaa cacaaagtat tggtcctttc tgatccaagt
     4801 cagatcgaag tatggtttac gaaatgactt tggcagtcat taaatgtatt gaaaaccatc
     4861 ttcacaaaaa agtatacaaa ggggccagtc tggctctctt tcaatctggt cgatggcaag
     4921 aataccatat cggaacaatt gatggaagac gaccagttga tgctaactta gtttatgact
     4981 tggctagtgt ttcaaaagtt gttggtgtgg ccacaatatg caatatttta ttgaataatg
     5041 gtacgttagc attagatgat cccttaaaag catattatcc tagtattgct gatgcgactg
     5101 tcactattcg acagttattg actcatacca gtggtcttga tccttatatt cctaacagag
     5161 atgttttaaa tgcacaacaa ttaagaaaag cacttaatca tcttactcaa aaagaaaata
     5221 aaaactttta ttacacagat gtcaatttcc tcttgttagg ctttatgtta gaagagctct
     5281 ttagtgaatc tttagaccag atatttgata agactatctt tactcctttt gggatgtatc
     5341 atacgtcttt tggccctcgt ccagaagctg taccaacatt aaagggtgtg agtgatggag
     5401 aagttcatga tcccaaagca aaaatcttaa aaaaacattc tggatctgcg gggctatttt
     5461 caactcttgc agatttagaa agttttagca atcattatct taatgatcca ttttcagatt
     5521 gtctgtggcg caactacagc caacaaacaa ttgagcggtc actagggtgg aatttagatg
     5581 gcgattggat tagtcataca ggttacacag ggccatttct aatgcttaat aaaaaagagc
     5641 aaacggcagc tatttttttg accaaccgta cttacgacga ggatgataaa agtaaatggt
     5701 taaaagaacg tcagttgctt tacaatgcac taaagcatga tctgacaacc cccgtatcgt
     5761 aacgtctaaa ggggcagttt tagtgatctt attactgttt ttaaagtttc ttctaaaaaa
     5821 tagccatttc atttgtctga tgcgattaga gtgattataa gtgaaaaaag aatagccatc
     5881 tcattttgaa tcaaatgaga tggctgttct tttttataag ttattgatca tttagatact
     5941 actatttact tgaaatagct tcagcagcat cgtccatgga aagcacttcg tggaagacac
     6001 gttgtgtcaa ctcagttttt tgttctggtg taaggtattt ggtgttgaca cagtaaccag
     6061 agatacgtac gataacgtct tcgccgttca tgattttgtc ataaacatcg ttaaggtcca
     6121 taacgttcaa gttaacgtgc tgaccaccat tttcaaagta accgtcaagc actgttacca
     6181 agttgtcaac ttgttcgtca aatgttttac caagagcacg aggtgatact tgagtagtca
     6241 atgagatacc atcgttagca tgtgagaatt ccagtttagc aagtgagtta aggttttgta
     6301 accagccacc ttttgctttg ttagatgggt tagcacctgg tgagaagaat tccacttggc
     6361 tagtgttaac tgtaccatct tcgttcaaga agacgccgcg gtggactgga gagttaccag
     6421 tttgtttaga gtaagcaacg tttgaagtga tggtaagaag tgatactgaa gcctcagcat
     6481 tcttgtaaag tttgtggctt gcaagacgtg tgtggtaagc ttccatcaac catttcgcga
     6541 tgtcatcaac acggtcgtca tcttcaccgt aacgagggaa gtcacctgtt acttcgtagt
     6601 catagatgta gccattttca tcgcggatag ttttaacagt tgcgtattta atagctgaca
     6661 aggtatcaac tgtgttagcg aaaccacaga taccgaatcc catgttagca cgttggtgtg
     6721 ttggcaagaa ggccatttga actgcttcgt agttgtattt atcagtcatg tagtggatga
     6781 tgttaagcgc atctacataa gtatctgtca accagtcaag agatttctca aagttagcca
     6841 taacttcgtc gtactcaaga acttctgatg tgattggttc aacaacgtta aacactttgt
     6901 agtctctgtg aacatcatcg taaccaccgt taagaccagt aagaagggct ttaagaacgt
     6961 ttacgcgagc accgaagtat tggatgttat gacgttgttc ttcattttct gggtcaagtg
     7021 gtgatacaca acatgaaata catgacatct caccataacc ttctttagcc attgttgtca
     7081 caccttcata ttggatagaa gagtgtttgt ggctcatttt catacagtag cgacggaatg
     7141 tttctggcag ttggtcagtc caaagaactg ttaagtttgg ttctggagag ttaccgatat
     7201 tatcaagagt gtttaagaaa cggtagtcca tcttagtcac acggtgacga ccgtcgttac
     7261 ccataccagc catagaagtt gtgatgaatg ttgggtcacc tgagtaaagg gcatcgtaag
     7321 ctttagtacg accaaatttt acggtacgga gtttaaggac aaaatcatca acaaattctt
     7381 ggatttctga ttctgtaaag gtaccgcgag caaggtcacg ttctgcaaag atatcaagaa
     7441 cgattggcac acgtccaaga gaagtagcag caccgttgat cacacgacat acagccatga
     7501 aagcgatgtt tacccattgg atagcttctt taacgttttg tgctgggcga cgaacatcaa
     7561 ctccgtaaag gtcaccaagt ttaaccactt caccaagagc ttggtattga aggttaactt
     7621 cttcgcgaag acgaatggat tcttcatcga tttcagtgat agcattccag tcattgactt
     7681 tttcttgcat caagtagtca gcaccgtaaa gagcaagacg agcgtaaaca ccgatgatac
     7741 gtccacgtga gtaagcatct ggaagaccag ttacagtgtg ggcatgacga gcgcgacgaa
     7801 tatttgaagt ataagcgcgg aagataccgt cgttaacagt tgtcacatac ttagtaaaga
     7861 tttcatgtac tgctgggtct ggttcatagc cgttttcttt aagggtcgtt tccgccatac
     7921 ggataccacc ttttggcatg aagttcaatt tgaacaattc atcgttttga ataccataaa
     7981 ttaattcatt ttctttatcg atgaaaccag caggaatgtc agcaattgaa gttggacgag
     8041 tatcgtatgg gaaacgtgta gcttcatagt gtgcttttgt ttcttcgata acttttttga
     8101 tgtgaagtga gcgttcagta gcaccagcca agaaagattc atcgccatcg tatggggtgt
     8161 agttagcttg tacaaaacgt gagacacttg ctttttcttt ccagtcagtt cctttaaagc
     8221 cttcccaagc tttttcgaaa acatctgtat tggttttaac agttgccata aatttctcct
     8281 taaattctag taacaaagcg atctgttaca tttacaattc taagtttaac acataggaaa
     8341 cgttttccca agcaaaattc ttgacaaaat taagattctt ttataataca gattgttgaa
     8401 acagtcacaa actgtattag aatgtatttt tttggcttta gatagcctaa aaggtataat
     8461 agagaaagga gggacttatg cttatttttc cactgattaa tgacacgtca cgaaaaatca
     8521 tccatattga catggatgcc ttttttgctg cagttgagga aagggataac cctgctttaa
     8581 aaggaaagcc tgttgtgatt gggaaagatc caagagaaac aggtggtcgc ggagttgttt
     8641 ccacttgtaa ttacgaagcg agaaaatatg gcattcattc ggccatgagc tctaaggaag
     8701 cttatgagcg ttgtcccaaa gccattttta tttcaggaaa ttatgaaaag tatcgaacag
     8761 ttggagacca gatccgccgt atttttaagc gttatactga tgtggtagag cccatgtcca
     8821 ttgatgaggc ctatctagat gtcaccaaca ataagttggg tataaaatca gccgtcaaaa
     8881 tagccaagct gattcagcat gatatctgga aagaagtagg attgacctgt tcagcaggtg
     8941 tgtcttataa caaatttttg gctaaattgg ctagtgattt tgaaaaaccc catggcctca
     9001 ccctagtctt gaaagaagat gccctgtgct ttttagccaa actccccatt gaaaagtttc
     9061 atggtgttgg taaaaaatca gttgaaaaac tgcatgacat ggggatttat acaggacagg
     9121 atttgttggc agttcctgaa atgaccttga ttgatcattt tggtcggttt ggttttgacc
     9181 tttaccgtaa agcgagaggc atcagcaatt ctcctgtcaa gtctgatcgg atacgcaagt
     9241 caattggcag tgagagaacc tacgctaaac tgctttatca agaaacagac atcaaggcag
     9301 agatcagtaa aaatgctaag cgcgtggccg ctctcttaca agaccataaa aagttaggca
     9361 agaccattgt gctcaaagtg cgttatgctg attttaccac cttgacaaaa cgtgtcacct
     9421 tgccagaatt aaccagaaat gccgcacaaa ttgagcaagt agctggggat atttttgaca
     9481 gcttaagcga aaatcctgct ggtatccgcc tgttaggggt aaccatgacc aatttggagg
     9541 ataaggtagc tgatatttcc ttggacctat aggagaaaga gtgctctatc cgtccgcctc
     9601 attttggaga aaaaagaagc tgattaggga tttccctaat cagcttcttt ggtccattat
     9661 tttattacag catagcttgg tctgtatcat cacctttttc gttaaggttt tttgctttgt
     9721 aaaaagcaaa gccataaaga gcagcttgta taaggataag gatagttcca accattgaca
     9781 aggttgttgt gaactggtta accaagccga taacagaaag gataagtcct aatatgtaag
     9841 gaatataact aaccgttaat ccttgtttta gtttggataa gttctgagta cagaagatga
     9901 taattgcaat aatagcaagg acatttaaga cagataagaa aatcataaat ggtgtcatgc
     9961 tgctttgtag ctgtgccaat tgctcagttg ttaattgggc ccgaaatgct tcattcttga
    10021 gttggctttg taagtagaaa atccctgcaa atcccattaa tgacagtaca actccaagcc
    10081 cgtttagaat aataatggca atagtagacg ttttgagtgc ctgacgcact ttttcataag
    10141 aaatcatata atcctcctag agtccttaat ccgaaattgt ataatgtgtt taatacaaaa
    10201 atattatacc atagattaat caaaaatgtc cgtttttcta ggattttttc tggaaaaaga
    10261 gggcaatatc tgactctgta agccctatca tcgaatcgat agtcgaataa ttctcaggtg
    10321 ttaacctaaa atcatcatta tctgactgag agttctcagt ggggttagag tttgttgatt
    10381 gagaggaaga tttttgagag tcgtcctcaa gggttgccgt attagaaaaa agggaggtct
    10441 gctcttttga ttttagctct tggttaggtt gagaggaggc taagtcggat tgttcttgga
    10501 agcgttcgat caagtaggtt tggcgtttat cgccttcgtg tttaatagca tactcaaagg
    10561 cagtatattc tcccaacaga attaacttac ttttagaccg agtaatggcc gtgtaaatca
    10621 catttcgttg caagagtcgg ccgctttggc gtgtgatagg taaaatcacc acttgaaact
    10681 cactcccttg cgatttatga atgctcatgg cataggccag ggttaatttt agccattcat
    10741 ttctagggta cgtgacttcg ctaccgtcaa aatctaagat taattcgtct tgcttagatt
    10801 cggtgtattt agcaggaatc aaatctgtaa tataaccaat atccccgtta aagacattca
    10861 actgagcatc gttgactaag tgcaggactt tatcaccttt acgaaaatgc gtatcattga
    10921 acagaaattc tgtttgtcct tgcaagggat tgagaagttc ttgcatgagt tgattaagat
    10981 gattgatgcc agcttgtcct ttgtacatag gtgctaaaat ttgaatttcc tcagcaggga
    11041 tgccactttt aatagcagat aggacaattt tttgaatcat gtcagggata aaggctgctt
    11101 gggcttcaaa ataagaacgg tcagcttttt tatcacgaaa gtcagcagct aagattcctc
    11161 gacgcatctg gtctgccaaa ttgacaatag tagattcttg ggattgacga aatatctttt
    11221 gcaaggcgat ttggggcagg ctattgactt ttaaaagatc tgacaggact tgaccgggtc
    11281 caaccgaagg aagctggtca ctatctccga caataatcac ttgagttgtt gagttaatcg
    11341 ctcctaacaa ctggttggca agccaggtat ccaccattga aaattcatcg acaatcagca
    11401 agtcgcagtc tagataatcc tccatggctt ggtaatcgtt gtcgccattg aggcctaagt
    11461 gcctgtggat ggttgcgcta ggaagtccag tcaactcatt catgcgacgt gcagctcgac
    11521 ctgttggagc tgctaacaag ataggaaggt cttttttatc caaatcaatc tgatgcaggt
    11581 tcgcgtaagc ctgcaaaata cctcgaataa cagttgtttt tcctgttccg ggaccacctg
    11641 ttaggagaaa gaccttgctg gttaaggcct tagtaatggc ttcttgctgc acttggtcat
    11701 aggtgatggc aaagtctttt tgaacggctt ggatggttgt ttgaatggta tcatgactaa
    11761 atgactgact taaaggagta tctaagagac gagagatgtt gttagcaatc ccttcttcag
    11821 caaaataaag gctagcatca aacagtttgg tgtcactgtt tttgattttg ccctctatga
    11881 tcaactcgct tagttgttca gccacagcag caggatcaca ttctacttga cgtgcatctt
    11941 caaggagtgt gatggcaaag tctaataatt gtcgtgcttg gacataagta tctcctcgat
    12001 tgatggattc ttccaagaga cagtgaagga gggctgcacg aaaacgtttc ggagagtcgc
    12061 tttcaatccc taagttttca gcgagagcat ctgccatttt aaacccaaaa ccttgtagat
    12121 cttcgactaa ctgataggga ttttctttaa caaggtcaag agcctcttct ttatactttt
    12181 caaacgcttg aagggcaaaa cggttgctaa ggccaagttc gacgagacca gcaatcagtt
    12241 gctctgtgcc gtaattcaat tttagtttag cgacaaaggc ttgacgatta gccttagata
    12301 ggccagagat agtttctaat ttgcttgggt cttctaaaat atggtcaatg gtattatgac
    12361 catatagcgc aatgattttc tccgctgttt ttttaccaat ccccttaaaa tggtcgctag
    12421 agaaataatt aaccaaacca gatgaactag gtttgatttt ttggtaacgg cttagtttga
    12481 gttgctgtcc atatttaggg tgctgggtca attcccccca aaaggtgtag tcatctcctt
    12541 caataatatc agccatcgtc cctgtgataa tgatttcaaa gtcgtcaata tcactatctg
    12601 tgtcttcgat ggcaaggagg agaattttaa aaaaattagc tgcattttca aagataatgc
    12661 ggtcaacagt accagtaaaa aagtgttcca taaggcacct ttctgtctat acaagatagt
    12721 cgaaaacctg cccaaaggca ggttttttct aattaaagac ggtcatttta ttgagtggcc
    12781 aaaaacgagc tttcacttca ccgataaggt tttctttttt gaaactacca acttcgcgac
    12841 tatccctgga aacaatacga tcatcaccaa gaagaaggta ttctcctttt ggaacactca
    12901 tctcaaagcg tgtttgtccc tcagaattag ttgtaaaagc atcagatgtt tctgctaact
    12961 gttggaatag ggtattatag gcgtaagttt tttggagttt atcgttttta aattgtttta
    13021 gatactcagc caaatacggc tcaactgttt ttttaccatt aatataaagt gtgtcatcat
    13081 tataagaaat ggtatcgcct ggcaatccaa caactctttt tacgatttct ttcttttgtc
    13141 cgttttcttc ttcctgagca actacaatat caaagcgatc aatcctagct tgattaaaaa
    13201 cgataaggcg ttcgccatga gctagagttg ggtccataga atggccgtct actttaacag
    13261 cctgccacaa aaaaagacga gatagaccaa agagaattaa aaagagagtg aatgggcccc
    13321 attctttaat aaactgtttc atatgattcc ttttatttag tgagaagcgc ttgcgctttg
    13381 tgagtgttgg cgaagtgaag tttagcacta tattctaagc tagacatacc ataagcggct
    13441 aagagttggc tagcaacttt atctgaagct gaaccagcac cgcttgggag ttggtaacct
    13501 aaatcttgac ctaattgatc gagattgtct aaaaatagat ttctagcaat gatagagcta
    13561 acagccacag ccaaatagtg actttctgcc ttttcttgaa aggttagagg atttgggaaa
    13621 tggctttttt ccttttttag atgcttttca taatttgatt ggctagtgaa agcatcaata
    13681 acaatttgtt taggttgaat gcctttttgt aacaacaaaa aaatagcttg gttatgaaga
    13741 gcaactttga tagaaatggc attatatggt ttacttttac caaccaattc gttatatttt
    13801 ttaggtgata ggagaagtga ttgatgaggt atttgtttct ctaacagagg cgcaatctgt
    13861 tgaatggttt tatccgataa ttttttagaa tcatcaaccc caagcttttt caggaaggaa
    13921 tgatctttag gatcgacaaa acttgccaca acagcaatcc caccaaaata agagccatta
    13981 cccacctcat cactgccgat tataggtata tcttggctat tgttgcttgc ttcaaaaact
    14041 gtcttagcta ctggaagatt aagctcttga gctaaagcat ttgcaccgtt tccctggaga
    14101 actaattttc cggatttgta cagcaaaaca gtaaccccat tttttttagc agcaaaggct
    14161 acataggtat tttggctatt gatggtgtag ggggctagct gtttttttaa atgtttactc
    14221 agtatagcat ctattttcaa aactaaggta ttcataataa ttattttaac ataaaatagt
    14281 tgactatggt taattcataa gtttatttaa caaagaaaac agagactcga atttttagac
    14341 aggttagggc aatggtgttg actattttac gaaagtttca ttttacggta taatgctagt
    14401 gaggtgaccc tatgaaaagt attaatcgct acaaattcac ctttggtgaa aaaacattaa
    14461 cattgacaac tgacaaagac aatctcttta tggaagaagt tgagcgggta gctaaagaaa
    14521 agtatcaggc tcttaaaaat catttaccag aagctgatga cgagacaatc gctattttaa
    14581 tggctatcaa taccttgtca acacaattaa gcagagagat tgccattgaa aaaatggaag
    14641 ctgaaatttt agatttacgt caaaaaacct tggttggtct tcaagaaaaa gctaatcaaa
    14701 tagatgtgaa tgaggtttaa tcatgttatc tttattgata gtgcttgttt taacttggaa
    14761 tttttatatt ggttatagta gaggtattat tcttcaatcg ttttatgtct taggagccct
    14821 actttctctt ttggtagcta atcgatttta tattggatta gcccataagc taacgctttg
    14881 gattccttat tctaacccgg tagaaggaac atcagtcttt ttctttaaat cagttgatat
    14941 ctttgtttta gataaggttt attatgcagg ccttgccttt tttatcattt tcttattggg
    15001 gtatgcactg agtcgctttc ttgggatttt tgttcatttc cttttgctag attattttga
    15061 taaccagtgg acaaaatgtt tgagtggcgg actagcgctt ctagtatcgc ttcttttttt
    15121 gaacatgtta ctgtctattt ttgcaacggt ccctatgcct tttttacagc actatttgca
    15181 ctctagtttt cttgctcgtt tggtgattga acaccttcca ccattgacaa taattattca
    15241 aaaattgtgg attcaagcca ttatttgata tcgttttgtg actaatataa gaacctggag
    15301 tgagtttcac ctttaggttc tttatttacg agaaactgtt tagaaataaa gagaacgcat
    15361 gggaacttag tgactttctt gccacatttt ccatcatttt aggtataatg aagagtatga
    15421 ataacaagat tttagagcag ttagaattta acaaagttaa ggaattgcta ttaccttatc
    15481 tcaagacaga acaatcacaa gaagaattat tagagctgga gccgatgacg gaggctccta
    15541 aaatagaaaa aagttttaat gaaatttctg acatggaaca gatttttgtt gaacatcact
    15601 catttggcat agtcagccta agttcaatct ctgagagttt aaaacgctta gagctttcag
    15661 ctgatcttaa tattcaagaa cttttggcta tcaaaaaagt tttacagagt tcttcggata
    15721 tgattcactt ttattctgat ttggataatg tttctttcca atctttggat cgtttgtttg
    15781 aaaatttgga acaattccct aatctgcaag ggtcttttca agctatcaat gatggtggtt
    15841 ttttagaaca ttttgcgagt ccaaaattag agcgtatccg tcgtcaatta acaaatagtg
    15901 aacgacgggt tcgtcagatt ttacaggata tgcttaagga aaaagcagag cttttatcag
    15961 agaatctaat cgctagtcgt agtggacgaa gtgtcctacc agtaaaaaat acttatcgga
    16021 atcgtatttc tggtgtggtt catgacatct cttcttcagg aagtactgtt tatattgagc
    16081 ctcgtgctgt agttacacta aacgaagaga taacgcagct tagagctgac gaacgccacg
    16141 aagaaggtcg tattttacac acattttcag acttgttaag acctcatgtc gccactatta
    16201 gaaataatgc atggattctt gggcatcttg attttgtaag ggctaaatat ctttttatgt
    16261 ctgataataa ggcaacgata cctaagatct ctgatgacag cacgttagca ttaatcaatg
    16321 ttcgtcatcc tcttttaagt aatcctgtgg ctaatgactt acattttgat cacgatttaa
    16381 ctgcaattgt catcactggt cccaatactg gtggtaagac gattatgcta aaaacactcg
    16441 gtttagcaca attaatggga cagtctggtt tgccagtatt agcggataaa ggtagtaaaa
    16501 ttgcagtatt taacaatatc tttgcagata ttggcgatga gcaatctatt gaacaaagtc
    16561 tatcaacttt ttctagtcat atgacgcaca tagtcagtat tttaaacgag gctgaccgca
    16621 atagtttagt tctctttgat gaactaggag caggaacgga tcctcaagaa ggtgctagtt
    16681 tggctatggc tattttagaa caccttaggt taagtcatat caaaacgatg gcgaccacgc
    16741 actatccaga attaaaagct tatgggattg aaacaaattt tgtagagaat gcgagcatgg
    16801 aatttgatgc agaaacgctt agtcctacgt atcgctttat gcaaggagtt cctggacgct
    16861 caaatgcatt tgaaattgct tctcgccttg gtttagctcc atttattgtt aaacaagcta
    16921 agcagatgac agattctgac tcagatgtta accgtattat tgaacagtta gaggcacaga
    16981 cacttgagac acgtagaaga ctggatcata ttaaagaagt tgaacaagaa aatctcaaat
    17041 tcaatcgtgc ggttaagaaa ctctataatg aattttcaca tgaacgcgat aaagagttag
    17101 aaaaaatcta tcaagaagct caagaaattg tagatatggc tttgaatgag agtgatacta
    17161 tcttaaaaaa actcaatgat aagagccaat taaaacctca cgaaattata gatgctaagg
    17221 cacaaataaa aaaattagca cctcaagttg atttatcaaa aaataaagtc ttaaataagg
    17281 ctaaaaaaat caaagcagct cgtgccccta gaattggtga tgatattata gtgactagtt
    17341 atggacagcg aggtacctta actagtcaat taaaagatgg ccgttgggaa gcacaagtgg
    17401 gaattatcaa aatgacatta acacaagatg aatttagcct cgttagagtt caagaagaac
    17461 agaaagtcaa aaataaacag attaatgtgg ttaaaaaggc tgatagttct ggaccaagag
    17521 ctcgacttga tcttagaggt aaaagatacg aagcagcaat gcaagagtta gatcatttta
    17581 ttgatcaagc attgcttaac aatatgggac aagttgatat cattcatggt attggtacag
    17641 gcgttattcg tgagggagtg acaaaatatc ttcgtcgtaa taagcatgtt aagcattttg
    17701 cttatgcccc acaaaatgca gggggatctg gtgccacaat tgtaacgtta gggtaaatga
    17761 tggaatcata tgaacacttt tatgccaaac tatctcaacc ttttagaaaa accccacagt
    17821 taataattct tttaaatgtt ttactaaaaa taatcacagg agtgatgtac atactttatc
    17881 catcttttct gatatttacg ctttggcaag ggatgacatt tcaattatgg ttaaggctat
    17941 taattattcc tgctgttggc tttatagctt tgtcttacat tagaaaacgc tttgattttc
    18001 cacgaccgta tgaaaaatgg aatattaaac ctttaattga taaggataca aagggaaggt
    18061 caatgcctag cagacatgtt ttttcagcta cgatgattag tatgtgtttg ttacgatatt
    18121 atgtttactt tggaatagtt tgtttaatat tatcggtttt attggcaatt tgccgtgtga
    18181 tagcaggtat tcactatcct aaggatgtta ttgtaggtta ccttattggc ttgatgttag
    18241 ggctttgttt attgatttag cgagttcgta gcaaatagtt tcaaaaacaa ctaaattctt
    18301 gtacaatagg cttatcatta agatgaaatg gagaaaaaag atggcattag aagtaacaga
    18361 tgctacattt gtagaagaaa ctaaagaagg tttagtactc attgattttt gggcaacttg
    18421 gtgtggtcca tgtcgtatgc aagcaccaat cttagaacag ttatctcaag aaatcgatga
    18481 agatgaactc aaaattttga aaatggatgt tgatgaaaat ccggaaactg ctcgtcagtt
    18541 tggtattatg tcaattccaa cattgatgtt aaaaaaagac ggagaagtgg ttaaacaagt
    18601 tgcaggtgtt catactaaag accaactgaa agctattatt gctgaattga gttaactaaa
    18661 ataaagccca gcgtcacgct gagctttatt ttatgaccta ctaacttcaa tggaataacc
    18721 atgtgaacat aaaacgactt gcaaaaacaa taacaacagt aatgaggaaa aatacagtga
    18781 caataagggg tttctgataa ggaagttttg ctctttcttc tcgtttttcg atttcgtcta
    18841 ataattttcc atttgaaaca gctaggattt ccttataagg ttgcacatca tattttttct
    18901 taaaacgttc cacacgatta gcataagtca tagtaattat cgataataaa acaaaaatgc
    18961 caagtcctag ccactctaag aaatagacta atggataact tgaaatcatt aatagtatca
    19021 tcaatgttac cattactttg gtatctcgtt caaaattctt tttatcaact tgagtgatgg
    19081 tatatttcat tttctcaatg tctcctttaa tgagttggtc taagctcact tgaaaaattt
    19141 gacttaggag taagaggcta tggatgtctg ggtaagtttt gtcattttcc cagttagata
    19201 tgctctgacg gcttacatag actttctcga ctaattcttc ttgcgagagc gtttgttgtt
    19261 tacggtagcg aatcatttgc tgaccaattt ccataaatat ctcctttcat aaaattagta
    19321 taagaatgat aagctaacgg gtctatcaaa tgtatttgac attagggata gatcactatt
    19381 tataaggtat taaaagacaa tctgaggaaa actttacagc ggcgtaaagg aattgtaaac
    19441 aaaaagtgaa ccgtcatagt tcacttgtga ttaagcattt ttctgtttta agtaggtctc
    19501 tagcattttc ttttgtggtg ttgcaaaggg atacagagaa aagtcctcaa ttgctaccca
    19561 ctttaggtga ggagcattag gtaaatctgt tgctttgacc accccctcta tgagttctat
    19621 tgtccacttc tggtgactaa aagtgtgctt aatatttgga aagtgattat cagtccattg
    19681 gggctttaat tgatattctc tttcgaaagt ttcattttgg gtttgccaga ttatggggtt
    19741 ggattgattg tcatcaaata aatctaattg ttgagaaaga ggtgacgttt cgataatagg
    19801 aaatgaccaa aaacctccta gaagtctccc tttagtattt ttctccagta gatattggcc
    19861 attttggtta cgaatgacaa aagcctgaat tcgcattggt ttaggctttt tctttggatt
    19921 ttttataggg tatttaccat aagtaccatt caaataagca gcgttaaaga aacgaatagg
    19981 gctttcatca ggtctaggtg tcttagcgga ttcgatgtct gtacctagat ccataagtgc
    20041 ttggttaaaa tcaccgggtc tgtcagggtc aattagtatt tccataatag cttgaaaaat
    20101 tttacgattt ttagggtcac cgatatcata attaacttca aacaaacgag ccatgacacg
    20161 catcacattg ccatcaacag caggctctgg taagttgaat gagatgctgg cgatagcacc
    20221 tgcagtataa ggaccaatcc cttttaatga agcaatatca tcataagtgt gaggaaatat
    20281 gccaccaaaa tcaaccatca cttgctgtgc agccttttgc atattacgca cccgagaata
    20341 atagcctagt ccctcccatg cctttagtaa ttgttcttca ggagcgtctg ccaaatcttt
    20401 tatttgagga aaccactcta gaaaacgttt ataataaggg attaccgtat taacttgtgt
    20461 ttgttgcaac atgatttcag aaacccaaat atagtatgga ttagtagttc ttcgccaagg
    20521 gagatcacgt ttttcttggt cataccaccc taaaagtgtg cgacgaaaag aagcgattgt
    20581 ctcattatcc cacatattaa taccataatc ttttaattca atcatgttgt tattgtaaca
    20641 caggagtctt aaaaagagta aaaaaagatt ttaggtattt gcgaaaaaga aaaggaaata
    20701 ttataataat attaggttta cctcatctaa atgttgattt agaataactg aaattaacga
    20761 tagaagcaga caggttttaa tttggagggg acatgttcca gaaattaaag aatcttattg
    20821 atcggtattc gtggttcgtt tttataatga tgattcttgt gttatttctt aactatattt
    20881 atccatttga tccttctttg agcggactac agttggaatg gctgatcatg acgcaagttt
    20941 ttcctggatt aggtatcgta tgtgccatgg ttgctattat ttttaaaaag tttaaccttt
    21001 ttgttttggc cctaatttta tattttgcac tttggttaaa acttttcctt gctttttctt
    21061 ttttcaccta ttttttagga gtctgactaa gtgccaattt ttaaaactcg tgaaaggaaa
    21121 tgtttgacaa aacttagtca agtagaaaaa gttccttgct tttaattgac tattagtgta
    21181 caataagatt ctgtgagtat acctcactta ctcactccaa aagtagtgag tcattagacc
    21241 aaaaggagga aaatatcaat ggctaaatac gaaattcttt atatcattcg tccaaacatt
    21301 gaagaagaag ctaaaaacgc tttggtagca cgctttgact ctatcttgac tgacaacggt
    21361 gcaactgttg ttgaatcaaa agattgggaa aaacgtcgtc ttgcatacga aatcaacgat
    21421 ttccgtgaag ggctttacca catcgttaac cttgaagcga ctgacgcagc agctcttaac
    21481 gagtttgacc gtctttcaaa aatcaatggt gacattcttc gtcacatgat cgttaaactt
    21541 gacgcttaat tccaactaag agggtagttt atgattaata atgtagtact agttggtcgt
    21601 atgaccaagg atgcagaact tcgttacaca ccaagtcagg tagctgtggc taccttcaca
    21661 cttgctgtta accgtacctt taaaagccaa aatggtgaac gcgaggcaga tttcattaac
    21721 tgtgtgatct ggcgtcaacc ggctgaaaat ttagcgaact gggctaaaaa aggtgccttg
    21781 atcggagtta cgggtcgtat tcagacacgt aactacgaaa accaacaagg acaacgtgtc
    21841 tatgtaacag aagttgttgc agataatttc caaatgttgg aaagtcgtgc tacacgtgaa
    21901 ggtggctcaa ctggctcatt taatggtggt tttaacaata acacttcatc atcaaacagt
    21961 tactcagcgc ctgcacaaca aacgcctaac tttggaagag atgatagccc atttgggaac
    22021 tcaaacccga tggatatctc agatgacgat cttccattct agtcagaatg gattgaacga
    22081 atacgaaatt atttgagttc gggctaaaaa tttagaactt agaatttaga tcaactccct
    22141 aggaaatcaa gatttcttgc cagttttcta aaattcagtc ttttttaaga gcccttaaca
    22201 tcataattta taaaggagaa aatacatggc tcaacaacgt cgtggcggat tcaaacgccg
    22261 taaaaaagtt gatttcatcg cagctaataa aattgaatat gttgattaca aagatactga
    22321 gcttcttagc cgtttcgttt cagaacgtgg gaaaattctt cctcgtcgtg taacaggaac
    22381 ttcagctaaa aaccaacgta aagtaacaac agcaatcaaa cgtgctcgcg ttatggctct
    22441 tatgccttac gtaaacgaag actaataaac caacagtcca tttcggactg ttttttagtt
    22501 gattagaata gtctatgcat gtagatgaat tttgtctaaa ataaagatga gagtaaacga
    22561 aaatccttgc gtagacagca aggatgaaat gattgttatt tgttaacagg tgacatagtg
    22621 ttttgaatgt tgtatttgcg ttgtaggtaa tagcgtaaag caagggaaac accaccgata
    22681 atgagcaaga caagaggagg caactgtgga ttaagagagg ttggtaaaaa ggcagttgca
    22741 gagtacaagg caatccaaat caacatagct aatgataagg ctgcgatgat tttgaaccaa
    22801 cttggacgaa ggctcttatc ttttccgaga tgacggtaaa taaagtaata agtagcgtac
    22861 atggatgctc ctccaccaaa accaagtgcc agtaaggaaa tcaatcctgt aactgtagcg
    22921 tttgtgttga agaaggtcat gatgccattt aaaagggcaa caattccgat gaatagcaaa
    22981 gaggtatcta gccacatcaa ccaaggattg gtgtttttgt cagtttctgc tgctctttga
    23041 ctagggtctt ggctaaaaga agctgcccaa gcagtcggag tgcctaatag atttcgagcg
    23101 gtgacacctt ttttctggtt ttctagaata gctggaatgg cttcttcgag aatcagctta
    23161 atatcctcat cagacttgcc gtcttggatt aatttgttcg tagcggtgtg gataaattct
    23221 tgattttttt tggtaagttc ttgtaattcc atgggaatcc cttctttata tgggttagtt
    23281 aaagtaagtc aatggtatta gctagttgta atgaaacttt ggagctagcc gtttgacaca
    23341 gagttttgtc aggtcttaac cgcattctgc agcttgctcc actctagaac catttttttc
    23401 taataaagta aatcacaaca aaggaactaa gtagcatagc aatcaaggta atataccaaa
    23461 aagcatgttc taggccattt agtggtaacc agttgttttg gaaattcata ccataggcgg
    23521 agaaaatcac agttggtata tctaaagcca tggtcatcaa ggccagtgtt ttcataatgg
    23581 tgttctggtt gttgttgata atagaagcag tggtttcagt catggcattc aagacatttt
    23641 cataaatgcc agccatctca atggcctgtt gggtttcaat caaggtatct tctagcaaat
    23701 cctcgtcctc aatgtatttc ttgagagagc ttgttgagct ggataatttc ttgacgatgc
    23761 gttcgttgaa tttcaaggaa gcctttaagt agacaatgga tttttcaagt tccatcatgt
    23821 cgataagttc ttcatttcga gtagctgctt caagctgagc ttccaaacgt tcgctttggc
    23881 gatcaatggt cctcaaggca gtcagaaaga gctcggcgtt gcgatataga atctgaaaaa
    23941 caaaacgtgt cttcatgaag gtatagaaat ttttgacccg gcggttatga aaatgatcga
    24001 aaagggtcat gtcatgcaga caagttgtga taacagcatt ttccgttaca ataatgccta
    24061 aaggcatagt aatatagtaa cttttattgt tacgttcttc gtatatcggg acgtctacga
    24121 taattagggt ataatcatct tctacggcaa tacgtgaggt ttcttccacg tccaaagggg
    24181 cgcgcaagtc agaaatatca atgttaaatt gatcggcgat cttcatagac tcttcttggg
    24241 aagggttaac cagtttaatc caagctcctg gctcgaatgt ttctatttct ttgaattcaa
    24301 ttgccgatga aagaaacatt tgtttcatga caagccctcc tttttgatga ttgactggta
    24361 gagattgaca tcaaaccttg ataataaagc cttctcaagg attttctgaa aaattctcaa
    24421 tacttatcta ttataccaaa attcgcaaag tttggggcaa taggttatca gaaatttttg
    24481 ttataatata agaaagctcg atgaggaggg tgcctttagg cacattcaag atgcaaaata
    24541 aaataataat tcatggggca agagcccaca atttaaaaaa tattgacgtg gaaattccac
    24601 gagacaaatt agttgttgtg acagggttat cagggtcagg aaaatctagt ctagcctttg
    24661 atactattta tgcggaaggg caacgtcgtt acgtagaaag tttatcagct tatgctagac
    24721 agttcttggg taatatggaa aaaccagatg tggattccat tgacggtctt agccctgcta
    24781 tttccattga tcaaaaaact accagtaaga acccccgctc aacggttggt acggtgactg
    24841 aaatcaatga ttatttacgt ttactatatg cgcgtgtggg cacaccttac tgtattaatg
    24901 ggcatggggc cattacagca tcttctgcag aacaaattgt agagcaggtc ttagccctcc
    24961 ctgaacgaac acgcatgcag attttagcgc ctattgttcg ccggaaaaaa ggtcaacaca
    25021 aaaccatttt tgagaaaatt caaaaagatg gttatgtccg tgtgcgggtt gatggggata
    25081 tttttgacgt gacagaagtg ccggagctgt caaaaagtaa gatgcacaac atcgaggtgg
    25141 ttattgatcg tctggttaat aaggatggta ttcgtagccg tttatttgat tcggttgaag
    25201 cagctttaag gctgggagat ggctacctta tgattgacac tatggatggc aatgaactgc
    25261 tcttttctga gcattattcc tgtcctgtct gtgggtttac ggttcctgaa ttagaaccaa
    25321 gactcttctc ttttaatgct ccgttcggct cttgtccgac ctgtgatggg ctaggcatta
    25381 agttggaagt ggatttggac ttggttgttc ctgacccaag taagtctttg aaagagggag
    25441 ctttggcgcc ttggaatcct atttcctcca attattaccc aaccatgctt gagcaagcta
    25501 tggcaagttt tggggtggat atggatacac cttttgaggc cttgactgag gaagaaaggg
    25561 atttagtgct ttatggttca ggtgatagag aatttcattt tcactatgtg aatgattttg
    25621 gtggtgagcg taacattgac attccttttg aaggtgttgt gactaatgtt aatcgccgtt
    25681 atcacgaaac taatagtgat tatacgcgca atgtgatgcg tggctacatg aatgaattga
    25741 cttgtgcgac ttgtcatggt taccgtttga atgaccaagc cttgtgtgtg catgtgggtg
    25801 gcgaagaagg acctcatatt ggtcaaattt cagaattatc gattgccgac cacttgcagt
    25861 tgttggaaga actggaactc acagaaaatg aaagtaccat tgctaaaccg attgtcaaag
    25921 aaattcatga ccgcctgacc ttcttaaata atgtgggact gaattatctg accttatcac
    25981 gtgctgctgg gactttatca ggtggggaaa gtcaacgaat caggttagca acacaaattg
    26041 gttctaactt atcaggggtg ctctatatct tggatgagcc ttctattgga ttgcaccaaa
    26101 gagacaacga ccgtttgatc gaaagcctga aaaaaatgcg tgatttgggt aatacgttaa
    26161 ttgtggtaga acacgatgaa gataccatga tgcaggcaga ctggctgatt gatgtggggc
    26221 ctggggcagg tgagttcggt ggagaaatta ttgcctctgg aacgccgaaa caagtggcta
    26281 agaataaaaa atccatcacg ggtcaatatt tgtctggtaa aaaattcatt ccagtgccat
    26341 tagagcgtcg ttctggtaat ggccgattca ttgaaataaa aggagcagcg cagaataatc
    26401 ttcaaagcct cgacgtgaga ttcccactgg gtaaatttat tgcggtcaca ggggtatcag
    26461 gctcagggaa atcaaccttg gttaatagta ttttgaaaaa ggctgtcgcc caaaaattaa
    26521 accgtaatgc tgataagcct ggtaaatatc actctatttc tggtattgaa catattgaac
    26581 gcttgattga cattgaccaa agtccgattg gaaggacgcc acggtcaaat ccagccacct
    26641 atacgggtgt ctttgatgat attcgagact tgtttgccca aaccaacgaa gctaagattc
    26701 atggctacaa aaaaggccgt ttctctttta atgtcaaagg tgggcgttgt gaggcttgtt
    26761 caggtgatgg gattatcaaa atcgaaatgc actttttgcc agatgtttat gtgccttgtg
    26821 aggtttgcca cggcagacgg tataattctg agacgcttga ggtgcattat aaagaaaaaa
    26881 atattgctga agtgcttgac atgactgtcg atgatgcctt ggtatttttc tctgctatcc
    26941 ctaaaattgc ccgaaaaatt caaaccatta aggatgttgg tttgggttat gtgaccttgg
    27001 gtcaaccagc tactactcta tctggaggag aagcgcagcg gatgaaatta gctagtgaat
    27061 tgcacaaacg ctcgacaggt aagagcttgt atatcctaga tgagccaacg actggtttac
    27121 acacagatga tattgcaagg ctattaaaag ttttggaacg ctttgtagac gatggtaata
    27181 cggttcttgt gattgagcat aatttggacg ttatcaaatc tgctgaccat attattgact
    27241 taggaccaga aggtggtgtc ggaggtggtc agattgttgc cacggggaca ccagaagagg
    27301 tagctcaagt taaagaaagc tatactggtc attatttgaa agtaaaacta caacaatagg
    27361 attaggagtt cgtttgagct cctttttaat cttttaaatg ctctcaaatc atggtaaact
    27421 taaagtagtt ttttcatgct acaatattct caaaagttac caaaggagtt cgcgatgtca
    27481 ggatttttag agcaacgatt aggtcactgc ctaaggcaga tggcagagaa ggggctagag
    27541 gctcttctgg tcacccattt aaccaatagt tattacttga caggtttttc tggaactgca
    27601 gcaactgttt tgataacggc caaacgtcat gttttgatca cagattcacg ttataccttg
    27661 cttgctaaag ctagtgttga gggatttgat attatcgaaa gccgcacgcc gcttaaggtt
    27721 gtggcagaat tgttagaggc tgatcaaata gattgccttg gttttgagga ccaggtatcg
    27781 ttttcttttt atcaggccat gcaagcagaa ctgtcaggaa taaccttgct tgctcagtca
    27841 ggttttgtgg agcatttacg tcttattaag gatacttctg aaattgatac cattgctaaa
    27901 gcgtgctcga tctcagacaa agcatttgaa gatgctcttg attttattaa accagggaca
    27961 accactgaac gtgacctggc taatttttta gattttcgta tgcgtcagta tggtgccagc
    28021 ggcacatcat ttgatatcat tgtagcttca ggctatcgct ctgccatgcc tcatggacgc
    28081 gccagtgaca aggttatcca gaataaagag agcttgacca tggactttgg gtgttactac
    28141 aatcactatg ttagtgatat gacgaggacc attcatattg gccaagttac tgatgaagaa
    28201 cgtgagattt atgctcttgt tcttgctgct aataaggctt taattgctaa agctagcgct
    28261 ggcatgactt atagtgactt tgacggtatt ccgcgccaac tcatcgctga ggcgggttat
    28321 ggcagtcgct tcacacatgg cattggtcat ggtatcgggc ttgacatcca tgagaatcca
    28381 ttttttggga aatctgagca acttctccaa gctggaatgg tggtaacaga tgagccaggt
    28441 atctatttgg ataacaaata tggtgtccgt atcgaagacg acttggttat cacaaaaact
    28501 ggttgtcaag tcttgacctt ggcacccaaa gaattaattg tattgtaaaa gaatggttca
    28561 ggaaaggatt aggggagaag caatgacaaa tcgtttatca tggcaagact attttatggc
    28621 caatgcagag ctgatttcca agcgctctac ctgtgatcgc gcttttgtag gagctgtttt
    28681 ggtaaaggat aaccgcatta ttgccacagg ttacaatggc ggtgtttctg ctacggataa
    28741 ctgcaacgaa gcgggtcatt atatggaaga cggtcattgt attcgcactg ttcatgctga
    28801 aatgaatgct ttgattcaat gtgcgaaaga aggaatttca acggacggaa ctgaaattta
    28861 cgtgactcat tttccttgta ttaactgcac taaggcgctt ttgcaggcgg ggattacaaa
    28921 gattacctat aaagcacatt accgtccgca cccctttgcc attgaactga tggagaaaaa
    28981 aggggttgcc tatgtgcaac atgatgtgcc tcaaattgtt ttaggaggcg ataaggaaca
    29041 atagaaagtg tacctaaaag gacggtcata aaagatttga gccaaggcca cttttgtggt
    29101 aaaatgaagt gataaatatt ttagtaagag gtaataataa tgattgaagc aagtaagctt
    29161 aaagcaggta tgacatttga agcagaagga aaattaatcc gtgtccttga agctagccac
    29221 cacaaaccag gtaaaggaaa cactatcatg cgtatgaaac tacgtgatgt gcgtacaggt
    29281 tctacttttg acacaactta ccgcccagat gaaaaatttg agcaagccat cattgaaact
    29341 gtcccagcac aatacctata caaaatggat gacactgctt acttcatgaa cactgatact
    29401 tatgatcagt acgaaattcc agttgctaac gttgagcaag aattgcttta cattcttgaa
    29461 aactcagacg tgaaaatcca attttatgga agtgaagtga ttggggtaac ggttccaaca
    29521 actgttgaat tgaccgttgc ggaaacacaa ccatctatta aaggagcgac agtgacgggt
    29581 tcagggaaac ctgcaactct tgagacagga cttgttgtta acgttccaga ctttatcgaa
    29641 gctggccaaa aactaatcat taacactgca gaaggtactt acgtttctcg tgcttaatcc
    29701 tttgtcaaaa cagattagtc taaaaaagag aaaggaaatg ctatgacaac tgaatatatc
    29761 ggtgaaatcg tcatttcacc acgagtgctt gaggttatta cggggattgc tacgacacaa
    29821 gttgagggcg tccactcact ccataacaag aaaatggcag atagctttaa caaagcaagt
    29881 cttggtaaag gggtttatct ccagacagaa gaagatggaa gtgttacagc agatatttat
    29941 gtttacttgc aatatggagt taaggtcccg actgtatcta tgaatattca aaaaacagtc
    30001 aaatctgctg tgtacgatat ggcagaagtg cctatttctg cagttaatat ccatgttgaa
    30061 ggtattgttg ctgaaaagac accaaaacca gatttgaagt cactttttga tgaggatttc
    30121 ttggatgact aacagctttc aaaattcaag aagagatttg cgtgaacgtg cctttcaggc
    30181 tttatttaac atagagatgg gagctgagtt attggcagct tctcagtttg cctatggtta
    30241 tgataaagta acaggggagg acgctcaagt attggagctg ccgatttttt tattaagtct
    30301 tgtgatgggt gtcaataatc ataaagaaga gttggataac ttgatatcaa ctcatttgaa
    30361 aaaaggttgg tcgttagaac gcttgacact aacagacaaa acgttattac ggctaggtct
    30421 ttttgaaatc aagtattttg acgaaacacc agatcgtgtt gcactaaacg aaatcattga
    30481 agtagctaaa aaatattctg atgaaacctc tgctaaattt attaatggct tacttagcca
    30541 atatgtctcg gaagctcctt cagcaaataa atcataataa gtcggagcaa tcttaggatt
    30601 gtatccgatt ttttcaattt atgtacgaat attaaaagac aaacaagcat actctcgcat
    30661 ctagaaagag tatgcttgtt tgtcttttag agtaattagt gattggttat gttttgttta
    30721 aatacttgct cctggaagta gcgtaatcgg taaaatatag tctttactgg ttttttcttt
    30781 tttaattttt ttaatgagta tttcgacact caatttagca atctcatcaa taggttgacg
    30841 aatggtcgct aattgaggaa catattgctg gataaatgtc gttccatcat aacctatgac
    30901 tttcatatcc tctggaatag taatgtgaag ttgtttggct actttcataa tgaggatagc
    30961 agttaaatca tcagaaacga aaaggccatc aggttttcgg gttgccaaaa tggatttaat
    31021 ttccatttcg cgtctaacag gagataggtt atttggtaat ttgatgattt ctgcacttcg
    31081 tttgagctgg tagttaaacc ctagctggcg caaccctgtg ggcgaatcag aattgtcatt
    31141 acctgtaatc atgacaatgt tttgacaacc gtgtttttgc aaagtttgtg cggctaattt
    31201 accaccttca aaattatctg aagaaataac cgggatattt ggcgccagat tacggtcaaa
    31261 agctacaata ggagcttcga cacgctcata atcctcaatt cccaaattat gactggaaga
    31321 aataatgccg tccacttgat tggccgcaag catttctaag tattcgcgtt ctttgacggg
    31381 attatgttca ctattgcaaa taatagtttt gtagccttgt ttaaacaatt caatttccaa
    31441 atgttcaatt aattcagcgt aaaagatatt gctaatgttg gggaagataa gcccaatgag
    31501 ttgggttgat ttaccttgca aacttctagc gagattgtta ggcttgtagc ccaattcacg
    31561 catggcctta ttcaccttat tcactgtttt ttgggaaagg tagccttttt tgttgattac
    31621 tcgcgaaaca gttgttggac tgacacccgc caaggctgcc acgtctgtta attttgcgac
    31681 cacttgccta atacctcatt tcataataat gtccaaaagc ttgtccttct actacttgga
    31741 tacctgtttg tccttgggtt gggaaaacac gactagttag gactttttgc ccttggttaa
    31801 taaagatttc aagaatggaa ttgtccacat agacattaag tgtaacatgg ctttgtggga
    31861 tttggcaaga gcgcaccgta ccatagtctt gggcatattg aacccctgcc cgacttcggt
    31921 caatgcttaa ctgtcctttt gttgtatcca ctttcaatct taggccattc cctttttggt
    31981 cggcaaatag gagcaggtca agcttttttt ggcctggaat cgtcaattcc aattcataac
    32041 agttagaact gtctatttta ttatgaaata attctgctgg ccctcgaaga ttttgtaggg
    32101 ctgaaacagg tgtttgatag agaattccgt ctttaatcct taattctttc actaaactaa
    32161 gagctccttg atagtcataa cgatctgtcg gataatcaat atctggtagc ccaatccaag
    32221 aaacggctag aaccctgcca tctggactac taaaggcttg agtggcataa gcttcaaaac
    32281 caaagtcaag attttgaagg gcccccccac ccagcaattg gccagtttct ggattgaagc
    32341 tttcaaaaat tttataggta ttggggtaga tattttgata atctaaatca gctttagcca
    32401 gtccttgtgg gctgaaaatc aaaacaggct tgtcatcgac aaacacaagg ttggggcatt
    32461 caatcatgta ttccgtacca gaatcgtcaa agtccaaatc gtctataaat cgccagttgt
    32521 ccacatgatt gtccacagct ttgtaaagtt tgatttttcc tttgccatct aaaccttggg
    32581 cgccaataat agcatagaat tgaccttgat aagaaaaaag ctgaggatcc cgaaaatgtt
    32641 ctgtaacatc atcgggctgt tcaatcaaaa cctgcgggat tttggaaata tttccttgtt
    32701 tatccatcca tgcgccaacc tgtaatggtg ttctgaccca gttggcatcg cgaacattac
    32761 cagtgtaaaa gaggaagagc ttgtcatcaa tagcataggc agagcctgag taagccccat
    32821 gactatcgtg agcgtggtct ggcagaaggc ggctccttgt ttccgtaaaa tggacgaggt
    32881 ctgtggatgt catgtgtacc cattgtttca gaccatgcgc agccccataa ggccagtttt
    32941 gataaaaaag atggtagcga ccgttgaaat aagaaaaccc attggggtca ttgagtaatc
    33001 ctgtcttagg ttcgacatga aattggctgt gccatgggga ttgggccatt ttttcagtaa
    33061 tagcttggta gtcttttgag ctccactctt tgtaagggcg gtagcgaata gcttgtggta
    33121 gatccatcaa aatctccttg ttttatagtc gtcttcaaga atatactaaa cgttttctgt
    33181 ttaaaaatca agaatttaaa gcatataaaa taaaaatatg ttaaacgctt gacataaaaa
    33241 atattagtga taaaatgata gtcaagaagt gaagcggatt cactagcgtt ggtcctacac
    33301 attcttagcc acaaagctgg cagaatggat gagggactaa caccaaatga aaaaggagac
    33361 ttttaggcat ggataatcgt cagattgcaa ctgaagtgat tgaggcttta ggtggccgag
    33421 aaaatgtgag aagtgttgcc cactgtgcaa ctcgccttcg cgtgatggtt tatgatgaag
    33481 gaaagattga taaggaaaaa gcagaagcta ttgacaaggt taaaggagct ttctttaact
    33541 ctggtcaata tcaaatgatt tttggaactg gtaccgtcaa taacatttat gacgaagtcg
    33601 ttgctcttgg tttaccaaca tcatcaacca gtgagcaaaa agcagaagca ggcaaacaag
    33661 gcaatatctt ccaacgggcg attcgtacgt ttggagatgt ctttgttccc attattccag
    33721 ctattgtagc aacgggtctc tttatggggg ttcgtggttt ggtgacccag ccagctatta
    33781 tggatttatt tggggtgcat gagcacgggg aaaattttct catgtacact cgtattctaa
    33841 cggatacagc ctttgtctac ttgccagctt tggtggcttg gtcagccttt agggtctttg
    33901 ggggtaatcc tattatcggt attgttttag gcttgatgtt ggtgtccaat gaattgccaa
    33961 acgcctgggt agttgcctct ggtggagatg ttaagccgct aaccttcttt ggatttgttc
    34021 ctgttgttgg ttatcaaggt accgttttac ctgccttctt tgttggtctg gtaggagcta
    34081 agttggagaa atggttgcac aaaaaggttc cagaagcttt ggacttattg gtcacaccgt
    34141 ttttaacgtt tgctattatg agtatcttgg gactatttgt gattggacca gtcttccatt
    34201 ctcttgaaaa ccttgttctg gctgggacac aggcagtctt acatttgccg tttggcattg
    34261 ctggtcttat tgttggtggt atccaacagt tgatcgtcgt tactggtatt caccatatct
    34321 ttaacttcct agaagcgcag ctgattgcca ataccggaaa agatcctttc aatgcttacc
    34381 taacagcagc aatagctgct caagctggag ctaccttagc tgttgctgtt aaaaccaaat
    34441 caccaaaatt aaaaggtttg gcttttccat cgaccttatc agctctttta gggattactg
    34501 aaccagctat ttttggggtc aatctccgtt atccaaaagt ctttgtttca ggtcttattg
    34561 gtggtgcctt aggtggttgg gtcgctggac tctttggcat tgcaggaact ggttttggga
    34621 ttacggtctt accaggaact ctcctttact tgaatggcca attattacaa taccttgtga
    34681 ctatgcttgt cggtcttggg gtagcctttg caattgctta tacttggggt tatcaagata
    34741 gagaaaccct tccgttacca gctgtcgaag ttgatcaaac ggctgatcag ccagccttag
    34801 cagaggaaac tttatatagt cctttgaatg gaacagttgt agatttagca gctgtttcag
    34861 accctgtttt ttcatcaggt gctatgggtc aaggcttagc aattaagcca gaagataata
    34921 ccctatactc accagttgat ggtaaagttg aaattgtctt tgaaacaggt catgcctatg
    34981 ctataacgtc aagtcaagga gcagaagtgt tacttcatat tggtattgat accgtgtcga
    35041 tggcaggaga tggctttgaa tctcttgtag cagtaggtca ggcggttaaa agaggtgatc
    35101 ttcttggaca ctttgatcct agcaagattg cagaagctgg actagatgac accaccatga
    35161 tgattgtgac caacagcgct gactatcaaa gcgtggacat tcttgctcaa ggacatattt
    35221 tgattggtga tcaagtggca cttatcaaat aaggacaaaa gagctaacta agcagctctt
    35281 ttgtcctgcc atgaatggca ggttggcaaa aaaatgagaa aagcctaaaa accttaaatt
    35341 tgtgttattt tacctatact ttgtaccttg ttttttttaa taaagtgttg ttatacttaa
    35401 ggcgaactat aggaatgcgc ttacatatat ggtatatcaa ctgggaagcc atgacttagt
    35461 accaaaaata aggagtgtcc aaatggataa acatttgttg gtaaaaagaa cactagggtg
    35521 tgtttgtgct gcaacgttga tgggagctgc cttagcgacc caccatgatt cactcaatac
    35581 tgtaaaagcg gaggagaaga ctgttcaggt tcagaaagaa ttatcttcta tagatagctt
    35641 gcattatctg tcagagaata gcaaaaaaga atttaaagaa gaactctcaa aagcggggca
    35701 agaatctcaa aaggtcaaag agatattagc aaaagctcag caggcagata aacaagctca
    35761 agaacttgcc aaaatgaaaa ttcctgagaa aatactgatg aaaccgttac atggtcctct
    35821 ctacggtggt tactttagaa cttggcatga caaaacatca gatccaacag aaaaagacaa
    35881 agttaactcg atgggagagc ttcctaaaga agtagatcta gcctttattt tccacgattg
    35941 gacaaaagat tatagccttt tttggaaaga attggccacc aaacatgtgc caaagttaaa
    36001 caagcaaggg acacgtgtca ttcgtaccat tccatggcgt ttcctagctg gaggtgataa
    36061 cagtggtatt gcagaagata ccagtaaata cccaaataca ccagagggaa ataaagcttt
    36121 agccaaagct attgttgatg aatatgttta taagtacaac cttgatggct tagatgtgga
    36181 tgttgaacat gatagtattc caaaagtaaa tggagaagct agtgacgaaa atctcaagcg
    36241 atctattgat gtttttgaag aaattgggaa attaattgga ccaaaaggtg ttgataaatc
    36301 gcggttattt attatggata gcacctatat ggctgataaa aacccattga ttgagcgagg
    36361 agctccttat attgatttgt tattggtaca agtctatggc gcaagaggag aacaaggtga
    36421 gtttcaaaac gataccaagt tagttaccga gactcctgaa gaacgatggc aaggttatag
    36481 caagtatatt cgtcctgaac aatacatgat tggtttttct ttctatgagg aaagagcagg
    36541 cagcggcaat ctttggtatg atattaattc tcgaaaggac gatgacaaag caaatggaat
    36601 taacactgac ataactggaa cgcgtgccga acggtatgca aggtggcaac ctaagacagg
    36661 tggggttaag ggaggtatct tctcctacgc tattgaccgt gatggtgtag ctcatcaacc
    36721 caaacaaata gctgaaaaag ataaacagaa tgtaaaaaat aatcagcctc agattccaga
    36781 aattactgat aacattttcc actcagatta tagtgtctcc aaggcattaa agacagttat
    36841 gctaaaagat aagtcgtatg atctgattga tgagaaagat ttcccagata aggctttgcg
    36901 agaagctgtg atggcgcagg ttggaactag aaaaggtgat ttggaacgtt tcaatggcac
    36961 attgcgattg gataatccag cgattcaaag tttagaaggt ctaaataaat ttaaaaaatt
    37021 agctcaatta gacttgattg gcttatcttg cattacaaag cttgaccaat ctgttttacc
    37081 cgctaatatg aagccaggca aagatacctt ggaaacagtt cttgaaacct ataaaaagga
    37141 taacaaagaa gaacctgcta ctatcccacc agtatctttg aaggtttctg gtttaactgg
    37201 tctgaaagaa ttagatttgt caggttttga ccgtgaaacc ttggctggtc ttgatgccgc
    37261 tactctaacg tctttagaaa aagttgatat ttctggcaac aaacttgatt tggctccagg
    37321 aacagaaaat cgacaaattt ttgatactat gctatcaatt atcagcaatc atgttggaag
    37381 caatgaacaa acagtgaaat ttgacaagca aaaaccaact ggacattacc cagataccta
    37441 tgggaaaact agtctgcgct taccagtggc aaatgaaaaa gttgatttgc aaagccagct
    37501 tttgtttggg actgtgacga atcaaggaac cctaatcaat agcgaagcag actataaggc
    37561 ttaccaaaat cataaaattg ctggacgtag ctttgttgat tcaaactatc attacaataa
    37621 ctttaaagtt tcttatgaga actataccgt taaagtaact gattccacat tgggaaccac
    37681 tactgacaaa acgctagcaa ctgataaaga agagacctat aaggttgact tctttagccc
    37741 agcagataag acaaaagctg ttcatactgc taaagtgatt gttggtgacg aaaaaaccat
    37801 gatggttaat ttggcagaag gcgcaacagt tattggagga agtgctgatc ctgtaaatgc
    37861 aagaaaggta tttgatgggc aactgggcag tgagactgat aatatctctt taggatggga
    37921 ttctaagcaa agtattatat ttaaattgaa agaagatgga ttaataaagc attggcgttt
    37981 cttcaatga
//
LOCUS       DAUELZ010000007        36384 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.007, whole genome shotgun sequence.
ACCESSION   DAUELZ010000007 DAUELZ010000000
VERSION     DAUELZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36384
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.007"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..246)
                     /locus_tag="VHI09_000241"
     CDS             complement(<1..246)
                     /locus_tag="VHI09_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120150.1"
                     /translation="MTYELCLEYGTYPLSRVDAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVAT"
     gene            448..1434
                     /locus_tag="VHI09_000242"
     CDS             448..1434
                     /locus_tag="VHI09_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEQ6120151.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPEFVGA"
     gene            1505..1696
                     /locus_tag="VHI09_000243"
     CDS             1505..1696
                     /locus_tag="VHI09_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120152.1"
                     /translation="MQRDTSERGHQAHFVMQSHQQRRRQHQLCLEPFQKNCEFLYYEV
                     EKVKLDSEDCLTINPLGMV"
     gene            1745..2164
                     /locus_tag="VHI09_000244"
     CDS             1745..2164
                     /locus_tag="VHI09_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEQ6120153.1"
                     /translation="MPFIAKQTLKSQLIPQDNLLAESRFNEIMDFLTGDFPLVFRPMF
                     NPHRYTISQDNQALEKVKQASYKRMDIAMTHLDGLIGESGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLAAFMAKMVEDSAVQQVLNAAH"
     gene            2401..3309
                     /locus_tag="VHI09_000245"
     CDS             2401..3309
                     /locus_tag="VHI09_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEQ6120154.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFSYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
     gene            complement(3427..3603)
                     /locus_tag="VHI09_000246"
     CDS             complement(3427..3603)
                     /locus_tag="VHI09_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEQ6120155.1"
                     /translation="MIKKYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            3737..4636
                     /gene="miaA"
                     /locus_tag="VHI09_000247"
     CDS             3737..4636
                     /gene="miaA"
                     /locus_tag="VHI09_000247"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEQ6120156.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DVGTAKATQEEQAAAVHHLIDIREVTESYSAYEFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRQELDQLSDQEVYELLQVKSITIKQLNRRRAIR
                     ALELSQFADDLENAETAYEPLIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVGFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            4709..5947
                     /gene="hflX"
                     /locus_tag="VHI09_000248"
     CDS             4709..5947
                     /gene="hflX"
                     /locus_tag="VHI09_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEQ6120157.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            5940..6575
                     /locus_tag="VHI09_000249"
     CDS             5940..6575
                     /locus_tag="VHI09_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEQ6120158.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            6590..7519
                     /locus_tag="VHI09_000250"
     CDS             6590..7519
                     /locus_tag="VHI09_000250"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEQ6120159.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFVVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            7519..8283
                     /locus_tag="VHI09_000251"
     CDS             7519..8283
                     /locus_tag="VHI09_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEQ6120160.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAQEIADM
                     FQVNTLASIHFACLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGKFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            8280..10490
                     /gene="recJ"
                     /locus_tag="VHI09_000252"
     CDS             8280..10490
                     /gene="recJ"
                     /locus_tag="VHI09_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEQ6120161.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYERGIRTE
                     EALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DERLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQVRTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKYPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            10641..11159
                     /locus_tag="VHI09_000253"
     CDS             10641..11159
                     /locus_tag="VHI09_000253"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEQ6120162.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            11240..11923
                     /locus_tag="VHI09_000254"
     CDS             11240..11923
                     /locus_tag="VHI09_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ6120163.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISDDFL
                     SAMNLWSDS"
     gene            11920..12576
                     /gene="nth"
                     /locus_tag="VHI09_000255"
     CDS             11920..12576
                     /gene="nth"
                     /locus_tag="VHI09_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEQ6120164.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            12648..13334
                     /locus_tag="VHI09_000256"
     CDS             12648..13334
                     /locus_tag="VHI09_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEQ6120165.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIA
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            13324..14112
                     /locus_tag="VHI09_000257"
     CDS             13324..14112
                     /locus_tag="VHI09_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEQ6120166.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMEGDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDFIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            14152..15258
                     /locus_tag="VHI09_000258"
     CDS             14152..15258
                     /locus_tag="VHI09_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEQ6120167.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLVSDLQTDGFATDFYQQNGIYLLKKQEEKLKD
                     LYQLALSRQEESPLIGHLEVKKREEIQEAFPGLVGFDNCLYASGAARVEGAALCETLL
                     KASGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLMPLGYQVDVRPQKGQ
                     LLDYDVHHIISDTYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKSCRVGIRAYTSDYSPFYGQVPGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSTGKA"
     gene            15316..16185
                     /gene="rfbA"
                     /locus_tag="VHI09_000259"
     CDS             15316..16185
                     /gene="rfbA"
                     /locus_tag="VHI09_000259"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEQ6120168.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHKLAQSLKKN
                     EYGQYLLRLIGEA"
     gene            16185..16778
                     /locus_tag="VHI09_000260"
     CDS             16185..16778
                     /locus_tag="VHI09_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEQ6120169.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            17022..18062
                     /gene="rfbB"
                     /locus_tag="VHI09_000261"
     CDS             17022..18062
                     /gene="rfbB"
                     /locus_tag="VHI09_000261"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEQ6120170.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(18145..19098)
                     /locus_tag="VHI09_000262"
                     /pseudo
     CDS             complement(18145..19098)
                     /locus_tag="VHI09_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            19227..19547
                     /locus_tag="VHI09_000263"
     CDS             19227..19547
                     /locus_tag="VHI09_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEQ6120171.1"
                     /translation="MTTESLIQNQIRVALSKAGHMVFRANVGKVKTVDGRLFDTGLPK
                     GFCDLFGFKPDGQIFFIEVKNETGRVRPEQKKFMEVMASRGALVGVARSVEDALKIVN
                     DTSR"
     gene            19531..19887
                     /locus_tag="VHI09_000264"
     CDS             19531..19887
                     /locus_tag="VHI09_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120172.1"
                     /translation="MTLVDDFYKQMEPSIKAFLDDNITIADKEEADRVYRSVKYYKKL
                     NRLPPPDVLEWFQRIYTTEEMIMLIKQSYRLKQKKTDEDDKIYEKWMFKNYGDVKLVK
                     KIKRINALAKAREMGL"
     gene            20144..20353
                     /locus_tag="VHI09_000265"
                     /pseudo
     CDS             20144..20353
                     /locus_tag="VHI09_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
     gene            20372..21262
                     /locus_tag="VHI09_000266"
     CDS             20372..21262
                     /locus_tag="VHI09_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEQ6120173.1"
                     /translation="MVTSRTYPEENLIKSTDLKYPITIDVTNKFQENISKLLEMLGVT
                     RKISVSVGMTLKTYAGYDVTLAEGNVPEGEVIPLSKVERKIHSEKKIELKKYRKATTG
                     EDIQMYGSNEAVTNTDNALVRQLQKKIRTDFVTALKTGTGTQDALGAGLQGALASAWG
                     KLQVLFEDYGSERAIVFANSLDVAEYIAKAGITTQTAFGLTYLVDFTGTVIISTNDVT
                     KGEIWATVPENIIFAYINPNNSELAKEFNLYGDPTGYIGMNHFQENTTLTIQTLLVSG
                     MLMYPERIDGIVKVTLTPGV"
     gene            21274..21567
                     /locus_tag="VHI09_000267"
     CDS             21274..21567
                     /locus_tag="VHI09_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEQ6120174.1"
                     /translation="MAYTVKARFFDLLDNSFLYEVGDSFPRKGYEPSKERLESLLSSN
                     NTEHKPFIEVSGNGNSLDGLKVDELKAKAAELGIELPSNAKKAEIIELLQAHI"
     gene            21581..21925
                     /locus_tag="VHI09_000268"
     CDS             21581..21925
                     /locus_tag="VHI09_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120175.1"
                     /translation="MDETKQAIIDRVRVRLADETSLKEELLEELTQTAIDRINLKVGD
                     VVFNPLFNSITVDVVVKMYRRMYFEGIDTEKADTISTKFIENVLAEYGEELASYKKDR
                     LAVLNKKVVRFL"
     gene            21922..22233
                     /locus_tag="VHI09_000269"
     CDS             21922..22233
                     /locus_tag="VHI09_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120176.1"
                     /translation="MRFVNFDLVTPQKTGKKDKLGNDITKDVVKRDAKGRFTEWSADD
                     VSLYGRDLTSSARKLLTNQVSKAEAKQASHVVIDGSKYKVESVKDLGRWRLLVIKGYR
                     L"
     gene            22230..22625
                     /locus_tag="VHI09_000270"
     CDS             22230..22625
                     /locus_tag="VHI09_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120177.1"
                     /translation="MRMSMTGMPALEVKLRSMSDKRWDRVVNKNLTEMFNRSARPPGT
                     PIGKNTKRHKSGELLRSRRLKKVNSSKGVITGNFGYIKDYAPHVEYGHRLVRNGKQIG
                     YVNGTKYLFNNVKKQREIYRQDMLNELRK"
     gene            22627..23037
                     /locus_tag="VHI09_000271"
     CDS             22627..23037
                     /locus_tag="VHI09_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5072 family protein"
                     /protein_id="HEQ6120178.1"
                     /translation="MLKKLGLVDLHASIKQKIEDKTGLMAYDHVPEDMPSPFYFIEVV
                     DKRPEDTKVMWCEVFTVWIHAIAEAGKSKIAIYDMIEKLEEALTEELVLPEEIDILRQ
                     SEVGMQSLQEDETGEMHAIVAYEIKVSYGFKVKI"
     gene            23049..23306
                     /locus_tag="VHI09_000272"
     CDS             23049..23306
                     /locus_tag="VHI09_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEQ6120179.1"
                     /translation="MVAFDQNVYCNFDASATKAIVCIKVINGKTKKGMFGGLASVSDY
                     SLEAPYDDGMTYSISLEGNGALIDLSTLSSEDAGKVTAMPS"
     gene            23319..23609
                     /locus_tag="VHI09_000273"
     CDS             23319..23609
                     /locus_tag="VHI09_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120180.1"
                     /translation="MDKELIIIDEKQYELKYNEKTIETVEALTGKAFMDVIVNNKGML
                     SLSMLRQYFSNALYAVEGGRVSSEQGSNVFTKVLNTKGYVYLASGRWYNSSC"
     gene            23959..25143
                     /locus_tag="VHI09_000274"
     CDS             23959..25143
                     /locus_tag="VHI09_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120181.1"
                     /translation="MQRLLDEAGKLSGMEFDINNFSDIIQAIHIIQDNLGVTGTTARE
                     ASETISGSFSQAKASVIDFMGSLGGDGSDVDAKMQAMIQSITVFADNVKEALGRIWDN
                     LPLQEYQKNLLLIAVVGGPILVVVGTIFGAIANIMTVVSGILTVLGMMTPIGWIIVAA
                     IAAIVAAVVFWDDIVAEVGQKFELVKIIGMAMWESFKDGVSSFGKLIQNVVGKIGGWF
                     AEAWNTASDTATRIFTRVGETISGIFSGVVSGIKSLINGAIGGINKVIGITNKLPGVN
                     IPKIPYLARGTSDWQGGLARINEGGRGELVHMPNGTTVVPHDVSMKYARESAKANSGI
                     AYVDSNDEIAKSALKLANDAIKRPVVLNINGREVARTTGGDMRDYLNSRDMTLKRLRG
                     EV"
     gene            25144..26628
                     /locus_tag="VHI09_000275"
     CDS             25144..26628
                     /locus_tag="VHI09_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEQ6120182.1"
                     /translation="MTTMTFNDVDLSSLITIMSVNRDIGNERQAETNDAPFVGLHVKK
                     IKTSAKIITVDFYLKDRTNEYALNQLKHKLAGIFNVNEQVKVTFSDEPDKYYLAIPIN
                     KISASDPIAWLSLVSLELLVPDGVAHSVSYKKITNYRQDGKKLLIDITNNGNVDAHPI
                     ITVKHNAENGYLAFVNKSSVFEVGDREIADAEIREKSVVAYDFKDERIVNALTAGKKN
                     VAILNDSTQVLDKNLLLNTVWGRKHLELEGSVAPSGNHAGTLTFDIPDGGSLYDYLWW
                     RQIFWVGTVSQLGFIKIMVSDENGHFLYGVETIKRTLGTKTEYNFLVTNGKGGYKITD
                     LRWTFDATHFETQNPFNEPRGWSDMTRADDTVSVFWWGSQNKRVFPELKGKKSKQVHI
                     AIGTIQGSPLVTHMYIDGFYYRKDKVPYEFNIPNAFGAGTEVVINAENDTVLVDNIPK
                     ANIIADGFFSFPKIPPGTSTLEVYSSSWSTANPDITLNFEERWL"
     gene            26629..30078
                     /locus_tag="VHI09_000276"
     CDS             26629..30078
                     /locus_tag="VHI09_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ6120183.1"
                     /translation="MLITIHNANLEKVAYIDNDKQDTLNYYDDKFSQYLNTANSTFEF
                     TVYKRGIKSDTIKEKAYLTLTERSFVSIKYNGKSYLFNVMTTDETDIEIRCYCENLNL
                     ELLNEYAGPYRAATQMSFVDYCNLFGILKNGAITIGTNETTDQKRTIEWTGQDTNLKR
                     LLSIANNFDAEIEFVTHLKNDSSLKSFVMNVYKKNDATNQGVGRRRDDIILQYGKNIE
                     SVRRKIDKTGIYNAIRPSGKITTTTTTTTAKQGSVQTGSVLWSGGNLTYAGHVMQSSV
                     VNTILSLCSKYKLLPSGVFSQLYLESFWGDTPVGRADNNWGGITWTGATTRPSGINVS
                     QGQPRAEGGHYNHYASVDDYLKDYTYLLAEQGIYAVKGKLTIDEYTRGLFRVGGATYD
                     YAAAGYDHYAPLMRDIRAGINRNNNGAMDNVDNQFKNGGSTSQNTTQIAAKTKAVLAE
                     ATGLKGQRVGSGQCYALAAWYAMKLDGPGLNGGVTSFRGLIGAGAAAAQIGTDYNWGQ
                     FGWKVVQPNKVADLITGSIVNIRANAGSPVFTGAWGHTVVVKSLSGDTLTVLEQNYNN
                     VQTVQEHTYSASAYLSVVQTVCYPPEIVKGRRVEGTAQAEQPQPETTTTSEEKEVLIN
                     PSLYREWKNESGQVEFYVKNSMLYAPLSKSLYPSAFTGIETDDNWIRKDLDVDTESEE
                     KLISVALADLRKHCYPAVTYEVSGFIGDLDIGDTIKINDPEYTPSLILEARVSEQHIS
                     FTEPNQNKTVFDNYRALESKVSQGLIDRMNELAEAAKPYDLRLMTDNGNVFLNGEGRT
                     ILTAELWKGNKKFDASYQFKRDGQLVGAGLQLAVDAKDVPADKPLIITVEAYLNNELI
                     ASKQITFTNSLGEQGPAGRGIVSTEDYYLASPNRTGVTSATSGWTKTPQEITETNKYH
                     WYYHVDVYSDGTRKETTPAIIGVYGDKGSDGKQGEKGNDGPMGPQGPAGALDEKQLQD
                     INNKIDGKADQNLTIEQINKLAELQSIANAELQAKASIDALASLQKQVQSAIAAMNAS
                     QKLSEQDLITASQRAIKATNDILDLKEQWNFIDNYMSASEEGLIIGSKDGTSSVRVAK
                     DRIAFYSAGAEVASITGGMLKIDNGMFVATLQVGHFREEMYKVDGVDKHINVTRYYET
                     IVG"
     gene            30083..31945
                     /locus_tag="VHI09_000277"
     CDS             30083..31945
                     /locus_tag="VHI09_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120184.1"
                     /translation="MAEFWSNNDRGYRIKLTIDQVSQNTLANSSQVRVKLDLLNTTTT
                     FTQYSCSAYVDLNGQRIDWSDSPSVLRYNSTISLIDRTITVNHNADGTKSFGFIASFS
                     GSGGWSPGTLTVGSGTFTLTTIPRSSSVVVSSGTIGSAITININRQSSSFKHNLRYQW
                     GNKTGTIASDVDTSAVWTIPLDFASDIPNSTSGSGTIYVDTYNDSTLTGTQQVPFTVT
                     VPDSMKPTLSSISLSDAHTVAGNVVSSADYFIQVYSDIRVNFESASGSYGSTIKGYYA
                     EIVGKGQSTEQNGGTLGNMLYDGQITIRAKVIDSRGRESQPVDKTVTVLKYFPPALSF
                     DVARSGYGSDTLTVTRRASIAPLSVFGTQKNTMTLSFSVAELGSSYFSANNGSASGAW
                     ANVSSLVNSSANLYGAFSPTKSYTVKGILSDKFSRTEFTFDVGTESVVMSIAKNGIGF
                     QKIWEKGAIDAKGDAYISGKLFVNNTEVKPSFDKTEILNMVYPVGAIYMSTSSANPST
                     FIGGTWQRYAQGRTIVGVSENETEFNYVGKTGGAKTHTLTNEEMPSHSHGDKTISSGG
                     RPISSNAGWDNTNVGLYKSTDYNQINAFNKSSGGDQPHNNLQPYITTYIWLRTA"
     gene            31956..32303
                     /locus_tag="VHI09_000278"
     CDS             31956..32303
                     /locus_tag="VHI09_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEQ6120185.1"
                     /translation="MLVITSNYPEQMPDGSVSGAKVMLDGIDDHAGWHIPLTLPKEYL
                     DKPQSEVMTLCEKMIYQQLKPQKALNEKFAKLDATIEKGENMLKFATGILNQIIAAKE
                     EETTDDETVEKMD"
     gene            32453..32776
                     /locus_tag="VHI09_000279"
     CDS             32453..32776
                     /locus_tag="VHI09_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120186.1"
                     /translation="MTIDVTLFAQWCVALTAIIVFLKWVSAPVKHVLDNNKKAMEALE
                     SAIVKISDDLKDNNFKWTESKNHRDRLQKVQDQHEIRIGIVEDRLISHDEQLKTLWKV
                     KEEKK"
     gene            32776..33108
                     /locus_tag="VHI09_000280"
     CDS             32776..33108
                     /locus_tag="VHI09_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEQ6120187.1"
                     /translation="MEELIQVITGSALSILTIFAGIVVKLIKDYLLKKGGEKAVKIAE
                     IVARNAVEAVEQIAYDKDIKGIEKLTEAKIAVRDELSKHNVYLSDKQMEVFIEAAVKR
                     MNDSWKGQ"
     gene            33110..33874
                     /locus_tag="VHI09_000281"
     CDS             33110..33874
                     /locus_tag="VHI09_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ6120188.1"
                     /translation="MATVSEVISYWRGLADTNQGYDADLAWGWQCADVTNGTTTNFFG
                     VTLWGNAIDLLDSAKAQGLEVIYDAPGINPKAGDLFVMYTYAHPYGHTGIVIADSDGY
                     TIQTIEQNVDGYSDNNGDGINDQFQVGGPARYVTRAFSDGDGYIVGWIRPPYSDTSSE
                     KQSQSQAPSGFRKLKDEVGTFEVMVPALNVRREPSLNGEIVACYQYGMTGTYDSVYVG
                     DGYIWVSYVGASGMRNYMAVGDADGDYNVNPYCKFY"
     gene            33886..34488
                     /locus_tag="VHI09_000282"
     CDS             33886..34488
                     /locus_tag="VHI09_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120189.1"
                     /translation="MGYNSTNLKQVDGGDVIKQGDTSSLFSFNLLDENNNVIDLNGKQ
                     ATIYFTRNRKTYLTKTTDVIDNKVDFTINKILEIGTYYIEVHCDGYVFPSDDSVTLDV
                     RRSGQKYVVSTDLITDTTIQKLSADIEYLKSKVTQNQHLFEQVSPQTEWTITHNLIKY
                     PSVTIVDSAGNEVFGSVEYISTTKIIVRFSAPFAGKAILN"
     gene            34499..35272
                     /locus_tag="VHI09_000283"
     CDS             34499..35272
                     /locus_tag="VHI09_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120190.1"
                     /translation="MSTFLTNINLNKNELQNAVVQNLGSEPQDPKTGQFYYDSVANKL
                     KIYNGTSWVALDKETAESIVNLINSGESKIDKSKVDGLEEAINRASMTGQAIATALNE
                     DTGGVTVNQDKITGLTDKLAEIATNKASATRESQDALQEAKVYADQKVAGLVDSASED
                     YNTFKELETFIKQNAKGLAAISNVAKKFSKTFGDARAKEFTITHNLKTRDVNVTIRET
                     NFPYAIVYADIKVTDENSILIATGAEVPTQNQYTVTITG"
     gene            35282..35503
                     /locus_tag="VHI09_000284"
     CDS             35282..35503
                     /locus_tag="VHI09_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120191.1"
                     /translation="MRYIGESQLSKDLVTKDYVDNKVTNLASKDDIVNLGKIKDSQTG
                     AFLEVKIVPNGQVPYDTTGMIVFERSGGK"
     gene            35503..36162
                     /locus_tag="VHI09_000285"
     CDS             35503..36162
                     /locus_tag="VHI09_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120192.1"
                     /translation="MSVQEIPDEIFYRLIEQTVDTKLVWDKDTQIGSYYISIHDKYSF
                     ESGRDVNIYKILSSTQADVYKANKYNYQSSSKPDTSVPQPKPPEQPPLKNKSLYQSIT
                     IDGLPIARVICNNHVIWSQSVEQQEVYNITAYVDASVSKLNDSAIIDYNRDFAAYGVN
                     WSRVDRVAVEVDDKIVAKLQAPVYDFGVRGGTTMSVEKWDYPSNTIEKYKNPTFKISQ
                     H"
BASE COUNT        11522 a         6524 c         7957 g        10381 t
ORIGIN      
        1 agtagctact tcttgatata gtatccgaat ctgcgctctt ttttcaggca tgttaaaacc
       61 aacataatgg aattgtgatt caatagttac aaataaatca tggaacaggt gattcatagt
      121 ttctaattta tggcaaagca gtcgatcttc ttgaatgaat gttgggggat tttgatcttc
      181 tccccagtaa gcatcaacac gtgacaacgg ataagtccca tattctaaac aaagttcata
      241 agtcattttg gcctccttgt tttatcttag cattttctaa ataaagcttc taatgatttg
      301 cttaaaggag taatggattg ccatctcatc tcatcaaaaa taaattgaga tctattttgt
      361 gaaaaaaaaa gacaaacaaa tggcttgcct agacttgttt ttcctagata taataggaag
      421 taactgaata acaatgaagg agaaaaaatg tcagaaaaaa tcagagtttt actctattat
      481 aaatatgttt caattgaaaa tgcgcaagag tacgctgcga aacatctaga attttgtaaa
      541 tctattgggc ttaaaggtcg tattttgatt gcggatgagg ggattaatgg aactgtttct
      601 ggagattatg aaacaactca gaagtatatg gattgggtcc acagcgatga gcgttttgct
      661 gatctttggt tcaagattga cgaggaaaat caacaagctt tccgtaaaat gtttgttcgt
      721 tataagaaag agatcgtgca ccttgggtta gaagacaata actttgatag tgatattaat
      781 cctttggaaa caacaggtga gtatttaaat cctaaacaat ttaaagaagc cttgcttgat
      841 gaagatactg ttgttttaga tacgcgtaat gattatgagt atgacttagg tcacttccgt
      901 ggagcaattc gtcctgatat tcgcaatttc cgtgagttac cacaatgggt acgtgacaac
      961 aaagataagt ttatggaaaa acgtgttgtt gtgtactgta cgggtggtgt tcgctgtgag
     1021 aaattctcgg gttggatggt tcgagaaggc tttaaagatg ttggacaatt acacggtggt
     1081 attgcaacat atggaaaaga tccggaagtt caaggtgaac tttgggatgg tgcgatgtat
     1141 gtttttgatg atcgtatctc ggtgccaatt aatcatgtta atccaactgt cattagtaaa
     1201 gattattttg atggaacacc ttgtgaacgt tatgtcaatt gtgcaaatcc gttttgtaac
     1261 aaacaaattt ttgcttcaga agaaaacgaa acgaagtatg ttcgagggtg ctcgcctgaa
     1321 tgtcgtgcac atgaacgtaa tcgctatgtg caagaaaatg gtttatcacg tcaagaatgg
     1381 gcagaacgtt tagaagctat tggagaatcc ttgccagaat ttgtaggtgc ctaatatctc
     1441 aaaacaaatc aggaaactgg tttgtttttt gtgatacaga tgatgagacc aaattgcaaa
     1501 gactttgcaa cgagatacca gtgaacgtgg acaccaagct cattttgtca tgcagagcca
     1561 tcaacagcga agacgtcaac accaactttg tctagaacct tttcaaaaga attgtgagtt
     1621 tctgtattac gaggtagaga aggtaaaact ggattctgaa gactgcctta ccatcaatcc
     1681 tttgggaatg gtttaagctt tacagcttga tgatggtcaa attgtcacac aggcaggtat
     1741 tatcttgccc tttatagcga aacaaaccct gaaatctcag ctcattcccc aagataatct
     1801 tttggcagaa agtcgattca acgaaatcat ggacttctta acaggtgatt ttccccttgt
     1861 ttttaggcca atgttcaacc ctcatcgcta cactatttct caagacaatc aagcgcttga
     1921 aaaagtcaaa caagcttctt acaagcggat ggatattgct atgacgcatt tagatggtct
     1981 tattggggag agtgggcatg tttatcgaga tcaacaaacc attgcagatg cttatgctta
     2041 cgccatggcg ctatggtctc aaaaaacacc aaaatcttac gagaattatc cgcatctggc
     2101 agcctttatg gcaaaaatgg tggaagattc agctgttcaa caagtgctta atgcagctca
     2161 ctaaatcaaa aagaagaagt aaagatcaga ccaagtcttc tctactcttt catttttcta
     2221 gagaagagtg gtcactcctt agctttttaa gcagtttcct agttttgcga gaatgtaaaa
     2281 aaacaatggc aagagcctta aattttcttt cgcgggtggt gtcaaaaatg ctcgaaatgt
     2341 gataaaataa acaagattat gtcactaaaa atagctgtta ataggcagaa aaggacactt
     2401 atgaaacacc ttaaaaaccc accattggtc atgtcaggat tggcattagg aacgttgtca
     2461 tttggcaatt tgttggctac ttatgtttcg atttttagtt atttgggaat tctggcagct
     2521 ctttttattt atggtatctt gcttgttggc atggttagga accttaacga taccaaaatg
     2581 cagttacgac aacctctaat tgcctctgtt tttccaactt tttttatgac agggatgttg
     2641 ttgtcttcac tctttctcaa agtgacagga gggtgttggt taggctttct cacttggtgg
     2701 ctattctttt tgggaaatct tgtcctgatt gcttattacc agtatcgttt tgtcttttct
     2761 ttttcatggg ataacgtttt tccttcctgg tccgtgcttt ttgttggaat tgcaatggcg
     2821 gcattaacag cacctgctag taggcagttt ttattaggac aggtgatttt ttgggtttgt
     2881 cttctcttaa ctgctgtgat tttacctttt atggccaaaa agacttatgg gattggtcta
     2941 ggtcaagcag taatgcccaa tatttcaacg ttttgtgccc ctctgtcctt actttcagcg
     3001 tcttatttag caacctttcc tagaccacaa gtaggaatgg ttatctttct tctcatcagc
     3061 tcacaactgt tatatgcttt tgtggttgta cagcttcctc gcttactcaa tcgtcccttt
     3121 aacccaggtt tttcagcttt tacctttccg tttgtgatta gtgccacttc cttaaaaatg
     3181 accttatctt ttttaggttg gcaaggttta ggttggcagg ttcttttgct aggagaggta
     3241 ttgttggcga cagctttagt cacatatgtt tatggggctt acctgagatt tctatttcaa
     3301 aataagtaaa gcagctaata tcttggctca tcaagagaca gttactacta actagttatc
     3361 gagattacta aaaaagaggc cggcacttta atgtgctagt ctcttttaaa ttgatccatt
     3421 gtcacgttag cgagctacac gacgacgcgc agcttttttg cggttttctt caatgaaagc
     3481 agctttttct tcttcagggt caatgattgt tttttttaca gcaaagacag caccagcaac
     3541 agtagcagca gttgctagga cgccagtagc aagaccttta acaaattgat atttcttaat
     3601 catgatcatt ctccatttct agtgaaggac ctcagtggtc cttaatctta tgttataata
     3661 tatggtagcg aaaaataagc taattttcaa gtaaaaggcg tttttggaca cgcttttcta
     3721 atagaaagac aagaaaatga caaaaataaa aatagttgtg attgttggtc cgacagcggt
     3781 tggaaaaaca gcacttggga ttagtttagc caaggctttt aatggagaaa ttatttcagg
     3841 agatagccag caagtttatc gacagttgga tgttggaaca gccaaggcca ctcaagaaga
     3901 gcaagcagca gcggttcatc acttaattga tattcgtgag gtgacggagt cttattcggc
     3961 ctacgagttt gtccaagatg ctcaaaaagc tatttcagac attgttagtc gtgggaaatt
     4021 accaattatt gtaggtggaa ctggtcttta cttacaaagt ttactcgaag gttatcatct
     4081 aggaggtcaa gttgaccaag aggcggtaaa agcttatcga caggagttag accagctgtc
     4141 tgatcaagag gtgtacgagc ttttgcaagt aaaaagcatt actattaagc agcttaaccg
     4201 caggcgggcc attcgtgcct tagagttaag tcaatttgca gacgacttag agaatgccga
     4261 gacggcttat gagccgctta ttatcggctt gaatgatgac cgccaagtca tctatgaccg
     4321 tatcaatcag cgcgtggatc gtatgcttga aaacggcttg ttggaagaag caaaatggtt
     4381 atatgagcat tacccgactg ttcaggctag tcgtggcata ggttataagg aattattccc
     4441 ctattttgta ggtgaaatga ctttggcaga agctagtgat cagttaaaac aaaacacgag
     4501 acgttttgcc aagcgacagc taacttggtt ccgcaatcga atggctgttg gcttcactgc
     4561 cataacggct cctgattacc cacaggtagt acatgatagg gtcagggatt ttcttggtca
     4621 gaaagagaag tcatgacgca aaattagcag aatctgttga agatatgtta taatagaatt
     4681 tacggaattt ggaggattgg taaaggtgat gattgagaca aagcgtcagc aagaaagagt
     4741 gattctttta ggagttgagc ttcagacaac agaacatttt gatatgtcca tgacagaatt
     4801 agctaatctt gccaaaacag ctggcgtcaa agttatggca agtttcagcc aaaaacgaga
     4861 acgttacgac agcaaaacct ttatcggctc agggaaactg gatgagatta aggcgattgt
     4921 ggaagctgat gagattgatg cagttattgt gaacaatcgg ttgactgcta ggcaaaatgc
     4981 taatctagaa gctgttttag aggtaaaagt cattgaccgt atgcagttaa tcttagatat
     5041 ttttgccatg cgtgccagaa gccatgaggg caaattacaa gtacaccttg cccaattgaa
     5101 atatatgtta cctcgtttgg ttggacaggg catcatgtta agtcgtcaag caggtggtat
     5161 tgggagccgt ggaccaggtg aaagccaact agagttaaat cgccgttcga ttcgtcatca
     5221 gattgctgat attgaacgcc aattaactca ggtagagaaa aatcgtcaaa caattagaga
     5281 tcgtcgtgtg ggttcagata cctttaaaat tggcttgatt ggctacacca atgctggcaa
     5341 gtcaaccatt atgaacttgt tgacagatga cagccactat gaagccaatg aattatttgc
     5401 taccttagat gcaactacaa agcaactcta tttagaaaat caatttcaag caaccttaac
     5461 agacaccgta ggctttattc aagacttgcc aacggaatta gtggctgctt ttaaatcaac
     5521 cctagaagaa agcaaatatg tggatttgct gttacatgtg attgatgcga gtgatcccaa
     5581 tcatagtgag caagaaaaag tggttttaaa ccttctaaaa gagctagaca tgcttaatat
     5641 tccgcgttta gctatttata acaaggtgga tatagcagaa caattcacag ctactgcttt
     5701 tccaaatatc aggatttcag cccgttcaaa ggatagcaaa attctcttga gacgtttaat
     5761 cattgaccaa attcgagacc agtttgttcc ttttagaatc aaagtgcatc aagacaaggc
     5821 gtacaagcta tatgatttga atcgagttgc tcttttagac cactacacgt ttgaccaaga
     5881 aatagaggat atttcgggct acatttcccc aaaacagcaa tggagacttg acgattttta
     5941 tgaataatta tattgattta gcaaaaacct atggtggatt tactagtctg gataccaact
     6001 acctaaacca tctgttagcc agtttgacag accagcaaaa actagctttt atcacaccac
     6061 caccaagtgt tattaatgcc tactttgctg aaatctacca gaaacagtca ccgcaagctg
     6121 cgactgatta ttattttaat ctttctaagg ctttggggct ttttactgac caaccatctt
     6181 ttgaggaaga aaagcccttt gtacgcctca atttgtctgg aaaagcttat ggttttgctt
     6241 accaaaatga ccaagaagtt gcattggtat tttctgaaaa agcagagcct aagaaacccg
     6301 aactcttctt tgaattaaca caaattttcc cacaatatat ggtttatgaa gacaaaggcc
     6361 agcttaaaat gcaggctaag cagtttgagc aaggagaatg tgaagacatt acgcctgatg
     6421 acacactctt gtcgaagatt taccgtctgg caaacggtat caccatgctt aaggggttta
     6481 atgtagagga gttatgggct cttagccaaa cattttctgg tcaaaagtat tatgattttg
     6541 cccaacgaga atttatgatt tacatcaccc aatagaaaga atagattgaa tggaattaca
     6601 atttttagga actggagctg gtcaaccagc caagcagcgc aatgtgtcaa gtcttgcctt
     6661 gaaattacta gacgaaatca atgaagtctg gatgtttgat tgcggtgaag gaacgcaaag
     6721 acaaatttta gaaacgacga ttaagccacg aaaaataaga aagattttca ttactcattt
     6781 gcatggggac cacatttttg gattaccagg atttttatca agtcggtctt ttcaagctag
     6841 tgaagaacag acagatcttg atatttatgg accaattggc attaaaactt acgtgttaac
     6901 tagtctaaaa gtttctggag cgcgtgtacc ttatcagatc cattttcatg aatttgatga
     6961 caaatcatta ggtaaaataa tggaaacaga taagtttgtg gtctatgcag agcgtctggc
     7021 gcataccatt ttttgcatgg gttatcgagt agttcaaaaa gacttagaag gaaccctaga
     7081 cgcagaagcc ttaaaagcag cgggtgttcc cttcggacca ctctttggca aaattaaaaa
     7141 tggtcaagac gttgagttgg aagacggccg cctgatttgt gccaaagatt atatttctgc
     7201 ccctaaaaaa ggcaaaatca tcaccattat cggcgatacg cgcaaaacgt ctgccagtgt
     7261 taaactcgct aaagatgcag atgttttggt tcatgaatcc acctatggta agggtgacga
     7321 gcggattgcg agaaatcacg ggcattctac caacatgcag gcagcacaaa tcgctcatga
     7381 agcaggcgca aaacgcctct tgctcaatca tgtatcagct cgcttcttag ggcgtgattg
     7441 tcgccagatg gaaaaagatg ccgccactat atttgaaaat gttaaaatgg tacaagattt
     7501 agaggaagtg attatttaat ggcacaaaga atcattgtta tcacgggagc ttctggagga
     7561 ctggctcagg caattgttaa gcagttaccc aaggaagaca gcttgatttt attaggacgt
     7621 aacaaagaac gcctagaaca ctgttatcag catattgaca acaaagaatg ccttgagttg
     7681 gatattacca atccagtagc cattgagaaa atggtcgccc agatttacca gcgctatggc
     7741 cgtattgatg tcttgattaa taatgctggc tatggagctt tcaaaggctt tgaagagttt
     7801 tctgcccaag aaatagctga tatgtttcag gttaacaccc tagcgagcat tcactttgct
     7861 tgcttgattg gtcagaaaat ggcagagcag gggcaaggcc atctcattaa tattgtgtcc
     7921 atggcaggct tgattgcgtc agccaaatcg agcatttatt cagccaccaa gtttgccctt
     7981 atcggatttt ccaatgccct tcgcttagaa ttagcggata aaggggttta cgtgaccacc
     8041 gtgaatccag gtcccattgc caccaagttt tttgaccaag ctgacccgtc tggacattat
     8101 ttggaaagcg ttggtaaatt tactctccaa ccaaatcaag tggctaagcg tttggtttct
     8161 attatcggga aaaataaacg agaattgaat ttgcccttta gtttagcggt gacccatcaa
     8221 ttttacaccc ttttccctaa attatctgat tatcttgcaa gaaaggtatt taattataaa
     8281 tgattaaatc caaatatagc tggaaaataa aagacaagaa gccagatgat ggcttcttta
     8341 agctagcaaa gacaaaaggt ctaacccaga ccgccgctca gttgatttat gagcgaggga
     8401 taaggactga agaagcctta gatgagtttt taacagcgga cttgtctcaa ctgcatgatc
     8461 cttatttgct acatgacatg gcaaaagctg tgccaagaat tcgtcaagct attgaagagg
     8521 gcgaacgaat tctcatttac ggcgactatg atgctgatgg catgacgtcg gcttctattg
     8581 tcaaagaaac cctagacatg atgggagcag agcctttggt ttatcttccc aaccgcttta
     8641 cagatggcta tggccccaac cagagcgtct ataaatattt tattgagcag gaagctgtgt
     8701 ccctaattat cacggttgat aatggcgtgg ccggacatga agcgattaga tatgctcaag
     8761 aacaggaagt tgatgtcatt gtaactgacc atcatagcct gcctgaagaa ttgccagaag
     8821 cctttgccat cattcatcct gagcatcctg atgctgatta tccctttaaa cacttagctg
     8881 gctgtggcgt tgcctttaaa ttggcaacag ctttgctaga aagtttacca acagactgtc
     8941 tagacttggt agctattgga accattgccg atatggtaag cttgacaggt gaaaaccgtg
     9001 ttttagtcaa aaatggtctg gccatgctca aacacaccga aagagtgggc ttgcaggagt
     9061 tgatgtcctt gtctcctatt gatttggaac attttaatga agacgccatt ggctttcaga
     9121 ttgctccgca attaaatgct ttagggcgct tagatgaccc taatccagcc attgagctac
     9181 tgacgggctt tgatgaccaa gaagctcaag cgattgcctt gatgattaag aaaaaaaatg
     9241 aagagcgtaa ggccttagtc caagacattt ttgatcaagc gatggctatg gttgatcctc
     9301 aaaaacctgt tcaagtcttg gcacaagctg gctggcatcc tggtgttctt gggattgtcg
     9361 ctggtcgtat catggaaact attggacaaa cggtcgtcgt tttgaccatt gataatggct
     9421 ttgcaaaagg ctcggccaga agtttagaag ctattaatat ttttgaagcc ttgaatggca
     9481 agcgtgagtt atttacagct tttgggggac atgctggtgc agcaggaatg accctgcccg
     9541 ttgacaactt ggaagctctc tcagactttc tttgtcagtt tgtgatagaa cgtggcttag
     9601 atcagactgc caaaaatacg ttaactattg atgaaagact gtctttggac gaccttagcc
     9661 ttgatatttt aaagagttta gataaattag ccccttatgg gatggatcat caaaagccgg
     9721 tcttttatgt caaggacata agggttagtc aagtgcgtac catagggcaa gaccagagcc
     9781 atcttaaatt taaagtcagt cagggcaaag cttcctttga cgtactggct tttggtcaag
     9841 gaagtcaact gcaagagttc cgacaagcta ctggtttaga attagcagtt accttatctg
     9901 tcaaccattg gaacgggaac acgagtctcc aattcatgtt agtagatgct cgtgttgacg
     9961 gagtgcaact tcttgactta aggacaaaaa cagccaaagt tcctgaaggt attccgacta
    10021 ttgaagaaga tccaaatgcc agagtcattc tgattaatga catccctgaa gatttcaaaa
    10081 cctggcgcaa ccagtttgtt cacaaggatt ttgacgccat ctattttaaa aatcagatga
    10141 aatatcctta ttatttaaca ggatttggca gcagagagca gtttgccaag ctctacaaaa
    10201 ccatctatca gtttcctgaa tttgatttac ggcataaatt gacagagttg agtcactatt
    10261 taaacataga aaaactttta ctgattaagt tgattcaaat ttttgaagag ttgtcttttg
    10321 tgacgattga cgatgggctg atgacggtta acccccaagc gcaaaaacga gagatttcag
    10381 agagccacat ttaccaagac ttaaaagaat tggtgaaatt tcaagaaatc atggctttgg
    10441 ctagcccgaa agagatgtat gattatttgg tgaaatcaaa cgatcaataa gtaaagcagt
    10501 tgacagtagt tattgaggta gttacgaatg aagtatcaga taaacaataa tgtcagaagt
    10561 cttattttac gaaaaatatg tttcgtgata taatgaagag tattttatat ttggcaattt
    10621 gccactagaa agaattaact atggatttaa caaattacat tgcatcgatc aaagattacc
    10681 ctaaagctgg tattacgttt cgagatatct cacctttaat ggctgacggt aaagcatata
    10741 gctatgctat tcgtgaaata gcacagtacg cttgtgataa agatattgac atggttgttg
    10801 gacctgaagc gcgtggtttt atcattggtt gtcctgttgc agttgagtta ggaattggtt
    10861 ttgctcccgt tcgtaaacca ggaaaactgc caagagatgt tgtctctgct gattacgaaa
    10921 aagaatatgg tcttgatacc ttaactatgc atgcagatgc tattaagcct ggacaacgtg
    10981 ttttgattgt cgatgattta ctagcgactg gcggtacggt aaaagcaact attgaaatga
    11041 ttgaaaaact tggtggtatt gtagctggtt gtgccttcct tattgaatta gaaggtttaa
    11101 atggtcgcca tgctattagg aactatgact ataaagtctt aatgcagttt cctggataag
    11161 cacattaatc agcatctgat taggggattt aaagtagtaa aggctataga ttcgatttta
    11221 gccttttgat tgagggatta tgagtttttt agaacattat aagtcaggta atttggtcat
    11281 cccaagtgcc ttgctttttc attacaaaga tctttttaag agctcagatg attttttggt
    11341 ctggcagttt ttctatcttc aaaatacgac aaagcgagac gacttagcgc ccagtcaaat
    11401 tgctcatgcg cttggaaaat cagttgctga tattaataaa atcatttcat ctttgactag
    11461 tcaagggctt ttagatatgc ggacaattga acttactgga gaaattgaaa ttatttttga
    11521 tgcgagtcct gtgttagcca agttagatca attgtttgtc agtcaaacgg caactgaaat
    11581 tgacaaacaa gaaacaccaa accatttcaa acgattagtt gatgaatttg aacgtgaatt
    11641 aggacgattc ttgagtcctt ttgagttaga agatcttgaa aaaacgcttc gtgatgataa
    11701 aacagatcct gatttgatac gagaagcttt aaaagaagct gtcttcaacg gcaaaacaaa
    11761 ttggaaatat atccaggcta tcttacgtaa ttggcgaaaa gaaggtattg ttaatcttcg
    11821 acaagtagaa gaacgtagac gtgttagaga aggcgaagat ctttctcaag tgacgatatc
    11881 agatgatttt ctatctgcga tgaatttatg gagtgattca tgagaattgg aaaagcaaga
    11941 ttagcaaaag tcctgaccat cattggtcaa atgtttcctg aggctaaggg agagttagac
    12001 tgggaaacac cttttcaatt gttgattgcg gtgattttat cagctcaaac gacagataag
    12061 gccgtcaata aggtaactcc tggactatgg cagtcttatc cagaaataga agacttagct
    12121 tttgctgaac tttctgatgt tgaaaatgct ctgagaacga ttggactcta taaaaataag
    12181 gccaaaaata tcattaaaac tgctcaggct attcgtgatg attttaaggg tcaagtgcca
    12241 aaaacccaca aagaacttga aagtttaccg ggtgttggcc gaaaaacagc caatgtggta
    12301 ttggcagagg tttatggtgt tccagctatt gcggttgata ctcatgtggc tagagtctca
    12361 aagaggctca atatttcatc accagatgcc gatgtcaagc agattgaagc agatttgatg
    12421 gctaaaattc caaagaaaga ttggattatt actcaccatc gattgatttt ctttggacgc
    12481 taccattgtt tagccaaaaa accaaaatgt gagatttgtc ccgttcagtc ttactgcaag
    12541 tactatcaaa atacttatgg aaaatcgaaa gcctagcctt tggttttctt tttttatgga
    12601 ggtaattttg ctataataga atcattacgt tttagaaaga aatcaagatg gatagtcaat
    12661 tatcaaaccg tttggcccaa gttgcggcct atgttccaaa aggtgtcaaa ttattagatg
    12721 ttggcagtga tcatgcctat cttcccatat ttttggtaga aactaatcag atttcagcag
    12781 ccattgctgg cgaagtggtt aggggtccct acgaatctgc tcttaaaaat gtcacacagt
    12841 ctggccttgc cgagcatatt caggtgcgct tagccaatgg ccttgcagcc tttgaagaag
    12901 ctgatgatgt gacagccatt accatttgtg gcatgggtgg ccgtttgatt gctgatattt
    12961 tagaggcagg taaggaaaaa ttacagggca ttgaacgtct ggttttacaa cccaacaatc
    13021 gtgaggatga tttacgggct tggttatcgg ttaacgcttt taaaattgtt gcagaaacca
    13081 tcatggctga aaatgacaaa tattatgaaa tcatcgttgc tgagcatggt gaaaaggcct
    13141 tatccgcgac agaattgcgt tttggtccat acctttctca ggaaaaatca gtcgttttta
    13201 aagagaagtg gcaacgagaa atggataagt tagcttatgc cttgtcctgt atcccagaag
    13261 aaaaaactca agagcgtcag cttctcttga caaaaataca acaaataaaa gaggtgattg
    13321 ttcatgaaag ctaaaaccct aattgatgcc tatgaagcct tttgcccact tgatttatct
    13381 atggaaggag acgttaaagg tcttcaaatg ggttcattgg ataaagacat tcgtaaggtt
    13441 atgattactt tagatatccg agagtcaaca gtagcagaag ccatcaaaaa tgaagttgat
    13501 ttcattataa ctaagcatgc tcccatcttt aaaccgctta aagatctggt ttcatcccct
    13561 cagcgtgata tccttctaga tttggtcaag catgacattt ctgtctatgt gagccatacc
    13621 aatattgaca ttgtaccagg tgggctaaat gattggtttt gtgacctgct agaaatcaag
    13681 gaagcaactt acttgtctga aaccaaagaa ggctttggca ttggtcgtat tggaacagtt
    13741 aaagaacaag cgttagaaga gttagcaagt aaggtcaaaa gggtgtttga tttagatacc
    13801 gtgcgactta tccgttacga taaggaaaac cctttgattt caaagattgc tatctgtggc
    13861 ggaagtggtg gtgaatttta tcaggatgct gtgcaaaaag gcgcagacgt ttatattaca
    13921 ggggatatct attaccatac agcacaggag atgctgacag aaggactatt tgcggttgac
    13981 ccggggcatc atattgaggt gctttttacg gagaaattaa aggagaaact ccaagggtgg
    14041 aaagaagaga acggttggga tgtcagcatt atttcaagca aagcctcgac caatccattt
    14101 agccatttat aggctgttaa ggtcttaaaa taagataaaa ggataccagg aatgaaaata
    14161 gccattattg gggcggggat tgtaggatca accgccgctt attatttaca acaaagtggt
    14221 caaaaagaag ttaccatttt tgaccatggt caaggacaag ccactaaggc tgctgctggt
    14281 attattagtc cttggttttc aaagcgtcgc aataaagttt ggtatcgcat ggctcgttta
    14341 ggagccgatt tttatcaaca gttagtcagc gacttacaga ctgatggctt tgcaacggat
    14401 ttttaccagc aaaatgggat ttacttatta aaaaagcaag aggagaagtt aaaagatctt
    14461 tatcaattag ctttaagtag acaagaagaa tcccctttaa tcggtcattt ggaagtaaaa
    14521 aaacgtgagg agatacaaga ggcatttcca ggcttagttg gctttgataa ctgtctgtac
    14581 gcttcaggag ctgctagagt ggagggagcc gctttatgtg agacgttact caaggctagt
    14641 ggttatccag ttattcgcca aaaagtcact ctgaaacagc aaggaagtgg ttatgagatt
    14701 gctggtcatt attttgatca ggtcattctg gcagcaggag catggctacc tgacttattg
    14761 atgcctttag gctatcaggt tgatgtgcga ccacaaaagg gacaactctt ggattatgac
    14821 gttcatcaca tcatcagcga cacttatccc gttgttatgc cagaaggtga aatagacctc
    14881 attcctttca atcaaggtaa gatttctgtt ggtgcgagcc atgaaaatga taaaggttat
    14941 gatttggagc ctgattggca ggtcttagaa aaacttgaaa tgcaagctct cacttatttg
    15001 ccgttattaa aagaagctac tcaaaaatca tgtcgtgtag ggataagagc ttataccagt
    15061 gattattctc ctttttatgg tcaggttcca ggattgaaga acctttatac agctagtggt
    15121 ttagggtcat cgggattaac agttggacct ttgattggct atgaattagc tcaattattg
    15181 ctaggacacg aaggactttt gacgcctagc gactattccc ctgagcctta ccttttgcgg
    15241 tctactggta aagcgtgaca ctgctttcaa aaaatgatag aatgataggg tatatagaag
    15301 aataggagca ctactatgaa aggtattatt cttgcaggag gttcagggac acgcttgtat
    15361 ccactgaccc gtgccgcatc taaacagttg atgcccattt atgacaaacc aatgatttat
    15421 tacccattgt caaccttgat gttggcaggg attaaagata ttttaatcat ttcaactccc
    15481 caagatcttc ctcgtttcga agaattgcta ggagacgggt cagaatttgg cattagctta
    15541 tcctataagg aacaaccaag cccagatggt ttagcacaag ctttcattat tggagaggag
    15601 tttatcggtg atgaccgtgt tgctcttatt ttaggagata atatttacca cggtaatggc
    15661 ctcactaaaa tgcttcaaaa ggcagctgcc aaagaaaaag gagccaccgt ttttggttat
    15721 caagtgaaag accctgagcg ctttggagtg gtggaatttg atgagaacat gaatgctatc
    15781 tctatcgaag aaaaaccaga agtgcctaag tcccactttg cagtgactgg actttatttc
    15841 tatgacaatg acgtggtaga gattgctaaa aacattaaac caagcgcgcg tggtgagttg
    15901 gaaattacag acgtcaacaa ggcttacttg gaacgtggtg acctctccgt tgaattgatg
    15961 gggcgtggtt ttgcatggct agacactgga acgcatgaaa gtctacttga agcggctcaa
    16021 tatattgaaa cagttcagcg cttgcaaaac gctcaagtgg caaatctgga agaaattgcc
    16081 tatcgcatgg gttatatcag taaagaagat gtccataaat tggcgcaatc tttaaagaaa
    16141 aacgaatacg ggcaatactt gcttcgtttg attggagaag cttaatgaca gaaacttttt
    16201 ttgacaaacc attggcttgc cgagaaatca aagaaatccc aggcttacta gagtttgata
    16261 ttcctgttcg tggggataat cgtggctggt ttaaagaaaa tttccaaaaa gaaaaaatgt
    16321 tgccaattgg ctttccagaa cgtttttttg aggaaggaaa actacaaaac aacgtttctt
    16381 tttcacgtca gcatgtgctg cgtggacttc atgctgaacc ttgggataaa tacatctcag
    16441 ttgctgatga tggcaaggtt ttaggagctt gggttgatct tcgtgaggga gagacttttg
    16501 gaaacgtcta ccagacagtg attgatgctt caaaaggaat gtttgttcct agaggggttg
    16561 ctaatggctt tcaagttctt tcagagactg tctcttacag ttatcttgtc aatgactact
    16621 gggctcttga cttgaaacct aagtatgctt ttgtaaacta tgctgaccca agtcttggga
    16681 ttacttggga aaatctagca gctgcagaag tttctgaagc agataaaaac catcctcttc
    16741 ttagtgatgt caaaccactg aaaccaaaag acctataggg aaacaacata ctcagatgat
    16801 tgggattaat catttgaggt ggatatgagg ggagttattc ttctctacct caggtaacaa
    16861 gtaacaacta aaaatgatga aaggaaagtt cagtttctct ctttgagctg aacatgggac
    16921 tcaaatctta gaaagcaatg ttttcttaga cctaaaacgt ctttgttaga gtcattactt
    16981 ttaatagatt ttttcggaaa cgaaaggtcc ctctatatct tatgtataaa aatattatcg
    17041 taactggtgg agctggtttc atcggttcta actttgtaca ctatgtctac aataaccacc
    17101 cagatgttca tgtaactgtc cttgataaat tgacatatgc aggtaaccgt gctaacattg
    17161 aagctattct tggtgatcgt gttgagttag ttgttggtga tatcgctgac gctgaattgg
    17221 tagataaatt ggctgccaaa gcggatgcta ttgttcacta tgcggctgag agccacaacg
    17281 ataactcatt ggaagatcca agtccattta tccatacaaa ctttatcgga acttacactt
    17341 tgcttgaagc agctcgtaaa tacgatatcc gtttccacca cgtgtcaact gatgaagttt
    17401 atggagatct tccacttcgt gaagaccttc caggacaggg cgaaggacca ggtgaaaaat
    17461 tcactgctga aacaaaatac aatccatcat caccttactc atcaactaag gcagcttctg
    17521 accttatcgt taaggcatgg gtacgttcct tcggtgtgaa agcgaccatt tcgaactgtt
    17581 caaataacta tggaccatac caacacatcg aaaaattcat tccacgtcaa attacaaata
    17641 tcttggcagg aatcaaacca aaactttatg gtgaaggtaa aaacgtccgt gactggattc
    17701 atactaatga tcattctaca ggagtatggg ctattttgac taagggtcgt atcggtgaaa
    17761 cataccttat tggtgccgac ggcgagaaaa acaacaagga agttcttgaa ttgattcttg
    17821 aaaaaatggg tcaaccaaaa gacgcttatg atcacgtaac tgaccgtgct ggtcacgatc
    17881 ttcgttacgc tattgattct acaaaattgc gtgaagaact cggctgggaa ccacaattta
    17941 caaacttttc agaaggtttg gaagaaacta ttaagtggta cacagaaaat gagacatggt
    18001 ggaaagcaga aaaagatgct gtagaagcca agtatgctaa aactcaagaa gtgattaaat
    18061 aaaacattaa agagccttgt catatcaacg tttgttgaag atgtcaaggt tttttcttgt
    18121 gttttggggc atttttgggg catattatag ccgatttaag atgtcgacga cttcattttt
    18181 catgcttttc gtgacgtggg tatagatttg ttgagtagtt ttcgagtcag cgtgtccaac
    18241 tctatccatg atagttttta agggcacttt gttttctgca aggcgactca ccagggtatg
    18301 acggaagatg tggctggtga gttctttttg aatcggtttt tctagtctct tatttgccgc
    18361 tctgatagag gtgttgaggg cgttgtcttg aataggaacg ccatttctgg aaatgaagat
    18421 gtagcccatg tccttgtaat taggattagt atttttttca agagcattaa ttttcagtat
    18481 atcctgaata atttcttttt cacgtttggt tatcaatgtt tcacgccaac tggaatttgt
    18541 tttaggggtg gttttgatag cgtttctata gccatttgag gtgtagtcaa gagttccgtg
    18601 gatttcaatg atatcatttt tgatattgtc tggctgtata gctagagctt ctccaatgcg
    18661 gcagccgttt aagctcataa attctgccag ataagccatt ttaatcgatc ctttacgtct
    18721 atacatttca tctaatagcc ttttgagttc atctttttct aaaaacttct gggcaacgtc
    18781 ttcaaagtct ttgatggtct taacaggttt tgcgagttgg gtactaataa ctggattgat
    18841 tgagacatac tctaaagcga tagcgtagtc gaaggtttgt ttgagcagct gccgtgctcg
    18901 tttacgtttg tcataactac ctggtatttt gtcgataata ctttgcaggt atttcgtggt
    18961 taccctggca atcatcactt ctggatcaat taagtttcgc aattctctga ttctaaaatc
    19021 aagagagcgg acagaagttg atttgagtga tttctgatga tgttcccacc attcgttcat
    19081 gacatctagt aggtaggcat atatataaag agagttgaaa aatgcggtaa ctcggtaacc
    19141 aaatcaccaa atagcttggg agagtaagga ggagaaggac accgcaaccg gtaaccaagt
    19201 gagtaaccgg tgtccgaaat aattatatga caacagaatc actaatccaa aaccaaatcc
    19261 gtgttgctct atcaaaagcg ggccatatgg tttttagagc taacgttggt aaagtcaaaa
    19321 cagtagacgg tagattattt gatacgggac tacctaaagg tttttgtgac ttgtttggat
    19381 ttaagccaga cgggcaaata ttcttcattg aagtaaaaaa cgaaacaggt cgagtaaggc
    19441 ctgaacagaa aaaatttatg gaggtaatgg catctagggg agctctggta ggagtagcta
    19501 gatctgtgga agatgcctta aaaatagtca atgacactag tagatgattt ttacaaacaa
    19561 atggagccgt caatcaaagc gtttttagac gataacatta ccatcgcaga caaagaagaa
    19621 gctgacagag tctatagatc tgtcaaatac tataaaaaac taaacagatt gccgccacct
    19681 gatgtattgg agtggttcca acgaatctac acgacagagg aaatgataat gttaatcaag
    19741 cagtcttacc gccttaaaca aaaaaagaca gatgaggatg acaagattta cgaaaagtgg
    19801 atgtttaaaa actacggtga cgttaagctc gttaaaaaaa tcaaacgcat aaacgcatta
    19861 gctaaggctc gggagatggg tctatgaaaa gacacagaca gtggcataac gatattaaat
    19921 atacacctag atcttacgat aatctgttgc cttacgatat atcagagctg ttaatagctc
    19981 acagatgcaa aataaagatg tctgatgacg ttttagcgga caggataggt atttatactt
    20041 ggcaattaaa agcgctctta gaacgcagaa tattgccaaa tgagagcgtg tgtaaaacga
    20101 tagtaaatta tttgagggag gtggagagat gctgacggaa gatactgatg cagataaaac
    20161 caaaaatgca gtagaagcgt tttctgaggc attttccgaa gcaatcgaaa aagaagtcaa
    20221 agaacgcctt aaatcgccaa cacctaaaaa atcaaatggc aatactggac tgacaaaaga
    20281 acaaatttta gcagtcaaag atactgcgga acgacaaaaa ctcattgccg aaaatatcgg
    20341 cctattccaa taaaacagga gaaattaaca catggtaaca tcacgtactt acccagaaga
    20401 aaatctaatc aaatcaacag accttaaata ccctatcact attgatgtca caaacaaatt
    20461 ccaagaaaat atctcgaaat tacttgaaat gctcggggtt actcgtaaaa tctcggtatc
    20521 tgtgggtatg acccttaaga catacgctgg ttatgatgtc acattagcag aaggtaacgt
    20581 cccagaaggc gaagttatcc cgttatcaaa agtagagcgc aaaatacact ctgagaaaaa
    20641 aatcgaactt aaaaaatatc gtaaagcaac aaccggcgaa gacatccaaa tgtatggctc
    20701 taacgaagct gtaacaaata ctgacaacgc acttgttcgt cagttgcaaa agaaaatccg
    20761 cacagacttt gtgactgctc ttaaaacagg tacaggaaca caagatgctc ttggtgcagg
    20821 attgcaaggg gctttagctt ctgcttgggg taaactgcaa gtgctctttg aagattatgg
    20881 ttcagagcgt gcgattgtgt ttgctaatag ccttgatgtc gctgaataca tcgctaaagc
    20941 tggcatcact actcaaaccg cattcggatt gacttacctt gtcgatttta cagggacggt
    21001 cattatctcg acaaacgatg tcacaaaagg ggaaatctgg gcgacagtac cagaaaatat
    21061 catctttgct tacatcaatc caaataattc tgaattagct aaagaattta atctttacgg
    21121 tgatccaacc ggctacattg gaatgaatca cttccaagaa aatacaacgc ttactatcca
    21181 aacgctgctt gtatctggta tgttgatgta tccagaacgc attgacggaa ttgttaaagt
    21241 aactcttaca ccaggcgtct aagggggtaa tctatggctt acacagtaaa agcacgtttt
    21301 tttgacttgc ttgacaatag ctttttgtac gaggttggcg acagctttcc tcgcaaaggc
    21361 tatgagccaa gcaaagagcg cttagaaagc ttgttatcta gcaacaatac agaacataaa
    21421 ccatttattg aggtgtcagg taatggcaat agcttagatg gtttaaaagt ggatgagtta
    21481 aaagctaaag cggcagagct aggaatcgaa ctcccaagta atgctaaaaa agctgaaatc
    21541 atcgaattac tgcaagcaca tatctagaaa gtaggttgac atggacgaaa caaaacaagc
    21601 aattattgat cgtgtccgtg tccgtttagc agacgaaacg tctttaaaag aggagttatt
    21661 ggaggagtta acgcaaacgg ctatcgaccg tattaattta aaagtcggcg atgtcgtttt
    21721 taaccctctt tttaattcca ttactgttga tgttgttgtc aaaatgtatc gtcgtatgta
    21781 ttttgaagga attgacacgg aaaaagcaga cacaatatca actaaattta ttgaaaacgt
    21841 tttggcagaa tatggcgagg agttagcgtc gtataaaaag gaccgtctag ccgtcttaaa
    21901 taaaaaggtg gtgcggtttt tatgagattt gttaattttg acttagtaac cccacaaaaa
    21961 acagggaaaa aagataagct tggcaatgac atcactaaag atgtcgtcaa gcgtgacgct
    22021 aaaggtcgtt ttactgaatg gtcggctgat gacgtgtcct tatacggtcg agatttaacg
    22081 tctagtgcac gcaaattgct gactaatcaa gttagcaagg cggaagccaa acaagcgtca
    22141 cacgttgtaa tagatggctc aaaatacaaa gttgaatccg ttaaagatct tggtagatgg
    22201 agactactcg tcattaaagg gtatcgctta tgaggatgtc aatgacaggt atgccagcct
    22261 tagaggtaaa actaaggtca atgagcgata agcgttggga tagggttgtc aacaaaaacc
    22321 tcacagagat gtttaacaga tcagcaagac cgcctggtac accgattggt aaaaacacta
    22381 aaaggcataa gtctggcgag ttgttgcgct ctagacgtct caaaaaggta aactcatcaa
    22441 aaggtgttat cacaggtaat tttggatata tcaaggacta tgcacctcat gttgaatacg
    22501 gtcatagact tgtccgtaac ggtaaacaga ttggctatgt taacgggaca aaatacctgt
    22561 ttaataacgt caaaaaacag cgtgaaattt ataggcaaga catgctaaac gaattaagga
    22621 aatgacatgt taaaaaaact gggactggtt gatttacatg cctcaattaa acaaaaaatt
    22681 gaagataaga cagggttgat ggcttatgat cacgtaccag aagacatgcc atcgcctttt
    22741 tattttatcg aggttgtgga taagcgacca gaagacacaa aggtcatgtg gtgcgaagtg
    22801 tttacggttt ggatacatgc catagcggaa gctggaaaaa gcaaaatcgc tatttatgac
    22861 atgattgaaa aactcgaaga agcgctcaca gaagaattag ttttgccaga agaaatcgac
    22921 attttgagac aatcagaagt cggcatgcag tccttgcaag aggacgaaac tggcgaaatg
    22981 catgcaattg tagcctacga aatcaaggtt tcgtacggtt ttaaagtaaa aatttaaaag
    23041 gagaaataat agtggcattt gatcaaaatg tttattgtaa ttttgacgcg tcagcaacaa
    23101 aagctattgt ttgtattaaa gttattaacg gtaaaaccaa aaaaggcatg tttggagggc
    23161 ttgcgtcggt aagtgattat agtttagaag ctccttacga tgatggtatg acatactcaa
    23221 tcagtctcga aggcaatgga gcattgattg atttatcaac attatcatca gaagatgctg
    23281 gaaaagtaac ggctatgcct agttaatgag aggaatgaat ggataaagaa ttaattatta
    23341 tcgatgaaaa acagtacgaa ctaaagtaca acgaaaaaac cattgaaacc gtcgaagctt
    23401 taactggcaa agcgtttatg gatgttattg taaataacaa agggatgttg tcgttatcta
    23461 tgttgcggca atatttttcc aatgctcttt atgcggtaga aggtggtcgt gtgtctagcg
    23521 agcaaggctc aaacgttttt actaaagtgt taaacacaaa aggttatgtt taccttgcca
    23581 gcggtcgctg gtataacagc agttgttaaa gagtatgcaa accttgaaca gtcaatcggt
    23641 ggtgttgaga ctttgtttaa aaagtctgct aaagctgtgc aaaaaaatgc aaatacagcg
    23701 tttagacgag ctggtgtatc tgctaatgag tacatggagc aagtaactag cttttctgct
    23761 agtctaattt catctcttgg cggagataca gcaaaggctg tcggtgttgc tgatactgcc
    23821 atggtcgaca tgtctgacaa cgctaataaa tttggtacaa acatcgggtt aatccaaaat
    23881 gcctatcaag gctttgctaa aggcaattac acgatgttag ataaccttag attgggtttt
    23941 ggcgggacga aagaggagat gcaaagattg ctggacgaag ctggtaagct aagcggcatg
    24001 gaatttgaca tcaataactt ttcggacatt atacaagcaa tccacattat ccaagataat
    24061 ttaggcgtaa caggaacgac agctagagaa gcgtcagaaa ctattagcgg ctcattttcg
    24121 caagcaaaag cgtcagtaat cgatttcatg ggctctcttg gcggagatgg cagcgatgta
    24181 gatgcaaaaa tgcaagcaat gatacagtcg attactgtgt ttgcagataa tgtcaaagag
    24241 gctttagggc gcatttggga caatttgcct ttacaagagt atcaaaaaaa cctactgctt
    24301 atagctgtcg ttggcggacc tatccttgtt gtggtcggga ctatttttgg agctattgct
    24361 aatatcatga ctgttgttag cggtatttta accgttctag gcatgatgac gccaataggt
    24421 tggatcatag tggcagccat agcggctatt gtcgcagctg tcgttttttg ggacgatatt
    24481 gtcgcagagg tcggccaaaa atttgagtta gtcaaaataa tcggcatggc gatgtgggaa
    24541 agttttaaag atggtgtatc tagctttggc aagttaatcc aaaatgtcgt tggcaaaatt
    24601 ggcggatggt ttgcagaagc gtggaatact gcatcagaca cagctactag gatttttact
    24661 agggttggag agacgatcag tggtatcttt agcggtgttg tttctggcat taaaagtttg
    24721 ataaatgggg caatcggtgg catcaataag gttattggca ttactaacaa actgcccggt
    24781 gtcaacatcc caaaaatccc ataccttgca cgaggtacat ctgactggca aggtggtctt
    24841 gctcgtatta atgagggcgg acgaggggaa ttagtacaca tgccaaacgg tacgacagta
    24901 gtgccgcatg acgtaagcat gaaatacgct cgagaatctg ctaaagccaa ttctggcatt
    24961 gcttatgttg atagcaatga tgagattgcc aaaagcgcct tgaaattagc taacgatgct
    25021 attaaacgcc ctgttgtgtt aaatatcaac ggccgtgagg tcgctagaac aacaggtgga
    25081 gacatgagag actacctaaa tagtcgagat atgacattaa aacgattgag aggtgaggtt
    25141 tagatgacta caatgacttt taatgatgtg gacttatcta gcctcatcac tatcatgtct
    25201 gtcaatcgag acatcggtaa cgagagacaa gccgaaacta acgacgctcc atttgttggt
    25261 ttgcatgtaa aaaagataaa gactagcgca aaaataatta cagttgattt ttatttaaag
    25321 gacaggacaa acgagtacgc attaaaccag ctaaaacaca aattagctgg tatttttaat
    25381 gtcaacgagc aagtcaaggt gacttttagc gatgagcctg acaaatatta tctagctata
    25441 ccgataaata aaatatcggc tagcgaccca atagcttggc taagtcttgt gagcttggag
    25501 ctacttgtcc ctgatggcgt ggcccattct gtgtcctata aaaaaatcac gaattacaga
    25561 caagatggta agaagctgct gattgacatt acaaacaacg gtaatgttga tgcgcatccg
    25621 atcattactg ttaagcacaa cgccgagaat ggttatttag cttttgttaa taaatcatcc
    25681 gttttcgagg tcggagacag ggaaatagca gacgctgaaa tccgtgaaaa gtcggtcgtc
    25741 gcttatgatt ttaaagacga aagaatagtc aatgccttaa cagcaggtaa aaagaatgtt
    25801 gctatcttaa atgactcgac acaagttctt gataaaaatt tattactaaa taccgtctgg
    25861 ggaagaaaac atctcgagtt agaaggaagt gttgcgccaa gtggcaacca tgctggaacg
    25921 ttaacgtttg atattcctga cggaggtagc ttatatgact atctttggtg gagacaaatt
    25981 ttttgggttg ggactgtttc ccagcttggc tttatcaaaa tcatggtatc agacgaaaac
    26041 ggacattttt tatatggagt agaaactata aaaagaacgt taggaacaaa aacagagtat
    26101 aactttttgg tcactaatgg aaaaggtgga tataaaataa ctgatttacg ttggactttt
    26161 gatgctactc acttcgaaac acaaaacccc tttaacgaac ccagaggttg gtctgatatg
    26221 acaagggctg atgacactgt atctgttttt tggtggggtt ctcaaaacaa gcgagtattc
    26281 ccagaattaa aaggcaaaaa atctaaacag gtacacatcg caatcggcac tattcaaggc
    26341 agtcctttag taactcacat gtatattgat ggattttatt atcgcaagga caaagtaccg
    26401 tacgaattta atattccgaa tgctttcggg gcaggaacag aagtcgttat taatgccgaa
    26461 aatgacacag ttttagttga taacattcca aaagctaaca tcatcgccga tggctttttt
    26521 agttttccta aaattcctcc tggaacatca acattagagg tttattcctc tagttggtct
    26581 accgccaacc cagatattac gctcaatttt gaagaaaggt ggctataatt gcttataaca
    26641 attcacaacg caaatttaga aaaagttgct tatattgata acgataagca agacaccttg
    26701 aattattatg acgacaaatt ttcacagtat ttaaatacgg ccaattcgac atttgagttt
    26761 acggtttata agcgaggaat taagtccgac acaatcaaag agaaagccta tctgacactt
    26821 acagaaaggt cttttgtgtc gattaaatat aatggcaagt cttatctgtt taacgtaatg
    26881 acaacagacg agacagacat cgagatacgt tgttactgcg agaacctcaa tctcgagttg
    26941 ctcaatgagt atgcaggacc ttatagggcg gcaactcaga tgtcttttgt cgattattgt
    27001 aatttgtttg gcatccttaa aaacggtgct attaccattg gcactaatga gacaaccgac
    27061 caaaaacgca caatcgaatg gactggtcaa gataccaatc ttaaacgctt gttatctatc
    27121 gccaataatt ttgatgcgga aatagaattt gtaacgcatc ttaaaaacga ttctagtctt
    27181 aaatcgtttg tcatgaatgt ctacaagaag aacgacgcta ctaatcaagg tgttggtcgt
    27241 agacgagatg atattatttt gcaatatggc aaaaatatcg aaagtgtcag acgtaagatt
    27301 gataaaacag gcatttacaa tgctataaga ccaagcggta aaataacaac gactacaacc
    27361 acaacgactg ctaaacaagg ctctgtgcaa acagggtctg ttttgtggtc tggcggtaac
    27421 ttgacttatg caggccatgt gatgcaatca tctgttgtta acactatttt aagcctatgt
    27481 agcaaataca agctgttacc gtccggtgtt tttagccagc tgtaccttga atcattttgg
    27541 ggagataccc cagtcggaag agccgataat aactggggtg gcatcacttg gactggcgca
    27601 acaactagac caagcggtat caatgtctca caagggcaac ctcgagccga gggtggtcat
    27661 tataaccatt acgcaagcgt tgatgactac ttaaaagact atacttacct cttggcagag
    27721 caaggtattt atgccgtaaa aggtaagcta accattgatg agtacacaag aggtctgttt
    27781 agggtcggtg gcgcaacata tgattatgct gcagctggat atgatcatta tgcacctttg
    27841 atgcgagaca tcagagcagg tattaaccgt aataataacg gcgctatgga taacgtcgat
    27901 aaccaattta aaaatggcgg ctcgactagt caaaatacta ctcagatagc tgctaaaaca
    27961 aaagcggtac ttgcggaagc aaccggactg aaaggtcaac gagtaggctc tggtcagtgc
    28021 tatgcgttag cagcttggta cgcaatgaaa ttagatgggc caggtctaaa tggtggtgta
    28081 actagtttta gaggacttat tggtgctggt gcagcagctg ctcagattgg tacggactac
    28141 aactggggtc agtttggctg gaaagttgta caaccgaata aagtcgcaga cttaatcaca
    28201 ggctcgattg ttaacatcag agcaaacgct ggcagtcctg tttttacagg cgcttggggg
    28261 catactgttg ttgttaaatc tctatctgga gatacgctca cagtcttaga gcaaaactat
    28321 aacaacgtgc aaacagtcca agagcataca tatagcgcta gcgcttattt atcagttgta
    28381 cagacagtct gttacccgcc agaaatcgtt aaagggagac gtgtcgaagg cactgcacaa
    28441 gcagaacagc cacaaccaga aacaaccacg acatctgaag aaaaagaggt tttaattaac
    28501 ccatcgcttt accgtgagtg gaaaaacgaa tcaggacaag tcgaatttta cgttaaaaac
    28561 agcatgctct atgcaccttt gtctaaatcg ctttatccat cagcgtttac aggaattgaa
    28621 actgacgata attggatacg aaaagactta gatgttgata cagaaagtga agaaaagctt
    28681 atctctgttg ctctcgcaga tctgagaaaa cattgttatc cagcggtgac ctatgaagta
    28741 tctggtttta ttggtgattt agatattggt gacactatca aaatcaatga tccagaatac
    28801 acgccaagcc taattttaga agcaagggtt agcgagcaac acatctcgtt tacagagcct
    28861 aatcaaaata agaccgtctt tgataattac agagctttag agagcaaagt ctcacaaggt
    28921 ttaattgacc gcatgaacga actagcagaa gctgctaaac cttacgactt gcggttaatg
    28981 acagataacg gaaatgtgtt tttaaacggc gaaggtcgta cgattttaac cgctgaactt
    29041 tggaaaggta acaaaaagtt tgatgcaagc tatcaattta aacgtgatgg tcaattagtc
    29101 ggcgctggat tgcagttggc agttgacgct aaggatgtac cagctgataa acctctaatc
    29161 attactgttg aggcttattt aaataatgag ctgattgcaa gtaaacagat tacgtttact
    29221 aactcgcttg gagaacaagg accagctgga cgtgggattg tctctacaga ggactattac
    29281 ttagcgtcac caaatcgtac aggtgtcaca tctgcaacat ctggttggac taagacgcct
    29341 caggaaatta ctgagactaa taaatatcat tggtattatc acgttgatgt ttattcagat
    29401 ggcactcgaa aagagactac accggctatt attggtgttt acggcgataa aggttcagat
    29461 ggcaagcaag gagaaaaagg gaacgatggt ccaatgggtc cgcaaggtcc tgccggagcg
    29521 ctagatgaaa agcaactaca agacatcaat aataaaattg atggcaaagc agaccagaat
    29581 ttgaccatcg agcaaatcaa taaattagcc gagttacaat ctattgccaa cgctgagtta
    29641 caagcaaagg ctagcatcga cgctttagct agcttacaaa agcaagtaca gtctgcaata
    29701 gcggcaatga atgctagcca aaagttatca gagcaagacc ttattacagc tagtcaacgt
    29761 gctatcaaag caaccaacga catcttagac ttaaaagagc aatggaattt tattgacaat
    29821 tatatgtcag cgtctgaaga gggacttatt atcggctcta aagacggtac aagttccgtg
    29881 cgtgttgcca aagaccgtat tgccttttac tcagctggcg cagaagtcgc ttcgattact
    29941 ggtggtatgc tcaaaattga taatggtatg tttgtggcta ctttgcaagt tggacatttt
    30001 cgcgaggaga tgtacaaagt tgatggggta gataaacaca taaatgttac aagatattac
    30061 gaaacgattg tggggtgatt aattggcaga gttttggtca aacaatgata gaggttaccg
    30121 catcaaactt acgattgatc aagtatccca aaatacatta gcaaacagta gccaagtgag
    30181 ggttaaactt gatttgctaa atacaacaac aacttttact caatatagtt gttctgcata
    30241 tgttgactta aacggtcagc ggatagactg gtcagactca ccatcagtac ttagatataa
    30301 ctcaacaatt tctctgatcg atagaacaat aacagttaat cataatgctg atggtactaa
    30361 atcatttgga tttatcgcta gctttagcgg ctctggtgga tggtctccgg gtactttgac
    30421 tgttggtagt ggtactttta cacttactac catcccacgc tcaagctccg ttgttgtttc
    30481 ttctggtact attggcagtg ccatcactat caacatcaat agacaaagct ctagttttaa
    30541 gcacaacttg agataccagt ggggtaataa aacaggtact attgcaagcg atgtggatac
    30601 ttccgccgtt tggactatcc ctcttgattt tgctagtgac atcccaaact caacaagtgg
    30661 tagcggcact atctatgtag atacctacaa tgactcaacg ctaactggta ctcaacaagt
    30721 gccatttacc gtaactgtac cagacagtat gaagccaaca ttatcaagta ttagcttgag
    30781 tgatgctcat accgttgcag gcaacgtagt atcaagtgcg gattatttta tccaagtata
    30841 ctctgacatt agagtcaatt ttgaatcggc aagtggctct tatggttcga cgattaaagg
    30901 ttattacgcc gagattgtcg gtaaaggtca gtctactgag caaaatggcg gaacactggg
    30961 gaatatgctg tatgacggac agattaccat cagagcaaag gttattgata gtcgaggtcg
    31021 tgaatcgcaa cccgtggata aaacagtcac ggtgctcaag tactttccgc cagctttgtc
    31081 gtttgatgtc gcaaggtctg gttatggttc tgacacgcta acagttacaa gacgagcgtc
    31141 tatcgctccg ttaagtgttt tcggaacaca aaaaaatacc atgacgctta gttttagtgt
    31201 tgcagaatta ggttctagtt atttttctgc taataacggc tctgcaagtg gggcatgggc
    31261 aaatgtttct agtttggtaa actcgtctgc caatctttat ggtgctttct cgccgacaaa
    31321 gtcttatacc gtcaaaggta tcttgtctga caaatttagt cgcacagagt ttacgtttga
    31381 cgtcggaaca gaatcagttg tgatgtcaat cgctaaaaat ggcatcggat tccaaaaaat
    31441 ttgggaaaag ggtgctattg acgctaaagg agatgcttat attagcggta agttatttgt
    31501 caataataca gaggttaaac cgtcgtttga caagacagag attttaaaca tggtttatcc
    31561 tgtcggagct atttacatga gcacatcatc agccaatcca tcaacattta tcggcggcac
    31621 ttggcaaaga tatgcacaag gtaggactat tgtcggggtc tccgaaaacg agacggaatt
    31681 taattacgtt ggtaaaactg gtggagccaa gactcataca ttaaccaacg aagagatgcc
    31741 aagtcacagt cacggggata agactattag ctcgggtggt agacctatct catcaaatgc
    31801 tggatgggac aatacaaatg ttggtttata taaatcaaca gattacaatc aaataaacgc
    31861 ttttaacaag tcttctggag gtgatcaacc acataacaac ttacaaccat acatcacaac
    31921 gtacatttgg ctgagaacag cttagaggag aatttatgtt agttatcaca tcaaattacc
    31981 cagaacaaat gcctgatggt tccgtatctg gtgcgaaggt gatgcttgac gggattgatg
    32041 atcacgctgg ttggcatatc ccgttgacat tacccaaaga atatttagac aaacctcaaa
    32101 gcgaggttat gacactttgc gaaaagatga tttatcaaca attaaaacca caaaaagcat
    32161 taaacgaaaa gtttgctaaa ctcgatgcaa ccattgaaaa gggtgaaaat atgcttaaat
    32221 ttgcgactgg aattttaaat caaattatcg cagcaaaaga agaggaaaca acagatgatg
    32281 aaactgttga aaaaatggat taaaaggaga tatatcatga tgattaatta tttcgcaatg
    32341 caaattgagt ttggttggat tactttggaa gatgtaccta aaaaatatcg tgacaaagta
    32401 aaacagcttg ttgaatcagg gaatatcgga acagaataac gagaggcctt atatgacgat
    32461 agatgttaca ctatttgcgc aatggtgtgt agcgcttact gcaataatag tatttttaaa
    32521 atgggtgtct gcacctgtca aacacgtttt agacaataat aaaaaggcta tggaagcctt
    32581 ggaaagtgct atcgtcaaaa tatctgacga cttaaaagac aacaatttta aatggacaga
    32641 atccaaaaac catcgtgaca gactacaaaa ggtccaagat cagcacgaaa tcagaatcgg
    32701 aatcgtagaa gatagactta ttagtcatga cgagcagcta aaaacgctct ggaaagtaaa
    32761 agaggaaaaa aaataatgga agaattaata caagttatca ctggctcagc tctatcaatt
    32821 ttgacaatct ttgcaggtat tgtagtcaag ttaatcaaag attatttgct taaaaaaggc
    32881 ggagaaaaag cggtcaaaat cgccgaaatt gtggctcgca atgccgtgga agcagttgag
    32941 cagattgctt atgacaaaga catcaaaggt attgaaaaac tgacagaagc taaaattgcg
    33001 gttcgtgacg aactgtctaa acacaatgtc tatttgtcag acaaacaaat ggaagtcttt
    33061 attgaagcgg ctgttaaacg catgaacgac agttggaaag gtcaataata tggcaacagt
    33121 tagcgaagta attagctatt ggcgtggatt agctgataca aatcaaggtt atgatgccga
    33181 ccttgcgtgg ggctggcaat gtgctgatgt gactaatggg acaactacta acttttttgg
    33241 agtgacgctt tgggggaatg cgattgactt gctagacagt gcaaaggcgc aagggttaga
    33301 ggttatctat gacgcccctg gaataaatcc caaagctggt gatttgtttg ttatgtacac
    33361 ttatgctcat ccttatggtc atacaggaat tgtgattgct gatagtgatg gttatactat
    33421 ccaaacaatt gagcaaaatg tagatggata ctcagataat aatggagatg gcattaatga
    33481 ccagttccaa gttggcggac cagcacgata tgtcactcgt gccttttcag acggtgacgg
    33541 ttatattgtc ggttggatta gaccgcctta tagcgatacc tcatctgaaa agcaatcaca
    33601 atcacaagcg ccatcaggat tccggaaatt aaaagacgag gtcggaacat ttgaggttat
    33661 ggtcccagcg ctaaacgttc gccgtgaacc aagcttaaat ggcgagattg tagcttgtta
    33721 ccaatacggc atgacaggaa cttatgattc tgtctatgtt ggcgatgggt atatttgggt
    33781 gtcttatgtc ggtgcatctg gcatgagaaa ttatatggct gttggagacg ctgatggcga
    33841 ttacaatgtc aatccatatt gtaaatttta ttaaaagggg gttatatggg atataactca
    33901 accaacctta aacaggttga cggtggcgat gttatcaaac aaggtgatac atcgtcactt
    33961 tttagtttta atttgttgga cgaaaacaac aacgttattg atttaaatgg caaacaagcg
    34021 acgatttact ttacacgtaa tcgcaaaacc tatctaacca aaacaactga tgttattgac
    34081 aacaaagttg attttacgat taataagatt ttggagattg ggacatacta tatcgaagtg
    34141 cactgcgatg gctatgtctt tccgtctgac gacagcgtaa cgcttgatgt tagacgttct
    34201 gggcaaaaat atgtcgttag cactgattta atcacagaca caactatcca aaaactatca
    34261 gctgatattg agtatttaaa atcaaaagtg actcaaaatc agcatctatt tgagcaagtg
    34321 tcaccacaaa cagaatggac cattacacat aatttgatta agtatccatc tgtgacaatt
    34381 gttgatagtg caggtaatga ggtttttgga agtgtcgagt acattagcac aacaaaaatc
    34441 atcgttagat tctcagcgcc atttgctggc aaagccattt taaattaaag gagattacat
    34501 gagtacattt ttaacaaata tcaaccttaa taaaaacgag ttgcaaaatg cagtcgtaca
    34561 aaatttaggc tcagagccac aagatccaaa aacagggcaa ttttattatg acagtgttgc
    34621 taataagcta aaaatttata acgggacatc ttgggttgct ttagacaagg aaacagcaga
    34681 aagcattgtt aaccttatta attctggtga atccaaaatc gataaatcaa aagtcgatgg
    34741 acttgaagaa gcaattaatc gagcgtctat gactggtcaa gctatcgcga ccgctttaaa
    34801 cgaagatact ggtggcgtta ctgtaaacca agataaaatt acagggctta cagataaact
    34861 agctgaaatt gcgactaaca aagcgtcagc gactcgggag tcacaagatg ctttgcaaga
    34921 agccaaggtg tatgctgacc aaaaagtagc cggacttgtt gatagtgcat cagaagatta
    34981 caacacgttt aaagagctag aaacttttat taaacaaaac gcgaaaggtc tcgcagcaat
    35041 ctcaaatgta gccaaaaaat ttagtaaaac gtttggcgat gcacgagcta aagaatttac
    35101 aatcacacac aacctaaaaa ctcgtgatgt taacgtcact atccgagaga ctaacttccc
    35161 ttatgcaatc gtctacgcag atattaaagt cacagacgaa aatagcatct taatcgcaac
    35221 aggtgcagaa gtaccaacgc aaaatcagta tactgtgaca attactggat aggaggccgc
    35281 tatgagatat attggcgaat cacaactctc aaaagacctt gtaacaaaag attatgtaga
    35341 taacaaagtg actaatctcg cgagtaagga tgatattgtt aatttaggta aaattaagga
    35401 ctcgcaaaca ggtgcgtttt tggaagtcaa aatagtccca aacggtcaag tcccttacga
    35461 caccactggc atgattgtgt ttgaacgctc tggaggtaag taatgtcagt acaagagata
    35521 ccagatgaaa ttttttatcg attaatagag caaacagtag atacaaaact ggtttgggat
    35581 aaggacacac aaataggtag ctattatata tctatacacg ataagtatag ttttgaatct
    35641 ggtagagatg ttaacatcta taaaatctta tctagcacac aggctgatgt gtataaagct
    35701 aataagtata attatcaatc atcatcaaaa ccagatacta gcgtaccaca gccgaagcca
    35761 ccagagcaac ctccgctcaa aaacaagtct ttgtatcaat caatcacgat tgatggactg
    35821 ccaatagctc gcgttatctg taacaatcat gttatctggt ctcaatcagt agagcaacaa
    35881 gaagtctaca acattacagc gtacgtagat gctagcgtat ctaagctaaa tgactctgcc
    35941 attattgatt acaatcgaga ttttgcggct tacggcgtaa attggagtcg agttgataga
    36001 gtcgccgttg aagtcgacga taaaatagtt gctaagctac aagctcctgt ctatgatttt
    36061 ggtgttagag gtggcacgac gatgtctgtc gaaaaatggg attacccatc aaatacaata
    36121 gaaaaataca aaaatccaac atttaaaatt tcgcagcatt aggaggtaaa gctccgagat
    36181 aagacaaaac cgctcagatt attctgggcg gttttttgtg ttataagtaa ttattttttt
    36241 aatttttttc tttcattttc aatttctatt tctcgtttta ttaacctatg aaggaggaat
    36301 ggaagtagtg ctaaaataga acaacctgta agaaatattg cacttacttt gtcagaacga
    36361 ttagttgata aaatggtcga taaa
//
LOCUS       DAUELZ010000008        28403 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.008, whole genome shotgun sequence.
ACCESSION   DAUELZ010000008 DAUELZ010000000
VERSION     DAUELZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28403
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.008"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(356..1762)
                     /gene="lacG"
                     /locus_tag="VHI09_000286"
     CDS             complement(356..1762)
                     /gene="lacG"
                     /locus_tag="VHI09_000286"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEQ6120193.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGDVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDGGYSGEIGVVHAL
                     PTKYPFDANNPDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFVALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFMWSLMDVFSWSNGYEKLYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(1850..3547)
                     /locus_tag="VHI09_000287"
     CDS             complement(1850..3547)
                     /locus_tag="VHI09_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ6120194.1"
                     /translation="MNGLIAQIEKGKPFFEKISRNIYFRAIRDGFITGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFMSTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKTCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKVYDEQILTEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKAQFEN"
     gene            complement(3547..3864)
                     /locus_tag="VHI09_000288"
     CDS             complement(3547..3864)
                     /locus_tag="VHI09_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ6120195.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(3888..4871)
                     /gene="lacD"
                     /locus_tag="VHI09_000289"
     CDS             complement(3888..4871)
                     /gene="lacD"
                     /locus_tag="VHI09_000289"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ6120196.1"
                     /translation="MTITLTENKRKSMEKLSIDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEAGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDVNEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKIADNASPEFAKVKAHKVNEAMKVFSKERFGVDVLKVEVPVNMKFVEG
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLEFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            complement(4875..5804)
                     /locus_tag="VHI09_000290"
     CDS             complement(4875..5804)
                     /locus_tag="VHI09_000290"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEQ6120197.1"
                     /translation="MILTVTLNPAIDVSYPLDELKCDTVNRVVDVTKTPGDKGLNVCR
                     VLNDFGETVKATGCIGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPLLSVEEIDGFTHHFKYLLNDVDVVTLSGSLPAGMPDDYYQKLIGIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFEALKEVLQDELFEGIEWI
                     IVSLGADGVFAKHNDTFYNVDIPKIEIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            complement(5852..6367)
                     /gene="lacB"
                     /locus_tag="VHI09_000291"
     CDS             complement(5852..6367)
                     /gene="lacB"
                     /locus_tag="VHI09_000291"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ6120198.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKTQGHEIIDCGTYDNVRTHYPI
                     FGKKVGEAVASGEAELGVVICGTGVGITNAVNKVPGIRAALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWVRGEYHD"
     gene            complement(6402..6830)
                     /gene="lacA"
                     /locus_tag="VHI09_000292"
     CDS             complement(6402..6830)
                     /gene="lacA"
                     /locus_tag="VHI09_000292"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ6120199.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKYEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNSRII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
     gene            7276..8049
                     /locus_tag="VHI09_000293"
     CDS             7276..8049
                     /locus_tag="VHI09_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ6120200.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTGIIEKYKKFSKIIIADK"
     gene            8699..8986
                     /locus_tag="VHI09_000294"
     CDS             8699..8986
                     /locus_tag="VHI09_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible protein J"
                     /protein_id="HEQ6120201.1"
                     /translation="MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNV
                     VVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            8976..9311
                     /locus_tag="VHI09_000295"
     CDS             8976..9311
                     /locus_tag="VHI09_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ6120202.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFPEVGFDADEKYGSKISHYHSTKGYTLSKDYIVLYHIEGEENRVAIDYLLPT
                     QSDYIKLFK"
     gene            9450..9626
                     /locus_tag="VHI09_000296"
                     /pseudo
     CDS             9450..9626
                     /locus_tag="VHI09_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(9757..10149)
                     /gene="rpsI"
                     /locus_tag="VHI09_000297"
     CDS             complement(9757..10149)
                     /gene="rpsI"
                     /locus_tag="VHI09_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEQ6120203.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10170..10616)
                     /gene="rplM"
                     /locus_tag="VHI09_000298"
     CDS             complement(10170..10616)
                     /gene="rplM"
                     /locus_tag="VHI09_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEQ6120204.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(10834..11040)
                     /locus_tag="VHI09_000299"
     CDS             complement(10834..11040)
                     /locus_tag="VHI09_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6120205.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEADDSH"
     gene            complement(11037..11735)
                     /locus_tag="VHI09_000300"
     CDS             complement(11037..11735)
                     /locus_tag="VHI09_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120206.1"
                     /translation="MFDSKQNLAKSQWMFIIITAVMDLVVLVATLLAKTSFQRYLVGG
                     VAVILTSFLILLVWGLKTAKKLQTRLDSKEILDSKVRDDQKLPRYDERQKQILLKGYT
                     IGFWFMIVAVWLSIFLHRFSERLVSASFLFTLALWGGLAVQTTYCNLNGVSPFVDRRF
                     GKQGILVGMIATGMSLVTIGLAVREMMAGQLKMGSFFKAGGSGSLMVLGITLLSMGAS
                     IFYRHYLDAKEADE"
     gene            complement(11871..12731)
                     /locus_tag="VHI09_000301"
     CDS             complement(11871..12731)
                     /locus_tag="VHI09_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ6120207.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHAKNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(12828..13346)
                     /locus_tag="VHI09_000302"
     CDS             complement(12828..13346)
                     /locus_tag="VHI09_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEQ6120208.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKTNQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     SQLKDFMSQLDR"
     gene            complement(13350..14096)
                     /gene="rlmB"
                     /locus_tag="VHI09_000303"
     CDS             complement(13350..14096)
                     /gene="rlmB"
                     /locus_tag="VHI09_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEQ6120209.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKARGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            complement(14143..14940)
                     /locus_tag="VHI09_000304"
     CDS             complement(14143..14940)
                     /locus_tag="VHI09_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120210.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASMRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAM
                     TNSVDVDTKAQTNQSANQEIDSTSNPFKSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLSGLVVSLAAGLFIAIQKVSRRK"
     gene            complement(15136..15549)
                     /locus_tag="VHI09_000305"
     CDS             complement(15136..15549)
                     /locus_tag="VHI09_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEQ6120211.1"
                     /translation="MTNPVDVNLINGIALAFEGDAVYSCYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            complement(15542..16885)
                     /locus_tag="VHI09_000306"
     CDS             complement(15542..16885)
                     /locus_tag="VHI09_000306"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEQ6120212.1"
                     /translation="MIKIYDTMTRSLHKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKAIHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFATADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            complement(16913..17143)
                     /locus_tag="VHI09_000307"
     CDS             complement(16913..17143)
                     /locus_tag="VHI09_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120213.1"
                     /translation="MNKIRVGLYVIMSLSILVSSITLVASLIFLLKQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            complement(17486..18067)
                     /gene="cysE"
                     /locus_tag="VHI09_000308"
     CDS             complement(17486..18067)
                     /gene="cysE"
                     /locus_tag="VHI09_000308"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEQ6120214.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTIRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            complement(18076..18828)
                     /locus_tag="VHI09_000309"
     CDS             complement(18076..18828)
                     /locus_tag="VHI09_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEQ6120215.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSLALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMARFKEEQKSAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDDNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            complement(18821..20953)
                     /gene="pnp"
                     /locus_tag="VHI09_000310"
     CDS             complement(18821..20953)
                     /gene="pnp"
                     /locus_tag="VHI09_000310"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEQ6120216.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPVAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAIDRTKEIIAGLVREAKV
                     GEVYHAKVIRIEKFGAFVNLFDKTDALVHISEIAWTRTANVSDVLEVGEDVDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            complement(21233..21964)
                     /locus_tag="VHI09_000311"
     CDS             complement(21233..21964)
                     /locus_tag="VHI09_000311"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEQ6120217.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPLIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTAGVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEEVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLEAVQMFLKDSTSLGFKILEDAKH"
     gene            complement(21974..23353)
                     /locus_tag="VHI09_000312"
     CDS             complement(21974..23353)
                     /locus_tag="VHI09_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEQ6120218.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVANLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLAFARFTRFKYIFLTGHHSFFMACLLSAVLGAVGFKGSLLIILGGFLLGAWS
                     AISPAIGRQYTLKVTDGDEIAMGHFGSLGYYLSAWFGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVIFYLVATVASVLRNASVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSEEI"
     gene            complement(23380..23664)
                     /locus_tag="VHI09_000313"
     CDS             complement(23380..23664)
                     /locus_tag="VHI09_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ6120219.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            complement(23657..25717)
                     /locus_tag="VHI09_000314"
     CDS             complement(23657..25717)
                     /locus_tag="VHI09_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ6120220.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESMLKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVTSGPWVFSYLLHELGLPDQTKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEAVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKLVNEDEVAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     AKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLIGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLTPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            complement(26069..26338)
                     /gene="rpsO"
                     /locus_tag="VHI09_000315"
     CDS             complement(26069..26338)
                     /gene="rpsO"
                     /locus_tag="VHI09_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEQ6120221.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNNHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(26497..26652)
                     /locus_tag="VHI09_000316"
     CDS             complement(26497..26652)
                     /locus_tag="VHI09_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120222.1"
                     /translation="MTMLGYRLSAEVRGSAILPLLAGYLIRQAIAQVYSDLKTASWLF
                     GLASSVA"
     gene            26778..27035
                     /locus_tag="VHI09_000317"
     CDS             26778..27035
                     /locus_tag="VHI09_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ6120223.1"
                     /translation="MTQNVCQQNITYTVKERFASYILEAQANQEVHLNFTLLANRFGI
                     SDRHLKHVLKQLIFQRIIERICFKTCRIADLDALTQLSVNN"
     gene            27162..27776
                     /locus_tag="VHI09_000318"
     CDS             27162..27776
                     /locus_tag="VHI09_000318"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ6120224.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(27810..28352)
                     /locus_tag="VHI09_000319"
     CDS             complement(27810..28352)
                     /locus_tag="VHI09_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ6120225.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVAYLNWKDVPV
                     LNQDIEANAPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETKANLLSQAEALLAAI"
BASE COUNT         8628 a         5970 c         5081 g         8724 t
ORIGIN      
        1 taacaaatag ccctctgata taaaaaacaa aactcgttct ttacagctat tttacaatct
       61 aatcgcttct cgttataaca tcaatctcta atcagaaaat ggttacttta cttatttaat
      121 caggtcagga gtttgaatga cagaagtcaa atatctgaaa aaggatgtta aataaaactt
      181 tcaaaaccca ttttcatcaa ctaaaaacac ttataacccg atcttacaac taatggatta
      241 ccatcaacag caaataatac tatgcttgaa aaatgtcaac atcatctact ttttgtgact
      301 ataattcagt aaacgaacat gacgtgtaaa aatgtattaa agcagtatct caaaatcatt
      361 caatcacttg agtttctgct acttttttat accagtaggc actcttctta ggataacgtt
      421 cttgagtttc aaaatcaaca tagaagagac cgtaaagttt ttcatagcca tttgaccatg
      481 aaaagacatc catcagtgac cacataaagt atcctttaac atttgcacca tcagaaatag
      541 catcagaaat aacttctaag tgttttttca cataatcgat acgtccatca tcatagacag
      601 tattatctac aaactcatct ttgtagccaa gaccattctc tgtaatgtaa attttcttgt
      661 aattaggata atcggctttg acacgcatga tttgatcata taagccttgt gggaagataa
      721 tccagtccca gtccgttttt ggaacatcaa cgggtgcttt tcttcgacca acacccttga
      781 tttggtattt agagctgcct ttttcaccct taccattgtg aatgatttca gtctcaccat
      841 caaaagcttg catccaatca ctcatatagt agttaatacc aaggaagtca ttcaaatctt
      901 ttgcagcatc cagtgctaca aagtcttctt cacgaagatc aagttcacca ccattcacct
      961 caaggatatg gttaacacct tccattgttt tatctgaata cttacctaga taagttgcat
     1021 caagaataaa tttattatgg atgatatctt ccagttcagc tgctctaaca tcatcaggat
     1081 tgttagcgtc aaatggatac ttagttggaa gagcatggac aacaccaatt tcacctgaat
     1141 agccaccatc tttaaagagt ttcacagcgc gagcatgaga aaccatcatg ttatggtgtg
     1201 attggaaaac tttagcaaga tcatattgaa tacctggagg gaatttacca actaagtatt
     1261 ggccatcacc aattggtcca atttcgttaa atgttgtcca atagttaact tctgagaatt
     1321 ctttaaaaca gaattctgca taatttacaa aatgttcaat gttctcacga ttgaggaaat
     1381 caccatccga gtggagagct tctggtgtat caaaatggtg aagtgtaaca aaaggctcaa
     1441 catgacgctt atgacactct gcaaagagat tgtggtagta ttcaactcct ttagggttaa
     1501 cgtctccttt tcctgttgga aaaatacgag accaagcaat agagatacgg atgccgttga
     1561 caccaaattc ttcactaagt ttcaaatcga ctggataacg attgtaaaaa tcacttgctg
     1621 gttcagcagt gtaccaatag ttgtcttcta agtatttatc ccaagctact ggtcctttgc
     1681 catctgtatg ggtagcacct tcagcctggt aagcagctgt agcaccacca aaaataaaat
     1741 ctttaggtaa tgtttttgtc atctcagttt ctccttgaat atctttcaaa aataaagttc
     1801 ccaaaacaat ttttgtgttt gagaacttta cgacatttaa actgttttct taattttcaa
     1861 actgtgcttt tacaaaatca agagctcctt gaccatcacg ggtcaatttg atgtattgag
     1921 ccccttctgt tttagctaat ttaatatcaa gtttatcagt ttccactttc atatcttcat
     1981 agttagaagc tacttgaggt gcaaggataa caagttgata ttgtggtaaa atctcacggt
     2041 gagcaccata acttcctgct gctgcagtga caggtgcacc atattcttta gctgctttat
     2101 tcaaagcatt tgctagtagt ccacttgtac caccaccggc acaaagaacg agaacattag
     2161 tttgatcagt gatttctttg gtttctttag ctgctgattt ttcaagaata gcatcagctt
     2221 ttttagtatc aaagtttgct gctacttttt ctttgagggc atctgatgaa ttcgtacctg
     2281 ctgcttcttc agtaagaatt tgctcatcat atacttttaa gaatgggtag tagatgatca
     2341 catcaacgac aattaacaaa gctgccaaaa tgaatgctag cacttggaag tttgtcccaa
     2401 ggataagacc caatggagct ggtgttgtcc aaggtaagtt tgcagtgaaa ctgttcattc
     2461 caagtgtgtc aatgaagaat ttgaaaatcc atacgttgac aatcggtgcc aagataaatg
     2521 gaataaagaa aattgggttc aaaacaagtg gggcaccgaa caagataggt tcgttaacac
     2581 caaagaatgt tggaacaaca gatgcacgtc caatggcttt gttacgtttt gattttgtta
     2641 accacatgaa catgaatgga acgacgagag ttgcaccagt accacccatg gtaacgatga
     2701 acatttgagt ccctgaagtc aaaatcttgt cagcatgttc accagcttga agtaattgaa
     2761 ggttggtttc agcatttgcg taagtaatcg ctgcaatggc tggttcaaca atagatggtc
     2821 catgaatacc aacaaaccag aagaaagcaa atgcaccgaa gataatagtg attccaacat
     2881 aaccatctgc tgcttggaac aatggtgcaa gaagtttacc aattgactcg gcaacgttaa
     2941 cgcctaaagt agtatgaaca atcaattcaa tcacataaag cagtacaact gataacgtaa
     3001 atggaatcaa atctttaaaa acttgagata tatttggtgg aacctcttct ggcatgcgaa
     3061 tagtaacatt gtttttcaca caggttttat agacatttac ggtaataaaa gctgcgatga
     3121 aggctgtcaa caaacctttt gttcccataa atccagttaa gaaaccaccg tcttttgctg
     3181 gattggctgc cataagtaaa aaacctacaa tagcagcgag catggtagac atgaagttga
     3241 tttggtttgt gcttggtaat gatcgattca ttgagtcagt taaagctttg gctgtcgtac
     3301 cagccacaaa caaacctaaa atacccattg agtagttgta tggcgtcatc aagagattct
     3361 caatatcctt actccaatgg aagccccatg catttggaac ataggcaatc aagataaaga
     3421 tacttgaaaa caagataact ggcataccag tgataaaacc atcacgaata gcgcgaaaat
     3481 agatgtttcg agaaattttc tcgaagaaag gtttcccttt ctcaatctga gcaattaacc
     3541 cattcattat tttgctcctc tcttataaag ttcaatcatg tgtttcatta aatcttgtaa
     3601 taaaatggtc gtcattaaat gatcttgacc atgcattaaa gtgacactat aagctaagtc
     3661 atctccagct gcttcttttt gtagtagact tgtctgagca ttgtgagcat cattcaagca
     3721 gtcactacct gcagcgatta attcttctgc tttagcataa tctccttctt gcgctgcttt
     3781 aagcgcttcc aaaaattttg aacgggcatc accagcatag gctacgattt caaaacctaa
     3841 aagagtagtt tcttctctat tcattgtagt ttcctccgaa aataatgtta cattttttca
     3901 gtccaaggac ttgcagtttt atctagtact ttgtttaatt cgtcaatatt tttgaaacct
     3961 tctgtacgca accattcacg agctgcttgt ggtccttctt cgatatagac ttttacagat
     4021 cctgcccagg tagcacgtcc acataagaca ccgttgaatt tagcacctga ttctgctgca
     4081 aattccaaag tatcttggaa caacttggct gaaacacctg cactcaagta aatatatggt
     4141 aaatcagtag aagcttcttg atcacggaaa gcttgagctg cttcttcttt tgtaaagaga
     4201 acctcaccat ctgcaaagcc ttctacaaat ttcatattta ctggaacttc aactttcaac
     4261 acgtcaacac caaagcgctc ttttgagaaa actttcattg cttcgttaac tttatgagct
     4321 ttaacttttg caaactcagg gcttgcattg tcagcaattt tctcatcata agttagaatt
     4381 tctaagtaga atggaatatc ttctgcacgg cattcagaac caatacgttc aatgtaagct
     4441 tttttctgct cgttaacatc ttgatctcca tcaatatcat agtaaagaag gaatttaact
     4501 gcttctgctc cagcttcttt aatacgtttt gcagaccaaa catctaagca atcaggtaag
     4561 cgactagttg ttgttgcatc gtatcctgtt ttctcatatg ccaacaacaa gcctgcttct
     4621 tctgaacgaa cacgacttgc tggcaagcca tattcaggat caagtaaaat tgatgatgca
     4681 aatggtgtca gttcttcaga aactaaactt ttaagctctt caatttgttc tactgttggc
     4741 tcttttgttt gatgttgagc catcatacgt ttgagagctc cacgttggtc aaaagctaaa
     4801 gcagaaataa caccatctat acttagtttt tccatgcttt tacgtttgtt ttcagttaaa
     4861 gtaattgtca ttttttatac ctctttgact ttaatagatt gatatagctt atcgtaattt
     4921 gccatgttaa catgcccagt tgttttttct tgcgcattta acatccctaa gacatttgct
     4981 tttgttaata aagctcgatc atcttcatca ttcgctaaac ctgaagcaat acctgctact
     5041 gtagaatcac cagaacctac agcgcttaca atttcgattt tagggatgtc aacgttataa
     5101 aacgtatcat tgtgctttgc aaatacacca tcagcaccaa gagaaacgat tatccattca
     5161 attccttcaa atagttcatc ttgtaagact tcttttaaag cttcaaaatc ttttgtcatt
     5221 tctttaccta gaagttgaga aagttcttca agattaggtt tgataactgt tggtttacta
     5281 tcacctttta gtactgcttc tagagcattt ccagaacaat ccagaaccgt tttcttacca
     5341 ttaagattgg caataccgat taacttctga tagtaatcat ctggcatacc agcaggcaaa
     5401 ctgcctgaga gcgtcactac gtcaacatca ttcagtaaat atttaaaatg atgagtaaat
     5461 ccatcaattt cctctacact taacagcggt cctttttcta aaatctccgt ttgattacct
     5521 tcgtgtaaaa tggctataca agttctagta tcacctgaaa ttttatagaa gcgacttaaa
     5581 atcgaatcag gaagatgatt aataataaaa tcaccgcttt ctccgccaat acaaccagta
     5641 gctttgactg tctcaccaaa atcatttaga actctgcaaa cattcaagcc tttatcaccg
     5701 ggtgtctttg taacgtctac aactcggttt acagtatcgc attttagctc atccaaaggg
     5761 taggagacgt caatagctgg atttaaagta actgttaaaa tcacaatagc tcctttttct
     5821 ctatatatcc ctatagtaat ttattcaaat attagtcgtg atattcacca cggacccatt
     5881 tttctagaaa ttcatcaaag aagtgtgggt tgttttgatc ttgactagca tgttgcaagc
     5941 tatccatttt ttcaatccat tttttatttt cttcagttgg tttgtattcc gcggctaaga
     6001 aagcatcgat aatatcattc atcaataaac caccgataat ttttccacca aaaccaataa
     6061 cgtttgcgtt tagctcttct tttgagtaga tagcagatgt catatctcta acgagtgctg
     6121 cacgaatacc gggaactttg tttacagcat ttgtgatacc aacaccagta ccacagataa
     6181 caacacctaa ttctgcttca ccacttgcta ctgcttcacc aactttttta ccaaagattg
     6241 gatagtgtgt gcggacatta tcgtatgtcc cgcaatcaat aatttcatga ccttgtgttt
     6301 tcaaatagtc aacaacagcg attttttcat aagtaacaat atggtcacaa cctacagcaa
     6361 ttttcatttt tgttctcctt gttttaacct atttcaatca attagcacat tttattaagc
     6421 atgtcgacac gaacttgatg acgtccacca tcatatttac cttcgacaaa gcctttgata
     6481 atgttctttg caattcctgg agcactaatt tcagaaccaa gcgtgatgat tcgagagtta
     6541 ttgtgacctc ttgtcatgta tgctgagcgc tcatctgata cttcagctgc caccattcct
     6601 ttgattttag tagctaccat gaaagaacca acaccatatg catcaatgac aatacctaag
     6661 ttatcttcag atgcattaac ttcttttgca acggctaaag ttgtatctac aaagtcacgt
     6721 acttcgtttt cactaacatc aacgacttcg tatttacctt ctttaaggta atctttgata
     6781 acttctttaa gttcttgacc tgctttgtca gcaccgataa taattgccat tttatttctc
     6841 ctataattta gaataagtaa atgtggaaaa cttacgtaga tagttaatta acgatttgca
     6901 aaatcttttg aaataacgct ttcattattt ctatgaccta atcatacacc caccaaacat
     6961 aaaagtcaac atttttttgt ttattcttat aaaaaataaa caaaatatgt ttattttttt
     7021 catttttacg ttattatcct aatatttctt gtgtttcttt ttcatttcat aaatcccttt
     7081 gacagccact cgtaacctat cctatattac gaaaccctta tctctattta ttgtaaacgt
     7141 tttatttaaa acgttttcta ttttgttaat ttttgttttt tccaacattt atgtttgttt
     7201 tgtttgattt ttttattgat attatgttat gataccttta atagttatac cctaagacac
     7261 tgataggaga taagaatgaa aaagaaagag cgtcatgaaa aaattctcga tatcttaaaa
     7321 gttgacggtt ttataaaagt aaaagatatt atcgatgaaa tgaacatctc agatatgacc
     7381 gctagacgag atctcgatac attggcagat aaaggattac ttatcaggac ccatggtggt
     7441 gctcaatatt tggattattc ttcagcaaag gacgaaggtc acgagaaaac acacactgaa
     7501 aagaaggtct tacagactac cgaaaaaaaa caaattgcac aaagagctaa aaacattatt
     7561 aaagatggcg aaactgtctt tataggtcca gggacaacat tagagcatct agctgtcgaa
     7621 ttgaaaaata gaaatatcag ggtcattaca aatagccttc ctgttttttt aattttgcac
     7681 ccgagcacta ctattgattt gattttaatt ggtggtgaat gtcgtgaaat tactggagct
     7741 tttgttggtg ctattgccat taataatcta gaatcattaa ctttttcaaa ggcttttgtt
     7801 agcgcaaatg cagtttacaa taattctatt gcgacgtaca gtgatctcga aggagaagtt
     7861 caaagggttg cattgaataa tgctgttgag aaaatccttc tagttgatag taccaagttt
     7921 gaaagttatg acttctataa tttctaccaa ctagatcaag tggacttaat tgtgactgat
     7981 aaaaatgtga caactggtat catagaaaag tacaagaaat tctcaaaaat cataatcgct
     8041 gacaagtaaa ggacctactc atattaaaaa gcacttcagg gtttagatat atcccctgtc
     8101 aagtaaacag ggagcatatc tgaaagtagt gtttgtatta taaagaagtt tcttgctaac
     8161 ttattttctg gtttttttca gacttatcag tcttgtactg ttcctttttc tgatgaataa
     8221 cgtggaaata ggcagacagc tatatatttc acctctagaa tggttgccat tactgtcatc
     8281 aagacatgct tgttctttat cgtatggagt aaagctttct tttagccatc aacatacctc
     8341 ttaagtagat tttggttcgt taccatgtct acttaagagg tgtcttcaaa gcgctttaaa
     8401 acgttgatat aacagtatat taacgttttg agaattgtga agctttacga gcttttttaa
     8461 gacctggttt ttggttcact ccattattta aagtcaaatc cctttaaaat caatgtttct
     8521 aaaatgaaaa atcactaaaa atcttattta tagacaaata cttaaaccta aacggtatca
     8581 aaaataagtt tcggagctta tcaaataatg aaaatctgtc tcaattaccg agtctttttt
     8641 attgactttt ttgtatatat gatataatta aataaattac ataaaggggt tacataaaat
     8701 gactacagta aaaaaaaaca gagcggttac ctttcaagct aataaagaat tggtaagcga
     8761 agcaatgaca gtattaaaca aaaaaaattt aaccttatca tctgctttaa gattattcct
     8821 acaaaatgtc gttgtcacaa acgaggttga cttattgacg gaagaggagt tagagaaaga
     8881 aaaacttttc aagcaatttc aagctgaaat caacaaaaat attgaggacg ttcgtcaagg
     8941 gaagttttat acctctgaag aagtgagggc tgaacttgga ctataagaaa tatcagatta
     9001 tctatgctcc tgatgtttta gagaagctaa aagaaattcg tgattatatt tctcaaaact
     9061 actcctcaac atcaggtcag cgtaaaatgg agcaaatcat tagcgacata gaaaaactcg
     9121 aggttttccc agaagttggt tttgatgccg atgaaaaata tggttcaaaa atcagccatt
     9181 atcacagcac taaaggttac accttaagta aagattatat tgtcctatac catatcgagg
     9241 gggaagaaaa tagggtcgct attgattact tgcttcctac tcaaagcgac tatataaagt
     9301 tattcaaata acacgttaaa agccttagca gtcttgctag ggcttttttg gtagattacc
     9361 aattaactag gagcaaaatg agttcgggct ggttaataga tattccacaa agaagccaaa
     9421 gactacgaag ctaggctaat ctaccttaag gcttcaggca tacccatgca accctgatga
     9481 ttgagattgg agttgaccca gtaaatacag ccaaaagact aggacatgct agtagtcaaa
     9541 tgactcttga tacatacagt catgctactg cagtcggaga ggacaaatca atcaaacaat
     9601 ttgctaacta tctcaaaaat aactaaataa acgtattaaa ccaagtcaaa agttttccga
     9661 aacggtatca aaaaaggtat caaaagcaaa aaacgacaaa aaagcacctt gcaagagact
     9721 tgcaaaatgc ttgtaatcgt tgatgtaaag ctgttcttaa cgttttgaga attgtgaagc
     9781 tttacgagct ttcttaagac ctggtttttt acgttcaacc atacgtgcgt cacgtgtaag
     9841 aaggccagcg cgtttcaatg aatcgcggaa gtctgggtct acttgaagaa gcgcacgagc
     9901 gataccatga cggatcgcac ctgattgtcc accgtaacca ccaccaacaa cgttaacgaa
     9961 aacgtcgtat gaaccttcag ttgatgtaac tgcaaaaggt tggttgatga tcaaacgtag
    10021 gtcagcatgt gggatgtatt cttctacatc ttttttgtta acagtgattt taccagtacc
    10081 tggaaccaaa cgaacgcgtg caacagcgtt tttacgacga ccagtacctg catattgtgc
    10141 ttgtgccatt atttaatgct cctttcctct tagataagtc ctgagatgtc aagtacttct
    10201 ggttgttgtg ctgcgtgagt gtgctcaccg ccaacgaaga ctttcaattt cataccttgt
    10261 gcacgtccaa gagtgttatg tggaagcatg cctttaactg atttttcaat caagcgaact
    10321 gcgtttttag agcgaagttc accagcagtg attgatttca aaccacctgg gtacattgag
    10381 tgagtgtagt aaactttatc agtcgctttt ttaccagtta atttaacttt ttcagcgttg
    10441 ataacgataa caaaatcacc tgtatcagtg tgtggtgtga aagttggttt gttttttccg
    10501 cgaagtacgc tagcaactac tgcagaaaga cgtccaagtg gcacatcagt tgcgtcaaca
    10561 acgtaccatt tgcgttcaac ttggcctggt ttagccatga aagttgtttt gttcatgatt
    10621 tctcctatac gaattcgtaa tatttgttta caaggtgatg ggtgttccgt cccatcgaag
    10681 gtatttggaa ggttccgggg cctttcaaat ggggtaaaca ataccgccta ctatgatatc
    10741 aaacttaaga ggctccagtc aagtgcttta gctgatttta ttggattatt ttttctttcg
    10801 tttttgagga atggcagagc tgctctgctg atgttaatgg gaatcatcag cttcccaaaa
    10861 aaggtcgtct aaggtcttat caagcactcg acaaatggca atgcaaagat taatcgttgg
    10921 gttgtaatca cctttttcaa cagccgcaat ggtttgtctg gagacgccta ccaaatctgc
    10981 tagagcttgt tgagacaaat ctttgcctgc acgcgctgct tttaatttga ggttcttcat
    11041 tcatctgcct ctttagcatc cagataatga cgatagaaaa tactagctcc catactaagg
    11101 agcgttatgc ccaacaccat taaagatcca ctgccgccag ccttgaaaaa gcttcccatt
    11161 ttaagttgcc ctgccatcat ctctcttacc gccaaaccta tagtgactaa actcattcct
    11221 gttgctatca tccccaccag tattccttgt tttccaaagc gcctatccac aaatggcgat
    11281 accccgttga ggttacaata tgtcgtttga actgccaacc ctccccaaag agccaatgta
    11341 aagaggaagc ttgcagatac cagtctctct gagaagcgat gaagaaagat tgacagccaa
    11401 acagccacaa tcataaacca aaacccaata gtgtatcctt tgagaagaat ctgtttttgg
    11461 cgttcgtcat atctgggcag cttctgatcg tcccttacct tagaatctag tatttctttg
    11521 ctatctagtc tcgtttggag cttttttgct gtctttagcc cccataccaa caatatgaga
    11581 aagctagtca atataaccgc tacacctcca actaagtaac gttgaaaact ggttttagcc
    11641 agcagtgtcg ctactaacac aaccaaatcc atcactgctg ttatgattat aaacatccat
    11701 tgtgacttag cgagattttg tttgctatca aacatgattc gtttctcctt taaatcttta
    11761 tatgacataa tataaattat tttagatatt ttgtcaaata tattttacat tcttgattga
    11821 tttaagcagc aaaaaaggat tgaggatttc tcttcaatcc ttataaggga ttaacgggaa
    11881 ttctgcccaa tcacaaaaac tgccagggtg ttaggaccaa cgtgagctga gataactggt
    11941 cctagtggca tcataagaac atcgctaata ttctcatgcg ccagcagctc ttctcgcaac
    12001 ttttcggcac taccctgatc gcttgtataa gagacaataa ccgtcgaatc cgcaatatct
    12061 ttttccactt gagccaccat ttctttgatg gctttttggc gtccccgaat ttttgcaatg
    12121 ggcaccaatt tcccttcctc atcaatccac aaaagaggtt tgatactggc taaactgcct
    12181 agaaaagctg aaccttttga cagacgccca ccacgcatca aatgaaaaag atcatctact
    12241 aagaaatagg tgcgcagtcg aggaatcact gcttcaacaa tatctttagt ctccagtaaa
    12301 tttttgccac tatctctggc ttctgctgct aaaatggtta gatagccttc tcctcctgca
    12361 gcagccaagg tatcaacaat ttcaatcact gcatcgggat aatcttctcg aacaagatcg
    12421 cgtgccatta gagcgctttg ataggtaccc gataaaaccg aagaaaaagc aagataaagc
    12481 agtgctttgt tgttcttagc atgttcacga aaaaccttct caaactctcc cacattaatc
    12541 tgactggttt gaggatgact gccagctttc atctttttca aaaggtaatc actactaata
    12601 cggttaggac cgaccgtttc ataaacttct ccatcgcaca aaatcgttaa tcctataagg
    12661 acaatatcat gatcttctgc ccaggtttga ttcaaatcag cggttgaatc tgtcattatt
    12721 gtaaaggtca ttcttacttc tcctcatcca gttttttagt ttgacaatca aataattatt
    12781 gttttatttt atcaaaaaaa cccgcacttg gcgagtcgta attctcatta tcgatctaac
    12841 tgagacataa aatccttcag ttgactaagc tgtccttggt caaaaggccg cagtttaggc
    12901 gttttgagat caatatctct agacatttta tctaaatcag ctttgacagt atgaactctt
    12961 tgctctaatt ctcttgcagt gacacgaaga gctccttgag aaaaaatcgt ccattgttca
    13021 tttaaatcac tcgtagctac ttctaccata tgtatagccg tatttaactc tgctgccatg
    13081 cgctcaatgt agctatctgc tgtttcgtct tcctctgtaa ataccactga gatataatac
    13141 tggtcatatc gttgccttaa ccccggtaca tattgggcat caaaaacaca aataatatta
    13201 atattctcaa aatgggcata atgattaagt tttgttaaaa gtgtgttacg tgcctgatca
    13261 agctgatttg tcttaaacaa ctgacgggtt gattgccaaa aggcaatcat attataccca
    13321 tctactaata atatccgttt tttcataatt tagcgcctat ttcggaaaac ttcatacatg
    13381 agaatagctg ccgctacgct agcatttaag ctctgtacat gaccattcat aggaatcgta
    13441 atcatttcat caacttgctt tttgatattg gtagagatgc ctttgccttc gttaccaatg
    13501 actaaagcaa gtttaccatt agtgttccag caatcagacg gtgttccatt catgtctgtg
    13561 ccaaaaatcc agaatcctct tgctttcaat ttatctagag tttgactaag gttagttact
    13621 ctcgctatcg gaatatgttc aacagcacct gtagacgtct ttgagaccac tggagtcaca
    13681 ccaacagaac gatgtttagg aataatcact ccacatacat ttgtagcatc agctgtccgt
    13741 aaaatcgatc ctaaattatg aggatctgtc agaccatcta aaataagaat caaaggattt
    13801 gcttcttgct ctgctatttc gaggatctca tcaacatcag tataggcaaa agctgatact
    13861 ctcagaacaa aaccttgatg cacagctcca tcagtcattt gtgacaaggt ttttttaggc
    13921 gtccatgaaa tagcgacctt tttttgtgtc gctaagcttt tgatgttatc cactttcttt
    13981 cctcttaaat cctcttggat ataaagctta tttcctgtat ttgcttgaag gctttctgta
    14041 acggcatgaa caccatagac gatatcgttt gtttcaatag tatctttatc ttccatgagt
    14101 ttattatacc atgaatgctt caaaataaat gcatagactc ttttattttc gtctagatac
    14161 tttttgaata gctataaata aacctgcagc taaactgact actagacctg ataagaccca
    14221 agggaatcct ctttttgaag taggttttac ttcctctttg tccactagca tttttgtctt
    14281 gttgctaccg tttttttggc tattactagc agttggtgta agtttctcat ttttagtaga
    14341 tcgctttaag gaagttgatc gatgattagt agctgatttg aaaggatttg aagtagaatc
    14401 tatttcttgg ttagctgatt gatttgtttg agcttttgtg tctacatcga ccgaattcgt
    14461 cattgcttta gaagtatcct tttgttgcat attagttggt gtttttctag ctttgttttg
    14521 atcaaaatga acagttcctg attgaccagt tactgccttt tcttgagtat gaacggtgac
    14581 agttacatga tagtcaccac tcttttgtcc agttaaatca aaccaagctg taaaacgacg
    14641 ctgttgcgct gccatagtat atacccaact attaatagta ttatcactag cgtctttaag
    14701 tgttatcgtt gcctgagttg gttgtctgac aggttgatct gtggtgactg tagcatcaat
    14761 ataaggttct gttccacgca tactggcttc aacactgacc tgactaatag ttgattcagc
    14821 tataacatta tcagtgtgct gatcttgtgc atacacagat atagctttgc cagaagtccc
    14881 cagcaaacca ataagtaaag ctagtgataa taagctaact gttttttgca tagttgtcat
    14941 aataaatatc tcctctttaa attagtctta ccctctcatt ataaactatt cgaaaatgat
    15001 ttcataattt ttatgtcttt tttgttacaa atgtatcttt tttatatcat tatgtctttt
    15061 tgatgtcata aaaagactta atggctctct acctttactt cagatctatg aaaataagag
    15121 tctcctaggg gactcttatg aggatatcaa ttgttgcttt tctacatact caatacacca
    15181 tctgatcaat tcttctaacc gctctttttg gcccatcata tccagataac ccataatggc
    15241 ttcaaaacct gtcgacatac gataggtaat aatatcggca ttcttagctt tagtatggct
    15301 attggtattg cgaccacgct tatagatgtc ttcttctttt tcggtcaata gctgcgcttc
    15361 taacatagcc tgaatcaagt tggcttgtgc cttagcagaa acatacctcg ttgctaaacg
    15421 gtgtagctgg ctaggtttcg ttttaccttg aaaaatgaga tgacgacgaa catagcagga
    15481 ataaaccgca tccccttcaa aggctagggc aataccatta atcaaattca catcaactgg
    15541 attagtcacg cagccacctc acaccatctt tggtatctag cagcttgatg ccttgagctg
    15601 ctaactgatc tcgaatggca tcagctgtgg caaaatcacg attcgcgcgt gcttcttgtc
    15661 gcttagcaat caaggcttca atgtcaacct caagcacctc ttcttcaaag ataatgccaa
    15721 aaacagctaa cattttctca aaagcgcttt taacaggttc tgtataggag ccagaattaa
    15781 tccacttggc catgtcaaat acgacggtaa tcccatttgc tgtattgaaa tcatcgtcca
    15841 tagcgtcttg gaaagctatt acaaactgtt ttagctcttg ctcatctgca gtttctgtca
    15901 gcggttgttg caaggtgttt ttaagatact tcagattgat ttccgcatca tgaatagcct
    15961 tttcagtgaa gttaattggt tttcggtact gttgtgtcgc aagaaagaat cgcaaaacct
    16021 gaccatcaac ggtttgtaac atgtcatgaa cggtcacaaa attacctagt gacttggaca
    16081 ttttttcgtt atcaacagtg acaaacccat tgtgcatcca atagttagca aaagtcttgc
    16141 ctgttttggc ctcagattgg gcaatttcat tggtatgatg aggaaattcc aaatcagctc
    16201 cgccaccatg aatatcaatg gtatcaccaa gaatttcagt agccatcaca gaacattcaa
    16261 tgtgccagcc tggacgacca aacccccagg ggctatccca agaaacctca cctgccttag
    16321 ctgatttcca aagagcaaag tccagtggat tttcttttaa agctgtctca gcatctgttc
    16381 gaccactggc tccaacttca agttctgaca gggttttatt agctagcttg gcataatgct
    16441 ctgacttttc cacgcggaaa tacacatctc catctgcttc gtaagcaaaa tctttttcaa
    16501 tgagactttc aacaaatgaa atgatttctg ctatataatc cataacacga ggattttgtg
    16561 tggctggctt aacaccaagt gccttggtat cttctataaa agctgcaata aagcgatctg
    16621 acaattcttt gggagaaaca cctgcttgag tagcagcctt gataatttta tcatcgacat
    16681 cggtaaaatt ggaaatgtaa ttgacctggt agccagtata ctcaaaataa cgccgaatgg
    16741 tgtcaaaagc gaccgctgat ctagcatttc caatatgaat ataattatag accgtcggtc
    16801 cacaaacgta catattgact gtattttcag tcaaaggtac aaacttgtgg agcgaacggg
    16861 tcatggtatc ataaatttta atcatgtaaa ctccttattc aatagcctgg cattagttgt
    16921 catcttgata tcctaagtct ttttctgaaa tgctgttcca ccttttcaac aatttgatat
    16981 taataaccaa acctattaac caaaataggg tcaaaatgaa gtatataatc gctttaacca
    17041 tgtcttgttt cagtaaaaag attaaactag ccaccagtgt aatactagaa acaaggatag
    17101 acaaactcat aatgacatac agcccaactc ttattttatt catgaggctc ctttattaac
    17161 gaaaaacctt cacaacctgt gaaggcttca gaaagtagac aaacccactt ttatgctcaa
    17221 aaagcatgaa tagatctgct gatttgacat caggtttcct atttctgcat gtggggtaag
    17281 ccaaaacagc tgctttttgt tgtggtaaaa gtggcatcaa tactgacagt gatgcccata
    17341 cggatttttg agatcttagg ctcaaaacta aggcatggaa ttccaaagga agtcgctgcc
    17401 gtaagcacca cctgagaaaa agtatcacaa aagtcccaaa atattttatt ggaagtcatc
    17461 agtgcttctt tttgttattt tccgatcatt ttgacaactg ataagagacc tctcgttgtt
    17521 tttgtaaact ttgaatttga cgattatctt tttgcccatg cactcgtact atcttagctg
    17581 gcacacctac aactgtcacg tcttcaggaa catctgataa cacaacagct gctgccccta
    17641 ctttagcatt tgctccgatg tcaataggtc caatcacttg ggcatgtgcc gaaattaagg
    17701 caccttgtcg aatcgttgga tgacgcttgc cacaatcttt tccggttcca cctagggtca
    17761 ccccatgata aagcatcaca cctttttcaa caattgctgt ctctccaata acaagaccag
    17821 caccatggtc aatgaagact ccaggagcga tttgtgctcc aggatggatt tcaatttgtg
    17881 tccaaaatcg ccaaaattgg ctatgcattc tggctagtaa tttaaaatga tgtcgccaaa
    17941 gaaaatgaga caaacgatga gcagctaggg ctttgatgcc tggataagtt aggataacct
    18001 caagactatt acgagcagct gggtctagag ctttaacaat agctatactt tctttccacc
    18061 aacccatact aactcctacg acttatctaa ttctgaccaa tcaacaagaa tttgttggtc
    18121 atcaaaagga ttgatggcga caccgaacgt ttcatcaagc tcgttttcac catttcttgg
    18181 ttgataaata tcatcaatcg tccaagtcag aatcactcct tcagctttcc tgaaaaggcc
    18241 tccaaaatcg tcttggttgt accatttcgc aaagctttga agatttgaaa aggcgggaac
    18301 atagcttttt ccttcaggcg ttgacatggt aggaaaaagg cggtcgtaat ggttatcatc
    18361 tttaggataa acaaaggcgg gaacaaggta aaccttttcc atcgtatctg ccgccacatt
    18421 gtcatcactc ataagcgtat ttaacacagt ggtgtaatgg ttcataaatt gaatcatgtc
    18481 actgcttttg aaaatggtag aattcccaaa atctcccttt ttcttaaggt taaagacaag
    18541 acctgctgca ccagatgtaa tgacctcttc tagcactgca agagctgaac gctccagcca
    18601 atactgactt tgagcactct tttgttcttc tttaaagcga gccatatctt ccttatcggt
    18661 aaaaacaggg gtaaccttga caccttcgac ttctatcacg tagggttctt tagcagccca
    18721 gacaggaaaa ttatggaaag cgttgacaag ggcaaggcta tccaaaaagt tatcaggcgc
    18781 attgataaaa gctcttaagc gaatatctaa ttcatttgat ttagtcatgt ttttcttctt
    18841 ttttctctgg ttttggtggg cgtggaatca aagctttcat tgaggcatcc acacgaccct
    18901 tctcatcaat cttaatgacc ttaacatcaa cgtcttcacc cacttcaagg acatctgaca
    18961 cgttagctgt tctcgtccag gcaatttcag aaatatgaac aagtgcatct gtcttgtcaa
    19021 agaggttgac aaaggcacca aatttctcga tacggataac cttggcatgg taaacttcgc
    19081 ctaccttggc ttcacgaaca agacctgcaa taatttcttt agtgcggtca atggcagctt
    19141 ggtcacttga gtagatagag acatttcctt cgtcgtcaat atcaattttg acacccgttt
    19201 cggcaataat cttatcaata gtttcgccac ctttaccgat aaccactttg attttgtcta
    19261 cgtcaatctt gatggtgtca atttttggtg cagttggggc taactcagga cgtggttcag
    19321 cgatagttgc ttcaatcaca tcaagaattt caaagcgagc ttttttagct tgtgcaagag
    19381 cttcttctag gatttgaggg gtaattcctg caatcttaat atccatttga agggctgtaa
    19441 tcccttcacg agttcctgcc actttaaagt ccatatctcc aaagtggtct tcaagacctt
    19501 ggatatcggt caagactgta taattcgtcc cgtctgaaat gagacccatg gcaatccctg
    19561 ccacaggagc cttgattggt acaccaccag ccatcagagc aagggtacca gcacagatag
    19621 aagcctgaga agacgaaccg ttagattcta gaacttcggc aactaaacga atggcataag
    19681 gaaattcttc taagcttggc aagacttgtt caagagcgcg ttcacctaag gcaccgtgtc
    19741 caatttcacg acgaccagca gctccgtaac gacccgtttc accaactgaa tattgcggga
    19801 aattgtagtg gtgtaaaaag cgttttttgt actctggagc cagaccatca atgatttgag
    19861 tttctcccat tggtgccaag gtcaaaactg atagcgcctg agtttgacca cgtgtaaaga
    19921 gacctgatcc atgtactttt ggcaagaagt caaccacagc atcaagcgga cgaatttcgt
    19981 caatcttacg accatcagga cgaatcttgt cctctgtaat gaggcggcgc acttcagcat
    20041 gctccatttg ttcaaggatt tctgccacgt cacgcataat ggttgctagg ttttcgtctt
    20101 ctgcgtaacg ctcttcgtac tcagccttaa ccatttcttt aacggcttct gtggctgctt
    20161 cacgcgcttt tttctcttca acttgaacag ctttttgcag ttgagcattg tacttagcca
    20221 caatatcagc ttgtaagtct gcatcaacct gtaacagttc aacctcagct ttttctttac
    20281 caacaactgc tacgatttgt tcttggaagg caattaattc ttgaatagct tggtgacctt
    20341 ttaaaagagc ctcaagcatg atgtcttctg ataattcttt ggcaccagat tcaaccatgt
    20401 tgatggcttc tttgctacca gcaacggtta attctaagag cgacgcttcc atttgttctt
    20461 tatctgggtt gatgatgaac tcgccatcaa tatagccaac ttgaacacct gcaatcggtc
    20521 cattaaatgg aatatctgag atggacaagg ccagagatga gccaaacatc gctgccactg
    20581 gagcgctagc attttcatca taagaaagga cagtgttaat cacttgtact tcgttacgga
    20641 aaccttcggc aaacatggga cgaatcgggc ggtcaatcaa gcgagctgtc aaggtcgcat
    20701 ctgttgaagg gcgtccctca cgtttcatga aacctcctgg gaatttacca gcggcataca
    20761 ttttttcttc gtaattaact tgaagaggga agaaatcacc cgttgccatt ttcttagaca
    20821 tgacagctgc ggtcaggaca gttgagtcgc cataacgaac aacggttgcc ccattggctt
    20881 gcttagcgac ttgaccaact tcaacaacaa ggggtttccc tgcaaatgtt gttgtaaagg
    20941 tttgttttga catatattct ccttttaatt ttcagagggg taaagaggta cctgttctct
    21001 gcttagattc aattgttata cgttgttttg ctacaaaaag caacctgcca cgcttgtcac
    21061 acgctcctct ttgtatcaaa cgacgtatct gctacatttt accataaaat agcagaaatt
    21121 tcactgtcag atcgcaatct cttgaaatgt ccttgaaaaa tattaaacga aacttatact
    21181 ctttaacaaa gcaaaaaaag caggttggta tacctgcttt cgtctcctaa tattaatgtt
    21241 tggcatcctc taaaatttta aagccgaggc tggtactgtc tttcagaaac atttgcacgg
    21301 cttcaaggga atagtcttct aaatcttttc cttgaagagg caacttggca atcaaatcag
    21361 tggtacaagt gataatgtcc acgcctagct gatcagcttg atagatatta taagtttcac
    21421 gaggactagc ccacaacaat tcgacctctt ctttttgacg acaaatgcgt agagcttctt
    21481 ccatgattgg cattgggtca acgccggtat ctgcaatacg accagcaaaa acagacacaa
    21541 tagctggaac tccagcggtt aaatgatcaa ccacagcttg agtctgctca attgtaaaaa
    21601 tggctgtgac gtttaactta atgcctttgg ctgacaactt ttgaatgagg ggacaggttg
    21661 attctcctgt tgaagtggtg actggaattt tcacatagac attgtcacct agactggcaa
    21721 ttttctcagc ttccttttcc atgcttgcga gatcatccgc aaagacttca aaggacactg
    21781 ggtagtctgt aattttggca agcacttctt tggcaaagcc aatataagaa ctgatgcctg
    21841 cttttttcat caaacttgga ttggttgtaa agcctgtgac caaacctgtt tgtaaatcct
    21901 ttaacatggt ctctaagacc gcaccgtctg aaaagacttt aacgtttagt ttttttgtca
    21961 tcatatcctc cttttagatt tcttctgaaa cattattgat ggcttcttga gattttgaca
    22021 gaaagtttgg aagcaccaat acaatagcaa gcacagcaac caccaagtaa ataccgattt
    22081 gaccaatcga cgttcctaag cggcccagta aaatccctaa aacaccgaaa tccacatctc
    22141 caaaggtcgt gtttgaaaaa ccaagtttac caagtacagg tagaagcatg gctggcaaga
    22201 aggcgagtaa taagccatta gcgaaagcac caatcattgc accgcgacga ccacctgttg
    22261 aatttccaaa aatccctgct gttgcaccgc agaagaaatg aggcaccata cctgggataa
    22321 tcaggacacc acctgcaact cctaaaatca acatcccaag taagccacca acgaaacttg
    22381 acgcaaaacc gatgataaca gcggttggcg cataagggaa gaataccgca cagtctacag
    22441 ctggaatagc atttggaatt aatttgttag caataccttg gaaggctgga atcaagtcag
    22501 ccaaaatcat gcgaacaccg gcgtagacaa ttgccacacc aaccgcaaag gtaagaccac
    22561 tcttaatagc aaagataaat gggttttgac ctgctgctaa ttcttctgtt actgaagcat
    22621 tcctcaaaac agaagccact gttgccacca agtagaaaat caccatgata agtcctgttg
    22681 aaatggtggt gttgcgtaag aaactccatt tttcagaaat ctgaaggtcc tcggtatctt
    22741 tactgtcttt gccaaccttg ctaccaaacc aggcagaaag gtaatagccc aaactaccaa
    22801 agtgtcccat ggcgatttca tctccgtcag tcactttcaa cgtatactgt cgaccaatgg
    22861 ctggcgaaat agctgaccaa gctcccaaga ggaacccacc taagataatc aaaagacttc
    22921 ctttgaaacc aacagctcca agcacggcgg acaagagaca agccataaag aagctgtgat
    22981 gtcctgttaa gaaaatgtat ttgaagcggg taaaacgagc aaaagccaaa ttgagcaata
    23041 acccaaccac caagatggac atggtttcca cacccagaat cttttgagcg acagaagtga
    23101 cggcttcatt atttggcact acgccagtga tgctaaaacc gtgctcaatg agtttggcaa
    23161 gagggtccag attggccaca ataacacccg cccctgcagc aagcataagg tatcccaaaa
    23221 tcggccccaa ggttcctgtc aacaccttat gagcaggtgt tttcaaagcc accaaccctg
    23281 caaaggcaat caagcccatt aaaaatgcag gttcttttaa aatatctcga ataaatgata
    23341 ataatgcttc catagtatct tcctatctcc tttaaaggct tattccttgc cactcatttc
    23401 cttgagaacc ggaaccaaat cttcttcaat ttttttacga ttggtatagc ttttaacgat
    23461 acaaaccttg gcatcctctg gaaaaatatc tttaaattct ttaacggtaa caaacaaatc
    23521 tgcacccttt ccaacagccg cattggaatc acaggattct gcatccacat caatacccaa
    23581 actagatgcg atagcttcta ctttcatgcg aagtaacaag ctactaccaa taccgtttcc
    23641 acaaaccgtt acaattttaa tcatgtgatt catctccttt ttcaatcata tggctaatca
    23701 gactcattat ttcctcagta ttcttagcct caattaattg ctcaatatgt tcatcatcat
    23761 ctaaaatcaa cgacagttcc tgcaaagcct tcaaatgtga actagaatcc acagcagaca
    23821 aaacaaaaat caattgcact tgcttgtctg gatcaccttc ttgcttcaaa tcaaagctga
    23881 caggttcttt tagttgtaag agagacatgc ctaactgccg cgttcctttt tcaggcgccg
    23941 catgaggaac agccacctta ggggttaaaa caatataggc cccaagctca ttgactgaat
    24001 caatcatgcc atcgatataa gatgtttcaa tcatctggtg ttctagtaaa ggctgagcag
    24061 ccaaacgaat cgcttcctgc caatccgaaa cagcatcagc ctgcctaata aaatctgctg
    24121 ttaacaagtc caatagtcct agacctcctt tttcaatcgt taaatggtta ccaatcaggt
    24181 aacgggataa atcactaaca agtccttctt tattagtaat cgtggtgtgt ttatgaataa
    24241 tactcaacag gtcatccaaa gcgaattgct ctgacaaggg aagatgaaag tcatcacaga
    24301 ccatcttttt caacatcatt ttctcaacag gccccatcaa agcttgcgtc acataaacag
    24361 gtttagcaca gtcaaaagct actgttgaaa aaatcaaatc aaaggaagct ggatccaaca
    24421 actttatttt gtccagctgg tgaatcctaa taaactgtag ctgtggaaaa agttccttca
    24481 aggttgcctc caacatcaga gacgaactaa tgccattagg gcaaacagat aaggctacca
    24541 gctgattgct cggcctcttc ttaggggcct gcaaccatct cccaaaatga atggtaaaat
    24601 aagcaacctc atcttcatta actagtttcc ccaattgttt ttctagagga gaaaggctgc
    24661 gcttgaccaa gtaaaagaga gattcataat cttgtttgat ttgttccttc aaaggattgg
    24721 tcaagttaat gtcgtagtaa agtcggtaat aagctggcaa aaggtgactg tataggttct
    24781 gacgaagttc tggggtatcg gtgatagaca atcccgtgtt aaccgccacc gaattgatga
    24841 tttcttccat aatgtcataa ataggttgct gaaagactaa ctctaagtca ccttggatac
    24901 aaccaagtaa cctagattga accaaatatt tttcttcggt tagactgggg aagttgacca
    24961 ataactggtc caccatcgtt tcaacagctg gcgaagctaa aggaaaagta tccactgctt
    25021 cagcccttac attccgtgaa aatggtcgat gagcgagaag gcagaaaaaa taaatcaaat
    25081 ccctcaattt ctccgaaata aaagttaagt ggttctccct gctcagttcc tccaacgttg
    25141 ctgccatcac ctttgtctgg tcgggcaaac caagttcatg caataagtaa ctgaacaccc
    25201 aaggtcctga tgtaacttgc aaaagagaac tgaccgctga ttctaagaga cgccgcaagg
    25261 ctaagggttc tccttcaaaa aaataaccat cctggcgatt gtaagctaaa gttacctgaa
    25321 aagcttgtac ccgatcacgc accaagttta aatcagcaat aacggaattt ctgctgagcc
    25381 gtaacaagct ttccaagtgg ttaatggaga taaattcttt agccatcatg atgtaaatga
    25441 taatcatgtc cagacgctca tcttgaaaga gatagtcgtc taaggtaggt agcatgcgat
    25501 tcaacaagtc cttttcagta aggtctggcg gataaaccag ctgcctgtcc agagcaataa
    25561 ggggtaactg atagagtgac aacgtgtcat tgattttagc aatatctgcc agtatatgac
    25621 gtttgcttgt ctttaacatg gattcaaatg cctccagtga atgtccttta tatttagaaa
    25681 agagttgata gtttctgatg agctcatgag atagcatgtc atctcctcct ataatgtcag
    25741 tataaaatga agcgttttca ttttcaagac aaattgagtc caattgtctc tcgtcatttt
    25801 tgaccgcaat tggactcaac aggtgatatc attgtaacaa gttcctcaaa aggtggcaaa
    25861 aaatagacaa atctgggaat tgattccctt tttcttcatg atacaacaaa agatacctca
    25921 tcactgagat accttttgtt attagataaa atcatcaatt gctaaaaaaa caaccttaga
    25981 ccattctagc caaatgtgta agttaacaaa caacatgtca aaaatgaaaa agcccactgc
    26041 ataagcagag gactttttaa acttgaaatt aacgacgaag tccaagagat tggatcaact
    26101 cacggtaacg gttaacgtct gtacgacgta ggtatgccaa caagttacga cggtgaccga
    26161 ttttcttcat caatccacgg taagtagcgt ggtctttttt gtgttctttg atgtggttgt
    26221 tcaagtggtt gatttcccat gtaagaactg caacttgaac ttctactgaa ccagtatcac
    26281 cttcgtgacg tgcatattga gcaatgattt catttttttt ctcttttgag attgccataa
    26341 tatattctcc ttttttttgc ttaatccgag tcttaggatt ggcactccta aaaccaagaa
    26401 aaagttggtt tgtctttcga ccactctatt ctagcagatt cataatatta ggtcaaccgt
    26461 tatcctatta ttctaagctc tcacatccaa accaccttaa gcaacagaac tagcaaggcc
    26521 aaatagccaa gaggctgttt ttaaatcaga ataaacctgt gcgattgctt gcctaataag
    26581 ataaccagct aaaagaggta agatggctga tccccgcact tcagctgata agcggtaacc
    26641 tagcatagtc attcatttct ttccaacaaa gtgttcactg ataatattaa ttgctctagt
    26701 attaacaaac ccaatcccta atccatgaac acagcttatt aaccgatgca acctgccttt
    26761 atgacatagg tcaagagttg acacaaaatg tttgccaaca aaacattacc tataccgtca
    26821 aagagcgctt tgcttcctat atccttgaag cgcaagccaa ccaagaggtt catctgaact
    26881 ttacattatt agcaaaccgt tttggaataa gtgatcgcca tcttaagcac gtacttaagc
    26941 agctcatttt tcagaggatt atcgaacgca tctgcttcaa aacctgtcgt attgccgatt
    27001 tagacgctct aactcaatta agtgttaata actagacctc tgaggtatgc agcccattaa
    27061 cttataatag caatcactct gaccactacc tgacttttct ttaaaaaaga aatgatttgt
    27121 gatatagtaa atccagttaa aaaaaaagta aaggagtaga catgtctgca caagataaat
    27181 taattaaacc tagtcatctg attaccatgg atgacattat tcgtgaaggg aatccaactt
    27241 taagagctgt cgccaaagaa gtgagcttac ccttgtgtga tgaggatatc ctattaggag
    27301 aaaagatgat gcaatttttg aagcattctc aagatcctgt aatggcggaa aaacttggtc
    27361 ttagggccgg tgttggtcta gcagctcctc agattgatgt gtcaaaacgt atcatcgctg
    27421 tgctcgttcc taatcttcct gataaagaag gaaatcctcc taaagaagcc tatagctggc
    27481 aagaagtcct gtataatcct aaaatcgttt ctcattctgt tcaagatgca gccctttctg
    27541 atggtgaagg ttgtttatct gtcgatcgtg ttgttgaagg gtatgtggtt cgccatgctc
    27601 gtgttacagt agactactat gataaagaag gacaacagca tcgcatcaaa ttaaagggat
    27661 acaacgctat tgtggttcaa catgaaattg accatatcaa tggtgttttg ttctacgatc
    27721 ggatcaatgc taaaaacccc tttgaaacca aagaagagct cttgatttta gactaacaaa
    27781 aaaaggccct ttagcctttt tttgttagtc taaatagccg ctaaaagagc ctctgcctga
    27841 gatagcaagt ttgcttttgt ctcttttgaa atctcaagcc ttcctgttcc ccaggcatca
    27901 ggattcacag ttgcttttgt aaactctcct gctactgaag ttcggataaa cggcaatagt
    27961 gctttaaact gatcaaatac ttgatcatgc ccgccatttg caactgaaga gaccgtaacc
    28021 accttaccgc caatagcaga tgggcccgtc ggatcagaca aatcaagagc acgagacaac
    28081 cagtctagca ggtttttaac agaacctgga atagagaagt tgtaaactgg tgtaaaaatc
    28141 cagatagcat ccgctgactg aacagcttga cgagcgtcaa caactggtaa aggtgcatta
    28201 gcttcgatat cttgattcaa aacaggaacg tctttccaat ttaagtaagc tacaactgct
    28261 tgatgttcca gagctttttg tgcttgagcc gctaattgat ggttaaaaga cccttcacga
    28321 agcgagccaa caataaataa aatatgtttc atctctttgt cctctttttt aaagatcgtt
    28381 ttaaagcttt ctaaaagtgg tat
//
LOCUS       DAUELZ010000009        28157 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.009, whole genome shotgun sequence.
ACCESSION   DAUELZ010000009 DAUELZ010000000
VERSION     DAUELZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28157
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.009"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            332..568
                     /locus_tag="VHI09_000320"
     CDS             332..568
                     /locus_tag="VHI09_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEQ6120226.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGAGEGFSMWAPTPCDVLAEDWIEVND"
     gene            561..1259
                     /locus_tag="VHI09_000321"
     CDS             561..1259
                     /locus_tag="VHI09_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEQ6120227.1"
                     /translation="MTKVVLVTGCASGIGYAQARYFLSQGYHVYGVDKSDKPDLNGNF
                     HFIKLDLSSELSPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRHMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLADWVARETPIGRWTEPSEVAELTGFLASGKTRSM
                     QGEIVKIDGGWTLK"
     gene            1335..2294
                     /locus_tag="VHI09_000322"
     CDS             1335..2294
                     /locus_tag="VHI09_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ6120228.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYKMPLATSKYEAILGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIAKKRKLILLEAITNQYLSNMTFIKEHLDQLGDIKIVECNYSQYSSRYDAF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKSTIQGNKGSLAVLGATNSLPQVQLSLHGHEPQVINHNKY
                     EHRMYEEFVAFKDMIDQKDFEKVNQALEHSRAVMAVLERAVHS"
     gene            2627..3964
                     /locus_tag="VHI09_000323"
     CDS             2627..3964
                     /locus_tag="VHI09_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ6120229.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISILEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            4087..5469
                     /gene="glmU"
                     /locus_tag="VHI09_000324"
     CDS             4087..5469
                     /gene="glmU"
                     /locus_tag="VHI09_000324"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEQ6120230.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIN
                     PEKAVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGDNCVVTNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDNALTAAGSTISKTVPADSIVIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            5500..6054
                     /locus_tag="VHI09_000325"
     CDS             5500..6054
                     /locus_tag="VHI09_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ6120231.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKIVLVKQYRKAIESVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            6054..6305
                     /locus_tag="VHI09_000326"
     CDS             6054..6305
                     /locus_tag="VHI09_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120232.1"
                     /translation="MGRPLLTDDIIEKARRMETFEPDDAVDFDTKVMTLPEKDEKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            6325..7020
                     /locus_tag="VHI09_000327"
     CDS             6325..7020
                     /locus_tag="VHI09_000327"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEQ6120233.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELV
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDV
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQNKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQILMTFLENLPV"
     gene            7171..7512
                     /locus_tag="VHI09_000328"
     CDS             7171..7512
                     /locus_tag="VHI09_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120234.1"
                     /translation="MTIKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGGYRQGLTD
                     EKSHLQYSKDNAQLQLRNILNGYQDDLGRNYSSYYYYNLRTVMGLSKEQKIEEHYEKL
                     KDELYRMYNHY"
     gene            complement(7609..8256)
                     /locus_tag="VHI09_000329"
     CDS             complement(7609..8256)
                     /locus_tag="VHI09_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEQ6120235.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLETHHLNINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
     gene            8402..9334
                     /locus_tag="VHI09_000330"
     CDS             8402..9334
                     /locus_tag="VHI09_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEQ6120236.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            9398..10123
                     /locus_tag="VHI09_000331"
     CDS             9398..10123
                     /locus_tag="VHI09_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120237.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIDGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            10124..10978
                     /locus_tag="VHI09_000332"
     CDS             10124..10978
                     /locus_tag="VHI09_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ6120238.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLGVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(11126..11932)
                     /locus_tag="VHI09_000333"
     CDS             complement(11126..11932)
                     /locus_tag="VHI09_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEQ6120239.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPEQDITITSIDIVKDYRFKN"
     gene            12149..14554
                     /locus_tag="VHI09_000334"
     CDS             12149..14554
                     /locus_tag="VHI09_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ6120240.1"
                     /translation="MVKRNQRRKSAPKKRLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQDIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHLVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMTKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVEVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKIGVRNIA
                     GYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRMAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(14624..14977)
                     /locus_tag="VHI09_000335"
     CDS             complement(14624..14977)
                     /locus_tag="VHI09_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEQ6120241.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTSLFKLNKKGFNFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            15221..15646
                     /gene="rplK"
                     /locus_tag="VHI09_000336"
     CDS             15221..15646
                     /gene="rplK"
                     /locus_tag="VHI09_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEQ6120242.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            15752..16441
                     /gene="rplA"
                     /locus_tag="VHI09_000337"
     CDS             15752..16441
                     /gene="rplA"
                     /locus_tag="VHI09_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEQ6120243.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            16763..17491
                     /locus_tag="VHI09_000338"
     CDS             16763..17491
                     /locus_tag="VHI09_000338"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEQ6120244.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            17520..18077
                     /gene="frr"
                     /locus_tag="VHI09_000339"
     CDS             17520..18077
                     /gene="frr"
                     /locus_tag="VHI09_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEQ6120245.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKATDDAIKEIDRMTAEKEKELLSV"
     gene            18186..19043
                     /locus_tag="VHI09_000340"
     CDS             18186..19043
                     /locus_tag="VHI09_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ6120246.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQVGDM
                     VTGFAYIDIKQKARLTTKEIRSTRTSYGWGEVTEIRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYIRLDVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKAIFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            19116..19625
                     /gene="msrA"
                     /locus_tag="VHI09_000341"
     CDS             19116..19625
                     /gene="msrA"
                     /locus_tag="VHI09_000341"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEQ6120247.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPEEISYKELVELYWAQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPKRYAQSSAIR
                     HQFLEENWS"
     gene            19622..19837
                     /locus_tag="VHI09_000342"
     CDS             19622..19837
                     /locus_tag="VHI09_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEQ6120248.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            19993..21174
                     /locus_tag="VHI09_000343"
     CDS             19993..21174
                     /locus_tag="VHI09_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ6120249.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTEIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQASATSQSTVPMAPSATPSDVPTTPFASAKPDSSVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDSTSANRP
                     VPNESASEEVSSAAPAQAPAEKEETSQMLTAPAAQKAVADTTSVATSNGLSYAPNHAY
                     NPMNAGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPENSALGDQV
                     AQYAIDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFN
                     A"
     gene            21490..23262
                     /locus_tag="VHI09_000344"
     CDS             21490..23262
                     /locus_tag="VHI09_000344"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEQ6120250.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIRLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELIHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWIYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEAKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKDNETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            23421..24473
                     /locus_tag="VHI09_000345"
     CDS             23421..24473
                     /locus_tag="VHI09_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEQ6120251.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            24519..25094
                     /locus_tag="VHI09_000346"
     CDS             24519..25094
                     /locus_tag="VHI09_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEQ6120252.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            25253..25750
                     /gene="ybeY"
                     /locus_tag="VHI09_000347"
     CDS             25253..25750
                     /gene="ybeY"
                     /locus_tag="VHI09_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEQ6120253.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            25731..26138
                     /locus_tag="VHI09_000348"
     CDS             25731..26138
                     /locus_tag="VHI09_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEQ6120254.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            26258..27154
                     /gene="era"
                     /locus_tag="VHI09_000349"
     CDS             26258..27154
                     /gene="era"
                     /locus_tag="VHI09_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEQ6120255.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLAQIDDFRSQMEFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMIREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            27174..27650
                     /locus_tag="VHI09_000350"
     CDS             27174..27650
                     /locus_tag="VHI09_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEQ6120256.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTDDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     gene            complement(27955..28134)
                     /locus_tag="VHI09_000351"
     CDS             complement(27955..28134)
                     /locus_tag="VHI09_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120257.1"
                     /translation="MRYPHAHIILIGNHINYEEIFKNHYRVFGVIDTTSHKSLRSIRD
                     QIQLYLDELYNSNNG"
BASE COUNT         8627 a         4983 c         6196 g         8351 t
ORIGIN      
        1 ctaaaaaatg ttatacttaa tgaagaaaaa ggagagttta gaacgttaag atacctcaca
       61 gcagattgat agaggatatg gaagaaggtt agctttccca agagatgccg tttgggaatg
      121 gtataacaat aactgggaga agttataagt aaaacaagaa caaattaact cagatgattt
      181 ttagacgttt ccacgcgcgt gcgaattgat gttatcaata aatcctctaa taacgccttt
      241 ttcgaacagg aaacaaattt attatcccac gcgtgtgcga attaattgga gaaaaaatga
      301 gaaaatacga tatacaaaaa aggagaacaa tatgacattt gaagaaattc taccaggttt
      361 aaaagccaaa aaaaaatatg tacgtacagg ttggggtggc gctgaaaatt atgtgcagtt
      421 gtttgatacc cttgaagtgg atgggaaagt actacaggcg acaccttatt ttctgattca
      481 tgttactggt gcaggtgaag gtttttccat gtgggcaccg acaccatgtg atgtgttagc
      541 agaagattgg atcgaggtca atgactaaag ttgttttagt gacgggctgt gctagtggga
      601 ttggttatgc acaggcccgc tattttttga gccaagggta tcacgtatat ggtgtggata
      661 aatcggataa accggactta aacggtaatt ttcactttat caaattggat ttatcctctg
      721 agttatcccc actatttaaa gtggtcccca gtgttgatat tttgtgtaat actgcgggga
      781 ttttagatgc ctacaaacca cttttagacg tcagtgatga agaagtggaa cacctatttg
      841 atattaattt ttttgcgacg gtgaagttaa cacgtcatta tcttagacat atggtggaaa
      901 agcagtcagg tgttatcatc aatatgtgct caatagcaag ttttatagca ggtggcggtg
      961 gtgttgccta tacgagttct aagcatgcgc ttgcgggctt cacgcgccag ctggctttgg
     1021 attacgccaa agatcagatt cacatctttg gtatagcacc aggtgctgtc aaaaccgcta
     1081 tgacggctag cgactttgag cctggtggct tagcagactg ggtagctcgt gagacgccaa
     1141 ttgggcgttg gactgaacca agtgaagttg ctgaattaac gggatttctt gcttcaggaa
     1201 aaacgagatc aatgcaaggt gaaattgtca aaattgacgg tggttggact ttgaaatgat
     1261 cacatgcctt agcttgagct ggcgcagtaa gccctagagg tgtgatgagt caaacagtca
     1321 aaggagatga acaaatgaaa ttagcggttt tggggacagg tatgattgtt aaggaagttt
     1381 tgcctgtctt gcagaaaatt gatggtattg atttagttgc tatcttatca acagttagaa
     1441 gtttaaagac agctaaagac cttgctaagg catataagat gcctctagct acgagtaaat
     1501 atgaggctat cttaggcaat gaggagattg acacagttta tattggattg ccaaatcact
     1561 tgcattttgc ctatgctaag gaggctcttc tggctggtaa acatgtcatt tgtgaaaagc
     1621 cctttaccat gacagcagga gaattagatg aactggtggt gatcgctaaa aaacgaaaac
     1681 tcattttgtt agaagcgatt accaatcagt atttaagcaa catgaccttt atcaaagagc
     1741 atcttgacca acttggcgat atcaaaattg tagaatgtaa ctattcacaa tattcttcac
     1801 gctacgatgc cttcaaacga ggtgatatcg cgccagcttt taatcctaaa atgggtggtg
     1861 gagctttacg tgatttaaac atttacaaca ttcattttgt ggtagggtta tttggtcgcc
     1921 ctaaaacggt tcaatattta gccaatgtcg aaaaaggcat tgacacatca ggcatgcttg
     1981 tcatggacta tgagcagttt aaagtggtat gtatcggggc caaagactgc actgcagaaa
     2041 tcaaatcaac catccaaggt aataaagggt cactggcagt gcttggagca acaaatagcc
     2101 ttcctcaggt tcagttaagt ctacacggtc acgaaccaca agtgattaac cacaacaagt
     2161 atgagcaccg gatgtatgag gagtttgtcg cttttaaaga catgattgac caaaaagact
     2221 ttgagaaagt gaatcaggct ttggagcata gccgagctgt catggctgtg ttggagcgag
     2281 ctgttcattc gtgatggtaa tctggtagcc attgcctttt gtgcaggtgc tatggtaatg
     2341 gttgaaagac gtgttttcac gagggttaac cccttcgtgc ttttttggca aaaataactt
     2401 gttcaggatt tgggctttct ttgtgagatg agagatgcct gacgaattga tttgattaaa
     2461 ttaatgataa agccaaacga tttaagaaat gaccgttcgg aaattctgcc ccaaagggtc
     2521 tctagaagca gtgaaatcga atttgtaagc gttgacactg ctacataact ctgttacact
     2581 aagagaatgt catgacaaaa attacagtgt aggagtttaa ttgtttatgg aagaggatta
     2641 taacaaaaga gaacctgaga agtttacgca atttctgcgc cgtcaaaagg tcgtcttttt
     2701 tgtcgcattt tttggttatg tgtgtgccta cttggtacgc aacaatttca aactaatgtc
     2761 aaacactatt atggtccaaa acggttggga caaggctcaa attgcgacct tgttatcgtg
     2821 tttgaccgtt tcttatggtt tagcaaaatt ctatatgggg gccttagggg atcgtgttag
     2881 cttgaggaag ctcttttcaa tcagcttggg tgcaagcgct ttgatttgta ttttaatcgg
     2941 ttttttcaat agttcaatgg tggtgttagg gatactgcta gtcttgtgtg gtgtcgtaca
     3001 aggtgccttg gcacctgcct cacaagccat gattgccaat tattttccaa ataaaacacg
     3061 cggcggagcc atcgctggat ggaacatttc tcaaaacatg ggatcagccc tcttgccatt
     3121 aaccatcgcc ttgctcacca gtatgggctt ggtggtacca gctaatggca atattttact
     3181 tgccttttta atccctggtg ttttagtgtt cttgtttgct ttgtgttgct ggaagcttgg
     3241 cggtgataac cctgagtctg agggacttga ctctcttcga acgatgtatg gtgatgcggg
     3301 cgaatcagct gttgccagtg aagaagaaaa gcataacctg tcttattggc aactcatctg
     3361 gaaatacgtt ttttgtaacc cgtcactctt acttgttgct gctgtcaatg tggcccttta
     3421 ttttgttcgt tttgggattg aagactggat gccgatctac ttgtcacaag tagccaatat
     3481 gtcagaggcc catattcatt ttgcgatttc aatattagag tgggtcgcta ttccaggctc
     3541 gctggtattt gcgtggttag cggttcgtta tcctaataaa atggccaaga ttggggctat
     3601 tgggcttttt gtgttagcgg ctattgtgtt tgtctatgaa cgcttgactg ccacaggtgc
     3661 tccaaactat ttcttgttgc ttgttattgc aggtatttta gggtcattga tttatggccc
     3721 acagttgatc gtgaatattt taacaatcaa ctttgttcct ttaaatgttg caggaacagc
     3781 gattggtttt gtaggagtaa cagcttatct catcggcaat atgggagcaa actggctgat
     3841 gccgattttg gcagatggtt ttggctggtt ttggtcatat attgtcgttg cagccttatc
     3901 tgctttttca gcggttggtt atttgatttt agccaaacgt gaggaagaaa tcatcaaaga
     3961 ttagctaggc atcacaagcc gcaagtgaca ctataaaaga caagaaaatg ctaggatgtt
     4021 tcaggaaaaa aagagaaaat atgctataat gagaaggtat ttaaattata tagtaaggaa
     4081 gaacaaatga caaattatgc cattatttta gcagcgggca aagggactcg catgacttct
     4141 gatctcccaa aagttcttca taaagtatct ggactaacca tgcttgagca tgtttttcgt
     4201 agtgtaaaag cgattaatcc tgaaaaagcc gtgacggtta ttggtcacaa atcagaaatg
     4261 gtacgtgctg tcttagcaga tcaatcagcc tttgtccatc aaacagagca gttaggaact
     4321 ggccatgccg tgatgatggc agaaacacaa ctagaaggac tagaagggca taccttggtg
     4381 attgcaggag atactccctt gatcactgga gaaagcctga agagtctgat tgattttcat
     4441 gtgaatcata aaaatgtcgc cactatttta acagctacgg ctcaagatcc atttggttat
     4501 ggtcgtattg ttcgcaacaa agatggcgaa gtgatcaaga ttgttgagca aaaagacgcc
     4561 aatgagtatg agcaacaatt aaaagaaatc aatacaggaa cctatgtgtt tgataacaag
     4621 cgtctctttg aagcgctcaa atgcatcact accaacaatg ctcaaggaga gtattacctg
     4681 actgatgttg tggctatttt tagagcaaac aaagaaaagg taggagccta tatcctgaga
     4741 gacttcaatg aaagtcttgg tgttaatgat cgtgtggcct tagcaacagc tgaaacagtg
     4801 atgcgtcagc gcatcaccca aaaacatatg gtcaatgggg tgactttcca aaatcctgaa
     4861 accgtttata ttgaaagcga tgttgagatt gctccagacg tccttatcga aggaaatgtg
     4921 accttgaaag ggcgtacaca tatcggctca ggaactgtct tgacgaatgg tacttatatt
     4981 gtggattctg aaattggtga taactgtgtt gttactaatt caatgatcga atcatctgtt
     5041 ttagctgcag gagttacggt tggcccttac gcccaccttc gaccaggaac gaccttagat
     5101 agggaagtcc atatcggtaa ctttgttgag gtcaagggtt ctcacattgg tgaaaaaaca
     5161 aaggctgggc atctgactta tattggaaac gctcaggttg ggtcttcagt caatgttggg
     5221 gctggaacca tcactgtcaa ctacgacggc caaaacaaat atgaaacagt cattggagat
     5281 cacgctttta ttgggagcaa ctcgactctc attgcacctt tggaagttgg agataatgct
     5341 ttaactgcag ctggctcaac tatttctaag acagtaccag ctgatagcat tgttattggt
     5401 cgtagccgcc aagtcacaaa agaaggttat gccaagcgcc tagcgcatca cccaagtcga
     5461 agcaaataat tcaagatcta tttagtaagg aggccaggca tgaaatttga agaaaaaacg
     5521 cttaagagac aaacagtttt tgatggtcat atttttaaag ttgttgtgga tgatgttgaa
     5581 cttcccaaca atctggggca atccaagcga gaattaattt ttcatcgtgg tgccgttgct
     5641 gttttagcta ttactcctga acgtaagatt gtccttgtca aacaataccg taaggctatt
     5701 gaaagtgtct catacgagat cccagcgggt aagctagaga ttggtgagga aggctcaaag
     5761 ctaaaagctg ctgcacgtga gctagaagaa gaaacagcct atacggggac attgacgttt
     5821 ttgtatgaat tttatacagc gattggtttt tgcaacgaaa aaatcacact tttcttagcg
     5881 acagacctaa cacaagttgc caatcccaaa ccacaagacg acgatgaagt tatcgaggtc
     5941 ttagagttga catatcaaga gtgcatggac ttagtcgctc aaggtaaact tgctgacgct
     6001 aaaacgttga ttgctttgca atactatgcc ttacattttg gaggtgagca gtgatgggaa
     6061 ggccattgtt gaccgatgat atcatcgaaa aagcgagacg aatggaaaca tttgagccag
     6121 atgatgctgt agactttgac acaaaagtaa tgacgctacc agaaaaagat gaaaaagcac
     6181 gcatttataa aagccgtcgg attgaaaatg ccaaacgcag ccaattgcaa tccaaattaa
     6241 atgtgatttt gatagctgtt atgctgttaa ttgccatctt ggtatatgct atcttttatc
     6301 tttaaaaaaa cgtaaggaaa tgatatgaaa ataggaatta ttgctgctat ggaggaggag
     6361 ttgtccctgc tcttagcaaa cctcttggat gctcaagaac atcaggtgct ttcaaaaact
     6421 tactatacgg gtcgttttgg caagcatgag ctcgttctgg tacagtcagg tgttggaaaa
     6481 gtgatgtctg ctatgacagt tgcgatcttg gttgagcatt tcaaagccca agctattatc
     6541 aatacaggat cagcaggggc agtcgcttca catttagcga tcggagatgt ggtggttgca
     6601 gatcggttag tgtatcatga tgtggatgtt actgcatttg gttatgctta tggtcaaatg
     6661 gctggtcaac cattatacta tgattgtgat ccacagtttg tggctatctt caaacaagtc
     6721 ctaaaacacg aaaagacaaa tggccaagtt ggtttgattg caacaggaga cagttttgtt
     6781 gctggtcaga acaagattga ccagatcaaa acagctttta gcgacgtgtt agcagtagaa
     6841 atggaaggtg cagctattgc tcaagcagca catactgcag ggaaaccttt catcgttgtt
     6901 cgtgcgatga gtgataccgc agcgcatgat gccaatatta cttttgatca atttattata
     6961 gaagctggca agcgttcggc ccagatatta atgacatttt tagaaaactt acctgtttaa
     7021 ctcaccttat cgtgagacaa ttagctggat aatctctatt ttaggagtct ggtcacctct
     7081 tgttttgaaa cctaaaaata agaattgcaa tcacttgacc aaacgctata atgggttaag
     7141 gaacgataga ttactaaaag gagtctaaaa atgacaataa aaaaacacta ttatcttctc
     7201 agtctgctag ctcttgtaac ggttggtgct gcctttaaca caagccagag tgtcagtgca
     7261 caagtttata gcaatggagg atatcgtcaa ggtttgactg atgaaaaatc acacctgcaa
     7321 tatagtaaag acaacgcaca acttcaattg agaaatatcc ttaacggcta ccaagatgac
     7381 ctagggagaa actactctag ctattattac tacaacctaa gaaccgttat gggactatca
     7441 aaagagcaaa agattgaaga acactatgaa aagcttaaag atgagctata ccgcatgtac
     7501 aatcattatt aagtcaaagg cttaaaaatg gcgactagcc cctcaaaaag aggccacact
     7561 catttaaatt taaatgagtg tggcctcttt ttgaggagac gcaaggggtt aaagggctgt
     7621 gacatagagc tgcttagcaa tattttcagg gatgacaagt tccttgtcgc cataacaaat
     7681 cgtataggtt tttgcaaagg tatcaatttg agttaaggtt agctcggtat tgatattgag
     7741 gtgatgtgtt tctagatact ggattaaatc aaagtggtcg tggattctgc ttaggcgaaa
     7801 ccgtcccaac tccgtgatgg tgttgagagt tgtagtgttc atttcgacaa gaggctgacc
     7861 atagcgagga atagtccctc cgtgtgggca gaaatctgga aaatcaagga tcttgtctaa
     7921 acgatcgata aaggtgtctg agacagtgtg ctctaagact tcagcttctt ggtgcacttc
     7981 ttgggtatta tagcccagct gatgaattaa aaatacctca atcaagcggt gtttgcggta
     8041 gaggttagca acaagagcat agcctttgtc ttttagcaaa tagccttttg ctttatcttt
     8101 gacaatccag ccttgtgaaa tcattttttt aatcatctct gaaacggcag gtgccgaaac
     8161 gtgcatcttt tctgcaacca ttttatttgt aatcttgggt tcttgttctc caatttcata
     8221 gatacatttc aagtaatctt ctttattagg cgtcatatcg tttggccttt ctcttattta
     8281 taatgattat tatatcagaa tttttaggaa aaagcttgac aaaaagccta aaagaggata
     8341 tccttatagc ataattaact aaacttaatt aaataaataa tatatcctaa ggagagaaat
     8401 gatgggaaag agaatgagcc tcattttggg tgctttttta tctgtttttc ttttagtagc
     8461 ttgttcgtca actggcacta aaactgctaa gagtgataaa ttaaaagtcg tggcaaccaa
     8521 ttcaattatt gccgacatga caaaagctat tgctggtgat aaaatcgatc tgcacagcat
     8581 tgtgccaatc ggtcaagacc ctcatgagta cgaaccatta ccagaagatg ttgaaaaaac
     8641 aagtaatgct gatgtgattt tctataatgg tatcaatcta gaagatggtg ggcaagcttg
     8701 gttcactaaa ctagtgaaaa atgctcaaaa aacgaaaaac aaagattact ttgccgtgtc
     8761 tgatggcatt gatgtgattt acttggaagg tgcaagcgaa aaaggaaaag aagatccaca
     8821 tgcttggtta aatctcgaaa acggaatcat ttattcaaaa aacattgcca aacaattgat
     8881 tgcaaaggat cctaaaaaca aagaaactta tgaaaagaac ctaaaagctt atgtggctaa
     8941 attggaaaaa ctagacaaag aagctaaatc aaaatttgat gctattgcag aaaataaaaa
     9001 attgattgtg actagtgaag gctgcttcaa gtacttttca aaagcttacg gtgtcccatc
     9061 tgcttatatc tgggaaatta acaccgaaga agaaggaaca ccagatcaaa tttcatcatt
     9121 gattgaaaaa ctaaaagtca tcaagccatc tgcgcttttt gtagagtcaa gtgtcgatag
     9181 acgccctatg gaaactgtct ctaaagatag tggtattcct atttattctg agatctttac
     9241 agattcaatt gctaaaaaag gtaaacctgg cgatagttat tatgctatga tgaaatggaa
     9301 ccttgacaaa atttctgaag gtctagcaaa ataagataac aataaagcac agcgatttta
     9361 gtgttgtgct ttgttttatt tatgtaaagg agacaaaatg attacaacta acaatctttg
     9421 tgtgacctac gatggcaaca gtaatgcttt agaagcgatt aatgtgacaa ttgatggtcc
     9481 ttctatcgtt ggtattatcg gtcctaatgg agctggaaaa tcaaccttta tgaaagctat
     9541 cttaaatctt attgattatc aaggtcacgt aacggttgat ggcaaagatg gtcgtaagct
     9601 tgggcataca gtagcttacg tagaacaacg aagcatgatt gactataatt ttcctattac
     9661 tgttaaagaa tgtgtggcgc tagggactta tagtaaatta ggtctttttc gccgagtagg
     9721 aaaaaaacaa tttgagcagg ttgataaggt tctcaaacaa gttggtttag aagactttgg
     9781 ccatcgaccg ataaaatcct tatctggtgg tcagtttcag cggatgttgg ttgcgcgttg
     9841 cttgattcaa gaatcagact atattttctt agatgaaccg tttgttggca ttgactcggt
     9901 cagcgaaaaa atcattgtag atcttttgaa agagctcaaa atggctggca aaacgatttt
     9961 aattgtacat catgatttga gcaaagtaga gcattatttt gacaaactca tgattttaaa
    10021 taaacatttg gtggcttatg gcaacgtttg tgaggtattt actgttgata ccttgtcaaa
    10081 agcttatggc aatcatttga ttttaggaaa ggagatggtg taaatgtcta tgaaattttt
    10141 tgagggcttg atgtcatacc actttttaca aaatgcactg ataacggctg tagtcattgg
    10201 tatcgtctca ggtgctgtag ggtgttttat tatccttagg tcaatgtctc ttatgggtga
    10261 tgccatctca cacgctgttt taccaggggt tgctttgtca tttattttag gagtcaattt
    10321 ttttattgga gcgattattt ttgggttatt agcttctgtt attattactt atatcaagga
    10381 aaactctgtc attaaaggag atacggctat tggtatcacc tttagctctt ttttggcact
    10441 gggagtcatc ttgatagggg tagccaatag ttcgacggac ttatttcata ttttgtttgg
    10501 gaatatttta gctgtgcaag acagcgataa gtggattact attggtgttt cgatttttgt
    10561 tttaggggtt attagtctct ttttcaaaga actattatta acatcatttg atcctatctt
    10621 agccaaatca atgggtgtga aggtcaatgc gtatcattat ttgttgatgg tcctattaac
    10681 tttagtggct gttacagcga tgcaaagcgt gggtactatc ttgattgttg ccttattgat
    10741 tacgccagca gcgacagctt atttatatgc taatagctta aaagtaatgt tagtgatgtc
    10801 atctttacta ggcgctttag catcggtttt agggctttat ttgggctata cctttaatgt
    10861 tgccgcaggg tcaagtatcg tactgacttc tgcgatgatg tttttgatca gtttctttgt
    10921 ttcacccaag caaggctacc tcaaaagatg gatgcaaaaa aaagaaaaaa caccttaaac
    10981 ttttcgcttt aaacacctta gtggtttgca aatagtagca aataacatca aagggtgttt
    11041 tttatatgtc atcaaaagac cataaaaaac cttatcaaac caaggtattt agcctattat
    11101 ctgataaggt ttttttaagc atgatttagt ttttaaagcg ataatctttg acaatgtcaa
    11161 ttgaggtaat cgtaatgtct tgttctggtt tatcattttg gttgatagaa gtcgcggcaa
    11221 ttttatcgac aacatccata ccatcaatga cttgaccaaa tacagtataa ccgccatcta
    11281 agcttggatt gccgccatgc tcataggcag agatgatagg ttttgggtag ttggtacttg
    11341 ataatccctt gctttgattt ttcttgtttt ggttgatata aaattggcta ccgttagtat
    11401 tagcaccagc atttgccatg gcaagagcac cgcgaatatg atataaaaat ggagagattt
    11461 cgttgacaaa gccattgcca gcatcttttt taggatcctt gcccttccaa atcgattcac
    11521 cacctgtgcc atctcctttg gggtcacctg attgaatcat aaagtcgttg atcacacgat
    11581 ggaaagtaag gttatcatag tagccttttt tagcatgggt gaggaagttc tcaacagcta
    11641 agggagcata ttttgggaat aacttaaggg taatgtcccc ttgacttgtc ctcattacga
    11701 ccttagcttc ttctttgccg acttctttgg ttagttgtgg aaattggcta gcatcagctt
    11761 tgagagcttt ttgaatgctt tcttcatagg ctttagaagc tgcttcgctt tcttctttgg
    11821 cagttttttc atcaatgtat ttatcgcctt ttatagcgcg gtcaacagat tcacaagcac
    11881 tcaaactaag taaactaatg gctactaaac tgagagataa taattttttc atgtctactc
    11941 ctaagaatga ttttctttat tttatcataa aatgcaacag aaaatagtag taaagagtta
    12001 aaaacgccta tttttgtgtt ttatgatcta accttggtta aaaggctgct acgactgtta
    12061 ttatagcaca agatgctata gaaaaactta aaataccttg gttttttaga gctatttcct
    12121 aatagttaaa aaatggtata atagtgttat ggttaaaaga aatcaaagaa gaaaaagtgc
    12181 gcctaagaag cgtctgacaa aagcagaagt ggaaaagcaa cgtgccatta agaggatgat
    12241 tttatctgtt ttaatggctt tgttgcttat ttttgcgatg cttcgcttgg gcgtctttgg
    12301 cgtgacaact tataatatga ttcgtttttt ggtgggtagt ttagcctatc cgtttatgtt
    12361 tgcctggtta atttacttat tttgttttaa atggttacgg caaaaagatg gaatgattgc
    12421 gggagttgtt attgcctttt tgggattgct agttgagtgg cacgccttct tatttgcgat
    12481 gcctaggatg cttgatcaag atatttttct aggcactgcg cggctaatta caagagattt
    12541 actagccctg cgcgtgactg aatttgttgg tggaggaatg cttggtgcat tactttataa
    12601 acccatagcc tttttatttt cgaatatcgg ctcttacttt attggttttt tgtttatctt
    12661 attaggatta tttctaatga cgccttggga tatctatgat gtgagtcacc ttgtgaaaga
    12721 ggctgttgat aaactagcag tggcttatca ggaaaataaa gaaaagcggt tcatcaaacg
    12781 agaagagcac cgcttacaag ctgaaaaaga ggctttagag aaacaagcac aagaggaaga
    12841 aaaacgtcta gcagaattga cggttgatcc tgagacggga gagattgttg aggatagtca
    12901 gtctcaagtc agctatgatt tggctgagga tatgacaaaa gagccagaga ttcttgctta
    12961 tgatagtcac ctcaaagacg atgaggcaag cttatttgac caagaagact tggcttatgc
    13021 tcatgaggaa attggggctt atgactcttt atctgcttta gcatcttctg aagatgagat
    13081 ggatatggat gaacctgttg aagttgattt cacaccaaaa acccatcttc tttataaact
    13141 tccaacgata gatttatttg caccagataa gcccaaaaac cagtccaaag agaaaaactt
    13201 agtccgcaaa aatatcaagg tgcttgaaga taccttccaa agttttggga ttgatgtcaa
    13261 ggtagaacga gcagaaatcg ggccttccgt caccaaatat gaaattaaac cagctgtcgg
    13321 ggtccgagtt aatcgtattt caaatcttgc tgatgatttg gcacttgctc tggctgctaa
    13381 agatgttcgt attgaagcac caattcctgg aaaatcatta ataggtattg aagttcctaa
    13441 ctcagaaatt gcaacggttt ctttccgcga actttgggaa caatctgatg ccaatcctga
    13501 aaacctttta gaagtaccac taggtaaagc tgttaatggc aacgctcgca gttttaactt
    13561 agctagaatg ccgcatcttt tggtagctgg gtcaactggt tcaggtaaat ctgtggcagt
    13621 taatggcatt atttcaagta ttttgatgaa ggcacgtcca gatcaagtta agtttatgat
    13681 gattgacccc aaaatggttg aattatctgt ttataatgat attccacatt tattaatccc
    13741 tgttgtaacc aatccgcgta aagccagtaa ggcactccaa aaagtggttg atgagatgga
    13801 aaaccgctac gagttattta gtaaaattgg tgtgcgtaat atagcaggtt ataatacaaa
    13861 ggttgaagag tttaatgctt cttctgagca aaaacaaata ccgttgcctt taatcgttgt
    13921 cattgtagat gaattggctg acttgatgat ggttgctagt aaagaagttg aagatgctat
    13981 tattcgtttg gggcaaaaag cgcgtgcggc aggtatccat atgattcttg caactcaacg
    14041 tccctctgta gatgttattt ctggtttgat taaagcaaat gttccttcgc gtatggcatt
    14101 tgctgtttca agtggtactg atagccgtac gatccttgat gaaaatggtg ctgaaaagct
    14161 cttgggacgg ggtgatatgc tctttaagcc gattgatgaa aatcaccctg ttcgcttgca
    14221 agggtctttc atctcagatg atgatgtgga gcgtattgtc aactttatca aagaccaagc
    14281 tgaagcggat tatgatgacg cctttgatcc tggtgaagtt agcgacaatg atcctggttt
    14341 ttctggaaat ggcggagctg ctgaaggtga tcctcttttt gaagaagcaa aagccttggt
    14401 cttagagaca caaaaagcta gtgcctcaat gattcaaagg cgtttgtcgg ttggatttaa
    14461 tcgtgccacg cgcttgatgg atgaattgga agaagcaggt gttatcggtc cagcagaagg
    14521 aaccaaacct cgtaaggtat tgcagacaaa ctaaaagaac ctgacgtctc ataatgagat
    14581 gtcaggttct ttttttgtga tgtcaggcca gttgatgaga tgcctatgct aatgtaaaaa
    14641 taaagataac tagaataagg tagaaaataa cggtgactaa aaagccaact cgccaaaata
    14701 gcttcataaa gcgatggtaa gaaaacgact gagttcggat gactagcagt agtgaaatga
    14761 cgatggctag cagcgacatg ataataagat aataaggcag taaattatgg gtgaaaaatt
    14821 ttcctgagac aagcacaatt tctatcgcaa aaagtaaaag tgcgacatct ggaaaattaa
    14881 atcccttttt attaagttta aataaagagg ttacaatcat ggcaaaaaga ggcgttacta
    14941 tcaagaaagc taaggcagtt aatttataga tcatcatagg tctattttat actcaaatcc
    15001 cttacttgta aactgatact tacctgttgt gatagaaact gatctttgag agggaaagag
    15061 cctgcttcaa tttaaatatt gcaaaaggca tcagctattt taacaaaaag acttgattta
    15121 ttttggcaat aagctataat ggtaaggtat gcaatattgc atatctgtgg gaggtaaaaa
    15181 tctcaattac cgccaaaacc acaacaggag gatttttaaa atggctaaaa aagtcgaaaa
    15241 acttgtaaaa cttcaaatcc ctgctggtaa agctacacca gctccgccag ttggaccagc
    15301 tcttggtcaa gcaggtatca acatcatggg cttcactaaa gaatttaacg ctcgtacagc
    15361 tgatcaagct ggtatgatca tcccagttgt tatctcagtt tatgaagaca aatcatttga
    15421 tttcatcact aaaacaccac cagctgctgt tcttttgaaa aaagctgcag gtgttgaaaa
    15481 aggctcaggt acacctaaca ctactaaggt tgcgacagtt actcgtgcac aagtacaaga
    15541 aattgctgaa actaagatgc cagatttgaa cgctgcaaac attgaagctg caatgcgtat
    15601 gatcgaaggt actgctcgtt ctatgggatt cactgttact gactagtcag tcaagaaccc
    15661 ctattacccg cacgacttca tcataagaga tgagtgacgt gggagatttt aaatcaaatc
    15721 gatatgacca cattacaagg agaattttaa aatggctaaa aaaagcaaac aaatgcgtgc
    15781 tgcacttgaa aaagtagata gcacaaaagc gtacagtgta gaagaagctg tagcattagt
    15841 aaaagaaacg aacttcgcaa aatttgatgc gtctgttgaa gttgcttaca acttgaacat
    15901 tgacgttcgt aaagcagacc aacaaatccg tggcgcaatg gtattgccaa acggaactgg
    15961 taaaacacaa cgtgttcttg tttttgcacg tggtgcaaaa gctgaagaag caaaagcagc
    16021 tggtgcagac ttcgtaggtg aagacgacct tgttgcaaaa atcaatggtg gatggcttga
    16081 ctttgacgtt gttatcgcaa cgccagacat gatggctatc gtaggtcgtc ttggacgtgt
    16141 ccttggacct cgtaacttga tgccaaaccc taaaactggt acagtaacga tggatgttgc
    16201 caaagccgtt gaagagtcta aaggtggtaa aatcacttac cgtgctgaca aagcaggtaa
    16261 tgttcaagct cttattggta aagtttcatt tgatgctgac aaattggttg aaaacttcaa
    16321 agccttccac gatgtaatgg ctaaagctaa acctgcaaca gctaaaggaa cttacatggc
    16381 aaacgtctca atcacatcaa cacaaggtgt tggtatcaag gttgatccta actcacttta
    16441 agaaaaaaat tagggcatcg cctaatcata gcaaaaagac aaatttctga agacaaatct
    16501 tcactagagg tttgtctttt ttatggttta aaatggaatg caaacaaaaa agtgtaggcg
    16561 ataataccat gaaaggagtt gattccctta atgagatgtg gtgttaacac atcatgagct
    16621 cacaaaaaat gataaaggaa gtatcggtgg tgttaaaaaa gggattttaa ttgatattta
    16681 ttagcctcta aaatgtcctt tccctattca taattgaggg tttgtggtaa aatagtaagg
    16741 acaaactaag gagaacatag tagtggaacc taaatatcaa cgtatattaa ttaagttatc
    16801 tggtgaagca ttagcaggtg aaaaaggagt tggcattgac attccaactg ttcaggctat
    16861 tgcaaaagaa attgccgaag ttcatgtgtc gggagtacag atcgctcttg ttattggtgg
    16921 aggcaatctc tggcgtggag agcctgcagc agacgctgga atggaccgtg ttcaggctga
    16981 ttacactggc atgcttggaa cggtgatgaa tgccctagtc atggctgata gtcttcaaca
    17041 ttatggtgtt gatacccgtg tgcaaacagc tattcctatg caaaatgtag cagaacctta
    17101 tattcgcgga cgtgccttgc gccatctaga aaaaaatcgg attgttgttt ttggagctgg
    17161 tattggctcg ccttattttt caacagacac caccgcggca ctgcgtgctg ctgagattga
    17221 agcagatgct attttaatgg caaaaaatgg tgtggatggg gtctataatg ctgatcctaa
    17281 aaaagatgct aatgcggtta aatttgatga attaacacat ggtgaagtga tcaaacgtgg
    17341 tctcaaaatc atggatgcaa cggcatcgac cttatcgatg gataacgata ttgatctggt
    17401 ggttttcaat atgaatgaag ctggcaatat tcaacgtgtt gtctttggag aacatattgg
    17461 aactactgta tcaaataaag tttgtgacta aaagttagaa aagaaagaga gtaattatca
    17521 tggcaaatgc aattattgaa actgcaaaag aacgttttgc acaatcccat cagtctttat
    17581 cacgtgaata tgccagcatt cgtgcgggtc gtgccaacgc tagtctttta gaccgtatcc
    17641 aagttgatta ttacggggca ccgacgccat taaatcaatt ggcttcaatc actgtaccag
    17701 aagcgcgtgt cttattgatc tcgccttttg ataaatcttc tatcaaagat atcgagcgtg
    17761 ctcttaacgc atcagattta ggcattacac ctgctaacga tggctctgtc attcgtttgg
    17821 ttattccagc tttgaccgaa gaaacacgta aagaattagc caaagaagtg aaaaaagttg
    17881 gtgaaaatgc taaaattgca atccgtaata tccgccgtga tgctatggat gatgctaaaa
    17941 agcaagaaaa agcaaaagaa atcactgaag atgagttaaa aacacttgag aaagatatcc
    18001 aaaaagcaac cgatgatgct atcaaagaaa ttgaccgcat gacggctgaa aaagaaaaag
    18061 aattgctctc agtgtaacag gattcctacg aaaaatagac gactatagac acaaaaagat
    18121 aggtgaccat ttcagaagcg tttggttttg atttgtccct atttttttaa agaaagaaaa
    18181 aagatatgaa tgatttacta gcaacagtca ttactgggct tattaaagaa gaaaacgcta
    18241 atgactattt tatccaaaaa gaagggttta cctttacctt gtctaaagca gaaggagaac
    18301 gccaggttgg tgatatggtc acaggctttg cctatataga catcaagcaa aaggcacgtc
    18361 tcacaaccaa agagatccgt tcaacacgga caagttatgg ctggggggaa gtgacagaga
    18421 ttcgtcgtga cctaggcgtt tttgtcgata caggaattcc aaataaagaa attgtggtct
    18481 ctttggacgt gttgcctgaa atgaaagaac tatggcctaa aaaaggggat aaactttaca
    18541 ttcgtttgga tgtggataaa aaagaccgta tttggggact tccagcagag cctgaggtct
    18601 ttcaaaaaat ggccagtcct gcatacaata acatgcaaaa tcaacattgg ccagcaattg
    18661 tttaccgcct aaaattaaca ggaacttttg tttacctgcc tgaaaacaac atgttgggct
    18721 ttattcaccc aagtgaacgc tacgcagaac cgcgcttagg tcaggtttta gatgcccgtg
    18781 tgattgggtt tagagaggtc gatcgtacct tgaatttatc gttgaaacca cgttcttttg
    18841 agatgttgga gaatgacgct cagatgattg tcacctatct ggaagccaat ggtggtttca
    18901 tgaccttaaa tgacaaatca agtccagaag agattaaggc aatctttggt atttcaaaag
    18961 gccagtttaa aaaagccttg ggtggattga tgaaagctaa acgcatcaaa caagacgcca
    19021 caggaactga attaatagga taaaaaaact ttgccactgg caaagttttt tgttacaatg
    19081 aagaccatta agtgaagaaa aaggggatat agagaatgga aagagccatt tttgcaggag
    19141 gttgtttctg gtgtatggtg caaccttttg aggagcaagc aggtattttg tcagtcagaa
    19201 gtggctacac aggtgggcat ctcccaaatc ctagctatga acaagtttgt gccaaaacaa
    19261 caggacatac agaagctgtt gaaattattt ttgaccctga agagatcagc tataaggaat
    19321 tagttgaatt gtattgggcg cagacggatc caacagatgc ctttgggcaa tttgaagatc
    19381 gtggggacaa ctaccgtcct gtcatttact atacaacgga aagacaaaaa gaaattgcag
    19441 agcaatcaaa agcaaacttg caagcttcag gacgttttga ccaacccatt gtcaccacta
    19501 ttgaaccagc agagccattt tatctcgcag aggattatca ccaaggtttc tataaaaaga
    19561 atcccaagcg gtatgcacaa agcagtgcta ttcgtcatca gtttttagag gagaactggt
    19621 catgagaaaa tcattttaca gctggctgat gacacaacgt aaccctaaat caaatgaacc
    19681 tgtggcgatt ttagcagatt tggtgtttga tgacaccact tttccaaagc ataccaatga
    19741 ttttgaactc attagtcggt atctagaaga tcaagctagt ttttccttta atttaggtca
    19801 atttgatgaa atctgggaag attatctagc acattaatac gtggataaaa aagagtcata
    19861 tcgactcttt tgaagggtta tcacgttatt attacaaaat gacatcactt tctttcaaaa
    19921 aaatgacaag ctactttttt tcttaaaaat atgctatgat atagcttaga aaaaaataaa
    19981 aaggaagcag agatgattat tactaaaaag agcttatttg tgacaagtgt cgctttgtcg
    20041 ttagcacctt tggcgacagc acaggcacaa gagtggacac cacgatcggt tacagaaatc
    20101 aagtctgaac tcgtcctagt tgataatgtt tttacttata ctgtaaaata cggtgacact
    20161 ttaagcacaa ttgctgaagc aatgggaatt gatgtgcatg tcttaggaga tattaatcat
    20221 attgctaata ttgacttaat ttttccagac acgatcctaa cagccaacta caaccaacac
    20281 ggtcaggcaa cgactttgac ggttcaagcg cctgcttcta gtccagctag cgttagtcat
    20341 gtacctagca gtgagccatt accccaagca tctgccacct ctcaatcgac tgttcctatg
    20401 gcaccatctg cgacaccatc tgatgtccca acgacaccat tcgcatctgc aaagccagat
    20461 agttctgtga cagcgtcatc tgagctcaca tcgtcaacga atgatgtttc gactgagttg
    20521 tctagcgaat cacaaaagca gccagaagta ccacaagaag cagttccaac tcctaaagca
    20581 gctgaaacga ctgaagtcga acctaagaca gacatctcag aagattcaac ttcagctaat
    20641 aggccggtac ctaacgagag tgcttcagaa gaagtttctt ctgcggcccc agcacaagct
    20701 ccagcagaaa aagaagaaac ctctcagatg ttaactgcgc cagcagcaca aaaagctgta
    20761 gctgacacca caagtgttgc aacctcaaac ggcctttctt acgctccaaa ccatgcctac
    20821 aatccaatga atgcagggct tcaaccacaa acagcagcct tcaaagaaga agtggcttct
    20881 gcctttggta ttacgtcatt tagtggttac cgtccaggag atccaggaga tcatggtaaa
    20941 ggtttggcca ttgattttat ggtgcccgaa aattctgctc ttggtgatca agttgctcaa
    21001 tatgccattg accatatggc agagcgtggt atttcatacg ttatttggaa acagcgattc
    21061 tatgcgccat ttgcaagtat ttacggacca gcctacacat ggaaccccat gccagatcgc
    21121 ggcagtatta cagaaaacca ttatgatcat gttcatgtct cctttaatgc ttaattcatg
    21181 ctatcaaaaa aaactcacgt ttaggcgtga gttttttgac aattctgaca gagtaagccc
    21241 tataagaggc tgagatacgc ttttaatacc tgttgttttt ctcacccttt agacagtttt
    21301 aacgttatgt ctaaaagaga tggtttttat tagcgcaaat atgggtttag gggtaaaata
    21361 taggtaatta acctgatatg atcatgataa aggtagaaga atacctatta tttcgtgaca
    21421 actgagcaat ctgcccttac ctttggagtg atgatatcac tttgcttgac tattcaagga
    21481 ggtcttgaaa tgtattatac tagtggtaat tacgaagctt ttgcgacacc tcgaaaacct
    21541 gaaggggtag atcagaaatc ggcttatatt gttggtactg gtttagctgg tttagcagca
    21601 gctgttttcc ttattcgcga tgggcatatg gctggggaac gcattcgtct gtttgaggaa
    21661 ttgcctttag caggtggttc tttagatggt attgaaaagc ctcatcttgg ttttgtgacc
    21721 cgtggtggtc gtgagatgga aaatcatttt gagtgtatgt gggacatgta tcggtctatt
    21781 ccctcactgg aaattcctgg tgcgtcttat ttggatgaat tttattggtt ggataaggat
    21841 gatcctaact catccaactg tcgtttgatt cacaagagag gaaatcgtgt ggatgatgac
    21901 ggccagtata cgctcggtaa acagtcaaaa gaattaatcc atttaatcat gaagacagaa
    21961 gaatctctag gagatcaaac cattgaagag ttcttctcag aagatttctt taagagtaat
    22021 ttttggatct attgggcaac catgtttgct tttgaaaaat ggcattctgc tgtagaaatg
    22081 cggcgctatg cgatgaggtt tatccaccat attgatggtt tgccagattt cacctctctc
    22141 aagttcaaca aatataacca atatgattct atggtcaaac cgattattgc ttacctagaa
    22201 tcacacgacg ttgacatcca atttgacaca aaagtcactg atattcaggt ggaacaaaca
    22261 gctggtaaaa aggtagcaaa aaccatccat atgacggtgt ctggggaggc taaggcgatt
    22321 gagctaacac ctgatgattt ggtttttgtg accaatggtt ctattactga aagtagcaca
    22381 tacggtagtc atcacgaagt ggctaagcca accaaagcgt taggtggttc ttggaattta
    22441 tgggaaaatc tagctgctca atcagatgat tttggtcatc ctaaagtgtt ttaccaggac
    22501 ttgcctgctg aaagctggtt tgtgtctgcc acagcaacca taaaacaccc agctatcgag
    22561 ccttatattg aacgtttgac ccaccgtgat ttgcacgatg gcaaagtgaa cactggcggc
    22621 atcatcacta ttacagattc taactggatg atgagctttg ccattcaccg tcaacctcat
    22681 tttaaagaac aaaaagataa tgaaaccact gtctggattt acggtcttta ttccaatagt
    22741 gagggcaatt acgtccacaa gaaaattgag gagtgtacag gtcaagaaat cacagaagaa
    22801 tggttgtacc accttggggt acctgttgat aaaatcaagg acttagcgag tcaggactat
    22861 atcaatacag ttcctgttta catgccttat attacgagtt actttatgcc acgtgtcaaa
    22921 ggagaccgtc cgaaagttat cccagatggt tcagtcaact tggcctttat tggtaacttt
    22981 gcggaatctc catctcgaga tacggtcttt acgactgagt attctattcg tactgccatg
    23041 gaagcagtgt atagcttctt gaatgtggaa cgaggcattc cagaagtctt taattcagcc
    23101 tatgatattc gtgaattgct caaagccttt tattacctta atgataaaaa ggcaatcaag
    23161 gatatggatt tgccaattcc tgcactgatt gagaaaatcg gacataaaaa aatcaaggat
    23221 acctttatcg aagaattgct caaagatgct aatcttatgt aacatgcctt agagtagtta
    23281 aagctgataa gtttaggctt atcagctttt ttgatgattt atagaaaagt gataaaggaa
    23341 aagtctgacg tgaacgtcac ataagaattt aaaatttgtt ataatgtaag caataaagat
    23401 gaaagaaaga gggactctat ttgcaagagt attctattga cattacctta acccatcctg
    23461 atgatgtttt agctcttttt ggtagcaacg agcgtcattt gaagctaata gaagctcatt
    23521 taggagttat tgttcacgca agaaccgagc gagtccaagt gattggtgat gatgaagaag
    23581 ctgtggaatt ggctcgattg accatcaaag ccctactggt tctagtgggt cgtggcatgg
    23641 tggtcaacac atcagatgtg gtgacagctt tatctatggc agaatctcat cagattgacc
    23701 aatttatggc gctttatgaa gaagaaatca ttaaagataa ctatggtaag gcaatacgag
    23761 tcaaaacatt aggtcaaaaa acctatgttg atagtgtcaa acgtcatgat gtggtttttg
    23821 gtgtaggacc tgcagggaca gggaaaacct tcttagctgt tacgcttgca gtcactgctc
    23881 tgaaaagagg acaggtaaag cgtattattt tgacgcgtcc tgcggtagaa gctggtgaaa
    23941 gcctaggttt tttaccaggc gatttgaaag aaaaggtgga tccttacctc agacctgttt
    24001 acgatgccct ttatcacatt ttaggcaagg aacaaaccac acgcctgatg gaacgagatg
    24061 tgattgagat tgctccgtta gcttatatgc ggggtcgtac tctggatgac gcttttgtta
    24121 tcttagatga ggctcaaaac acgaccatca tgcaaatgaa gatgtttttg acccgtcttg
    24181 gttttaattc taaaatgatt gtcaatggag atacgagtca gattgacctc cctcgaaatg
    24241 tcaagtctgg tttgattgat gctactcaga aattacaagg cattaaacag attgattttg
    24301 tctatttctc tgctaaggat gttgtccgac atcctgttgt ggctgatatt atcaaggctt
    24361 atgaaacgtc ctcagaggag atgaaggact tactaaaacc taaggctagt gacgttgaag
    24421 ctgattacca gccaggcttg acaaaatacc ctgtgattgg tcaagagcac taagattttt
    24481 acgatgatgt ttaatatggc ctttttaagg aggattctat ggatgattta accaaagcta
    24541 ttatggcaga tgaggctaat ctctcctata ccgaaagagg tatttttcct ttatatgatg
    24601 ctcctcaaac agctaggatt attattgttg gccaagctcc tggaatagta gctcaagaaa
    24661 ccaaacttta ctggaatgat cgtagtggca tccgcttacg agactggcta ggtgtggaca
    24721 atgacacctt ttaccactca ggattgtttg gcattatccc tatggatttc tattatcctg
    24781 gtaagggaaa atccggtgac ttaccaccta gagaaggttt tgcggctaag tggcacccgc
    24841 cattaagagc attgatgcca gaagttgaat tgacgatttt ggttggtcgc tatgctcagg
    24901 acttttacct tggtaacaag gcttacaaaa ccttaactga aacagtccgt cactttgagg
    24961 attacttgcc agactatttt ccacttgttc atccttctcc aagaaaccaa ttgtggttag
    25021 ctaagaaccc ctggtttgag caggatttat tacctatctt gcaaaaacgt gtggaggcaa
    25081 ttctaaccaa ataggccctg atttacctag cattcccatt tttaaggggc ttgtggtata
    25141 atgggtaaat aacaaatgct gattgaaagg ataagggaaa ttatctgtca gataataatg
    25201 ggtgcccaac ataccaaatg aggtgggcag cccttcccta acaattaacc ttatgtatat
    25261 cgagatgatt gacgaaacgg gacaagtttc gcaagagatt atggagcaaa cacttgattt
    25321 gctcaatttt gctgctcaaa agacgggcaa agaagaaaaa gaaatgtctg tgacctttgt
    25381 gaccaatgaa agaagtcatg aactcaactt agaataccga gatactgatc gtccaacaga
    25441 tgtgatttct ctagaatata agccagaaac gcctatttta tttagtcaag aggatttggc
    25501 tgctgatcct agtttggcag agatgatggc agagtttgat gcttatattg gcgaattgtt
    25561 tatttctatt gacaaggcgc gtgagcagtc ccaagaatat gggcactctt ttgagcgtga
    25621 aatgggattt ttagctgttc atggattctt acatattaat gggtatgatc actacaccct
    25681 tgaagaagaa aaagagatgt ttaccttaca ggaagagatt ttgactgctt atggccttac
    25741 acgacaataa taccaccaaa cggaaatgga aaaacagaac cattacctca agcttggagt
    25801 ttgccttgac aggggttttc acagctttta aggaagaacg caatctcaga agccaccttt
    25861 tgtctgcctg tttagcttgt gttgctggtt tattttttag catttcagcc atcgaatggt
    25921 tatttttact tctagccatt tttttggtca ttaccttaga aattgtcaat tctgctattg
    25981 aaaatgtagt tgatttggct agtgactatc acttttctat gctagctaaa aatgccaagg
    26041 atatggcagc aggtgctgtt ttgatgattt ctggctatgc tgtcttgaca ggacttatta
    26101 tttttattcc taaaatttgg aatattttcg ttcattaaaa agaaaggaat gggctatcct
    26161 atgtgatgat ggcataggag aataaggaaa ggacaagacg gttaagctta gtgacaagct
    26221 tgatctgaag ccctaccgtt aaggcccgta attatctatg tttaaatcag gctttgtagc
    26281 cattcttggt cgtccaaatg ttggcaaatc caccttttta aatcacgtca tggggcaaaa
    26341 aattgccatc atgagtgaca aggctcagac cacccgtaat aaaattatgg gcatttacac
    26401 cacagaaaca gaacaaattg tctttattga tacgccaggt attcataaac caaagaccgc
    26461 ccttggtgat tttatggttg aatctgctta tagtacttta cgtgaagttg agacagttct
    26521 cttcatggta ccagcagatg aaaaacgtgg taagggtgac gacatgatta tcgagcgcct
    26581 aaaagcagct aagattcccg ttatcttagt catcaataaa attgacaagg tgcaccctga
    26641 ccaacttttg gcacaaatcg atgattttcg tagtcaaatg gagttcaaag aagtagtacc
    26701 gatttctgcc ctagaaggta ataatgtgcc aactttgatc aagttattaa ctgataatct
    26761 tgaagaaggt ttccaatatt tcccagaaga tcagattacc gatcacccag aacgttttct
    26821 tgtgtcagaa atgatccgtg aaaaagtgtt acacctcacc cagcaagagg ttccacattc
    26881 cgtagcggtt gtggtcgaat ccatgaagcg agatgaggaa accgacaaag tgcatatccg
    26941 tgcaaccatt atggtggagc gcgatagcca aaaaggtatt atcatcggta aacaaggagc
    27001 catgctcaaa aaaatcggaa aaatggcacg tcgtgatatt gaactcatgt taggtgacaa
    27061 ggtttactta gaaacgtggg tcaaagtcaa gaaaaactgg cgggataaaa agcttgattt
    27121 agctgatttt ggctataatg agaaagaata ctaacatagt aaagcgagat catatgccac
    27181 aagactacat ttcttatatc cgctccaaag ttgggcatga taagattatt cttaactttg
    27241 ctggtgggat tttgaccgat gatgacggca aggtgctgat gcagttgcgt ggtgataaga
    27301 aaacctgggc tattcctggt ggcactatgg aactgggtga gagctcttta gagacttgca
    27361 aacgtgagtt cttagaggaa accggaattg aggttgaagc tgttcgctta ctgaatgttt
    27421 acactcattt tgaggaagtt tatcccaacg gtgatgctgt tcagactatt gtctttatct
    27481 atgaactaac ggctgtttct gatatggcta ttgataactt tcataatgag gaaacgctca
    27541 agttacaatt tttctctcat gaggagatag cagagttaga gagtgtctca gccaaacatc
    27601 gcctgatgtt agaggaatat tttagtgata gctttgctat ggggcattaa gaactctcta
    27661 ggacagttgg agtatttttg gagattggct tgttaaaaaa atttttgaaa gacgatattc
    27721 gagagtttat gctctctttt tttgcttcca atttttctga ttaagtctga ataattttgt
    27781 taaatgttaa caggattttc tttccaattg atactagcag acatcaaaca tttgttttag
    27841 agttatgacg aattgccacc tagccatgca tgacttaggc aagttgaacc taactgtgac
    27901 aatagacttg ggaggggtga caagaatcat ttcctaaaag aattgatgaa gtgcctatcc
    27961 attattcgaa ttgtaaagtt cgtctaagta aagttgaatt tggtctcgaa tggaccttaa
    28021 tgatttatga cttgttgtgt caataacacc aaaaactcga taatgatttt taaatatttc
    28081 ctcgtaatta atatgatttc cgattagaat aatatgagca tgaggataac gcatacgtat
    28141 ttcgtttaga aggtcat
//
LOCUS       DAUELZ010000010        28108 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.010, whole genome shotgun sequence.
ACCESSION   DAUELZ010000010 DAUELZ010000000
VERSION     DAUELZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28108
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.010"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(96..194)
                     /gene="ffs"
                     /locus_tag="VHI09_000352"
     ncRNA           complement(96..194)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHI09_000352"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            394..1626
                     /locus_tag="VHI09_000353"
     CDS             394..1626
                     /locus_tag="VHI09_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEQ6120258.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQAQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEQLSLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(2240..2755)
                     /locus_tag="VHI09_000354"
     CDS             complement(2240..2755)
                     /locus_tag="VHI09_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEQ6120259.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(2755..3534)
                     /locus_tag="VHI09_000355"
     CDS             complement(2755..3534)
                     /locus_tag="VHI09_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ6120260.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PEGINSKNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(3674..4213)
                     /locus_tag="VHI09_000356"
     CDS             complement(3674..4213)
                     /locus_tag="VHI09_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEQ6120261.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALFISGVLLVDVMGAIWLAAYTGMPLMTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTKQ"
     gene            complement(4217..4528)
                     /locus_tag="VHI09_000357"
     CDS             complement(4217..4528)
                     /locus_tag="VHI09_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120262.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LADHAFVPTGHQETSPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(4745..5887)
                     /gene="tgt"
                     /locus_tag="VHI09_000358"
     CDS             complement(4745..5887)
                     /gene="tgt"
                     /locus_tag="VHI09_000358"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEQ6120263.1"
                     /translation="MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVVKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            6106..6969
                     /locus_tag="VHI09_000359"
     CDS             6106..6969
                     /locus_tag="VHI09_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEQ6120264.1"
                     /translation="MSIKAIKSQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGLILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     GNLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGSSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDKVKEGTYLGAIAVIKTSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
     gene            complement(7071..7538)
                     /gene="perR"
                     /locus_tag="VHI09_000360"
     CDS             complement(7071..7538)
                     /gene="perR"
                     /locus_tag="VHI09_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEQ6120265.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQPDF"
     gene            complement(7590..8045)
                     /locus_tag="VHI09_000361"
     CDS             complement(7590..8045)
                     /locus_tag="VHI09_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEQ6120266.1"
                     /translation="MIYSFQNPSEAVLKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEAQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNH"
     gene            complement(8232..10874)
                     /gene="polA"
                     /locus_tag="VHI09_000362"
     CDS             complement(8232..10874)
                     /gene="polA"
                     /locus_tag="VHI09_000362"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEQ6120267.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPEEFREQFPYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTAAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKTSKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAVISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     REAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRTTLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDKHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQAKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            complement(11005..12732)
                     /locus_tag="VHI09_000363"
     CDS             complement(11005..12732)
                     /locus_tag="VHI09_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEQ6120268.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMAMTSGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMEAVMLDINKGMSPEAAAKKWVEAN
                     KSKVSSWTK"
     gene            complement(12748..13944)
                     /locus_tag="VHI09_000364"
     CDS             complement(12748..13944)
                     /locus_tag="VHI09_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ6120269.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILKNTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            complement(14432..15523)
                     /gene="ulaG"
                     /locus_tag="VHI09_000365"
     CDS             complement(14432..15523)
                     /gene="ulaG"
                     /locus_tag="VHI09_000365"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEQ6120270.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLDHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(15776..17422)
                     /locus_tag="VHI09_000366"
     CDS             complement(15776..17422)
                     /locus_tag="VHI09_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEQ6120271.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INKALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            complement(17747..18451)
                     /locus_tag="VHI09_000367"
     CDS             complement(17747..18451)
                     /locus_tag="VHI09_000367"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEQ6120272.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVAKMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            complement(18453..19316)
                     /locus_tag="VHI09_000368"
     CDS             complement(18453..19316)
                     /locus_tag="VHI09_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEQ6120273.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPATEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLA"
     gene            complement(19321..19983)
                     /locus_tag="VHI09_000369"
     CDS             complement(19321..19983)
                     /locus_tag="VHI09_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEQ6120274.1"
                     /translation="MTHIPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     IKEERGDRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIKRI
                     WG"
     gene            complement(20090..20575)
                     /locus_tag="VHI09_000370"
     CDS             complement(20090..20575)
                     /locus_tag="VHI09_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ6120275.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVQLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKNSPYLEGLDLD
                     A"
     gene            complement(20698..20976)
                     /locus_tag="VHI09_000371"
     CDS             complement(20698..20976)
                     /locus_tag="VHI09_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ6120276.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            complement(21047..22480)
                     /locus_tag="VHI09_000372"
     CDS             complement(21047..22480)
                     /locus_tag="VHI09_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ6120277.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPIFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSNVELIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGILQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            complement(22787..25288)
                     /locus_tag="VHI09_000373"
     CDS             complement(22787..25288)
                     /locus_tag="VHI09_000373"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEQ6120278.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIDKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGNEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            complement(25500..26639)
                     /locus_tag="VHI09_000374"
     CDS             complement(25500..26639)
                     /locus_tag="VHI09_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEQ6120279.1"
                     /translation="MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            27123..27400
                     /locus_tag="VHI09_000375"
                     /pseudo
     CDS             27123..27400
                     /locus_tag="VHI09_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27543..27710
                     /locus_tag="VHI09_000376"
     CDS             27543..27710
                     /locus_tag="VHI09_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120280.1"
                     /translation="MKKKLSLFMIATAAVASLSLATLGSHQTVSANDASYSNGFYGHT
                     GPDSWLLYTVW"
BASE COUNT         8442 a         6103 c         5293 g         8270 t
ORIGIN      
        1 gacaaaacta gctatatcaa tacaataatt tattagctta taagagctca ataaaaaata
       61 aaccgaactc atcacatcaa gttctttgct catttaaaat gcacaaaaaa agagcacaca
      121 cccgaaaatc gcttagggct gctggattcc tcccctgacc cacttcacgc acaagtgttg
      181 ctccgcctat tattatatca tattacggac agtttgcaag ttttccaact aagtaaaatc
      241 actttcaagt gagaatagct tcattttggg acaagatgga ctgaatacct agaaaaatca
      301 tgaccctttc caaacacgat tttgcttttt gaaaatcttt ccctctcccc attccagcta
      361 ttctctatgt tataataaga ttattagatt agaatgggag ttatgatgaa acaaaagatt
      421 aaaatcttaa cagtaattgg ccttatgaca gttggtatgt ctgcgtgtca caacactagc
      481 aaaccttcta ataccgattc tgttttttct ttaaccggaa aaaagcgcca acaaatcgtc
      541 aaacaagtaa gacaacggta ttatttccaa caattatcca aaaccgaaca agaaaattac
      601 ctcactttat acgatagtct agcacaattt cgtgaaatca tcagtttgac gccggcttct
      661 aaaaaaagct taattaaaac gattgacgcc tttgtgatgg ataatcccga attttactgg
      721 attacatctg ccgattatcg atttgagttt tctgatcaaa cggtctttgt cacctttcct
      781 atcccagaag atgccaagaa cgtttatcaa gacttgcaag caattggtaa tgacatcgtc
      841 gcaaacacgc catcaaagga tcgctatgag caggtcaaat atttttatga ggtcatcatt
      901 cgagacacag actacaataa aaaagccttt gaagcctatc aatcaggcag tcaagcccag
      961 gtcgcttcta atcaagacat taaaagtgtt tttattgatc atttatctgt ttgtaatggc
     1021 tatgcccaag cctttcagtt tctttgtcaa aaagctggca ttccagtcgc ctatatccgt
     1081 ggaactggta catctcagca acctcagcaa tcttttgcac atgcatggaa tgctgttcaa
     1141 attaataaca cttattatgg cgttgatgtc acttggggtg atcctgtttt tgataaccat
     1201 ctctcacatc aaaagcaagg aactatcaat tacagttttc tatgcctacc agattatcta
     1261 atggccttat cacatcagcc aagcaaagac attgctttta atactaaaga acgttttgaa
     1321 aatgtttgga ctataccttc ttgtacagat gattctttgc tttattctaa acgtcatcaa
     1381 agctatatct caacgtttga cagtgacgcc atccttgcaa gccttgaaaa tcagttgtta
     1441 aatagacaag agcaactatc tctacaattt gctcatcaag atgattatca acagatggta
     1501 actgatttaa cgacgaatca aacaggttat cataaccttt tcaaccagta ttggaataat
     1561 tatacgggct ttacctatgg actcttacca gagactctta gtatttcatt tgctagtagg
     1621 aactaggttt agacacatga aacacaaagt taagatgatt atctttgtca aacattacaa
     1681 gaaattgata tactaacaca attgaaggcg attattattc taaccgtctt caatagatat
     1741 aaccttttca gattgagcac atctaatgct tgtaacatta gcaacagtga tgagctcttt
     1801 tttaatgctt tagcaaaata tatcagctaa gacaaaagat taaataactc aagccatgcc
     1861 tataagtcac ttgtttttaa tccctgtgtc agtgtcaatc tacgccacag ataactgaca
     1921 gctctgagcc ctagaagtct gaacttgata agtttactaa aaacaaaaat aaggtgagcc
     1981 attactatct gtttttagat agtaaatctt tttaagaatg actaactcat ctagtcatca
     2041 cgtgtttgat ttttcgattg ttttcacctc gattgggtct gaaaaagagc gtttctagta
     2101 cttggaaacg cccttttgta tattctatct tcgtatgttg aatctaacaa ccattggaaa
     2161 aatggtctaa cgtttcactc attattttga caggttaccc ttcaccaaaa atgcatgtga
     2221 taacaagctc cttaaaactc taatcaaagg gatctgactg ttccttgatc aaatgcttag
     2281 ctattttttt gcgctctcgc ccttggcgga aaaaggtttg catgatgtta gcacagtctg
     2341 ctgctagcag gcctcgttca acttgcacgc ggtggtttaa gcgctcatct gtcaaaatct
     2401 ggtaaaggct gtctgctcca ccaaattttt gattgctggc tccataaata acatgcggaa
     2461 tccttgctag cccaatagca ccactacaca tcacacaagg ctcgatggtc acaaagagtg
     2521 tggtgtctaa taaacgccag ttgccttcat gagcgttagc ctcgttaatg gccatcattt
     2581 cagcgtgcat aatggcttgg ttactctctt cacgcgcatt gtgcccacga ccaataatct
     2641 caccatcctt gacaatgaca cagccaatgg ggatttcagc tttctgtaag gatttttcgg
     2701 cttcttttaa agcctcttgc atgaaataag tttgctcttc taaactatat ggcatcaaga
     2761 aagactcccc aagttccaag atttgtgtgg taaggcaagg tcaacaggaa tattacctgc
     2821 ataggcctgt gtttcttgcc tcaacacttc taaatggttt ttggaattaa taccttctgg
     2881 aggaaccggt ggcatgtggg tcaagacaag cttcttaaca cctgcctggc tagccattag
     2941 tcctgcttcc ttactggtca aatgggccat atgattttca ttgccttcgt ataagtaaac
     3001 atccgctaag aaaaggtctg cttcttttgc gaagtctgcc aatccttcaa aataaccact
     3061 gtctccagta aagacaagta cctgaccagt cgctctttct acaattcgaa aggcataaca
     3121 cacaactgga tgaactgttt taagaaaggt aatgtcaaaa ggaccaatct gttcaactcc
     3181 agtaacatca taggctctac cttctgaaac atcaggaatg cttaacttag caaattcgtg
     3241 tcggtcctca tcatgcccat aaatgggcaa tactttaggc tgccacaggt gtttaggata
     3301 aagttgaaaa taatggcgca acacgcctaa atcggccacg tggtctggat ggtaatggct
     3361 aataatcact gcatccaaat cgagaggact gatttctttt tccagctcat tgagcgctcg
     3421 actaccagca tccatcaata actgaaagcc gtcatgtcct gtgaccaaat aagaggttgt
     3481 gcctgcatct tgatagggat aggctcccca agaccctaag gtggttaatt tcatctcaaa
     3541 cccttctttc ttgctttaac tcttattata acaaaaatta gtgtcaaaga ggattgagac
     3601 ccaccaaaaa caggtcctag cagataaatc tgccaagacc tgttcaacac aacttaacta
     3661 tttgatgatt agattactgt tttgtattca aaaaactatc tttgtattta acagcgataa
     3721 tggtcgcaat gattgctttg atggtatctc ctggaataaa gaccaagttt gataacagag
     3781 aagtcatcaa cggcatccct gtgtaagctg ccaaccaaat cgctcccatc acatccacta
     3841 gcaagacacc agaaatgaat aaggctaaga aagcaagtgg cgtgcttttt gagaaccagt
     3901 tgcggtaaag taatgaaaag acaataggca caagaaggta ggcaataaca tagccagcag
     3961 atggccccat caatactgga atggttgttc ttgagcctga gaaaacaggt aagaaaaggc
     4021 caatcactaa aaataagaaa acagataagg ttcccttttt accacccagc atcaatccag
     4081 ccaacatgac acctaagttt tgtaaaacaa taggaactgg gataaagcct agcgggatag
     4141 caggaataaa gcctaaaata ataatcaaag tggtcatcat agccacctta acaagttctt
     4201 tggttgtaaa catctgttac gactccttta aaaaatagat atccttgtgc ctatgacagg
     4261 ctggattaaa gggactttga caataaggac agcatccaca accatactca gctaggctca
     4321 agagctttcg acaatgccca caaataacag gagatgtttc ttgatgtcct gttggcacaa
     4381 aggcatgatc tgctaactgg tcatgacaat ggtagcaagc ataataagtc tgacaaaaag
     4441 cacatttgag cccaacaata tcaagcgggg tatgataatg taggcaccga tactcttggt
     4501 caaggtcaat tccaaaacag tccgtcatat ctcatcctct tgtaatagtt taatcaaaag
     4561 cttatagttt gtcaactcta tttttaaaaa agggtaactg ttttctgaaa aagaacacgt
     4621 aaaacaagaa ctttccttta aaaagtgacc tatcagttga aagcaatagg acatccgtct
     4681 atacaaaaaa actgaaacca aagacgatag atcgccctca gttccaggat catagcattt
     4741 ttaattagaa attacggttt gatttattat aaccataccg ttcaagaaaa tcttggcgga
     4801 attcaagtaa gttatcgtcc ataatagcct gacgtacttt tttcatcaag ttaaccaaga
     4861 aataaaggtt gtggtagctt gtcaaacgaa taccaaaagt ttcgtccgct tttagcaagt
     4921 gacgaatgta ggcacggcta tagttttgac aagtgtagca gtcacagtca tgatcaagtg
     4981 gggtaaaatc ttcagcgaat ttggcatttt taaccacaag acgcccctca ctagtcatgc
     5041 aggtcccatt acgagcgata cgcgttggca agacacagtc aaacatatcg acaccacgaa
     5101 tcacaccatc aatcaagcta tctggtgctc ccactcccat caggtaacga ggtttatttt
     5161 ctggtaagag gggagtcgta aagtcaagga cggcattcat ttcttcatgg ctctccccaa
     5221 cagctaaacc accaatagaa taacctggga aatccatagc caccaaatct gccgcagact
     5281 gacgacgaag gtcctcaaat ccagctcctt gcacaattcc aaataatcct tggtcatgag
     5341 gacgacgatg ggcttttaag ccacgttctg cccaacggct agtacgttca atggattttt
     5401 tcacgtaatc ataaggttgg taaaattgcg ggcactcgtc aaaactcatc atgatgtctg
     5461 agccaaggtt attctgaatg gaaatggctt tttcgggtga tagaaacatt tttgagccat
     5521 tcaagtgatt tttgaaagtc actccttctt cagtgatgtt tcgtgaatcg gctaaagagt
     5581 aaacttgaaa accacccgaa tcggttaaga ttggctgatc ccagttcata aacttgtgca
     5641 gaccaccaga gcgagcaatc aactcatctc ctggacgcaa ccagaggtga taagtgtttg
     5701 ataaaataat ccctgatcca attgctttta actcttctgg agactgtgtt ttaacagtag
     5761 cctgagttcc cactggcata aacattggtg tcggaaaggt cccgtgaggg gtgataattt
     5821 cgcccaagcg tgcaccggtg tgcttttctg ttttgataag gcgatactta atcggataat
     5881 ctgtcatgtg ctttcttcct tgctatcaag ataaacagtc ttgagctttt ttgatctgcc
     5941 tgagtttcca tcataatgaa gggcttcaag atgacatgtt gttctagcgc ctatcatttt
     6001 atcaaaaaat ccataatttg tcatgctttc ccaaaaaagt atagtcaaaa taatggttag
     6061 ctgtctcatt ttgttttatg gtataatgaa ataaaggagt ctgttatgtc aattaaagca
     6121 atcaaaagcc aggctagaga cacgttaaaa aatctctctg gcaagtattt actattttta
     6181 attccaaccc ttctctttat gtttcatttc ggtattgaga ttcatcaagg ttacgtccta
     6241 tctagtggta ttgaagtctc gctagcggct agttatttcc cattactact aggacttatc
     6301 cttagccttt tcattttatc agcatccttt acgatgattg atgttgttag gcattttcgt
     6361 cagaaagttt cttttgccga atcaacaaca gccttctcaa aagaattctt tgggaacctg
     6421 ctggttttag cgatcactaa gtggctattc tttctcatct ggtcactaat ttggtttttt
     6481 ggcctcttta ttttcctttc cggactttca gcgtttttgg tcaatgctaa atcaggtagc
     6541 tcaacagtca tttcactgat ctttttacta tttggagcag ttttgtcctt aattggtttt
     6601 ggtatttata tcaaccgtta ttacgcttat agtctatcag aataccttct ctacgataag
     6661 gttaaagagg ggacttatct aggagctata gccgttatta aaacaagtgt agctatgatg
     6721 aaaggttata aatggaagct ttttttctta caacttagct tcaccggttg gtttctactg
     6781 aatattgtta cttttgggtt acttaatatc tatcttttac catattttac aacagccaat
     6841 gtcatttttt atgatcaact caagaaacgt ttcaaagaca aagatgaccc aattgaagga
     6901 gaacatttaa gccttcatca gcttaataaa aagcctactc ctatgatgta tgacgatcaa
     6961 aaagactaaa taaatcctcc gtccataaaa atagccctca gccaaaatga actgggggtt
     7021 tttgatttgt aaaggcacta tatttataat gctttattta agggttaact ctaaaaatca
     7081 ggttggtctt ttgcttggca atccggacaa atcccgtaag caataactgg gatacgcgtg
     7141 accttataac ctgtttgctc atgggcttct ttggcaatat ccataacatc aacatccata
     7201 aagtcagcaa tcttcccaca tatttcacaa accacattga cgtgttgatg gcccataaag
     7261 tcatagtagg tggttaaatc attgctgatt ttcaattctg agacaaaacc ttcatcaact
     7321 aaaactttca aattattata gactgtggca aggctcatgt taggaaagtt aggctgtaag
     7381 tcacggtaaa ttttatcggc actagggtgt tctgtggact gaatcatgta agaaataatg
     7441 gctttacgtg tctctgtaat tcgaatatgt ttttcccgta gatgctctag gacattttca
     7501 taggcatcta gggcttgctg atgtgaatga atgtccataa gctgctactc ctatcctttt
     7561 ttcctttatt atcacgcacg tctctagttt caatgattcg tgatgcattc tggtttagcg
     7621 actaattgca aatagtctat ttttagacag cgattcatca caatagcctc tttgcctgcc
     7681 gaccgtaaaa tggtttgtgc ttcttggttt tcaaggccta attgcgccca aaagactttg
     7741 gcctgacctg ctaaaaagtc tctggcaact tctggtaaaa actcacttct gcgaaagaca
     7801 tctacaatgt caacctcaaa gggaattgcc ttgatactgg catagacctt ctctcccaaa
     7861 ataagctgac ctgctaattt gggattgact ggaatgatcc gataatccat agcctgcata
     7921 aatttggcga caccataagc tgctgtgtct ttgcggtcag aaagaccaac aaccgcaatg
     7981 gttttggctg actccaagta agcttttagg acagcctctg agggattttg aaacgaataa
     8041 atcacttgtt cccctatcta tttaataacc tctacagctg ttcaatatag caggataact
     8101 cctaaagcat ggcttcaatc acttttaacc ttatccgtca aaaagctcaa agcagcttca
     8161 tgacttctcc tacaattttt tagtttatta taacataagt tgtcaaaatg agaaataaca
     8221 agtaagaaaa cttatttggc ttcgtaccag ctctgcccag tactttcatc gacacataaa
     8281 ggaacagcta aatcaacagc agcttccatg gtgtctttga caagtttttt tatagcagtc
     8341 aactcatcat ttggcacttc caaaacgatt tcatcatgca cttgaagcag cattttagct
     8401 ctaaaaccac ctgcttgcaa ggccttatcc agattaatca tagcgatttt gagaatatca
     8461 gctgcgcttc cttgaatggg cgagttgatg gctgtacgct ccgcaaaaga gcgaacgttg
     8521 aaattacgag agttgatatc cggtaattca cgacgacgct taaagagggt ctctacatag
     8581 cccttgtctt tagcctcacg caccacattt tccatatagg ctttaatccc aggatagcgt
     8641 tcaaaatagg tatcaatgta agacttggcc tgtttacggg tgataccaag gttatttgac
     8701 aaaccaaaat cagaaatccc atacacaatt ccaaagttaa cagccttggc attacggcgg
     8761 tcattggcag taacatctgc tgccttgtca atcccaaaca cacgcatagc cgttgaggta
     8821 tgaatgtctg ccccttcatt aaaggcagca atcaaatgct tatcccctga aatatgtgcc
     8881 agtacccgca attcaatctg tgagtaatct gaactcaaca agacggcatc ttcatgagat
     8941 ggggtaaagg ccttacgaat caaacgcccc tgttccaaac gaatgggaat gttttgaaga
     9001 ttagggtcca cactagacaa acgccctgtc tgggtgagat cctgcacata gcgggtatga
     9061 atcttgccat cagccagaat ataatcctgt aaaccaatca cataagtgga ttgcaattta
     9121 gtaatctggc ggtaatccaa aattttagcc acaattggag caatcggtgc caaacgttcc
     9181 aaaacatcaa cagctgtaga ataacctgtt ttggtcttct tagtcatctc aagaggtagt
     9241 tgcatcttct caaacaagat actgcctaac tgtttaggcg aattgatatt aaattcttga
     9301 ccagccatat cgtaaatctc ttgggtgagc gcttcaataa tgaccttgtt ttgctctgcc
     9361 atatcttgca aagtcgtcct attaaccttg atcccttcaa tttccatttt agcaaggaca
     9421 ttagccaaag gcaattcaat attctgatag aggtctagtt gctcatgggc agtgagcttg
     9481 tctagcattt gagacttgct atctaataat actttgacct tacgagctaa gtgttctagt
     9541 aagacgtctt tatcaggaac agcccgttta gcacctttac catagaccgt atcgtcttct
     9601 tccaaagaga tgtcagtaaa aatgcgggca atggtgctaa gctcattgtc ttctacagtt
     9661 gacagaaggt aattagctag tctagcatca tagctaggtg ctaccaactc aattcctaag
     9721 tggctcaaca agaccttgct acgtttaaaa tcataagtgg caatgggttt ttggaagacc
     9781 tgcttaaaac tatccgtcgc taataggcta aggtctgtgg atgcgtaaat ctgctcacca
     9841 tgtccccaag caaaaccaat gatggcctca cgatggtaat tatcccgaag cgtttcaaag
     9901 tagaagatgt cttctgcgct aaacaaatca gcagacacat tggtcacttc ttgataggaa
     9961 atgacagcta ctggctcttg aggcatctgg ctagcaagac ctttcttgag ttgcacaaaa
    10021 tccatctcat cgtaaaattg agagaggcta gcaacatctg gaccattgta gacaatatca
    10081 tctaagccaa ttgtgatagg agatgctgta ttgatagtgg cgagcgtttt agataggaaa
    10141 gcttggtcac ggtcattgat caagttttct ttcatttttg aggtcttgaa tccatcaata
    10201 tgctcgtaaa tgccttccaa actaccaaat tcatgaagga gcttaaggcc tgttttttct
    10261 ccaatttttg tcactccagg aatgttatcc gatttatctc ccatcaaggc tttgaggtca
    10321 ataaactggt ttggcgtcaa gcccattttt tccatgaggt aagccgctgt gaattcttca
    10381 aattcggcaa cccctttttt agaaatttca accacggtat tttcgtcagt taactggatc
    10441 aaatctttat caccactgac aatggtcaca tcaaaaggca cttccgtgcg ctccgccatt
    10501 ttatcaagcg tcccgataat atcgtctgct tcgtagtgtt ccaactcata ataagcaatc
    10561 ccaagggctg tcaacatctc acgaatataa ggaaactgct cacgaaattc ctctggtgtc
    10621 ttggcgcgac ccgccttata atcggcatac atttctgttc ggaaggtggt tttgcccgca
    10681 tcaaaagcca ccagcacgtg agtaggctgg actcttttca tcatgtggtc tagcatcaaa
    10741 tgaaagccat aaatggcatt ggtatgaaga ccgctatgat ttttaaagcg gtcaatttga
    10801 ttgtaaaggg caaaaaaggc acgaaaggca acagaggagc catcaattaa tagtaattta
    10861 ttcttgtttt ccataagctt attataacac agagggacgg gcttttctcc tttaagccat
    10921 tggttttgct gataagtaaa tggttaacct aaaaagccta ggaaaattcc tagacttctc
    10981 tatttgctat ttagacaaaa gcacttattt tgtccaactt gatactttag atttgtttgc
    11041 ctctacccat tttttggcgg cagcttctgg actcatgcct ttattgatgt caagcatgac
    11101 tgcttccata tctttttggg tccagtggaa cttatcaatg attttgtaga catttggaag
    11161 gtcttttttc aagcctttac gagcaatggt attgatgttc tcagtagagc caaaggtttc
    11221 tttaggatct ttaaggtact tcaagtcata cttggcaaac atccagtgtg gtgaccaagc
    11281 tgtcacgaca ataggatctt ttttcttgat cgcttgatcc aaggatgttg tcatggctcc
    11341 tgttgatgcc gccactaact cccaactaga tagattatga tactctttta gagtcttttt
    11401 ggctgctgcc ataatacctg cacctggttc gatccctgtg attttttgat cggcttgttt
    11461 gcttaaatcc tcaatactat taacatctgt catgtattta ggaacagcta acccaagttt
    11521 ggtccctttt aggttaggcc ctaaatcatc taattttgac ttgtatttct gatattgttg
    11581 gccatgggtg actggcaacc aagcactagt tgaaaaatca gcgttcccat ttgcaacggt
    11641 ttgccacata acggcattgt caagcggagt caaggtcaca tggtaacctt cgtttttcaa
    11701 aacttctgca atcacatgag ttgaggcaac ctcagaatcc cactgcacgt aagcgatgtt
    11761 aacagtttca cccttatctg ccataccaga agtcatagcc ataataccgc gtcctaaagc
    11821 agctatcaaa aagacagcaa gggctgctag accaatccat ttattggttt tgccagcttt
    11881 ggctttttct tgaggtttgc tattgaacaa ctgtgtcata cggtcaagca caatcgccaa
    11941 gataaccaag gcaagaccac tgacaaatcc actaccaata tcagcgtgtt gaagggctga
    12001 caagacttca cgtcctaagc caggagcacc aatcatggag cctgttacta ccatggaaag
    12061 ggctaacatc atggtctgat taacacctgc cataatagta tttttagcta aaggcaattc
    12121 cactttaaag agcttttgtt tcccagttga cccaaaggca tctgaggcct caatcaattc
    12181 tgttggaatg tcacgaatgg ccaaatttgt aaaacgaacc gttggaggca aggcaaagat
    12241 gactgaggca aacactccag gcaccatacc aataccaaaa aaggcaacgg caggaatcaa
    12301 gtaaacaaaa gccggcatgg tttgcataaa gtccaaaata ggattcacaa tctgacggac
    12361 agtggcattt ttagccatcc aaatccccag cggaatacca ataagtaccg aaatcaaact
    12421 agctactaac accagagtaa aggtattcat tagctgcttc cataaccctt gattgtaaat
    12481 aaagagcaat cctaacaagg taaaggttgg caaaggccat tttttcttag caaggaaaaa
    12541 catccctgct gttactagaa caataaaaag taacgggtgg ataaataaaa gtgttttggt
    12601 catccaatcc atcaaaaagc taccaactac ttgcataata tcaaatagac ctgagaaagt
    12661 tttagttagc cactccgtta gctgctccac cagttgagct actggtaatt ttgtttgtaa
    12721 tatcgtttcc aaaatgcatc actcctttta gttaaggtct tcgtctgaga tattagccaa
    12781 ggcttcaatc actcgtcctc gaataatgac acctagcaga cgattattat catctgtcac
    12841 tgcaatcgga gcagatgaat catagataag aggcaaaata tctgtaataa tagtatcttt
    12901 tgagacagtt ctcacatcgc gatcaatcac ttctgatagc ggtaaccctt ttttgcgcgc
    12961 ctctatagcg gcatcggccg tcaaactacc gactaattgg cggcggcgat tcgtcgccat
    13021 caacatagac acctcttcgt tgtgcatacg gttcaaggca acttgaggtc catctaattc
    13081 aacagtagtt gttaacggtt tgatcatgat attttgtgct gtcaagactt tagaacggtc
    13141 cacatcctca acgaattcac gcacaaagtc attggctggg ttagtcaaga tttcctcacc
    13201 agtaccaatt tgcataattt gtccgtcttt catcaaagca atccgatcac caatacgcaa
    13261 agcttcattc aggtcatgac tgataaagat gatggtttgt ttcatgctat cttgcaaatc
    13321 aagtaattca tcttgcatct cacgacgaat caaaggatca agcgctgaga atgcctcgtc
    13381 cattaagaga attttagggc tattagctag cgcacgggct aggccgacac gctgctgcat
    13441 cccaccagat agttggtttg ggtactggtc tttaaaatct aataggcctg aattatcaag
    13501 ggctttttct gccaatcgct ggcgttcttc tttgggaacg ccacgtaatt ccaaaccaaa
    13561 ttcggtattt ttcaaaatgg ttttatgagg aaagagggca aagctttgga aaaccatgtt
    13621 aatgtcatgg cggcgcacct cacgcaactg atctgctgac atggttgaga tgtctttacc
    13681 ttccagcaag atagatcctg ctgaaggttc aatcaaacga tttagcatgc ggacaagggt
    13741 tgatttccca cttcctgata gccccataat aacaaagatt tcaccttttt tgacctcaaa
    13801 actagcgtca tagacaccta cagtagcgcc tgttttctta aaaatctcac tcttattttt
    13861 gccagtcttt accatctcaa gagctgcttt ttgttttttg ccaaaaattt tactgagatg
    13921 tttgacttct aaaattgttt ccattcgttt tttctcctag gtaatcgttt atcatgtgaa
    13981 atagtgacat agctttggcg aaatattagc caatatttaa caaagtagct ctacacaatt
    14041 aataaacgtg tggtcacttt tttacttaaa aaaagaatct gccgaaacag aacataacta
    14101 tattatcacg tgtggtcact ttttgtcaag gacaataact cgatctgata ataccacaat
    14161 gataggtgat cgctttcatt cttttaggta tggtctttta accagtacaa ttttttatca
    14221 caactgatga ctatttcttt gctgattagg ctattgttac attagaaatc cttcttttac
    14281 ctgtaatatc tcttttgcgg ccatcgtaaa taacgaccac ggtgactctt aaacatttgc
    14341 tcatctctcc aaaaaaactc acctaagaca gtgtcctatg aagcgagtta gcttaatcag
    14401 gcactatcat cttaggtgag tagggtgttg cttatagtaa agctttaaat tggatattag
    14461 aatcttctaa gaagcagtca tcaaatccac gaggatgatg gtattcgata cgattttggt
    14521 cttgtggata ggtgtacttg ccaccaacct cccagataaa gggatggaaa tcatattgga
    14581 gacgttcttt gcgcattttc cacagttcaa ggatttcatc tgttgatgcc ataaagtttg
    14641 accaaatgtc ataatgaact gggataacga ctttagcacg gagattttct gccatacgaa
    14701 gaaggtcaac agaagtcatc ttgtcttgaa tcccaattgg gttttcccca tagttattta
    14761 acacaacgtc aatatcataa tcacgtccat gtttagcaaa gtagttcgaa aaatgagaat
    14821 ccgcaccgtg atagatggta ccacctggtg tttcaaaaat atagttaaca gctttgcgag
    14881 ccatgtcatc atctgtgata gctaaacctg ctaaatcacc atcttgagca tcagctcctt
    14941 gtattgggag agttaccaga caggtgcggt caaacgactc tacggcggtg atcttaatat
    15001 ctttgaattc aaagctatca cctggcttta agatcataat acgatctttt ggcacacccc
    15061 aatttttcca aacttcacca cattcatagg gacctacaaa tttaacgtgg tcaagttttg
    15121 ggttattaat aatggctgcc gcagtattga tatcgatgtg gtcactatga taatgagaaa
    15181 ccaaataata atccaactca ttaatcataa atggatcaat caccattggt tgggccctaa
    15241 gattgggctg taatttacga gctcctgcca tattagccat ttgatgtcca cgaaccatat
    15301 ccttaacttg cttggtcgct ttcccacgat ttgaccataa gtccatgaca acattagcac
    15361 cgcctggtgt cttaatccag ataccacagt ttcctaacca ccacatcgca aagttgtcag
    15421 ctggcacgac ttcttgctcg atttcttcat ttaaccatgt tccccattct ggaaacgtgt
    15481 taaggatcca tgattcacgt gtaatatctt gtactttagt cattagggtt tcctccgaat
    15541 ttgttcttcg tttctatact ttaagtatag actttcttgt caatgataaa aagaccaagt
    15601 gtcacacaga tctgtgttca aaagtggact gactttttca ttagctcagt ctaatactgt
    15661 ctcattttgc acactgtatc tcgcatcaat actgtattca aagctatatt aaaaacctca
    15721 gttatatttt tctatgtcat agaaaactga ggtactttga cgacttaaca ctcactcata
    15781 gcagctcgcg atgaatgagt ttttcaattt tagaaaccaa ggcataacga tctgattcat
    15841 cttttacaac agcttcaata gcttttgtga gctctgtaga aaagcgggaa tgctcactca
    15901 agcgtccttg ctttgaaaag cgtatcaatt tgatgatatc atcgtcactt aaaatagggt
    15961 taacaataag catcgggatt ttagtcttta gcgtatctgt agtagcaaca atcatatcga
    16021 ctgctaaaag atcgtaaaca ctctgatact gttctgtcgt aaaaacagct tcaatctgac
    16081 catttgctag atacctttga cactgtttga acaataactt ttgaatgcct atcccatcat
    16141 ctgaaacaat aacgagtttg gtcttttctt gctctgtatt attgtgacgc agctctcccc
    16201 cgagatggat agtcaagtag gcaatatcat cgtctgtcaa gctaatcgtc caatcttgtt
    16261 ccaaaatagt ggcacaagat tgagtcatag caaacaattc ttcgtatttt tctttaatat
    16321 ggtctgttaa gggattaact aaaaaaatcc cataagcttt acggtaaacg agcgccttac
    16381 agtgggtcgt caactgcttc agcaaatcct gtttatgcgt gaaatgtaac tgataacggc
    16441 tctccaactg gtcaataaag tgactaatgg ttgctcgcat gtcatcataa tcttgacttt
    16501 ccacgtgatg atcttgatct tttcgaaaag acaacatcaa catcgctacc atactaactt
    16561 cgatatcatc caaatgaagc ttaaaattat ggtaaagctc tctagctaag tcctgagcaa
    16621 tgtgatattc tttacgtttc caaatcaaat gaaattcttg tttaagagca gactttgttt
    16681 ctgaatcaag acgcatatta cgataactta aaagcatgaa aggtaaaatc tgcaacatga
    16741 attggctatc ttgggtatta attgtctttc ctaaactagc ttgagataaa aaaagatact
    16801 catgaaaata agtcaaaata tctttagaaa aataaaccga taagccttgt atctctgaga
    16861 gtttacggtt gaatatatca ataaagctag tattgccacc ttggtaaacg tccactaaca
    16921 atttgtacag gtattgaata agagccattg gatgacaacc aaaataatat cccctagcct
    16981 ttgtcgcatg aagctgaatc ttgtattgtt tgtcttccag ttcttccctt aactcagtca
    17041 ggtcatttaa aatcgtgtta cgagacacat cattgatctg catcagttta tcaatggtca
    17101 cgcgctcagt ggaaaccgca atataaatcg aggataggcg tctgcgctca tcacttttca
    17161 tgacataatt ataatcagtc acttcttcta ataataagcg acaagcagct ttttgatcag
    17221 ctgttaaaag aatgccaagt ctaggataag agataatctg atctactccc tttggcaaag
    17281 ccttattaat tttgtcaagc tggtaataaa ctttacgacg tgactgattt aaagcatgag
    17341 aaatggccat aactgtctca ggtgtttcta gtttaagtaa ataagacagc aagtcataac
    17401 tctttttatc taaaatcatc atgattacaa acctcctctc tgaaaatcca tccttgctta
    17461 ttttatcatg atttcagtga ctgcgcagcc aagttttgaa aaactcacca taataagatt
    17521 cgcttaatat gattcgagct aatcacctca actgatccaa caccaacacc ttatatctgg
    17581 tctctaagaa ctccacaaca tacgattcat caagtttgtg ctcattggcg tttaaacagt
    17641 tcatcgaaaa ggacaagtca aaaggacttg cccaaacaat gattaacaaa accttgaact
    17701 ttctggcttc tagtgagaac ctgtgctaat aagccatatc gtgacctcat ttttgaccgt
    17761 aataagcatt tgggccatgt ttacgcaaat aatgcttatc cataatgtat cgtggcgctg
    17821 gctctacgcg tggattgatt tgttctgtca agcgattcat tttagccact tcttctaaga
    17881 caaccgagtg gtaaacggct tgctctgggg ttttccccca cgtaaatggg ccatgattgc
    17941 gaaccacgat tcctggaacc gccataggat ctaagcctcg cttgctaaac tcttctaaaa
    18001 tcacgttgcc agtttcttgc tcgtaagccc catcaacttc tgcttttgtg agagaacgag
    18061 cacacggaac tggtccatag aagtagtcag catgcgtagt cccataaaac ggaatatcac
    18121 gacctgcctg agcccaacca acagcttctg tggagtgagt gtggacaata ccgcccactt
    18181 ctggccaagc tttataaagt tccacatggg ttggcagatc tgaagaaggg ttcaaatcac
    18241 cttcgaccac attcccatca aggtctgtaa caaccatgtt ttctggtgtc aagaggtcgt
    18301 agtccacccc agaaggcttg atgacaatac gtcctaattc acgacaaact tcagagacat
    18361 tgccccacgt aaatttgaca agaccgtgtt gtggtagaga cttgttagcc gcacagacac
    18421 gttcacgcat ttcttgcaag tttttcgcca tattatgcca accccgcttt ctcaataagt
    18481 gggtaaagga agtcctgagc ttctttgata gcagcttttg tttcctccac cgtgtcacaa
    18541 ttttctgacc acatttcaat caaaaaaggc ccattgtagt tggttttctt gaggaccgca
    18601 aacagctctt gccagtccac acaaccttga ccaaatggca catcacggaa ctgccctttt
    18661 gaggtttccg tgacagcata agtatctttc aagtggagag ctgcaatgga cttgtgacca
    18721 ttgtaaaatt cactccacaa atcattatgc caggcagaaa cattgcctgc atcaggataa
    18781 acaaagagat aaggcgagtc aatctctttt tcaactgcca agtatttttc aatagagttg
    18841 ataaaagggt catccataat ctcaatagaa agcatcactt gggcttcttc ggcccagtca
    18901 caagattgtc tcaagttttt gataaaacga gcacgggttt caggcgattt ttcttcatag
    18961 taaacgtcat aaccagctaa ttgaatggta cgaacgccta agtcctgtgc caattcaata
    19021 cattgtttca tcagtttaag agacttggct tcagtagcag gatcatttga gcctagtggg
    19081 taacgtctat gaccagaaaa acaaatggtc gggatgcgaa tgcctgtttc ataaatggct
    19141 ttcactaaat caagccgttc ttccttagtc cactctaagc gcgctaagcg agcatctgat
    19201 tcatcgacag acatctccac aaaatcaaaa cctaagtctt ttgcaaattg caagcgttca
    19261 cgccaggtaa actgttttgg cgttgctttt tcataaatac caataggacg cgccataaag
    19321 ttacccccag atacgtttga tttcatcttt gaaggcacgc gccgctgcag caggatcttc
    19381 tgcttcagta atgcctcgcc ctgcgataaa ggtaaagaca tcaacccctt caaaaagttt
    19441 caaagtgtct acatcaagcc caccagttac tgagacacga aagcccatgt caatcaattt
    19501 tttcaccttg ttaaggtctt tttcacccca agtttctcca gcaagaaggg catcacgaga
    19561 ttggtggtaa atcgcttggg aaatcccagc gtcaagccat aattgagctt gctcataagt
    19621 ccaatcacca taaagttcga tttgaatttc cccacggtca ccgcgctctt ctttaatagc
    19681 cttgagggct gcttccatgg ttggaatcgt cgcacagcaa atgcaggtca tccagtcagc
    19741 accacgtttt gcattgtttt tagcgaccgt accgccagcg tcagcacatt tggtatctgc
    19801 tacgataatt ttctctggga agaggctgcg taatacctca actagttcgc ttcccacttg
    19861 aagcaaacaa acggtaccag cttcaatcac atctacttcg tgacccacag caacggctgc
    19921 tttaacagcc ccctgtaagt cagaatggtc taaagcaact tgtaaatttg gaatatgtgt
    19981 catctctttt tcctaaaact cttagccacc gtgcaaactg gttaagagtt cttatccttt
    20041 caataatgat tgtgagtcgt tttatccgtt taagtcagta agctatcgat taagcgtcta
    20101 aatccaaacc ttctaaatac gggctatttt ttgattcttc taccatttct agaacctctt
    20161 taggactttg gcaggccacc aagcgttcaa tggcgttatc tagttcaaac aaggccacaa
    20221 tttgtgggat agccactgtt gtgtgaatag aagggtctgt agcggccaac gtcaaaagaa
    20281 cagaaacctc tttaccatct gagaaggtga ctggttttgt tagtgtaatc aaagcaaatg
    20341 cattgcgatt aacgcctaaa cctgcttctg catggggcat agccatacct ggcatcaaca
    20401 cataataagg gccgtatttt tctgttgatg caatgatggc gtcgtagtaa gctgacgtca
    20461 ctgctttgct atcaatcaaa ggttgaacag ctaattgaac agcctcttgc caagtatcag
    20521 cacttagccc caatcggatg gagttgttgt caataaaggc ttgttttaaa ttcataaaaa
    20581 ctatctcttt ctagtgtctt gctttgcaac acacaccatt tttactagtt ttatcctact
    20641 aaagagaaag agctgaaaac cttcgtcaca actccttcgt tttgattcag aattagctta
    20701 tagcacttct tgtagtttgg ttttaatttc attatcatcc atcaggttat caagaccaac
    20761 caagtgacct ttggtacgac catcaagctc atgaatcaag tggtttgatg ccactacaat
    20821 atcataacca gaagctagac ctttagcttc accaactgag caagaagctg actgaatatc
    20881 tgtcacacca agttgacgaa gcgcattttc aactttcatt ttgatcacca tagatgaccc
    20941 cataccattg ccacatgctg ttaatacttt aaccattgtt ttttcctccg aaaatgtttt
    21001 attattattt tgttagaata gcagagctat ttcttaagat gcagtgttat tgagcttccc
    21061 cacggtaata agcttctttg tccttagctt ttgcgaattg caattgtgga atggcaagta
    21121 agaagatgca aacaagcaca taccctgcaa taccaaggaa tttgaagagg tacccaaatg
    21181 gtaaccatgg aagaaccaag tcgatattac cgtgataacc acctgtaagt cctaaaagac
    21241 ctacagcaac agcaccaagt gcaacttgca aaatacctga gataaatgat agagctaccg
    21301 ctgctttcca gccaccacgt ttatctgcat aaaccgcaat tgccgcatta tcaaagaata
    21361 cgggaacgaa tcctgtgata attaagattg gatttttaaa gataacaaga agagcaatgg
    21421 tgattaattg accaatcaaa ccaaaggcaa atcctgaaag caccgcattt gaagaaccaa
    21481 agccatagga agctgcaacg tcaaccgctg ggaatgaccc tggaagcaat ttgcttgaga
    21541 tcccttgaaa ggcattggtc aactcagaaa cgaacatacg tacaccttgc atcaaaatga
    21601 agaggtaaac agagaaggtg agtgatgttt gaagaatata catgaagaaa gcttgcttag
    21661 ctgggttgaa agcgcctggt ccgattaact ctacattaga catgatgtct ggaccaagga
    21721 cagccaagat agctccaaag aagactaaca ttaaggttgc tgacgcaaca acggtatcgt
    21781 ggaaaatatt taagaaggtt ggtaatttta agttatcaag gttttcttct tttttaccaa
    21841 agattggagc aaccttatcc acaaaccaaa tcgcaaattg ttgttggtga ccaatcgcaa
    21901 agccaccgcc acctgttagg cgttgtgttg cttcaaccgt catgtttgag ctgactgccc
    21961 agtaaagacc acagatgata ccaaccgccc aagcaccaaa agcattttgg aattgaggga
    22021 ttaaaagaag gacaaagacc gaaatcgtcg ctgcctgttg gaccatgatg tgacctgtga
    22081 tgaaaagggt acgaacttta gtgaccttgc gaagtgcaac caaaagaatg ttgaccccaa
    22141 atccaatcaa gagagctgtc gtagcaacac tgatgaagcc catctcttct aacttagtat
    22201 tagcagcagc caaaccaaaa taaggatcaa taacggcggc tttcaattca aatttcttgg
    22261 caagagcaac caagatcgga cgaaaagtcg ttacaagacc gcctgctcca acgttcaaaa
    22321 tcaagtaacc aacggtagcc tttacaaaac cagcaaagac ctcataaatg ggttttttaa
    22381 ggagtaaata cccgattaaa accaaaagac ctacaaagaa cgctgggttt tgcaaaatat
    22441 tttgcgagaa ccagtttagg ggagctagga gcatttccat aagaactacc tcttttcaaa
    22501 gttaataaag ataaagtgtt tatatgttca ttataggtaa tgaaagcgtt ttaaaaaaga
    22561 ccaactatca cacaaagaag tgttcaatat tagacttaag ttaacttatt ttaaaattta
    22621 actacactta ataataagga ggaaataccc ctaaacaacg attaaaaacc acaagaacct
    22681 tcgttcttgt ggtttttcta gataagttgc tttttgaata gatcagctct ttggcattaa
    22741 ctgtgacgtg acgcaaataa ccactgaagg gctgtgtcac ctcaaactat ttgataacaa
    22801 tattaacgag tttgtttggt actgcgatga ctttgatgat gtctttacct gcgatttcgg
    22861 cttggacttt ttcgttagca agggcaactt cttgcaattc ttcgcgtgaa aggtctttag
    22921 caactacaag tttagctttg accttacctt tgatttggac aacgatttcc acatcgtttt
    22981 caaccaattt gctttcatca taacttggcc atggcacgta agagattgac tcacctgagg
    23041 ctgtcaaggc ttgccagagt tcctcaccta agtgcggtgc aaatggtgca atcaattgaa
    23101 caaagccttt ggcatagtcg ctaaagagtt tgtcttcctt gttggctgca ttgacaaaga
    23161 ccatgagttg agcgatagct gtgttgaatt tcatttggtc aacttgctca gtaacagcct
    23221 taacagtttc gttgtaaacc ttgtcaaggg ctccgctatt ttcctcagtg atctctttag
    23281 ttgtgatcaa acggtaaaca cggtccaaga acttacgtga gccttcaaga ccttcttcag
    23341 accaagcgat ggaagcgtca agtggtccca tgaacatctc gtagacacga agagtgtcag
    23401 caccgtactg ctcaacaaca tcatctgggt tgacaacgtt cttaagtgat ttagacatct
    23461 tagctggtgc ttgttcaagc tcttcaccag tttccacatg gaagaagcta ccatcacgtt
    23521 tttcaacctt gtcagtcgct acaagcgctc cccggctgtc gcggtagctt gttcccaaaa
    23581 tcatcccttg gttaaagagt ttttggaatg gctctttggt tggtacgaca ccgagatcgt
    23641 aaagaacctt gtgccagaag cgagcgtaga gaaggtgaag cacggcatgc tctgcaccac
    23701 cgacatagat atcaactggc aaccattgtt tgaggagttc ctcgtcagcc agtttttcgg
    23761 tgttatgtgg atcgatgtaa cgaagataat accagcttga acctgcccat tgtggcatgg
    23821 tgttagtctc acgacgacct ttaacaccat cttcacgagt cacttccaac cagtctgtca
    23881 cgttagcaag tgggctctca ccagtacctg aaggacgaat atccttagtt actggaagaa
    23941 ccagtggcaa ctcactctca ggaaccgctg ttgaggtccc atcttcccag tggatgattg
    24001 ggattggctc accccagtaa cgttgacggc taaagagcca gtcacgaaga cggtaagtga
    24061 ctttctcatt accaacacct tctgcttcaa gccattcgac cattttagca atagctgacg
    24121 ctttgtccag accatctagg aagtctgagt tgatgtgaag accgtcttct gtaaaggcag
    24181 cctcttcaac attaccacct tcaagaacag ggatgatgtc taggttaaat tgttttgcga
    24241 attcccagtc acgttcatcg tgggctggaa cagccatgat agcaccagta ccgtagctgg
    24301 caaggacata gtccgcaatc caaactggca tttcgttacc attaactggg ttaatcgcat
    24361 agctacccgt ccaaacacca gttttttctt tggcaaggtc tgtacgagca aggtctgatt
    24421 tgagacttgc ttgacgtttg tagtcagcca cggcttctgc ctggtcagct gttgtaatag
    24481 catcaaccaa ggcatgctca ggagcaagaa ccgcgtaagt tgcaccaaaa agggtgtcag
    24541 gacgtgttgt aaagacagtg aagtccttat ctgtatcctt aaccttgaag gttacattag
    24601 caccagttga tttatcaatc cagttgcgtt gcatgtcctt gattgattct ggccagtcaa
    24661 cttcttccaa gtcttctagc aagcgctctg cataggcagt gattttgagc atccattggc
    24721 gcatgggttt tctgacaact gggtagccgc cacgttctga tgtcccatca gggagaacct
    24781 cttcgttggc gatagctgtt cccaattctt caacccagtt aacgggaact tcggcttcgt
    24841 aggctagacc tttttcataa agcttggtaa agatccactg tgtccacttg tagtagttag
    24901 ggtcagttgt attaacttca cgatcccagt cgtatgagaa accaagagca ttaatctggc
    24961 gtttgaagtt agcgatgttt tcagctgtaa actcggctgg gtcattacca gtatccatag
    25021 cgtactgctc agcaggaagt ccaaaggcat cccaacccat tgggtggagg acattgtgac
    25081 cttgggcacg tttgaaacgg ctgagaatat cagttgctgt atagccttct gggtgaccaa
    25141 cgtggagacc agctccagat gggtatggga acatatctag ggcataaaac ttaggttttg
    25201 atgcgtctgt tcctgtttta aaggtgtgat tatctgccca aaaggcttgc catttgggtt
    25261 caatagcggt gtgatcgtaa aatgtcatgt gaaatctccc atgtaaaaaa tctagtcttc
    25321 ttattatagc atgagatgaa gcttttagga atggtcaaga catgactagc accttggctt
    25381 ttgagacttc agacgattag ctatttttaa accctgcttt agcatcaacc atcctcacaa
    25441 aaagctaata aaaaaagcca gattggctct gtaaacagag ccaatctgga caaatagact
    25501 taaaaagcaa gtgcttgctc aaaatcggcc aataaatctt cgatattttc aacaccacaa
    25561 gacaagcgca gcaaaccatc aacaacccct tgttccaacc gcgcttgtga cgataaacaa
    25621 gcatgagaca tcgttgctgg atgtgacaaa atggactcta ctccacctag actaacggct
    25681 aaaatcggca actgaatacg atgggtaaag gtcatcagct cctccttaga agccaactca
    25741 aaggataaaa cagcaccacc attcttagct tgttgcaggt gggtatcgtg attaggatga
    25801 ctgtctaagc cagggtaatg taccttaaga actttggggt gcttcgctaa ataattagcg
    25861 agctgctgag cattttttac cgcttgttcc atacggatcc ccatagtttt catgccacga
    25921 agaatcagcc aagcgtcttc gaccccaaca attgctccaa agtttttttg aaaaagcttg
    25981 agctggttgt agatagcctc attattggta gcagctaaac cagctaccac atcactatga
    26041 ccattgataa atttggtcac actttctacc acaatatcca cacccatagc taagggttct
    26101 tgatacaaac ttgtcataaa ggtattatca gccacagtta gcacaccata agctttggcg
    26161 agttgcacaa gctctgaaat atcacaaatt ttcaagagcg gatttgacgg tgtctctaga
    26221 taaatcatac gcgtattagg tctgatactt gcttttactg ttgctaaatc agccatatct
    26281 acaaagcttg tttcaatctg ataattgggt aaaatagctg tcgcaaattg acaagtccct
    26341 ccatagacct ccaatgggaa aatcacatga tctccggctt ttaagagcat caaaacagtt
    26401 gaaatagcag ccataccaga tgcataagcc accgcataag tcgctttttc taaacaggct
    26461 aacccgtctt ctaaagcttc agtcgttggg tttgaaaagc gtgtgtacaa atgtttttga
    26521 ctaggacaat acagttctga attgtggaac gtcgaagtct gatagatggg aactgaagct
    26581 gctcctgtaa actcgtcaat aaccgtatag ccatgtaata aggtcgtatt ttctctcatt
    26641 tcgtgcacct ccgcaatagc gttaactcca atgaactctc ttttagacaa tggtcccacc
    26701 ccaatggttg cacaaaccaa aaagctcttc aaaaaagcgt gatctgctaa cctataagaa
    26761 aagtagtggg caccttttaa atgaataatg aatttttcca acaatatcat ttttagatcg
    26821 attacattta ttatacaacc tttagctaag tttgcaagcc cttttatcat ctttgaccaa
    26881 gctgccaaca cctctccagc aagttaaagt atggctacct ttggccccat cgtctcaaaa
    26941 gcttagctta tcacactgac agtcctacct tgcatcgtca cctacttaat agggcaatag
    27001 tgccatcaaa ctttcatcaa aaggtcacaa tttatttcat ttaatgagaa acagaacttg
    27061 ttttcaccat gcgccttata gtatactgct aaaatactat ttatttttag gagagaaata
    27121 tagtgaaaaa caaattattt ttagttgccc tcgcgaccat aactgtccta gggccctttt
    27181 tagcaacccc tcatcaccag accgtgcatg cggctgagca gacagtaact aaaaaaacag
    27241 ctactgaaac atgtacatta gatggaaaaa tatgctacgg ttatgaaata tcaatgaaga
    27301 ccacaccgat agcatggttc aatctgagtc tcaagagcaa ggtttcttca gttggctttg
    27361 gacacaaata acaacttggt tttcctcatt aatgagctaa aaccatctcg tggcggtttt
    27421 ataatcctag gtcgccacac tttttattca ggtaaataat aataaaaaca ttaaaataat
    27481 acttgattta ttaacaaaaa gttagtataa tataatcaca atacaaatta aaggagttat
    27541 aaatgaaaaa gaaattatct ttatttatga tcgcaactgc tgctgttgca agccttagcc
    27601 ttgcaacact aggaagccac caaactgttt cagcaaatga tgctagctat agtaacggtt
    27661 tctacggaca cactggtcct gacagctggt tactttacac agtatggtaa acctcatacc
    27721 gtaacataga agctatcttc ttcgtaagac tacggttatg aaaagcctaa agctgcataa
    27781 gcagccttag gctttttgat tggctaaaac gtttttgcca atgacccctc aaggtaaaaa
    27841 agaaaagaca ctccgcctat aaaaggagag aagcccaaaa cacaagtcgt aacagttgct
    27901 agctaaccac caccaaaaaa gctgtaacac ctcaaatata atgataacca gattaacatt
    27961 attaataatt aatgtgattt atataagata aagaaatgct ttagtattgg ctttctttgc
    28021 ctttttcggt caaacatata acacaaagat taaaatgtga tattgaatca ttaatagaat
    28081 atgagtataa tataggtata ctataatt
//
LOCUS       DAUELZ010000011        27413 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.011, whole genome shotgun sequence.
ACCESSION   DAUELZ010000011 DAUELZ010000000
VERSION     DAUELZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27413
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.011"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            440..799
                     /gene="rnpA"
                     /locus_tag="VHI09_000377"
     CDS             440..799
                     /gene="rnpA"
                     /locus_tag="VHI09_000377"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEQ6120281.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            765..1592
                     /locus_tag="VHI09_000378"
     CDS             765..1592
                     /locus_tag="VHI09_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ6120282.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQKKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKARRLRERRARKKAMKRK"
     gene            1604..2518
                     /locus_tag="VHI09_000379"
     CDS             1604..2518
                     /locus_tag="VHI09_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEQ6120283.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINQKNAPAVNSADVEPEEIKATQKLEAED
                     TKVVPLMPEESPAQTLSNLAETVTETKAQQPSIPVEESEVPQDAGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            2832..2966
                     /gene="rpmH"
                     /locus_tag="VHI09_000380"
     CDS             2832..2966
                     /gene="rpmH"
                     /locus_tag="VHI09_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEQ6120284.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            3241..3945
                     /locus_tag="VHI09_000381"
     CDS             3241..3945
                     /locus_tag="VHI09_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEQ6120285.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMTEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
     gene            3994..5313
                     /locus_tag="VHI09_000382"
     CDS             3994..5313
                     /locus_tag="VHI09_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ6120286.1"
                     /translation="MNMKKLASLAMLGASVLGLAACGGKSQKEADASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTEEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKTFIQFITDDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAVSNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            5416..6303
                     /locus_tag="VHI09_000383"
     CDS             5416..6303
                     /locus_tag="VHI09_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ6120287.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLINTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIVILLTTSVGQPIILYIAAMGNIDNSLMEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            6316..7146
                     /locus_tag="VHI09_000384"
     CDS             6316..7146
                     /locus_tag="VHI09_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ6120288.1"
                     /translation="MTKKKLTASDILTAVMLCVLTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            7166..7621
                     /locus_tag="VHI09_000385"
     CDS             7166..7621
                     /locus_tag="VHI09_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEQ6120289.1"
                     /translation="MFYDRLKNISRYRGIHPHLDAAITFLQTTDLRQLAEGKYPILGE
                     KVFAVIQRNQLSKADNSLLEYHKRYADCHLLLAGNECIRYGIGNQAEVVPFEQEADIG
                     FVACDRTYDLDLVDDSFAYFFPNEPHQPNNFNQAGEAVTKCLIKVLMAD"
     gene            7665..8327
                     /locus_tag="VHI09_000386"
     CDS             7665..8327
                     /locus_tag="VHI09_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEQ6120290.1"
                     /translation="MTSKKQGLLHSLFNLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMAFYASLLDWREGQVSQLVTTYRSHFKYYFKSGLRLGLIELGIMSICLLDLF
                     LIRNQSGLVFQGFKVLCVAVLFLVVILFLYAYPQAVKRDLSLSTLFKRSFLLAGLFFP
                     WSFAFLTFICLTIFSLQLSLLTLFGGVSLLAIIGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            8339..9253
                     /locus_tag="VHI09_000387"
     CDS             8339..9253
                     /locus_tag="VHI09_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEQ6120291.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGNISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDRIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIADKDLEKAKALQYTINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            9275..10213
                     /locus_tag="VHI09_000388"
     CDS             9275..10213
                     /locus_tag="VHI09_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ6120292.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMAQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITLEKIENNLASS"
     gene            complement(10324..11154)
                     /locus_tag="VHI09_000389"
     CDS             complement(10324..11154)
                     /locus_tag="VHI09_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEQ6120293.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDSSERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
                     "
     gene            11439..12230
                     /locus_tag="VHI09_000390"
     CDS             11439..12230
                     /locus_tag="VHI09_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEQ6120294.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALTLANKYANIYATIGWHPTEAGSYSEAVEEAIVSQLSHPKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKATTANAKRVFKLD"
     gene            12202..12792
                     /gene="rnmV"
                     /locus_tag="VHI09_000391"
     CDS             12202..12792
                     /gene="rnmV"
                     /locus_tag="VHI09_000391"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEQ6120295.1"
                     /translation="MQRGYSSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHARFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            12906..13778
                     /gene="rsmA"
                     /locus_tag="VHI09_000392"
     CDS             12906..13778
                     /gene="rsmA"
                     /locus_tag="VHI09_000392"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEQ6120296.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVED
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            14204..15076
                     /gene="rsgA"
                     /locus_tag="VHI09_000393"
     CDS             14204..15076
                     /gene="rsgA"
                     /locus_tag="VHI09_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEQ6120297.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            15086..15748
                     /locus_tag="VHI09_000394"
     CDS             15086..15748
                     /locus_tag="VHI09_000394"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEQ6120298.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGADIMTIHTESTRHIHGAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            15741..16373
                     /locus_tag="VHI09_000395"
     CDS             15741..16373
                     /locus_tag="VHI09_000395"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEQ6120299.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVPQKAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMLTYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            16375..17646
                     /locus_tag="VHI09_000396"
     CDS             16375..17646
                     /locus_tag="VHI09_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEQ6120300.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     MTYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEEINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLNASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQATKSLLNMPLLEEENNEN"
     gene            17636..18574
                     /locus_tag="VHI09_000397"
     CDS             17636..18574
                     /locus_tag="VHI09_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEQ6120301.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVEEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
     gene            18841..19680
                     /gene="purR"
                     /locus_tag="VHI09_000398"
     CDS             18841..19680
                     /gene="purR"
                     /locus_tag="VHI09_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEQ6120302.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEGNIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            19671..22292
                     /locus_tag="VHI09_000399"
     CDS             19671..22292
                     /locus_tag="VHI09_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEQ6120303.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIDAVEKTLSQQKAELTKLATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKA
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEALAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQETLAALQAKQSGLEATIATTEHQLTLLKTLANEKEYRHLDED
                     IATVPDLQVAPPLTGVKPLSYSKIDTTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESK"
     gene            22500..22913
                     /gene="rpsL"
                     /locus_tag="VHI09_000400"
     CDS             22500..22913
                     /gene="rpsL"
                     /locus_tag="VHI09_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEQ6120304.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            22934..23404
                     /gene="rpsG"
                     /locus_tag="VHI09_000401"
     CDS             22934..23404
                     /gene="rpsG"
                     /locus_tag="VHI09_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEQ6120305.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            23888..25966
                     /gene="fusA"
                     /locus_tag="VHI09_000402"
     CDS             23888..25966
                     /gene="fusA"
                     /locus_tag="VHI09_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEQ6120306.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            26307..27317
                     /gene="gap"
                     /locus_tag="VHI09_000403"
     CDS             26307..27317
                     /gene="gap"
                     /locus_tag="VHI09_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEQ6120307.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
BASE COUNT         8411 a         5107 c         5925 g         7970 t
ORIGIN      
        1 atttacgaag tcatgagttt atcgagcaat caatccccaa ttcaatcaaa atattgtttt
       61 ctctctctcc ataaacaaat agtatttttg atatgatggc tcggcagaca aaatgacgat
      121 gtcagttgtc tttgtttgga tatcacatta gctggttgta aaatcagtat taaagcccgg
      181 cttgccgggt tttttgatag gtaaatacag ggataagtag ctgacgtctt gtggattctg
      241 actttttatg gttgttttct gaaggttatt cctttgtcga aagaagaact atattacgaa
      301 aagatggagt gacaagaggc agttgaagca caaaaactag aaatagtaca gaaagaggct
      361 taatctgaaa gggttagggc tttttaattt tttaggggat gtggtataat ggtaataatt
      421 tagatattgg agttaaattt tgaagaagac ctatcgtgtc aagcgtgaga aagatttcca
      481 agccatattt aaggatggaa aaagtacagc aaatcgaaaa tttgtcattt atcatttaaa
      541 tagaggccaa gaccacttcc gtgtgggtat ttctgtcggt aaaaaaatag gaaatgcagt
      601 caccagaaat gcagtcaaac gaaagatacg tcatgttatc atggcattag ggcatcagct
      661 gaagtcagag gatttcgtgg tgattgcccg taagggtgtc gagtctttgg agtatcaaga
      721 gcttcaacaa aatttacatc atgttttaaa gttagcacaa ttgcttgaga aaggttttga
      781 gagtgaagaa aaacattaaa attgcaagaa tagtcccctt agttctatta ttagtggcct
      841 gtggtcgtgg tgaggtaacg gcacaatcat ctagtggttg ggaccagttg gtttacttat
      901 ttgccagagc aattcaatgg ctttcctttg atggttcaat tggtgttggc attattcttt
      961 ttacccttac tatccgtctc atgctcatgc ctttgtttaa tatgcaaatc aaatcaagcc
     1021 agaagatgca agacatccaa cctgagctta gagaattgca aaagaaatac gctggtaaag
     1081 acacacaaac gcggatgaag ttggctgaag aaagtcaagc cctttacaaa aaatatgggg
     1141 ttaaccctta tgctagcctc ttacccctct taattcagat gccagttatg attgccctat
     1201 tccaagcctt gacacgggta tctttcttaa aaacagggac tttcttgtgg gtggaattgg
     1261 cacaacatga tcatttgtac cttttaccag ttttggcagc tgtcttcact ttcttgtcca
     1321 cttggttgac caatctagcg gctaaagaaa aaaatgtcat gatgactgtt atgatttatg
     1381 tgatgccgct aatgatcttt ttcatgggct ttaacttggc tagtggagta gtgctctatt
     1441 ggacggtctc aaacgccttt caagtggtac aactactgct attaaacaat ccttttaaga
     1501 ttattgccga aagacagcgt cttgccaatg aagaaaaagc acgccgtctt cgtgaacgtc
     1561 gtgctcgtaa aaaagcaatg aagagaaaat aggagtttag gttatggtat tatttacagg
     1621 aaaaacagtc gaagaggcta tcgaaacagg acttcaagag ttagggttat cacgccttaa
     1681 agcgcatatc aaagtcattt caaaagagaa aaaaggtttc ctcgggttcg gtaaaaagcc
     1741 agcccaagtt gatatcgaag gtatcagcga caagacggtt tataaggctg ataaaaaagc
     1801 cactcgaggt gttccagagg atattaatca aaaaaatgct cccgcggtca attctgctga
     1861 tgtagaacct gaagaaataa aagccactca aaagttagaa gcagaagata ccaaagtggt
     1921 tcctctcatg cctgaagaaa gtccagcgca aaccctttct aatcttgctg agacagtcac
     1981 tgaaacaaaa gcacaacaac cgtcaatccc agtcgaggaa tcagaagtgc cccaagatgc
     2041 tggtaatgat ggtttcagca aagatattga aaaagctgct caggaagtgt ccgattatgt
     2101 gactaaaatc atctatgaaa tggacattga agctaccgtt gaaaccagca acaatcgtcg
     2161 ccaaatcaat ttgcaaatcg aaacgccaga ggcgggacgt gtgattggtt accatggtaa
     2221 ggtcttgaaa tcattgcagt tgttggcgca aaatttcttg cacgaccgct attcaaaaaa
     2281 tttttcagta tccttgaatg tccatgatta tgtggaacat cgaacagaaa ccttgattga
     2341 ctttacgcaa aaagttgcca aacgcgtctt ggaatctggt caagattaca ccatggatcc
     2401 catgagtaac agtgagcgta aaatcgttca taaaaccgtt tcatccattg aaggggttga
     2461 tagttattct gaaggcaatg accctaaccg ctatgtggta gtcagtcttc aacgttagat
     2521 cctaaaaaga atctttggta gttagtttag ctatcgaaga ttttttgtat ggccagctta
     2581 accgtttgtt ttactgatct cattgatcat tgttgctatt gataaggttt caacggtaag
     2641 gcaaaggaac atgtcaaaga agagacactt tatgaaatga gcggcctctt aaatgagaga
     2701 gaaactgttg aaaaacgaaa ataaatcttg acagtagacc ctctttaaga tagaatatta
     2761 tagtatgttt agtaactgat atcacaaata gccggctaaa cgaatacaaa atctatgagg
     2821 aggtattcac tgtgaaacgt acttatcaac caagtaaaat ccgtcgtcaa cgtaaacacg
     2881 gattccgtca ccgtatgtca actaaaaacg gacgtcgcgt gcttgctgca cgtcgtcgta
     2941 aaggacgtaa agtattatca gcttaattgc aaaaaataca actaaagtaa accaagagtg
     3001 ctcgaggctc ttggtttttt aacgttttct aatcggaaat ttggaatgta taagtatggt
     3061 gtcaactcct taccttctta tttgttgtat gaaagagcat tccaaataat agaaaataat
     3121 ataaaaaatg aaagtagatt ctaaaagtct cattgctatt gaaagcgctt tatttttttg
     3181 ttataataac tttgtgaacg ttatttttat taaaactaag aaacacaagg agtagaaact
     3241 atgcctgata aaccaaccaa agaaaagctc atggagcagt taaagggtgg gattatcgtt
     3301 tcttgtcagg ctttgccagg tgagcctctt tattcagaga ctgggggtat tatgccactg
     3361 ctagcaaaag ctgctcaaga agctggtgct gtcggcatca gagctaattc ggttagggat
     3421 attaaagaaa ttcaagccat aacagaccta ccaattatcg gtatcatcaa aaaagattac
     3481 ccgccgcaag agccttttat tacggcaacg atgaccgaag tggatcaatt agctgcctta
     3541 aatattgcag tgattgctat ggattgtacc aagcgtgacc gacatgatgg cttggatatt
     3601 gctagcttta ttcgacaagt taaggaaaag taccccaatc agcttttgat ggcagatatt
     3661 agtacctttg atgaaggact agtggcccat caggctggca ttgattttgt tggaaccacc
     3721 ttgtcaggct acaccccgta tagtcgtcag gaagcaggtc ctgatgtggc attgatagaa
     3781 gctctttgca aagcaggcat tgctgtcatt gccgaaggaa agattcattc cccagaagaa
     3841 gccaagaaaa ttaatgactt aggtgtggca ggtattgttg ttggaggggc catcacaagg
     3901 cctaaggaaa ttgcagagcg ttttatcgaa gcgctcaaat cctaatagtg gttgtcaaac
     3961 tagaaacaca catcaaaaaa aggagatttt acgatgaaca tgaagaagtt agcttcatta
     4021 gcgatgcttg gtgcatctgt tttaggacta gcagcttgtg gcggaaagag ccagaaagag
     4081 gctgatgcaa gtaaatctga tactgctaag acagaaatca catggtgggc tttcccggtc
     4141 ttcacgcaag aaaaagctga agatggtgtg ggaacctatg agaagaaatt gattgctgcc
     4201 tttgagaaag ctaatccaga aatcaaggta aaattggaaa ccattgattt tacatctggt
     4261 cctgagaaaa tcacaactgc tattgaagct ggaacagctc ctgatgtcct ctttgatgct
     4321 ccaggtcgta tcattcaata tggtaaaaat gggaaattgg ctgacttgaa tgacttgttc
     4381 acagaagagt ttaccaaaga tgttaacaat gacaaattga ttcaagcctc aaaagcagga
     4441 gatacagctt acatgtatcc tatcagctct gctccattct acatggcgtt gaataaaaaa
     4501 atgctcaaag atgctggcgt ccttgacctt gtgaaagagg gttggacaac agatgatttt
     4561 gaaaaagtct tgaaagcatt gaaggacaaa ggatataacc caggttcttt ctttgctaat
     4621 ggtcaaggtg gtgaccaagg accacgtgcc ttctttgcta acctttatag tagtcatatt
     4681 accgatgaca aagtaaccaa atacaccaca gatgatgcta actctattaa agccatgacc
     4741 aagatttcca attggattaa agatggttta atgatgaatg gctctcagta cgatggttca
     4801 gctgatattc aaaactttgc gaatggccaa acttccttta ccattctttg ggcgcctgca
     4861 caaccaggca tccaagctaa attgttagaa gctagtaaag tggattacct tgaaatccca
     4921 ttcccatcag atgatggcaa accagaacta gaatacctcg taaatggttt tgcggtcttt
     4981 aataacaaag atgaacaaaa agtcgctgcc tctaagacat ttatccaatt tatcactgat
     5041 gataaagaat ggggacctaa aaatgttgtt cgtacaggtg ctttccctgt aagaacttct
     5101 tatggggatc tttacaaaga caaacgaatg gaaaaaattg ctgaatggac aaaattctac
     5161 tcaccatact ataacacgat tgatgggttt gctgaaatga gaactctttg gttcccaatg
     5221 gttcaagccg tctctaatgg tgatgaaaag ccagaagatg ctttgaaagc cttcactgaa
     5281 aaagcaaaca agacaatcaa aaaaacacaa taagtgttcc taacaagtgg cttcttttca
     5341 atgcacatgt caaatggtca ctggaaagaa gcttttcttt ttagaaaagt tgaacagaaa
     5401 aaagaggtat ttcaagtgaa cgtcaataaa ttaaaaatgc gagagacgct catctcatac
     5461 gctttcttag ctccggtctt ggttttcttt gtgatttttg tcttgatacc gatgattatg
     5521 ggctttgtga cgagcttttt caattattcc atgacggatt ttacctttgt tggttttgct
     5581 aattatgcta ggatgtttca agatcccatt ttcatgaagt ctcttattaa caccttgatt
     5641 attgttattg gttcggtacc tgttgtagtt ttcttttccc tctttgtggc ggctaaaacc
     5701 tacgacaaaa atgtagtggc gcgttcgttt taccgggcag ttttctttct gcctgttgtt
     5761 accggaagtg ttgcagtgac ggtggtttgg aaatggatct atgatccgat gtcagggatt
     5821 ttgaattatg tcttaaaata tgctcatgtg attgaacaaa acattagttg gctgggagat
     5881 aagcactggg ctttgttagc tattattgtt attttattaa ccacatctgt tggacagcct
     5941 attattctat atattgctgc tatgggaaat attgataatt ctttgatgga agctgcgcgt
     6001 gtagacggcg ccacagaatt tcaggtgttt tggaatatca agtggccaag tctcctgcca
     6061 acaaccttat atattgctgt cataacaacc attaattcct tccaatgttt tgctttgatt
     6121 caacttttga cgtctggagg gccaaattat tcaaccagta ccctcatgta ctatttgtat
     6181 gaaaaggcct ttaagctctc tgaatacggc tatgccaaca caatgggtgt attcttggca
     6241 gtcatgattg caatcatcag ctttgctcaa tttaaaattt taggtaatga tgtggaatat
     6301 taggaggatg ttaagatgac gaaaaagaaa ctaaccgcat cagatatctt aaccgctgtg
     6361 atgttatgtg ttttgaccat tttgtttatt tttccatttt attggattat gacaggagca
     6421 tttaaggctc aagccgatac cattatgatt ccaccgcaat ggtggccaaa ggcacctact
     6481 attgaaaatt ttaaggcctt ggtagtgcaa aatccagcct taaaatggtt gtggaatagt
     6541 gtctttattt cggtggcgac catgttcttg gtttgtggca cttcctcttt ggctggctat
     6601 gctttggcca aaaagcggtt ttatggacag cggcttttgt tttcgatttt tattgctgct
     6661 atggccttgc caaaacaggt tgttctagtg cctcttgtgc ggattgttaa ttttatggga
     6721 atccatgaca ctttggcggc tgttattttg cctcttgtgg gctggccatt tggtgttttc
     6781 cttatgaaac agttctcaga aaatattcca accgagttat tggaatccgc caagattgat
     6841 ggatgtggcg aaattcgtac ctttttcaat gtggctttcc ctattgtcaa accaggcttt
     6901 gcagcgctag caatttttac ctttatcaat acttggaatg attattttat gcagttggtg
     6961 atgttaacct ctcgggaaaa tttaacgatt tcacttgggg ttgccactat gcaagctgaa
     7021 atggctacta attatggctt gattatggca ggggctgcca tggctgcagt gcctattgtg
     7081 acggtatttc ttgtcttcca aaaatcattt acccaaggca ttactatggg tgctgtgaaa
     7141 ggttaaatca agaaaggttt ctaacatgtt ttatgatcgt ttaaaaaata ttagccgtta
     7201 ccgagggatt caccctcatt tggatgcggc gattaccttt ttacaaacca cagatttgag
     7261 gcaactagct gaagggaaat atcctatttt aggggagaaa gtatttgcag ttattcaaag
     7321 aaatcaattg agtaaagcgg acaattcatt gttggaatac cacaaacggt atgcagattg
     7381 ccaccttttg ctagctggga atgagtgcat tcgttatggt ataggtaatc aagctgaagt
     7441 agtgcctttt gaacaagaag ctgatattgg ctttgtagcc tgtgatcgaa cctatgacct
     7501 agatttggtt gatgatagct ttgcttactt ttttcctaat gagcctcatc aacccaataa
     7561 ttttaaccaa gcaggggaag cagttacaaa atgcttaatc aaagtgttaa tggcagatta
     7621 ggttgatttg gcagtgaacc aattgatgga aaggaagtga tggtgtgacg tcgaaaaaac
     7681 agggactttt acattctctt tttaatcttg atagtaaatg gatgagggct agtgcagcac
     7741 tatttgattt gctcgtgttt aatctcttat ttgtcttgtc ctgccttcct cttctgacaa
     7801 ttggggttgc taaaatggct ttttatgcga gtttgttgga ttggcgagaa ggtcaagtta
     7861 gtcagcttgt tactacttat agatctcatt ttaaatatta ctttaaaagt gggctacgcc
     7921 ttggccttat tgagcttggg ataatgagta tttgtctctt agatcttttc ttaattcgaa
     7981 accaatcagg cctagttttt caagggttta aagtactttg cgttgctgtt ttatttctgg
     8041 tggttatact ctttttgtac gcttatcctc aggccgtcaa aagagacctt tccctatcta
     8101 cgctgtttaa gcgaagcttt ttactagcag gactcttttt tccatggagt tttgctttct
     8161 tgacttttat ttgtctcacg atatttagcc tacaattgtc tctgttaacg ttatttggag
     8221 gcgtgtcttt gctagctatc atcggcatca gtagcctaac ttacctctac ttgattatca
     8281 tggaatctct tcttaggcga ttcccactta ataatgacat tgaataaagg acattactat
     8341 gacagatctt acgaaatacc aaggcattat tcctgccttt tacgcttgtt acgatgacca
     8401 ggggaatatc agccctgagc gtgtgcgtgc cttgacccag tactatattg acaaaggggt
     8461 tcaggggctt tacattaatg gctcttctgg agagtgtatt taccagagcg tttttgaccg
     8521 tcaattggtg ttagaaaatg tcatggcagt cgctaagggc aagctgacga ttattaatca
     8581 cgttgcctgc aacaacacta aagatagtat tgaattggca gctcattcag aacgtttggg
     8641 cgtggatgcc attgcagcta ttccgcctat ttatttccgg ttaccagaat atgcagttgc
     8701 tgattattgg aatgcgattt cttcggctgc tcctcacaca gattttatca tctataatat
     8761 tcctcaacta gcaggggttg ccttgacccc aagcctttat aagactatgt tggctaataa
     8821 gcgcgtgatt ggcgttaaaa attcatctat gccagtgcag gatattcaaa ccttctgtgc
     8881 gattggtggg gacgaccgta ttgtttttaa tggcccagat gaacaatttc taggagggcg
     8941 cttgatgggt gctgctgcag gcattggtgg tacctacggg gctatgccag aattattttt
     9001 aaggctgaac caacttattg ctgacaagga tttagaaaag gccaaggccc ttcaatatac
     9061 tattaatgag attatcggtg tccttgtttc tgctcacggc aatatgtatg gggttattaa
     9121 ggaagtactt cgtattaatg agggtcttga tattggttct gttcgatcac ctttagctga
     9181 gttggtcgag gaagataggg ttatttgcca acgggctgct gctctgatta atcaggctaa
     9241 ggaaaccttt taataagatg cgaggggata acaaatgaag cattatttag caattgatat
     9301 tggtggtact gctatcaagt atggcttgat ttctgaaaca ggagacctgt tggaaaaaga
     9361 ggaaatggcc actgaggctt ataaaggtgg tccctctatt ttggaaaagg tcaagggctt
     9421 ggtaaagacc tatcaagacc aaatggatct ggctggagtg gccatttctt ctgcaggaat
     9481 ggttaatcct gataagggag aaatctttta tgccggccct cagattccta actatgctgg
     9541 aactcagttt aaaaaagaga ttgaagagac tttcgggtta ccgtgtgaag tggaaaacga
     9601 tgttaactgt gctggtctag ccgaagctat ttctggtagt gccaaggatt atcctgtggc
     9661 tctttgcttg acaattggga cgggaatcgg tggctgttta ttgtttaatt cccaagtttt
     9721 tcatggtagt agtcattcag cttgcgaagt tggttacctt catttgtcag atggtcagtt
     9781 tcaggatctg gcatctacca cagctcttgt tcaagaggtg gccttagctt atggcgatga
     9841 cattagccaa tgggatggtc gtcgtatttt tgagcaggct aaagcgggtg atgcgatttg
     9901 tatagcagcc attagtaagc aggtggatta cttaggacaa ggtattgcta atatctgcta
     9961 cgttgttaac ccaaatgtgg tggtgcttgg tggaggaatc atggcgcaaa aagactattt
    10021 agctgataaa ttaaagacag ctcttgatag ctacttggtt tctagtcttg ccaagaaaac
    10081 tcagctgaag tttgctagtc atggaaataa tgctggtata ttaggagctt actaccattt
    10141 taaacaaaag aatgagcgat cttgttcatt tataactctt gagaaaatcg aaaataattt
    10201 agcgagtagc taatcttagt ggctgtgagt ctatatgacg tgaagagttc ttgtttaagt
    10261 atactggggg aggtgaatga cgacccttta aaaagaaaac aacgcccttt ttgctattgt
    10321 agtctaaagg tcgttatttt ctttgtcaat aatagtgttt ttgaagattt tttctttgtg
    10381 cggtttgtca atcgctaaaa cgtaagcata aataatgtcc atcataataa gaagaggaaa
    10441 ttgtggtgac acacgattcc cataatgtag ttggtgggtt gatgatacag gaataatatc
    10501 taaggagtcg tcaaattcgg tttgattatc tgttgttaag aggacagttt tggcccctcg
    10561 ctgactagct tgatgaagtg ctgtgatgac agagtttgtt ttgccagata aagaaaaacc
    10621 aaaaacaagg caattttcat tgacgaggct atttgcccaa gtaaaaccat cagtgtctga
    10681 ataagcatca cagattaaac caagtcgcat aaagcgtagc ttcatttctc ttgctactag
    10741 tccagaagat cctttgccaa agaaatagac tcgctcagaa gaatcgatca gcttggctaa
    10801 gttcagtaat ttttcctcat taacgagctc ataggtttta ttaataagag catcgtagtc
    10861 catcagcacg cgctttgtca attctaagtg aatagattgg aaagtctctt gtgattcttg
    10921 aagactgtga aggtaatcaa aggcaaaagc acgataacca gtaaaaccac atttttttgc
    10981 aaatcgagtc aaggcggctt gagagatgtg tagtcgctta actatctcgg atgcggtcaa
    11041 ctcttgaggt gtcagatcgg ttgtaataaa aaaatgagca atcccttttt ctaaactggt
    11101 catatgttcc aatgatgctt cgattttggt aattaggcgg tggtttttag gcatagcaaa
    11161 gcacactcct tacagtttaa ctacctacta gtatagcata gatcgctaac agaaggtagc
    11221 gacccttttt gtgatacaga tttgttcaac ccactttatt tgagtaggtt gtagtatttt
    11281 tgagggctta tgtaggagcc gactattaaa ggtcatttgt caaaaaagta gaaaatagac
    11341 aagtgtcagc tagcaaaggg ttttacgatc aacctaaacg gcagaagttc tcaccaagtt
    11401 ttctgatgtg gttaagatct ttatgttata ataacatcat gacaaataaa gtagaaaaac
    11461 taactatttt tgatacccat acgcacctga atgtagcaga atttcaagga cacgaaactg
    11521 aagaattgac tttggctcaa gaaatgggcg tggcctacca taatgtggtt ggctttgacc
    11581 aggccactat tagtggggca ctaactctag ccaataaata tgccaatatt tatgcaacta
    11641 ttgggtggca tccaacagaa gcaggttctt attcagaggc tgttgaggag gctatcgtct
    11701 ctcaattatc acatcccaaa gtgattgcct taggtgaaat tggcttagac tattattgga
    11761 tggaggaccc caaagaggtg caaatagagg tttttaagcg ccaaatgcaa ttagcaaaag
    11821 accatgattt gccttttgtg gtgcacaccc gcgatgcctt ggaagatacc tatgaggtca
    11881 tcaaagcagc tggtgttggc ccccgtggag gcatcatgca ttcttactca gggtcattag
    11941 agatggctga acgtttcatt gaacttggca tgatgatttc cttttcaggt gtggtcactt
    12001 tcaaaaaggc tcttgatatt caagaggcag cgcagcactt gcctttggat aagattttgg
    12061 tggaaacaga tgccccttat cttacaccag ttccaaaacg tggcaaacaa aatcatacag
    12121 cttatacccg ttatgtggtt gataaaatcg cagagctacg ggggatgact gtggaagaag
    12181 ttgcaaaagc aacgactgct aatgcaaaga gggtattcaa gcttgactga aaaaattaat
    12241 attcaagagg ttcttgttgt agaaggcaag gacgacacgg ctaacctacg ccgtttttac
    12301 gaggtggata cctatgagac tagaggctct gctatcactg aagaagattt agaacgaatc
    12361 aatcgcctaa acgatttgcg tggtgttatc gttttaacag acccagatta taatggtgag
    12421 cgcattcgta agctcattat ggctgctgtg ccaacagctc gtcatgcctt tttaaaccga
    12481 aacgaggcgg ttccgagttc taagagtaag ggtcgttcct taggggttga acatgccaga
    12541 tttgaggatc tccaaaaggc acttgctcat gtcactcagc aatatgatga tgagtcttat
    12601 tttgacattc gtcaaacaga cctgattcgt ctagggcttt taatggcgtc agatagccgt
    12661 aaacgcaggg aatacttggg tgaaaagctc cgcatcggtt atgccaacgg caaacaactg
    12721 ctcaagcgcc tcgaattatt tggcattacc ctagcagaag tggaagaagt gatggagaca
    12781 tatgaggagt aaagagtaca ataaaatata acagtcccat taactgaatg gcgtgtccta
    12841 gcaaaaaagt ttaggctagt ggttcaaatg ccaaaaagaa agtgatcata aaaagaagag
    12901 aatatatgag aattgcagat tatagcgtga ccaaggctgt ccttgatcgt cacggattta
    12961 cctttaagaa atcttttggt cagaactttt tgaccgatac caatatttta cagaaaatcg
    13021 ttgacacggc tgagattgat cagaatgtca atgttatcga gattggtcct ggaattgggg
    13081 ctttgacgga atttttggca gaaaatgctg ctgaagtgat ggcttttgag attgatgacc
    13141 gcttggtgcc gattttggca gataccttgc gcgattttga taatgtgcaa gtggtcaatc
    13201 aagatatttt gaaagctgat ttgcagaccc aaatcaagca gtttaaaaat cctgatttgc
    13261 ctatcaaggt ggtggctaac ctaccttact acatcacaac gcctattctc atgcacttga
    13321 ttgagagcaa aattccattc caagagtttg tagtcatgat gcagcgtgaa gtggcagatc
    13381 gtatttctgc ggagccaaac actaaagctt atggttcctt gtccattgcg gtacagtatt
    13441 acatgactgc caaggttgcc ttcattgtgc cgcgtaccgt ctttgtacct gcaccaaatg
    13501 tcgatagtgc tatcctcaaa atggtgcgtc gcgaccagcc tttgattgag gttgaggacg
    13561 aagatttctt tttcagagtg tctcgtctca gttttgttca tcgtcgcaag accctgtgga
    13621 ataacttgac tagccatttt ggcaagtctg aagacatcaa ggctaaactt gaaaaaggtc
    13681 tggcactagc agatatcaag ccttctattc gtggtgaggc cttgtcgatt caagattttg
    13741 gcaaattagc tgatgcttta aaagaagttg gattataaaa gctttccccc ggtgacaata
    13801 aaaaaattcc cttgtagaca aaacagagat gttcagttca gggggatttt tcttattgaa
    13861 gctggccgtt aaggcatcgt agtgcagagc tagttttttt aatacctcgg aaaaaaaggg
    13921 gcaattaaca agagcgtgac tatagcggta cggttagact ctttttcgta aatagcaaag
    13981 gtcttcttaa aattcatatc gtttcctacc tcaaaaaatc atcagtgatc tgatttttag
    14041 ttgataaaat attctatttt taaaaagcag tgacctaaat gatagccagt ggtctagaga
    14101 atcttgacag ttttttaagg cgagtgaaac ttagactcaa atgctgattt gggagcggtt
    14161 ttctgatata atagagttat tatagtaggg caaaggagca cagttgcaag gtaaaatcat
    14221 taaatctctg gctggttttt attacgtgga gtcagaagga caggtatatc agacgcgagc
    14281 gcgtggtaat tttaggaagc gcggtgagac accttatgtt ggagatatcg tggatttctc
    14341 agcagaggac aactctgaag gttatatttt agcgattcat cctcgtaaaa acagtttggt
    14401 aagacctcct atcgtcaata ttgaccaagc agtggttatt atgtctgcta aagaaccaga
    14461 atttaacagc aatcttctag accgctttct catcttgctt gaacacaaag ccattcatcc
    14521 agtggtttat atctctaaga tggacttatt agatagtccc gaggagatta aggctattgg
    14581 tcgacagtat caggcaatcg gttacgattt tgtgaccagt cttgaggaat tgttgccttt
    14641 gctagctgat aaaattaccg tctttatggg acaaacaggt gttgggaaat caacccttct
    14701 caaccgaatc gcccccgaat tggctcttga aacaggagaa atctcagata gtctagggcg
    14761 tggtcgtcac accacccgag ctgtgagttt ttacaatacc catggcggaa aaatcgcgga
    14821 tacgccaggt ttctcatcat tagactatga tattgctaat gctgaagatt tgaacgaggc
    14881 ttttccagag ctgcgccggc tgagccatga gtgtaagttt agatcctgta cccataccca
    14941 cgaacccaag tgtgctgtca aggcagcgct tgaaacggga gagttgtggc cagttcgtta
    15001 tgagcattac ctgcaatttc tcagtgaaat tgaaaaccgt cgtgaaacat ataaaaaagt
    15061 tatcaaaaga aagtaggtct actccatgtc aactctaaaa attgccccat ctattctagc
    15121 agctgattac gctaattttg cttctgagct agctcgtatt gaagaaacag atgccgaata
    15181 cgttcacatt gacattatgg atggtcaatt tgttcctaac atcagttttg gtgcagatgt
    15241 tgtggcaagt atgagaaagc acagtaaatt ggtttttgat tgtcatttaa tggtggttga
    15301 tcctgaacgc tatgttgagg cttttgcaca agctggtgct gatatcatga ccattcacac
    15361 agaaagtact cgtcatattc atggagccct tcaaaaaatc aaggcagctg gcatgaaagc
    15421 aggggtagtg attaatccag gaactcctgc aacagcccta gaacctttgc tagacttagt
    15481 tgaccaagtc ctcattatga cagtcaatcc aggttttggg ggtcaggcct ttatcccaga
    15541 atgtttagaa aaagtggcta ccgtagccaa atggcgagat gaaaaaggat tgtcctttga
    15601 cattgaggtt gatggcggag tagacaataa gaccattcgt gcttgttatg aagcaggggc
    15661 caatgtcttt gtggcaggtt cttacctttt caaggccagt gacctagtga gccaagtcca
    15721 aaccttaagg acggccttga atgtctaaag ttgctctttt tgcgggagga gatctctctt
    15781 atatcagccg tgattttgat tattttgtcg gtattgatag agggagccta tttttattag
    15841 aaaatgggct tcctttgaat atggctgttg gtgattttga ttcggttccc caaaaagcgt
    15901 ttacggatat aaaagagaag gcagagcttt ttattactgc tcatccagaa aaaaatgata
    15961 cggatacgga attagccctt aaagaggtct ttgctcgttt tcctgaggcg gaagtgacca
    16021 tttttggggc ttttgggggt cgaatggacc atctcttatc caatattttt ttgcctagcg
    16081 accctggtat tgctcccttt atggcacaaa tcgccttgcg tgaccaacaa aatatgctga
    16141 cctaccgtcc ggcaggtcag caccttatcc atcaagaaga agggatgacc tatgtggcat
    16201 ttatggcaga aggagaagct gacttgacca ttactggtgc taaatttgag ttgacgcaag
    16261 ataatttttt caaaaagaaa atctattcca gtaatgcctt tatccatcaa cctattacag
    16321 ttagtttgcc aagtggctac ttgattatta ttcaaagtaa agattggagt tagtatggac
    16381 cttatcttgt tccttttggt cttggttctc ttaggtttag gggcttatct gttgttcaaa
    16441 gtcaacggcc ttcaacatca gcttgcccaa accctagaag gcaacgcgga taatttgtct
    16501 gaccaaatga cctaccagtt ggatacagct aacaagcaac aattgttaga gctaacacag
    16561 ctgatgaacc gacaacaagc agacctttac caacaattaa cagatattcg tgacgtcttg
    16621 caccgtagtt tgtctgatag tagagaccgg tctgacaaac gcttagaaga aattaaccag
    16681 caggtcaacc aatcgctcaa aaatatgcaa gaatctaacg aaaaacgttt ggaggaaatg
    16741 cgccagatcg ttgaagaaaa attggaggaa accttaaaaa atcgtctgaa cgcctctttc
    16801 gattctgtat ccaagcaact agaaagtgtc aataaaggct tgggagaaat gcgtagcgtg
    16861 gctcaagatg tgggtacttt aaataaggtt ttgtccaata ccaaaacacg aggcatttta
    16921 ggcgaacttc aactaggcca aatcattgag gatatcatga catcaagcca gtacgaaaga
    16981 gaatttgtaa cggttagtgg ttctagtgaa cgcgtagaat atgcgattaa gctcccagga
    17041 aatggtcaag gcggctatat ttacctaccg attgactcaa aattccctct tgaagattat
    17101 taccgattag aagatgctta cgaagttggt gataaactgg ccatcgaggc tagccgaaaa
    17161 gcacttctgg cagctatcaa acgctttgcc aaagacattc ataaaaagta cttgaacccc
    17221 ccagagacga ccaatttcgg agttatgttc ttaccaacag aaggtcttta ttcagaagtg
    17281 gtcagaaatg cggctttctt tgatagcctt cgtcgggaag aaaatattgt ggttgcaggt
    17341 ccttcgaccc tgtctgcttt gctcaattcc ttatctgttg gtttcaagac ccttaatatc
    17401 caaaaaaatg ctgatgacat cagtaaaatt ttgggcaatg tcaagttaga attcgataaa
    17461 tttggcggcc tgcttgccaa ggctcaaaag caaatgaata cagctaataa tacgctggat
    17521 cagctcattt caacaaggac aaatgccatt gttcgagcct tgaataccgt tgaaacttat
    17581 caagaccaag caacaaaatc tctcttgaac atgcccttat tagaagagga aaataatgaa
    17641 aattaatcaa atgaaaaaag accagctttt tgaagggttc tacctcatta agtcggcaga
    17701 ggttcgtaag acaagggctg gtaaagattt tattagcctt acctttcagg atgacactgg
    17761 cgagatttca ggaaatctgt gggatgccca accttataac gttgaagaat ttactgctgg
    17821 taaagtcgtg tttatgaaag ggcgacgcga ggtgtataat ggtacccctc aggttaatca
    17881 aatcaccttg cgaaatgttc gcccagggga gcccaatgat ccaaaggact tcaaagaaaa
    17941 ggcacctgtc agtgttgaag aagtacgtga ctatttggaa cagatgctct ttaaaattga
    18001 aaatgcaacg tggcaacgaa ttgtaagagc tctttatcgt aagtatgata aagaatttta
    18061 tacttatcct gcagctaaaa caaaccacca tgcttttgag tcaggcctcg cctaccatac
    18121 tgctacgatg gtcagactag cagatagcat tggagatatc taccctgact taaataagag
    18181 tctactgttt gcaggtatta tgttacatga cttagctaaa gtcatagaat taacaggacc
    18241 agataataca gaatacaccg ttcgtggtaa cttgattggg catatttctc tcattaacga
    18301 agaaattaca aaagtaattt cagaactaca aatcgatgat accaaagaag aagtcattgt
    18361 ccttaggcac gtgattttaa gccatcacgg gcaattagaa tacggtagtc cggtacgtcc
    18421 acgcatcatg gaagctgaaa ttattcatat gattgacaat attgatgcta atatgatgat
    18481 gatgacaaca gctcttagcc gtgttagcga gggcgagatg accaatcgga tttttgccat
    18541 ggataatcgg tctttttaca agccaaacta ttgatcagtg tttctcaagt aatagttcct
    18601 ttgcttttgt gatgattttt gatgtgctta acacttatga tggtttcata agtgtttttg
    18661 gctcttgtta tggttattaa gtagaatgtc ctttcagtag gaatgttgat tagtttatct
    18721 tagattgaat tcttgcatga tattaataag aacaaaaaaa ccaatatttt cctaatcgaa
    18781 tgttcgtttt ttattaactt tatggtataa tagcaaaaag tacaaaaaaa tggggaaact
    18841 atgaaattaa gacgaagtga acgtatggtt gtcatttcaa attatttgat taacaatccg
    18901 tataaattaa ctagtttaaa cacctttgca actaaatatg aggccgcaaa atcttctatc
    18961 tcagaagata tcgctattat caagaaagct tttgaagaag gtaacattgg tgatatcgat
    19021 acacttacag gagcaagtgg aggcgtaatt tttaccccca gtatttcaga aacagaagcg
    19081 cgtaccattg ttgaagacct gtgtcaaagg ttatcagaga gtgatcgcat cttacctgga
    19141 ggctatatct atttgtcaga cttgctgagt acacctaaaa ttttacaaaa tattggtcgt
    19201 attattgcca atgcctttaa aggagaaaag attgacgctg tgatgacagt tgcaaccaaa
    19261 ggagttcctt tagcaaatgc agtggctaat attttaagtg ttccttttgt cattgttaga
    19321 cgtgatttaa aaataacaga aggatcgaca gtgtctgtca attatgcgag tgcttctagt
    19381 gatcgtattg aaaagatgtt tctgtcaaag cgtagtttga aacccaatag tcgtgtattg
    19441 attgtagatg atttcttgaa gggcgggggg acgattacag gaatgatcag tctcttgaca
    19501 gaatttgaca gcactttagt tggtgttgct gtgtttgcag aaaacgctca atcagagcgt
    19561 gagcaaatga ccttcaaatc attgctgaaa gtttcagaga ttgatgtcaa aaacaacaat
    19621 gttgttgtag aagttggtaa tattttcgat aaatggaaaa aggagagaga atggacttag
    19681 agcaaacgaa gccaaaccaa gttaagcaga aaattgcttt aacctcaaca attgctttat
    19741 tgagtgccag tgtaggcgta tctcaccaag tcaaagcaga tgatagagcc tcaggagaaa
    19801 cgaaggcgag taatactcac gacgatagtt taccaaaacc agaaacaatt caagaggcaa
    19861 aggcaactat tgatgcagtt gaaaaaactc tcagtcaaca aaaagcagaa ctgacaaagc
    19921 ttgctaccgc tctgacaaaa actactgctg aaatcaacca cttaaaagag cagcaagata
    19981 atgaacaaaa agctttaacc tctgcacaag aaatttacac taatactctt gcaagtagtg
    20041 aggagacgct attagcccaa ggagccgaac atcaaagaga gttaacagct actgaaacag
    20101 agcttcataa tgctcaagca gatcaacatt caaaagagac tgcattgtca gaacaaaaag
    20161 ctagcatttc agcagaaact actcgagctc aagatttagt ggaacaagtc aaaacgtctg
    20221 aacaaaatat tgctaagctc aatgctatga ttagcaatcc tgatgctatc actaaagcag
    20281 ctcaaacggc taatgataat acaaaagcat taagctcaga attggagaag gctaaagctg
    20341 acttagaaaa tcaaaaagct aaagttaaaa agcaattgac tgaagagttg gcagctcaga
    20401 aagctgctct agcagaaaaa gaggcagaac ttagtcgtct taaatcctca gctccgtcta
    20461 ctcaagatag cattgtgggt aataatacca tgaaagcacc gcaaggctat cctcttgaag
    20521 aacttaaaaa attagaagct agtggttata ttggatcagc tagttacaat aattattaca
    20581 aagagcatgc agatcaaatt attgccaaag ctagtccagg taatcaatta aatcaatacc
    20641 aagatattcc agcagatcgt aatcgctttg ttgatcccga taatttgaca ccagaagtgc
    20701 aaaatgagct agcgcagttt gcagctcaca tgattaatag tgtaagaaga caattaggtc
    20761 taccaccagt tactgttaca gcaggatcac aagaatttgc aagattactt agtaccagct
    20821 ataagaaaac tcatggtaat acaagaccat catttgtcta cggacagcca ggggtatcag
    20881 ggcattatgg tgttgggcct catgataaaa ctattattga agactctgcc ggagcgtcag
    20941 ggctcattcg aaatgatgat aacatgtacg agaatatcgg tgcttttaac gatgtgcata
    21001 ctgtgaatgg tattaaacgt ggtatttatg acagtatcaa gtatatgctc tttacagatc
    21061 atttacacgg aaatacatac ggccatgcta ttaacttttt acgtgtagat aaacgtaacc
    21121 ctaatgcgcc tgtttacctt ggattttcaa ccagcaatgt aggatctttg aatgaacact
    21181 ttgtaatgtt tccagagtct aacattgcta accatcaacg ctttaataag acccctataa
    21241 aagccgttgg aagtacaaaa gattatgccc aaagagtagg cactgtatct gatactattg
    21301 cagcgatcaa aggaaaagta agctcattag aaaatcgttt gtcggctatt catcaagaag
    21361 ctgatattat ggcagcccaa gctaaagtaa gtcaacttca aggtaaatta gcaagcacac
    21421 ttaagcagtc agacagctta aacctccaag tgagacaatt aaatgatact aaaggttctt
    21481 tgagaacaga attactagca gctaaagcaa aacaagcaca actcgaagct actcgtgatc
    21541 aatcattggc taagctagca tcgttgaaag ccgcactgca ccagacagaa gccttagcag
    21601 agcaagccgc agccagagtg acagcactgg tggctaaaaa agctcatttg caatatctaa
    21661 gggactttaa attgaatcct aaccgccttc aagtgatacg tgagcgcatt gataatacta
    21721 agcaagattt ggctaaaact acctcatctt tgttaaatgc acaagaaact ttagcagcct
    21781 tacaagctaa acaaagcggt ctagaagcta ctattgctac cacagaacac cagttgactt
    21841 tgcttaaaac cttagctaat gaaaaggaat atcgccattt agacgaagat atagctactg
    21901 tgcctgattt gcaagtagct ccacctctta cgggcgtaaa accgctatca tatagtaaga
    21961 tagatactac tccgcttgtt caagaaatgg ttaaagaaac gaaacaacta ttagaagctt
    22021 cagcaagatt agctgctgaa aatacaagtc ttgtagcaga agcgcttgtt ggccaaacct
    22081 ctgaaatggt agcaagtaat gccattgtgt ctaaaatcac atcttcgatt actcagccct
    22141 catctaagac atcttatggc tcaggatctt ctacaacgag caatctcatt tctgatgttg
    22201 atgaaagtac tcaaagagct cttaaagcag gagtcgtcat gttggcagct gtcggcctca
    22261 caggatttag gttccgtaag gaatctaagt gatgcattcg tcataagcaa ttgcatcaaa
    22321 ggaaaaattc tatttgacat gaagtaccgt ctgtgttatt atattagacg gtacttttta
    22381 cttttggtct ctcaaaagtg tacagagacg tgctgacaat tgttgcaaaa agtgcaccgg
    22441 atatgggctg tcaccaagtg ctatatcaac caaaaataaa aaaacacagg agaatgtaga
    22501 tgcctacaat taaccagttg gtacgtaaac cacgtaaatc taaaattgaa aaatcagatt
    22561 caccagcttt gaacattggt tacaacagtc acaaaaaagt tcaaactaaa atggctgccc
    22621 cacaaaaacg tggcgttgct actcgtgttg gaacaatgac acctaaaaaa cctaactcag
    22681 cccttcgtaa attcgctcgt gtacgtttga gcaaccttat tgaagttaca gcttacatcc
    22741 caggtatcgg acacaacttg caagagcaca gtgttgttct tattcgtggc ggacgtgtaa
    22801 aagaccttcc aggggtacgt taccatatcg ttcgtggcgc acttgatact gcaggtgttg
    22861 ctgaccgtaa acaaggccgt tctaaatacg gtgcgaaacg tccaaaagga taatagaagg
    22921 gggataagag aaaatgagtc gtaaaaatca agcgcctaaa cgcgaagtat taccagatcc
    22981 attatataac tcaaaaatcg taacacgtct tatcaaccgt gttatgcttg acggtaaacg
    23041 tggtacagct gctactatcg tttacgatgc tttcagcgct atcaaagaag caacaggaaa
    23101 tgacgctctt gaagtatttg aaacagctat ggacaacatc atgcctgtac ttgaagtacg
    23161 cgcacgccgt gtcggtggtt ctaactacca agtcccagtt gaagttcgtc cagaacgtcg
    23221 tacaacactt ggacttcgtt ggttggtaaa cgcatcacga gctcgtggtg aacacactat
    23281 gaaagatcgt cttgctaaag aaatcatgga tgctgcaaac aacacaggtg catcagttaa
    23341 gaaacgtgaa gacactcaca aaatggctga agctaaccgt gcctttgctc acttccgttg
    23401 gtaatataag atattttagc gtcaaacaaa tgctagccaa aatagggaaa ttgacgcaga
    23461 aacttcagtt tctaggtaat tttatctttg tggcagacat ttgtagctaa aattcaacta
    23521 atataagatg tgagagtgtt aagcaagtcc aagacaaaat aggaatagtg acgcagaaac
    23581 ttcagtttct aggaactatt atctttttgt cagtggcttg tagctcgaac tcaattaata
    23641 agatacgaag gcgtatagaa cgcaaagtga aaataggaaa tttgacgaag aaacctttgt
    23701 ctctaggaaa gtttatcttt ttcacacagc gtttagcccg agttcaatga ataagatacc
    23761 aagccttata aaggcaatag gtattatctt taccaacaaa tatttagata acgggtaggt
    23821 cctgcctatc cgtttttgct aaatagtgct ataatgaaag agtaaactaa aaaaatagga
    23881 gaaacacatg gctcgcgaat tttcacttgc aaaaactcgt aatatcggta tcatggctca
    23941 cgtcgatgct ggtaaaacaa ctacaacaga gcgtatcctt tactacactg gtaaaattca
    24001 taaaatcggt gaaacacacg aaggcgcatc acaaatggac tggatggagc aagagcaaga
    24061 acgtggtatc actattactt ctgccgctac aacagctcaa tgggatggtc accgcgttaa
    24121 catcatcgac acaccaggac acgtggactt caccatcgaa gtacaacgtt ctctccgtgt
    24181 tcttgatggt gctgtaaccg ttcttgactc acaatcaggt gttgagcctc aaactgaaac
    24241 agtttggcgt caagcaactg aatacggtgt tccacgtatc gttttcgcca acaaaatgga
    24301 taaaatcggt gctgacttcc tttactcagt acaaaccctt catgaccgtc ttcaagcaaa
    24361 tgcacaccca atccaattgc caatcggtgc agaagatgat ttccgtggta tcattgactt
    24421 gatcaagatg aaagcagaaa tctataccaa cgaccttggt acagatattc ttgaagaaga
    24481 tattccagaa gaatacttag aacaagctca agaataccgt gaaaaattaa tcgaagcagt
    24541 tgctgaaact gatgaagact tgatgatgaa ataccttgaa ggtgaagaaa tcactaacga
    24601 cgaattgatt gctggtatcc gtaaagcaac aatcaacgtt gaattcttcc cagtactttg
    24661 tggttctgcc ttcaaaaaca aaggtgttca attgatgctt gatgcggtta tcgcatacct
    24721 tccaagccca cttgacatcc ctgcaatcaa aggtgttaac ccggatacag atgctgaaga
    24781 agaacgtcca gcatctgatg aagagccatt tgcagctctt gccttcaaga tcatgactga
    24841 cccattcgta ggtcgtttga cattcttccg tgtttactca ggtgttctta atagcggttc
    24901 ttacgttatg aacacatcaa aaggtaaacg tgagcgtatc ggacgtatcc ttcaaatgca
    24961 cgcaaacagc cgtcaagaaa ttgaaacagt ttacgctggt gacatcgctg ccgctgttgg
    25021 tttgaaagac acaacaactg gtgactcatt gactgatgaa aaagcaaaag ttattcttga
    25081 gtcaatcgaa gttccagaac cagttatcca gttgatggtt gagccaaaat ctaaagcaga
    25141 ccaagacaag atgggtgttg cccttcaaaa acttgctgaa gaagatccaa cattccgcgt
    25201 tgaaacaaac gttgaaactg gtgaaacagt tatcgctggt atgggtgaac ttcaccttga
    25261 tgtccttgtt gaccgtatga aacgtgagtt caaagttgaa gctaacgttg gtgctcctca
    25321 agtatcttac cgtgaaacat tccgtgcttc tacacaagct cgtggattct tcaaacgcca
    25381 atctggtggt aaaggtcaat tcggtgatgt ttggattgaa tttacaccaa atgaagaagg
    25441 taaaggtttc gaattcgaaa atgctatcgt tggtggtgtg gttcctcgtg aatttatccc
    25501 agctgttgaa aaagggttga tcgaatctat ggcaaacggt gttcttgcag gttacccaat
    25561 ggttgatgtt aaagctaagc tttacgatgg ttcataccac gatgtcgact catctgaaac
    25621 tgccttcaag atagctgcat cacttgcact taaagaagca gctaaatctg cacaaccagc
    25681 tatccttgag ccgatgatgc ttgtaacgat tacagctcca gaagataatc ttggagacgt
    25741 tatgggacac gtgacagctc gtcgtggacg cgttgatggt atggaagcac atggtaacag
    25801 ccaaatcgtt cgtgcttatg taccacttgc tgaaatgttc ggttatgcaa cagttcttcg
    25861 ttctgcaact caaggacgtg gtacgttcat gatggtattt gaccactatg aagatgttcc
    25921 taagtcagtt caagaagaaa tcattaagaa aaacaaaggt gaataaccac cactaagagg
    25981 ttgaggataa ctatcctaac ctctttttta gtgtaacaga acgttatagt acagtttgaa
    26041 gttttggaaa tctttcccaa taaaaagata aattacagaa tagaaaaaag aaagcccttt
    26101 cctatatttg gtgggttttg aaggctagaa gaaagaagct gccctttatg aaaatggcta
    26161 atttatttgc attttttctg aaatagttat ataatagttc tgttgaaagg ttgttgcaga
    26221 tgactgtaag ttaatctttt cacaataggt agggagcatt ccctctaaat aatattcttt
    26281 tgattttcat aaggaggaaa tcactaatgg tagttaaagt tggtattaac ggtttcggtc
    26341 gtatcggacg tcttgcattc cgccgtattc aaaacatcga aggtgttgaa gtaactcgta
    26401 tcaatgacct tacagatcca aatatgcttg cacacttgtt gaaatacgat acaactcaag
    26461 gtcgttttga tggaacagtt gaagttaaag aaggtggatt tgaagtaaac ggaaacttca
    26521 tcaaagtttc tgctgaacgt gatccagaaa acatcgactg ggcaactgat ggggttgaaa
    26581 tcgttcttga agcaactggt ttctttgcta aaaaagaagc agctgaaaaa cacttacatg
    26641 ctaacggtgc taaaaaagtt gttatcacag ctcctggtgg aaacgatgtt aaaacagttg
    26701 ttttcaacac taaccacgac attcttgacg gtactgaaac agttatctca ggtgcttcat
    26761 gtactacaaa ctgtttagct cctatggcta aagctcttca cgatgcattc ggtattcaaa
    26821 aaggtcttat gactacaatc cacgcttaca ctggtgacca aatgatcctt gacggaccac
    26881 accgtggtgg tgaccttcgt cgtgcacgcg ctggtgctgc aaacatcgtt cctaactcaa
    26941 ctggtgctgc taaagctatc ggtcttgtta tcccagaact taacggtaaa cttgacggtg
    27001 ctgcacaacg tgttcctgtt ccaactggat cagtaactga gttggttgta actcttgaca
    27061 aaaacgtttc tgttgacgaa atcaacgctg ctatgaaagc tgcttcaaac gatagcttcg
    27121 gttacactga agatccaatc gtttcttcag atatcgtagg cgtatcatac ggttcattgt
    27181 ttgacgcaac tcaaactaaa gtaatggaag ttgacggatc acaattggtt aaagttgtat
    27241 catggtatga caacgaaatg tcttacactg ctcaacttgt acgtactctt gagtacttcg
    27301 caaaaattgc taaataatta gttataacga aagagagctt ggtttatgct gagttctttt
    27361 tcgtatactt taaacactac cttaaatgta tgattttaac gttttgaact att
//
LOCUS       DAUELZ010000012        26067 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.012, whole genome shotgun sequence.
ACCESSION   DAUELZ010000012 DAUELZ010000000
VERSION     DAUELZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26067
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.012"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..251
                     /gene="gpsB"
                     /locus_tag="VHI09_000404"
     CDS             <1..251
                     /gene="gpsB"
                     /locus_tag="VHI09_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEQ6120308.1"
                     /translation="EVDEFLDNVIKDYENFNAQIEALKAENEALKKAKFQARNTVSAT
                     VQQPVPQPTRVAQSATNFDILKRISKLEKEVFGKQIIE"
     gene            265..633
                     /gene="rnpB"
                     /locus_tag="VHI09_000405"
     ncRNA           265..633
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHI09_000405"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            696..1850
                     /locus_tag="VHI09_000406"
     CDS             696..1850
                     /locus_tag="VHI09_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEQ6120309.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWENYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKVNGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            1864..3333
                     /locus_tag="VHI09_000407"
     CDS             1864..3333
                     /locus_tag="VHI09_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120310.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERVIPEPQITKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDVVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQEAQDQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGNQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(3478..3960)
                     /locus_tag="VHI09_000408"
     CDS             complement(3478..3960)
                     /locus_tag="VHI09_000408"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEQ6120311.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHNLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(4034..5365)
                     /locus_tag="VHI09_000409"
     CDS             complement(4034..5365)
                     /locus_tag="VHI09_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEQ6120312.1"
                     /translation="MEIIGSLGVLVGVIVIIYLSVKEVNIIIAAPLATSLVILFNQMD
                     PTTTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGATTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVTFGLFYMKYCLAKSMARG
                     ETYATYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     AILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIAPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIVIAFDLFS"
     gene            complement(5436..6215)
                     /locus_tag="VHI09_000410"
     CDS             complement(5436..6215)
                     /locus_tag="VHI09_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEQ6120313.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETLLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(6248..6907)
                     /locus_tag="VHI09_000411"
     CDS             complement(6248..6907)
                     /locus_tag="VHI09_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ6120314.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPKGVTITLQSENGFVGLTGLTDDHYDPTIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(6909..7559)
                     /locus_tag="VHI09_000412"
     CDS             complement(6909..7559)
                     /locus_tag="VHI09_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ6120315.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDALVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITIDGKDYLLETPLKADVALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREIVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            complement(7583..8770)
                     /locus_tag="VHI09_000413"
     CDS             complement(7583..8770)
                     /locus_tag="VHI09_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ6120316.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQIAIHSGIPSDKTAFTLDMVCGSGLKAIQLATQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGAGPIPATQKVLKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            8969..9865
                     /locus_tag="VHI09_000414"
     CDS             8969..9865
                     /locus_tag="VHI09_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ6120317.1"
                     /translation="MDIKQIRYFIAIVENHFNLSQAAELLYVSQPTLSMMINDFEKRE
                     NVKLFKRKRGRIIGLTYLGDNYYKDAQKVLSLYDDMFLKLHDHSKGLKGSINIGIPPL
                     ILSVVFSEVMPKLILENPGIQFNVKEIGAYQLKNELLVGNVDVAVLLSPTGIADNLVE
                     TYETQRSELSVCLSPRHRLASKKVIQWEDLTDEQLALFDPSFMVHHLVLEACERHQVR
                     PNIILTSSSWDFMLNSTKINHNVLTICPKPITELYQLKDIKCIPMERPISWRVVLTRL
                     RKKSNSEIEAYIMDDLLQSFFK"
     gene            9995..11602
                     /locus_tag="VHI09_000415"
     CDS             9995..11602
                     /locus_tag="VHI09_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEQ6120318.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            11712..12347
                     /gene="gmk"
                     /locus_tag="VHI09_000416"
     CDS             11712..12347
                     /gene="gmk"
                     /locus_tag="VHI09_000416"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEQ6120319.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNPFKAK"
     gene            12363..12680
                     /locus_tag="VHI09_000417"
     CDS             12363..12680
                     /locus_tag="VHI09_000417"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEQ6120320.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            12745..15129
                     /locus_tag="VHI09_000418"
     CDS             12745..15129
                     /locus_tag="VHI09_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEQ6120321.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKSDRDFLFEGKPSVLYSTLDREKGKIALKGIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVTYFVTNHLIRIDERPIDRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKVKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKQQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSNNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            15191..16126
                     /locus_tag="VHI09_000419"
     CDS             15191..16126
                     /locus_tag="VHI09_000419"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEQ6120322.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTLLLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSN
                     QEVFSHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGA
                     LSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR"
     gene            16116..17438
                     /gene="rsmB"
                     /locus_tag="VHI09_000420"
     CDS             16116..17438
                     /gene="rsmB"
                     /locus_tag="VHI09_000420"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEQ6120323.1"
                     /translation="MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSSKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLIYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSDRAVAIMESLFMRSKASTRVTDPLKLEEVAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            17476..18216
                     /locus_tag="VHI09_000421"
     CDS             17476..18216
                     /locus_tag="VHI09_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEQ6120324.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            18213..20111
                     /gene="pknB"
                     /locus_tag="VHI09_000422"
     CDS             18213..20111
                     /gene="pknB"
                     /locus_tag="VHI09_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEQ6120325.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIIFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSP
                     SETTSQTP"
     gene            20234..20926
                     /locus_tag="VHI09_000423"
     CDS             20234..20926
                     /locus_tag="VHI09_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEQ6120326.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            20923..21927
                     /locus_tag="VHI09_000424"
     CDS             20923..21927
                     /locus_tag="VHI09_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ6120327.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITINYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLHMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGNDDE
                     "
     gene            21920..22561
                     /locus_tag="VHI09_000425"
     CDS             21920..22561
                     /locus_tag="VHI09_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ6120328.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            22598..23998
                     /locus_tag="VHI09_000426"
     CDS             22598..23998
                     /locus_tag="VHI09_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ6120329.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPRLQEKADVITKKVEEDGILYALEELGLIDKELQFPQL
                     DLENHTGPKVTIKTNHGDMTLVLFPEHSPKTVANFLGLAKEGYYDGIIFHRVIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPTPIAAAYAENGGTPHLDRRHTVFGQLADDASFKVLDLIASVETGAQ
                     DKPKEDVIIKTIEVFD"
     gene            23998..24375
                     /locus_tag="VHI09_000427"
     CDS             23998..24375
                     /locus_tag="VHI09_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ6120330.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALDNGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKASLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(24393..25334)
                     /gene="cysK"
                     /locus_tag="VHI09_000428"
     CDS             complement(24393..25334)
                     /gene="cysK"
                     /locus_tag="VHI09_000428"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEQ6120331.1"
                     /translation="MTKIYKTITELVGQTPIIKLNRLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKASPETAIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIKTK"
     gene            complement(25462..>26067)
                     /locus_tag="VHI09_000429"
     CDS             complement(25462..>26067)
                     /locus_tag="VHI09_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEQ6120332.1"
                     /translation="SGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKKEHYKANHSC
                     FAMIIGNNSQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFGGIKLGTGGL
                     IRAYSNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTETETKFSDTI
                     KTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
BASE COUNT         8314 a         4692 c         5375 g         7686 t
ORIGIN      
        1 aagaagttga tgaatttctt gataatgtaa ttaaagatta tgaaaatttc aatgctcaaa
       61 ttgaagcttt aaaagctgag aacgaagcct taaaaaaggc gaagtttcaa gctagaaata
      121 cagtttcagc gactgttcaa caaccagtac cgcaaccgac tcgtgttgct caatcagcta
      181 ctaattttga catcttgaaa cgtattagta aattggaaaa agaggttttt ggtaaacaaa
      241 ttattgagta atgaaaactg tgcaattttt ggataatcgc gtagtatttt aatactatga
      301 ggaaagtcca tgctagcact ggctgtgatg ccagtagtga ttgtgctagg cgaacacata
      361 agcctaggga tgtgcataca cattacggcg aaggaaatgg ctaagtcgtt tgatatgcca
      421 aagtacttct gaaagtgcca cagtgacgta gtttttatgg aaacgtaaaa aatggaacgc
      481 ggtaaacccc tcaagctagc aacccaaact ttggtagggg catgggatag ttggaaacga
      541 acaagctatc ctgactgtca acagacggta gacagatgat tatcgaagga aataattcct
      601 agttatttcc ggaacaaaac atggcttata gaaaattgca taataggtta agctagcact
      661 tgctagcttt tatttgattt aaaagaaaga gatatatgaa agaaacattt agattagtcg
      721 caactgcagc cgcaggttta gaagctgtcg ttggtaaaga agtcagagca ctaggttttg
      781 attgtcaggt tgaaaatggg aaagtttatt ttgaaggtga tgtagaagcg attgttaaaa
      841 caaatctttg gttacgtgca gcagatcgta tcaaaattat cgtaggacaa tttcctgcta
      901 gaacctttga agaattattc caaggtgtct ttgccttgga ttgggaaaat tatttaccgc
      961 taggagctaa atttccaatc tcaaaagcca aatgtgttaa gtctaaattg cataacgagc
     1021 caagtgttca ggcgattacc aaaaaagctg ttgttaagaa actgcaaaaa catttccaca
     1081 ggccagaagg agttcctctt caagaagttg ggtcaacgtt taacattgag gtatctattt
     1141 taaaagacca agccactatt atgattgaca cgacaggatc tagcctattt aaacgagggt
     1201 accgcgttca aaagggtggg gctcctatta aagaaaatat ggcagctgct attttggcat
     1261 tatctaactg gtttccagat aaacctctgg ttgatcccac ctgtggatcg ggaacgtttt
     1321 gcatcgaggc agccctgatt gggatgaata ttgctccagg atttaaccgt tcgtttgctt
     1381 ttgaagaatg gagctgggta gataaagaca tggtgcaaca agttcgtgat gatgccgaac
     1441 aagaagctaa ttatgaaatc gaattagata tttctggctt tgatatcgat ggccgcatga
     1501 ttgagattgc taaaagtaat gctgaagaag ctggtttatc tgatgtgatt acctttaagc
     1561 aaatgcgtct gcaagacttt cgaacagaca aggttaatgg tgtagtgata tcaaatcctc
     1621 cttatggaga acggttgctt gatgacaaag cggttgacat cttgtataat gaaatgggac
     1681 aaacctttgc accattgaaa acatggagca aatttatcct tacaagtgat gaactttttg
     1741 aactaaaata cgggcaaaaa gctgataaaa aacgtaaact ttataacggc accctaaaag
     1801 ttgatttgta ccaattttat ggggaaaggg taaagcgaca tctgatatag agaggtagta
     1861 agcgtgtcag aagaaagcaa agaagtcgaa gtaacaaaag aatcgcaaac attggggctt
     1921 aatgaagcca agtcaatgac aattggtgaa gctgtacgca aacagtcaga gataaaagca
     1981 ggtgttacta aggatgatag tattttagat aagtacatta agcaacaccg tgacgaagtt
     2041 tcttctcaaa agtttgatgc taagtataca gagcttgata cagctagttt agataacttt
     2101 atcaaaaaac aaagagaagc attgagtaaa gccggtcttg ttgatgacga acctgtgtct
     2161 gcggagtctg ctgagcaaga tagcactttg gttgaagaag tagctgaaga cttagctcca
     2221 atggagacta ctgcggtggt aactgggatt ccggtggaag cgacagtccc tgtgttagat
     2281 ctagatccat cagagagggt cattccggaa ccacagataa ccaaagaaga gccaaaaaga
     2341 gatcagtttc tttctgaaga tagccaccat ccagccaaac aaaacacgaa aaaaggttgg
     2401 ttaatagctt tatttctact attattggcc attcttgcag ttgtatttgg ttggaaccat
     2461 ttcttgagac aagacagtgg taaaacaact caaacagcaa gtaaacagac aaagacctct
     2521 ttgcagaccg acagtgctaa aaaagcaacg cgcttgaagg ctgctgctaa agctttcgaa
     2581 aaacaatatg gtacttttta tacagatgcc acaaagagca aattaaaaaa tagtgccttt
     2641 gctactttgc cagatttaga agctgccttg aaagctttgg aaggctctgc ttactatgat
     2701 aaggcgaaag caaaagtcga ttcccttaag aaagctattg ctgctattac agcggttaac
     2761 ggtaaatttg tttccgatgt tgttgtagat ggtgaaaagg tttcggctga agtcaaggct
     2821 gatgctaatt ttgatgattt atcaagtgca accttgacaa caggaaatgc aaatcttgat
     2881 gctgtcttgc aggcaagcat cacagaaggt cgtcagcaac tagccagcaa ggctgaggcc
     2941 gctaaagcag ctaatgagca agaagcccag gatcaagcag ctcaaggaca aagtacatca
     3001 gtagcaccat ctggctatgg cttgacaagt tatgatcctg caagcttaca acggcattta
     3061 tcacgtgtac cttacaatca agatgttatt gctgacagag ctaacccatc ttgggcattt
     3121 aatccaggag ttcttgaaaa aattgtagca acctcacaag ctagaggtta tatttcaggc
     3181 aaccaatata ttttagagcc ggttaatatt attaatggca atggttacta taatatgttt
     3241 aagcctgatg gcacttatct gttctctatt aattgtaaga cgggttattt tgtaggaaat
     3301 ggcaaaggtt atgctgatgc tttagattac tgagaaaatc aaaaaaactg agataaggca
     3361 acttgtctca gttttttgat taataaagta tgttgtctta aaatctgcag atagacttgt
     3421 tttttgtaac aagtcaaata ggtcagtagg attgttatgt ttgacaacac actagtatca
     3481 gatgacatgg cgactaaaag gatcgtctga aatcccttga tcaataatca attgagccca
     3541 ttctttggcg gcaaaaaggt tatgatcctt atagtttcca caggattcaa gagttgttcc
     3601 aggaacatct tcccaagtaa tcccagttgc aatttcttct aggctagatt taatcacctt
     3661 ggcaatatca gtagaactgt gttttcccca catgataagg tgaaaacctg ttcgacagcc
     3721 aaaaggagag caatcaatca tcccatcaat gcgttggcgg atgagcttgg caagtaagtg
     3781 ttcaatggta tgcaaaccgg ctgtttcaat agaattttgg ttaggctgta ctaggcgaac
     3841 atcaaaattt gtaatacgat cgcccttggg tccaaattct tcagaaataa gacgaacata
     3901 aggggccttt acaatagtat gatctagctc aaaactttcg acgataactt cttttgtcat
     3961 ttcaatctcc tttgttttac actttatcat aacacaaata gtgagaaagt ggctacttgt
     4021 ggaggagaaa aggctaagaa aacagatcaa aggctatcac aatgacctgt gcgataaaag
     4081 tacttacaga aacagtaata aaggtttctt taaaagcatt tttgtggtta agtcctgtca
     4141 atgctaagaa ggtgagaaag acaccacttt ggggcacaat ggtaaagata ttcgaagcaa
     4201 tagtggcaac acggtgaatc atttcgggat taaggccttg atctaaataa tactgggcaa
     4261 agttaggcat gacaattcct agagcacctg aagaagaacc ggtaatagct gacatagagg
     4321 aagtcaacac ggctaaacta ataagaggat taccgggaat attcaaaatg agatctgaga
     4381 agaatgtgaa acctggggca atcatcacaa cggctccgaa ggcaactgca gatgctgtcg
     4441 caaaaatagg tgcaatagag ctgctagctc ctaaattaaa gacagcaatc ttattaggaa
     4501 taaactgtct aaagagccag cttgcggtga gaatggcagc taaaagtgct atgaaaataa
     4561 tgttcttctt aaaaaaatcg tttccgaaaa ggctaccggt tagggcgata ataatgagca
     4621 acaataaagg taagatactg gcaaggaaat gtgggagatt ttttgttttc acttgtatct
     4681 cattgtcaaa agcataagtg gcataagttt caccccttgc catacttttt gctagacaat
     4741 atttcatata aaagagccca aaagtcacgc agccaattga gccaacgata ctcggaatgg
     4801 cggcagcagt tagtgaggtg tccaagtatt gaataggaat aacattttga atggcaggtg
     4861 ttccgggtaa aatcgtcatg gtaaaagtcg caataccgag ccaaagagga acttgaatta
     4921 agttccaagc caagtccatt tttttgaata ggcttcgtgc aagcggcaag acagcaaaca
     4981 tgacaacaaa gaggctgatt cccccgtaag ttaatatagc actgatcaga aaaatagcaa
     5041 cgagtacttt ataaggacta tcatgaccaa ctttttttaa aatataatct gcaatagaag
     5101 tggttgcacc gcttgtttcc attaatttgg ctaagataga acctaataaa aagatagcaa
     5161 aataattgag aatataagtg gagagtgccc ccataaattg gttgggttct tttccaagta
     5221 aggttgtggt tggatccatt tgattaaaca aaatgaccaa gctggtagca agaggcgcag
     5281 cgatgataat gttaacttct ttcacagaca ggtaaataat aacaataaca cctaccaaga
     5341 ccccgagaga accaataatt tccatagttt tatgacctcc ttattagaaa ataaagagac
     5401 agatcacaag atacagcctc tttatctctc caaatttatt gagcagtgta gccaccgtct
     5461 aagatacagg cttgacctgt aacacctttt gccttatcac tggcaaggaa agacacatag
     5521 tctgcaattt cttgaacgtc aatgaggcgt ttttgaggaa caagtgggta tagaacttct
     5581 tcaagaacat tttcaagggg aatacctctt gttttggaaa ggtcttcaaa ctgtccacga
     5641 accagtggtg tgtcaacata tccaggacaa atggcattga ccgtaatgcc tgaatcagca
     5701 gcttctaagg cagttacttt ggtcagaccg atcaagccgt gcttagctga attgtaggcg
     5761 gatttgccag caaaaccaat gacaccattg atagaagcca tattaataat acgaccgtgt
     5821 ttttgagctt tcattgtagg aaaagcacgt ttaatggcaa taaatggtgc tgtcaacatg
     5881 attttaatca tgaattcaaa tttttcagtt ggaaaatctt caatcatggc aacatgttga
     5941 aggcctgcgt tgttaaccaa aatatcaata cgaccatatt tttcaacaac cgtatcaata
     6001 gcagcattga tggcttcttc tttggtgaca tcgcacacaa cactaaaagc gtcatagcct
     6061 tctttagtat aatcagcaac aacctgatcc agtttttctt gattaatgtc agaaaaaaca
     6121 accgttttac cttcttttaa taaggtttcc ccgatttgtt ttccaattcc gcttgcagca
     6181 ccagtgataa atgcaacttc tttagtatgt gtcatgatac tatcctcgtc tttcttttgg
     6241 ttagtcttta gtttgtttta tcaattaggg taacttcggt aacgttttgc acgtcttcaa
     6301 aggtgtaatc ggggttaatt tcaagggcgc atagaccttc atcttgatat tcaaagactc
     6361 ccatttcagt aatgattagg ttaacaacat tttgagcagt cagaggaagg gtgcatttgt
     6421 ctaaaatttt tgctttgcct ttattggtat gctccattgc aacaatcact tttttagcac
     6481 ctaccaataa gtccatggcc cctcccatcc caggaaccat ttttccagga atcaggtaat
     6541 tagcaatgct agcttcttta tcaacttgta aggcacctaa gacggttgcg gccacatggc
     6601 cgcctcgaat aataccaaag gaagttgagc tatcgaagaa agcccctcca ggagcaatac
     6661 taacaggttg cccaccagca ttaacaatag taggatcgta gtgatcatct gttaatccag
     6721 tgagaccgac aaaaccattt tcggactgca gggtaatggt tacgccttta ggaatatagt
     6781 tggctacttt tgttggcaaa cctatgccta agttgaccag tgtattgtgt tctaattctt
     6841 gtgcaacgcg cttagcaata cgtgtttgaa tctcttcttt tgaaagaaca gtagctgatg
     6901 ttcccatgtt aggctccttt cactaagtag ttaacaaaaa tgccaggagt atggacaaaa
     6961 ttaggatcca tttgccccac gtcaacaatt tctcttgctt caacaatggt ggttttcgca
     7021 ttagcagcca tcacatgatt aaagttattt tcagaacctg cgtattgcaa attgccattt
     7081 ttatctgcct tgttagcaaa aataagtgcg acatcagctt ttaaaggcgt ttcaagaagg
     7141 taatctttgc cgtcaatagt aatgacttct tttcctttgg caacttcggt tcctattcca
     7201 gtaggtgtta agaaaccgcc tagcccaaaa ccgccactgc ggatgcgctc agctaatgtt
     7261 ccctgaggta caagatcaat tacagtctcg ccctcagtca tttgtcggcc cgcttcacgg
     7321 ttgagaccga tatgtgatgc aataatagtt gaaaactgtt tattggcaac catcttacca
     7381 acgcctttgt caggaaaacc ggcatcgtta cagattaaag tgaggtcttt gacacctttt
     7441 tccacaagtg catcaattaa tttttctggt gtgccatttg tcatgaagcc accaaccata
     7501 atggtatcac catctttaac gtgagctacc gcttctttta ttgtgatttc tttacacatt
     7561 ttttctacca actttcacga gactaagtat tgtttttcac gataacagca gttccttgtc
     7621 ctccaccaat gcaaagtgtt gctagtcctc tggttacttg gcgttttttc atttcgtgga
     7681 ttaatgtgac tagaatgcga gcgccactgg caccaatagg atgacctaaa gcgatggctc
     7741 ctccattaac attcacgatt tctgggttta actttaaatc ttttaaaacc gccaaggact
     7801 gtgctgcaaa tgcttcattg gactccacta agtccaaatc agaaattgtt aatccagctt
     7861 tcttaaggac tttttgagtg gcaggaattg gtccagctcc catcagttca ggtgcaacac
     7921 ctgcactagc atagctttca atagtgacta aaggtgttaa ccctaattca agagcttttt
     7981 cttcagtcat taacatcaat aaggctgcac catcgtttat tcctgaggca ttcccagcag
     8041 taacggttcc ttctttaggg aggaaggcag gacgtaattg ctgtaatttt tcaaggcttg
     8101 tgtcaacttt tgggtattca tctgttgtaa caattaaagg gtctttgcgt cgttgaggca
     8161 cagagacagg gacaatttcg tcagcaaaac gatgattttc taatgcctta gcagcttttt
     8221 cttggctttc taaagcaaat tgatcttgct cttctcttga gatgccatat ttctgagcaa
     8281 cgttttcagc agtgataccc atatgataat tgttgaaagc atctgtcaat ccatcatgaa
     8341 caagagtatc aatcaattga ctattaccaa gtcgctgtcc aaaacggtgc tgagttgaca
     8401 cataggctgc ctgtgacatg ttttcgacac caccagcaat aacgatatca gcgtcaccaa
     8461 gcatgatgct ttgcgtagct aattggattg ctttaagtcc tgaaccacag accatatcaa
     8521 gcgtgaaggc tgttttatca ctagggatgc cagagtgaat agcaatctgt ctggcaacgt
     8581 tttgccctaa tcctgcatga aggacgtttc ctaggatgac ttcatcaacc atttcaggct
     8641 ttacttgcgt tttatcaagg atttttttca gtacagttgt tccaagttca acagcagaga
     8701 cacttttaaa ggagcctcca aaagctccga taggagttcg taaagcttca acgataacta
     8761 tgtttttcat aaaataaaca tcctttcatt tttagaaagc gttttctgta tctctaagat
     8821 aacaagaaac cgctcaaaat aaaaatatag aatttttata agcatataaa tttttttgat
     8881 agatttggca atagcaatta aaaatcactt atgtcataat ggaattaata ctatcggaaa
     8941 aaagactaat agacaagggg ataaccattt ggatattaaa caaattcgtt attttatcgc
     9001 aatcgttgaa aatcatttta acttgagcca ggcggcggaa ctgttgtatg tgtcgcagcc
     9061 gacacttagc atgatgatca atgattttga aaaaagggaa aatgttaagc tcttcaaacg
     9121 aaaaagaggt cgtattattg ggttaaccta tcttggtgat aattattata aggacgctca
     9181 aaaggttctc agtctctatg atgacatgtt tttgaaattg catgatcaca gtaaagggtt
     9241 aaagggaagt atcaatattg gtattcctcc tcttatttta tcagttgttt tctcagaagt
     9301 aatgccaaaa ctgattttag aaaatccggg aattcaattc aatgttaagg agattggtgc
     9361 ttaccaacta aaaaatgaac tactcgtggg aaatgttgac gtcgcagtct tgttatcccc
     9421 aacaggcatc gctgataatt tggttgagac ttatgaaacc cagagatcag aattatctgt
     9481 atgcctgtct ccacgtcatc gtttagcttc caaaaaagtt attcagtggg aagatttaac
     9541 agatgaacaa cttgccctat ttgatcctag tttcatggtt caccatttag tgcttgaagc
     9601 ttgcgagcgt caccaagtca gacctaatat tattttgacg tcctcttcct gggattttat
     9661 gcttaattct accaagatta atcacaacgt cttaactatt tgtcccaaac ctattacaga
     9721 attataccaa ttaaaggaca ttaagtgtat tccaatggaa cgtcccattt cttggcgtgt
     9781 tgtcttaaca cgcctccgga aaaaaagtaa ttctgaaata gaagcctaca tcatggacga
     9841 cttgctacag tcttttttta agtagggttc tcaaccactt ggcccagatg ttaatttttt
     9901 taaaaatgaa ttggaattgt gtaaacgctc aatttatgat aaactaagag gtagctttct
     9961 attaatgaaa tctagaatat attgaggtac aaacatggtt aatattattt tattaattgt
    10021 ttctgccctc attggtttaa tattaggtta tgcacttatt tcgattagac tcaagtctgc
    10081 gaaggaagct gcagagttga ctcttttaaa cgctgaacaa gaagctgttg atattcgtgg
    10141 caaagcagaa gtagatgctg aacacatcaa aaaaacagct aaacgtgaaa gtaaagcaaa
    10201 tcgtaaagaa ttacttttag aagcaaaaga agaggcaaga aaatatcgtg aagagattga
    10261 acaagaattt aagtctgaaa gacaagagct taaacaactc gagacacgct tagcggagcg
    10321 ctccttaact cttgaccgta aagatgaaaa cctatcaagt aaagaaaagg tactagatag
    10381 taaagaacaa agtctgaccg ataaatctaa acacattgat gagcggcaac ttcaagtaga
    10441 aaaacttgaa gaggagaaaa aagcagaact ggaaaaagtt gctgcgatga cgattgcaga
    10501 agcgcgtgaa gtgattttaa tggagacgga aaacaaactg acccatgaaa ttgcgacgcg
    10561 cattcgagat gccgaacgtg acatcaagga ccgaacagtt aaaacagcca aggacttgtt
    10621 agcgcaagcc atgcaacgcc ttgctggtga gtatgtgact gaacaaacta ttaccagtgt
    10681 ccatctccca gacgacaaca tgaagggccg aattattgga cgtgaaggcc gtaatattcg
    10741 tactttagag agcttgactg gcattgacgt tattattgac gatactcctg aagttgttat
    10801 cttatcagga tttgatccta ttcgacgtga aattgctcgt atgaccttgg aatctctgat
    10861 tgctgatggt cgcatccatc cagctcgtat cgaggaattg gttgagaaaa atcgtcttga
    10921 aatggataat cgtattcgtg agtacggtga agctgcagcc tatgagattg gtgcaccaaa
    10981 ccttcatcct gatttgatta aaatcatggg acgcctgcaa ttccgtacct cgtttggtca
    11041 aaatgtccta cgtcactctg ttgaggttgg taagttagct ggtattttag ctggtgagtt
    11101 aggtgaaaat gttgctcttg cccgccgtgc tggtttcttg catgatatgg gtaaagctat
    11161 tgaccgtgag gttgaaggta gtcacgttga gattggaatg gaatttgcac gtaaatacaa
    11221 agaacatcca gttgttgtca acactattgc tagccaccac ggagatgtgg agccagattc
    11281 tgtgattgct gtgctagtag ctgcagcaga cgctctcagt tcggctcgtc caggcgctcg
    11341 taatgagtca atggagaatt acatcaagcg tcttcgtgat ttagaagaaa tcgcgacaag
    11401 ttttgatggg gtacaaaata gttttgctct acaagctgga cgtgagattc gtattatggt
    11461 tcaacctgaa aaaatttcag atgatcaggt tgtcattttg tcgcataagg taagagaaaa
    11521 aattgaaaac aatctagatt acccaggaaa tattaaagta actgttattc gtgagatgag
    11581 agcggttgat tatgccaagt agaaaaagag gaattatcct ctttttcttt atgacttgcc
    11641 tttgaaaatg ttcttgaaaa agactagcct ttttgataaa ctattaaaaa gcaactaata
    11701 aggagaatac aatgtctgaa cgcggtttgt taatcgtctt ttctggccct tcaggtgtcg
    11761 gaaaaggaac ggtaagacaa gaaatttttt caacgccaga tcataaattt gaatattcgg
    11821 tttcaatgac gactcgtccg caaagaccgg gcgaggttga tggggttgat tatttcttcc
    11881 gcacacgtga agaatttgaa gaactcatta aaactggtca aatgctagag tatgctgaat
    11941 atgttggtaa ttactatggc acccctttaa cttatgttaa tgagactctt gataagggaa
    12001 ttgatgtctt tttagaaatt gaagttcaag gagctcttca ggttaagtca aaagtaccag
    12061 atggtgtttt tgttttttta acaccaccag atttagatga attagaagac cgcctggttg
    12121 gtcgtggtac agatagtcaa gaagtcatag ctcaacgtat tgaaagagca aaagaagaaa
    12181 ttgcccttat gcgcgaatac gattatgcag ttgttaatga tgaagttgcc ttggcagctg
    12241 agcgggttaa gcgtatcatt gagacagaac actttcgtgt ggagcgcgtg attggtcgtt
    12301 atgataaaat gattaaaata acaaaaaatc cctttaaagc taaatagaaa agagtaagca
    12361 ttatgatgtt aaaaccttcc attgatacct tattagataa agtaccatcc aagtattcac
    12421 ttgtgatttt acaagccaaa cgtgcccatg aactagaagc tggtgccact ccaactcaag
    12481 aatttaaatc agtgaagtca acacttcagg cacttgaaga aattgaatca ggaaacgttg
    12541 tgattcaccc agatccttca gctaaacgtg aagctgtccg tgctaagatt gaggcagaac
    12601 gcttggccaa agaggaagag gaacgtaaaa tcaaagaaca aattgccaaa gaaaaagaag
    12661 aagaaggaga aaaaatctaa ggtttcagaa atcttagatt ttttcatatt tagtacgatg
    12721 tccatgacag aaaaggagaa gcaaatggaa aaagtagctc atgtgattgt agatattcca
    12781 ttgatgcaaa cggacaagcc attttcttat ggtattccaa aagagttagt ttctctcgtg
    12841 cagcttggtt ctcgtgtgca tgttcctttt ggaaaaggga atcgtttgtt gcaaggcttt
    12901 attattggtt ttggccaaga agattcaagc tctcttaaac tgattcagac cgttttagat
    12961 cctgagccag tcctaaacca agaacaattg acccttgctg atcagcttcg aaaaacagtt
    13021 ttttcttata aaatcacgct cttaaaagca atgattccta atcttcttaa ctctaactat
    13081 gataaagtgc taaggccaga gtcaggtcta aaaaagtctg atagggactt tctttttgaa
    13141 ggaaaaccat ctgtcttata ttctacctta gaccgcgaga agggaaaaat agctcttaag
    13201 ggcatacaag ctgggcatat tactgttagt tatttagcca aagacaagaa aaacctaaag
    13261 acagaaaaat attatcacgt cgacttagac gccttagcgg tacatcccat ctctagtcgt
    13321 gccaaaaagc gtcaattatt aaaagactat ctgctaaccc atacgaagga agctaaactt
    13381 gcaacgcttt atcaagcctt ttctagagat gtggtgactt actttgtgac aaatcactta
    13441 attcgtattg acgaacgtcc aattgatcgc agtgagtcct attttgacca gatcaaacca
    13501 tcttcttttt taaccttaaa tgaacagcag gcgtcagctg ttactgaaat tgttgaacaa
    13561 attgggaaac cgagtaaacc ttttttgatt gaagggatta caggttcggg taagacagaa
    13621 gtttatcttc atatcatcga agcggtttta aaacaggata agacagccat tgttttagtg
    13681 ccagaaattt cgttgactcc tcagatgacc tctcgtttta tttcccgttt cggaaagcaa
    13741 gtggctatta tgcattcagg cttatcagat ggtgaaaagt ttgatgagtg gcgcaagata
    13801 aaaacaggac aggctaaggt tgtggttggt gcccgctctg ctattttctc cccactagag
    13861 cggattggtg caattattat cgatgaggag catgaatcaa cctataaaca agaaagtaat
    13921 ccacgatacc atgctagaga ggttgccctg ctcagagcta aacaccatca agcagttgtg
    13981 gttatgggct ctgctacgcc aagtattgaa agccgagcaa gggctagtaa gggtgtctat
    14041 cattttattc aattaacgca aagagcaaac cctctcgcta agattccaga agtaacgatt
    14101 gttgattttc gtgattatat tggacagcaa gcagttagta attttacccc atatttgatt
    14161 gataaggtta aagaacgttt ggttaaaaaa gaacaagttg tcttaatgtt aaatagacgt
    14221 ggttattcta gttttgtgat gtgtcgggac tgtggttatg tggacaaatg tcccaactgt
    14281 gatatctctt taacccttca catggatact aaaactatga attgccatta ctgtgggttt
    14341 caaaaaccta tcccaattac ttgtcctgag tgtcatagca atagtatccg ctattatgga
    14401 acagggacgc aaaaagcttt tgatgaattg caaggggtta ttccagaagc taagatcttg
    14461 agaatggatg ttgacaccac acgaaaaaaa agaagtcaca aaaccatact agatagtttt
    14521 ggtcgccagg aagcagatat tttgttaggg acgcaaatga ttgccaaagg actggatttt
    14581 ccaaatgtga ctttagttgg ggtactaaat gcagatacct ccttaaattt gccagatttt
    14641 cgagcgtcag aaaaaacgtt tcaattgtta acacaggttg ctggtcgggc aggccgtgcc
    14701 cataagccag gggaagtctt gattcaaact tataatcccg atcactatgc aattcagttg
    14761 gctaaacaac aagattttga agccttttac cgttatgaaa tggcgattcg ccgtcagatg
    14821 gcttatcccc cttactactt tacagtgggt attacgcttt cgcatcgttt agaagcatcg
    14881 gttgtaaaaa aagcttatca agttacagaa ctgctaaaga gtcacttatc gaataatatt
    14941 aaaatattag gaccgactcc taagccaatt gctagaacac acaatttata ccactatcaa
    15001 attttgttaa aataccgttt tgaagataac ctagaagaga cgctcaatcg tattcttgat
    15061 tggagtcaag aagcagataa tcgtcattta aaactaatta ttgattgtga accgcaacaa
    15121 tttttataag gtaaatcact aaatctagga tgatcagtta tgatataata aaaatacaaa
    15181 gaggaaagtc atgataaaat tattatttat gggaactccc caattttcag caactgtatt
    15241 gaagggcttg ctagataatc ctgcctatga gattttagga gtggttactc agcctgatcg
    15301 agctgttgga cgcaaaaaag atattaaggt aacacctgta aagcaattag ctcttgagca
    15361 tggaatatca atctatcagc cggaaaaatt atcaggctct caagaactca ttgagattat
    15421 gggacttggt gcagatggta ttattacagc agcatttggc caatttttac caacactatt
    15481 acttgattca gtttcatttg ctattaatgt acatgcatca ttgttaccta aatatcgagg
    15541 tggagcacct atccattatg ctattatgaa tggagataaa gaggctggcg ttactattat
    15601 ggaaatgatt aaagaaatgg atgcaggtga catggtcgct aaagcaagca ctccaattct
    15661 tgaaaccgat aatgtaggta ctctttttga gaaattagcc atcattggtc gagacttact
    15721 tttggatagt ttgccagctt atctttctgg agaattaaaa cctattcctc aagatcacag
    15781 ccaagctact ttttcaccta atatctctcc agaacaagaa aaattagatt ggacaatgtc
    15841 taatcaagaa gtgttcagtc atattcgagg tatgaaccct tggccggtag cgcatacgtt
    15901 tttagagggg caacgtttaa aaatttacga agctcaacta gctgaaggag aaggattacc
    15961 tggacaagtt attgtcaaaa ccaaaaaatc tttggtaata gctactggac aaggagcttt
    16021 gtccttgatc gttgtgcaac cggctggtaa acctaaaatg tcaataatag actttttaaa
    16081 cggcattggt agaaaacttg aagtagggga catcattggc agataattgg aaaaaaagta
    16141 cgcgaggcaa agccctcctt gtcattgaag ctatttttga tcagggggct tacacaaaca
    16201 tagcgcttaa tcagcagcta agcagtaaag ctttatctgc taaggacaga gctctattaa
    16261 ctgagattgt atatggtacc gtttcacgta aaattagctt ggaatggtat ttagcccatt
    16321 atgtcaaaga ccgagataaa ttggacaaat gggtttatta tctccttatg ctcagtcttt
    16381 atcaactgat ttatttagat aagttaccag cgcatgccat tgttaacgat gcggttggta
    16441 ttgccaaaaa tcgtggcaat aaaaaaggtg cagagaagtt tgtcaatgct attttacgtc
    16501 aattcaccag ccatcctctc ccagatatgg aaacaattaa gcgtcgaaat aagtattatt
    16561 cggtcaagta ttcactgcct gtttggttgg ttaaaaagtt agaagaccaa tttggctccg
    16621 acagagcagt ggctattatg gagagtttat ttatgcgtag taaggctagt actcgcgtga
    16681 cggacccttt gaagttagaa gaagttgctg aggctttaga tgcagaacgt tcccttttat
    16741 cagcaactgg tttaaccaaa gcgtcaggtc attttgcagc cagtgattat ttcacaaatg
    16801 gggacatcac tattcaagat gaaagcagtc agcttgtagc tcctacttta aacattgacg
    16861 gtgacgacat aattcttgat gcgtgcagcg ctccaggggg taagacctct catatagcat
    16921 cttacttaaa aacaggtaaa gttattgcct tagatctcta tgatcataaa ctagagttgg
    16981 tgaaggaaaa tgctaaccgc ttaggagttg ctgataacat tgagacaaga aaactagatg
    17041 ccagagaggt tcatcgtcat tttgaaaagg atagctttga taagattttg gtggatgctc
    17101 cttgttcagg aattggtttg attcgtcgaa aacccgatat caaatataat aaagaaagtc
    17161 aagattttaa tgctctacag gctatccagc tagagatatt gtctagtgtt tgtcaaacct
    17221 tgcgtaaagg tggtataata acatacagta cttgcactat ttttgatgag gagaatcgtc
    17281 aagtcatcga agcattttta cagagtcatc ctaattttga acaagtaaaa ctgaaccata
    17341 cacaagcaga tattgtcaag gatggttatc ttattatcac accagaacaa taccagactg
    17401 atggcttctt tattggacaa gtcagacgag tcttgtagta ttttaggagg aaactgactg
    17461 agtcagaaaa aacgtatgaa gatttcatta aaaacagaca ttgggcaaaa acgctcaaac
    17521 aatcaagatt ttatcaataa gtttgataat aaaaaaggca tcactttagt gattttagca
    17581 gacggtatgg gtggccacag agctggaaat attgccagtg agatgacagt cacagacctg
    17641 ggaagagaat gggtaaaaac agacttcaca gagttaagtc agattagaga ttggttattt
    17701 gaaactatcc agtcagaaaa tcaacggatt tatgatctag gccaatcaga agactttaaa
    17761 ggaatgggaa cgactgttga agcagtagct ttagttgaat ctagcgccat ttatgctcat
    17821 attggtgatt cacggattgg tcttgtccat gatgggcatt ataccttatt gaccagtgat
    17881 cattctttag tgaatgagtt ggttaaggca ggacagatca cagaagaaga agctgctagc
    17941 catccacaaa gaaatattat cactcaatct attggtcaag cttccccagt agagcctgat
    18001 ttaggagtga gggttttgga gccaggggat tatcttgtca ttaacagtga tggtctaacc
    18061 aatatgattt caaatgatga aattgtgact atcttgggca gtaaggttag tcttgatgaa
    18121 aaaaaccaag aaatgattga cttagctaac ctaagaggag gcctagataa tattacaatc
    18181 gctttggttc acaacgaaag tgaggacgtc gaatgattca gattggcaaa ttatttgctg
    18241 gtcgttatcg cattctgaaa tctattggcc gcggtggtat ggcggatgtt tatttagcaa
    18301 atgatttgat cttggataat gaagacgttg caatcaaggt cttgcgtacc aattatcaaa
    18361 cagatcaggt agcagttgcg cgtttccaac gggaagcgcg ggccatggct gaattgaacc
    18421 atcccaatat tgttgccatc cgggatatag gcgaagaaga cggacagcaa tttttagtaa
    18481 tggaatatgt ggatggtgct gacctaaaga gatacattca aaatcatgct ccattatcta
    18541 ataatgaagt ggttagaatt atggaagaag tcctttctgc tatgacttta gcccaccaaa
    18601 aaggaattgt acacagagat ttaaaacctc aaaatatcct actaactaag gagggggttg
    18661 tcaaagtaac tgatttcggc atcgcagtag cctttgcaga aacaagcttg acacaaacta
    18721 attcgatgtt aggcagtgtt cattacttgt ctccagaaca ggctcgcggc tccaaagcga
    18781 cgattcaaag tgatatttat gcgatgggga ttatgctctt tgagatgttg acaggccata
    18841 tcccttatga tggcgatagt gctgttacga ttgccttgca acattttcaa aagcctcttc
    18901 catctattat cgaggagaac cacaatgtgc cacaagcttt ggagaatgtt gttattcgag
    18961 caacagccaa gaaattaagt gatcgttacg ggtcaacctt tgaaatgagt cgtgacttaa
    19021 tgacggcgct tagttataat cgtagtcggg agcgtaagat tatctttgag gatgttgaaa
    19081 gtaccaaacc cctccccaaa gtggcctcag gtcccaccgc ttctgtaaaa ttgtctcccc
    19141 ctaccccaac agtgttaaca caggaaagtc gattagatca aactaatcaa acagatgctt
    19201 tacagccccc caccaaaaag aaaaaaagtg gtcgtttttt aggtacttta ttcaaaattc
    19261 ttttttcttt ctttattgta ggtgtagcac tctttactta tcttatacta actaaaccaa
    19321 cttctgtgaa agttcctaat gtagcaggca ctagtcttaa agttgccaaa caagaactgt
    19381 atgatgttgg gctaaaagtg ggtaaaatca ggcaaattga gagtgatacg gttgctgagg
    19441 gaaatgtagt tagaacagat cctaaagcag gaacagctaa gaggcaaggc tcaagcatta
    19501 cgctttatgt gtcaattgga aacaaaggtt ttgacatgga aaactacaaa ggactagatt
    19561 atcaagaagc tatgaatagt ttgatagaaa cttatggtgt tccaaaatca aaaatcaaaa
    19621 ttgagcgcat tgtaactaat gaatatcctg aaaatacagt catcagtcaa tcgccaagtg
    19681 cgggtgataa atttaatcca aacggaaagt ctaaaattac gctcagtgtt gctgttagtg
    19741 atacgatcac tatgcctatg gtaacagaat atagttatgc agatgcagtc aataccttaa
    19801 cagctttagg tatagatgca tctagaataa aagcttatgt gccaagctct agctcagcaa
    19861 cgggatttgt gccaattcat tctcctagtt ctaaagctat tgtcagtggt caatcccctt
    19921 actatggaac gtctttgagt ctgtctgata aaggagagat tagtctttac ctttatccag
    19981 aagaaacaca ctcttctagt agctcatcga gttcaacgtc aagttcaaac agttcttcaa
    20041 caaatgatag tactgcacca ggtagcaaca ctgaattaag cccatcagaa actacttctc
    20101 aaacacctta acaaagaata gttagtctca tgttttattg gcatgccact aactgttctt
    20161 tttaactact cgtcatataa agatgcaatt ttccaggaat tctgataaaa tacaaagaaa
    20221 taaggggaaa ctaatgaaaa aatttcaatt ctttttactt atcgagtgta tactgctagc
    20281 tatgggtatc atgacaatct tggataatga tttatcaagt tttatcctaa ttctagttct
    20341 tatcttatta gcattacgct tttataacca agatagccgc aataattttt tgctaactgt
    20401 cagtttattg tttctttttt taattttcat gcttaatccc tacattatca tggctgttct
    20461 tttggggata gtttacattt tcattaacca tttttcacaa gtcaaaaaga agaatcgctt
    20521 tgccttgatt cgtttcaagg aagaaaagat tgaagtgaat aataccaagc atcaatggat
    20581 tggtactgct aattatgaaa gtgattatta ttgttttgat gacattaaca tcattcgaat
    20641 ctcaggaaat gacacggttg acttaaccaa tgttattgtg acaggaatgg ataatatcat
    20701 tgtgatacgt aaaatttttg gaaatacgac tatattggta cctattgatg tcactgttac
    20761 tttagatgtt agttccatat acggaagcgt tgattttttt agatgtcagc aatatgattt
    20821 gcgcaacgaa tctattaagt taaaagaaac ggataaccaa tcccttaaaa aagtcaaaat
    20881 tatcgttaca actattgccg gaaatgttga ggtggatagg cgatgaaaaa acgttactat
    20941 gctcttgttt ggctctactc aaccattacc attttatcta ttgtttttgt ggtcatggat
    21001 aatttaggaa tcactattaa ctaccttcgc aatcatttat ggcaggtcga acgtctaggg
    21061 ttttcgattt tattgttgat tgtttctgtg actttattat tgttgttatt gtggattatt
    21121 atggatgata acagtaagcg taacatcaac caaaatctaa aatatattct caataatcga
    21181 cgactctatc tagatgagac atcagaaatc aatactaatt taagtcgact ttctaaaaaa
    21241 atgtctcacc tgactgctaa catgcaaaag aaagaaagtg cttatattct tgatagccaa
    21301 gaagttgtaa aacaagagcg caaacgaatt gcaagagatt tacatgacac agtcagtcaa
    21361 gagctattcg cttcgtcatt gattttatca ggaatttcga tgagtttgga acaactggac
    21421 aaaacacaat tacaaacaca gttaacaacg gttgaagcaa tgttgcaaaa tgctcaaaat
    21481 gatctacgca ttctcctctt gcatcttaga cctaccgagc tagctaatcg gactttatct
    21541 gagggccttc atatgattct taaggaatta acagataaaa gtgatattga agtcatttat
    21601 aaggaaacca ttgctcagct tcctaaaaca atggaagata atctttttag aattgcccaa
    21661 gaattcatta gcaacacgtt aaaacatgct aaagctagtc gaattgaagt ttatctcaat
    21721 caaacctcaa cagaattaca attgaagatg attgatgatg gtgtaggatt tgatatggat
    21781 caggtaaggg atttgagtta tggtctgaag aatattgaag accgtgtcaa tgatttagca
    21841 ggaaacctac atttaattag tcaaaaaggc aaaggagttt ccatggatat tagactgccg
    21901 atagtgaagg gaaatgacga tgagtaagat aaaagtgata ttggtcgatg atcatgaaat
    21961 ggtccgcatg ggactcaaga gttttttgaa tttacaagct gatattgatg tcgttggtga
    22021 agcctctaat ggacgtgaag gggttgattt ggcattggct ttgaagccag atgttttggt
    22081 tatggatcta gtgatgccag agttaggcgg tgttgaggca actttagaag tcctaaaaaa
    22141 atggaaagag gctaaggtac ttgtgttaac ttcctatcta gataatgaaa agatataccc
    22201 tgtcattgat gctggtgcta aaggttacat gttaaagact tctagtgcag ccgaaattct
    22261 aaatgctatt cggaaagttt caaaaggtga attggccatt gaaacagagg tggacaaaaa
    22321 aattaaagcg catgaccaac accctgattt gcatgaagag ctaaccgcgc gcgagtatga
    22381 tattttacat cttttagcta aagggtatga taatcaaact atagcggatg aacttttcat
    22441 ctccttgaaa accgttaaaa cgcatgtatc taatattctt gctaagctag aagttgatga
    22501 ccgaactcaa gcggttgtct atgcttttcg acaccattta gtcccccaag acgataatta
    22561 atgctataat atagggtata gcaaattgga ggaaagtatg gacgcaaaac taaaatacaa
    22621 agctaaaaaa atcaaaatgg tgttctttga tattgatgat accttgaggg tcaaagatac
    22681 gggttatatg cctgagtcga ttcaaagagt atttaaggct ttgaaggcaa aaggcatctt
    22741 ggttggtatt gcttcaggtc gcgctcgtta tggtgttcca caagaagttc aagatttaca
    22801 tgctgactat tgtgtcaaat taaatggagc ttatgtcaaa gacgatgcta aaactattat
    22861 tttccaagct ccgattccag cagatgttgt tgtggcctat aaaaaatggg cggatgatat
    22921 gggcattttt tatggaatgg cgggtcgtca tgaagcggtg ttgtccgctc gaaatgacat
    22981 gattagcaat gctattgata atgtctacgc tcaactagag gtctgtccgg attataatga
    23041 gtatcatgat gtttaccaaa tgtggacctt cgaggacaag ggagatggct tgcagttgcc
    23101 tgctgaatta gcagagcacc ttcgtttggt cagatggcac gataactcat cagatgttgt
    23161 cttgaaaggt acgtcaaaag cgctaggtgt ttcaaaagta gttgaccatt taggattaaa
    23221 acctgaaaac attttggtgt ttggtgatga actaaacgac ttagagttat ttgactatgc
    23281 tggtatcagt attgcaatgg gagtatcaca cccacgtttg caagaaaaag cagatgttat
    23341 cacaaaaaaa gttgaagaag atggcatttt atatgccttg gaggaattag gattgattga
    23401 caaagaatta cagtttccac aattggattt agaaaatcat actggaccaa aagtaaccat
    23461 taaaacaaat catggggata tgactttggt cttgttccca gagcattccc ctaagacggt
    23521 tgctaacttc ttaggattag ctaaagaggg atactatgat ggcattattt tccaccgcgt
    23581 tattcctgaa tttatgattc aaggtggtga tccaacagga acaggaatgg gtggacaatc
    23641 catctatggt gaaagttttg aagatgaatt ttcggatgaa ctgtataacc ttagaggagc
    23701 cctttcaatg gcaaacgcag gcccaaatac taatggtagc caattcttca ttgttcaaaa
    23761 tagcaaaatt ccttacgcta aaaaagaatt agaacgtggt ggttggccta ctccaattgc
    23821 agctgcttat gctgaaaatg gtggaactcc tcaccttgat cggcgtcata ctgtttttgg
    23881 ccaattagct gatgacgctt ctttcaaagt tttggacctg attgctagcg ttgaaactgg
    23941 cgcacaggat aaacctaaag aagacgttat cattaaaaca attgaggtat ttgactaatg
    24001 aaaattggcg acaaactgca tggcaccatc actgggatta aaccatacgg agcctttgtt
    24061 gcccttgaca atgggacaac aggtctcatt catatttcag aaataaaaac gggatttatt
    24121 gatgatattg atcaattatt ggctataggc aaccaggttt tagtacaagt aattgatatt
    24181 gatgaatata gtaaaaaggc aagtttatct atgcggacct tagcagaaga aaaacagcat
    24241 tttttccatc gtcatcgtta ttccaacagt cggcacaaga ttggctttag gccacttgaa
    24301 gagcagttac ctcagtggat tgaagaaagc ctgcagtttt taaaaaagaa cactaaaaag
    24361 agtctggaac cttagtagtt ccaaaccctt ttttatttcg tcttaattac tggtacatca
    24421 tagagttcag tcgataaata gcgttcgcca ttatctggta aaatagttaa cacatgtttg
    24481 ccttttccta actgtttagc gacttcaata gcggcgtaaa gagcagctcc agaagaaatc
    24541 ccaaccagga agccttcctt agctcctgtt agtcgagcag tttctaaagc atctttcgat
    24601 ttaacacgga taatttggtc ataggctttg gtatctaacg tgtttgggat aaatccagct
    24661 gatatacctt ggattttatg tggtccaggc tcttgaccag ataagacagc agattcttca
    24721 gcttcaacag catagatagc agtttcaggg ctagcttttt tcaagacatg tgaaacacca
    24781 gaaagtgttc ctccagtacc aacaccagat acgaatgcat ctaaagaaat ctccttaaaa
    24841 gcttccaaaa tttcttgagc tgttgttttt tcatggatgc ttggattggc agggttatta
    24901 aattgcatag gcatccaagc acctagttct attgctaaag tttcagcttt tgcaatagcc
    24961 cctttcatac cttctgctcc aggtgttaag acaagctctg caccataagc ctgaatgatt
    25021 tgtcgtcttt ccaagctcat cgtttcgggc ataacaataa tgactcgata ccctttagca
    25081 gcacctaccc atgcaagacc aatacctgta ttaccgctcg ttggttcgat aataacgtca
    25141 ccaggactta tcagaccttc agcttcagca gcttcaatca tcgataaagc aatacgatct
    25201 ttaacagaag atcctgggtt aaaagcttct aattttacat aaacgtcagc agcttcgttt
    25261 ggaattaaac ggttaagttt gataataggt gtctgaccta ctaattctgt tatagttttg
    25321 taaattttag tcatagttta gcctcttttc ttgatacaaa ataaatacgc tgtatattta
    25381 agtataagga gagatattat taaagtaaaa tacagattta ctatcactct gataataaag
    25441 ccatatcact tttgttgatg attaatcaca ggggaattca ataactttgg atccgatttt
    25501 ctcacagctt atctgaccat gataataatt tgttaaagta tcaatgagat tttccacacg
    25561 ttcagtatca caataaagtg ttgtctttat cgtgtctgaa aacttagttt ctgtttcggt
    25621 taaagccaat tgatctagta agttagagta tagttgatat tggggataag aaagtgtgat
    25681 ttctagtcct atctgctgtt taacttcaat gatgccaaat cgtttaatag ctgttgcggt
    25741 catgttagag tatgctctga ttaacccacc tgttcctaat ttaataccac caaagtaacg
    25801 cgttaccaca actactacat ttgttaaaca ctgcttttct aacacagaaa ggataggtat
    25861 tccagcagtt cctgaaggtt caccatcatc acttgagcgc ttgatctgac tgttgttacc
    25921 gataatcatc gcaaagcagg aatgattagc tttgtaatgt tcttttttga tagcattaac
    25981 aaaattctta ccatcttctt cagtgctcac tcgtttgata tgacaaatga aacgagattt
    26041 tttaatactt tcttcgaaaa aaccact
//
LOCUS       DAUELZ010000013        25835 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.013, whole genome shotgun sequence.
ACCESSION   DAUELZ010000013 DAUELZ010000000
VERSION     DAUELZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25835
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.013"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            125..1339
                     /gene="sstT"
                     /locus_tag="VHI09_000430"
     CDS             125..1339
                     /gene="sstT"
                     /locus_tag="VHI09_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEQ6120333.1"
                     /translation="MKKIYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGRQTNMTLIIFLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASQETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLV
                     GTMVFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKRKKA"
     gene            complement(1394..2068)
                     /locus_tag="VHI09_000431"
     CDS             complement(1394..2068)
                     /locus_tag="VHI09_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEQ6120334.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFIIPKSW
                     EGQSLSELDVRRKYELNIIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(2078..3469)
                     /locus_tag="VHI09_000432"
     CDS             complement(2078..3469)
                     /locus_tag="VHI09_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEQ6120335.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSLEAFAALVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(3538..4251)
                     /gene="rsmG"
                     /locus_tag="VHI09_000433"
     CDS             complement(3538..4251)
                     /gene="rsmG"
                     /locus_tag="VHI09_000433"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEQ6120336.1"
                     /translation="MTPQDFYRTLEEDGFSLASKQKEQFDTYFKLLVEWNTKINLTAI
                     TEKNEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            4401..4958
                     /locus_tag="VHI09_000434"
     CDS             4401..4958
                     /locus_tag="VHI09_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEQ6120337.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            5005..5901
                     /gene="htpX"
                     /locus_tag="VHI09_000435"
     CDS             5005..5901
                     /gene="htpX"
                     /locus_tag="VHI09_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEQ6120338.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMIKALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
     gene            6135..6668
                     /locus_tag="VHI09_000436"
     CDS             6135..6668
                     /locus_tag="VHI09_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEQ6120339.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            6935..7621
                     /gene="covR"
                     /locus_tag="VHI09_000437"
                     /gene_synonym="csrR"
     CDS             6935..7621
                     /gene="covR"
                     /locus_tag="VHI09_000437"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEQ6120340.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            7627..9129
                     /gene="covS"
                     /locus_tag="VHI09_000438"
                     /gene_synonym="csrS"
     CDS             7627..9129
                     /gene="covS"
                     /locus_tag="VHI09_000438"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEQ6120341.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHEVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSNES"
     gene            9344..9838
                     /gene="nrdR"
                     /locus_tag="VHI09_000439"
     CDS             9344..9838
                     /gene="nrdR"
                     /locus_tag="VHI09_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEQ6120342.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            9822..10997
                     /locus_tag="VHI09_000440"
     CDS             9822..10997
                     /locus_tag="VHI09_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEQ6120343.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGDLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            10998..11900
                     /gene="dnaI"
                     /locus_tag="VHI09_000441"
     CDS             10998..11900
                     /gene="dnaI"
                     /locus_tag="VHI09_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEQ6120344.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLSDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYRFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            11963..13273
                     /gene="der"
                     /locus_tag="VHI09_000442"
     CDS             11963..13273
                     /gene="der"
                     /locus_tag="VHI09_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEQ6120345.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            13480..16578
                     /locus_tag="VHI09_000443"
     CDS             13480..16578
                     /locus_tag="VHI09_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ6120346.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSHFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASDTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIKRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQTRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMQDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMN
                     YQGKSGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNESRASMSIEEIKEILGLN
                     TH"
     gene            16820..17422
                     /locus_tag="VHI09_000444"
     CDS             16820..17422
                     /locus_tag="VHI09_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120347.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIAGNEELTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            17462..18790
                     /locus_tag="VHI09_000445"
     CDS             17462..18790
                     /locus_tag="VHI09_000445"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEQ6120348.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDMALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            18836..19318
                     /locus_tag="VHI09_000446"
     CDS             18836..19318
                     /locus_tag="VHI09_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ6120349.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            19430..21004
                     /gene="mltG"
                     /locus_tag="VHI09_000447"
     CDS             19430..21004
                     /gene="mltG"
                     /locus_tag="VHI09_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEQ6120350.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAARRTAQLYAEYKRQDTFQKESIAHNNKTAKHFQAIKDAVMTSEAIKPTLLSE
                     KENSSLKTTSKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            21077..21559
                     /gene="greA"
                     /locus_tag="VHI09_000448"
     CDS             21077..21559
                     /gene="greA"
                     /locus_tag="VHI09_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEQ6120351.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVHEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTS
                     "
     gene            complement(22183..23106)
                     /gene="yidC"
                     /locus_tag="VHI09_000449"
     CDS             complement(22183..23106)
                     /gene="yidC"
                     /locus_tag="VHI09_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ6120352.1"
                     /translation="MRLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMTFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKTPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(23188..23466)
                     /locus_tag="VHI09_000450"
     CDS             complement(23188..23466)
                     /locus_tag="VHI09_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEQ6120353.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGN"
     gene            23727..24464
                     /locus_tag="VHI09_000451"
     CDS             23727..24464
                     /locus_tag="VHI09_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEQ6120354.1"
                     /translation="MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            24503..25003
                     /locus_tag="VHI09_000452"
     CDS             24503..25003
                     /locus_tag="VHI09_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ6120355.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            25018..25707
                     /locus_tag="VHI09_000453"
     CDS             25018..25707
                     /locus_tag="VHI09_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEQ6120356.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFIASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
BASE COUNT         8320 a         4431 c         5219 g         7865 t
ORIGIN      
        1 caatagaaat tgactgaaaa ttcttagcac aaaaaatggt taagatatgt agctcattcg
       61 atttgacgaa tagatagaat tatattatta taaaaaacat gtaactttag taaggatgta
      121 acgtatgaaa aaaatatatg atttatgggt tagggttagt ctaatcaaaa aaataggtat
      181 cggtgttgta ataggagtca tgttgggcat tttggctcct gatcttacag gttttagtat
      241 tttaggaaag ttatttgttg gtggtttaaa ggctatcgca cctttgcttg tatttgcttt
      301 ggtttcccaa gccatctccc atcagaaaaa agggagacag actaatatga cattaatcat
      361 ttttctgtat ttatttggta cctttgcgtc agctttagta gcagtactga cagcttatct
      421 attccctttg acgttagtgt taaatacacc tgtaaataca gaattatcac cacctcaagg
      481 tgttgctgaa gtttttcagt cacttctatt aaaattagtg gataatccga tcaatgcttt
      541 agctactgcc aattatattg gtgtgctatc atgggcaatt atttttggac tagcattaaa
      601 ggctgcaagt caagaaacaa agcatcttat aaaaacagcg gcagaagtta cttcacaaat
      661 agttgtttgg atcatcaatc tggcacctat tgggattatg agtctggtgt tcactacgat
      721 ttctgaaaac ggtgttggaa tcttatctga ttatgctttt ttgatactag ttttagtagg
      781 aacaatggtt tttgtcgcct tagtggttaa tccacttatt gcggttttga ttacacgaca
      841 aaatccttat ccccttgtct taagatgttt acgtgagtca ggtcttacag catttttcac
      901 acgaagttct gcagccaata ttccagtcaa tatgcagtta tgccaaaaaa ttggattgag
      961 taaagatact tattcagtgt caattccact tggagcaact attaatatgg gtggggcagc
     1021 aattaccatc aatgtcctaa cgcttgctgc tgtacacact tttggtattc ctattgattt
     1081 tctaacagcc ctcttactta gcgttgttgc tgctgtctct gcttgtggtg cttctggtgt
     1141 tgctggggga tcgttgttac ttattcctgt agcatgtagt ttgtttggca tttcaaatga
     1201 tcttgcgatg caggtggttg gtgttggttt tattgttggt gttatccagg attcttgtga
     1261 gacagcctta aattcgtcaa ccgatgttct ttttacagct attgctgaaa atgctttttg
     1321 gaaacgtaaa aaagcctagc taggtttaac ataataaaaa acatccttgg aaaacgccaa
     1381 ggatgttttt ttattattta agatagccaa gataatcaaa tttttcaaag gtgtggtcat
     1441 tagcgatggc cacaatgata gtattcggtt ctaaaggttc aaaaggtttg acattagtat
     1501 ctaaggtttt cacttccttt tgacgcattc caataatgtt caattcatac ttacgacgaa
     1561 catctaactc tgaaagagac tgtccttccc aacttttagg aataataaac tcaatcatgg
     1621 aaatgccatg ttcgaggtag atgatgcttt cgatatgacg tcttaacaga tttgaagcta
     1681 cacgttttcc agaatcacgt tcaggggtga tgactttagt tgctccgatg ccatatagaa
     1741 cttcttcaaa aattttattt ttagccttgg caataatggt tggaacaccg agttttttac
     1801 agtgcatgac tgcaagaaca gaagattcca agttattccc tgaagcgata acgacagtat
     1861 cacaatgttc aatccctaca gctaatagaa attctttgtc agtaatatct ccaacagcgg
     1921 ctttagtgac caagtcggcc acctctttaa catgactttc tctgatatca atagcaataa
     1981 cgtcttgatc aaaattactt aattctctag caacagtacg gccaaaaata ccaaggccaa
     2041 gaacgccgac agttttacgt tttaacatgt tttctcctta acccactaaa atatcagtag
     2101 tggcatattg aatggttttt tcttttcgtt gaatcagact gatgaggaca gtaattgggc
     2161 caacgcggcc gacgaacatc aagacaatga caataagacg tccagcagta gacaattgtg
     2221 gtgtcaaatc catagatacc ccaactgtcg cgatggcaga aatagattca aataagagtg
     2281 gtataggagc gatatgaggc tccacgctaa gtaataaaat aaaacctatc ataagaactg
     2341 caaaaaagaa gataagaact gtcatggtct gcttaattgt cttatttgcg ataattcggt
     2401 tacgaaaggt aacttctgat tgccctgaaa gttccgcttt aaagagtaag aaagtaatgg
     2461 cagcagtcgt cactttaatc ccaccagctg ttccaccagg agcaccacca ataaccattt
     2521 gaatcatata aagaatgtta gttggtgcta gggtatcgtt ataagagatt gttgcaaagc
     2581 cagctgttct catagtgact gtttgaaaaa atgaaaccat aagttgctga tgtaaactaa
     2641 aattggcaat ggttttgctg ttatcttttt ctaaaaacca ggttaagaag gttccaagaa
     2701 aaagaataac agctgtggtt tgtagtacta gacgagactg attggataat ttacgaaaag
     2761 ttgccccata acaatgaggt ctttcaaaaa agtatttttt aaaagctact cctaaatcaa
     2821 cccaaaccgc aaaaccaagt ccaccagaga taatcaagaa cgtaatgata acatttaatg
     2881 taggatttaa cataaagtct tttaaactag aagatcctaa attgtcaaaa ccagcgttac
     2941 aaaatgccga cacagctaga aaaatactat tgaagatacc atttttccac ccgaagcggg
     3001 ggataaagtc aatcatgata actagcgcag caaaggcttc tagactaaag gttactttat
     3061 aagcaaaaaa aaggtagtgc tttaaatctt tgctatcacc tcgatttaac gctgattgga
     3121 gcaaggtttg atcgctcaat cgcatttttc gcttgagggc aaaagtactc acagctatta
     3181 acgtcaccaa gccaagacca ccaatctgca ttagtgccat agcaatggtt tgtccgatac
     3241 cgttatacac ttcagcgacg ggaacaacag aaagtccagt cacacaaacc atggaaacca
     3301 cattaaagaa atgatcaaga tagacggtat taggaccatt ttgataatgg gtaaagggca
     3361 tggataacaa gagagtccca atcagaatca caatggcaaa actaaatgtt aaacgctgtg
     3421 tgactgaaag agatttgata aaagagcgtt tcatgtttcc tccagacata atatctccta
     3481 ctataataac aaaaataagt taaaaaagct aagtttcatc ttagcctttg gtactattta
     3541 tagaggtttt ttgttaggca agccagcttt tcttggatac ttgttaggtg tttccttttt
     3601 cttttcaaca atggtgatga agcgagaatc tccatttggc aattggtagc tatggttttt
     3661 aatgacttta ccaaaaagaa gacaaagagc attcttagct tcttctaact cttggtcagc
     3721 agcttgtgcc tttaaggcga ttaattttcc tccaattttt aaaaagggga tggttaattc
     3781 agatagaacc tgcatccgag caaccgctct ggcagtaacg acatcaaatt gacctcgaaa
     3841 ggccttgtcc tgtccgaaat cttctgctct accatgaaaa aaatggacat tttccaagcc
     3901 caattcctga gcaaggagtg tcagaaaaga aatccgctta ttaagagaat caataatggt
     3961 aacctcaagg tttgggaaaa gaattttcat gggtaggcta ggaaaaccag ctccagctcc
     4021 aatgtccaat aatttgattg gttcattagc taaaaagccc tgtaaaatag gagcgatgga
     4081 atcataaaaa tgcttgaggt aaacttcatt tttttctgta atagctgtta aattaatttt
     4141 agtgttccat tccactagta atttgaagta ggtgtcaaat tgctcttttt gcttcgatgc
     4201 taaggaaaaa ccatcttcct ctagtgttcg gtaaaagtct tggggtgtca tttgtatcct
     4261 ctgtttttga tagttgttac cattttatca taaaatagcc cataacaaaa tccaataaat
     4321 catcagaaat aacacctcat tacttgctat tagcctagaa attgctataa ttgagacaca
     4381 aagattaaag gagacccact atgccaacag ttttgattat cttagtcgtt ttaggagtgc
     4441 ttgcgctatg gttaatgatt agttacaata gcctggttaa atctcggatg cacacaaaag
     4501 aagcttggag tcagattgat gtgcaattaa agcgtcgtaa tgacttgatt ccaaatctta
     4561 ttgaaaccgt aaagggatat gctagctacg agcaaaagac atttgaaaaa atcactgatt
     4621 tgcgtgcgcg tgttgcgaat gcctcaactc ctcaagaaac catggcggct tctaacgaat
     4681 tgagtaaaca agtgaccagt ttgtttgccg ttgctgaaaa ttacccagac ttaaaagcta
     4741 acgaaaactt cttgaaatta caagaagagt tgaccaatac ggaaaataaa atctcatatt
     4801 ctcgtcaact ctataattca acaacgtcta attacaacct tcaattagaa tctttcccaa
     4861 gcaatatcgc tggtaaatta tttggtttta aaccaagtga attcttacaa acaccagaag
     4921 ctgaaaaaga agttccaaaa gttgaattta acttttaaat atgaaattat catctagtag
     4981 gcaatcataa gagaaaggag aattatgctt tatcaacaga tttcacaaaa taagcaacga
     5041 acagttgttt tattagttgt tttcttcgct ctcttagcac ttattggagc gtcagcaggc
     5101 tatctgctgt tagataatta tgccatgggc ttggttcttg ctcttgtcat tggggtgatt
     5161 tatgctacca gtatgatttt tcaatccacc agtcttgtaa tgagtatgaa caatgctaga
     5221 gaagttacag aaaaagaggc tccaggcttc ttccatattg tcgaggatat ggctatggta
     5281 gctcagattc cgatgccgag agtttttatt attgaagatc cttctttaaa tgcttttgcg
     5341 acaggatcta gccctcaaaa tgctgctgtt gcagcaacga caggtttact tgaagtcatg
     5401 aatcgtgaag aacttgaagg tgttatcggc catgaaatta gccacatccg aaattatgat
     5461 atccgtattt cgactattgc tgtagctttg gcgagtgctg ttaccgttat ttctagtatc
     5521 ggtgggcgta tgctatggta tggtggcggt tctcgcaggc aaagagatga cggagatgat
     5581 gatgttttaa gaatcattac attacttttg tcgttattat ctttgctttt agcgccccta
     5641 gtagcaagtt tgattcagtt ggctatttct cgtcaaagag aatatctagc tgatgctagt
     5701 tctgttgaac taacaagaaa tccccaaggt atgattaagg ctcttgaaaa attgcagtta
     5761 tctcagccaa tgaagcatcc tgttgatgat gctagtgcgg ccttgtatat taatgagcct
     5821 cgcaaaaaaa ggagcttcag ttcattattc agcacccatc ctcctattga ggagaggatt
     5881 gaaaggttaa aaaacatgtg agcgtttggg gtttttgctc aagcctcact tttttatgtc
     5941 tttgggcaga taatctaaaa tggcaggaat tatcaaaaat atctgaacaa aaagaaaaaa
     6001 atgagtgaaa caatggttaa aaatagaatg atttaaatct taaaattaaa aaacgtcaga
     6061 aaataaatgc aaaactattg acaaatcttt tagatagtag taaaaataaa tgagagtttt
     6121 attaggagaa gatgatgtta gctatttccg aaatcagaaa acacccagac ggtctctcgt
     6181 ttgatcgttt atgtgatgtc aaaagtatgt tacttgagcg tgaccaacag attattgaca
     6241 taaaggcagt taaagctgtc ggtaatgttc gctatgataa ggggctttat ctgttagatt
     6301 atcaattgag ttacgaagtg atattaccgt ctagccgttc tatggttccg gtctgtttaa
     6361 gtgaagtcca acacattcaa gaattattta tagaggcgac agatttagct gataagaaag
     6421 agttggttga agacaatctg gtattagttt tagacaaaga cgctattaac ctcgaagaaa
     6481 gtattgtgga taatatctta cttgctattc cgctacaggt cttgacagag gaagaaaaga
     6541 aatcaaaaga gttgccagca ggtcaaaatt gggcagtttt gacagaagaa gactaccagt
     6601 gtctaaaaga agaaaaacaa aaagaaaata atccttttgc tagcttgcaa gggttgtttg
     6661 atgaataaaa ctgaatatta aagatgtctg taatttttat ttgaaaaaac agctattctt
     6721 tgatatactc tttagagaat atggttactt atctaaagta gaaggtattt tgaccataga
     6781 gggcaaagaa ggtaatgact attagatgct tcatatttta ttcgctagaa gactatttga
     6841 ccattagaac tgaaatagtc taggatatga gatggatttc atagttttct ttctgggaga
     6901 aaaagataga ttaagaggat aagggttggt ataaatgaca aagaaaattt taattattga
     6961 agatgaaaag aatctggcta gattcgtttc tcttgagctg caacatgagg gttatgaagt
     7021 cattgttgag gtcaatggtc gcgaagggtt agaaactgct ttggaaaaag agtttgattt
     7081 aatcctgctt gacttaatgt taccagagat ggatggtttt gaagtgaccc gtcgtttgca
     7141 aaccgaaaaa acaacatata tcatgatgat gactgcgcgt gattctatta tggatgtggt
     7201 tgcaggttta gaccgcggtg cagacgacta tattgttaaa ccgtttgcca ttgaagaact
     7261 acttgcccgt attcgtgcta ttttccgccg tcaagacatt gaatctgaga agaaagtgcc
     7321 tagtcaaggc atttatcgag atctagtttt aaatccacaa aaccgttcag ttaatcgtgg
     7381 cgacgatgag atttctctca ctaaacgtga atatgatttg cttaatattt tgatgactaa
     7441 catgaatcgt gtcatgacac gtgaagaatt attgtcaaat gtttggaaat atgatgaagc
     7501 cgttgagact aatgttgtag atgtctatat tcgttatctc cgcggcaaaa ttgacattcc
     7561 aggcaaggaa tcttatatcc aaacagtgcg tggcatggga tacgttattc gtgagaaata
     7621 agtcatatgg aaaatcagaa acaaaaacag aagaaatata aaaactcgtt accaaaacga
     7681 ctatctaata tcttttttgt tctttttttc tgcattttct ctgcctttac actgattgcc
     7741 tatagttcaa caaactattt cttattgaag aaagaaaagc agtcagtttt tcaagctgta
     7801 aatattgtta gagttcgtct ttctgaggtg gactctaatt ttacattaga gaacttagca
     7861 gaagttttgt acaaaaacga taaaacacat ctgagaattg atgacagaaa gggcagtcga
     7921 gtcattagga gtgagcgcga tatcacaaat actctagatg cgaatcaaga tatttatgtc
     7981 tataacattg ataaacagat gatttttacc acagataatg aagaatcatc tcctggcttg
     8041 catggtccta tcggtcgggt gtatcacgac catatcgaag atcagtatcg tgggttttcc
     8101 atgacacaaa aggtatattc taatcggact ggaaaatttg tgggctatgt tcaagtcttt
     8161 catgatttag gcaattatta tgtcattaga gcaagactgc tgttttggct actagttgtt
     8221 gagttatttg gcacaagctt agcctattta atcatcttaa ttactacgcg gcgctttttg
     8281 aagcctttac acaatttaca tgaagtaatg cgtaatatct ctgaaaatcc taataattta
     8341 aacctgcgct cagatatttc gtcaggagat gaaatcgaag aattgtctgt tatctttgac
     8401 aatatgttag acaaattgga gacacatact aagttgcaat cacgttttat tagtgatgtc
     8461 agccatgaat tacgaacacc ggttgcgatt attaaagggc atattggtct cttacaacgt
     8521 tggggtaaag atgacagtga tattcttgaa gaaagtttga cagcaacagc gcatgaggct
     8581 gaccgtatgg caatcatgat taatgatatg ttagacatga ttcgcgtaca aggctcattt
     8641 gagggacatc aaaatgatat gacagttttg gaagattcta ttgaaactgt tgttggtaat
     8701 tttagagttt taagagaaga ttttatcttt acatggcagt cagaaaatcc aaaaacgata
     8761 gcccgtattt ataaaaatca ttttgagcag gctttgatga ttcttattga caatgctgtg
     8821 aagtattccc gtaaagaaaa gaaaatcgcg attaaccttt cagtgactgg caaacaagaa
     8881 gctattgtta gagttcaaga taaaggcgaa ggaatttcta aagaagatat tgaacatatc
     8941 tttgaacgct tttatagaac ggataaatca cgtaatcgaa caagtactca ggctggatta
     9001 gggattggct tgtctattct caagcaaatt gtagatgggt atcatttaca gatgaaggtt
     9061 gaaagtgaat taaatgaagg ttcagtgttt atcttacata ttcctttggc ccagtctaac
     9121 gagagttaga gtagcttaag gcattgttaa tgtgacaggt atagtatgta atcattagat
     9181 aatagtagca ggcttttgcc acaactggtt agaagctgat gctatttttt tagcaatgat
     9241 ggatgatatg aaagaaatac tgtcaatcgc gatgcctttt gttatccttg agaatatggt
     9301 aaaatagtgt attagattaa tttgatagaa ttggggtgtt tgcgtgcgtt gtccaaaatg
     9361 taattaccat aaatcgagtg ttgttgacag tagacaggct gaagacggca acaccattcg
     9421 tcgtcgaaga gaatgtgaac agtgtcatac tcgctttact acctttgagc gtgttgaaga
     9481 actcccttta ttagtcatca aaaaagatgg cacacgagag caattttcta gagataaaat
     9541 attaaatggg gttgtccaaa gtgcgcaaaa acgacctgtc tcgagcacag atattgaaaa
     9601 tgtcatttct cgtattgaac aagaagttcg aacaacctac gaaaatgaag tgtcaagtac
     9661 tgctattggt aatttagtga tggatgagtt ggccgaactc gatgaaatca catatgtacg
     9721 ctttgctagt gtctacaaga gtttcaagga cgttgatgaa atcgaagaac ttttgcaaca
     9781 aattacgaat cgcgtacgcg gaaagaaaaa acgtttaaat aatgatgaaa ccaattgata
     9841 cttttacata tctaaaacgc aacaaagtaa ctcttgattc ggtgactcta atacagttgt
     9901 actttccaat tattggaagt gacgcggtct ctatctatca atactttatt cacttttttg
     9961 acgatggttt acaacgtcat aaattcagcg atattttaaa tcacctacag tttggtatga
    10021 aacgttttga agatgctctt gccatattga cagcaatgga attagtgagt gtgtatcagc
    10081 tatcagatac atatctcatt acgttgcacc agccgcttag ccgtgacttg ttttttcaac
    10141 atccagctta ttctcgatta ttagaacaaa agattggaga ggtagctgtc tctgagttgc
    10201 aggtaactgt cccaagtcaa gcaagaaata tttctaagcg attttcggat atttttgggg
    10261 tgcaaggaga tttgacgaac gtacctcaaa agccacaaaa gaattttgac ctttcaagtt
    10321 tccagcaatt aatggttcgt gatgggttac aatttgaaga taatcaaaaa gatatcatta
    10381 gcctttacag tattgctgaa cagtatgata tgacgtggtt tgatacttat caaatagcta
    10441 aggcgacggc tgttaatggg aaaattcgac ctgaacgctt attggctaaa aaaaatcaaa
    10501 gcatgacaaa accaagcaaa gaaaattttt cacaggctga gcaaattatt ttgcgagaag
    10561 caaagcaaga cagtgcttta gtctttttgg aaaaaattaa aaaggctaga cgtgcaacga
    10621 ttactaagga tgaacgcatt ttattgcaaa cattggccaa aatgaatttt cttgatgatg
    10681 ttatcaatgt catggtgctt tataccttta ataagacaaa atctgctaat ttacagaaga
    10741 gttatgtctt aaaaatggcc aatgatttcg cctaccaaaa ggtgtcaacg gctgaagaag
    10801 ctatagtggt tttgcgagcc ttcacggatc gtcaaagtcg tcggcaatca aaagtaaaaa
    10861 caagtcaaag taatgtgcct aagtggagca atcctgatta ccaagagaca acaagtcaag
    10921 aagagcaggc gaaactagat cagttcaaac aggctgcttt gaagaggtta gaaaacctag
    10981 gaaaaggggg tgactagatg gaaaagattg gagaaaccat ggcgaaattg ggtcaaaaca
    11041 ctcgtgttaa tagcgaccag ttgattcaaa ccattttagc ggatcctgaa gtagcaagct
    11101 tcatcagtca acatcactta tctcaagagc aaattaacct tagcttatct aagttcaatc
    11161 aatttttggt ggaacgccag aagtatcaac tcaaagatcc ctcttatatt gctaagggct
    11221 atcagcctat tctagctatg aatgagggct acgctgatgt gtcttatttg gaaaccaaag
    11281 aattggtaga agctcaaaaa caggcagcta tctctgaacg gattcaactg gttagtttgc
    11341 caaaatccta ccgccatatt catttatcag atattgatgt taataatgcg agccgtatgg
    11401 aagccttctc tgctatttta gattttgtgg aacaataccc atcagcagag caaaaaggct
    11461 tatatttgta cggagatatg ggaattggca agtcttactt gttggctgct atggcccatg
    11521 aattgtctga gaaaaaaggt gtgtcaacga cccttctaca ttttccgagc tttgccattg
    11581 atgtaaaaaa tgccatttca aacggctctg ttaaagaaga aatcgatgcg gtcaaaaatg
    11641 taccagtgct cattttagat gatattggtg cagagcaagc cacttcttgg gtccgtgatg
    11701 aggtgttgca ggtgatttta caataccgta tgttagagga attgccaacc ttctttacct
    11761 ctaattaccg ctttgcggat ttggagcgaa aatgggcaac catcaaagga agtgacgaaa
    11821 cttggcaggc caaacgtgtc atggaacgcg tgcgttattt ggctagagag tttcatttag
    11881 aaggggctaa ccgccgttaa gtttgagata gcaatcaaga agaatagcta cacgtatgag
    11941 tgagtgaaag aaaaggagaa tcatggtttt acctaccgtt gccattgtgg gccgtccaaa
    12001 tgtgggcaag tccaccttat ttaatcgaat cgcaggagag cgcatttcaa ttgtagaaga
    12061 cgttgaaggg gttactcgtg accgtattta tgctactggg gagtggctta accgccaatt
    12121 ctcattgatt gatacaggtg gtattgatga cgttgatgcc ccgtttatgg agcaaatcaa
    12181 gcaccaggct caaattgcta tggaagaagc ggacgttatt gtctttgtcg tttctggtaa
    12241 agaaggggtg accgacgctg acgaatatgt gtctaaaatt ctgtatcgaa ccaatacccc
    12301 agtcatttta gctgttaata aggttgataa tcctgaaatg cgtaatgaca tttatgattt
    12361 ttattcactt ggtttagggg atccctatcc agtttcttct gttcatggta tcggaacagg
    12421 ggatgtcttg gatgcgatcg ttgaaaactt gccagtagaa gaggctgaag aaaatgatga
    12481 tattattcgc ttcagtttaa tcggtcgtcc taatgttggg aaatctagct tgattaacgc
    12541 tattttaggt gaggaccgtg ttatcgctag ccctgtcgca gggacaacac gtgacgctat
    12601 tgacacgcat tttacggatg ctgacggtca agaatttacc atgattgaca cggctggaat
    12661 gcgcaaatct ggcaagattt acgaaaatac agagaaatat tctgtcatgc gtgccatgcg
    12721 tgccattgac cgctccgatg tggtcttgat ggttatcaat gctgaagaag gtatccgtga
    12781 atacgacaag cgtatcgctg gctttgcaca cgaagctggt aaaggaatga ttatcgtggt
    12841 taacaaatgg gatacccttg acaaggataa tcataccgtt gctaagtggg aagccgatat
    12901 tcgtgaccaa tttcaatttt tgacatacgc tccgattatt tttgtgtcag ctttgacgaa
    12961 gcagcgcctc aacaaattgc ctgacttgat taaacgtatc agtgagagtc agaataaacg
    13021 tatcccatct gctgtcctca atgatgttat catggatgct attgccatta atccaacacc
    13081 aacggataag ggtaaacgtt tgaaaatttt ctatgccact caagtgtcag taaagccacc
    13141 aacatttgtc gtctttgtta acgaggaaga attgatgcac ttctcttatc tacgcttctt
    13201 ggaaaaccag attcgcgcag cctttacctt tgaagggaca ccgattcatc tgattgctcg
    13261 caaacggaaa taagaaatga acatcaacta aaatgctgac ctaagaaata atctcatcat
    13321 tgataaggac tgtgttgtgt atgttacagg agacagtcct ttttataata cctttaaggg
    13381 tgtagacgga agattagtag caaacaaaga aaaaggattt tgacaagaaa tatcagtcgg
    13441 tttttgctat aatgaaagga taagcaaaaa ggtggaaaaa tgatggctag attaattcca
    13501 ggacgagtaa ggaatgaagg tattaagctt tatgagcaag gtttagtctc attccaagat
    13561 gataataaag gcattcttca aatagaagtg gagacttatc aggttcaata tggagcagat
    13621 gatgaggata tcacttgtca atgcgacaca tttcatatga aacattattg caagcatatt
    13681 gcagcagtcg agtatttttt aaaaaatgat caaaaaggta agcttttttt aaaacagtta
    13741 acaaatcaaa caaaaatcaa agaaacaaca aagaaaatga catcttttgg tagcctcttt
    13801 ttagatagct tagccatgaa tgaagatgat tctgttaaat acaggctgtc tgctcttggt
    13861 agtcgaagcc atttttcgtc agattactgg tggagtctga aaatcaatcg tttaccagat
    13921 gatcgttctt atgtgattcg agatattaaa ggctttttgc agcttattaa aaaagaaggc
    13981 ttttatcaga ttggaaaaaa ttattttgaa caactttcat ggctgcagtt tgacccatcc
    14041 agtcaagacc ttattgagtt tttatggcgc ttagcatctg atactgacaa aggggacaat
    14101 gagaacattt ttccaaatca tgcaaggcat ttgcgcttac ctagtggttt ttttgaagaa
    14161 ggcattcact atttgacgtc actttatgac tttacttttg aggggccttc tcaaacttat
    14221 catcatcttt ttgtccgttc tttggaagca gaggcaggcc tttatgagtt taaagtggaa
    14281 gtgcatcgca aatctattga attgcaaatt gctgaaaaaa atgtgcaata cctctttgat
    14341 aatgactatc ttctttatca agataccttt tatcacttga cgttaaagca acgaaaaatg
    14401 gttcaggcta ttcgtagtct gcccattgag gccgacttgg ctaaacatat tcattttgat
    14461 ttggatgatc atgcgaaatt agctgcaagc ttatcagatt ttaaacagat cggtctagta
    14521 gaggctccta aaagctttgc tattagagac tttgaggtga cttttcaatt tgatttgtta
    14581 aatcgagatg aaatcagttg tcagcttatg tttgattatg gcaattacca agttagtgac
    14641 aaagcaagtt tagaagcact tccttttgct agtcatttaa aaaaagaaga aaaaatcaat
    14701 cgttctttac ttgcttttgg cttctcgcca caattttact caaaaaagcg actaacaagt
    14761 gcaaaagagc tctatacttt ttttgaagaa acagttccat gttttgagcg cttaggtaat
    14821 gttgcgttga gtacagctat ccaagcatta caagtgaaag aaatgcccaa gatcgctatt
    14881 aaacgcaatc aggggttact agacatctcg tttgattttt caaccattat tgaaaatgat
    14941 attgaccaag ctgttacagc tctttttcaa aataatcctt attttgtgag tcaaacaggt
    15001 caattagtgg tttttgatga tgaaactcaa aaagttagca aaagcttaca agaattaaga
    15061 gcaagacagc taaaaaatgg tcacttgcaa ctggatggca ttagagctct acaagtatcc
    15121 aaactttttg aaggcatgac atctgttcat ttttcaaagg aattagaaga gttagcctat
    15181 catttgcagc atcctgaaac tttttcaata aaaccgttac cagttaaggc tcaaatgcga
    15241 gattatcaac gcaacggtgt tcagtggtta tcaatgctca atcattatgg ctttggtggc
    15301 attttagcag atgacatggg acttggaaaa actctccaga cacttgcttt tttagctagt
    15361 cacctgaaat cagatagtaa agtgctcatt ttatctccat ctagtcttat ctataattgg
    15421 tttgatgaat gtcaaaaatt cacacctcag ctagatgttg tggtttctta cggacttaaa
    15481 caaaccagag atcaaatcat cgaagaaggt catcaaataa ccataaccag ctattcctcc
    15541 tttagacagg attttgagac ctatcaagcg tttcactacg attatcttat cttagacgaa
    15601 gctcaagtga taaaaaatgc gcaaactaag atttcacact gtttacgtgc gtttaataca
    15661 gccaattgtt ttgcgctttc gggcactcct attgaaaata aaatgcttga aatttggtcc
    15721 attttccaaa ttgttttgcc agggttgcta ccgactaaaa aagagttttt aaaattaaca
    15781 gctgagcaag tatcacgcta tatcaaaccc tttgtgatgc gtcgtaaaaa agaagatgtc
    15841 ctaccagaat tacctgattt aattgaaact aattattcaa acgagatgac agatgaacaa
    15901 aaagctattt acttagcgca gttaaggcag atgcaagatc aaattcgaaa ttctagtgat
    15961 gtggatatta gccgtcaaaa aattgagatt ttatctggta tcactcgatt gaggcaaatc
    16021 tgtgacactc caagtctctt tatgaattac caaggcaaaa gtggtaagct agatagtttg
    16081 cgaattttat tgacacaaat taaagaaaat ggtcatcggg ctttgatttt ttctcaattt
    16141 agagggatgc tggacttggc caagcaagag atgactgctc taggattgac ctcttatcaa
    16201 atgacgggat ctacccctgc caatgaacgt caagaaatga ctagagcctt taataacggt
    16261 tccaaggatg cttttttgat ttccctcaag gcaggtggag ttggaatcaa ccttacggga
    16321 gcagacacag tgattttaat cgatttatgg tggaatcctg ctgttgaaat gcaggcaatc
    16381 agtcgtgcct accgtattgg tcaaaaagaa aacgttgaag tatatcgctt aatcactcga
    16441 gggacgattg aagaaaagat tttagagctt caagaaagta agcgcaattt ggtgactaca
    16501 gttttggatg gcaatgaaag ccgagcaagt atgtccattg aagaaataaa agaaattttg
    16561 gggctaaaca ctcattaatc ttccttgatt ttagtgacat taaaaaccct ttaaagaaga
    16621 tgacaccaaa agcaccttat ataacctcgt taaaacgctt cttttgaaat atctttattg
    16681 atatagcgat tgttatttgc tagtaatgat taaaacattt agaaaaagac aagtcataga
    16741 atcatcaagg aatgctttga aaaataacag tatttaagtt ataatagagg atgctacaag
    16801 aagggagaat caaaaaagga tggtaaaaaa tcagtttcct ttagttgcag atggcattgc
    16861 tattagtgat ccagcaaagc aaatggctct gtatgaaaat gaagatctca ttaccaacat
    16921 ccgtggctat taccaggaca aggagtatga tgatattgct ggaaatgagg agcttactgc
    16981 taaagcaact tctcggcagg ccccttctag taagcgcttt tgtagcaatg atgaaaaaca
    17041 ccattatgtc aaagaggcgc gtcaaaaagc aaagcaagat ctcaaggaaa aaagacaggc
    17101 ttatcttgcc aaagaaatgg cctatgttcc taaacaggtt tctaaaaagc aacagcctgc
    17161 ggattcttct ccttctcaaa agcaagcaac aacagaaatg agtcgtttta caaaaaagct
    17221 tcatcaagac aactatattt tagctgagtt gccaaaagaa tataaagagc caaaaaatct
    17281 tcctcaacaa ggaactacaa aaaaaaataa ttacgatttt ttaaaatcca gtcaaattta
    17341 caacaataaa gaaatgagac aacaacgaga aaaaacaatt gcgcaagagc ttaatcttag
    17401 taggtttgag gatcttcctt aataaatcta ctcagcgtat gcagatgaca aaggagttaa
    17461 gatgtcaaaa acgtatcatt ttatcggaat taaagggtca ggaatgagtg ccttggccct
    17521 tatgttgcat caaatggggc ataaggtgca aggtagtgat gttgaaaaat actatttcac
    17581 tcaaagagga ttggaacaag cgggcattac tatcttacct tttagcgagg acaacattac
    17641 gccagatatg gaacttatcg ttgggaatgc ctttcgtgaa aacaacaaag aagtggccta
    17701 tgctttgaga caccagattc ctttcaaacg ttatcatgac tttttaggtg attttatgaa
    17761 atcatttatc agttttgcag tagcaggtgc tcatggtaaa acttctacaa cgggtttatt
    17821 gtcacatgtc ttgaaaaata tcacagacac atcttattta attggtgatg gtactgggcg
    17881 tgggtctgct aatgcgcagt attttgtttt tgaatcagac gaatacgaac gccattttat
    17941 gccttatcac ccagaatact ctatcattac taatattgat tttgaccatc ctgattattt
    18001 cacaggaatt gctgatgtgc gaaacgcttt caacgattat gctaagcagg tcaagaaagc
    18061 tttgtttgtt tatggtgaag atgacgaact aaaaaagatc gaagcacctg cacctattta
    18121 ctattatggt tttgaagagg gcaatgattt tatcgcttac gacattacgc gtactacgaa
    18181 cggatcggac tttaaggtaa aacaccaagg agaggtcatt ggtcagtttc acgtacctgc
    18241 ttatggaaaa cataacattt taaacgctac agcagtaatt gcaaacttgt ttgtagccgg
    18301 cattgatatg gctctagtgg cagaccatct taaaactttt tcaggtgtga aacgtcgttt
    18361 tacagagaaa attatcaatg acactattat tattgatgac tttgctcatc atccaaccga
    18421 aattgtggca acaattgatg cggctagaca aaaataccca agtaaagaaa ttgtagcaat
    18481 attccagcca catactttta ctcgcactat tgccttatta gaggattttg cttgtgcttt
    18541 aaatgaagct gacagtgttt atttagccca gatttatggt tctgcacgtg aagtagataa
    18601 aggtgaagtt aaggttgaag atttggctgc taagattatc aaaccatcac aagtagtaac
    18661 ggtggaaaat gtctctcctc ttcttgatca cgataatgct gtttatgttt ttatgggagc
    18721 tggagatatt cagctatatg aacattcttt tgaggaatta cttgcaaatt taactaaaaa
    18781 taaccaataa catgtcctgc cttaggcagg ttttctcatt ctaaaggagt ttgatatgtt
    18841 gattagacag gttcaaggat ccgatttgga agttattgct accattgaaa gtgacaattt
    18901 ttcaccacaa gaagcaacga caagagcagt tcttgaagag cgcatacggc tgataccaga
    18961 tactttttta gtggcactta tcgatcagga aattgttggc tatatcgaag gtccagttgt
    19021 cactactcct attttggagg attctttgtt ccacggggtg actaaaaatc ctaaaactgg
    19081 aggctacatt gctattacta gtttgtccat agctaaacat tttcagcaac aaggggttgg
    19141 gacagctttg ttggcggcac taaaagattt agtagtagcg cagcaaagaa caggtcttat
    19201 tttgacttgt catgattact tgatttccta ttatgaaatg aatgggttta ttaaccaagg
    19261 aatttcagag tctcaacatg gtggaaccct ttggtaccaa atgatttgga aactttagaa
    19321 tcacagctga ttcaactgtt ggtttgttaa aaataagtaa caaatgcatc atttaattgc
    19381 aattaggata cttttaagtt ataatggcgg ttgattatat ttaggaggat tggctttgac
    19441 cgattttaag gataaagacc aacaagatca gcagcgaagc tttaaagagc agattcttgc
    19501 tgagttggaa aaagcaaatc aaatcagaaa agaaaaagag gaagaacttt ttcaaaaaga
    19561 gttggaagct aaagaagcag ctaggagaac ggcccagctg tatgctgaat ataaaagaca
    19621 agatactttt caaaaagagt ctatagcaca taacaataag acagccaaac actttcaagc
    19681 tataaaagat gcggtaatga cttcagaagc gattaaaccg actttacttt ctgaaaaaga
    19741 aaactcatct ttaaaaacga caagtaagag agtcgtgcag gcaaatgagc ttcaagagac
    19801 tgcctctaaa gaatctcaag taccgttaac tattgagaaa ggtcattcag tgagacgaaa
    19861 attaagcaaa cgccaacaga ctgagcgagc tgctaaaaag atttcaaccg ttttgattag
    19921 ctctattatt ataacccttt tggctgttac tctagcagga gcaggctatg tttatagtgc
    19981 tttaaatcct gttgataaaa atagtgatgc ctttgttcaa gttgagatac catctgggtc
    20041 aggcaataaa ttgattggtc aaattcttca aaaaaaaggt ttaatcaaga atagcactgt
    20101 ttttagtttt tatacaaaat ttaaaaactt tacaaatttt cagagcgggt attacaatct
    20161 gcaaaaaagt atgagtctag aagaaattgc tagtgcttta caagaaggtg gtacagcaga
    20221 acctaccaag ccatctcttg ggaagatctt gattccagaa ggatacacga ttaaacaaat
    20281 agctaaagct gttgagcata atagcaaggg aaagaccaaa aaagctaaaa caccttttaa
    20341 cgagaaggat tttttggatt tagtcacgga tgaggctttt attcaagata tggtaaaaag
    20401 atatccaaaa ttattagcaa ctatcccaac taaagaaaaa gctatttacc gtttggaagg
    20461 ttaccttttc ccagcaacct ataactatta caaagaaact accatgagag aacttgtaga
    20521 ggacatgctg gcagctatgg atgctacttt ggtaccctat tatgataaaa ttgctgctag
    20581 tggtaagaca gtcaacgagg tattgacctt ggcctctttg gttgaaaaag aaggttcaac
    20641 agacgatgac agacgtcaaa ttgcaagtgt cttttataac cgccttaata gcggaatggc
    20701 actacaatct aatatagcta ttttgtatgc gatggggaaa cttggtgaga aaacaacctt
    20761 ggctgaggat gctactattg acaccaccat taattctcct tataatattt ataccaatac
    20821 aggtctgatg cctggtccag ttgctagctc gggggtttct gcaattgaag caaccctaaa
    20881 tccagcctca acggattatt tatactttgt ggccaatgtc catactggtg aagtttacta
    20941 tgcaaaaaca tttgaagagc actctgcaaa tgttgaaaaa tatgtgaata gtcaaattca
    21001 gtaaacacaa acatggtgct taagcaccat gtttgtgttt ctgattcgtt taaaaaagaa
    21061 aagagagaaa acaattatgg cagaaaaaac gtatccgatg accctaacgg aaaaagaaca
    21121 acttgaaaaa gaacttgaag aattaaaact tgtgcgtcgt ccagaaatag tggagcgtat
    21181 taaaattgca cgttcgtatg gagatctttc agagaactct gaatacgatg ccgcaaaaga
    21241 tgagcaagct tttgttgaag gtcaaatttc aacgcttgaa actaagattc gctatgctga
    21301 aatcattgac agtgatgctg ttgcaaaaga tgaagtcgct atcggaaaaa cagttattgt
    21361 tcacgaagtt ggaacaacag ataaagatac ctatcatatt gtaggtgctg ctggagcaga
    21421 tatcttctca ggaaaaattt caaatgaaag cccgattgct caagctttga ttggtaaaaa
    21481 aacaggtgac aaggttagaa ttgaatcacc agcagctact tatgatgttg aaatcatcag
    21541 cgttgaaaag acaagttaat cagatgaagc cattatttgc ctaaccacta agtgttttgg
    21601 ttgctaatgg cttttctagt atcttcaaat gaggttgact gttaggaagg tcaagtatta
    21661 atcgcattga ttatgagcca agaggactat agatcaaaga tcgttttcga tagttattta
    21721 ataccaaaag aaaagaccag caccttaatg tactgacccc aaaaagttgg acactatatt
    21781 ttaagtatag gaagtgtata atctaaggaa actgacacta atgattgacc gttttgcaag
    21841 actttattaa tgatttcttg ctttatttta aatgaataag tctgatacgt ttccttcctt
    21901 aacactatcg actccataat gacccatcag tcaaacggta tatctcaaac gactacggtt
    21961 tactccaaat tgattggcta gttgaggcca agaaacacct gattttttta atgctaaatt
    22021 tctagcttat cttcatagct taatttcata caaaaacacc ccaatcgtta gatttttttg
    22081 atatgtcccc tttcactaga tgagtccagt aaaagggggc atatcgtttt ttgtctaact
    22141 tttggagtgc agttcataat tgtgctaggt tttttgtctt tcttaacgct ttcgctgttt
    22201 tccagcatta cgattggatt ttattttttt aggcaagtta gcctgttcca tgttttgtga
    22261 aggcgtaaca tccttacgag aagaagttga ttggtatgct ttaggcgggg ttttagcgta
    22321 ttcttcttta atttgcttat gcaatcgagg ttttaagagg taggttgtga ttaactgttg
    22381 aataatacta aagaaaccac caactagcca gtaaagacca acaccagcag ggagtgagaa
    22441 ggacatgaag atcatcatga taggcatggt gtacatcatc gtcttcattt gctcacgttg
    22501 ctcttctgaa acggccatca ttgataacca tgattggaag aagtaaagag cagcgataat
    22561 tgctgttaac actaaactac ggctaccaag atcaatgccc ataaaggtac ttgttgatac
    22621 tcctttagtg tattgagcgg caaaatacat ggcagaaaag aatggcatct ggatgagaag
    22681 aggtagacat ccaatacctc caagagggtt aatcccatga gcacgctgag cagccatcaa
    22741 ttctgtttgg gcagccattt tttcttcctg actacttgcc tgtttgatac gtttgttgat
    22801 tggttcaaaa acaggtttca agaaggtcat tttctcagat tgataacttg ctttccaaga
    22861 ttgatagaga cctagtggta agataagtgt tctaacaata atggttacga tgatgatagc
    22921 cagtccataa cctagtccag cattgtttgc aaagtaatcg ataaagtatg acatgggttt
    22981 tccaagaaat tcccaaatca tacctttggg atttccgtga gcgtctcttc ccacacagcc
    23041 tgtcaaggtg aatagaattg acagtgctag tcctgaaaag aggatgcgat ttaatgttag
    23101 tctcaatgtg tttatccttt aattttaata tacttatcta ttttacagtt tttttcagaa
    23161 attcacaata gttctgaagg ccgattttta attgcctatt tgaaagtcct gaaagtcttc
    23221 aaaattagcc atggtgacat ccacataagt gacttttgcc catttggatg gtcctttacg
    23281 aacttcttga ataaatgtgg ctattttatc tgaatctttt gattgggcca gaatttcaac
    23341 ggtcccatcg ttgttattcc agacacgacc ataaatatct cctatatcta gagctaaagt
    23401 atgggtagcg tatcgaaaac caacaccttg tacacgtcca gaaacaatta aacgtacttt
    23461 ttgcataaga agggcctcct tgcttttatt ataacacata cttagcagga aatctcttta
    23521 gctaattgtc tacttcctgt cacttttact aaagacaaga atacaaaaat gagatagtta
    23581 gtagtactaa cagtaaaaga ggtttgtcta ttttaaaaat agtatcaaaa aaaggtcttt
    23641 taggtttaaa acattgaact gcctccaaat tgaccatcta agaagttgat gctttcaaat
    23701 gctgtcgcgt atggtataat aggcgcatgg ttattacatc aaaagcaaat caactaatta
    23761 agcaaacaaa aaaattattg caaaaaaagc atcgaaaaca gtcctatttg attgagggct
    23821 ggcacctttt tgaagaagcc caaaaatcag gacaagtttt tcgccacatc tttgttttag
    23881 aggaaatggt ggagcgtcta gcaggtgagc aagaactcgt tattgttagc ccacaagtgc
    23941 tcaaggagtt gactgactct cctagtccac aaggcattgt tgctgaggtg gagataccta
    24001 agttagcctt tccatcagat tacaaaggga aatatttggt tttagaggac gtccaagacc
    24061 caggaaatct tgggaccatc attcgtacag cagacgctgc acggtttgat ggggtctttc
    24121 tttctgaaaa atcagcagat atttacaacc aaaaaaccct acgttccatg caaggtagcc
    24181 attttcattt accaatttgg cgaacagatg tctatcagct ttgtcgagag ctccaagagt
    24241 ataaaactcc catcctagca acaactctgt caaagaaatc tgttgactat aaaagcctca
    24301 cacatcatga aaggttggct ttggtgttgg gaaatgaagg ccagggcatt agtgctgaaa
    24361 tggcagccct agcagaccaa ctggttcata ttacaatgcc tggtcaggcg gaaagtttga
    24421 atgttgccgt agcagcagga attctcattt tcagcttgat ttaagctaat atgatataat
    24481 aaccaagtga ggtaattatg ttatgcatta tacagaagat aaagaatata tggaacacgt
    24541 cggccatttg atcgctcatc ctcgctttca aaaattgagt catattgttc agcaccaaca
    24601 ttcaacgcgt cttgaacatt ctatcaatgt ttcttattca agttataaat tagccaagcg
    24661 atttggttgg gatgctaaaa gtacagccag agggggctta cttcatgatt tcttttatta
    24721 tgattggcgc gtgaccaagt ttaataaggg tcatgcctgg gttcatcctc gtattgctgt
    24781 acgaaatgcg aaaaagttaa cagaactcaa taaaaaagaa gaggatatca ttctaaaaca
    24841 catgtgggga gcgaccattg cttttccacg ttacaaggaa agttatattg tgacaatggt
    24901 agataaatac tgggctgtca aggaagcagt gacgcctttg agacaaaaat ggtctaatcg
    24961 acggtttctt cgccgcaaga ccctccaaag tcataatcgt taagtaaagg agttattatg
    25021 aacgatcacg ttatttatac acaatcagat gtgggattaa atcagttttt tgctaaaatt
    25081 tatagtctag tgggaatggg agtgggtttg tctgcttttg tttcttattt gatgctttat
    25141 ccatttagag aaaatttaat ctctattctt gttaatcagc cgatgattta ctatggtgct
    25201 gcgattattg aacttatttt agtttttata gccagtggtg cagcacgaaa aaacacacca
    25261 gctgcattgc ccatttttct tatctattct gcgctaaatg gctttaccct aagctttatt
    25321 attgtggcat acgctcaaac gacagttttt caagcatttc tttcttcagc agctgtgttt
    25381 tttgccatgt caatcattgg tgtcaagact aaacgggata tgtcaggtct tagaaaggct
    25441 atgtttgctg ctttgattgg tgttgttgtg gctagtctga ttaatctttt tattggtagt
    25501 gggatgatga gttatgtcat tagtgtgatt tccgtgctaa ttttctcagg tctcatcgca
    25561 tcagataatc aaatgattaa gcgcgtttat caagcaacaa atggccaggt tggtgatggc
    25621 tgggctgttg ctatggcctt aagcttgtat ttagatttta ttaacttatt tatcagcctt
    25681 cttcgtattt ttggaagaaa tgattaacat taaaaagtga gtataactac tcacttttta
    25741 atgttaacag gataagtaaa ctgtaaggaa gaaattcgag atagatataa gatgaggctg
    25801 ctaaaagacc tagtgttaat ttgtaaactc tttct
//
LOCUS       DAUELZ010000014        22540 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.014, whole genome shotgun sequence.
ACCESSION   DAUELZ010000014 DAUELZ010000000
VERSION     DAUELZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22540
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.014"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(48..1775)
                     /locus_tag="VHI09_000454"
     CDS             complement(48..1775)
                     /locus_tag="VHI09_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120357.1"
                     /translation="MIKTDHHLLKRMLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVVIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQMVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLTPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEILRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRDEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHHLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            complement(1772..3496)
                     /locus_tag="VHI09_000455"
     CDS             complement(1772..3496)
                     /locus_tag="VHI09_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120358.1"
                     /translation="MKRLRPYVKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLLSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLDPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KQQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEAPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSIPISDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGEQK"
     gene            complement(3493..4104)
                     /locus_tag="VHI09_000456"
     CDS             complement(3493..4104)
                     /locus_tag="VHI09_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEQ6120359.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLVYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIEHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLFRKLS"
     gene            complement(4105..5082)
                     /locus_tag="VHI09_000457"
     CDS             complement(4105..5082)
                     /locus_tag="VHI09_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEQ6120360.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKEVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            complement(5089..6345)
                     /locus_tag="VHI09_000458"
     CDS             complement(5089..6345)
                     /locus_tag="VHI09_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEQ6120361.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTETLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     PYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALDPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMSVKESQTIARLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            complement(6335..6786)
                     /locus_tag="VHI09_000459"
                     /pseudo
     CDS             complement(6335..6786)
                     /locus_tag="VHI09_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(6804..7394)
                     /locus_tag="VHI09_000460"
     CDS             complement(6804..7394)
                     /locus_tag="VHI09_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEQ6120362.1"
                     /translation="MEYLASIIELGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITAALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGECAVILRQGAITKEELLANVPELRF"
     gene            complement(7378..8217)
                     /gene="prmC"
                     /locus_tag="VHI09_000461"
     CDS             complement(7378..8217)
                     /gene="prmC"
                     /locus_tag="VHI09_000461"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEQ6120363.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            complement(8217..9296)
                     /gene="prfA"
                     /locus_tag="VHI09_000462"
     CDS             complement(8217..9296)
                     /gene="prfA"
                     /locus_tag="VHI09_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEQ6120364.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            complement(9331..9900)
                     /locus_tag="VHI09_000463"
     CDS             complement(9331..9900)
                     /locus_tag="VHI09_000463"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEQ6120365.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            10038..10223
                     /locus_tag="VHI09_000464"
     CDS             10038..10223
                     /locus_tag="VHI09_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEQ6120366.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            10276..11214
                     /locus_tag="VHI09_000465"
     CDS             10276..11214
                     /locus_tag="VHI09_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEQ6120367.1"
                     /translation="MFVTQQLKLMGTVIDIQIKSDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQAAGVKPVSVHSDLFNLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFE
                     DAIVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNALVHGPNPKSPDKTFRIGVQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTAKGKQYHHILDRQTGYPIETDMLSLTVMASSSLDCDIWATRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(11278..12561)
                     /locus_tag="VHI09_000466"
     CDS             complement(11278..12561)
                     /locus_tag="VHI09_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEQ6120368.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSSAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSVMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIH
                     VPTPFYFGMPTFDITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFAS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(12561..13142)
                     /locus_tag="VHI09_000467"
     CDS             complement(12561..13142)
                     /locus_tag="VHI09_000467"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEQ6120369.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     regulatory      complement(13229..13324)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(13447..14430)
                     /gene="guaC"
                     /locus_tag="VHI09_000468"
     CDS             complement(13447..14430)
                     /gene="guaC"
                     /locus_tag="VHI09_000468"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEQ6120370.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAEQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKAYEY
                     EFVTSLKEDTPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVNGETFKEYYGSASAYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(14708..16234)
                     /locus_tag="VHI09_000469"
     CDS             complement(14708..16234)
                     /locus_tag="VHI09_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEQ6120371.1"
                     /translation="MPSLFVTFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIIISFGITLL
                     ALLASYTPMALSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDIYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
     gene            complement(16227..16955)
                     /locus_tag="VHI09_000470"
     CDS             complement(16227..16955)
                     /locus_tag="VHI09_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120372.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPDM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVANLFGGDEHA"
     gene            17378..18067
                     /locus_tag="VHI09_000471"
     CDS             17378..18067
                     /locus_tag="VHI09_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEQ6120373.1"
                     /translation="MKNAISILALLTPENQLEDTFIRELSHHFSHLFEVTILTSKANI
                     ESNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTRF
                     YQKFDRHFLEYLPLQARLSDAKGNILVDNHAFNGSFLPTTDNEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLSQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            complement(18260..19015)
                     /locus_tag="VHI09_000472"
     CDS             complement(18260..19015)
                     /locus_tag="VHI09_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEQ6120374.1"
                     /translation="MGNIALVTGASAGFGRAIVEHLIADGIKVIGASRRLEKLKALET
                     SFGSDNFYPLQMDVTDTKAIDDALTSLPNQWQDITILVNNAGLALGLDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDDGIIINLGSTAGTIPYPGANIYGASKAFVKQF
                     SLNLRADLAGSHIRVTNIEPGLCEGTEFSKVRFNGDEARVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVTRD"
     gene            complement(19142..20137)
                     /gene="pta"
                     /locus_tag="VHI09_000473"
     CDS             complement(19142..20137)
                     /gene="pta"
                     /locus_tag="VHI09_000473"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEQ6120375.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFIFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            complement(20141..21046)
                     /locus_tag="VHI09_000474"
     CDS             complement(20141..21046)
                     /locus_tag="VHI09_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ6120376.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            complement(21043..21879)
                     /locus_tag="VHI09_000475"
     CDS             complement(21043..21879)
                     /locus_tag="VHI09_000475"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEQ6120377.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEI
                     DS"
     gene            complement(21854..22525)
                     /locus_tag="VHI09_000476"
     CDS             complement(21854..22525)
                     /locus_tag="VHI09_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ6120378.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
BASE COUNT         7007 a         4711 c         3902 g         6920 t
ORIGIN      
        1 taactttagg aagacttaaa aagttcacct ttgttaaaag acgaacttta ttctatcttt
       61 tgtaatctag cataacatcc gttttttgac attaagctgg catgtgttcc ccactcaacc
      121 aatcgacctt gatccataac aagaatcaaa tcagctgatt gaatcgttga taaatgatgg
      181 gcaatgataa agctggtcct tcctctcatg agttctttta gtgcctcttg aataacagct
      241 tctgttctaa tatcaataga ggaggtggct tcatctagga tcaagacttt tggttttttc
      301 aaaaacattc tggcaatagc taataactgg agctgccctt gggacaaagc atcatcagaa
      361 gcacttaagt aagtatcata agtcttagga agttgcataa taaagaagtg tgcatgagct
      421 gctttggctg ctgccactac ttcatcacgg ctagcctctt cactgccgta agcaatcaac
      481 tcatgaacag tcgcatcttt caaccaggtt tcttgcaata ccatgccagt aatggaccta
      541 agttcttcct tagcataaca cttaatagga actttatcta acttgatgct accagcatca
      601 agttcataaa agcgcatgag taaattgatt aaagttgatt tgcctgcacc tgtaggccct
      661 acaatcgcta ccttggaacc cgcaggaaca gaaaaattaa tatccttcag taagggcctg
      721 tctttttgat aagaaaaact tacttcttca aagtcaattt gccccttaac cgctgatgcc
      781 ttaaatgtta gaaagtgctc ttgctctttt atttcaatat ctaaaaggtc ataaagcctt
      841 tgcgcacaag ctaaagaact ctgtatctct gccaagacag atgaaatatc gttgaaaggt
      901 ttggtatatt ggacaacaac atttaaaaaa gtggttagct gacctactga aaaaagtcca
      961 gccatgatac gaacagcccc taaacctgcc agtaaagcat aaatgacact attaataaag
     1021 cgagtggctg gattgactgt tgaggcgtaa aaaatggctt tttgtgaagc ctcgcaatag
     1081 gttttattta aaacatgata atcacagata gactgctctt gagcattaaa cagttgaacc
     1141 aaaccttctt gacgaaggat ttcttcagta aactgagcta aatttcctct gcttttggtc
     1201 tgtgcttgtg cataatgaaa actcttttga gcaataaaac gagctaaaaa gagggagctt
     1261 ggtgttaaca ccaaaaccaa gattaacatg agccagtcaa tctgtgccat agctataatg
     1321 gtacacagaa tcgtcaatag tccaagtata aactggttaa aaaccatttg tagaccattg
     1381 atcagttgct cagtgtcggt tatcactcga ctaaccaaat ctccaatagt ttgacgatct
     1441 agatatgcaa aaggaagacg atgaattttt tccaaaagct tatctttcaa ttgctggcta
     1501 tattgatata gcaaacgact atagacaaga ggcatcaccc attgaatcaa tgtgttgacc
     1561 acaataacaa ctaacatttg ccccaacagc cattttaaag tttgccaaga attgactgac
     1621 aaactcatat caacggcttt accgataaga acaggcaagt aaacacttaa accaacttgt
     1681 acaaaactag ctatcacgag aatacaaaca ggtagaggtt tttttaacaa atcctgaagc
     1741 attcgcttta ggaggtgatg atctgtttta atcatttttg ctcccccttc acgtaaagcg
     1801 ctagcatttc ttgataaagt gcattgctta cttttaactg gtcatgactg gcaaaatcca
     1861 attgatggcc ctgttccaac actaaaatct tatcagcaaa ctgcaaattc ttaatggatt
     1921 gcgtgactag aatcactaaa acatctgata actcttcttt tagagcttta aaaagtcgtc
     1981 ctcgagtctc attgtctaag gcggaactgg catcatccaa aatcaaaatg ggttttggct
     2041 tcagaagtgc tctggcaata gctagacgtt gccgttgccc tcctgaaaaa tgacggccga
     2101 aggcttcaac aggtgcctct aattgttctg gtaaggcagc gacaaactct tttgcttgag
     2161 ccaattcaag agcccgccac agttcctcat cactaatagg tattgattgt cccagtagga
     2221 ggttacttct aatcgttcct ttaaataatt gagctttttg aggaacaaga gcaatcacat
     2281 ttcgccactc ggttagtgtt tttggcactt ctccttgcca attaagacta atctcaccac
     2341 tatattgtga ataagtttgg cagatcaagt caatcaaagt cgttttgcct gctccagtac
     2401 caccaatgat gccaatccat tctccctgat cagcacttaa ctgaatatca tacagacttg
     2461 gctctgctgc tcctggataa gtaaaggtca agtgccggat ggtcaacaga tgagttgaaa
     2521 taggaccatc tttaagtaag ggcaaaggcg cttcatcagc taaaacaaac acctgattaa
     2581 tccgttttgc ggctgttaca ctttgattga tggttcccat caacatggtc attttcaaaa
     2641 gttctgccaa aatctgcaag aggtaattaa ttaaagcaac taacatgccc tgactaagcg
     2701 atctatgggc tacttgccaa cttccttgcc aaatcaagat taacagggta agattgacca
     2761 ctaaaaaagt cataggattc aagacattgg ctagggtagc agcttgatat tggtggcgac
     2821 ttaagagcat attttgctgc ttaaatgctt ggagttcttt ttgggtttga ttaaaagcct
     2881 taattactcg gattccttgt aactgttggc tagtcaaacg aaccaagtga tctgtttctc
     2941 ggcggattaa gagataaagg ggatctaata aatgtgacat aacaaaaaca agtgttagta
     3001 agacaatcac catcatcaca aaccataggg ttagacttgg actaatccaa taagccatta
     3061 ccatagcacc acatacgata ataggggccc ttagaaaaag acgcaaaaat tgattgatac
     3121 cagtttgaat ttgaaaacta tcactggtca atcgtgataa cagactagca taaccaagat
     3181 ggtcttggtc ctccttgctc aaaaacatga tttttttaaa caaatcatct gtcatttgtc
     3241 ttgtaaaacc aacagctgct tttgaagaaa aatactgggc tgtaacggaa agcaataagc
     3301 caatggttgc taaaccaaat aatgttaaaa caaccctcaa aattccctgg ctgttgtgtt
     3361 gactaatcga tatatcaatc atgttagcaa ttaacaaagg gactaataat tcaaataaag
     3421 cttctaataa tttaaaaaga ggacctaaga tactttcttt taggtaccct ttcacatatg
     3481 gacgtaatcg tttcatgata actttctaaa aaggctcatc agttcaatta aataaagatt
     3541 gaaatgaact tctcgtgagt aatagatatt gccgccattc ttataaagct tacctccaga
     3601 aatcaaggcg agggggtggt aataaaaata ggtttgacca ctagtgttgc ctaggcttgg
     3661 cgccacaacg gttttggaat aggtagtcgc taaatcaacg gtattttgac ctccatgttt
     3721 agcaatataa tcaatataag ctgttccaaa attataggcc tgaacagccg tccaagaatc
     3781 tactcctgct tcctcagcta gagcaagatt atgagacagt aaattcaccc cgtgctcaat
     3841 actggcctga ctatctgtaa tcgagttttt ttgtcctgaa ctgctttcac tggattgcat
     3901 gacatctgct tctcctcctt ttgtctcggt gtatatcata gctaagacta agtctacatt
     3961 tgcctttgta tcattttctg ctaatgtttt ctcaaccata ggcttatata ccaaaacacg
     4021 ttgaacattg tgatgaataa caaaggattg ataaatgaca aaaagaagaa gaaaactgca
     4081 ggcacgcttt aaaagtctaa acatctattt ggttttaata tcctctatat ttttaatcaa
     4141 gattgaataa gtcccatcat catttaacaa aaattcaacc gcttcagcat cctgataaat
     4201 ggcatttggt actgttaatt ctatcccgtt tgataacgat agcttttgat tttctagttt
     4261 ttttatctgt ctagaatgat caatatcgct aatttttata ggttctggaa tgacttcttt
     4321 gacttgatcc acaaaggtta aacgggctgt taaattatca tcaaagagtt ggtcagctaa
     4381 tttttctggt gataaaacgt cgtctgcttc taactgctta aaaagagtgg atttcatctt
     4441 tgactgaaaa gtaaaatcat cttgattaaa atgctctgca attttttgag cggtttgttc
     4501 tatcattttg atagactttt taacagattg ctcaggtgta actttcaaga ggtgttctga
     4561 aaaataatta gcaaaactgc cattatgctt cacacgcttt tcaatcaagt agtactgacc
     4621 agatttgaga ttaatgacta gtgcttcatc tggtgtctgg gtgggactcg gcagattatt
     4681 ctgcgtcacc gtaaatggat gttcataatt gtcagaaagg tgagcaaact gttctttcaa
     4741 agcaattcgc aagaaagcaa aaaagggttc gccatcctta tcaaactgga caaagactaa
     4801 atcattggtt ttttgatcct ctgaaataac aaaggcttcc ttccataact gggcaatggt
     4861 aacagacgtt tccaacaaat catcaccaat ggttgtaaaa aaggtattat tagcctcaaa
     4921 ctgaccccgt ttggcttcat ccgagaacac tttagccaat tttttccgga aatattcatc
     4981 aattcgtggt gtaatgctca ctaaacgatc tgataacagt aactcagtgt cattgggaga
     5041 aaattgatga atgacaatac gttttatata actatctaac atgattcctt actttttata
     5101 gagtgggaaa gcatcggtta actgacgcac ttcctgacgg acttcttcaa gaatagtttc
     5161 ttgatcatga ttaaccaagg ctttgatgat aagacgggca atggtctgac tctctttaac
     5221 actcatacca cggctggtta tggcagcaca accaatgcga atacctgagg ttttaaatgg
     5281 cgacaaggtt tcaaaaggaa tggcattttt attaagggta atattaacct catccagaag
     5341 attttgcgct agtttaccat tagcaataac cttagtgaca tctactaaaa agacatgatt
     5401 atctgtacca cctgagatca gacggaaacg atcatcttgt gcaaaaactg ctgccatagc
     5461 cgcagtatta tcaataatag cttgtgcata gtctttaaag gctggatcca aggcttcttt
     5521 gaaggcaacc gctttggcag caatcacgtg ttccagaggt cctccttgta aaccagggaa
     5581 aacggccgaa ttaatctttt tagcaagagc ttcgtcattg gttaatatca agcctccacg
     5641 aggaccacgt aaggttttat gggttgttga tgtcacaata tgcgcataag ggacaggact
     5701 cgggtgcaca cctgctgcta caaggccagc gatgtgagcc atgtccacca ttaagtaagc
     5761 acctacgtga tcagcaatgg cacgaaactt ttcaaaatca atgcttctag agtaggccga
     5821 agcccctgca acgataagct ttggctggac cgcctttgct tgttctaaaa tcgcctcata
     5881 atttaacgtt tcagtatcag catctactga gtaacccaca aaatggtagg tttttccaga
     5941 aaaatttact ggagagccat gcgtgagatg accacctgca gccaaatcca ttcctaatac
     6001 ggtgtcacca gcctcaatca aggccatata agctgctgca ttggcttgac ttccggaatg
     6061 ggcttgaaca ttggcaaatg cggcaccgaa aagtttctta gcacgttcaa tagcaagcgt
     6121 ttcgacaata tccacacatt ctgttccacc ataatagcga ttacctggat agccttctgc
     6181 atacttattg gttaaaacag aaccttgagc tgccatgaca gcttttgaaa ccatgttttc
     6241 agatgcaatc aattcgatat gatgttcttg tctttcttct tcagcatgaa tggcatccca
     6301 aagctcttta tcaaagtctt caacattacc tttatcaaaa atcattgtca tctccttgta
     6361 agtttttgat gaatcgtaat tgtgttttat catctgcata gtcgaggttt cggtaaaaag
     6421 catgtgcttc tttacgatga ctggcagaat tgaggcgaat aaaggcaatg ccttcctgct
     6481 tagcttggga ttccagggcc cttagtaagg cactcccaat tccccttctc tgataggctg
     6541 gtaaaacagc caagcccaag agattagacc atcagaagca taaagggtct cataacgttc
     6601 agcgtgaaca tagcctaata attgattggt atccttgtcg gcataagcta aaaaatagtg
     6661 atggcaatcc tcaatcaatc gttcatattg cctttctaac aagtcaagcg aaactaaata
     6721 gccaagggct ttggcgttaa tttcttgtag cataggacag tccgttttac ttaatggtct
     6781 caacattcca agcggctcct ctcttaaaag cgaagttcag gcacgttggc taataattct
     6841 tctttggtga tagcaccttg acgtaaaatc acagcacact cacctgataa gtctaagatg
     6901 gttgaatcct tacctgttag aaaaggatca tctgcataac caaacacttg aaagtcaaaa
     6961 tcttgcataa tatgatcaaa aactcgacca ctagctttgc cagataagtt tgcagaagga
     7021 ccaattaaag ggcctgtttt ttgaattaaa gctgcggtaa ttggatgact gggtaagcga
     7081 aaaccaaccg ttgaaagacc cgaattaatc caataaggaa cctggtcatt agcttttaaa
     7141 ataatcgtta acggacctgg taaaaaggct tggtataatt tcttgaggta tctcggctgt
     7201 tcttttgaaa aggctaagat actattaaaa tcagccacat taaggttcat tgctttatct
     7261 cggggtcttt gttttaagtc ataaacagca tttactgctt tttcatctaa agccttggcg
     7321 aaaagaccat aaaccgtttc tgttggcaaa acaagggcat cacctaattc aatgatacta
     7381 gctagatact ccataatcaa caaccaccat cctttctttg ccaaatatat ccgttacgac
     7441 tcgtatatgt ctttgaggaa aataggcttg taacatatct tttatacctt cagcttgctt
     7501 ataaccaatt tcaaagtaaa gcttcccctc cttagttaag taattgtcag cttgttcaat
     7561 aatcttacgg taaatagcat agccattttc tttagcaaaa agtgccaaat gaggctctga
     7621 ttgcaaaaca tttagactga cttcttcctt atcctcgtag gatatgtagg gaggatttga
     7681 cacaataata tcaaaggttc ctgaaatgag actaaaaaca tctgattcaa taaacgttat
     7741 atcaagctgg taagcatcag cattagcttt tgccaagtca agagcagctc tagaaatatc
     7801 agatgccgtt acttgccagt taggcctttc ttttttgaga gaaatagcaa tcgctccact
     7861 acctgtacca atatctaaaa cattcaacgg tgcatcaaga ttttcagcta aaatcatgtc
     7921 aactaattct tctgtttctg gccttggaat taatacacgc ttgtcgactg caagcttcaa
     7981 atcacgaaaa taagcattac ctgtaatata ttgaggagat aaatgctgac ttagagaaca
     8041 gaaaatatgt tccaaaagaa cagcatcttc aggagtcaca gcttgatttt ggtgaatcaa
     8101 catatctaga cttgaccatt ctttgatttc tctaaaaaca taggcgagat tttcgcgatc
     8161 ctcatctatt tgttctagtt tatcttcata agttctgatt aatgtagcat agttcattag
     8221 ttattgagag attctaattt ctttgtctga tcatacataa cgagtgcatc aataacttca
     8281 tccatttttc cagataaaat agtatctaat ttttgaagtg tcaaaccaat acgatgatct
     8341 gttacacgat tttgtgggaa attataagta cgaatacgtt ctgaacgatc accagtacca
     8401 actgttgatt tacgttcagc atcttgttca tcttgagcaa tttgcgcaaa atggtcagca
     8461 acacgagcac gaatgatttt catcgcttta tcacggtttt tttgctgagt acgttcttct
     8521 tgcatttcaa ctttgattcc tgtaggaatg tgtaccatac gaactgcagt agcaacttta
     8581 ttgacattct gaccaccagc accagacgcg tggtaaatat cgacgcgaag gtctttttga
     8641 tcaatgtcgt attctacttc ttcaacttct ggcattacca aaacagttgc agttgacgta
     8701 tgaacacgac cctgactctc tgtcaccggg acacgttgaa cacgatgagc tcctgattca
     8761 tatttaagct tagaataaac tgactgacca gaaaccatgg caaccacttc tttaatacca
     8821 ccaacaccat taacggatga ttccataact tcaaaacgcc atccttgagt ttcagcatat
     8881 ttttgataca ttgttaaaag atcaccagca aaaagcgctg cttcatcgcc accagcagca
     8941 ccacgaattt ctagaataat gtttttatca tcgttaggat ctttaggtaa tagaaggatt
     9001 tttaattttt cttcatactc ttctttagct gcttttgatt ctttaagttc ttctttagcc
     9061 atttcttcta attcaggatc accactggca tctttgatca tttcttcagc atctgaaatg
     9121 gtttgaatga cttgtttata ttctctataa gctgtgacag tttctcgagt attagcttct
     9181 tcacgtgata attccataaa tcgctttgta tctgaaacaa catctggatc actaagtaac
     9241 tcacctaatt cttcataacg atcttcaact gcctgcaatt gatcataaat attcattttt
     9301 ctttgtatct cctttaaaac cgattacctt ttaaatctct ggattaaaat aatgttttcg
     9361 acaaactggg atatatgttt cattaccacc aatttgtatc tgatcacctt catagacagg
     9421 ttttccattt tctgtcctta aaaccatggt tgctttctta ctacagtatt ggcaaattgt
     9481 tttgatttca tcaatcttat cggccaataa tagcaaatgt tttgaacctt caaaaagatt
     9541 gttttggaaa tcatttttca aaccaaaagc catgacagga acatttagct catccaccac
     9601 acgcgccaaa tcataaacat tttgcttgct taaaaattga ctttcgtcaa tcaggacaca
     9661 ataaggtttt tcttccaatt gggcaataaa tgtgaaaata tccatgtcat ttgaaatggg
     9721 aatagcttct cgtcgcatac caatacggct tgagacgatg ccaaaaccat cacgtgtatc
     9781 aagagcactt gtcataatga caacaggttt gccttgttct tcatagttat gagcaacttt
     9841 taaaatttca atggttttac cagaattcat tgtcccatat ttataataga gttgagccaa
     9901 agcggaaaat cctactttct atagtattct acctatttta ccacaaaggc tgaagttttc
     9961 ccagccattg aaaacagtgt gtactttttc acagcattat gttacaatga tgcccaataa
    10021 cataaaggag taacattatg ccatttgtaa caattgactt gtttgaggga cgctcacaag
    10081 aacaaaaaaa tcaattagct cgcgaggtta ccgaagtagt atcacgcatt gcaaaagcac
    10141 ctaaagaaaa tatccatgtt tttatcaacg atatgcctga aggaacttac tatcctcaag
    10201 gtgaaatgaa acaaaaatca taatgacctt cccccactcc ttagtggggg tattctgcta
    10261 aaaagaggac taattatgtt tgttacgcaa caattaaagc taatgggaac agttattgat
    10321 attcagatca aatctgacaa agcttgccaa caactaagta gagtcattga cttattatat
    10381 acttacaaaa atcgtttttc agctaatgat tcaaactctg aattgatggc tattaatcag
    10441 gcggctggtg ttaaacccgt cagcgtccac tctgatttat ttaacctaat ccaaattggt
    10501 aaagcacata gcttgtcaac accaagtaat cttaatatcg ccattgggcc acttgttcaa
    10561 gcctggcgaa ttggatttga agatgctata gtgcctagtc ataaccttat ctcacaacag
    10621 ttagctctaa cagatcctag acaggttctt atagatgaca aaaaacagac tgtcttttta
    10681 caacaagtag gaatggcttt agacttagga gccttagcaa aaggttatat tactgataag
    10741 attatggcct acctgattga agatggtatt gactctgccc ttattaattt aggtggaaac
    10801 gccttagttc atggacctaa tccgaaaagc cctgacaaga cttttcgaat tggcgttcaa
    10861 aaaccagatg ctaaacgtgg gcaacatctt ggtgtcatca aggttaacaa tcactctgtt
    10921 gtgacttctg gaatttatga aaggcagttt acagctaaag ggaaacagta tcatcatatc
    10981 ttagatcgtc agacaggcta ccctatagaa acagatatgc ttagcttaac ggtcatggcc
    11041 tcttcttctt tggattgtga catatgggct acccgtttat ttggtcttga tagctcaatg
    11101 attatcacac ttttaaacac ctttgataat attgaaggtc ttctagtaac aagaaagcac
    11161 catgtcctta tgtcaaatgg tttgagacac tattttcaac catactatca ttaaaagggg
    11221 ttaacggttt gctcaaaact gatcacagaa aaaacggctc aattagccgt tttttaatta
    11281 ttcatcttgt ttgtctttgc catttaaaac taaatttaag acaacagatg tcaaagtggc
    11341 aatcacaata ccattcgtta agaacatttg agctgtctct ggtaagctgg caaaaagatt
    11401 agtgccatta aagcctaaac ctgctgaaat ggaaacagca gcaataataa aattatactc
    11461 gttcttttgg aaatcaacac gattaagcat ttgcattcct tgaagagcaa ccataccaaa
    11521 gagaacaagc atggctccac caagaactgg gcttggaatc atttgtgcca ttgctccaaa
    11581 tttagggagt aggccaatga ccacaaggat acctgctgcg tagtaaatcg gacgacgggt
    11641 tttaatacct gagatttgga caagtccaac gttttgagaa aaaccagtgt aagggaaggt
    11701 attaaataac ccaccgagaa agactgcgat cccttctgag cgataaccat tgcgcaaacg
    11761 tttttcatct aattgatcat tagtcaaatc tgaaagcgct aagtagacac ctgttgattc
    11821 aaccatagaa accgttgcga tgatacacat catcacaata gaagtgatgt caaaagttgg
    11881 catgccaaaa taaaatgggg ttggcacatg aatccaagaa gcctctacaa caggagttgt
    11941 atctactaat cccatcatag ctgaaaccaa agttcccgct acaagcccaa tcaaaatgga
    12001 aatggatttc acaaaacctt tggtaaattt ttggactaaa agaataatga caatcgtcaa
    12061 caatgataac atcacgcttt gtgccgtagg ctctttgacg ttatctccca tattccccat
    12121 agcaacacct acgagactta agccaataac ggtaatgacc gatcctgtaa caattggtgg
    12181 gaagaaccga gcaatcttag aaaaaatacc tgccactaaa atgacataaa ttcctgacgc
    12241 aatgagggct ccaaacatgg cacttgaacc ttgttgcgct ccaataatgg atagaggtgc
    12301 cactgattga aaagcacatc ctaaaacaac tggtaatccc actcccgtat gcttggttaa
    12361 ctttaactgt aagaaagtag ccacaccaca catgaaaata tctgtcgaaa tcaggtaggt
    12421 caactctcta gcggaataac ctaaagcacc tgcgatcata attggtacta aaatagatcc
    12481 ggcatacatc gaaagcacat gctgtaagcc aaggacagct gattgcgagt gagaatgttc
    12541 ctgctttgtt gaatgtgtca ttatgcgtca gcctccaaga agtttagatt accattttca
    12601 aagtttttaa tacgtgctaa tgaggtcact tcaatgccca tatcttcaat caatcgacga
    12661 ccatcttgga aagatttctc aatcacaata ccaacgccga cgacttgtgc ccctgcttga
    12721 ccaataatct caatcaagcc tttggctgcc tgaccattag ctaaaaagtc atcaataatc
    12781 aaaaccttgt cttctttaga taggaattta ccagcgatag acaccgtgct cgtcacttgt
    12841 ttagtgaaag aataaacttc tgctgtcaaa atgccttctg tcatggtaat gtttttatgt
    12901 tttttcgcaa aaatcatagg aacatccatt gcttctgcag cgtatacggc tggtgcaata
    12961 cctgaagctt cgattgtaac cacttttgta atgccagcct cagcatattt ttgagcaaac
    13021 actttaccaa ttgctttcat caaccggtaa tcaacttgat gagttaaaaa attatctacc
    13081 tttagaatat tctctcctag aatattgcca tctgttaaga tgcgttcttc aagtaactgc
    13141 ataaatctcc ttttgattca aatagaaaaa gcctgccgca aagccagaaa ctataagagt
    13201 ttctcacttt gcaaggttaa aaaccaagca agcctaaaag cttacttatt gttagacatt
    13261 aaggtgtctt gtagagacgc tgcgccaatt cacagcataa ataagtatgt cattattgaa
    13321 ttcattatga taatttacta ttttaccatg tttcttattt ttttcaatac ctacagagat
    13381 atgataaaag catctccagc attaagctaa aaaattcttt actggtccta gctaactcca
    13441 tatcatttaa atggaatcac cattccaaat agaatttttg acgataacat aatcaacatg
    13501 acgcaaactg tctaaatctt taccacccgc gtaagaaatg gacgattgca aatcctgttg
    13561 catttcggtc agggtatctg ataagtgacc ttttgtaggc aataagattt ttttgccttc
    13621 tacattttta tgctcgcctt tttgatatgc tgacgccgag ccataatatt ctttaaacgt
    13681 ttcaccattc acttctactg tttttccagg actttcaata tgccctgcaa aaagcgaacc
    13741 aatcatgacc atactagctc caaagcggat ggatttagcg atatcaccat gtgtgcgaat
    13801 cccaccatca gcaatgatag gcttacgagc tgcctttgcg caccagcgca gtgctgctaa
    13861 ctgccaacca cctgtaccga aacctgtttt aactttagtg atacatactt tgcctggacc
    13921 gatgcctact ttagtagcat cagcacctgc attttcgagt tctcgtacag cttctggcgt
    13981 accaacattt ccagcaatga caaatgtttc tggtaattca gttttgatat gcttaatcat
    14041 atcaataaca ctgttagcat gaccatgagc aatatcaatg gtgataaact caggagtatc
    14101 ctctttcaaa gaagtcacaa attcatattc gtaagcttta accccaacag agatggatgc
    14161 aatcaagcct tgttcatgca tgcgtttgat aaaaggtttg cggctatctt catcaaaacg
    14221 atgcataata taaaaataac cctcttttgc caattgctca gcaattgttt catcaataat
    14281 ggtttgcata tttgctggga taactggtag cttgaactgg tattttccga gtgtcacact
    14341 tgtatctgct tgtgaacgac tagtaataat gcacttgtta ggaatcagtt gaatatcttc
    14401 ataatcaaat acagggatgt cattaaacat aagtttctcc tcttttagca cagatgaaag
    14461 aaaataagtc agttctcacc ttcttctcta agtcctctat acaaaataat cttcctatga
    14521 cctctgatga ttagaggtat ctttaggtaa aaacttacct ttcattttat agattactta
    14581 tttgtttata ttttgtcaaa tttatatgct atttttaatt ttaaagttcg gtttttgtat
    14641 ttatgaattg ttacttcttg aacttaagag acgaaaaaat gccagtccta cggaccaaca
    14701 tttctgttta tttctggatt aagcctgttt tcaacagata atctcgagcg accttattag
    14761 catctgctcc tttaacagat acctcgtagt tcatgtcctg catctctttt tcagtaattt
    14821 ttcccgctaa ttgattaaga atgcctttta attctggatg ttttgttaat aaggaagttt
    14881 tcatcaaagg agctccctga taaggtggaa acaactgttt atcatctttt aaaactttca
    14941 aatggtattt cgtaatttca gcatccgtag agtaagcatc tgttacttgg atatctcctg
    15001 attgaattgc ttgataacga agagctggct ccatcgtcgc cacagatagc tgtaatccat
    15061 attgagattg cattccctta tagccatctg ctctgtcctt aaattccaga gtaaaaccag
    15121 cctttaaagt atcagcatgc gcttttaaat cagaaatggt ttcaatctga tattccttag
    15181 ccaatttttc tggcatagca acagcatacg tattttggta agcaaatggc ttaagaaggg
    15241 tcagtttatc ttgcttagca atgccttttt tagcatcctc atagacctgc ttagggtcat
    15301 tagacaacgg tggtttgtcg cgtaaaagac ttgatgttat agttcctgta aattcaggat
    15361 agatatcaat atctccagat ttaagggctt catataagaa gctagttttc ccaaaattag
    15421 atttcaactc aacgcttata tctgattggt cttcaattaa ttccttatag agattaatca
    15481 gaatatcggg ctctgctccc aatttaccgg caataaccac tgtatctttt ccttttgaaa
    15541 actgactaag cgccatagga gtatacgatg ctagcaatgc aagtaaggtg attccaaaac
    15601 taattataat ccgtcgcaag gaagctttct caaggtactg taacaaacta ttgaaaataa
    15661 tagctagcag agctgaagaa atagccccta tcagaatcag attagcatta ttacgatcga
    15721 tccccaataa gatgaaagag ccaagtccac cggctcctat caaggaagct aaagtagctg
    15781 tgccgataat catgactgct gacgttcgca cacctgacat aatgacaggc atggcaattg
    15841 gaatctcaaa cgtttttaac cgctcccatt tggtcatccc aaaagctatt cctgcttcca
    15901 cgagacttgg gtcaatacca tttaaccctg tgatggtgtt ctgtaaaatc ggaaaaatcg
    15961 catagataac taaagctgtc actgcaggca acgttccaat tcccattaaa gggatgaaaa
    16021 gaccaagcaa agccagtgag ggaatggttt gaaaaacacc tgttacctgt aacataatgt
    16081 ctgaccaacg tttgctgcga ctgagaagag ctgctaaagg cacgcctatt agcaaggcga
    16141 tcataagaga taaaagggaa atttgcaggt gttcccccag agccgccagc cattcattaa
    16201 aacggttttg gaaagttaca aacaaactag gcatgctcat cacctccaaa taaattggcc
    16261 acaaaagcat tagcagggtg ggtcttaatc atttctgggc gatccagttg cacaatttct
    16321 ccctcattta aaatagccac gcggtctcct aatttaatag cctcatcaat atcatgtgtc
    16381 acaaatacaa tcgtcatgtc aaattccttg tgaagactca acattaactc ttgcaattgt
    16441 ttacgtgaaa taggatctaa ggctgaaaat ggttcatcca tcaatagaat tttaggatga
    16501 gaaatgatag cacgtacaat accaatacgt tgctgttcac ctcccgataa atcacttggg
    16561 taacgatcaa gataatcttt agcaggtagg cccaccttat ccaataattc ttcagttttt
    16621 tgtctaattt cttcagctga ccattgcttc atatcaggga taatcgcaat attttcagct
    16681 actgtgagat tggggaaaag agcaatttgc tgcaacacgt atccaataga tagcctcatc
    16741 tcacgtaaat ctaactcttt ctgtgggacg ttattcagta aaatatctcc agagcttggt
    16801 tcaataaggc agttaatcat ttttaaaagt gtggttttcc ccgatccgct ggggccaacc
    16861 agcacaaaaa attcacggtc attaatttga aaggtctgct cttgcaaaac ctttgtctga
    16921 ccaaaagttt tagaaacatt attaaaacgt atcattattt ctccaattca ttggttaaag
    16981 taacagcata aaagctgatg agttcattca acctgtctct ctgtcatttt ttctcaaaac
    17041 gttctgagta actacaagga atcactgcat gccctaccat ttttagacaa acgcatctac
    17101 taattaaagg gactcttagg gatcctcagt aaggtaaaaa ctaccaattc tcaaaaacat
    17161 tttctctcat tattttcttg tcgtttgttg ttgaccatta taaaaagttt cccactataa
    17221 gtcaaataat gagactattt ctccttaccc cttatagaaa aacaggtcac tttgtcagcc
    17281 tatctctcca aaattacctt atcaaaaatc ctagacttgc ctatgataga taagcagatt
    17341 agttgtcttt tctagaaaat acggctatca tacaattatg aaaaatgcca tttccatttt
    17401 agcgctctta acacctgaaa accaacttga agatacgttt attagagagc tttcccacca
    17461 cttctcacac ctttttgagg tgactattct cactagcaaa gccaacattg agtcaaatca
    17521 attgtcgact ttccaaggaa ttttcagttt ccatgagcat gacattgact tgcctacact
    17581 ctatttcaaa acctctcaac atggtcaggg atttttagtg acagaaagtg tctttgatca
    17641 agcaacagca gtcttatcgt taagccaata cctcactaga ttttaccaaa aatttgatag
    17701 acatttttta gaataccttc ctttgcaagc aaggttatct gacgcaaagg ggaacattct
    17761 agtcgataat catgctttta atggaagttt tttgccaact actgataacg aaatagaaga
    17821 ttggattctt gctgaattgc gtttatcaga taacccttgt aaaaccttcc tacttccttc
    17881 gggttcattg gatcatattt acatgcagca ttatcaagct cttaaaaatc ctcaaggtca
    17941 gctggtggga gttttggata ccgtccaaga cattaaaccg ttattaagcc agtatttaga
    18001 agaaactgga caagccattg ttggctggtc cgatgtgaca tcaggtcctt ccattttaga
    18061 tcactaaact tgattttaat agggagtcca gcctaaatca tcaacgcaca agaagcaatc
    18121 aagaacactt ggaaacatca aaaccttctt cttgactgct ttttttgatg actaaatcgg
    18181 taagtctcac ttataaagaa taatccattc cagtaaacca tttttctaaa gattattatt
    18241 cttatggact catcccttgc tagtcacgag taactggttg cggtccataa ctttgcgaca
    18301 ctggcattag ctcaatacga ttaacattaa catgaggggg ctgatgaatc acccatgata
    18361 cagtcgacgc aatatcttga ggttgaatgg catgtgctcc ttgataaatg gccttcaccc
    18421 gtgcctcatc accattaaag cgaacctttg aaaattctgt cccttcacat agaccgggtt
    18481 caatattggt cacacgtata tggctacctg ctaaatcagc tcgtaaattg agtgagaact
    18541 gcttaacaaa agccttgcta gccccataaa tattagctcc cggataggga attgtccctg
    18601 cggttgaccc taaattaata ataataccat catctttgga gaccatatgt ggtaataatt
    18661 gacgtgtgag ataaatcaac ccaacaatat tagtgttaat catcgtcatc caattctcaa
    18721 aatcggcttc ataagcctta tcaagtccca atgccaagcc tgcattattc actaaaatag
    18781 taatatcttg ccactgattt ggtagacttg tcaaagcatc atcaatcgct ttggtatctg
    18841 tgacatccat ctgcaaaggg taaaagttgt ccgacccaaa gctcgtttct aaggccttga
    18901 gtttctcaag acgacgagaa gcaccaataa cctttatccc atctgcaatt aagtgttcga
    18961 caatggctct gccaaaaccg gctgaagccc ctgttactaa ggcaatattt cccatacatt
    19021 tcctcaccct caaaaaaaac gagatggtct ggccaactcg tttttaataa tgtcaattag
    19081 tcttaggaca acttctaaca catcacttag cggttaggat cctatatcaa ggggtcttaa
    19141 gttacccttg gctttcaata gcttgtgctg ctgtaataat agctaatttg taaatatctt
    19201 cagcgcttga gccacgagat aaatcattaa caggtttgtt caatccttgt aagattggtc
    19261 caatagcatc aaacattcct aaacgttgtg caattttgta accaatattt ccagattgta
    19321 agtcagggaa aataaaagta ttagcttgac cagcaactgc tgaatctggt gctttgatag
    19381 ctgctgtttc aggaacaaag gctgcatcaa attgcaactc accatcaagt gccaaatcag
    19441 ggttcaaccc tttagcaatt tctgttgctt ctcttacctt atcaacttga ggcgctttac
    19501 cagaaccttt tgttgagaag cttaacatag caattttagg atcaatatca aagattttag
    19561 ctgtttcagc tgtgttaaca gcgatttctg ccaattcttg tgctgttggg tcaatattga
    19621 tagcacaatc agcaaacacg taacgctcac tcgtattttc gcgattcatc aagaaaacac
    19681 ctgaagttct tgaaatacct ggtttggttt taatgatttg aagggctgga cgaacagtat
    19741 cagctgttga atgaatagca ccagatacca ttccatctgc taatcccatt ttaaccaaca
    19801 taacaccaaa gtaattcaca tcacgaagca ttttatcagc atcttcaagc gttgcttttc
    19861 ctttacggat gtcaacaaaa gcttctttca ttttgtcaaa atccgcatat tcatttggat
    19921 taatgatagt atagtcttga tcagcgaaac ctaattttgt caaaaggttg cgtacctctt
    19981 ctgactgtcc aaggataatc ggttctaaga gtccttcaaa tttcaaacga gcagctgcac
    20041 ggacgacgcg ctcatcatta ccttctggaa aaacaatctt catgttttta ccaagaattt
    20101 tttctcttaa accaccgaat aaactacgaa tactcattct ttaataacct ctatcttttt
    20161 tctgtaaacc tatgataacg ctatcaaagt catctgtcaa gtgtattgca tagctacaat
    20221 cactttctgt aaatggatct ttgaaattca aataatgaca atgtaaagct tgccttgtta
    20281 ttcctaagtc caaacgacct ccgtaaaggt catcccctaa aagtggaaaa ccaatatggg
    20341 caaaatgaac tctgatttga tgggtacgtc ctgtatgcaa tttgatatcc actaaatgca
    20401 cattttctga atagcgcgcc acaaccttat agctagtctt ggcatatttg cccatagggt
    20461 caactgccct ggtaataata ctatcttttg aacgcccaat tggagcaata atatctcctt
    20521 catctggcaa cgtcccattt ccagacacca gagcaaaata acgtttctct attgagcgag
    20581 tttggagttg cttgtccaac cttgcatgag cataaccatg cttagcaaat aacatcaagc
    20641 cactcgtatc tctgtctaaa cgagtcacga tatgcacttg ttggtctagg taatgattgt
    20701 ccacataata tgccttgata aagttagcaa tggtattgga gtggatggca cttggaatac
    20761 tggcaaagcc aatgggttta ttaatcacca aaaaatgatc atcctcgtga acaatatcca
    20821 aatcaaaagg gatggcttca agcttttcaa agggctcttc gtttggaatg tcaatggtga
    20881 caacatctcc cacttgcaat aaataaatgg cattttgttc aataccattc actaatatat
    20941 taccgccctt atatttgatt ttagccaata gtcctttaga gacatcatga ctttttaaaa
    21001 gggtttttac ttttattcgt ttatctgcta caaattcaaa tctcatgagt caatctctcc
    21061 aataaaggca tccttgaccc tttcccaaaa gctcgtatga ctcggagagg aaacaaaatg
    21121 aattttctca tcgtcgataa aatactccac cttcgtcaca ttttttaact gataggtttt
    21181 attatcaatg gaaatggtat aaattcctaa tcgttttggc actaactcaa tcttatcttt
    21241 tttgggaata atgattgatg agcccaaggt tctaaagaca cggttattaa gactggaaat
    21301 ttccgtcaat tgcagcgctt caatcgtcgg atgcaagaca gcaccaccta aagatttatt
    21361 gtaggctgtg ctccctgtcg gggtcgatac tgaaatccca tcacctcgga agctttcaaa
    21421 tttgacatgg ttaataataa catctgctac catcgttttt tcaatacgct taaccgtcgc
    21481 ttcattcaaa gcacgcgctt taaccacacg accatcatct aaagtaatag caacttttaa
    21541 aataggataa gagatttgtt ctcccttgtc ttttcttaaa ttatcaatca atttatcaac
    21601 ttcaaaatcc ctataatcgg tataaaagcc aagatgaccg gtgtggattc ctacaaaacg
    21661 taccttatct aattcttttt catacatgtg aaaagcagat aagagcatcc catctccgcc
    21721 aatagaaatc acaatatccg gatttttctt tgaaagatag aaatcaggat catctttaaa
    21781 tacggagaaa agtttggagg caacgcgttt actttggtac ttaccatttg caataatagc
    21841 aactcgcttt accttacctg tataattcat ctgtgtcatc actatttcct acaccatcac
    21901 ttaaatttcg tgtcacgggg tcaaacaata attgagcctc ccgaatatct tctcgaatct
    21961 tacgcatttc ttcgtctaat tctaaggcaa ttttagcagt cacttcaaga cgctttttaa
    22021 tttcttctgg gaagtctcca ccatatttat aatttaaaga atgctcaatg gtcgcccaaa
    22081 agttcattgc caaagtacga atctgaatct ccgctaagac ctttttttga ccctcaatgg
    22141 tatcaacagg gtactcaacc accacatgat aagacctgta gccactggac ttcatattgc
    22201 gaatataatc tcgttcataa acaattgtca tgtcttgtcg ttgacgcaat agagctaaga
    22261 cttcttccac atcatcaaca aactgaacca taatacgtag tccagcaata tcttgaatat
    22321 cttgtgctat attttcttca ataacaccac gtaaaatcat tttttcttta atactttcaa
    22381 ttgacttaac acgtcccgta acaaattcaa tcggagaata acgattttgc ttacgatatt
    22441 gcttgcgaat accccgtagt tttattttta attcaccaac tgtttggata taaggatcta
    22501 aaaattcttc ccaatctaag gtcatctatt gtctcctttc
//
LOCUS       DAUELZ010000015        22194 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.015, whole genome shotgun sequence.
ACCESSION   DAUELZ010000015 DAUELZ010000000
VERSION     DAUELZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22194
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.015"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            117..1109
                     /locus_tag="VHI09_000477"
     CDS             117..1109
                     /locus_tag="VHI09_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEQ6120379.1"
                     /translation="MQDYYTPKGKQLTIHERRQIQRWKLEGRSNREIARLISKAPQTI
                     HNEIKRGLVRQQVRKGKFEAVYSAQHAQLVYQENRKRSVRPIRQRKQDREAIEHYMRQ
                     NYSPEMIVKAKGVQVPVSTIYYWIHHGKLSLGKEAMLYPRKAKQARKHASPYFKPAGK
                     SIEQRPDSINQRLEAGHYEIDTVIMTRAKNQCLLTLTDRKTRHQLIRLIPDKSAQAVN
                     EALTGILKDYTVNSITTDNRTEFSRLSDVFPASAIYYAHPYASWERGTNENHNRLILR
                     WLPKGTKTTTPKEVAAIEHWINHYPKRLFNYKSPVEMLKLANFNLKFGILLETA"
     gene            complement(1615..2001)
                     /gene="rplQ"
                     /locus_tag="VHI09_000478"
     CDS             complement(1615..2001)
                     /gene="rplQ"
                     /locus_tag="VHI09_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEQ6120380.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(2016..2954)
                     /locus_tag="VHI09_000479"
     CDS             complement(2016..2954)
                     /locus_tag="VHI09_000479"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEQ6120381.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(3000..3383)
                     /gene="rpsK"
                     /locus_tag="VHI09_000480"
     CDS             complement(3000..3383)
                     /gene="rpsK"
                     /locus_tag="VHI09_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEQ6120382.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(3401..3766)
                     /gene="rpsM"
                     /locus_tag="VHI09_000481"
     CDS             complement(3401..3766)
                     /gene="rpsM"
                     /locus_tag="VHI09_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEQ6120383.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(3784..3900)
                     /gene="rpmJ"
                     /locus_tag="VHI09_000482"
     CDS             complement(3784..3900)
                     /gene="rpmJ"
                     /locus_tag="VHI09_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEQ6120384.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(3926..4144)
                     /gene="infA"
                     /locus_tag="VHI09_000483"
     CDS             complement(3926..4144)
                     /gene="infA"
                     /locus_tag="VHI09_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEQ6120385.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(4262..4900)
                     /locus_tag="VHI09_000484"
     CDS             complement(4262..4900)
                     /locus_tag="VHI09_000484"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEQ6120386.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(5050..6354)
                     /gene="secY"
                     /locus_tag="VHI09_000485"
     CDS             complement(5050..6354)
                     /gene="secY"
                     /locus_tag="VHI09_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEQ6120387.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(6371..6811)
                     /gene="rplO"
                     /locus_tag="VHI09_000486"
     CDS             complement(6371..6811)
                     /gene="rplO"
                     /locus_tag="VHI09_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEQ6120388.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(7027..7209)
                     /gene="rpmD"
                     /locus_tag="VHI09_000487"
     CDS             complement(7027..7209)
                     /gene="rpmD"
                     /locus_tag="VHI09_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEQ6120389.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(7224..7718)
                     /gene="rpsE"
                     /locus_tag="VHI09_000488"
     CDS             complement(7224..7718)
                     /gene="rpsE"
                     /locus_tag="VHI09_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEQ6120390.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(7737..8093)
                     /gene="rplR"
                     /locus_tag="VHI09_000489"
     CDS             complement(7737..8093)
                     /gene="rplR"
                     /locus_tag="VHI09_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEQ6120391.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(8198..8734)
                     /gene="rplF"
                     /locus_tag="VHI09_000490"
     CDS             complement(8198..8734)
                     /gene="rplF"
                     /locus_tag="VHI09_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEQ6120392.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(8937..9335)
                     /gene="rpsH"
                     /locus_tag="VHI09_000491"
     CDS             complement(8937..9335)
                     /gene="rpsH"
                     /locus_tag="VHI09_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEQ6120393.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(9486..9671)
                     /locus_tag="VHI09_000492"
     CDS             complement(9486..9671)
                     /locus_tag="VHI09_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEQ6120394.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(9687..10229)
                     /gene="rplE"
                     /locus_tag="VHI09_000493"
     CDS             complement(9687..10229)
                     /gene="rplE"
                     /locus_tag="VHI09_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEQ6120395.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(10253..10558)
                     /gene="rplX"
                     /locus_tag="VHI09_000494"
     CDS             complement(10253..10558)
                     /gene="rplX"
                     /locus_tag="VHI09_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEQ6120396.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(10637..11005)
                     /gene="rplN"
                     /locus_tag="VHI09_000495"
     CDS             complement(10637..11005)
                     /gene="rplN"
                     /locus_tag="VHI09_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEQ6120397.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(11030..11290)
                     /gene="rpsQ"
                     /locus_tag="VHI09_000496"
     CDS             complement(11030..11290)
                     /gene="rpsQ"
                     /locus_tag="VHI09_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEQ6120398.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(11316..11522)
                     /locus_tag="VHI09_000497"
     CDS             complement(11316..11522)
                     /locus_tag="VHI09_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEQ6120399.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(11532..11945)
                     /gene="rplP"
                     /locus_tag="VHI09_000498"
     CDS             complement(11532..11945)
                     /gene="rplP"
                     /locus_tag="VHI09_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEQ6120400.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(11949..12602)
                     /gene="rpsC"
                     /locus_tag="VHI09_000499"
     CDS             complement(11949..12602)
                     /gene="rpsC"
                     /locus_tag="VHI09_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEQ6120401.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(12615..12959)
                     /gene="rplV"
                     /locus_tag="VHI09_000500"
     CDS             complement(12615..12959)
                     /gene="rplV"
                     /locus_tag="VHI09_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEQ6120402.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(12975..13253)
                     /gene="rpsS"
                     /locus_tag="VHI09_000501"
     CDS             complement(12975..13253)
                     /gene="rpsS"
                     /locus_tag="VHI09_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEQ6120403.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(13392..14225)
                     /gene="rplB"
                     /locus_tag="VHI09_000502"
     CDS             complement(13392..14225)
                     /gene="rplB"
                     /locus_tag="VHI09_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEQ6120404.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(14243..14539)
                     /locus_tag="VHI09_000503"
     CDS             complement(14243..14539)
                     /locus_tag="VHI09_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEQ6120405.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(14539..15162)
                     /gene="rplD"
                     /locus_tag="VHI09_000504"
     CDS             complement(14539..15162)
                     /gene="rplD"
                     /locus_tag="VHI09_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEQ6120406.1"
                     /translation="MANVNLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(15186..15812)
                     /gene="rplC"
                     /locus_tag="VHI09_000505"
     CDS             complement(15186..15812)
                     /gene="rplC"
                     /locus_tag="VHI09_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEQ6120407.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILVKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(16060..16368)
                     /gene="rpsJ"
                     /locus_tag="VHI09_000506"
     CDS             complement(16060..16368)
                     /gene="rpsJ"
                     /locus_tag="VHI09_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEQ6120408.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(16568..17857)
                     /locus_tag="VHI09_000507"
     CDS             complement(16568..17857)
                     /locus_tag="VHI09_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEQ6120409.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQV
                     SLLINVLNAIFSALSIYVWGFSLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITFH"
     gene            complement(18245..19261)
                     /gene="adhP"
                     /locus_tag="VHI09_000508"
     CDS             complement(18245..19261)
                     /gene="adhP"
                     /locus_tag="VHI09_000508"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEQ6120410.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADIVVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPGVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(19513..22155)
                     /gene="adhE"
                     /locus_tag="VHI09_000509"
                     /gene_synonym="adhC"
     CDS             complement(19513..22155)
                     /gene="adhE"
                     /locus_tag="VHI09_000509"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEQ6120411.1"
                     /translation="MTEGHNTVETTSVSVTIDALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAAQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAISTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKTADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
BASE COUNT         6451 a         4717 c         4024 g         7002 t
ORIGIN      
        1 aattttgttg gaaacgaaaa attctaaaat gaacttagcc actttttagg tatgaaaaaa
       61 gcacctcata agaggtatac ttgtagttca ccacaaacac aaggaaactc aaaaagatgc
      121 aagattatta tacaccaaaa ggcaaacaat tgacaatcca tgagcgtcgg caaattcaac
      181 gctggaagct ggaaggtcgc tctaatcgtg aaattgctcg cttaataagt aaggcccccc
      241 agaccattca taatgaaatc aagcgaggct tggttaggca acaggttcgt aaaggaaagt
      301 ttgaagccgt ttattctgct caacatgctc agttggtcta tcaagaaaat cgcaagcgtt
      361 ctgttagacc cattagacag cgtaagcaag atagggaggc tattgaacac tacatgcgtc
      421 agaactattc ccctgagatg atagttaaag ctaagggagt tcaagtccct gtatctacca
      481 tctattactg gattcatcat gggaaattaa gtcttggtaa ggaggctatg ctttatccga
      541 gaaaggctaa gcaggctaga aaacatgcca gtccttattt taaacccgca ggaaagagta
      601 ttgaacaacg gccagatagc attaaccagc gtcttgaagc tgggcattat gagattgata
      661 cagtcattat gacgagggct aaaaatcaat gcttactcac cctaacagac aggaaaacac
      721 gtcatcagct gattcgatta ataccggata agtctgctca agccgtcaat gaagctctta
      781 cggggattct aaaagactac accgtcaact ctatcacgac agacaacagg acagaatttt
      841 ctcgcttatc agatgtcttt ccagcaagtg ccatctacta tgcccatccc tatgcgtcat
      901 gggagagagg cactaacgaa aaccataatc gccttattct cagatggctc cctaaaggta
      961 ccaaaacaac aacccctaaa gaagtcgctg ctattgaaca ttggatcaat cattatccta
     1021 aacgactctt caactacaag tcgccagtag aaatgttaaa actggctaac ttcaacttga
     1081 aatttggcat tttgttggaa acggcttaaa gtgacagatt ttaaaagtaa aaaagatatt
     1141 ttcacctcca aattaaaaga ggattttaaa atacttatta ataaaatatt gaatagggga
     1201 taaaactaac aatatagttt gagcatctgt ttgcgatgca agtatttttt cggacattta
     1261 aatgagagga ttacatcgtt tatttgccca tgagaggtct ttaaacacct gcataaggtt
     1321 ttcttactct aatgacctta ttgtctctga gaaaacttta taaacgcatc agaagaggct
     1381 tctgtgttat tgttttgctc agcttaaaag cggggggtgt tcacctcata atacaacaaa
     1441 acgcctgctt ataaaaagtt tctatagctc agataccttt cataaacaag cgttacatat
     1501 taacaatatg atgagtgttc ccgctaggaa aaatcctagc ggtagaccag agctagacta
     1561 agaataatct ctcgattact ccatcatcat aacactcaac aaaattgata aaaattaaac
     1621 taattcaata attgccattg gagcagcatc tccacggcgt ggttctgttt taagaatacg
     1681 agtgtatcca ccattacgtt ctgcataacg aggtgcaatt tctgagaaaa gtttttgaag
     1741 agctgtcgtt gatgtgtatt tatcggtagc ttcatcataa ttttctgatg cgatttcgtt
     1801 acgaacgtat gcagctgctt ggcgacgagc atgaagatca ccacgtttac caagagtaat
     1861 cattttttca actgttttac ggatttcttt tgcacgtgct tctgtcgtta caattgattc
     1921 gttgatcaaa agatctgtcg tcaaatcacg aagcattgct ttacgttgtg agctagtgcg
     1981 tcctagttta cggtaagcca ttttgtcctc ctatattatt tatcgttttt tagtccgaga
     2041 cctaagtcag caagtttaac cttaacttct tcaaggctct tacgtccaag attacggact
     2101 ttcatcattt caggctcaga tttttctgtt aagtcgaaga cagtgttaat gcctgcacgt
     2161 tttaaacagt tatatgagcg tacggataaa tcaagctcct ctatggtgcg gtcaagtacc
     2221 ttttcatcgt tcactttttc agtttctttc attacttcgg tagcttttgc aacctctgtt
     2281 aaatcagtaa acaggtttaa gtgttcgatc aaaactcgag cagatagacc taaagcatct
     2341 tcaggaataa ttgttccgtt tgtcatgatt tcaatcgtta atttatcaaa gccatcattg
     2401 ctacctacac gggcaggctc aacttgataa ttaacttttt taactggcgt atagattgaa
     2461 tctactgcca atgtacccac aggtgcatca tcttttttgt ttccttctgc tggcacataa
     2521 ccacgttttt tagcaacagt cattgttgca cgtagagaat gtccttcagc gattgtaaaa
     2581 agataatgat ccgggttaac aagttcgata tcactgtcag taagaatatc accagctgtt
     2641 acctctgctg gtccttctac ttcaagttcg ataatctttt cgtcttcgac gtatgatttc
     2701 acagcaagtc ccttaacatt aaggataatt tgcatgacat cttcacgtac acctgggatt
     2761 gtatcaaatt cgtgtagtac tccatcaatt ttaattgatg ttactgctgc acctggaagt
     2821 gaagacaaga gtacacgacg aagagaatta cctagagttg ttccgtagcc acgttcaagt
     2881 ggttcgatga caaatctacc gtaatcttta ttttcatcaa tttttgttat tattggtttt
     2941 tcaaactcaa tcattttttc acccctcgaa acgaaacttg tgtactattt gttatatgat
     3001 tatacacgac gacgttttgg aggacgagca ccattatgtg gcacaggggt cacatcacga
     3061 attgcagtta cttcaagacc tgcagctgct agagcacgaa tagctgattc acgacctgaa
     3121 ccaggacctt ttacagtcac ttcaacagtt tttagtccgt gttcttgtgc agattttgca
     3181 gcagcttctg cagccatttg agcagcaaat ggagtagatt tacgagaacc tttgaaacca
     3241 agagcaccag ctgatgacca tgcaagagca ttaccatgca catctgtaat cataacaata
     3301 gtgttattaa atgtagcgtg aatgtgtgca acaccagatt cgatgttctt tttcacacga
     3361 cgtttacgtg ttggtttagc caattgtttt acctcctatt ttattttttc ttacctgcaa
     3421 tcgcaacagc tttcccttta cgagtgcgag cattgttttt agtattttgt ccacggacag
     3481 gaagtccacg acgatgacgg attccacggt atgaaccgat ttccatcaaa cgtttaatgt
     3541 tcatgtttac ttcacgtcga aggtcacctt caactttgat tgcatccact tcacgacgga
     3601 tagcatcttc ttgatctgat gttaaatctt taacacggat atcttcagaa atacctgcag
     3661 ctgctaagat ttttttagat gttgcaagac caattccgta aacataagta agtgaaatta
     3721 ctacgcgttt atcatttggg atatcaactc cagcaatacg agccattttt tctcctttct
     3781 attttatcct tgacgttgtt tgtgttttgg atttgtcgga caaattacca taacacgacc
     3841 attacggcga ataactttac agtattcgca aattggttta accgatggtc ttaccttcat
     3901 gttttaatcc ctccaattat ttcgattatt taaagcggta tgtgatgcgt ccacgtgtca
     3961 agtcataagg actcatttct actgtaacac ggtcacctac taaaatacga atgtaatttt
     4021 tgcggatttt tcctgataca gttgctagaa tttgatgtcc attttccaat tcaacagtaa
     4081 acattgcatt tggcatcgtt tctactactt tgccttcaat ttcaatcacg tcttcttttg
     4141 ccacgcaaaa gtacccccta aatttcgatt tgatgtcctc tgagcacaga ggtaacaatt
     4201 ataagtcaga ctaaactagt atatcattac taatctactt atgcaagtag attagtataa
     4261 tttatttgag ttctagcaac gctttttcaa catctgcaaa aacgtctgtt atttcttgat
     4321 taccttcaat atctgtaaca agaccaagct tacgatagtg ttcaagaata ggttctcctt
     4381 gagccatatt gacgtccaaa cgacgtttaa cagtttcagg cttatcatct tctcgttgat
     4441 agtaatcttc ttctttataa tctactggtg ggttgaacac tttatggaac gtttcaccag
     4501 ttttacgatt gataatacga ccactcaaac gctctacaag acatgatgga tccactttaa
     4561 tattaataac accatctaag cggagtccta gttcttcaag tgttgcatct aaggcgtgtg
     4621 cttgttcaat agtacgtgga tacccatcaa gcaaaaaacc tttttctgcg atatcatcct
     4681 cagctaagcg ctcttttaca atcccgtttg tgacttcatc aggaaccaat tcacctttat
     4741 caatataact tttagctaaa cgtcccattt cggtttgatt agccattgcg gcgcggaaca
     4801 tatcccctgt tgagatgtga gcaacaccaa attcttcaac aatcttagct gcttgggtcc
     4861 ctttaccagc tcccggcaaa cccatgatta aaagattcat cttttcctcc tcttttaaaa
     4921 taaatacata gtaggaaatt attttccttg gtttttgttt tcaaatggca acaagaaatc
     4981 gtttgatgat aaccaagtct tattgtcatt taaaaacaaa atagaaggtt gacaatgtca
     5041 acctgtattc tattctgctg tattcataaa tccgacatat tttctcttta gaagatatcc
     5101 ctcaagctgt ttcatacctt cgataccagt tgaaatcaaa atgagcaaac tagttccacc
     5161 taaagcaata ctagaagaaa ggttgagagc ttgttgtgct gctattggcg ctaaagagat
     5221 aaatgctaag aagatagctc ctacggttgc tagttttttt agcaatgcgg acataaattg
     5281 ttctgtctca cgtccagggc gaacacttgg tatatatgag gaattcttct gaagattttc
     5341 tgctgtcttc tcaggattaa cttgtacaaa ggtatagaag aatgagaaca atataatcaa
     5401 caaggcgtaa actatcattc cgactggagt ttgataatta aaaatttctt gcaaacggtt
     5461 taaccacggt aaatctctac cattttgaac aaaaggaata atcgtacttg ggatagttgt
     5521 aatcgagcta gcaaagataa cgggaataac gccggctgga tttactttta atggaagata
     5581 tgaacttgta ggtgcacctt gcattagctt tgtgtattgg attggaattt tatattccgc
     5641 ttgttggaca tatgttgtaa agaaaacaat agcaagaaca gctatgatta aaatcccaac
     5701 aatgagataa gacgagtgta aatcacttgc cttaacattt acaaaatagt cttcacgaat
     5761 tgttgcaatt gcacttggaa tagacgaaat aatacccgca aagataatca ttgatacgcc
     5821 attaccaaat cctttatctg tgatttgttc tccaagccag gtaacaataa cgctacctgt
     5881 tgtcagtaat gccccgatca gtaaatatgt tttgatgtct ggtgtcttaa caagtgcaac
     5941 gttcgacaaa gtattaaacc ctgcagtgat accaatggat tgggcaaaag ccaaaacaag
     6001 tgaaatatag cgcgtcgctt gatttaattt acgacgtcca acctcacctt gtttgcccca
     6061 ctcaacaaac ttaggtaaaa tatccatctg caataactga acaacgatag aagcagtaat
     6121 atatgggcta acccccattg aaaatactga gaaatttctc atcgcattac cactaactaa
     6181 gtttaacata tttaagaaag ggagttcact taattgctct aagctcttgg catttacacc
     6241 aggtacggta atatgtgtcc caattcggaa tacaagaatg ataaatatag taaagaaaat
     6301 tttattccgt acagtcttta tctttaacgc gtcttttagt atttttaaga acataatgag
     6361 tatcccctca ttagatgact tcgattgaac cacctttagc aataatagct gcttcagcag
     6421 attttgagaa cttagctgct tttacagtca attttttagt caattcaccg ttaccaagca
     6481 ctttaacgcc tgatttttca gctcggacaa taccagcatc ttttaaaata gccggagtca
     6541 cttctgtacc atcatcaaaa acattcagtt gatcaagatt cacaagtgca tattctttag
     6601 tattgatatt tgtaaagcca cgttttggaa tacgacggaa caatggtgtt tgtccacctt
     6661 caaaacctaa acgtacaccg ccaccgctac gagctttttg acctttttga ccgcgaccag
     6721 atgttttacc attacctgat gatgtcccac gaccaacacg gttacgtact ttacgtgagc
     6781 cttcagcagc ttttaattca tgaagtttca ttttttctcc ttttttttgt aaaatgctag
     6841 cgcctctatg agggaaaagg actccctata gaaactcgcc tatacattaa tgatacttaa
     6901 gttatccaaa ataacttaat acagatacgc actaaccgac tactgtttgt tgccaaaaaa
     6961 gcaaactaca agagtcggtt ttgtgctcgt ttttaattat gaaatcagag atttcacagt
     7021 tataacttat ttaacatctt caacggtaac caagtgagaa atagctgtca ccataccacg
     7081 gatagcagca ttatcttctt tgactactga agaatttaat ttaccaagtc caagagcaac
     7141 aacagtttta cgttgttctg gcttacgtcc gataggagac ttagtcaaag taattttaat
     7201 ttgagccatg atgatcccct ttcttatgct aagtcagaaa ctgagatgcc acgaagtgcg
     7261 gcaacttctt ctgcacgttt aagttgtttc aatccttcaa cagttgcacg aacaatgttg
     7321 attggagtgt ttgaaccaag agattttgaa gtaatatcag caacacctgc taattcgatg
     7381 acggcacgaa ctgcaccacc agcagcaact ccagaacctt ctacagctgg tttcaataat
     7441 actttagctc caccgaagtt agtgtaaact tcgtgaggga ttgttgtacc aaccattggt
     7501 acttcgatca tgtttttctt agcagcttca actgctttac gaatagcttc tggaacttct
     7561 tgagctttac cagtaccaaa tccaacacga ccatttccat caccaacaac tacaagagct
     7621 gcaaagcgaa gacgacgtcc acctttaaca acttttgtaa cgcggttaat cgcaacaacg
     7681 cgttcttcaa gttcaactgc attatcttta aatgccatta ttttgtgtcc tccctattag
     7741 aatttcaatc cgttttcacg agctgcatca gccaaggctt taacacgtcc gtgatagaga
     7801 tatccaccgc ggtcaaacac cacttcagaa atacctttag ccactgcacg ttcagcaaca
     7861 agtttgccga ctacaacggc ttgttctgtt tttgttcctt tagaaacgtc tttatcaaga
     7921 gttgatgcac ttgcgagcgt tacacccgct acgtcatcaa taacttgagc gtagatgcct
     7981 gtattagaac ggaatacgtt caaacgtggg cgatcagcag ttccagagag tttaccgcgg
     8041 acgcgacggt ggcgtttttg gcggattttg tttttatctg gtttcgaaat cacaattttc
     8101 acctcttaat tttaatcaat ttgtctcatt gacaagttgt cgattgaact tatcgagcat
     8161 gttaaggtgc aatacttccc atacccgact aagaacatta tttacctgtt ttaccttctt
     8221 taaggcgtac gtattcacca acgtaacgga tccctttgcc tttgtaaggc tctggtgaac
     8281 gcaagctacg gatgtaagca gctgtttgac caacaacttc tttgttgatt ccttcaactg
     8341 agattgaagt tgggttagca acagtgaaag taattccttc tggagcttca acttcgtctt
     8401 ggtgagattt acctactgaa aggacaagtt tagtaccttg aagttgagca cggtaaccga
     8461 cacccttcat ttcaagatct tttttgaaac cttcagaaac acctacaacc atgttattca
     8521 agttagcacg ggttgtacca tggattgttt tcatttcttt tgagtcgtta ggacgtacaa
     8581 ctgtgatttc agtcccttca actttgattt caatattttt gttgaactca cgagtgagtt
     8641 cgcctttagg gcctttaaca gtaataacat tgttgttatt tgttaattca acacctgcag
     8701 gcatagtaat tactttatta ccaatacgtg acatgtttgt tttctcctgt taaattgtca
     8761 agccgcatag cgactagttt tcacgggggt ttgatagaat gatatacttc aatttatatt
     8821 tcaaaattga acacgagctg tggtctgtgt gaaaaagata aatcttcctt gagtgtaaac
     8881 acgctacgtc agatttccta ttttcacttt gtccacgacg ctcttagtat cttaaattac
     8941 caaacgtaag cgatcacttc tccaccaaca ttcttttggc gagcttcttt gtcagtcaaa
     9001 agaccttctg atgttgaaat gattgcaatt ccaagtccgt taagaacttt aggcatgtca
     9061 tcacgttttg cgtaaacgcg aagacctggt tttgaaatac gttttaagtt agtgataaca
     9121 cgctcaccat tttttccgta ttttaagaac acacgaatga tgccttgttt atcatcttcg
     9181 ataacttcaa cattttttac aaaaccttca cgtttaagaa tctcagcaat ccctttttta
     9241 atgtttgaag caggtacttc taacacttcg tgttttactt gattagcatt acggatacgt
     9301 gttaaaaagt ctgcaattgg gtcagtcata accatttttt atattctcct cttactagca
     9361 gtttgatgct atcacttact agttaatgtt gtccttaaag gactttggta tcagcttatg
     9421 ctcacaccgt tttaattaag cacaagactt aatctgaaga acaaattaag ttttgtgtca
     9481 tatttttacc aagaagcttt gacaacacct ggaatttgac ctttgtaagc caattcacgg
     9541 aagcaaacac gacaaagttt aaacttgcgg taaactgaat gtggacgtcc acatttttca
     9601 cagcgagtat aagcttgtgt agagtgtttt gcaggacgtt tgttcttagc aatcatagat
     9661 tttttagcca atttatttac cccctattat tttgcaaaag gcattccaag gcccttaagc
     9721 aattcacgtg attcttcgtc agtatttgca gtagtgacga ttacaatatc aagaccacgt
     9781 actttatcaa catcatcaaa gctgatttct gggaagataa gttgttcttt cacaccgaga
     9841 gtgtagtttc cgcgtccatc aaacgatttt gttggaacgc catggaagtc acgtacacga
     9901 ggaagtgaaa cgctaaccaa tttgtctagg aattcgtaca tacgttcgcc acggagggta
     9961 acttttgcac cgatcgctac accttcacga agacggaagc cagcgattga tttcttagct
    10021 ttagtaataa gtggtttttg acctgagata agtgctaatt cagctgcagc tttctctagg
    10081 ttttttgcgt tggatacagc atcaccaaca cccatattga gaacaatttt ctcaactttt
    10141 ggcacagcca taactgatgt gtaattgaat ttctctgtca acgcaggaat tacttcgtta
    10201 gtatattttt cttttaaacg atttgccatt atgcttctcc tttccttcgt gattaatcaa
    10261 gcacttcgcc tgattttttg ctgtaacgta cttttttacc gtcaacaact ttataaccaa
    10321 cacgtccagc tacaccattt ttatctaata cttgaacatt agagacatgg ataggtgctt
    10381 ctttttcgac gatagctcct tgaggatttt cagtgttagg tttttggtgt tttttaatca
    10441 ttccaacacc ttcaacaata actttgttaa cttttggaag tgctttaaga actacagctt
    10501 cagtgccttt gtccttgcca gcaattacgc gaactttgtc gccttttttt acaaacattc
    10561 tggtttctcc tattatttct tacgccccga tgggcaccct aggaaaacct aggggactac
    10621 gtttgttaac ctgagattaa agtacttctg gtgcaagtga tacgatcttc atgtagccac
    10681 cctcacgcaa ttcgcgtgca actgggccaa agatacgagt tccgcgagga gttttatcat
    10741 cacggatgat tacagcagca ttgtcgtcaa atttgatgta tgaaccgtct ggacggcgtg
    10801 caccagtttt tgtacgaacg ataacagctt taaccacatc acctttttta actgctccac
    10861 caggagtagc ttgttttaca gaagcaacga ttacgtcacc gatgttagcg aatttacgtc
    10921 ctgaaccacc aagtactttg atagtcaaga tctcacgagc accgctatta tcagcaactt
    10981 tcaagcgagt ttcttgttga atcatttcaa ttttctcctt ttagtttgat tagataataa
    11041 cagctttttc cactacttcc acaagacgga aacgttttgt agctgaaagt gggcgagttt
    11101 ccatgatacg aacgatatcg ccttctttag caacgttgtt ttcgtcatgt gctttatatt
    11161 ttttagaata gttgatacgt ttaccataga ctgggtggtt acgtttagtt tcaactacaa
    11221 ctgtgattgt tttgtccatc ttgtcagaca ctacgcgtcc gtaaagagtt ttacgttgat
    11281 tacgttccat ttagaatttc tcctttccca atctattatt tcatttctga ttgcacagtt
    11341 ttaacacgtg caatttgttt cttaacttcg tcaaggcgag cagtcttttc aagttgacct
    11401 gctgcagctt ggaaacgaag atcaaacaat tcttttttga gttcgttttc tttcttagca
    11461 agttcttctt gagacaatcc acgaagctct ttaacaaaat ctttaatttc ttgaagtttc
    11521 atgtcttctc cttattctgc ttcacgtttt acgaatttgc acttaactgg taatttgtgg
    11581 ctagcaagac gtaatgcttc gcgagcaatt tcttcagaaa caccagcaat ttcaaacatc
    11641 actttaccac gtttaactgg tgctacccaa ccttcaggtg cccctttacc agaacccata
    11701 cgtacaccga tagcttttgc agtgtatgat ttgtgtggga agattttaat ccaaacttta
    11761 ccaccacgtt tcatgtaacg agtcatcgca atacgggcag cttcgatttg acggtttgta
    11821 atccagtggc tagttgtggc ttgaagtccg tattcaccga atgaaacttc tttaccacct
    11881 ttagcctcac cgcgcatttt tccacggaat tcgcgacggt gtttaacacg tttaggtact
    11941 aacatttgtt atttgcctcc tttagtgttt ttacgagctg gaagaacttc tccacggtaa
    12001 atccaaactt taacgccaag tttaccataa gttgtgtcag cttcttccca agcatagtcg
    12061 atatcagcgc gaagcgtgtg aagtggaaca gttccttctg aataaccttc agcacgagcg
    12121 atatcagcac cgttcaaacg accagaaact tgagttttaa tccctttagc tcctgcacgc
    12181 attgtacgtt ggattgcttg tttttgagca cgacggaaag caacacgttg ctcaagttga
    12241 cgagcaatat tttcaccaac aaggtgagca tctaaatcag gttgtttgat ttcgatgatg
    12301 ttgatgtgta cttgttttcc agttaatttg ttaagttgac cacgaagagc gtcaacattt
    12361 gcgccacctt taccgataac catacctggt ttagcagtgt gaagtgaaac aataactttg
    12421 tttactgcac gttcaatttc aatagttgaa actgatgcgt cagctaattc tttattaatg
    12481 aatttacgga ttgcaagatc ttcatgaagg taatccgcgt attctttttc agcataccat
    12541 ttcgcatccc agtcacggat gattccgaca cgcataccaa ttggatgtac tttttgaccc
    12601 acgattttac ctccttattt ttctgataca actacagtta cgtgagttgt acgtttgttg
    12661 attggtgaag ctgaaccttt cgcacgtgga cggaaacgtt tcattgttgg tccttcgtta
    12721 gcgaatgttt cagatactac caagtttgct ttttccaaac caaagttgtt ttctgcgtta
    12781 gcaattgctg agttaagagt tttctcaata acacgagctg ctttgtttgg agtgaatttt
    12841 aagattgcga ttgcgtcagc aactttctta ccacggataa gatcaagtac taaacgtgtt
    12901 ttacgaggtg aaacacgcac tgtacgagcc attgctttag ctgaagtaat ttctgccatt
    12961 gtgtcctcct cctattaacg acgtgttttc ttgtcgtcag ctgcgtgacc tttgtaagta
    13021 cgtgttggtg caaattcacc aagtttgtga cctaccatgt cttcttggat gtaaacaggt
    13081 acatgtttac gtccgtcata aactgcgatt gtatatccga tgaaacttgg gaaaatcgtt
    13141 gaacgacgtg accaagtttt gattactttt ttcttttcgt cgtttgcttg agcttcaact
    13201 tttttcatca aatgctcatc gacgaaaggt ccttttttaa gactacgtcc cattttgtag
    13261 ttttctcctt taaaaaatgt accacagcgg cttgagttca agagcaagac tccaaactct
    13321 accgagttgg cggaataaat agcaaagcta ttgtaacgct atttcagcaa gttttaagca
    13381 acttactaag attatttttc gttacgacga cgaacgataa gtttgtctga tttagctttc
    13441 ttgttacgag ttttaagacc aagcgctggt ttaccccatg gagttgatgg cgctttacgt
    13501 ccaactggtg ctttaccttc accaccaccg tgtgggtgat cgttagggtt cattacagaa
    13561 ccacgaactg ttgggcggac acctttccaa cggctacgtc ctgctttacc aatgttaaca
    13621 agtgattgtt gttcgttacc aacagtaccg atagttgcac ggcatgtacc aagaatcata
    13681 cgaacttcgc ctgattgaag acgaacaaga acgtatttac cttcttgacc aagtacttga
    13741 gcagaagctc cagctgcacg aacaagttct ccacctttac caggtttcaa ctcaatattg
    13801 tgaacaactg taccgacagg aatgtttgct aatggaagtg cgttaccaac tttgatatct
    13861 gcatctggac cagaaacaat acgttgacct acttcaagac ctttaggtgc aatgatgtaa
    13921 gctttcacac cgtcagtgta atgtacaagt gcgatgtttg cagtacggtt tggatcgtat
    13981 tcgattgttt taacaactgc ttcaacgcca tctttgttac gtttgaagtc gatcaaacgg
    14041 taatgacgtt tgtgtccacc accttggtga cgaactgtga tgcgaccatt gttgttacga
    14101 ccagctttgc tcttaagaga aacaagcaat gatttctcag gcgtgcttgt tgtgatttcc
    14161 gcgaaatcca aagaagtcat gttacgacgg ccatttgtcg ttggtttata aactttaata
    14221 cccacgttat ttcctcctta gattattcag cttcagctgc gaacaactcg attgctttag
    14281 aatcagctgt aagagtgatg atagcttttt tagtttttga agtgaaacct gtgtaacgac
    14341 caacgcgttt tgcttttggt ttaacgttaa cagtgtttac acttgcaact ttaactccgt
    14401 caaaagcagc ttcaacagct tgtttgatca aaagtttgtg tgcacgagta tcaacttcga
    14461 aagtgtattt gcctgcttca agagcaatca ttgacttctc agtgataact ggttttttga
    14521 ttacgtcgta caaattcatt atgcaagaac ctcctcaatt gtagagattg cttctttagt
    14581 aacaagaagt ttgtctgcgt tcacgatatc aagaacactt gcagttgctg cagttgcaac
    14641 agttacgttt ggaaggttac gtgcagaaag tgctgcaaat tcatttcctt cttcaacaag
    14701 aacaagtact tttgtatcaa tgctaagagc tgaaagcact tttgcaaatt cagcagtttt
    14761 tggtgctgca aatgaaaggc cttctacagc tacaaattta tcttcagcaa cttttgctga
    14821 gtaaactgat ttcaacgcaa ggcgacgaac tttttgtgga agtttgtatc cgtatgaacg
    14881 gggagttggt ccaaagacaa caccaccacc acgccattgt ggtgagcgga tagaaccttg
    14941 acgagcgcgt ccagttcctt tttgacgcca tggtttacgt ccaccacctg atactgctga
    15001 acggttttta accgcatgag taccttggcg aaggctagcg cgttggctaa ttacaacatc
    15061 aaaaacaact gattcgtttg gttcgatacc gaagatagcg tcgtttaatt caactgagct
    15121 aacttcttta ccagtttggt caaatagatt tacgtttgcc attttaactg ttttctcctt
    15181 tcttattatt tagcagcttt aactgctgac ttgatagtga taagagattt cttagcacct
    15241 ggtacgttac ctttaacaag gataacgttc ttttctggga taacttgtac aatttcaagg
    15301 ttttgaactg ttacacggtt accacccata cgtcctgcca agcgtttgtt tttgaaaaca
    15361 cggttaggcg caacaggtcc catagaacct gggcgacgat ggtaacgaga accgtgagcc
    15421 ataggaccac gtgattgacc atggcgtttg ataacacctt ggaaaccttt accttttgat
    15481 gtccctgtga cgtcaacaac atcaccagct tcaaattgtt ctacagataa ttctgcacca
    15541 acttctaagc cttcaatgtt tttgaattca cgaatgaagc gcttaggagc tgtgtttgct
    15601 tttgcaacat ggcctttggc aggtttgtta ctcaagactt cacgtttgtc atcaaaacca
    15661 acctgaactg cttcgtaacc gtctgtttca acagttttaa cttgaagcac aacgtttgga
    15721 gttgcttcaa tgacagtaac agggatgaat tcgcctgatt cagtgaagat ttgagtcatt
    15781 cccacttttt tccctaagat tccttttgtc atgagaaaat atttcctttt cttaataata
    15841 tttcaaaaag ttttttatga gcgttttttg tgctcaaaaa agttttttac gagcgttttt
    15901 tgtgctcata gcaagataca aagagcgtca aactcaaaat aaaaatagaa aatctaacac
    15961 aaaaacttct gtttcgagga agatttatct ttttcatgta gagtttagct cgtgttcaag
    16021 ttccatcaaa cactaactgt acttgcaaaa tctcaccgat taaagtttga tttctacgtt
    16081 gacaccactt ggaagatcaa gtttcatcaa agcatcaaca gttttttgtg ttggattgat
    16141 gatgtctacc aaacgtttgt gtgtacgcat ttcaaattgt tcgcgagaat ctttgtattt
    16201 gtgagtcgca cgaataattg tgtaaagact gcgttcagtt ggaagtggaa ctggtccagc
    16261 aactgtagca cctgtacgtg ttgcagtttc aacgattttt tctgccgctg tatcaagtgt
    16321 acggtgttcg tacgctttca aacggatacg gatttttttg tttgccatct tttctcctct
    16381 cgtctattta cgataatagg ctagctcctc aagaaaaccg acacctgttg cgtggcaatg
    16441 caaccgagcg tgtcgcaacc tcttgtatca ttgctaaagc tgtattttta cagcaccaca
    16501 atataataac agaatcattt agtcattgca agctttttag gcattttttt caaaaaaaac
    16561 taccttccta atgaaaggta atttcttggt actttttaaa ttgtttagag agaataaacc
    16621 accgactggt attatctaac actgtcgcca tccagacccc tatgagtcca tattgccaaa
    16681 ccacaccaat cacataacca aggccaatgc gaattaccca cattcctatg gttgttgcat
    16741 agaaaggaag ctttgctttt cccaaaccct gccagacggc tgtataaact aaagtgcctg
    16801 ccgttgcggg agctcctagt aaagaaaaaa gtaaaacaac catggcggac ctttgagcat
    16861 cagtattctg agtaaatagc ggcaagagac taggacctag cagataggtc aaggctccca
    16921 taaccagcat catgagggtg gataaaataa aagcttctct aataatatac cttatctcag
    16981 tcaccttacc gccccccagt tgcctagcca ctagaataat tgttgccgtt gccatggcta
    17041 agccaggcat gtaattaaac tgagtcaagg tttctccaat agcattacct gccaaggccg
    17101 ttgtcccaaa gcgaacaaca atgccaataa ttaggacatc tcctgctctc atcatcaaac
    17161 gttcaccagc agcaggtagt gataaatcaa aaataatttt gtccaaaggt ctcatcaatc
    17221 gtttcgcaac ctgtttaata ggaataaact gacaaagtaa aaacacaccc actaaacgag
    17281 ataaaaccgt cgcccaagca acaccaagaa gactaaatcc ccatacatag atagacaagg
    17341 cagaaaaaat ggcattgagt acgtttatta ataagctcac ttgcatagga atcttaggat
    17401 acccttgagc tcgaacaata gctcctaagc tagttaaaag tcctagacta acaatcatac
    17461 cgccgacaat ggatagatat tgccctccaa ccaatgttac agaagcttca gctcctagcc
    17521 attctaacac ctgatgatgt ccaacaactg ataacaaccc taagccgacc gaaaggagga
    17581 gtgttacttg caagacacct gccatatagt ttaattgttt gttctgatta ttttctccaa
    17641 tacttcgagc aatcagacta gatacagcag ctcccagcgc gataaagagg gactgataaa
    17701 tactgataat gttgttagct atggagacac cagagactgc tacaagtcca atttgagcca
    17761 ccagataatt atcaaccatt cccattaaca tctgtaaaat attttctatc atagaaggca
    17821 gagctaaaga gaaaattttt cttctattat atatcattta tgtcccctaa taaaaaattc
    17881 ttgatcaaac gattttttca aacacagtca ctggactcat tcagttattt tggtccatta
    17941 atgaattcat aaagtctatc ttaatagcag agtcccaaga tacacgacac ttagtgctac
    18001 agtaacaatt gaccacatgc caatccttaa catttaccta acgtttataa ctaagaagac
    18061 gaagaacaaa aggactccaa acgaattttt tctattatat aatatattct tcatttagca
    18121 actttcaatg tatcaaaata gcactctcga gtcaaaaatg gtttctgata ctcgagagcg
    18181 ctattttatt tggaatcaag tttaaacaac ttaggcttat ctcatattta atacatttgt
    18241 atgtttagct gatgaaatct aagaccttgc gcccctggat taaaccacgt tccatttcat
    18301 caaagactcc tggagcagtg tcaactggaa ctttttcgac aaccggaaca accagacctt
    18361 ctgcaccaaa ggcaaaggct tcttccaagt ctttacgtgt ccctacaagt gatccgacca
    18421 ctttgatacc atctaaaaca gtttttacga tagacagttc catgtattct gatggaagac
    18481 ctacggctac aactgtccca ccggcacgga cactatcaat tgcttggtta aaagcaactt
    18541 tagaaacagc cgttaccaca acaccatgtg ctccaccggt tttttcttga atatagcctg
    18601 gaacatcttc aatttctttg ccgttaacta caatatctgc gccaacttct ttagctaact
    18661 caagtttatc attattaatg tccactgcga caacatgtgc attaaagact tttttagcgt
    18721 attgaacagc cagatttcca aggccaccag ccccaaagat aacaatccat tggcctggag
    18781 cagcacctgc ctctttaata gctttataag ttgttacacc agcacaagta atggaagaag
    18841 cttgagccgg gtctaaacct tctggaactt taactgcata atcagctgtt actactgcat
    18901 attcactcat tcctccatca acactgtatc cagcattttt aacactacga caaagtgtct
    18961 cacgtcctgt agtacagtat tcgcaatgac cacatccttc aaagaaccat gcaatagaga
    19021 cacgatcacc aacctttaag gatgttacgc cttctccaat ttcttctact ataccaatac
    19081 cttcgtgccc caaaatacgt cctggtacct gaccaaaatc gccatgtgca acgtgtaaat
    19141 cagtatgaca aacgccacag tattctactt taacaagcgc ttcaccatga ccaacatttg
    19201 gaagatcatg ctcaacaact tcgacacctg tactagcttg attaacaact acggctttca
    19261 tatcagtcct ccatctaata atgacttatt tgtaatcggt tacaaataaa ttgtacatct
    19321 tttcacaatg aaagtcaaca gaaacctgaa aagtttctga aacttaactg gttaatatta
    19381 tagctgtttg cctatctgca aaatacttaa ctacattttc tgacactgga tataaatgat
    19441 atatttagaa aataaaatga tttcttatga ttttaatcat aagaaatcat ttcctggtat
    19501 caacattaaa aatcacttac gtcgtcctgg acgttcagca taaccatagt aagcatcttc
    19561 aataatttct tgcatgtgat caaccattgg aagacgtggg ttagcaggtg aacattggtc
    19621 ttcataagca aggtaagcca attcacgaga atgttctttc cattcgtttt cgtcgatacc
    19681 ttgagctttg aagttcattt gaataccaac tcggcaaccc agatcataaa cagctttcgc
    19741 ataagattcc actgcttctt ctggtgtaga agctggaagc cctaacaatt tcgcaatgtc
    19801 ttggtatttt tcatcagcac ggtagtaatt atacttaggc catgttgctg ttttagctgg
    19861 acgagttcca ttatagcgga taacatacgg caaaagaatt gcatttgtac gtccgtgtac
    19921 ggtatggaat tgtgcgccaa ttttatgcgc cattgagtga gaaataccta agaaagcatt
    19981 ggcgaaagcc atacctgcca tagttgacgc attgtgcatc ttttcacgtg cttcaaaatc
    20041 ggctgtttta actgattttt caagattatc aaaaacaatt ttaatggctt gaagagctaa
    20101 accatccgta aagtcattgg ccatttgtga tacgtaagct tcggttgcgt gtgtcaaaac
    20161 gtccatacca gtatcagcag caataaagcc tggaacagtc aataccaaag ctgggtcaac
    20221 aatggctaca gttggtgtta atgagtaatc agcgattgga tatttacgat tatttgcttt
    20281 gtcagagata acggcaaatg gtgtcacctc tgaacctgtt ccagaagttg ttgagatagc
    20341 aacaaatttt gttttcttac caagttctgg gaatttgaac gcacgtttgc ggatatccat
    20401 gaatttttga acgaggtcat ggaaatcaac ttctggttgc tcgtagaaga gccacataac
    20461 tttggctgca tccataggag atcccccgcc aagggcaata atggtgtctg gtttgaaggt
    20521 acgcattaat tcagtacctt tcataacagt tgtaatgtct ggatctggtt ctacttcagc
    20581 aaagatctga taaacaactt tattgcgacg aagatcaagt tgttcaatga tacgatccaa
    20641 gaaaccaagc tcaaccatag cgtgatctgt aacaatcatg acacgttcaa cgtcacgaca
    20701 tttttgcaaa tattgaattg aatcacgttc gaagtatgtt tttgaaggaa ccttaaacca
    20761 ttgcatatta tttctacgtc ttcctacttt tttgatgttc aagagattta tagcactaac
    20821 gttatcacca actgcgttac gtccatacga accacaacca agagtcaatg atggtaagaa
    20881 agcattgtaa acatccccga taccaccaaa tgtagaaggt gaattccaga tgacgcggat
    20941 agcacggatt ctagttccaa attcttttgc caactctgca tctgctgtat gaatggcagc
    21001 tgagtgacca agtccattaa attcaaccat ttgacgagct ttttcaacac cgtcttcacg
    21061 tgattcagat ttcaaaacag caatgactgg tgataatttt tcccgtgtca aaggttcttt
    21121 ttcactaact tcagtacatt ctgcggcaag aatatttgtt ccttctggaa cagtaaatcc
    21181 agcttgtgct gcaatccaag tagctggttt tcctacaata tttggattta attttgcacc
    21241 agcacagttt ttgctatttg cttttgcacc aaaacagaat tcttcaagaa gggctttttc
    21301 ttttttatta acaaaataag tgtggtaaga tttgaattct gctacaaaat cgtcatagat
    21361 ttctttatcg atgataacag cttgttcaga ggcacagacc ataccattat caaatgattt
    21421 agacatcaca atatcatgag cggcttgacg gatattagct gatttttcaa cataagctgg
    21481 aacgttacca gcacctaccc caagggcagg tttcccacat gagtatgccg ctttaaccat
    21541 cgcatttcca cctgtcgcaa ggatggtggc aatgccgtca tgattcatca aagcatttgt
    21601 tgcttcaagt gatggtgttt caacccactg cacacaattt tcaggagcac cagcggcaat
    21661 agcagcatca cgaacaatac gagcggcatg cgctgatgat tcttgtgctg atgggtgaaa
    21721 ggcaaaaata attggattac gtgtttttaa cgagatcaaa gatttaaaga tagctgttga
    21781 agttgggtta gttgttggtg taatcccaca gataacgccc accggctcag caattaatgt
    21841 caaacctgtc acatcatctt cttcaataat tcctactgtt ttttgatggc gcatgttatt
    21901 aaccacatgc tcacaagcaa atagattttt tgttgcttta tcttcaaaga caccgcgacc
    21961 tgtttcttca taagcatgtt tagcaagttc accgtgagca tccaaagcag caacagaagc
    22021 ttttgctacg atataatcaa cttgttcttg gtcaagctta cgcatctctt caagagcagc
    22081 taaacctttt tgaacaagag catcaattgt cacagaaaca gaggttgttt caacagtatt
    22141 atgtccttca gtcatttgag actcctttat cttgttaaaa gtttcacaat tttg
//
LOCUS       DAUELZ010000016        22153 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.016, whole genome shotgun sequence.
ACCESSION   DAUELZ010000016 DAUELZ010000000
VERSION     DAUELZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22153
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.016"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(355..1480)
                     /locus_tag="VHI09_000510"
                     /pseudo
     CDS             complement(355..1480)
                     /locus_tag="VHI09_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1785..2582
                     /locus_tag="VHI09_000511"
     CDS             1785..2582
                     /locus_tag="VHI09_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ6120412.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYRGEILFADMMTKEQYTEVAKKILANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            2586..3410
                     /locus_tag="VHI09_000512"
     CDS             2586..3410
                     /locus_tag="VHI09_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ6120413.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAVREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLTNDESGVAEAVKTFILEEES"
     gene            3410..4960
                     /gene="ftsY"
                     /locus_tag="VHI09_000513"
     CDS             3410..4960
                     /gene="ftsY"
                     /locus_tag="VHI09_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEQ6120414.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQDK
                     IEAVQQEDVSSEGAGSVENGPEAASVNAPVEEETGDNSNHPSEDTNKFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATYDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KGVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(5014..6381)
                     /locus_tag="VHI09_000514"
     CDS             complement(5014..6381)
                     /locus_tag="VHI09_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ6120415.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVTEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLELFILSFVLFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTSLGALLAPAFGKLYDQKGARLSLYLG
                     NSLFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            6709..7551
                     /locus_tag="VHI09_000515"
     CDS             6709..7551
                     /locus_tag="VHI09_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEQ6120416.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNSFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLLVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            7553..9415
                     /locus_tag="VHI09_000516"
     CDS             7553..9415
                     /locus_tag="VHI09_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ6120417.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLTMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPILAATIAGILLHPAFVAMVAEGKPLALFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFTNALIAGAATIVLAFSLTWFMGIDEE
                     SPEQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GVVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVK
                     RGQALLKMDTDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKMVTKEDALLVIL"
     gene            9434..10858
                     /locus_tag="VHI09_000517"
     CDS             9434..10858
                     /locus_tag="VHI09_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ6120418.1"
                     /translation="MGIFSKDFLWGGAVAANQVEGAFEADEKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMGFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(10957..11772)
                     /locus_tag="VHI09_000518"
     CDS             complement(10957..11772)
                     /locus_tag="VHI09_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEQ6120419.1"
                     /translation="MIRFLILSGYFELTMYLQLSGKLDQYINVRYSYLAYISMVLSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLAEVHQTKEPNNPYVYRVF"
     gene            complement(11772..12674)
                     /locus_tag="VHI09_000519"
     CDS             complement(11772..12674)
                     /locus_tag="VHI09_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEQ6120420.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKAFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            complement(12822..12977)
                     /locus_tag="VHI09_000520"
     CDS             complement(12822..12977)
                     /locus_tag="VHI09_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120421.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDKPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            13211..15343
                     /locus_tag="VHI09_000521"
     CDS             13211..15343
                     /locus_tag="VHI09_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEQ6120422.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            15330..15767
                     /locus_tag="VHI09_000522"
     CDS             15330..15767
                     /locus_tag="VHI09_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEQ6120423.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            15831..16103
                     /locus_tag="VHI09_000523"
     CDS             15831..16103
                     /locus_tag="VHI09_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEQ6120424.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            16468..17400
                     /locus_tag="VHI09_000524"
     CDS             16468..17400
                     /locus_tag="VHI09_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEQ6120425.1"
                     /translation="MTVTVKMLVQKVKLDVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            17397..18176
                     /locus_tag="VHI09_000525"
     CDS             17397..18176
                     /locus_tag="VHI09_000525"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEQ6120426.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESFWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            18198..18605
                     /locus_tag="VHI09_000526"
     CDS             18198..18605
                     /locus_tag="VHI09_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEQ6120427.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            18598..19026
                     /locus_tag="VHI09_000527"
     CDS             18598..19026
                     /locus_tag="VHI09_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ6120428.1"
                     /translation="MNKSFKNLVIGAASGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     FAEVEAKEKQDDVIIDLNEEDIIIDYTEQDEPVSDTLDKH"
     gene            complement(19187..19471)
                     /locus_tag="VHI09_000528"
     CDS             complement(19187..19471)
                     /locus_tag="VHI09_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEQ6120429.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLLAKVIKSLLM"
     gene            19670..20596
                     /locus_tag="VHI09_000529"
     CDS             19670..20596
                     /locus_tag="VHI09_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ6120430.1"
                     /translation="MEKIIITATAESIEQVKALLVAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            20698..21984
                     /locus_tag="VHI09_000530"
     CDS             20698..21984
                     /locus_tag="VHI09_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ6120431.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIVICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESLEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRA"
BASE COUNT         6841 a         3923 c         4845 g         6544 t
ORIGIN      
        1 taataatcac atattgttgt caacgaagcc caccatagcc tataatgaaa gcctagtagg
       61 gatgagctat ataccaacta tctatcaatc cagacgtctc tactaaaaaa tactccgtct
      121 tctatgaaac tagaaacgga gtatttctgt ttgtctatac cactaaatga acagttatat
      181 agaaaggtga ttggatgttt ggaataaaca tcatgaaaaa aatttgagac cttcttatat
      241 cctaaactag aacgaatgtc tagtgaaata aaaaaaaacg acaccattag gatgtatatt
      301 ttggactaat gtcaaataag tagacagaaa accgtgttat tttattgcgt taaactaatt
      361 ttcttctttc tgattagggg ttagtcctag attagccgta tgtgggttgt aattgttata
      421 aaaattctca atgtattcaa agcaatctaa ttgaacctgt ttgatatttt gatacttttt
      481 tcggttgatt tgtctatgct ttaaatactt gaaaaatgct tcagttacgg cattatcata
      541 aggatatcca ggtttagaaa aagaatgcat gatagtgttg tcatcaatta tttttctcaa
      601 ttccctggcc ttaaattgtg acccttggtc ggaatgaaag agaagtgttc cttcaatctt
      661 tcttttatta agagctaatt ctagagtgtc acatgctaac tttgcatcca tatgatgact
      721 cagtttccaa gcaatacatt ttctagaata gagatcaagt attgcgcaga gatagacata
      781 tttcttatat ccaatagaaa tataggtgaa gtcagtagac catacttgat ttggtttgtc
      841 aggattaaat ttctggttga gtaagttttg aggatacgtt ttttgagtcc ttttaagagc
      901 tgtttttggc ttaacggtag ccattttagg aagcccatat tcttcatcag acggtaaatt
      961 cttccttcag agatttttgt gtcgtagtct ctttgaagaa tgactttgat agacttcaca
     1021 ccaagtcttt tgttcgcttt ggtataaatc tcaagtattt gttttttata aatttgatta
     1081 tccaattctc ttttggaggg cttatgtttt agaaatttat agtaagtgga gcgattgaca
     1141 cgtaaaacac gatacaaggt tgttgtggcg tgttcaaagc gtaaccgata gacagcgagg
     1201 agtcttactt taagttttgc atgaatatgg cacttgcttt ttttaggata aggttctcct
     1261 cttcaagctg cgcattacgt ttttgtagct cttgtatttg cttggcagta agtacagaat
     1321 tatcttcgag tttgacttga gaatactgtt tgatccattt tgcaagcgca gaagcggata
     1381 ccccatagtc tttacagagt tcagactgag tttttccagt ttgataaagg ttgacaagag
     1441 attgtttgaa ttcttcatcg tagcgtttaa ctgttgacat aggttgtcct ttcgtttttg
     1501 tgtcttttaa aacagattgt aacacacaaa gttttgtcta cttttatagt atacatccag
     1561 tggtcattta taaaaataaa aaaagtagca gttcacttca aaaacggtca ggttatcgaa
     1621 atataatctg gctgtttttt acgaatggaa tgaaagctgc tttttatgat ataataagaa
     1681 aaattgaaca gaatgcagaa atgatggggc ttatttaaat cgttaaaaag tgcacaccct
     1741 aaatggcgtt tgcacccccc tagagtaaaa aaaggagaca ttgtatgata aaactaatag
     1801 caacagatat ggatggtacc ttcctagctg aagatggtac ttataatcaa gagcaactag
     1861 ctgcgctctt gccaaagtta gccgaaaaag gcattttatt tgcggtttcg agtggccgct
     1921 cgctcttagc cattgatcag ctctttgagc cctttttgga tcagattgct gttattgcag
     1981 aaaatggttc cgtggttcaa taccgtggtg agattctatt tgcagatatg atgaccaaag
     2041 agcagtatac cgaagttgca aaaaagattt tagccaatcc tcactatgta gaaacaggta
     2101 tggttttttc tgggcaaaag gcagcctata ttttaaaagg cgcatcagaa gagtacattc
     2161 aaaaaacaaa acattactat gctaatgtta aggttattaa tggttttgaa gatatggaga
     2221 atgacgccat ttttaaagtt tctaccaact tcacaggcca taccgtctta gaaggaagcg
     2281 attggttgaa tcaagccctc ccttatgcca ctgcagtgac caccggcttt gattccattg
     2341 atattatctt aaaagaggtg aataagggct ttggtatgga gcatctttgt caagccttgg
     2401 gcattaagaa agcagaaacg attgcttttg gggataattt taatgattac cagatgttgg
     2461 agtttgctgg tagggctatt gctacggaaa atgcaagacc tgaaatcaaa gtgatttcag
     2521 accaagtcat tggccactgt aatgacggag cagtactaac gtatttaaaa ggactagtgt
     2581 aactcatgaa tattagaatt ttggcgcttg atttagacgg taccctctac aacactgaaa
     2641 aaattgtcac agatgccaat aaaaaagcct tagcagcggt tagagaaaaa ggggttaagg
     2701 tggttattac cacaggtcgc cctctaaagg ccatcggtaa cttgctagaa gagttggact
     2761 tgcttgacca cgatgactac agtattacct ttaatggggg tttggttcag cgaaatacag
     2821 gggaggtttt ggataagagc tctctcagtt ttgaccaggt ttgccagatt cagcaagctt
     2881 tagaggcggt tggcttacca acagatatta tcagtggtgg ggatgtgtat agcattccaa
     2941 gtaaggacgg taggcattcg cagtaccatc tagctaaccc actgttgacc tttattgagg
     3001 tgacatctgt agcggaacta ccaaaagaca ttacctataa caaaattgtg acagtcaccg
     3061 atccagactt tttggatcag cagattataa agctatcccc aagcttattt gaggattttg
     3121 aagcctttaa atcacgagat attatttttg aaatcatgcc aaaaggcatt gataaagctt
     3181 ttggattaaa cttgctttgc cagcaccttg gccttgacgc tagacatgtc atggctatgg
     3241 gagacgaagc taacgacttt gcgatgttag aatgggcagg acttggagta gctatggcta
     3301 atggtgtttc aggagctaaa gccgttgccg atgccgtgac caccttgacc aatgatgagt
     3361 ctggagttgc agaagctgtg aagacattta ttttagaaga ggagagttaa tgggattatt
     3421 tgatcgttta tttgggaaaa aagaaacccc gaaggtagct gaggaaaagt tagaagaaaa
     3481 tcttttgaca gaaacaactc agaaagagga gctttctgaa aaagccaatg agcaagacaa
     3541 gattgaggcg gttcaacaag aggacgtttc ttcagagggc gcaggttcgg tggaaaatgg
     3601 tcccgaagca gcttcagtaa acgcaccggt tgaggaagaa acaggtgaca atagtaatca
     3661 tccatcagaa gataccaata agtttgctgc cgacaaaaca gaccttaagg taagtgaact
     3721 tagtcaatct acggcatcag aaccaaaaga ccttgttgac cagccagttg tggaacagtt
     3781 tccaacaaaa caagcccaag ctgatgcctc aaatgattcg gcgaatgaag aagcagtaga
     3841 cacttcaaaa gagcaatcca gtagccaaca ggttatggaa gactactatc gtcgtaaggc
     3901 tgctcttgaa aaaagtcttc aagaaaaagc agcggcaacg gttccagtga tgccagaaga
     3961 ggttccacaa gaaaatcagg cctctacctc agcagaagct tctcaaaaca aggcaaccta
     4021 cgataccatt cctgagacag atcaagaaaa atacaagcgt agcttgaaaa aaacgcggac
     4081 tggcttctca gctcgtctga acagtttctt tgccaatttc aggcgagtgg atgaagaatt
     4141 ctttgaagac ttggaagaaa tgctgatctt atcagatgtc ggtgtacatg tggcgacaac
     4201 tttgacagag gagttgcgtt atgaagccaa actggaaaat gcgaaaaaac cagatgcctt
     4261 gaaacgtgtg attgtggaaa aattggtaga tatctatgaa aaagatgggc gttataatga
     4321 agccattaat tatcaagatg gattgacggt tatgcttttt gtaggtgtta acggggttgg
     4381 taaaacgact tcaatcggga agctggctta ccgttacaaa caagaaggta aaaaagtcat
     4441 gttggtggca gcagatacct tccgagcagg ggctgtggct cagctagtcg aatggggacg
     4501 ccgagtagat gtgccagtca ttactggccc tgaaaaggct gatccagcgt ctgttgtttt
     4561 tgacgggatg gaaaaagctg tggcaaaagg tgtagatatt ctcctgattg acacggctgg
     4621 tcgcctgcag aacaaagaaa atctcatggc agagctggaa aaaatgggtc gtatcattaa
     4681 gcgcgtgcta ccagatgccc ctcatgaaac gcttttggcc ttagatgcgt caacaggtca
     4741 aaatgcgctt agccaagcca aggaattttc aaaaataaca cctttaacag gcttaatttt
     4801 aacgaaaatt gatgggactg caaaaggtgg ggtggtccta gccatccgtc aagaattaga
     4861 cattccagtc aagtttatcg gatttggtga aaaagtggat gatattggag aattccactc
     4921 agaagacttt atgaaaggtc ttttagaagg tattttataa gaaaaaaacc agtcttgttc
     4981 aacagaacga ggctggtttt taagtaagtc agcttaggct aatccttttt tccctaaatg
     5041 gtaaaacata gcaaagaaaa agataaaatc aagtagagca aaaatagtaa agagaaggta
     5101 gacagactgg acaccgcttg tgaattctgg ctgactattg gctatcagtg atgccatagc
     5161 agttcctaga gcgccagcaa attgctgcat catctgaaaa atggccgtgg catcggcatt
     5221 tttctcggca ggcaattctc gaatagccgt ggctaagcta ttattaaagc ccatgttacg
     5281 cccaaacgtg aataaaatat ataaaagagt gaatggtaaa agcataaaat gtcttgtttg
     5341 aagtgtcata ataatcaatg ataaactaaa taaggaattt cctaaataaa gcgaaagtct
     5401 tgctcctttt tgatcataaa gtttaccaaa agcaggtgct agtagagctc cgagtgaggt
     5461 accaggtagt aacaccatac cagcttgaga actattagca atcttttcca tgacaataaa
     5521 gtttggcgtt agaaaattta tgcctaaatt aatcagctgg aagacaaaaa agggaatcag
     5581 gccaaaggtt agagagggaa ttttgagaat gcgaatatca agaaagggtt gcttagctgt
     5641 gagattcttg tagaggaaga ggacaaagct gagaataaaa agctctaagt aatacaaatt
     5701 aacacttccg ttttctagac tagtaatagc taataaggca gaagttaagc taatcgatag
     5761 tgcaataaat gccaaccagt caaagggtac tttttcggtt actggagaat cttcgaggta
     5821 ataataagct agaatacctg caatcagtgg tacagggagt atacagataa aaatccattg
     5881 ccaactaaaa tggctaatca taaagccccc ataagtaggc ccaaaagcag gtgctaagct
     5941 aataataaga ccagcaaatc ccataaatag acctacctta tgcattggga cacgctctaa
     6001 aataatatta aacatctgag gcattaccag accagtacca atgccttgaa aaatgcgggc
     6061 tagcaacatg atcgcaaagg actgggtcag aacggccaag atggtgccaa atgtaaaaag
     6121 acctgtggcc ataaaaaaga ggggtctttc ccgcacattt tttttcagtg tagccgaagt
     6181 ggtcatcata atcgccactg ctagtaaata aatggtcgtc atccattgca agctatttaa
     6241 gggcaactga tagactgaca tcagtgtcgg gaaggtgaca ttcatactgg tttcagacaa
     6301 aattcctgaa aaaccaatga gtgctatggc gataatcgcc ataattgttt gttttgaaac
     6361 ggtttgttga tgatgtgaca tgataactcc tttatctaat cgattgttat tgtatcatat
     6421 aatcttaaaa ttgtcagaaa ctcttttttg ctaaatcaat agatacgaat aacaatagtt
     6481 gctctttcag agaatttttt gaaaacgctt gacagaatct taaagagatg ataacattaa
     6541 agcgttctca gatgtgatta cttcaggtaa gcaggctaga gacataacaa tcagaatatt
     6601 aaaggatgtt atcacagttt tgtgagcagg gacctattcg aaaagtgctt tttagcgagc
     6661 ttttggatag gtcatttttt attgtgatac taaaggacta ggacttttat gttgattaaa
     6721 cgcgttttaa accataatgc ggcgatttcg accaaccatc aaggattaga tattttattg
     6781 atgggaaaag ggattgcctt tggaaaaaaa gttggagata gtattgagct taatgctatc
     6841 gaaacatcat ttgttttaaa aaatagtgac aatatgaata gcttcacaga gttatttatt
     6901 acggtgccac aagaagttgt tgcttgctca gagagaatta tcaatctagg taaaattaaa
     6961 ttaggcaaaa ccttagatga aattctctat attaatttaa cggatcatat tcactctgct
     7021 attgaaaggc atgagcaagg aatgttgatt cataacccac tacggtggga aattcaacgc
     7081 tattatcctg atgaatatag tctaggggta aaagccttag agctaattga gcgtaatcta
     7141 ggagtcactc tagctattga tgaagcagct tttattgcca tgcattttgt caatgctagt
     7201 ttggacactc cttttaagga gccacatcgc ctgacggaga ttgtttctta tattgagcaa
     7261 aaaatcaaaa cagacttcaa aacagaattg gatgacacat ccatcgatta ttaccgcttt
     7321 atgacccata ttaaattatt tgctcaacgt gtgttatccg agatgtctta cgatgatgat
     7381 gatgcggaac tgcttttagt ggtgaaaacg aaatacccta aagaataccg gtgcgtctta
     7441 gatattagtg aggagataaa aaagcggtac aactaccacc ttaattcaag cgaacttttg
     7501 tatttgacgg ttcatgttaa acgtttagtg aaacatttaa aggagactta atatgacgta
     7561 tcaagaaact gccaaggcta ttttagcggc ggttggtggc aaaacaaata ttcaacgtgt
     7621 gacacactgt gtaacacgtc tacgcttagt actgaaaaat gatgagaaag ttaaagatca
     7681 gcaagtcaaa gccatttcaa atgtgattgg tgtgatgcgc aaaaacggcc agtaccaaat
     7741 tatcttgggc aatgatgtca ataattatta ccaagccttt ttaagcctag ggcattttga
     7801 caatcaagat gaagatcatt cttcaaaagc gaaaggaagt atccttgagc gcttgattga
     7861 aaccattgct ggcgtgatta caccgctgat tccagccctt cttggtggtg gaatgctcaa
     7921 agtggtggga atcttgctta ctatgcttgg cttagcgagt gcggactctc aaaccgttgc
     7981 ctttattaac ttctttggcg atgcggctta ttattttatg cctgtcatga ttgcctattc
     8041 agcagcggcg cgctttaagg tgacacctat tttagcagcg acgatcgcag ggattttatt
     8101 gcatcctgcc tttgttgcaa tggtagcaga aggtaaacca ttggctttat ttggagcacc
     8161 agtcactcct gctagctatg gctcatctgt tattcccatt ttgatgatgg tttacttgat
     8221 gcaatacatt gaaaaatggg tcaatcgctt ggtgccaagc gtgatgaaaa gcttcttaca
     8281 gccaacctta atcattttga tttctggctt tttggcttta gtggttgtag gtcctcttgg
     8341 tgtgattatt ggtcaagggt tatctaatac catgctcgct atttatcatg tggctccgtg
     8401 gctagcactt gccattttgg gagcgattat gccgcttgtt gtcatgacgg ggatgcactg
     8461 ggcttttgca ccaatctttt tggccgcttc ggtcgcaaca ccagatgtct tgattttacc
     8521 agcaatgttg gcttctaatt tggctcaagg agccgcatct ttggctgttg cctttaaaac
     8581 aaagcagaaa caaacacgtc aagttgccct tgccgcgggg atttcagctt tgctggcagg
     8641 tatcacggaa ccggcacttt atggggtcac actgaaattt aaaaagccac tctatgcagc
     8701 catgatttca ggtggtctgg tcggagcctt tattggtttt gtcaatattg cttcttatac
     8761 ctttgtcgta ccttctatta ttggtttacc acagtacatc aacccatcag gcggagctaa
     8821 ttttacaaat gctttgattg ccggagctgc gacgattgtg ttagccttca gtttgacttg
     8881 gttcatggga attgatgaag aatccccaga gcaagtgagt gttgcagcag atatgtcaca
     8941 agttaagagc ggcttgtcaa ccaaacaaac gttgtatgct ccaatgacag gtgagatgct
     9001 ttttctatca gaagttcctg atgaaacctt ttcttccaag ttattaggag aaggatttgc
     9061 catattacca agcgaaggtg tggtctatgc cccctttgat ggtgaagtca tcactttctt
     9121 tccaaccaag catgctgttg ccttaaaaaa cacacgtggt gtggaagtct tgattcatgt
     9181 cggtattgat acggttgagt taaaagggca aggtttcgag cagttagtgt ctgttggcga
     9241 tgtcgtgaaa cgaggccagg cacttctaaa aatggatact gatttcatta cttcaaaagg
     9301 ctattcactc atcagtcctg tggtcgtgac caattcggct gagcagttgg agattattat
     9361 tcaagatgac aaaaaaatgg tgactaaaga ggacgctttg ttagtcattt tataacctaa
     9421 aaggagaggg agaatgggaa ttttttcaaa agatttctta tggggcggcg cagtagctgc
     9481 caaccaagtg gaaggagcat ttgaggcaga cgaaaaaggt ttgtccgtac aagatgttct
     9541 accaaatggt ggactaggag aatggacaga tagtcctacc agcgataatt taactttaga
     9601 ggcgattgat ttttaccacc gctacaaaga agacattgct ctttttgccg agatgggctt
     9661 taaagtcttt cgaacgtcta ttgcttggtc tcgtattttt ccaaatggtg acgatgacca
     9721 gcccaatgaa gcaggtttgc aattttatga tgacttattc gatgaattgt tgaactatgg
     9781 tatcgaaccc ttagtgacct tgtcgcatta tgagacacct ctgcatttgg ccaaagccta
     9841 taatggttgg acagatagac gtctgattgg cttttttgag cgctttgctc agacagtcat
     9901 ggaacgctat aaggacaaag tcaaatactg gttgaccttc aacgaagtca actcgatttt
     9961 acacatgccc tttaccagtg gtggcatcat gacagaaaaa gaaaagctga gcctgcaaga
    10021 tttataccaa gctattcatc atgaattggt agcatctgct agtgtcacaa agctagcgca
    10081 tgaaatcaat cctgatgtta aagttgggtg tatgattttg gcgatgcctg cttacccaat
    10141 gacctctgat cctcgtgata tacttgctgc gcatgcattt gaaaatctta atctcttatt
    10201 ttcagatatt catgtgagag gcaagtatcc ttcatatatt aagtcgtatt ttaaagaaaa
    10261 tgggattgag attgtttttg aagacggaga caaggaatta ttagcggaac atacggttga
    10321 ttttctatcc tttagctatt acatgagtgt cacacaagcc cacaatcctg aagcttacac
    10381 atctggtcag ggaaatattc taggtggttt gtccaatcca tatttagaat cttcggagtg
    10441 gggttggcag atagacccaa ttggtctgcg attggtcttg aatcagtact atgaccgcta
    10501 ccagattcct ttgtttattg ttgaaaatgg cttaggtgca aaggatcagc tggtgcaaac
    10561 cgcagatggc agcatgacgg ttcacgatga ttatcgcatt gactacatga gtcagcatct
    10621 cgtacaagtt gccaaagcga ttgaagatgg cgttgaggtc atgggttaca ctgcttgggg
    10681 ctgtattgat tgtgtctcta tgtcaacagc ccaattaagt aagcgctacg gttttattta
    10741 tgttgaccga aatgatgatg gtactggtca actcacacgc tataagaaaa aatcatttga
    10801 ttggtaccga caagtcattc aaaccaatgg acgttacctt gaggataatt tttcctaaat
    10861 aggtcttgtt taagatggtg actctgttcc tatgctaact gataaaactc aaaaagcatt
    10921 tgttttgata aaaaacaagt gcttttttat tagtctttaa aaaacgcggt aaacataagg
    10981 attattaggt tctttggttt gatgaacctc agcaagttga aggacaggta gatgttgttc
    11041 taagttttta tcatattcca tatgaaggcg acctttaact gttagccatg tgttgttagg
    11101 gtagctggtt tgattacctg tggtcaaaag tccgtacact cctgagtcgg caatacagtg
    11161 aataatgccg aagcggaaaa ggaattggta gttgtcatgg cccggttcgt tataaacaaa
    11221 gccagtgtat tggatgtcac gatcaagaaa ctcatctggg tagagataga tcaattccat
    11281 cacttccata taattttcag tggtaatggt gaccggtttc ttgcctttat atttatggag
    11341 ttcttgacgc atctcctttt ggtaggcaga ctttgtaaag taaaggctgg tatcaggttt
    11401 gaggtattga atggtggtac cgtcatcact gactcccgtt ttagaggctc ccgcagccaa
    11461 tgggaaagta tagcctttag cagaaaccgt ggtggaatct aaggtcacgg tcggcacaag
    11521 gagaccaatc aaaacaggaa agaccaagat aaaaggactg gtcagccttg cgatttttcc
    11581 tgttaaatgg ctatgtacct taatgttttt catccaggta tagagttgaa caagagctaa
    11641 aatgaaagat aaaaccatgg aaatataagc aaggtaagaa taccgaacat tgatgtactg
    11701 atccagtttg ccagacagct ggagatacat ggttagttca aaatagccag ataaaattaa
    11761 aaagcgaatc attagagcac ccccacaagt aaacaataaa cggcaatcat aagaacggat
    11821 acgccgataa attggacaat gaagcgtcct ttaaaagcct ttaccatcat cataaggttt
    11881 ttaatatcca ccataggacc aattaaaaga aaggcaagaa ctggagccac tccaaaggtg
    11941 gataataagg aagctccgat aaaggcatcg gcttcactac ataatgatag aatgaaagcc
    12001 atcagcatca tgatcaaaat ggctgtcaag ggattgtgcc caatcgttgt caacacacga
    12061 gttgggacat atatttgcat tgcagaggca atcaaggttc caaatacgag atacctgccg
    12121 gtgtcgaaga attcatcaat agcatgtacc agtgctagat aaatgcgttt tggcaagctt
    12181 tcatgagaat aatcatgaaa atgcacgggt tgagcattat ccttgagaat gttatcatct
    12241 acaataaaag ctaacattac cccaagtgta atggctacta gtgcagcacc gaccaagcga
    12301 agtatcagaa agcgcaaaga attcccaaaa gcagaataag tagcgaacaa aacaatagga
    12361 ttaataatag gcgctgttgc cagaaaaggg acggccgtat aactaggcac ctttttttcc
    12421 aaaaagcggt tgatgatggg aataatcccg cattcacaag aaggaaaaac aaatccaaca
    12481 aaggttccaa acagaatgcg caagcatttt tgcttaggta ggtatttttg aaccaattct
    12541 ggcgttacaa agacttcaat acaaccagat aaaatagttc ctaggagcac aaaaggaaga
    12601 gcttcaatga taatggacat aaaaatcgca aaccattgca agacactggg aggaaggttg
    12661 gaaaagagtg acattcacca tatcctttct agatgataaa agaggaggct gaaacacgaa
    12721 tgttcagcct cgactgatac gttttgttag cctgatcgct aaaaatctct caactcagtt
    12781 taaacgaatg gacgatatgt taagagaatg agacaaacta gctatggttt gcttggtttt
    12841 gtttgttctg tcgttttttc tttggtcgct agtttaccat cagtattgac ttccaaaggc
    12901 ttatcaaaac tagggattga tgcaaagcct gctagcattt tttcaagttc atcatttttt
    12961 ttatgggtaa ttgccatatc tatgaccttc tttcattcaa gataagtcta ttatactatc
    13021 atttgagaac aatgccaaag cggaagtgtt gtttacctct ttctttttta aaattaggta
    13081 ttgttattta gggtttcgtc agttatttac taaaagtagc tttttttgat tctttacgca
    13141 actttatatt aggacaatgt ttcgctatca ttttttgcat taacatggtt tgtgttataa
    13201 tagcaatatt atggaaaata acaataatca caacattgca gaggctttgt cagttagtct
    13261 ccatcaaatt gaacaggtgc ttgccctaac agcacaaggc aataccattc cctttattgc
    13321 acgttaccgt aaagaagtaa caggaaatct agatgaagtg gtgattaagt ccattattga
    13381 tatggataag tctctcacta ccctgaatga gcgcaaagca accattctcg ctaaaattga
    13441 ggaacaggga aaactgacag atcagttacg aacgagtatt gaagcaaccg agaaactcgc
    13501 tgatttggaa gagttgtacc tgccttataa ggaaaaacgc cgtacaaaag cgacgattgc
    13561 gcgtgaagca ggcttgttcc cattagcacg tttgatttta caaaatgcgc aaaaccttga
    13621 aacagcagca gaaccctttg tcacagaagg atttgccagc ccacaagaag ctctagcagg
    13681 agctgtggac atccttgtgg aggccatgtc agaagatgct aaattacgct cttggaccta
    13741 caatgagatt tggcagtaca gccgcttagt atcaacgctt aaagatgagc agttggatga
    13801 gaaaaaggtt ttccaaatct attacgattt ttctgaccaa gtgtctaaca tgcaaggata
    13861 tcgtacttta gcccttaacc gtggcgaaaa gttaggcatc ttaaaagtgt cttttgagca
    13921 taacttggag aaaatgcaac gctttttcag tgtgcggttc aaagaaacca acccttatat
    13981 tgaagaggtc atcaatcaga ccatcaaaaa gaaaattgtt ccagctatgg aaagacgggt
    14041 tcgttcagaa ctcagtgatg ccgcagaaga tggggctatc cacctctttt ctgaaaatct
    14101 ccgtcatctt ttgttagtgt ctcctttaaa agggaaaatg gttcttggat ttgaccctgc
    14161 ctttcgaaca ggtgcaaaat tggctatcgt tgatcagact ggaaaattgt tgactactca
    14221 ggttatttat cccgtagcac cagctagcca aacgaagatt caagcagcta aagaaacact
    14281 gactcagctc attgagactt accagattga tattattgcc attggaaacg gaacagccag
    14341 tcgggaaagt gaagcctttg tagcagacgt tttgaaagat ttcccaaata cgtcctatgt
    14401 catcgttaac gaaagcggag cttctgttta ttcagcgtca gagttagccc gtcatgagtt
    14461 tccagacctc acagtggaga agcgctctgc tatttctatt gctcgtcgct tgcaagaccc
    14521 actcgcagaa ttggttaaaa ttgatcctaa atctattggc gtcggtcagt accagcacga
    14581 tgtgagtcag aaaaagttga gtgagaatct tggttttgtt gttgatactg tggttaacca
    14641 agtcggtgtt aatgtgaaca cggctagtcc atctttatta gcacatgtgt caggtttgaa
    14701 caaaacgatt tcagaaaata ttgtcaaata ccgcgaagaa aatggagcac tgacatcacg
    14761 cgctgacatc aaaaaagtgc cgcgtttagg agccaaggcg tttgagcaag cagcaggttt
    14821 tctcagaatt ccaggagcca aaaatatttt agacaacaca ggagttcacc ccgagtccta
    14881 cccagcagtc aaagaactct ttaaggtact tggtattcag gacttggacg acgctgccaa
    14941 ggcaacttta gcagcagttc aagttcccca aatggcagaa acattggcta ttgggcagga
    15001 aacccttaaa gatatcattg ctgatctcct taaaccgggt cgtgaccttc gtgatgactt
    15061 tgaagcacca atcttacgtc aagatatcct tgatttgaaa gatttggaaa ttggccagaa
    15121 gcttgaagga actgtgagaa acgtcgttga ctttggtgct tttgtagaca ttggtgttca
    15181 tgaagatggg cttattcaca tttctgaaat gagcaaaacc tttgtcaacc accctagtca
    15241 agttgtttca gtgggtgact tggtaactgt ttgggtctct aagattgact tggaccgcca
    15301 caaggtcaat ctcagcctat tgccaccgcg tgacactcac taattatgtc caagaagttt
    15361 ccctggctga ttttggcaaa ccatttcacc acaaggctta ttggaacaag cgcctaaaaa
    15421 caactggcgg tcgctttttt ccaaaagatg gtcatttgga ttttaaccct aggatgcttg
    15481 aggagcacgg tgagcttatt tttcggaaaa ttgttcgcca tgagctctgc cattaccatc
    15541 tttattttga aggaagaggg taccatcata aagataggga tttcaaagat ctgctagctc
    15601 aagtaaacgg gcttcgttat gtcccaacta gctctaaatc aaagactaat catcattata
    15661 gttgccaaac ctgtgggcag gtttatcaac gaaaaaggcg tatcaatctg gctaaatatg
    15721 tttgtggaaa ttgccacggg aaacttatgg aaaaaaatca gtcgtaagac tggttttatt
    15781 ttgcgaaaaa caatgtaaac tagtatcata gaatgaggag gtaaagaaac gtggaaacta
    15841 aattctataa acagcgaaaa aatcgcttgg ttgcaggtgt cattgctggt ttagcagata
    15901 agtatggttg ggatcttgct ctagcgcgtg tcttagcggc tcttcttatt tacggaactg
    15961 gttttggagt tttgctttat attttgttgg ccatcttctt accttacaag gaagacttgc
    16021 ttgaagaacg ttatggccga ggtcctcgtc gtcgtaaaga cgctgatgtc ttaaatgaag
    16081 aagaagatgg ctggttctgg taaacaatca gttaaaatga taattcatca cgtaagagca
    16141 accatgtgat taactgatga taataaaatg ttttgatatg taagtaagcg aggggtctta
    16201 tcagctatga tgaggctttt tgctttttga cttagtaaga tgataccttt ttaaatacca
    16261 taataagcac taattgctca atgatactag taaagtattt gtttgccatt gtcttatttt
    16321 tgactcatat ctctaaatga taaaacctag tttttaatag agctaagacc atttttggtg
    16381 tcgttgctct catttagtag tgaaaaaatg agtatttgca agggtctaac ggtcgatttg
    16441 tgttataata ttgaaaaagg agaaattatg accgttactg taaagatgct agtgcaaaaa
    16501 gttaaacttg atgtggttta tgctacagat aacttgttgt caaaagagat aacaacatca
    16561 gatatttcac gtccaggtct tgaaatgact ggttattttg attattatgc tccagaaagg
    16621 ttgcagctat ttggaatgaa agagtggtct tatcttacac agatgacttc tcacaaccgt
    16681 tattctgttt taaaagagat gttcaaaaag gacactcctg cagtggttgt ctctagaaat
    16741 ttagccattc caaaagaaat ggttcaagct gcaaaagaag aaggtatttc acttttaagt
    16801 agccgtgttt caactagccg cttagctgga gaaatgtctt attttcttga cgcttcttta
    16861 gctgaacgta ctagtgttca cggcgtttta atggatattt atggtatggg ggttcttatc
    16921 caaggggatt ctggaattgg taaaagtgag actggtttag aattagttaa gcgagggcat
    16981 cgtttggttg cagatgaccg tgtggatgtc tatgccaaag atgaagaaac cctttgggga
    17041 gagccagcag agatcttgcg ccacttgctt gaaattcgtg gggtaggtat tattgatgtg
    17101 atgtcgcttt atggagctag tgctgtgaag gactcatcac aagttcaatt ggccatttac
    17161 cttgaaaact ttgaggctgg taaagttttt gatcgtctcg gaaacggtaa tgaggagata
    17221 actttctcag gagtgagaat ccctcgtatt cgtattccgg taaaaacagg tcgaaatgtt
    17281 tcagtggtta ttgaagccgc tgcgatgaat catcgggcta aagaaatggg ctttgatgct
    17341 acaaaaactt ttgaagatcg tttaactcaa ctaatcacca aaaatgaggt gagtcaatga
    17401 tcaatcccat tgccttaaaa tgtggtccac tagccatcca ctggtatgct ctatgtatct
    17461 tgtcaggact tgttttggcg gtttacttag catcaaaaga agcacccaaa aaaggaatat
    17521 catctgacgc tattttcgat tttattctga ttgcttttcc acttgctatt gttggtgcaa
    17581 gaatttacta tgttattttt gaatggtctt attatgtcaa gcatttagat gaaatcatag
    17641 ccatttggaa tggcggtatt gctatttatg gcggtctcat tacaggagct cttgtacttt
    17701 tggcttactg ttataacaag gtgctcaatc ccattcattt tttagatatt gccgcaccaa
    17761 gtgtcatggt cgctcaagct atcggtcgct ggggaaattt tatcaaccaa gaagcctatg
    17821 gtaaagctgt gagccagtta aattacttac caagctttat tcaaaagcaa atgtttatag
    17881 agggaagtta ccgcattccg acctttctct atgaatcatt ttggaatctt ttgggatttg
    17941 tcattattat gatgtggcgt cgtaagccaa aaagcctatt agatggagaa atctttgcat
    18001 tttacttaat ttggtatggt agtggaaggc tagtcattga aggtatgcga acagatagtc
    18061 ttatgttttt aggtattcgc atctcccaat atgtgtctgc cttattaatt attattggct
    18121 taatctttgt tataaaaaga cgtcgtcaaa aaggaatttc ttattatcaa gaataaggat
    18181 agaaaaagga gaaacttatg gacttagttg gaatctcact gatgattatt gcactcgcat
    18241 ttgttgcttt ggtgattttc ttgattattg tattaaagaa agtctcagaa acaattgatg
    18301 aagccaaaaa aacgatttct gtattgacaa gtgatgttaa tgtgacactt catcaaacta
    18361 atgatatttt agcaaaagct aatatccttg ttgaagatgt taatggtaaa gtagcaacca
    18421 tcgatccatt gtttgttgct attgctgatt tgtcagaaag tctttcagat ttaaatagtc
    18481 aagcaaggca ttttgggcaa aaagcaacta atgctacagg taatgtttca aaagctggaa
    18541 aattagcatt agttggtaaa gtagcctcaa aagtatttgg aaaaaaagga gaaaagcatg
    18601 aataaaagtt tcaaaaattt agtgattgga gctgcttcag gagtagcggc agcttacttc
    18661 ttatcaacag aaaaaggcaa agctttgaaa aatcgtgctg aaaaagctta tcaagcctac
    18721 aaagaaagtc cagatgatta ccaccaattt gctaaagaaa aaggtagtga atacagccat
    18781 ttagcacgtg acactttcta tgatgtgaaa gataagttgg cttctggtga cttaactaaa
    18841 gaggacatgt tagacttgct caaagataaa acaacagctt tcgtgcaaaa aacaaaagaa
    18901 acctttgccg aagttgaagc gaaagaaaag caagatgatg ttatcattga tttaaatgaa
    18961 gaagacatca tcattgacta tactgaacaa gatgagcctg ttagtgatac attagacaaa
    19021 cactaactaa aatgacttaa aacatagaag aagaaaagat tgaaggatct gatcactagt
    19081 cccgatggtt agttttaggc taactgaaga agggggggat tgtggcttca atcttttttt
    19141 gtttttgaca caaaaaaaaa gagtcacgta aaacggactc tggaagttac atcagtaatg
    19201 atttgataac cttggctaat aaggaagtaa tgcctaagct gatgatgctt gcaaataaga
    19261 aagcaaacat tggcgctaaa ttcattacga gcaggacaaa tgcaaataaa acggtaataa
    19321 ggctgaaact agcgatacga gcaaagaaga ctaattcttt tagctttgca ctttggccat
    19381 tgaactcttg aaaatctcga aatttacttt gagattcttg gtaagaatca tcctgttcaa
    19441 ccgaattatg cttctttaac ggtgcaggca tagtcccctc ctttccttaa catactaagt
    19501 aattctatca taaaagtctc ttcttgtcat ctttttttaa aagatttgaa aagatttact
    19561 aatgacaagt gacttatcat gctataacag taaggggact agcttaacgt ttgggctttt
    19621 gcaagaaata tgcttaaggt cttaaaattt ttggtataat aaagcttata tggaaaaaat
    19681 aattattaca gccacagctg aaagcattga gcaagtcaaa gctttattag ttgctggtgt
    19741 tgatcgtatt tatgttggtg aagccaatta cgggttacgc cttccccata atttttctta
    19801 tgatgaattg agacaaattg ccaaactagt gcatgatgca ggcaaagagt tgacagtagc
    19861 ttgcaatgct ttgatgcatc aagatatgat ggatcagatc aaaccctttt tagacttaat
    19921 gatagagatt gctgttgatt atcttgtggt tggtgatgca ggtgtgtttt atgtcaataa
    19981 aagggatggc tataacttta aactgattta tgatacctct gtttttgtga cttctagccg
    20041 tcaggtaaat ttctgggggc aacatggtgc tgttgaaagt gtcttagcaa gggaaatccc
    20101 atcagcagag ttattcacct tagctgaaaa tttggaattt ccagcagagg tgctagttta
    20161 cggcgcctcg gtgattcatc actcgaaacg tcccttgcta gaaaattatt accattttac
    20221 aaagattgat gatgaggtta gtcgtgaacg aggacttttc ttggcagaac caggagatgc
    20281 tagcagccat tactccattt atgaagacaa tcatggcact catattttca ttaataacga
    20341 tatcgacatg atgtctaaat tgggtgagct gtatgcgcat ggcttaactc attggaagct
    20401 agatggcatt tactgtccag gtgatgattt tgtcgctatt actaaattgt ttattcaggc
    20461 taaaacctta ttagaagctg ggcaatttac ccaagaagaa gctgaaaagc ttgatcaagc
    20521 agtgcatgcc catcatccag ctggccgtgg attagatact ggcttttatg agttcgatcc
    20581 aaaaaccgtt aaataagtaa tccttgtctt gagtgagttt actatttcag ggcaagacag
    20641 ggttatgaca ccattacaca ggtaatctac ctatcttgaa aatagaaaga atatcacatg
    20701 tcacatatga aaaaacgtcc cgaggtctta tcacctgctg gaacacttga aaaattaaaa
    20761 gttgcgattg actatggcgc agatgctgtt tttgttggag gacaggccta tggcctaaga
    20821 agccgcgctg gtaacttctc tatggaagaa ttgcaagaag gcattgatta tgcacatgcg
    20881 cgtggagcta aggtttatgt tgctgccaac atggttaccc acgaagggaa cgaaattggt
    20941 gcgggcgagt ggtttcgtca actgcgtgat atggggcttg atgcggtcat tgtttcagat
    21001 ccagccttga ttgttatttg ttcaacagaa gccccaggtt tggaaattca tttgtcaacg
    21061 caagcttcat ctaccaatta cgagaccttt gaattttgga aagccatggg tttgacgcgt
    21121 gttgttttag ctcgcgaggt taatatggcc gagttagcag aaatccgtaa gcggacagat
    21181 gtggaaattg aagcctttgt ccatggagcc atgtgtattt cttattcagg ccgctgtgtt
    21241 ttgtcaaacc acatgagtca ccgtgatgcc aacaggggcg gctgctcaca gtcttgccgc
    21301 tggaagtatg atttgtatga catgccattt ggaggagagc gccgctcctt aaaaggggaa
    21361 attccagaag actattctat gtcctctgtt gacatgtgta tgattgacca tattcctgac
    21421 ctgattgaaa atggagttga tagcttaaaa attgaaggcc gaatgaaatc tatccactac
    21481 gtctcaaccg taaccaactg ttacaaggcg gctgtagatg cttacatgga aagcctagaa
    21541 gctttttatg ctatcaaaga ggaattgatt gacgagttgt ggaaggttgc ccagcgcgag
    21601 ttagctacag gtttttacta tggtatccca actgaaaatg aacaattatt tggtgctcgc
    21661 cgcaaaattc cacaatataa atttgtcgga gaagtagttg cctttgactc agctagcatg
    21721 acagcgacca ttcgtcagcg taatgtcatc atggaaggcg atcggattga atgttatgga
    21781 ccaggtttcc gtcattttga aacggttgtt aaggacttac atgatgcgga tggccaaaag
    21841 attgatcgtg ccccaaatcc aatggaactc ttaaccattt ctttgccaag agaggttaaa
    21901 ccaggagata tgattagggc ttgcaaggaa ggtctggtta acctctatca aaaagacggt
    21961 acaagtaaaa ctgtcagagc ttaattgaaa taaatatgaa aggccctttt tccctagtgg
    22021 cgtaaaagct gcgtttggga taaaagagcc agttttttga aaattagata ccacgatagc
    22081 ttattttctc aaaacttgat aaaataaggt catcataact aaaaagttga aaaaaagatt
    22141 gcttttgtca att
//
LOCUS       DAUELZ010000017        21296 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.017, whole genome shotgun sequence.
ACCESSION   DAUELZ010000017 DAUELZ010000000
VERSION     DAUELZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21296
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.017"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(89..724)
                     /locus_tag="VHI09_000531"
     CDS             complement(89..724)
                     /locus_tag="VHI09_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEQ6120432.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQLIVGPNFNRAVA
                     LICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLPQVEVMV
                     TGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            complement(729..1730)
                     /locus_tag="VHI09_000532"
     CDS             complement(729..1730)
                     /locus_tag="VHI09_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEQ6120433.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEVNIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMTLTAWQGNRVGLYYLENGFGCRSSQV
                     YYDRCGSSFSALDKDSLDLAAIFEGISHFHFSGISLALGKKTQDLIEILVREAKKRDI
                     CVSFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADTTMI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNHFKAYTYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGDEKQYNLDFAVATASLKCTVAEDQLYHSAQQVEAVLANQRDVQR"
     gene            complement(1759..2400)
                     /locus_tag="VHI09_000533"
     CDS             complement(1759..2400)
                     /locus_tag="VHI09_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEQ6120434.1"
                     /translation="MKIALINENSQAAKNGIIYDALTSVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVTYLKSVLEQ"
     gene            complement(2425..3219)
                     /locus_tag="VHI09_000534"
     CDS             complement(2425..3219)
                     /locus_tag="VHI09_000534"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEQ6120435.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGLAAYRELGIEAHGYVCDVTDEVGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            3553..3990
                     /locus_tag="VHI09_000535"
     CDS             3553..3990
                     /locus_tag="VHI09_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ6120436.1"
                     /translation="MIAIIVMGHGHFASGIVSALELIAGKQEKVTAIDFTAEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLIICSKEGIVDWKTLSQQEDGATDDELGGI"
     gene            3990..5189
                     /locus_tag="VHI09_000536"
     CDS             3990..5189
                     /locus_tag="VHI09_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEQ6120437.1"
                     /translation="MTRLLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYSGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPEAREAALKAADKLVQRYQDKGGFIQ
                     AWGELGKKQDYRLIIDCLLNIQLLFFASQETGDNRYPDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPVKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFLNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDPDKKTYE
                     AAMHSMLRALIKDYANKDLNPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            5225..5713
                     /locus_tag="VHI09_000537"
     CDS             5225..5713
                     /locus_tag="VHI09_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ6120438.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKYLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQSIFIVVKDLQDAKRLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHQVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            5732..6514
                     /locus_tag="VHI09_000538"
     CDS             5732..6514
                     /locus_tag="VHI09_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ6120439.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGMILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVAIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWAFAFIGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            6501..7322
                     /locus_tag="VHI09_000539"
     CDS             6501..7322
                     /locus_tag="VHI09_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ6120440.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAVVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVLLTVLIGIIGSWLGILA"
     gene            7409..9316
                     /locus_tag="VHI09_000540"
     CDS             7409..9316
                     /locus_tag="VHI09_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEQ6120441.1"
                     /translation="MSLAFTRFKETVNPDFCRNYLLDYQTDSYADQKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQEAVTDDPEWNFMLNRQTYLQKLILVYLVERDERYL
                     LKAKSFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLFNALTKQEESLIL
                     ASLEKQLQFLHANYLDKYSLSNWGILQTTAILLADAYYGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAAFPTLDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSLCLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLKEQLPEQIDSSWAYLSYPKSNYCHLRQNGHVYF
                     IEGSYQTQFSDRNNYQHDRQILILPPGMFLIIDTIQAQGNHCLVSQFILDNHLDVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKFIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALSWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            9380..10375
                     /locus_tag="VHI09_000541"
     CDS             9380..10375
                     /locus_tag="VHI09_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ6120442.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(10459..13140)
                     /locus_tag="VHI09_000542"
     CDS             complement(10459..13140)
                     /locus_tag="VHI09_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEQ6120443.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTH
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(13371..13757)
                     /locus_tag="VHI09_000543"
     CDS             complement(13371..13757)
                     /locus_tag="VHI09_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEQ6120444.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGDYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            13848..15149
                     /locus_tag="VHI09_000544"
     CDS             13848..15149
                     /locus_tag="VHI09_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ6120445.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEALFHTDHEAFTQEIITNPETEINAILVRHAPDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMTSEDHILSDLASRFINRKILKSVTFDQDSRGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMMQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            15604..16413
                     /gene="yidA"
                     /locus_tag="VHI09_000545"
     CDS             15604..16413
                     /gene="yidA"
                     /locus_tag="VHI09_000545"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEQ6120446.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHVVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFMSRK
                     LGVHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            16413..17639
                     /locus_tag="VHI09_000546"
     CDS             16413..17639
                     /locus_tag="VHI09_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEQ6120447.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVALSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLDIYAGQSYITMASLDLPEQKKTLIQQLDKALAEQVRLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            17626..18861
                     /locus_tag="VHI09_000547"
     CDS             17626..18861
                     /locus_tag="VHI09_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ6120448.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSHLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFKCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(18960..19718)
                     /locus_tag="VHI09_000548"
     CDS             complement(18960..19718)
                     /locus_tag="VHI09_000548"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEQ6120449.1"
                     /translation="MLRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(19959..21155)
                     /gene="tuf"
                     /locus_tag="VHI09_000549"
     CDS             complement(19959..21155)
                     /gene="tuf"
                     /locus_tag="VHI09_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEQ6120450.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
BASE COUNT         6523 a         4618 c         3925 g         6230 t
ORIGIN      
        1 aaaaaacata agcagaagca tattaattga atgaatgata aaaaacgttt ttactagcac
       61 taaagccaag gtaaaaacgt tttttagatt attgtcgcaa acttttgata taatgagcgg
      121 ctttcttagt gattaggtta tattgttttt ggctcgctaa ctgattaaac tcgcctccta
      181 tgcctagaac atctactcca gcggctagcc agtctttgag attatctgaa gaaaccccgc
      241 ctgtaaccat aacctctact tgtggcaaag gagatttgat tgcccgaata aaggacatcc
      301 ctacagtaga accaggaaaa attttgacca tatcaacccc tgattctaat gctgtaacca
      361 cttcattaac cgtcatacaa cctggtaaat aaggtatgct ataacgatga caaatcaaag
      421 ccactgctcg attgaaatta ggaccaacaa taagctgtgc tcctgctaaa atagcttggc
      481 gggcagtgac atcatccaaa actgtcccag cacctatcaa tacctcagga tcttctttga
      541 agcgttcagc taattgaccg ataacctcac tagcaaaggg gttagtatag gtgacttcaa
      601 ttgtcttaat gccaccttca atactggcca ggctgcaagc taaagcttcc tcactagact
      661 ctcctcgtac aaccaggaca agccgattag ccttaagttt ggttaagatt tctatttttc
      721 ccatatcatc acctctggac atctcgttga ttagctaaga ctgcttctac ttgttgggcg
      781 ctgtgataga gctgatcttc agctactgta cattttaggc tagctgttgc cacagcaaaa
      841 tcaaggttgt actgcttttc gtcaccttct aacaactgat aaagtaaacc cgccacaaaa
      901 gcatctccac ttccaacccg ttgcaagact ggggtcgtca cttcacagga ttcgtaaaac
      961 tgcccatcat aggtataagc cttaaaatga ttactgcctt gggcatcatt tgaacgctcc
     1021 gtatggtaaa tagcttgtaa ctgatagcgc tgataaagtc ctgccagacg ttctctaatc
     1081 atcgtagtat ctgccttatt cctatcaaac atgtcaaaat cgtcactgtc taacagcaaa
     1141 ggttccatgc caaaaataat atccgcgtat tgagcaaaat gagaaaagag tcgtttagca
     1201 tcagctaccg caatcatact agatctaaag ttaagatcaa aagaaacaca aatatccctt
     1261 ttcttagctt ctctcacaag tatctcaatc agatcctgag tttttttgcc cagcgccaaa
     1321 ctgatcccac taaaatgaaa gtgactaatg ccttcaaaaa tggcagctag atcaagacta
     1381 tccttatcta aggccgaaaa gctagagcca cagcggtcat aataaacctg actagaacga
     1441 caaccaaagc cattttcaag gtaatacagc ccaacacggt tgccctgcca agccgtcagg
     1501 gtcatatcca caccgctttg cttcaaaaac tgatgaaaag cgtgaccaac aggattatct
     1561 ggtagggccg taaatagcct tgcttctaag ccaaaaccgc ctagagttct tgcaatatta
     1621 acttctgaac cgccgaaaaa aagctgagcg tcgcaggcat tggtcaaagg ctgaaactga
     1681 ttgggactaa cgcgtatcag cggttcacca accaataata acttagacat gtgatctcct
     1741 ttcctcgtca aaatggaatt actgttcaag tactgatttc aaataagtaa ctaattctga
     1801 cggctccgca ttggcaaaga agtactcttt aaaatgagcg ccagaaatcg tttctttgag
     1861 gaaatcttgg tcgatgtctt tgacaattgc caacaaatct ctgtaagtaa tttttttcag
     1921 atcacttaaa atacgtgcat tccgctgttc aggaatagct cgctctttag ggtaaccacc
     1981 acccattggt tctgcaaaga ggcgttcaaa tattaaggtc aaattcaact ctgctcccca
     2041 accaaaacct ttggcaaaag gaatggacaa ggcattacca ccattgattt gagaaaagag
     2101 ataagcttct gttggctcac tcgcaaaacc acaggtaacc cctgggaagc tgttcaaggc
     2161 caacatagct ccaacacctg ttccacaacc agtcaccaca aaatcggccg ccttagtggt
     2221 caataaaata gaagctaaga gtccattttg aacataggtc aactggcttt caccttctgt
     2281 gccatacatg ccatagttaa agacttgata gccgtgttta tctgtcactg aggtaagggc
     2341 atcgtaaata ataccatttt tagccgcttg gctattttca ttaattaatg caattttcat
     2401 ataaaatctc tttctagtgt gtgattaagg ttgttttcca atataagcta aaatgccacc
     2461 gtcgacataa aggatatggc cgttcacaaa attactggca tcgctagcta gaaaaacggc
     2521 tggaccagct aagtcttctg ttgtccccca acgtgctgct ggtgttttag caatgataaa
     2581 ttgatcaaaa ggatggcgac taccatctgc ttgtcgctct ctcaacggag ctgtttgggg
     2641 tgtcgcaatg tagccaggac caatcccatt acattgaatg ttggcttctc caaactcaga
     2701 ggctatattt ttggttaaca ttttcaagcc acctttagcc gccgcgtagg cactaactgt
     2761 ttcacgcccc aattcgctca tcatagaaca gatatttata attttaccat gtccctttgc
     2821 aatcattgat ggtaatacag cttttgacac aataaaagga gcatttaaat caatatcaat
     2881 aacttgacga aaatcttctg cggccatttc aagcatcggc gtacgcctaa taatacctgc
     2941 attgttgacc agaatatcaa taacacctac ttcatcttca atttgactaa ccatctgttg
     3001 gataccaact tcatcagtca cgtcacaaac atagccatgc gcttctatcc ctaactctcg
     3061 ataggcagcc aaccccttat caactaactc ttgtttgatg tcattaaaca caatagttgc
     3121 acccgcttga gcataggctt tggcaatttc aaaaccaata ccgtaagatg ctccggtaat
     3181 caaggcaatc ttaccttgta acgaaaacat attttccata ggattctcct ttttgttatt
     3241 aaattaagta aacctgttta ccaagactat aacaaagtaa actaattttg tcaagatgtt
     3301 tatgaaaacg gttcaaaatt taaaagagtg ctcttatcca aactgtcatt cttgtttagt
     3361 cttctcctat acttaatagc tattaagact tttctacatt aaatagtgac aaaaaagacc
     3421 atttttgatt cttttttagc acaaccgttc actttttctt tttcaaaaat ctttttaaaa
     3481 aagtattgac aaagaatttt gaaagcgcta caatattttt gagtaaaccg gtaaaccaaa
     3541 ggagatttat ttatgattgc tattatcgtt atgggacatg gacattttgc gagtggtatt
     3601 gtttcagcct tagaattgat cgcaggcaaa caagaaaagg tcactgctat tgattttact
     3661 gctgagatga ccgccgctga tgtgcaggac caattatctc gtgctttaat cccagaagag
     3721 gaaaccttag ttctttgtga ccttctagga ggtacccctt ttaaagtcgc tgcaacctta
     3781 atggaatctc ttccaaacac gacgtgtaat gttctttctg gccttaactt agccatgctg
     3841 attgaagcta gttttgcaag acaaacagca gcttcttttg acgacttagt atcaggactt
     3901 ataatatgtt ctaaagaagg cattgttgac tggaaaactc tttctcagca agaagacggt
     3961 gcgacagacg atgagttagg aggcatttaa tgacacgact tttaaaaacc attgctcttg
     4021 aacctatcaa gcagcctgaa cgatttacca aagaagattt cctaagccaa gaagacatta
     4081 ctcaggccct tgacttagcc ctcaaacaag ttcggcttaa catggactac ttcaaagaag
     4141 attttccaac gccagctacc aaagataacc aatacgccat tatggacaac acggaatgga
     4201 ccaatgcctt ttggactggc tgcctctggt tagcttacga atacagtgga gatgatgcca
     4261 ttaaagctct ggctcaagca aatgaccttt ctttcctaga tcgagtaaca agagacattg
     4321 agctagatca ccatgatttg ggctttcttt acaccccttc ttgcatggct gagtggaaac
     4381 ttttaaaaac acctgaggct agagaagcgg ctttgaaagc ggccgacaaa ttagtccaac
     4441 gctatcaaga caaaggcggc tttattcagg cctgggggga gctgggtaaa aaacaagatt
     4501 accgtttaat cattgattgt ctcctaaaca tccaattact cttttttgct agccaagaaa
     4561 ctggagacaa ccgttaccct gacatggcga tcaatcattt ctacgcctca gccaatcatg
     4621 tcatccgaga tgacgcctct gcttaccaca ccttctactt tgatccagaa acaggcgatc
     4681 ctgttaaagg ggtcacccga caaggttata gcgatgattc tgcttgggcc agaggccaag
     4741 cttggggcat ttatggtatt cctttaacct atcgtttcct aaaagaacct gaacttattc
     4801 aactcttcaa gggcatgacc cattatttct tgaaccgtct tcctaaagac caggtttcct
     4861 actgggattt aatttttgga gatggtagcg agcagtctcg tgatagttca gcaacagcca
     4921 ttgctgtgtg tggtattcat gaaatgttaa aaaccttacc agatcatgat cctgataaaa
     4981 aaacatatga agctgccatg cacagcatgc taagagcctt gattaaggat tatgctaaca
     5041 aagatctcaa tcctggtgct cctctactgc ttcacggagt ctattcttgg cactctggaa
     5101 aaggggtgga tgaaggaaat atctggggag actattacta ccttgaagct ctgcttcgtt
     5161 tctacaaaga ctggaaccct tactggtaac aaacaccgat aatcacattt aaaaaggaga
     5221 ttttatgaca caaccaaaca ttatcatgac gcgagttgat gagcgcttga ttcacggaca
     5281 ggggcaatta tgggtcaaat acctcaactg caacactgtt atcgtcgcta acgatgcggt
     5341 ttctgaagat aagattcaac aatccctcat gaaaacagtt atcccatcca gtattgccat
     5401 tcgttttttc tccattcaaa aagtgattga catcattcat aaagcaagtc ctgctcaaag
     5461 tatcttcatc gtggtcaaag atttgcaaga tgccaaacgc ctagttgaag ggggtgttcc
     5521 catcacagaa atcaatatcg gtaatattca taaaactgat gataaggttg cgattaccca
     5581 gtttatttcg ctgggagaaa cagacaagtc tgctattcgt tgcttagccc atgaccacca
     5641 agtggttttt aacaccaaaa caactccagc tggcaatagc gcctctgacg ttgacatctt
     5701 agattatatt taatgaataa aggagattga tatggatatc aatttgttac aggcactttt
     5761 aattggtctt tggacagcct tttgttttag cggaatgtta cttggcatct acaccaaccg
     5821 ttgtattatt ctgtcttttg gtgtagggat gatcttaggt gacttgccaa ctgcacttag
     5881 catgggagct atctccgaat tagcttatat gggatttgga gtgggtgccg gtggtaccgt
     5941 tcctcctaat cctatcggcc ctggtatctt tggtaccttg atggctatta ccagtgctgg
     6001 taaagtcacc ccagaagcgg ccctagcctt atcaacacca atcgcagttg ccatccaatt
     6061 tcttcagaca ttcgcctata ccgcttttgc tggggctcct gaaaccgcta aaaagcaatt
     6121 gcaaaaaggc aatataagag gcttcaaatt cgctgccaac ggcactatct gggctttcgc
     6181 ttttatcgga ttaggccttg gtttattagg tgccttgtca atggatactc tccttcactt
     6241 ggttgattat atcccacctg tcttgctcaa tggattgact gttgctggta aaatgttacc
     6301 tgctatcgga tttgccatga tcttatcagt tatggccaag aaggagttga ttccttttgt
     6361 actaattggt tatgtttgtg cagcctacct ccaaattcca accattggta tcgctattat
     6421 tggtatcatt ttcgccttga atgaatttta caacaaacct aaacaagtcg atgcaacaac
     6481 tgtccaagga ggccaacaag atgactggat ctaataaatt aaccaagaaa gattacctca
     6541 aaacagctct gagagctttc ttcctacaaa atgggtttaa ttacagtaat taccaaggta
     6601 tcggatacgc caatgtcatc tatcctgcct tgaaaaaaca ttttggcagt gataaaaagg
     6661 gcttatatca agctttagaa gataactgtg agttttacaa tactaaccct cacttccttc
     6721 ctttcatcac cagtctgcac ttggtcatgt tagaaaatga tcgtcctgaa gaagaaaccc
     6781 gaaacatcaa aatggcactt atggggcctt tagctggtat cggtgactcc ttatcacaat
     6841 tttgcttggc accactcttt tcaacgattg cagcttcctt agccagtgat ggtttagttt
     6901 taggtccaat tttcttcttc ctagccatga atattatcct aactgctatt aaaatcggtt
     6961 ctggtctcta tggctataaa gtcggcacaa gctttattga taaattaagt gaacaaatgg
     7021 cggttgtgtc gcgcatggca aatattgtcg gtgttaccgt tatcgctgga ttagctgcta
     7081 cttctgttaa aataactgtt cccattactt ttgcggctgg caaggtggat gcggccaata
     7141 cagcacaaaa atttgtgact attcaaggaa tgcttgataa aattgctcct gcacttttac
     7201 cagctctctt taccttactc atgtattacc ttattaaaaa caaaaaatgg acaacctaca
     7261 aattggttct cctcactgtt cttatcggta ttatcggtag ctggttaggt attttagctt
     7321 aatggttcac agagacctgt tatgagcata gggaaatagt tcctaagcac atgatgaggt
     7381 ctcttttctt ttggaaagga aatgtcctat gtctcttgcc ttcacacgct ttaaagaaac
     7441 agtcaatcca gacttttgca ggaattacct gctagactat cagactgaca gctatgctga
     7501 tcaaaaaaga atagctgacc tactcttaac caataccttt ttatttgaag ataattggga
     7561 tatggagccc tgccacatcc cttaccactt agatcccatt acttggcaag aggctgtcac
     7621 agatgatcct gagtggaatt ttatgttaaa tcgacagacc tatctccaaa aattaatcct
     7681 cgtctacctt gttgaaagag atgaacggta tctcctaaaa gccaaaagtt tcatcctcaa
     7741 ttggattgaa tcagctattc ctttagaccc taaggggtta gctactagaa ccttagatac
     7801 tggcatccgt tgttttgctt gggtcaaatg tttgatttac cttaatcttt ttaacgcctt
     7861 aaccaagcaa gaggaaagcc tcatcctagc ttccctggaa aaacaacttc aatttctcca
     7921 tgccaactac cttgataaat acagcctcag caattgggga atcttgcaaa ccactgcgat
     7981 tttattagct gatgcctact atggttctga ccttgatatt gctgcagcaa cagcttttgc
     8041 tagaaaagaa ttgacgcaac aaatcgcact ccaaattctc gaagacggca gtcaatttga
     8101 acaatcaacc atgtatcatg tggaagtact caaagccctt ctggaattaa ctgctctagt
     8161 ccctgattat cttccacagc ttagacctac tttgcttgcc atgagtgatt atctcctcaa
     8221 aatgactggc cctgaccaca aacagatccc cttaggagac agtgacttta ccgataccag
     8281 agatattcta acactagcag ccactatcct tgaagaacca cacttaaaag ctgctgcttt
     8341 tcccacactt gatatcgata gtcttctcct tctaggagaa aaaggtgtcc acacttttga
     8401 acagttaccc gtccaaacct taccaacttt tgcgcatcac tttgaacatt ccgggcacat
     8461 taccatcaat caagaaaatt attatctttt tttcaaaaat gggcccatcg gtagttccca
     8521 tacccatagc gaccaaaata gtctctgtct ctattataaa ggacaaccac ttttctgtga
     8581 tgccgggcga tacacttaca aagaagaacc attacgctat gccctaaaaa gcgctagcca
     8641 tcattctacc gccttcttaa aagaacaatt acctgagcag attgatagct cctgggctta
     8701 cctttcttac cccaaatcca attattgtca ccttcgacaa aatggacacg tttactttat
     8761 tgaaggtagc taccagacgc aattctctga taggaacaac taccaacatg accgacaaat
     8821 tctcatctta ccaccaggaa tgtttcttat cattgatacg atacaggctc aaggtaacca
     8881 ctgtcttgtc agtcaattta ttcttgataa tcacttagac gtaaagacag atcaccttag
     8941 tgacctcaga ctaatcagtg actgcccttt cactatcgaa gaaacgattt tgtcaaaaaa
     9001 atacaaccaa tacctgacta gtcacaaatt catcaaacga aaacccttta aagacaaggg
     9061 ttgcaccagt accctgttag tccctgacga taccaaagtt actcccttaa caccacttca
     9121 aacaggcaaa cgcaacccaa ttgagaccgc cctttcctgg catttaaaag gaaagcagtt
     9181 tgactacagt atctgcgttc tccaagagga cttaattaaa ggagaaaaac tagttctcct
     9241 aaatggtcat aaaatcagag gaaaggtggt tgttatcaac catatcacga atgagattat
     9301 tcgattgaaa cactaaatct tttacgagtc cttatgttat aatatgacta accattacta
     9361 tttttaagga gtgtatgcaa tgcagcgcaa agtaaccatc aaagatattg ctgaattagc
     9421 gaaaacttca aaaacaacag tttctttcta cttaaatggt aggtttgaca agatgtctga
     9481 agaaactaaa aaccgcatta aaagcacgat tgaagctacc aattataaac caagcattgc
     9541 tgccagaagt ctaaacgcca aatctactaa actcattggc gtggttattg gtgatattac
     9601 caatagtttt tccaatcaaa ttgttaaggg aattgaaagc aaagcccagg aatttggtta
     9661 ccaaattatc atcggaaaca gcaactacga cccttctcgc gaagatgaac tcattgaaaa
     9721 gatgctgaac ctcggagttg atggctttat cattcagcct acctctaatt tcaggaagta
     9781 ttctcgcatt atcgatatta aaaaaaagaa ggttgtcttt tttgatagtc aactttatga
     9841 gcatcgtacc aactgggtca aaactaataa ttatgatgct gtttatgata ccattcaaca
     9901 atgtatcgat aaaggttatg aacattttat catgatcacg ggtaacccca atttacttag
     9961 tactcgtatt gaacgcgctt cgggcttcat tgatgtgtta gaagctaatc atcttactca
    10021 tcaagaaatg attattgatg aaaatcagac ttcttcagag gcaattgctc aatttttgca
    10081 aggatcactg actaaaaaga gtttggtttt tgtccccaat tgttgggctc ttccaaaagt
    10141 atttacagct atgaagtctc ttaaatttaa tataccagag attggactag taggatttga
    10201 taacattgag tggacaaaat tttcttcacc aacactaaca actattatcc aacccgccta
    10261 tgaagaaggc gaacaggcta ctaagatttt aatcgatgat attgaaggtc acagtcaaga
    10321 agccaaacaa caaatttttg actgtcaagt caattggcaa gaatcaactt tttaatgtca
    10381 ggcataccaa tagtccttat cgttgtaacg attataaaaa aaggtttgag acaattgtct
    10441 caaacctttt agcgtctatt aaattgcttt ttcatcttga ccaagtgatc gttgaacagc
    10501 tttgacaagc tcaacaagaa caaccatcaa gagacttccg ataataacca ctaaccattg
    10561 tgtgatcgtc aagtgtgtca catggaagaa ttgattaaat ccaggaacaa caactgttgc
    10621 catcaatgct acaaaggcca ctggaataga atagttgaac aatttgtttt tgaacaaccc
    10681 aacagtaaag atggattggt aaacagattt cacgttgtag gcatgtacca actgaatcaa
    10741 accgagggtc acataagcca tggtcaaagc atctgcatgg acatcatgat agcttgtgtg
    10801 ttctgggaac atcaaggcaa aaccataaac cccaagaacc aaaatggttt ggaaggcacc
    10861 ttggtaaaga atggcttcct tcaccccacc atcaaagaag cttgacttac gtccacgagg
    10921 tttgtgcttc atcacaccag gctcagcagg ttcaacacca agagcaatag ctggtagggt
    10981 atcagttacc aagttaatcc aaagcaagtg aactggttga agcacatccc aaccaaacaa
    11041 ggttgcaagg aagatagtaa agacttctgc catgttagca gaaagtaaat attgaattgt
    11101 tttttgaata tttgagaaga ccttacgtcc ttcttcaacc gcaacgataa tggttgcaaa
    11161 gttatcatcg gcaagaacca tgtcagaagc tcccttagaa acctctgtac ctgtaattcc
    11221 cataccaata ccaatatctg ccgttttaag agatggtgca tcgttaaccc catcaccagt
    11281 catggcaaca actttgcctt cattttgcca agccttaacg atacgaactt tatgctcagg
    11341 agatacacgg gcatacacgg agtattgttt aaagactttt tggaactctt catctgataa
    11401 ttcgttgagt tcagcacctg taaagacatg atcaacgcca tcttcttcga taataccaag
    11461 acgtttcgca atagcttttg ctgtatcttg gtggtcacca gtaatcatga ttggacgaat
    11521 gccagcttcc ttagctactt taacagcctg tgctgcttca ggacgttcag ggtcaatcat
    11581 ccctaccaaa ccagagaaga caagattagc ctcaacaatt tctgtttcca gtgttggaag
    11641 agcatcagaa tatttgtaag ccatcatcaa gacacgaaga gcttgtttgg caagactctt
    11701 attggtatca agaatggttt ttttatcagc atccgtaata ggacgaattt gtccattttc
    11761 ttcaatctgt gtgacacgtt tcaataactg atctggtgca cctttaacag cgatgaaata
    11821 tttgccatca gcttgtttat gaatggttga catcaactta cggtcagaat caaatggtag
    11881 ttctgccaca cgaggttctg ccaccatagc ctcacgtacg tcaaaattgt ggtctaaccc
    11941 aaactgtacc aaagctgttt ctgttgggtc accaattaac ttccctgaag gatcaacttt
    12001 agtatcatta gcaaagttca tcacacgaag tgtcgtattg tcaaaggcaa tatcagctga
    12061 tgaactttgg agtgtcccgt tggtataaac tttttcaacg gtcatttgat tcattgtcaa
    12121 ggtacccgtt ttatcagaag caatgatttc agttgatcct agcgtttcaa cagctggtaa
    12181 tttacggata atggcgttac gtttagcaag tacttgagtt ccaagagaaa gcacaacagt
    12241 aacaatcgca ggaagtccct ctggaatagc tgcaacggca agcgctactg aagtcatcaa
    12301 gccttcaagc gggtgttgac cacgcaagaa gactccaaca gcaaaagtca ccgctgcaat
    12361 cacaaggatc gcataagtca aaatcttaga aaggttgtca aggttttgct tcaatggtgt
    12421 atctgtctca tcagcattag caagcatacc agcaatatgc ccaacctcgg tgtacatgcc
    12481 agtgttagtg ataaccccaa tcccacgacc atatgtcaca tttgagtttt gatatcccat
    12541 attgacgcga tcaccaatac cggcatcttc tgaaacagcc gttgacaagt ctttttcaac
    12601 cggtacagat tctccagtaa gcgctgcttc ttcgattttt aaggagttag cttccaaaag
    12661 acgtagatca gcagggacaa catctcccgc ttctagcaag acaatatctc cagggaccaa
    12721 ttctttagag tctatctcag tcacatgccc atcacggcga atacgagcca gtggacttga
    12781 cattgatttc aaggcttcaa tagcagcttc agcttgtcct tcttggtaaa caccaaaagc
    12841 agcatttagg ataacaacag ccaagataat aatggcgtct gtcaaacctt ccatcccctc
    12901 tgtaagaact gaaagaaggg ctgccacaat caaaataata atcatcaaat ccttaaattg
    12961 atcaaggaat ttcataaata aacttcgttt ttcgccttca tcaagctcat tacgaccata
    13021 ctctgctaag cgctcttttg cttgggcaga cgtaagacct tcgcgactgg tctctaattg
    13081 agccagtacg gtctcttcac tttgcgtgta aaacgcttca tgtctttgtt ctttagacaa
    13141 attagtacct ccttggtctt tttattttag ttattcaaat aaaaagacct gtttattttt
    13201 cttaaacaag tctcgctgtt taagattgta ccggaaaccg aaattccgta ttgacgatac
    13261 aatcacggct agccgtctgc tactcccttg atataataac cattatagta aaaacctgac
    13321 taaaaaacaa gaataatccc taatcagatg atttactgct atctttttag ttaatcccaa
    13381 ctcagctcaa gatgatagct tgcaaatagc atttctgttt gagcttgaag caaatgataa
    13441 tgaaggtcca atttttgaca agaacaatct aagtggtaat gactggtatc tgtcaaaaat
    13501 tgttgaaccc ccatcggcgt aggtagagca atcaggacct tcttgcccgc aaaaaatttc
    13561 atgattgact gagggttgga aaaacggctc atggtcagtt ctgtctcatt gtacttgatg
    13621 acaacctttt ccttgtcagt gttttgatag atgaggtact gatagtcccc tttttctcgc
    13681 cactcacaat catgaatttc ttgaataatc tctgtctcat ctccaaagcg aatatggttg
    13741 gttaactgta atttcataac tcttctttct attctaacta ttctaagctc gtctgtttat
    13801 tctatcaatt tctccttatt ttatcaattt ttgatataat aaaaattatg aatgaaaaag
    13861 tatttcgtga ccctgttcac aactatatcc acattgataa tccattaatt tatgacctta
    13921 tcaataccaa agaattccaa cgcttacgac gcatcaaaca agtccctaca acagctttca
    13981 ccttccacgg cgcagaacat agccgttttt ctcactgcct aggtgtttac gaaattgccc
    14041 gtcgggtgac agctattttt gaagagaaat acgctgatat ctggaataaa gatgaaagtc
    14101 tggtcactat gacggctgcc ctcttacatg acattggtca tggagcctat tctcatacct
    14161 ttgaggctct ttttcatacg gatcacgagg cctttactca ggagataatt accaatcctg
    14221 agacagaaat caatgccatt ttggtgcgtc atgctcctga ttttccagac aaagttgcca
    14281 gcgtgattaa tcatacctat cctaacaagc aagttgtgca actgatttcc agccagattg
    14341 actgcgaccg tatggattac cttttaagag attcttattt ttctgctgcc aattatggtc
    14401 agtttgattt aatgcgcatc ctacgtgtga ttcgacctgt tgaagatggc attgtctttg
    14461 aacatagtgg tatgcatgct gtcgaggact atattgtcag tcgttttcaa atgtacatgc
    14521 aggtctattt tcatcccgcc agccgtgctg ttgagctcat cttacaaaat cttctcaaaa
    14581 gagcccaaca cttatatcct gagcaacagg cttatttcca aaaaacagct cctggactca
    14641 tccccttctt tgaaaagaag gctaatttag cagattatat agccctagat gacggagtca
    14701 tgaatactta cttccaagtc tggatgacaa gcgaagacca tatcttatca gatttggcca
    14761 gtcgctttat taatcgaaaa attctgaagt ctgttacctt tgaccaagat tcccgaggag
    14821 agttagaacg cttgcgccaa ttggtcgaat cagttggctt tgatccagat tattacaccg
    14881 gtatccatat caacttcgac ctgccttatg atatttatcg accagagctc gaaaatccaa
    14941 gaactcagat tgaaatgatg caaaaagacg ggagccttgc tgaattatct cagttatctc
    15001 ctattgtcaa agcattaaca gggacaacct acggagaccg tcgcttttat tttccaaagg
    15061 aaatgttaga gctcgatgac ctctttgctc ctagcaagga aacttttatg agttatattt
    15121 ccaatgggca ttttcacttt agtcagtaaa gccctttaag attgcagaaa aagtcttttt
    15181 tgatactttt cttaggtggt gcttatggca gtgacttcct ttggaattct atgctttatt
    15241 tttgagccta gggtctcaaa aatcccgcgt aacgtcactg ccagtattga caccactttt
    15301 gccacagcaa aaagcagctg ttttggctca cgtcacacgc agaaatgaga aattagttgt
    15361 caaatcagca gagcagttca tgctttttga gcatcaaact gggtttgtct ccattctaag
    15421 attgaagaaa gagtactttc ttcaatcttt ttagttacta cagaggcgat tgagctaaac
    15481 taaaacgttt tctctcgttt ttccttaagt ttcctttccc atcccatgac aaaagcaccg
    15541 atttttagta agataggagg gagaacattt ttaagataaa ccacaaaaaa caaggaggat
    15601 gccatgtcta tcaaactggt tgctgtcgat attgatggca cattactcac tgatgaccgt
    15661 cgaattacag atgatgtttt tcaagctgtc caagaggcta aagcccaagg tgttcatgtg
    15721 gtgattgcta ctggccgacc tattgccgga gtgatttcat tactcgaaca attagaactc
    15781 aaccacaaag gaaaccacgt tatcactttt aatggtggct tggtgcaaga tgccgagacc
    15841 ggcgaagaaa ttgttaaaga attgatgact tacgacgatt atctggaaac ggaatttatg
    15901 agccgaaaat taggagttca catgcatgcc attaccaaag aaggtattta cactgccaat
    15961 cggaatattg gaaaatacac agttcatgaa tcgactttgg taaacatgcc tattttctat
    16021 cgcactcccg aagaaatgac caataaagaa atcatcaaaa tgatgatgat tgatgaacct
    16081 gaccttttgg acgcggccat caagcaaatt cctcaacatt tctttgataa gtacaccatc
    16141 gtcaagtcaa ccccttttta tttagagttc atgccaaaaa ccgtcagcaa gggcaatgcc
    16201 atcaaacact tagctaaaaa actcgggcta gatatgtcac aaaccatggc catcggggat
    16261 gctgaaaatg atcgtgctat gttagaagtc gttgccaacc ctgttgtcat ggaaaatggg
    16321 gttcctgaat tgaaaaaaat agccaaatac atcaccaaat ccaacaatga cagtggtgtg
    16381 gcacatgcta ttcgcaagtg ggtattaaac taatgtatac atataaaatc ggaatatctc
    16441 ctgaagaaca tgatcagttt gtcttagcac agccacaagc tggcttactt caaagttcaa
    16501 agtggggaaa agtcaaagat aactggaaac acgaacgcat cagcttttat gaaaatgggg
    16561 tacaagttgc tgctgctgct tgtttaatca gaaaacttcc tcttggtttc actatgattt
    16621 atatcccacg tgggccaatc atggactacg ccaattttga actccttgat ttcgttatta
    16681 aaacgctcaa aactttcgga aaaagcaaac gagcgctttt tgtcaaaatt gatccaagtt
    16741 tagtcatcaa acaaacttta gaaggaaaag aaagcaaaga aaatgatgtg gccctctcta
    16801 ttattgcttt tctaaaaaaa ctcggtgtcg aatggtctgg cagaacaaaa gaactcgaag
    16861 acactattca accaagaata caagccaaca tctacgccaa ggattttgac tttgacagcc
    16921 tgcccaaaaa agccaagcaa tccattcgta ccgctaccaa taaaggtgtt aatgtcacga
    16981 ttggtggcag tgaattgcta gacgactttt cagccttgat gaaaaaaacc gagaatcgaa
    17041 aaggcattat ccttcgaggc aaatcctatt atcaaaaatt actagatatt tatgctggtc
    17101 agtcctacat tactatggct agtttagact tacctgaaca aaaaaaaacc ttgatccaac
    17161 agttggacaa agccttagca gaacaagtac gattaacaga taagagtaaa cctagcaaag
    17221 tagcagaaaa tcaaaaaacc attgcacggc tacaaaaaga tttaaccatt ttaagcgagc
    17281 agctcgctgc aggcaaaacc cgcatcccac tagccgccac cctaaccttg atttatggcg
    17341 agacttctga aaatctttat gcgggaatgg acgatgatta tcgcaattat caagctcctc
    17401 ttttaacctg gtatgaaacg gctaaagaag cttttaaaag aggctgccgc tggcataatt
    17461 taggaggcgt ggaaaatcaa caagatggtg gactctatca tttcaaagct agactcaacc
    17521 ccaccatcga agaatttgca ggtgaattca acattcctgt cggccttgtt tcaagcttag
    17581 ccatcttgac ctataactta agaaaaaaat taaggagcac aactaatggc actaattgaa
    17641 atttcacaag aacaatttga ccattactgc cacagtctcg tccatcattc ctttatccaa
    17701 acctcagaaa tggccagctt gatggctaaa agaggggcaa aaccacaatt tcttggactt
    17761 gaaaaagatg gtgaactaaa ggttgctgct atggtcttta gccaaaaagt ggctggaggt
    17821 tggcgtatgg aactcaatgc aggccctaat accaatcatc ccgaagaatt agaacatttt
    17881 tacactcaac ttaaagacta tgccaaacaa aaagacgtta tcgaactcat tctaaaacct
    17941 tacgacaatt atcagtcatt tgatactgat ggcatcccta tttctcatcc aaacactgac
    18001 cttattagcc ttttaacggc gttaggatat aaacatgacg gtttaaaaac aggctatcct
    18061 gagggtgaac ctgtttggca ttacgttaaa aagttagaag gaattgattc ctcacattta
    18121 acccattcct tcagcaaaaa aggaaaagct ttaataaaaa aagctaatac ttttggtatt
    18181 aaattacgtc aactgaaacg agatgagtta caccatttca aagaaatcac tgaagcaacc
    18241 tctgatcgta gagattacct tgacaaatca ttatcttact atcaggactt ttatgatagt
    18301 tttggagact cttgtgaatt tatggttgcg actcttaatt ttgaagatta ccttaataac
    18361 cttaaacaaa gacaactaca actagcaacc agcatcaaca aagttaaagg agaccttggc
    18421 aaaaatcctc attctgaaaa aaagcaaaat agattaaaag aactatccag ccaattcgaa
    18481 acctttcagg taagaatttc tgaagctctc cattttcttg aagagtacgg aactaaagat
    18541 gtgttccttg cgggaagtct ttttatctat accgaacaag aggccgtcta cctcttttca
    18601 ggatcttacc ccaaatttaa taaattttat agcccagctc tattacaaga gcatgctatg
    18661 ctcaaggcaa ttcacaaggg gatcaaacaa tataattttc ttggtattac tggaaagttt
    18721 gatggttcag acggtgtctt gcgctttaaa cagaatttca atggctttat tttgcaaaaa
    18781 ccagggacat ttaaatgtta tccatttcca atcaaatatc atttcattag attggcaaaa
    18841 aagctattaa accgctatta ggctaatgca tttgcctgta gaatcacctt tattctctca
    18901 tagtaagagt taagtcatct aaaaaagaaa aggatttaac cttttctttt ttagatgact
    18961 tagttaagga aatcaagcaa agcaaggaag ctatcagctt caagtgatgc accacctaca
    19021 agggcaccgt caacatctgg acaagccatg taatctttaa cgttttcagg ttttacagaa
    19081 ccaccgtatt gaacgcgaac tttatcagca acttcttgcc caaagtctgc tgctaccacg
    19141 tcacgaacag ctttacacat gttttgagca tcatcttgag tagctgattt accagtacca
    19201 atagcccaga ttggctcata agctaaaaca agtgaagcta cttgctctgc tgaaagacct
    19261 gcaagtgctg ctgaaacttg cgcacctaca aactcaacgg ctttaccagc ttcataagtt
    19321 tcaagagact caccacaaca aacaattgga gttaaaccgt tagcaaagat tgctttggct
    19381 tttttattaa tgtcttcatc tgtttcatgg aagtaatcac ggcgttctga gtgaccaatc
    19441 acaacatagt cagctcccat ttcagcaagc acttttggtg atgtttcacc agtgaatgcc
    19501 cctgtgtttt caaagtagca gttttgtgca gcaactttaa gaactgaatc tttagcagct
    19561 tcaatagtgg ttaccaaatc aacagctggg gctgctacgg caacatcaac aaggtcagtt
    19621 gaaggtaatt tgcttgctac agcctcaacg aatgcttttg cttcttgagg gtttttgttc
    19681 attttccagt taccagcaat aattggttta cgtaacatat cacatacctc ttcttttttt
    19741 atttatatct tctattttag cacaaatgac cgacgattaa aaggaattgc ttatatttta
    19801 ggaatatttt cacaaataaa aactcttttt ctgaaagctt ttcattctta tttatcagca
    19861 ttttatggag tttaagatag caaaaagccc ccgaattggg ggcttcttca aacttaagtt
    19921 gtctgactta aattgctatt ctgggaactt aattaaaatt aagcttcgat ttctgaaacg
    19981 atacctgaac caacagtacg tccaccttca cgaattgaga aagtagtacc ttgttctacg
    20041 gcgattgggt ggatcaactc aacgttgatt gttacgttat caccaggcat aaccatttct
    20101 gtacctgctg gaagttcgat tgaacctgtt acgtcagttg tacggaagta gaattgtgga
    20161 cggtagttgt tgaagaatgg agtgtgacgt ccaccttcgt ctttagaaag gatatatact
    20221 tcacctttaa atttagtgtg tgggttgatt gaacctggtt tagcaataac ttgaccacgt
    20281 tcgatttcgt cacgttgaac accacgaaga aggataccta cgttgtctcc tgcaagacct
    20341 tcgtcaagtt gtttacggaa catttcaaca ccagtaacaa cagctttttt agtttcttct
    20401 ttgataccaa cgatttcgat ttcgtcgtta acacgaacag taccacggtc gatacgtcct
    20461 gaagcaactg taccacgacc tgtgattgag aatacatctt cgactggaag aagcaatggt
    20521 ttgtcagtgt cgcgttctgg ttctggaatg tatgaatcaa cagtatccat caattccatg
    20581 atgatgtctt caaatttagt gtcgccttca agagctttaa gagctgaacc ttgaataact
    20641 ggaaggtcat cacctgggaa atcgtattct gaaagaaggt cacgaatttc catctcaact
    20701 aattcaagca actcttcgtc atcaacaagg tcaactttgt tcatgaacac gataaggtgt
    20761 ttaacaccaa cctgacgtga aagaaggatg tgctcacgag tttgtggcat tggtccatct
    20821 gttgaagcaa ctacaaggat agctccgtcc atttgagcgg caccagtgat catgttttta
    20881 acgtagtccg cgtgtcctgg agcgtcgatg tgcgcatagt gacgagttgc agtttcgtac
    20941 tcaacgtgtg cagtgttgat agtgattccg cgttcgcgtt cttctggagc agcatcgata
    21001 gaagcgtaat cttttggttg gttaactgat gaaggcaagc gacgtgccaa tacagttgtg
    21061 attgcagctg ttaaagtagt tttaccatgg tcaacgtgtc cgattgtacc aatgttaacg
    21121 tggggtttac tacgatcgta tttttctttt gccattttag gaaaagcctc caataaaata
    21181 tattttatag atagacggta ggccatacag tctaacttta ccttactatt gtagcaaatt
    21241 acaatgaaaa tgcaagtatt ttatgttttt tgccatttct ttattttttt aaatag
//
LOCUS       DAUELZ010000018        20975 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.018, whole genome shotgun sequence.
ACCESSION   DAUELZ010000018 DAUELZ010000000
VERSION     DAUELZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20975
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.018"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(170..1306)
                     /gene="dnaJ"
                     /locus_tag="VHI09_000550"
     CDS             complement(170..1306)
                     /gene="dnaJ"
                     /locus_tag="VHI09_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEQ6120451.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYSLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            complement(1587..3413)
                     /gene="dnaK"
                     /locus_tag="VHI09_000551"
     CDS             complement(1587..3413)
                     /gene="dnaK"
                     /locus_tag="VHI09_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEQ6120452.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAAAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            complement(3594..4166)
                     /gene="grpE"
                     /locus_tag="VHI09_000552"
     CDS             complement(3594..4166)
                     /gene="grpE"
                     /locus_tag="VHI09_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEQ6120453.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            complement(4169..5203)
                     /gene="hrcA"
                     /locus_tag="VHI09_000553"
     CDS             complement(4169..5203)
                     /gene="hrcA"
                     /locus_tag="VHI09_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEQ6120454.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKTFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDSSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            complement(5338..5907)
                     /locus_tag="VHI09_000554"
     CDS             complement(5338..5907)
                     /locus_tag="VHI09_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ6120455.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPSAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQAIESNDLTNYD"
     gene            complement(5904..6647)
                     /locus_tag="VHI09_000555"
     CDS             complement(5904..6647)
                     /locus_tag="VHI09_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEQ6120456.1"
                     /translation="MKHNHFKLLLQTLVVLLFVGGIYLFIKPEKQATKTPSSQQASSQ
                     KVVKNSKESTGLPKVSPKDWELILVNRDHMTEELSPELVDVNGVSVDKRIEQATADFL
                     AAAQAIDPQEHLISGYRSVAYQAELYQSYIKKEMANDPTLTQEAAEALVQTYLQPPGA
                     SEHHTGLAIDMSTVDTLNASDPSVSKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            complement(6637..7344)
                     /locus_tag="VHI09_000556"
     CDS             complement(6637..7344)
                     /locus_tag="VHI09_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ6120457.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESILEVDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFLWLIDPDRDKQYIDNGSVTVVEFDDGQFTIKTIGDM
                     SYRYRGREIIEAMSDEA"
     gene            complement(7736..7975)
                     /locus_tag="VHI09_000557"
     CDS             complement(7736..7975)
                     /locus_tag="VHI09_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ6120458.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            complement(8194..8409)
                     /locus_tag="VHI09_000558"
     CDS             complement(8194..8409)
                     /locus_tag="VHI09_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120459.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTVYKKTIIYAKI
                     RLTGIALEPSGVSDVYLMNHHVCLHYY"
     gene            complement(8406..9845)
                     /gene="gatB"
                     /locus_tag="VHI09_000559"
     CDS             complement(8406..9845)
                     /gene="gatB"
                     /locus_tag="VHI09_000559"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEQ6120460.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIQAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIDIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKALSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIRLTPDNLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            complement(9845..11311)
                     /gene="gatA"
                     /locus_tag="VHI09_000560"
     CDS             complement(9845..11311)
                     /gene="gatA"
                     /locus_tag="VHI09_000560"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEQ6120461.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFI
                     TVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIEDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYTEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            complement(11311..11613)
                     /gene="gatC"
                     /locus_tag="VHI09_000561"
     CDS             complement(11311..11613)
                     /gene="gatC"
                     /locus_tag="VHI09_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEQ6120462.1"
                     /translation="MKISEEEVRHVAKLSKLSFSESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDVAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            complement(11845..12184)
                     /locus_tag="VHI09_000562"
                     /pseudo
     CDS             complement(11845..12184)
                     /locus_tag="VHI09_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dikinase"
     gene            complement(12628..13182)
                     /locus_tag="VHI09_000563"
     CDS             complement(12628..13182)
                     /locus_tag="VHI09_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEQ6120463.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSYMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            complement(13329..14111)
                     /gene="codY"
                     /locus_tag="VHI09_000564"
     CDS             complement(13329..14111)
                     /gene="codY"
                     /locus_tag="VHI09_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEQ6120464.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDDEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            complement(14328..15542)
                     /locus_tag="VHI09_000565"
     CDS             complement(14328..15542)
                     /locus_tag="VHI09_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ6120465.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISMSLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCNEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDGK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            15773..16225
                     /locus_tag="VHI09_000566"
     CDS             15773..16225
                     /locus_tag="VHI09_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEQ6120466.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNTKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            complement(16347..17735)
                     /locus_tag="VHI09_000567"
     CDS             complement(16347..17735)
                     /locus_tag="VHI09_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ6120467.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGIAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDIEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            17807..18772
                     /locus_tag="VHI09_000568"
     CDS             17807..18772
                     /locus_tag="VHI09_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEQ6120468.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAVQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNGLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            complement(19121..19825)
                     /locus_tag="VHI09_000569"
     CDS             complement(19121..19825)
                     /locus_tag="VHI09_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6120469.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVT
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(19838..20740)
                     /locus_tag="VHI09_000570"
     CDS             complement(19838..20740)
                     /locus_tag="VHI09_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120470.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLTD
                     AKERIHEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
BASE COUNT         6408 a         4417 c         3624 g         6526 t
ORIGIN      
        1 aaaatcattt gataatcaaa tcaattacct aattatgata tactaaataa accattttga
       61 caagagaaaa aaacgatatc tataaaagaa aacgttttct ctaactgttt tgaaataaaa
      121 aaacgtataa cttcgggaaa attatcctga gattatacgt ttgacatctt taaatatcct
      181 ctaaggcatc tttcacttta tcaaaaaaac cttttttctt tggatgtaac attttttcac
      241 cactcgcttc tgcaaatgct tgcaaggctt ccctttgagc gtcattgagt ttggttgggg
      301 tcacaatatt aacggtaaca tgttgatccc cttgacctcc accacggagt tttggtgctc
      361 ctttgccttt gaggcggaag gttttacctg tttgtgtccc agcagggata gccatctcaa
      421 catcgccatg aaccgttgga atttcaacag tatctcctaa ggctgcttga gtaaaagaaa
      481 tatctaagct gtagtaaata gtggaaccat tgcgttcaaa ttgcttactt ggtaagacat
      541 tcaaaataac aaataaatca ccataaggtc cgccattaaa gccagcttct ccttgacctt
      601 gtaaacggat ttgctgacca gtctctacac cagctggaat cttaacagac actttatgag
      661 cttgcttttc atgaccagta ccgtgacagg tttgacaggg ttctttaatt tccttaccac
      721 taccatgaca aatatcgcag gtgacttgtc gacgcatcat accaagtggt gtttgggtgt
      781 caattgtcat tacacctgaa ccatggcact tacgacaagt tactggagcg gtaccaggtt
      841 ttgcacctga gcctaaacaa gtgccgcagg ttgcttcgcg attatagcta acttcttttt
      901 caacaccaaa tacagcctct tcaaaagata aattaacacg atattgcaaa tcgtcacctt
      961 gacgaggggc atttgggttt cggcttccac cgccaccaaa gaagcttgag aagatatctt
     1021 caaaaccacc aaagccacct ccgtcaaaac caccaaagcc gcctgctccg ccaccaaagc
     1081 cgccttgagc acctgcagcg ccgtattgat cataagcagc acgcttttgt gaatcactta
     1141 aggtttcgta agcttcttga acatccttat acttttgctc cgctccagct tctttattga
     1201 tatctggatg atatttctta gacatttttc gataagcttt tttgatatcg tcctgagaag
     1261 cgtccttaga aacccccaga cgatcgtaat attctgtatt gttcataatg taagatacca
     1321 agccgtaaaa cgactcaatg aaaaatagga aatccgacaa agatactctc gtatctagga
     1381 ggtatttctc ttttttcgca gagtcgtagg cgtgttcaaa tccgcaaaag agtctttaca
     1441 cgctttagcc tgtgttcgaa taatcgaact tgtattcctt tcttaaaata ttttgacgtc
     1501 agcattttta cattttattt agccaaaaac agaggtttgg tacaacctct gaattcggat
     1561 attaatgtta ctagataact aaatcattac ttttctgtaa attcgccatc tacaacatca
     1621 tcattatttg ctgaatcatt agcttgtgca ccttctgcac cttgtgctgc ttgttgagct
     1681 gctgcagctt gctcgtacat tttaacagcc aaagcttgcg ctttttcatt taatgcttca
     1741 agtttagctt tcatgtcgtc aaggttgcca gattcttgcg cagcttttaa ctcgtcaaga
     1801 gctgattgcg ctgcatcacg ttctgtatca aagcctttac cttcagtttc tttgattgtt
     1861 ttttcagtag caaagatagc ttggtcaact tcgtttttaa ggtcaacttc ttctttacgt
     1921 ttcgcatcgg cttcggcatt agcttcagca tctttcatca tgcgatcaat ttcttcttca
     1981 gaaagtccgt cgtttgattt gataacgatg tgttgttcct tttgcgtacc aaggtcctta
     2041 gcttttacag aaacaatacc gtttttatcg atatcaaatg ttacttcaat ttgtgggatt
     2101 ccacgaggtg cagctgggat atcagtcaat tggaagcgac caagagtctt gttatctgct
     2161 gccattgggc gttcaccttg aagaacgtgg atatcaacgg ctggttggtt gtctgctgct
     2221 gttgagaaga cttgtgattt agatgttggg atagttgtat tgcggtcgat caatttagtg
     2281 aagacaccac ccattgtttc aataccaagt gacaatggtg ttacgtcaag aaggacaacg
     2341 tctttcacat ccccagtgat aacaccacct tggatagcag cacccatagc aaccacttca
     2401 tcagggttta cagatttatt tggttcttta ccagtttcag cttttacagc ttcgacaact
     2461 gctgggatac gagttgatcc accaacaagg ataacttcat caatttctga caatgacaat
     2521 cctgcatctg aaagagcttg acgaactgga gttttcgtac gttcaacaag gtcacgagtg
     2581 agatcgtcaa atttagcacg agataagctc atctccaagt gaagaggacc agcagaacca
     2641 gcagtgatga acggtaatga aatttgagtt tgagttacac ctgacaaatc ttttttagct
     2701 ttttcagctg catctttcaa gcgttgaaga gccattttgt cttgagaaag atcaatacca
     2761 ttttctttct taaattcagc caccaagaaa tcaataattt tctggtcaaa gtcgtcacca
     2821 ccaagtttgt tatcacctgc tgttgcaaga acgtcgaaga caccatcacc taattcaagg
     2881 attgatacgt caaatgtacc accaccaagg tcaaaaacta agattttttc atccttgtca
     2941 gtcttgtcca taccataagc aagtgcggct gctgttggtt cgttaacgat acgttctact
     3001 tcaagacctg caattttacc agcgtcttta gttgcctgac gttgtgcatc gttgaagtaa
     3061 gctggaacag taataactgc tttttctact ttttctccaa gatagtcttc agcataacct
     3121 ttaaggtatt gaagaatcat tgctgaaatt tcttgaggag tatattcttt accatttgca
     3181 gaaacttttt cagaagttcc cattttagat ttaatagaga ttactgtttc tgggtttgtc
     3241 actgcttgac gttttgcagc atcacccacg ataatttcac catttttgaa tgatactact
     3301 gaaggagttg tacgattgcc ttctgggtta gcaatgattt ttgattcagt cccttcaaga
     3361 actgctactg ctgagtttgt tgtacctaag tcaataccaa taattttaga catgttaata
     3421 cctcttattt tataatactt tattactttt tatttcaatt tatcttagta agcactcgaa
     3481 ttattttact tccaattcta agcaatcact atagtttaat cagcaagcga aataacttct
     3541 aacttgctat ctagtttact gtcattacgg acaaaaacga aaactcattt accttagtta
     3601 tagacaacta ccatcgctgg tcttagcaga cgctcgtgca atttatatcc cttttgaaag
     3661 acttctgcaa tgctatcggc tggatgttca tcatcggctg ggagggtctg aactgccata
     3721 tgaaagttgt ggtcaaatga gtcaacttct acttcttcta cgccttcttc ctttagtgct
     3781 tgaataaggc tatcacgagt catttctaaa ccttttttaa catcatctgt caacccttca
     3841 acagccaagg cacgttctag attgtcaagc gatggtaaga ttgcttttgc cagatcctga
     3901 gaacgatagc gttgaagttg ttgacgttct tcactagagc gacgctgtat attctgcatt
     3961 tctgcatgag ctcgcaagta tttattttca aactcatcag cacgttcgtt tgcaagttct
     4021 aactctgatt tttcaggagt ttcttcaata acttcctcaa ctgtctcttc agtttcagtg
     4081 gcttccactt cttcttgtaa atcgtctttt ttaatttctt ctgacactga gtgcctcctt
     4141 ttaaataact tattgaatac tgccattatc agtggacttc ataatggtta gaatttaggt
     4201 atcgataata atcacctaat ttagcagcca atacctttcc aataatgttt accaaactaa
     4261 cagacctacg ataatccata tcaataggac caatcaaact caggagtcca aacccacggt
     4321 aaggaatcaa aaatttgtga gtcaaaactg acacatctgc aagtgcggct tcttgactat
     4381 ctgccacttg gacagaagcc atctcacctt ctttcagact ttgcctcaaa gaaattgcta
     4441 cttgctgttc attatccaaa aactgatacg ttgatagatc tgaatacgtt aaagaattta
     4501 ccttaccagc aacaaatacc gtttccaaaa acagctctga aaaaacataa tcaaatagtt
     4561 gtaaaacatt atccgtagtc acaaaatact tttgaacgat ctgaggaatt tctgtcctca
     4621 acttataatg aatatctatg acactactat ccagcaagcg ctcctctacg atagctttaa
     4681 atgctatcaa gtcacgtgtc aaaaagtttc ttggaatagc aaactgcact gtcactggct
     4741 tagattcatc taaagtcatg actgctagag catcatggtt gctaagctga acaacatcaa
     4801 accctgttaa acgttgacga gctggctcca catctaaaat aacggatgta tagcccgtca
     4861 tctccgctaa aatgtgacta gctttttgaa gcatgtcttc taatttgaat gcttcaaaat
     4921 caaaagtttt tatgacatga taaatatctt gctcatcaat actatctagc ctgagggagt
     4981 gctctacaaa atacttaaag ccagctggac ttggcatccg tccactagaa gtgtgcgctt
     5041 tttccaacaa tcccaacttc tctagttttg ccatatcatt acgaatagtg gcgctactag
     5101 aatcaatcgt tctctgtaaa gctttagacc ctactggttc atgagtttgt gtaaatagct
     5161 caacgattaa attcaaaata tcattttgac gttgtgtaat cacaaaccaa ccactccctt
     5221 tcgtttagca ctctatttag tagactgcta atttataatt ctattatact ctttatggga
     5281 gacatgtcaa gaaaaaaact caaaaaaatt agcactcttt tataaagagt gctaatttca
     5341 gtcatagttt gttaaatcgt tactttcaat ggcctgaatc agtcgcctag cataggtttt
     5401 atcagtataa tgcaaatact tttgtaaact tttagctggt gctttatacc ctttttctgt
     5461 agcaagatta cgatacaaac gcttatcgac ttcttttccc tgccgaagca ttcgtaaata
     5521 atctctaatt gcactagcgt catctttata aatagcatat ctcacgtttt ggttggtttg
     5581 tttaggggac tttagggtaa tggccgcttg tcctggagtt gctttcttgc taaaaagatt
     5641 atgatactta gacgctagta atgtttttcc atcatgggtt tccaaaagaa tttgagcaat
     5701 caaaatagat ggtctaatgc cataatattt tgccgatggt ttaacttctt tacccatttt
     5761 tgtaataaat tgtttttggc tgtagttcac ccttacttct ttatctgcac tagctatcct
     5821 ttggctactc agtaagggac aaacaatcag caataaaaag caagctagaa gcgttaaaaa
     5881 atacggaaac tttagtcgtt ttctcatcgt ttctccttta aagcagcaat gtactcttcc
     5941 aacgttaggt tgtgctgagc catataacga gcagacgcct tgcctacata gcggtaatgc
     6001 caatcctcat aatcaacccc tgtactagtc ttttttcctt ctggaaagcg caagacaaac
     6061 ccataatcag gtgcaatttt ttgaactgct ttagataccg atgggtcact ggcatttaaa
     6121 gtatctacag tgctcatgtc aatagcaaga ccagtgtgat gttcactagc ccctggcggc
     6181 tgtaaatagg tttgcaccaa agcctccgca gcttcttgtg tcaatgttgg atcgttagcc
     6241 atctcttttt taatataaga ttggtaaagc tctgcttggt aagctacgga gcgatagcct
     6301 gaaataaggt gctcttgtgg gtcaatagcc tgagctgccg ctaaaaaatc agcagtagcc
     6361 tgttcaatac gcttgtccac cgacacccca ttcacatcaa ccaattctgg gctcaattcc
     6421 tcagtcatat ggtcccgatt cactaaaatc aattcccaat cttttggaga gactttagga
     6481 agccctgtgg actctttgct atttttgacc actttttgac tgcttgcctg ttgacttgaa
     6541 ggcgtcttgg tggcctgctt ctctggctta ataaagaggt aaatacctcc gacaaagagc
     6601 aggacaacca gtgtttgcaa taataattta aaatgattat gcttcatctg acattgcttc
     6661 tattatttca cggccacggt agcgataact catatctcca atggttttga tggtaaattg
     6721 cccatcatca aattcaacaa ctgtaacgct cccattatca atatactgtt tgtctcgatc
     6781 tggatcaatc aaccacagaa acgttccaat tgtcatacca tggctaacca caagagcatt
     6841 tccgccgcca gcattttgaa tagacaaggc aatagcttca aacccctccc aaatgcgctt
     6901 acgaagaact tcccatggtt ctgcccaatt agcggtatct acttccaaga tgctttctgc
     6961 cagttcagaa tagggaacat cacgtaggtt atcacgattt tcaaaggctt ttgttcttgg
     7021 aaggacacct agaaataatt ctgaatcata agtcccttct agactcccaa aacaccactc
     7081 acgaatacga ttatctttag tataaggcaa aaattcgttt tctgattctc ttaaaataat
     7141 ttctattgtc tgcatggttc gacctgaatc acttgaaaac gcagccttaa atggtatagt
     7201 tgcatctttt aaacctaagc caagttctcg aataccttct tctccttttt ttgtaagagg
     7261 tgtgtcactc caaccctgag cgcgaccaat ggtattaaac atcgtttttc catgtctggc
     7321 aatatacaac cttgtttttg tcatttcaac ctcctaaact gtcttattaa ttattatagc
     7381 atttaaagaa aacacttgca aaacacttcc tgatagctat ttgagatcaa atagaccact
     7441 gacttattgt ctattttatt aaaaataatg ctattgctac aattcaaatc atcacataaa
     7501 tttccaaacg attacatttg tgcgtttcgc tcaaatatat ataacaacag acccacacta
     7561 caactaaact gacgttacaa aatgatgtca aaacagtaac aaaatctaac tatttgtcaa
     7621 gaaaatgata ttaaattttc acacaactat cttataatga ggatgcaacc aataaaaaga
     7681 taagacattt tatgttgcaa aaaaggctgc ttggaaactt ccatgcagcc tttttttaat
     7741 acctttttcc aaaaatagct gaaacaagta caattacaat tgccgctccc aaaatggacg
     7801 ggaaaatagc catacctgcc aactgaattc cccaatcccc aaaaagcctt tgcccaacag
     7861 ctgaccctat gagacctgcg ataatattag taaaacaacc catggactgg cttttttcag
     7921 tcattttacc tgctatcatg ccgataaagg cacctacaat taaagaccat atcatcgtta
     7981 acacctcctt tcttgtaaaa tccttttaga agaactgcta tccattaaat gttcttaact
     8041 ttagttgatt cctttacctt cttctttagt aaatgtttcc gtgcctgagt aaaatagaaa
     8101 aaacaattcc ttctattaag gtagatagac cgagaacgac tagaagtctt cacacactaa
     8161 ataacttaac aaactttatc acacagctaa tcgctaataa tagtgtagac aaacgtgatg
     8221 gttcattaaa tacacgtcag aaactcctga aggttctaaa gcaatacctg tcagtcttat
     8281 tttagcatag atgatggtct ttttatagac ggtcactaac atgacataca aaaaggcagc
     8341 ctgtgaaaga ccgaccgcct ttttttcaga gacaaaatac tttaacttat tgctatcagc
     8401 aactatcaat ctcgcaattt ttgcaattct tgtgccaata attgttgagc gacttgtgga
     8461 ttagcttgac ctttggttgc cttcatcaag aagcctgtga aagccttatc agcattacgt
     8521 ttgcctgatt tgaaatcagc cactgcagct tcattatcag caaagacttg gtgaataatt
     8581 ggtaccaaaa cagcgggatc agaaatctga acaagtcctg ccttttcgac gtaggcgcga
     8641 gctgagccac cgtttttagc caaatgcaca aagaccttct tagccatttt agaagagatt
     8701 gtaccatcag caataatagc aatcatttcc actaagttgt caggcgtcaa tctaatttct
     8761 tcaatggttt tgccttctgc atttaagaat tgggcaactt ccccttgcaa ccagttagag
     8821 acttgcttgg catcgccacc aagacttact gcagtttcaa agaaatctga caaggctttg
     8881 gtagctgtta gttggctagc atcataagct gagaggccaa gttcctcttc gtatttagca
     8941 cgacgctgag ctgggaattg aggtaattga gcacgcatct cgtcaatcca agcatcatca
     9001 atctcatata atggcaggtc tggttctggg aaataacgat agtccgctgc cccttctttg
     9061 acccgcatca aaatagtccc cttattcgct tcgtcatagc ggcgcgtttc ttggcgtatc
     9121 acaccacctg aacgcaagag tttggcttga cgttccacct caaattcaag ccctttacgg
     9181 acatttgaga aggagttcaa gtttttcaat tcggtctttg ttccaaattg ttcttgcccg
     9241 taaggacgaa gggaaatatt ggcatccacc cgcatagagc cttcttccat cttgacgtca
     9301 gaaatgcctg tgtactggat gatttctttc agagctgtca aataagcata ggcttcttca
     9361 ggagaacgca tgtcggcttc tgacacaatc tcaatcaaag gaactccttg gcggttaagg
     9421 tccacataag aataaccatc tgtgccgtga gtgtttttac cagcatcttc ttccaagtgt
     9481 gcgcgttcaa tacgaatttt cttggttgaa ccgtcttcta gtttgatatc aatccagccg
     9541 ttgtagccaa tcggctcatc aaactgtgaa atttggtaag ctttaggatt gtctgggtag
     9601 aaataatttt tgcggtcgaa atgcatctct ttatggatgt ccatattgag ggcaagcgca
     9661 gcttgtatgc ccgcatcaat cacccctttg ttcatgactg gtaagacccc tgggaaagac
     9721 caatcaatca cattcgtgtt ggcatttggg tcctcaccaa agtgagctga cgaaggtgag
     9781 aaaattttgg aattggtatt taattccaca tggacttcaa gcccaataat ggtttcaaaa
     9841 ttcattactt gtcacctcca aaaataatcg gttgttgctt gtgataatct gtcaccgctt
     9901 caaaggctgc tgctgcttga tagatggttt cttcagtgta tttcggacca attaattgaa
     9961 gccctactgg caagccatcg acgaatcctg ctggaatgga aattcctggc agaccagcca
    10021 agttaactgg aatggtcaat aagtcggcca aatacatggc aacaggatcg tggttgagtg
    10081 tgtctaagcc aaaagcaacc gtcggtgttg ttgggcctaa aataagatca taatccgcaa
    10141 agaccttgtc aaaatcttca ataatcaagg tacgcacttg accggccttt ttgaaataag
    10201 cgtcataata acctgatgac aaactaaagg tcccaagcat gatacgacgt ttaacctcat
    10261 caccaaagcc ttggctacga gtgttaacat agatttcatc caagttttta gcatcgtcag
    10321 cacggaaacc atagcgaatg ccatcaaaac gttgtaagtt tgaggacgct tctgacgaag
    10381 caataatgta atagacagcc acaccatatt tgctatgtgg aagactaact tcctcaacag
    10441 tagctcctaa agcttcaaat tgtttagctg ccgcaagaac cgtttcctta atctcaggat
    10501 caatcccttc gccaagatac tctttaggaa gggcaatctt catgccttta atgtcacgac
    10561 caatcttaga tgtgtagtct gcaatgcgga caggcgctga agtggcatcc ttgacatcgc
    10621 tacttgcaat cacatttaat aattgggcgt tttctttaac tgtcggagca aaaggtccaa
    10681 tctgatcaag tgaacttcca aaagcaatga gaccatagcg agaaacagtg ccataggttg
    10741 gtttcaaacc gacaacacca ttaaatgcag ctggctgccg aatagaacca ccagtgtctg
    10801 aaccaagaga taaacggact tgtcctgaag cgactgctgt tgccgaacca cctgatgagc
    10861 caccaggtac ttttgtgtgg tcccaagcat ttttcgtttt cttgaaataa gaggtttctg
    10921 ttgaaccacc catggcaaat tcgtccatgt tggtttttcc aatgacaatc atgtctttag
    10981 cgtaggcatt tgctactgat gtggcatcaa aaatcggctc gtagttatac aacatttttg
    11041 aagcggctgt ggttaggatg cccttggttg agatattatc cttaacagct agtggaatac
    11101 ccgacatgag gttatcagca tcaatacctt tggcatcaat tgccgcagct tgtttaagag
    11161 cagcttcttc tgaaacagtg ataaacgacc cgacagcctc ctcacgagac ttgatgtctt
    11221 caagtgtttt ttgagtcaat tcggttgcgg aaatctcctt agctacaagg aggtcatgca
    11281 actcttcaat ggttttatga ttaaaagaca ttaggcatct cctccgtcat ccaaaatagc
    11341 aggtaccttg ataaagtgat tttctttttc aggaacattt ttaaagagca aggcacggtc
    11401 tgttccttct tcagcgacat cttgacgcat cacatttttc ttatctgcca tggttgttgt
    11461 aatggcaaca ccttcggtat caacttcgtt cagcaattca accatgtcaa cgattttaga
    11521 tagggtcgtt gcaaaggttg ttgtctcact ttctgaaaat gataattttg atagcttcgc
    11581 gacatggcga acttcttctt cagaaatttt catgatttct cccatccaag atgacaagaa
    11641 gtattccagt tccaaagaac agctctgcta ttctctaaag gtagcggaaa aacttctcac
    11701 atcattttta catagtctca attattatat cacaattgtt cccatttacc ataggtgact
    11761 aatgcaattc tagaaagcca tacagcaaaa aacttcctga ctaaggtcag aaaaacttag
    11821 tcaggaagtg tgacaaacta aactctattt gtcttgggta agactgtggc ctgaagcttt
    11881 gatggcagcc tgagcagccg ccaagcgagt aagcggcact ctaaatggcg aacaagaaac
    11941 gtaggtcagg ccttggctgt ggcaaaaggc aatggaagca ggatcgccac catgttcccc
    12001 acaaatgcca agtttcaact taggcttaac cgaacggccg gcctaatgaa acggcggtac
    12061 taaagagacg gccaatccct ttctgatcaa aggtctggaa cggatccttg tcaaaaatac
    12121 ccctgtcaac gtattcttcc aagcattttc ctacaacatc acagaaaaag taacgttctt
    12181 tcaagtcggt aatatgttta cgaaggtaat aaaggggtgc tttattctaa tttaccaaat
    12241 cctcaacgat tgtgacaaca tgtgatgtga taagcctaag ataaaaatgg tacagactct
    12301 agcattcaag agtttgaagc tttatttata ttctaatgct gaaaagacta gagtttgtct
    12361 aatggctgtg agacgatcct ttaaaccatc aactcaaatc atttctcaaa cagctaaaat
    12421 cctgtcttct tcaaaaaaac atgtgttcta tctgacacag tccaaagttt agttgccttt
    12481 tagacagcac aagaatcatc aggactaatt ctgaccagca tgtcacatac ctcatctacc
    12541 taacccatac atagggtaaa accacataga gcaatgatta cggttaccaa actcttagtc
    12601 actgaatcaa tagtttggta ggctctttta gtaatcgtta ataagcttag caccaagaac
    12661 ctgctcaaag tgagccaaag accactcata agattctttt gtaaggctag cgacagccga
    12721 tttaacaatc tctaattgat aaccaagatg ataagcgtca atagcagtat gtaagacaca
    12781 gatatcagat aaaactcctg ttaacactaa ttgagtgata cctctttctc gtagtctaat
    12841 atctaaatca gtccccgaaa aagctgaata ataacgctta tcaatccaaa aaactctggg
    12901 atgttgcttc atataactgt aaacttcagc tagtggacca taaagatggc gtccagtagt
    12961 tcctttgata ttatgtgctg caaataactt actctcagga tgccaaggat cattttgatc
    13021 atgacaatca atagcaaaaa aaatatagtc tccttgatca aatgccgttt tagttacctc
    13081 tgctatttta gtggcaatag cctgagcaga tttacctgca cttaatttac catcatctgc
    13141 tacaaaatca tttgtgtaat caattgaaat caatgctctc atcactatct cctaaacagg
    13201 gaaacctctc cccttatctc ttatatatgg cattgtatca gtttttatta caggtatcaa
    13261 gacatctatt tgcatctacc attaacagca aaaaacatct cttgaaaaga gatgttgtct
    13321 ttttctattt aaaattcttt taatttagca aaaatacctt cgttgataac tttgaggtat
    13381 gtccctttca taccaagaga acgactttca ataatccctg cactttctag tttacgcaga
    13441 gcattgacaa taacagaacg ggtaatacca atacgatcag caataacaga agctgtcaat
    13501 cgtccttcat taccatctaa ctctccaaga attgctgcaa cagctttcat ttctgaataa
    13561 gaaagcgtat taattgccat gttgaccgct gtttgtttac ggatggtgtc ttctaagttt
    13621 tctgtctgaa gatttaatag ttgaatcccc acaacagtac ttgagatctc aaccaaaatc
    13681 aaatcatcat cactaaactc atcgtcatta cgccagataa tgagtgatcc aaggcgcatc
    13741 cctccaccat aaatcggcgc aatagtcgtt agacctcctg gataagtgtc tttagactca
    13801 acagggaata tagtcaattc attttcgaca gaaaggttag cttctgtatc gtaaactcgg
    13861 ctagcagcct ttacataagt atctggaaat tgtttagctt caaaaaattc ctcaacacga
    13921 tcagtgttag ttttatattt catggcataa ccaagcagtg tgccgccgcc attgataata
    13981 caagcattgc agtcaatgat atctgctagg cgagatgcca ttgtgttata cggtaattct
    14041 gtttctaggc tatctacgga acgctgcaaa atagatgtga ttttacgagt tttttctaat
    14101 aagttaggca ttttttcctt ttcctttgaa ttatttgcgc tttcattata acaaagctaa
    14161 ctcgcttttg caaggaatta tcagaaaatt ttctattctt actacaattc caactattga
    14221 tgccttatta tcacttcatt taaaggagat aagcacttgc ttgttagagc atagggtcac
    14281 attcttttaa gaaacgttat ttttattaat caaagatgag cattgcatta acgtctatac
    14341 tggtataaga cacgtgtaat cttttcttgg acatcttcta attcttctac acttggtagg
    14401 taaacaatac ggaagtgatc tggatctttc cagttaaaac cacgtccatg taccaacatc
    14461 actttttctt gctttaataa ctgtaagaca aattcttcat catcatcaat acgatacatc
    14521 tgacgatcaa ttttcggaaa taaataaaga cctgcttcag gtttaactgc cgataagcca
    14581 gggatagcat taatagcttc ataaataaaa ttacgttgct caaaaatacg ccctcctggc
    14641 agtaacagtg catcaactga ttgatggcct cctaaggaag tttgaacaac ttgttgggct
    14701 aaaacattgg agcataaccg catattggca agcatgttca acccttcaat gtaaccttta
    14761 acatggtctt taggacctga taagaccatc catccaacac gaaaaccagc aatacgatga
    14821 gatttggaaa ggccattcat agatacacaa aaaacatcag gtgccaaact tgcaatagca
    14881 atatgctctt taccatccat caccaaacga tcatagattt catcggcaaa aataatcagc
    14941 tggtgttctc ttgccaaagc aacaatgtct tctaaaatct ccttaggata aagagctcct
    15001 gttgggttat taggattgat gaccacaata gctttggtgc gactcgtgat ttttgatttg
    15061 atatcagcaa tatcaggata ccaaccagct tcttcattac aaagataatg gaccgctttt
    15121 cctccaccaa gactcacaca agccgtccat aaaggataat ccggcattgg tactaaaact
    15181 tcatcgccat tatctagcaa agcttgaagg gacatggata tcaactctga cacgccattt
    15241 cccaaataaa tatcttcaat gtcaacatct gggaagccct tgagttgaca atattgcatg
    15301 atagccttac gcgctgaaaa aatcccttta ctgtcagaat agccctcact tagcctcgcg
    15361 ttgacaatta aatcacgaat cacttcatct ggtgcttcaa aaccaaaggc agcaggattg
    15421 ccagtattta aacgcagaat tttttcacca ctcgcaatca tgcgattagc ttcatccaaa
    15481 acaggccctc gaatatcata ggcaacatgt tctaattttg atgatttttc aataattttc
    15541 atagtctcat acacctcgtt ttccatatta taaaccaaaa tacttaaaat agaaagaatt
    15601 tatccatcaa agcttaggac agctaatcgt tttaacacag gtcttatgag acgataatac
    15661 agtctttagt ttagcaaaaa caatggatcg ctttcaaaat gactcaaaaa agagtataat
    15721 gaaaaaaaga ataaaagcta ttcttgtcaa actgttaaag gaggacagtt ctatgtcact
    15781 aaaatataaa cgtatcctcg ttgctattga tggctcttat gagtctgaat tagctttcaa
    15841 taaaggcgtt aacgtagcac tacgtaacga cgctacctta ctactagtac acgttattga
    15901 cacccgtgcc ttgcaaagcg tagctacttt tgatacttac atctatgaaa aattagaaca
    15961 agaagctaag gacgttttag atgattttga aaaacaagca cagatagcag gaattacaaa
    16021 caccaaacaa attattgaat ttggcaatcc caaaaactta ctagctcacg acattccaga
    16081 tcgtgagaac gctgatttaa ttatggtagg agctactggt cttaatacct ttgaacgcct
    16141 ccttattggc tcatcttctg agtacattat gcgacacgct aagattgact tacttgtagt
    16201 ccgagatagc actaaaacac tctagtctat cacaattttc aaacgccaaa aaacaagcag
    16261 catctacaac gaaattgctt gttttttgtg ctgagctaag tctagtcaat atctttttat
    16321 ttcttgatat ttaaaagagt aaacctctat ttatcgaatc gttgtagtga tttttgtaat
    16381 tgtttgtcta actctttttt tatggctact tcaggtgcgt gacgctcttg ccaatagtca
    16441 ggtttttgaa ttttatgggt aacggggtca aagtgtgcct tgccatctgg aaaaatttta
    16501 gccatatttg cttcatgtac tgcttcaaat ataggttgtg gatctactcc catcagtaca
    16561 aaagaaccat aagtaaaata aagcaaatcc gctaaggcat caacttgccc aatcaggggc
    16621 gtttcaacat gttttttagc ttgtacctta ttagctgctt ggtcaacagc accatgcaaa
    16681 tcgaaaatag cctgtgtaaa ttgttgttgg tctcccttgc ttgctgcgta aagaaactca
    16741 acaatttctt ctactttgaa gcctgaccta taaccagctt ctgaaatggt ataactacgc
    16801 ggtgtttcaa tagtatctga gtccatcaga cgatgaaaat ctttgacttt attaaaattt
    16861 tcatcacggc ttttaaagct cttctctata tcaaaatgga tgagaccgta gtgagctaag
    16921 gctttggaaa tcccatcatt attattagag tcggtagtga aatgggctcc atcttttacc
    16981 acagtttcag catttcccat agcaataccg ataccaactc ctgataacat ttcaaggtca
    17041 ttatctgaat ctccaaaagc catcacttca gacaagtcaa atccaaacat ctctcccaaa
    17101 cgttcaattc cttttatttt tgattgatca acagagatca gatccaagga ataaggacta
    17161 cttcttgtaa tttttatatg agggaacttc tcttgtattt tcttagtttc agcttgtgac
    17221 gctaccagaa caacctgata aataggctcc cgcataatag ttactaaatt tctaaaactc
    17281 tgaggcttga ttcggcgaat caaatgctta aaactaccct caactgttct tgcccaactt
    17341 ttgggcacaa aactactaat gacttgtcca aatgggctag tccccatatc aataatacgc
    17401 gatcctgcta atccagaagc tgttccaaga gaaatttctc gctttttttc gttggcatag
    17461 cgaatcactt tgtaaatcat cgactttggc aattgattct gatataagac cttatctctt
    17521 gttaagatat attgcccatt gtaagtaacg gcaaaatcta atccaaaatt ttctagaaaa
    17581 ggttgtacaa atcccggtcc acgaccagtt gctaagccga ccataatgcc ttgttttttg
    17641 agttgttgga tggctttttg cgttgttttt tggatatttt tacgatcatt gagaagggtc
    17701 ccatcaatat caaaaaagac tgcttttact gtcaaaacct tcttttgcct cctgtgttta
    17761 tgatagaata gattatatca taaataaaag gaagtttgac aatcaaatga aaaaaatcct
    17821 agtcttacac actggtggaa ccatttctat gcaagctgat aactctggta gggttgttcc
    17881 aaatcaagat aatcctatga cgaaaataca tgctgcagtc caagatattc agcttaccgt
    17941 tagtgatttt ctaaatcttc cgagtcctca catcacgcct caccacatgt tatctattta
    18001 tcatcatatt caagaaagaa ctgatgtctt tgatggtata gtcatcacac atggaacaga
    18061 taccttagaa gaaacagctt attttttaga taccatggca ttgcctacta atatacctgt
    18121 tgtcctaaca ggtgctatga ggtcttctaa tgaagttggt agtgatggca tatataatta
    18181 ccttaccgca cttagagttg caagtagtga taaagctaaa gaaaaagggg tacttgttgt
    18241 tatgaacgat gaaatccatg ctgctaaata tgttactaaa acacatacaa ccaatatttc
    18301 aactttccaa acacccacac acggaccact tggtatcatc atgaaaaatg gcctactttt
    18361 tttcaaaact gctgaacctc gtattcgttt tgacttgaga tgcatttctg gaaccatccc
    18421 cattatcaaa gcatacgcag gtatgggcga tggtagtatc ttgagtctat taactccagg
    18481 tagcattcaa ggtctagtta tcgaagctct aggagcagga aatgttccac cattagcagt
    18541 tggggagatc gagcacttaa ttactttagg tattcctgtc gttcttgttt cccgttgttt
    18601 caatggtatg gcagaacctg tctatgctta tgaaggtggt ggtgcgatgc tacaagaagc
    18661 aggtgttatg tttgtaaaag aattgaatgc tccaaaggcc cgtctaaagt tattaattgc
    18721 tctcaacgcc ggacttactg gtcaagagct aaaagattat attgaaggat aatctagctt
    18781 cctgtagaaa tgtcccacta aaacatatta gtgggacatt tctagtactt taagctttaa
    18841 attaggacat ctcctaaatg tctataataa acaactatgt ttcaaactat gagattgtgc
    18901 actcataatc atttctataa cagtttgaga ccagtattcc aataaatcca actcttagca
    18961 atcagatacc gggtaaacaa gtctttattt tgtctcatta agtataagct tacttgaata
    19021 ataccaaaac tacttgctca tcctttttta gtaatgatga agacgacctt tttctaagaa
    19081 cttctttgtc ttttaaaata aacaaagcac atattaccct ttattttagt tctcgtttgg
    19141 aaaaaagaaa gacattgcct aaaagataac aaagagttaa accaaaggcc actaatatgc
    19201 tagtactaga aagatgttta tcaccaagag acaaggatag gtgtttaaac atcaaaactg
    19261 ggctaatatc taacttaaaa aaatggtttg ctaaagtagc agatacttta tcaaaaagaa
    19321 ggacataaag tatataagct gtgattgact gataagtttt acgaagtata ttaaacaaaa
    19381 attgacacaa agcaataata gcaatataaa aaggcagttg tttaatcatc cgatctatga
    19441 ctattccata gtatgcttga ccagtaaata aatgaaatgc taaagcacta agaagataag
    19501 ttaaactaat aaataaaaga gataagccaa aactaactag cagtttatag attataatta
    19561 aagtacgatt ttgttgtttg accacaacat tattaatcgt acggtagtta tagtcatcac
    19621 taaaaaagat acgggctggt gttaaaaaga aaagcgtcac aaaatcagta ataatattaa
    19681 tgatttgtat catggtgact tggttaggtg tcttgcgaaa actataggct acaccaacag
    19741 caatgacaac aagtagcata caagaaaata gagctaaacg ctcttgacgt atgcgaaata
    19801 aatctgcttt aagataggaa atcataaaaa cctctttcta aattaatggt gacgctgcat
    19861 ctttacctaa caaatcgagg taataatgtt caaagctcac ttgatgatga taaatgtcaa
    19921 aaatctcaac aggcagatct agcacaattt tgataagctc cattactgat aaagttggtt
    19981 ttattagtag catctgacca tcagtctcaa ctctaagccc caattctaaa agtctatttt
    20041 ttaccagctg atcatcgctt gtatgaaggg caatttgtac ttttacttga gctttaagct
    20101 ctgctttgtc catactctta ataactttgc ctttatgcat aatcacgtaa cgatcaacaa
    20161 ctaagtctaa ttctgataaa atatgactgg aaatcaaaat cgtaatacca tagcaatctc
    20221 ttaggttcaa aataatctga cgcatttctt tgattcctgc tggatcaaga ccattaatgg
    20281 gttcatctaa gattaaaaat tctggaaaat ctagtatcga aagaccaata gccaaacgtt
    20341 ggcgcatccc caaagaataa tctttcacct ttttcttttt gctcactttt tctaacccaa
    20401 taagttccaa aacctcatgt atgcgctctt ttgcatcagt taatctcaat tgcaacgctg
    20461 catagtaaag attatcatga gctgtcatat tgggataaag tgtcggtgac tcaataattg
    20521 atcccattcg atagggttgt aactgtttga tttgccctga atcttttcca attaggccag
    20581 ataaaatacg cattaacgtt gtctttccag caccattttg acccacaagg ccgcaaattt
    20641 ctcctttgta aatatcaaaa gagatatcac ttaaaacctg atttttacca taggatttat
    20701 ttacttgctc aatacttagt aacattgatt gctgtgtcat tttccgctcc ttttttgtac
    20761 ttatcgatta atacaatatc ttacacataa taacatctgt aaacggttgt gacaagttct
    20821 tctttttatt aaaaaaatat aaataagaat tttaataata tgattatttt agcaataaaa
    20881 aagttatact gaaaatgggt taatactgct tcaaatagca gtctcatcgt aaagcataag
    20941 taaaaaaggt cagaaatagt gacctcttta tactt
//
LOCUS       DAUELZ010000019        20963 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.019, whole genome shotgun sequence.
ACCESSION   DAUELZ010000019 DAUELZ010000000
VERSION     DAUELZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20963
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.019"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            146..868
                     /locus_tag="VHI09_000571"
     CDS             146..868
                     /locus_tag="VHI09_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ6120471.1"
                     /translation="MPKEQPLYLQIVDALEVKIRKSMTANDKLLSERELSDLYGVSRI
                     TIRLALKELELRGLIYKKQGKGTYVSGIKEPATDLSSTYSFTEQMKKMGKMPKTEILS
                     FEQYQVTPYLSGLLELDPDTEVFELERLRIADDMPLMFERSYIPAQPFQGLSIADLKR
                     KALYDIFAKDYQETIRLAEEEFYASIALDYEADLLGIKKGDPVLHIIRKTYNDKNILI
                     EFTFSIARADQFRYRVQHHPNG"
     gene            888..2087
                     /locus_tag="VHI09_000572"
     CDS             888..2087
                     /locus_tag="VHI09_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEQ6120472.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFDETRDSLDS
                     FLKRVCKSANGNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQCYLFESVASTDLVAAP
                     DYYLAEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATVYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKTLALLQGGDINFSEDQFRL
                     DTRHLLPDAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGMVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(2185..2445)
                     /locus_tag="VHI09_000573"
     CDS             complement(2185..2445)
                     /locus_tag="VHI09_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEQ6120473.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(2560..3501)
                     /locus_tag="VHI09_000574"
     CDS             complement(2560..3501)
                     /locus_tag="VHI09_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEQ6120474.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLAWMSQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            3895..4344
                     /locus_tag="VHI09_000575"
     CDS             3895..4344
                     /locus_tag="VHI09_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEQ6120475.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSEQFALTGAIKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            4520..4804
                     /locus_tag="VHI09_000576"
     CDS             4520..4804
                     /locus_tag="VHI09_000576"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEQ6120476.1"
                     /translation="MRLEEIRQEINGIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QVRENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQHLTGGDIND"
     gene            4797..6059
                     /locus_tag="VHI09_000577"
     CDS             4797..6059
                     /locus_tag="VHI09_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEQ6120477.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGVAVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCQRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFACLLGWFKPYLSQKLPNPYLRIAFIGVLLSICLMIGHV
                     GRYSGLGTHLLAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYVTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMIS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            6174..6521
                     /gene="rplS"
                     /locus_tag="VHI09_000578"
     CDS             6174..6521
                     /gene="rplS"
                     /locus_tag="VHI09_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEQ6120478.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            6768..6839
                     /locus_tag="VHI09_000579"
     tRNA            6768..6839
                     /locus_tag="VHI09_000579"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6801..6803,aa:Arg,seq:cct)
     gene            7549..8118
                     /locus_tag="VHI09_000580"
     CDS             7549..8118
                     /locus_tag="VHI09_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEQ6120479.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVATFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            8119..10071
                     /gene="gyrB"
                     /locus_tag="VHI09_000581"
     CDS             8119..10071
                     /gene="gyrB"
                     /locus_tag="VHI09_000581"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEQ6120480.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLDAVRKRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYNVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRDFIEENAVYSTLDI"
     gene            10439..12163
                     /gene="ezrA"
                     /locus_tag="VHI09_000582"
     CDS             10439..12163
                     /gene="ezrA"
                     /locus_tag="VHI09_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEQ6120481.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKHNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(12295..12753)
                     /locus_tag="VHI09_000583"
     CDS             complement(12295..12753)
                     /locus_tag="VHI09_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEQ6120482.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQTPEKTKSFWKRFLKK"
     gene            12980..14287
                     /gene="eno"
                     /locus_tag="VHI09_000584"
     CDS             12980..14287
                     /gene="eno"
                     /locus_tag="VHI09_000584"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEQ6120483.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(14762..15792)
                     /locus_tag="VHI09_000585"
                     /pseudo
     CDS             complement(14762..15792)
                     /locus_tag="VHI09_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677767.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16059..17600)
                     /locus_tag="VHI09_000586"
                     /pseudo
     CDS             complement(16059..17600)
                     /locus_tag="VHI09_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284650.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            18073..>20963
                     /locus_tag="VHI09_000587"
     CDS             18073..>20963
                     /locus_tag="VHI09_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ6120484.1"
                     /translation="MFLKHQDVKQKNWRMRKVKKLFVSSCMLLTVGLGVAVPTGFSQS
                     NGVMVVKAAEVPAAEGVFKIETDNLQNWEAETNTQGYKGTFSKSPDSQGFYKVQTNSG
                     AGIGVQVAPKGNGRMDIKIGNRVEIPKGYYVGGIRTKTPVLEKDNTYRLSFTTSKTSG
                     TDVEFKTRIKDVEANGDLGQEHTIKFDGNTVSTGNPWIKGLTNKKHTVDFIGKGKALS
                     MAFRFSPFKTTNITYTFTDMNISNVTPASVPETPSSVYEEATKISGKAVASKDNFNKR
                     TNFLGDVVNVYKLDQLVGTAQVKSGNTFEVVLNLKEPLKQGEKLEVEIQNPKSALLSA
                     RVPINVIAKPYDKAQHLKDSKASLDEAYRQTVENITQDNWLKSSEKQKQTNAAKLAYD
                     QGIIELGRSNDKAAINSVVTKYTQKKTGLITKEHILGEKDKVITAAKNAIEEKATAEK
                     TKIDSDNSLTTPEKEAQLAQLSTAKEAALTAITESPDADTVGTKQTEGEASVSGIHQG
                     KDLEAIKTAAKNAIEEKATAEKTKINNDITLTAKDKEQQLKEVETALTKAKDNVKAAK
                     TADAINDARDKGVATIDAVHKAGQDLGARKSGQVAKLEEAAKATKDKISADPTLTSKE
                     KEEQSKAVDAELKKAIEAVNAADTADKVDDALGKGVTDIKNQHKSGKGLDVRKDEHKK
                     ALEAVAKRVTAEIEADPTLTPEVREQQKAEVQKELELATDKIAEAKDADEADKAYGDG
                     VTAIENAHVIGKGIEARKDLAKKDLAEAVAKTKALIIEDKTLTDDQRKEQLSGVDTEY
                     AKGIENIDAAKDAAGVDKAYSDGVRDILAQYKEGQNLDDRRNAAKEFLLKEADKVTKL
                     INDDPTLTHDQKVDQINKVEQAKLDAIKSVDDAQTADAINDALGKGIENINNQYQHGD
                     GVDVRKATAKGDLEKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKNTA"
BASE COUNT         6613 a         3623 c         4399 g         6328 t
ORIGIN      
        1 caaacctaag aaagattagg tttgcttttt gttttctttc ttttattttt gatcaaaaaa
       61 ttttaatatt tctaaatatt tgcttgacta agaattatta ttataatata ctttagatgg
      121 ttataacaag ttagaggtga ataagatgcc aaaagaacaa ccactctatt tacagattgt
      181 tgatgcccta gaagttaaaa tcagaaagag catgacagcg aacgataagt tactgtcaga
      241 acgagaacta agcgaccttt acggtgttag ccgcatcacg attagactag ctcttaaaga
      301 attagaacta cggggcttaa tttataaaaa gcagggcaag ggaacctatg tttctggtat
      361 caaggaaccc gccactgatt tatcaagtac atatagcttt acagagcaga tgaaaaaaat
      421 gggaaaaatg cctaaaacag aaattctttc ctttgaacag tatcaggtaa caccttactt
      481 gtctggtctc ttagagcttg atcccgatac agaagttttt gaattagaac gcctaaggat
      541 tgcagatgat atgcccttga tgtttgaacg atcctatatt cctgctcagc cttttcaggg
      601 gttaagcatt gcggacttga agcgtaaagc tttgtacgat atctttgcaa aggactatca
      661 agagacaatc cgtcttgcag aagaagagtt ttatgccagc attgccttgg actatgaagc
      721 agaccttttg ggaatcaaaa aaggagatcc tgtcttacat attattcgta aaacttataa
      781 cgataaaaat attttgatcg aatttacttt cagtattgca agagcagacc agtttaggta
      841 tagggtacaa catcacccca atggttaaag aaatgggagg ttaatacatg tttacaaaaa
      901 cgcaggaagc tttggaggct cttggtgcag ctatcactac caaggaaatt aaacaagaac
      961 cccgtttatg gcaagaaacc atgacgtttt ttgacgaaac aagagattct ctggattctt
     1021 ttttaaagag agtttgtaaa agcgctaacg gaaataacgt tcatgtcatc tttacaggtg
     1081 cgggtacatc tgagtacatc ggtaatacca tttgccctta cttgaagaag gtggggaatc
     1141 ggcagtgcta tctctttgag agtgttgctt caaccgattt ggttgcggcg ccagattatt
     1201 atcttgctga ggaagagacg gtactattag tctcctttgc taggagtggt aatagtccag
     1261 aaagtgtagc ggctgttaac ctggtcaatc aactagtgcc aaatagttat catttaacca
     1321 ttacgtgtgc caaagatgga gaactggcga aaaaagcaca gcaagatgag cgttcttacc
     1381 tttatttgat gcctgaagac gctaatgatg ctggttttgc catgactggt agttttacct
     1441 gtatgatgct agcggctctt ttgatttttg atgatgctaa cagtatagct cagaaagaag
     1501 cttatgtgac tgatatgatt cttgctgggc aagctgttat tgaccaagaa agacggctac
     1561 aagagctagc tgatttagga tttgaacgtt tagtttatct aggttctgct ggtttagcca
     1621 agttaaccca ggaggctcaa ctaaaaatgc tagaattgac tgctggtcag gttgcaacag
     1681 tttatgagtc ttcgatggga tttaggcatg gtccgaaatc ttttattaat gaccgtacct
     1741 tagtcattgg atttgtcaat aatgatgctt acgtcagaca gtacgatttg gacatgttag
     1801 aggaaatcca agcagatggc attgccctta aaaccttggc tctattgcaa ggaggggata
     1861 tcaacttttc agaggaccaa ttccgactag acacccgtca cttattgcca gacgcttact
     1921 tagcttttcc catgatttta gtcgcgcaga ccttggccct cttaacggcc gttacattag
     1981 gaaattcccc tgacacccca tctgcgacag gaatggttaa tcgtgtggtc aaaggagtaa
     2041 cgattcaccc ttacccgact agtagtcgtc aatgcaacaa agcttaaagc ttttgtgtag
     2101 aagccgtaaa acgaaatgat tttgtgctag taggccaaaa gaaaagaccc tcctgataca
     2161 ggaaggtttt ttgtattatc ttgcttattt tgctgcgttt gcgtctttga gaccgtattt
     2221 tttgttgaag cggtccacac gtccatctgc ttgagtgaac ttttgacgtc ctgtgtagaa
     2281 tgggtgtgaa tctgatgaaa tttccacacg gataagtggg taagtttcac cttcaaactc
     2341 aacagtttct ttagaagctt ttgttgagcc gctaaggaac tggtaacctg tagttgtatc
     2401 taggaaaaca actggacgat aatctggatg aatgtctttt ctcatcttaa aaaattcctt
     2461 tctgccatga tacttgatgc atcatagtta ttaactattc tagtgtaaca aaacttgaat
     2521 gacttgacaa ggcttttctt tccttatttt ttaaaaatcc taaatctctt ggcaaacagc
     2581 gatgagttct cggaaaatag cttgattttc ttctaaatta gctgagttgg ctccgctagc
     2641 aaggggatgc ccccctccac cgtgacgctt agcaatgcca ttaataatct tttctttact
     2701 gcgcatacgc acacggtagt tgccgtcagt taactcaaca aaaatagccc aagcttgaac
     2761 gttatcaatt ttaccaggag cacagacaat ggcagagctt tctgctaggg tcacgtcaaa
     2821 atgttttaag gtttcttggc tgacaaggac ataagcagcc ccactctcat caattgttaa
     2881 atgctcaaag acgtagcttt gcagctttgc tattttcaaa ggaaacgaat ccatttgcct
     2941 tgaaatcgca gcaaagtcga actcgaaatg tctgagttgg ctagcaatgg aaagggtttt
     3001 actagtggtt gaggcataaa gaaatcgccc tgtatcacca acgataccag tgtataagag
     3061 cttagcagcc ttgtcagaga gagtaagatt ctgactaaag gcaaagtctg caatgatttc
     3121 acttgcgcta gaagcgcttg tgtccacata atagaagtca ccatacacat catcgttggg
     3181 atggtgatca atcttaatta agcactgccc cagtgtgtag cgctcatcat caatccttgg
     3241 tcgattcgct gtatctgtaa tgatgaccaa agcctcttta tagtctttat cagtcacctg
     3301 atccatttgg ctcatccaag ctaaactagg ctcatcaaaa ccagtcatta aaaccttttt
     3361 atctgggaaa ttttgtgcaa taatttcttt caagccggcc tgactaccaa gagcatcagg
     3421 gtcaggattt tgatggcgat ggataataat agtttggtga gcttttattt tatctaaaat
     3481 tgtttcaaaa gttgttatca tgctttaatc tttctttttc aaaaagagga aaataaccgt
     3541 ttaacatagt cttcaaaaaa cggtttgtgt cataaggagg tgtgcgttat gttaagtggc
     3601 taatgctaga attttttaag attgtcaaag accataaatt gacattttat ctgaaaatca
     3661 tatcctattt tattatagca taaaagtaag ctctagataa tggtaaatta atctaggaaa
     3721 aaaagaggtt agttatcagt tatgatacta tcaaagctag tcttgcaaat gcaagtgaaa
     3781 aaatagtttt taagtgtttt aagtaatcat atcaaccttc tcgttaaaca attagtgctt
     3841 ttcctaaaaa agtgtgctaa aataaacata gtttagtaat tttgggagga atttatggca
     3901 ttagcaaaaa tcgtttatgc cagcatgacg ggaaatactg aagaaatcgc tgatatcgtt
     3961 gcaaataaat tgcaagaatt gggccatgat gttgacattg atgagtgcac aacagttgac
     4021 gcttcagagt ttgagaatgc tgatatcgct gtcgttgcga cttatactta tggtgatggt
     4081 gacttaccag atgaaatcgt tgatttttac gaagatttac aagatttaga cttagaaggt
     4141 aaaatctatg gcgttgtcgg ctctggtgac actttttatg actatttttg caaatctgtt
     4201 gatgactttt cagagcaatt tgcgcttact ggtgccatca aaggtgcaga gcctgtcaaa
     4261 gtagacttgg cagcagaaga tgaagacatc gatcgtttag aggcctttgc agagcaactc
     4321 tcacaggctg ttaataatct ataaaactat ttagtaaaaa tgtcagggac ttccttggca
     4381 tttttagatt tttaacctaa aataaaggga atgtcacttc agggtaggaa ttgaaaaaaa
     4441 caattagctt caccttgctt ctgatcctct tattaactat aataaaaaga taatctaata
     4501 catctagaaa aggaaagtta tgcgtttaga agagatacga caagaaatca atggtattga
     4561 tcaccacttg gttgccttat tagaaaagcg gatggcactt gtagagcaag taacagctta
     4621 taaactagcc aatcacttgc ctgtcttaga tcaggtgcgg gaaaatcaga tcctagatag
     4681 ggtttcttat ctcgttaaag accaagcatt tgagccagct atccatgaga cgtttaaaac
     4741 cattatgtct ctttcacggc agtatcaaac gcaacactta acaggtggtg acattaatga
     4801 ttaaatcaat tgcaacactg cctaaaccct ccttaaaaga agcgttagga ctaataggtt
     4861 tggcgcttcc cataggggtc gcggttggag tggttgatgt gatttttggt aaagggcttt
     4921 tgtttctttc cgagtatcgc gatcaccacc ttttcttgct tcttcctttt cttgccctag
     4981 caggtcttgt tatcgttttt ctttacgata agttaggcaa agaggttcgt caagggatgg
     5041 gattggtttt tcaggtaggg catggtcaaa aagatcagat tcctcctatt ctcattcctc
     5101 ttattttgtt ctcgacttgg gtaacccact tatttggagc gagtgcgggt cgagaagggg
     5161 tagccgttca aataggagct accatctctc attattgtca gagatttgtg actagtcaag
     5221 aagccgcacg acacctttta attatgggga tggcagccgg ttttgcagga ctttttcaaa
     5281 cgccgattgc tgctgtcgta tttgccctcg aggttttatt ggtaggaacg cttcgctact
     5341 gcgctttatt accttcctta gttgctgctt gcgtggcttc ttggacctct cattctttgg
     5401 gattagagaa atttacgatt gttcttgaag aaacattgac cataactccc ttaaccttag
     5461 ttaaattaat tgggttaggt cttatttttg gtctggtggg aaatagtttt gcctgtttac
     5521 ttggctggtt taaaccttac ctgagtcaaa agctaccaaa tccttatctc aggattgctt
     5581 tcattggtgt tttgttatct atctgtttga tgattggtca cgttggaaga tatagtggtc
     5641 tagggactca tttgcttgcg gcagcttttt ctggccaaac tatcctaact tacgactggc
     5701 ttttaaaaat gatcgtgacg gtgattagct tatctgcagg ctttcaaggt ggtgaagtga
     5761 cgcctctatt tgctattgga gcttctttag gtatcgtttt ggccccttac ttaggtcttc
     5821 ctgtactgtt ggttgctgca cttggttatg tcacggtatt tggaagtgca acaaatacct
     5881 tttgggcccc tatctttatt gggatagagg tctttggtcc agagaatgcc ctagcatatt
     5941 ttgtcacctc ggctgcagcc tatatgatta gtcacagaca ttccatttac agttatcaaa
     6001 aagtagtcat gatcaattta aaagacatga cggtaaatca gatggattta aaaaaatagc
     6061 tttacaataa agaagctttt tgttagaata aatctcgtgt gtaatggaca cacgaaaatt
     6121 acggctaatc cgctaagaca agtacttaag attagtaaga taggagaata aaaatgaatc
     6181 cattaatcca aagtttgaca gaaggtcaac ttcgctctga tatccctaac ttccgtcctg
     6241 gtgacactgt tcgcgttcac gcaaaagttg tcgaaggaac tcgcgaacgt atccagatct
     6301 ttgaaggtgt tgttatttca cgtaaaggtc aagggatctc agaaatgtac actgttcgta
     6361 aaatctcagg tggtatcggg gtagagcgta cattcccaat ccacacacca cgtgttgaca
     6421 aaatcgaagt tatccgccac ggtaaagtcc gtcgtgcgaa actttactac ttgcgtgcat
     6481 tacaaggtaa agctgctcgt atcaaagaaa ttcgtcgtta gtttgaaccg aaacgtaacg
     6541 gaatagaata aaaagactag agtctctagt ctttttatgt cgtcgtgaca atgtgataat
     6601 cggtgattaa cgctatgctc gtaggacact ttgaaagttc cttagcagtt ataattagta
     6661 tattgtaata acaaaatgaa gcaaaataaa ctttcagtta tctgatattt catcttgcat
     6721 taccttattt tctaggttaa aatagaagac aaggtgaata taagatagac cccttagttc
     6781 aatggatata ataactccct cctaaggagt agttgctggt tcgattccgg caggggtcat
     6841 tattgtcact gaaaaagcct ttacatcaaa ggcttttttc tatgtactca attaatgtta
     6901 ggagcacaaa agaggcagtt cgattattaa ccaattcctc cctatttaca gttgtatggc
     6961 tataaatgga caatcttaag ttagggtctg agtgactaaa cctgtcccta accgcttttg
     7021 gtggtaattc tttttcagct aaaagcgaaa tgttcgagtg tttgaataag tgagtatggt
     7081 aatcaccata aatttttagg cgcttattga tgtgtgcatt taaaattggt aaaccttttg
     7141 aatatggaaa gctgttagca ctctgcttgt gattaatcta tcgtatgtta aaagtgacat
     7201 ttaaattaaa tcatttttta tacatatatt tcaacaggta tttcaacagg atttatcata
     7261 ttggaaaata gcggtgattc tattggtatt ttactattga caggaatttc cctgcctttt
     7321 gtctcctaaa gaagatcagc tttgatctct aacttgatta gagtgggctt ggtactgttt
     7381 atgaattatc agtacatact ttctggagat caaaaaggaa aggaacagct aaagaggtgt
     7441 atgtgttacc attagaggaa tagaacatcg caagactcgt ttattcagtc attacgatac
     7501 ctgttttatt taggaaataa ctataatatt aagtgaattt gtgatataat gaaaggtatg
     7561 aattatcaag attatatatg ggatttggga ggtaccttac tcgataatta cgagctttca
     7621 acccaagctt ttgtccaaac gctagctttt ttcagccttc caggagatca cgatgctgtt
     7681 taccaaaaat taaaagaatc aaccgctatc gctgtggcaa cgtttgctcc taatgagcca
     7741 gaatttttgc atgtgtatag gctaagagaa gcagacaaat tggcacaacc catttggtgt
     7801 ttgggagcta aagaaatttt ggaaaagata gcgacttcag gttcgcgaaa ttttttaatt
     7861 tctcatcgag attgtcaggt gaaccaactt ttagagcaag ctgggttgct aatctatttt
     7921 acagaagtcg tgacagcttc aaatggcttt gctcggaaac caaatcctga aagtttgttt
     7981 tatttaaaag aaaagtatga tattaatagt ggattagtaa ttggagatcg gctaattgat
     8041 aaacaagcag gacaagcagc gggttttaat accttgcttg ttgacggtag aaaaaaccta
     8101 ttggagatag taacgtagat gattgaagaa aataaacatt ttgaaaaaaa aatgcaagaa
     8161 tacgatgcca gtcaaattca ggttctagaa gggttagatg ctgttcgcaa gcgtccaggg
     8221 atgtatattg gctcgacagc taaagagggt ttgcatcatt tagtctggga aattgttgac
     8281 aactcaattg acgaagcatt agcaggtttt gcctctcata ttaaagtctt tattgaagca
     8341 gataattcca ttacagtagt agatgatggc cgtggaattc cagttgacat ccaagccaag
     8401 acaggacgtc ccgccgttga aacagttttt acagtcttac acgcaggtgg taaatttggc
     8461 ggaggcggct ataaggtttc tggaggattg catggtgtag ggtcatctgt tgttaatgct
     8521 ttatcaacac aattagatgt gcgtgtttat aaaaacggcc aaattcatta ccaagaattt
     8581 aaacgcgggg cagttgtagc agatcttgag gtcattggaa ccactgatgt gactggaaca
     8641 acggtacact ttacacccga tccagaaatt tttaccgaaa cgactcagtt tgattacaat
     8701 gttttagcaa aacgtattca agagttagcc tttttgaatc gtggtttaaa aatttccatt
     8761 acagataagc gctcaggtat ggaacaagaa gaacatttcc attatgaagg tggaattggt
     8821 tcttatgttg aatttttaaa tgataaaaaa gatgttatct ttgaaacgcc catctataca
     8881 gatggtgaat tagaaggtat tgcagttgaa gtagccatgc aatacacgac tagctatcaa
     8941 gaaacagtca tgagttttgc taataatatt catactcatg aaggtggaac gcatgaacaa
     9001 ggctttagag cggctcttac tcgggtcatc aatgactacg ctaagaaaaa taaaattctt
     9061 aaagaaaatg aggacaattt gacaggagaa gatgttcgtg aaggtttgac ggcggtaatt
     9121 tctgttaagc atccaaatcc tcaatttgaa ggtcaaacca aaacaaaatt gggcaactca
     9181 gaagtggtta agatcactaa tcgtctcttt agtgaggcct ttcaacgttt tcttttggaa
     9241 aacccacaag ttgctcgtaa gattgtggaa aaagggattt tggcttctaa agctagaatt
     9301 gcagctaagc gagcccgcga agtcacccgc aaaaaatcag gcttagaaat ttcaaactta
     9361 cctggaaaat tagcagactg ttcgtcaaat gatgctaacc aaaacgaact tttcatcgtc
     9421 gaaggagatt cagcgggtgg gtcggccaaa tcaggtcgta accgagagtt tcaagctatc
     9481 ttgcctattc gcggtaaaat tttgaacgtg gaaaaagcaa ctatggataa gattcttgcc
     9541 aacgaagaaa ttagaagtct ctttaccgct atgggtacag gttttggtgc agactttgac
     9601 gtgtcaaaag ctcgctacca aaagctggtt atcatgaccg atgccgatgt ggatggcgct
     9661 catattagaa ccttgctttt aactttgatt taccgcttta tgaggcctgt tctagaagct
     9721 ggctatgttt acatcgccca gccacctatt tatggtgtta aggtcggtag tgagattaaa
     9781 gagtatattc agccaggtat tgatcaagaa gaccaattaa aaacagctct tgaaaaatat
     9841 agtattggtc gttcaaaacc aactgttcaa cgttataaag gtcttgggga aatggatgac
     9901 catcaacttt gggaaactac tatggatcct gaaaatcgtt tgatggcgcg tgtgacagtt
     9961 gatgatgccg cagaagcaga taaagtattt gatatgttaa tgggagatcg tgttgaacca
    10021 agacgtgatt tcattgagga aaatgcggtt tatagtacac tggatattta gtcacctttt
    10081 tagtttcaca aggaatctgc taccaaagag gacgaattga ctaaaaatca tgatgcatga
    10141 tgccaacagc tttgttagct agtacgtctt catcttgagg gacagcgtta atgcataggt
    10201 attgagatcg tcccttaata cctatgttag tccttattaa gaaagccttg tgagtcgctg
    10261 gtacgagatt gttgaattag tcaaatatat tttgaaatgc attttaaagt atgttataat
    10321 cgtaatgtta tagtgtattt taaataaaat tttaataaaa ggcgattgag gaatttcagt
    10381 ttttagttat tactggaaca atgttgataa atcgtgtcta aataataagg agattcagat
    10441 gtcaagcgga attatcttgc tgattgtggc tatagtccta ctagtgatta tcgcctatct
    10501 agtgggtgtc atcatacgaa agcgtaatga ttccctaatt accagtctag aagaacgaaa
    10561 acaggccttg tttgctttgc ctgttaatga tgaaattgaa gaagtaaagt cacttcattt
    10621 gataggacag agccaaacct cttttcggga atggaatcag aaatgggtag acttaactgt
    10681 aaattcgttt gcagatattg aaaatcatat ttttgaagct gaaaatttga atgatacgtt
    10741 taattttatt cgcgccaagc atgagattaa tagcgttgaa agtcagctta atcttgttga
    10801 agaagatatc gcttctattc gtgaagcact taatatctta aaagagcaag aagaaaaaaa
    10861 tagtgctcgt gtcacccatg ctcttgattt atatgaaaag ctccaagctt ctatttctga
    10921 gaatgaagat aattttggtt caactatgcc tgaaattgac aaacagatga aaaacattga
    10981 aactgaattt tcacagtttg tcgcactcaa ctcatcaggt gatcctgtag aagcttctga
    11041 agttctggat agagcagaag agcatactat tgctctaggg cagattacag agcagattcc
    11101 agctattgtc gccaagttgg aagatgattt tccagatcag ttagatgatt tggaaacagg
    11161 atatcgccgt ttacttgaag aaaactatca ttttccagaa aaaaacattg aggcacgttt
    11221 tcaggagatt cgtgagtcta ttcgtgccaa ttcatcagag ttagttacac ttgacttgga
    11281 tcgtgctaga gaagaaaaca cgcatattca agaacgcatt gattctcttt atgaagtctt
    11341 tgagcgcgaa attgccgctt ataaggtagc tgctaaaaat agtaagatgc tacctcgtta
    11401 cctagcacat gtgaaacata ataatgaaca actcaaagat gaaattgcac gtttatcacg
    11461 taaatatatt ctaagtgaaa cggaaagtct gactgttaag gcatttgaga aggatattaa
    11521 agagatcgaa gacagtacac ttgctgttgc agaacaattt ggtttacaag aaaaaccatt
    11581 ttcagaactg caagtgactt ttgaacgcag tataaagacg cttactaatg tcgagtcagg
    11641 tcaaatggat gtttttgcag cagttaaaga tattgaaaaa attgaatcac aggcacgcca
    11701 taaccttgat gtttatgtta ctcagcttca tatgattaaa cgttatatgg aaaaacgtca
    11761 cttgcctgga attccacaag actttcttag tgctttcttt acaacgagtt cacagttgga
    11821 agctttgatg gatgagctta gtagaggacg gatcaatatt gaggcagtgt cacgtttgtc
    11881 ggaagttgcg acggttgcta ttgctaatct agaagattta acctatcaag tggttcaaaa
    11941 tgctaccttg actgaacaat tactacaata ttctaatcgc tatcgttctt ttgaagcagg
    12001 agttcaaagt agttttgaac atgctttgag acttttcgaa gtagaaaatg attatcaggc
    12061 ttcttttgat gaaatttctt atgctcttga aacagttgaa cccggggtaa cagatcggtt
    12121 tgttaactct tatgaaaaaa cacgagagca cattcgtttt tgatacttga aagtcgtgat
    12181 attctcacga ctttttatta gctttttgga ccaatacgtg tttttgacta gaatcttctt
    12241 tttaatgatc aaacgaagaa gtagtaacag tgttttttgc tattgagggg atagttattt
    12301 tttcaaaaat cttttccaaa aggatttggt tttttcagga gtttgtttgc taagttgatc
    12361 gttttttgtc tgctttaaga tgacttctaa acgtgtttcc ttcagattta ttgcgtggtc
    12421 ggtatgaagg ataaacgcaa aaggtgaagt catatgcgtt tctgtaataa tagtaacagg
    12481 taaatggttt tcttttgcta atttcatata gagtatttga agttgaggtg ccagcttgga
    12541 agagattttt aagtgtaaag gttgatagac taactgaagg ctcggtaatt ctcttttcaa
    12601 atagtctttt actgttttag tttgagcttc gtctaataaa gcacttaaaa cgactcgttc
    12661 agcatacgtt cctagatagt agcgttgctg ctcaggattt aatcttcttt ctccccaggc
    12721 tccttttaat agcttatctt ctaaattagt catagatacc ctcgcttctt tataatagtt
    12781 tctttattat atcgatttct acaataaaat accaatttga aaattttcgg taaatgtgtt
    12841 gaaaaacaag aaagaaagtg atttttcttg caaaaaacaa aggaagcttt tcatgagatt
    12901 gctttttaag ttgattaaag gcctagtttg tggtattatg aacatgtaaa atattaaaac
    12961 tcataaggag agtattacaa tgtcaattat tactgatgta tacgctcgcg aagtccttga
    13021 ctcacgcggt aacccaacac ttgaagtaga agtttataca gaatcaggtg cattcggacg
    13081 tggtatggtt ccttcaggag cttcaactgg tgaacatgaa gccgttgaac ttcgtgatgg
    13141 tgacaaatct cgttaccttg gtctaggtac tcaaaaagca gttgataacg ttaataacat
    13201 tatcgctgag gcaatcatcg gttacgatgt tcgcgatcaa caagctatcg accgtgcaat
    13261 gatcgctctt gacggtactc ctaacaaagg taaacttggt gctaatgcta ttcttggtgt
    13321 ttctatcgct gttgctcgtg cagctgctga ctatcttgaa gtgccacttt acacttacct
    13381 tggcggattc aacactaaag ttcttccaac tcctatgatg aacatcatca acggtggatc
    13441 acactcagat gccccaattg cattccaaga gttcatgatc atgccagttg gtgcacctac
    13501 tttcaaagaa ggtcttcgtt ggggtgctga agttttccac gctcttaaga aaattcttaa
    13561 agaacgcgga cttgttacag ctgtaggtga cgaaggtgga tttgctccta aatttgaagg
    13621 aactgaagac ggtgtagaaa ctatccttaa agctattgaa gcagctggtt acgaagctgg
    13681 tgaaaacggc attatgatcg gttttgactg tgcatcatca gaattctacg acaaagaacg
    13741 taaagtttac gactacacta aatttgaagg tgaaggcgct gctgttcgta catctgcaga
    13801 acaagttgat taccttgaag agttggttaa caaataccca attattacta tcgaagatgg
    13861 tatggatgaa aacgactggg atggttggaa agttcttact gaacgcctag gcaaacgtgt
    13921 tcaattggtt ggtgacgact tcttcgttac aaacactgaa taccttgctc gtggtatcaa
    13981 agaaaatgca gctaactcaa tccttatcaa agttaaccaa atcggtactt tgactgaaac
    14041 gtttgaagct atcgaaatgg ctaaagaagc tggatatact gccgttgtat cacaccgttc
    14101 aggtgaaact gaagattcaa caatcgctga tatcgcagtt gcaacaaacg ctggccaaat
    14161 caaaacaggt tcattgtcac gtacagaccg tattgctaaa tacaaccaat tgcttcgtat
    14221 cgaagatcaa cttggtgaag ttgctcaata caaaggtatc aaatcattct ataacttaaa
    14281 aaaatagacg gtaagacaga aatcggtcat tcgtagaatg cgatttcgtc gtcgcacctc
    14341 cgtcaggctg actaggttgg tcacgacaaa aggagtgaac atccagtcag ctacgacgca
    14401 aaagttaaag aatagaaagc ttgggaaaat cccaagcttt ttgctttcta aatagtgaaa
    14461 atttactgtg ggctaattta tcctgagcta atgtattagc gaggtaaagt tattgaggaa
    14521 ggcttggatt tgtactgacc ccaaaaagtt ggacactata ttttaagtat agaactaagt
    14581 ccttttatac taattgtcaa gcacatcaag gttttaactg aaaaaatgtt ttagacaata
    14641 gcctaggttt agctgttgtc ttacttgttg tgctctgatt agcataaaaa agtgtacaag
    14701 aaaaaaacct tgcctagtat ttttaagatc aaggtgttac aatgatagag caacaacgac
    14761 tttaatgatt ttgggtcgaa gcgtaattgt aaagtttgtt atgcgttatg aggtaaagca
    14821 ttgtcctgac aaggcaatgt ctagaggcaa tcgtatgtgg cttggttgaa gctgtttacg
    14881 attgcctttt tcgtttctca tagaagtctg cgatatgaca aggattggta tgactagcag
    14941 aagcaatgtc atggatacac ttgaaaagaa tctttggagc ataaggattc ccacgttcgg
    15001 taatgtgctc ttgagctaga cagttgccag actcataatg tctaaagtca atcccaatga
    15061 aagcattaat ttgattagca gattgaaaac aacgaatagc accgagttcg ccaataatac
    15121 ttgttgcagt tgtttctgcg atacttggaa tagagcgtag aatcttgtct tctggtaaag
    15181 gttgagccaa cgccaccatc ttatcaaaaa tagcttgacg acgttcagaa agtctaagta
    15241 attctttggc atagtagcga acctcttcta tcatcggaga ggttttcgta acagtaaaat
    15301 aagactgctt ggctaatgag acaagttttt ccactaagta gatcccacgc ttgtcagaga
    15361 tacgtttaga ggtagattga cggatgatag ctgacaatgc tttgtcagct agctccagta
    15421 caaagctatt acatgaaaaa agccgttacc acattccagt attactcacc agttggtgca
    15481 aataagatgt gttccagttc aggaaaagtg acttgtaaga tcttctgaag gcgattctta
    15541 actctcgaac aatattatcc tcagtcaagt tttgatagaa gcgactcaag tcacgtagca
    15601 gctgataaac ttcttcctga acgtaagttg gttttctaac tagaataaat tgagactgag
    15661 ccaatttttc agtgtctaac ttatccgctt tacgcacccg tagactatct agttttctaa
    15721 aggattgagt tgtgtataag cgtagccatt tttctcaagg aaggactgaa gccgacgaga
    15781 atagactcct gttgtgatgg tctccctttt gccttttaaa caatagtata gccgtttttc
    15841 aaaatgttat taatgatttt ttcctttatt ccagaagaat aagtctgata ctgtttctta
    15901 ttaataatct caatgcccta acaatccatc agtcgaacgg tatttctcaa attactgcag
    15961 ttgactcttg cgttcagttc cctcaccttg tggatgccgt ttagaacaat attagcttgc
    16021 tgagcctttt ggtaatgtga acaaagcatc tttatcactc aaggagaaga caggttcttg
    16081 tggtaagtgt ccaacaaggt cacgataaac tcatcgtacc gtttttggcg gaagtctaga
    16141 aataccttat gaatgtaagc ctcagaaacg tctctaaaac taaacaagaa atacaagaac
    16201 aaatgccctt gattctggta caaatgctgt atcaggttca ggtattgcct atctgcgata
    16261 atgacactct gtttttcctt aacaatatct ttgaaaggaa tgatttcgac attgacaggc
    16321 ataaccgttg caatagctgg cgggaagtaa atcgagagtg cattggtcat caaatccagc
    16381 gcagcagtgt tattggtcaa aagatagaca ggtattggtg gcaaatatgt ttttaggagt
    16441 tcctctaact gtatcgtgaa gagcagtaaa tgataggacg gcagtttccc cacgatgcct
    16501 tctgcgctca tccattcctc aacgatggtt tttagagtat tgtagaagct cttgtttcgt
    16561 ttttgctgtc ttctgaggta atggaacaag tgtatatcag gtaacaaaac ttgaagatta
    16621 ctgataaaga gacgtgagaa ggaaaccaaa atccgtttga aaggaaggga gtttaggata
    16681 ttatcgccta gtagtggacg cagggatgct ttcaaggttt ttccatttgt ttttgtcatc
    16741 accaactcaa acaattcttt cactttttta ggtgtccatt tatccttatg gaaaggatta
    16801 ttggtggtgc aaaaacataa gaaaatataa tccaaatcct catcagttaa atcaggatgg
    16861 taccattttc cgatgatttc ctgactggtc attttgatat cgtgatagat agacagtttt
    16921 ttcaagtgtt tgaatgcatc tgtctgaggg atccccaagt taccatgttg ccgtttccat
    16981 ataagggata tcagaatggc aaagtatgaa aattaataag gggctttact ggcatcatta
    17041 agtgtgatgg cttgattgga gtttataatc agcgaaacca ctttattcat tgatgtttta
    17101 tcaaaatcat aaatggtcat accaaactga tagtgcaaca aggcgatcag aaagcggata
    17161 cggtattctg gtccaacaac atggttttgc cgaacgttta aacctacctt tttcaagtat
    17221 ttccgacaat tggtcctgat acggtaagcg gtggcttggg aaatatactc tcgtttaatg
    17281 aagtcgtgta ggctcagctc tcctggttca attaaaaaac ataacatctt taagaatatc
    17341 gaggtttgat agagtttttt aaaataaggc attttaagtt ggtaggtctt ataggggata
    17401 gaaacagtgt cgtcacaaat ggttaaggtg agggtgtctt ctaaatcctt aatcatttca
    17461 atgagtaagt tgtagacacg ttgttttttg agttcggttt cctgacacaa ctggtctatg
    17521 gagacttcgg aaacgtctaa caaaatgttg agcagtgata atttatcttg taatttggtt
    17581 tctagatgaa ggtgtaatat aactgaactc ctttcatttt tatagcagaa actcgtattt
    17641 atttaattat cacataatta aataaaacag tcaaataatt caatggtgac gcgtgtttaa
    17701 ggagtattga gaatgtcaga tataagcaaa atgcagctga atattcttaa ttttaggtat
    17761 gaaaaagcgt cgggattgat aaaaaatcta ttgaattggg taaatttaag ttgatataat
    17821 aagttgatta gaaaaattaa taattcattt aaattctaat cagctgtaac gttccggggc
    17881 gtgttaaact gtctgaacat agaaaataca ggtttctacg gtgactaaag taagttaagg
    17941 aggattcctt aaattctgtg ataggaacgt aaactctcta caagagtaga gcacgtcatc
    18001 agtcagtctg ggtgtctact ggctgacaac gtcaaataag ataggcatga aaaaaataag
    18061 ggggaacaca gaatgttttt aaaacaccaa gatgtgaaac aaaaaaattg gagaatgcgt
    18121 aaggtcaaaa aactctttgt tagttcatgt atgcttttaa cagtcggcct cggagttgcc
    18181 gtacctactg gattcagcca atctaatggc gtgatggtcg taaaggctgc ggaagtgccg
    18241 gcggctgagg gagtttttaa aatagagacc gataacttgc aaaactggga agcagagact
    18301 aatactcaag gttataaagg aactttttca aaatcgccag actctcaagg attttataaa
    18361 gttcaaacca actcaggagc tggtataggt gttcaagttg ctcctaaggg aaatggcaga
    18421 atggacatta agattggtaa tcgagttgaa ataccaaaag gttattatgt tggtggaatc
    18481 agaactaaga cgccagtttt ggaaaaagat aatacttatc gtttatcctt tacgactagt
    18541 aagacttctg gaacagatgt agaattcaaa acgcgtataa aagatgttga ggcaaatggt
    18601 gacttaggcc aagagcatac aatcaaattt gatggtaata cagtatctac aggtaatcct
    18661 tggataaaag gtctaaccaa caaaaaacat actgttgatt ttattggtaa aggaaaagcg
    18721 ttgtcaatgg cttttaggtt ttctcctttt aaaaccacca atataacata tacttttaca
    18781 gatatgaata tttctaatgt gactcctgca tcagtccccg aaacaccttc ttcagtttat
    18841 gaagaggcaa ctaaaatttc tggtaaagca gtggcttcta aagacaattt taataaaaga
    18901 acgaactttt taggtgatgt tgtgaatgtt tataagttgg atcagttagt tggaactgcg
    18961 caagttaaat cgggaaatac ttttgaagtt gttttgaatt taaaagaacc tttgaaacaa
    19021 ggtgaaaaac ttgaagttga aattcagaat cctaaatcag ctttattgag tgcgcgtgta
    19081 ccaatcaacg tgatagcaaa accttatgat aaagcccaac acttaaaaga ttctaaagcg
    19141 agtctagatg aagcttatcg tcaaactgta gaaaatataa ctcaagataa ttggttaaaa
    19201 tcttcagaaa aacaaaaaca aaccaacgct gcaaagttag catatgacca agggattatc
    19261 gaactaggga gaagcaacga taaagctgca attaattctg tggttacaaa atatacgcaa
    19321 aagaaaactg ggctaattac taaagaacat attttaggtg aaaaagataa ggtaattact
    19381 gcagcaaaga atgctattga agaaaaagca acggcagaaa aaacaaaaat tgattctgat
    19441 aacagtttaa caactccgga aaaagaagca cagttagccc agttgtcaac agcaaaagaa
    19501 gcggctttaa ctgctattac agagtctccg gatgcagata ctgtagggac taaacaaacc
    19561 gaaggtgaag cttcagtatc agggattcat caaggtaaag atttagaagc tattaaaact
    19621 gcagcaaaga atgctattga agaaaaagca acggcagaaa aaacaaaaat caataacgat
    19681 atcacattaa cggcaaaaga caaagaacaa caactgaaag aagttgagac agctcttact
    19741 aaagctaaag ataacgtgaa agctgctaag acagcagacg ctatcaatga cgcacgtgat
    19801 aaaggcgtag caacaattga tgccgtccat aaagcaggtc aagacttagg tgctcgtaag
    19861 tcaggtcaag tcgctaaact tgaagaagca gctaaagcaa cgaaagacaa gatttcagct
    19921 gatccaactt tgacaagcaa agaaaaagaa gagcaatcta aagctgttga cgctgaactt
    19981 aagaaagcga tagaagctgt taacgcagct gacacagctg acaaggttga cgatgctctt
    20041 ggtaaaggtg tcacagacat caagaaccaa cacaagtcag gtaaaggtct tgacgttcgt
    20101 aaagatgagc acaagaaagc tcttgaagct gttgctaaac gtgttactgc tgaaattgag
    20161 gctgatccaa ccttaacacc agaagtgaga gaacaacaaa aagcagaggt tcaaaaagag
    20221 cttgaacttg cgactgataa gattgctgaa gctaaagatg cagatgaagc agacaaagct
    20281 tacggtgacg gtgtcacagc gatcgaaaat gcccatgtta ttggtaaagg tatcgaagct
    20341 cgtaaagacc ttgctaagaa agaccttgct gaagctgttg ctaagacaaa agctctcatt
    20401 attgaagaca aaacgcttac tgatgatcaa cgtaaagaac agttatcagg tgttgataca
    20461 gagtatgcta aaggtatcga gaatattgat gcagctaagg atgctgcagg tgttgataaa
    20521 gcatatagtg acggtgttcg tgacatcctg gcacagtaca aagaaggtca aaaccttgat
    20581 gatcgtcgta atgctgccaa agaatttctt cttaaagaag cagacaaagt gacgaaacta
    20641 atcaatgatg atccaacctt gactcatgac caaaaagttg atcaaatcaa caaagttgaa
    20701 caagctaagt tagacgcaat caagtctgtt gatgatgctc aaacagctga tgctatcaat
    20761 gatgctcttg gtaagggtat tgaaaacatc aacaaccaat accaacatgg cgatggcgtt
    20821 gatgttcgta aagcgactgc caaaggcgat cttgaaaaag aagctgctaa agtgaaagct
    20881 cttattgcta aggatccaac cttgactcaa gctgataaag acaaacaaac agcagcggtt
    20941 gacgcagcta agaatacagc aat
//
LOCUS       DAUELZ010000020        20808 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.020, whole genome shotgun sequence.
ACCESSION   DAUELZ010000020 DAUELZ010000000
VERSION     DAUELZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20808
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.020"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..87
                     /locus_tag="VHI09_000588"
     CDS             <1..87
                     /locus_tag="VHI09_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEQ6120485.1"
                     /translation="LFLEPRELYDFPQYILDEDYFLITKNMV"
     gene            357..803
                     /locus_tag="VHI09_000589"
                     /pseudo
     CDS             357..803
                     /locus_tag="VHI09_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370026.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            873..2705
                     /gene="lepA"
                     /locus_tag="VHI09_000590"
     CDS             873..2705
                     /gene="lepA"
                     /locus_tag="VHI09_000590"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEQ6120486.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDTKK"
     gene            3019..4020
                     /locus_tag="VHI09_000591"
     CDS             3019..4020
                     /locus_tag="VHI09_000591"
                     /inference="COORDINATES: protein motif:HMM:NF013550.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164232585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ6120487.1"
                     /translation="MLTFGGASAVKADAVGRQQVQDELQNQRKALPRLKEFYKDTPNS
                     GVENTPIYKYLTEMEGKLNRYLETLDKGLHQLQGDKGEPGAQGPAGPRGETGPAGERG
                     EKGAQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGTQGAKGDRGETGP
                     AGPRGDKGEKGEQGPAGKDGERGPIGPAGKDGQNGKDGLPGKDGKDGQNGKDGLPGKD
                     GKDGQDGKDGLPGKPAPQTPEVPQKPDTAPHTPKTPQIPGQSKDVTPAPQNPSNRGLN
                     KLQTQGGNQLAKTPAAHDTHRQLPATGETTNPFFTAAAVAIMTTAGVVAVAKRQENN"
     gene            4206..4643
                     /gene="msrB"
                     /locus_tag="VHI09_000592"
     CDS             4206..4643
                     /gene="msrB"
                     /locus_tag="VHI09_000592"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ6120488.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGKYDDFFEKGIYVDI
                     VSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYDQMEKGGYAQWLTLFDET"
     gene            4773..5792
                     /locus_tag="VHI09_000593"
     CDS             4773..5792
                     /locus_tag="VHI09_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEQ6120489.1"
                     /translation="MSTNLRKLPGLLLCLLLALPAWYLGRLFPIIGAPVFAILLGMLL
                     ALFYEHRDKAKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGSQKWLGLDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNEGFAIFAGTAVNDTSSVTATATAWDALHHSNTLDGATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEEGFSLRKSFPRFILFFLLASLITTLMTSFGVS
                     ADVFHSLKTLSKFFIVMAMVAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            5999..6424
                     /locus_tag="VHI09_000594"
     CDS             5999..6424
                     /locus_tag="VHI09_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ6120490.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDDFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPDEALTKDEIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            6451..6942
                     /locus_tag="VHI09_000595"
     CDS             6451..6942
                     /locus_tag="VHI09_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ6120491.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRATDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            6959..7768
                     /locus_tag="VHI09_000596"
     CDS             6959..7768
                     /locus_tag="VHI09_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ6120492.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAINKQTTFDSKESEEEDFFA"
     gene            7765..8592
                     /locus_tag="VHI09_000597"
     CDS             7765..8592
                     /locus_tag="VHI09_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ6120493.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            8728..10377
                     /locus_tag="VHI09_000598"
     CDS             8728..10377
                     /locus_tag="VHI09_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ6120494.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDELGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPNQGLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTVTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            10381..11169
                     /locus_tag="VHI09_000599"
     CDS             10381..11169
                     /locus_tag="VHI09_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ6120495.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            11163..12209
                     /locus_tag="VHI09_000600"
     CDS             11163..12209
                     /locus_tag="VHI09_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ6120496.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNVAMYIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSVVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            12324..13721
                     /locus_tag="VHI09_000601"
     CDS             12324..13721
                     /locus_tag="VHI09_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEQ6120497.1"
                     /translation="MAYQTIYPYTNEVLHTFDNMTDQGLADVLERAHLLYKKWRQEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVDLCAGIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFASNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEEGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGIKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            13823..15619
                     /gene="uvrC"
                     /locus_tag="VHI09_000602"
     CDS             13823..15619
                     /gene="uvrC"
                     /locus_tag="VHI09_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEQ6120498.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLRIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            15803..16405
                     /locus_tag="VHI09_000603"
     CDS             15803..16405
                     /locus_tag="VHI09_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEQ6120499.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIEIR"
     gene            16530..17939
                     /gene="pepV"
                     /locus_tag="VHI09_000604"
     CDS             16530..17939
                     /gene="pepV"
                     /locus_tag="VHI09_000604"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEQ6120500.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDRLADDQHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNKGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLDVLEAALEQFLSEHGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGVNGATLLAKFLNQFTFEGAAKDYLHVAGEVLHEDFAAEKLGLAYTDDRMGALSMNA
                     GVFTFDSQSADNTIALNFRYPKGTDAATLKSGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVTTLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(18007..19383)
                     /gene="mnmE"
                     /locus_tag="VHI09_000605"
     CDS             complement(18007..19383)
                     /gene="mnmE"
                     /locus_tag="VHI09_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEQ6120501.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPNTGTIVDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQDSNRI
                     ILLNKTDLEQKIELEQLPADLISISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     misc_feature    19555..19690
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            19719..20219
                     /locus_tag="VHI09_000606"
     CDS             19719..20219
                     /locus_tag="VHI09_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEQ6120502.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            20284..20649
                     /gene="rplL"
                     /locus_tag="VHI09_000607"
     CDS             20284..20649
                     /gene="rplL"
                     /locus_tag="VHI09_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEQ6120503.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
BASE COUNT         6387 a         3793 c         4446 g         6182 t
ORIGIN      
        1 ctctttttag aaccacgtga actatatgat tttccgcagt atattttaga tgaagattat
       61 ttcttaataa ctaaaaatat ggtataatat tcttaataaa tgcagtaata caggggcttt
      121 tcgagactga agtctagctg agacaaatag tgcgattacg aaatttttta gacaaaaata
      181 gtctacgagg ttttagagct atgctgtttt gagtggtctc cattcaacat tgccaatgat
      241 aacttgagaa agagggttca taccagcagt cggatacctt catattcttt ttgttaaagc
      301 gttttcatgt tataataggc aaaagaagag tagagtgatg gaaccaacat ttttttatga
      361 ttaagccaga tggggttaag cgagagggtt agttggagag attttgcggt ggattgaacg
      421 cctaagattt acgtttgagc gattcgagct aagacaagct agttcgaaat acttggctaa
      481 gcacgacgag accttggtga taaacctttt gatcctaaac ttaaagctta catgacaagt
      541 ggtcctgttt taattgggat aattcttggg gactaaggtg gttttgtctt tttgaaccat
      601 gcatgatgag agtaaccaat cctacggatg ctctttgcgg aaccattcgt gggaattttg
      661 ctcaggctct tggtgatgat ggaggtattt ttaacatggc gtatgggtct cattcaagag
      721 actctgctag gcgtgagatt gtcctttggg ctcatcaaag taatctcggt agctccgcta
      781 ttctcttgca agataatccg taagaaaaga taggcaaagg cctacctttt ttgttataat
      841 agaaggaaag actaaatgta taggaaaata gaatgaacag tcaagattta aaaaaacgtc
      901 aggagaagat tcgtaatttc tccattattg cacatattga ccatggcaaa tctactctcg
      961 ctgaccgcat tttggaaaag acagagacgg tttcgtctcg tgaaatgcag gctcagttat
     1021 tggactccat ggatttggag cgtgagcgtg ggattaccat taagttaaac gccattgagc
     1081 ttaattacac ggctaaagat ggggaaacct acattttcca ccttattgat accccagggc
     1141 atgtggactt tacttatgaa gtatcgcgtt cgttggcagc ctgtgaggga gcgattttag
     1201 ttgtggatgc agcgcagggc attgaagcgc agacccttgc caatgtttac ctagctcttg
     1261 ataatgattt ggaaatttta ccggttatca ataaaattga tttgcctgct gctgaccctg
     1321 agagagtccg ccatgaagta gaagatgtca ttggacttga tgcttctgag gctgttctgg
     1381 cgtcagccaa ggctggtatt gggatcgaag agattcttga gcaaattgtt gagaaggttc
     1441 ctgctcctac gggtgatgtg gatgcgcctt tacaggcctt gatttttgac tctgtgtatg
     1501 atgcttaccg aggggtaatt ttgcaggttc ggattgtgaa cggtattgtc aagcctggcg
     1561 ataaaatcca gatgatgtcg aatggaaaaa cctttgatgt tacagaagtt gggattttca
     1621 ctcctaaggc agttggacga gatttccttg cgacgggaga tgttggttat gttgcggctt
     1681 ctatcaaaac ggtagcagat acccgtgtgg gggatacggt gactttagct aataaccctg
     1741 ctaaggaagc cttgcatggc tacaagcaga tgaacccgat ggtctttgca gggatttatc
     1801 cgattgaatc aaataaatac aatgacttgc gtgaagcgct tgaaaaatta caactgaacg
     1861 atgccagttt acagtttgaa cccgaaacgt cacaagcgct tggctttggt tttcgatgcg
     1921 gctttttagg tttgcttcac atggacgtga ttcaagaacg tttagagcgt gaatttaaca
     1981 ttgatttgat catgacagca ccgtccgtgg tgtatcacgt tcacacaaca gatgaggata
     2041 tgattgaagt gtctaaccct tcagaatttc cagacccaac ccgtgtggct tttattgaag
     2101 agccttatgt taaagcgcaa atcatggtgc cacaagagtt cgtaggggct gttatggaat
     2161 tatcgcagcg caagcgtggt gattttgtga ccatggatta cattgatgat aaccgtgtca
     2221 atgtgattta ccaaatcccg ctggctgaaa ttgtctttga tttctttgat aaattgaaat
     2281 cctcaacgcg aggctatgcg agttttgatt atgacatgtc agaataccgc aggtcacaat
     2341 tggtgaaaat ggacattctt ttgaacggtg ataaggtcga tgcgctcagc tttattgttc
     2401 ataaagaatt tgcttatgaa cgcgggaaaa ttattgttga gaaattgaaa aaaatcattc
     2461 cacgtcaaca gtttgaagtg ccgatccaag ccgctattgg tcaaaaaatt gttgcgcgtt
     2521 cagacatcaa ggcccttcgt aaaaacgttt tagcgaagtg ttatggcggt gacgtgtctc
     2581 gtaaacgtaa attgctcgaa aaacaaaagg ctggtaaaaa acggatgaag gctattggtt
     2641 ctgtcgaagt ccctcaagaa gccttcttga gtgttctttc aatggatgac gataccaaga
     2701 aataaccaag cttaatcgct tagtcttcag aggagaactc ttgtgggtct acatgaccaa
     2761 aaaattgatt atctcataaa aaaaagaact ttacaatctc tttcacataa cactataatg
     2821 gtattatagt gttatctcat tattaggtga gcctgcattt aatcttatta aagagaaaga
     2881 ggaaacaaaa accgtgaaca aaacaaaaca aaacaaaaca aaacaaaaca aaacaaaaca
     2941 aaacaaaaca aaacaaaaca aaacaaaaca aaacatagcc ttctttgtcg ctatggctta
     3001 acctcagcag ctgctctttt gctgaccttt ggaggtgcaa gtgcagttaa ggcggatgca
     3061 gtaggaagac aacaagtgca agatgaactt caaaatcagc gaaaagcttt accacgtctt
     3121 aaagaatttt ataaggatac tccaaactcc ggtgttgaaa atactccgat ttataagtat
     3181 ttaactgaaa tggaagggaa gttaaatcgt tatctggaga ctttagacaa agggttgcac
     3241 cagttgcaag gtgacaaagg agagcctggt gctcaaggcc cagcaggtcc acgtggtgag
     3301 accggtcccg ctggtgagcg aggtgagaaa ggagcccaag gtccacgtgg tgaacaaggc
     3361 ccgcaaggtc ttccaggtaa agatggtgaa gctggtgctc aaggcccagc aggtccaatg
     3421 ggtcctgctg gtgagcgagg tgaaaaagga gaacctggta cccaaggcgc taaaggtgat
     3481 cgcggtgaaa ccggcccagc aggcccacgt ggtgacaagg gtgaaaaagg agaacaaggt
     3541 cccgccggta aagatggtga acgtggtcca ataggtccag ctggtaagga cggccaaaac
     3601 ggcaaagatg gtctcccagg taaagacggc aaggacggcc aaaacggtaa agatggtctc
     3661 ccaggtaaag acggcaagga cggccaagat ggcaaagatg gtcttccagg taaaccggct
     3721 cctcaaacac cagaggtccc tcaaaaacca gatactgcac cacatactcc aaaaacccct
     3781 cagatccctg gccaatcaaa agacgtgaca cctgctcctc aaaacccttc taatagaggt
     3841 ctaaacaaac tacaaacaca aggtggtaat cagctcgcaa aaacaccagc agctcacgac
     3901 acacacagac aattgccagc aacaggcgaa acaaccaatc cattctttac agcagctgct
     3961 gtagctatca tgacgacagc tggagttgta gctgttgcaa aacgtcaaga aaacaactaa
     4021 tcctcttttt gctacctaac tgatggaaag taaaactcag agagaacctc aaggttctct
     4081 cttttttgaa tcaagatagt agctctaggt ataaagtctt gtctgaggtg ctgtgaacaa
     4141 atcagttacc cttcacctct ttttttggta aaatgtaagc actaaaacat caaaaggagt
     4201 tttctatgga aacatcagat gagttaaaac aacgcattgg tgatctttct tacgaggtca
     4261 cccagcatgc agcaacagaa tctcccttta cagggaaata tgatgatttt tttgagaagg
     4321 gtatctatgt cgatatcgtc agtggtgagg tcttgttttc atcactggat aaatttaatt
     4381 ctggctgtgg ttggcctgcc ttctcaaaac ctattgaaaa tcgcatggtc acgaaccacg
     4441 atgactcttc ttatgggatg agacgagttg aggttaaaag ccgtgaagca ggatcgcatt
     4501 tggggcatgt ctttagtgac ggccccaaag aagctggagg cttgcgttac tgtatcaatt
     4561 cagcagctct taaattcatt ccttatgacc agatggaaaa aggaggctat gcccaatggc
     4621 tcacattgtt tgatgagaca taacagggtt tcaaaatgac taaaaaagaa gcgtcagtga
     4681 ttcccataag cactggattg gtgaggcgta cgggtcaatt ttacaagcct ttgtaaaagg
     4741 tctataatag ggaatgaaaa aaggaggtta gtatgtcaac taacttaaga aaacttccag
     4801 gcctgttact ttgcttattg ttagctcttc cagcctggta tttagggcgc ttatttccca
     4861 ttattggagc acctgttttt gctattcttt taggaatgtt gttagccttg ttttatgaac
     4921 atcgtgacaa ggctaaagag ggaattagtt ttacatccaa gtatattttg caaacagcag
     4981 tggttttgct tggttttgga ttaaacctaa cccaagttat ggcagtaggc atgcagtctt
     5041 tacccattat catttcaact attgcgacag ctcttttggt agcttatggc tcacagaaat
     5101 ggctgggctt agatgtcaat acagccacct tggtgggtgt agggtcttcc atttgtgggg
     5161 ggtctgctat tgcagcgacg gctcctgtca ttaaggcaaa ggatgatgag gttgctaagg
     5221 caatttcagt catttttctc tttaatatgt tagcagcttt gctatttcca agtttaggac
     5281 aattactggg cttatctaat gaaggttttg ctatttttgc tgggacagct gttaacgaca
     5341 cctcttccgt gactgcaacg gccacggcct gggatgccct tcaccattcc aatacactag
     5401 atggcgcaac cattgtcaaa ttgactcgca ccttggctat tcttccaatt actttaggtt
     5461 tatcccttta ccgagcgaaa aaagagcacg acatcgttac agaagaaggc ttcagcctta
     5521 ggaagtcatt ccctcgtttc atcctcttct ttttattagc ttccctcatc acgacattga
     5581 tgaccagctt tggtgtttct gccgatgttt tccattccct aaaaacctta tcaaaattct
     5641 ttatcgtgat ggctatggtg gctatcggtt taaatacaaa cctggttaaa ttaatcaaga
     5701 cgggcggtca ggctatcctt ttaggagcta tctgctgggt agctatcacc cttgtcagtt
     5761 tagccatgca attaagtttg ggcatttggt aatcagacac gagaaatcag tttgactgat
     5821 ttcttttttt gtatcttatt tgtgtagaat tgtctacatt ttaaagagaa tagtgcattt
     5881 taaaaacgtt ttcatttgat aaaataaacc tatcgttaaa ccagttcagt aaagacaact
     5941 ggttgagata agcaattacc gtttattcag cggtttttgt taatagagga ggataggaat
     6001 gaaacgaaaa tttctcattg ggagtcacgg cagactagcc agtggtttgc aaagctctat
     6061 tgacatttta gcaggtatgg gacaggcact tgaaaccatt gatgcttatg ttgatgatag
     6121 tgactacact agccagattg atgactttat cgctggggta gcagcagatg agcagggcct
     6181 tatttttact gatttgttag gcggcagtgt gaatcagaaa atggtaaccg ctgtaatgaa
     6241 cagtggcaaa gacaatatct ttttaatcac aaacagtaac ttagccactt tattgtcgtt
     6301 ggtgttttta aaaccagacg aagcgcttac taaagatgaa attgtaaccg ttatcaatga
     6361 atcgcaagtt cagttagtgg atttggtccc tgagacaaat tcagaagatg acttttttga
     6421 ttaatatctt aatatctagg aggaagacaa atgattactc aaattcgtgt tgacgaccgt
     6481 ttgattcacg ggcaagtggc agttgtatgg accaaagagt taaatgcacc gcttttagta
     6541 gtggctaatg atgaagctgc aaaaaatgaa atcacacaaa tgacgcttaa aatggctgtg
     6601 ccaaacggca tgaaactgct tattcgttca gttgaagact cgattaagct ctttaatgat
     6661 ccacgcgcaa ctgacaagcg tatttttgtg attgtcaatt ctgtcaaaga tgcctgcgcc
     6721 attgctaaag aagttccaga cttagaagct gttaatgtgg ctaatgtggg gcgttttgac
     6781 aaatcagacc cagcaagcaa agtcaaagtg acaccaagtc ttttacttaa cccagaagaa
     6841 atggcagccg caaaggaatt agtcagttta ccagaacttg atgtctttaa ccaagtctta
     6901 ccatcaaata ccaaggtcca tttgagccaa ttagtcaact aaagagaggt aaaaaagtat
     6961 gttagtacca gcaacaatgg cagctttagc tgtattgatt tgttttgggg gaaattattt
     7021 aaccggtcaa agcatgatgg aaagacccct agtagttgga ctggtcacag gtcttctttt
     7081 aggagatatg aaggtcggta tcttaatggg ggcctctcta gaagctcttt tcctaggaaa
     7141 tgtgaatatt gggggtgtta ttgcagctga gcctgtaaca gcgactgcca tggcaacaac
     7201 ctttaccatt atttcacata ttgatcaaaa agcagccatg accctagctg ttccaattgg
     7261 aatgttggca gcctttgtgg ttatgttttt gaaaaacgtc ttcatgaata tctttgctcc
     7321 aatggttgat aaagctgcag cagctaacca tcaaggtaaa ctagtgatgc tccactatgg
     7381 cacttggatt atctattact taatcattgc ctctatttct ttcattggta ttttagtagg
     7441 tagtggacct gttaatgcct ttgtggaaca cattcctcaa aatctcatga atggcttgag
     7501 tgctgcggga ggccttcttc cagctgtcgg ttttgccatg ctgatgaaat tattgtggac
     7561 caacaaatta gctgtttttt acctattggg ctttgtctta acagcttatt tgaaattacc
     7621 agcggttgca gttgctgccc taggagcagt tatttgcgtc attagttccc aacgtgatct
     7681 agaattagac gcaattacta gaggtgcaat taataaacaa acaacctttg actctaaaga
     7741 atcagaagag gaggacttct tcgcatgaca tcacaagata atttgactaa agaagaccgt
     7801 aaaatgcttc gctcagtctt ttggcgctca tggacgatga atgctagtcg tactggggca
     7861 acacaatacc acgcagtagg ggttatttac actttactac cagtcatcaa tcgtttttat
     7921 aagactgata aggataaagc agaggcactg gtccgtcata caacatggtt caatgccacc
     7981 atgcacatca ataactttat catgggtctt gtggcatcga tggaaaagaa aaacagtgaa
     8041 gatcctgatt ttgatgctag tgctatcact gctgttaagg cttcgttgat gggacctata
     8101 tctggagttg gagattcctt cttctgggga attttaaggg ttattgcggc aggtatcggg
     8161 atttccttgg caagtgcagg ctcagccatg ggagctgttg tcttcttact cttgtacaat
     8221 attcctgctt ttattattca ctactatagc ctttatggtg gttattctgt aggtgcagga
     8281 tttatcaaaa aactttacga atctggtggt ataaaaatcg tcactaaaac ctcaagcatg
     8341 cttgggttaa tgatggtagg ctcaatgact gcttcaaatg taaaatttaa aacgattctt
     8401 acagttgctg cgaaaggtgc caaagaagca gcttctattc aagattacct tgaccaattg
     8461 tttattggta ttgtaccatt aatggtaaca ctagcagctt tttggcttct acgaaagaaa
     8521 gtgaatatca actggattat gtttggtatc atgttccttg gcattatcct tggactttta
     8581 ggtatttgct aataatctcc tagcaataaa ggtaagtaag acaagttttc ttgttttgct
     8641 tacctttttc cttttaagat gatgagactt gttttgatga aaaacgtagt agcattaaga
     8701 aatttgttat aatgaaggga gcttaagatg cgaggtgaac aggtggaaga acattttaaa
     8761 aaacaattac aagatgatat ttctagacat ttttcgtacc aatcgttaat gttatcattg
     8821 ttgctaattg gtctttttat tattttttca ttagcgccac aacaattagg cctctatcgt
     8881 gatatcaatg ccactgcaac ccgttatcac cgtttgatta gcaaacaaga agccttgcta
     8941 gatgaattag ggaaaaatag cttactacct tttttaaata aaaacctcag cactgctgat
     9001 ttaagcaagc actattttca tttgcgccat agcagccaaa cgtcaccaga acttttacta
     9061 ttttcaccta atcaaggcct tttatttgct agtaacccac atttaggaaa tgtttttagt
     9121 aaatctgttt atattcaaga agtcttgaga gcagctcatc ctccaaaaac cttgtttaaa
     9181 gttgctatgg atagtgaaga tggtcactac ttgatgatca ttaagccaat gatagatcaa
     9241 aaccaactaa agggatatgc ttttttagtg atgagtggca aagattttct tcatcctaca
     9301 aaaacattga cgtcagagtt ggtcattgct gataagctgg acaacacggt tacgttttcc
     9361 aatcgtgagt ttatagcatc tagtctagat aagatcaata gccagtattt acaccattat
     9421 tttgtttttc aagataatcg agcctttatc acaagaaagg ttgccttaca aggaggcctt
     9481 tggctttaca tgtataggcc cttgattcca atggtatcgg tgatgttatt ttcacttatt
     9541 tcatcagctg ttatctttgt gattttgcaa cgtaaatcaa gtggtttagc taaccgaatt
     9601 gcagctaaaa attcaagagc aatcaatcaa atggttagag atatgagtgc gatctctcgg
     9661 caagaaaaaa gacgtattga ccttgagagt caagatgaat ttcaatattt atctgatcaa
     9721 atcaatcaaa tggtagagcg attgcaacag ttacatgata aaacgttaga tttggaaact
     9781 caaaaattat tatttgaaaa acggatgtta gaagctcaat tcaatccgca tttcctctac
     9841 aatacgctag aaaccatttt gattacgagc cattatgatt ctgccctaac agaaaaaatt
     9901 gttatccaat tgacaaaatt gttgcggtat agtctcacgg attctagcaa acctgtcttg
     9961 ctcaaagatg atttaagtgt catcgagtct tatttagtga ttaatcaggt gcggtttgaa
    10021 gaattgcaat atagcattaa cctatcccca gaccttgata gtcttgcagt gcctaaattg
    10081 ttcttgttac ctttgataga aaatgccatc aaatatggct tgaaagaacg acacgatgtc
    10141 aagattaaca ttgcttgtta ttatcaagat gaccacatta ttttttctgt gagagataat
    10201 ggttcgggaa ttgatgctca tcaccaaaaa gtgattcgag agcaattgga agctggagag
    10261 tcacaccatg ggttaattaa ctcttatcgt cggcttaagt atcatttttc agaagtatcc
    10321 ttggtttttg atcaaggtga taaacagttt aatgttagtt accatgttaa ggagtagtct
    10381 atgtacaaat tagtcattat tgaagatgaa catattatta ggaaatggtt gcgatacgct
    10441 attgattata aagctttaga tattttggtc attggcgagg ctaaagatgg caaagaagga
    10501 gctgttttaa tcaaagagag tcaaccagat atcgttttaa ccgatattaa catgcccatc
    10561 atgacagctt ttgacatgtt tgaagcaaca aaagatcaaa cttacgccaa aattatttta
    10621 tctggctatg ctgattttcc aaatgcaagg tctgccatac actacggtgt acttgaattt
    10681 ttaacaaaac cgattgaaaa agcagctctt tgggaatgtc ttcaaaccat tattgctaaa
    10741 atagaaaagc aaaaggggtc taatcaaaaa acagatgctt gcgtctatat tcccttaccc
    10801 cagatgactg atcagattcc tgaagtagtc aaagatattt tggagtgggt acatgctcat
    10861 tttcaagaca aaatcagcac gagccgccta gctcatgact tagggtatag tgagagttat
    10921 atctatcaaa atatcaaaaa gcatcttcag atgcctttaa gtgattacat taatcaatat
    10981 cggattaatc aggcaattca attgatgcaa caagaaccag atttaatggt atatgaaata
    11041 gcacaagctg taggtattta tgactatcgc tattttgata gggtttttaa aaagtatcta
    11101 ggacaaactg taaaggcttt taaagaagag cattttatga aagataccga taggagtaag
    11161 ccatgttaaa actcaaacgc aaatggctat taagcttttt gttagtaata attattctag
    11221 caatcgttaa cgttgcaatg tatatattct catcgtcaaa aaaagatagt gctaaagaat
    11281 tagttatctt gactcctaat agccaaacta ttttaacagg gactattcca gcctttgagg
    11341 aaaagtatgg ggttaaagta agattaatcc aaggtgggac gggccaactt atcgatcaat
    11401 taggtcgaaa agataagcca ttaaacgctg atattttctt tgggggcaat tacactcaat
    11461 ttgaaagcca taaagattta tttgaatctt atgtttctcc gcaggtttct actgtcattt
    11521 cagattacca attgcctagt catcgtgcaa ccccatatac gatcaatggc agtgtactga
    11581 ttgttaataa cgaattagca agaggacttc atattaccag ttatgaggat ttgctacaac
    11641 cagctttaaa aggcaaaatt gcttttgctg acccaaatag ctcatctagc gctttttcac
    11701 aattaacaaa catcttactg gctaaaggag gctatacgag ctcagaagct tgggagtatg
    11761 tgaaacgttt attactgtct atgaattcta tcagagcaac aagttcttca gatgtttacg
    11821 aatctgttgc agaaggtaaa atgattgttg gtttgaccta tgaagatcct tgcatcaatc
    11881 tgcaaaaaag cggtgcaaac gtttcggttg tttatccgga tgaggggaca gtatttgttc
    11941 cgtcatcagt cgctattatc aaaaaagcaa aggcaatgaa agaagctaag caatttatta
    12001 attttatttt atcatcagat gtacaaaatg cctttgggca gtcaactagt aatagaccta
    12061 tccgacgaga tgctcaaacg agtcatgaaa tgaaaccatt agatgaaatt attactttaa
    12121 aagaagatta cggatatgtt actagacata agaaaaaaat attgaatacc tataatcgac
    12181 tacatgaagc attagaaaga ggaaagtaga ctctaggctt gttgttgcta tactagtagt
    12241 gttacaaaat agcgtagtca ttttttgaaa tcgatttcat aatctggtag actaaggtta
    12301 tcacgaaaag gaggctatat gtgatggctt atcaaactat ttacccttat acaaatgaag
    12361 tccttcatac atttgataat atgaccgatc aaggtttggc agatgtcctt gaaagagctc
    12421 accttctcta taaaaaatgg cgccaagaag atcatttaga agaacgtaaa gcccagcttc
    12481 atcaagtggc taatattctt cgccgtgacc gcgataaata cgctgaaatc atgaccaaag
    12541 acatgggaaa attgttcaca gaagcccaag gtgaagtaga tctttgtgcc ggtattgctg
    12601 attactacgc tgataaggca gatgaatttt tgatgtctac gccactagaa acggattctg
    12661 gtcaagctta ctatttgaaa caatcaacag gtgttatcct agctgttgag ccttggaatt
    12721 ttccttacta tcaaatcatg cgggtttttg cgtcaaactt tattgttgga aacccaatgg
    12781 ttttaaaaca cgcttctatt tgcccacgct ctgcccaatc tttcgaagaa ttagttcttg
    12841 aagctggtgc tgaagaaggt agtattacca atttgtttat ttcttatgac caagtatcac
    12901 aagtgattgc tgataaacgt gtggttggtg tctgcttgac aggttccgaa cgtggaggag
    12961 ctagcattgc agaagaagct ggtaaaaact tgaaaaaaac aacccttgaa ttgggtggcg
    13021 atgatgcctt tattatcctt gatgacgcgg attgggatca attagagaag gtgctttact
    13081 tctctcgtct ctacaatgca ggacaagtat gtacctcatc gaaacgtttt atcgtgcttg
    13141 acaaagacta tgaccgtttc aaagagttat taacaaaagt cttcaaaaca gctaaatggg
    13201 gtgacccaat ggatcccgaa acgacgttgg cgccattatc atcagctcaa gctaaagcag
    13261 atgttcttga ccaaattaag ttggccttgg atcatggtgc ggagttggtt tatggtggtg
    13321 aagcgattga tcatccaggt cattttgtca tgccaaccat tattgctggc ctcacaaaag
    13381 acaaccctat ttactatcaa gaaatctttg gcccagtcgg tgaaatctac aaagtgtctt
    13441 ctgaagaaga agcgattgaa gtggcgaatg attctaacta tggccttgga ggtactatct
    13501 ttagtagcaa tcaagaacat gcgaaagccg tagctgctaa aattgaaact ggtatgtctt
    13561 ttatcaactc aggctggaca agtcttccgg aactcccatt tggtgggatt aaacattcag
    13621 gctatggccg tgaattgagt gagcttggct ttacatcttt tgtgaatgaa caccttatct
    13681 atattccgaa taaaacgaat aatagcaata cgaaagtttg atctagattg ttaaaggaga
    13741 tctgctcgcc ttaataaaca atcaaggcgt tacactcttc ttttttaaag tcaaaatttt
    13801 aaaaatttga taaactagta acatgaacga actgattaaa cataaactag aactcttacc
    13861 agacagccct ggctgttact tacataaaga taaagaagga actattatct atgtcggtaa
    13921 ggccaagaat ttgaaaaaac gcgtccgatc ctattttcgt gggagccatg ataccaaaac
    13981 agagttatta gtatctgaga ttgtagattt cgaatatatt gtaacagagt cagataccga
    14041 agcgcttttg ttggaaatta acttgattca aaaaaatatg cctaagtaca acatcaagtt
    14101 aaaagatgat aaatcttatc cctttctcaa aatcacaaat gagtcttttc ctcggttagt
    14161 gattacaaga tatattaaga aaaatgatgg attgtacttt gggccctacc cagattctta
    14221 tactgctaat gaagttaaaa agttattaga taggattttt ccatttaaaa agtgtaaaaa
    14281 tcctatcaat aaggtctgtt tttattacca tttaggccag tgctgtgctc ataccatttg
    14341 tcatacagac aaggcctatt gggatcgttt aattgatgat gttaagcact ttttaaatgg
    14401 taaagatgac aaaattattg aagatcttcg ttctaagatg ttagcagctt ctgaagaaat
    14461 ggcatttgaa cgtgccgctg aatatcgaga tttgatttca ggtattgcca ccatgcgcac
    14521 caagcagcgg gtgatgagca aggatttgca agatagagat atctttggct attatgttga
    14581 taagggatgg atgtgtgtcc aagttttctt tgtgcgtcaa gggaaattaa ttcagcgtga
    14641 tgttaacctt tttccctatt ataacgatgc agaagaagat ttcttaactt atatggggca
    14701 attttaccaa gataaacagc atttcatccc taaagaagtc tttattccag aagctattga
    14761 tgaagaactg gtagccgcta ttgtgccaac caaaatcatc aaacccaaac gcggtgaaaa
    14821 aaaacaactg gtagctttgg ctactaagaa tgctcgtgtt agtctgcaac aaaaatttga
    14881 tttattagaa aaagatatca aaaaaacgag tggtgctatc gaaaatttag gtcagttact
    14941 taggattgac aagcctgtgc gcattgaagc ctttgataat tctaacattc aagggacaag
    15001 tccagttgcg gctatggttg tttttgttga tggcaaacct agtaagaaag attatcgtaa
    15061 atttaagatt aaaacagtgg tgggaccaga tgattatgcc agtatgagag aagtgctttt
    15121 tcgaaggtac agtcgggtga aaaaggaggg actgcaagct cctaacttga ttattgttga
    15181 tggaggcgtt ggacaagtca atgtggccaa agatgtgata gaaaaacaac ttggactcac
    15241 tattcctgtt gcgggacttc aaaaaaacga taaacaccag acccatgatt tgctttttgg
    15301 aaacccgctt gaagttgtac cgttgccgcg ccgttctgaa gagtttttct tactgcatcg
    15361 gattcaagac gaagtgcatc gctttgcagt tacatttcat agacaagtgc gacgtaaaaa
    15421 ttctttttca tcaacattag atcatatttc agggcttggc ccaaagcgaa aacagttatt
    15481 actaagacac tttaaaacca taacagcgat tgcatcagct acctctgagg agattcaagc
    15541 tttaggtata cctaaaacag tagttgaagc aatacagcaa cagataacag acaataaaaa
    15601 tgatagatcg tctccttaaa taagtgttat gttttcagta ctgatgaggt aaaaaggcgc
    15661 tacttgttac gcacgacatt tcagttcgtt attttgaaac tttcagaaag ttgcgaagtg
    15721 tagtgatttt atgattttaa aaactatttt atggtaaaat aaaatggtat taaaaaaaca
    15781 gatttagata ggaagttttg aaatgaaatt tctagagtta aacaaaaaac gccatgccat
    15841 taagaccttt aatgaccagc cagttgacta cgaggatttg cgaacagcca ttgagattgc
    15901 aaccttagca cctagtgcca ataacattca accttggaaa tttgtagtcg ttcaagagaa
    15961 aaaagctgag ttagcaaaag ggttacctct agcaaataaa gtgcaggttg aacaggctca
    16021 atatgtagtg gcgctatttt cagacactga tttagcttta cgttctcgta aaattgcccg
    16081 tatcggtgtc aaatcgttac cagacgactt aattggttat tatatggaga ctttgccacc
    16141 acgttttgct gcttttaatg aggttcaaac cggtgagtac ttagctatta atgcaggcat
    16201 tgtggctatg aacttggtgt tatccttgac ggatcaaaag attgcttcca atattatcct
    16261 tggttttgat aagtcaacga ccaatgaaat tttagacatc gatccgcgtt ttcgtccaga
    16321 attattgatt actgttggct atagtgacga gaaaccagaa ccaagttatc gtttgcctgt
    16381 tgatgaagtc atcgaaataa gatagagtca ggacaattag ttaaatgtaa tgtccagatg
    16441 cgctactgag cgaccactat tgttagactg atactatttt ggcttatttt aagttaaaca
    16501 ttatccctaa aacgaataag gagagtataa tgacaaccat tgattttaaa gcagaagttg
    16561 acaaacgtaa agaagctatg ctagcagatt tagttgacct attacgcatt aattcagaac
    16621 gcgatgacag attggccgat gatcagcatc cctttggccc aggtccagtt aaggctttgg
    16681 agcacttcct cgcaatggct gagcgtgatg gctacaaaac gcgtaatatt gataactatg
    16741 caggtgactt tgaatttggt caaggtgatg aagtactagg aatctttggt caccttgatg
    16801 ttgtgccagc tggtagcggt tgggatacag acccttatga gccagtgatt aaagacgatc
    16861 gcatctatgc gcgtgggtct tcggatgata agggaccaac catggcgtgc tactacgctc
    16921 tgaaaatcat taaagaattg ggtcttcctg tgtctaaaaa agttcgtttc attgttggta
    16981 ccgatgaaga atctggctgg ggggatatgg attattactt tgctcataat ggcttgaaaa
    17041 atccagattt tggtttctca ccagatgctg aatttccaat tatcaatggt gagaaaggaa
    17101 atatcaccga atacctccat ttcgctggtg acaacaaggg cgcttttgtg cttcatcgct
    17161 tccaaggtgg gttgcgtgaa aacatggtac cagaatctgc aacagctgtc attacggcac
    17221 cgcatgacct tgatgtgcta gaagcagcct tggagcaatt tttatcagaa catggtgtta
    17281 aaggttctat gaaggctact gatggtaaaa ttgaagtgac cattattggt aagtctgctc
    17341 acgggtcgac tccagaagcg ggtgttaatg gtgccacctt gttggctaaa ttcctgaatc
    17401 aatttacctt tgaaggggct gctaaggatt atttacatgt ggcaggtgag gtgcttcatg
    17461 aagactttgc ggcagaaaaa ctaggccttg cttataccga tgaccgtatg ggagccctga
    17521 gcatgaacgc aggtgtcttt acctttgata gtcaatcagc tgacaatacc atcgccttga
    17581 atttccgtta tccaaaaggg actgatgcgg caaccttaaa atcaggattg gaaaaattac
    17641 caggtttaac taaggtaagc ctttctgagc atgaacatac acctcattat gtgccaatgg
    17701 atgatgaatt agtaactacc ttacttgctg tttacgaaaa acaaacaggt cttaaaggct
    17761 acgaacaagt tatcggcgga ggaacctttg gtcgtttgct agaacgtggt gtggcctttg
    17821 gagcgatgtt cccaggcgat gaaaacacta tgcaccaagc taatgaatat atgcctttag
    17881 aaaatattta tcgctcagca gctatttatg ctgaagcaat ctacgaatta attaaataag
    17941 atgcttatga cagcaaaaag agctggaaca ctgttccagc tcttttgtga tgattagaga
    18001 atggtcttat ttaccaagac aaaattggct aaagagttgg gtaatgagtt catcaggagc
    18061 agcatcccct gtgatttctc cgagaatttc ccaagttctg gttaaatcaa cctgtagtaa
    18121 atcaactggc atgccaagcg ccaagccgtc attgacagct tctaaactct ggactgcctt
    18181 ttcaatcaaa gaaatgtggc gggcatttga caggtaagtg gcatcttgtt ctactagtcc
    18241 agcattatca aagaagagct ggttgatacg atcttcgatc agattaatgt tctggttggt
    18301 taacaccgaa atagaaataa ggtccgctgg caactgttcc aattcaatct tttgttccaa
    18361 atctgtttta ttcagcaata taatgcggtt actgtcttga ctgagattta acaaagcccg
    18421 gtcttggtca gtcagttttt cagaagcatt cagcacaaga agaacaaggt cagcttcttg
    18481 aagagctttt ttagaccgtt caactccgat ttgttcgacc agatcatctg tctcacgaat
    18541 accggccgta tcgacaagtt ttagaggaac tcccttgata ttaacgtatt cttcaatgac
    18601 atctcgggtg gttcctgcaa tatcagtcac aatagccttg tcttctcgca acaaattatt
    18661 aagtaagctt gacttaccga cgtttggacg accaataata gctgttgata agccctcacg
    18721 cagaatttta ccgcgtttag ctgttcgaag gagattttcc aaaaggcttt ggaattcttg
    18781 tgttttctca cgtagcagag cagtggtcat ttcttccaca tcgtcgtatt cagggtagtc
    18841 aatattgact tcgacttgag ctaaggtgtt gagaatttct tgtcgggtgt cattgataag
    18901 ctgggaaagg gagccatcca gttgcttgac tgcaatcgtc atagccttgt ctgttttggc
    18961 acgaataatg tccatgacag cctcagcttg agtcaaatca acacgaccat ttaagaaggc
    19021 acgtttggtg aattcgccag gctctgccat tctagcgcct tggcgaatca atagttgcaa
    19081 gatttcattt gtcacagcaa taccaccgtg cgtattgatt tcaaccacat tttcacgggt
    19141 aaaggttttg ggagccagca tgactgacac cataacctca tcgacaatag ttcctgtgtt
    19201 aggatcaatg atatggccat agttaatggt gtgtgaagct acttgctcta agttctttcc
    19261 cttaaaaaca gattgtgcaa tggcaagcgc atctgtcccg gatagacgaa caatgccaat
    19321 agctccttca ccaagagggg ttgaaatggc ggtaattgta tcaaattctt tagttatact
    19381 catgccctct attttaaatc aagtcctagt agaaagcaag gtattatttt gtatagaaaa
    19441 taggactagc accattttga gtttagctaa tactaaaaaa gaataaatgt aaaaaaatca
    19501 tttttttaat aatttttatt gacaatagta ttataaaaga atataatgat ataggttttg
    19561 ataaaaaact gacctaagac agcaggggag catgctcata atattcctgc cgaggcacaa
    19621 aacgtgatta aagagaataa cgtatttgta ctttcgtgtc taggtttagg tgcgattttt
    19681 ttgtacctag cccaaaaata aaaacggagg taaaactaat gagtgaagca attattgcta
    19741 aaaaagctga acaagtcgaa cttattgctg agaaaatgaa agcagcagca agtatcgttg
    19801 ttgttgattc acgtggtctt acagttgatc aagatactgt tcttcgtcgt tcacttcgtg
    19861 aaagtggcgt tgagttcaaa gttatcaaaa actcaatttt gactcgtgca gctgaaaaag
    19921 caggtcttga tgagttgaaa gatgtattcg taggaccatc tgcagtagca ttttctaacg
    19981 aagatgttat cgcaccagct aaagttatca acgactttac taaaactgct gacgcacttg
    20041 aaatcaaagg tggtgcaatc gaaggcgctg tttcttcaaa agaagaaatc caagcacttg
    20101 caacattgcc aaaccgcgaa ggaatgcttt ctatgcttct ttctgtactt caagctccag
    20161 tccgcaacgt tgcatacgct gtcaaagctg ttgcagaaaa caaagaaggc gctgcttaag
    20221 acgtctcaga gtagcttaag gctacgctta acattttaaa ttaattattt ggaggaaatc
    20281 acaatggcat tgaacattga aaacattatt gctgaaatta aagaagcttc aatccttgag
    20341 cttaacgatc ttgtaaaagc tatcgaagaa gaatttggtg taactgcagc tgctcctgta
    20401 gctgctgcag ctgctggtgg tgctgaagaa gctgctaaag attcattcga cgttgaattg
    20461 acatctgctg gcgacaaaaa agttggcgtt atcaaagctg ttcgtgaaat cacaggtctt
    20521 ggtcttaaag aagctaaagg tcttgttgat ggagcacctg ctaacgttaa agaaggcgtt
    20581 gctgctgcag aagctgaaga aatcaaagct aaacttgaag aagctggagc aacaatcact
    20641 cttaaataag aagcagatag caatttgaga gcgaaatccc gatttatcag ttttttagag
    20701 cttatagcga tttgaagaaa ctgataaaat gatttggttt cttgccaaaa atcctgccaa
    20761 aaattttttg gcaggatttt tattttctat aaaaattaag ctgagtag
//
LOCUS       DAUELZ010000021        20607 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.021, whole genome shotgun sequence.
ACCESSION   DAUELZ010000021 DAUELZ010000000
VERSION     DAUELZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20607
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.021"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(55..1089)
                     /locus_tag="VHI09_000608"
     CDS             complement(55..1089)
                     /locus_tag="VHI09_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ6120504.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHIFWKELASLVDQAFPGKTLSQAGDLEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEIVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRTPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            complement(1145..2392)
                     /locus_tag="VHI09_000609"
     CDS             complement(1145..2392)
                     /locus_tag="VHI09_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEQ6120505.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDSELLKKADFAVL
                     SSGTTGKAKLFWRCLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINQEVLLNKIPKQWLS"
     gene            complement(2410..3498)
                     /locus_tag="VHI09_000610"
     CDS             complement(2410..3498)
                     /locus_tag="VHI09_000610"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEQ6120506.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNAVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAAFALGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGPYKVAQFSPDTNSPFAMVEGAERVAREHGFT
                     KEYLNHWTLKSHQKASYCQEQTLLVDLILDLPGASDQGIRPRLSSKVLSKVPPILGEG
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGRYGIAAIAAAGGQGFAVLLK"
     gene            complement(3467..4036)
                     /locus_tag="VHI09_000611"
     CDS             complement(3467..4036)
                     /locus_tag="VHI09_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120507.1"
                     /translation="MRRLLSTMIKCNQDLPKNSLVVDMTLEKAVQLYENQVLTDTQRF
                     RKIRLSESGLTLEVRFLKKQPVQWLSESTNSNQLEEKANKFLANDDQTINISQAALAH
                     FLEDTGDQKPIHREVPYVLPGAFLLEQVSDKLAIDYRQLHLRFYAPVVLDSSIQLMMK
                     GRAIGLFQAGKLVAKGDYDHDGCLYCCRP"
     gene            complement(4171..4881)
                     /locus_tag="VHI09_000612"
     CDS             complement(4171..4881)
                     /locus_tag="VHI09_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ6120508.1"
                     /translation="MNFIGELLGTFILVLLGDGVVSACILNKTKAQNSGWIAIVLGWG
                     IAVTVAVYISGFMSGAHLNPAVTLAMAAIGSLPWSQVVTYLVAQFLGAMLGALVLYLH
                     YYPHWKETKDAGTILACFSTGPAIRHAWSNLLGEALGTAVLVITVMAIGPNKVAAGFG
                     PIIVGFVVMAVGFSLGATTGYAINPARDLGPRIMHALLPIPNKGDSDWSYAWIPVLGP
                     ILGGVVGALIYQVILNMM"
     gene            complement(4899..5273)
                     /locus_tag="VHI09_000613"
     CDS             complement(4899..5273)
                     /locus_tag="VHI09_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEQ6120509.1"
                     /translation="MSNLGIIIVSHSKNIAQGVVDLISEVATDVAITYVGGTEDGGIG
                     TSFDQVQAIVDANPKDDLLAFFDLGSARMNIEMVSDFSDKIITLNVVPIVEGAYTAAA
                     LIQAGADKDAILSQLEELAINK"
     gene            complement(5273..5848)
                     /gene="dhaL"
                     /locus_tag="VHI09_000614"
     CDS             complement(5273..5848)
                     /gene="dhaL"
                     /locus_tag="VHI09_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEQ6120510.1"
                     /translation="MQAQDAIKWMTLFNDKIQVNKDYLSQLDTPIGDGDHGGNMARGM
                     SAVMENLDGKEFASAADVFKVVAMQLLGKVGGASGPLYGSAFMGLSKASEDSSLADAL
                     QAGLDMIQKRGKAEKGEKTMVDVWIPVIEALKNDGLTKDVVEEAVLATKDIQATKGRA
                     SYVGERSVGHIDPGSFSSGLLFEAMLEAEVV"
     gene            complement(5859..6848)
                     /gene="dhaK"
                     /locus_tag="VHI09_000615"
     CDS             complement(5859..6848)
                     /gene="dhaK"
                     /locus_tag="VHI09_000615"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEQ6120511.1"
                     /translation="MKKIMNDATQIVDDMLQGLAYMHDDLVERLDGYDVIVRKAEKTG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAICGAVFTSPTPDQILEAIKAADEGAGVFMV
                     IKNYSGDIMNFEMAQELAEMEGIDVASVVVDDDIAVENSLYTQGRRGVAGTILVHKIL
                     GHAAREGKSLADIKALADELVLNIKTIGLALSGATVPEVGKPGFVLEDDEFEYGVGIH
                     GEPGYKKEKLQSSSVLAQELVTKLADSFDMSAGQHYGVLVNGLGATPLMEQYVFANDV
                     AKLLAEKDVTVSFKKIGDYMTSIDMAGLSLTLIKLDKDEWTEALQSDVITPAW"
     gene            7000..7536
                     /gene="dhaS"
                     /locus_tag="VHI09_000616"
     CDS             7000..7536
                     /gene="dhaS"
                     /locus_tag="VHI09_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEQ6120512.1"
                     /translation="MATSLITKKKIAKAFKKQLAVKSFDKISVVDIMDQAQIRRQTFY
                     NHFLDKYELLDWIFETELQEQVTHNLNYISGFQLLDELLFYFERNKTFYAQLFDIKGQ
                     NDFYSYFVDYCQVLMAKIISEYQSQEMSQSDAADYLAFLTHYHARALADMIKGYVSQS
                     EPKPCLTYLKQLILTSIH"
     gene            7551..8540
                     /gene="dhaQ"
                     /locus_tag="VHI09_000617"
     CDS             7551..8540
                     /gene="dhaQ"
                     /locus_tag="VHI09_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEQ6120513.1"
                     /translation="MVTILNQTQTLISQYIASVLAAHPHLAQLQDLPIIYNRHLDDQL
                     IPILSGGGSGHEPAHSGFVGDGMLSAAIYGDIFTPPTATDILEALRFLDKGKGVFVII
                     KNFEADIREFSQAIHLARQEGRNIKYIISHDDISVEPKKQFQMRHRGLAGTILLHKIL
                     GAAAQSGASLDDLEHLAFDLATEIATIGFATKSASLPNAALPLFDLPDHQISYGIGIH
                     GEEGYRTVPFMSSEHLAVEIVNKLQIKFHWQEDEHFILLVNNLGTATDLEQGIFLNDI
                     HQLLDLEGLHLPFIKNGKFMTSLDMTGISVTLCRLKDRQWLDYLQAPTNAYAW"
     gene            complement(8644..9429)
                     /locus_tag="VHI09_000618"
     CDS             complement(8644..9429)
                     /locus_tag="VHI09_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6120514.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFVIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLGAYESAGS"
     gene            complement(9431..10249)
                     /locus_tag="VHI09_000619"
     CDS             complement(9431..10249)
                     /locus_tag="VHI09_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEQ6120515.1"
                     /translation="MQSFWKRYKPFISAGIQELMTYRVNFFLYRLGDVMGAFVAFYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTALLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLISVGFPFLVVIFGIKLMAGMSFWQLLATVFLYLLSLLLA
                     FLINFYFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKVVSIVLSFLPF
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            complement(10251..11243)
                     /locus_tag="VHI09_000620"
     CDS             complement(10251..11243)
                     /locus_tag="VHI09_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ6120516.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYAVLKEIYDVPEKDFRKRMDFLNEVLELNEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQTFGKMKSLSFELEAGQEEVVSQFLGLPDIT
                     VERHELTLEIQYDSSRYQTADVIQRTMADFVVRDLKMTDADIEDIIRRFYRKEL"
     gene            complement(11384..13327)
                     /gene="thrS"
                     /locus_tag="VHI09_000621"
     CDS             complement(11384..13327)
                     /gene="thrS"
                     /locus_tag="VHI09_000621"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEQ6120517.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFRRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSRPDAQA"
     gene            complement(13749..15083)
                     /locus_tag="VHI09_000622"
     CDS             complement(13749..15083)
                     /locus_tag="VHI09_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ6120518.1"
                     /translation="MRVGLFTDTYFPQVSGVATSIRTLKEELEKEGHEIYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGDGPYLQDLKHLAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLS
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            complement(15085..16083)
                     /locus_tag="VHI09_000623"
     CDS             complement(15085..16083)
                     /locus_tag="VHI09_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ6120519.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADPKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            complement(16214..17215)
                     /gene="ccpA"
                     /locus_tag="VHI09_000624"
     CDS             complement(16214..17215)
                     /gene="ccpA"
                     /locus_tag="VHI09_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEQ6120520.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            17389..18474
                     /locus_tag="VHI09_000625"
     CDS             17389..18474
                     /locus_tag="VHI09_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ6120521.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYAEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLVKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKPGVTAAQVDAAARQ
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTYTPKELLYFEG"
     gene            18523..19188
                     /locus_tag="VHI09_000626"
     CDS             18523..19188
                     /locus_tag="VHI09_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ6120522.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            19199..19576
                     /locus_tag="VHI09_000627"
     CDS             19199..19576
                     /locus_tag="VHI09_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ6120523.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            19721..>20607
                     /locus_tag="VHI09_000628"
     CDS             19721..>20607
                     /locus_tag="VHI09_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ6120524.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMSNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPVMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLET"
BASE COUNT         6288 a         4494 c         3765 g         6060 t
ORIGIN      
        1 ccaaaaattc agcctaattg acaatgacga ctaccgttac aaatagagta ctttctaaaa
       61 ccaacccttt ataggatgaa tttggcgttt gaattgctta gcagattttt tgactctggc
      121 aaaactactc ttgttgtggt aagcaaaaag tcctttggca ttaaaataag agaacataaa
      181 gtcatctttt tgagctctaa accactgacg aaatactctt tttggcgttc gaaatccttt
      241 aagggtgtct cttctaaact gaggatcgtg acggctaatc ggatcaacat ctaaatccca
      301 atgtctagga ccatctgtgc catagttaaa agatgcacca ttgacaattc ctttttcggt
      361 cactatttct gcatcaactt cattaagaaa aaagcctgtg ctatctagaa taaattcggt
      421 atgaaaccaa agaagtactt ttatattata ggaaacttct tgacttggaa aaatgacttt
      481 attatcttca aatcttagtt tattatgtaa cctggcagaa tccatcaagg tataatctgg
      541 tgcttgccgc cacaaacaag gccctttttt agacctcaaa aaaagggcca gagcttgggc
      601 gtctgtcatc tcaatcattt tataatgatt tctgatagat tgagcttgtt gactagagat
      661 gacataacga aattgatgca aacgcttttc aagatcacct gcttggctta gtgttttgcc
      721 aggaaaagct tgatcaacta agctagccaa ctctttccaa aatatatgat ctggcaatag
      781 agatggatgg aatcgggtga gtagcgtttg agtttcatgc aactgaccac ttagctcaaa
      841 tggcatctct accaaatcaa gaagaaacgt tagtaactgc ttagctgatt ggttttcttt
      901 ttcaataggc catattaccc agaaagttgg agaaccaagt cgattatcaa tgataggctc
      961 tttagctata gcacgaaatg cttggtttga taaacctgca ttttgatagc atttcaaggc
     1021 ctgatagcga tctaagatag cttgtttcat ggccttctcc tcatcttttt tgaccatatt
     1081 ataacacatc taaagctgaa tcgtcacttg tatctcacta taagcaaaac gaaacctgaa
     1141 atcgttaact aagccactgt ttgggtattt tatttaaaag aacttcttga ttaattttac
     1201 cattatcttt taaaggcaaa caatcaatca caagatagta cttaggaagt tgtccagagt
     1261 ttaaataaag agctaaaaag tctttaatag gtgctaggca atccttctta gtcaaaacta
     1321 atactaatat tagggtttcg ttttctcctt ttcctatctt taaagcgtgt gcttctttga
     1381 cgtttggcgc ttggtgagcg agttcaacta ggctaggcag gtggcatttc actcctcgtt
     1441 gattaaccca gtcatcttgt cgcccttcta aaattaaacc cgctgggctc atttttccta
     1501 agtctgagac actgtatggt tgtgacattc cttctacact ataaggagtc tctacaaaaa
     1561 tttccttatc tttgaaactg atagacacat caggaaaggg ctgtcctacc aaaccattaa
     1621 ttttgacagc agctctgcca ttacaccacg ttataaaaga taattcacta gccccgtaga
     1681 aaatgacaat ttcaagctga ggaaattttt tgtaataatg tcttagtaat tcttgtgata
     1741 tcatttgcga agaggtcaat aagtgagtcg cagtcatgtt gttcttagtc aaataaggta
     1801 gcaagcgatt taggtaagtc ggtaaaaggt aaaggtgact cacctttttt gcttgccata
     1861 agcttaacca cgttttcaaa gacagctttt ggcttaaaac cagacatccc ccggcccata
     1921 gttgagctaa tgctaagtta agattgccag taaaactaaa actaccatgt aagaatagac
     1981 aactattacc agtcatacca aacaccttat tttgataatc aaaaagtctc gtccaagttg
     2041 atagacaacg ccaaaataat tttgctttgc cagttgttcc cgaactcaaa acagcaaaat
     2101 cagctttttt aagaagctca ctatcgacgt gttttatttg ctgctgaaat gtacctgaga
     2161 tattagggtg taaaatgata ggacaactgc cttcttttaa tcctcgtaaa aaagagagta
     2221 actgattaag atagcgacta tgactgatga tatatggtac actgtctttt attgtctgat
     2281 cctttattag aaccgcttgc catagctctt gataagtcaa gcttatctga tcggctacga
     2341 tagctttttt atttgggcat tgctttgccc aatattctag ctttgtcagc atgactaaac
     2401 tccttatgat tattttagta aaacagcaaa cccttggcca ccagctgctg ctatagcagc
     2461 tattccatag cgtccatttt tgatctctaa agccctcatt aagtgcaata ttataatggc
     2521 tgctgaggca ccgtagggat gaccataagc taatgcacca ccaaatatat tatagcggtc
     2581 caataaatct ggataatggg tttcaaatag accatcaata acggcaaagg cttcgttcca
     2641 ttcaatcgct gtcatatctg ccatccctag accatgtttt tccaaaagga cttgactagc
     2701 cttaatcacc attaaagggc tacgctgtgg gtctcctgct acttctacca catcaatcag
     2761 tttaaaagct gaaggctgac tacttagctg taaaaaggca gcagcatcat gggtgaggca
     2821 ggcattggct gcactaatca cgtgaccttc tcctaagata gggggtacct ttgacaacac
     2881 cttacttgac aagcgcggtc gaatgccttg atcactggcc cctggcaggt ctaatataag
     2941 gtcaactaac aaggtttgct cctggcaata actagccttt tgatgactct tcaatgtcca
     3001 gtgattcaaa tactcctttg taaagccatg ttctcttgcc actcgttctg ccccctcaac
     3061 catcgcaaat ggactattgg tgtccggaga aaactgggca actttgtaag ggccttggcg
     3121 ccaatcagct gaagcatata ccgattcagg ttgtaaggag ctactttcaa tccctcccac
     3181 taaaaggtta cttgccatac ctgccttgat tttagcataa cctagagcaa aggctgcacc
     3241 agctgatgca cactgcatat caaccgtcaa agcagagaca gattctccta aatgagaata
     3301 cagggtcatc aagcgcccaa tattaccacc cgttccgacg gcattgccac aaatcacttg
     3361 gtcaatggga actggatact tattttgtaa agcattgatg agctttgctc caaggatttc
     3421 tggttgttct ttagcaaatt gtttccctac taaaccgata ggggttctaa ggcctgcagc
     3481 aatataaaca tccgtcatgg tcataatctc ctttagctac taatttacca gcctgaaaca
     3541 ggccaatggc tctacctttc atcatcaatt ggatagagct atctaaaacc actggcgcat
     3601 aaaagcgcaa atgtaattga cgataatcga tagctaactt atcactcacc tgctcgagta
     3661 agaatgctcc gggtaaaaca taaggcactt ctcggtgaat aggtttttga tccccggtat
     3721 cctctagaaa gtgagcgagg gcagcttgac tgatatttat tgtctgatca tcatttgcta
     3781 aaaacttatt agccttttct tctaattgat tactattggt ggattctgat aaccactgaa
     3841 ccggctgctt cttcaagaat cggacctcaa gcgtcagccc tgattcactc aaccttattt
     3901 tccggaaacg ttgggtatcc gtgagcactt gattttcata taattgcaca gctttttcta
     3961 aggtcatgtc aaccaccagg ctgttctttg gtaaatcttg gttgcactta atcatggtac
     4021 ttagtagtct tctcattgtc accctcattt ttcaatcacg ttaactttat tatactgaaa
     4081 gctccggtcc aagaaaaggg aaaatctgta aaagaacact tcttagccac caaaaaaagt
     4141 tgagtcctag acccaacttt ttttggttca ttacatcata tttaaaatca cttgataaat
     4201 caaggcacct acgaccccac caaggattgg acctaaaact ggaatccaag cgtatgacca
     4261 gtcagaatct cctttatttg ggattggaag taaggcatgc atgatacgtg gcccaaggtc
     4321 acgcgctggg ttgatggcat aaccagtggt tgctccaagt gaaaaaccaa ctgccataac
     4381 cacaaaacca acaataatcg gaccaaaacc agcagctact ttattagggc caatggccat
     4441 taccgtaatc actaaaacag ctgttcctaa agcttcgccc aaaaggtttg accaagcgtg
     4501 gcgaatagct ggcccagttg agaaacaagc taaaatcgta ccagcatctt tggtttcctt
     4561 ccaatgtggg tagtaatgca agtagagaac taaggctcca agcattgctc caagaaattg
     4621 agccacaaga taagtcacta cctgagacca tggcaatgag ccaatggctg ccatagcgag
     4681 tgtcacggct gggtttaaat gggcaccact catgaaacca ctaatataaa ccgcaacggt
     4741 tacagcaatt ccccagccca aaacaatagc aatccagcca ctattttgag ctttggtttt
     4801 attcaagata caagcagaaa ccacaccgtc tcctaataaa accaaaataa acgttcctaa
     4861 cagttcaccg ataaaattca tgataatctc cttgctattt atttattgat agctaactct
     4921 tctaactgac ttaaaatagc atccttgtcc gcgccagcct gaataagagc tgctgctgtg
     4981 taagcccctt cgacaatggg aaccacattc aaggtaatga tcttgtcaga aaaatctgaa
     5041 accatttcga tattcatgcg ggcagatcca agatcaaaga aagccaataa gtcatcctta
     5101 ggattggcgt caacgatagc ctgcacttgg tcaaaactgg tgccgatacc accatcttct
     5161 gtaccaccga cataggtgat ggcaacatct gtcgccacct ctgaaatcag gtcgacaact
     5221 ccttgagcaa tatttttaga atgcgataca ataataattc ctaaatttga cattaaacca
     5281 cctctgcttc caacatggct tcaaataaga gtcctgatga gaaggaacct ggatcaatat
     5341 gacccactga gcgttctcct acataagaag cgcgtccttt agttgcctgg atgtctttgg
     5401 tagctaaaac agcctcttca accacatcct tggttaaccc gtcatttttc aaggcttcaa
     5461 tcacaggaat ccaaacatcc accatggttt tctcaccttt ttctgcctta ccacgttttt
     5521 gaatcatgtc caatccagcc tgcaaagcat ctgccaaact gctatcctca ctagcttttg
     5581 aaagccccat aaaggcagaa ccatacaatg ggcctgaagc tccaccaact ttgcctagca
     5641 actgcatagc aacgacctta aagacatccg ctgcgctcgc aaattcttta ccgtcaagat
     5701 tttccatcac agcagacatc ccgcgcgcca tattgccacc gtggtcacca tctccgatag
     5761 gcgtatccaa ttgacttaaa taatccttat tcacttgaat tttatcatta aataaggtca
     5821 tccatttgat tgcatcttgt gcttgcatga tttcctcctt accaagctgg tgtgataaca
     5881 tctgattgaa gagcttctgt ccattcatcc ttgtcaagtt tgatcaaggt caaagacaaa
     5941 ccagccatat caatagacgt catgtagtca ccaattttct tgaagctgac tgtgacatct
     6001 ttttctgcta agagttttgc cacatcattc gcaaaaacgt attgttccat taatggtgta
     6061 gctcccaaac cattgaccaa gacgccataa tgttgtcctg cgctcatgtc aaagctatct
     6121 gccaatttag tcaccaactc ttgagctaat actgatgatg attgtaattt ttcttttttg
     6181 tagcctggtt caccgtgaat accgacaccg tattcaaact catcatcttc aagcacaaaa
     6241 ccaggtttgc caacttcagg aacagttgct ccagaaagag ctaaaccgat agttttaata
     6301 tttaggacca attcatcagc aagagcttta atatctgcta atgatttgcc ctcacgcgca
     6361 gcatgtccca aaatcttgtg aacaagaatg gttcccgcaa ccccacggcg tccttgagtg
     6421 tagaggctat tctcaaccgc aatatcatca tcaacgacaa cactggcaac atcaatacct
     6481 tccatttctg ctaattcttg ggccatttca aagttcataa tgtcacctga ataatttttg
     6541 atgaccataa agacaccagc accttcatca gctgctttga tagcttctaa aatttgatct
     6601 ggagttgggg aagtaaagac tgcaccgcag atagctgctg acaacatgcc ttcaccaaca
     6661 aaaccagcat gagatggctc atgtccagaa ccaccacctg aaataagggc tacttttccc
     6721 gtcttctctg ctttacgaac aatcacatca taaccatcta ggcgttccac taagtcatca
     6781 tgcatataag ctaaaccttg caacatatcg tccacaatct gagtggcatc attcataatt
     6841 tttttcatgg atcttcctcc gattttttgt ttacgctttc attataaaat agcccctccc
     6901 aaaacgtaag ggacaaatcc tcagatttgt cccttttctt tctaaaatgc ctttgataag
     6961 gtataatggc agaagatatt tcatattaaa ggattactta tggcaacttc tctcatcacc
     7021 aagaaaaaaa ttgccaaagc ttttaaaaag caactagcag tgaaaagctt tgataaaatt
     7081 tctgtcgttg atattatgga tcaggcccaa atcaggcgcc aaaccttcta caatcatttt
     7141 ctagataagt atgaactgtt agactggatt tttgagactg aacttcagga acaggtcaca
     7201 cacaatctca attacatcag tggctttcaa ctgttagacg agttgctctt ttattttgaa
     7261 cgcaacaaaa cgttttatgc ccaactattt gatatcaaag ggcaaaatga tttttacagc
     7321 tactttgtgg actactgtca ggtcttaatg gctaaaatca tttctgaata ccaaagtcaa
     7381 gaaatgagcc aatctgatgc tgctgattat cttgcttttt taacccatta tcacgctagg
     7441 gcattagcag atatgattaa aggttatgtt agtcagagtg agcctaaacc ttgtcttacc
     7501 tatctcaagc agcttatctt aaccagcatt cattaaacga ggaaaacgtc atggtaacca
     7561 tattaaatca aacacaaaca cttatttccc aatatatagc aagtgtatta gcagcccatc
     7621 cccacctagc ccaattacag gatttgccta ttatttataa taggcatctc gatgatcaac
     7681 ttatcccaat tttatccgga ggaggatccg gacacgaacc tgctcattcc ggcttcgttg
     7741 gtgatggtat gctgtcagct gctatctatg gcgatatttt tactccccca actgccacag
     7801 acatcttaga agccctgcga tttctagata aaggtaaggg ggtctttgtc attatcaaaa
     7861 actttgaagc tgacattcgt gaatttagcc aagccattca tctagcacga caagaaggtc
     7921 gtaacatcaa atatattatt tcccacgacg atatctcggt tgaacctaaa aagcaattcc
     7981 aaatgcgtca ccgaggactc gctggaacta tattgcttca caaaatctta ggagcagctg
     8041 cacaaagtgg ggcaagtttg gatgaccttg aacacctagc ttttgacctt gccacagaga
     8101 ttgctaccat cggatttgca accaaatctg ctagcctacc caatgcggca ctacctcttt
     8161 ttgacttgcc tgatcatcag atttcatatg ggattggtat tcatggtgaa gaaggctatc
     8221 gcacggtccc atttatgtca tcagagcatc ttgctgtgga aattgttaat aagcttcaaa
     8281 ttaaattcca ttggcaagaa gacgaacatt ttattcttct agtcaataat ttaggaacag
     8341 caaccgactt ggaacaaggc atttttctga atgatatcca tcaactttta gatcttgaag
     8401 ggcttcatct tccttttatc aaaaatggaa aattcatgac tagccttgac atgactggta
     8461 tctctgttac cctttgccgt cttaaagata gacagtggct agactactta caagctccaa
     8521 ccaatgccta cgcttggtaa accagcacaa aaaaggcagc cgtttttaca acagctgcct
     8581 ttcttcatta actaggtttt catattctga cctaaacgat tttcaatttg tttaagagtc
     8641 tctctaggaa ccagcacttt cataagcacc tagtccccat ttccaaagct taagcgacaa
     8701 ggccaaaacc agtaacgaca ccactaccaa gcctccaata ttaaagagca ggtgctgacc
     8761 tgttaaaaaa taagaagcag ggtaataagc tgtaaaagca aagggaatga caaaactgat
     8821 taaccaccgc aaaaaggaat ggtaaatgga cataggatac ttggaaaagt cattaaacat
     8881 gtaaaaaata tagataactg ccccagactg cttggtccaa aaagaaatgc tggcggtagc
     8941 aatttttaaa gaggtgtaaa tcaaggtcgc aaaaggaata accagaataa acaaaagcac
     9001 cttaggaaga gtccatacga tactaccagc cgtggtcact aataagataa ccccaaccaa
     9061 taattcaccc aaggcatcca cttgaaatgt ctcaaccaaa acatgaaaca aaggactaat
     9121 tggtctggtg aggtacttgt caaattctcc tttgcggaca agccgttgcc ctaaagccca
     9181 aagattgtca aaaaagaggt gatcaattcc ctttggaatc aaagaaaagc cataaataaa
     9241 ggcaatttgt tcaaacgtcc agccttctaa agaagggata tgttggaaaa gtaccgataa
     9301 aaagagcaga ttaagtccct gagtgagaaa aacacctagc actcccacca caaaatcaac
     9361 cttgtattcc ataatttgtt tgaggtattg tttgataaaa atagcgtgca tacgtctcaa
     9421 ttttgccatc tcaccctcct tgaatcgtta aatggtactg aacctttttc cagatgactt
     9481 gcgataagac taccatcacc agtaaccaaa aaatttgcaa acttagggcc accattatct
     9541 gactaaggct gtatttccca atgacaatca taactggcgt gtaaaccaaa gaggaaaacg
     9601 gtagaaaact aagcactata gaaaccactt taggaaaaaa agccaatgga atcaaactgc
     9661 ctgacataaa ggcaaccaag gcattcttta ataagttaga accccaaagg tttttgaaaa
     9721 caaaggcaga aaagccaaaa cagatattga aataaaaatt aatcaagaag gccaataaaa
     9781 gactaagcag gtagagaaac acagtagcta gaagttgcca gaacgacatt cccgccatta
     9841 gtttgattcc gaaaatcacc accaaaaacg gaaagccaac actgatgaga acaatccacc
     9901 gaaagcctat ctccataaaa aggtaagaag ccgcaaagtg aactggccga agcaaccgca
     9961 tgataataga cccatctttc acttcctcac caatcataaa ggagctatcc gatttcgtta
    10021 acaaagccgt cacaaaactc atgataatat aaaaggtcat atctgacaga gtaaaaccat
    10081 taatcaagga ctgtttggag gaatcaaaca cagccttcca aagataaaaa gccacaaaag
    10141 ctcccataac atcccctagt cgatagagaa agaaattgac ccgataagtc attaattctt
    10201 gaattcctgc actgataaag ggcttatagc gtttccagaa tgattgcatg tcacagctcc
    10261 tttcggtaaa aacgacggat gatatcttca atatcagcat ctgtcatttt caggtctctc
    10321 accacaaaat cagccatggt tcgttgaata acatctgctg tttggtaacg ggaactgtca
    10381 tattgaattt ccaaagttaa ttcgtgtcgt tccacagtta tatcaggcaa accaagaaac
    10441 tgagaaacaa cttcttcctg tcccgcttcc aattcaaaag ataagctttt catcttccca
    10501 aatgtttgtt tgagttgagt caccgtgcca tcaaagattt cttggccctt atcaatcatg
    10561 ataatccgat cacacagttg ttcaatatca ctcaaatcat gagttgtcaa caaaatggtt
    10621 gtctcttctt cttgattaat ctgagtgatg gcacgtcgaa tattgtcttt gacagaaaca
    10681 tccaagccaa tcgttggttc gtctaaaaaa agcactttcg gattatgcaa taaagaggct
    10741 gcgatgtcag ctcgcatgcg ttgtcctagg gacaacgttc gaacaggatc cttaataaac
    10801 tcgttaagtt ctaaaacctc atttaaaaaa tccatacgct tgcgaaagtc tttttcaggc
    10861 acatcgtaaa tttcttttaa cacagcatag gtttcctgca aggccaaatc ccaccacagt
    10921 tgggtccgtt gcccaaaaac aacgccaata tctttgacat aatcttgacg attatcttgc
    10981 ggaatcttcc cgttaatgcg acaataccca gatgttggct ttaaaattcc tgttaacatc
    11041 ttaattgttg ttgattttcc tgcaccattg gcgccaataa aacctaaaat ttgccctttg
    11101 ggtacctcaa aagataagtc cttaaccgcc tcaaagattt ctctttgggg atgaacaaag
    11161 gacttcaagg ctccctttaa tcctggctct ttaatggttt tggaaaaatt tttttgaagg
    11221 tgggacactt ctatcatgac catttaccat tcctcgtttc tattatcgtc ttatcaccgt
    11281 tactaaacaa tttattataa caccatgaaa aagacggtgc aaccgtttgc ttaatttttt
    11341 aagaaagaaa aagaagccca taaggacctc tttttgccat tgattaagct tgtgcatctg
    11401 gacgtgactt gcgagcaatg tctgccaaga tgttttccac aaattcttcc acagattcgg
    11461 tgtgagtcgc cttgctacca tagcggcgaa cattgactga tttgtcttcc atttctttgt
    11521 ccccaacaat taattggtaa ggaattttgc tggtttgaga cgcacgaatc ttgtactgca
    11581 ttttttcatt acggtcatcg acatctgcac gaacaccgcg gtcacgaagg gtttttgcta
    11641 cttcccaagc atagtcaatg tgggcttcat ttgaaattgg aatcacggtt acttggtgag
    11701 gtgccaacca agttgggaaa gcacccttgt aagtttcaat aaggatagca gtgaaacgtt
    11761 ccatagttga gataacacca cggtgaatca tgactggacg gtgttcttcg ccatctgcac
    11821 caatgtattt aaggtcaaag cgttctggca acaagaagtc caactgaatg gttgataggg
    11881 tttcttcgtt accaagagct gttttcacct gaatatcaag cttaggccca taaaaggctg
    11941 cttcaccttc tgcttcaaag taatctacac ccatctcatc aagtgcagct ttaagcatac
    12001 tttgagcatt ttcccacatc tcatcattgt cataatattt atgagtatcg ttagggtctc
    12061 ggtatgacaa acgaaaacga taatcagtca agttgaagtc agcataaaca tcaatgatta
    12121 attgaagggc acgtcgaaat tcttcctgga tttgctctgg tgttacgaaa atgtggccat
    12181 cattcaaggt catttcacgt acccgttgaa gtcctgatag ggcacctgat ttttcgtaac
    12241 ggtgcatcat gccaagctcg gcaatacgaa taggcaattc acgataagaa cgcacatgat
    12301 ttttatacac ttgaatgtgg tgtgggcagt tcattggacg aagaacaaac tcctcaccat
    12361 ctcccatatc cattacaggg aacatatctt cttgataatg atcccaatga cctgatgtct
    12421 tgtacaattc tacagaagca agtggtggtg tgtaaacgtg ttggtagcct gatgctaatt
    12481 ctttatctgt gatgtagcgc tcaagtgtac gacgaatagt cgctccatct ggtaaccaaa
    12541 acggcaagcc ttgaccaact tcttggctaa tcatgaacaa gtcaagctct ttaccaagct
    12601 tacggtgatc acgttctttg gcttcttcta gacgagtcaa gtaagctttg aggtcttttt
    12661 tatcaaacca agctgttcca tagatacgtt gcatcatgtt attgtcgctg ttaccacgcc
    12721 agtaagcacc cgccacattg agcaagtgga agacttggat acggcctgtt gatggcacat
    12781 gagggccacg gcaaagatcc acaaattcac cttgacggta aaccgtcaag ccagcaccag
    12841 catgctcatt aatcaattct accttgtaag gatcatcttt gaacaattcc aaagcttctt
    12901 ctttggtcac ttcttcacgg atacatgggt aattttctgt cacgattttt tgcatttcag
    12961 cttcgatacg tggcaaatct tcgtttgaaa tttggccttc tgcattatct gtatcatagt
    13021 aaaagccttc tgcaatggca gggcccacac ccaaatgaag atttgggaac aagcgttttg
    13081 cagcttgggc aaataaatgg gctgcagagt gacgaagcac ctcataagca tctttgtgat
    13141 caggtgtcac gatttcaata ctgccatctt cttcaatggc acgagtggtg tcaatgagtt
    13201 gatcattgaa cttaccagct aaagcttttt tagccaatga tttgctgatg gactcagcaa
    13261 tgtcaaacgt tgttacgcca gattcaaatt cacgcacggc gccatctgga aaagtaatct
    13321 taatcatttg ctttctcctt taagtcagtt aagatttcca ctaaaacatg gatagtaaga
    13381 caaattaggg acaagaggtt agtgaatagc tcaacaccac cacgtagaag aacaatacct
    13441 tattcacgaa tctaccctcc ctagtcctta aaaacaaggc taatcccctc caaaacaaaa
    13501 aaactgaccg catcccaaag gacgcaatca gcgtggttcc accttaattt atgcaggaca
    13561 aaaaacatgc cttgcacctc tagtggcgat aagggggcca cccttttatg tttgcataaa
    13621 aaccaggaag gagtatcaga ttagctatcc ttgtgctctt tccaccaacc agcactcgct
    13681 taaaagattc tgtccaatct aacttgtctt caagataatt atagcaagtt ttcaaaaatt
    13741 tacaagcctt aatccagaaa atctttgata gcatggacca gcttgatagg agccttgact
    13801 acttttactg aggtgatagc tgtgacctta gcagcacgtt taggcaagtg aatagcacct
    13861 tgcactaatt ttagagaact acttttacca gaatgattaa gataaagact caacttttga
    13921 gcttctttgc tatttctagc aattaacgta tctaaataga acgtgtaaat agattttcca
    13981 aagtgctgtg ctgagatttc ataacgtttt tttgctaata accgtttatc cataactggt
    14041 gtttttagta tggcatcaat aatagcatca gttaaatctg tttcagcgta ataaagagtg
    14101 ccaaacattt tatcagtcac cacatcatct aaataaggat tgccatgagc aataatagga
    14161 gtgccactag ccaaactttc aatataggtc aagccctgag tctcactggt cgatgctgag
    14221 ataaagaaat cacaagcctt atagtacaga gcaaccttat catgaggcac catgcctgta
    14281 aaggtcacgt gtttgtcaac ctctaactgc atcgccaagt gtttcaaatc ttgcaaatag
    14341 gggccatctc ctacaataat aagctttatc ttggcatttt cagccaaaat agctggcatc
    14401 tgattgatga tagcttgaat atttttttca taagaaatcc gtgacaaact caataacatg
    14461 gtttcatcac cagcaatgcc caattctgct tttaagttgg ttacttcttc tgctgtgatg
    14521 tcatcacgaa tatatttttc caaaggaatg cctgttggga taacccgctt agggatagtc
    14581 acttcgtaac cttctagaag attgaggaca atcctgcttg ggcagataac cccatccaaa
    14641 tccttcaaat agcccctaag aagaggtttg accatacttg gtcgaatgat ttttccgttg
    14701 gcaatataac tcacgtagtc ctcatattgg gtatggtaag tatggacaac aggaattcgc
    14761 aaagctttgc ctatcatttt ccctaataaa cctaagctaa actcagtttg cgtatgaata
    14821 atatcaagat tatagtgttt tgcaattttg tatgacgaaa tgaggccacg ataaaccaca
    14881 cgtctatccg taaatgacac aaaaggaaca cttggcagtc gaataatggt cgggtcttca
    14941 aagcgtttga catctctatc agtagtggtg aaaatataaa tttcgtgacc ttctttttct
    15001 agctcttctt ttaacgtacg aatactagta gcgactcctg aaacttgtgg aaaataggta
    15061 tctgtgaata gacctacacg catcttataa ctccattact tttttataga catctactaa
    15121 ggcatggcca accgtttcta ggcgacgact ctgggcaacc ttgtaaccag cttcaacttt
    15181 gttgctggca ccagaaaaga cttttttcag agcttctata aaacctggta tatcggttgc
    15241 taattccgca ctactttgat caacccatcc gtagtaaact ggtatatcac gtaaaacaag
    15301 gtgctggcga ctggccaagg cttctaagac aacaatgcct tctgtttctt cacgacttgg
    15361 aaagaaaaag gcatctgcac cagtcatggc accttcataa acatcccctt taatgtatcc
    15421 tgggaaaata agatttttcg ggtggttacc attgaccatt tggcgaactt gagcaggaat
    15481 gacccatttg ttggtctcgc caaaccagat aaaacgaaca tctggcatag cttgggcaac
    15541 tttgacaaag tcatcaattc ctttcctcag aaaaaataat cctgctccca taaccacttt
    15601 ttcgccctct ttaatgtcaa agtagcgacg aaaagctgct tcctttttag gatctgctcc
    15661 gtactgctcc aaatcaatcc catttgacac tgcaaaaata ggtgacttca ctccataact
    15721 ctcaatcaaa gacttagaat atagggtagg ggtaatgata gcatctgcct tattgtaaaa
    15781 gtgacaaagg tattttttaa accaaggaga taatagattt gaaaaaataa aagaatttct
    15841 aaaatcttct tctgtagaat gcccatgcat gataaccttc ttaccagctt tttgtgcttt
    15901 tatcatcagc agccaacttt ttaaaccata ggtattgaga tgaaccaaat cataagtttc
    15961 tcttggatta gtcgtaaaat gttgaccaac aagtgacaag gctttagcct gatgcttaat
    16021 cgctcgacca attcctgatt ttcttagata attttctgct tctaaataca ataagacttt
    16081 cataagttct attatagctc attttatagt atttttcact aagacatgca aaaatcagcc
    16141 ctaaggctga ttttagtatt gaagaaacag tctattgtga tactttttgt tagatggtgc
    16201 tcataagtca cagttactta gttgtcccac gttttttaat accatgattc aaaagaattt
    16261 ctttttcttc caactcttct ttgttcatga ttttagtcaa catccgcatg ctaacagcac
    16321 ctaagtcata aacaggttga ctgatagaac tcaaattggg acgagtgtat tgaaccactg
    16381 gtgaatcgtt gctggtgatg atttcaaagt cctctggtac gcgtttacct gcttcaaaca
    16441 agccgtttaa aaggcctgct gccaattcat cttcagccac ataagccgct gttgctcctg
    16501 agttaatgac gcgctgcgcc aattcaaatc cttctttata agaatagttc gcttcaaaaa
    16561 caagaccttc tttgaagtca agcttattgt gcttcaaccc ttctttgtac cctgctaagc
    16621 gcactttacc attgatatca tcaatgagtg gtcctgacac aaaagcgata cacttatgat
    16681 tttcagctaa aatatcaaca acgtttgaca ccgcagctct atagtcaatg ttgacgcttg
    16741 gtaattgatg atcaagatct acagttcctg ccaaaacaac tggagtacga gagcgtgaaa
    16801 attcggcccg gattttttct gtcaagtgat gccccataaa aataatacca tccacttgct
    16861 tggcaaaaag ggtattgaca acattaacct ccttgtcatc atcttcatca ctggaggcaa
    16921 gcacaatatt atatttgtac atagcggcga tatcgtcaat acccttagct aagatagaaa
    16981 aataactatt tgcaatattt ggaatcacaa cgccaacggt tgttgtcttt ttgctggcga
    17041 gaccacgcgc gacagcattg ggacgatagt caaggcgatc aataacttct aaaactttct
    17101 tacgtgtatt ttccttaaca tttttattgc cattaacaac acgactaacg gttgccattg
    17161 aaacaccagc ttcacgggca acatcataaa ttgtaatggt atcatctgta ttcatttttt
    17221 atcttccttt ctatttgaaa atgacgcttt caaaaagtat tttagcatga tttgtaacca
    17281 ctttcaagtg ttataggtta atttttgaaa aagttttcaa aaattaactc gaagcttgat
    17341 tttttaagct ttttttgaaa aaatagtaag tagaaagaag gttagcctat gacaaaatta
    17401 gaccaaatac gtttgtatct tgaccaaaag ggagctgaat tggcgatttt ctcagatcct
    17461 gttacgatta actatttaac tggattcttc tgtgacccac atgaaagaca actgttcttg
    17521 tttgtctacc atgatttagc tcctgtgctt tttgtacctg cccttgaggt ggccagagct
    17581 agccaggcca tatcatttcc tgtgtttggt tatgttgatt ctgaaaatcc ttgggaaaag
    17641 atcaaggccg ttttaccaaa tacagcagcc aaaaccattt atgctgaatt tgaccacctt
    17701 aacgtcaata agttccatgg cttgcagact atcttttcag ggcaatttaa caatctaacc
    17761 ccttatgtcc aaggcatgcg cctcgtcaaa tcagctgatg aaatcaataa aatgatgatt
    17821 gccgggcagt tcgctgataa ggctgttcaa gttggttttg acaatatttc cttagatgct
    17881 accgaaacag acgtcatcgc tcaaattgag tttgaaatga aaaaacaagg cattcataaa
    17941 atgagttttg acactatggt cttaactgga aataatgcag ccaatcccca tggcattcct
    18001 ggaacaaata acattgaaaa caatgccctt cttctctttg acttaggtgt tgagacctta
    18061 ggctacacca gtgatatgac cagaactgtg gctgttggtc agccagacca atttaaaatc
    18121 gatatctaca atctctgcct agaggcacaa ctagcggcta ttgactttat caaaccaggt
    18181 gtaacagctg ctcaagtaga tgctgctgct cgtcaagtga ttgaaaaggc tggatatggt
    18241 gagtacttca accaccgctt gggacatggt atcggcatgg atgtccacga atttccctcc
    18301 atcatggctg gcaatgactt ggtccttgaa gaaggtatgt gcttctctgt ggaaccaggc
    18361 atttacattc caggtaaagt tggtgtgcgc atcgaagact gtggccatgt cactaaaaat
    18421 ggctttgaag tctttaccta tactccaaaa gagttgcttt attttgaagg ctaagcccct
    18481 accatctggt taaacaaacc ctcgcgaaag gaaggtattc gtatggatca aaccattcat
    18541 caccaaatac agcaagcact acactttagg acagccgttc gtgtttataa ggaagaaaag
    18601 atttctgatg aggatttagc ccttatcctt gatgctgctt ggttaagccc ttcttctgtt
    18661 ggcttagaag gctggcgctt tgtcgtttta gacaacaagc ctattaaaga agaaatcaag
    18721 ccctttgcct ggggggccca gtatcaactg gagacagcta gtcactttat tcttttaata
    18781 gccgaaaaac atgcaagata cgatagccct gctatcaaaa atagcctttt acgacgtggt
    18841 atcaaagaag gtgatggtct caacagccgc ctaaaactct atgaatcttt ccaaaaagag
    18901 gacatggata tggcagataa tcctcgggca ctctttgatt ggacagctaa acaaacctat
    18961 atcgctcttg gtaatatgat gatgacagct gctcttttgg gcattgatac ttgccctatt
    19021 gaaggctttc attatgataa ggtcaatcat atcctagcta agcataatgt gattgattta
    19081 gaaaaagaag gcattgctag catgttatcc ctaggctatc gtttgcgaga tcccaaacat
    19141 gctcaagttc gtaagcctaa agaagaagtg atttcagtcg ttaaataagg agattaaaat
    19201 gaaagcatta catacctgta tccgcgtcaa agaccttgac cagtcggtag ctttttacac
    19261 aagcgccttc ccttttaagg aaaactatcg taaagatttt ccagatagtc aatttactct
    19321 tgtttatctg gctttagaag gagaaagtta tgaattagaa ttaacctaca attatggtca
    19381 tggcgactac gacttaggaa atggctatgg tcatattgcc cttggctctg aacattttga
    19441 agcagaccat aaaaaacatc gacaagcagg ttttccagtt accgatatca aagaactggc
    19501 tgacaaatct gcccgctatt atttcattca ggatccagat ggctacaaaa ttgaagtcat
    19561 tgatttaaac aactaatact attaacaaca gaagctttaa agcttctgtt gttttatttt
    19621 ttaacctgac gattgggata atctcattat taacagagtt ttgaaatatc tcagcttttg
    19681 tgttacaatt gccctaaaat tccatttgaa agaaaagtct atgacactac tatctatcat
    19741 tgtcccctgc tttaatgaag aagcaaacat tctcccttat tttgaagaaa tgcatcaact
    19801 agaaacgtcc atgtccaacc aacttgcttt tgaatacatt tttattgatg atggttctaa
    19861 ggataacacc ttaggtattt tgcgtgagct agctgctcgc tttccaaacg tgcattatct
    19921 gtctttttca cgccattttg gcaaggaagc tggactctta gctggactaa aagaagccaa
    19981 aggcaattac attactgtga tggatgtgga tctgcaagat ccacccgaac ttttgccagt
    20041 tatgtacgct aagctaaaag aaggttatga catcgttggc accaggcgcc aaaatagaca
    20101 aggagaacca ctaattcgtt caatgtgttc gaatctattt tatggactga tcaaacactt
    20161 atcagatact gaaatggtca atggagttcg ggattatcgg ttaatgacca gacaagtcgt
    20221 tgatagcatt cttgagttgg gagaggttaa tcgtttttca aaagggatct tttcgtgggt
    20281 tggttatcgt atcacctatc ttagttttga aaatcaaaaa cgcaaatatg gaaaaagtcg
    20341 ttggcatttt tgggagttgc tccgatattc tttagatggc tttatcaact tttcagaaat
    20401 gccgttaacc attgctacct ggacagggac tttcagcttt ttgatttcta ttttcgccat
    20461 cttatttatc attatcagaa agatactctt tggtgatcct gtctctggtt gggccagcac
    20521 tgtctctatt attttattca tgggaggcat tcagctcttt tgcatgggta ttattggaaa
    20581 atatatttca aaaatattcc ttgaaac
//
LOCUS       DAUELZ010000022        20286 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.022, whole genome shotgun sequence.
ACCESSION   DAUELZ010000022 DAUELZ010000000
VERSION     DAUELZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20286
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.022"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(103..1548)
                     /locus_tag="VHI09_000629"
     CDS             complement(103..1548)
                     /locus_tag="VHI09_000629"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEQ6120525.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGLSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMA
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISQEIASHIARPVEIISDREQAIQKAMSLCQEAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIATHYL"
     gene            1636..3270
                     /locus_tag="VHI09_000630"
     CDS             1636..3270
                     /locus_tag="VHI09_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEQ6120526.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSAIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAMSQIAEQLIRVIWMLLTTFFIMKLGSKDYLSAVTQSTLAAFVGMIASMAVLGY
                     YLWKLGLLKAICRKPNHHIDSDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLMALYT
                     LFSPMLQALFENRKAIYYFVYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYAFKPTGRLTSLLYLLII
                     GGVGMTAYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            3438..4067
                     /gene="upp"
                     /locus_tag="VHI09_000631"
     CDS             3438..4067
                     /gene="upp"
                     /locus_tag="VHI09_000631"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEQ6120527.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            4291..4881
                     /locus_tag="VHI09_000632"
     CDS             4291..4881
                     /locus_tag="VHI09_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEQ6120528.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK"
     gene            5376..5669
                     /locus_tag="VHI09_000633"
     CDS             5376..5669
                     /locus_tag="VHI09_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEQ6120529.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIIYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKGYQPPSY"
     gene            5900..6535
                     /locus_tag="VHI09_000634"
     CDS             5900..6535
                     /locus_tag="VHI09_000634"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEQ6120530.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGRLVFIDRFIDS
                     SVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            6553..7428
                     /locus_tag="VHI09_000635"
     CDS             6553..7428
                     /locus_tag="VHI09_000635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEQ6120531.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHTNAANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            7447..7686
                     /locus_tag="VHI09_000636"
     CDS             7447..7686
                     /locus_tag="VHI09_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tpl protein"
                     /protein_id="HEQ6120532.1"
                     /translation="MGKRSLSKDLPVATKVAAHRGKKAFEENTAFATDLMKEVSRLIT
                     QNKLEMTFINSVFLLDQHVVLIPFSAEEHMDFYNC"
     gene            8090..8413
                     /gene="yabA"
                     /locus_tag="VHI09_000637"
     CDS             8090..8413
                     /gene="yabA"
                     /locus_tag="VHI09_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEQ6120533.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            8418..9281
                     /gene="rsmI"
                     /locus_tag="VHI09_000638"
     CDS             8418..9281
                     /gene="rsmI"
                     /locus_tag="VHI09_000638"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEQ6120534.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSILEILSYL
                     EETPLKGECLLIVAGAQVDSEVELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            9308..9700
                     /locus_tag="VHI09_000639"
     CDS             9308..9700
                     /locus_tag="VHI09_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120535.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(9747..10376)
                     /locus_tag="VHI09_000640"
     CDS             complement(9747..10376)
                     /locus_tag="VHI09_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEQ6120536.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTTENQ
                     LDTDAIEQLLPATQGLPLVFHMAFDRIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQSLSQLTGTAIVHGTKII"
     gene            10676..11032
                     /locus_tag="VHI09_000641"
     CDS             10676..11032
                     /locus_tag="VHI09_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEQ6120537.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGK
                     VLQVGYRKPYQELDLG"
     gene            complement(11106..11933)
                     /gene="xth"
                     /locus_tag="VHI09_000642"
     CDS             complement(11106..11933)
                     /gene="xth"
                     /locus_tag="VHI09_000642"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEQ6120538.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDTQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHLHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYWLASNRIVDKIKRSEMISSGERQDHTPILLDIDL"
     gene            12167..13348
                     /gene="lctO"
                     /locus_tag="VHI09_000643"
     CDS             12167..13348
                     /gene="lctO"
                     /locus_tag="VHI09_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEQ6120539.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDHLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGETPHWFQFYYSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDQKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            13614..18557
                     /gene="scpC"
                     /locus_tag="VHI09_000644"
     CDS             13614..18557
                     /gene="scpC"
                     /locus_tag="VHI09_000644"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEQ6120540.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLMMMTTTTVAADELTTTSE
                     PTITNHAQQQAQHLTNTELSSAESQSPDTSQITPKTNREKEQPQGLVSEPTTTELADT
                     DAASMANTGPDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAH
                     QSMRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFGD
                     FDEDWENFEFDAEPKAIKKNKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKY
                     ESHGMHVTGIVAGNSKEAAATGERFLGIAPEAQVMFMRVFANDVMGSAESLFIKAIED
                     AVALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDP
                     LATNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTAKELENRADLNHGKAIYSESVD
                     FKNIKDSLGYDKSHQFAYVKESTDAGYKAQDVKDKIALIERDPNKTYDEMIALAKKHG
                     ALGVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVS
                     KAPSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIA
                     GASLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNI
                     DGAVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKDKTLRYDTELLTDHVDPQ
                     KGRFTLTSRSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRF
                     RDSQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGK
                     HFTGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNN
                     QDFAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTL
                     LGTAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDP
                     ETNRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQA
                     DGSFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKE
                     TLKDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKG
                     LKNNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQ
                     YVVTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVRE
                     EVFYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVED
                     RAGNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYY
                     NNSGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQ
                     ITAHFDHLLPEGSRVSLKTAQGQLIPLEQSLYVPKAYGKTVQEDTYEVVVSLPKGYRI
                     EGNTKVNALPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTVSATPTKTTTSAT
                     AKALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            19329..20036
                     /locus_tag="VHI09_000645"
     CDS             19329..20036
                     /locus_tag="VHI09_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEQ6120541.1"
                     /translation="MSKKKRKHYQVYEGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKRLHWYLLSSVML
                     TGLAFGTALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
BASE COUNT         6468 a         3969 c         4170 g         5679 t
ORIGIN      
        1 gccttttttg atgtgatttt taggatgtat taaaaaagaa atattagttt agaatgatta
       61 aaaacaatag cgagaatcat tctaaagtaa cgtcaacata ctctagaggt agtgtgtggc
      121 gattgctaaa tcaccagcat aagctacttg ttgtccttta acaatttgat aagcatctgc
      181 gcctttccca gcgataatca cagcatcttt tgcttcttgg caaagagaca tggctttttg
      241 aatagcttgc tcacggtcac taataatctc taccgggcgt gcgatatggc tggcaatttc
      301 ctgcgaaata tcttctggat cctcgaaatt agggtcatca gcagtgagaa tgacggtgag
      361 gtttggatgc tgatgaatca ccctaccaaa atcagctcgt ctgctctcgc ctttatttcc
      421 tggtgcacct aaaatcaaca ttaatttgcc tgtctgatgt tcttccacaa cgctgagtaa
      481 tttttctaag ctgtcaccat tatgggcata atcaacaaaa actttagcat gatttgtcat
      541 agtgaggact tccatacggc ctgggacgcg agtcttagcg ataccttttt gaatatcagc
      601 taggctagca cccaaacgaa ggcaggcaag tccagctgcc atagcatttt cttggttaaa
      661 atggccaatt aattgaatgt cataatggcc agctaattgt cctttagctt caaaggagaa
      721 ggcttggcta gtggtgatct ggttgtcaga caagggacca taaaacacat gctcttgatc
      781 cgctacctga tcagctagaa atgaaaaatg atccatcccg ctattgatca caacagctct
      841 gctattctcc attaaaagac gcttatgata gaaataatct tcaaacgttg gatgctcgat
      901 tgggccaata tgatcaggac taatatttag gaaaacccca acatcgaacg taagcccata
      961 gacacggtca acgaggtaag cctgactaga aacttccata atcaagtggg tcatgccatt
     1021 agtcacacac tcagccatca tagcaaaaag atccaagctt tcaggagttg taagttgtga
     1081 tttgaaaaag gtcttgccat ctaaggtggt attcatggta gaaaacatgg caggtttata
     1141 ggattctttc aacatgtggt aggcaaaata agcagccgtc gtctttccct tagtacctgt
     1201 aaaagctagt agtttgagtt tttcttgagg gttgccatag aaagccatgg caattagact
     1261 catagctttt ttgatatcag tgaccaaaac aacgggaata ccaagctcgt aatctacctc
     1321 tgaaatatag agttgtaatc cgttagtgat tgcttcttta aggtagtcag ctttgaaagt
     1381 tgccccttta gcaaaaaaaa gggtcttgcc atctacttga cggctatcgt aactcaagcg
     1441 ctcaaaactt aagccttgat aatggtagtg gtatccgtcg gcatcaagaa cctcacgaaa
     1501 attatggtct ttttttaaaa tgtctaataa ttgttcaatg gttatcatac ccttatttta
     1561 tccttaaagt tcttgtttta caagtcacaa gcaaaagttt ataataaaac taatagaaac
     1621 taggaaagaa tagatatgtc gacagaaaaa aaacaactga cacaagaaga attgatggtt
     1681 cagggagcag cctggtccac agctggtaat ttcattagcc gacttttggg agtgttatac
     1741 attattccct ggtatatttg gatggggcaa tacgcgattc aagccaacgc tcttttcaat
     1801 atggggtata atgtttatgc ttatttttta ttgatttcaa ccacaggact caatgttgcg
     1861 attgctaagc aggttgccaa gtacaattcc atgggacaaa ctgagcatag ctatcaatta
     1921 atccgaagta ccctaaaact catgcttggt ctgggcctta ttttttcagc cattatgtat
     1981 ctgggttctc ctttatttgc gagcttatct ggtggtgatg acacacttgt tcccattatg
     2041 cacagccttt ctttagcagt ttttattttt cctgtaatga gtgtgattcg aggtatcttt
     2101 caaggccaca ataatatcaa gccttatgcg atgagtcaga ttgctgagca actcattcgg
     2161 gttatctgga tgcttttgac cacctttttt atcatgaagt taggttcaaa agattatcta
     2221 tcggcggtaa cgcagtcaac cttggcagcc tttgtgggaa tgattgctag tatggctgtc
     2281 ttaggttact acttgtggaa gttaggcttg ctaaaagcaa tatgtcgtaa gcccaatcat
     2341 catattgata gtgacattaa aggactgttg cttgaaacat taaaagagtc tattccgttt
     2401 atcgtaacag gaagtgccat tcaagccttt caattgattg atcaatggac ttttgtcaat
     2461 actatgactc tttttacaga ttatagccgt tctcaattgc ttgttttatt tggttacttt
     2521 aacgcaaacc cagccaaaat tacaatggtt ttaattgcag tagcagcatc tattggtggt
     2581 gtaggcattg ccttattaac tgaaaattat gtcaaaaaag acatgaaagc agctgctcgc
     2641 ttaatcatta acaacattga aatgttagtg atgtttttgt tacccgctct tactggggca
     2701 attattttag caagacctct atattccgtt ttttacggag ctagcgagga tcgtgccatt
     2761 cacctctttg tggcggtcct cttccaaacc ttgttgatgg cgctttatac ccttttttca
     2821 ccgatgcttc aagctctttt tgaaaatcga aaagcgattt actactttgt ttatggtatc
     2881 ttgattaagt tagttttaca gataccgctt atttatttgc tacatgctta tggtccttta
     2941 ctagcgacga cgattgcttt agtggtgccg atttatttga tgtatcgacg cctatatcag
     3001 gttactcatt ttaaccgcaa actgttgcaa aaacgtttat tattaacctt gattgaaacc
     3061 ttattaatgg gactggtcgt gtttgtggcc aactggctat tgggctatgc ctttaaaccg
     3121 acaggccgct tgaccagcct tctttatctc ctcattattg gtggcgtggg aatgacggct
     3181 tacacggcac tcaccttgct gacgcatcaa ctagataaat taattggtag caaagctagt
     3241 cgccttcgtc agaaattagg ctggcattaa tataatgaca ccaagaacga tttcgtgagt
     3301 gcttatcgaa atcgtttttt tcttaatgat ctcgcttagt aaaggtagct gataaaaaat
     3361 caggatttcc caagcaaact actgatattt ttggtataat gataaaagtg taaaaaaata
     3421 gaaggaagta tatcaacatg ggaaaatgtc aagttatttc gcatccgctt atccaacaca
     3481 aactttcaat cttacgtcgt caaaccacat caactaaaga ttttcgtgag ttggttaatg
     3541 aaattgcgat gctgatggga tatgaagtgt cacgcgacct tcccttagaa gatgtcgata
     3601 tccaaacgcc agtctcaaaa acggtccaaa aacagttagc tggtaaaaaa ttagctattg
     3661 ttccgattct tcgtgctggt attgggatgg ttgatggctt gttaagtttg gtaccagctg
     3721 caaaagttgg ccatatcggc atgtaccgta atgaagaaac ccttgaacct gttgaatacc
     3781 tcgttaaact accagaagat attaaccaac gacaaatttt ccttgttgat ccaatgttgg
     3841 caacaggtgg ttcggctatc ttagcagtgg attcgttgaa aaaacgtggc gcagcaaata
     3901 ttaagtttgt ctgtttagtt gcagcaccag aaggggtgaa aaaacttcaa gaagctcatc
     3961 cagatattga tatcttcaca gcagcacttg atgatcattt aaatgaacat ggctatatcg
     4021 tgccaggctt gggcgatgct ggtgatcgtt tatttgggac aaaataaaag agacaaccat
     4081 caaatagtag tctttttagg ccaatcgttc ttgattttgt taaaaaaatc agagagattt
     4141 ctcttcttta aaggaaaagc aagtttatga tttgaccttt attgaccaaa atattataat
     4201 aaagacaatc agtaaaaaag agatgaccta gtgggttgcc gtctctcaaa atgacgctac
     4261 taaaaaccct tgtagattgg agaatgtatt atgattcctg ttgttattga acaaactagc
     4321 cgtggagaac gttcttatga catttattca cgtttattaa aagaccgtat tatcatgtta
     4381 acagggcctg tcgaagacaa catggccaac tctgtcatag cgcagctctt attccttgac
     4441 gcgcaagaca acactaaaga tatctaccta tatgtcaaca caccaggtgg ctctgtctca
     4501 gcaggcttgg ctattgtcga taccatgaac tttatcaagg ccgatgtgca aactattgtc
     4561 atgggtatgg cagcttctat ggggacagtc attgcttcat caggaacaaa aggcaaacgc
     4621 tttatgttac caaatgcaga gtatatgatc caccagccaa tgggtggcac aggcggtggc
     4681 acgcaacaaa cggatatggc tatcgcagca gaacatctct taaaaacacg tcaccgttta
     4741 gaaaaaatct tagcgcaaaa tgctggaaaa acgattaaac aaatccataa agatgctgag
     4801 cgtgattatt ggatgagtgc tgaagaaacc ttggcttacg gtttcattga tgaaatcatg
     4861 gaaaacaacg aactaaaata aagaggatct ctgcaaaagt attctagaaa cttctaggat
     4921 gctttttctc ttagaaagga aaataatgat acagcctact gcattaaaac agcttcaaga
     4981 gttaaaacgt cagttcagat ccctttgcaa ccaattatga gatagtttga ggatgatcct
     5041 tagcagctgt gtgcaagaag ttatgacgat ttcataaagg atgacctagc cagtctttct
     5101 tcgtctcttt tacttaaagt gtggtataat cagattatgc tagccacaac taaaaaatgc
     5161 attgccatta aaggtgttgt agatcggctt atcctagatc tcataacaat gctgtagtac
     5221 cgcccttacc ttgctactaa agacgctgtt gaagcacaaa ttgacttgca aaggatcttg
     5281 actgataggt acatgtccgg tggaatcgcc aaagcagcaa aaagaccaag acccttattt
     5341 ggtctctacc aaaatataat agaaaaggat ttagaatgtt tcaaaaacaa gaacgtattg
     5401 gactcgtcgt ttacctgtat tataaccgag atgccagaaa gttatcaaaa tttggtgatt
     5461 tatattacca ttccaagcgc tctcgttacc tcattattta tattaataaa aatgatttag
     5521 acacaaaatt agaagaaatg agacgtttga aatgtgttaa agacattaga ccatcagctt
     5581 ttgatgatat tgaccgccaa tttgtaggta accttcatcg cgacgaaaca aacaaccatc
     5641 aaaagggtta ccaaccccct agttactgat tagcgtttca gatttagtta gcttattaaa
     5701 gtaagaaggt tttgacagat gctcacacta cttcaagaca atgagacaac tagagcatta
     5761 gtgaataact gagttgactg tcacttcttt aataagaacg gataggattg gtcctaaaat
     5821 agagcactgc aaagatatgc cagtgctttt tagctcattt tttgataaaa tagaactact
     5881 aaaaagtgaa ggaaataaga tgatcacagg aaaactcata accgttgaag gtccagatgg
     5941 cgcagggaaa acgactgttt tagagcaact cattccactg ttaaaacaaa aagtggctca
     6001 ggagatcttg acaacaagag agcctggagg tgttgccatt tcagaatata ttagagaact
     6061 tattttagac atcaaccata ctactatgga ccctaaaaca gaactcttac tttatattgc
     6121 ggctcgaagg cagcatttgg tagaaaaggt actaccagct cttgaagcag gccggttggt
     6181 ttttattgat cgtttcatcg atagttcagt agcttatcaa ggtgcgggac gcggtttgat
     6241 caaagcagac attcagtggc tcaacgagtt cgcaacagat ggtttagagc ctgacttgac
     6301 cctttatttt gatgttcctt ctgaaattgg tttagcacgg attaacgcga accaacaaag
     6361 agaagtcaat cgtttagatt tggaaacaat agagatacat caacgagtac gaaaaggata
     6421 cctagcctta gctaaggagc atccaaaacg tattgtcaca attgatgcga ctaaaccatt
     6481 gaaagaagtt gtctctgtcg ctttggaaca tgtcttagcc ttgcttttag cctaggataa
     6541 gggagggtag gaatggattt agcgcaaaaa gctcctaacg tttatcaagc ttttcagacg
     6601 attttaaaga aagaccgtct gaatcatgct tatctttttt cgggtgattt tgctaatgaa
     6661 gaaatggctc tttttttagc taaggtcatc ttttgtgaac agaaaaagga tcagacgccc
     6721 tgcgggcatt gtcgctcttg tcaattgatt gaacaaggag attttgccga tgtgacggta
     6781 ttggaaccaa cagggcaagt gattaaaacg gatgtggtca aagaaatgat ggctaacttt
     6841 tctcagacag gatatgaaaa caaacgacaa gtttttatta tcaaagattg tgacaaaatg
     6901 cataccaatg ccgctaatag cttgctaaag tatattgagg agcctcaggg agaagcttac
     6961 atgtttttgc tgaccaatga tgataacaaa gtgcttccga ccattaaaag tcggacacag
     7021 gtttttcagt ttcctaaaaa cgaagcctat ctttaccaat tggcacaaga aaagggatta
     7081 ttaaaccatc aggctaagct agtagccaaa cttgccacaa acaccagtca tctagaacgt
     7141 ctgttgcaaa cgagcaagct tttagaactg ataactcaag cagagcgttt tgtatctatt
     7201 tggctgaaag atcagttgca ggcatattta gcgttgaacc gtctggtaca gttagcaact
     7261 gaaaaagaag aacaagattt agttttgacc cttttgacct tgctcttggc aagagagcgt
     7321 gcgcaaacgc ctttgacaca attggaggct gtttatcagg ctaggctcat gtggcagagc
     7381 aatgttaatt ttcaaaacac attagaatat atggtgatgt cagaataagt gcaattggaa
     7441 ttcgttatgg ggaaacgaag cttgtcaaaa gacttgcccg tagcgaccaa agtagcggcg
     7501 cataggggca aaaaagcttt tgaagaaaat actgcctttg caacagattt gatgaaagaa
     7561 gtatcacgat taataacaca aaataaactt gaaatgacgt ttattaatag tgtctttctc
     7621 ttagatcagc atgttgtcct aattcccttt tcagcagaag agcacatgga tttttacaac
     7681 tgttaaaaga tttagcggcc gattttaggt caagaagtga gctgccccag attaacagcc
     7741 aagaagaaac caagatttat ggtcgcttag gaccgtgtgg cagaccactg tgctgctttc
     7801 gtttttggga aaatttctga caatatctat taaaatacta aaaaatcaaa ggttatcatt
     7861 gactttgggt aaagcaacaa gcccttatgg ccgtttgctt tgctgtctct atgatgaagg
     7921 tggcttttac caataaagca agaaaaaatt ccctgaatat ggtaccatag tagagacaag
     7981 tgatggccct gcaacagtag cagccttgat atatttacag atcccattaa ggtgcggtta
     8041 aaagaccagt tgaccttagt cacttatgct ttagaggagg ttaaagtagg tgaataaaaa
     8101 agaattattc gatgcttttg atggattttc ccaaaattta atggttactt tagctgaaat
     8161 tgaagccatg aaaaaacaag tccaatcact agtagaagaa aacacgattc ttcgcctaga
     8221 aaatacaaaa ctacgggaac gtttgagtca cctagaacat gaaacggtag ctaaaaatcc
     8281 ttccaaacaa aggaaggatc acttagaggg aatttatgat gaagggtttc atatctgcaa
     8341 ttttttctat ggacaacgtc gtgaaaatga tgaagaatgt atgttttgca gagaattatt
     8401 agatagaaag taagggcatg caggttcaaa aaagttttaa agacaaaaaa acaagcggaa
     8461 ccttgtattt ggttccaacc ccaattggca atctgcagga catgaccttt agagcagtgg
     8521 caacgttaaa agaggttgat tttatttgtg cagaagacac aagaaacaca ggtcttttgc
     8581 taaaacactt tgacattgct accaagcaaa tcagctttca tgagcacaac gcctatgaga
     8641 agattcctga tttaattgat ttattaatat caggacggtc actagctcag gtatctgatg
     8701 caggaatgcc atcgatttct gatcctgggc atgatttggt caaggctgct attgatagtg
     8761 atatcgcagt cgttgcttta ccaggtgcct cagcagggat tacagctttg attgctagtg
     8821 gactggctcc tcaaccccat gttttttatg gatttttacc cagaaaagca gggcaacaaa
     8881 aggctttctt tgaggacaaa caccattacc cagagacaca aatgttttat gaatcgccct
     8941 atcgcatcaa agataccctg accaatatgt tggcttgtta tggtgatcgt caagtggtac
     9001 ttgttagaga attaaccaag ctttttgagg agtaccaacg ggggtcgatt ttagaaatct
     9061 tgtcctacct tgaggaaaca cccctaaaag gggagtgtct gctgattgtg gctggagccc
     9121 aagtagacag tgaggttgag ctgacagctg atgttgactt agtgtctcta gtgcaaaaag
     9181 aaatccaagc aggagccaag ccaaatcagg ctattaaaac aattgctaaa gcttaccagg
     9241 taaatcgcca agagctttac caacaatttc atgacttata agtcatcgta gattaggaga
     9301 aaaacaaatg acagaagatc aagcaactat ccttatttta ggagtatacg gaatcatatt
     9361 ggctgtattc ttattatcag ccttattatg ccttatcgct aactggcgac tctttaaaag
     9421 agcaggttac aaaggatggt atagtctcat tcctttctat gcaggttttg tcaaacacaa
     9481 aatcactttt ggagaagata ataaatggtt ttactttatt ggttgggtca ttgctattta
     9541 ttattactat gtgcactttt gctatgtccg tgcttttggt ggatcaaagg gtctagcagt
     9601 atttggcttg tttttcccag gcattgcttc tttgatagtg gcttttggaa aaacttatac
     9661 cacacaagaa tgccaccctg tgccacatct tttaaactaa gagaaaaaag accatcaatt
     9721 ggatttattg atggtctttt aggtgtttaa atgattttag tcccgtgaac aatggccgtt
     9781 ccagtgagtt gactcaagct ttggcaattc tcagcagtga cgcctcctcc aatcatgatt
     9841 tcaatgcgtt tattggcata ggtcacaaga cttttaagct gttccacatt atctgtaata
     9901 ggagtagctt caggggagcc atgagttagc acacgcacaa agccataatc aatcagttgg
     9961 tcaagcgctt ggtgctggtg atcagtcgga atccggtcaa aagccatgtg gaaaaccagt
    10021 ggcagtcctt gtgttgcagg cagtaattgc tcgatagcgt cggtatccag ttgattttct
    10081 gtggtcagca agcctaagac tagcgcatct gagccagctt caacagcttt taagatatct
    10141 tcttccatag cttttagttc gaggtcgtta tagacaaaat caccaccgcg aggtcgaatc
    10201 atggtcgcta cgctaatctt tttatcgtgt aagagctgac aggcttcttt gatgacgcca
    10261 taagatggtg ttgttccccc gacagctagg ttatcacaga gttccacacg actgacttga
    10321 ccagcatcaa gggttggcag tagtgtgagg ttctcagcac aaaattcttt aatcattttt
    10381 agatctcctt tatcactaat attatagcac gaatgaagct agtcatcagg aaacattttt
    10441 gtcaaatcat ggacattatg ctatactcac tagtattaaa agtaggccaa aacagcagca
    10501 aatactttga aaaaagattg ctatgttcta cggcaatatc agtctttacc aacgaactct
    10561 caagaagaaa agaaagacaa agcttagcat tttaaaaaag cccccccttt ttttgtgaaa
    10621 tgatgaccaa aaacaggcgc ctcacgccta aaacagttac cctaaaggag aacaaatgat
    10681 tacgatttac caatacccaa aatgtagcac ctgccaaaga gccatggctg aattaaagca
    10741 actggttagt gactttgaag ccattgacat caaagctaat ccacccaaag ctcaggacct
    10801 caaacattgg atggaaacat ctggttatac catcaaaaat ttctttaata ccagtggtaa
    10861 cagctaccgc gaattgggac ttaaagataa aatagaccag cttagccttg ataaagctgc
    10921 tgaactctta gccacagatg gtatgctgat taaacggcct attctgatta aggacggtaa
    10981 agtcctacaa gttggctatc gcaagccata tcaagagttg gatttaggat aatgcgacaa
    11041 agtctcaaaa aaggacaagg caacattata acgccttgtc cttttttggg atctgttttt
    11101 agacattaga gatcaatatc tagtaaaatt ggcgtatggt cttggcgttc cccagaagaa
    11161 atcatttcag aacgcttaat cttatccacg attcggtttg aagctagcca atagtcaatt
    11221 ctccaaccgg tattattgat tttgctggtt ttactacgtt gtgcccacca ggtataaaca
    11281 tgaggaatat cgccgtgcaa gtgacgaaaa gtatctgtaa agcctcttgc taataggtta
    11341 gtaaagcctt gacgttcttc gtcggtaaaa ccaggggaac gtcgattgct gcttggattt
    11401 gccaaatcaa tctctttgtg ggcaacgttg taatctcccg tagccaaaac aggcttttga
    11461 gtatccagtt cagtcaagta atcagcgtat ttgtggtccc agatttgacg gtcatctaac
    11521 cgtctaaggc catcaccagc attaggcgtg taaacctgtg tgacgaaaaa gtcttcaaat
    11581 tctagggtaa tgatacgacc ctcagcatcc attgttgttg gggcaccaat ttctggaaaa
    11641 gtaatgacag ggtttagcgt gtttttgtac aggaacatgg tgccagcata gcctttccta
    11701 gcaggctcaa ctgaagaacg ccacacatgc aaatagtttg gaaagtaact aagtagggtc
    11761 tcgatatgtt ttttggtagg ccccttagca gataatttag tttcttgaat ggcaataata
    11821 tccgcatctt gagcgactaa agtgtctaaa acagcgcgtg ataaaagcgc acgaggggac
    11881 tctcctgtga gagcagcatt taaggaatca atgttccatg aaatcagttt catttggtat
    11941 ctctttctaa cagatagtct tgttgttatt gtaacaaaaa acgaaacgat aagcgacttt
    12001 gggcacctag cccccataaa acagtctagc caaatgaagc atagcagcgt aagaacaaga
    12061 caaggaggtg atcagtgagc atatcgtttg acagttaaga gtaggattac tataatagaa
    12121 gcataaatgt aagcgctaac aaaataaaaa atataggaga atgtcaatgg cacaaaaaac
    12181 agtcatcacc gaagaaacaa ccgattttgt tatggatttc aaaacaagta gcgctgaggg
    12241 aaatgttgat tttattaacg tttttgacct tgagaaaatg gcacaacagg tcatccctaa
    12301 aggagccttt ggttatattg ctagtggtgc tggtgatacc tttaccctcc atgaaaatat
    12361 tcgctcattt aatcataaat taattgtccc tcactccctc aaaggggttg aaaatccgtc
    12421 aacagagatt acgtttgatg gcgatcactt aacctctcct cttattttag cacctgtagc
    12481 ggctcataaa ctggccaatg aacaaggaga agtagcgtct gccaaaggat tgaaggaatt
    12541 tggaagtatc tacaccacta gttcctactc aacaactgac ctgccagaaa tcagtgctgc
    12601 cttaggtgag acaccccatt ggtttcaatt ttattacagt aaagacgatg gcattaatcg
    12661 taatattatg gatcgtgtca aagcacaagg ttgcaaagct atcgttttga cagcagatgc
    12721 aacagttgga ggaaaccgcg aagttgatag gcgaaacggt tttgtttttc cggtcggtat
    12781 gccaattgtt caagaatatt tacctgatgg tgcaggtaaa acaatggatt atgtctataa
    12841 atcagctaaa caagcgctta cctcaaaaga tattgaatat atcgcaacct actcaggcct
    12901 gcctgtttat gtcaaaggac cacagtgtgc tgaggatacc ttgcgggcat tggacgcagg
    12961 agcatcaggc atctgggtaa ccaatcatgg tggccgtcaa ttagatggtg gtccagctgc
    13021 ttttgattcc ttacaagaag tcgctgaagc agttgatcaa aaagttccta ttgtctttga
    13081 ttcaggtata agacggggtc aacatatttt caaagctctt gcttcaggtg ctgaccttgt
    13141 tgccttgggg cgtccagcga tttatggctt ggctatggga ggaagcattg gtactcgtca
    13201 ggtctttgaa aagttaaatg acgagttaaa aatggtcatg caactggctg ggacacaaac
    13261 tatccaagat gttaaagcct ttaaccttcg ccacaatccg tatgattcat cgattccatt
    13321 tgatcaaaat gcgctacgct tagattagaa cagacaaaaa cctctgctat catgtcacaa
    13381 atagtagagg ttttacactg caaaaagagg tgtgaaaatt caataacata gttacaaggc
    13441 tttcgtttaa caccccccga taatagatta aaatgaaaga cttcttacat taatagaagt
    13501 ttctgttgat tttctcagaa aaatgtggtt aaataatcaa atattattaa cgaaaaatga
    13561 gttaacgatt aacattaaac tggttttagt taaaacattt aggagggtat caggtggaga
    13621 aaaagcaacg tttttccctt agaaaataca aatcaggaac gttttcggtc ttaataggaa
    13681 gcgttttctt gatgatgatg acgacaacaa cagtagcagc agatgagcta accacaacga
    13741 gtgaaccaac aatcacgaat cacgctcaac aacaagcgca acatctcacc aatacagagt
    13801 tgagctcagc tgaatcacaa tccccagaca catcacaaat cactcccaag acaaatcgtg
    13861 aaaaagagca accacaaggt ctagtctctg agccaaccac aactgagcta gctgacacag
    13921 atgcagcatc aatggctaat acaggtcctg atgcgactca aaaaagcgct tctttaccgc
    13981 cagtcaatac agatgttcac gattgggtaa aaaccaaagg agcttgggac aagggataca
    14041 aaggacaagg caaggttgtc gcagttattg acacagggat cgatccggcc catcaaagca
    14101 tgcgcatcag tgatgtatca actgctaaag taaaatcaaa agaagacatg ctagcacgcc
    14161 aaaaagccgc cggtattaat tatgggagtt ggataaatga taaagttgtt tttgcacata
    14221 attatgtgga aaatagcgat aatatcaaag aaaatcaatt cggggatttt gatgaggact
    14281 gggaaaactt tgagtttgat gcagagccaa aagccatcaa aaaaaacaag atctatcgtc
    14341 cccaatcaac ccaggcaccg aaagaaactg ttatcaaaac agaagaaaca gatggttcac
    14401 atgatattga ctggacacaa acagacgatg acaccaaata cgagtcacac ggtatgcatg
    14461 tgacaggtat tgtagccggt aatagcaaag aagccgctgc tactggagaa cgctttttag
    14521 gaattgcacc agaggcccaa gtcatgttca tgcgtgtttt tgccaacgac gtcatgggat
    14581 cagctgaatc actctttatc aaagctatcg aagatgccgt ggctttagga gcagatgtga
    14641 tcaacctgag tcttggaacc gctaatgggg cacagcttag tggcagcaag cctctaatgg
    14701 aagcaattga aaaagctaaa aaagccggtg tatcagttgt tgtagcagca ggaaatgagc
    14761 gcgtctatgg atctgaccat gatgatccat tggcaacaaa tccagactat ggtttggtcg
    14821 gttctccctc aacaggtcga acaccaacat cagtggcagc tataaacagt aagtgggtga
    14881 ttcaacgtct aatgacggcc aaagaattag aaaaccgtgc cgatttaaac catggtaaag
    14941 ccatctattc agagtctgtc gactttaaaa acataaaaga tagcctaggt tatgataaat
    15001 cgcatcaatt tgcttatgtc aaagagtcaa ctgatgcggg ttataaagca caagacgtta
    15061 aagataaaat tgctttaatt gaacgtgatc ccaataaaac ctatgacgaa atgattgctt
    15121 tggctaagaa acatggagcc ctgggagtac ttatttttaa taacaagcct ggtcaatcaa
    15181 accgctcaat gcgtctaaca gctaatggga tggggatacc atctgctttc atatcgcacg
    15241 aatttggtaa ggccatgtcc caattaaatg gcaatggtac aggaagttta gagtttgaca
    15301 gtgtggtctc aaaagcaccg agtcaaaaag gcaatgaaat gaatcatttt tcaaattggg
    15361 gcctaacttc tgatggctat ttaaaacctg acattactgc accaggtggc gatatctact
    15421 ctacctataa cgataaccac tatggtagcc aaacaggaac aagtatggcc tctcctcaga
    15481 ttgctggcgc cagccttttg gtcaaacaat acctagaaaa gactcagcca aacttgccaa
    15541 aagaaaaaat tgctgatatc gttaagaacc tattgatgag caatgctcaa attcatgtta
    15601 atccagagac aaaaacgacc acctcaccgc gtcagcaagg ggcaggatta cttaatattg
    15661 acggagctgt cactagcgga ctttatgtga caggaaaaga caactatggc agtatatcat
    15721 taggcaacat cacagatacg atgacgtttg atgtgactgt tcacaaccta agcaataaag
    15781 acaaaacatt acgttatgac acagaattgc taacagatca tgtagaccca caaaagggcc
    15841 gcttcacttt gacttctcgc tccttaaaaa cgtaccaagg aggagaagtt acagtcccag
    15901 ctaatggaaa agtgactgta agggttacca tggatgtctc acagttcaca aaagagctaa
    15961 caaaacagat gccaaatggt tactatctag aaggttttgt ccgctttaga gatagtcaag
    16021 atgaccaact aaatagagta aacattcctt ttgttggttt taaaggacaa tttgaaaact
    16081 tagcagttgc agaagagtcc atttacaggt taaaatctca aggcaaaacc ggtttttact
    16141 ttgatgaatc aggtccaaaa gacgatatct atgtcggtaa acactttaca ggactcgtca
    16201 ctcttggttc agagaccaat gtgtcaacca aaacgatttc tgacaacggt ctacacacac
    16261 ttggtacctt taaaaatgca gatggcaaat ttatcttaga aaaaaatgcc caaggaaacc
    16321 ctgtcttagc catttctcca aatggtgata acaaccaaga ttttgcagcc ttcaaaggtg
    16381 ttttcttgag aaaatatcaa ggcttaaaag caagtgtcta ccatgctagt gacaaggaac
    16441 acaaaaatcc actatgggtc agcccagaaa gctttaaagg agataaaaac tttaatagtg
    16501 acattagatt tgcaaaatca acgaccctgt taggcacagc gttttctgga aaatcgttaa
    16561 caggagctga attaccagat ggatattatc attatgtggt gtcttattac ccagatgtgg
    16621 tcggtgccaa acgtcaagaa atgacatttg acatgatttt agaccgacaa aaaccggtac
    16681 tatcacaagc aacatttgat cctgaaacaa accgattcaa accagaaccc ctaaaagacc
    16741 gtggattagc tggtgttcgc aaagacagtg tcttttatct agaaagaaaa gacaacaagc
    16801 cttatacagt tacgataaac gatagctaca aatatgtctc agtagaagac aataaaacat
    16861 ttgtggagcg acaagctgat ggcagcttta tcttgccgct tgataaagca aaattagggg
    16921 atttctatta catggtcgag gattttgcag ggaacgtggc catcgctaag ttaggagatc
    16981 acttaccaca aacattaggt aaaacaccaa ttaaacttaa gcttacagac ggtaattatc
    17041 agaccaaaga aacgcttaaa gataatcttg aaatgacaca gtctgacaca ggtctagtca
    17101 caaatcaagc ccagctagca gtggtgcacc gcaatcagcc gcaaagccag ctaacaaaga
    17161 tgaatcagga tttctttatc tcaccaaacg aagatgggaa taaagacttc gtggccttta
    17221 aaggcttgaa aaataacgtg tataatgact taacggttaa cgtatacgct aaagatgacc
    17281 accaaaaaca aacccctatc tggtctagtc aagcaggcgc tagtgcatcc gctattgaaa
    17341 gtacagcctg gtatggcata acagcccgag gaagcaaggt gatgccaggt gattatcagt
    17401 atgttgtgac ctatcgtgac gaacatggta aagaacatca aaagcagtac accatatctg
    17461 tgaatgacaa aaaaccaatg atcactcagg gacgttttga taccattaat ggcgttgacc
    17521 actttactcc tgacaagaca aaagcccttg gctcatcagg cattgtccgc gaagaagtct
    17581 tttacttggc caagaaaaat ggccgtaaat ttgatgtgac agaaggtaaa gatggtatca
    17641 cagttagtga caataaggtg tatatcccta aaaatccaga tggttcttac accatttcaa
    17701 aaagagatgg tgtcacactg tcagattatt actaccttgt cgaagataga gctggtaatg
    17761 tgtcttttgc taccttgcgt gacctaaaag cggtcggaaa agacaaagca gtagtcaact
    17821 ttggattaga cttaccggtc cctgaagaca aacaaatagt gaactttact taccttgtgc
    17881 gggatgcaga tggtaaaccg attgaaaacc tagagtatta taataactca ggtaacagtc
    17941 ttatcttgcc atacggcaaa tacacggtcg aattgttgac ctatgacacc aatgcagcca
    18001 aactagagtc agataaaatc gtttccttta ccttatcagc tgataataac ttccaacaag
    18061 ttacctttaa gatgacgatg ttagcaacct ctcaaataac tgcccacttt gatcatcttt
    18121 tgccagaagg cagtcgcgtt agccttaaaa cagctcaagg tcagctgatc ccgcttgaac
    18181 agtccttgta tgtgcctaaa gcttatggca aaactgttca agaagacact tacgaagttg
    18241 ttgtcagcct gcctaaaggc taccgtatcg aaggcaacac aaaggtgaat gccctaccaa
    18301 atgaagtgca cgaactatca ttacgccttg tcaaagtagg agatgcctca gattcaactg
    18361 gcgatcataa ggttatgtca aaaaataatt cacaggcttt gacagtctct gccacaccaa
    18421 ccaagacaac gacctcagca acagcaaaag ccctaccatc agcgggtgaa aaaatgggtc
    18481 tcaagttgcg catagtaggt cttgtgttac tcggacttac ttgcgtcttt agccgaaaaa
    18541 aatcaaccaa agattgataa caaaacgcaa agagacaaca aatcatccct tttaacagtc
    18601 ggacctttct ttgaaattga gctggcagat gatcatttct taacaaaagt taaagagttg
    18661 agcaaagtaa ggaacagaca taaagatcat aaaagcaagc cagtttggct tgcttttatg
    18721 gtgttattgt cgtgtgctac gatgactaaa aaagaacaac gaacaatgac ctcgaatcac
    18781 caaaaggact tgatttgata gcttccctat gctatactaa gcctattctt tcaagaagga
    18841 ctccttttgt ggaaaagact gaaaaaaagg acactaatct atccaaaaac tccagatgag
    18901 tagctgcaac cggatgcctc tgctgtgatc aaggtgataa agcagactgg cttgtcacta
    18961 cctgagcagg tgtcaaaaaa agaagctttg gctttgtcat tcaacatact gagctttagt
    19021 ctttcagaac ggagacaccc ccagttttgt actcaaaaag cataaacagc tctgctgatg
    19081 cgacatccga tttctacgtc cgcagtgagc caaaccagct gctgtttgct atggtaaaag
    19141 tggcgccaat cgggattttt gagatcctag gctcaaaaat aaggcataga attccaaagg
    19201 aagtcgctgc cgtaagcacc acctgagaaa aagtatccca aaagactttt tctgcaattt
    19261 gaaatactga atttcagtat tttttctttt gtaactaaga gcaatgaatg aggtaaaaaa
    19321 aggcaacaat gtcaaaaaag aaacgaaaac actatcaggt ttatgaggga ttacgctgtg
    19381 ctatgacgct ttgttttatc agtggctatg tcaatgcctt tacctatatg acccaaggaa
    19441 aacgttttgc aggtgttcag acaggcaacc ttttgtcatt tgccattcgc ttgtcagaac
    19501 agcaactgaa ggaggcactg caatttttac ttcccatgat agtttttatg cttgggcagt
    19561 cctttactta tttcatgcac cgctgggcca ctaaaaaaag gctccactgg tacctcttat
    19621 caagcgttat gttaacagga cttgcttttg gaacagccct attcacgcct tttcttcctt
    19681 ccaatgttac ggtagcagct ttagcctttt ttgcctctat tcaggttgat acgtttaaaa
    19741 ccttacgagg agctagctat gctaatgtga tgatgacagg aaatatcaaa aatgccgcct
    19801 atttactaac caaaggcttg tacgaaaaaa atcacgaact aacacatatc ggacgcaaca
    19861 ccttaatagt catcttggct tttgctgttg gggtggtttg ttcgacgttg ctttgcatag
    19921 cttatggtga gtatgctttg atgcctattt taatgccttt actctatgtc aattacctgt
    19981 tggctcagga attttatcac attcaaacca aaatcaaacc gattaaaaga acttaacgga
    20041 cttgtggaga aagaaagttc atttagggta agtttttact caccctcctt tgcacgccaa
    20101 acgcatgaag aaacttttgt tttcagaaaa ctagattcat aatgacaaaa tcaatcaaag
    20161 tagtacacta tagatgaggt gactacgatg attgatttta ttatttctat tgatgattgc
    20221 gcagttgaat tggatagtcg tcaatcttgg aaaattcgct cccccttatc aaccattcta
    20281 tttctt
//
LOCUS       DAUELZ010000023        20223 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.023, whole genome shotgun sequence.
ACCESSION   DAUELZ010000023 DAUELZ010000000
VERSION     DAUELZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20223
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.023"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            324..758
                     /locus_tag="VHI09_000646"
     CDS             324..758
                     /locus_tag="VHI09_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120542.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKNSAKKLDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEEKWQDTKDAVEVYGGIATE
                     TVEESLGQVKEKVEGIGEATSQTIQSKMEKLTSDKTDADDEK"
     gene            complement(808..1719)
                     /locus_tag="VHI09_000647"
     CDS             complement(808..1719)
                     /locus_tag="VHI09_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ6120543.1"
                     /translation="MKTVRIFYNPNSGKKEDQLAGQVKDYFCQHGFSEDSVEIITPKD
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAQCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIKQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGDFSKAEEISHFQANTLSLLPQAKKQAILPRARI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(1836..2486)
                     /locus_tag="VHI09_000648"
     CDS             complement(1836..2486)
                     /locus_tag="VHI09_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEQ6120544.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(2483..2923)
                     /locus_tag="VHI09_000649"
     CDS             complement(2483..2923)
                     /locus_tag="VHI09_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEQ6120545.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLSQEVTQ"
     gene            3142..3990
                     /gene="ylqF"
                     /locus_tag="VHI09_000650"
     CDS             3142..3990
                     /gene="ylqF"
                     /locus_tag="VHI09_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEQ6120546.1"
                     /translation="MAMIQWFPGHMSKARRQVQENLKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIKRLRDRGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTHY
                     PSRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYSLFVKEVRDGKLGQYTLDQV
                     GDMDAD"
     gene            3980..4771
                     /locus_tag="VHI09_000651"
     CDS             3980..4771
                     /locus_tag="VHI09_000651"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEQ6120547.1"
                     /translation="MPTSIKAIKESLEAVTSLLDPLFQELATDARLGVQKALKSRQKA
                     IQTDLAEEERLEAMLSYEKALYKKGYQAIAGIDEVGRGPLAGPVVAACVILPKYCKIK
                     GLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSIIKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            4836..5672
                     /gene="dprA"
                     /locus_tag="VHI09_000652"
     CDS             4836..5672
                     /gene="dprA"
                     /locus_tag="VHI09_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEQ6120548.1"
                     /translation="MNHFELYKLKKAGLTNKNILNILDYQEHQEKSLSLRDMAVVSGC
                     KHPSHFIEAYKQLDIQKLKMEFKQFPSISILDKHYPMALKEIYNPPVLLFFQGNLDLL
                     EKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
                     AIIGTGLDRFYPKENRELQTFLGKNHLVLTEYGPREEALSYHFPERNRIIAGLSRGIL
                     VVEAKNRSGSLITCQIGIEEGRDIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILS
                     EY"
     gene            5779..7908
                     /gene="topA"
                     /locus_tag="VHI09_000653"
     CDS             5779..7908
                     /gene="topA"
                     /locus_tag="VHI09_000653"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEQ6120549.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKKLP
                     EMAEGETVKKISTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(7984..8868)
                     /locus_tag="VHI09_000654"
                     /pseudo
     CDS             complement(7984..8868)
                     /locus_tag="VHI09_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8881..9447
                     /locus_tag="VHI09_000655"
                     /pseudo
     CDS             8881..9447
                     /locus_tag="VHI09_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
     gene            9462..10454
                     /locus_tag="VHI09_000656"
     CDS             9462..10454
                     /locus_tag="VHI09_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEQ6120550.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESSLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWGLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGFKVVGYDIYQSDAAKAVLDYKESVEEAIKDADLVSLHMPPTAENTHLFNA
                     DLFKSFKKGAILMNMARGALIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            10559..11224
                     /locus_tag="VHI09_000657"
     CDS             10559..11224
                     /locus_tag="VHI09_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEQ6120551.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLALRTGHHNQDDVLNSLLAAGDAIKASWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     ADD"
     gene            11217..11933
                     /locus_tag="VHI09_000658"
     CDS             11217..11933
                     /locus_tag="VHI09_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEQ6120552.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHVTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHALIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILLITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAEATIGILERIVIGVCMIMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            12084..13430
                     /gene="trmFO"
                     /locus_tag="VHI09_000659"
     CDS             12084..13430
                     /gene="trmFO"
                     /locus_tag="VHI09_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEQ6120553.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAESVTAELENHPLIEVIRDEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHDALTTAEEAPL
                     NAFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRSNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYAAIASRALADLDTCLASL"
     gene            13583..14989
                     /locus_tag="VHI09_000660"
     CDS             13583..14989
                     /locus_tag="VHI09_000660"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEQ6120554.1"
                     /translation="MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTIL
                     DKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHY
                     ADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
                     TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSG
                     ISQMTYLAAVEAGADRIDTALSPFSEGTSQPATESMYLALKEAGYDITLDETLLEQAA
                     NHLRQARQKYLADGTLDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEDVLA
                     EVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGEYGKTPAPV
                     NEDLQKSQIGSTTLTTNRPADQLNPEFEVLKAEIADLAQTDEDVLTYALFPSVAKTFL
                     TTKYQTDDVIKVTAFIKA"
     gene            15032..15346
                     /locus_tag="VHI09_000661"
     CDS             15032..15346
                     /locus_tag="VHI09_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120555.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKPLL
                     PQASPKSLQDKEATQASGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVVAVER
                     KS"
     gene            15346..15696
                     /locus_tag="VHI09_000662"
     CDS             15346..15696
                     /locus_tag="VHI09_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEQ6120556.1"
                     /translation="MKRYEVTVNGQVYEVSLRELADGETVEVSQSAAPATEKEMNTNA
                     AGGGLQVKAPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            15706..16836
                     /locus_tag="VHI09_000663"
     CDS             15706..16836
                     /locus_tag="VHI09_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEQ6120557.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLACFFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAGLMDHPANGNPGGLLYYLYKGTSLGIYPPLIFLCLGASTDFG
                     PLIANPKTILLGGAAQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLA
                     PDLLSTIALAAYSYMALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVT
                     IFVSLLVPSATTLVGCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANG
                     DLFLSATTLKIIALGLIAFAAGTAGGVLMGKIMYYLSGNKVNPMIGAAGVSAVPMAAR
                     VVQKVGQEEDPSNFLLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(17037..17921)
                     /gene="citG"
                     /locus_tag="VHI09_000664"
     CDS             complement(17037..17921)
                     /gene="citG"
                     /locus_tag="VHI09_000664"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEQ6120558.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFM
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGRFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(17914..18609)
                     /locus_tag="VHI09_000665"
     CDS             complement(17914..18609)
                     /locus_tag="VHI09_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ6120559.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKKTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQTYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(18767..20170)
                     /locus_tag="VHI09_000666"
     CDS             complement(18767..20170)
                     /locus_tag="VHI09_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEQ6120560.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYIPQ"
BASE COUNT         6402 a         3748 c         4094 g         5979 t
ORIGIN      
        1 atagataatt ttacttcaat ttttgattga aaatactaaa ataagtgatg tggtcacttt
       61 gacaaccatt tattggtcct tttagataag acaattttgt ggctcttcag ttgaaaggat
      121 gtgttgttga tgttagagga aacaatcatg ttaaattaac tcaggcttaa agaccaaaaa
      181 aggagtgaag tctattatga aaaaaagaaa ttttaaactt acagaaatgt tacaattggt
      241 tttattagtg gttcctgctg taaaacttgc taaaaaaatg tttaacgata gtaaaacacc
      301 gtctaaaaga aagtaggtct acaatgacta aaaaaaatca tggcgttttt tgggggttgt
      361 tgcttggatc agcagcagct agcattgctt acttaagttt atcgtcttcc aaaaaagatc
      421 aactcttgaa aaatagtgct aaaaaattag atgatctcaa cgcttacttg caagataaaa
      481 gtaagcaagt tctggatgca gtatcggaaa aagtacaaga atccaaagat gctgttgaag
      541 tttatggtgg tattgccgca gaaaccgttg aagaaaagtg gcaagatacc aaagatgctg
      601 ttgaagttta cggtggtatt gccacagaaa cagttgaaga atctttggga caggttaaag
      661 aaaaggtaga aggaattggc gaagctacta gccaaactat ccaatctaaa atggaaaaac
      721 tcaccagcga taagacagat gctgatgatg aaaaataggt tttaaaatac ctttaagata
      781 agacaaaaag tgccattagg cactttttta atcttctgtt ggaacaataa tcgaaatggc
      841 ttttggaatg atatctaatt gaatgggcag atagtcactt ttatccccat caatcctggc
      901 acgtggtaat atcgcttgtt ttttagcttg tggtagtaag ctaagcgtat tggcttgaaa
      961 atgactgatt tcctctgctt tggaaaaatc accctgttta aaatcattaa tatgccataa
     1021 aaatttaaag aaagagactt ttttcatggt ataaacttgg aaatagccat cgtcagcttg
     1081 agcaccagga gaaaaagaag gaaaaccagc aatggtattt gtcatcgtga ttaagagaaa
     1141 cttcgttttc aggtgaatac gatggttgtg gctaattaaa gagatggcat agctgcgatt
     1201 tcgtttcagt attcgaatac tatcttttaa aaatgctaga ggaccaaagc gtcgtttttc
     1261 ctcagccgtt acgtcggcag caatatcagc cagtaatcct aaggttaaac tggaaatcat
     1321 gtattgctgg ttagctttgc aaatatcaac ttgcttgatt tgtcccgtca agatagtatc
     1381 caacgcctca gtaatttgta aagggatatg catagccttg gcaaaattat tgactgtccc
     1441 tgagggcact aagcctatta aacattgagc tcctccttca tagatgccac cgatgatttt
     1501 gtttagtgtc ccatcgccac caagagggat aaccaagtca atcttgtcct tagcagcttg
     1561 ttttgctaat tgaaaagcct ggtcagcatc tttaggggta ataatctcta cagaatcttc
     1621 ggaaaaacca tgttggcaaa aatagtcttt gacttgcccg gcaagttggt cttctttctt
     1681 tccagaatta ggattgtaaa aaatccgaac ggttttcatc aggtctctcc tttgacagct
     1741 tattacttgc tatccattat accacttcta agaattgatg tcttacacca actcatcaca
     1801 ttaagcctct caatcagtct gagcatctat ttgtcctaaa gcataaagaa agtgatagcg
     1861 ataaattgga gggcagaggc caataaaata aagagatgcc aaatcatgtg gaagtagggt
     1921 ctttttttgg cataaaatac cgctccaatg gtataagaca agccaccaaa taacatgaga
     1981 agaccaaaag caagactagt tttttgaatg atgactggca agataaacac aacgagccag
     2041 cccataacga tataaagcat caaactgaat ttttcattga tacgtttggc aaaaattttg
     2101 tataaaatcc caaaaagcgt aatgccccac tgcaaaacaa taatgatata gcctagccaa
     2161 ccagaaacaa gggataaagc aacaggtgta taacttcctg cgatagcaat gtaaatcata
     2221 ctgtgatcaa taatgcgtag aatgtatttg tgaaccgaac cataagccat ggagtggtaa
     2281 atcgtagagg acaaaaacat caaaaagaga ctgatgacaa agatggagat accaatagcg
     2341 gctttaaggt cataagtttg ataagcatag ctggctgaaa taggaagtag aatgagcata
     2401 gcaaatgctc cgatagcgtg tgtcacgctg tttgctactt cctcactaaa agataagggt
     2461 aaactttgtt taaaggtttg gttcattggg tcacctcctg tgataaataa atagttttgt
     2521 ggtaatcttt gaccgtttga caggcaacct gcacaatcgg tggaatgtcc tcctcaatgg
     2581 gagctttctg attccaacag gttacaaaag tattgtagag actttcagaa tcagcctgtg
     2641 cctgaagttc ctccaatacg cgactaatat cttgaatagg aaataccgtt ttaaacaaac
     2701 tgatggcaat taagcgcgct agttgttgct tttgatattt tttcttgata ggtttagtca
     2761 catacccatg cttgacgtaa ttattaatca tagatgccgt tagtgattta ttatctgaca
     2821 cttctgaaaa atcagtgcac tggttgacat aaagtaacac ttgatccaaa tataagtcta
     2881 aatcagggag gtccttccaa taaggtaatg atggaaaaat cataggttcg tctctctatc
     2941 tagttttgat aactagatta tatgattaat gaaaacttat tgcaagcaaa aactgtaaag
     3001 acttaaaaga ggaaagtaga aatggcgaag tgaagctaat catgctataa tgttaaagaa
     3061 ttgattggga tagatgcctt ctgtcgcaat cagactattt tatcacgaag aagatgatgc
     3121 tacgaaagga atgatacact catggcaatg attcaatggt ttcctgggca catgtccaaa
     3181 gccagaagac aggttcagga aaatttaaag catgttgact ttgtaactat tttagtggat
     3241 gcacgcttgc cactttctag tcaaaatccc atgctcacca aaattgttgg tgataaacct
     3301 aaattaatga ttttaaataa agctgacttg gcagatgcaa ctcgaactaa agagtggaaa
     3361 gcctactatg aaagtcaagg cattaaaaca ctagccatca attctaaaga acaatcaaca
     3421 gttaaaaaag taacagaagc agccaaggaa ctcatggctg ataaaattaa gcgcctgcgt
     3481 gatcgtggta ttcaaaaaga aaccttgcga accatgatta ttggcattcc aaatgctggt
     3541 aaatctacct tgatgaatcg cttggctggt aagaaaattg ctgtggttgg taataaacct
     3601 ggtgtgacca aagggcaaca atggttgaag tccaataagg aattggaaat tctagatacc
     3661 cctggtattt tatggccaaa atttgaggac gaattggttg gcttaaaatt agctttaaca
     3721 ggtgctatca aggaccaatt actaccaatg gatgaggtca ctatttttgg tttgaattac
     3781 tttaaaacac attatcctag tcgccttact aagcgtttta agaatattcc tcttgaggaa
     3841 gaagctcctg aaatcattat gaccttaaca cgtcagcttg gctttaaaga tgattacgac
     3901 cgtttctaca gtttattcgt taaggaagtt cgtgatggta aattaggtca atacacctta
     3961 gatcaggtgg gagatatgga tgccgactag tattaaagct attaaagaaa gcttagaggc
     4021 cgttactagc ctcttggacc ccctctttca agaattggca actgacgcta ggttaggcgt
     4081 ccaaaaagcg ctaaaaagtc gacaaaaggc tattcagacc gatttagcag aggaagaacg
     4141 attagaagcc atgctttctt atgaaaaagc gctttataaa aaaggttatc aagccattgc
     4201 aggtattgac gaggtgggac gtggtccctt agcaggtccc gttgtggcag cttgtgtgat
     4261 tttacctaag tattgtaaaa ttaaaggcct taatgattct aaaaaaatcc ctaaagctaa
     4321 gcatgagacc atttatcagg cagtgaaaga aaaagctttg gctatcggta tcggtattat
     4381 tgacaatcag ctgattgatg aggtcaatat ttatgaagca accaaactag ctatgctaga
     4441 agccattaaa cagttggagg gccaactcac acagccagat tatctcttga ttgatgccat
     4501 gacattggat attgctattt cgcagcagtc tattattaaa ggcgatgcca attccttgtc
     4561 tattgcagca gcatcaattg tagctaaggt caccagagat cagatgatgg ctaactatga
     4621 tcgcattttt cctggttatg gctttgctaa aaatgcaggc tatggcacca aagaacattt
     4681 acaaggatta aaagcttacg gcataacgcc tatccatcgt aaaagttttg aacctgttaa
     4741 atccatgtgc tgcgattcaa ctaatcctta aaaagttctg tttttagcag aatttttttc
     4801 ttttttacga ataaatgaag taaggaggta acttagtgaa tcattttgaa ctttataagc
     4861 taaagaaagc tggactgact aataaaaaca ttctcaatat tcttgactac caagaacacc
     4921 aggaaaaatc gttgtcactt cgagatatgg ccgttgtttc tggttgtaag catccgtctc
     4981 actttataga agcctataag cagttagata ttcaaaaatt aaaaatggaa tttaaacaat
     5041 ttcctagtat ttctatctta gataagcatt acccaatggc attgaaagaa atatacaatc
     5101 cacctgtcct cttgtttttt cagggaaatt tagaccttct tgagaaacct aaattagcca
     5161 ttgttggctc cagacgctca agcgataccg gagtaaagtc tgtccgtaaa attcttaaag
     5221 aactcgggaa ccgttttgtg attgttagcg gacttgctcg aggtatcgac actagtgctc
     5281 atttagcctg ccttaaaaat gggggacaaa ccatcgctat tattggaaca gggttggatc
     5341 gcttttaccc taaagaaaat agggagttgc aaactttctt agggaaaaat catcttgtgc
     5401 taacagaata cggtccaaga gaagaagctt tatcttatca ctttccagaa cggaatcgta
     5461 ttatcgcagg ccttagccga ggtattcttg tcgttgaagc aaaaaatcgt tcaggttcct
     5521 tgattacttg tcaaattggg atagaagaag gccgagacat ttttgctgtc ccaggaaaca
     5581 ttttggacgg gaaatccgaa ggttgccttc agttaattaa agagggagca acatgcgtca
     5641 catcgggcat ggatatcctt tcagagtact aaaaaagtta aagtttcttc ctatatagca
     5701 tttttttatt gacagtttct taaaaatggg atattatgct ataagcttta aacaaactgt
     5761 tataggaagt gtgtcgaatt gggaacaaaa actacaacta agccaaaaac gggtactaaa
     5821 aagtccacca ctaaaaagaa atcaacagcg aaaaaaaatc tcgttattgt tgaatcacct
     5881 gccaaagcta agactattga aaaatatctt ggacgcagtt ataaagttgt tgcttcggta
     5941 ggacatattc gtgacttaaa aaaatcgtct atgtctattg attttgataa taattatgag
     6001 ccacaataca ttaatatccg tggtaaggga ccccttatca attctttaaa aaaagaagcc
     6061 aaagctgcta aaaaagttta tttagctagt gacccggatc gtgagggaga ggctatttct
     6121 tggcatttgt cacatatctt aggacttgat cctcaagata ataaccgagt ggtttttaat
     6181 gaaattacga aagatgcagt taaacatgcc tttgtggagc ctcgtcagat tgatatggac
     6241 ttggtagatt ctcaacaagc acgtcgtgtt ttggaccgta ttgtgggata ttccatttca
     6301 ccaattcttt ggaaaaaggt caaaaaagga ctttcagcag gacgtgtcca gtcggttgct
     6361 ttaaaactca tcattgaccg tgaaaatgac atcaaagcat ttgttccaaa ggaatactgg
     6421 tctattgatg gtctttttaa aaaaggaact aaaaagtttc aagcgacttt ttacggtatt
     6481 aatggcaaaa aaacaaagct tgataataac aacgatgtca aagaggtatt agcaaagcta
     6541 acaaatgaag attttcttgt cagcaaggtt gacaagaaag agcgacgtcg taatgctccc
     6601 ttaccttaca caacatcatc tcttcaacaa gatgctgcta ataaaatcaa tttcagaacg
     6661 cgtaaaacca tgatggtagc ccaacaactg tatgagggga ttcatttagg agaaaatgga
     6721 actcaaggtt taattaccta tatgcgtacg gattctactc gtatcagtcc agtagcacaa
     6781 aacgatgctg ctcaatttat tatcaatcgc tttggagcta attattcaaa acatggtaat
     6841 cgtgtcaaaa ataccagtgg tgcccaagat gctcacgagg cgattcgtcc atctagtgtt
     6901 aatcatacgc cagactctat tgccaaatac cttaacaagg atcagttaaa actttatacg
     6961 cttatttgga atcgttttgt ggctagccaa atgacagcag ctgtttttga taccgtaaag
     7021 gtgaacttgg aacaaaatgg cgtgattttt gttgccaatg gtagccaaat gaagtttgat
     7081 ggttacatgg ctgtttacaa tgattccgat aaaaacaaaa agttacctga gatggcagaa
     7141 ggagaaactg ttaaaaaaat atcgacctct ccagaacagc actttacgca gccaccagct
     7201 cgttattctg aagcaactct gattaaaacg ctagaagaaa atggtgttgg acgtccatca
     7261 acttacgctc caaccttaga agtcattcaa cgcagatact atgttaaatt gtctgctaag
     7321 cgctttgagc caactgagct tggggaaatt gtcaacaagc ttattgtcga atttttccca
     7381 gatattgttg atgttgcctt tactgccgag atggaaggta agctagatca agttgaaatc
     7441 ggagaagaac aatggcaaca tgttatagat caattctatc aaccctttgt caaagaattg
     7501 aataaggctg agtctgaaat cgaaaagatt caaatcaaag atgaaccagc tggtttcgac
     7561 tgtgatgttt gtggtcaccc aatggtcatt aagttaggac gttttgggaa attttacgcg
     7621 tgtagcaact tcccagagtg ccgtaataca aaagcaatca ctaaagaaat tggtgttact
     7681 tgtccggttt gtcataaagg acaagttatc gagcgtaaaa ccaaaaaaaa taggatattt
     7741 tatggttgtg atcagtatcc agattgtgag tttatctcgt gggatttacc tattggacgt
     7801 gcttgcccta agtcaggtga ctatcttatt gagaaaaaag ttcgtggtgg caaacaagta
     7861 atgtgtagca atgagacctg tgattacaag gaagaaaaaa ttaaataaaa aaagaattgg
     7921 aactccttgt cttgttccaa ttcttttttt tatttacaaa ggtattttta actacaaaaa
     7981 tcgctagttc gtttttaaaa ggcttaaaaa ttcctcaata actggactga aactcgcatc
     8041 atctcggtaa gcaatcccaa tagggtaaaa attattttta tcttgcaaag gaatccaaac
     8101 taagttatct acctgattaa gactctcaaa atcactcggg aggatagtca aggcattcaa
     8161 atcgtgcaaa ctatgaataa gaacttccca atcattgtgt ttaaagacaa tatctgattc
     8221 aaaaccgaga gctcggcatt ttctaggaag catttcgcct agcatgtagt gatcgtttaa
     8281 agaagcaatc ttatagcctt ttaaatcttt aagttttaac tgaggatgtt gcgccaaagg
     8341 gtgctgtttg aggagaacga tactgacctt atagcctttt gtggacgttt gtaatagctc
     8401 aatggtaata tcctttcgaa tacttagaaa ggagaggaga tcaatatcga tttttccttg
     8461 ggacaataat tcttataatt tgcgagaacc atcctgtata agcgataatt caacatttga
     8521 gtgagtagcc ataaagattg aaatttgttt catgaattgt atagcaaaca gtgatgttaa
     8581 ttcaacacga atcatctctt tttttcttga tttaattgct gaatatccgc aacaatttga
     8641 ttaaagtcat ccacaaggtc tttgcctttg tgataaagaa tatctccagc ttcggttagt
     8701 agtagctgtc aatcactttg tgtaaaaagc gtcgtgttca aactaggact ctagcctttt
     8761 aattgattga gaaagagtag gttgcgttac aaacaaactt ttagcagcgt gggaatgatt
     8821 ttttgtttca gcaactgcaa taaaataggt taattgtcta gtgtccatag tagatccttc
     8881 atgattggtc aaggaatagc gtctctactt ggtttacaac ctattttgat gtctctcctt
     8941 attgccatga ttttctgctt ccttattgtt tctccaatta caacggtagg aattgctctt
     9001 gcgattaact tatctggtat tggttcaggt gctgcaaact taggtatttg tgccgcaagc
     9061 tttggacttt gcttagcagg ttgggctgtt aattcaaaag gaacttcact tgctcacgtc
     9121 ttgggatcac cgaaaatttc aatggccaac gttttatcaa aaccaaaaat catgttgcca
     9181 atgttatgtt ctgcagctgt acttggtgtt attggtgcta tctttaatat tcaaggaaca
     9241 cctgctagtg caggctttgg gattagtggt ttgatcggac ctatcaatgc ccttaattta
     9301 gctaaaggcg gatggtctct agtaaatatt ctacttatca ttatcatttt tgtaggggca
     9361 ccaattgttc tcaatataat ctttaaccac ctttttatca aggtgcttaa agtgattgat
     9421 ccaatggatt acaaacttga tatctaagaa aggaactttt gatgaaatta aaattatata
     9481 atgttagagg cgaagaagca gtcttagcaa aaaaatgggc tgatgacaat gggattgaga
     9541 tttccttgac agagtcttcc ttaactcctg aaacggttaa agaagctgaa ggctttgacg
     9601 ggattgccaa tgcccaaatt ggtcctttag atgatgcgat ttatcctctc ttaaaggaaa
     9661 tggggattaa acaaatcgct cagcgcagtg ctggtgtcga tatgtataac cttgatttgg
     9721 ccaccgaaaa tgacattatt atcacaaatg ttcctagcta ttctccagaa tccattgcag
     9781 aatttacggt tactattgcc cttaatttaa ttcgtcatgt ggagttgatt cgtgaaaatg
     9841 ttaaaaaaca aaatttcact tggggactcc ctatccgcgg ccgtgtttta ggtgatatga
     9901 cagttgcgat tatcggaact ggacgtattg gtctagctac tgctaaaatc tttaaaggtt
     9961 ttggcttcaa ggtcgttgga tatgatattt accaaagcga tgctgccaaa gctgtcttgg
    10021 actataagga atctgtagaa gaagcgatta aagatgctga tcttgtttcc ttacacatgc
    10081 caccaactgc agagaacaca caccttttca atgctgattt atttaaatca ttcaaaaaag
    10141 gggctatttt gatgaatatg gcgcgtggtg ctctcattga gacgcaagat ttactggatg
    10201 ctttggatgc aggcttactg agcggagctg gtattgacac ttacgaattt gaaggacctt
    10261 atatacctaa gaattttgaa ggtcaagaaa ttacagattc actatttaaa gctttgatta
    10321 accatcctaa ggttatttat acgcctcatg cagcttatta tactgatgaa gccgttaaaa
    10381 acctagttga aggtgccctt aatgctacag tagagattat caaaactggg acaacgacaa
    10441 ctcgtgttaa ctaacaactg atttgacgtc cattataaag gaataatttc aaaaattcct
    10501 ttataatgga cttttttgca ttagtccgtt ataatagact gtaagaaagg ggactcttat
    10561 gacatatata gcgcttattg gagatattat ccagtccaaa caattgacgg atcgttccaa
    10621 agttcaaaaa actttagcag cctatttaga cgacctcaac aaaacctttg ctccttatat
    10681 tatttctaaa ttatccctaa cgttgggaga tgaatttcaa ggactttttc aggtggatac
    10741 tcctattttc catctgattg atttgattaa tcatcacatg gatatcccta ttcgatttgg
    10801 agtaggagta ggttctattc taacagacat caatcctgat attagtattg gagctgatgg
    10861 acctgcttat tggcatgctc gtgaggctat tcgttatatt catcaaaaaa atgattatgg
    10921 taatactaca ttagctcttc ggactggtca tcacaaccag gatgatgtat taaacagttt
    10981 actagcagct ggagatgcta ttaaagccag ctggcgagct agtcaatggg aaatttttga
    11041 cacgctttta gatttgggca tttatgagga atactttgat caacaacgac taggaaagca
    11101 attatctctt agctctagcg ctttgtcaaa acgtctaaaa agtagtcatg ttaaaattta
    11161 tctgagaaca cggcagagtg cgctaaactg tctcaaacaa atcaaggagg aagctgatga
    11221 ttaatggtgt ctctcactac ctagctcaaa cccctacact taccctgttt ctcatttgtc
    11281 atgtgttatc agactaccag ttacaaagtc agcaagtagc tgatttaaaa gaaaaacatg
    11341 tgacttattt aggttaccac ttgattggag ttagcattcc cctaatttgt ttaaccctca
    11401 ttatcccaca agcatggttg atgagcctat tggttatgat aagtcacgcg cttattgact
    11461 ggctaaagcc aaagatggct aactctctaa aatggaaaag agagtggatt tttctgctgg
    11521 atcaatgctt acatatcgct attagttcat ttgctgggct aaggttagca ggggtgacct
    11581 tgcctaattg gcttccgata tctattttga tgaccgtttt atttatccta ctcattacca
    11641 aaccaactaa tattgtcttt aaactctttt tcagcaaata ccagcctgac caaggagaaa
    11701 aaatggacac gatcatagga gcggaggcta ccataggaat tctagagaga attgttattg
    11761 gggtgtgtat gatcatgggg caatttgctt caataggtct ggtctttacc gctaaatcta
    11821 ttgctcgcta taataaaatt tcggaaagtc cagcttttgc agaatactac ctaattggtt
    11881 ctttatttag tattctcagt gtttttatcg cagcatggat ttgtttcttc tagttgaaaa
    11941 gggttcccgt agaaagaggc taacgcttag tctctttctt tttctttctt cttatttctg
    12001 ttataataag tcaggtaact acattagaaa gatttgtggg tctgtcatct aagagatgtc
    12061 attcccgaag aggtattagt gacttgtctc aatcaactgc aacttatatt aatgttattg
    12121 gagctggact agctggttct gaagctgcct atcagattgc taagcgcggt atccccgtta
    12181 aattgtatga aatgcgtggt gtcaaagcaa caccgcaaca taaaaccact aattttgccg
    12241 aattggtctg ttccaactca tttcgtggtg atagcttaac caatgcagtc ggtcttctca
    12301 aagaagaaat gcggcgatta gactccatta tcatgcgtaa tggtgaagct aatcgtgtac
    12361 ctgctggggg agcaatggct gttgaccgtg aggggtatgc agagagtgtc actgcagagt
    12421 tggaaaatca tcctctcatt gaggtcattc gtgatgaaat cacagaaatc cctgacgatg
    12481 ccattacggt tatcgcgacg ggtccattga cttcagatgc cttggcagaa aaaattcacg
    12541 cgctaaatgg tggcgacgga ttctattttt acgatgcagc agcgcctatc attgataaat
    12601 ctaccattga tatgagcaag gtttacctta aatctcgcta cgataaaggc gaagctgctt
    12661 acctcaactg ccctatgacc aaggaagaat tcatggcttt ccatgatgct ctgacaaccg
    12721 cagaagaagc accgctgaat gcctttgaaa aagaaaagta ttttgaaggc tgtatgccga
    12781 ttgaagttat ggctaaacgt ggcattaaaa ccatgcttta tggacctatg aaacccgttg
    12841 gattggaata tccagatgac tatacaggtc ctcgcgatgg agaatttaaa acgccatatg
    12901 ctgttgtgca attgcgtcaa gataatgcag ctggaagcct ttataatatc gttggtttcc
    12961 aaacccatct caaatggggt gagcaaaaac gcgttttcca aatgattcca gggcttgaaa
    13021 atgctgagtt tgtccgctac ggcgtcatgc atcgcaattc ctatatggat tcaccaaatc
    13081 ttttaacgga aaccttccaa tctcggagca atccaaacct tttctttgca ggtcagatga
    13141 ctggagttga aggttatgtc gaatcagctg cttcaggttt agtagcagga atcaatgctg
    13201 ctcgtttgtt caaaagagaa gaagcactta tttttcctca gacaacagct attgggagtt
    13261 tgcctcatta tgtgactcat gccgacagta agcatttcca accaatgaac gtcaactttg
    13321 gcatcatcaa agagttagaa ggcccacgca ttcgtgacaa aaaagaacgt tatgcagcta
    13381 ttgctagtcg tgctttggca gatttagaca cctgcttagc gtcgctttaa cactaaaaaa
    13441 gagttccttt tcagttgagt tgctgaagag gactttttta tttgctgatt tgagcgctag
    13501 ctatgcaaaa gaatgcacaa aaaatacatg aatagactat ggtcactaga agcaatatta
    13561 tataatgaat agtactcatt agttgattca gtttggaggt tttagcatgc agcagcaagt
    13621 cgcaataaca gaaacagttt tacgggacgg tcatcaaagt ttgatggcaa cgcgtttatc
    13681 tattgaggat atgcttcctg tccttaccat cttagacaag attggttact attccctaga
    13741 gtgttgggga ggagcaacct ttgatgcctg tattcgtttt ttgaacgaag acccttggga
    13801 aaggctgcgc acgttaaaaa aaggcttacc taatacccgt ttacaaatgt tattaagagg
    13861 ccaaaacctt ttaggctacc gtcattatgc agatgacatc gttgataaat tcattagttt
    13921 atcagctcaa aatgggattg atgttttccg catttttgat gccctaaatg acccacgaaa
    13981 tatccagcaa gccttaagag ctgtgaaaaa gacggggaaa gaggctcaac tgtgtattgc
    14041 ctatacaact agcccagtgc acacgcttaa ctattacctt tctttagtga aagaattggt
    14101 tgagatggga gctgattcta tctgcatcaa agatatggct ggtattctaa ctcccaaagc
    14161 ggctaaagag ttggtatctg gtatcaaagc catgactaac ttacctctta ttgtgcatac
    14221 ccatgcaact agtggcattt ctcagatgac ctatctagca gcagtagaag ctggggctga
    14281 tagaattgat accgctttgt caccttttag cgaaggcact tcacaacctg ctacagaatc
    14341 tatgtattta gccttaaaag aagcaggtta tgatatcaca ttagatgaaa cgctactgga
    14401 acaagctgct aatcaccttc gtcaagcccg ccaaaagtat ttagctgatg gaactctgga
    14461 cccaagtctt ctctttccag acccaagaac gctacaatat caagttcctg gaggcatgtt
    14521 atctaatatg ctatctcaac tgaaacaagc taatgccgaa tcaaaattag aagatgtttt
    14581 agcggaagtt cctcgggtaa gaaaagactt aggttaccca ccgttagtaa cgccacttag
    14641 tcaaatggtc gggactcaag ctgctatgaa tgtgatttta gggaaacctt atcaaatggt
    14701 gtccaaagaa atcaaacaat atttagcagg agaatatggg aagacacctg cacctgtcaa
    14761 tgaagacctg caaaagagtc aaattggatc aacaacacta accactaatc gtcctgcaga
    14821 ccagttgaat cctgaatttg aagtattgaa ggcagaaata gcagatttag cccaaacgga
    14881 cgaagatgtg ctgacctatg ctctcttccc aagcgtagca aaaacatttt taacaaccaa
    14941 ataccaaact gatgacgtca ttaaagtgac tgcctttatc aaagcatagt aggctatctt
    15001 aatcagatag aaacaaaaat atggagatca aatgacctat tcctttattg atatcttaca
    15061 agttacctta gtatccttgt tcttagtgtt aacctgttta acagggatta tgggattaat
    15121 gatgctaagt ggtaaattat tgtcacaaga aaaacctctt ctccctcaag cctctcctaa
    15181 gagtcttcaa gacaaagaag ctacccaagc gagtggagaa gctctcgaaa cccttttggc
    15241 taaggatgaa ttagctagga ttgctgttat gactagtctt gcttatgcta gccaagaaga
    15301 gtcaggaaaa cgatttgaag ttgttgctgt ggaaaggaaa tcctaatgaa aagatatgaa
    15361 gtgactgtca atggacaggt ttatgaagtg agtttacgtg aactagctga tggagaaact
    15421 gttgaagtta gccagtcagc agcacctgct actgagaaag aaatgaatac caacgctgct
    15481 ggcggtggcc tacaggtaaa ggctccaatg tctggaacag tcttgtccat ctttgctaca
    15541 gaaggaaaag ctgttaaaaa aggagaagct gttctggtct tagaggccat gaaaatggag
    15601 aatgaaattc tagcacctgc agatggtctg gtttcaaaaa ttcatgttgt ggctaaccaa
    15661 acggtcgagt ctgaacaagt tttgatttca ttctaggagg aaaccatgct agacgtactc
    15721 aatcaaatgg tccaatcgtc aggattggct catctaactg ttaataatct tatcatgatt
    15781 tgcttggcct gtttcttttt ataccttggt atcaaaaagg aatatgagcc ttaccttatg
    15841 gtaccaatcg cctttgggat tttactcgtt aatttgccta tggcgggatt gatggaccac
    15901 cctgccaatg gtaatcctgg tggccttctt tattacctat ataagggaac tagcctaggg
    15961 atttacccac ctttaatttt tctttgtttg ggggcatcaa ctgactttgg tcccttgatt
    16021 gccaatccta aaaccatttt actaggaggc gcagcacaag ttgggatttt cttagccttt
    16081 ttccttgcta ttatgttagg catgacacct caagaagcgg cttctgtcgg gattattggt
    16141 ggtgccgatg gaccaacagc tatttatgtg acgacaaagt tggcgcctga tttactctct
    16201 actatcgccc tagcagctta ttcttacatg gctctggtac ctattattca acctcccatc
    16261 attaaactct tgactaccaa ggcggaacgc caagtgaaaa tgactcaagc aagaactgtt
    16321 tcacaaaaag aaaaaatcat tttcccgatt atggtaacaa tctttgtcag ccttttggtt
    16381 ccaagtgcta caaccttggt tggctgtttg atgctgggaa atctggtcag agaaattaaa
    16441 attgttccta aaattgtaga gaatctccaa caagtggtta tgttttgcat tactatcatt
    16501 ttagggttaa ccgttggtgc taaggccaat ggagatctct ttttatcagc cacaaccttg
    16561 aaaattattg cccttgggct aatcgccttt gctgctggga cagcaggggg tgtcttgatg
    16621 gggaaaatca tgtactattt atctggtaac aaagtgaacc ctatgatagg agcagcaggt
    16681 gtttcagctg ttccgatggc agcacgagtg gtccaaaagg tcggacaaga agaagatcca
    16741 agtaatttct tactcatgca cgccatgggg cccaatgtag ctggcgttat tggctcagcc
    16801 attgcctcag gtgccttgct ggccttcttt ggctaattgt gaaaaaggat tagtaaatta
    16861 atcttaataa atcattatca ggagttggaa gcaatccagc tcctttttta gtagaaagct
    16921 cgagtgataa actattccaa aataattata aaaaaagact gtcctgattg tacggactat
    16981 atcagttgac ttttatagtc tagctgaaat agcagtgcaa aactaagaca ctcgttttaa
    17041 agtagttttt ctaaaaaagc aaaataaaag gtcagagcca ataaatctgc cgcgccccca
    17101 gggcttaaat gctgattaat gaggcattga ttgtaggagg acaaagccaa tcttaggtca
    17161 gttgttgata aatcttgttg caggagtagt cgcatatctg ccttaacgcc tttccatgct
    17221 tcaatccctc cacgatgaat cagatttcca tcttcaacaa tagacatcaa ataaacaaga
    17281 agacgaagct gactggtttc ggggtcattt tgacttatca tctgacgaaa gtagggcaag
    17341 gcatggtctg ttaaggtcgt gaatccctcg cttgcctcac cccgaggtcc tttgatgcca
    17401 taagttacaa aaagttgctc cccataagtg aactcttttt tagtgttcaa atgaccaagg
    17461 tctgtttgaa tcaaatgtgc cgtcataggt tttactaaac gacagatagc tagagtatct
    17521 tccttgctaa aacgccctaa atcagtcatt aagtgaggtg ttcttgctag ataggcccca
    17581 gttgcaccaa gaagtaaagc catggaaaaa ttcaagcctt tatgagtgtt aattccctga
    17641 gtagcacaaa acatggtttc ctcggccttt tgacctagtt gtctaagacg gttaaacaga
    17701 agaagtggct cttcttttgc ataagcaaaa cctgtttcga tataagcctg aaaaaaagga
    17761 gagagtgcta tcatactgtc gataaaggtc ataaaggaca tgtcatcatg ggcaccgtta
    17821 tcaaatcgat caactaaacc aggttttgga gatagtgaga cttcataaag caaagctttt
    17881 aaggccagtt gactaatact tgttagaact gccttagtca ttttgctcag gcctcatttc
    17941 ttttaagata aattcaagtg agctattcaa gtgttcatgt gtaatcaacg taatttgctc
    18001 atgatctttg ttcttcatac ctcggtaaat catccagtgt tcagatagag ctcgtttacg
    18061 gcgttcaact gaagaaatcg aaatttttct aaagtagacc aaataagttt gcaattctgt
    18121 cacaatttct tttaagcgga ccatttggct atagctgtaa atcaaattgt taaaggcatt
    18181 aaaattatcc aaaatacgat tggtgtcatc ctctgcgata aaggtttcac agtcttccaa
    18241 caagttatgc atgactttaa aatcttcttc tgtcattaaa tgcatcgctt gcactgttgc
    18301 gagagtctct agagcttttc gaatttcaaa aatttcacag gcatccttga tactaactcc
    18361 tttgacaata atgccttttt taggaatgtg ctctacaagg tgttcttcag acaataatcc
    18421 caaagcgtag cgaataggtg tcctgctaat attaagcgca atggaaaact ctttttcatt
    18481 aatgcggctt ccaacaggaa tttgtcgcaa aataatggtc tttttaagag cgttataaaa
    18541 ggctattttc aaagggatat ttcttgatag gtcaagattt tgtttaacgg cttcgataat
    18601 aggattcatg tcatctcctt aatgctagtg tcctcacttt attatactta aatttgtgaa
    18661 acattccaat aggactagat gagttctgaa aaattaaagc catgctcaca aaaggacatg
    18721 actttaatca tctcataagg aataaggtag tagtgttatc gttggtttat tgtggaatgt
    18781 aaagtggcat ttgccccatg gcaataatgg ttaccacaaa gataacaaag atgataaggg
    18841 catatttagc agcctctttt tgccattctc ccatatcaag acctgtcaag cgaagaagga
    18901 ggtaaatgaa ggcaactaat ggactaagta agtggaaggc ttgtcccatg agggaagcaa
    18961 gtgccatagc catgttactg aaaccatatt cagcaccagc ttctgcaaga acaggcaaga
    19021 taccatagta aaaaccatca ttggagataa agaaggtacc aggtgcagaa actaaggcaa
    19081 taacgagacc ccagaaacct gccagttgtt ttggaataat ggttgcaaaa ctttgtgcaa
    19141 gagcgctagc cataccagaa ccttggaaaa gacccataaa gataccagca gcaaagacta
    19201 agataaccac ttgaaccgca tcaccagcat tatcaccaat gcgttttgat tgatctttaa
    19261 gcactgggta attgaccatc aaggcgatac aagttcccac cgcaaaaaga agaagcggtg
    19321 ccatagcaat agatttgttg aaagagccag caactaacca agtgattaat ccaatggtta
    19381 agatagcatt gaaaacaaag tttttaggac gacgaataaa aagggtatct gggtcagtaa
    19441 tgtctgtgag ttgacgcatg tcttcttcag acaagtcaat aacaccaagt cttgcacgtt
    19501 ctttacgccc catgcttgga gcaacccaac aaatcacata aagaagagat aaaatcatac
    19561 ccggtgcaag atagccaaga atttcaggac cgacaccaag aacagacatc gcacgagcag
    19621 tagggccacc ccatggcagt aagttcataa tagtattttg aagaataatc aagacaccta
    19681 ggttcataat tttcatgtcc aattttttat agataggtaa gaaagcagag caacaaatta
    19741 aagtagtggt tgttccatca ccattaagag atactgcggc agcaacgaca gctgttgcga
    19801 taagcacctt cattggatca cctttagcaa agcgaatcat cttttcagta atcggatcaa
    19861 acaatcctgc atctaacatg acagagaaga acaaaatggc aaatagcaac atgatacctg
    19921 ttttagcagt attgttgata ccatacataa ccattggtcc gatagctgtt aggtctttag
    19981 caattccacc gtcaccaaca aaggcaacaa atttggcatc tgcattgaag tcagctgaac
    20041 cagtcagtat aacagcaatc gtcatgatta atggaatcat gaccaaagca gtaaaagggg
    20101 tcattttttt cttcattacc acgtacataa agacaataat catggcataa gccagcattg
    20161 ttaataacat cattgttatc tcctttttca agttatgatt tcagtataga tgaaagcgct
    20221 tat
//
LOCUS       DAUELZ010000024        20115 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.024, whole genome shotgun sequence.
ACCESSION   DAUELZ010000024 DAUELZ010000000
VERSION     DAUELZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20115
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.024"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(156..662)
                     /locus_tag="VHI09_000667"
     CDS             complement(156..662)
                     /locus_tag="VHI09_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEQ6120561.1"
                     /translation="MKIAIIGHSGSGKSTLARFLGQHYHCEVFHLDQLHFSSNWQERS
                     DHDMIADLSTCLLKQDWIIEGNYANCLYEERMSEADYIIYVNFSRFHCVYRAFKRYLN
                     YRGKTRPDMADNCQEKFDVAFVKWILLDGRSRNQLKKYQSVVQKYSHKTIVLTNQKQL
                     SHYMNTIN"
     gene            complement(717..1157)
                     /locus_tag="VHI09_000668"
     CDS             complement(717..1157)
                     /locus_tag="VHI09_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ6120562.1"
                     /translation="MKIIEYKGNYRDDLIFMVLEAKNALRRIPSINPDLLTIETSYLE
                     KGDCFWLAVNEESRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYL
                     RKIGKSHVRIHLGQPKEQWFESYRFYPKQGYQFYDDTHLIKKLI"
     gene            complement(1298..1370)
                     /locus_tag="VHI09_000669"
     tRNA            complement(1298..1370)
                     /locus_tag="VHI09_000669"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1335..1337),aa:Lys,seq:ctt)
     gene            complement(1371..1567)
                     /gene="ssrS"
                     /locus_tag="VHI09_000670"
     ncRNA           complement(1371..1567)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VHI09_000670"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1656..2924)
                     /locus_tag="VHI09_000671"
     CDS             complement(1656..2924)
                     /locus_tag="VHI09_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEQ6120563.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATIDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDSSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLLD"
     gene            complement(2957..3523)
                     /locus_tag="VHI09_000672"
     CDS             complement(2957..3523)
                     /locus_tag="VHI09_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEQ6120564.1"
                     /translation="MILLIDNYDSFTYNLSQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LVFSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGTLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            complement(3556..5313)
                     /gene="pabB"
                     /locus_tag="VHI09_000673"
     CDS             complement(3556..5313)
                     /gene="pabB"
                     /locus_tag="VHI09_000673"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEQ6120565.1"
                     /translation="MHRKTIIDFKELGHRFLFEKTLLELVAKSLDQVGPVIQQVQHYQ
                     ELGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKEDLPLDYDSITIP
                     NQWVSATQKEAYQKAIETIHHEMRQGNTYQVNYTLQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDDFAVISASPELFFKQEGNQLITRPMKGTTKRGVNTWLDQQEHDWLQTD
                     RKNRSENMMIVDLLRNDMGKICQTGSVCVDRLCEVEQYSTVWQMTSTIVGDLKADCDL
                     IDILTALFPCGSITGAPKVSTMAIITSLEPNPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVHQKTTFLYRHKQIFDLKTTAKVEHQKIAFL
                     EQHLNRLKEAATYFAYPYDEKALQKQLSTYLENKNNAAYRLMIRLSKDGKISLSDQPL
                     EPLSADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQLFYNQAGQLLETSIGN
                     LFIQLGRTLYTPPVAVGVLPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLELTHLDALLAKSQA"
     gene            complement(5498..7471)
                     /locus_tag="VHI09_000674"
     CDS             complement(5498..7471)
                     /locus_tag="VHI09_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEQ6120566.1"
                     /translation="MRYSRRYPSQTRQMLLALLLMTASLQAGAMTTYADEVNTNHQPL
                     TAQVEASKPTAMESVTSPADQTHPISTQEASSPLHPLTTEATPAAQESPITLEDYKAA
                     SASQLAEWARQQRVTGQQLLDFALETIKETNPELNNVISLREPLARQESEQMTDEGQP
                     FYKVPILVKGLGHTVAGSSNTNGLAFLKDKTSSSTSAFVKQLQKAGFIVVGQSSFPEM
                     GWINVTNSNLYGNTHNPWQLDQNPGGSSGGSAAAVASGQVSLASASDGGGSTRIPASW
                     SGLIGLHPTRGILEGNPTSERSNVSHFALTKSMEDTEKLFQFLLKDKAKAQQNPQRLD
                     TSIPIAYSTQTPAGTPISEEAIAAIKEAVAFLQEQGYQTVEVPYPVDGKLMMQYYYTI
                     AASAAPSINFMAQQTLKRPLQKEDVELLSWALYQTGKDLTKEDVNKAWEGIAAMTEQL
                     NQFYQKYPIFLTPTTAYPAPAADYHHIPKDLVAQLSDMSGLSKEEKLDLIYRQWLPAW
                     TLTPFTQLANLTGTPSLSLPTHVTKSGLPLGILVNSGAHNDSLLLQLGQLFEKANRFH
                     ILTAGKKGLPETPAHEHNLSTQSENKQGVAIPVTYQTKGFTTGPTKGQNGLVTLPQTG
                     DGQSKGVLLTSYISLFLGTLFLAGSFWSNKVKD"
     gene            7644..8114
                     /locus_tag="VHI09_000675"
     CDS             7644..8114
                     /locus_tag="VHI09_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEQ6120567.1"
                     /translation="MAKYGFLSILEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKVDFGLEWNAQAFEEGQQGLEEAASYPYPRY"
     gene            8185..9138
                     /locus_tag="VHI09_000676"
     CDS             8185..9138
                     /locus_tag="VHI09_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEQ6120568.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIGQKD
                     RFGELYPDVEQSDMIAITAYYPSSTNLADVTVTINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQILRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQENIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLVKDQGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMIQGEWNALVFKKTDDISGVIGG"
     gene            9138..9887
                     /locus_tag="VHI09_000677"
     CDS             9138..9887
                     /locus_tag="VHI09_000677"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEQ6120569.1"
                     /translation="MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLADEAEVILVFDDG
                     VKYLAKVTNSMAHELEIIEALPDQVELPVEVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEHKAEFLKSLSS
                     FDHIFIGYEETAKAGQLATLAREVKEVKPGAKILFIFGPEGGISPAEITQFEAAGVIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            10245..12431
                     /locus_tag="VHI09_000678"
     CDS             10245..12431
                     /locus_tag="VHI09_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ6120570.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHDSAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGAFASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYLVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSILFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDHNTQARPTQVADSNSQVVQIINLLGGAGNIDDVDACMTRLRVTVKDP
                     AKIGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGAVIPEVNMSQLT
                     SKPTPAKDFKHVTEKVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTNGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETTIVVAFTNTAEIQDVTLTSLGAQPAKTKVATVEL"
     gene            12514..13332
                     /locus_tag="VHI09_000679"
     CDS             12514..13332
                     /locus_tag="VHI09_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEQ6120571.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPSIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMADETNYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLDLQDSHKVADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLDWR"
     gene            complement(13727..14209)
                     /gene="nrdI"
                     /locus_tag="VHI09_000680"
     CDS             complement(13727..14209)
                     /gene="nrdI"
                     /locus_tag="VHI09_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ6120572.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEMEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNDTQTLPNS
                     "
     gene            14590..15696
                     /locus_tag="VHI09_000681"
     CDS             14590..15696
                     /locus_tag="VHI09_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ6120573.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEGMTSGE
                     RTQKINEIKEFIKKIPKTDQEQFRHRPWYGGDYFKDEDYQKELNKFTEDALKKIVDLI
                     EKGATKGEKGDAGPRGERGPQGPVGPAGKAGAKGDKGEAGAPGKDGAQGPKGDKGETG
                     ERGEKGEAGIQGPQGEAGKDGAPGKDGAPGEKGEKGDRGETGAQGPVGPQGEKGETGA
                     QGPAGPQGEAGKPGEQGPAGPQGEAGKPGEKAPEKSPEVTPTPEMPEQPGEKAPEKSP
                     EVTPTPEMPEQPGEKAPEKSKEVTPAPEKPADKEANQTPERRNGNMAKTPVANNHRRL
                     PATGEQANPFFTAAAVAVMTTAGVLAVTKRKENN"
     gene            15948..18167
                     /locus_tag="VHI09_000682"
     CDS             15948..18167
                     /locus_tag="VHI09_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEQ6120574.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKADVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGQDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLTHLTTVVEKIKAVPDVYSVKRTNG"
     gene            18182..18625
                     /locus_tag="VHI09_000683"
     CDS             18182..18625
                     /locus_tag="VHI09_000683"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEQ6120575.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     SQLYDSFNEQLAEFVPVERGVFGADMQVSLINDGPVTIILDTKCH"
     gene            complement(18723..20045)
                     /gene="ska"
                     /locus_tag="VHI09_000684"
     CDS             complement(18723..20045)
                     /gene="ska"
                     /locus_tag="VHI09_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEQ6120576.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGPEWLLDRPSVNNIQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSGPAQGGKTEQGLSPKSKPFATNKGAMPHKLEK
                     ADLLKAIQEQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADRDDSVTLPTQPVQE
                     FLLSGHVRVRPYQPKAVHNSAERVNVNYEVSFVSETGDLDFTPLLRNQYQLTTLAVGD
                     SLSSQELAAIAQFILSKKYPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQ
                     AYKANSKTGIVEKTNNTDLISEKYYVLKKGEKPYDPFDRSHLKLFTINYVDVNTNELL
                     KSEQLLTASERNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHNDTNRIIT
                     VYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPNDK"
BASE COUNT         5765 a         3971 c         4761 g         5618 t
ORIGIN      
        1 tgactatcaa atgaaaattg agacatgata ctaaaagtgg attgacgctt ttaattaagc
       61 cgattagtta tgaaacaatc aaaaaagcct ttaaacaaag gctttcagtc tgaatagccc
      121 ggattatccg tggttatttt agttttgcac tctaattagt tgatcgtatt catataatga
      181 cttagctgct tttgatttgt taagacaatg gttttatgag agtatttttg gacaacggat
      241 tggtattttt ttaattggtt tcttgaacgt ccatctagta gaatccattt cacaaacgca
      301 acgtcaaatt tttcctgaca gttgtcagcc atatcaggac gcgtttttcc cctatagttt
      361 aaataacgct taaatgctcg atagacacaa tgaaaccgac tgaaattaac atagataatg
      421 taatcagctt cactcatgcg ctcttcatag agacaattgg cataattccc ctcaataatc
      481 caatcttgtt tgagcaggca tgttgataaa tcagctatca tgtcatgatc tgaccgttct
      541 tgccagttcg atgagaagtg tagttggtct aaatggaata cctcacaatg gtaatgttgg
      601 cctaaaaatc gtgctaaggt tgactttcca gagccactat gtccaatgat ggcaattttc
      661 aatggatacc tcctaaaaat gacgtatgaa atgactataa agggtatgaa atgtgtttag
      721 attagtttct tgatgagatg cgtgtcatca taaaattgat aaccctgttt aggataaaac
      781 cgataagact caaaccattg ttctttaggt tgacctaagt ggatacgaac atgactcttg
      841 ccgattttcc ttagataatc ttctgcaaac gtcagaagtt gactgccaat gccttgatgt
      901 tttaagcttg gtttgatgaa gagacggtgc aagaagacct catttgtttt gggaatagaa
      961 gaataagcga ttgagccaat aacacgactt tcttcattga ccgctagcca aaaacaatcc
     1021 cctttttcca aataacttgt ttcaattgtc aaaagatccg ggttaatgct gggaattctc
     1081 cttaaggcat tttttgcttc caagaccata aaaatgaggt catctctgta atttcctttg
     1141 tactcaatta ttttcataac agtctccacc ttaatcttaa gcgctttaat tggtatcata
     1201 ccataaaaaa attactgcgg ggataaatga tggaacaaat cctcttctga caagcaaaaa
     1261 aagcctttaa acaaaggttt tttcatgtat agttgtatac cccctacagg gctcgaacct
     1321 gtgacccata gattaagagt ctactgctct accaactgag ctaaggaggc aagaatgctg
     1381 tattggcacc ggtgatccac gtttttgtat tgagcccgcg cagtataagc aggtgggtaa
     1441 cgcgctcttt cttggaagtt gcgtccgcgt gaaacggctt gcagactaga aagagtcttt
     1501 gtttcccgaa aacacaaaga aatagtcggt caacacttag atgtggattc gtatgccaca
     1561 gcaatgtttc tgtactttta taataccact ttttaaaatt ttttcaagca aaattatcga
     1621 tttttgaaat cggttgcgat ttttaaaact ggcagtcagt ctaagagctt atcaatacgc
     1681 tctttatttg aaccaagtgc gcgttcgtat ttaccggttt cattggcttc aaaataatga
     1741 tgtcctacta atttatctgg taggtattgt tgtttgaccc atttttcagg gtaggcgtgt
     1801 gggtagaggt aatctttggc attgcctagg tccttgctgc cagcgtaatg cccgtctcta
     1861 agatgccttg gaatggggag gttacctgat gttctcaaat cagctaaagc tgcatccata
     1921 gctaaataag ctgaatttga tttaggagat agagccaaat caatgaccac gttagcgata
     1981 agaatacgag cttctggaaa accaatgcgt tgagcggcat ctagtgcggt gacggtatga
     2041 acttgggcgt cagggttggc taagccaata tcttcgtaag caataatggt caatcgtctt
     2101 gctaaactag gcaagtctcc agcttctact aaacgagcgg cgtagtgcag gctagcattg
     2161 acatcagaac ctctaatgga tttttgtaag gcagataaaa catcataatg cccgtccccg
     2221 tttttatcca tggtaatata actacgctgc agactatttt ccattgtctc tagactaata
     2281 tgacggctac tatcttcatt aggagaagta gacatgactg ctagatccaa ggaattataa
     2341 gcagatcgta ggtctccatt tgtggcggta acaatgaaat ctagcgcctc atcatcaatt
     2401 gtcactaaaa aaggaaatcc ccgttctttg tcggaaattg ctagctgaat ggctgtttta
     2461 atatcctcgt tagaaagtgg ttccaattca aaaatctgga ccctgcttct gatagcgggt
     2521 gtcactgaaa agaagggatt ctcagtggtt gcgccaatca tgataatggt gccgttctca
     2581 agtaagggta aaagaaaatc ttgttttgtc ttgtcaaggc gatgaatctc gtctagtaat
     2641 aataccaagc caccagaaaa tttagcttct tccgcaattt cttggagacg ttttttgctg
     2701 tcaatggtgg cattaaaggt ccgaaaggca tatctagtag taccagcaat agcagaagca
     2761 atggaagttt taccgattcc tggagggcca tagaggatca tggacgatag tctatttgct
     2821 tccaccatgc gacggatgat tttgccttcg ccaacaaggt gtttttgtcc gatgacctct
     2881 gaaatggttt tggggcgcat tctgagcgct aaatgatcag gcatgtaact gtcctccttt
     2941 cttttggggg atgagattaa cggggaatgg ctgcgatgaa gttggcgatc atggtcatgc
     3001 catcaggagt tccgatgctt tctgggtgaa attgtagccc aaaaagtggc agggtgtggt
     3061 gttcaaaggc catgatttct tgatcgtcac agtctctagc agttacgcta aaaccttttg
     3121 gtaactgatc cacaacgata gaatggtaac gcatgacggt gatctcttgt ggcagggagc
     3181 gaaaaagact agcagggcct tgcgtttcaa tggtgctttg tctcccatgc atgacgcgtt
     3241 ttgccaagcg taaggttcct cctagagttt cagcgatagc ttggtgtccc agacacactc
     3301 ctaagatagg ttttgtttgg taaaagtctt gaatgagttt tggcatttgg ttggcttcct
     3361 tgggccaacc aggaccaggt gaaaagacta gagcgttagc ttttttggcc atgtcatata
     3421 agtttgggtc ttggttatac aagacaatcg tctcgtcaaa ttcacttaga tattgggaga
     3481 ggttgtaggt aaatgaatcg taattatcaa ttaagagtat cataggttcc ttaataacaa
     3541 aagatgagtg gcagtttagg cttgactctt agctaagagg gcatcaaggt gagtaagctc
     3601 aaggttgaga ggatagaggc cacggacagc atttcctcca aaaatagcgc tagcctcttt
     3661 taaatcagct agtgtcacct ctttttcctg agcttgacca gtagctagca gctcttggcg
     3721 aaaaagtcct ggtaaaacac ccaccgccac tggtggtgtg tagagggtcc gacccagctg
     3781 gataaaaaga ttgccgatgg acgtttctaa taattgcccc gcttggttat agaatagctg
     3841 ttcataagat ttttgctcaa tatgaggccg gtagctggtc ttaaagtagg taaaaggcga
     3901 agctgtcacg tcctttttct gtaaagagag ttgagctgtc agaaaatcgg ctgacaaagg
     3961 ttccaagggt tgatcagaga ggctaatctt tccatctttg gataaacgaa tcatcaaacg
     4021 gtaggcagca ttattcttat tttccaaata ggttgacagt tgtttctgca aagctttctc
     4081 atcgtaaggg taagcaaaat aagtggctgc ttctttaagg cgattgaggt gttgctcaag
     4141 gaaggctatt ttttggtgtt ccaccttggc ggttgttttg aggtcaaaga tttgtttgtg
     4201 ccgatagaga aaagttgttt tttgatggac ttcttcgtat tcgtcttccc acttgctttg
     4261 ccaagtgata ccgcctccga caccataagt agcttgatta tgactgagct ggatggttct
     4321 aatgggaaca ttgaagaaac ggcgaccgtc aggtaaacaa atgccaatac ttccacaata
     4381 aatccccctt gggtttggct ccagagatgt gataatcgcc atggtggaga ctttaggagc
     4441 ccctgtgata gagccacaag gaaagagagc tgtgagaatg tctatcaggt cacagtccgc
     4501 tttgaggtca ccaacaatgg ttgaggtcat ttgccagacg gtagagtatt gttccacctc
     4561 acagagccta tcgacacaaa cactgcctgt ttggcaaatt tttcccatgt cattgcggag
     4621 caagtcaaca atcatcatgt tttcagagcg atttttcctg tcagtttgga gccaatcatg
     4681 ttcttgttga tccagccagg tgttaacccc gcgcttagtg gtacccttca taggtctggt
     4741 gatgagttga ttgccttcct gtttaaagaa caattcagga ctagctgaaa taacagcaaa
     4801 gtcatcatgg gcgatatagg cattgtagcc agcagcctgt tcaacgacga gtttattgta
     4861 aatggctaag ctgtcagctg cattgagttc ctgagttaat tgaagggtgt agttaacctg
     4921 ataagtattg ccctgtcgca tctcatggtg aatggtttca atggcttttt gataggcttc
     4981 tttttgagtg gcgctgaccc attggttagg gatagtaatg ctgtcataat caagaggtag
     5041 gtcttctttc tgacaggttt tgtgcaccgt aaaataggca agatactcgt tgcctaatcg
     5101 gtcattatgg gtttgtaagg cgttgtcaaa aaaagcagct gcttcgtaac tgaggtagcc
     5161 caccacgtag taccccagtt cttggtagtg ctgaacctgt tgaatgactg ggccgacctg
     5221 atctaaggac ttggctacca actctagtaa ggttttttca aacaagaagc ggtgcccaag
     5281 ctccttgaaa tcaataatgg tttttctatg catacccaaa ttatatcata aaagcgtttg
     5341 ccaagcctaa tgagaacagg caacaactgt cttttgacca cgtaaaaaga ctgaactttc
     5401 ttagtgaaag ttcagtcttg attgactaca atatactctt atcgtaaggc aagcagcagt
     5461 gaatgtttag gtaggttaaa tctccgacga caatagccta gtcttttact ttattgctcc
     5521 agaagcttcc tgctaagaag agggttccaa ggaaaaggct gatgtagcta gttaacaaca
     5581 caccttttga ctgaccatca ccagtttgag ggagggttac taagccgttc tggcccttgg
     5641 ttggtccagt agtaaatccc ttggtttgat aagtcactgg aatggcaacg ccttgtttgt
     5701 tttcagattg agttgagagg ttatgctcat gcgcaggagt ttctggcaat ccttttttgc
     5761 cagccgtcag gatatggaaa cgatttgctt tttcaaagag ctgtcccagt tgtaacagga
     5821 gactatcgtt atgagctccg ctattcacca aaatgccaag aggtagaccg cttttcgtca
     5881 catgggttgg taaggacaag gatggtgttc ccgtcaggtt agcaagctgg gtaaatggtg
     5941 ttagcgtcca tgcaggtaac cattgccggt aaataaggtc caatttttct tctttgctca
     6001 agcctgacat gtcactgagc tgtgctacta ggtctttcgg aatgtggtgg taatcggcag
     6061 caggggcggg ataggctgtt gtcggtgtta agaagatggg gtatttttgg tagaactggt
     6121 ttaattgctc ggtcatggct gcgataccct cccaggcttt gttgacatcc tctttggtca
     6181 agtctttccc agtttggtag agcgcccatg ataggagttc gacatcctct ttttgaaggg
     6241 ggcgtttcaa ggtttgttgt gccataaaat tgatagatgg cgctgcagaa gcagcaatag
     6301 tgtaatagta ttgcatcatg agcttaccat caacggggta aggcacttct acggtttgat
     6361 aaccttgctc ctgtaaaaag gctacagctt ctttgatcgc agcaatagct tcctcactaa
     6421 ttggtgttcc agctggtgtc tgggtgctgt aagcaatggg gatacttgtg tctaaacgct
     6481 gtgggttttg ttgcgcttta gccttgtctt ttaagaggaa ttggaagagc ttttcagtgt
     6541 cttccatgct cttagttaga gcaaaatggc taacattgct acgttcagaa gtcggatttc
     6601 cctctaaaat gccacgtgtt gggtgtaggc caatcaagcc actccaagaa gctggaatgc
     6661 gggttgagcc cccaccatca gaagcactgg ctagactgac ttgtcctgaa gccacagcag
     6721 ctgcagagcc accagaggac ccgccaggat tttggtcaag ctgccatggg ttatgtgtat
     6781 ttccgtaaag attggaattc gtcacattaa tccatcccat ttcagggaaa gaggactgcc
     6841 ctaccacgat aaaaccagcc ttttggagtt gtttgacaaa ggcgctggta gatgagctgg
     6901 ttttgtcttt caaaaaggca agcccgttgg tgttgctact tccagcaacg gtgtgtccta
     6961 accctttaac cagaatagga accttgtaaa agggttgccc ctcatctgtc atttgctcgc
     7021 tctcttgtcg agccaatggt tcacgcagag aaatgacatt attgagctca gggttagttt
     7081 ctttgatggt ttccaaggca aaatctagca gctgttggcc tgtgacccgt tgctggcgag
     7141 cccactcagc taattggctc gcactggctg ccttatagtc ttctaaggta atcggagatt
     7201 cttgggcggc aggagtggcc tctgttgtta aagggtgtaa aggagacgaa gcctcttgcg
     7261 ttgagattgg atgggtctgg tctgcaggtg aagtgacaga ttccatggct gtcggcttac
     7321 tagcttccac ttgggccgtt agtggttgat ggttggtatt aacctcatcc gcataagtag
     7381 tcatagctcc agcttgtagg ctggcagtca ttaataacaa ggcaagaagc atctgtcttg
     7441 tctgagaagg gtagcgtcta gaatatctca ttctttttct cctaacatca taatcgctta
     7501 ttagtttaac ataatgggag gaaagtaagg ggtaaaacgc ttactaaaat acagaaggcc
     7561 catcagtcag catttgccag tgaaagttag ctcaaattat gctacaatgg aagcaaataa
     7621 tcgttaggaa aaaggacaat tagatggcaa aatacggctt tttatcaata ttagaagaag
     7681 aaatggataa gcattttcag tatgattatg ctatggattg ggacaagaaa aatcatgcgg
     7741 ttgaagtgac ttttgtgtta gaagctcaaa ataaagaggc cataaaaacc attgacgata
     7801 gtggcgaagt gacccaagat gatattgtct ttgaggacta tgtgctcttt tacaaccctg
     7861 ccaagtctca atttgatgca gcagattatt tggttactat tccttttgac gctaaaaagg
     7921 gcttttctcg tgaattctta gcttactttg cgcaatttct caacgatgtt gctattgaag
     7981 ggcacagcga tttgatggat ttcttagcag atgacagcaa ggttgatttc ggcttggaat
     8041 ggaatgcgca agcttttgaa gaaggtcaac aaggactaga agaagcagca agttatcctt
     8101 acccaaggta ctaaggtgac acacgagatg gtgctaacac cattaggaca ataaccaaaa
     8161 ggtcaaaata gaaaggacat acttatggaa acgtggcaag aagtgacggt tcatgtgcat
     8221 cgtgatgcgc aagaggctgt ttcacatgtg ttgattgaga caggcagtca aggggttgct
     8281 atcgcggata gcgcagacta tattgggcaa aaggaccgct ttggggagtt atacccagat
     8341 gtggagcagt ctgacatgat agctattaca gcctattacc caagtagcac taatttggcg
     8401 gatgtgaccg tgaccattaa tgaacagtta gcggagttag ctagttttgg gttacaggtt
     8461 ggtcaggtta ctgtggatag tcaggaattg gctgaggaag actgggcaga caattggaaa
     8521 aaatactatg aaccagctcg tatcacgcat gatttaacca ttgtccctag ttggacagat
     8581 tatgacgctt ctgctgggga aaaagtgatt aaattagatc ctggaatggc atttgggaca
     8641 gggacacatc ccacaactaa gatgagcctt tttgctctag aacaaatcct tcgtggtggt
     8701 gagacagtta tcgatgtagg gaccggatca ggtgtcctct cgattgccag ttccttacta
     8761 ggtgctaaaa ccatctacgc ttatgattta gatgatgtgg ctgttcgagt ggctcaagaa
     8821 aacattgatt taaaccaagg aacagacaac attcatgtgg ctgccggcga ccttttaaaa
     8881 ggggttagcc aagaagcgga tgtgattgta gccaatatct tggcggatat cttagtctta
     8941 ttgactgatg atgcttaccg tttggtcaag gaccaaggct atctcatttt atctggtatc
     9001 atttctgaga aattggatat ggttctggaa gctgccttta gcgcaggctt cttcctcgag
     9061 acacacatga ttcaaggtga gtggaatgcc cttgtcttta aaaaaacaga tgatatctca
     9121 ggagtgattg gcggctaatg caacagtatt ttattaaagg caaagcagaa aaaaaggtaa
     9181 caattaccga taaagatacc atcaagcaca tgtttcaagt gatgcgcttg gctgatgagg
     9241 ctgaagttat cttggtcttt gatgatgggg tgaagtattt agccaaggtg accaatagta
     9301 tggctcatga gttagagatt attgaggctt tgccagatca ggtggaattg cctgttgagg
     9361 tgactattgc ttctggattt cctaaaggag ataagttaga cacgattgcc cagaaagtga
     9421 ctgaattggg agccagtgcg ctatggggct acccagcaga ctggtcagtg gtcaaatggg
     9481 atggcaaaaa attagctaaa aaagaagaca agttggctaa aatcgtttta ggagcggctg
     9541 agcagagcaa acgaaatagg gtgcctgaag ttcacctttt tgaacataaa gcagagtttc
     9601 taaaaagcct ttctagcttt gatcatatct tcattggtta tgaggaaaca gctaaagcag
     9661 gtcagctagc gaccttggcg cgtgaagtaa aagaagttaa gccaggggca aaaatcctct
     9721 ttatctttgg accagaaggg ggaatttccc cagctgaaat cactcagttt gaggcagcag
     9781 gtgtcatcaa ggtgggctta ggccctcgca ttatgcgagc agagacagcg ccgctatatg
     9841 ccttaagtgc tcttagttat gctttggaat tgcaggcatc aaaataagta agctgttgct
     9901 tactttagaa agaagaggct aaagaaacga acaggcagtt cacgattgtg tcaacggcct
     9961 ttttttattg cttcttgttt ttttgaagag cgttgatggg ctcatcacca aaaaaggaaa
    10021 tgggctcttg tcctcttttt ggagagttac actactatat taatgaagaa aaatgactaa
    10081 catctgtcta agagaacgct ttacaaattg ttgcgaggct agtataataa aggtatcaga
    10141 tcaatgcaaa cgattgcatt gctgagttgt agtctatatc attgtgtcac aaaagcgcct
    10201 taagggcagc tatggctaaa aggggataat aaaggagaga tagaatgaaa acatcattca
    10261 aacaactgtt tcgtttcgaa ttttggcaaa aatttgggaa atgtcttatg gtcgttattg
    10321 ctgtcatgcc agcggctggt ttaatgatca gtatcggaaa ctctattcca atgattaacc
    10381 atgactcagc atttttggca tctctaggca acattatcgc tcagattggt tgggctgtta
    10441 ttgttaacct tcacttgcta tttgccttgg ccattggtgg tagttgggct aaagaaagag
    10501 ctggaggggc ctttgcctca ggtcttgctt ttgtcttgat caatcggata acaggcgctt
    10561 tttatggcgt gtctagtgct atgctagctg atccagaagc aaaaatcaca agcctccttg
    10621 gcacccaaat gatcgttaaa gattatttca ccagtgtctt ggaatctcca gctttaaaca
    10681 caggggtttt tgttgggatt attgcagggt tcgtaggagc aacggcctat aacaaatact
    10741 ataattaccg taaacttcct gaagttttga ccttctttaa tgggaagcgt tttgtaccgt
    10801 ttgtcgttat tttacgttct atttttgtag cccttatttt ggttgttgtt tggccggtga
    10861 ttcagtctgg gattaacagt tttgggatgt ggattgcctc ttcacaagat tcagctccaa
    10921 tcctagcccc attcctatat ggtaccttgg aacgtctctt gttaccattt ggtcttcacc
    10981 atatgttgac gatcccaatg aactatacag ctcttggtgg aacttatgag gtcatgacag
    11041 gtgcagcagc gggaacaaaa gtatttggtc aagacccttt gtggcttgct tgggtaactg
    11101 accttgttca ccttaaagga tcagacgctt cagcctatag tcacttaatg gatagtgtga
    11161 ctccagctcg ctttaaggta ggacaaatga ttggagctac cggaacattg atgggggttg
    11221 ccttagccat gtaccgtaat gtggatgctg ataaaaaaca cacatacaaa atgatgttca
    11281 tctcagcagc ggcagcggtc tttttgacag gggtaactga gccacttgag tacctattta
    11341 tgtttgcggc gatgccactt taccttgttt atgcccttgt gcagggagct tcatttgcca
    11401 tggcagacct tgtgaatctc cgtgttcact catttggaaa cattgaatta ttgacccgta
    11461 cccctatggc ccttaaagca ggtctaggca tggatgtgat taactttgtt tgggtctcta
    11521 tcctatttgc agtcatcatg tactttattg cagatatgat gatcaagaaa atgcatctcg
    11581 caacagctgg tcgtttaggc aactacgatg ccgatatact gggtgaccat aacactcaag
    11641 caagacctac ccaagtagca gatagcaact ctcaagttgt acaaatcatt aatctccttg
    11701 gtggggcagg caatattgat gatgttgatg cttgtatgac gcgcttacga gtgacggtca
    11761 aagaccccgc taagattggc gctgaggacg attggaaaaa agctggcgct atgggcttga
    11821 tccaaaaagg caacggtgtt caagcggtct atgggcctaa agcagatatt ttgaaatcag
    11881 atattcaaga tttgctggac tcaggggctg ttattccaga agtaaatatg tcacagctca
    11941 ctagcaaacc gactcccgca aaagatttta aacacgtgac agaaaaagtg ctatcagtgg
    12001 cagacgggat ggttctccca atcacaggtg tgaaagacca ggtgtttgcg gctaagatga
    12061 tgggagatgg gtttgcggtt gaaccaacaa atggcaatat ctatgcaccc gtagctggcc
    12121 ttgtgaccag tgtctttccg accaagcatg cctttggttt actgacagat aatggtcttg
    12181 aagtgctggt gcacgtggga cttgataccg tcgccttaaa tggtgtgcct ttttcagtca
    12241 aagtctcaga aggacaacgg gttcatgcag gtgacctatt agtcgtagca gaccttgctg
    12301 ctattaaatc agcagagcgt gaaacaacca ttgttgttgc ttttaccaac acagcagaga
    12361 tccaagatgt gaccttgaca tctttaggag ctcagcctgc taagactaaa gtagctaccg
    12421 tagaacttta acaaaacatc acgagatagt gataggtaat agccatagct ggtttaggcc
    12481 ctatggctct tcttgtctta taaaggagga ttaatgacca aggtattaac actgaatacc
    12541 cattcgtgga tgcaagccaa cactcttaaa aaattagttg ctttggctga gcatatttta
    12601 gctgaaaaat acgatatcat ttgcttgcaa gaaatcaatc agttaatcga gagcgagctg
    12661 gccacagatt taccaagata tcaagctctt tcagggacac caagcattca taaagaccat
    12721 tttgctcttc ttttgatcca ttatttacaa aaacgagggc agcactatta ttggtcttgg
    12781 gcttataacc atattggcta tgacatttac caggaaggcg tggcaatctt atcaaaacaa
    12841 cctattcatg tttcagatat tctcgtgtca gcgatggctg atgaaacaaa ctaccacacc
    12901 agacgctcac tcatagccaa aaccacgtta gatggcaaag aagtcgctgt tgtcaatgtg
    12961 cacttgtcat ggtttgacaa agggttttta ggggagtggg agaaattaga aaaagagctg
    13021 ctcacactaa actgtccctt gctacttatg ggagatttta acaatccaac ggatcaagac
    13081 ggttatcaag tgatgatggg tagcccccta gacctacaag atagccataa ggttgctgac
    13141 catgtgtttg gtgatcatag catcgtggct gatattgatg gctggcaagg taacaaagaa
    13201 gccctcaagg tggaccatgt ctttaccagc aaggacttca tcatccgatc ttctaagatt
    13261 acctttgaag gcggagatgc gccagtcgtc agtgaccact atggtctaga agtgacacta
    13321 gactggcgtt agagcatgtg ctaacaagca caccacataa gagaagccaa gacacataag
    13381 aaactgcgat cgtctagggg gattaggaga acacaaagag gtctaaccag ctcaaggtag
    13441 agctggttag acctctttag tcatgtcagt aaccctagaa aaccagggct ttttttgtca
    13501 ccaaccatct gagcttcaca gaaagtacca ccaagcgtat agttgccttg ctaaagtctg
    13561 catgggggta tttctaggag tgcttggtgt gatcccaagc cccacgaagc accatggacc
    13621 acctcttcaa aggctgagaa atcataaggc gtggggtaag agacctctgt agttattgtc
    13681 ctaaaagcgc tcttttttaa aacagctatg atcatctata gacggtctag ctgttaggca
    13741 gtgtctgtgt gtcgttagac gagtgtctgg agctggcctc catgatgacc tgggctaacc
    13801 gcgaaatgtc atcaggagtc cctcgcagct caaaatcacc tagcagtgga aagccaaagc
    13861 gtttggcata ctgtttggct gtcaggcaat actgatggtt aaagtttttg ttgcctgaac
    13921 caataatccc aagacaacgc tgggcgttgc catgggcagc aataaactct cccaaaggag
    13981 tggtgaggat ctccatctca ccagagtcaa cgccatttcc gccttctaga taagtgggta
    14041 gaatcgccac aaactcttct ttgacgggaa aagtctcgtg cttcaaatct ttaatgttaa
    14101 tctgtttgac atggtaatca taattgtctg ctaaataaag ggatagccgc ttgacaaagc
    14161 taagggtgtt gccactgagg ctaataaaaa caagggtaat ctgaggcata agtgtctcct
    14221 ttttttatcc tacaaggttt cttcataagc ttaccacaat caagaccaac aaacaagtaa
    14281 agaagaaacc tagcggtatc caaccagaca agaaggtaca agcagtcaaa aaaagtaaca
    14341 tttcaaaaaa gagtgacctt aaagaaagga tccgtatgcc ctcgacttaa aaacgaggca
    14401 ggtaagtgcc aaagtggccc tctgatctcc acaaaagagc gatcagtcaa aatctaaaag
    14461 atgaacaaca aaagagaact ttacaatcgt tttcacactc tattataata ctattataaa
    14521 taatcaggtc tagctaccat gttattttat ctaaaacaca ccatctaata aagaaagaga
    14581 gaacaacata tgttgacatc aaagcaccat aatctcaaca aactggtctg gcgctacggg
    14641 ctaacctcag ctgcagccgt ccttctagcc tttggaggcg gggcaagcag cgttaaggct
    14701 gaaggtatga cgtctggaga aagaacacaa aaaatcaatg agattaaaga atttataaaa
    14761 aaaatcccaa aaacagatca ggagcaattt cggcaccgac catggtatgg tggcgactac
    14821 tttaaagacg aagactatca gaaggaactg aataaattta cggaagatgc acttaaaaaa
    14881 attgtagact tgattgaaaa aggtgcaaca aaaggtgaaa aaggggatgc tggtcctaga
    14941 ggcgagcgcg gcccacaagg tccagtaggc ccagcaggta aagctggtgc taaaggtgat
    15001 aagggtgaag ccggtgcccc aggtaaagac ggcgcccaag gacctaaagg cgacaaaggt
    15061 gaaaccggtg aacgcggtga aaaaggcgaa gctggaatcc aaggcccaca aggtgaagct
    15121 ggtaaagatg gcgctccagg taaagacgga gctccaggcg agaagggtga aaaaggtgac
    15181 cgcggtgaaa ccggagctca aggtccagta ggcccacaag gtgaaaaagg tgaaacgggc
    15241 gcacaaggcc cagcaggccc acaaggtgag gcaggcaaac caggtgagca aggcccagca
    15301 ggcccacaag gtgaagcagg caaaccaggt gaaaaagctc cagaaaagag cccagaagtg
    15361 acaccaacac cagaaatgcc agagcaacca ggtgaaaaag ctccagaaaa gagcccagaa
    15421 gtgacaccaa cgccagaaat gccagagcaa ccaggtgaaa aagctccaga aaagagcaaa
    15481 gaggtaactc cagctccaga aaaacctgct gacaaagaag ctaaccaaac gccagaacgc
    15541 cgcaatggca atatggctaa gacacctgta gccaacaacc acagacgtct accagcaact
    15601 ggtgagcaag ccaacccatt ctttacagca gcagcagtag cagtgatgac aacagctggt
    15661 gtcctagccg ttacaaaacg caaagaaaac aactaatcct ctgaattgag agaccttact
    15721 gataagcaaa cataatggag agagcccctt ggttctctct atttttggtc gggagcaggc
    15781 ctttggtatc atgctcacgc ctgagagatc ccaaaagata tcaaaagcag ctcactaacc
    15841 gttttctaag aaaaggtcac actaagcgct ttcttagcag attctgcctt ttgcttttgt
    15901 gtgtaaagtg ttaaaatagg gaaaagaaat gctagatgag gaacgaaatg gcaaaaataa
    15961 tgaacgtaac aggagaagaa gtcattgcct tagcggccac ctatatgacc aaggctgatg
    16021 tggcttttgt ggcaaaggct ttagcatatg caacagcggc ccatttctac caagtgagaa
    16081 agtcaggcga accctatatc gtccatccga ttcaggtggc ggggattctg gctgatttgc
    16141 atctggatgc tgtgacagtt gcctgtggct ttttacatga tgtcgtagaa gatacggata
    16201 ttaccttaga tgagatcgaa gcagactttg gccaagatgc tcgtgatatc gttgatggtg
    16261 tcaccaagtt aggtaaagtt gagtacaaat ctcatgagga gcaactcgcc gaaaaccatc
    16321 gcaaaatgct gatggctatg tccaaagata ttcgcgtgat tttggtgaaa ttggctgacc
    16381 gcctgcataa tatgcgcacc ctcaaacatt tgcgcaagga caaacaagag cgtatttcgc
    16441 gcgaaaccat ggaaatctat gcccccttgg cgcatcgttt ggggattagt cgcatcaaat
    16501 gggaactaga agatttggct tttcgttacc tcaatgaaac cgaattttac aaaatttccc
    16561 atatgatgaa agaaaaacgt cgcgagcgtg aagctttggt agaggctatt gtcagtaagg
    16621 tcaaaaccta tacgacacaa caagggttgt ttggagatgt gtatggccga ccaaaacaca
    16681 tttattcgat ttatcggaaa atgcgggaca aaaagaaacg attcgatcag atttttgatc
    16741 tgattgccat tcgttgtgtc atggaaacgc agagcgatgt ctatgctatg gttggctata
    16801 ttcacgagct ttggcgtccc atgccaggcc gcttcaagga ttatattgca gctcctaaag
    16861 ctaatggcta ccagtcgatt cataccactg tgtatgggcc aaaaggacct attgagattc
    16921 aaatcagaac taaggacatg catcaagtgg ctgagtacgg ggttgctgct cactgggctt
    16981 ataaaaaagg cgtgcgtggt aaggtcaatc aagctgagca agccgttggc atgaactgga
    17041 tcaaagagct ggtagaattg caagatgcct caaatggtga tgcagtggac tttgtggatt
    17101 cggtcaaaga agacattttt tctgagcgga tttatgtctt tacaccgaca ggggctgttc
    17161 aggagttgcc caaagaatca ggtcctattg attttgctta tgcgatccat acgcaaatcg
    17221 gtgaaaaagc aacaggtgcc aaggttaatg gacgtatggt tcctctcact gccaagttaa
    17281 aaacaggaga tgtggttgaa atcatcacca atgccaattc ctttggccct agtcgagact
    17341 gggtaaaact ggtcaaaacc aataaggctc gcaacaaaat tcgtcagttc tttaaaaatc
    17401 aagataagga attgtcagtg aataaaggcc gtgatttgtt ggtgtcttat tttcaagagc
    17461 agggctacgt tgccaataaa taccttgaca aaaaacgcat tgaagcgatc cttccaaaag
    17521 tcagtgtgaa gagcgaagaa tcgctctatg cagccgttgg gtttggtgac attagtccta
    17581 tcagtgtctt taacaagttg accgaaaaag agcgccgtga agaagaaagg gccaaggcta
    17641 aagcagaagc tgaagaattg gttaagggcg gtgaggtcaa acacgaaaac aaagatgtgc
    17701 tcaaggttcg cagtgaaaat ggggtcatta tccaaggagc atcaggtctc ttgatgcgga
    17761 ttgccaagtg ttgtaatccc gtacctggtg atcctattga cggctacatt accaaagggc
    17821 gtggcattgc gattcacaga tcggactgtc ataacattaa gagtcaagat ggctaccaag
    17881 aacgcttgat tgaggtcgag tgggacttgg acaattcgag taaagattat caggctgaaa
    17941 ttgatatcta tgggctcaat cgtagtggcc tgcttaatga tgtgctccaa attttatcaa
    18001 actcaaccaa gagcatatcg acagtcaatg ctcagccgac caaggacatg aagtttgcta
    18061 atattcacgt gagctttggc attccaaatc tgacgcatct gaccactgtt gtcgaaaaaa
    18121 tcaaggcagt tccagatgtt tatagcgtga agcggaccaa tggctaagaa aggataaaac
    18181 catgaaactt gtcttgcaac gggtcaaaga ggcctctgtt tctattgatg ggaaaattgc
    18241 tggcgctatt aaccaagggt tgttgttgtt agtaggtgtg ggaccagatg ataatgcaga
    18301 agatttggct tacgctgtgc gaaaaatagt caacatgcgc attttttcag atgctgatgg
    18361 caagatgaat cagtctatcc aagacatcaa gggcagtatc ttatctgtgt cgcaatttac
    18421 cctttatgct gacaccaaaa agggaaaccg tcctgccttt actggtgctg ctaagccaga
    18481 tttggctagc cagttgtatg atagctttaa tgaacaattg gcagagtttg tgccagttga
    18541 gcgtggcgtt tttggggcag atatgcaggt tagtctcatc aatgatggac cagttacgat
    18601 tattttagac acgaaatgtc actagtctct acaactaaga ggttagaaaa aagcaagcaa
    18661 ctgttgatag cgtgttgctt gctttttttg tcagttaatc tcatcgtttt agaagatcgt
    18721 ggttatttgt cgttagggtt atcaggtata ggtgtccctg tataacgcag gtagctgtaa
    18781 acttctcgtt cttcttcggt ataacgatct ttatcataag ctaaatgata gctagcattc
    18841 tctccttcgg gtcgcttgcc catataaacg gttatgatac ggttggtgtc attgtgatta
    18901 tcctctactt ttccagttaa ggtatagtcc ataataccaa aagcatcgag attgttgtag
    18961 agtagtttag ccttatcacg aggatcgtat aaatctctga agtctaagtt acgttcgcta
    19021 gctgttaaga gctgctcgct ttttagcaat tcgttggtgt tgacatcaac gtaattgatg
    19081 gtgaacagtt tcaagtgact gcgatcaaag ggatcatacg gcttttcccc ttttttaagg
    19141 acgtaatatt tctcagagat aaggtcagtg ttgttcgttt tttctacaat acctgtttta
    19201 gaattggcct tataagcttg ttcccggtct ttgatatggt aagtaaactc ttgatccatt
    19261 ggtaaaatcg tacggaaaat gtcattgtca tgagtgacga ttgaggagtc acgttttgta
    19321 atgatataat ctggatattt ttttgatagg ataaattggg caatcgctgc taactcttgt
    19381 gatgaaagag agtcaccaac tgccagtgtg gtcaattggt attggtttct taacaacggt
    19441 gtaaagtcta aatctcctgt ttcggagaca aagctcactt catagttgac gttaacgcgt
    19501 tcagctgagt tgtgaacggc tttaggttga tacggtctaa cgcgcacatg cccgcttagc
    19561 aaaaattctt ggacaggttg ggtcggcaag gttaccgaat catctcggtc agcaaagtag
    19621 accttgccgt ttcgatcagt aatggttgca tcgcttgcaa aatcaatgac ctcaaagtag
    19681 ccgtcgttac tgtgaacgtt agcgatcaat tgttcttgaa tagcctttaa taagtcagct
    19741 ttttcaagtt tatgtggcat tgcgccttta tttgtagcaa atggttttga ttttggactt
    19801 aagccttgct ctgtctttcc tccctgagca ggtcctgatg ttaggtcaat ttcaaaaaat
    19861 ttaaggctaa tttcttgatt cgtcccctca acagtaccgg caacgctaac aactaattgg
    19921 atgttgttga cagatggacg gtctagcagc cattcaggtc cagcaatagc ttggacaggc
    19981 ttgactgttc caaatgttaa tgcaaacagc agtgcaaaca tcccaaaaga taagtaattt
    20041 ttcatagaaa cctcctaaaa gttaagtttc aatccccatt attagaataa cataataatt
    20101 ttatagatac aaaca
//
LOCUS       DAUELZ010000025        19424 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.025, whole genome shotgun sequence.
ACCESSION   DAUELZ010000025 DAUELZ010000000
VERSION     DAUELZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19424
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.025"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..920)
                     /locus_tag="VHI09_000685"
     CDS             complement(<1..920)
                     /locus_tag="VHI09_000685"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEQ6120577.1"
                     /translation="MEIEYIKDYRDINQEDDTVLILGYFDGLHRGHKALFDKAREVAN
                     KEGLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNRTDSDYLTRNFEGQVYTIEEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     VRDMEKFEGIEDLTDRLEYDKRTALNWKKD"
     gene            complement(949..1833)
                     /gene="truB"
                     /locus_tag="VHI09_000686"
     CDS             complement(949..1833)
                     /gene="truB"
                     /locus_tag="VHI09_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEQ6120578.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            complement(1949..3289)
                     /locus_tag="VHI09_000687"
     CDS             complement(1949..3289)
                     /locus_tag="VHI09_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEQ6120579.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELQKRLINEI
                     ALLEEKAKHQLHEVVAKKEIAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANEQVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFVKDNQLSSRGSKGDYIYREVDTNGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALIKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVKRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFEALKDQ"
     gene            complement(3386..4342)
                     /locus_tag="VHI09_000688"
     CDS             complement(3386..4342)
                     /locus_tag="VHI09_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ6120580.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGSQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            complement(4353..4949)
                     /locus_tag="VHI09_000689"
     CDS             complement(4353..4949)
                     /locus_tag="VHI09_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ6120581.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLINL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDLPIGQALLDAADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            complement(5041..7677)
                     /locus_tag="VHI09_000690"
     CDS             complement(5041..7677)
                     /locus_tag="VHI09_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ6120582.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWPKRETDLVLSSSLAKNYQIGDELVVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRAALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPIIGYQKVSYASLTLPVKGLPDKQSISILSSSATSLSPYFNLLDS
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNANHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNCHLKKVDMLEALKSID"
     gene            complement(7692..8393)
                     /locus_tag="VHI09_000691"
     CDS             complement(7692..8393)
                     /locus_tag="VHI09_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120583.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            8511..9053
                     /locus_tag="VHI09_000692"
     CDS             8511..9053
                     /locus_tag="VHI09_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ6120584.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            complement(9196..9837)
                     /locus_tag="VHI09_000693"
     CDS             complement(9196..9837)
                     /locus_tag="VHI09_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ6120585.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(10000..10488)
                     /locus_tag="VHI09_000694"
     CDS             complement(10000..10488)
                     /locus_tag="VHI09_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ6120586.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTSAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            complement(10499..10699)
                     /locus_tag="VHI09_000695"
     CDS             complement(10499..10699)
                     /locus_tag="VHI09_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ6120587.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            complement(10740..11279)
                     /locus_tag="VHI09_000696"
     CDS             complement(10740..11279)
                     /locus_tag="VHI09_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ6120588.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            complement(11292..11480)
                     /locus_tag="VHI09_000697"
     CDS             complement(11292..11480)
                     /locus_tag="VHI09_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEQ6120589.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            complement(11491..12102)
                     /gene="amaP"
                     /locus_tag="VHI09_000698"
     CDS             complement(11491..12102)
                     /gene="amaP"
                     /locus_tag="VHI09_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEQ6120590.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVQLRHKNK
                     KGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRKFKIIVKGRLASRVAVADQIS
                     GVKEGIEKGLTEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            complement(12139..12387)
                     /locus_tag="VHI09_000699"
     CDS             complement(12139..12387)
                     /locus_tag="VHI09_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ6120591.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            complement(12751..15069)
                     /gene="pcrA"
                     /locus_tag="VHI09_000700"
     CDS             complement(12751..15069)
                     /gene="pcrA"
                     /locus_tag="VHI09_000700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEQ6120592.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALSLNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KTQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
     regulatory      15385..15471
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            15599..16921
                     /locus_tag="VHI09_000701"
     CDS             15599..16921
                     /locus_tag="VHI09_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEQ6120593.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDASGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDTIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFVLM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     gene            17041..18276
                     /locus_tag="VHI09_000702"
     CDS             17041..18276
                     /locus_tag="VHI09_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEQ6120594.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLLLSAAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKSIFSGQQVDIDPLGAIVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKVILEIPKIVAVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMILEEEIFDNVAKKLYRYEKLILSKVPDYDHYIAKSFQLVDANGQ
                     TVNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQHTSIWRRHESWSLLFHQ
                     ITPIAKRQLHHTHYRIVKM"
     gene            complement(18646..19419)
                     /gene="cfa"
                     /locus_tag="VHI09_000703"
     CDS             complement(18646..19419)
                     /gene="cfa"
                     /locus_tag="VHI09_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEQ6120595.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTILMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPNLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKSFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQNALETALKS"
BASE COUNT         5804 a         3954 c         3318 g         6348 t
ORIGIN      
        1 ctatcttttt tccaatttaa tgctgttcgt ttatcatatt ccaatctatc agtcaaatct
       61 tcgattccct cgaatttttc catgtctcga accttatcta accaaacaat ctcaataatt
      121 tctccataga tctcttcgtc aaagtcaaaa atgttaactt ctagacgcaa ctctttcccc
      181 ccaaatgtca catttttacc taaactcgtc atagagcgat agattttatt agcaacaata
      241 acatttgtaa catagactcc atcagctggt aagtatgtgt tgtcaattgg agctagattg
      301 gctgttggga aaccaatagt acggccacgc gcatccccgt gaacgactct tccccttgtc
      361 gataagtcat agcctaacaa gtggtttgct ttaactacat taccttcttg aataagcttc
      421 cttatccatg tagcagaaat ttttcgatga tcttctttta tttcctcaat ggtataaact
      481 tgtccttcaa aatttctggt taaataatca ctatctgtac gattatgccc aaacttatag
      541 tcaaaaccga caacaatatg ctttgctttc aggtttttaa tataatgagt tataaaatga
      601 tcagatgaga ccttagagaa tttagaagta aaatcaacca aatataattt atttacaccg
      661 taatcggcaa atttttcata acgcttcttt ggataagtaa tatgaagcaa taactcagga
      721 gaaaaacgcg aaaaagccaa tttcggagac tctgtaaacg taaaaacgac tacttttaac
      781 ccttctttat ttgcaacctc tcttgctttg tcaaataagg ctttatgacc acggtgtaat
      841 ccatcaaaat atcccaatat caagactgta tcatcttctt ggttaatatc acgataatct
      901 ttgatatatt caatttccat aattatccat tctacttact caaactattt aaataagaac
      961 ttttttgggt ttgtattctt gatctctttt ttctaaaata gcaattactt tttcgccatg
     1021 aaaagcagct aacagtggct cttgactagg gagactaaga cggcgaccaa atgaaatttc
     1081 agtcaattca gtatcatcaa taaccatttt aggaagatca gcaacgccat actcgatcgg
     1141 taacaaaaaa gacatctctt gtttagaaac catatcagca atttctccca aagtataggc
     1201 agtctcgagg gttaatcctg ctgaagctga tcgctgcaag aaggacatat gactctctac
     1261 ccctagagct cgacctaaat caactgctaa ggtccgcaca taagtccctt tgctacaagc
     1321 aactttaaaa gagaatcggc aaagaccgtc ttcagtaaaa ttcaaagggc tggtgcgttc
     1381 aaacagagaa atagtcactt ctcttctagg acgttccaca ctttcaccag cacgcgcata
     1441 ctcgtataat ttacggccat taacctttac ggctgaatac ataggtgggg tctgtgtgat
     1501 tttcccaagg aaagtcgtca tcgtttgatc aacgagttct tctgttagca cagctggcaa
     1561 agaggacctc gcaactacct ctccactagc atcctcagtc gttgttgagt aaccgagagt
     1621 cacttgtcct tcataaactt taccagcttc tgtcatgtat tcgatcacac gagtagcctt
     1681 accaactgca attggtagaa caccgaccac atcaggatct aaggtccccc catgtccaat
     1741 ctttttttct tgtaacaact tccgtaattt aaaaactgcg tcatgagagg tcatgccggc
     1801 ttcttttttt aaattaatga ttccatttat catgtctatt tttctctcta attagtaatt
     1861 attactgatt ctattctacc gctttatcat cagttttgcc aaaataaacg cttaaccaat
     1921 tttagcaggg aaagactcac tttcaggctt actggtcctt gagggcttca aatttctctc
     1981 gcatggttgg gttttggcgg gtcagttttt taactgaaac atcctctaat ttattattag
     2041 ctaagcgtaa ctgattttca ctagtggtta aaaagcgttt cacttcctcc atgcgtttaa
     2101 tcgatttatc aatctcgtca atagcctttt taaagttgtt gctggcagac tgatagttct
     2161 tagcaaaggc attcttaaat tgatccaaat cctcttcgaa gtgagtgatg tcaatatttt
     2221 gttccttaat caaggccaac tcttgcttat aatgtaaact attcagggct gcatttcgca
     2281 gaatccctat caactgaata aagagttgag gacgaaccac atacatcttt tgatactcat
     2341 gactgacatc aacaatccct gtattgtagt aatcattatc agcttctaac atgcttacca
     2401 aaacagcata ctcacaatct ttttcgcgac ggtctttatc taactccttg aaaaaatcac
     2461 tatttttatg ttttgttttg gtggtgtctg cctcgttctt catctcaaac atgatagaga
     2521 ggatttctac accattggta tcaacctctc ggtaaatata gtcccctttt gacccacgac
     2581 tagacaattg gttatctttg acaaagctag cattgggaaa agcatagctt cttactttat
     2641 tgaactctgt ttcagcatag agttctagac tctctccgat tgctttggta gattgctgcg
     2701 ctttgaaatt tttataaaat tcaacttgct cgttagccgc ttttaactgt gcttcgtagt
     2761 cgctgcgcac cgaagccaac gacaattcac tttctttagc ttgaagcgcc aattggtttt
     2821 tgattccatc gcgttctttt tcgattgtag caagttttga ctggaacgtg gcatcttttt
     2881 caagtgctaa tttgtccagc tgattggtca aactaaccac ttctttgtcc ttctcagcta
     2941 attgattggc cagttccaaa gcattttgtg aggccaattt atcaagtttc gcttctaaag
     3001 aagcaataag ctggtctttt ttagccagtt cttcttgtct gaggtaggct tgttcttttt
     3061 caatttgctc taattggttt gtcaagctgg taatagctat ttcttttttt gcgacaacct
     3121 catgcaattg atgcttcgct ttttcttcta gcaaggctat ctcattgatt agccgttttt
     3181 gcaattcttc atcaaaggct tgccctctga cttgttccaa taattgacta tactctgact
     3241 cattgatagt aaatagggta tgacaatgag ggcattttat ttcgttcatc ttgtctccat
     3301 tctgagattc ttctttttat tatagcaaaa aagctcagga agttaagtcc caagctgtca
     3361 tagataagaa gtgtgcaata gagatttagt cattttgata atcctgataa aactctacct
     3421 ttaataagat aaagcgctct aagtcttgta agagctgaaa taaaaccgca cgtcgctcaa
     3481 attcctcccg agtttgagga agatcaccat gacgaaaggt ttcaagcaat tgttcgatgt
     3541 catcaatcaa ggttaaagcc ggattttgtt cgcttagctg acaagcagtt tcgtgaaaaa
     3601 gatgtgacaa aagaatactt tccttacttt gtctcattaa cgtgtttacg ttgatagcca
     3661 tttgtcccaa tagtctattt tgttgtcgtc gcatctcaaa ataatgaact tgataattgg
     3721 tctgctgaaa tagttgatta tgcctttctc gataaactag ttttagagct tcatctaata
     3781 ttttatcaag gtttttgatc aagagccctt cattttgacc ttttccttcc agtagaaaac
     3841 tttcaaaacg gtataagata cctttcagat ccgattcaac tttttggtga tagtaccgaa
     3901 tttgctgatc ttgtgaaccc atataagcat taaatagtaa agcgacacta gtccctacaa
     3961 aaaagagcat aaactcattc gataaaatag gtaatgcaat actcttttta gcaatcaaat
     4021 gtgtcacaag tactgttata ggaaccagtc ctgcctctat ctgaaagtga tagagcaaag
     4081 gaataataat taacaaataa cacataaaac ctactgtcgt aaaaccaaac aagctaaagc
     4141 acatcatcgc aatcccaaaa gccaggaaaa aagatagcaa gcgattccta gcgaccacca
     4201 aactggactt acgggtgtct aaaacactta acagggcaat gatccccgct gacattgcat
     4261 aatccagatg aagttggtaa gcaattaaaa tggcaacaat ggtagccaag gtcattttta
     4321 atgtacgttc aactgaaccc atgacaagac tcctaaccta atcctttttg aatttcattc
     4381 tgaatatatt cccactcctg ttctgctcga ttgagtttac gatgataatc tgttaatcct
     4441 ctcaagtagt gattaatcaa ataatcctgt ttcactaaag gaataacggg aattggaata
     4501 tccaacaatt ctttcgttga gagattgatc acatctttgc catggtccgc tgcgtccaag
     4561 agcgcttgac caataggtaa gtccaaaaag aacttgatat agtagcccct taacagtttt
     4621 tgagggcgta agacagtgat atttgatgat gccaccacat ctcgattttg cttatgaaaa
     4681 acacagactt tttttagggt tcccttagag gcgattaaga catctccgtc ttccaaaagg
     4741 taacgcaaga gctgcctacg atccatctgg aaagttctta attggtgata ttgaattcct
     4801 aaggtgccca tgtcagataa attaatcaat cccacatcac ctggcacaac cttacttgag
     4861 actgcttttc ctttaaaaca atcaacaact gtacctaatg ttaccttttc tctgctatct
     4921 gctagatagg cctcaatgct attttccata catagtatct ttctattaaa ttatagtatt
     4981 tagatactat cataatatga ttaacttttc aagtgctgtc tatctctgtc ttttcaccaa
     5041 tcaatctatg gatttcaaag cttctaacat atcaaccttt tttaaatggc aattaaccca
     5101 gatacctaat accgctaaca agctaaggat tactagaatt ggcactagat aaacataagc
     5161 atcaaccttt acaccaaatt gaatatcccc agttgaaatc attgtcatga tataagtatg
     5221 caggcctttt cctaaataga tacctaaaag aatgcctact agggatagcg aaatagtttc
     5281 tcgataaata tataaggtga cctcctgatc gtaaaatccc agaactttaa tagtggataa
     5341 ttctcgaata cgctcagcaa tattaatagt cgttaagtta taaaggataa cgattgctaa
     5401 taaaatagac aagaagacaa ggagggtcat gacctgatta agagaggtga ctactagctg
     5461 aacagattta atatgattgg cgttttgtga aactgctcta atcgctttta aggttaacaa
     5521 gtcactggct gtctccttta tgtgcttgct atctttgtct tttaccttaa tcagataggc
     5581 aggagcagcc tccaatcctt taaaatgatt cttaaaatag gtattagaca ttatcaggta
     5641 atggccaact gtcatgtcaa taacctgttt aatcgtcact ttataagact gtcctttccg
     5701 atcagtcaat accaactgat cacctggttt taccttgtaa taggaggcta atttctcaga
     5761 aatcaaaaca ccagaggttg gaatgggaac cttcttttgc tcctgactat ccagcagatt
     5821 aaaatagggg ctaagagaag tagctgaact tgataaaatg gaaatacttt gcttatcagg
     5881 taatcccttt acaggaaggg ttaagctggc ataagatacc ttttggtacc ctataatagg
     5941 ttcttgttta agatagctag ctaagtcagt ttgctctgtc gctgtcgctt tggccgaacc
     6001 gacagccaaa atatcataag tcgttaaatc accaaattga tgctcaacta ctttggctaa
     6061 tgacgactga atccctaagc ctgcaaataa aagggctact gagcctgcaa cgcctacaat
     6121 ggtcatcagc attctttgtt tatagcgaaa aatattacgt atggtcacct tgtgagtaaa
     6181 ggacagagct ttccagacaa aagtaaggtg ttccaaccag atttttgccc ccttactagg
     6241 aggtttgggc agcaataact gagctgcatt gaggaataat tcctttttga caattaagta
     6301 ggctggtaaa acagcagata acatagccag caagtaagct atgccattat aaaaccaata
     6361 actataaagg tgagtctttc caatagtcaa agctcctgtt aagatctctg aaatcaaggc
     6421 agataagagg taagttcccc caataatacc taaagttgtc cctaaaaaac tggctaaaag
     6481 cccatagata agaaacttta aactgatatc cttgttagaa taaccgatgg cttttaatag
     6541 accagagctt gttctttctt catcaacata acgtgtcatg gtggtaaagg ctactagagc
     6601 agctacgaga taaagaacaa caggaaaaat atttccaaca tttgaaatgg aggtcgttga
     6661 agttgcataa gtatgatatc cttctcctcc tggtagggtc gagcgattat aagtcaggta
     6721 actgggtttt tctagcttat cttttgtagc ctgcacctgc tctaactgct tttcctcctt
     6781 ggctaatgct tgtttgcctt gttcaatctg atgcttagct tcttgtaaag cagagccttc
     6841 taaaaaagtt aagttctcct cactcgcagc tagtttcact gtttcctttg caagtgctgc
     6901 tctgccattt tttaaggcaa ggtcatactg attttggagg tcatcataac gcttctggcc
     6961 attatcttta agtagattat ccaaatgagc ttggttttgg gttacccttt tttgatagtc
     7021 tttggaaaaa gcgtttgtca gacgaagatt actaaaacgg atgcgcagca ggttaaaggc
     7081 acttttaaaa acgtttggat taacaaaagc ataagcgtag agacttccat ctccggttga
     7141 ggaacttcct aaattggact tggaccaaac ttctgaagaa ttcgcaaaac caaccacttg
     7201 aaaatgggtt gtcgtcagca aaccttccat aggtgaggtg actactagtt catctccaat
     7261 ctgatagttt ttagcaagtg aggaggataa aaccaaatct gtctctcgct ttggccaact
     7321 ccctttaacc aagactggct tagacacttt ctttggcacg ctatagagcc taagagattt
     7381 ctggttgcta gttagactga catcaagtaa atgaccatat tctaaatgag ccccttttaa
     7441 cgtatctaac tcttgtttat cggcttggga aaattgatga gaagccagta ccgttaggtc
     7501 catcacctgg tgtctttcga ggtaacggct ggctgttcgt tccatatctg gaccggtgac
     7561 tttaagtcca actaaagcaa aggagcctaa ggccattagg aaaaataagg aaatgaatcg
     7621 ccctttacta ttcttgatag ctctcaaaat atccttccat aaggttttct ttatcatacg
     7681 acaccttcct cttaataatc aattgtctca atgcttgcag gttccttatt aatgactgtc
     7741 ttggtaacct gtgcatcatg cataaaaatg acacgatcag ctatcggagc aatagcagca
     7801 ttgtgggtta caataactac cgtggtcccc ttagttcgtg ccatatcctg taagagggtt
     7861 agaatttgct ttcctgtttg gtagtcaagg gcacctgtag gttcatcaca aagaagcaat
     7921 ttagggtttt tagctaaggc acgtgctatc gaaacccgtt gctgttcacc acctgagagc
     7981 tgagcaggaa aatgatccag acgatgactg agtcctactt cctttaaaat ggtcacagga
     8041 tctaaagcat ctgctacaat ttcaactgct aattcaacat tttctttagc tgtcaaatta
     8101 ggaaccaaat tataaaattg aaaaacgaaa ccaatagcat tccgacgata ctgagttaag
     8161 gccttagacg tgtaatgagc aatgtctttg ccatcaataa ttacttgacc tgcatctact
     8221 gtgtccatgc cccccaaaat attgaggact gtcgatttac cagctccgga tgctcccaaa
     8281 ataaccacta gttctccttt attaatctca aaagacactt cgtggttggc aaaaacagtc
     8341 gtctctccta cctgataaga ttttgaaacc tgttttaatt caataaaagc catctgcccc
     8401 tccattattt gaacatagtg ttgatttaat tataacctta ttatataatg gccctaagta
     8461 tttaacaaga acaaatctga cgcaactgtt gatttaagga gcacctgaga atgaccaaaa
     8521 gacatactga aaccaaagct tatgtaaaga ccgccttaac caccttacta actgaacaat
     8581 cttttgaaac tcttactgtc tctgacctta ctaaaaaggc tggtatcaat agaggaacct
     8641 tctacctcca ctacactgat aaatttgaca tgatgaatca cttcaaaaat gacactctgg
     8701 atgatttgta ccgtttgtta aaccaggctg aaatctacac agacactcga caagtcctca
     8761 atcaaacctt gtcctacctt atagaacata gagaatttat aaccgcactc gctactattt
     8821 cttacctcaa atttccgcaa ctcatcaagg atttctgtta ccaatttttg actactatta
     8881 ctggtttcca agacattgtg accaatcaat accatattcc ttatccctat gctttagaag
     8941 tctaccttgc cagtattgag agcattatca gctattggat tgctaatggc tatcaagaaa
     9001 ccactgaaga ggttgccgat attattctca aagcagttac tctagacaat taaaaaacat
     9061 caaaagagtc tgaaatcatg ctcccaatcc aaaacaactg tctaaacaga tattttggaa
     9121 agacaaacca tgatgatctg cctcttttaa taatgctaaa cagcaacaaa aaaagtttga
     9181 ggtgggccct caaacttata atccttttcg taaattaaaa aaggtcttca ttcgtgaaat
     9241 gactgactca tcaccaacga aataaatatg atcgcctttt tcaataacag aataaggacc
     9301 tggggaaacg ataaactgac cagcatgttc aatagcgacg atagttgctc ctgtctggtg
     9361 ccagatattt aacacaccaa tagatttgcc aaaatgttcc gaatcttgac tacaaataat
     9421 ctcataaggt gctaatgggt attgcttact aacagattga ctttgcatta aaaaatcatt
     9481 gaccaaaaca gccatttttt ccattgcttt tccctgatca ttaatagtct cacgtatttt
     9541 ttcttttaaa acagcgatgg aatgggttga ttcgtattga tttataaact caatagctct
     9601 ttcttttgac agcacaatag ctccactacc atgttttaaa gtcaaaattt tcaagtcagc
     9661 taaaatattc aggcctttac gagccgtttc gggtgataca ttaaatgtcg aggcgatagt
     9721 agttcttgac tttaactttt cgcccacttc atattcgcca tttgcgatac gctgagctac
     9781 tgaaatagca attttttgat attttgaact tgttatctct tttttaggac ttatcactta
     9841 atttactcct ttctaacaat ttgaaaaatt tctatctaat taaatcatat ttttagagag
     9901 atgacaatag tccgtatctt tttaaccgtg acaaaaagag ctggtaaata ccagctcaaa
     9961 cttatcacca ctagtcaata atacttccta gacgattgct tatttagctg cctcatcact
    10021 atttacagta tcagagatag tatcttttaa tttttctgtt tgttctgata caaattgtga
    10081 agtacttgaa gctgcactag tgacacgatc ttgaacagta acgctagcag cttcatgttc
    10141 ttccttagta cgaatatcaa caacgttcac attgacttca acaactttca aatgtgtcat
    10201 actatcaacg ttttgagaaa caatagcttt aatagattct gcaatagcag gaatatcttt
    10261 accgtattct acaatgattg ctaaatcaac tgctacttcc ttgctgccaa cttcaactga
    10321 aacaccatca gttacagact cagaattcac caaattattt ttgatatttg agaaaaatcc
    10381 acctgttaca gctagtaatc cgccaacgtt ttccaaagca tgaccgacaa ttttttcgat
    10441 gaccttatcg tcataagtaa gagtgctttt aatttgagtt ttttccatca tgtccccttt
    10501 atttatcttt attatctaag cctttagcta atccttttac agtatctttg gcgtctgcaa
    10561 taacctcttt aacttttcca gtaactttat ctactttacc ttcagtttct aaagatttat
    10621 ctccagaaat cttaccaaat ccttctttta acttgccacc agcttgatct aatttcgcat
    10681 tatatttttc atctgacaca ataaaccctc ctttagctta cctaattgac gacatatgat
    10741 tattttacac gtggctcagc tttttgatct tgaagctttt caacaccgtt atccactgaa
    10801 gctttaacat tctcaacttg atgactcgta aattcagatg ttgaacgtgc catgtctgaa
    10861 actttgtctt gtaaacttac tttttcagct tcaaattgtt cttttgtttt aatgtcgact
    10921 actttaacat taacttcaat cacatccaga tcggtcattc tcttcacttc ttcttcaacg
    10981 atcgatttaa tagagtcata aatagtcggc acatgctttt gatattcagc gacaatatca
    11041 aggtcaacgg caacttgttt ttttccaact tccacgttca cgccatcacg tacagattcg
    11101 gtgtttacta atttatcttt taggttagca aaaaaaccac catttacacc cagtaaccca
    11161 tcaacatttt caagtgctaa accaacaatt ttttcaatta ctttatcatc atatgttaat
    11221 tgaccacgaa ttgctgaagt taagtcttta gatgtgtttt taatataagt ttcagtcata
    11281 ataaatctcc tttatttgcg attaagaaat tgatcaataa tacctgtttt tttggcatac
    11341 aggccaccat atattcctaa aataatgaaa ataattgcta gtagcgtttt gaataaacca
    11401 aaagccatga gcaatatggc aataattagc cccaccaagc caccaataat agggtacttg
    11461 aatttttcat aaaattccat gatagatctc ctattctaca cgatttctgg taatatgttt
    11521 acgatcagaa tcagcaatat ctttaacata aactttgaaa ttaacaggat aattaattcc
    11581 gaagaactca gttaaacctt tttcgatccc ttctttcacg ccactaattt gatctgccac
    11641 tgctacacga gaagctaaac gtcctttaac gataatctta aacttccgct tatataattt
    11701 agctgtgact gttgggtttg gcatcaaacc tgcagtttga atagcggtcg caacataact
    11761 ttcaatagcc gattttttta ataaaagtgt tcctttttta tttttatggc gtaactggac
    11821 ctcagtataa attcttgggt ataaaataat aactaataag gcaagaaatg ttataacaaa
    11881 taatacaagg gctgtccaga agtaataata ggataaagct gaatctaaga gattgggaaa
    11941 actatccata ccccaactta gccaacgata actataaggt aaatatatat agcctccggt
    12001 tattccaact acccatccaa aaacagacaa tagaacaagt ccgacaagac aataactaat
    12061 ctttaacaat tttgacatac agacctcttt tctacttaac attctctatt tgtaagtact
    12121 tactaaataa agaggaaact agttgttagt tttgtttagt acaaaagaag tgatcataac
    12181 aacgataacg gcaccaataa ctgacgggat caaagacatt cctgctaaag aagggcccca
    12241 tgaaccaagt aaagcttgac caacggaaga accaactaga ccagcagcga tgtttgcaat
    12301 ccagcccatt gaaccacctt ttttagtaag tgctccagca atcacaccaa taagcgcacc
    12361 gacgattaaa gtccaaataa gtcccatgat aattctcctt tttagatact cctgtacctt
    12421 ttgtccaagg aagaataaca gctgttattc ttcaagcaat agtcttttaa tagtcgactt
    12481 tcaaaaagtg actatataaa ctaaataata aagtgttagt ttattttttg tttgaatacc
    12541 gatatttcaa aatcctataa aatgttattt tgataatatt ttataaagtg accactcgtt
    12601 atttaattta tattgttatt atacaccttt aaatttaaaa tgcaactgat atgctcctaa
    12661 aacttaaaat tttttaactt ttatatgata cagaaatcta aaaaaaatga gcttcctact
    12721 cacttatata gtaggaagct catttttttc ttattttttc tcaataggag caacacttgc
    12781 tagtaatttc tttaaaccaa cttctggaaa tttgattttc aattccatcg tttttccgga
    12841 gcctgaaacc tcaagcacag tcccgtctcc ccatttttta tggtgagcga tatcgccaat
    12901 ctgccaagtg acctcttcta cagagctatt agaaccaaac ggtaaaactg agctagttga
    12961 agctgatggt atcgtatgtg cctgcattgg ctgtgctgat tgtcttactt gagcctgggt
    13021 ctttcgagct tgtaaggctt ctgataggct catcccttgt ccaaactgtg tgtgtgtttc
    13081 agtagaaaaa cgcacaccaa atgaagattg tgctggtctt gccaatcctt tataagagag
    13141 tagttcctct gaaatttctt tcaagaagcg tgttggacgg ttataactac ttttaccaaa
    13201 gagggttctg gtgttggcat tggtcataaa gagaacttct tcagctcttg tgattccgac
    13261 ataagctaaa cgtctttctt cttccaattc atctggatct tcagaggcac gtgataaggg
    13321 gaagacacct tcttccatac caattaagaa aacaacaggg aactccaagc ctttggcagc
    13381 atgtagggtc attaaggtta cttcagcagc ttctgcttgg gaatcatctg tatcagcaat
    13441 cagtgctaaa tcgtttaaaa aacgtcccaa gcgatcaaca ccggtttcgt cttcttcttg
    13501 agaggcactt gactcgtcaa aatttttggt aacagataag aattcttcga tattttcgat
    13561 acgggcttgg ctttccaatg tattttgcag gcgtaagcta tctaaatagc ctgttttttc
    13621 taacaaagct tctgctaagt cggtgatact catctgatct aggtcttgac gtagctgacc
    13681 gagaatattg gccaagtcca tgatagcttg tgctgcttta cctttcaagg gggacatcaa
    13741 tagatttgaa gctgcttcta ataaagactg gtcactctca taggcaaact gacgcagttt
    13801 atcaagcgtt cctggaccaa cacctcgttt aggctcattc acaatccgct caaaagaaat
    13861 attatcagca gggttagcaa caatggttaa ataggcaatc aaatcccgaa tttctttacg
    13921 gctgtaaaac ttggtgcctc caaccatggt gtaaggaata ttggatttta agagtgcttc
    13981 ttcaatggtc cgagattggg cattggtacg gtataaaaca gcgaaatctt tgaagttttt
    14041 tccaagctct tgactcatat tgctaatggt tgaggccaca aatactgcct catctcgctc
    14101 atcatttgcc ctgtaataaa ccagttgttc cccatccgca ttttgtgtcc aaagtttctt
    14161 aggacggcga ttacggttat tgttaatcac gtcgttagct gcttgcagaa ttttcttggt
    14221 agagcgatag ttttcttcta aaagcactac cttagcatct gggtaatctt tttcaaaatc
    14281 aagaatattt tgcatgtcag ctccccgcca accatagata gactgatcag cgtctccaac
    14341 aacgcaaata tttttaaagc gagaagctag caatttaatt agctgatatt gagcatggtt
    14401 ggtatcttgg tactcatcca catgaatgta ttggtaacgc tgctggtaat aagctaacac
    14461 gtcaggatta gcgtcaaaca ggcgtagcgt catcatgatt aaatcatcaa agtcaagtgc
    14521 ttcgctacgt cgtaattctt cctgataagc tttgtaacac ctcgctacga tttgactata
    14581 catatcagca gcctgtgcct cgtatccctt ttcatcaagc aaatcatttt tagcgtttga
    14641 aatagttcct aaaatagaac gttcattcca ctttttaggg tctatattta attgttttaa
    14701 aatgcgcttc atcaacgttc gttgctcacc tggatccacg attgtgaagt ttcggttgta
    14761 accaatgtga tcagcctcac gacgcagaat ccgtacacac atggagtgaa aggtagcaat
    14821 taaggtatct ttagtagctg gatttaagga caaggctctt tctttcattt cacgcgcagc
    14881 cttattagta aaggtaatag ctaaaatatt ccaaggattg acaaattttt catcaattaa
    14941 ataggcaata cgatgggtca gtacccttgt tttaccagaa ccagcccccg ccataatcaa
    15001 taaggggcct tcagttgttt gaacagcttg tgcctgccga tcattcattc catttaataa
    15061 aggattcatg ctttctcctt aatctcgttc agtgacatct cttttatcaa ccttaaaatt
    15121 atatcatgtt tccaaggaaa atagcaaaga agaccccaaa agtaaagaga ttcctttttt
    15181 ctagtttagg ttcagatatt taaaaataat agcgatttct taaagcattc cttatttttt
    15241 tgctattttg gttgacttca ttcgtttttt aaggtatgat agttctaatt taataaaccg
    15301 aatgatgtca tgcaggagaa gaatttattt tcgccgaagg agttatactc tcaggtgttc
    15361 agtttttgaa cgggactgtt tgatggacgg acttctggag agaccttatt aggcgccgaa
    15421 ggggcaaggc atactgctca atctctcagg caaaaggaca gaaggtaaaa tacaaacacc
    15481 attaagaaca gtcttagtct tttttgtgtt tgctgtttta tcattgcttc agaagttgtc
    15541 tcaaagaaag agatagcttt tttcttttgg cgtcttcgat gacttttagg agagaaagat
    15601 gatagcactc gttaaattaa ttgataacct tgtttgggga ccgcccctct taattttgtt
    15661 ggttgggacg gggatttacc ttaccagtcg tttaggatta attcaaatct taaaactgcc
    15721 aagagccttt aaactcattt tttcagatga cgaaggacat ggagatattt catcctttgc
    15781 tgctcttgca actgcccttg ccgctactgt cggaactggt aacattgttg gagttgccac
    15841 tgctatcaag tctggtggtc ctggagcgct cttttggatg tgggttgccg ctttttttgg
    15901 aatggccact aagtacgctg aaggggtact agcgattaaa taccgtacaa aggatgccag
    15961 cggtcatatt tctggtggtc ctatgtacta tattgttaat ggcatgggga caaaatggaa
    16021 acccttagcc attctttttg cgggttcagg gatcctagtt gccctatttg gaatcggaac
    16081 ctttgctcaa gtgaactcga tcacctcgtc tttaggtcat agctttggct tatccccaca
    16141 aatggtcagt attgttttag ctatctttgt ggctgccatt atttttggtg gcattcattc
    16201 catttccaaa gtggctgaaa aagtcgttcc ttttatggct attttttata ttctatctag
    16261 tttagcagtt atttttagtc actaccagca gctacttcct gttattcgtt tagtctttca
    16321 atcagccttc actccgacag ctgctattgg aggctttgct ggaagtctca tgaaggatac
    16381 cattcaaaaa ggaattgcac gcggtgtctt ctccaatgag tctggactag gatctgcacc
    16441 tattgctgca gcagcggcta aaaccaatga acccgtggag cagggcctga tctccatgac
    16501 aggaactttt attgatacga ttattatctg taccctaacc ggcctatcta ttttagtaac
    16561 aggccaatgg actggacagc tcgaaggggc tcctctgacc caatctgcct ttgccactgt
    16621 gtttggaaat ttagggactt ttggcctaac cttttcccta gttctcttcg cctttacaac
    16681 gattttagga tggagttatt atggagaacg ctgctttgaa ttcctctttg gcattacaca
    16741 tctcacctac ttccgtattg tctttgtctt aatggtaggt ctaggaggct tccttaagtt
    16801 agaactgatt tgggttttgg ctgatattgt caatggttta atggcacttc ctaacctgat
    16861 tgccctccta gctctctcgc cagttgttat tttagaaacc aagcattact ttattaagta
    16921 aacattacgt tagtactacc cagttatacc tagtcttctt ctgccaagtt caagaagcta
    16981 ggcttttttc ttaagcccct ttttatggta taatataaag ttgaaatcaa aggagttatt
    17041 atgacacagg atccgattgc aaatttaaaa ctagccagaa agggcccaat cgttagtatt
    17101 attgtctact tgttgcttag tgctgccaaa ttattagctg gttatttgtt aaatgcaagt
    17161 tcccttattg ctgatggatt taacaattta tcggatattg tgggaaatgt agccctgctt
    17221 attggtcttc acttagctag ccaaccagcc gatgccaatc ataaatttgg tcattggaaa
    17281 attgaagatt tatccagcct tgtcacttct tttattatgt ttcttgtagg tttccaagta
    17341 ctgattcaca caattaaaag tatctttagt ggtcagcaag ttgatattga ccctcttggg
    17401 gctattgtcg gtatcgtttc agcttttgtt atgttagggg tctatgtctt taacaaacgt
    17461 ctttccaaac gtgtaaaatc cagtgcctta gtcgctgctt ctaaggataa tctagctgat
    17521 gctgttactt ctatcggaac atcaattgct attatagcag cttctttgca tttaccagtt
    17581 atcgatcata tagctgctat gatcattacg ttctttattc ttaaaacagc ttttgatatc
    17641 tttatggaaa gttcgtttag tttatctgat ggatttgata gccgtcattt gaaaaaatac
    17701 gaaaaagtca ttttagaaat ccctaaaatt gtcgccgtta agtctcaacg agctaggacc
    17761 tatggtagca atgtctatct tgatattgta cttgaaatga atcctgatct ttcagtctat
    17821 gaaagtcact ctattacaga gaaagtggag cagttattga gtgaccaatt ttctatttat
    17881 gacattgata ttcacgttga acctgccatg attctcgaag aagagatttt tgataatgtc
    17941 gccaaaaagc tctaccgcta cgaaaaatta attttgagta aggttcctga ctatgaccac
    18001 tacattgcta agtctttcca actggttgat gcgaatggcc aaacagttaa ctatgaacaa
    18061 tttttgaacc aagaaattta ttatccaagt aacttcaacc attttcagat tgaatccatt
    18121 agtcaaaaaa cgatgttggt aacttaccaa ttaaatggca atcaacatac cagtatttgg
    18181 aggcgtcatg aatcttggtc cttactcttc caccaaatta cccctatcgc taagagacaa
    18241 ttacatcaca cacactatcg tattgtaaaa atgtaaaaat attacttatc ttataacata
    18301 actatacttc atgatattcc taaagcgatt cctagtccca aaataataag ttaaatagtt
    18361 aaaaaaatcc ctacaatttg tttttaaaca gatcataggg atttttttaa gaatacacgt
    18421 tttcatattg aatcttatta gtttaggagg ttaaacaaga ttcaatagtg acggttgaac
    18481 aataccttaa attggtacat catgttaaag gcaaaaaggg ctacaggtga ctcattgttt
    18541 atgagagcat cacctcaaac tctacaatgg taagatattt aaaatcttta cctattctat
    18601 atcctaatat aatcatctac ctatacaaca cattacttat acactctatg acttaagagc
    18661 tgtttccaaa gcattttgca cagcaataac ttcatcgtca acttgttgaa cagtggttgt
    18721 cggatttagt tggacaccaa ccgcatgtgt gatagctttg ttaagtctat tataaacgtc
    18781 aaatgatttg atccctaaaa cctttttatc tctttctaaa cgagtgttcc aaatagcttt
    18841 atttagttta gctttagtat agatagtagc acggtctgtt ggctgtaaat taggataatt
    18901 gataactttt tcctcaagtg ctttaatttc ttgtacttga gctttgatag cgtcaacaga
    18961 tgcaaatgga tctataatac gaatgactaa tttagtaata gcaaacccca tatcagtatg
    19021 cgctttttct actttgtgag ttaattgggt tgtggaaaat acgattgctt tgatagcatc
    19081 tgataatgct tctactcttg ccccaattga actaaaatca tagatcgttt cagtattgaa
    19141 gcgtatagaa ctcttcatgt gatcaacagc atcgatagct ttgttaacag tagcctcaat
    19201 atcagtacct ttagcttcat gtttaatagc ttctaattgt tgatgtccct catcaagctt
    19261 ttgcactaga gcatctcttt gaagattcgc ttggttgtta ttcattaaaa tagtatcggg
    19321 atttgaagca tcatcagcta aaactgagtg cgtcatagtt gaaaataaag ctaatccagc
    19381 aacaactgtt gaaagacaga gaaggtgttt gggtttcata ttat
//
LOCUS       DAUELZ010000026        19290 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.026, whole genome shotgun sequence.
ACCESSION   DAUELZ010000026 DAUELZ010000000
VERSION     DAUELZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19290
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.026"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            235..1083
                     /locus_tag="VHI09_000704"
     CDS             235..1083
                     /locus_tag="VHI09_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEQ6120596.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLFETSQFIYLNHQQVLPADLVELLQ
                     QMIAGLEVILPISTTQTAFLCRQATSIKVLRSLEGLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQFLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
     gene            1136..1279
                     /locus_tag="VHI09_000705"
     CDS             1136..1279
                     /locus_tag="VHI09_000705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120597.1"
                     /translation="MIDRSACFGSCDWPVFTWFSWFMVPYDLTSPAIVQVAQRSLVAL
                     SLF"
     gene            1381..2514
                     /gene="ugpC"
                     /locus_tag="VHI09_000706"
     CDS             1381..2514
                     /gene="ugpC"
                     /locus_tag="VHI09_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEQ6120598.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLAIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            2644..4257
                     /locus_tag="VHI09_000707"
     CDS             2644..4257
                     /locus_tag="VHI09_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEQ6120599.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTSGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDLEITGNGPRLHDYL
                     KEMNQVTFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAKELDVPALKTIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSEAKETWLPVNPNYQDINVADALADPDSIFYTYQK
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFATDLAGAQ
                     VIIANTDVDTVLKTKHLQPWDAFCLKLKA"
     gene            4455..7952
                     /locus_tag="VHI09_000708"
     CDS             4455..7952
                     /locus_tag="VHI09_000708"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEQ6120600.1"
                     /translation="MKKKVNQGSKRYQHLLKKWGIGFVIAATGTVMLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGFQSSSKEAKPVLDSSSANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWTDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKNPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDKDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTKVVGQADL
                     TKSDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSVNPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSIAYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYRTKVS
                     DDKVPNKATLIDAVAKYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDVIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDSLTALVLKV
                     SSKPADPSQKKSQTGNHQTKTPDGSKDLDKSLMTRPKRAQTNQKLPKTGEASSKGLLA
                     AGVALLLLAISLLMKRPKD"
     gene            8094..8441
                     /locus_tag="VHI09_000709"
     CDS             8094..8441
                     /locus_tag="VHI09_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEQ6120601.1"
                     /translation="MTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRSSCPYCRRFA
                     PKLAQVATDNQKDVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQHQRAVCDSS
                     LTPDDILAFLTRE"
     gene            8557..8916
                     /gene="yajC"
                     /locus_tag="VHI09_000710"
     CDS             8557..8916
                     /gene="yajC"
                     /locus_tag="VHI09_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEQ6120602.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDSAIESH"
     gene            9135..9884
                     /locus_tag="VHI09_000711"
     CDS             9135..9884
                     /locus_tag="VHI09_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEQ6120603.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNRRQRRFGKV"
     gene            9897..10691
                     /locus_tag="VHI09_000712"
     CDS             9897..10691
                     /locus_tag="VHI09_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEQ6120604.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMMIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            10764..12023
                     /gene="rseP"
                     /locus_tag="VHI09_000713"
     CDS             10764..12023
                     /gene="rseP"
                     /locus_tag="VHI09_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEQ6120605.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLEETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMPDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNTLKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            12221..14077
                     /locus_tag="VHI09_000714"
     CDS             12221..14077
                     /locus_tag="VHI09_000714"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEQ6120606.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPART
                     DLDRWVVLDKSIASMDDIPKEVLEEIKAELAAWMISGEDTIAYSTESSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRTVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH"
     gene            14331..18728
                     /locus_tag="VHI09_000715"
     CDS             14331..18728
                     /locus_tag="VHI09_000715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEQ6120607.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRNNHLPNLVKQFEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVKKAVQDNLEAQKSLEAMMPPVEEATPAPKFDYKER
                     AAKRQAGFEKATITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHERKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQGAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLKDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLAKYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRANKLVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVENTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGAEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     VLGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMGILGNMPEDNQLSLFDDFF"
     gene            18838..19266
                     /locus_tag="VHI09_000716"
     CDS             18838..19266
                     /locus_tag="VHI09_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ6120608.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
BASE COUNT         5657 a         3700 c         4411 g         5522 t
ORIGIN      
        1 gataatgtca tgataatgaa aaaagagaaa caatacctgt tttagtataa acataaatta
       61 atcttccgat aaatagctat ttgattgaat gttattgcta aaaatgaata accattattt
      121 agttttaatg attaaaaagt caaaaaatac aaaatcgtgc tgacaacaaa ccgaactgtg
      181 tgataaagag tcatgtgtta taatggttta aagaccgtca tgaaaggaat cgctatggag
      241 ctaaaagact attttccaga gatgcaagta ggccctcatc cgttaggaga taaagagtgg
      301 gtgagtgtca aagaagggga ccaatatgtg cattttccca aatcatgttt atccgaaaaa
      361 gagcgcttat tacttgaggt ggggctgggt cagtgtgagg tcttgcagcc gctagggtca
      421 ccttggcagc ggtatttgct tgatcatcag ggcaatcccc ctcagctgtt tgagacttct
      481 cagtttattt acctcaatca tcagcaggtt ttaccagctg acttagttga gttgctccag
      541 cagatgattg cagggcttga ggtcattttg cctatcagca caacccaaac agcattttta
      601 tgtcgacaag caacttcaat aaaggtcttg cgctcgcttg aaggtctctt gccaacgcta
      661 gaaagtgatt ttggtttggc cttaaccatg tttgttggaa atgcctggta tcaagtagct
      721 gcgggtacat tgagagagtg ttttgaggag gaatgtcagc tgttaacggc ctatttaagg
      781 caacaatcag gcagaaaatt attaacattt tcaggactga tgctgtggtc tttgctgtcg
      841 caccacactt ttttggcatt gacgcgtcag tttcaccaat tcctaagtcc tcaaagtgat
      901 atggccgatg tggttcacgc gctatggtca gaacacggca atctggtgca gacagcgcag
      961 cgcttgtata ttcaccgtaa ctccctgcaa tacaaacttg ataaatttgc tcagcaatca
     1021 ggactacact tgaagcaatt ggatgattta gcctttgctc atctcttttt gttgaaatat
     1081 taggaaagcc acagggcttt cttttttgct gcctggactg tagctgataa ggagaatgat
     1141 agatcgttca gcttgttttg gctcctgtga ctggcctgtg tttacatggt tttcttggtt
     1201 tatggtccct tacgacttga ctagccctgc tattgtgcaa gttgcccaaa gaagtttggt
     1261 cgctttgtct ctgttttgaa agcgctattt ttgttataat aaaagcatca tataaaagat
     1321 atcagcggtt tctagctttg cagtcagcta gaacgtttgc tggctaaata ggagttatac
     1381 atggttgaat taaatctaaa tcacatctat aaaaaatacc caaatactac tcattatgcc
     1441 gttgaagatt ttgacttgga catcaaagat aaagaattta tcgtctttgt tggaccttca
     1501 ggatgtggta aatcaaccac tcttcgtatg attgcaggtc ttgaggacat ttctgaagga
     1561 gagttaaaaa tcggtggcga agtggtgaat gacaagtctc ctaaagaccg tgatattgcc
     1621 atggtctttc aaaactacgc cctttaccca cacatgaccg tgtatgataa catggccttt
     1681 ggtttgaaat tacgtaagta caaaaaagat gatattgacc gtcgtgtcaa ggaagctgcg
     1741 cagatccttg gtttgaccga atttctagag cgtaaaccgg ctgacctgtc tggtggtcaa
     1801 cgtcagcgtg tggctatggg acgtgctatt gtccgtgatg ctaaggtatt cttgatggac
     1861 gagcctttgt caaacttgga tgcgaagctt cgtgtctcca tgcgtgctga aattgctaaa
     1921 attcaccgtc gtatcggctc aacaactatt tacgttaccc atgaccagac tgaagccatg
     1981 actttggcag atcgtatcgt tatcatgagt gccactaaaa accctcaagg taatggaacg
     2041 attggtaaaa ttgagcaagt aggttcacca caagaattgt acaaccttcc agctaataaa
     2101 ttcgttgccg gctttatcgg aagccctgcc atgaacttct ttgaagtgga agttaaagat
     2161 ggccgcattg tgagtgaaga tggccttgct attgcgattc ctgaaggtca agcaaaaatg
     2221 cttgaagctg ctggttacaa ggggaaaaaa gtcacttttg gtattcgtcc agaagacatt
     2281 tctagcagac aaatcgtgca tgatacttac ccaagtgcaa ctgtcacagc agaagttttg
     2341 gtttctgagt tactgggctc tgagacgatg ctctatgtca agcttggaca aactgagttt
     2401 gcttctcgtg tagatgcgcg tgatttccat agtccagggg agcaagttag cttgaccttt
     2461 aatgtggcta aagggcattt ctttgacaga gataccgaac aagctattcg ttaaggtata
     2521 agcgatattc ataaaaaggt tgggttccag cctttttatc atgtcatttt gcgcaaacga
     2581 ttgcataaaa agagaaaaaa cgttaaaata aaagatgaat gaatgattaa taggagaaga
     2641 ataatgaaga aacactggtg gcataaagct acgatttatc agatttaccc aaggtccttt
     2701 aaagacacca gcggaaatgg tattggtgat ttaaaaggca ttaccagtca actggactac
     2761 ttgcaaaaac ttggcattac agccatttgg ttatctcctg tttaccaaag tccaatggat
     2821 gacaatggtt atgacatctc tgattatgaa gcgattgctg atgtttttgg agacatggca
     2881 gatatggatg agctcttagc agcagctaat gagcgcggca ttaagattat catggacttg
     2941 gtggtcaacc atacgtctga tgagcatgct tggtttgttg aagctaggga aaaccctaat
     3001 agtcctgagc gtgattttta tatttggcga gatgaaccta acgatttgac ttctattttt
     3061 agtggctcag cttgggaata tgataatgca tcaggtcaat attatttgca cctttttagc
     3121 aaacgtcagc cagatctcaa ctgggagaat gaagctttac gtcataaaat ttacgatatg
     3181 atgaatttct ggattgccaa aggcatcggt ggttttcgta tggacgtgat tgacctgatt
     3241 gggaaggttc ctgacttgga aatcacgggt aacgggccgc gcttacacga ttacctcaaa
     3301 gaaatgaatc aagtaacctt tggcaaccat gacgtgatga ctgtcggtga gacttgggga
     3361 gcaacgcctg aaattgctcg ccagtattct cgccctgaaa acaaagagtt atccatggtc
     3421 ttccaatttg agcatgtggg tcttcaacat aaacctgacg ctcccaagtg ggattatgcc
     3481 aaagaattag atgtccctgc tcttaaaacc attttcagca agtggcagac agaattaaag
     3541 ttaggagaag gctggaattc gcttttttgg aataaccatg acttgccacg ggtcttatcc
     3601 atttggggca atgacagcac ttaccgagaa aaatcagcca aagctttagc tattttactg
     3661 catttgatgc gaggaacccc ttacatttac caaggagaag agattggcat gaccaattat
     3721 ccttttaaag acttaaccga ggttaatgat attgagtccc ttaactatgc taaggaagct
     3781 atgggaaatg gagtgtcagc tgcgcgtgtg atggatagca tccgtaaggt aggacgagac
     3841 aatgctagaa cgccgatgca gtggtctaag gacacacatg ctggcttttc tgaagcaaaa
     3901 gagacttggt tgccggtcaa ccccaactac caagacatca atgtggccga tgcccttgct
     3961 gatccagact ctatttttta cacctaccaa aaattgattg ccttgcgtaa ggagcaagac
     4021 tggctagttg aagcggatta tcacctcttg ccaacagctg ataaagtgtt tgcttatcag
     4081 cgtcagcttg gagaagagac gtatgtgatt gttgtcaatg tgtctgatga ggagcaagtc
     4141 tttgccacag acttagcagg tgcacaagtg atcatcgcca atacagatgt ggacacggta
     4201 ttaaagacca agcacttaca accttgggat gccttttgtc tcaaactgaa ggcctagcag
     4261 ttgtatgtga tttgagttta cccaacagcc aagcgagcgg aaactcgttt tggctgttta
     4321 atttttgaga tcctattaag gggtgatagt cattgtttga gctgtgttct aagttgaagt
     4381 gcggcatgtc ttgcaagcgc ttgcgcaatg tgataagata gtcttgtttg aatgttatac
     4441 taaatggagg tcttatgaaa aagaaagtca accaaggatc aaagcgctat caacatctgt
     4501 taaaaaagtg ggggataggt tttgtaatcg ctgcaactgg gactgtcatg ttagggtgca
     4561 cccctagtat cttaacacat caagttgctg ctaaaaccat tgttggacta gcccgcgatg
     4621 aagctcaaca aggagatggc aatgctaaat ctggtgatgg ttttcaatcg tctagtaagg
     4681 aggctaaacc agttttagac agctcgtcag ctaatcctgc tagtattgct gagcatcatt
     4741 tgcgtatgca ttttaaaaca ttgccagctg gtgagtcgct aggaagcttg ggactttggg
     4801 tgtggggaga tgtggatcaa ccttcaaagg attggccaaa tggtgctatc accatgacaa
     4861 aagcgaaaaa agatgactat ggctattatc tagatgtgcc actagcagct aaacaccgcc
     4921 agcaagtgtc ttatctcatt aataataaag ctggagagaa tctttcaaag gaccagcaca
     4981 tctcgcttct tacgccaaaa atgaatgaag tttggacaga cgagaattac catgcgcacg
     5041 cttatcgacc tttgaaaaaa ggttaccttc gaatcaacta ccacaatcaa tcgggacact
     5101 acgataactt agctgtctgg acctttaaag atgtcaaaaa cccaacgacc gactggccaa
     5161 atggacttga cttgtcacat aaagggcctt atggagctta tgttgatgtc cccttaaaag
     5221 aaggagctaa cgaaatcgga tttttaatcc ttgataaaag taagacagga gatgctatta
     5281 aagtgcaacc aaaagattat ctgtttaaag agttagacaa tcatactcaa gtttttgtca
     5341 aagacactga cccaaaagtc tacaacaatc cttattatat tgatcaggtt agtctcaaag
     5401 gagctgaaca aaccacgcca aatgagatta aagccatttt tacgacctta gatgggcttg
     5461 atgaagatgc ggtgaaacaa aacatcaaga tcactgacaa agcagggaaa actgttgcaa
     5521 ttgatgagtt gacacttgac aaggataagt ctgtaatgac attaaagggc gattttaagg
     5581 cgcaaggtgc agtctacacg gttacatttg gagaagttag ccaagtcgct cgccaatcct
     5641 ggcaattaaa agataaactc tatgcttacg atggtgaact tggagctacc ctagctaagg
     5701 atggttctgt tgatttagcg ctatggtctc caagtgctga tactgttaag gttgtcgttt
     5761 acgataaaca agatcagaca aaggtggttg gtcaagctga tttgaccaag tcggacaagg
     5821 gtgtttggag agctcatctg acttctgaca gtgtcaaggg aattagtgat tacacaggct
     5881 actattacct ttatgagatc acgcgcggtc aggaaaaagt catggttttg gatccttacg
     5941 ccaaatctct cgctgcctgg aatgatgcga ctgctactga tgacatcaaa acagcaaaag
     6001 ctgcctttat tgatccaagc aaactaggac caacaggcct tgattttgcc aaaattaaca
     6061 actttaaaaa acgtgaagac gctattatct atgaagcaca tgtgcgagat tttacgtcgg
     6121 ataaggctct agaaggcaag ttaacacacc cttttgggac tttttcagct tttgttgaac
     6181 agctagacta tctcaaagac ttgggggtta cccacgttca attgctaccg gttttgagtt
     6241 atttttatgc caatgagctg gacaagagcc gctcaacagc ctacacgtct tcagacaata
     6301 attacaactg gggttatgac ccacaacact actttgccct ttctggcatg tattcggtaa
     6361 atcctaacga ccctgcttta cgtatcgcag agcttaaaaa ccttgtcaat gagattcaca
     6421 aacgtggtat gggtgttatt tttgatgtgg tttataacca cacggctaga acctatctct
     6481 ttgaagattt ggaacccaac tactatcatt ttatgaatgc tgatggcaca gctagagaga
     6541 gttttggcgg aggtcgtcta ggaacgacac atgccatgag tcgtcgtatc ttggtggatt
     6601 cgattgctta tctgactcgt gaattcaagg tagatggttt tcgtttcgac atgatgggtg
     6661 accatgatgc ggcagctatt gagcaagcct ttaaggcagc caaagccatt aatccaaata
     6721 ccattatgat tggcgaaggc tggcgtacct accaaggtga tgaggggaaa aaagaaattg
     6781 cggcagatca agattggatg aaagcaacca atacggtcgg tgttttctct gatgatatca
     6841 gaaataccct caagtcaggt ttcccaaatg aaggcacagc agcctttatt actggtggcg
     6901 caaaaaatct agaaggactg ttcaaaacga tcaaagcaca gcctagtaac tttgaagcag
     6961 atgccccagg agatgtagtg cagtatattg cagcccatga caacctgacc ttacatgatg
     7021 tcattgccaa atccatcaat aaggatccta aagtggctga agaagagatt cataagcgta
     7081 ttcgtctagg aaataccatg attttaactg ctcaagggac tgcctttatc cattctggtc
     7141 aggaatatgg acgaaccaag cagcttctaa atcccgacta caggacaaag gtgtctgatg
     7201 acaaggtgcc aaataaggcg actctgattg atgctgtagc gaaataccct tacttcatcc
     7261 acgattctta tgattcgtct gatgcggtca atcattttga ctgggcaaag gcaacagatt
     7321 ccatagctca cccgattagc aaccaaacaa aagcctatac acagggacta attgcgttgc
     7381 gtcgctcaac agatgccttt acaaaagcaa ccaaagctga ggtagatcgg gatgtgacct
     7441 tgatcaccca agcaggacaa gatgttattc aacaagagga ccttatcatg ggttaccaaa
     7501 cggtggcatc aaatggagat cgctatgctg tctttgtcaa tgcagacaac aagacccgca
     7561 aggtagtttt acctcaagcc taccgctatt tgctaggagc ccaagtgctt gctgatgctg
     7621 agcaagctgg tgttactgcc attgctaagc ctaagggagt ccagtttacc aaagaaggct
     7681 tgactattga tagcctaact gccctggtcc tcaaagtatc ttcaaaaccg gctgatccct
     7741 ctcagaaaaa gagtcagaca ggcaatcatc aaaccaaaac accagatggc tcaaaagacc
     7801 tagacaaatc attaatgact aggccaaaaa gagctcaaac aaaccaaaag ctcccaaaaa
     7861 cgggtgaagc ctcctcaaaa ggcttattag cagctggagt agctctgctt ttattggcta
     7921 ttagcctgtt gatgaagcgc ccaaaagatt agggtggtgt taatagagac aaaaagattg
     7981 ctgtttgcgg attctttttg acggctataa aagctctgag ctgtgaaaag accttgtgat
     8041 tgagaaaatt gctactttag tgtatcataa gaccaaaaga gaaggagaca tccatgacat
     8101 ttgaagaaat cgttgcgaat tttataccgt cgagtgttgc agaagtcaca tcagctattg
     8161 ctagtggcaa agacatgatt gtctttttag gacgttcgag ctgtccttat tgtcgccgct
     8221 ttgctcctaa gttggcacaa gtggcaacag ataatcaaaa agacgtctat tttgtggata
     8281 gcgaaaacgc tgctgacgct gctgagcttg ctgcctttcg tgagaactac caattagtga
     8341 cagttccagc tttgctggtt agttacgatc agcaccaaag ggctgtatgt gattcaagtt
     8401 tgacaccaga tgacatctta gcttttttga ctcgtgagta aagcactgcg cttagcgtct
     8461 cgtttaaaga agaaatcagg taacgtctag tcttgctata ccgcttgatt tttgatatag
     8521 taagacatga cttttataga ataaaaagga gacattatgg gatttccaac aattttaatg
     8581 tttgtagtga tgcttggatt gatttggttt atgcaacgtc aacaaaaaaa acaagcgcaa
     8641 gaacgccaaa atcagttaaa cgctattgaa aaaggagacg aagtcgtaac gattggtggc
     8701 atgtttgcga ttgttgacga agtcgataca acagctaaaa agattgtgtt agatgtggat
     8761 ggcgtctttt tgacatttga gctatcagct attaagcgta ttgtaacaaa agctaccaca
     8821 gaaacgacac tggttgaaac agaagaaacc agtgttgtta atgagcctgc tgttgagaca
     8881 gtagatagca ctgacagtgc cattgaaagc cattaaggcg gggaaagttt ggatgaaata
     8941 tgccaaactt ttttgttttg atccctggac tgaaacagtt attctgatgc taacaggttc
     9001 atgaaaagct gacacatcta tcaaaggata ggtgaacatt ttgtgaagat aagatctaat
     9061 tgataaaaaa caagccaaaa ggcgtgaaat atgttaaaat atgagataag tatgcttatc
     9121 aataaggaaa aaacatgttt ggattaaaag cgaaatcaac aaaaaaagtt ttggggagta
     9181 tcccaaagca tattggaatt atcatggatg gtaatggacg ttgggccaaa aaaagactaa
     9241 aacctagagt gtttgggcat aaagctggga tggatgcttt acaagaagta acgattactg
     9301 catctgagtt gggggttaaa gttctgacag tctatgcctt ttcgacagaa aactggtcca
     9361 gaccccaaga tgaggtgtct tttatcatga atttaccagt aaccttcttt gacaaatatg
     9421 tgcctgtgct tcatgagaac aatgtaaaga ttcaaatgat tggagagact agtcgtttac
     9481 ctgaagatac cttggcagcg ctaaatgcag ccatcgacaa aacaaaacgt aatacgggtt
     9541 taattttgaa ttttgctctc aattatggag gacgtgctga gattaccagt gctgttcgct
     9601 ttattgctca agatgtctta gatgccaaat taaatccagg agatattacg gaggatttga
     9661 ttgctaatta tttgatgaca gaccatctgc catatctcta ccgagacccc gatttgatta
     9721 ttcgcaccag tggtgagctg cgtttgagca atttcttgcc ttggcaatca gcttacagtg
     9781 aattttattt cacaccagtc ttgtggcctg attttaaaaa ggcagagcta ctgaaggcca
     9841 ttgcggatta taaccgccgc caacgtcgtt ttggcaaggt ctaaaaggag atagttatga
     9901 aagaacgtgt tgtttggggg ggagtagcag tagctatttt cctacctttt ctcataatag
     9961 gaaacctacc attccaatta tttgtaggcg ttttggccat gattggggta tcagaattac
    10021 tgaaaatgaa aagattagaa gtcttttctt ttgaaggtgt ttttgccatg ttagccgcct
    10081 ttgtattggc agttccgatg gaccattacc tcactttttt accaattgat gcgaatgttg
    10141 ctttttatag cttaatggtt tttttcattt tggcaggtac tgttttaaat agcagagctt
    10201 attcatttga tgatgcggcc tttccgattg caactagttt ttatgttggg attggttttc
    10261 aacatttgat caatgctcgc ctgtcaggaa ttgacaaggt ctttttggcc ttatttatcg
    10321 tttgggccac tgatattgga gcatatctga ttgggcgtca gtttggcagg cgcaaactat
    10381 tgcctacagt ttctccaaac aaaaccattg aaggtagttt aggagggatc gcttgtgcag
    10441 tccttgtgtc ttttatcttt atgatgatcg ataggtctgt ttatgcacca catcactttt
    10501 tgaccatgtt ggttttggtc gctctttttt ccatttttgc ccaatttggt gatttggttg
    10561 aaagtgctct caaacgtcac tttggtgtca aagattcagg taagcttatc ccgggacatg
    10621 gtggtatttt agaccgcttt gattccatga tttttgtttt tcccatcatg catctctttg
    10681 gtttatttta atcttaagag gtaatgtttg gagtcgcaac aacgcgacag aactcaaaaa
    10741 agcaatagac tgaaaggaaa tctatgttag gaataataac ctttattatt atttttggta
    10801 ttttagtgat tgtccatgaa tttggacatt tctattttgc taaaaaatca ggcattctag
    10861 taagagaatt tgccattgga atgggcccta aaattttttc tcatgttgac caaggaggaa
    10921 ctctttatac cttgaggatg ttacccttgg gcggttatgt gcgaatggct ggttggggtg
    10981 atgacaaaac tgagatcaaa acaggtactc cagcaagttt aacccttaat gagcaaggtt
    11041 ttgttaagcg catcaacttg tctcaaagta agttagaccc aacgagtctc ccaatgcatg
    11101 ttacaggcta tgacttagaa gatcagctga gtattactgg cttggtttta gaagaaacca
    11161 agacatataa ggtcgctcac gatgctacta ttgttgaaga agacggtact gagataagaa
    11221 ttgctccgct tgatgttcaa tatcaaaatg ctagtattgg cggacgttta atcaccaatt
    11281 ttgcaggtcc catgaataat tttattttag ggattgtggt ttttatcctc ttggtctttt
    11341 tacaaggtgg gatgccagat tttagtagca atcatgtccg tgttcaagaa aatggagcag
    11401 cagctaaggc tggtcttcga gataatgacc aaattgtcgc aattaatggt tacaaggtga
    11461 ctagctggaa tgatctcact gaggctgtag accttgcaac acgggatcta ggcccgtcac
    11521 agaccattaa ggtcacctac aagtcacatc agcgcttaaa aacggtggct gtgaaaccac
    11581 aaaagcatgc aaagacatac acgataggag ttaaggcgag tctgaaaaca ggatttaagg
    11641 ataagctctt aggcggtcta gaattagctt ggagtggggc gtttactatt ttgaatactt
    11701 tgaaaggatt gatcactggc tttagtctca ataaattagg tggacctgtt gccatgtatg
    11761 acatgtccaa tcaggctgct caaaacggct tagagtcagt cttatctctt atggcaatgc
    11821 tttcgatcaa tttagggatc tttaacctga ttccgattcc tgcacttgat gggggaaaaa
    11881 tcttgatgaa tatcattgaa gccattcgtc gcaagcctat caagcaagaa acagaggcct
    11941 atatcaccct agctggggtt gctatcatgg tcgtattgat gattgctgtg acatggaatg
    12001 atatcatgcg cgtctttttc taatcaagat aggtcatctt aagctgacct tatcatgtta
    12061 aagatcgaaa aagacctcaa ccctaaggtt tgcaagggtt gaagtcttac tcaaaatgaa
    12121 aggaatacac attcaaatta ttgaataatc ccgcttcact cttacttgat gtttccagaa
    12181 gggtttgagg ataaggggat gtattaaaac ggaataatct atgaaacaaa gtaaactgct
    12241 tatcccaacc ttgcgcgaaa tgccaagtga tgcccaggtt atcagccacg cgcttatggt
    12301 gcgtgccggt tatgtgcgcc aagtttctgc tggtatctat gcttatttac cactggcaaa
    12361 tcgtaccatt gagaaattca agaccatcat gcgtgaagag tttgaaaaga tcggtgctgt
    12421 tgaaatgttg gcaccagctc ttttgacagc tgatctctgg cgtgaatcag gccgttatga
    12481 gacctatgga gaggacctct ataagcttaa aaaccgtgat aactcagact ttatcttggg
    12541 tccgacccac gaagaaacct ttacgacttt ggtgcgtgat gcggtcaaat cttacaagca
    12601 attgccctta aacctttatc aaattcagtc taagtatcgt gatgaaaaac gtccgcgtaa
    12661 cggcttgctt cgtacccgtg agtttatcat gaaagacggc tatagtttcc atcacaacta
    12721 tgaagattta gatgtgacct acgaagatta tcgtcaagct tacgaagcta tttttaccag
    12781 agctggtcta gatttcaaag ggattattgg agatggcggt gccatggggg ggaaagattc
    12841 ccaagaattt atggccatta caccagctcg tacggacctt gatcgctggg tggttcttga
    12901 caagtctatt gcgtcaatgg atgatattcc aaaagaggtc ttagaagaga ttaaggcaga
    12961 attggctgct tggatgattt caggtgaaga tactattgct tattcaacag aatcaagcta
    13021 cgctgccaac cttgagatgg caactaacga atacaaaccg tcctcaaaag tagctgctga
    13081 agatgccttg gcagaagttg agacaccaca ttgcaaaacg attgatgaag tggctgcttt
    13141 tctttcagta gatgaaacac aaaccatcaa aaccttgctt tttgtggcag ataatgaacc
    13201 tgttgttgct ttgctagttg gaaatgacca tatcaatacc gttaaattaa aaaactatct
    13261 agctgctgat tttttagagc cagctagtga agaagaagcc cgtgctttct ttggtgcagg
    13321 ttttggctca cttgggcctg tcaacttggc acaaggtagt cgcattgtgg ctgaccgcaa
    13381 agtgcaaaac cttaccaatg ccgttgcagg agctaacaag gatggtttcc atgtgacagg
    13441 agtcaatcca ggacgtgact tccaggctga atatgtagat attcgtgaag tcaaagaagg
    13501 ggaaatgtct ccggatggtc atggtgttct ccagtttgcg cgtggtatcg aagtcggtca
    13561 tatcttcaag ttgggcactc gttattcaga cagcatggga gcaacgattc ttgatgaaaa
    13621 tggcagaaca gttccaattg tgatgggttg ttatggtatc ggggttagcc gcattttgtc
    13681 tgctgtcatt gaacagcatg cccgtctctt tgtgaacaag acaccaaaag gcgattaccg
    13741 ttatgcttgg ggtattaact tccctaaaga attagcccca tttgacgtgc atttgattac
    13801 agttaatgtc aaagatcaag tggcccaaga cttgacggcg aagttagaag ctgacttgat
    13861 ggctaaaggg tatgatgtct tgacagatga ccgtaatgaa cgcgtcggtt cgaaattctc
    13921 tgatagcgat ttgattggtt tgccaattcg tgtcactgtt ggtaaaaaag ccgctgaagg
    13981 tatcgtggaa attaaaatca aggcaacagg tgacagcatt gaagttaatg cagaaaacct
    14041 catcgaaacc cttgaaattt taacaaaaga acactaaaaa agaaatgaag aggctgcctg
    14101 tttagagtag cctctttttg ctgtgaactt gtctgataca gagtggcttt tgctttacca
    14161 aaagcgcttg gcgacaaaag attggtaaaa aaatgacctc agctgagcta tcacttggaa
    14221 taaattagcc aaagaacgtc agaagaaagc cctgcttttc tttccatcta aaactcgaaa
    14281 atcatcttga attgtgataa aataaagcct agtaaaatca ggagaaaatt atgtcagatt
    14341 tattcgctaa attgatggac cagatagaaa tgccacttga catgagacgt tcaagtgcct
    14401 tttcatctgc tgatattatc gaggtaaagg tgcattcggt gtcacgcttg tgggaatttc
    14461 attttgcctt tgcggcggtt ttaccgattg caacttatcg tgaattacat gatcgtttga
    14521 taagaacttt tgaggcggct gacattaagg taacctttga tatccaagct gctcaggtgg
    14581 attattcaga tgatctgctt caagcttatt accaagaagc ttttgagcat gcaccgtgta
    14641 atagtgctag ttttaaatct tctttctcaa agctcaaagt gacttatgag gatgacaaac
    14701 tcattattgc agcgccaggt tttgtgaata acgatcattt tagaaacaat catctgccta
    14761 atctggtcaa gcaatttgaa gcctttggct ttggcacctt gaccatagat atggtgtcag
    14821 atcaggagat gactgagcat ttgaccaagg attttgtttc cagtcgtcag gctcttgtga
    14881 aaaaggctgt gcaggataat ttggaagccc aaaaatccct tgaagccatg atgccaccag
    14941 ttgaggaagc cacacctgct cctaagtttg actacaagga acgagcagct aagcgtcagg
    15001 cagggtttga aaaagcgacc atcacaccaa tgattgagat tgagaccgaa gaaaaccgga
    15061 ttgtctttga ggggatggtt tttgatgtgg agcgtaaaac gactagaaca ggtcgccata
    15121 tcatcaactt taaaatgaca gactatacct cctcgtttgc tctccaaaaa tgggctaaag
    15181 acgatgagga gctccgtaaa tttgatatga ttgctaaggg ggcttggtta cgggtacaag
    15241 gtaatattga gaccaatcct tttacgaaga gtctcaccat gaatgttcag caggtcaaag
    15301 aaattgtcca tcatgagcgc aaagacctga tgccagaagg acaaaaacgg gtcgaacttc
    15361 atgcccacac caatatgtct accatggatg ccttaccgac agtagaaagc ttgattgata
    15421 cggcagccaa gtggggacac aaggcggttg ctattaccga tcatgctaat gtgcaaagtt
    15481 ttcctcatgg ctaccatagg gctcgcaaag ctggaattaa ggctattttt ggtctagaag
    15541 ccaatattgt tgaggacaag gtgcctattt cttatgatcc tgttgatatg gatttgcacg
    15601 aagctaccta tgtggtcttt gacgtggaaa ccacaggtct atctgctatg aataatgacc
    15661 tgattcagat tgcggcttcc aaaatgttta aaggaaatat tgtagagcag tttgatgaat
    15721 tcattgatcc tgggcatcct ctttctgcct ttaccaccga attgacaggg attaccgata
    15781 agcatttgca gggcgccaag ccattggtta ccgtcctaaa agcttttcag gacttttgca
    15841 aagatagtat tttagttgct cacaacgcca gttttgacgt gggctttatg aacgccaatt
    15901 atgaacgcca tgacttgccc aaaatcacac agcctgtgat tgatacctta gaattcgcaa
    15961 gaaatttata tcctgaatac aaacgtcacg gtttgggacc actcaccaag cgtttccaag
    16021 tgagtctaga ccaccatcat atggccaatt acgacgcgga agccacagga cgtcttttgt
    16081 ttatttttct aaaagatgcc agagaaaagc atggcatcaa aaaccttttg caactcaata
    16141 cagatttggt agctgaggac tcttacaaaa aagcgcggat taagcatgcg actatctatg
    16201 tgcaaaatca ggttggtctt aaaaatatgt ttaagttggt cagcctttcc aatatcaaat
    16261 attttgaagg ggtgccgcgt attccaagaa ccgtcttaga tgctcacaga gagggtttgt
    16321 tactaggaac agcttgttcg gatggtgaag ttttcgatgc cgttctgact aaaggaattg
    16381 atgcggcggt tgatttggct aagtattatg attttatcga aatcatgcca ccagccattt
    16441 accagccatt ggttgtccgt gaattaatca aagaccaagc aggtattgag caggtgattc
    16501 gtgacctcat tgaagtaggg aaacgagcta ataaacttgt acttgccact ggaaatgtgc
    16561 attacctaga gcctgaagaa gagatttacc gtgaaattat tgtgcgtagt ctcggtcagg
    16621 gtgccatgat taatagaaca atcggccgtg gggaaggggc ccaacctgct cccctaccta
    16681 aagcgcactt tagaacaacc aatgaaatgc tggatgagtt tgcctttctt ggaaaagacc
    16741 tcgcttatca agtggttgta gaaaacactc aggattttgc ggaccgtatt gaggaagtgg
    16801 aagtggttaa gggcgatctt tacaccccgt atattgacaa ggccgaagag acggttgccg
    16861 aattaaccta tcaaaaagcc tttgaaattt atggtaatcc tctcccagat attattgatt
    16921 tacgcattga aaaagagtta acctctatct tggggaacgg ttttgccgtg atttacctag
    16981 cgtcacaaat gcttgttaac cggtcaaatg agcgaggcta cctagttggt tctaggggat
    17041 ctgtagggtc tagttttgtg gccaccatga ttgggattac cgaggttaat cctatgccgc
    17101 ctcactacgt ttgcccgtct tgccaacatt ctgaatttat cacagatggg tcagttggat
    17161 cgggctatga tttgcctaat aaaccttgtc caaaatgtgg caccccttat caaaaagacg
    17221 gccaagatat tccctttgag accttccttg gatttgacgg ggataaggtg cccgatattg
    17281 atttgaactt ctctggtgat gaccaaccca gtgcccattt ggatgtccga gatatttttg
    17341 gtgctgagta tgcttttcgt gctggaaccg taggtaccgt agcagaaaaa acagcctatg
    17401 gatttgtcaa aggctatgaa cgcgactatg gcaagttcta tcgtgatgct gaggtggatc
    17461 gtctagcagc aggtgctgct ggtgtgaaac gaacgactgg gcagcaccct ggggggattg
    17521 ttgttattcc taattacatg gatgtttatg attttacccc cgtgcaatat ccagccgatg
    17581 atgtaacggc ttcttggcag acaactcact ttaacttcca tgatattgat gaaaacgtct
    17641 tgaaacttga tatcctaggg catgatgatc cgaccatgat tcgtaaactt caggatttat
    17701 cgggcattga tcctattact attcctgctg atgatccggg agttatggct ctcttttctg
    17761 ggacagaggt tttgggcgtt accccggaac aaattgggac accgactggt atgctaggca
    17821 ttccagaatt tggaaccaac tttgttcgcg gcatggttaa tgagacgcat ccgaccactt
    17881 ttgcggagct tttgcagttg tctggactat ctcatggaac cgatgtttgg cttggtaatg
    17941 cacaagattt gattaaagaa ggcattgcga ccctaaaaac cgttatcggt tgtcgtgacg
    18001 acatcatggt ttacctcatg cacgctggct tagaaccgaa aatggccttt accattatgg
    18061 agcgtgtgcg taagggcctc tggctaaaaa tttctgagga agaacgtaat ggctatattg
    18121 atgccatgcg agaaaacaat gtgcccgact ggtacattga atcgtgtgga aaaatcaagt
    18181 acatgttccc taaagcccat gcggcggcct atgtcttgat ggcccttcgg gtagcttatt
    18241 ttaaggtgca ccaccccatt atgtattatt gtgcttattt ctctattcgt gcgaaggctt
    18301 ttgaattaaa aaccatgagt ggtggcttag atgctgttaa agcaagaatg gaagatatta
    18361 ctataaaacg taaaaataat gaagccacca atgtggaaaa tgacctcttt acaaccttgg
    18421 agattgtcaa cgaaatgtta gaacgcggct ttaagtttgg caaattagac ctttacaaaa
    18481 gtgatgctat agaattccaa atcaaaggag atacccttat ccctccattt atagcgttag
    18541 aaggtctggg tgaaaacgtg gccaagcaaa tcgttaaagc tcgtcaagaa ggcgaattcc
    18601 tctctaaaat ggaattgcgt aaacgaggcg gggcatcgtc aacgctcgtt gagaaaatgg
    18661 atgagatggg tattttagga aatatgccag aagataatca attaagtctt tttgatgact
    18721 ttttctagat tatcatgatg gtagtcatat gcaaagtcat aaaagaagag ggacaagatc
    18781 actttctaat ctctctgata ctaaactgtg acataccgta taaagcgagg aaatcaaatg
    18841 agtcatttag ataagaacac cgctttgaaa gccatggtgg tgtttcgtaa agctcagcga
    18901 acgctagatg cttttggggc tgatattttt aaaaaggcag atttaacagc cacgcagttc
    18961 agtgtgcttg aagtactata tactaaaggc tgtatgcgaa tcaatcactt gattgattct
    19021 ttgttagcga cttcggggaa tatgactgtt gtactaaata atatggaacg taatggctgg
    19081 attagcaagt gcaaagacaa aactgataaa agagcctatg tggttacctt aacagataag
    19141 ggtactcgct tgattgaagc tgttttgcct aaacacgtcg cacgggtgga agaagccttt
    19201 gctgttttga ctgaaaaaga acaactttgt ctgattgaac ttctcaaaaa gttcaaacaa
    19261 ttataaacaa atctctttac agctcttctt
//
LOCUS       DAUELZ010000027        19240 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.027, whole genome shotgun sequence.
ACCESSION   DAUELZ010000027 DAUELZ010000000
VERSION     DAUELZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19240
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.027"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..856
                     /locus_tag="VHI09_000717"
     CDS             <1..856
                     /locus_tag="VHI09_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ6120609.1"
                     /translation="WFHKGDHKEVFAYSAQVACDKYGWALAYSVEAGNIHDSQAFPAL
                     FAKLEPLKPQFIIADSGYNIPSIAKFLIDKEITPVLPYTRPRGKKDLLRPNEFIYDEH
                     FDCVICPEHQALTYRTTTREGYREYKSDPAICANCPLLSVCTTSKNHQKVVTRHIWKD
                     YLEQCEDIRHQRGMKELYQHRKETIERLFGTAKEYHNLRYTREKGKSKMEDKVGLTLA
                     CLNLKKLVKRMAGEPFYFVQMRWFKHGNSHFYLKTLKKTSTKSMFVFNLGFVKPFFYA
                     IIVKILRK"
     gene            949..1731
                     /locus_tag="VHI09_000718"
     CDS             949..1731
                     /locus_tag="VHI09_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120610.1"
                     /translation="MTTISAEDLTIAYGQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGIVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFLIKPVLVDDPIHNCPIVLTYQLQ"
     gene            1757..2689
                     /locus_tag="VHI09_000719"
     CDS             1757..2689
                     /locus_tag="VHI09_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ6120611.1"
                     /translation="MKKLTLLLTLCLTTTTLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGHIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            2679..3731
                     /locus_tag="VHI09_000720"
     CDS             2679..3731
                     /locus_tag="VHI09_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ6120612.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLANAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            3692..4729
                     /locus_tag="VHI09_000721"
     CDS             3692..4729
                     /locus_tag="VHI09_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ6120613.1"
                     /translation="MLFMVNKKRRPLLKSQRHLLRLYTSLILLLVSLMGLALSLGESH
                     LSFLDFVHVFLGKSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAVVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTMLSSLMVALVGHINHYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKLTIPISILIGMILLLVADTVGRVYLVGSNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(4774..5427)
                     /locus_tag="VHI09_000722"
     CDS             complement(4774..5427)
                     /locus_tag="VHI09_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEQ6120614.1"
                     /translation="MITIFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYICRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     HLTNQTNHVMIKEKTNITRDDLTLANYFYRLKRGEKPSAEQMDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(5503..6438)
                     /locus_tag="VHI09_000723"
     CDS             complement(5503..6438)
                     /locus_tag="VHI09_000723"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEQ6120615.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            complement(6569..7360)
                     /gene="pflA"
                     /locus_tag="VHI09_000724"
     CDS             complement(6569..7360)
                     /gene="pflA"
                     /locus_tag="VHI09_000724"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEQ6120616.1"
                     /translation="MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPSKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            complement(7438..8772)
                     /locus_tag="VHI09_000725"
     CDS             complement(7438..8772)
                     /locus_tag="VHI09_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEQ6120617.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            complement(8905..9459)
                     /locus_tag="VHI09_000726"
     CDS             complement(8905..9459)
                     /locus_tag="VHI09_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ6120618.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLLNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADV"
     gene            complement(9498..10418)
                     /locus_tag="VHI09_000727"
     CDS             complement(9498..10418)
                     /locus_tag="VHI09_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEQ6120619.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKDTVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            complement(10711..11364)
                     /locus_tag="VHI09_000728"
     CDS             complement(10711..11364)
                     /locus_tag="VHI09_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ6120620.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQILLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILVIGLSRIYLGVHYPSDILAGFVLGFGILQFIYPFYKQKRFEWRFLLKQD"
     gene            complement(11366..11929)
                     /locus_tag="VHI09_000729"
     CDS             complement(11366..11929)
                     /locus_tag="VHI09_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ6120621.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     regulatory      complement(12008..12131)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(12240..12788)
                     /locus_tag="VHI09_000730"
     CDS             complement(12240..12788)
                     /locus_tag="VHI09_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEQ6120622.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHTYEHDKLK"
     gene            complement(12966..13229)
                     /gene="yidD"
                     /locus_tag="VHI09_000731"
     CDS             complement(12966..13229)
                     /gene="yidD"
                     /locus_tag="VHI09_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEQ6120623.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            complement(13226..13948)
                     /locus_tag="VHI09_000732"
     CDS             complement(13226..13948)
                     /locus_tag="VHI09_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ6120624.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            complement(14069..14620)
                     /locus_tag="VHI09_000733"
     CDS             complement(14069..14620)
                     /locus_tag="VHI09_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEQ6120625.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            complement(14617..15321)
                     /locus_tag="VHI09_000734"
     CDS             complement(14617..15321)
                     /locus_tag="VHI09_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEQ6120626.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKNNHTV
                     IERDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNEVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            complement(15321..16067)
                     /gene="xerD"
                     /locus_tag="VHI09_000735"
     CDS             complement(15321..16067)
                     /gene="xerD"
                     /locus_tag="VHI09_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEQ6120627.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLVGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLIGKEVYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPVTLEKYYKS"
     gene            complement(16064..16525)
                     /locus_tag="VHI09_000736"
     CDS             complement(16064..16525)
                     /locus_tag="VHI09_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ6120628.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDYPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            complement(16522..17043)
                     /locus_tag="VHI09_000737"
     CDS             complement(16522..17043)
                     /locus_tag="VHI09_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEQ6120629.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            complement(17022..18008)
                     /locus_tag="VHI09_000738"
     CDS             complement(17022..18008)
                     /locus_tag="VHI09_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEQ6120630.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQRKIKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDIILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            complement(18005..18799)
                     /gene="racE"
                     /locus_tag="VHI09_000739"
     CDS             complement(18005..18799)
                     /gene="racE"
                     /locus_tag="VHI09_000739"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEQ6120631.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSVQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLAPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVEHRFFTTANSEIFQEIASIWLKQKINVEHVTL"
     gene            complement(18977..19219)
                     /locus_tag="VHI09_000740"
     CDS             complement(18977..19219)
                     /locus_tag="VHI09_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEQ6120632.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
BASE COUNT         5776 a         3976 c         3376 g         6112 t
ORIGIN      
        1 ctggtttcac aaaggtgatc ataaggaagt cttcgcttat tctgctcagg tggcttgtga
       61 caagtatgga tgggcgcttg cttatagtgt tgaggctggc aatatccatg atagtcaggc
      121 ttttcctgca ctttttgcca agcttgaacc cttgaaacca cagtttatta ttgcggattc
      181 cggctataac attcccagca ttgccaagtt tctgattgat aaggagatca ctcctgtcct
      241 tccttacaca cgaccaagag gaaagaaaga ccttctacgt ccgaatgaat ttatttatga
      301 tgagcacttt gattgtgtca tttgccctga acatcaagcc ttgacctacc gcactacgac
      361 cagagaaggc taccgagaat acaaaagtga ccctgcaatt tgtgccaatt gtcccctgtt
      421 atctgtatgt acgaccagta agaaccacca aaaagttgtc acacggcata tttggaaaga
      481 ctatttggaa cagtgcgagg acattcgtca tcaaagggga atgaaggagc tctaccagca
      541 tcggaaagag acgattgaac gactgtttgg cacagccaag gaataccaca atctccgcta
      601 tacgagagag aaaggcaagt ccaaaatgga agataaggtt ggactgactt tggcatgctt
      661 aaatctcaaa aaactggtca aaaggatggc gggagagcct ttttattttg ttcagatgag
      721 gtggtttaag cacggaaaca gccattttta tctaaaaaca ctaaaaaaga caagcaccaa
      781 atcgatgttt gtcttcaatc tgggctttgt taagcctttt ttctatgcta taatagtgaa
      841 gattttgaga aaataagcta cttctcaaat attttaaaat gggcagatga acactttaat
      901 gtgcactttc atcaaccctt tgactctttt tagaaaggag gcaaccatat gacaacaatt
      961 agtgctgaag acctcacaat tgcctatgga cagcgtacca tcatcgataa attatcgttt
     1021 tatattcctg aaggtaaaat tacgaccatt attggcgcca atggctgtgg gaagtctagt
     1081 ttattaaaag cactgacaag attattacca ccaaaacagg gaattgtgta tttaaatggg
     1141 caaaacattg ccacattaga aactaaagag gttgctaaaa aactagctct tttacctcaa
     1201 gttcaagaag caactaacgg cataactgtt tacgaattgg tttcttacgg acgttttccc
     1261 catcaatctt actttggaaa tcttagtcca gctgacaaaa aagccattca ttgggctatg
     1321 caagccacca atgtcatggc ctatgctgat caacctgttg atgctctttc tggggggcaa
     1381 cgtcaaaggg tttggcttgc aatggcatta gcccaaggca ctgatactat ttttttagat
     1441 gagccaacta cttatttaga tttaaatcat caactagaaa tcctagagct cgttaaatcc
     1501 ctaaataagg atgccggcaa gactattgtt atggttcttc acgacctcaa tctttcagcc
     1561 cgctattcgg atcatctgat tgctatgaaa catggcaaga tccattatac tggtactata
     1621 gcagatgtta tgacttcacc tatcattcaa gacatcttcc taatcaaacc ggtactggtt
     1681 gatgatccta tccacaactg tcctatagtg ctgacttatc agcttcaata actttattct
     1741 aggagaatta gcatacatga aaaaattaac tctactatta acactctgtt tgacaaccac
     1801 taccttaatc gcctgtggta atcaagcaac taatcattct aacaccgcct caaaaagttt
     1861 atcgcctatg cctcaaattg ctggtgtgac ttattacgga gatattccta aacagcctaa
     1921 gcgagtagtc agtctggctt caacttacac tggttacctc aaaaaattag atatgaatct
     1981 agtcggggtt acttcttatg acaagaaaaa ccccatctta gcaaagactg taaaaaaagc
     2041 aaagcaagtt gctgcaactg atttagaagc cattacaacg cttaagcctg acctcattgt
     2101 tgttggctct acagaagaaa atattaaaca attggcagaa attgcgcctg ttatttcgat
     2161 tgaataccgc aaacgtgatt atttacaagt gttatctgac ttcggccaca tctttaacaa
     2221 agaaggcaaa gccaagaaat ggttaaaaga ttggaaaact aaaacagcag cttatgaaaa
     2281 agaagtcaag gccgttacag gtgacaaagc aacctttact attatggggc tttatgaaaa
     2341 agacgtttac ctttttggta aagactgggg acgtggcgga gaggttatcc atcaagcttt
     2401 ccattatgat gctcctgaaa aagtgaagac tgaagttttt aaacaaggtt acctgtcatt
     2461 gtcgcaggaa gtcttgccag actatattgg tgattatgtc gtcgttgctg cagaagatga
     2521 taagactggt tctgctctat atgaaagcaa gctatggcaa tccattccag ctgtgaaaaa
     2581 acaccatgtg atcaaagtca atgccaatgt cttttacttt actgatcctc tttctttaga
     2641 atatcaatta gagactttac gagaggcaat cttatcaagt gaaaactaag catccctttt
     2701 tgtgtctcga gcgtttttgc ttaaaaaaaa cgcagataat cactaagtca catatctttt
     2761 ggcttgtctt tgtgctactt agttttagct tatgtgttgc tatttactgt catctgcgct
     2821 ttggagccgt tgctttatca catcaggatc ttaactctat tttatttgga aagcaaaacg
     2881 gccataaagc caatgtttta ctagctatac gattaccgag actttttggt gctaccttaa
     2941 ctggctctgc gttagctgtt tctggaacta tcatgcaagc aatcacacgt aatcctatcg
     3001 ctgagcctgg ccttttaggc attaatgctg gagcaggact agccttggta ctggcttatg
     3061 cgtttgtacc tcacttgcac tattccctca ttattctgct ttcgttgtta gggtctagcc
     3121 tggcagcaac cttagtcttt ggcctttctt atcaatctgg caaaggctac catcagctcc
     3181 gccttgtctt agcaggagct atggtttcca tactgctatc agcattaggt caaggtatta
     3241 ccaattacta tcatctggca aatgctgtta tcggctggca agcaggaggt cttgtcgggg
     3301 tcaattggca aatgatcggc tatattgctc ctcttattat tcttagtctt tgtttagccc
     3361 agctattatc ttatcacctg accgtcctta gtctaagtga gtctcaggca aaagctcttg
     3421 ggcaaaagac caacttgatc agtgcagtct ttatgatttt ggttcttatt ttatcatcag
     3481 cagctgtagc cattgctgga agcatttctt ttatcggctt agtcattcct catcttatga
     3541 aacactttac acctcatcat taccgatacc ttttgccgct ttgtgcagtt tctggcgcta
     3601 gtttcatggt atgggttgat attgcttgtc gtaatctcaa tccgccttat gaaacccctc
     3661 ttggggcatt ggttagctta attggttttc catgcttttt atggttaata agaagaggag
     3721 gccgctattg aaatctcaaa gacacctttt aaggttatat actagcctga tcctgctact
     3781 tgtaagtctt atgggactag ctttgtccct tggagaatct catctatctt ttttggactt
     3841 tgtccatgtg tttttgggaa aatctagcca tgccatatct ttcatcgtta ttaacattcg
     3901 tctgccaaga attctagctg cttgtctagg aggcggttct cttgctttat cagggctatt
     3961 attacaacgt ttaacacgaa atcccttagc tgactctggt gtattaggta ttactatagg
     4021 agcagggatc tctttggcta ttgtggtctc cttttctttc tttgagcaag ctcatatcag
     4081 ccattacttg cctttatttg ccatgcttgg tgctgttgta acaaccttta gcgtttattg
     4141 gctatctttg acaaaacaag gacaaattga tcctacacgg ttaattctta ctggtgtagc
     4201 tgttactacc atgctttcaa gtctaatggt tgctcttgtt ggtcatatta atcactacaa
     4261 agtcgatttg gtcatcaatt ggcttagcgg ccaattaata ggagacgact ggcctactct
     4321 gagtgtcatc gctcctttat tattgtgttt ttggctattg acttatagtc aagcccattt
     4381 ccttaatatt atgggattag ctgataatac tgctattgga ctaggactcc ctttaaaccg
     4441 taagcgtcgt ttgatacttg tcttagcagc tggcttaggt gctttgagtg ttcttttggt
     4501 tggtaatatt agttttatcg gcttaatagc tggacacttt agtacatact tagtagggag
     4561 taatcataaa ttaacaatac ccattagcat tttaatcgga atgatcctac ttcttgttgc
     4621 agacacagtc ggacgagtgt atctggttgg cagtaatatc cagacaggga ttttagtgtc
     4681 gcttatcgga gccccttatt ttctttactt aatggcaaaa acaaaataaa agcactcaaa
     4741 aagctaagaa cagtccttgt tcctagcttt tttctatggc gctataaagg ttaaactttc
     4801 tttatccaag gtcattagca gctggtatgt tgtaggctcc acttgtttta ggtaaccaag
     4861 tgtaaccaga gcttccacaa atatatcagg acgtttttgc atcacaaggt ctttgcgcgt
     4921 gaatttaagc aaaaaagttg tcatatattt aagggcatat tcctgattca catcccccaa
     4981 gaggtcatac aagtccatct gctcagctga tggcttctca cctcttttga gacggtaaaa
     5041 ataattagcc agggtcaaat cgtctctcgt gatgttggtc ttttccttga tcatcacgtg
     5101 attggtttgg tttgttagat gagtctcaaa aacaaccgct aaaatatttt catacatagc
     5161 tgaacaggtg tccacaaaaa gcatagtgtc taaggccagc tgctgatcag aactcaccaa
     5221 aggaagattg atgccataac gcttattttc acggcaaata tagccagctt ggacatagct
     5281 ttcgatggcc ttgtcaatac ctgtcacatt aggaaaggct tttttgatct ctcgtaatat
     5341 aacgtgatca tgctggtcga gataattgat aaggtcttga aagaaaggtt gtctggtgag
     5401 tttatctgaa tgaaatattg ttatcatacc ttctattata gcatagaaga gtgaaaaaag
     5461 gacttggtca aaaaaaccaa gtccttttaa agcgtctttt cattaagctc caaaactttc
     5521 tgtcaattgt ggaaccactt gttttttacg agaaacggca cctgctaaga aggcatgatt
     5581 gttgtcaagg gtgaagttaa aggctgcttc aaccttgtcc atgtttgctc caatagctag
     5641 aatttcagag tttgagttaa caatatccgt gatcatcaaa acaaagtctg aataaccttc
     5701 tgcagccatg gcatctttaa tggctgcttc aatagcttct tgacgttcta agacttctgc
     5761 aatgtcaaca gtgttcactt gtgccacgcg tacagcattg ccatttaatt caaatgtttt
     5821 ggcatcaata ccaatcaatt ctacttctga tttgcttgca aggtttgtac ctgcttttaa
     5881 aagagccatt ccatagtctt ccaagttcac ttctgctagc tctgccaatt cttcagcgac
     5941 aaggtgatca gacacatgag ttgttggaga tttcaacaac aaggtatctg aaatcaaacc
     6001 agacaagagc atccctgcaa tcgcttttgg cacctcaatg ccattttctt taaacatacg
     6061 gtaaacaata gatgaggctg atcctactgg ttccacacgc atgtataaag gatttgcagt
     6121 ttcaaaatta gccacacggt ggtgatcaac cacaccatat acctcaacct cacggatatc
     6181 tgcaatggat tgttggaatt cattgtggtc tgtcaaaatc acttgttcag acccttgtgc
     6241 tttagcagac tctaccacac gtggtgcctc aacaccaaaa taatcaaggg caaaagctgt
     6301 ttcttcgtta ggtgttccta aagctacgac ttcagtatcc aaaccaaacg ctttttggga
     6361 taagtaatca aaagcataag atgatgcaat agcatccgta tcaggatttt gatgaccaaa
     6421 aactaaaatt tttgacataa tcattctacc tcattttgtt tattcatttt attttagcaa
     6481 aattaccccc taaaatcaaa ggaagattac aaaagatctg cttataatac tttaaaaagt
     6541 tgaggaaacc tcaaccctat aacagctctt atgactgatg aatccgattc atatattctg
     6601 tgtaagattc ggtctgcatc agatttttag cattttgaac gcgttcctta cttggtggtt
     6661 taacaccttc taactgataa ggaataccta gctcacgcca cttgaattct cccatggtat
     6721 ggtaaggcaa aacttcaaat ttatcgacat ttttcaaggt tttgacaaac tcacccaatc
     6781 gtgtcaaatg atcatcaata tcagtcaagc ctggaaccaa gacatgtcga atccagacag
     6841 gaatctgctt gtcggataaa tacctcgcaa attgcaatat gtttttatta ggctgacgcg
     6901 tcacaatttt atgttgtttt tcatcaattt ctttcaagtc caataaaatc aaatcggtca
     6961 ctgccaataa gttatcaaga acctgatggt attcaggtgt cggtctatag gtaaagccac
     7021 aggtgtctag tgtggtatgg atacctaatt ttttagcttc gatgaataag gcagtgataa
     7081 aatcaatttg aagcatagct tcaccaccag atacggtaat tcctcctttt tttccccaga
     7141 aatgtttgta ctgaagggct tctttcaaga catcattgac tgttctgatt tttgaattgt
     7201 tggtttccat ctcccaagta tccggattat ggcaatattg gcaacgtaat ttacagcctt
     7261 gcaagaaaat gataaaacga atgcctggac catctacaga accaaaactt tctgtcgaat
     7321 gcaccatccc tgttacttgt ccataatctt tctcagtcat tgcatctcct tcattacttg
     7381 taaacgtttt tacattttca ttataacact tttcatcaaa aagactaggc atggagccta
     7441 gtcttcctca ataatctgtt ctatattaga caggattaat tttaatttcg taatacggcc
     7501 atctttgact ttatcgttga ttagaactaa atgtttgtct ttgttatcta tttcgtaggc
     7561 ttctttttgc tcctggcttg gaatggttcc gatacctgtc aaataaaaac cagcaatggt
     7621 atctacatca tctgattcta gttcggtatc aaaatagtca ttaaactcat ttaaagtcat
     7681 ggtaccaaca acaatatagg tattgtctcc aatctcatga acaaattgtt ctgctttatc
     7741 ggtttcatca tcgatttcac cgacgatttc ttcaagcaag tcttccaaag tcacaagtcc
     7801 tgccacacca ccatattcat ctagcaaaat agccatctga ttttgggtat tgcgcagctg
     7861 gcgtaagaga tcatctacaa aaatggtttc gggaacaaaa agaggttctt gtaacatttt
     7921 tcgcatgtta atctgatcaa atccctggcg gaaacctgac tccaagagac gcttagtgtg
     7981 gatgagaccg ataattttat ctttatccac atcataaaca ggaatgcgtg aaaaactttg
     8041 ttttaagatt tcctgaatat tttcaagcgg atcatcgtta atgtcaatca tgaaagcatc
     8101 ggtccttggg accatgactt cacgcgccat catttcatca agtgagaaaa ctccttgcaa
     8161 catctcaatt tcttcagcat caagggtcgc ctcacttttt gataacatat actcgatttc
     8221 atcacgtgtc atttgctcat ctgcatcatc aaaggtcatt ggggtaagtc ggctcagcaa
     8281 attagtagaa gcagataaga gccacacaaa aggactaacc agtctcccca acccaatgat
     8341 aataggggct gaaacgatcg ccaacttgtc tttgaggttc atggcaatcc gttttggata
     8401 caattctcct aaaacaatag agacataagt caagaaaacc aaggagatga tagtaccagc
     8461 tgtccttgca gtcgctgaat tacctagcca acctgagatc accttaccca aagaagctga
     8521 taaacttgct cctgatagta aactaataaa ggtaatccca acttgaatcg ttgataaaaa
     8581 atgattaggt tcctctaaaa cccgcaacaa acgagcgtat tttttatcac cgtctgctgc
     8641 tttttgttcc acccgagaac gattgagaga aactaaggcc atttcactgg ctgaaaaaaa
     8701 agcatttaac aaggttaaaa caactaacaa taaaaattga atcactaagg gctgactcac
     8761 agggtcttcc atgttaatgt aactcctaaa aaaataatat agtgacttca ttatatcaca
     8821 tttatagcca aagcctatgg gatttcttct ttttccaccg aaataatcgc agaacttcta
     8881 gtagacttga cacagggata agggctaaac gtctgctaac ttctccaaca tgattaaaaa
     8941 aggtggggta ttcacctgat taagaggctg ataaagcatt gcagaaacct ttctttggtc
     9001 taattgcttg acaaaatcta ataaagcatc tttttccaaa ctacctccat catgcccata
     9061 atagaccata atagcaatac gtccacctac cattaataac gttagtagtt ttgataaagc
     9121 ttggagtgtg gtatttggta aggtaataat agatttatct gcgctgggca aataaccaag
     9181 gttaaaaatc gctgcgcgca ccggctctgt cacatactga tcaactgctt catgacccgc
     9241 taaaatcagc tcagcgttca ataatcctag ctgcgctaac cgctcacttg tttttctaat
     9301 agcttgttct tgcacatcaa aagcataaac ctttttagct aattgtgcca aaaaggcagt
     9361 atcatttcca ttacccatcg tggcatcaac aaccacacta gacttatcca caacctccgc
     9421 taaaaaatca tgcgagagat gaatggggcg ttttagcatc acttttctcc tatccaaatc
     9481 cgtcaaaatg agacaaatta gtcaacttta cagccctgaa agcttcctcg tctttccatt
     9541 tctttatcaa tggcattcaa tacttcccac ttatttagac tccacatggg cccaatcaac
     9601 atatcgcgtg gggcatctcc tgtaatacgg tgaatcacaa tatgcttagg gataatctct
     9661 agctgatcac aaataatcga aacatagtcc ttttgactca acaactttaa gcgtccttca
     9721 tgataatctc tttgcattct agtattggtc atcaaatgca gcaaatgcaa tttaattcct
     9781 tgaatgtcat tgtctgttac acagcgtctc acattttcaa gcatcatgtc atgggtttct
     9841 tttggcaatc cattaatcaa gtgcgagaca atattaatgt tggggtaatg ccttagtcgc
     9901 ctcaccgtgt ctttatacaa atcataagaa tgtgctctgt taattagcct agaagtttct
     9961 tcataagttg tctgtaagcc tagctccact gtcacatgca tgcgttctga taattctgct
    10021 aagtaagcaa tggtatcatc tggtagacag tcaggccgtg tgccaatatt aatgccaaca
    10081 actccaggtt cgttaatagc ttgttcgtaa cggtcgcgaa tcacatcaac tgtatcatga
    10141 gtattggtaa agttttgaaa ataaaccaaa tagcgattga catcaggcca tttacggtgc
    10201 ataaaatcaa tttctttata aaattgctct ttaattggag catctggtgc tacaatagca
    10261 tctccagaac ctgatactgt gcaaaaagta catcctccat gagctaccgt tccatcacga
    10321 ttgggacaat caaagccggc atcaatcgga actttaaaca tttttgcgcc aaatagttga
    10381 cggtaatgct cgtttaatgt ctgatatctt tttttcatgt ttctattgta gcacaaatca
    10441 ttactagcaa gatagtcatc tatgcaaaag atagctcagt cattagaatt cactacttct
    10501 acatcagctt acttaatgat tacagatcaa caaaaatcac gtcttttctg acgttacata
    10561 gcactttaat catttttcat ctaaacatat ttactaatca tcagctcaca tcatctaact
    10621 aaaactaaaa taacactggc aatcaagggg tgatgcttgc caatgttatt ctaaatgatt
    10681 taaataagcc ctatgtcttc tcaagaaaaa ttagtcttgt ttcaaaagaa atcgccattc
    10741 aaatcgtttt tgcttataaa aaggataaat aaactgcaga atcccgaatc ctaatacaaa
    10801 gcctgctaaa atatcacttg ggtagtgtac ccctagataa atacgagata acccaattac
    10861 taaaattagt attataaaga tcatgctagt cacaaattgt aatagttttg aatgtagcct
    10921 ttgataacaa ataatcaata ggctaccaaa aatcagcatg ctacccatcg catggccact
    10981 gggaaacgaa tagccaccag cataaaccaa atgctcaata gcaggtcttg gtctttggta
    11041 aaagagcttg agagtcgtaa tcaaaaatgc tgcaatagca ccatttgata agataaacaa
    11101 ggcttcaatt ttccacttca taaagaataa aactagaacc gacacaataa ctaacaaaat
    11161 ttgtgtcatg acatttccaa atacagtaac acccctaaag aattgcgtca gcactatagg
    11221 aaggttgcct ctaatctctg cttgaatagt attatctaga agtgccaaac gttcaggaaa
    11281 aaatttgacc gtatagccta ttataacaaa gattagtaat gcaaaagaag ctattaaaaa
    11341 gtgagtttgt ttatttgtca tcatattagt gtaagtatct ttctaaaatt ggtttacttg
    11401 caatatacac aaaataaaag gtaattgcaa atatcaaccc ttcaacaaga ttaaacggaa
    11461 taaccatagt catcatatac ttagtgacac caatataagc tctgatatca atatttgcaa
    11521 atatagcata taaaggaatg gcaaaagtat aattgagaac aaccatgcca aatgttaata
    11581 accctgtgcc tagtaagcta gcaaatacat attgactaag cgttttttga cggttccaaa
    11641 ctaaggcaaa agccgtcaca aataaagcga tagcgataat attcattggg agaccaataa
    11701 aatcattaac cccacgattg ttcaaaaaga gtttcaataa ggacctcaat aacaaaatca
    11761 agtaagcact ctttaagtcc ataatcatta aaccaaatag gacaggaata atactaaatt
    11821 caatttttaa aaaagctgcg cctggaataa tggcaaagct aactagcatc aatagaaatg
    11881 atatcgcaga aagtatacca atcataatca ttttatgtgt ttttgacatt aaaaaattcc
    11941 tccaattttt tcaaattgaa agaagttccg tcgttattgt gtagctaaaa taagcctaca
    12001 caaaaagccc ggtcttctct catccagact atactgtcgg ttgtggagtt acaccacatc
    12061 agcttgcgct cgcggacttc tagttactag tcaccgccgg tcgggaatca caccctgccc
    12121 tgaagacact tctagcatag cagatttaaa cctagatagc aatagctttg actaaatttt
    12181 taaaagcgtc agctactgac aacgcatatt gccaaaaact gacgctttta aaaagtacat
    12241 tattttagtt tatcgtgttc gtaagtatgc tttaactcta acccttgaaa gccctgttgt
    12301 ctaagggctt cataaatcac catacaaacc gtatttgaga catttaaact ccgtacatgc
    12361 tcatcattca tcggaatacg aagtgctttt tcagcatgtt ctcgcataaa atcttctggc
    12421 aggcctgtat cttctcgtcc aaacaagaaa taatgggaat cgccatcagc ataagtagct
    12481 tgagaataat tattaactgc aaacttgcta atcaggtgaa gctgtccgtg acattgattg
    12541 ataaactgtt ctaaatgatc ataaaaatgc agttctaact tgtcccagta atccaagccg
    12601 gcacgcttca ttttgcggtc atcaatagga aatcccatgg gtttaataat gtgtaaaggt
    12661 gcattagttg cagcacatgt tctagcaata ttaccagtat tttgtggaat ttggggttga
    12721 aacaggacaa tatgattgcg agcctttttt actttgtcgt ttttgttgat aagttctttt
    12781 gtcgtcataa tctcatttac tctttttaat ttaaaaaacc acactgctcg gagtcatgct
    12841 cagcaaacaa tgtggttgat ggtgactcgc taacttaaat cataacaggt ttgatttaaa
    12901 ccaacgaatg cttgttttcc attataacac ttcagctatc aatgtcaagc cattttttga
    12961 taaagtcatg ccttttcagc gtttttagac gtgtttttat ttctcattag gctaaaatct
    13021 tctggaacag gatcgacacc accagcaaca aaagggtgac atcttaatat tcttgctatc
    13081 cccattagta ttcccttagt accatgtttt tcaatagcag ttaacatata agctgaacag
    13141 gttggcttgt agcgacaact aggaggagat aaaggagaaa tgtatttttg ataagcttta
    13201 acagatacta ttaatagttt tttcatcatt ttttcgtatt agctaagtta tgcagttgac
    13261 ttatttcttt tttatttaag cgtcttgctt ctcctggtct tagtcccttt aagtcgacgg
    13321 tcccaaaacg tgtacgagat aatttatcaa ccaaaagccc cacagactca aacatttttt
    13381 taacctgatg gtttcgccct tcatggatag tcaattctac gatcgatcga ttcttatcag
    13441 cttctacacg aacaatattg tagcgagctg gtttggtctt tttaccatca ataacaattc
    13501 ctcgagttaa gggacgaaga ttttctttcg ttgcaattcc tttgacacgc gccagataaa
    13561 ccttgtctat ttcatttcta ggatgaatca tagtatctgt aaaatcacca tcattcgtta
    13621 aaataaggac ccctgatgtg tcccaatcca atcgtccaac agggtatatg cgttctttaa
    13681 cctgaggtag taaatccagt accgttttac ggcctttgtc atctgacaca cttgagatag
    13741 cgccacgagg tttattaagc aaatagtaaa ccttttcctc gttgtaaata ggacttcctt
    13801 ctatttcaac cacgtctccc gattttacag tggttgctaa atctgtaata acctgaccat
    13861 ttaaggttac taagccttgc ttaatcaatt cttcagcctt acgacgactg gcaatacccg
    13921 catgggcaat gtatttgtta attcgcatta tattcttctt tcttttgagc aactttttgt
    13981 tgtccctagt ttaaggcttt tttcaactaa aacgccttaa aacaaatctt tttgtaaacg
    14041 tcatttgcta tttgatgaca agcaaaactc agtcttgtgt cctaaaaaga gcaatctctt
    14101 catctgccgg ttcaacggca gatacttcaa tcaattcgtc taaatgatta attcccatat
    14161 agtccagaaa ataatctgtt gtcgcataca aatgaggacg cccaaccaca tcttttttgc
    14221 cagcttctct aatgagatca aatgccaata atttactcaa agctccactg gagtttaccc
    14281 ctcgaatgtc atcaatttct attcgtgtaa taggttgttt gtatgcaaca atagacaaga
    14341 cctctaaact cgcacgagat agactttggt tcattggtgt tttggcataa gcacgcaaga
    14401 gttctgcaaa accttcttta gttactagcc tgtaggtatt agctgtttct atcaaacaaa
    14461 gactagaatg ttgatctttt tcatactttt gagagagttt ttctaattgc tgttgaagag
    14521 ctgttggcgt taatgataac atagacgcta gatgtcgcaa acttaaccct tcttcacctg
    14581 caacaaatag cagcgcttct atctgtgaca aatacgtcat ttcttctcct ttcgtagtat
    14641 gatagcccca aaattagaga gctgctctac aaataccacg tgaagcttaa tcaattctag
    14701 actagctaaa aatatactga taacttcatt taatgtttgg cattcctcaa aaatcgctga
    14761 taagctaata acgttttctt tttccagtcg ctgtttaata gaagccatca tgtcttcaat
    14821 cttataatca tcacgctcga tgactgtatg gttgtttttt aaaactgctc ttttagctgc
    14881 cattatgttt gaaaaagcta gaaatagatc catcaccgtt ttatcttctt gtaagatagc
    14941 atcttcaaaa attaattctt gtttaggttt tgaataccat tttgcccttt ggtcatgctg
    15001 cttcgctaac gcttgactaa ctgctttaaa gcgactatat tcttcgatct tttctaacag
    15061 atcttgttct aaatcttctt cttcgatgtg ttctaccacc tttggtaata gccttctact
    15121 tttgattaac atgagctgac ttgccatcag catgtagtca ccagccactt caagtttcat
    15181 tacctgtaag gtttcaatat aattaaggta ctgctcaata acctcaacaa taggaacctc
    15241 gtagatgtca actttgtact gtgataccaa gtgcaagagt aaatcaagag gaccttcaaa
    15301 atctttaagt tttatatcca ttatgatttg tagtattttt ctaaggtcac agggcttttc
    15361 agcccaagaa tatcgctaag ttctattatc gatttaccag ctagtttttc tttcaaaata
    15421 aattgctctc gtaatttttg agcagttaat ccttcataac caatactccg aacaaaggtt
    15481 tttaggtgat taaaaaacca ttgtcttgaa aaagggatgc ctcgatgttc aaaaagatag
    15541 acttctttgc caatgagttg ttgttctaaa aaagggatta gtatttgcga caaaggtaat
    15601 acacgcacac cttttttagt ctttagcgtc aacatttgaa aatttaaatc caagttagct
    15661 acttcaatac cagctacttc actcggagtt aaacccagct ccaatattaa taagctgatt
    15721 aattgccccc acacaaaagg ggttttttga tagaaaatat cagtattaat aatagcagct
    15781 tgctgcttct cagtccgcat cactttcatg gtatctgtta atcgaaaata gctatttaaa
    15841 tactttattt gatacaagta atataagaat tgattagctg ttgataattt gcgttttttt
    15901 gcagataaac ttaggttagc tatgctattt tgataaagta atagtttgtc ttgattgaca
    15961 cgttcaccaa ccagttgaca aaactgttgc aaatcgtatc gataagcttt ttgactattt
    16021 tgagataaag ccttgctagc aataaaaggt tcaatataac tcttcatttt tttataatcg
    16081 tgtaatcatc tgtaaaatca tgtaaaagac tgttgacagc ctttaatata gattttcgcg
    16141 taataatccc aacaaagcga ttcgctctat ctacaaccgg taagaatggg taatcaatta
    16201 acttgtgcat aatttctgtt aggctagaag taatgctaat ggtttcaatt ttggtattaa
    16261 ccatctcgcc aatatctgtt tgactcattt cccaatctgt cagctgcctt ttagattggt
    16321 acatcataat atctgagatg ctaatggttc ccacatattt tttctcccgt gtgataacag
    16381 gaactctaga aaaaccatta ctcaccaaaa gtaacatcac atggtctgca ttatgtgtgt
    16441 cgataaaaat agccaagtct tgttccggga taagataatt atctaaatga ctcattaaaa
    16501 acgtttcaaa ttcttttgcg atcatcgttt aaattcctta gacagtgatg ggtaaagttt
    16561 atgatctctt gtaaagtaat caactttaat ttgagtatca gttagttcaa cacgagcata
    16621 taatttctcg ttaatttctc cgcgaggttg tgttactgat ccgggattca taaagagggt
    16681 ttgcccaact tgccaagcag ctggacggtg taggtgtcca tatagacaaa tatctgctgc
    16741 tacttcttga gcaaagtagt ccagtttatc ccaagtgaaa ttaatatggt acaagtgacc
    16801 atgtgtctgg gcaatcgtaa ctgtgcctaa ttgagttact aaacgatcag ggtaacctgt
    16861 atcatagtca caattaccgc caacgacata aataccagcc caaataggat ctgagctgtt
    16921 taattcagaa tccccattat ggaaaatagc atcaacttga ccaagatact tatctttaat
    16981 ggcttgaacg atatctcggt caccatgaga gtcactcatt actatgattg ttttgcttgc
    17041 catgctggaa agacctccat taattttcga actgcttgac ctctatgcga caactgattt
    17101 ttttggtcag cctctaattc tgcagcatga cgcccagtct ctccgacgat aaagacagga
    17161 tcatacccaa aaccattttc tcccttaggc tgcgtagcaa tatagcctgg ccactcggct
    17221 tccacaacca aactatcttt attgggagca gccactacta aagtcgtatg aaattgagct
    17281 gagcgcttct tttggtcaaa caccatagct aattcgtgta agagtttggc attattcttg
    17341 gcatctgtgg catctggtcc tgagaaacga gctgaccaaa caccaggtaa accacccaag
    17401 gcatcaactt ttagtcctga atcgtctgct aataccattt ttcctgtgag gcgtgaaatc
    17461 gtttctgctt tcaagcgagc attttcttca aaagtagtac cagtttctgc tacttctggc
    17521 aattctggat aatcattgag attttccaca cgataaccta gctggccaaa cagattgcgg
    17581 aactctttgg tttttccctc gtttcgtgta gcgattaaaa taatatcccc aaatccctgt
    17641 tcactagtcg caaaaaatgt agccgttgaa actccacctt ctgggagata aactgcaagc
    17701 agtttcgcat tttccattaa taaaatcgtg cctgcatggg gagttacctt aatcttacgc
    17761 tgggtctctt gatttatcat gtcgataata aaggtgatga gagataaagg agactgataa
    17821 cgcaccaccg aaacatcgta acctttagga ttttcccaat ctaacgttgc ggcaataccg
    17881 tctaaaaggt catctatttt tttgattgtc gtgtgtttga ctccccagcc cgatattaaa
    17941 ttatgaccag tcatctttcc gataaaccag ttattttcat ctttgtattc gtaaattttt
    18001 tcactcataa ggtgacatgc tccacattta ttttttgttt cagccaaatc gaggcaattt
    18061 cttgaaaaat ctcagaattt gctgttgtaa aaaagcggtg ttcgacagct ttttgatgat
    18121 agttaccgtt aatgtcaaaa tagtttaata agacagagat atctcggacg cattctgctc
    18181 cactgtcaat cagcttaaca gatggcccca taacattttg gataattggt cgtaacaagg
    18241 gatagtgagt acatcctagt acaagggtat ctattttacc gactaatggt gctagactgt
    18301 cataaactat ttttttagct atactcgaac acatctcatt tgattccaca atcggtacaa
    18361 acttcgggca agcaagactc cttacttgaa cagatggtgc taatagctgg atttttttgc
    18421 gataaatgtc tgaagccact gtcattgggg ttccgatgac cccaacctgg ccttttgtcg
    18481 ttgatttaat agctgcgctt gcccccggta agacaacccc taaaacagga atatctaaag
    18541 ctgcttttac ttcttcccaa gccaccgctg ttgcggtatt gcatgcaaaa acaatcattt
    18601 taacattttg agtcagcaaa aaattgacta gctcccaagt atactcctta atttgttttt
    18661 tgggtctagg accatagggg gctcttgctg aatcaccaat atatacaatc ttctcatgag
    18721 ggagctgacg aatcaattca caaacgacgg tcagacctcc cacaccagaa tctaaaaacc
    18781 caattggtct tgtatccatt atctttactt ccttactgac gatatgaaaa actcagttgg
    18841 aaatagccaa ctgagtttct ttttaaaccc tgaacagttg acaaatctaa ctgataagac
    18901 aagtgctact gaacattgag atcaaagact gttcacttct aactatcaca aaggtttccc
    18961 ctataacaat tagcttttat tttttacctt tggatacggc tgcttttgat tgtttgatga
    19021 tattacgata agtttgttgg atttttgctt cacttggttt ttgccccatc tggctcatca
    19081 tttcacggat agcttctggt gttaaacgtg ggtgttcacc aatttctttt tcaatttgtt
    19141 tacgggcaat aaaaatccca ccaaaaacac ctaccccaag ggcaacaata agtaataaaa
    19201 tccaaatagc tgttgacata tctatctata ttctcctcgt
//
LOCUS       DAUELZ010000028        19214 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.028, whole genome shotgun sequence.
ACCESSION   DAUELZ010000028 DAUELZ010000000
VERSION     DAUELZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19214
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.028"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            582..752
                     /locus_tag="VHI09_000741"
     CDS             582..752
                     /locus_tag="VHI09_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120633.1"
                     /translation="MDNPKKDKGLSLGVVDPITILITESVINKMRTVPYHLKKLSSTT
                     YCLLADLAYQSA"
     gene            1024..2220
                     /gene="speB"
                     /locus_tag="VHI09_000742"
     CDS             1024..2220
                     /gene="speB"
                     /locus_tag="VHI09_000742"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEQ6120634.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSAGSSRVQRALKENFGYNQSVHQINRSDFSKQDWEAQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            2222..2527
                     /locus_tag="VHI09_000743"
     CDS             2222..2527
                     /locus_tag="VHI09_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEQ6120635.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMVEAYQKQINSLD"
     gene            3007..3936
                     /gene="prsA"
                     /locus_tag="VHI09_000744"
     CDS             3007..3936
                     /gene="prsA"
                     /locus_tag="VHI09_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ6120636.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTIAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPAISKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(4207..4443)
                     /locus_tag="VHI09_000745"
     CDS             complement(4207..4443)
                     /locus_tag="VHI09_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120637.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPAAHSPCD
                     DTHQLLEDKSLSDDYLIATSAMRHLWYWGYSQIG"
     gene            4868..7861
                     /locus_tag="VHI09_000746"
     CDS             4868..7861
                     /locus_tag="VHI09_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ6120638.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSTSASSTKTSAS
                     TNINTNTSTASAGTGTSGTASTTPSVGTSTGGAAGGEAAVASSGGSQSSESAQASTQP
                     QAQTAVAASASTTASPSSSEEKTPKTVTSSTSSTPAASSSSNGNQVTGTEVEPQMMDV
                     EQYKVNKEKTELTVKDDKQQLKIRKDVDELKNKDLFDVKREVKDNGDGTLDVTLKVMP
                     KQIDEGADVMALLDVSQKMTQENFNKAKEQIKRLVTTLTGKSSDGKENHNRRNSVRLM
                     TFYRKISEPIDLSGKTSDEVEKELNKIWDKVKKEDWDWGVDLQGAIHKAREIFRSSYE
                     KSRANANIKSGKRQHIVLFSQGESTFSYDIKNKNDNKLTKARIEEEVTSSNPLLSWPP
                     IFNHTNRKADMLNDIEYLIKLGERLGITGLDSLKNTLKLASTGSSIAGSLLGSGSLSE
                     YLTLKEYESRTLKESNFDYTKRVGEGYYYHSFSERIQNELPLKSIIEPQLKGLFKTED
                     SSWFGRFLNKFSLAKGYQEFKETALLKVLEYLFYKREYIYYNHNLSAIAEAKMAQQEG
                     ITFYSVDVTSPNQSANKRTRRSADTPEEKRNKKFDNYLKEMSEGRKFLEGQDVTNKDK
                     FKDTLTELTIKDEFTEKVTVHKDKEYYKTSLIDDRPKVTHQAPYSSWLNSTKESLTWT
                     ISKDQLKKAFESGQPLTLTYKLKVEKEKFKEALKKQQERKKRAASPESENTVTDTIIS
                     NKISYKINNGTDINSNNNKLEDVKMSYSKFKMPIPELDIEVVPIPEKPLVEPMTPLYP
                     AIPNYPTPDIPTPQLPKDEDLEISGGHGPIVDIVEDTGTGVEGGAQNGVVSTQENKDP
                     IVDITEDTQPGMSGSNDATVVEEDTTPKRPDVLVGGQSDPIDITEDTQPSVSGSNDAT
                     VIEEDTKPKRFFHFDNEPQAPEKPKEQPSLQDSNSLPQAPAYKAAHHLPASGDKREVY
                     FTIAALTIIGAAGLLSKKRRDTEEN"
     gene            8041..9972
                     /locus_tag="VHI09_000747"
     CDS             8041..9972
                     /locus_tag="VHI09_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ6120639.1"
                     /translation="MTKKFIKSAVILGLASITISMTQPVEAIANRLEYQEQYERVKKE
                     LDALKEKGPKLGERSLYFRVVNKFLSWFGQVWDPLAIEETDHLLDLIGQVNYLVSNTE
                     FQAPEYQEAKNKLDLAVAGYDLYLSREKGRGDAPDYQVTQLTLEQIATIKTKIQEALK
                     TFAQEVKTIQQTHPDLFPHAYVLNAKTSAIDGKAQPFLVNGYYDVFYKQFLFTNTKDG
                     YNYNKEADNLTSYQNLIKELLELKTKIDQLTTDKPKNDLLETLMWQSKFTLSDFRRTS
                     RLSAYYTQEDYVKKVVLSEEAPNLRTAKEKVKTLKGILHDYYVETNDLEKAKEYHVED
                     ETSPSQPEAPAKPEAPSPSPAPGQKPAEDERSSQATEPANPSKEDSSTDASQGSHDSE
                     NPATDSPSQPQAPDQGNHQSQVPNDKPQTDKTDTPNVPAPPQDTPKVPEAGGQSGPAG
                     NAEEKAPDSGPKESDQSSSKESPSVGEDTTPNQPDVLVGGQSEPIDITEDTITDAPPT
                     VSGHNASTQPQSVVEDTAPQRPDVLVGGQSEPIDITQDTQPGMSGSNDATVINEDTKP
                     KRVFHFDNKESQASEKAAEQKLAPHDSHTTPQASDDTAAPHLPASGDKPEKVFALAAL
                     TMVAAAGLLSKKRRNTDKN"
     gene            10114..10410
                     /locus_tag="VHI09_000748"
     CDS             10114..10410
                     /locus_tag="VHI09_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEQ6120640.1"
                     /translation="MTKQDQLIVEKMEQTYETFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADILDLAPIMYKHM"
     gene            10558..10842
                     /locus_tag="VHI09_000749"
     CDS             10558..10842
                     /locus_tag="VHI09_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEQ6120641.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            11266..12534
                     /locus_tag="VHI09_000750"
     CDS             11266..12534
                     /locus_tag="VHI09_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ6120642.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATTEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            12521..13195
                     /locus_tag="VHI09_000751"
     CDS             12521..13195
                     /locus_tag="VHI09_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120643.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            13208..14425
                     /locus_tag="VHI09_000752"
     CDS             13208..14425
                     /locus_tag="VHI09_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6120644.1"
                     /translation="MEDIIAALGSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            14519..15172
                     /locus_tag="VHI09_000753"
     CDS             14519..15172
                     /locus_tag="VHI09_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ6120645.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            15169..16557
                     /locus_tag="VHI09_000754"
     CDS             15169..16557
                     /locus_tag="VHI09_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ6120646.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLM
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKPGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            16659..18287
                     /locus_tag="VHI09_000755"
     CDS             16659..18287
                     /locus_tag="VHI09_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ6120647.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNTSSTDQPTTAD
                     NDTDDESETPQKDKKSKETASQHDTQKDHKSSHTHPTPPSNDTKQTDQASSEATDKPN
                     KDKNNTKQPNSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKSADKA
                     PEKGPEKAVEKTPEPNRDTPKPIQPPLAAAAPVFAPWRESDKDLSKLKPSSRSSAAYV
                     RHWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVR
                     AIVAIAMAESSLGTQGVAKEKGANMFGYGAFDFNPNNAKKYSDEVAIRHMVEDTIIAN
                     KNQTFERQDLKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMTKLDQWIDDHGSTP
                     EIPEHLKITSGTQFSEVPIGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVD
                     RYMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILIS
                     ESNVMGLGTISYRTFTAEQASLLTYVVGDKLPRP"
     gene            18365..18616
                     /locus_tag="VHI09_000756"
     CDS             18365..18616
                     /locus_tag="VHI09_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120648.1"
                     /translation="MIKKVTTPSQKTKKRVRNGYLLKLGTACLLLSILSYGIGLLGQP
                     SMENTFVGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
BASE COUNT         6797 a         3914 c         3766 g         4737 t
ORIGIN      
        1 ctagtgagat aatcaccaca aacttgttta atcgaaatgt tttttgaatg cctaatgaat
       61 tcaacggttt caccaatttc catatgtcaa gccttcctag ttgatgtcaa aaatacgtta
      121 cgcatgtacc taatacgtaa caagttgaat gtttcggatg atagtcgctt atgataggtg
      181 cataaggtca atagccagat gcgatagttt tatcaactgt catatgttaa acctttctaa
      241 tctaatagat gttaaaaata cgttacgtgt gtacctaata cgtaacaaaa taatgggtta
      301 gcaaaataag cagctatgat atagccataa ggttaaaagg aggcgcctac tatgtggtta
      361 ttgttactat ttttgtagtt ccttttgcaa aatggtagta gataaaaatg agaagtcaat
      421 aaaagcattg acaaacaaaa gaatcatttt gtttatatta tcagtatttt taagtttctt
      481 gtcaactgaa atgagcatct actagccaca atagtaacta aaaggctaat tgtcagtaaa
      541 aatgagataa atcagccagg aaaaagccta cgagtcaact aatggacaac ccaaaaaaag
      601 acaaaggact ttcacttggt gttgtggacc ctatcacgat ccttatcaca gaatcagtta
      661 ttaacaaaat gagaacggtc ccatatcatc taaaaaaact gtcaagcaca acctattgtt
      721 tgctagctga tcttgcctat cagtctgcct agtagatacg gcagaagata agagatcaac
      781 caaaagatga taagtgcaac gactcatagc gtcttttgac caaaaccaat taggctcaca
      841 tgagatgaga ataattgggt tgggttgtca gtgtcaacta accgtgttat tgtctattac
      901 cattcatggt atcagcgaca tcgtatgata accatacgat tcagctaagt aaggaggtgt
      961 gtccaatgta ccgttaaaag caaatgcagt agattaactt attttgaaag aggtataaaa
     1021 aaaatgaata aaaagaaatt aggtatcaga ttattaagtc ttttagcatt aggtggattt
     1081 gttcttgcta acccagtatt tgccgatcaa aactttgctc gtaacgaaaa agaagcaaaa
     1141 gatagcgcta tcacatttat ccaaaaatca gcagctatca aagcaggtgc acgaagcgca
     1201 gaagatatta agcttgacaa agttaactta ggtggagaac tttctggctc taatatgtat
     1261 gtttacaata tttctactgg aggatttgtt atcgtttcag gagataaacg ttctccagaa
     1321 attctaggat actctaccag cggatcattt gacgctaacg gtaaagaaaa cattgcttcc
     1381 ttcatggaaa gttatgtcga acaaatcaaa gaaaacaaaa aattagacac tacttatgct
     1441 ggtaccgctg agattaaaca accagttgtt aaatctctcc ttgattcaaa aggcattcat
     1501 tacaatcaag gtaaccctta caacctattg acacctgtta ttgaaaaagt aaaaccaggt
     1561 gaacaatctt ttgtaggtca acatgcagct acaggatgtg ttgctactgc aactgctcaa
     1621 attatgaaat atcataatta ccctaacaaa gggttgaaag actacactta cacactaagc
     1681 tcaaataacc catatttcaa ccatcctaag aacttgtttg cagctatctc tactagacaa
     1741 tacaactgga acaacatcct acctacttat agcggaagag aatctaacgt tcaaaaaatg
     1801 gcgatttcag aattgatggc tgatgttggt atttcagtag acatggatta tggtccatct
     1861 agtggttctg caggtagctc tcgtgttcaa agagccttga aagaaaactt tggctacaac
     1921 caatctgttc accaaattaa ccgtagcgac tttagcaaac aagattggga agcacaaatt
     1981 gacaaagaat tatctcaaaa ccaaccagta tactaccaag gtgtcggtaa agtaggcgga
     2041 catgcctttg ttatcgatgg tgctgacgga cgtaacttct accatgttaa ctggggttgg
     2101 ggtggagtct ctgacggctt cttccgtctt gacgcactaa acccttcagc tcttggtact
     2161 ggtggcggcg caggcggctt caacggttac caaagtgctg ttgtaggcat caaaccttag
     2221 tatggaaatg catttcgtta gaacagaacc tgaggcacgc cgcatagctg aaaccttttg
     2281 tgccgaaaac acacaaacaa aaacccctat gcgcgtgcag cagttaagct atccatcaga
     2341 cacagatcac tcaggtggtg agctatacat ctatgctttg tctcctgctg gatttatcat
     2401 cgtatcagga gataccagag cgcacaccat tttaggctat tcttttgata ataacctgga
     2461 cctcaaccat gataatgtca gaagtatggt agaagcttac caaaaacaaa tcaactcttt
     2521 ggattgatcg gtcaaagtag cttttgcccc ctcaccactc gtttaagacg accgtgtttc
     2581 taaaatacca ctacctttta agtttcacgc cggcctaagc aagtgtttgt ctcatggagc
     2641 agactgtttc catcgaaaag tcacccattg ataaaggagg tcatcaacgc ctactaatga
     2701 tgacttccct aagatgccaa ggtaaaggac tgagttctgt agagatggga ctcagtcctt
     2761 ttacgatttt tgggagttat tctagccata ccgtggcaag aaaacaacac tcaaaaatag
     2821 ccaacgtcag tttctgttca tacatgacta cagccaaaca ccacaaccta acctatccta
     2881 aacacagtgg ctttctaacg aacagtcaca acgagcagca acaccaaaaa gcaagattaa
     2941 gctacgttta agactaatga tataagataa cagtaattat taatgagaaa agaggctcaa
     3001 aaggacatga aacaaatgaa taaactcatt acaggagtgg taacgctggc gacagtcgtg
     3061 accttatcag cttgtcaatc atcacacaac aacaccaaac tcgtctcgat gaaaggagac
     3121 accatcactg tcagtgactt ctacaatgag accaaaaaca cagaactcgc acaaaaagcc
     3181 atgttaagct tggtgattag ccgcgttttt gagacacaat atgccaacaa agtctctgac
     3241 aaagaggttg aaaaagccta taaacaaacc gcagaccaat acggtacatc ctttaagaca
     3301 gtcctagcac aatcaggctt aacgccagaa acctataaaa aacaaattcg cctcacaaaa
     3361 ttagtcgaat atgccgtcaa agaacaagcc aaaaacgaaa ccatctcaaa aaaagactac
     3421 cgtcaggcct atgacgctta taccccaacc atgaccgcag aaatcatgca gtttgaaaaa
     3481 gaagaggatg ccaaagcagc gcttgaagcc gtcaaagctg aaggggcaga ctttgcagct
     3541 attgccaaag aaaaaaccat tgcagccgat aaaaaaacaa cctatacgtt tgactcaggc
     3601 gaaacaaccc taccagcaga agtagttaga gctgcatcag gcctcaaaga agggaacaga
     3661 tcagaaatca tcacagcgct tgatccagcc atctcaaaac gcacctacca tatcatcaaa
     3721 gtcaccaaaa aagcaactaa aaaagcagac tggaaagcgt accaaaaacg cttgaaagac
     3781 atcattgtga ctggcaaatt aaaagaccct gacttccaaa acaaagtcat cgctaaagct
     3841 cttgataaag caaatgtcaa aatcaaagac aaagcatttg ccaatatctt agcccagttt
     3901 gcaaaaccta accaaaaaca acctgcccaa aaatagaggg ttgccagttt atttccctaa
     3961 ggttgcttaa aaagacagcc ctagccatca ctcccctttt caaaacaccg ataatggaaa
     4021 cgtgtcctat actgatggtc tagggcaatg tcataaagct aaagactcta tccaaataag
     4081 gatagagtct ttaatattta aaaaccagtc agcaaaaaaa ttcacgctaa gcctagaatg
     4141 aggcttagcg tgaatggtat cagtgatagt ccgttaacat agctgattgg gagtcagaaa
     4201 ctctaactac ccaatctgac tataccccca gtaccagagg tgtctcatcg cagaagtagc
     4261 aatcagataa tcgtctgata agctcttgtc ctcaagcaac tgatgagtgt cgtcgcacgg
     4321 gctatgagct gccggtggct gatagctagt aggctcatca gccaaaactt ttttggagta
     4381 acgctttggt ctcagacggc gccagagggt tgagagaggg gtgagaaaaa aacgatgggt
     4441 catcttggcc tccttgtcta atgatagtca cgttaacaga caaaagtgtc aataatgtca
     4501 ttttaaggag aaaaatatta aaaatcaagg tcacaaactt tcctttgtta aaaaaactcg
     4561 accctaacaa agaacctaaa tacccctcgc acttgagctt ctccttgcgc agctatcttt
     4621 tgtactcagt tgcatacaag ctaatgatag cgataccatt attgtcctat aggctatcgc
     4681 gacaacccct ttttttcgcc tcaatgaggc tgaaacagct gtcatgacag agaaaagacc
     4741 cttgtctatc taagagacca ttaattttta tctcaccaaa aaactgattt tagaaacaaa
     4801 aaatcatggt gtataataaa gttcggaaca attatgacat tataatgaaa gtaaggttaa
     4861 cgaaacattg acaaattgta agtataaact tagaaagtta tctgtagggc tcgtctccgt
     4921 cggaacgatg ctgatagccc cgacagtttt aggacaggag gttagtacta gtgctagtag
     4981 tactaagacg agtgctagta ctaatattaa tactaatact agtaccgcta gcgctggtac
     5041 cggtacgagt gggacggctt ccactactcc tagtgttggt acaagtactg gtggagcagc
     5101 tgggggtgaa gcagctgtag catctagtgg aggaagtcag agttcagaat ctgcccaagc
     5161 ctcaacacaa cctcaagcac agacagctgt agcagcatct gcttccacta ctgcgtcccc
     5221 ttctagtagt gaagagaaaa ctcctaagac agtaacttca tctacctcat cgactccagc
     5281 agccagtagc agtagtaatg gcaaccaagt aactggtact gaagttgaac cacagatgat
     5341 ggacgtggaa cagtataagg ttaataagga aaaaacagag cttactgtta aagatgacaa
     5401 acagcagctc aagatccgta aagatgttga tgaattaaaa aataaagatc tttttgatgt
     5461 caaacgtgaa gtaaaagata atggcgacgg aaccttagat gtaaccttaa aagtaatgcc
     5521 taaacaaatt gacgaaggtg ccgatgttat ggccctttta gatgtctctc aaaagatgac
     5581 acaggagaac tttaataagg ctaaagaaca gattaaaaga ttagtcacaa ctttaacagg
     5641 caaatcatct gatggaaagg aaaatcataa taggcgtaat tctgtacgtt taatgacctt
     5701 ctaccgtaaa atcagtgaac caattgattt atcaggaaaa actagcgacg aagtcgaaaa
     5761 agagttaaat aagatatggg ataaagtcaa aaaagaagat tgggattggg gcgttgattt
     5821 acagggtgct atccataagg ctcgagaaat ttttagatct tcttacgaaa aaagtcgggc
     5881 aaacgccaac ataaagtcgg gcaaacgcca acatatcgtc ctgttctctc aaggcgagtc
     5941 aacctttagt tatgatatta aaaataaaaa tgataataaa ctcacaaaag cgagaataga
     6001 agaggaggtc acgtcctcta atcctctttt atcctggcca ccaattttta atcatacgaa
     6061 tcgaaaagca gatatgctta atgatataga gtatcttata aaactaggtg aaagattggg
     6121 aattacgggt ctagatagtt taaagaatac actgaagtta gcgtcgacag gaagttctat
     6181 agcaggctca cttttaggca gtggtagtct gtcagagtac cttaccttga aggagtatga
     6241 gtctagaaca ttgaaggaaa gtaattttga ttatactaaa cgtgtgggcg aagggtatta
     6301 ttatcatagc ttttctgaaa ggatacaaaa cgagttacca cttaagtcaa ttatagagcc
     6361 tcaactaaaa ggattattta aaacagagga tagtagttgg ttcggtaggt ttctcaataa
     6421 gttttcatta gcgaagggtt atcaagaatt taaagaaact gcccttttaa aagttctcga
     6481 atatttgttc tataaacgtg aatatattta ctacaatcac aatctctcag caatagctga
     6541 agccaaaatg gctcaacaag aaggcatcac cttctactcc gttgatgtca cgtctccaaa
     6601 tcagtcggct aataaaagga ctaggaggtc agctgataca ccggaggaaa aacgtaacaa
     6661 aaagtttgat aattacctaa aagagatgtc cgaaggaaga aaattcttag aaggtcagga
     6721 tgtcacaaat aaagataaat ttaaagatac tttaacagaa ctaacgatta aagatgaatt
     6781 cacagaaaag gttacagttc ataaagataa agaatattat aaaactagtt taatagatga
     6841 cagacctaaa gtaacacatc aagcccctta tagcagttgg ttaaacagta ccaaagaaag
     6901 cctcacttgg accatttcca aagaccagtt gaaaaaagct tttgaaagtg gtcaaccatt
     6961 aaccttaact tataagctaa aagtcgagaa ggaaaaattc aaagaagcac taaaaaaaca
     7021 acaagaacga aaaaaacgag cagcatctcc ggaaagtgag aatactgtca cagacacaat
     7081 tatttcaaat aagatttctt acaagattaa taatggtacg gatataaata gcaataacaa
     7141 taagttggaa gatgttaaaa tgtcttacag caagttcaag atgcctatac cagaacttga
     7201 tatagaagtt gtaccaatac cagaaaaacc actggtagaa ccaatgacgc ctctatatcc
     7261 tgcaattcct aattacccaa caccagatat ccctacccct caacttccaa aagatgaaga
     7321 cctggagatt agtggaggtc atggaccgat tgtcgatatc gtcgaagata ctggtacagg
     7381 tgttgagggc ggcgctcaaa acggcgtggt ttcaactcag gaaaataaag atccaatcgt
     7441 tgacatcacc gaagataccc aaccaggtat gtcaggctct aatgacgcaa cagttgtcga
     7501 ggaagacaca acacctaaac gtccagatgt tcttgttggt ggtcaaagtg atccaatcga
     7561 catcaccgaa gacacccaac caagtgtatc aggctctaat gacgctactg ttatcgaaga
     7621 agatacgaaa ccaaaacgct tcttccactt tgataacgag ccacaagcac cagaaaaacc
     7681 taaagagcaa ccatctctac aagatagtaa cagcttacca caagctccag cctataaggc
     7741 agctcatcac ttgcctgcat ctggagacaa acgtgaagta tactttacaa ttgctgctct
     7801 aacaattatt ggagctgcag gtttgctcag caaaaaacgt cgcgacaccg aagaaaacta
     7861 accctcgtta gtctagcgac cctaagcctt tggctttaaa aatttctcat tatccccctc
     7921 aaacaccggt acctcactga gatggcgctt aaaaaagccg ctagtctcaa atcaggtatc
     7981 ccctacggtt gagggggatt aatgggtatc acaacaagaa aaaaggtata atgatgaaat
     8041 atgactaaaa aatttattaa atcagcagtg attctaggct tagcaagcat aaccatcagc
     8101 atgacgcaac ccgttgaagc cattgcaaat cgcttagaat atcaagaaca atacgaacgt
     8161 gtcaaaaaag aactagacgc attaaaagaa aagggcccaa aattaggaga acgttccctt
     8221 tattttagag tggtgaataa atttctaagt tggtttggcc aagtctggga cccactcgca
     8281 atcgaagaaa ccgatcacct gttggacctc atcggacaag tcaattactt ggtgagtaac
     8341 accgagtttc aagcccctga gtaccaagag gctaaaaaca aactagattt agctgtagca
     8401 ggctatgacc tctacctatc tagagaaaaa ggacgtggtg atgctcctga ttaccaagtg
     8461 acccagctaa cgctagaaca gatcgcaacc atcaaaacga agatacaaga ggccctgaaa
     8521 acatttgcac aagaggtcaa aacaatccaa caaacacacc cagacctatt tccccatgct
     8581 tatgttttaa acgctaagac cagtgctatc gatggtaaag cgcaaccctt tttagtgaat
     8641 ggttactacg atgtctttta taagcagttt ttattcacta atacaaagga cgggtataac
     8701 tataataaag aagcagacaa tctgacctcg tatcaaaatc tgattaaaga actattagag
     8761 ttaaaaacta agatcgatca gttaacaaca gacaagccta aaaacgatct tttagagacg
     8821 ttaatgtggc aatcaaagtt caccttatct gactttcgga gaacttcacg ccttagcgcc
     8881 tactatacac aagaagatta tgtcaaaaag gtggtcctat ctgaagaagc tcccaacttg
     8941 agaacagcaa aagaaaaggt caagacctta aaagggatct tgcatgatta ctatgttgaa
     9001 actaatgatt tagaaaaagc taaagagtat catgtcgagg acgaaacctc accgtctcaa
     9061 cctgaggccc ctgctaaacc tgaagctcct agtcctagtc ctgctcctgg tcaaaaacca
     9121 gcagaagatg agagatcatc acaagctacg gaaccagcta acccgtcaaa agaggattcc
     9181 tcaacagacg catctcaggg atctcacgat agtgaaaacc cagcgacaga cagtccttct
     9241 caacctcagg caccagacca aggcaatcac caaagccaag tgcctaacga caagcctcaa
     9301 acggacaaga cggacactcc taacgtccca gcccctccac aggacacccc aaaagtgcct
     9361 gaggctggtg gtcagagtgg ccccgctggg aacgctgagg agaaagctcc tgactctgga
     9421 cctaaagagt ctgatcaaag ctcatctaaa gagtccccat ccgttggcga agacacgacc
     9481 cctaatcagc cagacgtcct tgtaggtggt caaagtgagc caattgatat caccgaagac
     9541 acaatcactg acgctcctcc gacagtatct gggcacaacg catcaacaca gccccaatct
     9601 gtggtcgaag acacagcacc tcagcgccca gatgtccttg taggtggtca aagcgagcca
     9661 atcgatatca cccaagatac ccaaccgggc atgtcaggct ctaatgacgc taccgttatc
     9721 aatgaagata caaaaccaaa acgcgtcttc cactttgata acaaagagtc acaagcatca
     9781 gaaaaagcgg cagagcaaaa acttgctcct catgatagcc acacaacccc tcaagcttca
     9841 gatgatacag cagcccctca cttgcctgca tctggagaca agcctgaaaa agtgtttgca
     9901 cttgctgcct tgacaatggt cgcagctgca gggctactca gcaaaaaacg tcgcaacacc
     9961 gacaaaaact agtcgctgtt aggcacaacg catcactaag gccagtgcaa atgcactggc
    10021 cttagttagg tttttatcaa atagagggtt tttcatgaca attgctagca tttcggtgta
    10081 aaataaaggt aatagccagc aaaggagcaa cccatgacaa agcaagatca gttaatcgtt
    10141 gaaaaaatgg aacaaaccta tgagaccttt agcccaaagc tagcaaatct catagaagcc
    10201 ctagatgcct ttaaagagca ttatgaggaa tatgccacac ttagaaattt ttacagtagt
    10261 gacgaatggt ttcggcttgc aaaccaacct tgggatgata tcccgtgcgg tgtcctatct
    10321 gaagacctcc tctttgatat gatcggtgac cataaccaac tactagctga tatcttagac
    10381 ttggccccta tcatgtacaa gcatatgtaa ccatcgctaa cgtcacagat actacgatga
    10441 caggacaaaa aaagcttgaa aatctcaaaa aaaacagata aaaaacagat aatcgttaga
    10501 aaaacagtag atgcgtctgt taaactaaag gtatcaaaac aaaaggagca tttcatcatg
    10561 aaattcaaaa aagtattagt cattcctgca ttagcattag cagcaacatg ctttttaaca
    10621 gcttgtggca caaaaaaaga ttctaaaaaa gaagaagtta aagaaatcaa aatgtctgac
    10681 atcaaagacg acgcagttag caaaaaaaca aaagtggttg atggcgaaga agttacagaa
    10741 tacaccacaa aagatggaaa cgtgattcag attcctgcag gaaacgaaga aggcatggaa
    10801 tcaaaagatg ctggtggatc aggcgctcct gctaaaaact aagggttaca acaaagagca
    10861 taaggtgact cgcaaacgtt acgcatgaca tgaggcagtc tgcctaagat ggtaactcac
    10921 ttatcataat gcctaaatag ccaaacacac taactcgcaa atgtacaaaa tcatataccc
    10981 gtgcttatgt gagcgaacgg ttatatgatt tttgttttcc aaaaacaaga aaagaacgct
    11041 aaattagcca gtttgtcgtg tttcaagcta ccaaaaagga agtgctaaaa tgaaaaaaag
    11101 atccattata cctgtcatct tactgttggt catcggatta atcgttggtg ttaagcgtca
    11161 tcatgactag taggtcttgc tagtgaccgt tcaaaaggga taacgttggt atggtataac
    11221 cataaaaatc gtataaaaag aaaaaatagc tattgaggag aaagtatgtt tcagttaaga
    11281 aaaaaaatga cgcgcaaaca attagccttg ttgagtgctg gagtgttgac ttgtgtggtt
    11341 ggtggtagct acttgataat gaaccatcaa caacaagaaa ttgtttctag tgtcaacaaa
    11401 gtaaaagcct taaccataaa agaagccatg gaacaaggaa aagatatcag cttgacctta
    11461 gctggcgaag taacagctaa caacagcagc aaagtcaaaa tcgactcaag taaaggagaa
    11521 gtcaaagatg tctttgtcaa aaaaggcgat gttgtcaaag taggacaacc cttgtttagc
    11581 tatgaaacgt cacagcggtt aacggctcaa agttcagaat ttgatgttca aaccaaagcc
    11641 aatcaactcc aagttgctaa aaccaatgca gcattgaagt gggaaaccta caatcgcaag
    11701 gtcaatgaaa tcaataccct aaaatctcgc tacaacactg caccagatga gagcttacta
    11761 gagcagattc gcagcgcaga agacagtgta tcccaagcac taagcgatgc caaaacagca
    11821 gatagcgatg tcaaaactgc tcaaatcgaa ctcgataaag ctaatgctac tgccacaacg
    11881 gaaaaaggta aactagagta tgacaccgtt aagtcagaca ccgcaggaac cattgttagc
    11941 ctaaatactg atttgccaaa tcaatcaaaa tccaaaaaag aaaatgaaac ttttatggaa
    12001 attatcgaca aatcaaaaat gttagtcaaa ggtaacatca gtgaatttga ccgtgacaag
    12061 ttaaaaatcg gtcaaaaagt cgaagtgatt gaccgcaaag acaactctaa aaaatggact
    12121 ggaaaagtaa cccaagttgg caacctcaaa gcagaggaaa aaggccaagg tcaaggccaa
    12181 ggtggcaatg accaacaaga caatccaaac caagcaaaat tcccttatgt tatcgaactt
    12241 gaccaatcag acaagcagcc actcattggc tcacacacct atgttaatgt gctcaacaat
    12301 gttccagaag ctggcaagat cgtattgaaa gaaaccttta caatggcaga aaatggaaaa
    12361 acctatgtgt ggaaagttga taaaaacaag gtcaaaaaac aagaaatcaa gactaagccc
    12421 ttctcaaaag gttatgttga ggtaacaagt ggcttgacca tgcaagataa gattgctcag
    12481 ccgcttcctg gcatgaaaga cggtatggag gtaggaagta ttgttaaacc ttaaagatat
    12541 tcgaaaaagc tatcatcttg gaactgaaga atttgcgatt ttaaaaggaa tcgatttaga
    12601 agttaacgag ggtgactttt tagccatcat gggaccatca ggttcgggaa agtcaacatt
    12661 gatgaatatc attgggtgtt tagataagcc tggctctggc tcatatgcca ttgaaggcag
    12721 agacgtgtca tccttatctg ataatgaact tgctgatttg cgtaatcaaa aaatcggctt
    12781 tgtttttcaa aattttaacc tgatgcccaa gctaacagct tgtcaaaatg tcgaattgcc
    12841 cttgacttat atgaatgttc ctaaaaaaga acgtcgcaaa cgagccctag agatgttaaa
    12901 gctcgtagga ttagaagaac gtagtgaatt taaaccgatg gagctatctg gtgggcaaaa
    12961 acagcgtgta gcgattgcaa gagctttagt cactaacccg agttttatcc ttggtgatga
    13021 gccaacaggt gcactagaca caaaaaccag cgtccaaatc atggacctat ttaaacaatt
    13081 caatgataac ggcaaaacga ttattatcat cacacacgag cctgaagtag ctgccttatg
    13141 caaaaagacg gtgatcctaa gagatggtaa tatagaacat tccgatatag agtaaagggg
    13201 gaaaataatg gaagatatta ttgcagcttt aggctctata ttatcacaca agatgaggtc
    13261 aattttgaca atgctaggga ttattatcgg tattggtgcc attatcgcaa tcttttcaat
    13321 catcgaaggt aataccgaaa ataccaaaag acagttgatt ggtggcagca ataacactat
    13381 taacattgtg tttaacaaaa aaagtagtat tgaccctaaa tttccagaca agtcaaatgc
    13441 taaaaaacca gactatcttc cttttatggc tgaagaagag cttagcaaaa tacagcaagt
    13501 caaaggcgtc aaaaacgccc tgatctcata tggtattgat gacaaagtct accatctagg
    13561 ccaaaaatca tctgcaaaga tttctgccat cacaaaaaac gtagctgaag tgaggcgcat
    13621 gacatttatt aaaggcagtg acttttcaga caaagacttt attgaccaaa aacaggtgat
    13681 ttaccttgaa aaaagcttgt atgagtcact atttccaaaa gacgatggcc ttggaaaatt
    13741 tgtcgaagtg atgggaaatc ccttcagggt cattggggtg tttgaatcaa aagaacaaag
    13801 tggcctcaca agtgggactg agaaaattgc ttatatcccg ctacatcagt ggtataatat
    13861 taatggtgta gtggatgcga cacctgagat tacgatccag acctatcgcg cagatgacct
    13921 caaacctgta gcaaagcgtg tcagtgatat gctcaatcaa acgatcccta aatcagatta
    13981 tatgtttggt gtcatgaacc ttaaagaatt tgaacgccaa ctagacaacc taaacaaatc
    14041 taattttgtg ttattagctg gtattgctag catctctctt attgtaggtg gtattggtgt
    14101 gatgaacatt atgctagtat cagttaccga aagaacgaga gaaattggga tcaaaaaagc
    14161 gcttggcgct agacgtaagc ttattttaaa acaattttta attgaagcgg tcattctaac
    14221 cttgctaggt ggtgtgatag gggttatttc agggatggta tcaggattga tcatcacaag
    14281 atcactcgag tatccctata tcctatcctt gttctcagtt gtcttgagtt tagcgttttg
    14341 ttgtattata gggattgtct ttggactatt accagccatt aaagcgtcta agctagaccc
    14401 aattgaagca ttgcgatttg aataatcaac caaacaagca taaaagtcaa aaaggatgac
    14461 cctttgatgt catccttagc tgcttgatac actaaacgcc aatctagaaa gggaagttat
    14521 gtttaaaata ttagtagttg aagatgatga taccatcagt caagtgattt gtgagttttt
    14581 aaaagcgaat aactatgatc cagattgtgt atttgatggc caagctgcct tagacaagtg
    14641 gcagacaacg tcttatgatc tgattatttt agacatcatg ctcccttcct taagtggctt
    14701 agaagtgcta aaaacaattc gaaagacatc agatgtccct attattatgc taacggcatt
    14761 agatgacgag tacacacagc ttgtaagctt caaccatttg attagtgatt atgtgaccaa
    14821 accattttca ccactcattt taatcaaacg tatcgaaaat gtattacgcg tctctactcc
    14881 agatgaaaag cgccaaatcg gagacttact tgttgatgag accgaacata gcgtctattg
    14941 gcaaggaaca ttggtcaaac taaccaaaaa agaatatgac attattgatt atttagcaaa
    15001 acgtcaccaa aaaattgtca ccagagacca attgatggat gacatatggg gctattctga
    15061 actggacacg cgtgtccttg acaaccacat caagaacctt cgtaaaaaaa tgacgggtat
    15121 tcccctcaag acaattacag gcatgggcta tttattggga gagcgtgagt gagactaatc
    15181 aaaaaaacct ttttggtcat caatggctta attattgtag tggttacctc tattttgtta
    15241 gtgctttatt ttgcaatgcc aatttattac accaaagtca aagacaaaga agttaagcgt
    15301 gaatttgacc aaacaagcaa gcaaatcaaa ggcaaaacgg taaccgagat tagagatatc
    15361 ctaacaaaaa aaatcaacaa agataatatt tggtacagct tagtagacag tgataaccaa
    15421 ctgctctatc cctccttgca gctcctagac ggcgtcagtg aaagcaaaga cagtcaaaat
    15481 gttaatatcg tcaccacctt tgacaactct tattctaatg ttaaggtgat gagtcaaaaa
    15541 gtaaccctac gcgatggaaa aaagatgaca ttgcttgggc aatcttcact tcaacccgta
    15601 acagatgcta gcaaagttct ccttgacctc tacccgtcac tattgatgtt ttcagtgacc
    15661 gtagggagta ttgtcgccta cttatatagt aggacttcta gccggcgtat tcttagcatg
    15721 tcacaaaccg ccaaaaaaat ggtcaaccta gaaccaaact taacgtgtac cattcacggc
    15781 aaagatgaaa tagcaatgtt ggctagtgat attaaccgtt tgtatgcgag tctttcaacg
    15841 agcatcaaat ccttgcaaaa agagtatgaa aaagcttctg attcagaaag agagaaatct
    15901 gagtttttac gcatgacatc gcacgagcta aagacaccaa tcaccagtgt tatcggaatg
    15961 attgatggaa tgctctataa tgttggtgac tttgcagatc gtgacaaata tttgcgaaaa
    16021 tgccgagacg tccttgaagg gcaagcccaa ctggttcaat ccattttatc tttatctaag
    16081 attgaaaccc tagcttctca aaatcaagaa ctgttttcac taaaaagcag tctagaagaa
    16141 gaaatggaag tctttctcgt cttatcagaa ctaaaacacc tcaaagtgac tatcaatctc
    16201 gaagaacaat tcgtcaaagc caataaagta tacctactaa aggcgattaa aaatattatt
    16261 gacaatgcct ttcactatac caaaccaggc ggccaagtga tgattcaact aaaagacaac
    16321 caactagtga ttaaaaatga agcagagaca ttattgacac aacagcagat gaaacagtta
    16381 ttccaaccgt tttatcgacc agattatagt cgtaacagga aagacggtgg tacaggattg
    16441 gggctgttta tcacccacca gattcttgat cagcatcatc tggcttatcg ctttgttgtt
    16501 cttgatcaaa gatggatggt atttacgatt gattttccat cccatcatga cgattgatcc
    16561 acactagtag gacgacaaaa gtcatcaatc actaaccaag tcttgacctt tacccatcat
    16621 tgtttaggca agtataaaaa agaggagaga aatcatatat gaagaaaagg aaattgttag
    16681 cagtaacact attaagtacc atactcttaa acagtgcagt gccattagtt gttgctgaca
    16741 cctccttgcg taataacaca tcatccactg atcagcctac tacagcagat aatgatacgg
    16801 atgacgagag tgaaacacca caaaaagata aaaaaagcaa ggaaacagcg tcgcagcacg
    16861 acacccaaaa agaccataag tcatcacaca ctcacccaac ccccccttca aatgatacta
    16921 agcagaccga tcaggcatca tctgaagcta ctgacaaacc aaataaagac aaaaacaaca
    16981 ccaagcaacc aaacagcagt gatcagtcta ccccctctcc caaagaccag tcgtctcaaa
    17041 aagagtcaca aaacaaagac ggccgaccta ccccatcacc tgatcaacaa aaagatcaga
    17101 cacctgataa aacaccagaa aaatcagctg ataaagcccc tgaaaaagga ccagaaaaag
    17161 cagttgaaaa aacaccagag ccaaatcgtg acactccaaa acccatccaa cctcctttag
    17221 cagcagcagc tcctgtcttt gcaccttgga gagaaagtga caaagacctg agcaagctaa
    17281 aaccaagcag tcgctcatca gcggcttacg tgagacactg gacaggtgac tctgcctaca
    17341 ctcacaacct gttgtcacgc cgttatggga ttactgctga acagctagat ggttttttga
    17401 acagtctagg tattcactat gataaagaac gcttaaacgg gaagcgttta ttagaatggg
    17461 aaaaactaac aggactagac gttcgagcta tcgtagctat tgcaatggca gaaagctcac
    17521 taggtactca gggagttgct aaagaaaaag gagccaatat gtttggttat ggtgcctttg
    17581 acttcaaccc aaacaatgcc aaaaaataca gcgatgaggt tgctattcgt cacatggtag
    17641 aagacaccat cattgccaac aaaaaccaaa cctttgaaag acaagacctc aaagcaaaaa
    17701 aatggtcact aggccagttg gacaccttga ttgatggtgg ggtttacttt acagatacaa
    17761 gtggcagtgg gcaaagacga gcagatatca tgaccaaact agaccaatgg atagatgatc
    17821 atggaagcac acctgagatt ccagaacatc tcaagataac ttccgggaca caatttagcg
    17881 aagtgcccat aggttataaa agaagtcagc cacaaaacgt tttaacctac aagtcagaga
    17941 cctacagctt tggccaatgc acctggtacg cctataatcg tgtcaaagag ctaggttatc
    18001 aagtcgatag gtacatgggt aacggtggcg actggcagcg caagccaggt tttgtgacca
    18061 cccataaacc taaagtgggc tatgtcgtct catttgcacc aggccaagca ggagcagatg
    18121 caacctatgg tcacgttgct gttgtagagc aaatcaaaga agatggttct atcttgattt
    18181 cagagtcaaa tgttatggga ctaggcacca tttcctatcg gacgttcaca gctgagcagg
    18241 ctagtttgtt gacctatgtc gtaggggaca aactcccaag accataaccc cacaaccaag
    18301 aagctacctg cctgttgacc aatctcaaaa agagacagcg tcaacttaga atgaggttca
    18361 tcagatgata aagaaagtga ccaccccgag tcaaaaaaca aaaaaacgtg ttcgtaatgg
    18421 ctatctccta aaactaggta cagcctgttt actcttaagt atactcagtt atggcattgg
    18481 tcttttgggc cagcctagca tggaaaatac gtttgtggga atagcgagtg ttgccatgtt
    18541 aggaagtgtc tgtttcttta ttatctttgc tctaaaccgt atctttgacg cactagagga
    18601 caatttgcga gattagagta ataggtcaaa taatcttcca ataatttaga gcacaagcta
    18661 agttattgga agtttttttg tcttttgaga gccatgatgc catcaaaaag tcaaacactc
    18721 cttatgatgt gagagtcaca tggctaaaat taaaagcaaa atagacccca aagccctgtt
    18781 ttctcaaaac agtcaacgaa aaaagacaag ttaaccagtt catgaaaaac atcgtagaaa
    18841 cattttcaag agctaatgtt ggtgtaaatt gactgaagta tgatagaatt tttaatgaat
    18901 gtgacaataa tgtcacagat aaaaaccgaa aacagcaaaa ataattaaat tgaatataaa
    18961 aaagttaatt aagtgagtga taataaagaa atgaagttca aaaaatataa ataaagtaaa
    19021 acaccagaac tactaaaagc agtgaatgac ataaatgtcg ctaaaaaaat agatagaaga
    19081 gtctaaaatt tcagttttag gtcacgtttt atgaatggaa atcaggatga tgaccttaag
    19141 ttgtgatttg cgcgtcgttg accgctttcc tcgttttaat gaaagaattt gaaagatatt
    19201 ttatggtata cttt
//
LOCUS       DAUELZ010000029        18398 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.029, whole genome shotgun sequence.
ACCESSION   DAUELZ010000029 DAUELZ010000000
VERSION     DAUELZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18398
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.029"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            80..2038
                     /gene="ligA"
                     /locus_tag="VHI09_000757"
     CDS             80..2038
                     /gene="ligA"
                     /locus_tag="VHI09_000757"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEQ6120649.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKENFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRQL"
     gene            2052..3074
                     /locus_tag="VHI09_000758"
     CDS             2052..3074
                     /locus_tag="VHI09_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ6120650.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCRKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            3482..3679
                     /locus_tag="VHI09_000759"
     CDS             3482..3679
                     /locus_tag="VHI09_000759"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEQ6120651.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            3714..4430
                     /gene="atpB"
                     /locus_tag="VHI09_000760"
     CDS             3714..4430
                     /gene="atpB"
                     /locus_tag="VHI09_000760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEQ6120652.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            4448..4942
                     /gene="atpF"
                     /locus_tag="VHI09_000761"
     CDS             4448..4942
                     /gene="atpF"
                     /locus_tag="VHI09_000761"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEQ6120653.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            4942..5478
                     /locus_tag="VHI09_000762"
     CDS             4942..5478
                     /locus_tag="VHI09_000762"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEQ6120654.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDTLQADVLAILETFETTN
                     LDQSLSSLAVPHAEKIKLLTLLERNNSVYMNNFLNLILQNEREAYLYQMLQAVLNEIA
                     IVSNQYNVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            5494..7002
                     /locus_tag="VHI09_000763"
     CDS             5494..7002
                     /locus_tag="VHI09_000763"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEQ6120655.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            7018..7893
                     /locus_tag="VHI09_000764"
     CDS             7018..7893
                     /locus_tag="VHI09_000764"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEQ6120656.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            8055..9461
                     /gene="atpD"
                     /locus_tag="VHI09_000765"
     CDS             8055..9461
                     /gene="atpD"
                     /locus_tag="VHI09_000765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEQ6120657.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDHLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            9474..9890
                     /locus_tag="VHI09_000766"
     CDS             9474..9890
                     /locus_tag="VHI09_000766"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEQ6120658.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            10156..10413
                     /locus_tag="VHI09_000767"
     CDS             10156..10413
                     /locus_tag="VHI09_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEQ6120659.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            10478..11749
                     /gene="murA"
                     /locus_tag="VHI09_000768"
     CDS             10478..11749
                     /gene="murA"
                     /locus_tag="VHI09_000768"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ6120660.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTSLHGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            11753..11941
                     /locus_tag="VHI09_000769"
     CDS             11753..11941
                     /locus_tag="VHI09_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ6120661.1"
                     /translation="MTNGLKYVLEQMLLLLVIAALACLFLAIGLMIGYSFMGDGHSPW
                     HILSMDKWAELVNKFTGK"
     gene            11977..12841
                     /locus_tag="VHI09_000770"
                     /pseudo
     CDS             11977..12841
                     /locus_tag="VHI09_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            13124..14167
                     /gene="pheS"
                     /locus_tag="VHI09_000771"
     CDS             13124..14167
                     /gene="pheS"
                     /locus_tag="VHI09_000771"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEQ6120662.1"
                     /translation="MDLQAQLEELKTKTLETLQPLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGKNISMGDLKGTLEMIIKKMFGEERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            14377..16782
                     /locus_tag="VHI09_000772"
     CDS             14377..16782
                     /locus_tag="VHI09_000772"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEQ6120663.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPAEGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIGLSITPNRADALSMRGVAHEVAAIYGKSVSFPPKNLQESDKATSEAIEVAIASDN
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDANS
                     QTVVLEVAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMISGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKENLPNEVNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLANMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALSSDQTFVEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTKIKLFDVYEGVTIQAGKKSMAYSLNFQNPNDNLTDEEVAKYMEKITKALTEQVGA
                     EVR"
     gene            16892..17269
                     /locus_tag="VHI09_000773"
     CDS             16892..17269
                     /locus_tag="VHI09_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEQ6120664.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVTVTITLEGLSHV"
     gene            17262..17648
                     /locus_tag="VHI09_000774"
     CDS             17262..17648
                     /locus_tag="VHI09_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEQ6120665.1"
                     /translation="MSDASLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKKHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            17721..>18398
                     /locus_tag="VHI09_000775"
     CDS             17721..>18398
                     /locus_tag="VHI09_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6120666.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSVLLAKDADATLTLSQVSRAQENQITADKVAPLAQLNTVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGGPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTF"
BASE COUNT         5608 a         3238 c         4067 g         5485 t
ORIGIN      
        1 ggagtggcca acgattactt ttgatgttgt tatgatatag aaatagctca tttgttatct
       61 tttatgataa aataaaagta tgaaaaaacg cataaaagaa ttaacagatc ttcttaaccg
      121 ttaccgttat gactattata ctaaagatgc cccatcagtt tctgacagtg actatgataa
      181 gctttatcgt gaactggtga ctttagagca gtcatatccc gagtatgtct tgcaagatag
      241 cccaacgcag caggttggag ggactatttt aaaggggttt gaaaaatatc gacatcagta
      301 tccgcttttt agtttgcagg atgctttttc acgtgaggag ctggatgctt ttgataagcg
      361 cgtcaaggcc gaatttccga atgccaccta tctagcagag ttaaaaattg atggtttatc
      421 tatttccttg agttatgaga atggcttctt acaggttgga gcaacacggg gtgatggcaa
      481 tatcggtgaa aatatcacag aaaatatcaa aaaaataaag gatattcctc accaattgag
      541 tgagccttta accatcaccg ttcgaggaga ggcttacatg tctcgtcagt cctttaaagc
      601 gatcaatgaa gcacgccaag aaaatgggga gactgaattt gctaatccta gaaatgctgc
      661 cgctgggaca ctaaggcagc tagatacggc agtggttgct aagcgtcaac tagccacttt
      721 tttgtatcaa gaagctagtc cgacagctag aaatcaacaa aatgaggtct tagcagaatt
      781 agcagatcta ggtttttcag taaatccgta ttatcaactc acatcgtcta tggatgagat
      841 ttgggatttt atcaaaacta ttgaggcaaa aagagatcaa ttagcttatg acatagatgg
      901 cgttgttatt aaagttaata gcctagccat gcaagaagag ctagggttta cggttaaagc
      961 tccacgctgg gccattgctt acaaatttcc agcagaagaa aaagaggcag aaatcctttc
     1021 ggttgactgg acagtgggac gaacaggcgt ggtcacacct acagcgaatc taactcctgt
     1081 gcaactagcg gggacaaccg tgagtcgtgc aaccctacat aatgtggatt atattgccga
     1141 aaaagatatc cgtattggcg atacagtgat tgtatataag gcaggggaca ttattcctgc
     1201 tgtcttaaat gtcgttatga gtaagcggaa ccagcaagaa gtcatgctta ttcctaaact
     1261 atgcccatct tgtggtagtg aattagttca ttttgaggat gaggttgctt tgcgttgtat
     1321 taatccgttg tgtcctagtt tgattcagcg aagtttggag cattttgcaa gtcgtgatgc
     1381 catgaacatt actgggctgg gaccagctat tgtggaaaaa ctatttttag cggggtttgt
     1441 tcatgatgtt gctgatattt accagttgac caaagaaaat ttcatgcaat tagatggcat
     1501 caaagaaaag tcagctgata aattgctcgc tgcgattgag gcttctaaat caaattcagc
     1561 tgaaaaactg ttatttggtc taggaattcg tcatattggc tctaaagtta gtcgcttaat
     1621 tttagaggta tacggagata tttctgctct tttaaccgct aaagaagagg aaatcgcaag
     1681 gatagacggc cttggctcaa cgattgctca atcacttact caatactttg aacaaaaaac
     1741 ggctgcgatt ttagtagacg agttaaagac agcaggtgtc aacatgcatt actcaggtca
     1801 aaaagttaat agtgacgcag cgctttttgg tctaactgtt gttttaactg gcaaattaaa
     1861 tcagctgaac cgaaatgagg ccaaagataa attagaggca ttaggtgcca aagttactgg
     1921 aagtgtatct aagaaaacag atttagttat tgcaggtagt gacgctggtt ctaagctaga
     1981 aaaagcaaag agtttaggaa ttcggattga agatgaagat tggctacgcc agctttaagt
     2041 gaggtaatgt tatgaaaaaa caattgagag cacgtctcat ttataatcca acgtcaggac
     2101 aagaacttat gagaaaaagt gttcctgaag ttttagacat attagaaggg ttcgggtatg
     2161 aaacatctgc ctttcaaaca acagcccaaa agaactctgc tctaaatgaa gcaagaagag
     2221 cggcaaaggc aggttttgac ttattgattg ctgctggagg tgatggcact attaacgaag
     2281 tggtcaatgg aattgctccc ttgaaaaagc gccctaaaat ggccattatt ccaacaggta
     2341 ccactaatga ttttgctaga gcgttaaaag taccaagagg aaatccgagc caagcagcaa
     2401 aacttatcgg aaaaaatcag actatccaga tggatattgg tagagctaag aaagatactt
     2461 atttcatcaa tattgctgca gcaggaagtt tgacggaatt aacttacagt gtaccaagtc
     2521 agttaaaaac gatgtttgga tacttggctt atcttgctaa aggagtagaa ctgctcccaa
     2581 gagttagcaa tgtgcctgtt aaaattacgc atgacaaagg cgtctttgaa ggtcaagttt
     2641 ctatgatttt tgcagctatt accaattctg ttgggggatt tgaaatgatt gctcctgatg
     2701 caaaactaga tgacggtatg tttacgctca ttttaataaa gacagcaaat ctttttgaaa
     2761 ttgtacatct tttgcgtttg attttggatg gtggtaagca tattacagat cgccgtgtcg
     2821 aatacattaa gaccagtaaa atagtcattg aaccccaatg tcgtaagcga atgatgatta
     2881 atttagatgg cgagtacgga ggagatgctc caattacatt agagaaccta aaaaatcata
     2941 tcactttttt tgcagatact gatttgattt ctgatgacgc tttagtctta gatcaggacg
     3001 aattggaaat tgaggaaatc gtaaagaaat ttgctcatga agtagaagac ttagaacaag
     3061 aattagaaga ataatacaat agttattagc tactgacagt ttagtcaaat gcactactat
     3121 tagaatctat tatttttata atctaagatt tttaaatcaa ttgaggtagt gttttttgat
     3181 gcactcaaag agtcaattta ttcatatgag gagtccattt gaggtctctc ttttttgatc
     3241 atatgcaaga aaatgccatg ttttaggggt gttattatac cctttttgga atcttgttgc
     3301 ctttttgtta taataagagt gtaattctat ttttaaaaag atcttaacaa agacagactc
     3361 gtttatcaaa aaggttagta ttagtctaag aaagcgtact cagtctaaag aaatctgttg
     3421 acattcttaa ttttaaggaa tagaatagca aagtaaaaaa aatttaaagg agtattctac
     3481 tatgaatcct attttcgcat tggctttggc ctgttttggt gtttctcttg ccgaaggttt
     3541 cttgatggca aatcttttta aagcagcatc tcgccaacca gaaatcattg gtcaattacg
     3601 ttcattaatg atcttgggtg ttgcctttat cgaaggtact ttctttgtta cccttgttat
     3661 ggccttcatt cttaaataac agcctatttg ataatagaaa aggagggtgt tagatggaag
     3721 aagctaaaat acccatgcta aagcttggtc caataacctt taatttgacc ctacttgctg
     3781 tttgtattgt cacaattgct gttatctttg cctttgtttt ttgggcaagt cgccaaatga
     3841 aattgaaacc agaagggaag caaactgctt tagaatattt aatcagtttt gtggatggta
     3901 ttggagaaga gcacttagat cataatctac aaaaatctta ttcgctgtta ctctttacca
     3961 tttttctctt tgtggctgtc gctaataatt tagggttatt tactaagtta gagacagtta
     4021 atggctataa cctatggacc tcgccaacag ccaatctggc ttttgacctt gctctatctc
     4081 tttttattac cttaatggta cacattgaag gggttagacg tcgtggcttg gttgcccatt
     4141 tgaaacgttt ggctacaccg tggccaatga ctccgatgaa tttattagaa gagttcacaa
     4201 atttcttatc acttgccatt aggttattcg gtaatatctt tgccggggaa gttgttacag
     4261 gtttgattgt tcaactggcc aattatcgag tttattggtg gccgatcgct ttcctagtca
     4321 atatggcgtg gacagccttt tcagtcttta tttcctgcat acaggctttt gttttcacaa
     4381 aactaacagc gacctatcta ggaaagaaag ttaatgaatc agaagaataa ggaaggagaa
     4441 caaacgaatg tcaatcacat ttggtgaact cgttgggaat tttattctag taacaggttc
     4501 tgtaatcgtc ttgttactct tgatcaaaaa attcgcttgg ggtgcaattg aatcgatttt
     4561 acagacacgc tcacaacaaa tctctcgaga tattgatcag gctgagcaat cacgtctaag
     4621 tgctcaacag ttagaggcaa aaagtcaagc taacctagat gctagtcgtt cacaagcaag
     4681 taaaatcatt agtgatgcca aagaaattgg tcaattacaa ggtgataaat tggtggcaga
     4741 agctactgat gaagcaaaac gcttgaaaga aaaagcgttg acagatattg aacaaagcaa
     4801 atcagacgct atttcagcag tcaaaacaga aatgtctgat ttaacggttc ttttagcgga
     4861 aaaaattatg ggagccaatc ttgataagac ggcgcaaagc cagcttattg acagttatct
     4921 tgatgactta ggagaagctt aatgaccaaa aaagaacagg ctcttatcga gcaatatgca
     4981 aaaagccttg ttgaggtagc aagtgaacat cattctcttg ataccttaca agctgatgtg
     5041 cttgcgattt tagagacgtt tgaaacaact aatcttgatc aaagtctatc aagtctagct
     5101 gttccgcatg ctgaaaaaat aaaattgcta accttattag agagaaataa ttctgtttat
     5161 atgaataatt tcttgaatct cattttacaa aacgaacgtg aagcttattt gtatcaaatg
     5221 cttcaggctg ttttaaatga aattgcaata gtttcaaatc agtataatgt gactgttacc
     5281 tcatcactac ctttgactga ggagcaaaaa agccgtgttc gagcagttgt tgcaaagaaa
     5341 tttgctgtaa cagcgggtcg tttaattgaa aaagtagatc cttcgcttat tggaggattt
     5401 attatcagcg tcaataataa agtaattgat accagtattc gtcgtcaatt gcaagcattt
     5461 aaaatgaatt taaaatagaa agtggtgtga cttttggcaa ttaatgcaca agaaattagc
     5521 gctttaatta aaaagcaaat tgagaacttc cagccaaatt tcgatgtcac agaaactggg
     5581 atcgttacct atattggtga cggtattgca cgtgcccgtg gattagataa tgccatgagt
     5641 ggtgaacttc ttgaatttga aaatggtgcc tatgggatgg ctcaaaacct tgagtcaaat
     5701 gatgtaggta ttattattct tggtgatttt tcagcaatcc gtgaaggaga tgtggttaag
     5761 cgcaccggta aaatcatgga agtaccagtt ggtgaagctc tcattggacg tgtggtaaac
     5821 ccacttggac aaccagttga tggcttggga gatattgaaa caactggctt tagaccagtc
     5881 gaaacgccag ctcctggtgt tatgcagcgt aaatccgttt ctgaaccctt acaaactgga
     5941 ttaaaagcaa ttgatgcttt ggttccaatt ggtcgtgggc aacgtgagtt gattatcgga
     6001 gaccgtcaaa caggaaaaac atccgtagct attgatgcga ttttgaacca aaaaggtcaa
     6061 gacatgattt gtatctatgt ggctattggt caaaaagaat caaccgttcg tacacaagtt
     6121 gaaacccttc gccgttacgg tgccttggat tatactattg ttgtgacagc gtcagcctca
     6181 caaccatcac cattgctctt catcgctcct tatgcgggtg ttgccatggc tgaagaattt
     6241 atgtaccaag gcaagcatgt cttgattgtc tatgatgatt tatcaaaaca agcggtggct
     6301 tatcgtgaat tgtctctctt gcttcgtcgt ccaccaggtc gtgaagctta cccaggggat
     6361 gttttctact tgcatagccg tcttttggaa cgctctgcaa aagtttctga tgacttaggt
     6421 ggaggttcta tcacagctct accatttatc gaaacacagg ccggagatat ctctgcatat
     6481 attgcgacta acgttatttc gattacagac ggacaaatct tcttgcaaga gaacctcttt
     6541 aattcaggaa ttcgtccagc gattgatgca ggatcatctg tctcacgtgt aggtggttca
     6601 gctcaaatta aagcgatgaa aaaggttgct ggtactcttc gtttggattt ggcttcctat
     6661 cgtgaattag aagcctttac ccaatttggt tcggaccttg atgctgcaac acaagcaaaa
     6721 cttaatcgtg gtcgccgtac ggttgaaatc ttgaagcaac cattgcataa accgttacct
     6781 gttgaaaagc aagtcgttat tctttatgcc cttactcatg gtttcttaga tgatgttcct
     6841 gtagatgata ttttggcttt tgaagaagct ctttacgact actttgacgt tcattacaat
     6901 gatttatttg aaactatccg taccacaaaa gaccttcctg aagaagctgc ccttgatgcc
     6961 gctattaaag cgtttaaaga acactcaaac tttaagtcat agagggagga tactagtatg
     7021 gcaggctctc taagtgaaat taaagctaaa attatttcaa ctgagaaaac aagtaaaatc
     7081 actagtgcca tgcgtatggt ttcttctgca aaacttgtta aatctgagca agcagctcgt
     7141 gattttcaaa tttacgcctc aaaaattcgc cagattacta cagatttatt gaaatcagaa
     7201 ttaaccatag gatctgataa tccgatgttg gtgtctcgtc ctgtcaaaaa aacaggctat
     7261 attgtcatca cgtctgataa aggacttgtt ggaggttaca attcaaaaat cttgaaatct
     7321 gttatggata tgattacaga gtaccatgct gatggtgact atgaaatcat ttctattggt
     7381 agtgttggtt cagacttctt caaagcaaga gggatgaatg tggcttttga acttcgtgga
     7441 cttgcggatc aaccaagttt tgaacaagtg agacaaatca tttcacaatc cgttgacatg
     7501 tttgtcaatg agatttttga tgagttatat gtctgttaca atcaccatgt gaatagctta
     7561 accagccaag tccgtgttca acagatgttg ccaatttctg acttggttgc tgatgaagca
     7621 gcagaagaag gtgttactgg gtttgaattg gaacctaatc gtcatgatat tttagatcaa
     7681 ttgttacctc aattcacaga aagtttaatt tatggggcta tcattgatgc taaaacggct
     7741 gaacatgcag caggtatgac tgctatgcag acagcgacag ataacgctaa gaatgtcatt
     7801 aacgatttga ccatccaata taaccgagct cgtcaagcag caattacaca agaaattact
     7861 gaaattgttg caggagcaaa tgcactagaa taaaaaataa cgctgactag cgattaggtt
     7921 attgtcagca ttcaatttta tcaatcgaga taaaatgact tatgggactt ttctattgaa
     7981 agactgctct tttcagtata aaagaagtta gcaagactaa ctaagccctg actatcgaat
     8041 ataaggagaa ataaatgagc tcaggcaaaa ttgctcaggt tgttggtcca gtagtagacg
     8101 ttatgtttgc tagtggggac aaacttccag agattaataa tgcattgatt gtttataaag
     8161 atagtgataa aaagcaaaaa atcgtccttg aagttgctct ggaacttggt gacggtatgg
     8221 tgcgaacaat cgctatggaa tcaactgatg ggcttacacg tgggttagaa gttcttgaca
     8281 ctggtcgtgc gattagcgta ccagtaggta aagaaacttt gggacgcgtc tttaatgtac
     8341 ttggagaaac cattgatttg gaagaaccat ttgcagaaga tgttgaccgt cagccgatcc
     8401 ataaaaaagc accatcgttt gatgagttat caacatcatc agaaattctt gaaactggta
     8461 tcaaggtaat tgaccttctt gccccttacc ttaaaggtgg taaagttgga cttttcggtg
     8521 gtgccggagt tggtaagact gtccttatcc aagaattaat ccacaatatc gcccaagaac
     8581 acggaggtat ttcagtattt accggtgttg gtgagcgaac acgtgaagga aatgaccttt
     8641 actgggaaat gaaagaatca ggcgttattg agaaaactgc catggttttt ggtcagatga
     8701 atgagccgcc tggggcacgt atgcgtgtag cccttactgg tttaaccatt gctgagtatt
     8761 tccgtgatgt agaaggccaa gatgttttgc tctttattga taatatcttc cgtttcactc
     8821 aggcaggttc agaagtatca gccctcttag gccgtatgcc ttctgctgtt ggttaccaac
     8881 caacacttgc tactgaaatg gggcaattgc aagaacggat tacgtcaact caaaaaggtt
     8941 ctgttacatc tatccaagcg atttatgtac cagcagatga ttatactgac cctgcgccag
     9001 caacagcatt tgctcacttg gattcgacaa ctaaccttga acgtaaattg actcaaatgg
     9061 gtatttaccc tgcggttgac ccattggctt caagttcacg tgctttatca cctgagattg
     9121 ttggtgaaga gcactatgct gtggcaacag aagttcaacg tgtgcttcaa cgctaccgtg
     9181 aattacaaga tattattgct attttgggga tggatgaatt gtcagatgag gaaaaaacat
     9241 tggttggacg tgcgcgtcgt atccagttct tcctctcaca aaattttaac gttgctgaac
     9301 aatttaccgg tcttcctggt tcttacgtgc cagttgctga gacagttcgc ggctttaaag
     9361 aaattcttga aggaaaatac gatcaccttc cagaagacgc ctttcgttca gttggtccaa
     9421 ttgaggatgt gattaaaaaa gccgaaaaaa tgggctttta gtgaggtgat gccatgacac
     9481 aaatgactgt tcaagtggta acgccagatg gaatcaaata tgatcaccat gctaaatgta
     9541 tttctgtaac aacaccagat ggggaaatgg ggattttacc taatcacatc aatttaattg
     9601 cacctttgca agtccatgaa atgaaaattc gccgaggtgg tgaagatgaa aaagtggact
     9661 ggattgctat caatggtggt attattgaaa tcaaagataa tgtggttacc gttgtcgctg
     9721 attctgcaga acgtgaccgt gatatcgatg tcagccgagc agagcgagca aaactaagag
     9781 cagaacgtga aattgcacag gctgaaacga cccacaacat tgatgaagtt cgtcgtgcca
     9841 aggttgcctt gcgccgcgct ttgaatcgta tcaacgttag taaaaaataa gtggtgttga
     9901 taaaagacaa ggataagtaa gaaaggtttt taacctatta ttttgtttgc tgattctttt
     9961 taacatcagt ttctgagagt ttgaccatac taccttctct aaacacgtta ttattgttga
    10021 gagaagttat atgggggaac tcttttttgt atccacgtta ttagtagaaa ctcagtaatg
    10081 acgcttaata tatttttagg ctatcaactt agtcagagaa gatgtcaact taattgataa
    10141 gttggacgga tcaagatggt atgttataat aatcctatgg aattattaat gaagatcatt
    10201 gccttttcgt gtcatttatt ttttattggc cttagttacc aattattgat tagtgtgatt
    10261 gactggacaa aatttagtca ttatcatcca gaaaatcttg gccggctacg actttttgtc
    10321 tttttattag ctactgccct agggtatttg gtgagccatt gtatgctgga attgcttcaa
    10381 attagccaga cgctcttttt tgagttccgc taatactgga ataaaagaag tatggtataa
    10441 ttttactacg actaagagat agataaggga gaaaattgtg gataaaataa tcattgaagg
    10501 tggtcaaaca cgcttagaag gagaagttgt tattgagggt gctaaaaacg cagttcttcc
    10561 tttactggca gcatctattt taccttcaaa aggaaaaaca attcttagaa acgttcctat
    10621 tctatctgat gtttttacta tgaataacgt ggtacgtggt ttagatattc gtgttgattt
    10681 taatgaagca gctaacgaaa tcacagttga tgcctcagga cacattttag atgaagcccc
    10741 ttacgagtac gtgagccaaa tgcgtgcctc tattgttgtt ttaggaccaa ttttggcacg
    10801 taatggccac gcaaaagtat caatgcctgg aggctgtacg attggcagta ggcctattaa
    10861 tttgcactta aaaggattag aggctatggg ggcaacgatt actcaaaaag gtggagatat
    10921 taccgctcaa gcagatcgac tgcaaggtgc catgatttat atggacttcc catctgtagg
    10981 agctacccaa aacttgatga tggctgccac tttagctgat ggtgtgacca ctattgaaaa
    11041 tgctgctcgt gagcctgaaa ttgttgattt agctcaattt ttgaataaaa tgggagctag
    11101 aattcgtggt gctggaacag agacgttaac gataacaggc gtcacaagtc ttcatggggt
    11161 ggagcacgat gtggttcaag accgtattga agcaggtact tttatggttg cagcggcgat
    11221 gacttcaggc aatgttttga ttagagatgc tgtttgggaa cataatcgtc cattgatttc
    11281 aaaattaatg gaaatgggtg tttctgtaac agaagaagaa tatggtattc gcgttcaagc
    11341 aaacacgcca aagctcaaac ctgtaacggt taaaaccttg cctcatccag gttttccaac
    11401 agacatgcaa gctcaattta cagctttaat ggcagtggtc aatggagagt ctactatggt
    11461 tgaaactgtc tttgaaaatc gatttcaaca tctggaagaa atgcgacgca tgggcttaca
    11521 atctgaaatc cttagagaaa ctgccatgat tcatggtgga agacaactgc aaggtgctcc
    11581 tgtgatgtca acagatttac gtgccagtgc tgcccttatc ttgacaggga ttgttgctca
    11641 aggagttaca atagttaata atttggttca tttagatcgt ggctactatc aatttcatga
    11701 aaaattagca aagcttggag ctactattag tcgtagcagt gaggtctagc ttatgacaaa
    11761 tggtttaaaa tatgtcttag aacaaatgtt attactttta gtaattgctg ccttggcgtg
    11821 tcttttttta gctattggac ttatgattgg ttatagtttt atgggagatg gccatagtcc
    11881 atggcatatt ttatctatgg ataagtgggc agagttagtg aataaattta ctggaaagta
    11941 ggccttgcct actttttata gcatttgaga ggaagtatgt taacaaggaa aaaacgaaaa
    12001 agggtgctta attccttctt attgattttg ttagtgttgg ggtctaactg ccttgctaag
    12061 agtgatttca tagctagcaa tcatcctatc aagcaacttt atcagactct tgttggggag
    12121 aagaaaaaat cattactcac acataataat ttgcccgctg atgttcccac agaagctctg
    12181 gcggcatcgg tattaacacc atcaatcaaa aagcaactag gtccaaatgt tcagtggaat
    12241 ggagcaggcg cctatattat caacaggaac aaaacagatt taactgctaa ggtagccagt
    12301 ccgccttatg ccaacaatga aaccaaatgg gtgcaagagc aaaaggttcc tactagagct
    12361 aatgccttac ttactaagtc gacacgacag tatcgaaatc gctatcaaac gaacaatgct
    12421 tatcgtacct ttaagccagc aggttggcac cgattgcacc acttaaaagg aagttatgat
    12481 cacgcggttg atagagggca tttgctaggt tatgctttag ttggtggact gaaaggtttt
    12541 gatgcttacc agtaatcctg ataatattgc cactcaacta agttgggcta atcaagtaaa
    12601 taaaccctac tcgacaggtc agaactacta tgaaggttta gtacgtcgtg ctttggataa
    12661 ggggcatcgc gtccgctacc gtgtcaccct actttatgat ggtgataatc tactagccag
    12721 cggtagccat ttggaggcta aatcctctga tgacagcttg acttttaacg tgtttgttcc
    12781 aaatgtccaa ccaggattga ccgttgacta tcggacggga caaatagcta tcaatctcta
    12841 agcgaaaagt cactgattta agattgtcac taagcacttt ttttgataga atagaactaa
    12901 ttgaataagt gtctatgaga ctagtactaa tagagaaatt aacagggagt gagggccgtg
    12961 actggaagcc ttcttaaggg aactattagg aattcactca gctaaagaca attaattaag
    13021 gtctgataac atcagataaa aacggatggt accgcgtgtc aacgctccgc ttatcaagtg
    13081 gaggagtggc acgctttttt catcgctaaa agaagggaaa actatggatt tacaagcaca
    13141 attagaggaa ttaaaaacaa aaacattgga gaccttacag cctttgacgg gtaatcatac
    13201 caaagaactg caagatttgc gcgtggctgt tcttgggaaa aagggatctt tgacagaatt
    13261 actaaagggg ttaaaagact tgtctaatga cttgcgtcct gtggttggta aacaggtgaa
    13321 cgaagttcgt gacttattga caaaagcctt tgaagaacag gctaaaattg tagaagcggc
    13381 taaaattcag gcccaacttg atgctgaaag cattgatgtg actttaccgg gtcgtcaaat
    13441 gactctgggg catcgtcatg tgttaacaca aaccagtgaa gaaatcgaag atattttctt
    13501 aggaatgggt tttcagattg ttgatggctt tgaggtggaa aaagattatt acaactttga
    13561 gcgcatgaat ttgccaaagg atcaccctgc ccgtgacatg caagatacgt tttatatcac
    13621 agaagaaatc ttgttacgga ctcacaccag tcctgtgcag gcccgcacac ttgatcaaca
    13681 tgatttttca aaagggcctt taaaaatggt ttctccaggt cgtgttttcc gtcgagacac
    13741 cgatgatgct acccactcac atcaattcca tcaaattgaa ggtttggttg tgggcaaaaa
    13801 tatttccatg ggagacctca aagggactct tgaaatgatc atcaagaaga tgttcggtga
    13861 agaacgtagc attcgtttgc gcccgtcata tttcccgttc acagaaccat ctgttgaagt
    13921 ggatgtttct tgctttaaat gtggtgggaa aggctgtaat gtctgcaaga aaacaggatg
    13981 gattgaaatt cttggtgctg gtatggttca cccaagtgta ttggaaatgt caggtgttga
    14041 tgcaaaagag tattcaggct ttgcctttgg tttaggtcaa gagcggattg ccatgttgcg
    14101 ttatggcatt aatgacattc gtggtttcta tcaaggcgac caacgtttct ctgagcaatt
    14161 taactaaaac gagtcgtttg aagacctctg tcagctattt tatctaagat tgagtgaaag
    14221 ttgataggta atgataatag gttaattgtc taaggggaaa gtccctctcg cttcaacgtc
    14281 ataagggttt tgacttggaa cttaaaatac gaaaggaaat gactcaaatc acggcttgag
    14341 agccttctct agccgagtga tttgggcact aaaattatgt tagtatctta taaatggtta
    14401 aaagaattag tagatatcga tgtgacacca gcagcacttg ctgagaaaat gtcgacaacg
    14461 ggtatcgagg tagaaggaat tgaagtccct gcagaaggtt tatcaaaact ggttgtgggg
    14521 catgtgcttt cttgtgaaga tgtgccagag acacatttgc atttgtgtca ggtagataca
    14581 ggtgatgaaa cgccacgtca aattgtgtgt ggcgcaccaa atgtgaaagc agggattaag
    14641 gttatcgtgg ctgtgccagg tgctcgtatt gcggataact ataaaatcaa aaaaggcaaa
    14701 atccgcggca tggaatctct tggtatgatt tgttccttgc aagaacttgg tttgtcagat
    14761 agtatcattc caaaagaatt ttcagacggt attcaaatct tgccagagga ggctgtgcca
    14821 ggtgatgcta tttttaaata cctggacttg gacgatcata tcattggatt gtccattaca
    14881 cctaaccgtg ctgatgcgct ttctatgcga ggtgtggcac atgaagtcgc agctatttat
    14941 ggcaagtcag tatcattccc cccaaaaaat ctacaagaat ctgataaggc aacttcagaa
    15001 gccattgaag tggccattgc ctctgataac gttctaactt acgctagccg tgtcgttgaa
    15061 aatgtcaaag ttaagccaag tccacaatgg ttgcaaaacc ttttaatgaa tgccggtatt
    15121 cgtcctatta ataatgtggt tgatgtgacc aactatgtgc tcttgtattt tggtcaacct
    15181 atgcatgctt ttgattatga taaatttgaa gaccataaga ttgtggcgcg tgcggctcgt
    15241 caaggggaaa gtttagtcac acttgatggt gaaaaacggg atttgaccac tgaggatctt
    15301 gttattaccg tggctgacaa accagttgct ttggcaggtg ttatgggtgg tcaagccact
    15361 gaaattgatg ccaactctca gacggttgtt cttgaagttg ctgtttttga tggcaaatcg
    15421 attcgtaaaa ccagtggtcg tttaaacctt cgttcggaat cctcttcacg ttttgaaaaa
    15481 ggggttaact atgcaactgt tttagaagcg cttgactttg cagcggctat gttacaagaa
    15541 ttagcagaag gtcaagtctt atcaggccat gttcaagctg gacaattgcc aacagaacct
    15601 gttgaagtct caactagcct tgactatgtg aatgttcgtt taggaacaga attaactttt
    15661 gctgatattc aaaggatttt tgaccaactt ggatttggtc taacaggaga tgaaacaagc
    15721 ttcacggtag ctgttcctcg gcgtcgttgg gatgttagca tcccagctga cttggtggaa
    15781 gaaattgcgc gtatctatgg ttatgataag ttgccgacaa ccttaccaga ggcaggggga
    15841 acggctgcag aattaacgcc aacacaggct cttcgtcgta aagtccgtgg tttagcggaa
    15901 ggattgggct tgactgaaat catttcgtat gccttgacaa caccagagaa agctgttgag
    15961 tttgcagtgg caccaagtca cctaacagaa ctcatgtggc caatgtctgt ggaacgttca
    16021 gcgcttcgtc aaaatatgat ttcagggatg ctagatacgg ttgcctataa tgtggctcgt
    16081 aaacaaagta acttggctct ttatgaaatt ggaaaaatct ttgaacaaga agctaatcca
    16141 aaagaaaacc ttcctaatga agtgaaccat tttgcttttg ctatttgtgg cttagtggca
    16201 caaaaagatt tccaaaccca agctcaggca gtggattttt accatgctaa aggtatcttg
    16261 gatactttat ttgcaaacct taacctcaag gttcaatatg tgccaaccaa agacctagca
    16321 aacatgcacc caggacggac agcgcttatc ctattagatg agcaagtgat tggatttgtt
    16381 ggtcaagttc atccaggaac agcaaaggct tatagtattc cagaaaccta tgtggcagaa
    16441 ttggatatgg ctgctttaga agcagcgttg tcaagtgatc aaacttttgt ggagattacc
    16501 aaattccctg ctatgacacg tgatgttgcc ttgttacttg accgtgaagt gagtcatcag
    16561 gccattgtaa cagctattga atcagcaggt gtaaaacgct tgactaagat taagctcttt
    16621 gacgtgtatg aaggagtaac tattcaggct ggcaaaaaat caatggctta tagccttaat
    16681 ttccaaaatc caaatgacaa tttaacggat gaagaagttg ctaaatacat ggagaaaatc
    16741 accaaagcat tgacagaaca agtcggtgct gaagtacgct aagaaaaaaa ctgggcctct
    16801 aaggctccgt ttttttctgt tataatagag gaaaaaagaa ctccagttca aatgattgga
    16861 gaaaaacatc agaaggtgga ggaaaatcag aatgtatcaa acaaaaataa caggcaaccg
    16921 cctttaccac acggtttcga aaggctacgg agatagtgtg accttatttg gaaaaactga
    16981 tcaaggggaa acccctatga gtttactggt tattgccttg tcttcttgtg tgactatgtg
    17041 tgtgcagggt ttttaccatc gtcaatttca gcaagacaca cttgctgttt ctgttgaggc
    17101 tagttatgag gaagaagttt ttgaactact tgttaggatt gatgatgttt tagatgacaa
    17161 aaagaaagct gatttattgg cttacattgg aacttattgt cgtgtgaaac aactcctcaa
    17221 acctgaagtt acggtgacca ttacgttgga aggactgtct catgtctgat gctagtttga
    17281 aaacttacca aaaacaatgg gcttatcaaa agtattgggt catggcacac tcacagcagc
    17341 attacaatgc tttaagagaa ttgtttaaag gaaatcagtg gtcagaagaa aaggtgttga
    17401 cttttcattg tttgattgaa gaagcgcaag ccattcctcc cacagtaaaa agcttacgaa
    17461 cagcatatca gcatgtttgg gggtatttta aaaaggtagc gagtcaggaa gaaaaaaaac
    17521 atttcaaaga tttggatgct cagttggaaa ctaaatctga ggagatgcta tgctttttac
    17581 aagagatgac tgctcattat cagccctctt atctgctatc ttgccgtttg attaccaaag
    17641 gtccttaaat gctaaacaga aaagtaggga tttctgggaa gttatgctaa gctagtgata
    17701 taaataaggg ggaatgagag atgtttttag cgcttaatga aatgaaacaa tccaaattgc
    17761 ggtatggttt gattgctggt ttgctatgtt tggttgccta tttgatgttc tttttgtcag
    17821 gattggcctt tggtttgatg caggagaacc gctcggcggt tgatctttgg aaagctgaca
    17881 gtgttttact agccaaggat gctgacgcta ccttgacctt atcacaggtg tccagagctc
    17941 aagaaaatca aataacagca gacaaggtag ctcctcttgc tcaactcaat accgtggcgt
    18001 ggtcagttaa aaatcctaag gatgccgaca aagttaaggt tagccttttc gggattgatt
    18061 ctaatagctt tattcgtcct aacattgtaa aaggtcgatt atttaagact aacaaagagg
    18121 ttgttttgga tcaaagcctt gcaaaagagg aagcttttgc gattggcaag gacttttaca
    18181 catcgagttc tagtcaagca ttaactatcg ttggttatac tcaaaatgct agatttagtg
    18241 ttgcaccagt ggtttatatg aatttggaag cttttgaaac attaaaatat ggaggaccac
    18301 taccaaaaga taagcaagtt gttaatgctt ttatcactaa aggaagttta acagattatc
    18361 ctaaaaaaga cttccaaaaa ttagatatta aaaccttt
//
LOCUS       DAUELZ010000030        17750 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.030, whole genome shotgun sequence.
ACCESSION   DAUELZ010000030 DAUELZ010000000
VERSION     DAUELZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17750
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.030"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            61..2235
                     /locus_tag="VHI09_000776"
     CDS             61..2235
                     /locus_tag="VHI09_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ6120667.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTAFVDVVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            2235..2975
                     /locus_tag="VHI09_000777"
     CDS             2235..2975
                     /locus_tag="VHI09_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120668.1"
                     /translation="MTELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVMVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEVAKKHGMALLEKVGLSDKADALPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(3123..3275)
                     /locus_tag="VHI09_000778"
     CDS             complement(3123..3275)
                     /locus_tag="VHI09_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120669.1"
                     /translation="MTEKNGVTFLMAVGFFLIAISPMVGNKYLHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(3272..4651)
                     /locus_tag="VHI09_000779"
     CDS             complement(3272..4651)
                     /locus_tag="VHI09_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ6120670.1"
                     /translation="MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPIFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(4829..5278)
                     /locus_tag="VHI09_000780"
     CDS             complement(4829..5278)
                     /locus_tag="VHI09_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEQ6120671.1"
                     /translation="MIISAEGDVSIHHCFEAMLISLQEDYRQNTLRQLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYRLEALGENRTRITYSYD
                     YHPLDIFQKANHFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            complement(5275..5610)
                     /locus_tag="VHI09_000781"
     CDS             complement(5275..5610)
                     /locus_tag="VHI09_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ6120672.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            complement(5613..5924)
                     /locus_tag="VHI09_000782"
     CDS             complement(5613..5924)
                     /locus_tag="VHI09_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ6120673.1"
                     /translation="MKSIMLVCNAGMSTSVLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDFLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(5947..7941)
                     /locus_tag="VHI09_000783"
     CDS             complement(5947..7941)
                     /locus_tag="VHI09_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ6120674.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVTQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNTFLEGINFAEIMIIVLDECRDAKLKLSDFVM
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYQVEEVMGIRFPKEEA
                     NYIALHLNVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLTTRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQNNDVSDDEWAYISL
                     HLMAAIERYSNRHKLRVLVVCATGYGSAMMLKNRLEREFEGRLRIVDVISYYEITEER
                     LKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMS
                     LAKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFYHQMVLREN
                     YSPVIYGEVLAFPHPANPMTYSEQVVVAICREPLEWDKAHQAVHFVFLLSPSKGHNHR
                     LKYVSPGLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD"
     gene            complement(8047..9141)
                     /locus_tag="VHI09_000784"
                     /pseudo
     CDS             complement(8047..9141)
                     /locus_tag="VHI09_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
     gene            complement(9150..10550)
                     /locus_tag="VHI09_000785"
     CDS             complement(9150..10550)
                     /locus_tag="VHI09_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ6120675.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSNAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEIIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALDLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            10775..11470
                     /locus_tag="VHI09_000786"
     CDS             10775..11470
                     /locus_tag="VHI09_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEQ6120676.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISAFLFGDQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDSSQYAVVICFATFLINDSYTFYNWL
                     MLQRYQEKKVKKG"
     gene            complement(11475..11633)
                     /locus_tag="VHI09_000787"
     CDS             complement(11475..11633)
                     /locus_tag="VHI09_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120677.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(11702..13015)
                     /gene="obgE"
                     /locus_tag="VHI09_000788"
     CDS             complement(11702..13015)
                     /gene="obgE"
                     /locus_tag="VHI09_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEQ6120678.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVDEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGIGLGTQFLRHIERTRVILHVIDMSANEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDEADLVDEEAYYGFAETEKDFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDLVRIGKFEFEFVD"
     gene            complement(13072..13200)
                     /locus_tag="VHI09_000789"
     CDS             complement(13072..13200)
                     /locus_tag="VHI09_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEQ6120679.1"
                     /translation="MAFGENGPRKKTTFEKVTMVVVILMVLVTVGGLIASALSVLM"
     gene            13444..14786
                     /locus_tag="VHI09_000790"
                     /pseudo
     CDS             13444..14786
                     /locus_tag="VHI09_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(15086..15958)
                     /locus_tag="VHI09_000791"
     CDS             complement(15086..15958)
                     /locus_tag="VHI09_000791"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEQ6120680.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            16182..17492
                     /locus_tag="VHI09_000792"
     CDS             16182..17492
                     /locus_tag="VHI09_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEQ6120681.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNAIYMILYLIAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPVLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISAQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            17570..>17750
                     /locus_tag="VHI09_000793"
     CDS             17570..>17750
                     /locus_tag="VHI09_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ6120682.1"
                     /translation="MPRKTFDKAFKLSAVKLILEEEQPVKMVSSTLKIHPNSLYRWIQ
                     EYEKYGESAFPGHGSA"
BASE COUNT         5414 a         3784 c         3231 g         5321 t
ORIGIN      
        1 cttaatgttc aacattttgt tataattaac catatttttt agttttataa ggagattagt
       61 atgacgcaca aaataaaagt attgctgctt gcgataatgt ctattttttt gacatgcaat
      121 attgcaagtg ctgaaactat tgctattgtt tcagatacag cttatgcccc atttgaattt
      181 aaagactcag atcaaattta caaaggaatt gacgttgata ttattaatga agtagccaaa
      241 cgtcaatctt gggatttcag tatgagtttc ccgggttttg atgcagctgt aaatgctgta
      301 caatctggtc aagcgagtgc tctaatggcc ggtacaacca ttacgaatgc tcgtaagaaa
      361 gtctttcatt tctcagagcc atattacgat accaaaattg tcattgcgac acgtaaagcc
      421 aatgccatca aaaaatacag tgacttaaaa ggaaaaacgg tcggtgttaa aaatggaaca
      481 gcggctcaag cctttttgaa taactataaa aaaaagtatg attatactgt taaaacattt
      541 gacacaggtg atcttatgta taatagttta tctgctggtt ctattgccgc tgttatggat
      601 gatgaggcgg ttatccaata cgcaatcagc caaaaccaag atattgctat taacatgaaa
      661 ggagagccca ttggaagctt tgggtttgct gtcaaaaagg gaagcggata tgattatcta
      721 gttaatgatt tcaatacagc tcttaaagct atgaaagctg atggtaccta ccaagctatc
      781 atgaccaagt ggttaggcac agatgataaa gctaccacca gtcaggcaac gggaaatcca
      841 tctgccaaag ctacacctat aaaggacagt tataaaattg tctctgattc gtcttttgca
      901 ccttttgaat ttcaaaatgg taagggcaaa tacgttggta ttgacataga attaatcaaa
      961 gctattgcta aacaacaagg tttcaaaatt gaaatcgcta atccaggttt cgatgctgcc
     1021 ttaaatgctg tgcaatctag ccaagcagat ggggtcattg ctggtgcaac tattactgac
     1081 gctcgtaaag ctatctttga tttttctgat ccttattata cttctaatat cattttagct
     1141 gttaaagctg gaaaaaacat caagaactat gaagacttag acagaaaaac agtaggtgct
     1201 aaaaacggca cttcatctta ctcttggtta aaagaaaacg ctcctaaata tggttataat
     1261 gtcaaggcat ttgatgatgg ttctagcatg tatgatagct taaattcagg ttctgtagat
     1321 gctatcatgg atgatgaggc ggttcttaaa tacgctatct ctcaaggtcg tcgctttgaa
     1381 acacctcttg agggcatttc tactggtgaa gttggttttg ctgtcaagaa aggaactaat
     1441 ccagaattaa tcgaaatgtt caacaatggc ttagctgctc tcaaaaaatc tggtcagtat
     1501 gatgacatta tagataaata ccttgactct aagaaagctg caactccttc tgaaaaaggt
     1561 gctgatgagt ctactatttc aggcctatta tcaaataact acaaacaact attggcagga
     1621 cttggaacca cgctcagttt aacccttatt tcatttgcta ttgctataat tatcgggatc
     1681 atctttggga tgatggccgt gtcaccaact aaatcacttc gactcatttc aacggccttt
     1741 gtggacgttg ttcgagggat tcctttgatg attgtggctg ccttcatttt ctggggagta
     1801 ccaaacctta tcgagagtat gaccggccac cagtcaccga ttaatgattt cttagctgct
     1861 acaattgcac tgtcacttaa tggcggagcc tatattgctg aaattgttcg cggtggtatc
     1921 gaagctgttc cagcagggca aatggaagct agtcgaagtc ttggtttgtc ttacggaacc
     1981 acgatgagaa aagtaattct cccacaagct gtgaaactaa tgttacctaa ctttatcaac
     2041 cagtttgtta tttcattgaa ggatacaaca atcgtctcag caattggttt agtggaactc
     2101 ttccaaacag gtaaaatcat tattgctaga aattaccagt cgttccgtat gtatgctatt
     2161 ttagcaatta tttaccttat catgattaca ctcttaacaa gacttgcaaa acgtttagaa
     2221 aagaggctta actaatgaca gaattaaaaa ttgatgttca agatttacac aaatcttatg
     2281 gtcaaaatga agtcttaaaa ggaattgatg ccaaattcta cgaaggtgat gtcgtttgta
     2341 tcattggtcc ttctggttct gggaaatcaa cctttctacg taccctcaac cttctagaga
     2401 cgattaccag tggtaaggtc atggtggacg gttttgaatt atcagatcct aagactaata
     2461 ttgacaaggc acgtgaaaat atcgggatgg tattccaaca cttcaacctt ttccctcaca
     2521 tgactgttct tgagaatatt atctttgctc ctgttgaatt aggtaaagag tctaaagaag
     2581 ttgctaaaaa gcatggcatg gcacttctag aaaaagtcgg actttctgat aaggccgacg
     2641 ccttaccagg aagtttatcc ggcgggcaaa aacagcgtgt cgccatcgcc agaagtttag
     2701 caatgaaccc agatattatg ctctttgatg aaccaacttc tgctcttgac cccgaaatgg
     2761 ttggggacgt tttgaacgtt atgaaagact tggcagaaca aggcatgacc atgttaattg
     2821 ttactcacga aatgggattt gctcgccaag tagctaaccg tgttatcttt acagacggtg
     2881 gtcaattctt ggaagatggt actcctgaag agatttttga tcatcctaag caccctcgtc
     2941 tcattgaatt tttagacaag gttttaaacg tctaatagag cagacaccat actaacttcc
     3001 aaaaaataat aaggcaagga aacctatctc cttgcctttt ttggttactg ttattattca
     3061 aaccagcaaa atagggatac tcttatgact ctctccttca taagagtgtc ccctatgctt
     3121 ttttatgggc gtttatgttt agtgaggtaa taacctaatc ccgtaagagc tagtcccgtc
     3181 actatgtgta agtatttgtt accaaccatc ggactaatgg caatgagaaa aaagccaaca
     3241 gccattaaaa aagttactcc atttttttct gtcatgcaag gtcactcgct ttttctttat
     3301 tggccaaaat gacaaatggt gcccagataa ggaaggcaac aaagaggtta aacagtgaaa
     3361 ccaaacctgc cataatatta cctccagtag ctaggtaagc ataaagtcca acaggggtaa
     3421 tccaaggtac tgataagaat atcggtggaa tcaaacctat tgcagtagca aaataagcta
     3481 tgctagcaca aattggtggc acaattagcc atggaatcac aaaggttgga ttcaaaacaa
     3541 ttggcatccc aaatgtaatt ggttcgttga tattgaaaac acccattggt gcggacaatt
     3601 tggcaatggt tttatgctct tctcgttttg agaatatgaa gatagcaata atcaatgcca
     3661 aggttactcc tgaaccaccc atttgagcat aagcatcaaa ggacccgcgt gtccaaaggt
     3721 atggtaaatt agcagcgcta tgcgctgtat tgtaagcagc tgtattttct gttaaagcta
     3781 ccatataaat accatccatc actggggcta agacattatg accatgcaag ccaaagaacc
     3841 agagcagttg aactaagaag gttaacaaga taactgaacc aataccctgt gataagccta
     3901 gcaatggtaa ttggatatag gttgaaatga catcacttag ggaagaacct gttaatgcga
     3961 agatgaggta ggcgaaaatt gccgaagcgt aaatcgcaac tgttcctgga ataatggcag
     4021 caaaggcttt attaaccgca ggtggtacat tatctggtaa ggtaatggta atattacgtt
     4081 tggtcaaagc cgcatacaca aaacttgata aaaagccaat aattaaagct gtgaacaagc
     4141 ctgtggcacc gacatacttt agagctattg ctccccattg actagtttca atacttgaag
     4201 ctccacccgc agtaacgact gaaagcccca tttcttttaa attgctaatt aggttagcag
     4261 aggcattttc aagtggtgtc gaaatggtta aagtctgagg tagggtggca ataaatgagc
     4321 caaaagaaat caatcctccc gccaatggat taatcttatg cataacagct aaattgtaac
     4381 cgaaagcaaa ggcaaaaaag agcgccatga taccaattgt cccaaaataa acatagccat
     4441 tgatgtcgat aacaggttgc atagcttttg caaaccctgt ccatcccata ttgtttggaa
     4501 tatcgcgtaa aaaaacgttc aataagacag caactgaacc tgccatggta ataggcatca
     4561 ttgaaataaa ggaatcacga atagctacta aatgccgttg tgacccaatt tttcctgaaa
     4621 tctccataaa cttatcagaa aaatgcgaca taaatcttct cctcttttta tttttttaat
     4681 gtaacaaagg taaccttaaa agcagcggtg tctgcctaac acaattatct caaaatcatc
     4741 cttgttaaac gtgatgctta gagataaccg gtaatcggct cttatccaaa tgaacccttc
     4801 tctctcattc aaggtctatg aataatcttc acaaagacga tggttgctgc aactgtttag
     4861 catattggat aagggctgct aactgagctt gagattggcg ttctaggctt tttttgaaca
     4921 gtttgccaac aataaaatga tttgcttttt gaaaaatatc tagaggatga tagtcataag
     4981 aataggtaat tctagtccga ttctccccca gagcctctaa tcgataagcc aattccttta
     5041 tcccatgatt ggaactaaaa caagcagtat ataattgcgg agcttctagg gccgtaataa
     5101 gcaccctcac tgtacgctct ttgtatttac cataatgttt aacatagttt atccccgctt
     5161 tgatatcatc tcttgacaac tgtctcaagg tattttgtcg ataatcttct tggagcgata
     5221 tcagcatggc ttcaaaacaa tgatgaatgg aaacatctcc ttctgctgaa ataatcatga
     5281 ctcacctctt tgtattgctg acattttgtc gtaaacggta accaattcct tggctaaatc
     5341 aataaaggct aaggcagtca tcatgtgatc ttgcccatgc accatcaata aagacaattc
     5401 aaccgcatca ccagctgctt cttgggagag caaatgtgtc tgcgatttat gggcaatatt
     5461 taacgagttt gtcgcttctt gaagtaagtg ctgagcctcc tcaaactgcc catctcgtgc
     5521 tgcatgaata gcctctacgg ccttgccctt ggcttcgcct ccatacatga ttaagcccat
     5581 aatagcttct aagttggatg gttccgtcat gcctactcct ccatcatcgc tagggctgtt
     5641 tcaaggactt tttcaccgtt cataagacca taatcagcca tgttaatggc atctacttta
     5701 atatctggtt caaacttatc tttaaaatcc tttagtaaga atttgacttg tggacctaac
     5761 aagagaaagt caatgctatt agcagccact tcgttgtcag cttcactcac aggaactgcc
     5821 caaatagaaa cttcaaggtc acgcgcttgg gctgcttttt gcatcttagt caccaacaca
     5881 ctagttgaca taccggcatt acaaactaac ataatagatt tcataaggtt tctcctttta
     5941 atcgttttaa tcatgaataa gtggggtaaa cactgtcatg aatttggaat aatttggttc
     6001 ttctagtaac gcttgctgaa gctccacctg attgacaaaa gatgccaaac ctggtgatac
     6061 atattttaac ctatgattat gccctttaga cggggataat agaaagacaa aatgaacagc
     6121 ttgatgcgcc ttgtcccact caagaggctc tcggcaaata gccactacta cctgttcaga
     6181 ataagtcatg ggattagcag gatgcggaaa ggcaagcact tctccataga tgactgggct
     6241 gtaattttcc ctcagtacca tttgatgata gaagtcctct acaaacgttt ctgttcccgc
     6301 ctcgtcaaga caagcaatca tccgtaaaag caagtcttcc tttgagattt tctcgtctaa
     6361 ataaagaaaa cgattagggc taaaaacacc tgttagaagc tgctctgctt ttgctaaact
     6421 catctgtgac ggtaaactga cctctttctt gataccttct tgttctccaa taaattgtcg
     6481 aatagtttca atatcctgat ttgacaaaaa aacactgaca gtaatcactg gtgttaaaaa
     6541 catcagattg gcaaggctga tagaggaaat aatcaaatca acggttttca gtcgctcttc
     6601 ggttatttca tagtaactga taacatcgac aatacgcaaa cgtccttcaa attccctttc
     6661 caagcgattt ttcaacatca tggcactacc ataacctgtg gcacaaacta ctagaaccct
     6721 caatttatga cggtttgaat aacgctcaat ggccgccatt aaatgcaaac taatataagc
     6781 ccattcatca tctgagacat cattattttg acatacaagc aaatccgaaa aggtctgctt
     6841 ggtaagggta aatatttcag gatactggct tttaatttcc tcagttaaag gattagtcag
     6901 ctgaatatgg ttttcaagac gtgtggtcaa gggcatcatg tgggctaata aaccttgaaa
     6961 taaattagta tctgtttcta aagtcatgcc tgtcaattga ctaatcttgg caatacatgc
     7021 ttttaaatgg tcctgcaact tttcatcggt attatcatct tgccaatgct taccaacaga
     7081 atgtttgaca tttagatgca gtgcaatata attagcctcc tctttaggaa aacgtatccc
     7141 catcacttct tccacttgat acaaaatgcg cagagctact tgatactcat cagactgacg
     7201 aatagctatt ggaatggaaa acagttctaa tggacacccc gatcgaatac gctgaaccat
     7261 tagggcaata tgcaagacta gattattcat gacaaaatca gaaagtttca acttagcatc
     7321 ccgacattca tccaagacaa taatcatgat ttctgcaaaa ttaatacctt caagaaaggt
     7381 attacctacc atggtggaca aggtattatc aaaactagta acaaaaaaat agtccatgat
     7441 aaaatgccta atgtcctctt ctttacctct tatttgaata tgttggtgag ttatttcaag
     7501 ctctaactgg taaggtatta tgcgctcctt aatgcctgct aaaacatgtt ttaaagttgt
     7561 ccgactgata tacaattctt gacaaagctc ttcaaaatcc tgaacggcat cttcaaaaaa
     7621 gaatttatga agcacataat cttctcgatc aaccgattct tccacttgag tcacatctgc
     7681 caaacgtttt ttagacgtca cactttcttg ccaaaagatg tcaaatgcca tagaatggtc
     7741 aatttctaag cagtaacctc gaccttgttt agaaatgatg tgagctccgt gttcaggaag
     7801 gctactaatc agttctttga ggtatttcct aaccgtacgg tcactcatac caataacctc
     7861 agccagagtt gcactagaga caaactgctc tttgtgatgg attagaaatg ttatgatggc
     7921 cagttctttt tttgataaca ttagtcctcc ttactacttt agaaaacgct ttctaccctc
     7981 ttttagtgta ccaaatattt cctgaaaaag gtgaccatca ttttccacat tgattgtgaa
     8041 aagaatctat gatgggtcaa tctccaaaca aaattcctgc caaggtcgca aatcatctaa
     8101 caaactaaga tagtcctctc tgacctcagc aaccacatta gcttttgaac taatcgtaaa
     8161 atttttcaaa gcaatttgca gttctccctt atatcgatga tagcggtcat tgtcaataat
     8221 aatgctacct cgtttgacct ccttagattg gtctcttgga gcaatctatt cctgagcata
     8281 gactagacgt ggcatggttg atcgaatcac ataatcagat acatcgccac gataacaatg
     8341 cggatagcca atgatttgct cctcaacttc tgtcaaatcg atgataggcc taaccttgat
     8401 cgttgtgact ggtcttgcta aagcctgagt acatgctgcc aattcttctt ccgaaatgaa
     8461 ttggttagat atgaggatat tatcaatggt tcctatcgct ttcattaatt ctacttgcag
     8521 gccaatgggc aagtgcctat ggtcttccac tgtcggcaag ccttctgcca gtagccaagg
     8581 gccttcagat gctgactgag cggtaataaa agcagccgtt tcaatatcat gctcatgata
     8641 aaaacgtgac atatccttaa aatgttgcca tgacaaaccg gtaaattcat gtggataaaa
     8701 attatggcaa ccaataatat tgctccgttc tgcatctgtc gctagcaatg acatcaataa
     8761 ttgtttatca gtgctcatat taagttcaat tttaagccca aagggatttc ttgttaaagt
     8821 tacaatctct gctagaggga gagcctcgtc taagcgtaag cccgctaacc cgaaggcatg
     8881 agctcgttca ataagctgat ctgaccaacc agcttggcta ataaagcttg gactaacgtc
     8941 agcaatcact cgaatgccta attgatttgc ataagcaatg agctctgcgt agcaatgaaa
     9001 catctggtga tcagctggtg ccagctgtaa gagactcata aacaagcgct tggctccata
     9061 gctatgacat aagtcaatat aagctttact cttggtaaca tcataccgct cagggtatag
     9121 agaaaatcct aaatcaacca tattgcctcc tatttatcaa aaccatttga ctcgctcaac
     9181 gccttaaacc agtaacctga tttttttatg gttcgctttt gactctctaa atccaaagct
     9241 accaaaccat atcggttttt ataagcattt aaccaagacc agcaatcaat aaaagtccac
     9301 aagaggtagc ctttgcagtt ggctccttct cccaaagcct tatgaagctg gataagatga
     9361 tcctcgataa aggtgatgcg gtagtcatct tgtatctggc catctgctaa aaaagcttct
     9421 tcaccttcga cacccatacc attttccgtg actagccatt cgatgttacc atagtgttct
     9481 tttaaatcaa gggcaatatc ataaagtcct ggttcataaa tttcccaacc ccggtgagga
     9541 ttgacttttt taccaggcat atcataaggt tcaaaataaa tatctaagat aagggggtcc
     9601 ccctcttgct gtgacttact aggtgcctga acccgcaaag gctgataata attaactccc
     9661 aaaaaatcaa cagtattgtt tttgataatc tctaattcat cagcactata ttgaggtaat
     9721 aaatcagctt cctctaaaat ggaaactaac tctgctgggt acacacccaa aactgatgga
     9781 tctaggaaag atttggtttg gaaaagctct gcgattctag cagccttgac atcttcagga
     9841 gcattactcc gaggataagc aggtgtcatg ttaagcacaa tactaatctt gtggtcggga
     9901 tgaagcttat gacaagcttt gaccgccagt gaactggcca gttgtgtgtt gtaagccact
     9961 tgaactgctg ccttggcatc tactttacaa gggtagtgat aataacctaa atagccacat
    10021 tcgactggaa caatcggctc attaaaggta atccaagtgt ttactaaatc accaaaaagc
    10081 tcaaaacagg tttttgcgta ggtttcataa gcccagacag tagctttgtt ttcccagcca
    10141 cctttttctt gcaaggcata ggggagatca aaatgataga ggttaacaat caatttaata
    10201 ccttgagcaa taatgtcttg aaatacttct cggtaaaatg tcaccgcctt tggattaacc
    10261 tccccgactc cttctggaat caatcgagac cactggattg aggtccgaaa aatcgtgtgg
    10321 cctgtttctt tcaaaagggc aatgtcaccc ttatagtttt cataaaaggt tgaggttttt
    10381 tctggaccaa tttggtgatg aaacttagct gactcaatac taaaccagta atcccagttg
    10441 ctaggtcctt tgccatctcc aggaaccgtt ccttcgcttt gaggcccaga tgtggaactt
    10501 ccccataaaa agccatctgg aaattggtaa cggtgttttc tttgtttcat atcttttgtc
    10561 ctttctttgc tcacttctct ttctattata agaagaaaaa caggtaatgt aaacgcttta
    10621 aaaaatctgt tttttcctta tcatcacgga aaataagtga tttaaagctt gacatatttt
    10681 attatcttaa agagcctatc taacctgccc tgctacttct tatcaaaacc aagcccctat
    10741 tgacagatgc cttagcctat gtttgaatag gggtatggct tatttaaact actttacaaa
    10801 aaccgagtgg gcactttggt taagctctgt cttggccatt ttaattagcg cctttttatt
    10861 tggtgaccaa gctcccttag ctttaatagc ttctttgatt ggtgtgacct ctctcatttt
    10921 tagtgctaaa gctaatccta ttggtcaagg gctcgtcatt attttttcca ttatctatgc
    10981 ctatctttca ttacgaaaca gttactatgg agagttaatg acttatcttt tcatgaccct
    11041 ccctatgact attttttcac tttttacttg gttaaaccat ccttttgaag ggaaaaaatc
    11101 acaagtgact atttcacgat taacacccac tgatcgaaga tgtcttttcg tttttactat
    11161 actggtcact cttattttct acggcatctt ggcttctatt caaacagcct atttacttgt
    11221 ctccacttta tcgattgcca cttctttttc agccgtttat ctcagttata agcgtagccc
    11281 ttattttgct ttagcatacg ggctcaatga tctagtcctc attctcttat ggatgcatgc
    11341 tgctcaaaca gattctagcc aatatgcagt agtcatctgc tttgccacct ttcttattaa
    11401 tgatagttac actttttata attggctaat gctccaacgt taccaagaaa aaaaggtcaa
    11461 aaaaggatga gggcctaagc ctcatccttt ttcaattttt cacgttctaa gcgcaaacgt
    11521 ctattgggat ttaaaagatt aaacaattct tctaatttct cagcattcca aacatctgct
    11581 gccgatacaa aatttccatc cttatcctta aaggatatcg gtttatcacc cataatcaga
    11641 aacctccatc ttaaaggtaa aacacaaggt tgctatggca atgccatttc taaagaccac
    11701 attaatccac aaattcaaat tcaaatttgc caatacgaac aagatccccg tcttttgctc
    11761 cacgttcacg aagggcttcg tcaaccccca taccgcgcaa ttgacgggca aatttcatga
    11821 tggattcgtc acgttccata ttggtcatga caaagagacg ttccaatttc tcaccagaca
    11881 agacccaagt agcatcatca tctcgggtaa tctcaaagtc tttttcagtt tctgcaaagc
    11941 catagtaagc ttcttcatcc accagatcag cttcatcata taataagaat tcgtctgttt
    12001 tagcaagtaa ttctgccgtt gcttctaaca agttctctaa gccttgatgt gctaggcttg
    12061 aaatagggaa aatcattggc aaatcatcaa actcgtcata ttgggcagcc aattttttct
    12121 tgaaggcttt caagttttct tgagcctctg gcatgtccat tttattagcc acaataatct
    12181 gtgggcgctc catcaatcgc aaattatagg tttccagctc gttattaatg gaaacataat
    12241 cttcgtaagg atcacgaccc tcattagcag acatgtcaat cacatgaaga ataacccgtg
    12301 tccgctcgat atgacggagg aattgagttc ccaaaccaat cccttggcta gcaccttcaa
    12361 tcaaacctgg cagatctgcc atagcaaagc tatcacctga ctttgtacga accattccaa
    12421 gattaggaac aatagtggta aaatgatagg caccaatttt cggtttagct gatgacacaa
    12481 cacttaacaa agttgatttc ccaacagatg ggaatccgac caagccaacg tcagctaaga
    12541 ttttgagttc taactctaac tgacgttcct cacctggctc accattttca gcaatctctg
    12601 gcgcaggatt acgaggagtt gcaaaacgaa tattcccacg gccaccacga ccacccttag
    12661 caatgaccac ttcttgacca tgttctacta agtctgtaat tacttttcca gtttcagcat
    12721 cacgaaccgt tgtcccttga ggaacaaaga caatcaaatc ttctgcacca cggccgtgca
    12781 tgcctttggt catacctttt tcaccagatt tcgccttgaa tttacgatta taccggaaat
    12841 ccatcaaggt acgcaaacct tcgtccactc ggaagataac tgagccacct ttaccgccgt
    12901 ctcctcccca aggaccgcca tttgggacgt acttttcacg acgaaaagct accataccat
    12961 cgccaccacg gcctgcttgg acactgattt ttgccgtatc taaaaacata ctcatacttt
    13021 ttttattctc taactttctt ttactttaaa aaaacgcctt tcagcgtttc cttacattaa
    13081 tactgatagg gcactcgcaa taagcccgcc aacagttaca agaaccatta ggataacaac
    13141 gaccattgtc actttttcaa aggttgtttt tttgcgaggt ccattttctc caaatgccac
    13201 gttaaatacc tcatctattg atttttcttc ttatatctta acatgaaaga tcgttttagg
    13261 caaatatcag atgaatattt acgctatgta gactattact gagtattgta tcttgtgaat
    13321 ttcatatgaa tatgatacat tagcatttgc aaaataatgt tacagataaa aacctcctcg
    13381 tctggactga actgcactcc aaaagttaga caaaaaatct aacgattggg gtggtatttt
    13441 tttatgaaat ttaatcaaga aacgaaagtt aagatttatg agttacggca aatgggagag
    13501 tccattaaat ccatatcaaa aaagtttgat atggcagaat ccgatgtcaa atatatgatt
    13561 cgcttgattg atcggtatgg ggtaaccatt gttcaaaaat gtaagaataa ttattattct
    13621 ccagaactga agcaagagat aataaataaa gtcttgattg acggccaatc tcagaaacaa
    13681 acatccttag attatgcttt accaacttct agtatgctct caaggtggat agcgcaatac
    13741 aagaaaaacg gttatactat tcttgaaaaa ccaagaggga gaccgagtaa gatggggcgt
    13801 aaacgtaaga aaaatttgga agagatgaca gaggttgaac gtcttcagaa ggaattagaa
    13861 tatcttagag cggagaatgc tgtgctaaaa aagctgagag aataccgctt gagagacgaa
    13921 gcaaaactca aagagcaaca gaaatcattc aagcattaag gaatcaattt cccttagaga
    13981 tgctacttga aatccttgat ttatcacgct ccacctatta ctatcaagtg aagcgactag
    14041 ctcaaggaga taaggatata gaactaaagc atgtgattcg agagatttat gatgagcata
    14101 aaggtaatta cggttatcgt cgtattcata tggagttgcg taaccgagga tttgtggtca
    14161 atcataagaa agttcaacgt ctcatgaaag tcatgggcct agcagcccgc attcgtcgta
    14221 agcgcaagta ctcttcttac aaaggtgagg ttggtaagaa ggctgataat ctgattaaac
    14281 gtcacttcga aggctctaaa ccttacgaaa aatgttacac tgatgtgacg gagttggcct
    14341 tacctgaaag aaaactcttc ttatcacctg ttcttgacgg ctataacagt gagattattg
    14401 atttcactct gtctcggtct cctaacttga aacaggttca aaccatgctt gagaagactt
    14461 ttccagcgga ttcatacagc ggaactattc ttcatagtga ccaggggtgg caataccaac
    14521 actagtccta ccatgatttt ttagagtcta aaggtattct gccatccatg tcccgcaaag
    14581 ggaatagccc ggataacggc atgatggatt ccttctttgg cattctcaaa tctgaaatgt
    14641 tttatggact cgagacaact tatcaatcac ttgacaagct tgaagaagct attacagatt
    14701 acatttttta ctacaacaac aaacgaatca aagcaaaact aaaaggattt agtcctgtcc
    14761 aatacagaac taaatccttt caataattat ttgtccaact ttttggggtc agtacactcc
    14821 tagttaacga ctttgattga ttttattatg atgaatcgtg atttctgaaa acaaaatttt
    14881 cttcatggat ttggcgtgga acttttacaa taaaaacact aacctctccc tatcgttttt
    14941 tcaggtgaat gcttggaatt ggtataataa gcggacacga atcaggtgaa aacagggaaa
    15001 gtatttgaag aggcgtttaa actatctgac ataaaactcg ttctagagga aagacatcct
    15061 ttaaaaagtt agtttatttt gcaacttaga catcaaggta ggttaaaccc ttcatggaaa
    15121 aatcgagact cttagcacta tgggttaaac taccactgga gacgtaatca atcgctaaac
    15181 cacgaaaacg gctaatagtg gtcatatcaa tatttccaga acattcaatc cgagaacgtc
    15241 ctgcaattag agtaatggcc tgttcaatct gttccaatga catattatcc aacatgataa
    15301 tatcaacacc cgccgccgca gcttcttcgg cagcagcaag gctttccact tccacctcga
    15361 ccattttcac aaaaggggca taggcacgcg cttgagcaat cgccttttga acactaccta
    15421 ctgccgcaat gtgattgtct tttagcatga tagcatctga taaattaaag cgatgattat
    15481 agccaccgcc aactctcacg gcatatttct caaaaagacg taaattagga gtggtttttc
    15541 gagtatcaaa taccttaatg cgatcatcgc ctaaggcttc tacataagca gctgtcatcg
    15601 aagcaatccc tgataaatgt tgtaaaaaat tcaaggcaac gcgttcacat gttaagaggc
    15661 ttctcaccga gcctatgatt tctaaaacca aatcgccact agtcaaacga tccccatcct
    15721 taaattgatg aggattctgg aaggtcacct cggcatcaaa tagggtaaaa accctttgaa
    15781 aaacggttag ccccgctaaa acaccagctt ccttggcaaa aagcgacacc ttggcttggc
    15841 catgatgatc aaaaatggca ttggtactgt aatcttcgga atgaacatct tctcgcaagg
    15901 ctgctttcaa tgtatcatct atttgaaaag gggttaaatc agttgaaatg attgacatca
    15961 ctaagtcctc tatttttaat atcacttgta agcactagag gcaattgcta ttaacttccg
    16021 taggaaatca caaacctgaa tacctcattt taaatcccaa caagtgtcaa gacaattgta
    16081 atttttttgt gtataatagt caagtaccaa taggaaacag gtggtctttt gagcgccaaa
    16141 attgccaacc tattttcata ttctaactaa aggacaattc aatgattcat ttaataataa
    16201 ttagcgccat tgctctagcc attggaattg gttaccgcac caaaatcaat attggcctac
    16261 tggctattgc tttttcttac ctcatcgcaa ccactctcat gggattaagc cccaaagaac
    16321 ttcttcattt ttggccaacc tcactctttt ttaccatttt tagcgtctct ctcttttata
    16381 acgttgcaac aactaacggc actcttgatg ttttggctca acacattctc taccgcacac
    16441 gcacccaccc taacgccatc tacatgattt tatacctgat agcaaccctt ttgtctgctt
    16501 taggtgctgg atttttcact actatggccg tttgctgtcc tctagcgatt accctctgtc
    16561 aaaaagcgga caaacaccct ttgattggag ctcaagccgt caattgggga gcttcaggag
    16621 gggctaattt gataaccagt ggctcaggca ttgtctttca aggcctgttt aagcaaatgg
    16681 gatgggaaga gcaagctttt tcacttggca atcatatctt tatcgtcagc attatctacc
    16741 cactcatcgt cttgctttta ttatcttgtt acatccgcta cagtaaggga agaacaaact
    16801 catccttgac tattgaccaa ccaccagtcc tcagcaaggt ccaaagacaa accaccttgc
    16861 tcatgatcag cagcatggtt ttggtttggc tttttccctt gttgcatctc attttcccaa
    16921 acatcgcatg gatagctact tatcgacaaa catttgatat tggctttgtc tcaatactca
    16981 tggtctgcct cgccttaagg ctcaaattag ggaagcaaga agccattttg gctaaggttc
    17041 cctgggctat aattatcatg ctctgcggca tgagcttact aatgtctcta gcggtcaagt
    17101 caggtttagt cacactgata gggcacctca taacaacaac cattcctcat ttctggttac
    17161 ctctgttctt ttgtgtgata gccggagtga tgtcactctt tagttccacc ctatccgttg
    17221 tagccccaac tctctttccc atcattgcta ccatcagtgc tcaaagtcct cacattgata
    17281 ttcgcttact cacaacagcc actattattg gcgctctatc tactaatatc tctccttttt
    17341 catcagctgg ttccctcatt caactctccc ttccccatat agaggaacgc agtcttgctt
    17401 tcaaaaaaca aattcttctg ggtgtgccca tcagcttaag tttggcactc ttgaccatct
    17461 ggattctcat gctgctagct tccttaagtt agatttattg tctaacattt ggttggactt
    17521 ggtataataa agtagacaca aaattaagtg gaaacgagga aaagtcatta tgccaagaaa
    17581 aacctttgat aaagccttca aactctctgc tgtaaaactc atccttgagg aagagcagcc
    17641 tgtaaaaatg gttagttcaa ctttaaaaat tcatcccaat agtctttatc gatggattca
    17701 agaatatgaa aaatatggag aaagtgcgtt cccaggacat gggagcgcac
//
LOCUS       DAUELZ010000031        17596 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.031, whole genome shotgun sequence.
ACCESSION   DAUELZ010000031 DAUELZ010000000
VERSION     DAUELZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17596
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.031"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            409..1122
                     /gene="deoD"
                     /locus_tag="VHI09_000794"
     CDS             409..1122
                     /gene="deoD"
                     /locus_tag="VHI09_000794"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ6120683.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMVLGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKIGLETLIAND"
     gene            1115..1903
                     /locus_tag="VHI09_000795"
     CDS             1115..1903
                     /locus_tag="VHI09_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEQ6120684.1"
                     /translation="MTNNQTLDILLDVYAYNHVFRIAKALPNIPKTVLYLLEMLKERR
                     ELNLAFLVEHAAENRTIEDQYHCSLWLNQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWHFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRREPFYFDDMNAIIKKELSLWRQSIV"
     gene            complement(1983..2888)
                     /locus_tag="VHI09_000796"
     CDS             complement(1983..2888)
                     /locus_tag="VHI09_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ6120685.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHNKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            3110..3646
                     /locus_tag="VHI09_000797"
     CDS             3110..3646
                     /locus_tag="VHI09_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120686.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDLLYEHIQRVNLEE
                     EAFIKYYQGPKVPTGEFE"
     gene            3923..4615
                     /gene="pyrF"
                     /locus_tag="VHI09_000798"
     CDS             3923..4615
                     /gene="pyrF"
                     /locus_tag="VHI09_000798"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEQ6120687.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRYIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            4673..5302
                     /locus_tag="VHI09_000799"
     CDS             4673..5302
                     /locus_tag="VHI09_000799"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEQ6120688.1"
                     /translation="MTLASQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            5499..6953
                     /locus_tag="VHI09_000800"
     CDS             5499..6953
                     /locus_tag="VHI09_000800"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEQ6120689.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGLPFAGVPIFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVK
                     RLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGIS
                     ALAPASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKDSIYQSLQRPLTIAFYQRLSDGSPVSLDTAKALKQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTLGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            7071..7934
                     /locus_tag="VHI09_000801"
     CDS             7071..7934
                     /locus_tag="VHI09_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ6120690.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWETIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMVKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DVQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPVIDTKDVN"
     gene            7963..8613
                     /locus_tag="VHI09_000802"
     CDS             7963..8613
                     /locus_tag="VHI09_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ6120691.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFFTGLFLGLGLALLGR
                     SRQPLIHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWLIAFLLERYQEFLENKLALV"
     gene            8746..9399
                     /locus_tag="VHI09_000803"
     CDS             8746..9399
                     /locus_tag="VHI09_000803"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEQ6120692.1"
                     /translation="MTHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPQRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNSKHHIIESPHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGVDWLK"
     gene            9531..10799
                     /locus_tag="VHI09_000804"
     CDS             9531..10799
                     /locus_tag="VHI09_000804"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEQ6120693.1"
                     /translation="MILIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVQVVAFAQQLGAKVTAEVSPQHFSTTEDLLLTAGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFV
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(10857..11498)
                     /gene="plsY"
                     /locus_tag="VHI09_000805"
     CDS             complement(10857..11498)
                     /gene="plsY"
                     /locus_tag="VHI09_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEQ6120694.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            11633..13582
                     /gene="parE"
                     /locus_tag="VHI09_000806"
     CDS             11633..13582
                     /gene="parE"
                     /locus_tag="VHI09_000806"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEQ6120695.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGAVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVESIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKTEKIAYAWTDGEL
                     EDLRREFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            13673..16132
                     /gene="parC"
                     /locus_tag="VHI09_000807"
     CDS             13673..16132
                     /gene="parC"
                     /locus_tag="VHI09_000807"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEQ6120696.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKEADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            16210..17277
                     /locus_tag="VHI09_000808"
     CDS             16210..17277
                     /locus_tag="VHI09_000808"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEQ6120697.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAARLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGGIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            17398..>17596
                     /locus_tag="VHI09_000809"
     CDS             17398..>17596
                     /locus_tag="VHI09_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ6120698.1"
                     /translation="MLHKEKPDYNRNQYGFYTLDQLVPDEHFLRQVEAVIDFDFIYDL
                     VEDTYSSDNGRPSLDPVMLVKI"
BASE COUNT         5530 a         3192 c         3680 g         5194 t
ORIGIN      
        1 cttatttgaa ctcgattttt agctctcttc tattcaatat caattatgaa aggaatgggg
       61 ttgtattcaa ttgaatacgg gcggaaagct cggaaactag ataaactaac ttttgagctc
      121 acacatggcg ttagttccct aaatttcagt cgctttctgt ccgccctctg accttctaca
      181 aaataaaagg aaaggtaggg ctaactgtat tcattgaatt gaatacgggc ggagaactgt
      241 ttttcttcct tataaaaaat aaaaaaataa gaaaggtgag ggctgagttc attaatttga
      301 attcgggcta aggacttggt caaaaagata agttgtctag aaaatcagga ttttctgcca
      361 acttcctatt ttgccatcgt tcttttaacg ccctcagtat cataatttat gtctatccat
      421 atttctgcca aaaaaggcga tattgctgat aaaattcttc tacctggaga ccctttgcgc
      481 gcgaaattta tcgctgaaaa tttcctagaa gatgcagtgt gttttaacga agtgcgtaac
      541 atgtttggct acacaggaac ttacaaaggg caccgcgttt ctgtcatggg aactggtatg
      601 ggaatgcctt cgatctctat ctatgctcgt gaattaatcg ttgattacgg tgtcaaaaca
      661 ttgatccgtg ttggtactgc gggtgcgatt gacccagaag ttcatgtgcg tgaattggtg
      721 cttgcccaag cagcagcaac caactcaaat attatccgta atgacttccc agaatttgat
      781 ttcccacaaa ttgctgattt tggtttactt gacaaggctt accatatcgc cagagaaatg
      841 ggagtaacaa ctcacgttgg gaatgtgctt tcttctgatg tcttttacac taacatgcct
      901 gagcgtaata tggttcttgg taaattgggt gttaaagcca tcgaaatgga agcagcagct
      961 ctttactatt tagctgctca gcaccatgtc aaagcccttg gaatcatgac gatctcagat
     1021 aacttgaatg atccaaccga agatactaca gctgaagagc gccaaaccac tttcactgat
     1081 atgatgaaaa ttggtttgga aactttaatt gccaatgact aataatcaaa cgctagacat
     1141 ccttttggat gtctatgctt ataatcacgt ctttagaatt gctaaagcct tgccaaatat
     1201 ccctaaaact gttctctatt tactagagat gttaaaagag cgcagagaat tgaaccttgc
     1261 ctttctggtg gaacatgcag cagagaatcg gaccattgaa gaccagtacc actgttcatt
     1321 atggcttaac caatcgcttg aagatgagca gattgccaat tacattttgg atttagaagt
     1381 taaagtaaaa aacggtgcta ttattgattt cgtcaggtca gtgtcgccta ttctttaccg
     1441 actttttctc agactaatca cgtcagaaat tccaaacttc aaggcttata tttttgatac
     1501 aaagaatgac caatatgata cctggcattt tcaggccatg ttggaatctg atcacgaggt
     1561 ttttaaggct tacctgtctc aaaagcagtc tcgcaatgtg acgaccaaaa gcttagcaga
     1621 catgttgacg ttgacctcct tacctcagga aatcaaggac ttggtttttt tgttacgaca
     1681 ttttgaaaag gctgttcgta atcctctggc tcatttgatt aagccttttg atgaagagga
     1741 attgcatcgc accactcatt tttcttctca ggcttttttg gaaaacatta tcaccttggc
     1801 aactttttct ggtgtaatct accgacgtga gcctttttac tttgatgaca tgaatgccat
     1861 tattaaaaag gagttgagcc tttggagaca atctattgtc tgatatttat caaaaaacac
     1921 gataacttga ccgatacagg tcttactacc gtgttttttt agtatgacga ttgaaaaaga
     1981 aattaatcaa aggttacttc ttcgagaagg tattcgataa aacgttcccc cattttggac
     2041 aagttggctt tttcatgctt gataaaaaca atgtcaattt cgtcagggac atctagcggg
     2101 atagccacaa tctggtcacc atttagattg ctatttagaa taccactggc aacggtgtag
     2161 ccatctaaac caatcattaa gttaaagagg gtggcacggt cactgaccac aattgatttg
     2221 ttgtggggca tctgggacat catctcttct gaaaaataaa aggagttgtg aatcccttgg
     2281 tcatagctca ggtaaggaaa atctctcaag tcgtccatgc ttaataggct tttagttgct
     2341 agaggatttg atttgctgac aaaaatatga ggctgtgcct taaataatgg agaggcagtc
     2401 aaatgattgt catcaaataa tttggtcaag acatctcggt tatagtcgtt gataaagaga
     2461 acaccaattt cagatcgaaa gtttttgaca tcatcaatga tttcccaagt tcttgtctcg
     2521 cgcaaaaaga gttcgtaccg agtcatgtcg gtccgcttga ggagtgagac aaatgcattg
     2581 actacgaagg cataatgttg agatgaaacg ctaaagagct ctcggccagt attgtggttt
     2641 ttatagcggt cttctaacag tgatgtttgc tctataattt ggcgagcata agataaaaat
     2701 tccaccccat cttttgtcaa ggtgatccct ttaggatttc ggttgaaaat ggtaatgccc
     2761 atctccattt ccaaatcctt gaccgcattg gatagacttg gttgtgtgat aaataattgc
     2821 ttagcagctt cattcataga gccgcactca acaattttga taatataatg cagttgttga
     2881 attctcataa gtacagtcta gctcaaaaaa aataaagacg caagcacaaa cgtcatcatg
     2941 ggtttagaac tttgaaaaag cgctggagtc ttttctcaat atggcctagg ataaatatga
     3001 ttcaattttg agagttgaat ctatcaattt cattgccctt ttatttttct tgtgttatat
     3061 taatagtatt aacaaatagg tatttttcta atcaaagggt ggtggtcgtg tgaatgttac
     3121 tgaacaaaat agtcatcaaa tcttaatcca aaaattattg gtcagtatcc actatttgac
     3181 tctttttcga gatgaactga agcttgtaga acgaactcct tctatacttg gaggggaatt
     3241 tccagctcat ttagtacagt ctgagttggg agatattgtt gcggccattg ataccttgga
     3301 tatgcagcaa cgcttgattg agtctacctt ttggtatgaa gaatcagctt ttaagttgat
     3361 gaacaaaact ttggatattg tcgataattg gattaaagga gtggatcatc tgatagattt
     3421 atgccagtcc aaagaagtct ttcaaattat tattggagat aaacgcattc gagtttttgg
     3481 ggtcttaagt gatgtttttt catctttaaa agtgagtgct ttgtcactta aggaagctcc
     3541 tattccggat ttgctctatg aacatatcca gagggttaat cttgaagaag aagcttttat
     3601 aaaatattac caaggcccta aggtacccac aggtgagttt gaataaatga ttgggtaaaa
     3661 atgataaaat gagttgacgt aagtatacaa aaaaagatat agtaggaaaa atgtgttcaa
     3721 ataacaaaaa gttttgacac cttgttttaa aaaagctata atagtttaaa tttagacacc
     3781 tttaagtgag tccagagagg caaacaaggt atacaagata aaaaaaggga attccctttt
     3841 agtttgctgt agcctcgctt ctttggtagc gaggcttttt ggtgcttaat gagatattga
     3901 cacattatag aaaggataag ttatgaaaga agaacgccca attattgctt tagacttttc
     3961 atcatttgag gagactaaag catttttaga cttatttcca gcagaagaaa agctatacgt
     4021 gaaaattggt atggaattat attatgcgca aggaccagat attgttcggt atatcaaatc
     4081 gttagggcat aatgtatttt tggatttaaa attacatgat attccaaata ctgttcgtgc
     4141 tgctatggca gttcttaaag aattagatat tgatatggca actgttcatg cagctggtgg
     4201 tgtagagatg ctaaaagcag ctcgagaagg actaggtcaa ggccctacct taattgcggt
     4261 aacgcagcta acttcaacca gtgaagacca aatgcgtggt gatcaaaata ttcaaacgag
     4321 tcttttagag tctgttttgc attacagcaa gggggcagct aaagctcagc tagatggggt
     4381 ggtttgttcg gcacaagaag tagaagctat caaggcggtg acacctacag gatttacttg
     4441 cttaactcct ggcattcgtc ctaaaggcag taatattggc gatcaaaaac gagtgatgac
     4501 tccaaaccaa gcaagacgca ttggaagtga ttatattgtg gtagggcgac caattaccca
     4561 ggcaaaagac ccagtggctg cttaccaagc cataaaagct gagtgggcag gctaacagat
     4621 aagattagga aaccaacact tgtctgttaa agaagaaaaa ggagaataag aaatgacact
     4681 agcaagccaa attgcaactc aattacttga tattaaagct gtttatttaa agcctgaaga
     4741 tccttttacg tgggcatctg gcattaaatc acctatttac actgataatc gtgtcaccct
     4801 ttcttatcct aagactcgtg atttaattga gaatgggttt gtggaaacta tcagggctca
     4861 tttcccagaa gttgaggtca tcgctggtac agctacagca ggtattcctc acggtgccat
     4921 tatagcagat aaaatgactc ttccttttgc ttatattcgc agtaagccaa aagatcatgg
     4981 agcaggcaac cagattgagg ggcgtgtgct taaaggccaa aaaatggtta tcattgaaga
     5041 tttgatttca acaggtggtt cggtactaga tgcagccgca gcagccagtc gtgagggagc
     5101 tgacgttctg ggagttgttg cgattttcac ttatgagtta ccaaaagcta gtcagaattt
     5161 taaagaagct ggtatcaagt tgattacgct ttctaattat actgagctaa ttgcagttgc
     5221 aaagttgcaa ggctatatta ctaatgatgg tttacattta cttaaaaaat ttaaagaaga
     5281 ccaggtaaac tggcaacaat aaggaactat cagccaggtt tatttttaat gaaaaaatct
     5341 ctttaacccc aattcaaaat gttatgtttt gattgttttg tctacattcc ttacacatca
     5401 cattctaatt tctaaccttt taatggtggt tttcttttat ttgtgtttta ataaaaaaga
     5461 atgttttagg attcatatct tagaaaggaa gagtagctat gacttatcaa gatgcgacgg
     5521 ctatggctat tgctgttcag actggtcaga ccacaccact agaattagta acccaggcta
     5581 tttataaggc taaaaagttg aaccctacct taaatgctat tacctcagaa cgctttgaag
     5641 cagccctaga agaagccaaa caaagggatt tttcaggtct gccttttgca ggagttccca
     5701 tttttttgaa agatttggga caagaattaa aaggtcactc atcaacctca ggttccagat
     5761 tgtttaaaga gtatcaagcg actaaaacgg atttatttgt gaaaagatta gaagctttag
     5821 gctttattat tttagggcgc tccaacacgc ctgaatttgg ctttaaaaat attagcgata
     5881 gcagtttgca cggaccagtg aatttaccaa aggataatac acgaaatgct ggtggctcgt
     5941 caggtggtgc ggcagctcta gtgtcatcag gaattagtgc actagcacca gctagtgatg
     6001 gtggtggttc tattcgaatt ccagcctcgt ttaatggctt aattgggctc aaacctagcc
     6061 gtgggcgcat gcctgtcgga cctggttctt atcgaagctg gcaaggagcc tcagttcatt
     6121 ttgccttgac caaatccgtt agagatacta gaaatctact ctactattta cagatggaac
     6181 aaatggaaag ccccttcccc ctagccacct taacaaaaga tagtatttat caatcgctac
     6241 aacgtccctt gacaattgct ttttatcaac ggttaagtga cggtagccca gtatctttag
     6301 atactgcaaa agctttaaaa caagcagtaa cttggctaag agaacaaggg catcagctag
     6361 ttgaattgga agagtttcct gtaaatatga cagaagtgat tcgtcattat tatattatga
     6421 atagtgtgga aacggctgct atgtttgctg atattgaaga cactttagga cgtccgatga
     6481 ccaaagacga tatggaaacc atgacctggg ctatttacca atcaggtaaa gacatcccag
     6541 cttggcgtta ctcacaggtc cttcaaaaat gggatacgta cagtgcaacc atggctagtt
     6601 ttcatgagac ttatgacctg ttgttgacgt tcacaaccaa cacaccagct cctaagcacg
     6661 gagagcttgt cccagattcg aaactcatgg ctaacttggc tcaagctgaa attttcagca
     6721 gtgaagaaca gttcaatttg gtcgaaacca tgtttggtaa gagtctggct attaatcctt
     6781 acacagctct gcctaatcta actggccaac cagctattag tttacctact tacgaaacta
     6841 aggaaggtct cccaatgggc attcaactaa ttgcagccaa aggtagagag gacctattat
     6901 taggaattgc agagcagttt gaggcagcag gattattgaa gattcctcaa taataatctt
     6961 ataaaacagt ttaactatta tagattaact ttttaaagag attgagcgct cactgataac
     7021 acaacttaat caaaatgtta tactattctt atcttaataa aggagttact atgaagaaat
     7081 cagttacttt actgtctatc ggacttgcca gccttttatt ggcagcatgt gcgccacata
     7141 aatctcaaaa atcaagttgg gagactatca aagaaaaagg ggtgttaaaa gtagctaccc
     7201 caggaaccta tcagccaact tctttttaca acgataataa tgagttagta ggttacgaag
     7261 tagatatggt caaggaaatc ggtaaacgac ttaacattaa agtcaagttt gttgaaacag
     7321 gatttgacca agcctttacc tcagttgata gtggtcgagt ggatatttct ttgaacaact
     7381 ttgacattac cccaaaacgt cagaaaaaat acaatatctc tacgccttat aaatacgggg
     7441 taggaggcat gattgtccgt gctgatggca gctcaaacat ggttaaaaaa gatcttagcg
     7501 actggaaagg aaaaaaagca gctggtgctt ctggaacaga atacatgaaa gtagcgcaaa
     7561 aacagggagc tgaactggtg acctacgata atgttacagg agatgtttat ctaaacgatg
     7621 tggctaacgg tcgaacagat ttcattccaa atgattaccc agctcaaaaa ttatttgtgg
     7681 attacatgtt atctcaaaat ccaaatctaa atgtcaaaat gagcgatgtt cagtacaacc
     7741 caaccgaaca aggaattgtt atgaacaaaa aagacgatag cctcaagaaa aaaatagatg
     7801 ctgttattaa agacatgata aaagatggta gcttgaagaa aatctctgaa acctactatg
     7861 ctggtcaaga tttgacaaaa ccttttggca aagacaaaaa aattcccgtc attgatacga
     7921 aagacgttaa ctaatacttg atgttaaagg agtcctaacc acatgattaa tatcccatta
     7981 atgaaagata gtttaggatt tgtgttatcg ggattacctt atacccttgg tatttcgttg
     8041 ctcagttttt ttacgggtct gtttttaggt ttaggcctag cccttctggg aaggtcacgt
     8101 cagccgttga ttcattatct tgttagagcg tacatctcta ttatgcgagg tgtgccgatg
     8161 atcgtggtgc tctttgttct ttatttcggg ttgccttatt atggtttaga attgccagct
     8221 ttactttgcg cttatcttgg tttttctatg gttagcgctg cctatatttc tgaagttttt
     8281 cgctcttcta tagaagctat tgacaaggga cagtgggaag cagctaaggc tttagggttg
     8341 ccatatgctc ttatggttaa gaaaattatt cttcctcaag cctttcgaat tgcagttcct
     8401 cctctaggca atgtcattat tgatatggtt aaaagttcgt cgctagctgc catgattacc
     8461 gtaccagata tttttcaaaa tgccaaaatt atcggtggta gagagtggga ttacatgtcc
     8521 atgtatattt tagtggcctt tatttattgg cttattgctt ttttgttaga acgttaccaa
     8581 gaattcttag aaaataagct agcacttgtt taatatcctg taaaaaacga atattaaccc
     8641 tggaaatatc atattttcag ggtctttcta tttaaaatgt ggtaaactat atagaaaagg
     8701 acatcgtatt taacttagaa actgacgtga ttagaaagga atcttatgac tcattcaatt
     8761 tggcatgaga aaatcaaatc atttttgccg gagcattact atggtcgcat caatcacttt
     8821 ttagatgaag cctatgcttc cggtcttgtc tacccccaac gagaaaatgt ctttaaagcc
     8881 ttacaagtga ctcctttgga agaaaccaaa gtgttgattt taggacaaga cccttatcat
     8941 ggtcccaaac aggcccaggg cttatcattt tcagttccag aagagatttc tgctccgcca
     9001 tcccttatta atattttaaa agaattagca gatgacattg gtcctcgtga ccatcatgat
     9061 ttaagcactt gggctagtca aggggttctg cttttgaatg cttgcttaac agtgccagca
     9121 ggccaggcta atgggcatgc aggcttaata tgggagccat ttacagacgc tgttattaaa
     9181 gtgctgaatg aaaaagacag ccctgtagtt ttcattttgt ggggtgctta tgcaaggaag
     9241 aagaaagcct tcattactaa ttcaaagcac cacatcatcg agagccctca tccaagccct
     9301 ttgtcatctt atcgtggctt ttttggcagc aaaccctttt caagaaccaa cgctatttta
     9361 gaaaaagagg gcatgactgg cgtagattgg ctaaaataag ccagccattt caactaaata
     9421 gcttaagcta gacattttga tacctttaat aagtgaaatg agcgcagtag atttttccta
     9481 ttaaagaacg taaggtctta tggttaaaac agataaataa aggagaaaac atgatactca
     9541 tcaaaaatgg acgcgttatg gacccgaaat cgcagagaga tcaagtggca gatgtcttga
     9601 ttgatggtaa acagattgta aagatagctt cagctattga atgccaagaa gctcaagtga
     9661 ttgatgctag tggtcttatt gttgccccgg gtttggtcga tattcatgtg cattttagag
     9721 aacccggtca aacgcacaaa gaagatattc acacgggagc cttggcagca gcagcaggtg
     9781 gtgtgactac ggttgtcatg atggctaata ccaatcctgt catttcagat gtcgaaactt
     9841 tacaggaagt gctagctagc gcagctaaag aaaagattca tatttacacc aatgccagtg
     9901 tcacccaagc ctttaatgga aaagacgtga ctgattttaa agctttgttg gaagcaggag
     9961 cagtaagctt ttcagatgat ggtattccct tagaaagttc taaagtttta aaagaagcct
    10021 ttgatttggc taatgccaac caaactttca tttctttgca tgaggaagat cctcagttaa
    10081 atggggttct tggttttaat gaaggtattg ctgaggagca ttttcatttt tgtggggcaa
    10141 ctggtgtagc agaatatagt atgattgcaa gagatgtcat gattgcttat gacaggcaag
    10201 ctcatgttca tatccaacat ttatctaaag cagaatcggt tcaagtagtg gcttttgctc
    10261 agcagttggg tgctaaggta actgctgaag tgtccccaca acatttttca acaactgaag
    10321 atctcttatt gaccgcaggt accagcgcca agatgaatcc tccccttcgt acacaaagag
    10381 atcgcttagc tgtcatagaa ggcttgaaat caggtgttat cacagtcatt gccactgatc
    10441 atgctcctca tcacaaggat gaaaaagctg ttgatgatat gactaaagcc ccatctggta
    10501 tgacaggact tgaaacttct ttgtcattag gtttgacgca tttggttgaa ccaggtcatc
    10561 ttacacttat gtcactatta gaaaaaatga cgcttaatcc agctttgctt tatggttttg
    10621 atgcgggtta tttggcagaa aatggtccag cagatttggt tatttttgca gacaagcaag
    10681 aacgtctgat cactgagaac tttgtttcta aagctagtaa ttccccattt attggtaaca
    10741 aactcaaagg tgtcgtgaaa tacactattg cagacggaga ggttgtttac cctaattaac
    10801 aaatgagtta aaacaaaaaa gcctgacttt cagagaaagt caggctttta gtaaatttat
    10861 tttttaggca cttgtttgct taaatttagc ccccaaggta ttagattttc agtatgatgt
    10921 ttgatacgac taatgttatc tttgtgtctt ataataataa tgaacgcaag taatatcaca
    10981 ataaaagtta aaaagtagtc ataatttggt aaaagaaaat gaatggcagg aaatgttaaa
    11041 acagataaca caccaacgat agctgaaacc acactagcta aagatatcat actaaataaa
    11101 tagagaacta aaacaaagat agatgctaaa aaaaagagat ataacggagc aaagcctagc
    11161 aatacaccag cacttgttgc tacggcctta ccacctttaa agttggcaaa aataggaaaa
    11221 gtatgcccta aaactgcgaa aaagccgata gcaatggatg aaattgaagt cataccaaaa
    11281 ataattggta acaatattga aagtgtccct ttaaacatat caatagctaa ggtagctgtt
    11341 cctgccttga cacctaaaat ccgaaaagta tttgtggttc cagtatttcc tgatccatgc
    11401 tctcgtaagt tgatgtggta aaagtactgt ccaatccata gtccagttgg aatagaacca
    11461 agtaaatagg caatggtaat aaaaagtagt aatttcataa tccttattat aacatattca
    11521 tagattggag aactagatct ggaaaagaat tcggaaaaaa ttagcaaatt attttgcaaa
    11581 attggtaaaa aacttgtaag ataaaaaaga tgaaactgtc tggaggtcgg tgttgactaa
    11641 gaaagaaatt acaattaata attataatga cgatgctatc caagtcctag aagggttaga
    11701 tgctgttcgc aagcgtccag ggatgtatat cggttcaaca gatgctacgg gattgcacca
    11761 cctgatttgg gaaattgttg ataatgctgt cgatgaggcc ttgtctggtt ttggagatga
    11821 tattaaggtt gttatcaata aagatggctc ggtcagtgtg gcagatagcg gacgtggaat
    11881 gccaacaggt cagcatgcca tggggattcc caccgttcaa gttattttta cgattcttca
    11941 tgcaggtggt aaatttggtc agggtggcta caaaacgtca ggtggtttgc acggtgttgg
    12001 ctcctctgtt gtcaatgctt tgtctgcctg gttagaagtc gaaatcacaa gagacggggc
    12061 agtgtatcgt cagcgctttg aaaatggggg gaaacctgtt acaaccctta aaaaagttgg
    12121 cacagctcct aagtctaaat caggaacggt tgtaaccttt atgcctgatg ataagatttt
    12181 ttcaactatt gactttaagt tcaataccat ttcagaacga ttgaaagaat ctgcttttct
    12241 tttgaaaaat gtcaagatga gcttgacaga tcttcgtgga gatgacccga ttattgaaga
    12301 gtttcattat gaaaatgggg ttcaagattt cgtcgaatac ctcaatgaag ataaggaaac
    12361 cttgacacct gttatttaca tggaaggtca agaccaagac tttcaagtcg aggttgcttt
    12421 gcagtataac gatggttttt ccgacaatat cttatccttt gtaaacaatg tgcgcaccaa
    12481 agatggtgga agccatgaaa cgggactaaa gtcagctatt accaaagcga tgaatgacta
    12541 tgctcgtaaa accaatctct taaaagaaaa ggataaaaat ctagagggct cagattatcg
    12601 agaaggactc tcagcagtat tatctatctt agttcctgag cagcacttgc aatttgaagg
    12661 acagaccaaa gacaaattgg gaagtccctt agcgagacct attgtagaga gcattgtttc
    12721 tgaaaaatta accttctttt tactcgaaaa tggagaagta gcttctcatc tagttcgcaa
    12781 agccattaaa gctagagatg ctcgcgaagc tgctcgaaaa gcgcgtgatg actcacgtaa
    12841 tggtaagaaa aacaaaaaag acaaaggctt gttgtcggga aaattaaccc cagctcagtc
    12901 caaaaatgcc aagaaaaatg aattgtatct ggtcgaagga gattctgcgg gaggttcagc
    12961 caaacaagga cgagaccgca aatttcaggc tattttacca ttacgtggca aagttttaaa
    13021 taccgaaaaa gctaaaatgg ctgatatctt gaaaaatgaa gaaattaaca ctatggttta
    13081 taccattgga gctggagtgg gagctgactt taacctcgag gatatcaact atgataagat
    13141 tattatcatg accgatgctg ataccgatgg ggctcatatt cagactcttt tgctgacttt
    13201 cttttaccgt tacatgcgac cattggtaga agccggtcat gtttacattg cccttccacc
    13261 tctttacaaa atgtcaaaag gcaaaggcaa aactgaaaaa attgcttatg cttggactga
    13321 tggtgaatta gaagatctgc gcagagaatt tggcaagggg gctattcttc aacgctataa
    13381 aggactgggt gaaatgaatg ctaaccaatt atgggaaacc actatggacc ctgaaaccag
    13441 gacccttatt cgtgttacta ttgacgactt ggcaagagca gagcgacgtg tttctgtgtt
    13501 gatgggtgac aaagcagccc cacgccgtca gtggatcgag gataacgtca agtttacctt
    13561 agaagaaaat acggtgtttt agtcatacaa gtttctcaca gataattaca agtgctaaag
    13621 acgattgttt tttaggtaat taataaataa tgaaaaagaa agctatttga atatgtcaaa
    13681 cattcaaaac atgtcccttg aggatattat gggagaacgc ttcggccgtt attccaagta
    13741 tattattcag gaacgggcgc ttccagatat tcgagatgga ttgaaacccg ttcagcgtcg
    13801 gattctttat tcgatgaata aggatggcaa tacttttgaa aaaggttacc gtaaatcagc
    13861 caaatcagtt ggtaatatta tggggaattt ccacccgcat ggggattcct ctatttatga
    13921 tgccatggtt cggatgagtc aagattggaa gaaccgtgaa atccttgttg aaatgcacgg
    13981 aaataatggt tcaatggatg gcgatccgcc tgcggctatg cgttatacag aagctcgctt
    14041 gtctgaaatt gcaggttatc ttcttcaaga cattgagaaa aatacggtct cttttgcgtg
    14101 gaactttgat gacacagaaa aagaacccac cgttttacca gcagcttttc ctaacttact
    14161 tgtcaatggg tcttctggta tttccgcagg ctatgcgaca gatattccac cgcacaattt
    14221 gtcagaagtg attgatgctg ttgtgtatat gattgatcat cccaaagcta gtttagagaa
    14281 attgatggaa tttttgcctg ggccagattt cccaacagga ggtattatcc aaggagctga
    14341 cgaaatcaaa aaagcctacg aaacaggcaa gggacgtgtc gttgttcgct ctaggacaga
    14401 aatcgaggaa cttaaaggtg gtaaacaaca aattattgtc actgagattc cttatgaagt
    14461 taacaaagca gtcttggtca aaaagattga cgatgtgcgt gttaacaata aagtcccagg
    14521 catcgtagaa gtacgcgatg aatctgatcg cacaggtctt cgcatagcta ttgagttgaa
    14581 aaaagaggct gatagccaaa ccattctaaa ttatttgttg aaatacacag atcttcaagt
    14641 taattataat tttaatatgg ttgctattga tcactttacg ccacgccaag taggtcttca
    14701 aaaaatctta tcaagctata tcagccaccg caaagatatt attattgagc ggtctaaatt
    14761 cgataaagcc aaagctgaaa aacgcctcca cattgttgaa ggattgattc gtgtcttgtc
    14821 catcttagat gagattattg ctttgattag atcgtctgac aataaggctg atgctaaaga
    14881 aaatctgaaa gtcagctatg atttctctga ggaacaagca gaagcgattg taaccttgca
    14941 gctttatcgt ttaactaata ccgatattgt tactctccaa aatgaagaaa atgatttaag
    15001 agacttaatc acgaccctgt ccgctattat cggtgacgaa gcaaccatgt ataacgtcat
    15061 gaaacgtgaa ttaagagaag tgaaaaagaa attcgccaat cctcgtttat cagagttaca
    15121 agcagaaagt caaatcattg agattgacac tgccagcttg attgcagagg aagaaacttt
    15181 tgttagtgtg acacggggcg gatacctcaa aagaaccagc ccacgatcct tcaatgcgtc
    15241 tagtttggaa gaagttggca agagagatga tgacgaactc atcttcgtta aacaggccaa
    15301 aacaacagaa caccttttgt tgtttacaac tttaggcaat gtcatttacc ggccaatcca
    15361 tgaattgacc gatttacgtt ggaaggatat cggtgaacat ttatcacaaa ctatttcaaa
    15421 cttcgcgaca gaagaagaga ttctctacgc tgatattgtg acttcctttg accagggctt
    15481 gtatgtggct gtcacacaaa atggttttat caaacgcttt gatcgtaaag aactaagtcc
    15541 ttggcgaact tataaatcta aatcaaccaa atatgtgaag ctaaaggatg acaaagaccg
    15601 cgtggttacc ttgtcaccag ttattatgga agacctccta ttagtgacta aaaatggtta
    15661 tgctttgcga ttctccagtc aggaagtgcc tattcaaggg ttgaaatctg ctggtgttaa
    15721 aggcattaac ttgaaaaatg atgacagcct tgcctcagcc tttgctgtga caagtaactc
    15781 tttctttgtc ttaactcaga ggggttctct caaacggatg gctgtcgatg atattcctca
    15841 gacaagtcgt gccaatcgtg gactattggt cctacgagag ttaaaaacca aacctcatag
    15901 agtttttctg gccggaggcg ttcaatcaga tacttcagct gagcagtttg atttgttcac
    15961 tgatatacct gaagaagaaa ctaatcaaca aatgcttgaa gtcattagta agactggtca
    16021 aacctacgag attgctttag aaacgctttc gttatcggaa agaaccagca atggctcctt
    16081 tatttcagac actatttcgg atcaagaagt actggtagct agaacacgtt aaaaagagtg
    16141 aaaactcttt ttaattttat ggtaaattta gaatattaat gcaaaagaat agaataaact
    16201 tgaaaattat tgaaaaaaag ttacaataga aaaaagcaga aaagaggtac gatgatgaca
    16261 atagcaattg attgggacaa cctaggattt gaatatcata agttgccgtt tcgttacatt
    16321 tcttattaca aaaatggtca atgggataaa ggtcaattaa cagaagatgc caccttacat
    16381 atctcagaaa gcgctccagc gcttcattat ggacaacaag cttttgaagg tttaaaggct
    16441 tatcgcacca aagatggctc tatccaacta tttagaccag accgtaatgc agcgcgttta
    16501 caagccacag cagaccgctt gttgatgcca caagtatcaa cagagcaatt tattgatgca
    16561 gctaagcaag tggttaaagc caatgaagac tttgtaccac cttacggaac aggtgcaacg
    16621 ctttacctga gaccgttatt aattggagtt ggaggtatta ttggggttaa accagctgaa
    16681 gaatatattt tcaccatttt tgccatgcca gtaggaaact atttcaaagg tggacttgct
    16741 ccaactaatt ttattgtttc tgaagcgttt gaccgcgccg ctccttacgg aacaggtgct
    16801 gctaaggtgg gaggaaacta tgcaggaagt ttgctaccag gtaaggccgc taaatcagca
    16861 ggcttttctg atgtgattta cttagatcca gcaacccata ctaaaattga agaagtcgga
    16921 gctgcaaact tttttggcat tactgcaaat aatgaatttg ttacgccact tagtccatcc
    16981 atcttgccat ctattacgaa gtattcactc ttacaactag ctgaagaaag acttggtatg
    17041 acagtaattg agggtgatgt tccaattaat gaattggata aatttgtgga agcaggagct
    17101 tgtggaacag cagctgttat ctctccaatt ggcggtatcc agtacaagga taacttacat
    17161 gtcttttaca gtgaaacaga agttggtcct gtgacacgcc gtttatacga tgagttggtc
    17221 ggcattcaat ttggggatat tgaagcacca gaaggttgga ttgtaaaagt agattaaaat
    17281 agagcttgct cttgcaagct ttcagactga agacaaagtc cttagaatca cttttctagg
    17341 ggcttttcat gatatttagg agtataatat aagaaaaagg acttgtgagg ttctcctatg
    17401 ttacacaaag aaaaacctga ttataaccgc aatcagtacg gtttctatac ccttgaccag
    17461 ttagtgcctg atgaacattt cctgcgccaa gtggaagctg tgattgactt cgacttcatc
    17521 tatgacctag tagaagacac ctattcgtct gataatggtc gccctagcct tgatcctgtc
    17581 atgttggtta aaatcc
//
LOCUS       DAUELZ010000032        17108 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.032, whole genome shotgun sequence.
ACCESSION   DAUELZ010000032 DAUELZ010000000
VERSION     DAUELZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17108
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.032"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(33..1199)
                     /locus_tag="VHI09_000810"
     CDS             complement(33..1199)
                     /locus_tag="VHI09_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEQ6120699.1"
                     /translation="MKNQLGQLALEQAKTFGGKLEVQPKVDITNKHDLSIAYTPGVAG
                     VSSAIAKDKTLAYDLTTKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLKKSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDK
                     FGIINEQEAAQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPDDALSTTNIIPDAFKEGVAEIVAKSVRSVVLKS"
     gene            complement(1230..2561)
                     /locus_tag="VHI09_000811"
     CDS             complement(1230..2561)
                     /locus_tag="VHI09_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEQ6120700.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLPVYLVLATLILVTG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHPSYSGQGQLVKIGHSEDMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTGFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKEVVATLSWQYFIVVISVVLTVVSVGFFVSRFLNMNPVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            2722..4263
                     /locus_tag="VHI09_000812"
     CDS             2722..4263
                     /locus_tag="VHI09_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ6120701.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPN
                     PKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLLLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKAGILQLCNQAGQKLIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQ
                     LFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSATKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGGQH
                     ERFNH"
     gene            4244..4909
                     /locus_tag="VHI09_000813"
     CDS             4244..4909
                     /locus_tag="VHI09_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ6120702.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRAQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIMENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(4964..6037)
                     /locus_tag="VHI09_000814"
     CDS             complement(4964..6037)
                     /locus_tag="VHI09_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ6120703.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(6030..6806)
                     /locus_tag="VHI09_000815"
     CDS             complement(6030..6806)
                     /locus_tag="VHI09_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6120704.1"
                     /translation="MKKFANLYLASVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            complement(6803..7597)
                     /locus_tag="VHI09_000816"
     CDS             complement(6803..7597)
                     /locus_tag="VHI09_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6120705.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            complement(7581..8735)
                     /locus_tag="VHI09_000817"
     CDS             complement(7581..8735)
                     /locus_tag="VHI09_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120706.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            complement(8781..9668)
                     /gene="murB"
                     /locus_tag="VHI09_000818"
     CDS             complement(8781..9668)
                     /gene="murB"
                     /locus_tag="VHI09_000818"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEQ6120707.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTSSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVADGTAKDYEDLIAYVIETV
                     ENHSGVRLEPEVRIIGENL"
     gene            complement(9818..10318)
                     /gene="folK"
                     /locus_tag="VHI09_000819"
     CDS             complement(9818..10318)
                     /gene="folK"
                     /locus_tag="VHI09_000819"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEQ6120708.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            complement(10315..10674)
                     /gene="folB"
                     /locus_tag="VHI09_000820"
     CDS             complement(10315..10674)
                     /gene="folB"
                     /locus_tag="VHI09_000820"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEQ6120709.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGGKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            complement(10690..11481)
                     /gene="folP"
                     /locus_tag="VHI09_000821"
     CDS             complement(10690..11481)
                     /gene="folP"
                     /locus_tag="VHI09_000821"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEQ6120710.1"
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCQFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWIDPGFGFAKSVQQNMELLKGLDRVCQLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLS"
     gene            complement(11490..12074)
                     /gene="folE"
                     /locus_tag="VHI09_000822"
     CDS             complement(11490..12074)
                     /gene="folE"
                     /locus_tag="VHI09_000822"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEQ6120711.1"
                     /translation="MKRERLMSIDKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            complement(12103..13380)
                     /locus_tag="VHI09_000823"
     CDS             complement(12103..13380)
                     /locus_tag="VHI09_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ6120712.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVRETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYP
                     RLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRRYWKKHIEKSVLLTH"
     gene            13712..14674
                     /locus_tag="VHI09_000824"
     CDS             13712..14674
                     /locus_tag="VHI09_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ6120713.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMSPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEMGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            complement(14791..16026)
                     /locus_tag="VHI09_000825"
     CDS             complement(14791..16026)
                     /locus_tag="VHI09_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ6120714.1"
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVKELLVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(16184..17108)
                     /locus_tag="VHI09_000826"
                     /pseudo
     CDS             complement(16184..17108)
                     /locus_tag="VHI09_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106750.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         5386 a         3697 c         2946 g         5079 t
ORIGIN      
        1 taaagtataa ctttctcttt ttttcatata ttctatgatt taaggactac cgacctgacg
       61 cttttagcga caatctcagc gactccttct ttaaaggcat ctggaataat attagtagtg
      121 ctcaaagcgt catctgggac aagactggca ataccttttg cggcagcaat ttgcatctca
      181 acagtaatgg tttttgcccg tgcatctaat gctcccctaa aaatacctgg aaaggcaagg
      241 acattattaa tctggttagg aaaatcactg cggccagtac ctacaatgta agcccccgct
      301 tcaagggctt catcagggta aatctctgga atagggttgg ccatggcaaa aattactggg
      361 cgcgctgcca ttttactaat ccactctgct tttaagactc ctggtgctga tacgccaatg
      421 aagatatctg ctccttcaag agcatcttct aaggtacctg atttaaactc acgattagtg
      481 actttcgcta tatccaaatg atgtggcgct aattgagcgg cttcttgttc attgataatg
      541 ccaaatttat ccactactgt cactttcgta gcacctgctg ctaataattt acgggtaatg
      601 gataatccag cagatccacc accgttcacc acaatggata cctcatcaag ggatttttta
      661 agaagtttca aactattgaa gatggctgct aacaccacaa tagctgttcc atgctgatcg
      721 tcatggaaaa caggaatatg acattcctta atcaagcgct gctcaatttc aaaacagcgg
      781 ggcgcactaa tatcctctaa attaatacca ccaaaggttg gtgcaagcgc cttgacaata
      841 ctaataattt cttcagtatc tttggtatct aatacaatcg gaatagcatc gacacctgca
      901 aatgctttaa aaagggctgc ttttccttcc ataactggca tggctgcttc agggccaata
      961 tcccccagac caagaaccgc agtaccatca ctgataacgg ctactgtatt tttcttggtg
     1021 gttaagtcat atgctaatgt tttatctttt gcgatggcag atgacacacc agctacacct
     1081 ggtgtatagg caatgctcaa atcatgttta tttgtaatgt caacttttgg ttgaacttcc
     1141 aatttacctc cgaacgtttt agcttgttca agggcaagtt gacctaattg atttttcata
     1201 ggaaatacct ctctttttat tggtttggat taactaaaaa taattcttaa aatagctgtc
     1261 atggtgataa cggtaatggc cccgcctaga cgagtagcga cttgggcaaa cggcattaga
     1321 ttcatgcggt cagccgtact caaaatagcc acgtcccctg ttccacccat accactttga
     1381 caagctgaaa taatagctgc ttccactggg ttcatgttaa ggaaacgaga gacaaaaaaa
     1441 ccgacactga caaccgttaa caccacacta atcaccacaa taaaatattg ccagctaagg
     1501 gttgccacaa cctcttttaa ggggatataa agcagaccaa gccctgccat taacggaaaa
     1561 gtaaagttgc tggaaataaa tttataaagt tgtttggcac cattttgggt ttcttgggga
     1621 ataacattca ggtatttcaa gaaggctgcc agaataatca tcaacaccgg tcctggaaaa
     1681 cctgttaaat gctgtaacaa gccaccagca ataaaaagtg aacaagctgt taaaacgcct
     1741 gcccccatta atttaacatc tagggctcca gaattgtctt ttagggcatc agacatgtct
     1801 tcgctgtgac caattttaac cagttgccct tgaccagaat aacttggatg cttctctcca
     1861 aaacgattga gcaaagccgt gcacaagata gcaaagaaat tcccaataat ggtggctgga
     1921 atcagttgag ccactaattg ctcacttccg actcctgtaa tggcactgta accaagagat
     1981 aagggtaaaa taccttcacc aattcctcca gcaaggactg gtgtcacaac ataaaagagg
     2041 gtatgttgcc agtctaatcc taaaatgacg ccaaccaaag tccctacacc cattgcacaa
     2101 accattccaa gtaacatggg aataatcatc cgaaaaagac cttggatcaa gatttttcga
     2161 ttcatgccta aaatactacc gcagaccagg caggcaatat aaaaataaag aaaattggct
     2221 tgcttcatca agacattcgt cgcttctaag acattggggt tgaggagatt aaaaaaaacc
     2281 aaaatcgaag ggactaagag tgataaaata gctgggccac caaaatgttt gaggcctgga
     2341 atggtcgctc caatggttcc tagtagccaa ccaagggtta aaataacagc aaagccaccc
     2401 agcatattaa caggcaactg ttggagccag cctgtgacta aaattaaggt agctaaaacc
     2461 aaataaacag gcaagggaac ggagcctatt cgcctatttt gccatgcttt tgaatgctca
     2521 gacaagtctt tttgaggcat tttcttgctt attgtttcca tgaaacaacc tcctttagtg
     2581 ataaggctag tataaaagag aacatgtcaa aagcatagtt tatttaagtt gtttaactac
     2641 tttaattttt tcaagttatt taattaattt aagctagcag gtcatttctt atcgtgttat
     2701 actcaataaa gaggagacct tatgaaaaaa ccactacgtt tatgggccag tttatcgttg
     2761 attttggtat ctatgattgt tgtcacaacc tctctatttt acgggattat gctacatgat
     2821 acgcaccaat ccatcaaaaa tcaggaaacc catttgctaa cgtcaactgg aaaaatgtta
     2881 gcaagtcatc aagcaattaa agaattactc ctaaacaacc aaccaaatgc taaaacaaca
     2941 gcctatacca actccattgc ctccatctat aaccttgatt atgtggtcgt gatgaacatg
     3001 aaagggattc gactcaccca tcctaacccg aaaaatatcg gaaaaccttt tcaaggaggt
     3061 gatgaggagg ctgttctagc aggaaaaaag gttatctcta ccgccaaggg cactttagga
     3121 aagtcacttc gctatctagt acctgtgttt gacggtgaca aacagattgg tgctattgcg
     3181 gttggcatta aactaactac cctaaatgat gtggctttaa cgtcaaaacg caattatacc
     3241 ctgtctttac tcctttgtct tcttattagt ttactggtca ccagttttat ttcttttcga
     3301 ctcaagcgtc aactgcacca gttagaacca agtgaaattt accagctctt tgaagagcgt
     3361 aatgccatgt tggatcaaat cgaagccgcc gtatttgtgg tagataaagc tggtatctta
     3421 cagctctgca atcaggctgg acaaaaatta attgctcgga aatgccagtt gggaaaacct
     3481 actggcaaca gtttcaatca cctctttcct gattttccaa aacttagttt gcaagagggg
     3541 catgaacaac tctttcggta tggtgaggaa gactacttgt tagccatttc acccatctgt
     3601 gtcaaaaacg atcaccgagg ccacattatt ttcatgcgag aagcagttaa ggctattgat
     3661 accttggatc agctagccta taccactgcc tatgcttctg ctctacaagc ccaaactcat
     3721 aaattcatga atcaattgca tgttatttat ggcttagtgg atattgctta ttatgaccaa
     3781 ttgaaaattt atttagacag tattttggaa cccgaaaatg aaattctcac tagcttatct
     3841 gtccttgtta aggagcctct tctagctagt tttctcattg gagagcaaga gaaataccaa
     3901 gaattaaatg tccatctcaa gattgatgtt ttaagcgaga ttcctcattc tgccaccaag
     3961 aatcaactta ataatggttt gatgatttac cgctttatcc atactaatct tctaaccaca
     4021 ctgagaccca aatcactcgt tttgagtatt caacatgatc agaatcattt aatcagccat
     4081 tacaccttga cagacaattg gattgatttg gagcgggtac agcctatttt tgacttacct
     4141 tattttcaac agctcttgac cgatacaaat tcccaattcc atcaacatca aaccgtaacc
     4201 gagttacagt tctcagtgac aacaccttat gtaggagggc aacatgaacg ttttaatcat
     4261 tgaagatgat cctatggtgg attttatcca tcgcaattac ctcgaaaaac taaatctttt
     4321 tgatcgcatt atatccagcg actctatgaa agctgttcaa agcatactta ctgattacgc
     4381 aattgacctc attttactgg atattcatat tacagatggc aatgggattc aatttttgga
     4441 gaaatggcgt gctcagcata ttccttgcga agtcattatt atttcagccg caaatgatgg
     4501 gaatattatc cgagatggtt ttcatttagg aattatcgac tacctcatca agccctttac
     4561 ctttgaacgc tttcaagaaa gcatccaaca gtttgttacg catcgagaac atttggccaa
     4621 tcagcaacta gagcaggccc aaattgacca actgaaatgt ttgactagca aaaaagacac
     4681 taaaaacaaa caattgttag aaaaaggctt atctgaatcc actttccagt ggattatgga
     4741 aaatatcaaa gcctttgatc aaccttttac tatccaagaa ttagcttctg cttgtcactt
     4801 atcccatgtc tctgtccgta aatacattgc ttatttagaa gaaaataagc aactgaacag
     4861 ccaacaaatt tttaccaagg ttggccgtcc ctatcgcgtg tactggtgac gtcatctgtc
     4921 aatacattaa aaaggctgga gaaataccag cctttttgag tgcttatttg cgatacattt
     4981 taaattggag gtataaatca ttataaatcc ccaaccatct tgaccctaaa ttatcataaa
     5041 cttccaattt tttgataatg tcatctgttg gataaaaagc aggatcattt tttatctcat
     5101 ctggaagtaa ggccttggct tttttatttg gtgtcgcata accaatatat gcagcatttt
     5161 gcgcagcatt ttcaggacga ttgataaagt tcaaaaaagc ataagcttct ttttcgtgtt
     5221 tcatggtttt tggtagtacc aaattatcaa accaaaggtt agacccttct gaaggcacga
     5281 tgtagtgaag gtgttcgtta ctatctaaca tctcactggc ttcaccagaa aaggtaattc
     5341 caatagcagc gtcaccttga atcatgtagc ctttcatctc atctgctaca atggctttaa
     5401 cattcggcgt caactgctgc agttttctct cggctgcctg caactgctct agatttttag
     5461 aattcacact ataaccaaaa gttgttaaac caacccctag catttcacgc gctccatcaa
     5521 tcagcataat actattttta tattctgggc gccacagatc ttcccagtgc ataggcgcct
     5581 tatcaactaa ttgatcatta taaacaatcc caacggttcc ccagaaataa ggcaaagaat
     5641 agtcgttttg tgggtcaaag cttttcccta aaaattcttt cccgatatta tccatgccaa
     5701 ctaattttga cttatcaagc ttattgagta ggttttcttt gatcatttta tcaatggtgt
     5761 aatcactagg aacagcaatg tcataagtgg ttccgccctg cttgatttta gtgtacatgg
     5821 cttcattgga atcgaaagtt tcatactgca cttcaatgcc cgtttctttg gtgaattttt
     5881 tgagcaaagc tggatcaatg taatctcccc agttataaat aactaattta tccgattgac
     5941 taccagaacc cgattttttc tgcaagatga aagaaagact tgttaaaata acaataacac
     6001 ccaaaactcc tgctagaaag gaataaagtt tacgcatgtt tttcctcctt gtcctgtgac
     6061 atataataat aaccgatcac taagaggatg gagaaaaaga aaacgatggt tgacaaagca
     6121 ttgatatcca aggaaattcc ctgacgagcc cgcgaataaa tctcaacaga taaagtagta
     6181 aaaccatttc cagtcaaaaa gaaagtcact gcaaaatcat ctaaggaata ggtaaaggcc
     6241 ataaaataac cagcaataat ccctggtgtt aagtatggca gcatgacttc tttgagcatt
     6301 tggaaataat tagctcccaa atcataagcg gcgttgacca tgtcctgatt catctctttc
     6361 aagcgcggca ataccatcaa aaccacaata ggaatcgaaa aagcaatatg acttaataaa
     6421 actgaagaca tgcccagctg aaacttcaat gatgtaaaaa gaattaaaaa ggaagcccca
     6481 atcatgacat ctggtgatac catcaagaca ttattggctg atagcatggc attttggtac
     6541 ttacctctaa catggtggat aaagatagca ccaaaggtcc caataatcgt tgctagtaga
     6601 gcgctactaa aagcaagaac aaaggtttgc agtaagattg tcatgagacg actatcctca
     6661 aacatggttt ggtaatgctc aagggtaaat cctgtaaaac cattcatatc cccacctttg
     6721 ttgaaagaat agaaaatcaa ataaaaaata ggaatgtaga gtaaaacaaa gacactcgct
     6781 aaataaagat tggcaaattt tttcatttac ttttctcctt tgtgagccac ataatagcaa
     6841 ccatggtcaa aatcaagacg acaccaattg ttgatcccat tccccagttt tgggtggtca
     6901 aaaaatgttg ttcaatggct gtaccaagtg taatcacccg gtttccacca atcaaacggg
     6961 ttaacataaa gagactcaaa cttgggataa agacagactg aacacctgcc ctaaccccat
     7021 ttaaagaaag gggaaaaata acttttgaga aggcctgaaa ttcactagct cctaggtcgc
     7081 gactggcatt gatgacatta tggtcaatat catccaaagc gttaaaaatc ggtaacatca
     7141 taaaagggag ctcaatgtaa gaggctacaa aaataaagga gaaatccgtg aacaggattt
     7201 gctgcgggcc aatccccata aaagttaaaa agctgttaat tcctccttgt tgaccaaaga
     7261 ttcccataaa ggcataggct tttagcaata agtttaccca agttggcaaa ataatgagca
     7321 taagccacaa ctgcttgtgc tttaggcgcg ttaaaaagag agccgtagga tatgaaatca
     7381 agagcgtgac gagtgtgata ataccagcgt ataaaataga attcacactc attctcaaat
     7441 aggtccaaga gctaaaaaag gtttcataat tggctaaggt cacgcgccct tctatgtcaa
     7501 aaaaggactt gtaaaacaag agagtgactg gtgccacaac aaagaagaga atccataaga
     7561 agtaaggaat cgaaaaaaga ctagaggttt tcttcattac gctcctcctc aatggcatta
     7621 atcaatccat cttcaggctc atccatttcc acatattctt caatacgggc atcaaattcc
     7681 tcttcagtct cattaagacg catgatatga agatcttcag gggtaaaatc taatccgata
     7741 acttctcctt cgatagcttt gcgggtagaa tgaatcatcc attcattacc caattcatca
     7801 taggcaataa tttcgtagtg aaccccgcgg aataattggg tatcaacctt aacttgtaat
     7861 tttccttctt ctggcaaagt aatttgaaga tcttcaggac gaataaccac ttcaacaggc
     7921 tcattagggc gcattccacc gtccacagat tcaaattctt tcccgttaaa ggagacaaga
     7981 tagtcttcaa tcatggtacc gttaataata ttagattctc caataaaatt agcaacaaaa
     8041 tgattaattg gctcatcgta aatatccact ggtgttcccg actgaacaat ttcaccttca
     8101 ttcatgacaa aaatccaatc actcatggcc aaggcttctt cttgatcgtg agtaacaaaa
     8161 acaaaggtaa tacctagacg ttgttgcaat tcacgtaatt catattgcat ctctgttctt
     8221 aatttcaaat caagggctga gagtggttcg tcaagtaaga ccacacgcgg ttgattgata
     8281 atcgcacgcg caatggcaac acgttgacgt tgaccaccag ataacttctg gatagaacga
     8341 ttttcatacc cttctaattg aaccattttc aaggtttctt tcacgcgctt agcaatctct
     8401 ttcttgtcca ctttcttcag cttcaaagca aaggcaacat tctcaaaaac agtcatatga
     8461 gggaagaggg cataattttg gaaaacggta tgaatgtcac gtttattaat cggcaaatca
     8521 ttaatacgtt ccccatctaa ataaatgtct ccgctgctgg catctagtag gcccgccata
     8581 atatttaaaa tagttgattt tccagagcca gaagctccaa gcagggtgta aaattttcct
     8641 tcttcaaggt caaagttaat gttctttaga acctgtgttc cactgtcctc aaatgttttt
     8701 gaaacattgt taaatgtgat aattggctta gtcaattgtc ataaatcctc cacggtactt
     8761 tctttaatta tcaataatgg ttacaggttt tccccaataa tccgaacttc tggttcaagc
     8821 ctgacaccag aatggttttc aactgtctca atgacataag caatcaaatc ttcataatct
     8881 ttagctgtgc catctgccac attaatcata aaaccagcgt gtttttcgga aacttccaca
     8941 ccaccaatcc gatgcccttt aagatttgct tccatgatta attgacctgc aaagtgtcct
     9001 ggcgggcgct taaacacaga tccacaagat ggaaattcta aaggttgttt tagttggcga
     9061 aggtgattta accgattcat ttcttggctg atcgtatcat aattacccgg tttaagagca
     9121 aacttagcag aaatgacaat gtcacctgtt tcttgaatgg cagagtgacg gtaaccaaaa
     9181 gccatatccc tagctgaaat ggttttgatc tctccacttg aggtaagcac cttagcggat
     9241 aagaaaatat gtgatatttc cccaccatag gcacctgcat tcataaaaac agcaccccca
     9301 atgcttccag gaatgccaca tgcaaattca aaccctgtta aactatggaa tttggcaatc
     9361 ttcgttgttt caatcagatt ggcaccagct tcggcttcta aggtataacc atttaagtgc
     9421 actgcattta gcttatcaaa catgatgaca aagccacgaa taccaccatc tcgcacaatg
     9481 agattgctgg catttcccaa aacaagccaa ggcatatttt ctttgttggc ataagcaacg
     9541 atgcgtgata gctcgtagtg atttctagga aaagccaaga aatctgctgg gccacctacc
     9601 ttagtgtaag tgtaatgttt taggggttca ttctctcgaa tatcaatccc atgaagttca
     9661 gttatcatag ttatcttctt tcttttatca cttcttatac acgtaaaaaa atcggcataa
     9721 agccgatact attatatcaa attttagaaa aaataacgat gaatttggca aaactttatg
     9781 gattggctta tagcaggcat tactccgaaa acagagtcta gaactctgtc tgttgagctg
     9841 aaaagagtgt gatatctgat tgatctaaag ctgcaagata atcccttaaa aacttatgat
     9901 taggaggcaa ttttaaatct ggctgcaatt ccaataaggg gattaaaaca aaagcacgct
     9961 cagtcatata agggtgtggc actttcaatt cctttgtgtc ataaacctct tctccaaata
    10021 gcaagatatc aatgtcaata gttcttgatc cccatttttc atgcctcaca cgaccaagag
    10081 attgctcaat agcttgtgtt tcttttaaga aatcagccgc ggttaattgt gtatctaatt
    10141 gacaggccat attgagaaaa tcagcctggc ctgtcttgcc ccaagcagtc gtttcataaa
    10201 tggatgattg agcaagcaac cgtgtttgag gtaagtcagc taaagcctca agagcttttt
    10261 gcaagtaagc tgctcggtcc cccatattgg tgcctaaact taaataaaca atggtcatct
    10321 ctgtctctcc aattcaatac caactgcctt atagtggcct gcaatcggtg ggttttcctt
    10381 tttaatggcc actttgatgg cttcaatagg tggaaattcg ttaaaaagct gttcacaaat
    10441 cgcacctgct aaacgttcaa tcaaaataaa ctttccccct tccactaact gacggacact
    10501 atcaaacacc atcccgtaat ggaccgtatc tgttaattgg tctgacaaag aagctgcttg
    10561 caaatcgact gacaattcta aatcaaccag aaaaatttgc ccaagggtct gttcttcttt
    10621 aaaggctcca tggtagccat aaaaacggca accttctaat acgattttat ccataagtcc
    10681 tcacatcaac tagctcaaaa cagccacaat gtcttgatta gccttgacat cgtgtacgcg
    10741 tacaatctga cagccttttc caagggcata agcagacaag gctgctgtcg ctccgtctcg
    10801 ctctttagct ttggtgttgc cgcctaacaa ggcatctacg acacgctttc tcgaaatacc
    10861 aaacaagaca ggataaccca actgacagac gcggtccaat ccttttaata actccatatt
    10921 ctgttgaaca gatttggcaa atccaaatcc tggatcaatc caaatattgt tttttggcac
    10981 gccagcatca agagctgctt gtgctctatt gcctagaaaa tcacaaacgt cttgtgttac
    11041 ctcttgataa acttcttcgt cttggttatg catcaagatg ataggcgcat cgtactcggc
    11101 tgctaaggca aacatttgac cgtcgtacaa acctgcccaa acatcattga gaatatcggc
    11161 acccgcctct aaagctgctc tagctgtttc ggttttatag gtatcaatgc tgattagaat
    11221 atcatagttt tctttgatgg ccttgatcac aggaaccacc cggtcaattt catcggtagc
    11281 gcttacaaat tggcaacctg gacgtgttga ttctccacca acgtcgatga ttttagcccc
    11341 atcagcaatc atttgctcaa cgtgatctaa tgctttttgc acagtagtgt aagacccccc
    11401 atctgaaaaa gaatctggag tcacatttaa aatccccatg atagccgcat tgccctcaat
    11461 cacaaacttt ccaattttca taagttctcc taatcttttg tcattagaga aataacttct
    11521 tgcctctcag cacgactttc cttatataag cctctggcag tggtggtaat ggttttacta
    11581 cctggctttt tgatgcctcg catggtcatg cacatgtgct ctgcttctac cataactaaa
    11641 gtccctttag gattgagggc ttcaactaaa gcatccgcta tttgagaggt caaacgctct
    11701 tggagttgag gtcgtttact agctacttca actgctcgcg ctaatttact caaacctgtt
    11761 actcgaccat cacttggtaa ataagcgata tgagccttgc cataaaaagg aactaagtgg
    11821 tgttcacata tagaataaaa actaatgtct ttgacaatca ccacatcctc atgctgttct
    11881 ttaaagacag ccgtaaattc ctctttagga tcttttccca gtcccaaaaa catctccgca
    11941 tacattttag caacccgttt aggcgtgtca agaagacctt cccgatttgg attttcacca
    12001 atagcctcta aaaactgata aatggctgct tctgcttttt ccttatcaat agacattagt
    12061 ctttcccttt tcattagttt tttccttttc attttctctt ttttaatggg ttaaaagaac
    12121 gcttttttca atatgttttt tccaataccg tctaatttct gaaataaaat atagtgaccc
    12181 tgtaatcacg aaaaagtcat ctgctttagc atgcttacgc agggccaaga aatctttgaa
    12241 atcagcaacc ctaccaaaac gttctgggta tttttccaat ggataggcgt tagggtaatg
    12301 aaagctagta acctgcaagt cacctatttg ctcaagagca accaacatat ctgcaatagg
    12361 cttggtgtcg atcgcaccaa acaaaatatg aagcttttta tcattgtagt tattttttaa
    12421 gacggctacc aaagcagcaa cgctttcatt gttatgagcc ccatcaatca ttagatttgg
    12481 tgctaataat tcggtacggc ctagccacaa actatttgct agaccttccc ttatatgatc
    12541 tgaagttagc ctaggatagc ggtctttcaa ggttaagcaa gtcataatag ctaaagctgc
    12601 attagacact tggtggtgtc ctggcatagc gatgtgaata tctgaaataa ctcctaaagg
    12661 actagtaaaa cgatagccag atgcgttctc agccatttga aactgctctt gccattccca
    12721 gattgaagca ccaacctgct cagcttttgt taaaaaaacc tctcgagctg acgggttttc
    12781 aacggcaaaa acaagggtct cgcccccttc taaaacacca gctttttggg cggcaatatc
    12841 agcgtaagtc tctcccaaaa tagcttggtg atctaaacca attgagggac agaccacaac
    12901 cattgcttga aaaacattgg tcgaatcata aagaccgcca aggcctgctt caataatagc
    12961 aatatctaca ggatgcatat caccaaaata gaggaacatg atcaaagtaa tcacttcaaa
    13021 ttcagttacc tctccaaaat cagtctctcg aactagacgc tctgtcaaag gacgaatacg
    13081 attagcagca ataaccaaat ctttttctga aatcattcga ccatttatgc taatacgttc
    13141 tttgaaatcc ataatataag gagaggtaaa ggtccccact tcataaccag ctgtcgtaaa
    13201 gatgtgttgt aaatgattga ccgtggaacc ttttccgtta gttccaacga tatgcactcc
    13261 cttgacattt ttttggggat ttcctaactg tccaagaacc caaagcatac gttttaagcc
    13321 tggcttgatg ccgaaaacta agtgatcatg aatccattct agagtctctt cataagtcat
    13381 ttcatacctc acatctatct ttagtatacc atatataaaa aaaagtgaca gcagataacc
    13441 tagagtagta cccttttttt tcttatcctc accaaacact caaataagtt ctaaagtaca
    13501 ctacactttt atcttcattg ctttttttac gtgataagaa taccagtccc acatagtctt
    13561 tgctacctcc taaagtgtta tctgtgacaa caattttttt actccttaat acacgttctt
    13621 ccaaaaaaca agaaaaaagg ctgacttcaa gccttctttt ctctaaaaaa tgttacaata
    13681 gaggtactta tatcaaaaaa aggagcaagt aatgaacaat agacataaac ggcgccaaaa
    13741 aagaaaagcc ttcactctca ttaaccttat actattattt gcctgcattc ttggtttgac
    13801 cctcattttc tatttatatc aagggtctaa caaattcggc ttgtcagatg tctctcaatt
    13861 tgtacctaaa ataagtcttt ttcatcacaa aaagacggca aaaaaagaaa cgacaaaact
    13921 taagaagact catttcgatt cctcaaaatc tcaaaaaaag gcccattcta aattaacttg
    13981 gaccaagcaa gaaacacctg tcaagatccc aattttaatg tatcatgcta ttcatgtaat
    14041 gtcccctgaa gagacggcca atgctaattt gattgtcaac ccagatctct ttgatcaaca
    14101 acttcaaaaa atgaaagacg agggctacta ttttttgagt cctgaagagg tctaccgcgc
    14161 tctttcaaat aatgaattgc ctgctaaaaa agttgtatgg ctaacatttg atgacagtat
    14221 gattgatttc tacaacgttg cttacccaat cttaaaaaaa tatgatgcta aagccaccaa
    14281 taatgttatt acaggactaa ccgaaatggg aagcgctgcc aatctgactc ttaagcagat
    14341 gaaagaaatg aaacaattcg gtatgtcttt ccaagatcat acagtgaatc atcctgatct
    14401 agagcaggct agtcctgatg ttcaaacaac tgaaatgaaa gattcaaagg actatcttga
    14461 taagcaatta aaccaaaaca ctattgccat tgcttatcca tctggtagat ataatgacac
    14521 taccttacaa attgcagcta gacttaacta taaattaggt gttaccacta acgaaggaat
    14581 agctagcgcg gctaatgggc ttctttctct taacaggatt cgtatcctcc caaacatgag
    14641 tccggaaaat cttttacaaa caatggaacc ataacatcaa tttttttatc attccaaatc
    14701 atactcactg taaactaaaa agacagttat gaaaaaattc tttcataact gtcttttttt
    14761 gattgtttta aaattatttt attcttatct ttaacgtctt ttttcaagaa aatctcgcca
    14821 ccacagctgt aagtcagagg cttttttaag agattggcta gctttaagag gaatacgctt
    14881 aggttccttt aaatacctca ctgactgatc tgttattctt ggagacgtca aataagcaac
    14941 cacttcacct ttagtaatcg gtgctagtaa agaagtctct gatatcataa attgtttagg
    15001 tttgggaact tgttcaccct gttgcaaaat gatggttaca tcttcggcaa cagataccag
    15061 tagttctttt acttggccat cttgaatagg gagcgaccgc tccgagacaa gacttccttt
    15121 tttaagaatc ttacgacgac aataagttct agccacatag tccaacaaac gatttgcttg
    15181 ctcaaaccgc gtgttttggt ttttatcaga tccatcggca tttagaataa cggtaatcac
    15241 tcgcatacca ttttcaatag aagttaccac aagacaatga cctgccaagt ctgttgtccc
    15301 agtctttaaa ccatccactc ctgctctacc cttagccata cctggtaaga gttgattaaa
    15361 acttttaata gaattgccag caaaattaac ttctgtttgt ttggtgatct ctaaaatctc
    15421 aggaaaatct tctaacaaat gcatcgtcac aactgcaaca tcttgagcac tcaaggtatt
    15481 ttcttcttcc aacgcagaac caggataacg attatccttt aacacctcat ttggcagacc
    15541 tgaggcgttg aggattttac cagaagtaat cccccaatga gatagctgtt ctctcatttg
    15601 atttacaaaa tgaagctctg aacccgccac tttttcggct aacgcaatag cagcgctatt
    15661 tgagcttgct accaaagtag ctgtcaacag gtcttgaact gaataagttt ttttgtcaaa
    15721 aggtacatta cttaaagacc tatcttttgt taactcaaag gcataatctg agagattaac
    15781 ttgatctgat aattgcagct ggcctgattt aaccttatct aaaaccagat aagccgtcac
    15841 caatttggtc aaagaggcaa tagcatctgg cgtcttagcg tctttctcat acagaactct
    15901 cccagagtct gcctctacga caatcgcatg ttctgctccc acttgaaaat cagctgcgag
    15961 aactgtttta ccaaaccctg aacaagttaa taccataaaa aaaaggataa cccacctttt
    16021 tatcattata tctttcatag ttactattat aacaaaaatt ctcagaaatt tggaaggaag
    16081 tatagtggca cacaccttta gtagcttaaa caagctcata aagcaagtac cctgtgacaa
    16141 taacacatcc tgttatatgg gcgatataat aagcttcgtt atatcaatta gttgtttggc
    16201 aggaattttg gtataaaatg gaatctaatc ctgaacttat tacattttgg gttatttctt
    16261 cagatactgt tttatctaat gcattaaacc agtgactata tgtattcaaa gttgtagact
    16321 tatcagcatg tcccatacac tttgcaacgt acagaatgtc ttttttcaaa acattaataa
    16381 gataggaagc atgactatgt cgcaagactt ttcccgttat cactggtact cctacttctt
    16441 gagctttgtg cttaataact cgacaaatcg ttgatttaca aaatggttca ccaaaccgtg
    16501 aaataatgaa atctgtgtca gtattgacta cttgatttct tctccaattt tccaatacta
    16561 ctattgtcac atcatctaac tcaataagac gttctcctgc tggcgttttt gtttgatctt
    16621 tacgatacca atttccattt tcatcctttt ccaaggttgt atgaactttc aaaaattttt
    16681 gttcgaaatc gatatcttcc catcataatg ataaaccttc acttacacga acacctgtca
    16741 tataatatag ccaaatagtt gtaaatcttt gtagctcttc ataatcttgc aaatcaaacg
    16801 attttataaa attctgaaac tcaatataag tccaaaatgg agtatcggga tgtttccctt
    16861 taggattatc caaagcctta cacggcactt ttgaaatata gtctaacctt tcagcatacc
    16921 ccatgcaagc tttgaatcgc gaccataaat tctttgcata attttcaata atatgtaaac
    16981 taaaagattc acaatctcaa gctgttattc cctttaaaag tttagaacca aaatactgaa
    17041 taaataactt atgatgtggt aaggctattt cataagttac agcctgaacc ttttgcttat
    17101 atgctctt
//
LOCUS       DAUELZ010000033        16788 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.033, whole genome shotgun sequence.
ACCESSION   DAUELZ010000033 DAUELZ010000000
VERSION     DAUELZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16788
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.033"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..363
                     /locus_tag="VHI09_000827"
     CDS             <1..363
                     /locus_tag="VHI09_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6120715.1"
                     /translation="KTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKGKYTP
                     KSGLLSDIAAIFDVSLTALMEEDLTLESKGVSYQLPQRELAMISEQLSENNYTKWVDF
                     AQILLEQQAKEDRELDNH"
     gene            556..855
                     /locus_tag="VHI09_000828"
     CDS             556..855
                     /locus_tag="VHI09_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEQ6120716.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDETTKKTLGAFAGKLPF"
     gene            complement(912..1205)
                     /locus_tag="VHI09_000829"
     CDS             complement(912..1205)
                     /locus_tag="VHI09_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ6120717.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTAGGRRLYT
                     DSDLEQLRMICFLRDLGFSIAGHTPRTRKLTCSHCHQKSYCLELAKEDTVSRG"
     gene            complement(1358..1945)
                     /locus_tag="VHI09_000830"
     CDS             complement(1358..1945)
                     /locus_tag="VHI09_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEQ6120718.1"
                     /translation="MQHLDTYIAFDLEFNTVNDVSHVIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            complement(1994..2524)
                     /locus_tag="VHI09_000831"
     CDS             complement(1994..2524)
                     /locus_tag="VHI09_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEQ6120719.1"
                     /translation="MGIEKTVSELADILGVSRQTVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFFTRLFAK"
     gene            2729..3898
                     /locus_tag="VHI09_000832"
     CDS             2729..3898
                     /locus_tag="VHI09_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ6120720.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDDLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGKVLTNTEVVSVKKQDDLFYLKSADQTFSCHKLIV
                     TTGGKAYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSENYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
     gene            complement(3993..4664)
                     /gene="deoC"
                     /locus_tag="VHI09_000833"
     CDS             complement(3993..4664)
                     /gene="deoC"
                     /locus_tag="VHI09_000833"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEQ6120721.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECEDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAETFIALGASRLGTSRIIKIVKNEAT
                     KTDSY"
     gene            complement(4694..5896)
                     /locus_tag="VHI09_000834"
     CDS             complement(4694..5896)
                     /locus_tag="VHI09_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEQ6120722.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            complement(5917..6696)
                     /gene="udp"
                     /locus_tag="VHI09_000835"
     CDS             complement(5917..6696)
                     /gene="udp"
                     /locus_tag="VHI09_000835"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEQ6120723.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            6937..7695
                     /locus_tag="VHI09_000836"
     CDS             6937..7695
                     /locus_tag="VHI09_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ6120724.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     EITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSCQSDTH"
     gene            7915..8184
                     /gene="rpsN"
                     /locus_tag="VHI09_000837"
     CDS             7915..8184
                     /gene="rpsN"
                     /locus_tag="VHI09_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEQ6120725.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(8355..9383)
                     /gene="tsaD"
                     /locus_tag="VHI09_000838"
     CDS             complement(8355..9383)
                     /gene="tsaD"
                     /locus_tag="VHI09_000838"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEQ6120726.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGNELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(9373..9828)
                     /gene="rimI"
                     /locus_tag="VHI09_000839"
     CDS             complement(9373..9828)
                     /gene="rimI"
                     /locus_tag="VHI09_000839"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEQ6120727.1"
                     /translation="MIVTTLSESNMKTVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIR
                     RDQTDYFLLYDHDKLLGFLAVQDLAGEVEMTQIAILPSHQGLGLASQLMTHLDSIESD
                     IFLEVRESNHRAQGLYQKFGFKLIGKRPDYYRNPIETALLMKREGKHDR"
     gene            complement(9800..10498)
                     /gene="tsaB"
                     /locus_tag="VHI09_000840"
     CDS             complement(9800..10498)
                     /gene="tsaB"
                     /locus_tag="VHI09_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEQ6120728.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHASLEVIIEQLVKEGQLIFVGE
                     TTPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            10782..11012
                     /locus_tag="VHI09_000841"
     CDS             10782..11012
                     /locus_tag="VHI09_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEQ6120729.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            11014..12696
                     /locus_tag="VHI09_000842"
     CDS             11014..12696
                     /locus_tag="VHI09_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ6120730.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(12924..14270)
                     /gene="glnA"
                     /locus_tag="VHI09_000843"
     CDS             complement(12924..14270)
                     /gene="glnA"
                     /locus_tag="VHI09_000843"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEQ6120731.1"
                     /translation="MAITVADIRHEVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDETDKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPVEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFVSQWEIDHYIHNY"
     gene            complement(14308..14679)
                     /locus_tag="VHI09_000844"
     CDS             complement(14308..14679)
                     /locus_tag="VHI09_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ6120732.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            complement(14746..15297)
                     /locus_tag="VHI09_000845"
     CDS             complement(14746..15297)
                     /locus_tag="VHI09_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ6120733.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKSGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWFNKKHSDVR"
     gene            complement(15560..16756)
                     /locus_tag="VHI09_000846"
     CDS             complement(15560..16756)
                     /locus_tag="VHI09_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEQ6120734.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
BASE COUNT         5254 a         3493 c         2889 g         5152 t
ORIGIN      
        1 aaaacttttt ttgcaagtaa tttaaagtac cttagattaa aaaagaacat ggaacaatta
       61 gaacttgcta atctcctagg acgaaaaagc tcttcttcaa tcagtgagtg ggaaaaagga
      121 aaatatactc caaagtctgg tctattaagt gatatcgctg ctatctttga tgttagttta
      181 accgccctaa tggaagaaga tttaacctta gaaagtaagg gagtctctta tcagttgcca
      241 caacgagagt tagcgatgat ttcagaacaa ttatccgaaa ataactacac taagtgggtt
      301 gacttcgcac aaattctttt agaacaacaa gctaaagaag atcgtgaatt agataaccac
      361 taagtcggac tagtctatga caaaaatagt ccatttctag acttattctg tcattagcca
      421 gacccttcct actaatctat cttatccata gcgactttct tactgataca ggctagtcta
      481 ctaactgaca ttgtttttat tgcctctttt tatgtgttat aatagttagt attaaattaa
      541 ggagagtata gtcttatgat gaatatgcaa aacatgatga agcaggcaca aaagcttcag
      601 aaacaaatgg aacaaaaaca agcagatttg gctgctatgc aatttactgg aaaatcagct
      661 caagaccttg tcactgccac tttcacgggt gataaaaaat tggttgggat tgacttcaaa
      721 gaagctgttg tagatcctga agatgtagaa acgcttcaag acatgaccac tcaagccatt
      781 aatgatgccc ttacgcaaat tgatgaaaca actaagaaaa cattaggcgc ttttgctggt
      841 aaattaccat tctaaaaaaa caaaaactag cttgagaagg atctcaggct agttttttta
      901 tgagttagtg cttatcctct tgagacagtg tcctcttttg ccagttccaa gcaatacgat
      961 ttttgatggc aatgggagca ggttaacttt ctggttcgtg gtgtatggcc tgcaatggaa
     1021 aatcccaaat cgcgcagaaa gcaaatcatg cgtaattgtt ctaaatcaga atccgtatag
     1081 agtcgccgtc cacctgctgt caatgctgtc ggaatcaaaa tcccacgttg gtcatagtat
     1141 tggacagttc gaatagaaac gccagctaag ttggccaatt ctccagtcga atagaattga
     1201 gacatttcca caccttcttt ctaccattta gtcatcaaaa gcgactcgtg tgcacccgac
     1261 tccctcttga tatgccccta gcttatcaca tgacctagcg ttacaagcaa gggctaaccc
     1321 tagaggattt ttttgttttt taaaaatagc agagccttta gtcgaaaaag tctcccaaag
     1381 ccgcgaaagg gttatctgtg gttacctctt cttgctggct gagatatgct ttgtttgtat
     1441 ctgtttctaa aaaagactta tagattgctg ctgtcattct ggcatcttct aagctattat
     1501 gacctcgacc cttaatgcct aaaaaggttg cgactgtttg cagacgaagg ttagcaatcc
     1561 catttaagtc tgcgctacgt ctatcgtaag cttcatcaaa gaggtcaatc tggtattggt
     1621 cccttaaatc taagccattt tcagctagaa taggcaggtc agatttttga gcattataac
     1681 caatcaatgg taactctccc acaaaatttt taaacgcagc cataacctcc tctacttttg
     1741 gtgccgcagc aatcttgtca gatgtgattc ctgttaggcc attgataaag ctctgtaagg
     1801 gaacatctgt gtaaacatag gtgtcaaagc tgtcaacttc tttatggtgg tcatatttga
     1861 ctgctgatac ttgaatgaca tgactaacgt cattgacagt gttaaattct aaatcaaagg
     1921 caatataagt atctaagtgt tgcatgtttt cctccaaaaa agaccctgat agggccttcg
     1981 tcattttttg tgattatttc gcaaacaagc gggtgaaaaa gcctttttta gggccaactt
     2041 cagcctgaac ttcttcgact tgtaagcgag cttggttggc ttctgcttta gcttcttcca
     2101 attctagttt tagggtttct ttatcagcca tggctttagc agttaattgt tgctgctgat
     2161 ccaattgttt atctttttca gcgatctgca cgtctttaac acgcatttgc tcatcttttg
     2221 aggcaagttg agcatctttg gccttgagtt gctcataaag gcgcgtgatt tcagtgttct
     2281 tctcatccac aagaatctct aaaagctcac gttgtttcgt ttcttcactg ataggctcat
     2341 cgtcaaaaat tgttttttta tagatctctt ccagcttaac aagaccactg cgcttaacca
     2401 cagtaacgcc tttttcattt ttgtcaagat cttcttcggg taaagactta acacgattat
     2461 tgacggtctg acgactcact cccaaaatat cagctagttc actaactgtc ttttcaattc
     2521 ccatattttc ctctttatca ctatcactga atattcttat taaaaatagt accataatag
     2581 gagtattctg tcaatttgac ctcatttcag tcgtgctaaa ggcatcaatt atttcttgaa
     2641 aacattgtta gatgaataga aaacggtatg ccaggttagt tcatttacct gactattaaa
     2701 tcccccgctt ttatgctaaa ataaggctat gactcaatac gatacgatta ttattggagg
     2761 tggccctgct ggtatgatgg ccgccatctc cagcagctac tatggctata aaactctctt
     2821 aattgaaaaa aaccgccgct taggtaaaaa attagcggga accggtggtg gacgctgtaa
     2881 tgtcactaat agtggtaacc ttgatgacct tatggcaggc attcctggaa atggccgctt
     2941 tttatatagt gttttttccc agtttgacaa ccatgacatc attgcctttt tcgaggaaaa
     3001 cggcgtcaaa ctcaaagaag aagaccatgg acggatgttc cccacaacag ataaatctag
     3061 gacaatcatt gatgctctgg agaaaaaaat caaggctctt ggaggcaagg tactaaccaa
     3121 cactgaggtg gtttccgtta aaaaacaaga tgacctcttc tacctcaaat ctgcagacca
     3181 gacttttagc tgtcataaat tgattgtgac cacaggaggc aaggcctatc cttcaacggg
     3241 ttcaacaggt tttggacatg atattgcacg gcattttaaa ctgaccgtga ctgatttaga
     3301 agctgctgag agccctctgt taactgattt tcctcacaaa gtcttgcaag gtatttccct
     3361 agacgacgtt actcttagtt atgacaaaca cgtcatcaca cacgacttac tcttcaccca
     3421 ctttggcctt tcaggacctg cagctcttcg tctgtcctct tttgtgaaag gtggggaaat
     3481 tgcagagctg gactttttgc cacacttatc aacagatgac ttaaccgctt atctgagtga
     3541 tcaacgtgat aaaaacatca aaaatgctct taaaggccta ctgcctgaac gagtagctga
     3601 ttttttatca gaaaattacc ccgaaaaggt caaacaactc tctccaaaac aagaaaagga
     3661 attgcttgat aaactcaaac acctccagat acccattaca ggaaaaatgt ctttggctaa
     3721 atcatttgtg accaaaggag gcgtagattt aaaagaaatc aaccctaaaa ccttggaaag
     3781 caagaaagtt cctggcctgt atttcgctgg agaggtctta gatattaatg ctcacaccgg
     3841 agggtttaat attacctcag ctctttgttc aggatggatc gcagggaagt cttcatagct
     3901 aaaaaagcct attatcacta agatagtagg ctttttaact cctattacat gagttttttg
     3961 ttttagtctc aggctgaatc ggtatgatta agttaatagc tatcagtttt tgtagcttcg
     4021 ttcttaacaa tcttgatgat acggctagta cccaagcgtg aagctcctaa agcaataaat
     4081 gtttcagcat cttccaatga tgagattcca cctgctgcct taattttaac accttcgccg
     4141 acatattttg ccatcacttc aacatcttca aatgtagcac ctgctgtcga aaaaccagta
     4201 gaggttttaa taaagtctgc acctgaacgt gtgacaactc cacaaagttc gataagttct
     4261 tctttagtta attgacatgt ctcaacgata acttttaaga tatggtcttg acatgcggct
     4321 ttgatttgac gaatttcttc ttcaacagta tcaaaatccc catttttaac gtctgtcaaa
     4381 ttaatgacca tgtcaatttc atcagcacca tttttaatag catcttcaca ttcaaaaacc
     4441 ttcgctgcag ttgtactata gccatttggg aacccaataa cagtacaaat agctaattta
     4501 cctgaaacgt attctgctgc ttttttgacg taagaggctg gaatacatgc tgaagctgtt
     4561 tcataagcca tggcatcatc taaaattgtt tggatttctg gccacgttgc tgttgttgct
     4621 agcaaagtat ggtctaccgt ttttaaaata tcttttactt ccacgttact atccttcttt
     4681 cttttgtcaa cgtttagaat agagttacaa taccgacgat aaaggcgcta agcatactta
     4741 cagcaatacc accaatcatt gcacggaaaa caagtcgagc tagggtgcta cgtttctccg
     4801 ggcaaagaac agcaatacct gaaacacaaa tacctaaact tgataaatta gcaaaaccac
     4861 agagtgaaat agttgctacc aatgctgtac gataatctaa agatttgatt aggtgaccca
     4921 attgttggaa cgaaacaaac tcatttaaaa tcaacttact tccaagaagg tttccttcta
     4981 gaagaatgtt tttgtggtca aatcccataa gaaaaccaaa tggagcaaaa acatatgaga
     5041 agatttgttc taagcgaatt cccaatccac ttaacatcat attaatcaaa gaaactaaac
     5101 caacaaaggc aatcaaacta gccccaattg agaaagccat ttgtgcacct gtgcttgcac
     5161 cctcagcgat tgcatcaatc acattggcgt tattaccttt attatccatc ttaatgtcat
     5221 caattttttg aacaggttct gtttgaggca ataagatttt agcaatgaga atactgccaa
     5281 taggaaccat tgttgaagca atcaagagat attccattgg aatgcctaat gcaatatagc
     5341 caccaagaat agaaactgac atacttccca tacctgatac caacacaacc attatctcac
     5401 tatcagtcat acgacctagg tatttgctaa ccaaaattgg actgtctgtt tgaccaagaa
     5461 acatattagc tacggcaaca aaactctcaa cctctgagga tttcataatt ttaccaacgc
     5521 ccttacctat ccattttact acaaatccaa ggattcctac ataataaagt agactaacta
     5581 gggcagataa gaaaacaata ttaccaagcg tttgaatagc gaaaataaaa ccagtttttg
     5641 cgccactatc tgctaatgac ccaaacacaa aatttaaacc agcttgacca cagttgatta
     5701 cgctagtaac tccagttgaa acaacactaa caatttgttg gcctagtggg atacgtacta
     5761 agattagcgc aataatgaat tgaacgataa gagcttttcc aattaaactt agagaaacac
     5821 tcttacgatt gaaagaaatt gcatacacaa ttcctaatac caataaaata ccaataatac
     5881 tataaataaa ttgcatgatg acttccaaaa cctcgcttat tgtgatttat cattttcaat
     5941 aagtgtgcgg agtgcttcaa cagcaacttg aatagctgct tctgtatcat gagccattgg
     6001 gttatccata cccaaagcgt tgcgttcttg attaccaaca acaaggaaat ctgatccaca
     6061 acgaactcct agatgacttg ctgctacaaa aagagctgca gattccattt cagacgcttt
     6121 tgttcccaga cgtttccatg cttcccattt atttaataat tcataactaa ctggcatacg
     6181 ttctggctca tgttgcccgt aaaatgcatc tttacattga actactcctg cgtgacttgt
     6241 ataaccaagc tttttagctg cattaactag agcatttgtc acttccaagt cagctacagc
     6301 aggaaattca atcggagcat attctttact ggtcccttcc atacggatag ctccagttgc
     6361 aataacgatg tctccgcctt taacatcaag gtcaatacca ccacatgttc caacacggat
     6421 aaaggtatca gcgccacaga gtttcaattc ttccatcgca atagacgctg atggaccacc
     6481 aataccagtt gaagtaacac taactttttc accgtttaat gtacctgtat acgtaacata
     6541 ttcacggctg tctgctacaa gaactgcatt atcaaaatgt tctgcaattt ttgcacaacg
     6601 ttttggatca cctggcatga taacataacg acctacatca cctggacgaa tctgtaggtg
     6661 atattgcaat ccgacttcac ctgaataatt ttgcatgtta aaaacctcct gtattttact
     6721 tgtctaattt ttgatacact tgtatagtat cacacacact ctattttgta aacgcttttt
     6781 taaggtgttt ttcatctctt ttttgacaaa aagtacctgg gtgtgagata ctaacactat
     6841 agatgggtac aattctaatc aaggaatact gttagtgcta aaatagtttt tagacaagtt
     6901 aaactccact aaagattatt aaatgaggta agtgatatgt ctactaacga cttaaccaaa
     6961 aaactcaaaa aacttaaaca tgtgcaagta tataatacta tttttcaact tattcaagat
     7021 ggtacatata gtcctggcat gcaactgcca tcagaacctg aacttgctag acagctcaat
     7081 gtcagtcgga tgacactgcg taaatcccta gccctcctcc aagaggatca tctcataaaa
     7141 aatatcagag gaaagggaaa ttttatactc aagactcctg aaactaaata tcaccaaggt
     7201 ttcgaatatc ttcaacaccc catatatgca agcctatcat ctgaaatcac aaaggttgaa
     7261 ttggaatatc ggattgaagt gcccactgtt gccattacag catccctaaa gcaagaaact
     7321 cctgttgtga ttattgttga tcgctggtat catagccaaa ataaagctat tgcttatagt
     7381 ttatctttta tccctattga ggttatttcc aaatatgcta taaatctcaa tcaagaagag
     7441 ccccttctta ctttcttaga agagaaaatc tatgaatctg gtaaagcttc tcattcctgc
     7501 aaccaaatcg gctataccaa gactggcaat tacacagcaa ctaagtatac tctatcagaa
     7561 aatagtgctt ttattttaat ccaagaaact ctctacaatg gtaaagacat cttggtctca
     7621 accaaacact acgttcctgc tgatttattt gacttaaaag ttcaatctca aagttgccaa
     7681 agcgacactc attaaacacc tttaaacagt tattaatacc tgactcccac acttagatgt
     7741 ggagtttttt gatattatgg ctaaaaaaga taactagtta atttttttat ttgcttttgc
     7801 ttttatttat tgtataatga ttatatatcc acttaaccag ttaaatacca gttaagtgga
     7861 ttcagagcgc tgataacgac tctgaggtta ttaaatatta agaaggagaa agcaatggca
     7921 aaaaagtcaa aaattgctaa ataccaaaag cagctccaac tgattgaaca gtacgctgac
     7981 cttcgtcgtg acttaaaagc gaagggcgat tatgaatcac tgcgcaaatt gcctcgtgat
     8041 tcaaatccaa accgcttgaa gaaccgcgac aaaattgacg gccgtcctca tgcttacatg
     8101 cgtaagtttg gtgttagccg tatcaatttc cgtgacttag ctcacaaagg acagcttcct
     8161 ggcgtcacta aagctagctg gtaagcttac ttcaccttag gcagacaatt attttgcagg
     8221 taattatctg aattattagt aaccatagaa gccaattact tatcatcatg acaacccggt
     8281 atgccaagat accgggttgt tttttcaaag ggagattctt atccattata aatcttacta
     8341 actagcaagt gtgactagtt tattacaaaa ctgtctggaa actggtcaaa agctaaacta
     8401 ggcttcgcgt ttaggctcat attagcaaaa tgctgtttgt catactcaat agctgctgct
     8461 aaagcaatca tacccgcgtt atctccacag agtcttagtt taggaatcac aacttctata
     8521 tgtgtaattt cttgtgctaa tctatctcgt aatccttgat ttgccgctac accaccagcc
     8581 actacaagca tctttgcagg gtatctgctc agagcttttt ttgttttggc caataaaata
     8641 tctagaacag ctgcttgaaa agatgcacac aaatcttcta gaatcagttc attgcctttt
     8701 tgcttagcat tatgatgaag attgataaaa gcggatttga gtcctgaaaa agaaaattct
     8761 aagtggtctt ctgttatcat agcacgaggg aaatgataag tatcttgacc tttatgagcc
     8821 aactgatcaa tttcacgtcc tgccggatag gttaagccca tcacacgccc aactttatca
     8881 taagcctcac caacagcgtc gtcacgcgtc tcgccaataa tatgataatc ccctggctct
     8941 gggacataaa ccaattctgt gtgtcctcct gaaaccaata gtgctattaa aggataaact
     9001 aaaggctttt gttcacgcgc tgccattaag tgacctgcca tatgattaac cggaatcaaa
     9061 ggtaaatgat ttgcccaagc aaaagccttc gcagctgcta atccaactaa caaggctcca
     9121 acaagtccag ggccataagt taccgcaact gctgataaat ctgaagcact gatgccagct
     9181 tcttgcaaag catcctcaaa gcaagtcgta atcacttcga catggtgtct actagctact
     9241 tctggcacca caccaccaaa gcgtttgtgg ctctcaacct gacttgcaat gacattactt
     9301 aagagggtac tttcgttttt taaaatcgct acactggtct catcacaaga actctcaact
     9361 gctaaaatat acctatctgt catgttttcc ttcccgtttc atcaataaag ctgtttctat
     9421 aggatttcta tagtagtctg gtcttttgcc aataagttta aaaccaaact tctgatataa
     9481 accctgtgct ctatgattag attctctaac ctctaaaaag atgtcacttt caatgctatc
     9541 taaatgcgtc atcaattgag acgctagccc aagtccttga tgactaggta gtatagcaat
     9601 ttgagtcatt tcaacttcac cagctaaatc ttgaacagct aaaaaaccta gcaatttatc
     9661 atgatcatat agtaggaaat aatctgtttg atcccgccgt atatcgatta atacttgttc
     9721 taaagtccaa ggagacgtgc cataaaccat ttccaaaagc tgataaatat tttttgcttg
     9781 ttcttcaact gttttcatat tagattcgct taacgtagtg actatcatct tttatctcat
     9841 tatctttgag ccagttttct tcagcttcca ctctcttgag atattgaggg acaaaggcgt
     9901 ctacattttc tggtgccaaa ctttgcccca aaagaccaca ttcgtaagca gaaggaaggg
     9961 ttggaagtag tatcgcctga ggtagtttct tttgaatttg ctcagcaaaa ggagtagtct
    10021 ccccaacaaa aatcagctgt ccttctttta ctaattgttc tataataact tctagtgaag
    10081 catgggcttg tggcatcact gattttcctt gccgataata acctacatac gcattttgcc
    10141 ttctagcatc aatcaatggc accaccaaag tatttggata ctgtttacaa gtagacgcag
    10201 ccaaagcata tagactggaa atcccgacca atgcaatatt taaactgtac gctaacgttt
    10261 ttgcagtagc aactgccact cgtaaacctg tgtaagatcc agggcctttt gcaaccacta
    10321 ttctttctaa atcttgaggt ttaagatcag tacaagtcat caaaaaatca atagcaggca
    10381 taaggctaac actatgattt ttctgaatgt taagggtcat atctgctaga agtgtctcat
    10441 catcaagtat agcaagggac aaggttttat ttgaggtatc aaatgcaagt gtcttcattt
    10501 tatgcctttc tactgcaagt tctagtatca attataacac aaattaagaa tcataaacaa
    10561 aaaggctgac tcttaatttg atttagacta taccataaat gtacctaaat tttttactaa
    10621 aagctaagtt tatggtataa ttataataat tgcacagaat caggaattaa tctactacac
    10681 aataatgaca acatataaaa ccgtcaaaat ggaacaccta ccttttgacc tatttattat
    10741 tgtttggata attgattcat aacgaaagga ataaaagaca aatgatttat aaagttttct
    10801 atcaagaaac aaaggatcaa agcccacgtc gtgaaagtac taaagcactc tatcttaata
    10861 ttgatgctac tgatgaactc gatggccgta tcaaggctcg tcgtctcgtt gaagataaca
    10921 cctattataa cgtggaattt atcgagctac tttctgacaa acacctcgat tacgaaaaag
    10981 agaccggtgt ttttgaatta acggagttct aagatgacaa atatcagttt aaaacctaat
    11041 gaagttgggg tgtttgccat tggtggtctg ggagagattg ggaagaatac ttatggtatt
    11101 gagtatcaag acgagatcat tatcgttgat gccggtatca aattcccaga agatgatctt
    11161 ttgggaattg attacgttat tccagactat tcttacattg tcgataactt agaccgggta
    11221 aaagctcttg ttattacaca tggtcacgaa gaccacattg gtggtattcc atttcttctc
    11281 aaacaggcta atatccctat ctacgcagga cctttagcac ttgctcttat ccgtggtaaa
    11341 ttggaagaac atggtctttg gcgtgaagca actgtttacg aaatcaatca caacaccgag
    11401 ttaactttta aaaacatgag cgtcactttc ttcaagacta ctcattccat tccagaaccg
    11461 gtcggtattg ttatccacac ccctcaaggt aagattatct gtactggtga ttttaaattt
    11521 gacttcacgc cagtaggaga tcctgcagat ttacaacgta tggcagcgct tggtgaagaa
    11581 ggtgtccttt gcttattatc agactcaact aatgctgaaa tcccaacctt tactaactct
    11641 gaaaaagttg ttggccaatc cattttaaaa attatcgaag gtattcatgg tcgtattatt
    11701 tttgcttcct tcgcttcaaa catctaccgt ttgcaacagg ctgcagaagc tgctgttaaa
    11761 acgggacgta aaattgccgt ttttggtcgt tctatggaaa aagcaattgt taacggtatt
    11821 gaacttggct atattaaagt tccaaaaggt accttcattg agccaagcga acttaaaaat
    11881 cttcatgcta gtgaagtttt aattatgtgt acaggtagtc aaggtgagtc tatggcagca
    11941 cttgctcgta tcgctaatgg cactcatcgt caagtaactt tacagcctgg tgatactgtg
    12001 attttctcat caagtccaat ccctggtaat accacaagtg tcaataaatt aatcaatacc
    12061 atccaagaag ctggtgttga tgttattcat ggaaaagtca ataatattca tacttctggt
    12121 catggaggac agcaagaaca aaagctcatg cttagtttga ttaaaccaaa atatttcatg
    12181 ccagtacatg gtgagtatcg catgcaaaaa gttcatgcag gactggcgat ggatattggt
    12241 attccaaaag aaaatatctt tattatggaa aatggagacg ttctagcttt aaccagtgac
    12301 agtgcgcgta ttgctggtca ctttaatgcc caagacatct acgtggatgg taatggcatc
    12361 ggggatattg gagcagccgt tttacgggac cgccgtgatt tgtcagaaga tggtgttgtc
    12421 ttagctgttg caactgttga ttttaacaca caaatgattt tagctggacc agatattctg
    12481 agccgtggtt tcatttatat gcgtgagtca ggtgacttga tccgcgaaag ccaacgtgtg
    12541 ctctttaacg ctatccgtat tgctttaaaa aataaggatg ctagcatcca atcagttaac
    12601 ggtgctatcg tcaatgctct tcgtccattc ttgtatgaaa agactgagcg tgaaccaatt
    12661 ataattccga tggtcttaac acctgataag cactaaaatc aaataagact catttcttaa
    12721 tactatctgt ctatatcttt aacatatcag ttctagttca gataattatc aaactaaaaa
    12781 ccagtcctca tagaacatgg actggttttt agttttctat aatggaaaat caattggtgt
    12841 ccgatcagct aataatagct tgctagctca aatacaatag ctcatccaaa agacccactc
    12901 aacttgagca ggtcctgata actctaatag ttatgaatat aatggtcaat ttcccattga
    12961 gaaacaaaag ttgcatagga agaccattca attcgttttg cttctaagaa attagtgtag
    13021 atatggtatc ctagtgcctt ttgtaccaca tcatcttttt gaagagcttt taaggcatta
    13081 tgaagcgttg atggcaaatc aataatgcct gcttcatttc gttcttccat tgtcatggta
    13141 taaatgttag cttcaacggg ttctggagct tcaattttgt taatgatacc atctaatcca
    13201 gcttccaaga gaacagccaa ggctaaataa ggattagctg tcggatcaac cgaacgtaac
    13261 tccaaacgcg ttcccatacc acgtgatgct ggaacacgga taagcggtga acgattactt
    13321 ccagcccaag cgacataaac aggtgcctca taacccggaa ctaatcgttt gtaagaattc
    13381 actgtagggt tagtgatagc agtgtagtta taagcatgct tcattagtcc tcccaagaaa
    13441 taataagcat cttctgatag ctgcattcct cgcttatcag tttcatcata aaaagcatta
    13501 ttaccttggt tatcaaacaa agacatgtta cagtgcatcc ctgatccagc tattccaaat
    13561 tttggtttag ccataaaagt agcataaagt ccatgttcac gggcaatcgt ttttacaact
    13621 agcttaaaaa tttgaatatt atcacaagct ttcaaaacat ctgcatattt aaaatcaatc
    13681 tcatgttgac caacagccac ttcatgatga ctagcttcca cttcaaaacc cattttcgtt
    13741 aaaatattca caatttcacg gcgcgtgttg tctgctaagt caattggcgc taaatcaaaa
    13801 taaccaccat tatcgttaac ttcaagtgtc ggattacctt tatcatccat cttaaaaagg
    13861 aaaaattctg gttctggccc aagattaaat gatttgtagc cgatctcgtt catgtgtttc
    13921 agggctcttt ttaaatttcc tctaggatct cctgcaaaag gctttccttc tgctgtataa
    13981 atatcacaaa ttaaacctgc aactgctcca ttttcatctc cccagggaaa aacaatccaa
    14041 gtgtctaaat cggggtaaag gtacatatct gactcattga tccgtacaaa accttcgata
    14101 gatgaaccat caaacataac cttgttagac aatactttgt ctaactgttc tttcgttgca
    14161 ggaatctcca catttttcat aacgcccatg atatcagtga acatcaagcg aagaaacgtt
    14221 acattttttt ctttgacttc atgacgaatg tcagctactg tgattgccat taaataggtc
    14281 tcctttaaat tttatacaat ttatatctta aatacgaaaa ttaccaatat gctgtgaggg
    14341 agtagaaaaa ccgctttgtg tcagcatttc atcatgtaag atccgtctga catctgcatc
    14401 tgttagagct ttttgttttt gcattaactt accttgacgc tcgacatatt cgcgtttaat
    14461 agctgcaata tttaatccct cagacaaaaa atctttaatt tccaaaagtc ggtccatgtc
    14521 atttaaggaa aacatacgcc gattcccttg cgttcgttca ggcttaatta aaccttgatc
    14581 ttcataatag cgaatttgtc tcgcagagag atctgttaat gtcatcaccg ttccaatagg
    14641 aaaaacagcc attgagcgtc taagttcttt ttctttcatg acaaactcct ttcatcttcc
    14701 ttattatagg ctgaaaaaaa taacctgtca agtatatatg ttatattatc taacatcact
    14761 gtgttttttg ttaaaccatt tatttttaat aagttcaaca tcagtattaa ctaaaatagc
    14821 tacaaagacg ccacaaaatg tttcaaatac ccgcgaagtc acataaataa acgtttgtcc
    14881 tgttggaata gacagtgtaa taatcaaaag agcagccact gctccaataa tacctgattt
    14941 attattacat gcaacattaa ccatgataca taacatggta caaataggaa caattaataa
    15001 agtaacccaa aaagcttgat gaaatatctc attgaatgca aaaaatacta aggagagtag
    15061 gccacctata ctattaccaa taattctaga aaagccaaag tgtacgcttt tatcaaagtc
    15121 ttctcttaaa ctaaatacag cagttagagc accaatttgg agccctttcc aaccaaataa
    15181 atgaaaaacc aataaaacta aaaagaccga taagcctgtt ttcaatgttc tcattcccaa
    15241 tttaaacttt ttaatatcaa acttgtaatt agataataat tttcttaacg ttttcatacc
    15301 tatcccttca ctatctattt taacatatca atccataaaa cccttttggt aaacgtttta
    15361 tttatgatta ggatatcttt tataaaccaa ttgatatgaa atcgtcataa acttttgaaa
    15421 attgcagtgt cagccaaata ccactatatc taaaaactag taaatgataa gtgaagttag
    15481 ggaaagttca ataactgtac atgatcaact aaaaaaagac atgcatcagc atgcccttta
    15541 ctatatatta taatgactat tatttttcag tcaatgctgc caaacctggt aatactttac
    15601 cctcgagaag ttccatgcta gcgcctccgc cggtactgat ccatgagaat ttgtcagcac
    15661 ggccaaggtt gatagcagct gctgctgaat caccaccacc gatgattgat ttaacgcctg
    15721 gttgtttaac gatagcgtcc atgacaccga ttgtaccagc ttggaagtca gggttttcaa
    15781 agacacccat aggtccgttc caaacaactg ttttagcacc agtaagtgct tcatcaaatt
    15841 tagcgattga tttaggaccg atgtcaagac caaggaagcc ttctgaaact gcttcacctt
    15901 cagtgtcgcg aacttcagtg taaccagcaa atgcgtttgc ttctttcgag tcaactggca
    15961 agatcaattt accatttgat ttttcaagga ggtctttagc aacatccaat ttgtcttctt
    16021 ctacaagtga gttaccgatt tcgatacctt gagctttgta gaatgtgtaa gtcataccac
    16081 caccgataag aactttatca gctttttcaa gaagattttc gataacacca atcttatcag
    16141 aaacttttga gccaccaaga atagctacga atggacgttc tggagtttca actgcttctt
    16201 ggatataagc aatttcgttt tcaagaagga aaccagctac agctttttca acgtttgctg
    16261 aaatacctac gtttgatgcg tgagcacggt gtgctgtacc aaatgcatcg ttaacgaaga
    16321 ttccatctcc aagtgaagcc cagtatttac caagttcttc gtcattctta gattctttct
    16381 taccgtcaac atcttcaaaa cgagtgtttt caaccaaaag aacttgtcca tcttccaaag
    16441 cattgattgc ttcttctaat tttgaaccac gagtaacacc tgggaataca acatcttgac
    16501 caagtttagc agctaaatca gcagctaccg gtgcaagtga ttttccttct ttgtcagctt
    16561 cttctttaac acgtccaagg tgagagaaga ggatagcacg accaccttgt tcgatgatat
    16621 acttgattgt tggaagagcc gcagtgatac ggttgtcgtt agtgataacg ccgtctttca
    16681 aaggcacatt aaagtcaaca cgaacgagga cttttttacc tttcaaatca acgtctttaa
    16741 cagtcaattt agccatgtga atagactcct taatattttt tatactcc
//
LOCUS       DAUELZ010000034        16431 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.034, whole genome shotgun sequence.
ACCESSION   DAUELZ010000034 DAUELZ010000000
VERSION     DAUELZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16431
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.034"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            181..831
                     /locus_tag="VHI09_000847"
     CDS             181..831
                     /locus_tag="VHI09_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEQ6120735.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITTDYDAYKQAHKPPYQKLLITEVNSCLEQLKKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSQKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            1076..1168
                     /locus_tag="VHI09_000848"
     CDS             1076..1168
                     /locus_tag="VHI09_000848"
                     /inference="COORDINATES: protein motif:HMM:NF018571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120736.1"
                     /translation="MAGDMFNRGHYLDLFLDWLVSKYRRIIILL"
     gene            1522..2646
                     /gene="queG"
                     /locus_tag="VHI09_000849"
     CDS             1522..2646
                     /gene="queG"
                     /locus_tag="VHI09_000849"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEQ6120737.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRISPESLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDTEFRKKIKTVIYGCDICQICCPYNKGINNSPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEMLAFMSHLTLKDEDSRKELELIRRKWQV"
     gene            2715..3813
                     /gene="prfB"
                     /locus_tag="VHI09_000850"
     CDS             join(2715..2786,2788..3813)
                     /gene="prfB"
                     /locus_tag="VHI09_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEQ6120738.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            3832..4524
                     /gene="ftsE"
                     /locus_tag="VHI09_000851"
     CDS             3832..4524
                     /gene="ftsE"
                     /locus_tag="VHI09_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEQ6120739.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            4517..5446
                     /locus_tag="VHI09_000852"
     CDS             4517..5446
                     /locus_tag="VHI09_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6120740.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(5756..6391)
                     /locus_tag="VHI09_000853"
     CDS             complement(5756..6391)
                     /locus_tag="VHI09_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ6120741.1"
                     /translation="MKIYKTINHIAGENTYYLVNDQAVILIDPGSNGQEIIAKIKSFE
                     KPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKEAAWLSSPDDNLSGLGRHDDII
                     TVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGVSFVFHSDELVVTGDALFRETI
                     GRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPSTTICHEKNANPFFH"
     gene            6623..7387
                     /locus_tag="VHI09_000854"
     CDS             6623..7387
                     /locus_tag="VHI09_000854"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEQ6120742.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGILDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMDQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            7495..9996
                     /locus_tag="VHI09_000855"
     CDS             7495..9996
                     /locus_tag="VHI09_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEQ6120743.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLGHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLVYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGSKALKSYQISKSFPINIALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRVQDDKDFAR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRV
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRQDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            10332..11525
                     /locus_tag="VHI09_000856"
     CDS             10332..11525
                     /locus_tag="VHI09_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ6120744.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            11546..12892
                     /gene="asnS"
                     /locus_tag="VHI09_000857"
     CDS             11546..12892
                     /gene="asnS"
                     /locus_tag="VHI09_000857"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEQ6120745.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGHEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDTLVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            13306..14196
                     /gene="rapZ"
                     /locus_tag="VHI09_000858"
     CDS             13306..14196
                     /gene="rapZ"
                     /locus_tag="VHI09_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEQ6120746.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYQVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            14193..15170
                     /locus_tag="VHI09_000859"
     CDS             14193..15170
                     /locus_tag="VHI09_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEQ6120747.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLAPPGDLRNVLLAMSDMPKFYERVFQYRFNESDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYQGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            15167..16078
                     /gene="whiA"
                     /locus_tag="VHI09_000860"
     CDS             15167..16078
                     /gene="whiA"
                     /locus_tag="VHI09_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEQ6120748.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSLIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYVQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
BASE COUNT         5164 a         2790 c         3443 g         5034 t
ORIGIN      
        1 atttaacaag atgagcctgt catttgatat ataggatata aactttttat gatcaaacaa
       61 aagtaatacc ttttgaattc gttagtattg acctgatcta aagtgatatc acttagaatg
      121 gactagctgt acagctttta cagcaactta tgctaaaata gaaaaaaagg agaaatgttg
      181 atgattaaag gaattatttt tgatatggac ggtgttttat ttgatacaga acctttttat
      241 ctgaggcgac gagaagattt ttttaagaca aagggaattc ccattgatca tttgaactct
      301 aaagatttta ttgggggcaa tctccaagaa ttatggaaag agttgttagg taaaaatagg
      361 gatgatgcta tcgttaaggc aattacaact gactatgacg cttacaaaca agcgcataag
      421 cctccttatc aaaaactgtt gattacagaa gtgaactctt gtcttgaaca gttgaaaaaa
      481 caaggtatca aactggctgt ggcatcaaac tcgaagcgtc aggatgtttt gttggcgttg
      541 gagacaacgc aaataaaaga ttattttgaa ataatcctag cgcgtgaaga tgtttctaga
      601 ggcaaacctt atccagatat ttataataaa gcagtacaaa aactaggatt acaaaaaaaa
      661 caactgcttg tagtagaaga cagccaaaaa ggcattgctg ccgccaaagc agccaatctg
      721 acagtttttg ccattaccga ctaccgatat ggcattgatc agagtcaagc tgatcacaag
      781 atagatcatt taggacaact gtgtgtaaaa atcggttgtt ttggatcgtg aagtaacgca
      841 actaactgat catgatactt caagacactt aagaatacgt ctaggacttt ctacagacca
      901 ataacaagaa tacgtttatg tgtttttact gttttttagc tacctattta caatatgaca
      961 ctgaaaagag tactatgttt aaattattat caaaaaaccg tcgcataaga tcacctagaa
     1021 aaggattatg atttttgtga tatggatgtg atcattagtc gttgaccatc ttcatgtggc
     1081 aggagatatg tttaaccgtg gtcattattt ggatttgttt ttagattggc tagtgagtaa
     1141 atatagaagg atcataatac tgctttgaca aaggcagtat ctgagtccat gagttgcacg
     1201 ggtaatgtga ctcggttttg gttaggtata ataatatttg gaagtgttat gctgcttgtc
     1261 ttcgtaggtc cattgcttat agtcagactt actttagagc tgagctaact gtctagacaa
     1321 gacgtgatgg agatgtgatt tctgaccaag tagaggtaac cagaaaaggg acttttacga
     1381 tgtaattgaa tttacttgtt gcattcttgt gcatgttaag agagaaaacc cagataaatc
     1441 aggaagtaaa atggttatgt ttgattagaa ttgtccttct ttttccttga agtcttaact
     1501 tattttgatt atgatagtag tatgactatt aaggcagaaa ttaaggcgct cgccaaagaa
     1561 attggtatct ctaaaattgg ttttacgacg gcagataact ttgattattt ggaaaagtca
     1621 ttgcgtgctt ctgtggaaga ggggcgcaat tcaggatttg aacataaagt catagaggat
     1681 cgtatctccc ctgaaagtct tttagaatcg gctaaaacga ttatttcaat tggcgttgct
     1741 tatcctcaca aacttcctca gcaacctcaa aaaacacctt ataaacgtgg aaaaattaca
     1801 cctagttcgt gggggcttga ttatcattat gtggtcggcg aaaagttaga cagactatca
     1861 aaaggcattg aagagctgtg tcgagatttt cctttgcaac aaaaagcaat ggtcgatact
     1921 ggagctttag ttgatactgc agttgcccaa agagcgggta ttggttttat aggaaaaaat
     1981 ggtctagtta tttctaaaga atatggctct tatatgtttt tgggagaatt aattaccaat
     2041 ttagagatcg agccagacaa gcctgtcgat tatgattgtg gtgactgtcg tcgttgtcta
     2101 gatgcctgtc caacttcttg tttaattggt gatggctcaa tgaatgccaa gcgttgtcta
     2161 tcatttcaaa cccaagataa gggaatgatg gacacagagt ttcgaaaaaa gattaaaaca
     2221 gttatctatg gttgtgatat ttgccaaatt tgctgtcctt ataacaaggg tataaacaat
     2281 tcaccagcta cagagattga tcctgagttg gctcaaccag aattaatacc atttttgagt
     2341 ttatcaaatg gacaatttaa agaaaaattt ggtatgattg ctggaagttg gcgaggtaaa
     2401 aatattttac agcgtaatgc cattattgcg ctagcaaatg ctcatgataa aaccgcagtg
     2461 gtcaaattaa tagagattat tgataaaaat aataacccta ttcatactgc aacagctata
     2521 tgggcattgg gagaaattgt caaaaaacca aacgatgaga tgctggcgtt tatgagtcat
     2581 ttaaccttaa aagatgaaga tagccgaaaa gagttggaat taatccgtcg taaatggcaa
     2641 gtttaagcct tttatggtat ttttaagaac cttatgataa aataaaacag attaacaaag
     2701 attggagaaa tctcatggaa gtggctgaaa ttcgccaaaa aatagtagaa aataaagaga
     2761 agttgactag cttcaggagg tctctttgac ttagatcgtt tggaagagga gattgctctt
     2821 cttgaaaatc acatgactga gccagacttt tggaatgaca atattgcagc gcaaaagaca
     2881 tctcaagaac taaatgagtt aaaaggtaag tatgatactt tccataatat gcaagaatta
     2941 tctgacgaaa cagaactctt acttgagatg cttgacgaag atgattcttt gaaagaagaa
     3001 ttagaagaaa atctaatgca gctagataag ataatgggtg cttatgaaat gacgcttctc
     3061 ttatctgaac cctatgacca caataatgct atcttagaaa tccatcctgg ttcaggagga
     3121 actgaagcgc aagattgggg agatttactc ttacgaatgt atacccgttt tggtaatgct
     3181 aatggtttca aagtagaagt attggattat caagcaggag atgaagctgg tattaaatcg
     3241 gttaccctct cttttgaagg ccccaatgct tacggtttgt tgaaatcaga aatgggagtt
     3301 catcgcctcg ttcgtatatc accttttgat tcagctaaac gtcgtcatac ttcatttgct
     3361 tcagttgaag tgatgccaga gttagatgat acgattgaag tagaagtacg tgatgatgac
     3421 attaaaatgg atactttccg ttccggagga gctggtggtc agaacgtcaa taaagtatca
     3481 acaggtgttc gtttgacgca tattccaact ggaattgtgg tatcgtctac cgttgaccgt
     3541 actcaatatg gaaaccgcga tcgcgctatg aagatgttgc aggctaagct atatcagtta
     3601 gaacaagaaa agaaagcaca agaagtagat gctctcaagg gtgataaaaa agaaatcact
     3661 tggggaagtc agattcgttc ttatgttttc accccttata cgatggtgaa ggatcatcgt
     3721 actaactttg agcttgctca ggtagataag gttatggatg gagaaatcaa tggctttatt
     3781 gatgcctacc ttaagtggcg cattgaagac taaaaataat aaaggagaaa catggcacta
     3841 atagaaatgt ctggtgttac taaaaaatac cgccgctcaa cgaccgccct cagagacgtc
     3901 aatgtttctg ttaaccaagg tgagtttgtt tatctcgtag gcccatcagg tgctggtaaa
     3961 tcaactttta tcaaactcct ttaccgtgaa gaacaactga caactggtaa gctatatgtg
     4021 ggtgaattca atttaactaa gctaaaagca cgtgatgttc cgattcttcg tcgtcacatc
     4081 ggagtagttt ttcaagatta caaactgcta ccaagaaaaa cagtctttga aaatgtagct
     4141 tacgccatgg aagttattgg tgaaaagcgc cgccacatta aaaaacgcgt gccagaggta
     4201 ctcgatttag ttggcttgaa acataagatg cgttcttttc caagtcagct atcaggaggg
     4261 gagcagcaac gtgtggccat tgcgcgtgcg attgttaaca accctaaatt attgattgca
     4321 gatgaaccga cagggaattt agatcctgag atttcatggg aaattatgca gttacttgaa
     4381 cgtattaatg ttcagggaac aactatctta atggcgacac acaacagtca tattgtgaat
     4441 acatttcgtc atcgtgttgt tgctattgaa gatggtcgca tagtacgtga tgaagaaaaa
     4501 ggagattatg gttacgatga ttagatactt tttccgtcat atttgggaat ccattaaaaa
     4561 tcttaaacgc aacttttgga tgacatttgc ttcagttagt atggtggctg ttacattgac
     4621 tttagttggt gtttttgcgg cgacattgtt gaatatacag cgtgttgctt cgggtgttga
     4681 aaataatgtc catatcaata cctacttaca agttgattca actgatgctg caaaagttat
     4741 tcaaaatact gccggtgaac ctgttaataa cgataactac cacagtgttt acgacaaaat
     4801 tgctcagata aaaggtgtca aaaagattac attttctagc aaggatgaac aattgaaaaa
     4861 attgcaagaa accttaggtg atgtttggaa tatgtatgac caagacacta atccgctcca
     4921 agatatctat cttattgaga cccagacgcc taaacaagtt aaggcgatca ctaaaaagat
     4981 cagaacgatt gaaggcgtag aagccgcaga ttatggaggg attaactccg ataaactatt
     5041 taagttttca acattaattc aaacttgggg gttgatcgga acagcaatgc tattatttgt
     5101 agctgtcttt ctcatttcaa acactattcg tatgactatt atgtctcgta aacgcgacat
     5161 tgaaattatg cggttagttg gtgctaaaaa ttcttatatt cgagggcctt tcttttttga
     5221 aggggcttgg gttggcttgt taggagctgt tttaccatct cttctgattt attatggata
     5281 tgatcttgtt tataagcatt ttgcacaaga attacaacgt aataatctct cgatgtatcc
     5341 gcttgatcct tatgtatatt acctgattgg tgctttgttt gtcataggta ttatgattgg
     5401 ttcgcttggt tcagtgcttt ctatgagacg ttatttaaaa ttctaaaaaa agcgctgctg
     5461 acgaaacgta gcgaccccca aaagttggac ctcttatgtt attccaagga cttcgttttg
     5521 tattgcacag gactggtcct tttcattttg ttttgattca tttgtttatt tgtaataggc
     5581 aatatagttt aaaatagcag tttctaaggt ttcaagagaa gaaaagcacc ccaaaagtta
     5641 gatgtttgat gtatctaact tttggggtgt cattcaaaat ttagtggctc ttttttagta
     5701 aaatactatg atttcagttt taggataacc tcaaacagta tctaaaatag ctatgtcaat
     5761 gaaaaaaggg gttagcattt ttttcgtgac aaatggtagt agaaggacca tgcccaggat
     5821 aaactcggta atgattaggt aaggtgaata gttcttggcg aataccagta atgaggtcct
     5881 caaagttact ggtaggtaag tcagtacgac cgatagtttc acgaaataga gcatcgccag
     5941 taacgactaa ttcatctgag tggaaaacaa atgagactcc tccccaagaa tgaccaggtg
     6001 ttggtaaaac tgtgaattca aaaccattta gttgataggg ttgctttaat ttaaaaaagt
     6061 tctctgccgg tcttgctata acagtaatga tatcatcatg acggcctaag ccagataaat
     6121 tatcatctgg ggaagatagc caagcggctt ctttttctga aacataaaca ggtggatgat
     6181 caaaagcatc tctgaccaaa tctaaactaa aaatatgatc atagtgagta tgcgttaaga
     6241 ggatagccac taaaggtttt tcaaaggatt taattttagc aatgatttct tggccgttac
     6301 ttcctggatc aatgaggata acagcttgat cattaacgag gtaataagta ttttcaccag
     6361 caatgtggtt tattgtttta tagattttca taaggatagt gtaccaaaaa atcaaggctt
     6421 tgttaaaaaa aagagtggcg gaaaataaat ggcaattagt ttcagaaaat atgagatctc
     6481 acagcaattt attgacaccg ttttcataaa ggtgtatcat aaaggtaatc aataagccaa
     6541 gcctaaagct gaacttagtt taagaacact gactttttga gagcaaccta atcggttgac
     6601 taaaaaatat gaggtggaag atatgtcaaa agttgctatt gttactggtg ctggtcaagg
     6661 aattggattt gctattgcga aacgtcttca tgcagacggc tttaaaatag gcattttaga
     6721 ctataatgaa gagactgctc aggctgcggt tgataaacta tcacctgagg atgctgttgc
     6781 agttgtagca gatgtttcaa aacgtgacca agtttttgat gctttccaaa aagttgtaga
     6841 tacttttggt gatttgaatg ttgttgttaa caatgcaggt gttgccccaa caacccctct
     6901 tgacacgatt actgaagagc agtttgaaaa ggcttttgca atcaatgtcg gaggaacgat
     6961 ttggggaagc caagcagctc aaaaacattt tagagaatta ggtcatggcg gaaaaattat
     7021 aaatgcaaca tcacaagcag gatgtgaagg gaatcctaat ttgactgttt atggtggtac
     7081 aaaatttgca gttcgtggta ttacacagac tttggcaaag gatttggcat cagaaggaat
     7141 cacagtcaat gcttacgcac caggtattgt taaaacacct atgatgtttg atattgccca
     7201 tgaagttggt aaaaatgcag gcaaagatga tgaatggggt atggatcaat ttgcaaaaga
     7261 tatcactttg aaacgcttgt ctgaaccaga agatgttgct aatgcagtag gcttcctagc
     7321 gggagatgat tcaaattata tcactggaca aacaatagtt gtcgatggtg gtatggtatt
     7381 ccattaaaaa tgattaatac tgacactgtg agtgaaactc acagtgtttt ttatacttct
     7441 taaaaaatta tcctatcttg gttattggag tgatggcacc tttgagatat agtcatgttt
     7501 tgttttatag atatagcgtg ctataataga cttactatga cccagaaaaa acttagaaaa
     7561 tatgcagtag ttgacttgga ggctactgga gcaggtccca atgcttctat tattcaggtt
     7621 gggattgtta ttatccaagg aaataaaatt attgatagtt atgaaacaga tgttaatcct
     7681 catgaatctt tagatgagca tattgttcat ttgacaggta ttacggataa gcagttggct
     7741 aaagctccgg attttggtca agtggcgcat cacatttatc aattgattga agattgtatt
     7801 tttgttgccc acaatgtaaa gttcgatgca aatttattgg cagaagctct ttttttggaa
     7861 ggatatgagt taacaatacc acgtgttgat actgttgaat tagcccaact atttttccct
     7921 cgctttgaaa agtataacct tggtcaccta tctaggcagc tcaatattga tttggcagaa
     7981 gctcatactg ctattgcaga tgcaagagct acagctatac tgtttttaag acttttacaa
     8041 aaaattgaga gccttccaat tgagtgctta gaaagcttat tagtctattc agatagcctg
     8101 ctatttgaaa cagcaatggt cattcaagaa ggattagcca aagccaaacc ttatgatccc
     8161 aacaaataca ttaaaatcag acagattctc ttaccaaagg gctcgaaagc tctcaagtct
     8221 tatcaaatat caaaaagctt ccctattaat atagcactat tgggtctgga agaaaggcct
     8281 aaacagactc aatttgctca gttaattgac gaagattacc atcagggagt agctagtttt
     8341 attgaagctc agacaggtat tggaaaaacc tacggctacc tgctacccct tttggcaaaa
     8401 gaagatcaaa accaaatcat tgtcagtgtg cccaccaaac ttcttcaaga ccaattaatg
     8461 gctggtgaag tagctgctat tcaagaacaa tttcacatcg cctgccatag tttaaaaggg
     8521 cccgccaatt acctaaagct agatagcttt gcagatagtt tggaccaaaa tgaccagaac
     8581 cgtttggtga atcgttataa aatgcaatta ttggtttggc tcttagaaac taagacagga
     8641 gaccttgacg aaatcaaaca aaagcagcgc tttgcagctt attttgagca gcttaagcac
     8701 gatggagaca ttaagcagtc ttctgaattt tacgattacg atttttggag agttagttat
     8761 gaaaaagcca aaacagcccg tctcctgatt accaaccatg cttatttttt acaccgtgtt
     8821 caagatgaca aggactttgc tagaaacaag gtcttggtct ttgatgaggc ccaaaaactg
     8881 atgctccaat tagatcaatt atcacgtcat cagcttaacc tgacggtttt tttacaaaca
     8941 atacaggcca agttaagcaa ccctttacct cttttagaaa aacgcttatt ggaaagcttg
     9001 tcgtttgaac tgggacaagt gtcaagtgat tactatcaaa ataaagagca ccagcttgct
     9061 catgattggt ctcgtatagc tggctacgcc aaagaattaa caggagcaga ttatcaagag
     9121 ttacaagcct tttttgctac ctcagatggt gactactggc ttagtagcga aaagcaagag
     9181 gagaagcggg tgacttatct aaacagtgct agtaaggcat ttattcattt ccaacagctc
     9241 ttaccagaaa cggtgaaaac ttattttgtt tctgccactt taaccatcag ctcagaagta
     9301 accttggcag acctattagg ttttgaagag tatctctatc atgtcattga aaaggataaa
     9361 aagcaagacc agttggtgct tgttgatcaa gaggcgccta tcgtgacaga agtcagtgac
     9421 caaatctatg tggaagcaat tgcaaaacga atcgaaagct tgaagcaaga gggttatcct
     9481 attttagtgc tttttaattc taaaaaacac ttgctgttgg tttcagacta tctagaccaa
     9541 tggcaggtgc ctcacttagc gcaggaaaag aatggaacag cttataatat taaaaaacgg
     9601 tttgatcaag gagagcaaac tattttgctt ggtttaggtt ctttttggga aggagttgat
     9661 tttattcaag cggatcgtat gattaccctt atcgcacgat tgccctttga taacccagag
     9721 gatttctttg ttaagaaaat gagtcattat ctattggaaa aaggtaaaaa tcctttcaga
     9781 gattacttct tgccaatgac tattttgcga ttaaaacagg ctattggtcg cacgatgcgt
     9841 cgacaagatc aaaagtcagt ggttattatt ttggatcgtc gtctccttac aaagtcctat
     9901 ggacaagtta ttttggaagg tctcggtcag gaatttttga tttctcaaca aaattttcac
     9961 gattgtctag tagaaacgga ctgttttctg atataatgga aaggatctat tatatcagaa
    10021 aaatttttaa tggcgttaag cacaatcaac taacatttaa attagtttga acttttatta
    10081 tgctctttta gacaactgtt caaatgtttt cgaattgttt tttttgataa ggacaataaa
    10141 tgaacaaatt tttggtttta gtaaacccaa ttactgttct tttcattagt gtcagttgct
    10201 atcaaaacag cttgtcatag ttcgtgcgat cagtagcgag tattggtaaa actgaaaccg
    10261 tagattatga aaaaatgatc tgttgatcat taattaatct gaagataaga agtcgataaa
    10321 aaggagatac gatgcccaaa ctatcaaagc gagttttaga aatgaaagag agtgttactc
    10381 tggctgcagg tgctagagct aaggctttga aagcgcaggg ccgtgatgta ctgaatttaa
    10441 ctttaggaga accagatttt tttacaccta agcacattca agacaaggcg attgaatcta
    10501 ttcaaaatgg tacggctagc ttttatacaa atgcttctgg gcttccagaa ttaaaagctg
    10561 cgattgccac atacctgaaa aatcagtatg ggtatcactt gtctcctgat cagattgtgg
    10621 caggaacagg tgccaagttc atcctttatg ccttttttat ggctgtttta aatcctggtg
    10681 accaagttct cattccaaca ccatattggg tatcttattc tgaccaagta aaaatggcag
    10741 aaggtcagcc gatcttcgtt caaggactag aagaaaatca attcaaggta actgttgacc
    10801 agctggaaag agcaaggaca agtaagacta aagttgtcct tatcaattcc ccttccaatc
    10861 caacaggtat gatttatggt gctgaggaat taagggccat tggagaatgg gcggtgcaca
    10921 acgatattct tattctggca gatgatattt acgggtcctt agtctataac ggcaatcagt
    10981 ttgttcctat ttcaacactc tcagaagcga ttcgtcgtca aaccattacg gtcaatggcg
    11041 tggctaaaag ttatgccatg acaggctggc gggttggttt tgcagcaggt gaaccagaga
    11101 tcatttcggc aatgagtaag atcatcgggc agactacctc taacctgaca accgtttcac
    11161 aatatgctgc tatcgaggct ttctgtggta gtcagtcatc acttgaagag atgcgattag
    11221 cgtttgaaga gcgccttaat atcatttatc cattgttgtg tcaggtccct gggtttgagg
    11281 ttgttaaacc acaaggagct ttttacttct ttccaaacgt taagaaagcc atggaaatga
    11341 caggtttttc cgatgtgaca agctttgcta atgctatttt agaggaagtc ggattagctg
    11401 ttgtgagtgg agcaggcttt ggcgcaccgg aaaatgttcg tctgagttat gctacagata
    11461 tcgaaaccct taaagaagct gttcgtagac tgcatgtctt tatggaaagt aatgaaatta
    11521 attaaataaa aatagagaga aacttatgtc aaaaaaactt atttcaattg ttgatgttaa
    11581 agattatgtt ggtcaagaag ttactattgg tgcttgggtt gctaacaaat caggtaaagg
    11641 taagattgcg tttgtacagt tacgcgatgg ttctgctttt ttccaaggtg tcgcttttaa
    11701 accaaacttc attgaaaaat atggtgaaga atcaggtcat gaaaaatttg acgttattaa
    11761 acgtctcaac caagaaacat ctgtttatgt aacagggatt gttaaagagg atgagcgttc
    11821 taaatttggc tatgagcttg atattactga ccttgaaatc attggagagt cacatgaata
    11881 tcctataact cctaaagagc atgggacaga cttcttgatg gacaaccgtc atctttggtt
    11941 gcgttctcgt aaacaaatgg ctgttatgca aattcgtaac gctattattt atgcaactta
    12001 tgaattcttt gaccaaaatg gttttatcaa atttgatagc ccaattttat cagaaaatgc
    12061 agcagaagat tcaacagagt tattcgaaac agactatttt gggaaaccag catttcttag
    12121 ccaatctggt caattatacc ttgaagctgg agcaatggct ctaggccgag ttttcgactt
    12181 tggtccagtc tttcgtgctg aaaaatctaa aacacgtcgc catttaacag aattttggat
    12241 gatggatgct gagtattctt tcttgtcaca tgaggaatca cttgaccttc aagaagctta
    12301 tgttaaagcg ttgatccaag gcgttctaga tcgtgcgcct caagcacttg atattttaga
    12361 acgtgatgtt gaggctctca aacgttatat cacagagcca ttcaagcgtg tttcttatga
    12421 tgatgctatt acacttcttc aagagcatga agcagatgaa gatacagatt atgaacattt
    12481 agagcatggt gatgactttg gttccccaca tgaaacttgg atttctaatt actttggagt
    12541 accgacattt gtagtgaatt acccagcaag ctttaaagca ttttatatga aaccagttcc
    12601 tggaaatcca gaacgcgttc tttgtgctga tcttttagct ccagaaggtt atggagagat
    12661 cattggcggc tcaatgcgtg aagacaacta cgatactctt gtggctaaaa tggatgaact
    12721 aggtatggat aaatcagaat atgactttta ccttgacctt cgtaaatatg gctctgttcc
    12781 tcatggcggt tttggtatag gtattgaacg tatggtaact tttgttgctg gtaccaaaca
    12841 tattcgcgaa gctattccat tcccacgtat gttacatcgg attagaccct aataatacaa
    12901 aaagttcatg tcatctatga actttttgta ttggtaagaa gtgagtgtgt tataaaaaaa
    12961 tcaatatgtc ctaaaggaga gataagaaat agtagagttt gaacctacat cctttgtgaa
    13021 gataaatttt ggaagtttag tagatcagtt agcagataag acaatatagc cttgctcaag
    13081 ggatatttta tagacaagta tttggactac gattatgaaa tagacactct catttgatac
    13141 taaaaaggtc tcgtctattc ctataatagg agagctacgg gagttattat taaaattagc
    13201 tgatttttac agttcaaaat gtgttaccac tttttcactt cttaggtttc ttatgtatgt
    13261 ggtataatga aggtggacgg ttcgatagag aaagggaaat tgtaaatgtc agacaaacac
    13321 attaatttag ttattgtgac aggaatgagc ggcgctggaa aaacagttgc cattcagtct
    13381 tttgaggatc taggctactt taccattgat aatatgcccc cagccttggt tccaaaattt
    13441 ttagaattaa ttgaacaaac caatgaaaat cgtagggtgg ctttggttgt cgatatgaga
    13501 agtcgtttgt ttttcaagga aattaattct accttagata gtattgaaag caatcctagc
    13561 attgattttc ggattctttt tttggatgca acagatggag aattggtgtc acgctataaa
    13621 gaaaccagac ggagccaccc tttggctgcg gacggtcgtg tgcttgatgg tattcgattg
    13681 gaaagagaac tcctatctcc tttgaaaagc atgagccaac atgttgtgga tacaacaaaa
    13741 ttgaccccta gacaattgcg taaaaccatt tcagaccagt tttctgaagg gtctaatcaa
    13801 gcctctttcc gtattgaagt gatgagcttt gggttcaaat atggtcttcc tttggatgcg
    13861 gatttggttt ttgatgtgcg ttttctaccc aatccttatt atcaggtaga gcttcgtgaa
    13921 aaaacaggac tagatgagga cgtttttaat tatgtgatgt ctcacccaga atcagaggtg
    13981 ttttacaagc atttgttaaa ccttattgtc cctatcttac cggcttacca aaaagaaggg
    14041 aagtctgtct tgacggtggc tattggctgc acaggaggcc aacaccgcag cgttgccttt
    14101 gcccattgct tggcagaaag tctggcaaca gattggtcgg ttaatgaaag ccatcgtgat
    14161 caaaatcgtc gtaaggaaac ggtgaatcgt tcatgaaaaa tcctaaaatg actgttatag
    14221 gtgggggtac tggcatctcc attatcctaa aaagccttcg taatgaagca gttgatatta
    14281 ctgctgtggt cactgttgca gatgatggtg gttcctcagg agaacttcgt aatgccatgc
    14341 aattagcccc gccaggagac ttacggaatg tcttgttagc gatgagtgac atgccaaaat
    14401 tttatgagcg ggttttccaa taccgtttta acgagagtga tggagccttg gctggtcacc
    14461 ctctaggcaa tctcattatt gctggtatct ctgaaatgca aggctcaacc tacaatgcca
    14521 ttcaaatttt aaccaaattt ttccatatta caggtaaaat ttacccctct agcgagcagg
    14581 ccttgaccct tcatgctgtt tttaaagatg ggcatgaagt agcaggagaa agttctattg
    14641 ccaaatacca aggcatgatt gaccatgtct atgtgaccaa cacctacaac gaccaaaagc
    14701 ctcaggccag tcgtaaggtg gttgaggcta ttctagaaag tgacatgatt gtcttgggac
    14761 caggctcact gtttacctct attttaccta atttggtcat tccagaaatt aaagaagctt
    14821 tgcgccagac taaagcagaa gtggtgtata tttgtaatat catgacccag tatggtgaaa
    14881 cagaacaatt ttcagatgca gaccatgtgg ctgttttaaa tcagcactta ggaagagatt
    14941 tgattgatac ggttttggta aatgtggcta aggtgccgca ggcttacatg aattctaaca
    15001 aatttgatga atacctcgtt caggttgatc acgactttgc tggcctttgc agggctgcta
    15061 agcgtgtgat ttcgtcttat tttttacggt tggagaatgg cggagctttt cacgatggta
    15121 acttagtggt ggaagaatta atgaatttag tgaggatagt caagcaatga gttttacaac
    15181 caaggtcaaa gaggaattaa ttcacttgtc cacaggtgac aacaatgaat tggccgccat
    15241 tatcaaatta tcaggcagct tgggcttagc tcaccaaagc cttcatttat ccattaccac
    15301 tgaaaatgct aaaattgccc gctacattta ttctcttatt gaagacgcct atgttatcgt
    15361 gccagaaatc aggtatcatc aaaagacgaa cctgcgcaaa aaccgtgttt acaccgtcta
    15421 tgttgagcaa ggtgtggaaa ccattttagc cgatttgaaa ttagcggact ccttttttgg
    15481 tctggaaaca ggcatcgaac ctcaggtttt atcagacgat aatgctggcc gttcttatct
    15541 taaaggggct tttctagcgg caggtagcat cagagatcct gagtcaggca agtatcagtt
    15601 agaaatctat tcagtttatc ttgatcatgc tcaagattta gctcaactca tgcaaaaatt
    15661 tatgctagat gccaaaacca tcgagcataa aagcggtgca gttacttatg tgcaaaaggc
    15721 agaagatatc atggactttt taattataat tggtgccatg tcctgcaaag aagactttga
    15781 agccatcaaa ttacttcgag aagctcgaaa tgatattaac cgtgccaata atgcagaaac
    15841 agctaatatt gccaaaacta tcagtgctag catgaaaact attaataata ttattaaaat
    15901 catggacaca attggtttag aaagtttacc gattgaattg caacaggtag cccagcttcg
    15961 ggttaagcat ccagactatt ccattcaaca agttgctgat gcccttgagt tcccaatcac
    16021 caagagcggg gtcaaccatc gcttgcgtaa aatcaataaa attgcagatg acttatagaa
    16081 catttttatc gtatgagcct ttgttcatac gatttttctg tctaaaagtc gttttccatt
    16141 gtttttttga agtgtctgat gttctgtgat atgataaaag ggataatatc gtgtgtaaag
    16201 gagactatta tgcgcaacaa taaaatacaa gattcatgta caactatact cgttgtagtg
    16261 cctaaataat gcttttaaaa cttaaaaata atatcgatgt tatccatagt aacctcgact
    16321 ctatctatca attgcttaac tatcctagat tgaacatcat acgaagattt tctaatgtca
    16381 atgttatcca tcagcattcc aagagagtct tcgtttttct tgtctaaata a
//
LOCUS       DAUELZ010000035        16351 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.035, whole genome shotgun sequence.
ACCESSION   DAUELZ010000035 DAUELZ010000000
VERSION     DAUELZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16351
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.035"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            14..667
                     /gene="grab"
                     /locus_tag="VHI09_000861"
     CDS             14..667
                     /gene="grab"
                     /locus_tag="VHI09_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G-related alpha2-macroglobulin-binding protein
                     GRAB"
                     /protein_id="HEQ6120749.1"
                     /translation="MGKEIKVKYFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQSRII
                     PNGGTLTNLLGNAPEKLALRNEERAIDELKKQAIEDKEATTAIEAASSDALEALADQA
                     DALQSEEAAVVQSDTAASDALEALADQADALQSEEAAVVKADTAASDAWEKAATPIAL
                     DVKKTKDTKPVVKKEERQNVNTLPTTGEESNPFFTAAALAIMVSIGALVVSSKCKEN"
     gene            905..1447
                     /locus_tag="VHI09_000862"
     CDS             905..1447
                     /locus_tag="VHI09_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ6120750.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            1456..2739
                     /locus_tag="VHI09_000863"
     CDS             1456..2739
                     /locus_tag="VHI09_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ6120751.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCEDSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMIYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2755..3615
                     /locus_tag="VHI09_000864"
     CDS             2755..3615
                     /locus_tag="VHI09_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEQ6120752.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTGSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            3617..4582
                     /locus_tag="VHI09_000865"
     CDS             3617..4582
                     /locus_tag="VHI09_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEQ6120753.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSGLSPSQSIEDSHTD"
     gene            4693..5976
                     /gene="aroA"
                     /locus_tag="VHI09_000866"
     CDS             4693..5976
                     /gene="aroA"
                     /locus_tag="VHI09_000866"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEQ6120754.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNHNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSRLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDTLNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            5969..6460
                     /locus_tag="VHI09_000867"
     CDS             5969..6460
                     /locus_tag="VHI09_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEQ6120755.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFTNDNSVIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            6668..8119
                     /locus_tag="VHI09_000868"
     CDS             6668..8119
                     /locus_tag="VHI09_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ6120756.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDTPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIF
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            8168..9559
                     /gene="rlmD"
                     /locus_tag="VHI09_000869"
     CDS             8168..9559
                     /gene="rlmD"
                     /locus_tag="VHI09_000869"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ6120757.1"
                     /translation="MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHGGFVFFVD
                     NALPEEVIDMRVLKVNKNSGFGKVEVYHYLSPARNADVNLTYLRTGIADLGHLTYEDQ
                     LTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
                     SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGE
                     MMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTI
                     TDSMLGNNYAISAQSFYQVNTIMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFA
                     KTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFIADTAEHAMATWAKDGIKPSVILVD
                     PPRKGLTESFIQASVAMGPQKITYVSCNPATMARDIKHYQELGYKLTKVQPVDLFPQT
                     HHVECVSLLEKRS"
     gene            9711..10489
                     /locus_tag="VHI09_000870"
                     /pseudo
     CDS             9711..10489
                     /locus_tag="VHI09_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            10574..10975
                     /locus_tag="VHI09_000871"
     CDS             10574..10975
                     /locus_tag="VHI09_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEQ6120758.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     DAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(11080..12564)
                     /locus_tag="VHI09_000872"
     CDS             complement(11080..12564)
                     /locus_tag="VHI09_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEQ6120759.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLEKNGIASYLHYPIKGYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            12938..14158
                     /locus_tag="VHI09_000873"
     CDS             12938..14158
                     /locus_tag="VHI09_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ6120760.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLGL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGIAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     TIGQLKAERYQQLR"
     gene            complement(14278..14643)
                     /locus_tag="VHI09_000874"
     CDS             complement(14278..14643)
                     /locus_tag="VHI09_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEQ6120761.1"
                     /translation="MTIYSHTYETHPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            14758..15489
                     /locus_tag="VHI09_000875"
     CDS             14758..15489
                     /locus_tag="VHI09_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ6120762.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKTVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVAGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKEVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
     gene            complement(15506..15868)
                     /locus_tag="VHI09_000876"
                     /pseudo
     CDS             complement(15506..15868)
                     /locus_tag="VHI09_000876"
                     /inference="COORDINATES: protein motif:HMM:NF024997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(15875..16228)
                     /locus_tag="VHI09_000877"
     CDS             complement(15875..16228)
                     /locus_tag="VHI09_000877"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ6120763.1"
                     /translation="MNSHHACQTLQVSRSGFYAYLKRRLSSKHVENEALKEMIKDIFY
                     EHKERYGSVRITQELCRRGIHVNHKRVGRLLHQLGLYAKGSRYQYKYYNRRRSSLTRP
                     NLVNQCFQATGKNKL"
BASE COUNT         5037 a         2874 c         3453 g         4987 t
ORIGIN      
        1 aaaaggagaa acaatgggaa aagaaataaa agtgaaatac tttttgcgta gatcagcttt
       61 tggattagtt gcggtgtcag catcagtatt agtcggttca acagtatctg ctgttgactc
      121 acctatcgaa cagtctcgaa ttattccaaa tggcggaacc ttaactaatc ttcttggcaa
      181 tgctccagaa aaactggcat tacgtaatga agaaagagcc attgatgaat taaaaaaaca
      241 agctattgag gataaagaag ctacgacagc tatagaagca gcaagttcag atgccttaga
      301 agcattagcg gatcaagcag acgctttaca atcagaagaa gctgcagtag ttcaatcaga
      361 taccgctgct agtgatgcct tagaagcatt agcggatcaa gcagacgctt tacaatcaga
      421 agaagctgcg gttgttaaag cggataccgc tgctagtgac gcctgggaaa aagcagcaac
      481 tccaatcgct ttagatgtta agaaaactaa agatacaaaa cctgtagtta aaaaagaaga
      541 aagacaaaac gttaataccc ttcctacaac tggtgaagag tctaacccat tctttacagc
      601 tgctgcgctt gcaataatgg taagtatagg tgcgttagtt gtaagttcaa agtgcaaaga
      661 aaattagttg ctatttgttc tagcaaatga aacaagggaa tcgaaagatt ctctttttct
      721 gatatttaaa aatgctacag tttataatag cttgaaagac taaatgttct caaactcatt
      781 agaacaagct ttaataaaac taacccagtt gtatcaaagg atagataaag actaagactg
      841 ttatagaata tttgcaaaag atttgttata atggacccaa tacaggtatt gtgaaaggta
      901 taatatggat atttggacaa agcttgcagt gtttgctttt tttgagactc cgaaagtaat
      961 attacggcct tttcgctatg aagatcattg ggatttttac agcatggtta acgacactaa
     1021 aaacctttat tatgtttttc cagaacaaaa aactaaggca gcaagtgact atcttttagt
     1081 acatagtttt ataaagtttc ctttaggtca gtgggcaata gaagataaag caacccacca
     1141 agtaataggt tctattagaa ttgagcatta tgatgctaaa acgcgttgtg ctgatattgg
     1201 ctatttttta aactatgcct tttggggaca aggaattatg acagaagtcg taataaaact
     1261 tgtttattta tcttttcacg aatttggtct caaaacgttg cgtattataa ctcatttaga
     1321 aaataaggcc agccagaaag tagctaaaaa agcaggtttt caactaaaaa cctgttttaa
     1381 aggaagtgat cgtaatactc ataaaatctg catttataaa atgtaccaac taactaatga
     1441 taggtgataa tcattatgag caaacatcaa gatattttag attatttaga aaaacttgct
     1501 attggtaaaa aagtgagtgt tagaagcatt tcaaaccact taaaagtaag cgatggaaca
     1561 gcctaccgtg ctattaaaga ggcagaaaat cgaggtattg tcgaaaccaa accaagaagt
     1621 ggaactgttc gtattgaaaa aaaaggacgg gttcgtattg accgtctgac ctattccgaa
     1681 attgctcgga tcagtgattc ggaagtgcta gcgggacatg ctggtttagg acatgaattt
     1741 agtaggtttt caattggtgc tatgactcaa caaaatattc gtcgttactt ggtcaaagga
     1801 gggttattaa tagttgggga tcgtgaaacg attcaactct tagctcttga aaaccataat
     1861 gccattttgg taacgggcgg atttccagta tccaaacgtg ttattgaaat ggcaaataac
     1921 caacgaatcc cagttatggt aactcattat gataccttta ctgtggcaac aatgattaat
     1981 cacgcgctat ctaatattcg tattaaaaca gatctcaaaa cagttgaaca ggtaatgata
     2041 cctattacag attatggtta cctttgcgaa gacagctctg ttgaagaatt caatacatta
     2101 ataaaaaaaa cacgacaagt caggtttcct gtattagatt ataaaagaaa agtgattggt
     2161 gtagttagta tgcgtgatgt ggtggatcag ttgccaacaa ctaagcttac caaggtcatg
     2221 tctaaaaatc caataacggc tagaccaaat actagtcttg ctaatattag ccagaaaatg
     2281 atctttgaag atttaaacat gttacctgtc actgacgaag aaaacaattt acttggtatg
     2341 attactcgta gacaagcaat ggaaaatttg ccaaatcacc aaccaaataa cccctatact
     2401 tatagtgagc aaatactttc taaccttgaa gaaacggttg attattacca agtagtagtt
     2461 gagccaacaa tgattgatag tgctggtaac atgtcaaacg gtgttatttc agagttctta
     2521 aaggaaatta gtatcagagc cctcacgaaa aagcaccaaa aaaatatcat tatcgaacaa
     2581 atgatgatat actttttaca tgctattcaa attgaggatg aattaaaaat atatcctaaa
     2641 atcattactg aaaatagacg aagcagcacc attgatattg aaatttttgt ggatgatcaa
     2701 gtgattgcta aagctattat tacaacgaaa ataaattaga ggagtaacaa aacaatgata
     2761 acattaaaat cagcccgtga aatagaagcg atggatagag caggagactt cttagcaggt
     2821 atccatattg gcttaaggga tattattaag cctggtgtgg atatgtggga agtggaagca
     2881 tatgttcgtc gccgttgtaa agaagacaac gttcttcctt tgcaaatagg ggttgatggt
     2941 cacatgatgg actatcctta tgcgacctgt tgtggtctaa atgacgaagt agcacatgcc
     3001 tttccccgtc attacattct gaaagaaggg gatttgctta aagttgacat ggtactcagt
     3061 gagcctttgg ataaatcaat tgttgatgta gcagctttag attttgataa cgtgcctgag
     3121 atgaagaaat ggacaggatc ttataccggt ggtttagctg actcttgttg ggcttatgct
     3181 gttggaacac cgtctgacga aataaagcaa ttaatggatg tcaccaaaga agccatgtac
     3241 cgtggtattg aaaaagcagt aattggcaat cgtattggtg atattggcgc agctgttcaa
     3301 gaatacgctg aaagcttcgg ttatggtgtc gtgcgtgatc ttgttggtca cggtgttggt
     3361 cctacaatgc atgaagagcc aatggtacca aactatggaa cagcaggtcg tggacttcgt
     3421 ctaaaagaag gcatggtctt gactgttgag ccaatgatta atacaggaac ttgggaaatt
     3481 gataccgaca tcaagacagg ttgggctcat aaaaccctag atggtggttt atcttgccaa
     3541 tacgaacacc aatttgtgat tactaaagat ggtcccgtta ttttaacaag tcaaggagaa
     3601 gaaagaactt actaaaatgg cagaaaaaaa atggtttgat aaggtactgt ccaagtggca
     3661 gtatgaaccc attcaagtct ttatgaggca tttacaaagt gcagaaatgg atttatctgc
     3721 cattgcggtt gcgtattacc ttatcttaac ggcctttcca ttaattgtta ttgcagccaa
     3781 tatttttcct tacctcaata ttgatattgc tgatttgtta cgtttaatga agcaaaattt
     3841 gcctaaagat atttttagac cggcttctgc aattgtagaa aatattttct ctaaaccgtc
     3901 tggaagtgtt ttgggagtag cgactttgac aggattatgg acaatgtctc gaagtttgac
     3961 atcgctgcaa aaagctatta ataaagccta tggcgcttct cagcatcgag atttctttat
     4021 cggacacttg gttggtttgt tgaccagttt aattatttta tttttattag cctttgcctt
     4081 aatcttttca acattttcta aagctgctat tcaagtgctg gataagcatt accatttgag
     4141 tgataatatc acaacgattt tcttactcct cattcaacct attacagttt tgattatttt
     4201 tgttggcttg atgctactat attttttatt acccaatgtt aagattaaaa aaattcgcta
     4261 cattttacca ggaaccctat ttacatcttt tgtgatgaca tttttgagta atctggttgg
     4321 caattacgtt gtgtataatg ttgagcggat ggtagatatt aaaatgtttg gttcagttat
     4381 gatttttatc atcatgctat ggtttatttt cttagcaaga atcctgattt taggagctat
     4441 atttaatgcg acctatcaag aaatgtcctt ggggaaatta gaaggtcgta gtggtgatat
     4501 gatcgctatc cttaaaaaaa cacttgggaa tgattcaggt ctatctcctt cacagtctat
     4561 cgaagatagt cacactgatt gatacaaaaa agataataag tcgtggagtg agaaactatt
     4621 ttcttgcttc attttttatg aggataaata gaaaatcatt gatcttagca aatagaaagg
     4681 cagataaaac ggatgaaatt gcgaacaaat gcaggaccat tacagggaac gattcaagtt
     4741 ccaggagaca agtccattag tcaccgggca gtcatattag gagctgttgc taaaggggaa
     4801 acacgagtaa aaggactctt aaaaggagaa gacgtgcttt ccacgattca agcctttagg
     4861 aatttggggg tccgtatcga agaaaaagat gaccagcttg tgatagaagg tcaaggtttt
     4921 caaggattga ccgctccttg tcagacactt aatatgggaa attctggaac ctctatgcgc
     4981 ctcattgctg gcttattggc aggtcaacct tttagtgtca aaatgattgg cgatgaaagc
     5041 ttatccaaac gtcctatgga tcggattgtt tatccgttaa agcaaatggg ggttgagata
     5101 tctggagaaa cagataggca atttcctcca ctacaattgc aaggtaatca taatttacaa
     5161 ccaatcacat ataccttgcc tatctcttct gctcaggtta agtcagctat tttattagca
     5221 gctttgcaag caaaaggaac aacacaggtt gttgaaaaag agattactcg aaaccataca
     5281 gaagaaatga ttcaacaatt tggtggacga ttgatagttg atggtaagcg cattactttg
     5341 gtgggacccc agcagcttac tgctcaagag ataactgtcc ccggagatat ttctagtgca
     5401 gctttttggc ttgtcgccgg cttaattatt cctggttcgg agctcctttt aaagaatgtt
     5461 ggagtcaatc cgacacgaac agggattctt gaagtggttg aaaaaatggg agcacagatt
     5521 gtttatgaag atatgaataa gaaagagcaa gtaactagta ttcgagtggt atattctcgc
     5581 ttgaaaggaa ctatcatatc aggaggtctt atccctcgat tgattgatga acttcccatc
     5641 attgcacttc ttgcaaccca agcacaagga acaacctgta ttaaagatgc tcaagaatta
     5701 cgagtcaaag aaacagatcg tattcaagta gtaactgata ctcttaacag tatgggagct
     5761 aatatcaaag cgacagcaga tggcatgatt atcaaaggcc ctacggtact atatggggct
     5821 aacacaagta cctatggaga tcatcgtatt ggtatgatga cagcaattgc agccttgctt
     5881 gttaaacaag gacaggttca tttggataaa gaagaagcca tcatgacgtc ttatccgacg
     5941 tttttcaaag atttagaaag gctgtgtcat gactaaagtg ttattgggat ttatgggagt
     6001 ggggaaaaca acggtttcga aacacctaag tatgcactgt aaagatatgg atgctattat
     6061 tgaagctaaa atcggtatgt ctattgcagc tttttttgaa cagcatggag aaatagcatt
     6121 tcgaacaatt gaaagtcaag tgttaaaaga tctattgttt acaaatgata atagtgtcat
     6181 cgtaacaggt ggcggtgtag tggttttaca agaaaatcga caattactga gaaaaaatca
     6241 ccagcataat attttattgg tagcttcttt tgaaactttg taccagcgtt taaaacatga
     6301 taaaaagtct caaagacctc tatttcttaa gtactctaag gaagcatttt atgaatttta
     6361 tcaacaacgt atggtatttt atgagggact ttcagattta gtaatcagag ttgaccatcg
     6421 tacgcctgaa gaagttgcca acatcattga gggttattag caggtcattg tcgtaatctg
     6481 atatttatgg ttttaaacag gtatctgtct aaagtacttc ttatggcaag ttggctttat
     6541 taaatattag gaaataatct tgattattct ataatgatta aaattttgag aggacaatct
     6601 ggctaatata ggttataatg gtagtattcg ttaaaagtgc aattgtaata ctaagagatg
     6661 ataagatatg acaaaatacc ccatgggtgg ccttagtcac catgaagaat tacgttattt
     6721 ttatctccta aggaatttga gttaccttag tgagaatgaa aaaaaagagt ttgctttttt
     6781 aaaatctaaa ttggaaattg gtcgagctta tgctccttct aagcagcatt ataggaagtc
     6841 aaaacggcaa gaaccttact ttgaagatga ctactataac gactactcac caaatgattt
     6901 actagaagat gatgacgtca atcatgactc ttcttttgtt ccatatggtt taccgatata
     6961 tcctaaagaa gacagatatt taaacaaaaa aacaaaactt acagctcgta gacctataga
     7021 tactccgcag cctattgatg aagacgatgc ttttttaaca gagtctgttg ctcgttgtgc
     7081 tcttcctaga tctcaaaaaa gaaagcataa gaaaaaagga tgtatgaaat ggttttttaa
     7141 catttttggc ttattgctaa tgactgtgct tatggggtta gggttgatgt ttgcaaaagg
     7201 ggtttttgat atttctacta ataaagctaa ttacaaacca gctgtttccc aagcttttga
     7261 tggtcaagaa actcaagatg gtacgaacat tctggttttg ggttctgacc aacgagttac
     7321 tcaaggttct acagatgctc gcacagatac cattatggtt gtcaatgtcg gcaaccatgc
     7381 taaaaaaata aaaatggtct cttttatgcg tgacactttg attaacattc caggatacag
     7441 ttataatgat aattcgtatg atcttaaatt aaatagtgca tttaatcttg gagagcaaga
     7501 agatcaccac ggagcagagt acgttcgacg ggccttaaaa cataattttg atatcgacat
     7561 taagtattat gtgatggtag attttgaaac atttgcagaa gctattgaca ccttgtttcc
     7621 aaatggtgtt aagattgatg ccaaatttgc gacagtaggt ggtgtagctg ttgactccgt
     7681 agaagttcca gatgacctgc gaatgaaaaa tggggttgtt cccaaccaaa ccatcgaagt
     7741 tggagaacaa cgcatggatg gtagaacctt gctcaactat gctcgtttcc gtaaagatga
     7801 cgaaggagat ttcggacgaa ccgtccgtca gcaacaggtt atgtcagctg ttatgtccca
     7861 aatcaaagac ccaactaaac tctttacagg gtctgcggct attggtaaaa tctatgcgct
     7921 gacttcgacc aatgtttctt ttccatttgt tgttaaaaat ggtgtttctg ttttaggaag
     7981 tgggaaaaat ggagttgagc acgtgaccat cccagaaaat ggggattggg ttgacgaata
     8041 cgatatgtat ggaggacaag ctctttacat agattttgat aagtatcaga aaacgttagc
     8101 caaattaggt ctgcgctaag atatggtata atggagagta actaaagttg ctcttctttt
     8161 ttgagcaatg gttagcccta gaaaaggaaa aagaataaga atgttgaaaa aaaatgatat
     8221 tatccaagtt gctatttcag atttaagcca tgagggagca ggtgtcgcta aacacggtgg
     8281 ttttgttttc tttgttgata atgccttgcc agaagaagtg attgacatgc gtgtcttaaa
     8341 ggtcaataaa aacagcggtt ttggcaaagt agaagtctat cattacctgt cgccagctag
     8401 aaatgcagat gtcaatttaa cttatttgcg aacaggcatt gcagaccttg gacatctaac
     8461 ttatgaagac caactgactt tcaaaaagaa acaagttcag gacagcctct ataaaattgc
     8521 tggaatcagt gacgtgacag tcgaaagcac gattgggatg acagaacctt tagcttaccg
     8581 aaataaggct caagttcctg ttagacgtgt taacggtcag ctagaaacag gctttttccg
     8641 caagcattct catgacttga ttcctatttc ggattattat attcaggaca aagagattga
     8701 tcgtctcatt aattttaccc gtgatttatt acgccgcttt gatattaaac cgtatgacga
     8761 aacagaacaa acaggtctct tgagaaatat tgttgttcgc cgaggccatt actctggaga
     8821 aatgatgctg gtcttagtga ctacacgccc aaaagttttc cgtgttgacc aagtcattga
     8881 aaaaatcgtt gaagcttttc cagcagtggt gtctattatt caaaatatta atgataagaa
     8941 caccaatgca atctttggta aagatttcaa aacgctttac ggcaaggata ccattactga
     9001 tagcatgctt ggaaacaact atgccatttc tgctcagtct ttctaccaag tgaatacgat
     9061 tatggcagaa aaactctatc aaacagctat agccttttca gacctttcta aagatgacat
     9121 tgtgattgac gcctactcag gtatcggaac cattggccta tcatttgcta aaacagttaa
     9181 agctgtttat ggtgttgaag tgattgaagc agctgttaga gatgctcaac agaacgccgc
     9241 tcttaatggc atcaccaatg cctactttat cgcagatacc gcagaacatg ctatggcaac
     9301 atgggcgaaa gatggcatta aaccaagtgt tattctggta gacccgccac gaaaaggctt
     9361 aacagaaagt ttcatccaag ccagcgtggc aatgggacca caaaaaatca cctacgtttc
     9421 ctgcaaccca gccaccatgg cgcgtgacat caagcattat caagagttgg gttacaaact
     9481 gaccaaagta cagcccgtag atttgtttcc ccagacgcat cacgtggagt gtgtaagttt
     9541 acttgagaaa cggagttaaa gagtccgcct cactctgtta tttcaaggtg agacgctcaa
     9601 aaggtccact ggaccttttg actcccgcag acccatcacg tcgagtgcgt agtgttgcta
     9661 atcaaggagt aaggtagctg cctccacatg acaaaaataa ggagaaacga atgcctatta
     9721 ccaaacacga gattcccctt ttagaatgtg atagtaatcc atcagccgtc attatctagc
     9781 tatttagctt ccagctaagt gtgtctatgc tttcttagaa gacgagattg aagcttttgc
     9841 tttagaccat caaggtaaaa aagtagcaga attcatttca gccacaaagg tttaccctgt
     9901 ttatcagata tcttataaag gtcaagacat ttgcctagct caagctcccg ttggctcagc
     9961 tcctgcggcg caattcatgg actggctgat tggttatggg gttaaagaaa tgatcagtac
    10021 aggaacctgt ggcgttttgg ttcctatcgc agaaaaccgt tttttagtgc cagttaaggc
    10081 tttgcgagat gaaggaacaa gttatcatta cgtggcacca tcttgttata ttgatattga
    10141 tcctaagatg cttagaccga tagagaaaac gctttcagca caggggttag cttatcaaga
    10201 ggtcatcacc tggtcaacag atggttttta ccgagaaacc aaagaaaaag tggcttatcg
    10261 ccaggaagaa gggtgttcgg ttgttgagat ggaatgttct gccttagcag cagtagctca
    10321 attaagaggc atcctttggg gacaactctt atttacagca gatactctag cagatgtaga
    10381 ggtttacgat caacgcaacc ggggcgctga ttcctttagt tttgcgcttc atttatgctt
    10441 ggaagtgttc aatacgctag aaaaggatgg aaaggctact gatttttgaa acatgagctc
    10501 tgccttctgt tcctatttgt agtataatga ggcttgtatt gcttttagac taagcttaga
    10561 aaagagaaaa acaatgtcag atttaacaca agagatgacc ttaaatgtta ttagtatttt
    10621 tgataattac caaattgaac ttgctgaaaa aggacacctt attttaagta ctgaagtgac
    10681 tgagacggct ttgaattatt atggggatgc ccatggtggt tatcttttca ccctatgtga
    10741 tcaagtaggt ggtttggttg ctagaactac tggcgtagaa tctgtcacct tacaggccaa
    10801 tgctaactat ttaaaagcag ggcacaaggg agacaagctg atggttgaag gtcgattggt
    10861 tcatggaggt cgaaccacgc aggtggttga tgtgacgatt cataaccaaa caggagctct
    10921 tctaacaaaa acaagcttga ccatgtttgt gactggccga cgtcataacg ggtgattaat
    10981 gaatcaataa aggaaaaaag tcccaactct ctgagaattg ggactttttt cctttattga
    11041 gagataccat aaatcacccg ttagacgggt ggttatggtt tatttacggt aaagtttgtg
    11101 atgttggtaa acagggtcaa aggtaatatt taccccaagt tttcggagta catttttatc
    11161 ttcttcggtt aagataacgg tagaatgggc ttcgctacct ttcaaattgc cgagttcttt
    11221 catggctaga ttagcttctt cactagtcat agcagtaatg gccaacgcaa tcagaatttc
    11281 attggaatga agccgcggat tgtgactgcc taagtggttc actttgagcc cttgaatagg
    11341 tttcacgtat tcgggttcaa tcaaatgagt ggttttatca atctttgcca aggttttaat
    11401 cgcattgatg ataacagctg cagttggtcc aaagagttca gatgttttac ctgtcacaat
    11461 ctgcccattt ggtagttgga gagcaagggc aggttggcct gtttgctctg ctttttgatg
    11521 agcagctaca gtaacgtgac gatcgtcagg ggtgacaccg atatcattca tcaagagctc
    11581 aatttttttg acagcagatt ctgtgacccg ctctgcctta aaatccacga gggtttggta
    11641 ataacgacgg atgatttctt gtttagaagc ttcgatagca gcttcttcat tgacgataga
    11701 aaaaccaacc atgttaacac ccatatcggt tggtgaggca taaggagact tgctcaaaat
    11761 acgctcgaag gtccgattta agacaggaaa gacctcgata tcacggttgt aattaacggc
    11821 cgttttgcca taagtttcca aatggaaggg gtcaatcata ttcacatcat ctaggtctgc
    11881 cgtggctgcc tcataagcca gattgacagg gtgatgaaga ggaagattcc aaactgggaa
    11941 ggtttcaaat ttagcatagc cagacttaac cccattgatt tggtcgtgat acatttggga
    12001 catgcaagtg gctaattttc cagacccagg gcctggtgct gtaacaacga taaggttacg
    12061 actcgttttg atgtaatcat ttttccccat cccctcagat gagataatat gattaatatc
    12121 tgtcgggtag cccttgatag gataatgcag gtaagaagca atgccatttt tttcgagttg
    12181 cttgcgaaag gcatctgcag cgggttggtt attgtattgg gtaataacga cagaacctac
    12241 gtaaatatct aacgtgttaa atttgtcaat taagcgaaag acttcttggt cataagaaat
    12301 gcctaaatca ccacgcgctt tagaatgttc aatattatta gcgttaatgg caattacaat
    12361 ttcgacttgt tctttgagct ctttgagtaa tttaatctta ttgtcaggtt catagccagg
    12421 aagcacacgg gcagcatggt aatcttctaa cattttacca ccaaattcca tgtaaagttt
    12481 gccatcaaat tggctgatac gttctaaaat atgatctctt tgcaagttta aatacttgtt
    12541 agaatcaaaa gcaatagtct tcattttttc acctctgtaa agcattataa caaagaaata
    12601 ccaagaaaga aaaggttttt tgcagggata cctcattttg tgacttttta aaaaaactta
    12661 ggcttggttc ttagaagaaa aatgtatccg ctatgttcaa aagaacttga caattaaaat
    12721 gagctttact atactagtaa caattaaata ctactttaaa catgttccgt gaagcatgaa
    12781 agtcttaaca gtaaagagag tttttttgtc atctcttatt atttagtgaa ctttgatgga
    12841 cttgcggact tgtttccaag tctttttttg acctttggac aaagggtatt caattaatta
    12901 aggtagtcag tgagctacta ttaagaagga gacaattatg tcaacattat cattggacac
    12961 cacaaataag cgagctttag tggcagctat tgcagcttca ggaacggatg acttgaatgt
    13021 catgtttctt gccttttcca tgtcctccat tatgacagac ttgggtctta gtggcactca
    13081 gggtggctgg attgcgacca ttaccaattt aggaatgttg gtgggaggcc tcttattcgg
    13141 gttgctggct gataggcacc acaagtttaa ggtctttaaa tggacgattt tactgttttc
    13201 agtagcaaca ggattaattt attttaccca gtctttgccc tacttgtact tgatgcgatt
    13261 tattgctggc ataggagttg gaggtgagta cggtatagcc attgctataa tggcagggat
    13321 tgtaccacca gaaaaaatgg gtcggatgtc ttctttaaat ggtattgctg gccagcttgg
    13381 ctccattagt tcggctcttt tagcagggtg gctggctcca agtctaggtt ggcgtggcct
    13441 ttttctcttt ggattacttc cgattctctt ggtgatttgg atgactttag ccatagatga
    13501 ccaaaaaatt tgggatcatt atgggcaaga ggaggaagaa tgcagtcaac caattaaaat
    13561 caatgaacta ttcaaaacaa aatccttaac agctcaaacc cttgctttaa tggtcatgac
    13621 gactgtgcag attgcgggct attttggaat gatgaattgg ttgccaacca ttattcaaac
    13681 aagcttaaac ctttcagtaa aaagttcgtc cttgtggatg gtggctacta ttgtcggcat
    13741 gtgtttgggg atgctgtgtt ttggtcagct tttagattgc tttggtccac gtctgattta
    13801 ttccctcttt ttattagcat catctatttg tgtttacctt tttcaatttg ccaattccat
    13861 ggcgagtatg gttattggag gtgctattgt cggttttttt gtcaatggga tgtttgctgg
    13921 ctacggggct atgattacca gactttatcc ccatcacatt cgatccacgg ctaataatgt
    13981 tattttgaat gttggccgtg ctctaggagg attttcgtcc gttgctattg gtagtatttt
    14041 agatgcctca ggtatttcaa tggtcatgat tttcctagct tctctgtatg tgattagttt
    14101 tggagccatg tggactattg gtcagttaaa agcagaacgc taccaacaat tgcgttagca
    14161 aagagatggg tgttagtatt tgagttactt tcactcacaa agctatttta ttgattgaga
    14221 taatcaaaaa ggtaagtact tagggattgc ttaccttttt gattcttttc ttttgtttta
    14281 ttccgctact ttggccataa aatgtttact gttcacccta actctagtat gacaggcttt
    14341 tccaatgagt tgttctccca caaaagcttc caagcggaaa tcgtattttc ggccttcttc
    14401 tttaagggct gtaatgatga ttgtaacagg atctccaatg gctgaagctg ccaaatgttg
    14461 gatagctatc tcgctgccaa ccgttgtaaa gtcactgtcg agctgttctt gggcaaataa
    14521 gtaagaagca ttttccataa aagcaacaag agcaggagta gctaggacat tcagcgttcc
    14581 tgagcccatg gctttagccg aatgattggg atgggtttca taagtgtgag aataaatagt
    14641 cataagttaa tctcctcgtt tctataagcc tattataata tattaagcgc ttacagtcat
    14701 gaaaccactg tttttttatg ttatacttaa taatgaattt tctttgaaag ggttatgatg
    14761 cgtttagata aatttttagt ggcaactggt gttggaacta ggagccaagt caaacttctc
    14821 ttaaaaaaga aggctatctt cgtcaatcaa aaggttgaaa cctctgccaa agcacacatt
    14881 gatgaataca aggatttggt gacgtatcaa ggcacgccct tggtctatga atcctttgtt
    14941 tattacctgt taaacaagcc gtcagggtat gtgtcggcaa cccaggacag acagcaggct
    15001 actgttatgg agttgttaga tgacacggct aggcaaaaaa cagtttttcc tgtgggtagg
    15061 ttagataagg atacccgtgg acttttgctc ttgaccaata atggacaact agcccatgac
    15121 cttttatccc ctaaaaaaca tgttacgaaa gagtacttgg ctaaagtagc aggcatcatg
    15181 actgaagctg ataaagatta ttttgctaga ggtatttcct taaaggatca ccagtgccta
    15241 cctgctcatt tagaagtgct tgctagtgat ttacagcagc aaacaagttt ggttaaaatt
    15301 accatacaag aaggaaaatt ccatcaggtg aaacgcatgg tagcagcctg tggaaaggaa
    15361 gtgcttgact tgcagcgtct cagcatggga ccattaaaac tagaccctag ccttgcagaa
    15421 ggggagttta ggcgcctgac gcctgaggaa ttgcagagcc tggcacctta ttgtcagagc
    15481 tcactataaa aagaaacctc cccggtcagt acacttgctc aatagttgcg aaatgagcat
    15541 cattgacaag ctctcttttt aatgttatat aaaaggcttc catgagtgca ttatcgtatg
    15601 gattaccttt tcgactcata ctggacttac attttttccg tctaagtagg tcctgaaatc
    15661 gtgctccggt atattgagaa ccctgatctg tatggacgat taccccttct ttaggctttt
    15721 ctctattata agcttgattg aaagcttctg ttaccaactt gtcttgcata cgtcggccca
    15781 ttggccaacc aacaatcttt ctactataaa cgtctatcaa gacagacaca taaagtattc
    15841 cttcttgtgt aggaatgtag gttaagtctc ctagctataa tttattctta ccagtcgctt
    15901 ggaaacattg attaacaaga ttgggacgag ttaatgaaga acgtcgacgg ttatagtatt
    15961 tatattgata tcgacttcct ttagcataga gacctagctg atgaaggagt ctaccaacac
    16021 gtttatgatt gacatggatg ccacgtctac aaagttcttg ggtaattcgt acacttccgt
    16081 aacgctcttt atgttcatag aaaatgtctt taatcatttc cttcaaagct tcattctcaa
    16141 catgctttga agatagacga cgttttaaat aagcatagaa acctgaacgg gagacttgaa
    16201 gagtttgaca ggcgtgatga ctattcaagc taacactatt ttccttcaga aactgaaatt
    16261 ttatttccgg tttggcttca agaagacctg gaactttttt agaagagcta attcctcctg
    16321 tagcagtttg ttctcttttt caagtttttt t
//
LOCUS       DAUELZ010000036        16001 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.036, whole genome shotgun sequence.
ACCESSION   DAUELZ010000036 DAUELZ010000000
VERSION     DAUELZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16001
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.036"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            11..802
                     /locus_tag="VHI09_000878"
     CDS             11..802
                     /locus_tag="VHI09_000878"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEQ6120764.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGVDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            876..1310
                     /locus_tag="VHI09_000879"
     CDS             876..1310
                     /locus_tag="VHI09_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ6120765.1"
                     /translation="MEYDKINHYLVDIFNRILVIEEMSLKTSQFNDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            1311..2285
                     /locus_tag="VHI09_000880"
     CDS             1311..2285
                     /locus_tag="VHI09_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEQ6120766.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLANIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVKI"
     gene            2346..2570
                     /locus_tag="VHI09_000881"
     CDS             2346..2570
                     /locus_tag="VHI09_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ6120767.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            2771..3742
                     /gene="fabK"
                     /locus_tag="VHI09_000882"
     CDS             2771..3742
                     /gene="fabK"
                     /locus_tag="VHI09_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEQ6120768.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            3767..4705
                     /gene="fabD"
                     /locus_tag="VHI09_000883"
     CDS             3767..4705
                     /gene="fabD"
                     /locus_tag="VHI09_000883"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEQ6120769.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            4695..5429
                     /gene="fabG"
                     /locus_tag="VHI09_000884"
     CDS             4695..5429
                     /gene="fabG"
                     /locus_tag="VHI09_000884"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEQ6120770.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            5444..6676
                     /gene="fabF"
                     /locus_tag="VHI09_000885"
     CDS             5444..6676
                     /gene="fabF"
                     /locus_tag="VHI09_000885"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEQ6120771.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMIDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQEREATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            6678..7178
                     /locus_tag="VHI09_000886"
     CDS             6678..7178
                     /locus_tag="VHI09_000886"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEQ6120772.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNPTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            7175..7597
                     /gene="fabZ"
                     /locus_tag="VHI09_000887"
     CDS             7175..7597
                     /gene="fabZ"
                     /locus_tag="VHI09_000887"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEQ6120773.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            7629..8993
                     /locus_tag="VHI09_000888"
     CDS             7629..8993
                     /locus_tag="VHI09_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEQ6120774.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEIIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAY
                     GNIIHLGERDCSLQRNNQKVLEESPSIAIGNTLRHEMGQAAVRAAEAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAINQEDITITGH
                     AIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            9002..9868
                     /locus_tag="VHI09_000889"
     CDS             9002..9868
                     /locus_tag="VHI09_000889"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEQ6120775.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKGQQIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            9865..10635
                     /locus_tag="VHI09_000890"
     CDS             9865..10635
                     /locus_tag="VHI09_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEQ6120776.1"
                     /translation="MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYQMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(10868..12145)
                     /gene="serS"
                     /locus_tag="VHI09_000891"
     CDS             complement(10868..12145)
                     /gene="serS"
                     /locus_tag="VHI09_000891"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEQ6120777.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRTLLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGETVISPK"
     gene            complement(12537..12896)
                     /locus_tag="VHI09_000892"
     CDS             complement(12537..12896)
                     /locus_tag="VHI09_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEQ6120778.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGLGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            complement(13010..13921)
                     /locus_tag="VHI09_000893"
     CDS             complement(13010..13921)
                     /locus_tag="VHI09_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ6120779.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            complement(13938..14747)
                     /locus_tag="VHI09_000894"
     CDS             complement(13938..14747)
                     /locus_tag="VHI09_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEQ6120780.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAAAEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            complement(14836..15828)
                     /gene="manX"
                     /locus_tag="VHI09_000895"
     CDS             complement(14836..15828)
                     /gene="manX"
                     /locus_tag="VHI09_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEQ6120781.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
BASE COUNT         4858 a         2898 c         3590 g         4655 t
ORIGIN      
        1 gaggattgtt atgcagttta aacacatcat ctttgatgtt gtagatgatc ttgcgacatt
       61 gactttaaac cgtccagagg tttcaaacgg ttttaatatt ccaatttgtc aagagattct
      121 ggtagcatta gcagaagtta aacgcgatac atctgtgcgt tttttactaa ttaaagccgt
      181 tggaaaagta ttttcagttg gtggtgacct agttgaaatg caagaagctg ttgcaaaaga
      241 taatgtgcag tcgctagtca agattgctga actggtacaa gaaatttctt ttgcaatcaa
      301 gcatttgcca aaaccagtca tcctttgcgc cgatggagct gtagctggag cggcttttaa
      361 tatagcttta gctgttgact tttgtatcgc tagtactcaa acaaaattta tccaagcttt
      421 tgtaaatgtt ggactagcac cggatgccgg tggtttattt ctattgactc gtgcagtagg
      481 acttaatcgt gcaactcatc ttgtgatgac aggtgaagga attactgctg ataaaggtgt
      541 cgattatggg tttgtctacc gtacggcaga atctgataaa ctagacaaag tttgtcttca
      601 acttttaaag cgattgagac gtggctcttc aaattcttat gctggaatga aatcattggt
      661 ctggcaaagc tttttcacag gatgggaaga ttatgctaag gcagagcttg ctattcaaga
      721 ggagctagct ttcaaggaag attttaaaga aggtgtcata gcttttggag aaaggcgccg
      781 accaaatttt caaggtaagt agactcatgc ttgcaaagtg cttttaactc tgatataatt
      841 tgactgtcaa agttttattg ttaggaggta tttgcttgga atatgacaaa atcaatcact
      901 acttggttga tatcttcaac cgtattttgg taattgaaga gatgagttta aaaaccagtc
      961 aatttaatga tgtctcttta aaagagatgc acactattga aattatcggc aaatatgatc
     1021 aggtgactcc aagtgatatc gccagagaat taatggtaac gttagggaca gtaaccacta
     1081 gcttaaataa attagaagca aaaggttata tcgcaagaac gcgatcacgc tcagataggc
     1141 gagttgttta cctgtcatta acaaaaaggg gtcgactttt ggaccgcctc catgcaaagt
     1201 tccataaaaa tatggttgga catgtcattg ctgatatgag tgatgaagag atgcaggcat
     1261 tggtgcgtgg actagggaat ctacatcaat ttttggagga tttggtttaa atgatttttt
     1321 ctaaaatcag tcaggtagcc cactatgttc cacagcagtt agtgactaat aatgatttag
     1381 ctaacataat ggacactagt catgagtgga tcttttctcg aacaggaata gcagaacgcc
     1441 atatttctag agatgaaatg actagtgacc tagctattca ggttgctgat cagcttttaa
     1501 ctcagtctgg gttaaaagct gatgctattg attttattat tgttgcaact atcagccctg
     1561 atgccactat gccatcaaca gctgcgaaag ttcaagcagc tattgcggct acaagtgctt
     1621 ttgcttttga tatgactgct gcttgtagtg gctttgtctt tgccttggct atggcagata
     1681 agcttattgc atcaggtgcc taccaaaacg gaatggtaat aggtgcagag actttatcta
     1741 agttagtaaa ctggcaggat cgagcgacag ccgttctttt tggagacgga gctggtggcg
     1801 tgctactgga agccagtaaa gataagcatg ttttagcgga aacattacac acagatggtg
     1861 cgagatgtca gagtttgatt tcaggagaaa ctagtctttc atctccttac tcaattggca
     1921 aaaaagccat agcgactatt cagatggatg gacgtgccat atttgatttt gcgattaggg
     1981 atgtgtcaaa aagcatcctc acactaatgg cgcaatcaga tattacaaaa gatgacatag
     2041 actattgttt attgcatcaa gctaatcgtc gtatacttga taaaatagca cgcaagattg
     2101 atgttcctcg ggaaaaattc cttgaaaaca tgatgcgtta tggtaatact agtgctgcta
     2161 gtatcccaat cctcttgtca gaggctgttc aaaagggaca gattaggtta gatggtaccc
     2221 aaaaaatctt actttccggt tttggtggag gtttgacatg gggaagtcta attgttaaaa
     2281 tctagttatt catgtgccta gcacatttat aaaaacaaat ttatattttt taaggagaaa
     2341 taaacatggc agtatttgaa aaagtacaag aaattattgt tgaagaactt ggtaaggaga
     2401 cagaagaagt aacactagaa actacatttg atgatttaga tgcagattca cttgatgttt
     2461 tccaagttat ttcagaaatt gaagatgctt ttgatattca aattgaaaca gaagaaggat
     2521 taaatactgt tggtgacctt gttgcttacg ttgaggaaaa gtcaaaataa ggataaaagt
     2581 gacaagtaac atcagaagtc tatgcaaagc ttactgctgt tatgttcatg ttcatcacta
     2641 aaaaaacaat aagtattaat caaaccatta gggtgggatt agactctttt taggatctag
     2701 ctcgctcttt cttcttacta taatagtttg attatcaaat tattctcctt attttttata
     2761 gaaagttatt atgaaaacac gtattacaga attacttaat attgattatc ccatttttca
     2821 aggaggtatg gcctgggttg ctgatggtga tttagcagga gcagtgtcca atgctggtgg
     2881 gctaggtatc atcggtggtg gcaatgctcc caaagaagtc gttaaagcca acattgaccg
     2941 tgttaaagct attactgata gaccttttgg ggttaatatc atgcttttat ctccatttgc
     3001 tgatgatatc gttgatttgg tcattgaaga aggtgttaaa gtagtaacaa caggcgcagg
     3061 aaatccagga aagtatatgg aaagactgca ccaggcgggt ataatcgttg ttcctgttgt
     3121 cccaagcgtt gcgctagcca aacgtatgga aaagcttggg gtagatgctg ttattgctga
     3181 gggtatggaa gctggaggac atattggcaa gttaacgact atgtctttag taagacaagt
     3241 tgttgaagcg gtttcgattc ctgtcattgc ggcaggtggt atagctgatg gtcatggtgc
     3301 agcagcagca tttatgttag gagcagaggc tgttcaaatt ggaactcgct ttgttgttgc
     3361 taaagaatcc aatgctcacc aaaattttaa agataaaatc ttagcagcaa aagatattga
     3421 tacggtgatt tctgcgcagg ttgtgggcca ccctgtccgt tctattaaaa ataaattgac
     3481 ctcagcttac gctaaagcag aaaaagcatt tttaattggt caaaaaacag ctactgatat
     3541 tgaagaaatg ggagcaggat cgcttcgaca tgctgttatt gaaggcgatg tagtcaatgg
     3601 atctgttatg gctggccaaa ttgcagggct tgtgagaaaa gaagaaagct gtgaaacgat
     3661 tttaaaagat atttattatg gtgcagctcg tgttattcaa aatgaagcta agcgctggca
     3721 atctgtttca atagaaaagt agataaacac atgaaggata atgataatga caaagacagc
     3781 ctttttattt gccggtcaag gtgcccaaaa attagggatg gcaagggatt tttatgataa
     3841 ctttgctatt gtaagaaaaa cctttgatca agctagtcaa gtattgggat acgatttgcg
     3901 ccgtttgatt gatagtgacg agttaaaact taatcaaact agctatacgc aaccagctat
     3961 tttgacatca tcaattgcta tttaccgtgt tttgggctta catcacgtta aaccggatat
     4021 ggtagctggt ctttccttag gagaatactc agctttggta gcatcagggg cactctcttt
     4081 tgaagatacc ctatccttag tagctaagag agggcgcttg atggaggaag cagcaccaca
     4141 aggatctgga aagatggttg ccgttatgaa tacagacgtg caagtcatcg aagaagtctg
     4201 tcaaatagct gctaaacatg gagtggttgc tccagcaaac tataatactc ctagtcaaat
     4261 tgtaattggt ggtcagacag atgctgtgaa cgtggcagtt gaacttttaa aggaaagggg
     4321 agttaagcgt ttaatccctt taaacgtgtc aggtcctttc cacactgctt tgttagaacc
     4381 agctagccgc ttgttggcta aagagttgga aagatacaac ttcagtgact tcaagattcc
     4441 tttggttggt aataccgaag ctaatattat ggaaaaagac cgtatcccag aactattagc
     4501 ccgtcaagtc atggagcctg ttcgttttta tgacagtgtt gcgactttag tagaaagtgg
     4561 cataacacaa ttcattgagg taggaccagg taaggttttg acaggttttg tgaagaaaat
     4621 tgataaaaat ttactatgta ctagtgttga aaacatggta agcttacgtt tatttttaga
     4681 tagagaggac attcatggaa attaaaggaa aaaatatatt tatcacaggc tcaacgagag
     4741 gtatcggttt agcaatggct catcaattcg ccagtttagg agctaatatt gtcttaaatg
     4801 gtcgctctgc tatctctgag gagcttgttg ctagttttac tgactacggt gttacagttg
     4861 ttaccatctc tggggatgtt tcagaagcat cagaagctaa acgtatggtc aatgaagcta
     4921 tcgaaagttt aggttcgatt gatgttttag ttaacaatgc tggcattact aatgataaat
     4981 tgatgcttaa aatgacggaa gaggattttg agcgcgtctt gaaaatcaat ttgaccggtg
     5041 cttttaacat gactcaatca gtgttaaaac caatgatcaa ggcccgccaa ggagcaatta
     5101 ttaatgtctc tagtgtggtt ggtttaacag gcaatattgg gcaagcaaac tatgccgctt
     5161 ctaaggcagg aatgattggt tttaccaagt ctgttgcccg tgaagttgct gctcgtaaca
     5221 tttgcgtgaa tgctattgct ccaggattta tcgagtccga catgacaggt gtccttcctg
     5281 aaaaaatgca agagcaaatc ttaagtcaaa tcccaatgaa acgtattgga aaagctcaag
     5341 aagttgctca cttagcaagc ttcttggtgg aacaagatta tattacggga caagttatcg
     5401 ctattgatgg cggtatgaca atgcaataag gagacgtagt gaaatgacat ttaaaagagt
     5461 agtggtaaca ggttatggtc taacatctcc aatcgggcat gatcctgaaa cgttttggaa
     5521 caatctgaaa gctggccaaa ttggtattgg ccctattact aaatttgaca cgactgatta
     5581 tgcggtcaag aatgctgctg aaattcagga ttttccattt gacaaatact ttgtgaaaaa
     5641 agatttgaac cgtttcgata gatattctct ttatgctctt tacgcagcga aagaggctat
     5701 taatcatgct gatttaaata ttgagatgat tgattccgat cgatttggcg ttattgtcgc
     5761 ttcaggtatt ggtggaattg cggagattga agagcaggtg attcgcttac atgaaaaagg
     5821 gccaaaacgt gtgaagccaa tgacgttgcc aaaagcgcta ccaaatatgg ctgcaggaaa
     5881 tgtcgccatg tctttaaaag ctcaaggagt atgtaaatca attaatactg cttgcgcttc
     5941 ttcaaatgat gcgattgggg atgcctttcg tgccattaaa tttgggactc aagatgtgat
     6001 gatagttggt gggtcagagg cggccataac caagtttgcc attgctggct tccaaagttt
     6061 aacagctctc tcaacaactg aagatccaag cagaagctct attccatttg ataaagaccg
     6121 caatggtttt atcatgggag aaggatcagg aatgcttgtt ttagaaagcc tagaacatgc
     6181 gcaagaacgc gaggcgacaa tcttagcaga aattgtgggt tatggaaata cctgcgatgc
     6241 ctaccatatg acatctccaa atcctgaggg attaggagct cgtaaagcca tccacttagc
     6301 cttacaagaa gctggtattg aagccagtgc tattaattat gtcaatgcgc acggaacttc
     6361 aaccccagct aatgaaaaag gagaaagtca agcaattgta gccgttcttg gtaaggatgt
     6421 tccggtgtct tcaacaaaat cgtttacggg tcaccttctt ggagcagcag gcgctattga
     6481 agctattgcc actatcgagg ctatgcgtca taactatgtt ccgatgacgg ctgggacaca
     6541 agctttatca gaggatatcg aagcaaatgt catctttgga gaaggaaaag aaacagctat
     6601 caactatgcc atttcaaata cttttggatt tggtggtcat aatgctgtat tagctttcaa
     6661 atgttgggag gagtaggttg aatattcaag aaatcaaaga tttgatggct cagtttgata
     6721 cctcaagcct acgtgaattt ttattcaaaa caaatgaagg tgagctaatc tttagtaaaa
     6781 acgaacaaca cctcaatgca tcgacatcaa atcaagaaca tgctgtgcct gtaccacagg
     6841 tacaacttgt tccgaatcca actgcttcgg aagcgtccag tccagcatca gttaaggatg
     6901 ttcctgtaga ggagcaacca caagcagaat cttttgtagc agaaggtgat attgttgaaa
     6961 gtcctttagt cggtgtagcc tatcttgcag ctagccctga caagccacca tttgtggcag
     7021 tcggagatac tgtaaaaaaa ggacaaaccc ttgtgattat cgaagccatg aaggtgatga
     7081 acgaagtgcc agcgccatgt gatggtgtga ttacagaaat tttggtatca aatgaagatg
     7141 tcattgaatt tggacaagga ttggtgcgga tcaaatgatg gatattagag aaattcaagc
     7201 agctttgcct catcgctacc caatgttatt ggttgatcgt gtgttagaag tgtcagacga
     7261 ccatatcgtt gccattaaaa atgtgactat taatgaacct ttctttaacg ggcattttcc
     7321 tcactatcct gtgatgccag gtgttctgat tatggaagct ttggctcaaa cagctggtgt
     7381 cttagaattg tcaaaagaag aaaataaagg caagttggtc ttttacgctg gaatggataa
     7441 agtgaagttc aaaaaacaag tcgtccctgg tgatcaactt gtgatgacag caacctttat
     7501 caagcgtcgc ggtaccattg ctgtcgttga agccagggca gaagttgatg gcaagctagc
     7561 agcaagtgga acattaacct ttgcctgtgg gcaatgagaa tgattgttct gcggaggaag
     7621 aaacccttat gtttaaaaaa atcttaattg ctaatcgtgg tgaaattgca gtgcgtatca
     7681 ttcgtgctgc acgagaatta gggatttcca cagttgcagt ttattcggaa gcggataagg
     7741 aagctctgca cacgatattg gcggatgagg ctatctgtat tggtcctgcc cgttccaagg
     7801 aatcctatct taatatgaat tccgtcttgt cagcagctat tgtgacagga gcccaagcga
     7861 ttcacccagg gtttggcttt ttaagtgaaa actcaaagtt tgcgaccatg tgtgaagaga
     7921 tgaatattaa atttatcggt ccatccgcat cggttatgga taaaatgggt gataaaatca
     7981 atgctcgttc tgaaataatt aaggcaggag tgcccgttat tccagggtca gatggtgaag
     8041 tttataacgc tcaagaagcc ttagctattg ccaataagat tggttatcct gtcatgctaa
     8101 aagcatcggc aggtggtgga ggtaaaggga ttcgtaaagt ggagacagaa gcagatttgg
     8161 aggcggcttt caatgctgcc agtcaagaag ctttgggagc tttcggaaat ggcgccatgt
     8221 atttggaaaa agtaatttac ccagctagac atattgaggt gcaaatttta ggagacgctt
     8281 acggtaacat cattcacttg ggagaacggg attgctcctt gcaacggaat aatcaaaaag
     8341 tcttggaaga aagtccatcc attgctattg gcaataccct cagacacgaa atggggcaag
     8401 cagcggttag agcagctgaa gctgtagcct atgaaaatgc gggaaccatt gagtttttgt
     8461 tggacgaaga cagcgagaaa ttctatttca tggaaatgaa tacccgtatc caagttgagc
     8521 atcctgtcac cgaatttgtg acaggagtgg atattgtcaa agagcaaatc aaaattgcag
     8581 caggtcagcc tttggcgatt aatcaagaag acatcactat cacgggacat gctattgaat
     8641 gtcgtatcaa tgctgaaaat actgctttta actttgcacc gagtccagga aagattacag
     8701 acttgtatat gccaagtggt ggtgtaggac tacgagttga ttcagcagtt tacaatggct
     8761 atgccattcc tccttattat gacagtatga ttgctaaaat catcgttcat ggaagcaatc
     8821 gatttgacgc tttgatgaaa atgcaacgtg ccttagttga attggaaatt gaaggcatta
     8881 tcaccaatac agacttccag ctcgatttga tttcagataa gcgagtgatt gcaggagatt
     8941 atgatacctc tttcttgatg gaaacgttcc tgccacatta caataaagat taggagaaaa
     9001 tatggcttta tttcgtaaaa aagataaata catccgtatt actcccaata attccctcaa
     9061 aggatctgtc agccataatg tcccagaagt tccagatgaa ctctttgcga aatgcccagc
     9121 ttgtaagcac atgatttata agaaagattt gggattggca aagatttgcc caacctgttc
     9181 ttacaatttt aggatttctg cccaagagcg gctgacctta acagttgatg aaggctcttt
     9241 ccaggaatta tttacaagta ttgaaaccaa ggacccgtta cgctttccag gttatcagga
     9301 aaaattacaa aaagccaagg aaacaactgg tctgcatgaa gctgttttga cagggaaagc
     9361 tatggttaaa ggacaacaaa ttgctcttgc tattatggat tctcacttta ttatggctag
     9421 tatgggaact gttgttgggg aaaaaattac ccgattgttt gaattggcta tcgaagaaaa
     9481 cctgccggtg gtgattttta cggcttcagg aggcgcccgt atgcaagagg ggatcatgag
     9541 tcttatgcaa atggccaagg tctctgcagc tgtgaaaaga cattctaatg caggcctctt
     9601 ttacctcact attttgacag atccaactac tggtggggta acagctagtt ttgccatgga
     9661 aggggatatt attttagcag aacctcaatc cttggttgga tttgctggac gccgtgttat
     9721 tgaaaccaca gtacgtgaga atttgccgga tgattttcaa aaagcagaat ttttacaaga
     9781 ccatggtttt gtggatgcta ttgtgaaacg aacagagttg cgagacaaga ttgcccactt
     9841 agtagcattt catggaggtg gccaatgaca gatgtatcaa gagttttaaa agaagcgcgt
     9901 gatcaagggc gtttaacaac tttggattac gccaacctta ttttcgatga ctttatggaa
     9961 ctgcatggcg atcgccattt ttcagatgat ggtgctattg taggtggctt agcttatttg
    10021 gcgggacaac ctgttacggt cattggtatt caaaaaggta agaatttaca ggataatttg
    10081 gcaaggaatt ttggccagcc caatccagaa ggttatcgta aagctttgcg ccttatgaaa
    10141 caggcagaaa aatttggacg acccgttgtt acgtttatca atactgcagg agcctatcca
    10201 ggtgtcggtg cggaagaacg aggacagggt gaggccattg ctaaaaattt gatggaaatg
    10261 agtgatctca aggttcccat tatcgccatc attattggtg aaggaggctc tggtggtgca
    10321 ttagccttag cggttgccga tcaggtctgg atgctagaaa acaccatgta tgcagtcctt
    10381 agtccagaag gctttgcttc tattttatgg aaagatggtt caagagcgac agaggctgct
    10441 gaattgatga aaattaccgc aggcgaattg tatcaaatgg gcatagtaga tcgtattatt
    10501 ccagaacatg gttatttttc aagtgaaatc gttgacatca tcaaagctaa cctcatcgaa
    10561 caaataacca gtttgcaagc taagccatta gaccaattat tagatgagcg ctaccaacgc
    10621 tttcgtaaat attaacaaca tccttttgga gtctccctac gtgtttatca agctaaggga
    10681 ggcctttcca aaagggtttt taactagtct tgtaaataag gaaatgggta tactaataag
    10741 acttttgata aactagtgtg aaaaaaagtg ttgttaaagg ggcaatgtag tgagtttcaa
    10801 gtgatgtggt ggctctttaa aatagtaaac cccagaaatc actttaatga tctctggggt
    10861 gagtttatta tttaggtgaa atgacggttt cgccacccat gtaagggcgg agtacttcag
    10921 ggatggtaac agaaccatct tcattttggt agttttctaa aatggctgct acggtacgac
    10981 cgacagcaag tcctgaccca ttaagggtgt gaagaagttt gaccttgccg tcagcttcat
    11041 cacggtaacg gatttgagca cgtcgtgctt ggaaatcttc tgtatttgaa catgatgaaa
    11101 tttcacgata ggtattttgt gcaggaatcc agacttcaag gtcataggtt ttagcagctg
    11161 agaagcccat gtctccagta caaagggaaa tgacgcgata tggcaaacca agtttttgaa
    11221 gaatattttc agcattagca gtcatttttt ctagttcttg gtaagattct tctggtttag
    11281 caaatttaac catttcaact ttgtggaatt ggtgtaggcg gataaggcca cgtgtgtcac
    11341 gacccgcaga acccgcttca gaacgaaaag atggactcat tgcagtaaag taaattggca
    11401 attctttgcc gtctaggatt tccccacggt aataattggt caaaggcact tcagcagttg
    11461 gaatcaggac aaagttggta tctgctaatt caaaggtgtc ctctttgaat tttggatatt
    11521 gtcctgtacc aaacatggaa tcgtggttaa ccatgtaagg tgtgatgatt tcttgataac
    11581 cttctttgat atgttcatcc agcatgaagt tataaagagc acgctccaaa cgagcaccaa
    11641 gatttttata gaataagaaa cgagcaccag ttacctttgc cccacgttcc cagtctaaaa
    11701 tatcaagatc ttcgcctaga tcccaatgag ccttaacttc gaagtcaaag tcacgaggcg
    11761 ttccccaacg gcgaatttcg acattgtctt cttcgtcagc tccaacaggg acagaatcgt
    11821 gaggagtatt aggtaaaaca gtgatgatgt cagtaacttg ctggtcgata gctactagtt
    11881 gattgtcaat agttttgata tctgcagaga ctttttgcat atcagcaatt tgttgagtgg
    11941 catcttcttt ttgacgtttg gcttgagcga tggcagcaga agcaatatta cgctctgctt
    12001 ttaattcttc tgattgcact aatagagtac gacgtttttc atcaagttct ttaagatggg
    12061 tcagggtgtc ttcagaaaca ccacggtttt ttaatttggc agcgacagtg tcaaaatcgg
    12121 tacggatacg tttaagatct agcatacata agctccttta agatagttat tagtagctgt
    12181 cagtgaaaca aggctaagta agacagttgg atcttgtgat tctctgacga tagaaaaagc
    12241 acagcttatg aagatgctgg agcgctttgg cgcggttcca tccagcttca caatctgtgc
    12301 acttaatctc tattttattg atgacacggt agaatttcat atcattttcc ctatctgttc
    12361 acagcatcca cagactctct gaatgaaaaa aaggtattac ttatccgtta ggactattat
    12421 aacaaaaaac aagaaaaagt aaactcaaat gagggaatct tttccactaa atagcaattt
    12481 agagctacta ttctttgttt tcttgaaatg ataaatcggc actgctcatt atttttttaa
    12541 tgtcgaaaaa atttgttaat aaaggtttgc atcaaggttg gtaatgtaat cactttctca
    12601 ttaccaatat gttgacgagt gatttttaag atttttccag agtctccaga agcaaataaa
    12661 aacttgcctt ggtccgtaaa aatttcaaag tggcggctaa ctttttttct tgaaacatta
    12721 gccccgatgt gattgatagc tgtccaagga atttgaatat aatcattcac atttttatcg
    12781 ttgtaaaatt caagggcctt gtcaccgagt aaaatatgac caactttgcc tcccagtcca
    12841 aggtaagaga cagcttttgt cttgtattcg acactagtgt ttaaagattg agccatttaa
    12901 gttcctttca ttgcgtagtt gtagttatta taccatattt taagtgggat tttcctgaca
    12961 taaagcacaa aaaagaagcc tgagttcatc tcagacttcc attggttgat tacataatac
    13021 ctgcaaggtg agcaaggata ccaaatgcaa acaagccgat gatgattgcg attggagaaa
    13081 cttttttctt caataaccac atgcaaagga atgtaaggag taatccagct aaacctggga
    13141 taagagagtc caattgtcct tgaagggttt gagcttggac tttatcaagg ctcataccgc
    13201 caatagcgtt ggcaaggata gttttcaact cagcaccttt aacagcgcca tctgggaaga
    13261 ccacataggc accatctgac aattgttttc caggaaggtc gatagtaaag ttgatagata
    13321 cccaacgttg aacaagcact gcaaggataa acattccaag gattgaagca cctttagtaa
    13381 tgtcttggag gataccgcct gacatgtctt tagtaatttc agaaccagct ttgtaaccaa
    13441 attcttgagt ataccataag aaggccatac ggataaggtt ccatccaaag aagaacagaa
    13501 gtggaccaac gatgttacct gttgaagcca aagaagcacc aagtgcccca aggataggac
    13561 gaaccgtaaa ccagaatact gggtcaccga taccagcaag aggtcccatc ataccgattt
    13621 taaccccttg gatagccttg tcatcaattg gcgtaccatt tgcgcgttct tcttcaaggg
    13681 caagtgttac accaatgatt ggagctgcaa cgtatgggtg agtgttaaag aattccatgt
    13741 ggcgctcaag agcagctgca cggtcttcag gagacgtata caatttttta agagcaggga
    13801 taagtgcata agcccagccc atgttttgca tacgttcgta gttccaagaa ccttgtaaga
    13861 attgtgaacg ccaccaaaca cgttgacggt ctgatttagt taatttaatt tgttcagtca
    13921 tttgttctta cccctttcta gtagtcttct aagatatcgc cgattggatc agctgaacct
    13981 gctgcattac caccgttacc acctttttta gaaagattaa ggtagatgaa ggcaattgct
    14041 acaccgataa cacccatagc gataagggtt aattggctaa tagctgcaag agcaaaacct
    14101 agggcgaaga atggccatac ttcacgagta gccatcatgt tgataaccat agcataacct
    14161 acagcaacaa ccatcgcacc accgatttgc ataccttcgt tcaaccagtt tggcatagca
    14221 ttcaaaacag attgtacggc tgaagtagga acagcaacaa gaagagctgc tgggacagcg
    14281 atacgcaatc cttgaagaag aagggcaata aagtggaagc gttcaatacc tgcgaaatta
    14341 ccttcagcgg cagctttgtc accagcgtgt acaagggcag ttgacaaagt acgaacgatc
    14401 attgtaagga aaagaccagc aacagcaagt gggatagctg ttgagtaagc aaaggtaatt
    14461 cctttttgag taaagtcgcc acttttgacc atgatgatag cagctgcaac agaggcaagg
    14521 gcagcatcag gggcaacggc agcaccgatg tttgcccaac caagagcaag catttgaagt
    14581 gtaccaccaa ggatgacacc agcttcaagg tgtccagtta caagtccaat caaagtacag
    14641 gcaacaagtg gttggtgcat ttgaaattgg tcgaggatac cttcaagacc agcgaagaag
    14701 gcaatgataa cgaccaaaat tgcagaaata atcgaaatat ctgacatgtt gtaatccttt
    14761 caaagtatag taaacgagga ctttttgcta ataagttagc tagtcatcgt tttaaaacct
    14821 tatagcgttt gaaacttatt taatgttagc tttttggata agctcaaata agtttttctt
    14881 agaatcgtta ggaactttac ggacgtcgaa tgtaacacct aggtcgcgta atttttcaaa
    14941 agtagcaacg tcttctttgt ccattgataa aacgttgtta accattgttt taccagttga
    15001 gtgagccatt gaaccaacgt tcaattcgtt gatttcaaca ccaccttcaa ccgcgcgaag
    15061 ggcatcttga ggggtttgga aaagaataag tgcgtgtgtg ttaccaaaac gaggatcttt
    15121 agaagcttca atcaatttgc taattggaac aacgttagct ttgacaccac caggtgcagc
    15181 ctgtttgatc aattgtttgc gaaggtcatc ttgagcaacc tcatcagaag caacaatgat
    15241 acggtcagcc ttagaagctg gagtccatgc tgttgcaact tgaccgtgta aaagacgagt
    15301 gtcaacacga gcaagattga tctttaattt accatctccg atgacagttc cagcaggaat
    15361 agctccttgg agtgcgttaa caactttttc agttgcagca gtttcttcaa ctgggttaag
    15421 gtcttcagga agggctttga taccgtcctt agattcctta atgatgtttg cagcaacttg
    15481 ttcaacacct gcaccagcat ccataagacg ttcagtgtaa gcttgaataa gcataggcaa
    15541 gttgagacca gtgatgatcg ccattttgcg gtcaggattt tcacctgcca cacgactagc
    15601 ttggttaaat ggagaaccac tccaaaggtc agctagcaca aggatttcat catcagcatc
    15661 aaattgttgg atagcattgt tgaagtgtcc atacaaatca tctggtcctt cgtttggcat
    15721 gaaagtaaca acttgaactt tctcttgttc accaaaaatc atagaaccag attgatgaat
    15781 accttcagca aatttaccat ggctggcaat aataataccg atacccattc ttgttcttcc
    15841 tcctttaaaa tatttttcgt tgaaatgaag aagaataatc tccttccaaa actaaatttt
    15901 ggcaattctt tttcacttaa acgaaattca agaacgtttc tattttaaaa cgttttcact
    15961 aaaaaatcaa gccctttcgg aaaaatagta caaaacatga a
//
LOCUS       DAUELZ010000037        15737 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.037, whole genome shotgun sequence.
ACCESSION   DAUELZ010000037 DAUELZ010000000
VERSION     DAUELZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15737
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.037"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            156..1388
                     /locus_tag="VHI09_000896"
     CDS             156..1388
                     /locus_tag="VHI09_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ6120782.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFAGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSSEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1556..2737
                     /locus_tag="VHI09_000897"
     CDS             1556..2737
                     /locus_tag="VHI09_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ6120783.1"
                     /translation="MTKRIILTIFTFICFSMMPLVHADDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNYNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(2889..4307)
                     /gene="sufB"
                     /locus_tag="VHI09_000898"
     CDS             complement(2889..4307)
                     /gene="sufB"
                     /locus_tag="VHI09_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEQ6120784.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4300..4779)
                     /locus_tag="VHI09_000899"
     CDS             complement(4300..4779)
                     /locus_tag="VHI09_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEQ6120785.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVRLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(4766..5992)
                     /locus_tag="VHI09_000900"
     CDS             complement(4766..5992)
                     /locus_tag="VHI09_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ6120786.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQHVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLTNKLTT
                     KTRFVSLVHVSSVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQSSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(6023..7285)
                     /gene="sufD"
                     /locus_tag="VHI09_000901"
     CDS             complement(6023..7285)
                     /gene="sufD"
                     /locus_tag="VHI09_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEQ6120787.1"
                     /translation="MTKEKLVAFSQAHAEPAWLQERRLAALEAIPNLELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKTSANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWTLAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQETAERLVIRGF
                     LGAVIAEIPIPSVRQEIIKVLDEKLLNR"
     gene            complement(7380..8150)
                     /gene="sufC"
                     /locus_tag="VHI09_000902"
     CDS             complement(7380..8150)
                     /gene="sufC"
                     /locus_tag="VHI09_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEQ6120788.1"
                     /translation="MSILEINNLYVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAVIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADEDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYITPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8272..9441)
                     /locus_tag="VHI09_000903"
     CDS             complement(8272..9441)
                     /locus_tag="VHI09_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEQ6120789.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLIAAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLILGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9448..10209)
                     /gene="mecA"
                     /locus_tag="VHI09_000904"
     CDS             complement(9448..10209)
                     /gene="mecA"
                     /locus_tag="VHI09_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEQ6120790.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHDSEPLDYIH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10355..11194)
                     /locus_tag="VHI09_000905"
     CDS             complement(10355..11194)
                     /locus_tag="VHI09_000905"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEQ6120791.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKQNAQQEPAVTDLARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTDYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11260..13185)
                     /locus_tag="VHI09_000906"
                     /pseudo
     CDS             complement(11260..13185)
                     /locus_tag="VHI09_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            13383..14951
                     /locus_tag="VHI09_000907"
     CDS             13383..14951
                     /locus_tag="VHI09_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ6120792.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKVAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSSTKERRNEISFSNSYYTSQPVLVVTANGKYADATSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGVIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKAS
                     FLGQMWAILKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRHIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            14944..15684
                     /locus_tag="VHI09_000908"
     CDS             14944..15684
                     /locus_tag="VHI09_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120793.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRPQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDRIIFMDKGL
                     IAEEGSPQQIFENPTQDRTKEFLQRFLK"
BASE COUNT         4861 a         3380 c         2816 g         4680 t
ORIGIN      
        1 tcctttttta atgaggtttc aattagttta ataaagattg tcattctctc atggtagcgt
       61 tttattacat cactactctt gtgaggtaag tcattacctt agacaaaaat atggtataat
      121 taaaaaaata atacttgagt aaggataatc tataaatgat caaacgatta atttccctag
      181 tggtcatcgc cttatttttt gcagcaagca ctgttagcgg tgaagagtat tcggtaactg
      241 ctaagcatgc gattgccgtt gaccttgaaa gtggcaaagt tttatacgaa aaagatgcta
      301 aagaagttgt cccagtcgcc tcagtcagta agctcttgac aacctatctg gtttacaaag
      361 aagtttctaa gggcaagcta aattgggata gtcctgtaac tatttctaac tacccttatg
      421 aactcactac aaactatact attagtaacg ttcctcttga taagagaaaa tataccgtta
      481 aagaactttt aagtgcgtta gttgttaata acgccaatag ccccgctatt gctttagctg
      541 aaaaaatagg cggaaccgaa cccaaatttg ttgacaaaat gaaaaaacaa ttaagacaat
      601 ggggtatttc cgatgcaaag gtcgtcaatt caactggctt aactaaccat tttttaggag
      661 ctaatactta tcctaataca gaaccagatg atgaaaattg tttttgcgcc actgatttag
      721 ctattattgc caggcatctc ttattagaat ttccagaagt actgaaatta tctagcaaat
      781 cctccactat ttttgctgga caaaccattt acagttataa ttacatgctt aaaggcatgc
      841 cttgttatcg agaaggcgtg gatggtctct ttgtcggtta ttctaaaaaa gccggtgctt
      901 cttttgtagc tactagtgtc gaaaatcaaa tgagggttat tacagtagtt ttaaatgctg
      961 atcaaagcca cgaggatgat ttagctatat ttaaaacaac caatcaattg ttgcagtacc
     1021 ttttaattaa ttttcaaaaa gtccagttaa ttgaaaataa taaaccagta aaaacgttat
     1081 ctgtcttaga cagttctgaa aaaactgtca aacttgtagc ccaaaatagt ttatttttta
     1141 tcaaaccaat acatacaaag accaaaaata ccgtccatat tactaagaaa tcatccacaa
     1201 tgatcgcacc tctatcaaag ggacaagtct taggtagagc aacccttcaa gacaaacatc
     1261 ttattggaca aggttatctg gatactcctc cttctatcaa tcttatcctt caaaaaaaca
     1321 tttctaaaag tttcttttta aaggtctggt ggaaccgttt tgtgaggtat gtcaatacct
     1381 ctttatagaa taacacaatg ctttttgtat gacattgact gacagagtac ttctgcagtc
     1441 tttttcattt ttatttaagc aaactaggcc aatcctaaca atttttcaaa aaaacttcac
     1501 attgtattag caaagtgata tactgaaaat gcattacatt taaaggagtc ggaaattgac
     1561 caaaagaatt atactaacta tttttacctt tatttgcttt tcgatgatgc ctcttgtcca
     1621 cgccgacgat gtcatggata ttacaaggca agcaggctac acagttagtg aggttaatcg
     1681 accaaaatct tctattgtcg ttgatgcaaa tagttcagat attctttggc aagataatat
     1741 tgatattcct agggatcctg ctagtatgtc taaaatgttt acgctttaca ttttatttga
     1801 agagctcgct aagggaaaaa ttacgatgga cactactatt acggcaactc caaccgatca
     1861 agctatcgct aacatttatg agattagcaa taataatatc gtcgctggag tagcttatcc
     1921 tattcgtgat cttattacta tgacagctgt gccttcatca aatgcagcaa ctgttatgat
     1981 tgccaattat ttatcaaaca atgatgcttc tgcgtttatc gatcgtatta atgccacagc
     2041 caaacaatta ggcatgacta acactcattt ttcaaacgct agtggtgcag cagcacaagc
     2101 ttttcaaggt tattataatc ctactaaata tgatttatct gcttcaaata ttacgaccgc
     2161 tcgagatttg tccaagttac tctatgcctt cttaaaaaaa taccctgaaa ttatctcctt
     2221 tacaaataaa tctgttgtgc acactatggt aggaacgcca tatgaagaag aatttcacac
     2281 ctataaccac tctctcccag ataatcaatt tggtatgaaa ggagttgatg gattaaaaac
     2341 aggttctagt cccagtgctg cttttaacgc catgataact gccaaaagag gcaaaactcg
     2401 actcattact attgtcatgg gggtcggaga ttggtcagat caaaacggag agttttatag
     2461 acatcctttc gttaatgcct tgactgagaa agggtttaaa gatagtaaga ccttgtccaa
     2521 aaaagcacgc caaaaactcg aaaaattagt gccacaaacc aaaaaagaaa catcacctaa
     2581 acagcagcac ttcaaagcca ctaaaaagca atcgtacttg gaaagagtcg aagattttat
     2641 gaattacaac catactttct tgcttatttg cttagctatc tttataatta ctatcctttt
     2701 actaagcctc gttgtctttg caatgggacg ccaataatga attatcaaaa acctctgtca
     2761 aagcacagag gtttttgata tgttatctta cgtgtttaag gcaactaaaa actaaccact
     2821 tggggttagt actacaactc ataaaatcct atctcaaaga tctttcagat ggataagaga
     2881 aaatgcaatt aaccgacaga accttccatc tcataggaaa tcaatcgatt gagttcgacc
     2941 gcgtattcca ttggcaattc tttggtaaaa ggctccacaa atcccatgac aatcatctct
     3001 gtggcttcgc tttctgataa gccacggctc atgagatagt agagttgttc ttcagaaatc
     3061 ttagacactt tggcttcatg ttctaacgcc acctgtgaat tatgaatttc gttaaatggg
     3121 atcgtatctg atttagaaat atcgtccatc aaaatagtat cacattcaat atgggaaacg
     3181 gattttttag attgcttgtt aaaggtcact tggccacgat aatctacttt accaccagac
     3241 ttggcaattg attttgacac aattgatgat gatgtatgtg gagcattgtg aatcatttta
     3301 gcccctgtat cttggtgttg gcctgcgtta gcaaaggcaa tagacagcat ggtgccacgc
     3361 gcacctggcc catcaagata aacagaaggg tacttcatgg tggttttagc tcctagattg
     3421 ccatcaatcc attccactgt tgcatccgta agggcgcgtg cacgttttgt cactagatta
     3481 tacacattgt ctgaccagtt ttgaatggtg gtataacgca tgtatgcacc gtcaagcgca
     3541 aagatttcaa cgatagcagc atgtaagctg ttacttgaat aagtgggagc tgtacatcct
     3601 tcaacataat gaacacttgc tccttcatca acaataatca atgtccgctc aaattgacca
     3661 gtattttcat tgttaatgcg gaaataagtt tgcaaaggga tatctacctt gaccccttta
     3721 ggaacataaa taaaggtacc accagaccaa actgctgaat tgagggctgc taatttgttg
     3781 tctgttggcg gaaccagttt ggcaaagtat tgtttgaaca aatcaggata ttctttgagg
     3841 gcggaatcgg tatctgtaaa gataatccct agcttttcaa attcaccctt catgttatgg
     3901 taaaccactt ctgactcata ctgagctgat gctccagcaa gataagcacg ttctgcttct
     3961 ggaattccaa tacgatcaaa ggtttctttt attttttctg gaacatcatc ccaagaacga
     4021 gctggcttat cagatgcttt ttgatagtaa atgatatcat caaagttaat atctgataag
     4081 tctgctcccc aggtttgcat cggcatttta ttaaaggttt ccaaggattt taaacgaaac
     4141 tccaacatcc actcaggttc atttttggca gctgatagtt cgcgaaccac tgcctcactc
     4201 aatccttttc ctgtagaata aatgggctct acatcatcgt gaaatccaaa ttggtagtcc
     4261 cctaagtcaa ttggctttgg ctctactttc tcatttatat cagacatttt tcccttcctt
     4321 tacattttgg tccgtgaggt gctgagcatt ggcacttcgt ttaatggctt ccttgagagc
     4381 attccaagct agggtagagc atttgatacg ctgtggaaat tttgcaactc ctgccaacaa
     4441 ttctgcttca cctagctctt tttgagcagg attttcctgt ccttgtacca tctctgaaaa
     4501 aatatcagct aacgcgagtg cttcttcttt acttttacca ataacagcat ctgtcatcat
     4561 gcttgatgaa gctgtggaaa tggtacagcc gttgcctgca aaagcaatat cttcaatttt
     4621 atcttcgtca aacttaacgg tcaaagaaat cacatcacca caagtcggat tattcagtcg
     4681 aacagcctct acgccatcta gttgcccatg atgatgtgga cgtttcgaat ggtccgctac
     4741 cacagccatg tatagatggt tcagtttaga gagtgccatt gaaaaactcc tttgctttta
     4801 gaattgcttc gactagacgg tcacaatctg ccttggtgtt atagatataa aaacttgctc
     4861 taacagttgc tggtacacct aaataactaa gaagaggttg ggcgcagtgg tgccctgctc
     4921 ttactgcaac accttcatag tccaaggctg ttgccaagtc atgaggatgc aaatcatcca
     4981 gattaaaaga aatcagacca gatcttgcac tagactggct tggtccatat atggtaagcc
     5041 cctcaatagc ttctaatttc ggcaaaacat aggctattag ttctgcttca tgcgcatgta
     5101 tatcagccat gcctagtctc tgaaggtaag aaatggctgc gcttagccca atagcaccag
     5161 ctatgtgagg tgttcctgct tcaaacttcc agggcaattc tttccaagtg gcctcttgtt
     5221 cgtaaacaaa atcaatcatt tctccgccaa attcaagagg ctccacttga ttcaaaagct
     5281 cttctttgcc gtaaagaaca cccaaacctg ttggccccaa catcttatga gctgaaaaag
     5341 caaagaaatc acaatccaag tcttgaacat caatagccaa atgtggaacc gactgggcac
     5401 cgtcaacaac aaggtaggct cctttagcat gtgccagctt ggcaatttct ttaatgggat
     5461 tgatgcaacc aagaacactg gagacatgta ctaggctaac aaaacgtgtt tttgtcgtca
     5521 atttgtttgt caaatcgtcc atgtcaagtt ggccatcttt taaataaaca tagactaacc
     5581 ttgctcctgt tttttgacag gcttgttgcc aaggaatgat attggcatga tgctccataa
     5641 tcgaaatcaa cacctcatct tctggcgtca agacctgctc tgcaaaccga gcaacccaat
     5701 tcaaactggt tgttgttcct ctggtaaaga gcacttcctt gcttgattta gcatgaataa
     5761 agtcagcaac gtgctggcga ctggcctcat atttgtgcgt tgcacgttca gccaaggtat
     5821 gaactcctcg gtgaacatta gcattatctt cttgataata ggattgcaaa gcctcaagaa
     5881 caagcgccgg tttttgtgtg gtggcggcat tatccaaata aacaaggggt tcatcattga
     5941 cttgttgatt taagatttga aagtcttgtt tgatgtcttt tgcgtctagc aatactaacc
     6001 tctttctttt tggcagtagg tcttaacgat taagcaattt ctcatctaaa accttaataa
     6061 tctcttggcg gactgatgga ataggaattt cagcgataac cgctcctagg aatcctctaa
     6121 taaccaatcg ttctgctgtt tcttgatcca gtcctcgact catcaagtaa tacatatctt
     6181 cagggtcaac ctgaccgata gaagctgcat gacctgctgt tacttcattt tcatcaatta
     6241 agaggattgg attggcatcg gctcttgctt ggtcagaaag catcaaaaca cggctttctt
     6301 gttgagcatc agctccctta gcgcctttta gaatatgacc aatcccgtta aacgttaagg
     6361 tgccacgttc caaaatcaca ccatgctgta aaatatgacc gaccgtacgt tgaccatagt
     6421 tggtcacgcg cgtgtcaata ccttgtacct gacgaccact tgaggctgca acaactttca
     6481 aatcagcttg tgagccctga ccaatcaaat cactgtcaaa atcagcaatg acattgcctt
     6541 cattcatcac agctaaggtc caatcaatgt tggcatcctt ctctaaacgt cctcgacggc
     6601 taatataggt tgtcactgaa ggacctaagc ggtcgatagc cgagaattta atctggctgc
     6661 ctgcttgagc aatcacttct acactgatat tagcgctggt cttttgagtg gcattgccaa
     6721 tagattcaaa acgctctaaa taggtgaact tactttcttt tcctgcaatc actagaacat
     6781 gcttgttaaa aggaacgtca ctgtcactat cttgtaagaa aatagcttca ataggagttg
     6841 tgatttccaa gtgatcagga acgtagagca cggctgcgct attaaaataa gcagtgtggt
     6901 aggcagctag tttgtcttca tcaaaagcta atgcctgacc aaaatgagct tcaattactt
     6961 ctgggatttc ctcaagcgcc gtataaaaat cactgaaaac aactcccttg tcaattaacg
     7021 ccattggtaa ctgttctaag actgtttgcg tgcctacctg aacaagcttt gggttatctc
     7081 caatagctat aaaatctgga acactagcta gactttcatt ttctgttaag gtaccatctc
     7141 ctagattcca acggtgaaat ttaacccttt cgatggttgg taattccaaa tttggaatgg
     7201 cttctaatgc cgctaaacgc cgttcttgca gccaagcagg ctcagcgtgg gcttgcgaaa
     7261 aagccactag tttttctttt gtcatctgtc aatcctttgt ctctggtttt taaattggct
     7321 gggtatatag aacatcacct agctttttgt agaaagctaa agtaattgtc tccaaaaact
     7381 taactttctt ccttatactc gattcccaga tcctgtgcaa tacctgcgta gccttctttt
     7441 tcaagacgag tagccaaggc agcatcacca gataagacaa tacgaccatc catcataaca
     7501 tgaactaggt ctggggtaat gtaattcaac aaacgttgat agtgggtgat aatcatagct
     7561 ccaaagtctt tcccacgcat ctcattaacc cctttagaga ctacttttag ggcatcaata
     7621 tcaagaccag aatcaatttc atctagcaaa gcaaatttag gctctaacat gagcaattga
     7681 agaatttcat tgcgtttttt ctccccacca gaaaaacctt cattgaggta acgctctgcc
     7741 atctcttctt tcatacctaa caaagccatt ttctcatcaa gtttggtgat aaaatctcgc
     7801 acggaaatct tatcctcatc tgccttaccg gcattcattg ctgcacgcat aaattcagcg
     7861 ttggtaatcc ctggaatttc tgaagggtat tgcatggcga gaaaaagtcc cagtctcgca
     7921 cgctcatcaa cttctagatc gagaatattg accccgtcaa gcaaaatctg cccttgggtc
     7981 acttcgtagt taggatttcc cataataact gctgaaaggg tagactttcc tgtcccattt
     8041 ggccccataa tggcagctac ttccccagtt ttaagagtta ggttaacccc tttcaaaatc
     8101 tctttacctt ctattgaaac atataaatta ttaatctcaa gaattgacat gatacacccc
     8161 ttattttctc tgtcatctat accatctcaa gaaaagacag tgtcatttgc actgcttttt
     8221 ttataatggt cactgcatct attatagcaa aaaaagcctt actcggcttt tttagtgttt
     8281 caaatccttc ttttctttcc atttcagaag cattgcttga cgataatcac tattaccaat
     8341 aaactttaac aagttaaaca aaggcgttcg cttttcaccc cagatttcca atccttcaat
     8401 aaaaacttct aaaccaaata aaagtccaag gatcaagagg acgccaccaa ttcgactaga
     8461 aacatttaaa agtaaagaaa taagggaaaa aagcatggta ataccataaa ctactaagac
     8521 agctcctcga tgagtaaagc ccattgataa gagtcgatga tgcaggtgca tcttatccgg
     8581 ctcgtagaat ttttgacctg ataaactacg tcgaataata gccacgatgg tatccataat
     8641 gggtacacca agaataataa caggtgtcac cacagccaca gccgttgaat ttttcaatcc
     8701 ttgaagagac aaaaccccta tcataaagcc aataaagagg gctcctgtgt cacccaaata
     8761 aatcatagct ggatggtaat tataaggaaa aaatcctgca atagcagcaa tcaagaccaa
     8821 aatggttaac gtcaaaaaga aatctttttg aggtaaaaag aaataagata caatagccat
     8881 ggtcactaaa ctaataatag agaccccact aaccaaacca tccaaaccgt caatcaagtt
     8941 aatagcatta gtgatggaaa caatccacag gactgttaag aataaggtta agaaaggacc
     9001 aaaaactaac aacggccctc caaaaggaat tttgaagcta tcaaatttaa agtcggtaaa
     9061 agcccagaca atcactgcac caattagaat accaaacatc tttaatttag gacttaactc
     9121 atacaaatca tctaaaaaac cagtaagggt aattactgta gcaccaatca cgacaggtaa
     9181 aatataactg aagtaggtct gaccaaaaaa catggcgcct ttagaagcaa tcggaataag
     9241 tcccaaactt gctactaaaa aagacataaa aatagctaat ccaccactgg ttggcatcgg
     9301 aaccttattg acccgtctgg cattgggatt gtctactgca cccacacgaa aggctaaaaa
     9361 gcgaacaaga ggggttaaga ataaggacat taacaaagcc cctatcagaa caaggacata
     9421 atctattgtg aatgaaaaca tactatttta tcccaactca atcttttgta attgctcaac
     9481 agcaccatca agcatcaatt gtaagccatg ctcttgtaaa taagctcttg tttttgatcc
     9541 agggtttgca tgctcaatca atctcgcata catgacattc gcaaaataag atggttgttg
     9601 ctgaacatct aataaaatag tcatatggta gcagttgctc cccttatata actctgaagc
     9661 ttctatcgaa aaatcaatcg ttttagcaaa agccactgcc tctgtaatcg ttgaaaaatc
     9721 caatacataa tggatatagt ctaaaggttc tgaatcatga ttagttgatg gatcagccgc
     9781 ttcagactga ttagctagag tggcttcaac aacatcttcc attatctctt caattttttc
     9841 caatttttca tgagctttga catctccctt ttcacgcatg gattgctcca agctcttgaa
     9901 aaaatcttcc ggagtcattt gggacatatc accaaactct gctaaatctt caagattaat
     9961 atctttattt atctcagatt tggtaacaaa aacatctaga cggtccttgc gtggagtcac
    10021 tcgaaaacta agcataccac tatctttaaa attatctgga agatcgagtt cgtccatcac
    10081 agaatagaaa aactcttctg ttttttcttg aggaatcaag aaatctttca actccattcc
    10141 tctttcttct aaatcgtcca tactaattgt tatttttagc gttgtctcgc taatttgttt
    10201 catttccatt atcaatacct cgcacgcgta gtcattttat tatactagaa atgctgtaat
    10261 ttcgcaattt atgactaact cctcttctac aaaaggaaat ttaaaaagcg acagtcccca
    10321 aggtctgtcg caacatccta aacgatatgt aagtttaaaa aacaaattta aagaagctat
    10381 aaataagtaa aaggcttcct aacacaatac ggtatttccc aaaaatggtg aaatcgtgtt
    10441 tcttaacata atctgtcaag aacctaatcg ccaataaact aaccacaaaa gcggtcaagc
    10501 tagccactaa aagaataagg acttgtgcaa aatcaaggtg atggccatca aggaaaaatt
    10561 tcacagcctt taatccacta tatccaaaca tggtaggaat ggccaaaaag aaggtgaaat
    10621 cagcggccac tgttcgacta gttcctaaaa taatagctcc taagatggta gcccctgatc
    10681 gactagtacc aggcacaata cttaagactt gaaaacaacc aatgaagaaa gctgttttgt
    10741 aagacattct agctaagtca gtgacggcag gttcttgttg agcattttgt ttttcaatcc
    10801 agataaaggc aatcccgtag acgattaaag cgatggcaat cggaaccata aaataaaaat
    10861 gtgcttcaaa ccaattatca agaggaaccg ctattaaaat agaaggaata cacgcaatca
    10921 ccacttttag ccataattgc caggtaagct gtacttctct tgctgttttc ccaggttgga
    10981 agggattgag acgttcaaaa taaatcaaca taacagcgat aatcgcccct agttgtataa
    11041 cgatattaaa catttctata aaagccttat cctgatttag tcggataaat tcctgcacta
    11101 aaatgaggtg accagtgctt gaaactggta accattctgt gattccctca atgatgccaa
    11161 agaaaatggc ttttagcaat tcgattatta acatgttttc tccttattaa ccactcttat
    11221 tgtgattttc taaactgtga ttaatgccaa gcagcttttt taaaaggcac cgccgccgcc
    11281 tcctccgcca ccgccagaga agccgccacc accactaatc ccgcctccgg aagagactga
    11341 gaaatttgag gaagttgtag ctgaagacaa gctagacaca aaggttagtg tcgtagcata
    11401 cattaccatt gataattcac ctggtcgaac agcttggtaa acctcaggta aggcaatacg
    11461 atggactttc aaatagcgtt ccactttctt agcgtaacca aataaggtgg cataaaccaa
    11521 aacacggttc caaacaacca aaccttcaat agcaacatcc tcaaatttgt caatgtctcg
    11581 tatcatgttt ttaaagctct gctattgatg taaccgaaca cccccctcag gtgtttcaat
    11641 acccaatttc ttgtattgat ctaacttcag gctaatgtaa aagccaaggc acatcgttag
    11701 caccattaaa gcaaggtaaa tgagggccaa cccacttcct ttcatagtca gatagatcaa
    11761 taaactacta ttcagaacaa ctaacaagca gcctaacccc tggacactat gactttttcg
    11821 tttctctgcc ggggtcattt gacgataagt atctggcaag tttaactttt tgatagtttc
    11881 taggacattg tttgtaatag ttgccgacgc ttttttcatc gccttaatca cctgctcaga
    11941 cgattgacgg acttcttgct cgagcttttt acctttgtac gttttcttta attgtttaat
    12001 ggtatcagca tcataatggt attgactaaa caattgatcc accggcaatg tcaccttgtt
    12061 tccaaaagcc atatctaaga aggcagcttc ctcatctgtc acacgatcta actgactgat
    12121 ttcaagataa gctttccctt cttctttcgt taataaaagc accttttggt cgatcagatc
    12181 caacaaggta gcttgaacaa gggcctcaaa ggtcactcct ttagggatca ataatttctg
    12241 actttctgat gctgtagggg ataaataggc aaaactctgt ccataaatcg cttgtgtcaa
    12301 cacaagaggc gaaagatctt cagggacttc gtaactgtga tctgtatggt tggccaaatg
    12361 ataacgattg aattgtttct ttaatcttgt aaactgtatt aactgccaaa gaagaaaacc
    12421 tacctctacc agaggaatga ctttgccaaa tagcagctgt aacaaggccc ttcgtctgga
    12481 aatcttagtt tctaacgcta caattttatt ctttttactc gttgaaacat caactggttc
    12541 ttctcccaat aatgcttttt tatcccaata agcatgcaat tccaattgcc ctgagacatt
    12601 tttggcatta atctgatagc gactgttacc ttcttttaac acttgaggtt tcttttggta
    12661 atatcctctg tgagcccata gattagaatc ctgaatatca gttggggtag taacggtcaa
    12721 actcaccttt tctaatgttt tgtcccagtc actgatcggt gtccaattca gctctgccac
    12781 gtcttgataa gctgttaaaa ggtggtgcag ttgccaggta accttaacgt caaccttatc
    12841 accagcttgg ccagcattat aaagttttaa acgatagcca tctccaaggt cactaaactc
    12901 ttgactaaca ggtacctgtt gaccattctg atatacctca actttaggct tttggtcaat
    12961 agcaaagccc gctggcaaat gacctgcccg gcccagcgaa atatactggc cgttataaga
    13021 agtgtcaaat tgataggtta ctttttgctc aaaccgagca gtattttctt ttgataacag
    13081 taactgacct tcatagttgg tgatgctata gtctacatca gctgctttta ttcgtatccc
    13141 taaaagactg aagcagagca caagcgtcat cagaattttt ttcatggtaa catcctcttt
    13201 ctgccctcat tttaccatat tttaaggaga tgacaaaacc taaaccaagt ccttcatctt
    13261 atttttaatc attattttga atatttttcc atatttcaac ctattcactg ataatatatt
    13321 gaaataggat gcaaattgct gtaaaatagg tctgaatact atttttgaaa caggagacct
    13381 atatgaaaaa gctaatcctt agctgtttgg tcgccttggc ccttctgttt ggaggaatga
    13441 gccgggctca agcaaaccaa tatttaagag tcggaatgga agcagcctat gctcctttta
    13501 actggactca agatgacgct tcaaacgggg ctgtccctat tgagggaact agccaatacg
    13561 ccaacggtta cgatgtccaa gtcgctaaaa aagtcgctaa agctatgaac aaagaactgt
    13621 tagtcgttaa gacctcttgg accggtttaa ttccagcatt aacttctgga aaaatcgata
    13681 tgatcgctgc tggtatgagt tctaccaaag agcgtagaaa cgaaattagc ttctcaaaca
    13741 gctactacac tagccaacct gttctagtcg taactgctaa tggcaaatat gctgatgcaa
    13801 caagcctcaa ggatttttct ggagctaaag taactgccca gcaaggcgtt tggcatgtca
    13861 atctcttaac tcaactaaaa ggtgctaagt tacaaacacc aatgggagat ttctctcaaa
    13921 tgcgacaagc ccttacttcg ggtgttatcg atgcctatat ttctgaacgg cctgaagcca
    13981 tgactgctga agctgctgat agccgtttga aaatgatcac tcttaaaaaa gggtttgctg
    14041 ttgctgaatc agatgctgct atcgctgtcg gaatgaaaaa aaatgacgat cgtatggcaa
    14101 ctgtcaacca agtgcttgaa ggattttctc aaacggatcg tatggccctg atggatgata
    14161 tggttaccaa acaacccgtg gaaaagaaag ccgaagatgc taaagcatca tttctaggcc
    14221 aaatgtgggc tattttaaaa ggtaactgga agcaattctt acgtggaact ggaatgaccc
    14281 ttctgatttc catggtcgga accattacag gtctctttat tggattatta atcggtattt
    14341 tccgtacagc tcctaaagct aagcataaag tagctgcctt gggacaaaaa ctctttggtt
    14401 ggttactcac tatttacatc gaaatcttcc gtgggacacc tatgattgtt caatctatgg
    14461 ttatttacta cggaacagcg caagcctttg gtatttcgat cgaccgtacc ctagcggcta
    14521 tttttatcgt atctatcaat acgggtgcct atatgagtga aattgttcgc ggtggtattt
    14581 tcgctgtcga caaaggtcaa ttcgaagcag caactgctct tggctttact cacggacaaa
    14641 ccatgcgtaa aatcgtgcta ccacaagttg ttcgcaacat tttaccagca acaggtaatg
    14701 agtttgtcat caatatcaaa gatacttctg tcttgaatgt tatctctgtt gtggaacttt
    14761 acttctcagg taataccgta gccacacaaa cctaccaata tttccaaacc tttacgatta
    14821 tcgccattat ctactttgtt cttaccttca cggtgacacg tatccttcgt catattgaac
    14881 gccgtttcga tgccgatact tacaccactg gagcaaacca aatgcagatt gcggaggtct
    14941 caaatgtcta actcaattat tgaaatcaaa aacctcaaaa aatcttacgg cagcaatgaa
    15001 gtactcaaag atatctccct ttctgttaac aagggagaag tcatttccat tatcggttct
    15061 tctggttctg gaaaatcaac cctcctgcgc tcgatcaatc ttttggaaga gccaagtgcc
    15121 ggtcaaatcc tctttcatgg tgaagatgtc cttgctgaac actacaactt gactcactac
    15181 cgtgaaaaac taggcatggt tttccaatct tttaacttgt ttgaaaacct aaatgtattg
    15241 gaaaatgcta tcgtcgctca aaccaccgtc ctcaaaagag accgtccaca agccgaacaa
    15301 atcgctaaag aaaacctcaa tgcggttggt atgacagaac aatactggca agccaaacca
    15361 aaacaattat caggggggca aaaacaacgg gttgccattg ccagagcctt atctgttaat
    15421 ccagaagcca tgctctttga tgaaccaaca tcagctcttg atcctgaaat ggttggtgag
    15481 gtccttaaaa ccatgcaaga cttggcaaaa tcagggttga ccatgattat tgttacccat
    15541 gaaatggaat ttgctcgtga tgtttctgac cgtatcatct tcatggataa gggattgatt
    15601 gctgaagaag gaagtcctca acaaattttc gagaatccta cacaagatcg taccaaagaa
    15661 tttttacaac gcttcttaaa ataagataaa ggatccaact atttctatgc cagttgggtc
    15721 ttttttcaat tcaaaaa
//
LOCUS       DAUELZ010000038        15732 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.038, whole genome shotgun sequence.
ACCESSION   DAUELZ010000038 DAUELZ010000000
VERSION     DAUELZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15732
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.038"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            15..1076
                     /locus_tag="VHI09_000909"
     CDS             15..1076
                     /locus_tag="VHI09_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEQ6120794.1"
                     /translation="MVQLVTKSNRPIGTAYISKQNKGIGWYLGPEKIDLSISYFVSLF
                     SVAKAKRQDFAQSDETNAYRLFNQEGDGFGGVTIDLYKDFAVFSWYNAFVYDKKEMIM
                     EAFQQVFPEVKGAYEKCRFKGPDTETAHLYGELAQETFSILENGIAYQVFLNEGLMTG
                     IFLDQHDVRRALVDGLAMGKSLLNLFSYTAAFSVAAAMGGAIETTSVDLAKRSRELSL
                     AHFEHNQLNLASHHFVVMDVFEYFKYAKRKKLIFDVIVIDPPSFARNKKQTFSVSRDY
                     HKLITEALDILSPKGTIIASTNAANMTVSQFKKQIIKGFGSRRPESMTLQQLPSDFTI
                     NKADERSNYLKVFTIKVRE"
     gene            1073..1759
                     /locus_tag="VHI09_000910"
     CDS             1073..1759
                     /locus_tag="VHI09_000910"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEQ6120795.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            1853..3019
                     /gene="aroC"
                     /locus_tag="VHI09_000911"
     CDS             1853..3019
                     /gene="aroC"
                     /locus_tag="VHI09_000911"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEQ6120796.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            3080..3421
                     /locus_tag="VHI09_000912"
     CDS             3080..3421
                     /locus_tag="VHI09_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEQ6120797.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADASASELFDE
                     FVAMQEKIQEMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            3643..4995
                     /gene="gorA"
                     /locus_tag="VHI09_000913"
     CDS             3643..4995
                     /gene="gorA"
                     /locus_tag="VHI09_000913"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEQ6120798.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKDKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFGLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(5083..6351)
                     /locus_tag="VHI09_000914"
     CDS             complement(5083..6351)
                     /locus_tag="VHI09_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ6120799.1"
                     /translation="MTTYDSISWIHSFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTISFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIETTTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLMQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISKVRQYCLSNANIFKNIFP"
     gene            complement(6381..6821)
                     /locus_tag="VHI09_000915"
     CDS             complement(6381..6821)
                     /locus_tag="VHI09_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120800.1"
                     /translation="MSSHYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITITRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTPETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSTTEGTQLPNATGAEDANTGLPTNP"
     gene            7056..8198
                     /locus_tag="VHI09_000916"
     CDS             7056..8198
                     /locus_tag="VHI09_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ6120801.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGS
                     LVAMGVPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            8210..9424
                     /gene="thiI"
                     /locus_tag="VHI09_000917"
     CDS             8210..9424
                     /gene="thiI"
                     /locus_tag="VHI09_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEQ6120802.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLSNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            9462..10754
                     /locus_tag="VHI09_000918"
     CDS             9462..10754
                     /locus_tag="VHI09_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEQ6120803.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     ATVVALIVALLLFGLIYDLLGVQKNELAAQKSAQPKVKAARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     gene            complement(10773..11657)
                     /locus_tag="VHI09_000919"
                     /pseudo
     CDS             complement(10773..11657)
                     /locus_tag="VHI09_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     misc_feature    11892..11966
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            12001..12315
                     /gene="rplU"
                     /locus_tag="VHI09_000920"
     CDS             12001..12315
                     /gene="rplU"
                     /locus_tag="VHI09_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEQ6120804.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            12327..12653
                     /locus_tag="VHI09_000921"
     CDS             12327..12653
                     /locus_tag="VHI09_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEQ6120805.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            12681..12974
                     /gene="rpmA"
                     /locus_tag="VHI09_000922"
     CDS             12681..12974
                     /gene="rpmA"
                     /locus_tag="VHI09_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEQ6120806.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            13332..14246
                     /locus_tag="VHI09_000923"
     CDS             13332..14246
                     /locus_tag="VHI09_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ6120807.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLSDIQGLPAVRFTQEGDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            14243..14701
                     /locus_tag="VHI09_000924"
     CDS             14243..14701
                     /locus_tag="VHI09_000924"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEQ6120808.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMVHLDFVDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            14691..15581
                     /locus_tag="VHI09_000925"
     CDS             14691..15581
                     /locus_tag="VHI09_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ6120809.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDTLAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
BASE COUNT         4780 a         2764 c         3429 g         4759 t
ORIGIN      
        1 aggaaaaaaa tcagttggtt caactggtga ccaagtccaa tcgtcccatt ggaacagcat
       61 atatctctaa gcaaaataaa ggaattggtt ggtatctagg accagaaaaa atcgacttgt
      121 ctatctctta ttttgttagt ctattttcag ttgctaaagc caaaagacaa gattttgctc
      181 aatcggacga gaccaatgct tatcgccttt ttaatcaaga aggagacggt tttggtggag
      241 taacaattga tctttataaa gattttgcag tcttttcatg gtacaatgcc tttgtctatg
      301 acaaaaaaga aatgattatg gaggcttttc aacaggtttt tcctgaagtc aaaggagctt
      361 atgaaaagtg tcgctttaaa ggtccagata ctgaaactgc tcatctgtat ggtgagctgg
      421 cacaagaaac cttttcgatt ttagaaaatg gcatagccta tcaagtcttc ttgaatgagg
      481 gcttaatgac aggtattttt ctagaccagc atgatgttag aagagcacta gtagatggtt
      541 tagctatggg caagtccctt cttaacctat tttcctatac agctgccttt tctgtagcag
      601 ctgctatggg tggtgctatc gagaccactt ctgtggactt ggctaaacga tcgagagaat
      661 tatcactagc tcattttgag cataaccagt taaacttagc ctcacatcat tttgtggtga
      721 tggatgtttt tgaatacttc aaatatgcca aacggaaaaa attaattttt gatgtgattg
      781 tgattgaccc accaagcttt gctagaaata aaaaacaaac tttctctgtg agcagggact
      841 accacaaatt aatcacagag gccttggaca tattgagtcc taagggaact attatagctt
      901 caacaaatgc tgctaatatg actgttagcc agttcaaaaa gcaaattatc aaaggatttg
      961 gaagtcgtcg tcctgagtct atgactctcc agcagcttcc gagtgacttt actatcaata
     1021 aagcagatga aagaagtaac tatttaaagg tatttactat aaaggtacga gaatgagaat
     1081 tgtagcacca gtcatgccaa gacattttga cgaggctcaa gccattgata tttctaaata
     1141 tgaagatgtt aatttaattg aatggcgagc agactttctt cccaaggatg agattgtcgc
     1201 agtagcacca gctatttttg aaaaatttgc cggaaaagaa attatcttta cccttcggac
     1261 cgttcaagaa ggggggaata ttaccctttc aagtcaggag tatgttgata ttatcaaaga
     1321 aatcaatgct atttataatc cagactatat tgactttgag tattttacgc acaagtcagt
     1381 atttcaagaa atgcttgatt ttccaaatct tattttgtct tatcacaact ttgaagagac
     1441 tcctgaaaat ttaatggaag ctttttcaga aatgaccaag ttggcaccgc gtgtggttaa
     1501 aattgctgtg atgccccaga gtgaacaaga tgttctagat ttgatgaact ataccagagg
     1561 ctttaagacg ctcaatcctg agcaagaatt tgcaactata tctatgggga aattaggaag
     1621 attgtctcgt tttgcgggag atgtcattgg ctcttcctgg acatacgttt cattagatca
     1681 tgttagcggt ccaggtcaag tgactctcaa tgatatgaaa cgtattattg aggtcttaga
     1741 aatggatatc tctaactagt gaatggtcta aaaatattat ttctacaata gttaagaaat
     1801 gatgttttta gattaatgtt tttaaaataa gtggaaccaa aaaggagaaa aattgcgtta
     1861 tttaacagca ggagaatctc atgggccatc cttaacagct attattgaag gtataccagc
     1921 aggattaact cttcatccag cggatattga tcacgaatta cagcgacgtc aaggaggtta
     1981 tggccgtggt gccagaatgt ctattgagac tgatcgggtt caaatatcct caggagttcg
     2041 tcatggcaaa acaacagggg cccccattac cttaacggta attaataagg accaccaaaa
     2101 atggctagat gtcatggctg taggagacat tgaagagact ctcaagttaa agcgtcgtgt
     2161 taagcatcct agaccaggac atgcggattt ggtaggaggt attaaatatc attttaacga
     2221 tttaagagat gctttagagc gttcctcggc tagagaaaca acgatgagag tggctgtagg
     2281 cgctgttgct aaacgtatct tagctgaact tggaattgat atgttacatc atatcttaat
     2341 atttggtggt ataacgatca caataccttc taagctctct tttagagagt tgcaagaaag
     2401 ggctctccat tcagaattat ctattgttaa tccaaagcaa gaagaagaaa ttaaaacgta
     2461 tattgataag ataaaaaaag aaggggatac tattggtggt attatcgaaa cgatagttca
     2521 aggggtgcca gcaggtcttg gatcttatgt ccaatgggat aaaaaattag atgccaagct
     2581 ggctcaagca gttctttcca ttaatgcttt taaaggggtg gaatttggag ttgggtttga
     2641 tatgggattc caaaagggct ctcaggtaat ggatgaaatc acctggacac ctacccaagg
     2701 ctatggtcgt caaacaaacc acttaggtgg ctttgaaggt ggcatgacca caggacaacc
     2761 tcttgttgtt aaaggggtta tgaagcccat tcctactttg tataaaccac tcatgtcggt
     2821 agatattgat agtcatgaac cttataaagc aacggtggaa cgctctgatc cgacggcctt
     2881 accagctgca ggggttatca tggaaaatgt ggtggcgacg gtgcttgcca aagagatttt
     2941 agagactttt tcttctacta ccatgtctga attgcaaaaa gctttttcgg actatcgtgc
     3001 ctacgtcaag caattttaga tagcgagata ctggctttta tggtatcata caggtattaa
     3061 tgaacaaaag gagactgtca tgtcacaaga aatttatgac tatgctaatc aacttgaaag
     3121 agcagttcgt gctctgccag aataccaaaa agtgttagaa gtaaaagagg ccattcaagc
     3181 agacgctagt gctagcgaac tgtttgatga gtttgtcgct atgcaagaaa aaatccaaga
     3241 gatgatgcaa tcaggccaaa tgccaacggc tgaagaacaa acaagcattc aagaacttag
     3301 ccaaaagatt gaagctaatg accagctaaa agcttatttt gaagcgcagc aggccttatc
     3361 tgtttacatg agtgatattg agcgtattgt atttgctccg ttgaaagatt tagtcaagta
     3421 agccaattaa gataggaaaa aaaacagatt gctggcagct cgtcaggatc tgttttttag
     3481 tgtttatttt ctttgtatca gaaagttgag cctctttctt atttgtgata tagtcaaata
     3541 aattgaccaa aatagcaggc cagtagtggc tttaatgaag gcaagttgtc tgctaactgg
     3601 ctatgctaaa acatcagtat tagaaagtaa aaggttataa aaatggttat tccttatgat
     3661 tatattgtta ttggtggagg gagtgcaggt attgcttctg cgaacagggc agccatgcat
     3721 ggggccaaag tattgttagc tgaaggtaaa gagattgggg gaacctgtgt taacctaggc
     3781 tgcgttccta aaaaagtcat gtggtatggc gctcaagtag cagatatttt agggacttac
     3841 gctaaggatt atggatttga ctttaaagac aaagcatttg attttaaaca attgaaagca
     3901 aatcgtcaag cttatattga tcggattcat gcttcttatg agcgaggctt tgagcagaat
     3961 ggtgttgacc gtatttatga ttatgctgtt ttcaaagatg ctcatacggt agaaattgca
     4021 gggcagcttt ataccgcacc gcatattttg attgccacag gaggacatcc tgtttttcca
     4081 gatattgagg gggcacaata cggtatcagc tcggacggct tttttgcctt agatgaggta
     4141 ccaaaacgta cggctgtggt tggggcaggc tatattgctg tggaacttgc tggtgttttg
     4201 catgctttgg gatcgaagac ggatttgttt atccgtcacg atcgaccttt aagaagtttt
     4261 gacaaagcca ttgtggatgt tttagttgag gaaatggcag ttggtggtcc ttgcttacac
     4321 cctcattcag aagtggcaaa ggttgtcaaa aatactgatg agtccttgac cctttacctc
     4381 aaagatggcc aagaagttga ggttgaccag ctcatttggg ctatcggtcg caagcctaat
     4441 ctagaaggtt ttggtcttga caaaactgga gtgacgttaa atgataaagg ttatattgag
     4501 acagatgcct atgaaaacac gtctgtcaaa gggatttatg cagtaggtga tgtcaatggt
     4561 aaactggctt taactccagt tgctgtggca gctggacgtc gcctatcaga gcgtcttttc
     4621 aatggcaaaa cagacgagaa attggactac caaaacgttg ctactgtgat ttttagtcac
     4681 cctgttattg gctctgttgg cttgtcagaa gaagcagcag tcaaacagta tgggcaagaa
     4741 gcagttaaaa catatcaatc acgttttacg tctatgttta ctgctatcac taaccatcgc
     4801 caaccttgtc tgatgaagtt ggtaacagtc ggagataccg aaaaaatagt tggtcttcac
     4861 ggaatcggat acggggttga cgaaatgatt cagggctttg cagtagccat caaaatggga
     4921 gcaaccaagg cggattttga taacaccgtt gctattcacc caactggctc agaagaattt
     4981 gtcaccatgc gctaaaaaat gtagcctaag gcaataaaaa atgatgttaa cagtttttga
     5041 ggaaagtctt ctaaactgct agcatcgttt tttgttaggt gattagggga atatgttttt
     5101 aaagatattt gcgttgctca gacaatattg ccttaccttg gagataaaat aaagtgaccc
     5161 tgtaacaaca aagagttcat cagatgttac ggaaccttgt aaccagtctc tgtaatcttt
     5221 tacctgtggg tatttatcag gataatcctc taaaagattt gcttcaggga aatcaaaagt
     5281 cgtgacagat acttcaaaag tatctagttg agccagcaag tcggctaaag gcttacgttt
     5341 gagacctgca aagaggatat gaataggacg tttgggaaat tcttcttgca ttagagactt
     5401 taatttagca atggaatctg gattgtgagc gccgtctaat agaagattag gttgaataaa
     5461 ttcggaccgg ccaggccaag ttgttgtctc aattcctttt tgaatcgttt ttggagatat
     5521 tttaggaaag gattttctaa gtaattgagc tgtcataatg gctaaacagg cattcgcttt
     5581 ttgatgttga cctaatagtt tgagacggag tttggttaaa ttcaagttat cgtgttgaaa
     5641 ggaaatcgtc ttttctgaag gattgagcaa aaaatctttt cctaattgat ataagcgtga
     5701 accggctttt tgggtaactt gttgaaagac ttgactagcc tcctgttcaa gttgaccgat
     5761 aataacgggt tctttagctt tgataacttc tgctttttgg tgagcaattt ctgctaggct
     5821 ataacctaaa cgttcttggt gatcaaaact aatggaaggg catacgacgg ccagagcatg
     5881 aaagacattg gttgcgtcgg ttttcccacc aatccctgcc tcaataatgg caatatcaac
     5941 aggtttaaga ttggcaaagt agcaaaacat tatgagagtc actaactcaa actcggtagg
     6001 tctatcccaa tttgtttcaa gggacatttt agtgagaatg ggtctaatca cgctcacaca
     6061 ttgcgccaat tctaggtctg aaatgggctg gccattaagg cagatccgat cgtgaaaaga
     6121 gatgataaaa ggagatgtaa aggttccgac ctgataacca gaagttgaca ggatatgctg
     6181 taagtaggct gtcacagagc ccttgccgtt agttccaaca acgtgaatag cagggaattt
     6241 gtcttgtggc cggcctagtg cctcaagtaa ccaagccatt ctggccaaat cggtcttgcg
     6301 accattagcc ttaaagctat gaatccagga aatgctatca taggtagtca ttactatttc
     6361 ttgtccttct aacaatgctt ctaagggttc gtaggaagac cagtattagc atcttcagct
     6421 ccagttgcat tagggagttg cgtcccttca gtggttgatg gtgtctcagg ggctgtagga
     6481 gttgtctcgt tgtgagaagg tgtggtagaa gatgacggaa gagttgtctt attatcatct
     6541 gtggtttctg gagtacctgt atggttttga ttaccctgag aagactcttt ttcaataatc
     6601 ttgacaatgg tattactcga ttctttgggt gtatcattag aggtcttatc tggtacaggc
     6661 tgcagtgctc ttgtaatggt aatggttgcc acaatgatac tcaaaatact accaataagt
     6721 gcaatggatt tttggaaagc tgtaaaacct gttttaatat gctcggtagg gacacctcct
     6781 gatttacgag gtttttgttg acgggaataa tgtgatgaca tatttttctc ctttaaaatc
     6841 atcgtaatgc caagattaag gaggaggaaa tagaagttct agctcctctt taacacttca
     6901 tctatcagta taatctagaa aaaaagtgaa gtcaacggtt taccttatgt ctaggacaag
     6961 gaagtccttt gggcttattg taatacgaat attcttgatt tcattttagc tattttgtga
     7021 tagaataaag tattgagtaa aaatgaaggt gacacatgat ttattttgat aatgcagcca
     7081 caaccatacc ttatggagag gctcttaaaa cttaccaaga agtagcaaca aaaatatatg
     7141 gcaatccctc aagtctgcac caactaggga caaatgcgtc tcgcatttta gaagcttccc
     7201 gtaaacagat tgctggtttg ttgggtgtta agtcagaaga aatctttttt acgtctggtg
     7261 ggactgaaag tgctaattgg gctattaagg gaattgcttt tgaaaaaacc gcttttggca
     7321 aacatattat tatctcagcc attgaacacc ctgccgtgag tgaaagtgtc aaatggcttc
     7381 tcacacaagg attcgaagtt tcttatgctc cagtgacaac acagggagtt gttgatgtga
     7441 atgctttagc tgagctgatt agaccagaca ccatcttaat ctcgattatg gctgttaata
     7501 atgaaatggg agctatccaa ccgattagag ccatttcaaa cctattggct aaccaaccaa
     7561 cgatcacctt tcacgttgat gctgttcaag ccattggtaa aataccactc tgtgattata
     7621 tgactaatcg tgtggatttg gcttcttttt cagggcacaa atttcatagt gttcgtggtg
     7681 ttggcttcct ttataaaaaa gcaggcaagc gactcaatcc tctcttgtca ggcggcggcc
     7741 aagagcagga gttacgttca accactgaaa atgttgcagg aattgctagc atggccaaag
     7801 cattaagaat agtcactgaa aaacaagtga gcgtcttacc taaactaaca gcgatgcgag
     7861 atgtgattta caaagctttg tccgcttacc cagatgtgac agtattttca gctcaagaag
     7921 gctttgcgcc caatattttg acctttggga ttagaggggt tcgaggagaa gttattgtcc
     7981 acgcctttga aaaatatgag atttatattt cgaccacaag tgcatgttcg tccaaagcag
     8041 gtaagccagc aggaagccta gtggctatgg gggttcctgt aaaaactgcc cagacagccg
     8101 ttcgtattag tctagacgat gacaacgata tgggacaagt agagcagttt ttgaccatat
     8161 tcaaacagat ttatgataaa acacagaaag ttaggtaaga ttaggtaaga tggactattc
     8221 agaaattatg gttcgccatg gtgaactatc aactaaaggg aagaatcgca tgcgattcat
     8281 taacaaactt aaaaacaata ttcaagacgt actggctcct ttcccagcca ttactgtccg
     8341 ttcagatcgt gataggaccc atgtgtctct aaatggcaca gattatcagc ctattgtaga
     8401 agctttaaag ctggtgtttg gtgttcaagc tctatctcct gtttataaac ttgaaaaaag
     8461 tgtccctctt ttggtaacag ctgttcaaga tatcatgact tctctttatc gtgatggact
     8521 gacgtttaag attgccacta agcgtagtga tcacgcgttt gaattggata gtcgtgagct
     8581 taacagcctt cttggtggag ctgtttttga ggttttaccc aatatccaag cccagatgaa
     8641 acatcccgat gtgactctca aagtcgaaat tcgtgatgaa gcagcttaca tttcttatga
     8701 agagattaag ggagcaggcg ggttaccagt aggaacgtca gggaaaggga tgctgatgtt
     8761 atcgggcggt attgattcac cagtagctgg gtatttagcc cttaaacgtg gccttgatat
     8821 tgaagttgtt cattttgcta gccctccgta caccagtcca ggtgccttgg ctaaagccca
     8881 agaccttact cgtcggttaa ctcgttttgg tggaaatatc caatttatcg aggttccttt
     8941 tacagaaatt caagaagaaa ttaaaaataa ggcaccagag gcttacctta tgacattaac
     9001 ccgccgcttt atgatgcgca tcacagatgc cattcgtgag caacgtaagg gtcttgttat
     9061 tgttaacggt gagagcctag gtcaggttgc tagtcaaacc cttgaaagca tgcaggccat
     9121 taatgcggta acctcaaccc caattattag accagtcgtg acaatggata aacttgaaat
     9181 tatcgagatg gctcaggcga ttgatacttt tgacatttct attcaaccgt tcgaagattg
     9241 ctgtactatt tttgcaccag accgtcctaa aaccaaccct aaactaagta atgctgaaaa
     9301 atatgaagaa cgctttgata ttgatggtct tgtccagcgt gctgtgtcag gaattgttgt
     9361 cacagaaatc acacctgaaa tcgtcaatga tgaggttgaa aatctcattg atgctttact
     9421 ttaaaagaaa agatcaagtg taaatctttt agtagtcagt aatgatacta ttgaaaagcc
     9481 tctataaggg cttttttgct gcaatgaagg aggaaaattt tgaggcaaga tgttataata
     9541 gagaaaaagg agggtgtgtc ttgattaaaa ctatgcaata caaaaaaacg atggcaacag
     9601 ttgttgctct tatcgtcgca cttttgctat ttggcttgat ttatgattta cttggggttc
     9661 aaaaaaatga gctagcagct caaaaatctg ctcagccaaa ggtcaaagca gcgcgtgttg
     9721 tagctaatgg cgacatttta attcatgata ttctctacat gagtgctaga aaagctgacg
     9781 atacctatga tttcactcct tattttgaat atgttaagga ctggattagc ggtgctgact
     9841 tagctattgg agattatgaa gggaccatta gcccagatta tcccttagca ggctatccct
     9901 tattcaatgc tccagaagag attgcaggtg ctctgaaaaa tactggctat gatgtagtcg
     9961 atttagccca taatcatatc ttagattccc aattagacgg tgcccttaac actaaaaagg
    10021 tttttcatca attaggtata gacagtattg gcatctatga caaggatcgt tcaaaagaat
    10081 ccttcttaat taaaaatgtc aatggtatca aaattgcaat tttaggttat tcttatggtt
    10141 ataacggcat ggaggccaca cttagccaag aagactatga gaaacacatg tctgatttag
    10201 atgaagctaa gataaaaaaa gaacttcagc tagctgaaaa aaaggctgac gtgaccattg
    10261 tcatgccaca aatgggaaca gaatatgcct tagaaccgac agcagagcaa aaagaacttt
    10321 accacaaaat gattgattgg ggagctgacg tcgtcctagg aggccatccg catgttattg
    10381 aaccttcaga gacagttata aaaggtaggc aaaagaaatt cattatttac tctatgggaa
    10441 attttatttc aaatcagcgg cttgaaaccg tagatgatat ctggactgag cgtggcctat
    10501 tgatggacct tacttttgag aaaaaagata acaaaacaaa aattaaaaca gttgaggccc
    10561 atccaaccat ggttttagcc aaaggtaaag gtattgtggg taaagaaggt tttgaactat
    10621 acaattatcg aacaatggtt ttagaagatt ttatcaaagg tggaaaatac cacgacaaga
    10681 ttgatgagga aaccaaagca aaagtagcac ttgcttatca ggaaattaat gatttagtta
    10741 acctgaaatg gtaatgtttg gtggtttagc atctaatggc caaatttcaa gttgaagtta
    10801 gccagtttta acatttctac tggcgacttg tagttgaaga gtcgtttagg ataatgattg
    10861 atccaatgtt caatagcagc gacttcttta ggggtcgttg ttttggtacc tttagagagc
    10921 catctgagaa taaggcgatt atggttttcg ttagtgcctc tctcccatga cgcataggga
    10981 tgggcgtagt agatggcact tgctggaacg acatctgata agcaagaaaa ttctgtcccg
    11041 ttgtctgctg tgatagagtt gacggtgtag tcttttagaa tccccgtaag agcttcattg
    11101 acggcttgag cagacttatc cggtattaat cgaatcagct gatgacgtgt tttcctgtcc
    11161 gttagggtga gtaagcattg atttttagcc ctcgtcagaa tgactgtatc aatctcataa
    11221 tgcccagctt caagacgttg gttaatgcta tctggacgtt gttcaatact ctttcctgcg
    11281 ggtttaaaat aaggactggc ttgttttcta gcctgcttag cctttctcgg ataaagcata
    11341 gcctccttac caaggcttaa tttgccatga tgaatccagt aatagatggt agatacaggg
    11401 acttgaactc ccttagcttt aaccatcatc tcaggggaat aattctgatg catgtagtgt
    11461 tcaatagcct ccctatcttg cttacgctgc ctaatgggtc taacagaacg cttgcgattt
    11521 tcttgataga tcaactgagt atgttgagca gaataaacgg cttcaaactt tcctttacga
    11581 acctgttgcc taagcaagcc tcgcttgatt tcattatgat tggtctgggg tgccttacct
    11641 agtaagcgag caatttcacc ccgttctgtg gctgaaagat gagaatagga ggattttctg
    11701 gtagaattaa tattagacat gttcatctgc tttccatact gaattgagga attctagtat
    11761 atcagacgaa catgtctttt ttgttgcact tcattttaca acgcgggaat gataaaaaat
    11821 agagattaaa cttaattttt ccttgataaa aagcagttat ggtgctaaaa tagtaacgtt
    11881 gactaaatgc acatcctgtg caaccgcgcg aaaatcgtta tcaagtagac caagtctcac
    11941 gattccaggc gagtcttcac agagggaaac ctcaaacaaa aattttatag gaggtgcatc
    12001 atgagcacat acgcaatcat caaaactggt ggaaaacaag ttaaagttga agtaggtcaa
    12061 gcaatctacg ttgagaaaat tgacgctgaa gctggcgcag aagtcacttt taacgaagtt
    12121 gttcttgtag gtggtgacaa aactgtagtt ggtactccag ttgttgaagg agctactgtc
    12181 gttggaactg ttgaaaaaca aggaaaacaa aagaaagttg taacattcaa atacaaacct
    12241 aaaaaaggta gccaccgtaa acaaggtcat cgtcaacctt acactaaagt tgtcatcaac
    12301 gcaatcaacg cttaatttac tagcttatga ttaaagcaat ttttactcgc caaaaaaatg
    12361 gtcaactgag cagtgttaca ctgacaggtc atgctggtag tggtaaacat ggctttgata
    12421 ttgtttgtgc ctctgttagt actctagcca ttaactttgt taattctctt gaggtgttag
    12481 cagattgcca agcattagta gatcttaatg atgttgaagg tgggtatatg gctattacaa
    12541 taccaccaca tgacaacaag gaggaagttc aattattatt tgaatctttt cttctaggaa
    12601 tgactagttt ggcgaaggat tcttcgaaat ttgtcaacac tcaggtcatc taaatttaat
    12661 ttaagagagg aaacaacatt atgttaaaaa tgaatcttgc taacttgcaa cttttcgccc
    12721 acaaaaaagg tggaggttct acatcaaacg gacgtgactc acaagctaaa cgccttggtg
    12781 ctaaagctgc tgacggtcaa actgtttcag gtggatcaat cctttaccgt caacgtggaa
    12841 ctcatatcta cccaggtgta aacgtaggcc gtggtggaga tgacacactt tttgctaaag
    12901 ttgaaggtgt tgtacgtttc gaacgtaaag gacgtgataa aaaacaagtt tctgtttacc
    12961 cagtagctaa ataattgttt aacatcgcat tatataaggc tttccaggtg tctggaaagc
    13021 ctattttttt attttggtac tcatgttgct acccatattt taaaaatcaa tgtaggaaga
    13081 gagcttgtca gcagttttat cttttgagga tttagtaaca tgagcgtaga tgttcatggt
    13141 agtttggata gtttcatgac caagtgtatt ggattttcat tgtttgtttc tatcctacta
    13201 aagattgtag ttgcttattt agagcaatag ttctaattgt aaaaagtcag cataataaaa
    13261 caatgtcata agatgttgtt ataacgggct ttatggtata attaaggata ctcattttag
    13321 gaaggaaagc aatgaatatt caacaattac gctatgtggt agccatcgct aataatggaa
    13381 cttttcgaga agctgcctct aaattgtttg tcagtcagcc tagcttatca gtttctatca
    13441 aagatttaga ggcggaatta gggtttcaaa tttttaatcg gactacttca ggaacagtct
    13501 tgacaagtca aggactagtt ttttatgaga aagctttaga agtagtcaaa tcttttgatt
    13561 cttttgaaaa aaccttttct caagctgatc tggatcaaaa tgaattttct attgctagcc
    13621 aacattatga ctttttgcca cctcttatta cagctttttc gcaacaatac gatggtcatc
    13681 gtgtctttcg tatctttgaa tcaacaacta ttcaaatttt agatgaggtt gctcaaggaa
    13741 acagtgaaat tggtattatt tacttgaatg tagacaatca aaaaggcctc tttcagcgga
    13801 tggataagct gggcttggaa tatgtttctc taattccttt tactactcat atttatcttt
    13861 caaaaacgca tccattggct aatcgcgaag cgctttattt gagtgatatt caaggattac
    13921 ctgcggtaag gtttacccaa gaaggggacg agtatttgta ttattctgaa aattttgtgg
    13981 atactagtga atgccctcgt atttataatg tgtcagatag ggcaaccttg aacggtattt
    14041 tagagagaac caatgccttt gcgacaggat caggttttct cgatcatcgg agtgttaatg
    14101 ggattaaagt gatacctcta gctgatcata ttgacaatca aatgatttat gtcaaacgaa
    14161 aagataaaaa cctttctgtt gctggggcca cttttgtgac tattttaaaa gactattttg
    14221 ataaaagaag gaaggcctta acatgaaaaa acgattgttt gtgcttagct tgatcctcct
    14281 tgtagctttg gatcaactta gtaaattttg gattgtttct catatagcgc ttggagaagt
    14341 gaaacccttt atcccaggta tcgtcagctt gacttacttg caaaacaatg gggctgcctt
    14401 ttccatattg caggaccagc aatggttctt tgttgtcata acggttttag ttatcggtta
    14461 tgctatttat taccttgcta ctcatcccca tttaaatatc tggaaacaat tagctctctt
    14521 gcttattatt tctggtggaa tcgggaattt tattgatcgt ttgcgtttag cttacgtgat
    14581 tgatatggtt catttagact ttgtggattt tgccattttt aatgtggcag attcatacct
    14641 taccgttggt gtcatattat tagtgatatg tttatggaaa gaagaggatt atggaaatta
    14701 atgtgataac atcggggcaa cgcttggata aggcactagc tgatttaagt cccctgtcac
    14761 gtggtcaagc caatgaccaa attaagcaag gccttgtttt agttaatggt cagcaaaaaa
    14821 aggctaaata tacggttcaa gcaggagatg tgatttgttt tgaactccct aaagaagaag
    14881 tcttagagta ccaagctcag aatattccct tggacattat ctatgaagat gatactctcg
    14941 ccattattaa caagccacag ggaatggtag ttcacccttc ggcgggacac ccttcaggaa
    15001 caatggttaa tgccttgatg tatcatatca aggatttgtc ctcaatcaat ggtgtggttc
    15061 gtccaggaat tgttcatcgg attgataagg acacctcagg tctcttaatg gtagctaaaa
    15121 cagatgcagc tcatcaagcc ttggcagaag aattaaaggc taaaaaatct ctcagaaaat
    15181 acctcgccat tgtccatgga aacctaccga atgatcgcgg gatgattgaa gctcctatag
    15241 gtcgtagcga aaaggaccgt aaaaaacagg ctgttacagc aaaaggcaaa gaagcagtga
    15301 cacgttttac agtgctagaa cgttttggcg attacagtct agtggaatta caactggaaa
    15361 ctggtcgaac gcaccaaatt cgtgttcata tggcctatat tggtcatcct gttgctgggg
    15421 accctttata tggaccacgt aagacactat caggtcatgg ccaatttctt catgcaaaaa
    15481 cattgggatt aacgcatcct atgacaggaa aagaaatgat atttactgtt gaagctcccg
    15541 aaatttttca aaaggtgtta aagctgctaa gaaaaaaata aacagcgagg agtagccatt
    15601 atttttagtc ttatatcttt gtgtaaagtt tttaacatta taaaaaatac taaaaagcat
    15661 ttacgataag ccaggaaata tgagaagtct tttatgttat tgggatatgc aacatatctg
    15721 ttagagatct tt
//
LOCUS       DAUELZ010000039        15645 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.039, whole genome shotgun sequence.
ACCESSION   DAUELZ010000039 DAUELZ010000000
VERSION     DAUELZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15645
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.039"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            215..898
                     /locus_tag="VHI09_000926"
     CDS             215..898
                     /locus_tag="VHI09_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEQ6120810.1"
                     /translation="MEWIKLIGIVIIVLGFILKFDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQALTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            898..1824
                     /locus_tag="VHI09_000927"
     CDS             898..1824
                     /locus_tag="VHI09_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HEQ6120811.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPVFNERKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            1874..2521
                     /gene="pcp"
                     /locus_tag="VHI09_000928"
     CDS             1874..2521
                     /gene="pcp"
                     /locus_tag="VHI09_000928"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEQ6120812.1"
                     /translation="MKILITGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(2636..3346)
                     /locus_tag="VHI09_000929"
     CDS             complement(2636..3346)
                     /locus_tag="VHI09_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEQ6120813.1"
                     /translation="MITKGQVRLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGFSKV
                     GVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKTTCNCLGAYPF
                     EGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELEYVD
                     GYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHLDQQ
                     RFLITGKLYPLMLEVVLD"
     gene            complement(3361..3828)
                     /gene="smpB"
                     /locus_tag="VHI09_000930"
     CDS             complement(3361..3828)
                     /gene="smpB"
                     /locus_tag="VHI09_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEQ6120814.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGTG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHDYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            complement(3831..6164)
                     /gene="rnr"
                     /locus_tag="VHI09_000931"
     CDS             complement(3831..6164)
                     /gene="rnr"
                     /locus_tag="VHI09_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEQ6120815.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSALDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINSQGHVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVTLHRILEAMRSKRGALNFDTQEAKIIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKVEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYNQPSQEKRDHFAQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGTK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            complement(6258..6494)
                     /gene="secG"
                     /locus_tag="VHI09_000932"
     CDS             complement(6258..6494)
                     /gene="secG"
                     /locus_tag="VHI09_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEQ6120816.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            complement(6540..6686)
                     /gene="rpmG"
                     /locus_tag="VHI09_000933"
     CDS             complement(6540..6686)
                     /gene="rpmG"
                     /locus_tag="VHI09_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ6120817.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            complement(6683..7876)
                     /locus_tag="VHI09_000934"
     CDS             complement(6683..7876)
                     /locus_tag="VHI09_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEQ6120818.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVAPILSLYIRHLGQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVFAYNQMSSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            complement(7998..9500)
                     /locus_tag="VHI09_000935"
     CDS             complement(7998..9500)
                     /locus_tag="VHI09_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEQ6120819.1"
                     /translation="MTTMTIAKGNQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISEMGPMMLSRLLNLNDTQTGILNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNIAKQSIGAIQRSLLTLEQEGAHQFFGEPALDVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVTQSPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSEI
                     ESQQLVQSSPFASKYSQSIDRESAYEKLAAKVLEDNRLAQEAIATAQREKEAKEAIKA
                     QAATKKANRRSVGRPRKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            complement(9690..10283)
                     /locus_tag="VHI09_000936"
     CDS             complement(9690..10283)
                     /locus_tag="VHI09_000936"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEQ6120820.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPLLMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNNGDMETLIKQVQSALLSLANPR"
     gene            complement(10280..11107)
                     /gene="mutM"
                     /locus_tag="VHI09_000937"
     CDS             complement(10280..11107)
                     /gene="mutM"
                     /locus_tag="VHI09_000937"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEQ6120821.1"
                     /translation="MPELPEVETVRRGLEALVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKETFKLKTFETALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPETASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRP"
     gene            complement(11279..12145)
                     /gene="rgg2"
                     /locus_tag="VHI09_000938"
     CDS             complement(11279..12145)
                     /gene="rgg2"
                     /locus_tag="VHI09_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEQ6120822.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQKEISGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSGADL"
     gene            12234..12302
                     /gene="shp2"
                     /locus_tag="VHI09_000939"
     CDS             12234..12302
                     /gene="shp2"
                     /locus_tag="VHI09_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEQ6120823.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            13216..13485
                     /gene="stcA"
                     /locus_tag="VHI09_000940"
     CDS             13216..13485
                     /gene="stcA"
                     /locus_tag="VHI09_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEQ6120824.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHS
                     KQNPHKKEALKHKKNKNKTLFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(13833..14231)
                     /locus_tag="VHI09_000941"
     CDS             complement(13833..14231)
                     /locus_tag="VHI09_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEQ6120825.1"
                     /translation="MHRLLNRLIPEEDNNKLSRAAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLTHHLLHSDGILPSLNDKSA"
     gene            complement(14324..15394)
                     /locus_tag="VHI09_000942"
     CDS             complement(14324..15394)
                     /locus_tag="VHI09_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120826.1"
                     /translation="MMMLRDVKVKMSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGV
                     GYKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQTV
                     PGMGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMYNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGAFPDNIK
                     VSINPKGRQATITYGDGSTDIIPPAVLWKKGSVKEPTETDQSVGTPTPGIPGKFKRDQ
                     SLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVG
                     SWLEPATALPSVEMSAEDRLKS"
BASE COUNT         4586 a         3448 c         2787 g         4824 t
ORIGIN      
        1 ttaaattaaa acatttttct catcaatcgt ttcctactcc ttctgtgcgc gaagctttct
       61 ttttaagccc gttataccaa atgataatga cctttccaaa atagtatcac cttccttaac
      121 actttaagta gaaaaggaac tattaggttg agcctttttg caaatcctag cgactaatgt
      181 tataatcgtc atactattga aaaaggagtt ctttatggag tggattaaat taatcggtat
      241 tgtgattatc gtattagggt ttattttaaa atttgatgct atcgctaccg tagttgttgc
      301 aggacttgtt accgcgctgg tttcaggaat ttcgtttatt gattttctag atattttagg
      361 aaaagaattt accaatcaac gtttgctaac cattttcttc atcactttac cgcttatcgg
      421 cttgtcagaa acttatggac tgaaacaccg agctactcag ttgattcaac gtgttcaagc
      481 ccttaccgtc ggccgtttat tgactctcta ccttattatt cgagaattag caggtctctt
      541 ttcgattcga ctagggggac atccacagtt cgttcgcccc ttgattcaac ctatgggaga
      601 agctgctgct aaagccaaca ttggagaaga gttgaccgat gcagaaaaag atgatatcaa
      661 agctatggca gctgccaatg aaaactttgg caatttcttt gcccaaaata cctttgttgg
      721 cgcaggaggt gtcctactta tcgctggaac tttagaacag cttggctatg atggtaacca
      781 agctaaaatt gcattttctt ctattttaat tgctattatc tctattatta tagttgctat
      841 ttacaattac ctctttgaga aaaaaatgga acgtcaacat cagaaaggag ataactaatg
      901 gctaacattg caaaccttgt tttagaaacg atatatatcc ttatcggctt gcaactgttt
      961 cacacagcct actgcacctt taaagataaa accaaccctg tctattttgg gacagctctg
     1021 ttctggggat tgctgggtgt taccttcgtt ggcggtgcct ttttgcctaa caaagttatc
     1081 ggttttatcg tgattgtcct agctctttta accttgttta agcaagttcg tattggaaca
     1141 ttaccagtct ttaatgaacg aaaagctgaa gaaagtgctc accgcattgg taactggatt
     1201 ttcttaccag ttatgttaat ggctatgatt tctctacttc ttgccttgat ccttcctgat
     1261 ttcagtaaaa gtgccattgg gattgctggt atcttagcca ctattgctat tttaataatc
     1321 actaagcaaa aaccatctgc tcttttagct gaaaataatc ggatgaacca acaagtgtcc
     1381 acaagcggta ttttgccaca gctcttagga gctttaggag ctatttttgc agctgctggt
     1441 gttggtgatg tgattgcttc tctcattcgg gagattgtac ctgctgatag ccgcttcttt
     1501 ggtgttctcg cttatgtgct tggcatggtt atttttacta tgattatggg aaacgctttt
     1561 gctgccttta ccgttattac aaccggtatc ggtgttcctt ttgtttttgc tttaggagct
     1621 gaccctatta ttgccggtgc tttagctatg actgctggtt tttgtggtac cttattaaca
     1681 ccaatggcag caaactttaa tgccttgcct gttgctttga tggagatcaa agatcgaaat
     1741 gcagttatca aaaagcaagc tcctatcgcc cttgttttaa ttattagtca cattgcctta
     1801 atgtatttac ttgcttatta ttaacttttt tgctaagtta gtagcgtcag ttattcattg
     1861 aaaggacatt attatgaaaa ttcttataac aggctttgat ccctttggcg gcgaagctat
     1921 taatcctgcc cttgaagcta tcaagaaatt gccagcaact attcatggag cagaaatcaa
     1981 atgtattgaa gttccaacgg tttttcaaaa atctgccgat gtgctccagc agcatatcga
     2041 aagctttcaa cctgatgcag tcctttgtat tgggcaagct ggtggccgga ctggactaac
     2101 gccagaacgc gttgccatta atcaagacga tgctcgcatt cctgataacg aagggaatca
     2161 gcctattgat acacctattc gtgcagatgg taaagcagct tatttttcaa ccttgccaat
     2221 caaagcgatg gttgctgcca ttcatcaggc tgggcttcct gcttctgttt ctaatacagc
     2281 tggtaccttt gtttgcaatc atttgatgta tcaagccctt tacttagtgg ataaatattg
     2341 tccaaatgcc aaagctgggt ttatgcatat tccctttatg atggaacagg ttgttgataa
     2401 acctaataca gctgccatga acctcgatga tattacaaga ggaattgagg ctgctatttt
     2461 tgccattgtc gatttcaaag atcgttccga tttaaaacgt gtagggggcg ctactcactg
     2521 actgtgacgc tactaaacct attaaaaaaa cagagatatg aactaactct gttttttttg
     2581 tgctaaaaat gaaagaccta gggaaacttt tcataggtct ttctcaattg tcatcttaat
     2641 ctaatactac ttctaacatc agcgggtata gtttaccagt aattaagaaa cgttgttgat
     2701 ctaaatgagc aatcccattc aaaacattaa ggtcagggta atgggactta tcaagattta
     2761 aggcttttaa caaaggacta atatcatagg tggctaccac ctttccagaa tcaggttgga
     2821 gtttgacaat agtattggtt tgccaaatat tggcatagag ataaccatct acatactcta
     2881 attcgttaag cattgagatg gggacacttt ctatagcaac tagtacggta tccaataaag
     2941 caaaatcttt ggggtctcgc ttttgcaaaa aagcatttcc actagtcatc caaagacact
     3001 ggttttcctt atcataggct aatccccagc catctccttc aaatggatag gcccctaaac
     3061 aattgcaggt agtcttatca aacttgtagg ctacaccctc tttataggtt agtaaccaaa
     3121 aataatcctc aacaactgtt agcccttctg caaaaactgt atctggaaaa gctatttttt
     3181 ctgaaaagat ctcttgagta aggtcgtaca cccctacttt tgagaaacca taacgaccgg
     3241 cactaagtaa tatatgattg ttgttgagtt gttctaatcc ctgcgtgtat aagttgctat
     3301 cataagaata agttcgtaac aaacgaacct gtcctttagt tatcataata ctcgttcacc
     3361 ttaccttgca ttgtagtgtt tcatttgttt tttaatatct ctttcctgat cacggcgttt
     3421 aatggtttcg cgtttgtcat aatcgtgttt ccctttagct agaccaatca gaactttagc
     3481 aaagccatcc ttgaggtaaa ctttgagagg aaccaaagtc atgccagttc ctttgagttc
     3541 atttgctaga tgcgtaatct cacgtttttt gagcagcagt ttgcgggttc gttcaggatc
     3601 agcgttccaa atattacctt gttcaaaagg tgcaatatgc acgttgacca accacgcttc
     3661 cccattctta atttgggcaa agccatcttt cagttgaata cgagctgcac gaacactttt
     3721 aatctcagtt cccgttaaga caatccctgc ttcaacggtt tcaacaatgt gataatcatg
     3781 tctagccttt ttattttgcg ccagtatatg accttcacct tttgccatag ttaactcctt
     3841 ttacgactct tcttttttgc ttgttctttg taaaatggtt ttttagaccc ttttttagtt
     3901 ttacccttgg tttttgcctt ggctacttct ttaggttctt ttttctttgt ccctttagat
     3961 gattttgagg atttacggcg atctcgtcga ctagctttat ccgacatctt gattttttca
     4021 accacatcaa agtcgctagg taaatactca aagtcaatgt caccagtttc cttgtcagcc
     4081 ttaaccaatt tcacacgaat aggttgaccg accttaaaga ccttacctga tttttcacct
     4141 tgaaggctta gcgttctttc attaaagtga tagtattccg gtaagctagt gatatgaacc
     4201 aaaccttcaa tcgtatttgg caattctaca aaaaaaccaa atttaacgac actggccacg
     4261 ataccgtcaa attcttcacc cacatactct gccatatact cagctttttt catggcttcc
     4321 accacgcgtt cagcatcaat agcacgacgc tctaactgtg aagaagatgt tgccagctct
     4381 ggaatgatct gagcaaaatg gtcgcgcttt tcttgactag gttgattgta ctcacgaacc
     4441 atgcggtgaa ccaataagtc agggtaacga cgaatcggac tggtaaagtg agtgtaatat
     4501 tctgcggcca aaccgtaatg gccatgatta tgttctgaat atcttgcctg ttgcatagag
     4561 cgtagaagca tcatgttgag cacttctgct cctggttgac cttcgacttt tgccataaag
     4621 gcttgcaagg cttcctgaga aatcttgttg gctgttcctt gaatgtgaat accaaaggtg
     4681 ctagcgtaat caataaattg ttgaagtttt tcaacctttg gttcttcgtg aatacggtag
     4741 ataaacggta acttagcttt tgcaaaatgt tctgccacac actcattggc tgctagcata
     4801 aaggattcaa tcatccgctc tgcaatgccc ctttgacgaa gcaccacatc aaccggcatt
     4861 cctttgtcat tgacaataat ttttgcttct tgcgtatcaa aattcaaggc gcctcgtttt
     4921 gaccgcatag cttccaagat cctatgtaag gttaccataa gggtcacatc atcagcaatg
     4981 ctagcgaatt cttggagcgc ttcttcatct cctgcaatca tgtcattgac cgtactatac
     5041 gtcattcggt aagttgtctt aatcactgat tgacagattt gataattgac cacatgccct
     5101 tgagaattaa tttccatcaa ggctgactgg gtcaaacgat caacgttagg gttcaaagag
     5161 cagatgccat ttgacaaacg ctctggtaac atcggaacca cacgatcggt gacatagaca
     5221 gaagtgccac gggcgatagc ttccttatca agggcagatc cttctgtcac ataatatgag
     5281 acatccgcaa tatgtacccc aagttcataa ttcccgttat ccaatagctt aatatggata
     5341 gcatcatcta agtctttggc gtccgcacca tcaatagtaa tggtggtttc ttgacgcaaa
     5401 tccacacggc ctattaaatc tttagcagtt ggtgcttctg aaatagcatt ggcctcagct
     5461 aaaacttctg ctggaaattc tgacacgata tccatcgatt caaggacttc taggacatca
     5521 attccaacgt ctccttgatg gcctacgata tcacgcacac tggctacaaa ataatcgtgc
     5581 ccgcgtgtgg ggtatttatc aatatcaacc ttgatgattt cagtgccttt aagcacaaca
     5641 ggttcttttt tgatatagat tttttgttga atcttttggt ttttggacct gatataacca
     5701 gcatattttg gtttgtcatc atctaaaata aaggttccaa cagctgtttt taaagaacgg
     5761 tcaacaatag caacgacctt agcttcagcc gctgtgcctt tgagacggtc tgctggcttt
     5821 ttaaccacta cttcgacagt atcaccatca atagcatacc caacatcatt acgaccgata
     5881 aacatatcgt cttcattctc atccacatgt aagaaaccaa agcctgcttt attggcacga
     5941 aaaacaccct gaacagtagg ttcttttttc ttttcacgct cttttctaag agccagactg
     6001 ccatcatctg aaaaccgtaa aaggctctga ctttccattt tggaaatggt tttaataagg
     6061 ctgggaaaat gcttagctcc agccatctcc aaggccgcag ccaagtcgtt cacattggac
     6121 ttgccatgct ccttgaggta agtcataatt ctttctttca tcatacctaa tttctatttc
     6181 tgttttgttt gttggaggat atcaatccgt ttcgctgata aaaaaatgag ctagactaac
     6241 tctgtcaagc tcactcctta tttacttgat aaaatggcaa gcacaagtgc aatcgctagc
     6301 caaaaaaata ctagaactgc tgtgaatcgt tgcatgaatg cttcaaagcc acgagctttt
     6361 gtacgttcaa acaaggcttc tgaaccgctg ctatcaaaaa cattgctact tgggtttttt
     6421 tgaggttgca taaaaatagc aatcacaagc aataccgaca gtactagcaa tatcgtaagt
     6481 agtaagttgt acatgttatt tcctccacta ttgtcatgac cattctaaca caattcttat
     6541 caagtttcaa catgcagggt cacttttctc tccttggggc agtatttggg aacttttatt
     6601 ttttccggat gactgctttt atttttactg gttagataat tgttactgcc acactcacta
     6661 cattctaaat taatttttac tctcacacaa tatccttaac tttaatatat tttcgaaata
     6721 taatcaaact ccatattaaa ttaacaaaca caatcaaact agtcacataa aaaacagaac
     6781 ggtagcctaa cactacagcc acatttgatc cgataaaagg gccaatgact tgaccaaggt
     6841 tactagacat ttgattatag gcaaatactc tagaaatacc ttcttttggt gttaacttag
     6901 ttagtaaaga gttaatactt ggcatcaaag cgcccacacc aaacccataa gcaaagcgta
     6961 gtactcctaa ttgaaaactg gtctgcgcta aggcactgct aaaatacatg ataaagctat
     7021 aacacaaagc ggctaacaat aaccgatgat tgccaaaacg atctcctaat tttcctaagt
     7081 aagaactgct aaataagcta gaaaacccca tggccgaaac taccaggcct gaagtaaaca
     7141 ttaagttgtg agtttgacca agatgacgaa tatacaatga taaaatgggg gccactgatt
     7201 gagctgaaat ttgaataatc atactagtca caaacaaacc aagcataatc tggggacttt
     7261 ttacctgttt taaaatgact tttgtaggta tcatctcaaa gcgcctaatc ggcttaaact
     7321 cttctttaac ataaacagct gtcattaaag aacataaaaa taatatcaca ccaactaata
     7381 agaaaacttg tcgaattcct aatagctctg ccaaaatccc ccctagtaaa ggaccaatta
     7441 aagcgcctgc tgtcactcct gttgctaatg tgcctaaagc ataacctgac tcctcttttg
     7501 gggcttggct ggcaatcaag gcagtggcat ttggcacata gcccgcaaag acacctgtta
     7561 ataatctcaa aatgagtaac caaaacacat taggaataat agctaaacca cccatggtaa
     7621 aggtcatgac aaaactggct cgcagcatca taggttttcg gccataacga tcagccaact
     7681 tcccccaaac aggagcaaat aaagctgaag caagtgccgt cacagcaacc gccagaccag
     7741 catagtattc tactaattct gtcggagtac ctaaattttc cacatataga gccataaaag
     7801 gcataacaag tgaaaagctt gccccagtaa agaagttacc aagccaagcc acttttagat
     7861 tttgtcgcca gttcactgtc gttaccgctt tatcactaat agatcatcat cttctttcca
     7921 ttgggccttt gattattata acacatttct gccaaaaaca gtgtcccata atagtgctaa
     7981 gtctcatctg atagccttca tcgtttacgt aaactaccca gtaaacctct gaccagttct
     8041 cgcccaattg ttctaaccgt cgtactaata aacgcatctg ttgctttttc gacgactgtt
     8101 tttcgtggac gcccaacaga acgccgatta gcttttttgg tagctgcttg cgccttgatg
     8161 gcttctttag cttctttttc gcgttgtgct gtcgctatcg cttcttgggc cagtcggttg
     8221 tcttctaaaa ccttagcagc taatttttca taagcagatt cacggtcaat ggactgacta
     8281 tatttactgg caaaaggtga ggactgcaca agctgctgac tctctatctc tgacaaaaca
     8341 gcaaagctac tcttaggcgg catgatatag gcacgttcaa cgatggatgg ttgtccctta
     8401 tcattcaaaa cagaaatcaa ggcctcaccg acttctagtt ctgtaatcac ccttgccaca
     8461 tctaagtcag gattttgtcg gaaagtatcc gcagcgacac gaaccgcctt ttgttctttc
     8521 ggtgtataag cgcgcaaggc atgctgaata cgatttccaa gctgagccaa aaccgtttct
     8581 ggcaaatcaa gtgggctttg agtcacaaag aaaatcccaa ctccctttga acgaataagt
     8641 cgcacaatct gttcaacttt ttccaagaaa actttagggg catctttgaa gagcaagtga
     8701 gcttcatcaa aaaagaaaac catcttaggt ttgtccaaat ccccaacttc tggcaataat
     8761 tcgtacaact cagacaagag ccaaagtaag aaggttgtgt aaagggtagg agattgaaaa
     8821 agcttagtcg ctgacaaaat gttaatggct ccatagcccg aagcaacatc taattgcatc
     8881 aaatcagcca catctaaagc aggctctcca aaaaattggt gggctccttc ttgttctaat
     8941 gtcaagagac ttctttggat agccccgata gattgcttgg cgatattacc gtaatggctg
     9001 gagtagtctg aggcatggtc accaacttct tttaagatag cctgcaagtc tttcaaatca
     9061 atcaaaagcc agcctttctc gtcggcgatt ttaaagacga tgtttaagat acctgtctgg
     9121 gtatcattaa gattcaataa acgcgacaac atcatcggtc ccatttcaga aatcgttgtc
     9181 cgtaagggtt gaccgttttg accaaaaaca tcccacattc ttacaggaaa ggcctgaggc
     9241 tggtaatcac ttaccccgat tgtggctaga cgcgcagcca atttatcagt cacctctcct
     9301 gctttagtaa gattagacag gtctcctttg atatctgcca aaaagactgg cacaccagct
     9361 aaacttaatt gctctgctag cacttttaag gtcacagttt tacctgttcc agtcgctcca
     9421 gcaatcaaac cgtgacgatt tagcatccct aattgtaagg ctatcggttg attgcctttt
     9481 gcaattgtca ttgtagtcat aggcttcctc ttttttgatg atcatacttg tcgatatgtg
     9541 tttacccata agttaaagtg tttagaaggg tctcgctctt aatactatta taacatataa
     9601 cccttgatgc gctaatttaa aagggaccag ctagttttag acagaaatag cttgattttg
     9661 acagctatga tacgaatctc tcagcttagc tacctcggat tggctaatga caacaacgca
     9721 gattgtactt gctttattaa cgtttccatg tctccattat tatcaatcac tagactggca
     9781 tagggctttt tttcctctat tggtaactga ctggctattc gctgccttgc ttttcctttg
     9841 tctaaacgat ttctagccat taagcgctgc aattgtgtct gagcatctac ataaaccagc
     9901 caaatcgcat caaaccagtc ttggtagccc aattccatta aaaggggaat atccatgaaa
     9961 aaaatggctt ggctctgtgc taagtggtca cgcttagccg ccaattcttc cttaatgatt
    10021 tgattttgaa tggctgagga tgttgccata ttatctgggt ttgaaaaaag catttctgac
    10081 agctttgtgc gatctaactc accatctgct tttaaaatct gattcccaaa agcttctcta
    10141 agagcctcgt aaaggcgacc gcctttttct tgcaagtcat ggaccacttg atccgcatca
    10201 atcacctgat aaccagcttt tctaatgacc ttaaccacag tcgattttcc tgatgcaatg
    10261 cctcctgtga tgccaataat catggtcttt ttttctggca ttttggacaa atgtgcgtcc
    10321 cccttcctcc aacttttaat ttgacaatag cctgtccaca acgaggacac ggtttcccag
    10381 tttgaccata aacctgcaaa tagtcctgca tggtgccatc agctcccaaa gcattacggt
    10441 aagtacggac ggtcgaaccg cccttttcaa ttcctaaagc taggattcgt atggtttcat
    10501 catgcagccg ttttatctcc gccttattca acctagaaga cgcagtttct ggatgaacct
    10561 tagcagccca aagtacctcg tccacataaa tattccctaa accagcaacc aaggtctggt
    10621 ctaacagatg tggttttatg ggtttttggg aggatagcag agcagtctca aaggttttta
    10681 acttaaaggt ctctttcttt ggttcagggc ctagttttcg tttggcaaaa aacgctgata
    10741 attgagattt tgctatcaaa tcaaaggttc caaatttacg cacatcttgg taaaccaagg
    10801 tcgaaccatt tttcaattca aaaaagacat gaaagtgttt gttatcaggg acctcatctg
    10861 gaaaaagcaa atatttgcct tccatccgca aatgtgatac caacactaat tgccccaaat
    10921 caattaataa atattttccc cgacggccaa ctgactgaat gatttgacca ggtagggtca
    10981 gggcaaacgt ctctaagtcg gttttaacca tttttggcac tttaagcgta acagccacaa
    11041 tttcttggcc aagcactaaa gcctcaagcc ctcgtcggac cgtttcaact tctggtaatt
    11101 ctggcataat ccgttattta aaggacagct agaccctttg ctgctacaaa gaataaactc
    11161 agcataaaaa agtcactcaa tagactcagg tctaggatac tgactgcgtt tcaaaaaaca
    11221 cagctgtcct tctcctttct tatttcttat tttacgaaca catctgatag aaagcaactc
    11281 ataaatctgc cccagaccag tcctcatccc ccttacctcc cagcactatt tggcggtaat
    11341 gttccttata actataatac aatgagtcct ctccaagata cttaaaaatc tgcaaggcct
    11401 gccgcatgtc ctcttctcca gaaatttctt tctgctttaa tttataatac ccatgcacat
    11461 ataagaaaac agtcttttca taaaaattga gctcgtctcg cagcaaaaga tcaattttgt
    11521 taatcaaata gcgactatgt tcaaagcaag aatggtcaat actgataatg agacaattaa
    11581 tggataactg agtcactaac attttattgg ttttattttg ttccgaataa gcaaaagatg
    11641 ctaccatttc cttagtcaat aaaaataggg tattgtaatt catgaagcgc gagcaattcc
    11701 ccagaagaat gatttcataa tatccccact gttccacttt aaataaataa tctgtgaggc
    11761 gagtcaattc ttcttgacta ggtgcaattg aggagtcaat agaaaaaaga atagctttaa
    11821 tcatcgttcg ttcataatct ttatgggcat aatccttcaa cagtttggtc aatttaacca
    11881 cgtttttctc agcataacat tttctcgcct gacttaagag cgtgaaaaaa tgcgtatgcg
    11941 tcttggaatg agccgaaaca aattcgtcaa tcgtaatatt cagtttatcc aaaagattaa
    12001 gcaagcgact gcaggtgatt tctgattccc ctcgttcaaa tcttgaaatt tgtgacttgg
    12061 acaggtattc atctgctaaa aaagaaatgc taacctgctt acccttacgt aatcgcctta
    12121 aggtttttcc gagttctttt tccatttttc ccactttcac aacaaaaaat taaaatctgc
    12181 taaatccatt atataataca agtacaaaat agaaaatcag aaaggagaca gtcatgaaaa
    12241 aagttaataa agctttgctt tttacattga tcatggatat cctgattatc gttggtggat
    12301 aacctttcgc ttaaaaacaa tccacttttg ggaaaccctt atatttagtt tgtaacagga
    12361 aaactaataa atattaaaaa gagtgttagc gacctagcgg cagctttact cttaagtgcg
    12421 tttgctgcca ttgacagcaa ggtcaaagct gatgaaacag caggggcata ccgttatgat
    12481 gtgtctgaac aaaacactta ctatcaaact atcagctcca ttaaccagca acgacctggt
    12541 gccactctcg acgaatgagg tgattattat ggaaccctta cttcatttac ttcccaaagt
    12601 cgtaccagta cttatacact tggtagacaa agccaacgta tcatggttaa cagtgatatt
    12661 acagttacct atactcttaa agacaatttg gtagcccttc ataaggaata taaaaccttt
    12721 gtcaaaaaaa tattacagat aaaccgatca ccagtgacta cgacaaggct aaggttattt
    12781 ataaccattt aatcaacgaa tacacttacg ccacaaaata acttagcacc aatcgcaaaa
    12841 tcgtaagcag catcaacaat catactccta aagctcttta tcgtggcaag tgtggtgttt
    12901 gtcaagctta tgccgtcatg tttaaagata tggcagaagc tgcaggctta tcagcttggt
    12961 atgtgataaa aaaggattga atgagcacca tgcttgaaac atcgttacca ttcatggtgt
    13021 taattcctac gttgatgcta cctggaatga ttcagcatac accacaaggt atttccttgc
    13081 tggaaaagac gtgaggaata ctgagtaccg tttagatagc cagtacaacc acttggctaa
    13141 tgctatttta actaccagct accaaaaagg ctaaactaac aacaactaca tttttcaact
    13201 agaaaggcct aaacaatgac caaaaaacag ttttatctta tcttagctat tagcatactg
    13261 tgttttattc ttgccgtcag ccctaaacca ccaattccta aacacactat tgaaaggaaa
    13321 aggactgcga tgaccatagg ccactcaaaa caaaaccctc acaaaaaaga agcccttaaa
    13381 cacaaaaaaa acaaaaataa gactcttttt cttccaagtg gggacagcgc tatcaaatct
    13441 gttattagca tgacagttag actcctgtta gatgaccaat cttagctctc ctaaaatcag
    13501 tggacactac cgtttcggta gtgttctttt ttaagctaat ctcatcacca cgctttcttc
    13561 aagctaaaaa actgatcatg aaagagcaac cccaccaccc taacagccca ctttgcccgc
    13621 actttcttaa aaagttagga gaaaacgata taccaattat tttctgatcc cagctcccta
    13681 tcattaacaa gatgtgtagc acggacctac atacaggtaa gctagagtca ttctcgctat
    13741 ttcggacaaa aagaggccgg tagttccctg acctcttctt tatttatctt gtaacactcc
    13801 tcttcttagc gagacttact agcacataat tcttaagcac tcttatcatt tagactagga
    13861 agaataccat ctgaatgaag caaatgatgc gtcagatctg ccagagtatc atctccataa
    13921 gtaatccctg tttggtgctg tttctgatga aaatcagatg actccttaac tttagcagtc
    13981 acctgctggt cacttggatg aaaattaatt tctctcttgg cttcagccgc aactggctcg
    14041 aggaacaact tattgacatt acggtcttcc tccaaatgat taaggatagc agcatcatct
    14101 tctaacgaaa gaaccttctt gggtaacaaa aaggggttat ttagccttgg ctcagtaacg
    14161 acttccttgg ctgctcgtga tagtttatta ttgtcttcct caggtatcaa acgattaagc
    14221 aatcgatgca attcttccgg ctatgaggcg ctcacagcta cttcagaaga aacctccaag
    14281 ccctttcctg tttgcctgtc taaagcaacc ggcaaattat taactaactt tttaacctgt
    14341 cctcagcgct catttcaaca ctaggaagag ctgtcgctgg ttctagccag ctaccaactt
    14401 ctgctggtct taccttaaca tcgctggcag gcttctcctt ctcatcttca ttaggtctaa
    14461 aaggctcaaa ccgccctgtt gatttttcat ctatgacctt tatattgtct tttactacat
    14521 gagacagtgg ttcgacattt accatagctt catgctcgtt aaggctctgg tctcgtttga
    14581 atttaccagg aataccagga gtcggtgttc caacagattg atcggtttca gtaggctctt
    14641 ttacggagcc ttttttccat aaaactgctg gaggaataat atccgtagag ccatccccat
    14701 aagtaatcgt ggcctgcctc ccctttggat taatagatac tttaatattg tctgggaaag
    14761 ccccactacc tcctgtggtt aaaagggcac ccgcattttt aatgagagtt gtcattcgtt
    14821 caataccagc aacgccaata tggttcaagt catctactgg taactgatca acagatttca
    14881 tggtgtattt ccaagcttgc ggttgaacaa gaaaaacagt tcctttatac attgtgttat
    14941 acatatcatc aatgactttg aaataccttg tatatttggt ttgtacatta gggttagcaa
    15001 cgggaacagt gctttcagga actcctgaca tcgtaaaaat ccctctagca gaagtttgaa
    15061 agccctcata aacatgaccc ataccaggaa cagtctgagc ggtcaaagac ttaccagaac
    15121 catccaaagc aatgcgaata tctttcatca gatacatctg attggtgtag gtaaacctaa
    15181 ccgtgttagc ctcacctcga tacaaaacca cttgggaggg ctgatccgaa gaaaactgag
    15241 aggttgtttc ttccttatag ccaactccaa aacgtctatc agacgaatgg actgaatctg
    15301 ccgcaactgt actactcaaa ctgactagta aagtagccgc acaaccaact aataaactgc
    15361 tcattttaac tttaacatct cttaacatca tcatgttcct ttcattattt tttatttagg
    15421 aattcgtcat actatatttt ctcatattag tctaacaaaa aaaccgtaat tctatataaa
    15481 tcatataaaa tgacgagctt tggcaaagat gagtatagca ggatggaaaa cagattgttt
    15541 ctgaaacttc aaaggtatct tcttgtcacg aactattctt taattgaatc ggttacacta
    15601 aactagtccc attatttaaa aacaaacttt aaatattatt cattt
//
LOCUS       DAUELZ010000040        15502 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.040, whole genome shotgun sequence.
ACCESSION   DAUELZ010000040 DAUELZ010000000
VERSION     DAUELZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15502
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.040"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..724
                     /gene="ropB"
                     /locus_tag="VHI09_000943"
                     /gene_synonym="rgg1"
     CDS             <1..724
                     /gene="ropB"
                     /locus_tag="VHI09_000943"
                     /gene_synonym="rgg1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="RopB (Regulator of Protease B) is a paralog of the
                     quorum-sensing transcriptional regulators Rgg2 and Rgg3,
                     which bind short, hydrophobic peptides encoded by genes in
                     their respective vicinities. RopB binds the leaderless
                     short peptide SIP (SpeB-inducing peptide), with sequence
                     MWLLLLFL; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     RopB/Rgg1"
                     /protein_id="HEQ6120827.1"
                     /translation="ISSKKLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFE
                     CRNIEGLNHILDSYKDSKSIKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETW
                     SHYETVLFNNCMFIFESCFIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQR
                     QEYDKASEILAKIEDYQLNDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQL
                     LNCKTIHHMFQTYLEAIKHKLS"
     gene            complement(965..1780)
                     /gene="sdaB"
                     /locus_tag="VHI09_000944"
     CDS             complement(965..1780)
                     /gene="sdaB"
                     /locus_tag="VHI09_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEQ6120828.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSIGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            2144..2653
                     /locus_tag="VHI09_000945"
     CDS             2144..2653
                     /locus_tag="VHI09_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEQ6120829.1"
                     /translation="MSTDHHLREVSYQLLAERGGSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGNSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            complement(2735..3823)
                     /locus_tag="VHI09_000946"
     CDS             complement(2735..3823)
                     /locus_tag="VHI09_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEQ6120830.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGDSPILLCDDVVYGIVG
                     ERFESYLIDNGMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMAGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(3880..4548)
                     /locus_tag="VHI09_000947"
     CDS             complement(3880..4548)
                     /locus_tag="VHI09_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ6120831.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYEGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAINRENA
                     DSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            complement(4561..6978)
                     /locus_tag="VHI09_000948"
     CDS             complement(4561..6978)
                     /locus_tag="VHI09_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEQ6120832.1"
                     /translation="MTETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKDVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGYGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGRLTPEELWQSLESDFAGERGEEIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            complement(7189..8493)
                     /locus_tag="VHI09_000949"
     CDS             complement(7189..8493)
                     /locus_tag="VHI09_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ6120833.1"
                     /translation="MAKMNMQNIIMPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSGVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEPIGDAISKAWFGGQGIIGAIVIGLTVGAIYTAFIR
                     RHIVIKMPEQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            complement(8503..8811)
                     /locus_tag="VHI09_000950"
     CDS             complement(8503..8811)
                     /locus_tag="VHI09_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ6120834.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIDAYSQGKLAE
                     YAPLIDVALLGPQVAYTLDKSETICKENGIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            complement(8839..9159)
                     /locus_tag="VHI09_000951"
     CDS             complement(8839..9159)
                     /locus_tag="VHI09_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ6120835.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            complement(9446..10426)
                     /locus_tag="VHI09_000952"
     CDS             complement(9446..10426)
                     /locus_tag="VHI09_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEQ6120836.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRKQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            complement(10442..11188)
                     /locus_tag="VHI09_000953"
     CDS             complement(10442..11188)
                     /locus_tag="VHI09_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ6120837.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKIITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSASGLVLDFVS"
     gene            11314..12087
                     /locus_tag="VHI09_000954"
     CDS             11314..12087
                     /locus_tag="VHI09_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEQ6120838.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            complement(12324..12500)
                     /gene="secE"
                     /locus_tag="VHI09_000955"
     CDS             complement(12324..12500)
                     /gene="secE"
                     /locus_tag="VHI09_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEQ6120839.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(12514..12666)
                     /gene="rpmG"
                     /locus_tag="VHI09_000956"
     CDS             complement(12514..12666)
                     /gene="rpmG"
                     /locus_tag="VHI09_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ6120840.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            complement(12715..15051)
                     /locus_tag="VHI09_000957"
     CDS             complement(12715..15051)
                     /locus_tag="VHI09_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ6120841.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     SLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            complement(15090..15470)
                     /locus_tag="VHI09_000958"
     CDS             complement(15090..15470)
                     /locus_tag="VHI09_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ6120842.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
BASE COUNT         4318 a         3205 c         3097 g         4882 t
ORIGIN      
        1 catttcttcg aaaaagttac tttatatctt agacaacttg aatgtcaatg ttgatgagtt
       61 tctctttatt agtaataact ttaaacaata caaagaattt attgatatgg atacggcaaa
      121 gcattatttt gaatgccgaa acatagaagg tttaaatcat atccttgatt cttataaaga
      181 tagtaagtca ataaaggaaa agaacctttt tgccttggtc aaggtgttat tagcaactct
      241 tactgaggaa gactgtctga cagagcggac ttatttgtca aactatctta ttaatattga
      301 aacttggagt cactatgaga ctgtgctttt taataattgt atgtttattt ttgagtcttg
      361 ctttattgag atggtgtttt caaaagttat tttgaacctc gataaataca ataccctaag
      421 gtattatggg aatgaatcga ttcggatgtt tgtcaatatg ttgattttgt ttattcagcg
      481 acaagagtat gataaagctt ctgagatttt ggcaaaaatt gaagattatc agctaaatga
      541 tgattgctta tatgaacggt gttgtgtgtc tttttttgat ggcattattg gcctcataaa
      601 tggtaaagag ggggctgagc aaaagtgtgt gcagatcttg gagatcttcc aattacttaa
      661 ttgcaaaacg atccatcata tgtttcaaac ctacctagaa gccattaaac ataaactgtc
      721 ctgaggctct ttaaaaagct aacgctctta tggtgttagc tttttgattt ggtatcttaa
      781 taaatggtgt agtttatctc aacagctcca gggcgatttt ttcaatttgc tttttacttt
      841 ttgacaaatc ataaaaagca atgacggcaa tcattgcttt ttctagtatt tttactttct
      901 atagtcattg ctttttttga tgtaaaaagc taggcctagt gagcagaggt ctagcctttt
      961 ggtattattt ctgagtaggt gtaccgttat ggtagttaat ggtgtagcca ttagctgtgt
     1021 tgtaaactaa tactttctcg ttgatggtat tatcagaaga ttgcattgat accacgacag
     1081 ctcttggaat caactcgtct gcgttgtaga ttggagcggc ttcataataa agatagccat
     1141 cacgatttgc ttctaaccat tcttgagctc tttgttcggt atagcgcatg ccgccttttt
     1201 ggtcacgacc tcctacattt tgggtacgtg ttcctgtaac ggcattgact ctgagtgcat
     1261 ctccaccgat actatctgca atgagatgac ttctattcca gaaatctccg acataagaca
     1321 gaccatttaa ccattcaatt ttatatttga catgattagg gtttccagtc cagcctgcgg
     1381 ggttttgatt tttaccgaaa gattgtctaa caccgtagct accttcaaca ttggcataag
     1441 tcaatgtacc tctagcagta cgcgtccttc ctaactcatc taatttgcta tagagaatat
     1501 ctcctgcttt aggaaagagt gctggagtga tctgactagt acctaaggtt ttgtaatagt
     1561 tgggactgtc attgaatgtc caagctaatg cttcgtttag gtacttgctt gcgccatcat
     1621 ttagaacaac atcatttgag acctgtgttt gtcgtgccag tgcagtattt tcaagtgtga
     1681 ctgttgttac agccattgtg gctgatacaa gagctaccat tgaaaatttt actagccgac
     1741 attttttaga aaaaacccgt cttgatccaa gtagattcat atgcttgtcc tcttttctaa
     1801 tttcgctcac taaccatagt atacgcttac ttaactagtt tatcaatagt ttttttaaaa
     1861 aatatgaaaa ttaaaggaga tagtaaggag aaaaaagaag gcgttgtctt tttgttttgc
     1921 catactaata tcatttttga aataggtgct gtgtgtcacc taggggtggt gattgtattt
     1981 aggcctaggt aagatggtta ctttactatc gtttctttga cttttaccaa gtaggggatc
     2041 tcagtagtca acatctctta aaaaagaact atcccctctt tagctgttgt agaggtttct
     2101 cgaaatcttt tatgttacaa taaagaaaaa aaggaggtct cacatgtcaa ctgaccatca
     2161 cctacgagaa gtgtcttatc aattactggc tgagcgaggg ggttctttag aagctattgc
     2221 agagctggtg ctttttttgc agaatgatta tattcccaat ttgaccatgg cagaatgttt
     2281 agagagcgtt gaggcggtgc ttgcaaaacg agaagtgcaa aacgctatta tcactggggt
     2341 agaattggat aaattggctg aagccaacca gctgtctgag ccactcctta gtatcttgaa
     2401 aaccgaccaa gggctgtatg ggattgatga aatcctggcc ttatccattg ttaacctcta
     2461 tgggtctatc ggttttacca attatgggta tctggacaag acaaaaccag gaattgttga
     2521 caaactcaac cataaagatg gtaactcctg ccataccttt ttagatgata ttgttagcgc
     2581 cattgctgct gcagctgcca gtcgtattgc ccacaatgac cccgctaaaa gtgctattac
     2641 caaccaacag taagcaaaac aagtctagga agtgactccc tagacttgtt tttttgaggt
     2701 tatctggcaa ataagaaatc tctttttggt tagcctatcg actcgtaaca taggcatcaa
     2761 cagcaaccaa agccgctgct acatcttctg gactgataac aaatggcatt tgatggatgg
     2821 tttcgcctgc catggttgct tgacggccaa tttttaagaa atcctcttga gtagccgtgt
     2881 ctaaatgcat ttctttgagg gttgttggca tgccaatagc ttggtaaaag tcgatgtagc
     2941 ggtcaatttc ttcacgcgaa cgattttcca agaagagttg ggtcaacgtg ccataggcga
     3001 ctttttcacc atgggtaagg tgatgaatag cacctgtcag cgctgtaaag ccattgtgaa
     3061 tggcatgtgc agcagccaga cccgcacttt caaaaccaag cccgctaagg agtgtattgg
     3121 cttcaatgac attttccaag gctggggtca cgacttgtct gtcacagcta gccatcgctt
     3181 ttaggccatc tgcaaacaag gtctgctcac aggctttggc aatggcaact cctgccaagg
     3241 tttgattgcc acctgccatg gtgtctccat ttttttgcat aacagcacgc gcctctaccc
     3301 atgttgctaa tccatctgca atcccagagg ccagtaatcg ttttggtgcc tgacagatca
     3361 cttgtgtgtc aaccaaaaca agatctggat tttttgaata gaaaatgtat ttttcaaagg
     3421 ctccttcatc ggtgtaaata accgataagg ctgaggttgg ggcatctgtt gaagcaatgg
     3481 ttggggcaat aatcaccgga acagcaagta agtcagcgat agccttagca ctgtcaatgg
     3541 tttttccacc accaagtcca ataataacat cattgccatt ttccttggca atggcaacca
     3601 cgcgactgat ttcattatct gaggcttcgc cattaaaggc tacgtgaaca ggagtcatgc
     3661 cattatctat caaatagctt tcaaatcttt ctccgacgat gccatacacc acgtcatcac
     3721 acagtaaaat ggggctatcc cctagttgtt ttagggtttt agcattggta aacaaggcat
     3781 ttttaccttg aatgtaacga gatggacttg caaatacttt catcgttgaa ctcctttatc
     3841 tgtgattcct aatgatgtga ttactactga tgctatttct taaatgctct tgcggtgatg
     3901 aatggcttcc caatccttgc caaaatcgtc aaccgctttt tgaatagatg gcatggcaaa
     3961 tcctgcttca aacacatctg ggccagcagt gatggcttgt gctcctagag caaaggattt
     4021 attaacctga gccacatttt taaagcttgc tgccaaaatc ttactatcag cattctcgcg
     4081 gttaatagct tctgctaatt gtcctatgac agcttctgga tcaatattga gattttccat
     4141 acggttatag taaggagcca aataatcagc accagcttca atggctaata ggccttggaa
     4201 ggtcgtataa atagcggttg ctgtgatatg atagccttcg gctttcaagg ttttaatggc
     4261 tgcaaggcct tctgtggtta caggtacttt gacatacaca ctatctccac attgcctgcg
     4321 gatttcagca gcatctttta agatgccttc gtaatcttgg gcgatcactt gaacatgaat
     4381 agaagccttg tcaccaataa tagcacggac ttctcgaatc cgttcaaaga aatcaatgtc
     4441 accttctttt ttggcaatag aagggtttga ggtcacacct gcgagaggca aaatgtgatg
     4501 ccatttttta atagcttcta aatctaaagt gtctaacatg tattccatac ttattttctc
     4561 ctaaagtgtg tgttctgtac gttcaataat gtcatcttgg gttgcttttg agagcacatt
     4621 gaagaaagct gagtagccgg caacacggac aatgaggtcg cggtgctttt caggatgttt
     4681 ttgggcatca atcaaggttt cacgtgacac gacattgtac tggatatgat aaccgtgtag
     4741 gcgattaaag aaggtacgaa gtaaggccat taatttcagt ttgtcttctt ctttggccag
     4801 ggtttgtggg ttaacttttt ggttgagtag tactcctccg acaatctcat ctgttggtaa
     4861 tttagcaaca gatttcaata ctgaagtagg tccttttttg tccatgctgt gttctggtga
     4921 acagccttct gctaggggtg tccctgcatg gcgaccgtct ggtgtcgcta aggttccttt
     4981 tccttgtccg acattggctg agattgatga tgtcccagaa tagcggatac ctccgattgg
     5041 gccacggcca taccgggtat ttgggtattt ggcaatttca tcaatgtagg tgtcgtaagc
     5101 ttcgacaact aaactatctg cgtagtcatc atcgttacca tattttggtg cgtcattgat
     5161 gagcatttgg cggatttctt ctccgcgttc tccagcaaag tcactttcaa gggactgcca
     5221 gagttcttct ggggtcaagc ggccttcttc aaacactaat tttttcagcg ctgctaggga
     5281 gtctgatagg ttggcaattc caacttgaag gcctgagata tagtcgtaaa cagctccgcc
     5341 ttcttttaag gtcttgccac gaccgataca atcatctgtc aaggctgagc aaagaatatc
     5401 tggcacttca cgttcaagac ctaggtcaat ggcattttca acaatgacgc tcatgcgggt
     5461 aatttcgcgc aaggtggcat cccatgctgc ttttaattct tcataagagg tcatgtcttt
     5521 gaagtgaccg tatccttttg caaagcgctt gcctgatgct gggtcaatcc cgtcattcat
     5581 ggtaatcagc aagattttag ggaagttgat gtaactcatc cccgtgcaac ggtagcccca
     5641 tttgcctgga accgctgttt ccacacagcc aatggctgag taatcataag catcttcttc
     5701 tgagacatct tttttgatga aagatggaat gatgatttca tcgttgttca tggctggcat
     5761 gccaaaacca agtttcatga cctcgataca ctcgttcata aaggtattgt ctaggccttt
     5821 gtggtaacga acagtcaagt taggttgtgg caatttggtt tgagccacac tccgtaggac
     5881 aaggtaagac agtgggttga ccgcatcttt tttatcgcgt gtttgaccac cgatcgtcac
     5941 gttttggtaa agtggactac ctgctgatga gaaggtgtgg gcttggctcc gcactttgtt
     6001 aatggtcaag gtcttaatcc agaggttagt caaacgttca acgattgagt cttctgtttc
     6061 acggccagcc tccaaatcag ctttgacata aggatacatg tactggtcaa agcggccata
     6121 agatagggaa tgcccgtttg attcaatttg caagatacac tgaatgaacc agacagattg
     6181 cacagcttct gcaaaggttt tcgctggttc gtaaggcact ttgtcacaaa tacgcgcgat
     6241 gtctaacaat tctgcttgac gttctggttt agccgttttt gctagttctc tagccaactt
     6301 ggcatagcgt ttggcataag ttttgactgc atcaatgaca ataaaaacag agtcgtaaaa
     6361 atggtatttg tcaatgtttt caggaatggt caaatcaagc gcagctttgg cagctcgtgc
     6421 ccgttcttca aatcccttta agccgtgctc caagagtttt tggtaattaa ccgctaaatg
     6481 ggcatcccca gagttcatct tgccttccat gccaaagaaa cctgtttcca tgtacacttg
     6541 cacttcttct ggtagcaaga ccccgcagcg agcacgtaag ttattgtttt cccagaatgg
     6601 ggcaatgtct cggagctgtt gcttggtttc ttcggtaatg tagaagacat ccccatctcg
     6661 tttttcaaaa agatcaagtt cgttgaggac aaattccaga gtgtattctg ggaaaatcgg
     6721 tgcatccttg tttgaagagg cttggttacc agcaatgagg ctttcatcct caatatagat
     6781 ggtcatgttt tccaaaatag tttgaagcat gtaagcgcgt ttgaggtttg ctggcttatt
     6841 ttggtgtttt tgataggctt cagtggctaa aatagcacgc tcagcatcaa tataaggttt
     6901 cttgtctaac acagcttcac gatagtgagt cattctgtct gttaaatgtc caaaataagg
     6961 gctttttgtt tcagtcatgc ggggtctcct tctttcttac gaaattataa atgtttcttt
     7021 cgtaactatt ttatcatata ttgcgacttt tgcgtatcct taaccataag aagagatagt
     7081 cactttattt tgcactattg tgcaatatag gcctgataag tcgctcttct ttttctaaaa
     7141 aaccacctct aactggagat ggtctcaaga atttgtgtgg acgctaaatc atgactcttc
     7201 actagcttgt tcattttgat aagccatatt atcttgaatt ttaaagaatg ggaagtacac
     7261 caacgttgac atgatgagta taagaatttg cacaatagct ccttgccagc caccaaccat
     7321 gaaccctgag ataatggctg gtgttgacca cggaagggtc actcctgcaa agggctgcat
     7381 gaaaccaata gcaatggcgc cataaactgt gagagctgcc aatacaggaa ccaagataaa
     7441 tggcaagaac atgactggat tcatcacaat tggaaaacca aagacgacag gttcattaac
     7501 attaaagagg gctgggaagg ctgcaacctt acctaaagcc ttgtattgtt tggatttagc
     7561 cgcaaagatc atagctacca ctaaaccaaa ggtaatgcca gaacctgata gaatcaagaa
     7621 agagtctaaa aattgttggg ttacaatatg ggcacccttg tctagggata actcacctgc
     7681 tgccatcaag gccttattgg catctaaatt tgataagaga agagcagtga caattccatt
     7741 gaccacagat tgcccatgca cgccaaacca ccaaagaaag gaaataaaga aggcaatacc
     7801 tagtgcgcca taaagagaac ccgttaaccc ttgcagcggt acctgaatga catcataaat
     7861 catttcaata aaggtgccac cacctgtcac tgacttggca ataatgtaca caagcattga
     7921 caaggtaaaa atcacaaagg ctggaatcat agcttcaaac tgtttggcaa tggcttgtgg
     7981 cacttgctct ggcattttga tgacaatgtg gcgacggata aatgctgtat aaatagcacc
     8041 taccgttaaa ccaatcacaa tagcaccaat aatcccttgc ccaccaaacc aagccttgct
     8101 aatagcatca ccgataggtt cgccttctgc tggaacataa gatgatctca ataaaataaa
     8161 gaaggctgac aaggatagaa cccctgaagg gagaggttca acacctgagt ttttagcata
     8221 agaatagcca attgcaaaac aggaaatcaa ccccataata gcaaatgttc catggtaaac
     8281 ctgcataaag ggttctgtcc agtcagcacc aaacacacta gcaatagcat cattaacccc
     8341 ttggaaagga atctgcccag caatcaggaa aagactcccc acaacagtca aaggtaaaat
     8401 ggctaacata ccatctttta aggcaatgat gccgcgcata ttcacaaatt tcatgattgg
     8461 catgatgatg ttttgcatat tcatttttgc catgattgac tcctattctt taacaaggct
     8521 aagggctaaa tcaagcacct tgttaccgtc taacattcca taatcagcca ttgggataac
     8581 tgcaatcggg atgccattct ctttacagat agtttctgat ttatccagag tgtaagcaac
     8641 ctgtgggcct aaaagtgcca catcaatcaa tggagcatac tcagccaact ttccttggga
     8701 ataagcatca atctggcagt caatgccttt cttttcagca gctactttca tattattcac
     8761 caacatgcct gttgagaacc ctgctgcgca aaacaatcca atcttaatca tgtcaatctc
     8821 ctttttatga atgaaatatt atttggttgc caattcttta cgaaggtcaa taatctcctt
     8881 gatgagatta atttctgtaa tggtcgtcat caagtgatct tgtgagtgaa caaaaagagc
     8941 tgtaatctct gacttattac cactagcttc ttgggcaaga aactgggtct gtaaattgtg
     9001 ggcttctaat aaggcatcat ctgctaaggc catctctttt tcactagtgg cataatcgtc
     9061 ctctttagca catttcagag cttggtagat atgttgtttg gcatcgcctg catttaaaat
     9121 taagcccata atgatttggt ctggtacaat aacttgcatt ggtgtctcct ttttttcttt
     9181 tttatgtgat gtaacggcaa tgagtctttt tttcgaaggt gatgttattg tgtttgtaat
     9241 cgtttacatt tcgttggtaa gtttattgta tcatttttgt ttttagggtc aatatatttt
     9301 gtgctatatt ttggttaatg cacttttgtg ccaatatatt aactgcacat ttgagcaatc
     9361 tctgtttcat caacacatca aacaaaaaaa gccctgacgt ttggtcttgg gcctttataa
     9421 atatcatagt atctttgaat ttctattaaa gaagaacgtt attaagatca atatcttcat
     9481 ctaacgctaa aacagctagc atggtctctt tatccgtcac tagataattg ataaaacctg
     9541 cttttaaggc tgccaatatt gccttagcct tgtgtttacc tgttgcaaca gccattgttt
     9601 tggggacacg cctcaactgt tctagcgaaa taccaatagt tcgatcctgt aggccagtgt
     9661 aaactggctg acctgcctga tcaaagaaac gacaacaaac ttcaccaacg gctttttcat
     9721 atttgagttg gtcatgatct gagctggtta ataaatcacg ccattggctc tgctcaaggc
     9781 tattaggctc cccaccaatt cctactaagg caagatctag ctggtcccag cttgttaaaa
     9841 tgtcgttaaa gtatttagat tgtaagattc ccttagccaa gtgtttgtct tcttgaatga
     9901 ccatagcgtt cataaaagcg ctattaccat gaaagatgcg agccaagcgg taaaccaggg
     9961 tattgacatg gtatttggca ttaatatggc taggtcctcc tgccaaagga taaataaaca
    10021 catctttcat cgcttttgga tttaactcat ccatcaagca agaaagagtc gctccccagg
    10081 acagaccgat cttatcgcca tctttaacaa catgtctaaa aacctcagct gctgttttag
    10141 caacatttgt caaggtatcc attggggtat cttcaacctg gttgggcaca aggtctagtt
    10201 tttccaagcc atactgcttt cgcacgtatt cttctaaagc aaataaatca gcatcatagt
    10261 cggcaatttc aatccgaaca atgccttctt ccttggcttt tgctaacatc cgacaaaccg
    10321 ttgttcggta gatattcatt tcctttgaaa tcaaggtttg gctcttgcct tgaacataat
    10381 gcaagtaagc caccttggca aggagccgtc tgcgttcttc tttcatctag ccacctattt
    10441 tctaagaaac aaaatcaaga acaagaccac tagcacttaa taactcttgt gtttgcttgt
    10501 ctagtgcttg gtctgtaatg acgcgattga catcagctaa tgacaactgg tgtaccagac
    10561 tggtttttga aaacttgctg gaatcggtca gaatcaccac ttcctcagca gcatctgcca
    10621 tgtaacgcac cccttctgag cgcatcatgt ccttgcccat aaagccaaga tctttgttga
    10681 aaccgtctgt tccaacaaag acacgattga catgaaaaag ggatatcatt tcttttaata
    10741 aaggaccaac agtcacctct gaattgggct ggtaataacc gcccagtaaa atgatttgac
    10801 agctactata ttgacgaatg tagttggcaa tgaagcacga attggtaatg attttaatat
    10861 ttcgtttggt ctggcaaagt acttctgcca aaagggcaca ggttgagcca gattcaatca
    10921 taatggtatc gttgtcttgc accaattcag ctgctttttc tgcaatccgt cgcttaatgt
    10981 tgtaattgta ggacaagcgt acattgaggt catcgccact gtttagcacc gcatagccat
    11041 gttcccgtcg caacaagccc ttactttcaa gcttatcaag gtcttttcga atggttactt
    11101 tcgaaacgtc taattgctct gataaactgt taacatctat ttttttcttc tgggaaacca
    11161 gttgaataat acgttctaat cgattcatca ttcacctccc tttcattgta ccagttattt
    11221 tcttaaaaga aaagggatat ttactttcat tcgtaattga tttatttacg tttatctggt
    11281 atgatggttt tacaacgaaa ggaattcaca cttatgactg atcgcggtat tgttttcaac
    11341 atccaacatt ttagtattca tgacggtcct ggtattcgga caaccgtttt tttaaaaggt
    11401 tgccccttga gatgtccatg gtgtgccaat cccgaatccc aacaaaaagc gcctgagcag
    11461 atgttaacaa gtgatgggct taatactaaa atagttggag aggaaaaaac cgtggacgag
    11521 gtgatcgaag aagtccttaa agaccttgat ttctacgaag agtcaggtgg gggcatgacc
    11581 ctgtcagggg gggaaatctt tgctcagttt gactttgcct tagccctgtt aaaagcagcc
    11641 aaagcagcag gtcttcacac cgcaattgaa accaccgcct ttgccaaaca cgagcaattt
    11701 gtcaccctta ttgactacgt ggactttatc tataccgatt tgaaacatta taatcagctc
    11761 agacaccaaa aagtgactgg ggttcgtaat gacttgatta tcaaaaatat tcattatgcc
    11821 tttcaagctg gaaaagaaat cgtcttgcgc atccctgtta ttccccaatt taatgattcc
    11881 ctagacgatg ctaaagcctt ctcagagctc tttaatcagc ttgagattga ccaagtgcaa
    11941 ctgttacctt tccatcaatt tggagaaaac aagtacaaac ttctaggtag ggagtatgag
    12001 atggctgagg tcaaggctta tcatcccgaa gatttagcag actatcaggc tgttttcctc
    12061 aaccataata ttcattgcta tttttaatgt gctaagcaag tgaggccagg ctgtttatgc
    12121 gttgactctg ttacaagcta atttagtgca cttggtttgg agatcgttgc tacattgcct
    12181 ttacctttat gcttttttaa agctcaaacc agtatgggct ttaaaaaagc ttactaatca
    12241 gattattgat actacaaaaa aggctgggaa tactcccagc ctaggctttt ccaagcaagc
    12301 ttatcatttc taccaatctt gacctagaat aaattaatca gtgctaatac actctttgct
    12361 aagagctggt caaagatgta gataatcacc gtaaagaaag ctgtgtactc aagaactgag
    12421 ataaaatctt tccaacgttg tttacgattt ggccatgttg tgtcttttaa gactttaaaa
    12481 gtcccgctaa tgaatcccat caggctctcc tttttaacgt gtttctttgt gtaaggtata
    12541 tgttttgcag tatttgcaaa atttatttac ttctagacgt gttggttttg gcgtgctact
    12601 cactccgata gagtagttac gactgccaca ttcgacacac gctagactcg cttttttctg
    12661 tgccataacg cctccgtaac tattatttta acacgatctc taaaatactg caagctatct
    12721 aaaatagtca acaatctctt tccaaataga ttgggcctta tcacgtaacc ctgactgatc
    12781 gactgctttt gatagtgatt gcgaagcttc ttgtgctgat tttcttaaac tatcaataat
    12841 agattgagtg tcaactcctg attgattacc tgtttcattg actccataaa cagctgaaat
    12901 accattttga gcgtaggcat tatctacatg aaattggctg cccttggtat aaggcaaaat
    12961 gtaagatgcc tgagtgctaa aaatagctga agccgtgcct gcacttgaat ccgttagata
    13021 atgattttca tcggtctgat taaatcctac ccattgacta ataacaacat ctggcgtata
    13081 accaataacc cactgatcgc ctgctaagtc gggattgaag ttggtttctg tcgtccctgt
    13141 tttaccagct agtgtatagc catatacgtt agcattgact gctgttccat ttgaaaaggt
    13201 acctagcatc atggctgtca tcttatctgc aacagactgg ctgacaacac gtttagcctt
    13261 atcagtaaag gttttaagca cttcacccct ggcattttca atccggttaa tcaagtgcgc
    13321 aggatggatt actccattat tggcaaaggc agcatatgcc tgagccatct ccaatggatt
    13381 ggttgtcacg ctgccaccta aagctacccc caactctttt ttggcagagc tcatatctaa
    13441 cccaaatgtt ttaccatagg taaaggcttt atcgattccg atatcgttca atgtagaaac
    13501 tgctggaata ttataagagt ttgctaatgc ttgatacata ggaacatctt ctgattcata
    13561 atttccataa ttatgtggct gatagccatc gaaatcttga acggtatttg gtagttcttt
    13621 ttcaattgac catcctgaag caacggctgg cgcgtaaacc acgagtggtt tgattgttga
    13681 tgcgggacta cgttttgctt gagtcgcata gttaaaactt ctgaaagtcg gattttcact
    13741 actattcaca cgaccaatca gacctctaac acctcctgtt ttaggatcta aagcaacact
    13801 agctgcttga gcagagctac cgtcataagc tgaaacagga aatagttcat cgttgttaaa
    13861 agtcgtctgc atgccagttt ggtaattttg atctagctcg gtgtaaactt tgtatccatt
    13921 attaacaatg tctttttctg aaagaccata agttgctatt gcttcactaa taacagcatc
    13981 aaagtaggat gggtatttgt agtcatctgt cttaccaaca taagtatcag ctaagcgatt
    14041 ttttagccct actgctctag cttgttgagc ttttgtttgg gtaatctttt tggcatcaac
    14101 catcgctcct aaaacagtat ctctacggtg agtagcattt tttagagaat ggtaagggtt
    14161 atatatttca ggtcctttga gcatacctgc taatgtggca gcttcatcca atgttaagtt
    14221 agcagctgtg gttccaaaat atttttgact ggcatcttca actccccaaa ctccatttcc
    14281 aaaataggag ttgttaaggt acatagtaag aatatctttt ttactgtatt ttttggtcaa
    14341 ctctaacgcc aaaaaaaact ctctggcctt tcgtttaatt gtctgatctt gtgagagata
    14401 agcattttta gccagttgct gtgtaatcgt tgagccacct ccaaagcggc ccgccgtaac
    14461 tactgccaat aagaagcgtt taagattaat accgctatta ctgtaaaaag tcctatcctc
    14521 agtggcaata acagcattct caagatcatc tgaaatagcg ttgagctcaa cataactccc
    14581 tttttgacca gataaactgc ctgcatactc tcctttgtga tcataaataa cagttgtagc
    14641 cttcaaggca tcttgtaaat cagatacttt agctgttttt gataagtaaa acaagtatga
    14701 tcctaagagt aagacaagag ttccaatcag aatcattagc agttttccga tgtgatagcg
    14761 gcgccaaaaa cgccgaattg gatgttgagg agacggcaaa acagcttcca gcttttgcaa
    14821 ccaagcgggc cgacttttag ggcttccttc ataggctcta ctgcgttgca gagctattgc
    14881 agggtcttgt tctgagtttt tggtagctga tgagtccaaa tgattggcat ttggcgaata
    14941 ctgcttttga ttgtgttgtg gcgttaaacg cgttttttga tgcattaaga attgtttttc
    15001 ctgatatgct ttaggaaaaa atttcttttg taaaagttct agaaatctca taacgttatt
    15061 ataccactat gatcttaaag taaccttgat taggtcatcg aaatatgttt tgaataagcc
    15121 acaccatcca cttgaaagag tagtccttgt ggtccaccgt gatgtgcaaa ttccgtttga
    15181 atcgattttc gagcaggata tctgccatta aagcgctctt taatcatttt ttccatcaca
    15241 ggtatgttcc aaacgtctcg aaatagacaa tccatttgaa caactgcatc taatgtgagc
    15301 cccaccaatg ctaatcggcg ctccatctcg tcaaaggctc cattaatctg actttcaatg
    15361 tcttgtccga cattgccgac acaatagttt aaataataga agtctcctgc ttctacaagg
    15421 cctgtatgcc ctctatcttc gttaacatca taacgtctaa ttgttttcat tgtttttcct
    15481 ctattttacg attatgtttt ta
//
LOCUS       DAUELZ010000041        14110 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.041, whole genome shotgun sequence.
ACCESSION   DAUELZ010000041 DAUELZ010000000
VERSION     DAUELZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.041"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..386
                     /locus_tag="VHI09_000959"
     CDS             <1..386
                     /locus_tag="VHI09_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEQ6120843.1"
                     /translation="NQHELLFGACQSDVKLTNTQEHILMLLSQQRLTNTDLAKALNIS
                     QAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAKPIASEHTHHHDETLNVYNRL
                     LQKFSAKELEIVDKFVTVFAEELEG"
     gene            390..1109
                     /locus_tag="VHI09_000960"
     CDS             390..1109
                     /locus_tag="VHI09_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120844.1"
                     /translation="MRYISVENLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEVETDDEKGGHGHA"
     gene            1102..1917
                     /locus_tag="VHI09_000961"
     CDS             1102..1917
                     /locus_tag="VHI09_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ6120845.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVLGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(1957..2340)
                     /locus_tag="VHI09_000962"
     CDS             complement(1957..2340)
                     /locus_tag="VHI09_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ6120846.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLINLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWEGITINQENWDLTPFLKHL"
     gene            complement(2391..3647)
                     /locus_tag="VHI09_000963"
     CDS             complement(2391..3647)
                     /locus_tag="VHI09_000963"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEQ6120847.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSIDNHNIVEILVAAKISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            3739..6051
                     /locus_tag="VHI09_000964"
     CDS             3739..6051
                     /locus_tag="VHI09_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ6120848.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQEHKGIVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDDTKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGDLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            6315..9881
                     /gene="rpoB"
                     /locus_tag="VHI09_000965"
     CDS             6315..9881
                     /gene="rpoB"
                     /locus_tag="VHI09_000965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ6120849.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            9972..13613
                     /gene="rpoC"
                     /locus_tag="VHI09_000966"
     CDS             9972..13613
                     /gene="rpoC"
                     /locus_tag="VHI09_000966"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEQ6120850.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDSQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDGLEINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAVFTAEAE"
     gene            13765..>14110
                     /locus_tag="VHI09_000967"
     CDS             13765..>14110
                     /locus_tag="VHI09_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEQ6120851.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAY"
BASE COUNT         4114 a         2725 c         3119 g         4152 t
ORIGIN      
        1 aaaatcagca tgagttatta tttggagctt gtcaaagtga cgttaagctt actaatacgc
       61 aagaacatat tttaatgtta ctatctcagc aacgtctcac taatacagat ttggctaagg
      121 cattaaatat tagtcaggcg gcagtaacta aggctatcaa gagtttggtc aaacaagaca
      181 tgctagcagg aactaaggat acggttgatg ctagggtgac ttattttgaa ttaaccgagt
      241 tagctaagcc tattgcatca gaacataccc atcatcatga tgaaacctta aatgtttaca
      301 accgtttatt acaaaaattc tccgcgaaag aattagagat tgtagataag tttgtaacag
      361 tttttgctga ggaattagaa gggtaatcca tgagatatat atcagtggaa aatctctcct
      421 ttcaatatga aagtgagcca gttttagaag ggatcactta tcatttagat agtggagaat
      481 ttgtcaccat gaccggtgaa aatggtgctg caaagtcaac cttaataaaa gcaaccttag
      541 ggattttaca accaaaggct ggacgagtta ctattgctaa aaaaaataaa gacggtaaac
      601 aattaagaat tgcttacttg ccgcagcaag tagctagctt taacgctggt tttccatcca
      661 ccgtttacga gtttgtcaaa tcaggtcgct acccacgtag tggttggttt agacatttga
      721 acaaacacga tgaagagcat gtgcaagcaa gcttagaagc agtcggcatg tgggaaaacc
      781 gtcataagag aattggtagt ttatcaggtg gtcaaaaaca acgtgtggtt attgcccgta
      841 tgtttgcttc tgaccctgat atttttgtgc tagacgagcc aacaacggga atggatagcg
      901 gtactactga taccttttat gaactgatgc accacagtgc acatcaacat gggaaatccg
      961 ttctgatgat tacccatgac ccagaagaag tgaaggctta tgctgatcgg aacattcatt
     1021 tagtcagaaa ccaaaaactt ccttggcgtt gtttcaacat tcatgaagtt gaaacagatg
     1081 acgaaaaagg aggtcatggt catgcttgat attttatcct atgatttcat gcaacgggcg
     1141 gtaatggcgg tagttgccat tagtattttt gctccgattt taggtatttt ccttatttta
     1201 cgtcgtcaaa gtttgatgag cgataccctt agtcatgttt ctttggctgg ggtagcgctt
     1261 ggggtagtcc ttggtatttc accaaccatc actactatta ttgttgtggt tttagctgct
     1321 attttgttag aatacctgcg tgtagtttac aaacactaca tggagatttc aacggcgatt
     1381 ttgatgtcac ttggcttggc cctatctctg attattatga gtaagtcgca tagttcatca
     1441 agcatgagtt tagaacaata cctttttgga tcgatcatca cgattagtat ggaacaagtt
     1501 gtcgccttgt ttgctattgc tgcgattatt ttaatcttga ccgttctctt cattagaccg
     1561 atgtacattc tgacctttga tgaagatact gcttttgtag atggtttgcc cgttcgcttg
     1621 atgtctgttc tattcaatat cgtcactggg gttgctattg ctttaaccat tccagcagca
     1681 ggagcacttt tggtttctac cattatggtc ttgccagcaa gtatcgcaat gagattgggt
     1741 aaaaacttta aaacagttat cttactggga attgtcatcg gttttagcgg tatgttatct
     1801 ggtattttct tatcttattt ctttgaaacg ccagctagtg ccactattac catgattttc
     1861 attagtattt tcctcttagt tagtctaggt ggaatgctta aaaaacggtt attttaataa
     1921 gaaaaatcct cacatgttta atatgtgagg atttatttat aaatgcttta aaaagggggt
     1981 aaggtcccaa ttttcttgat taatggtgat gccctcccaa aagctatcat tagttaaacg
     2041 aggaatcaaa tgaaggtgaa aatgagtccc ctcatccatc aactccttgt cattgctgac
     2101 gctagtgacg cctgaaatgg gaaggtgctg ctctagctta gcaactagag cggcttgtaa
     2161 gttgatgaga tcatagcgca cttcctttgg tagttgtgtc agactagtgt aatggtcttt
     2221 ggaaatcagt aaaaggtgcc ccttttggat aggatcaata tcaaaaacaa ccttgaaata
     2281 ttgactttga gtcagtagct gatttgcttt gagctgatgg caaaagatac attctgtcat
     2341 aggtacatct ccttatgaaa aaacgagcca gagctcgttt aatcggttat ctagtaagtg
     2401 agaacagcgt attttttctt accgcggcga ataacggtta attgatcatc aattttatca
     2461 tcattactta attggtaatc taaatcttga acgcggtctc cgttaatgta gattgctcca
     2521 ttttgcacgt cttcacgcgc ctggcgtttt gatggggaaa tcttagcagc tactaaaatc
     2581 tcgacaatat tatgattgtc tattgattga acgtgataat ttggtacatt gctcaatcct
     2641 tgttttaatt cattagcaga aagatttttg atgtttccag caaataactg ctcggtaatg
     2701 ttaagagctt gtttataagc ttcttcgcca tgaaccaagg taaccacttc gcgtgccaag
     2761 gttttttgag cgagacgctc gtgacgagcg gcattaaatt gagtttcaat ttctgcaatc
     2821 tcatctagag ataagaaggt gaagattttc aagaaacgca ctgcatcatc atccatgaca
     2881 tttaaccaga attggtacat ctcgtaagga gacgtcttat cggcatcaag ccagacggca
     2941 ttaccttctg atttaccgaa ttttttcccg gttgagtcag tgatgagtgg tacagtcatg
     3001 acgtgcccag ttttatcagc ctttttgcgg agcaattcag taccagctgt catattaccc
     3061 cactggtcag agccgccaat ttgtaaggtt acattatgct tgtcattgag ttcgtagaag
     3121 tcgtagccct gcatgatttg gtaagcaaac tcagtgtaag aaatgcctgt ttcaatgcgt
     3181 tttttaacag agtctttact catcatgtag ttaacggtga agtatttacc gacatcacgg
     3241 aggaagtcaa taaagctgat ttgcgagaac cagtcgtagt tgttgacaag ctctgcttta
     3301 ttatcaccat tttcaaaatc aaggaaagta gacaactgcc ctttaatctt gtcactccac
     3361 tccaaaactg tttctttggt ttgaaggctg cgctctgcat ctttaaagga aggatcacca
     3421 attaaacctg tggcaccacc cacaagagcg taaggtttgt gccctgctag ttgcaagcga
     3481 cgagatgtta agatagccac tagatggccc agatgaaggc tgtcagcggt tggatcataa
     3541 ccggtataat aggatacttg cccttctgtt aatgctttga caagggcttg ttcatcagtc
     3601 gtttgaaaga ccaagccacg agctttgagt tcttcaaaaa tattcatagg ctttctccat
     3661 tttcattata gtttcgaatc attatatcaa aaaacgctac tgctatgcaa gctttatcaa
     3721 agtttggtat aatgataaat gaggtatttt atggtgaaat ggaacacgaa acaaaagcgt
     3781 ataagtcatc aaagattagg tctcttggat ttggggccgg tgctattacg tacgttgaga
     3841 ctactgtcta acttttttta tattgttatc ttcctttttg gaatgatggg atttggtatg
     3901 gcatttgggt atttggctag tcagattgaa tctgttaagg taccaagtaa agaaagttta
     3961 gtcaaacaag ttgaatcatt aacgatgatt tcgcaaatga actattctga taatagttta
     4021 atttctactt tagatacgga tttacttcga acaccagtag ctaatgatgc gatttcagag
     4081 aatatcaaaa aagctattgt atcaacagaa gacgaacatt ttcaagaaca taaaggtatc
     4141 gtgccaaaag ctgtttttcg ggcaacattg gcttctgtat tgggatttgg agaagctagt
     4201 ggaggttcga ccttaacaca gcaattagtc aagcaacaag ttttgggaga cgatcccaca
     4261 tttaagcgca agtctaagga gatcgtttac gctcttgcct tagagcgtta tatgtccaaa
     4321 gacaatatct tatgtgatta tcttaatgtt tcaccttttg gccgtaacaa caagggtcaa
     4381 aatattgctg gtgttgaaga agctgcgcgt ggcatttttg gagtttctgc caaagattta
     4441 acggtgccac aggcagcatt tttggcgggg cttccgcaga gtcctattgt ttactctcct
     4501 tatttgtcaa cgggacaact gaagtcagaa aaggacatgg cttatggcat caagcgtcag
     4561 caaaatgttc tctataacat gtaccgtaca ggtgttctgt ctaaaaaaga atacgaggac
     4621 tataaggctt atccgattca aaaggatttt attcaaccgg gaagcgcaat agtaaataat
     4681 cacgattacc tttattacac ggtgttagca gatgctaaaa aagccatgta tagctatttg
     4741 attaagcgag ataaggtgtc tagtcgtgac ttgaaaaatg acgatactaa ggctgcttat
     4801 gaagagagag ccttaacaga attgcaacag ggtggctata ccatcaccac aaccattaat
     4861 aagcctattt acaatgcgat gcagacagcg gcagctcagt ttggtgacct gttagatgat
     4921 ggcactggta cagttcaaat gggaaatgtc ttgacagaca atgcgactgg tgctgtgtta
     4981 ggttttgttg gtggtagaga ttatgctctg aatcaaaata atcatgcttt caatacagtt
     5041 agatcgccag gttctagcat taaaccgata atcgcttatg gtcctgctat tgatcaaggt
     5101 ttaatgggga gtgctagcgt tttgtctaat tacccaacaa cttactcgag tggccaaaaa
     5161 atcatgcatg ctgatagtga aggaacagcc atgatgccac ttcaagaggc cctaaatact
     5221 tcttggaaca tcccagcttt ttggacacag aaattactgc gtgaaaaagg ggtcgatgtc
     5281 gaaaattata tgacaaaaat gggttataag attgcagact actcgattga aagtttacct
     5341 ctagggggcg gtattgaagt ctcggttgct caacaaacca atgcttacca aatgctttca
     5401 aacaatggct tatatcaaaa gcaatatatt gtagataaga ttactgctag cgatggtaca
     5461 gtcgtttaca aacatgaaaa taagccaatt cgtatttttt ctgcagcaac agctacgatt
     5521 ttacaagaat tgttgagagg tccgattact tcaggcgcta cgactacttt caagaaccgt
     5581 ttggcggcta ttaatccgtg gcttgctaat gctgattgga ttggtaagac cggaacaact
     5641 gagaattata cggatgtttg gctagtcctg tctactccaa aagttacctt aggcggttgg
     5701 gcaggacatg atgacaatac ctcattagcg ccattaacag gatataacaa taattctaat
     5761 taccttgcct atttagctaa tgccattaat caggccgatc ccaatgttat tggagtaggg
     5821 caacgcttca acttagatcc aggagtcatt aaggcgaatg tcttgaagtc aacaggttta
     5881 caaccaggaa ctgttaatgt caatggacat actttttctg ttggtggaga aatgaccacc
     5941 agtctatggt cccaaaaagg accgggggct atgacttacc gatttgctat tggtggcacg
     6001 gatgccgatt atcaaaaagc ctgggggaac tttgggttca gaaaaaatta ggttgccatt
     6061 tatcgataaa aagggtataa tagcaataac tatttgtcgt ccaatctagc tgaaatattg
     6121 tccagttagg aagaacagca gttaaatcac actgataaag tcagatttag ctgctctttt
     6181 tgtgtctatt tttagaaaag atagggtttg taacctatat ttaaatattc taaaaattaa
     6241 catttagggc aaatgataac ttagttgcga tttgctgaaa cagcaaagct taaatagaag
     6301 aaggagctaa aaacttggca ggacatgaag ttcgatacgg aaaacaccgt acacgtcgta
     6361 gcttttcaag aatcaaagaa gttcttgatt taccaaattt gattgaaatt caaactgact
     6421 cattccaaga tttcctcgat tcaggtttga aagaagtatt tgaagatgta cttcctattt
     6481 caaactttac ggatactatg gaacttgaat ttgttggtta cgaatttaaa gaacctaaat
     6541 atacccttga agaagctcgt atccacgatg caagttattc tgcaccaatc tttgttacct
     6601 tccgtttggt caataaagaa actggtgaga ttaaaactca agaagtcttc tttggtgatt
     6661 tcccaatcat gacggaaatg ggtaccttta ttatcaatgg tggtgaacgt atcatcgttt
     6721 ctcagttggt gcgttctcca ggtgtttatt tcaacgataa agttgataaa aacggtaaag
     6781 ttggttacgg atcaacagtg attcctaacc gtggggcttg gttggaatta gagactgact
     6841 caaaagacat tgcctacact cgtattgacc gtacccgtaa gattccattc acaaccttgg
     6901 ttcgtgccct tggtttctca ggtgatgatg aaattgttga tatctttggt gaaagcgacc
     6961 ttgttcgtaa taccattgaa aaagacattc acaaaaatcc aagtgattct cgtacagacg
     7021 aagcccttaa agaaatttac gagcgtcttc gtccaggtga gccaaaaact gcagatagct
     7081 ctcgtagtct tttgattgcg cgtttctttg atgcacgtcg ttacgatttg gctgcggttg
     7141 gtcgttacaa agtgaacaaa aaacttaata tcaagactcg tcttttgaac caaatcattg
     7201 ctgaaaacct tgtggatgct gaaactggcg aaattttggt agaagctgga actgagatga
     7261 ctcgtagcgt catcgaatcc atcgaagaac accttgacgg tgatttgaac aagtttgttt
     7321 acacaccaaa tgattacgct gttgttactg aaccagttgt tcttcaaaaa ttcaaggttg
     7381 tgtcaccaat tgatcctgac cgcgtggtaa ctattgttgg taatgccaat ccagatgaca
     7441 aggttcgtgc attgacacct gctgatattt tggcagaaat gtcttacttc ttgaaccttg
     7501 ctgaaggtct tggaaaagtt gacgatattg accacttggg taaccgtcgt atccgtgccg
     7561 ttggtgaatt gcttgccaac caattccgca tcggtcttgc tcgtatggag cgtaacgtgc
     7621 gtgagcgtat gtctgttcaa gacaacgatg tgttaacacc acaacaaatc atcaatatcc
     7681 gtcctgtcac agcagctgtc aaagaattct tcggttcgtc tcagttgtca cagttcatgg
     7741 accaacacaa cccattgtca gagttgtctc acaaacgtcg tttatctgcc ttaggacctg
     7801 gtggtttgac acgtgaccgt gctggttatg aggttcgtga cgtgcattac acgcactatg
     7861 gccgtatgtg tccgattgaa acacctgaag gaccaaacat tggattgatt aacaacttgt
     7921 cttcatttgg acatcttaat aaatatggtt tcattcaaac accttaccgt aaggttgacc
     7981 gtgcgactgg tagggtaact aacgagattg tttggttgac tgccgacgaa gaagacgaat
     8041 acacagttgc acaggccaat tcgaaactaa acgaagatgg cacttttgct gaagaaatcg
     8101 ttatgggtcg tcaccaaggt aataaccaag aattttctgc aagtgttgtt gatttcgttg
     8161 atgtttcccc taaacaggta gttgctgttg cgacggcatg tattcctttc ttggaaaacg
     8221 atgactccaa ccgtgccctc atgggtgcca acatgcaacg tcaggctgtg ccattgattg
     8281 atccaaaagc accatatgtt ggtactggta tggaatatca agctgcccat gactcaggcg
     8341 ctgcggtgat tgctcagcac aatggtaaag ttgtcttttc tgatgctgaa aaagtggaaa
     8401 tccgtcgtca agatggctca cttgatgttt accacattac caaattccgt cgttcaaact
     8461 caggaacagc ctataaccaa cgcacccttg ttaaagtagg agacatcgtt gaaaaaggtg
     8521 acttcatcgc tgatggacca tctatggaaa acggtgaaat ggctcttgga caaaacccag
     8581 tcgttgctta catgacttgg gaaggttata actttgagga tgccgttatc atgagtgagc
     8641 gccttgtgaa agaagatgtc tacacatctg ttcacttgga agaattcgaa tctgaaacgc
     8701 gtgatacaaa acttggccct gaagaaatca ctcgggaaat cccaaatgtt ggtgaagaag
     8761 ccctcaaaga ccttgacgaa atgggcatta tccgtatcgg tgctgaggtt aaagaaggcg
     8821 acatcttagt aggtaaagtc acacctaaag gtgaaaaaga cctttctgct gaagaacgtt
     8881 tacttcacgc catctttggg gataaatcgc gtgaagtgcg tgatacctca cttcgtgtcc
     8941 ctcatggtgg tgatggtatt gttcgtgatg tgaaaatctt tacacgcgct aacggcgatg
     9001 aattgcaatc aggtgttaat atgcttgtgc gtgtttacat cgctcaaaaa cgtaaaatca
     9061 aggtcggaga taaaatggcc ggtcgtcacg gaaacaaggg tgtcgtttca cgtattgtac
     9121 cagttgaaga catgccatac cttccagacg gaacaccagt tgacatcatg ttgaaccctc
     9181 tcggggtgcc atcacggatg aatattggtc aggttatgga acttcacctt ggtatggctg
     9241 ctcgtaatct tggcattcac attgcaacac ctgtctttga cggggcttca tcagaagacc
     9301 tttgggacac tgttcgtgaa gctggcatgg atagcgatgc taagacggtc ctttatgatg
     9361 gtcgcaccgg tgaaccattt gacaaccgtg tatccgttgg tgtcatgtat atgatcaaac
     9421 ttcaccacat ggttgatgat aaacttcatg cccgttctgt aggaccatac tcacttgtta
     9481 cccaacagcc acttggtggt aaagctcaat ttggtggaca acgttttggt gagatggagg
     9541 tttgggccct tgaagcttat ggtgcatcaa atgttcttca agaaatcttg acctacaagt
     9601 cagatgacgt cacaggacgt ttgaaagcct atgaagcgat tactaaaggt aaaccaattc
     9661 caaaaccagg tgtaccagaa tccttccgtg ttcttgtaaa agaattgcaa tcgcttggtc
     9721 ttgatatgcg tgtgcttgac gaggatgata atgaagtgga acttcgtgat cttgatgaag
     9781 gtgaagacga tgatattatg catgttgacg atctcgagaa ggcacgtgaa aaacaagctc
     9841 aagaaaccca agaagtttct gaaacaactg acgaaaaata agcaatcaat tcttattaaa
     9901 taattattta ctggtctggg gcaaaggccc caggaactgg taaagtcatc aaaggcagaa
     9961 aggtaaaact agtggttgac gtaaatcgtt ttaaaagtat gcaaatcaca ttagcctcac
    10021 caagtaaggt ccgttcatgg tcttatggtg aagttaaaaa acctgaaaca atcaactacc
    10081 gtacattaaa accagaacgt gaaggactct ttgacgaagt catctttggt ccaacaaaag
    10141 actgggaatg tgcgtgtggt aagtacaaac gtatccgtta taaagggatc gtttgtgacc
    10201 gctgtggggt tgaggtaact cgtgctaaag tacgtcgtga acgtatgggt cacattgagt
    10261 taaaagctcc tgtatcacat atctggtatt tcaaagggat tccatctcgt atgggattga
    10321 ctcttgatat gagtcctcgt gcccttgaag aagtgatcta ttttgcggct tatgtggtca
    10381 ttgatccaaa agatacaccg cttgaaccaa aatcattatt aacagaacgt gaataccgtg
    10441 aaaaactaca agagtatggt catgggtcat ttgtcgctaa gatgggtgct gaagccattc
    10501 aagatctctt gaaacgtgtg gacttggcag ctgaaatcgc tgaattaaaa gaagagttga
    10561 aatctgcttc tggtcaaaaa cggattaaag cagttcgtcg tttagacgtc cttgatgcct
    10621 ttaacaaatc tggaaataaa ccagaatgga tggttcttaa catcctgccg gttattccac
    10681 cagatcttcg tccgatggtt caattggatg gtggtcgttt tgcggcatca gacttgaatg
    10741 acttgtaccg tcgtgtgatt aaccgtaaca accgtttggc acgtttgtta gaacttaatg
    10801 cccctggcat cattgttcaa aatgaaaaac gaatgcttca agaagccgtt gatgctttga
    10861 ttgataatgg tcgtcgtggt cgtccaatca ctggaccagg aagtcgtcca ttgaaatcat
    10921 tgagccacat gcttaaaggt aaacaagggc gtttccgtca aaacttactt ggtaaacgag
    10981 tagacttctc tggacgttcc gttatcgctg ttggtccaac ccttaaaatg tatcaatgtg
    11041 gtgtcccacg tgaaatggct atcgagcttt ttaaaccatt tgtaatgcgc gaaattgttg
    11101 ccaaagaata tgctggtaac gttaaagccg ctaaacgtat ggttgaacgt ggcgacgaac
    11161 gcatctggga tatcttagaa gaagttatca aagaacaccc agtcctactt aaccgcgcac
    11221 cgacccttca cagacttggt attcaggctt ttgaaccggt tcttattgac ggtaaggcac
    11281 ttcgtcttca cccacttgtg tgtgaggcct acaatgccga cttcgatgga gaccaaatgg
    11341 ccatccacgt gccactttca gaagaagcac aagctgaagc tcgtcttttg atgcttgctg
    11401 cagagcacat cttgaaccct aaagatggta aaccagttgt taccccatct caggatatgg
    11461 ttcttggtaa ctactatctt acgatggaag atgctggtcg cgaaggcgaa ggtatgattt
    11521 tcaaggataa agacgaagct gtgatggcat atcgtaatgg ctatgctcat cttcatagtc
    11581 gtgtgggtat cgctgttgac agcatgccaa acaaaccttg gaaagacagt caaagacata
    11641 aaatcatggt gacaactgtt ggtaagattc tctttaacga catcatgcca gaggaccttc
    11701 cttacctcca agagccaaac aatgccaact tgacagaagg aacacctgat aaatacttcc
    11761 ttgaacctgg tcaagatatc caagaagtga ttgacggctt agaaatcaat gtgccattca
    11821 agaagaaaaa cctcggtaac atcattgcgg aaaccttcaa acgtttccgt acaacagaaa
    11881 catcagcctt ccttgaccgt ttgaaagacc ttggttacta ccattcaacc cttgctggtt
    11941 tgacagtggg tatcgctgac attcctgtta ttgataataa agctgaaatc attgatgctg
    12001 ctcaccatcg tgttgaagaa attaacaaag ccttccgtcg tggtttgatg acagatgatg
    12061 accgttatgt tgccgttaca acaacatggc gtgaagctaa agaagccctt gaaaaacgtc
    12121 tgattgaaac acaagatcct aagaacccaa tcgttatgat gatggactca ggagctcgtg
    12181 gtaacatctc taacttctca cagcttgcgg gtatgcgtgg tttgatggct gctcctaacg
    12241 gacgcatcat ggaacttcct atcttgtcaa acttccgtga aggtttgagc gttttggaaa
    12301 tgttcttctc aacccacggt gcacgtaaag ggatgaccga tacggccctt aaaacagccg
    12361 actcaggtta tcttactcgt cgtttggttg acgttgccca agatgttatc attcgtgagg
    12421 acgattgtgg cactgatcgt ggtcttctta tccgtgctat tacagatggt aaagaagtta
    12481 ccgaaacgct tgaagagcgt cttcaaggtc gttacacacg taaatcagtc aaacaccctg
    12541 aaactggtga agtcttgatt ggtgctgacc aattaatcac tgaagacatg gctcgtaaga
    12601 ttgttgatgc gggtgttgaa gaagtgacca ttcgttctgt ctttacctgt gcgactcgtc
    12661 atggtgtctg ccgtcactgt tatggtatca accttgcaac tggtgatgct gttgaagtgg
    12721 gtgaagcagt tggtactatt gctgcccaat ctatcggtga gcctggtact cagcttacca
    12781 tgcgtacctt ccacacgggt ggtgtagcct caaataccga tatcacacag ggtcttcctc
    12841 gtattcaaga gatctttgaa gcacgtaatc ctaaagggga agcggtcatt actgaagtga
    12901 aagggaatgt cgtcgagatt gaagaagatg cgtcaactcg taccaagaaa gtctacgttc
    12961 aaggtaaaac tggcatgggc gaatatgtcg taccatttac agcacgtatg aaagttgaag
    13021 ttggcgacga agttaatcgc ggagctgctc ttacagaagg gtcaattcaa ccgaaacgtc
    13081 tccttgaagt gcgtgatacc ttgtcagttg aaacgtacct tcttgcagaa gtacaaaaag
    13141 tttaccgtag ccaaggggta gaaatcggag acaaacacgt tgaggtaatg gttcgccaaa
    13201 tgcttcgtaa agttcgtgtc atggatccag gtgatacaga cctccttcca ggtacactta
    13261 tggatatttc tgatttcaca gatgctaaca aagatattgt tatctctggt ggtatccctg
    13321 cgacatctcg tccagttctt atgggtatta ctaaggcttc ccttgaaacc aattccttct
    13381 tatcagctgc atccttccaa gaaacaactc gtgttcttac agatgctgct atccgtggta
    13441 agaaagacca cttacttggt cttaaagaaa atgttatcat tggtaaaatc attccagctg
    13501 gtactggtat ggcgcgctac cgtaacattg aaccacaagc gatgaatgag attgaagtga
    13561 ttgatcatac agaagtctca gcagaagccg tttttacagc agaagcagaa taatctcatg
    13621 aaagagtcct taggggctct tttttgtctg agtttgttgg cagtaaaaag cattgacaat
    13681 cagtcttagt cccaatgagc agatgttttt caaaatactt atgatttagc tataataatg
    13741 cataagagga ataggtaatg tgttatgtat caagtaatta aaatgtatgg tgattgggag
    13801 ccttggtggt tcatcgatgg atggcaagac gatattatag atgagcaaca atttagtgac
    13861 tggcaagaag cgcttgatta ttttaatcaa gaatggcagc gcatgaaagc tatttttcct
    13921 agttatcata gtcaaaagaa tttgttagct actttttggg aaaaagaaga taaaagatgg
    13981 tgcgaggact gtgacgaaga tttacagcag tttcattctc ttttactcct taaaaataaa
    14041 gatattgtac caagcaataa ttatatccct gaatttgaac aacgaaatga ttcaccacag
    14101 gtagcttatc
//
LOCUS       DAUELZ010000042        13890 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.042, whole genome shotgun sequence.
ACCESSION   DAUELZ010000042 DAUELZ010000000
VERSION     DAUELZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.042"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(30..2045)
                     /gene="recG"
                     /locus_tag="VHI09_000968"
     CDS             complement(30..2045)
                     /gene="recG"
                     /locus_tag="VHI09_000968"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEQ6120852.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENRSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSVIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     HKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAKNIRKKEVYD"
     gene            complement(2420..3874)
                     /locus_tag="VHI09_000969"
     CDS             complement(2420..3874)
                     /locus_tag="VHI09_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120853.1"
                     /translation="MERLGGADLDYCYHNHHDTSMISAEQLVFTYHDQKNPACQVSTC
                     QIASGQFIVLCGSSGSGKSTFLKFLNGIIPDYYAGKYEGRLDVADCQAGRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQDLLALTDEQRQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            complement(3811..4491)
                     /locus_tag="VHI09_000970"
     CDS             complement(3811..4491)
                     /locus_tag="VHI09_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ6120854.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KRYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            complement(4488..5081)
                     /locus_tag="VHI09_000971"
     CDS             complement(4488..5081)
                     /locus_tag="VHI09_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEQ6120855.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPIVLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSAFAHYLLKKHFIKSGLLS"
     gene            complement(5078..6748)
                     /locus_tag="VHI09_000972"
     CDS             complement(5078..6748)
                     /locus_tag="VHI09_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120856.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     EACGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGSLKEVNKK"
     gene            complement(6741..8504)
                     /locus_tag="VHI09_000973"
     CDS             complement(6741..8504)
                     /locus_tag="VHI09_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEQ6120857.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHTIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLPLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQAIERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLSQETINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLNTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            complement(8501..9337)
                     /locus_tag="VHI09_000974"
     CDS             complement(8501..9337)
                     /locus_tag="VHI09_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120858.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATLSLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDIQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNDIRTQRRDRNK
                     NR"
     gene            complement(9334..10356)
                     /locus_tag="VHI09_000975"
     CDS             complement(9334..10356)
                     /locus_tag="VHI09_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ6120859.1"
                     /translation="MTASDLQRDQSLLTKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYEAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            complement(10358..11242)
                     /gene="isdE"
                     /locus_tag="VHI09_000976"
     CDS             complement(10358..11242)
                     /gene="isdE"
                     /locus_tag="VHI09_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEQ6120860.1"
                     /translation="MIKRCKGIGLALMAFFLVACVNQHPKTAKETEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSANPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHS"
     gene            complement(11226..12101)
                     /locus_tag="VHI09_000977"
     CDS             complement(11226..12101)
                     /locus_tag="VHI09_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEQ6120861.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTGVVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTALDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDNQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            complement(12298..>13890)
                     /gene="shr"
                     /locus_tag="VHI09_000978"
     CDS             complement(12298..>13890)
                     /gene="shr"
                     /locus_tag="VHI09_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEQ6120862.1"
                     /translation="EISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDV
                     KFNQIKSVSPKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTN
                     SAIDKELVSIEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKASNGQYVTVNEKLL
                     SNDPKDDLTGEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDL
                     VEKGLRALNQKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEF
                     RGIKVGKMLGYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKN
                     YPKVLRMKLIEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVK
                     ETNNPQENSHLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKATGQSTQET
                     SKTDDTDKAEKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLI
                     AGLSSFMIVALGFIIGRKTLFK"
BASE COUNT         4438 a         2882 c         2352 g         4218 t
ORIGIN      
        1 atatttgtaa aacaacgact tgttttaagt taatcataaa cttctttttt tctaatattt
       61 tttgctacta attgccactg tttttcatat atccagttag gatcagaaac aatcgctgca
      121 gaaactttgc gtgcttcttc taaaatagga taatcctcaa cgatatcagc tacttgaaat
      181 tctggaattc cagactgacg agtaccaaag atttcaccag aaccacgcat ttttaaatcc
      241 gactcagcca aaacgaaacc atctgtcgtt tctgtcatga ttgtcattcg ttttttcccc
      301 gaatccgttt tgggattagc cactaaaaca gcgtatgatt gtttatgtcc acgaccaaca
      361 cgcccacgaa gttgatgtaa ctggcttaat ccaaaacgat cggcatccat aataatcatg
      421 atcgttgcat ttgggacatt tacccctact tcaataactg ttgtggatac taaaatatga
      481 ctttttttat ccttgaaatc ttgcattatg gcatcttttt catcattttt catacgtcca
      541 tgtacaagag caaccttagc aattccttca aaataagtag ataattcagc atgcaatgct
      601 actgcattct ttaaatctaa agcttctgat tcttcaatca acggtgaaat gacatacact
      661 tgagcatctt tttgcaattc accttttacc cattccaaca cagtccctaa ctgctcgtgt
      721 ttcacccagc gtgtcataat aggtttacga ccggcaggta attcatcaat aacagaaaca
      781 tccatttctc caaaagctgt gattgctaga gttcggggaa ttggggtggc tgtcatcatt
      841 aaaacatcag gattttctcc cttttcacgg aaaattctac gctgtttaac accaaaacga
      901 tgttgctcgt ctgtaatgac aagccccagt ttatggtact gtaccgagtc ttggataaga
      961 gcatgagttc ctacaatcat atcaaccgag ccatttgcaa tagctgctaa aaccgtacgc
     1021 ttaacagcgg ctttcatacc cgaagttaat atagcaattg atagatctgg aaataactct
     1081 tgcagactaa tgtagtgttg ttcagctagg atttccgttg gaaccatcaa agccgattga
     1141 aaacctgctg tataagctgc atacattgat agaccagcaa tgactgtctt tcctgatcct
     1201 acatctcctt gcaataaacg attcatatga gctcccgatg acatatcaga taatatgtca
     1261 tctaaagagc gcttttgagc atttgttaga ataaagggta aagaggaaat ctttgtctcc
     1321 atagcatgtt tactataaag aataggcaaa ccatttgttt cagatctatt ttcggctttc
     1381 aaaacttgaa ggttcatttg aaagtaaaat aattcttcaa atttaatccg acggagcgct
     1441 tgcttatact ctgtgatatc ttttgggaaa tgcatagcta aacaagcctg actacgaccc
     1501 atcaatcggt atttttccaa taaagtagct ggtaaatttt ccttcaattc caaatgcgca
     1561 tcgatttcaa aagctgactt aatagcttta atcaaagtag actgtgaaat tccctgagct
     1621 acgcgataaa caggttgcat gtcatcttca acttgagcta aaaccttcat cccagtaata
     1681 gccgatttag tggcatccca tttaccaaaa acagctacct cttgaccaag ttctatttta
     1741 tcagctaaat agggttgatt aaaaaaacta acatttaaga cagcttcccc ttgacgcaat
     1801 ttgaaactta aacggttacg tttaaaacca taatattgta cattagctgg agtaacgact
     1861 aagcctgtaa tgactgcttt ttcaccatcc actaaatcaa aaacagattt tgatttaaaa
     1921 tcttcatagc gaaacggata ataaagcagt aaatcttcta ctgtataaat atctaatttc
     1981 tgaaattttt ctgctgattt tggtccaaat ccctttaagt tggacatagg agctgttaat
     2041 atcattatct ttttcccttc acaaaaactc ttgtcagatt gaagacaaag tttgtcgtga
     2101 tactttcctt aggtagtgct taaggcagcg acgttctttg gaattccatg cctttatttt
     2161 tgagcctgag gtctcaaaaa tcccgagtgg cgtcaatctc gtattgacgc cactctttta
     2221 ccacagcaaa aagcatctgt tttggctcac tttgtgtgta aaaatcggaa accggatgtc
     2281 aaatcagcag agctattcat gctttttgag cataaaactg gggttgtcta cgttctgact
     2341 cttgtatcta ttatacaaga gttcctattt attttcagat aaaagccttt tagtactttt
     2401 aaacagttct aggggctcgt taaggtagca tttgataaat ggtatcgcag acactactta
     2461 gaagttcttc atcatgtgaa atgaggatga caaccttatg ttgggtcttc agctgcatga
     2521 gaagattagc taaagccttc atttgtaaca aatcaagtcc gctacttggc tcatcaaaaa
     2581 ttaagatatc tttatcagcc aaaagactag cggcaatcat cactctttgt tgctcacctc
     2641 ctgacaaact agctggatga cgttctaaaa gagtcgttaa agataaccgc tctattactt
     2701 ctgtcgtccg tggatgtcgt tcatggccca agttgacttc ctttgaaacc gattctgcaa
     2761 atagttgcaa agaaacttct tgtaagacaa tactagtctt tgataaacgt tgcttggcag
     2821 ataaaggtat gccttgaaac ttaatagttg cttttttatc gtctaagata ccagctatat
     2881 aataaactaa ttgcgatttt ccaagcccgt ttgatcctgt aatgccagaa atacttcctg
     2941 cagcaaaact aagctgttca atacatctaa gtattttgct tcccgctcta actgttagat
     3001 gctcaataca caggctatca tctgggcgat aatgttgact ctcaatctta ccagctaaca
     3061 caggttttag gtcagacaag tctaagcgac gtagtcccat atcttgccgt tgttcatctg
     3121 tcaaggctag caaatcttgt gttgttaact tatctgttaa tctaccattt ttaaagtaga
     3181 aaaagttatc tgccaaatcc atcaagtaat gtaagcgatg ctcggctaca ataatggttt
     3241 ttcctgctgc ttttaattgg gtaagatagg ctttaacagc agctatgcct gcactatcta
     3301 agttagcagt tggctcatca aaaagcataa tattagtacc ttgcataatg gccgttgcaa
     3361 tagcaactct ttgtttttgc cctcctgaca atccaaacat atccttgtta aggagctctg
     3421 caaaagcaaa atcttctgct aaagtccaaa gacgcttcat gataaccttt gcatctagac
     3481 cttgattttc acagggaaag accagttcgt gttggacttc gcgatagaaa aactgactgg
     3541 ctggattttg aaaaacagag gccacagatc tagaaaatgt ttcaacactg tctctgcctg
     3601 cttggcaatc agccacatct agtctacctt catattttcc agcataataa tcaggaatta
     3661 tgccattaag aaattttaaa aaggtagatt tacctgaacc agaagatcca cacaaaacaa
     3721 tgaattgtcc agaggcaatt tgacaagtcg atacctgaca agctggattt ttttgatcat
     3781 gataggtaaa aacaagttgt tctgctgaaa tcattgaagt atcgtgatga ttatgataac
     3841 agtaatccaa atctgcaccc cccaatcttt ccatgttaag tgtgagctaa agcattcact
     3901 ggtgcctttg ttgactgcta gtcctttagt tagactagcg atagttagtt cttgactact
     3961 tcttatcaaa gataaaagca atggaaccat taaatactcc aagtatcgtt taggtctgat
     4021 caaaatagac cattttgtta aaataatccc cctgattgtt aaagagcgat gaataacaca
     4081 acattcttgt ctaatcattg gtatgaaacg gcacataacg gctaaggtca atagccagac
     4141 ttctgggaaa tgccatttgc gtaatccatg aaccaattcg tagatggtag ttgtcttaat
     4201 agtaattaaa cctgctagta aagaagggaa aactaacctt cctgctattg agacaaagct
     4261 caataagtga tcaagataag aagggaaaat cgatagcggg attacagata gaccgatcat
     4321 taacagataa acaaccgcca acttgaacgc tagctttttc ttatttaaag cagacaaaag
     4381 taataagaga aaaataacaa ttataaattc taaaaagcca tcaacacgaa aaaagaagca
     4441 ggcattggca agtaccaaca aaagcagttt ggtgcgaaca tcaagcctca tgataacaat
     4501 cctgatttga taaaatgttt ctttagtaaa taatgtgcaa aagctgaccc taataaggct
     4561 cctgcaagaa caattcctac aaaccaaata acatgcccta aactaaaatc atacataatg
     4621 cgattgacat aggtcatact tttaccgcgg gctaacaaac tttcaacata ggcatctttc
     4681 aacaaccaca tcagcacaat agggccacta ttgacaaaag caaaaaccat ataagatagg
     4741 tcattaaaaa accttgactt gtattttcct aaagacgcaa taatatccgc tattaaaccg
     4801 aaaataatgc tgggaaacat cgcggcaaaa aagtgaccac taaggaagaa aaaacctccc
     4861 atcatcaatc ctaataatgt tattggccca aatgtttgca atttagcaat caaaagaaaa
     4921 taaattgttc cacccagtaa agctgcaaag gcaggggcat ataacatatt tcctgagcga
     4981 tcaaaaaaga tgttaataaa cgtgcctaga ccaacacaaa taaagtagag cgcggcaaat
     5041 gcgcctgtta tcataatatc ttttattgtt aactgtttca ttttttattc acctctttta
     5101 gggaaccatt ttttacgcaa aatagatgat cagcacaagc tagggttgat ggcctgtgag
     5161 aaattaagaa gaccaatcct tgacaatgtg ttttaattaa atcaagcaag atagcttcat
     5221 ttagagaatc taaatttgac gtgggttcat caaaaatata gcaactcgca tctttcaata
     5281 aagctcgcat caactctaaa cgttgacctt ctccagctga aaaatcagaa ggactcttga
     5341 taatcgtatc caaaccttcc ttacaagcta acagtctgtc tttcatacca caagcctcag
     5401 ctaaattcca aatggactca tctgaaatat cttgacggcc aaaggtaaga ttttcacgga
     5461 tagactgttg gaaaagctgc gctgtttgtg gcacataagc aatagtatct tgtaatttag
     5521 cagcatttag taagcaacta ttcctatcat taagaaaaat atctcctgtc ttccaattat
     5581 accatttcat aattaatttc accaaagttg atttaccaga tcctgattcg cctttaatgc
     5641 caataatccc cttttcttga aaggtaactg ataaatcctt aaaaaccagc tcttggcgct
     5701 ttggataagc aaaactgagg tcctcaaaag aaacactcct taaacgatct atctgttttg
     5761 tgccttcgtc tactatgaca ttttcgtcta gaaggtcaaa aatatttcga gccgcattca
     5821 tagcacgctt aaagcctaat ggcaaacgtc ctagttctaa aaaaggagaa aacgaagcag
     5881 tgaaagcaat aaaagttaat aagcctttat caaatgataa gctgtcatgg catatcccat
     5941 caaaaaccat ataagaaaat gctaagatca ttaagccaag ccaaaaaaaa gtcaatgcca
     6001 tttgcaaaaa ttgggcttgc gctacgttac gatcaagagc atttacatga ttactttttt
     6061 ttattaatcg ttcaaattgc tcgtctaata cctgaaattg caaaagatct ttgactgatc
     6121 gtaaactctc taaaaaataa gacaggtaat cttttcgacc ttcattttgg ctttttagta
     6181 aaacctgtaa aatattagca aaataaatgg gtataacaat ggctaaacaa gcataggttg
     6241 ccaaagctaa taaggctaac tgccaactac tttgacaaaa gtaccagaac attaaacctg
     6301 cagacaatat tgctgtacaa ataggtgcga ttgtgtgggc aaaaaaaact tctaatgcct
     6361 caatatcttc tccaatcatt ttaagcaaat aaccactgtc ttgactatcc aagcaagctg
     6421 gagaaagagc tcttagtttc ttaaagatca agttcctaaa agctgctaaa ctatgaaaag
     6481 caacaaaatg accaaaataa tgctctccat aacgaacaaa acctcttaaa aaggctagag
     6541 caatcaaaag atataaagct aatattggaa tcacttcttg tctaatagct agtagtccta
     6601 aataagctat tccagttgga atacttaccg tgatgacaaa acccataaca gcaaagccaa
     6661 cagccaagat aatccaaggt agcagagttt tcattagggt taataaacgc caaaccaggc
     6721 ttaaggtaga tctgttaggt ttagccataa atacttgcct ccagtcttgc ttgagtatca
     6781 actaggtgac gataagttgg ttggtcccga taaaggtcgc aaggtttgcc taaacaagca
     6841 ggctgccctg tgtttaaaaa gagtacttgg ttagctcctt tcagtcgaga catcttatga
     6901 gtaatcacaa taactattgc tgtcttagct aatctcgtta ttaaattgtc aataatacgt
     6961 tcattttctg catctagact agatgttgcc tcatcaaaga tataaagaga cctcttactt
     7021 aatagagctc ttgcgcaaat cacctgttgc ttttggcctg gtgataacag gttaccattt
     7081 tcaccaacta ttgtgtcaag accatctggt agccagttga tgaagcttaa caaaccatgt
     7141 tgatctatcc aatctaagat ttctttttta gagagatttg ctgctaacct taaattatcg
     7201 taaatgctac ggttcaataa ggtagattgg tccgatacat aaagaacctg ctggttgata
     7261 gtttcttgcg acaaattgtc actgtctaaa ccgtcaaata aaatatgccc agtcgttgca
     7321 gactggcgtt tcaagaggag ttgagctaga cttgtttttc cttgtcctga cacccccgcg
     7381 atagctgtta gttggccctt cgttaatgtc atggtaaccc cactaagcac tgtttttttc
     7441 tcataagcta aagaaatatt ttggatgtca atctggttaa agtttattgc tgttttactc
     7501 ttgttatctt tccttgctgg aacactatcc aagaaactaa agatacggtc agccattttg
     7561 gtattcatca tgaccaagtg catgccataa ccctgctcac gaatcggtgt aaaaaattct
     7621 gtagcaatca agagaaaaaa gaggaaatta aaaaaggata actggcctgt tgccaaagct
     7681 tgaacggcta aaaaacctga caaaccaatt cccagataca taactgcatc catataacca
     7741 actgcttgta actgaaagcc aagcaaagac atggtagcct ttcgaaactg ttctgcctta
     7801 cccgaaaatt cttgctcgta acgttctatt gcttgataag aatacagtgt attaagacct
     7861 tttaagtcat ccataaataa attaccaaca tccatataag aagaccaata atgactcata
     7921 atttgcttag accgcttttg catagcaacg attgaaagtg gaatcagagg cagaactcct
     7981 aaaaagacaa ggcctgccaa tggataaatt aaaaagacta gtatcataat agttgtgcag
     8041 ttccacttgg ttctcataga caaagacaaa tagtaaccat aataagtatc caaactatca
     8101 atcccctgtg aagccaacgt taaaatatca gcatcactgg catgagcatc aaattgcccg
     8161 tctaaatcaa taaaagcctc aaagaaagat tgtttgagtg aatctctagc aaattgtgaa
     8221 gcgatccctt gcagttgctt gcctaatata gcaagaacaa agcctaccac atttaaaagt
     8281 aggagtaaac ctgccaagcg tcctaaggca atagtatgac ccaaaataaa ggtcgaaaac
     8341 gttttggcaa tgaggtaaaa actgatcatc ctcatcacaa attgtaacca agataaaaag
     8401 gctgacacat aaagcaaata cctttttggt gcaattcgtt cacgtaatcg ttttaacaat
     8461 cttttccttt ttccccttga tattgtatct tctgtttcca tcatctgttc ttgttcctat
     8521 ctctcctttg agttctaata tcattggttt cattgaccaa tgaagtgagg acaaactgtt
     8581 gaccatttct ctcaacaaag ggcaattcaa caccaaagac atctctgata aactgttgat
     8641 caaggaggtc tcttggttgc ccttgggcat aaagtcgccc atctttcagt ccaataagat
     8701 ggtctgaaag agacaaggcc tgattgatgt catgtaatac taacacaatc gttaggccta
     8761 gctcttggtt gattttagta atcagcttga gaatgtcaag ctggtactta atatccaaat
     8821 aagtcgttgg ctcatctaaa aaaataatat ctgtatcctg ggcaagggcc atggcaatcc
     8881 aaaccctctg ctgttcacca ccagaaaggt gttggatgtc acgataaaca taatcactca
     8941 aagccgttac ttctagggcc caagcaaggc gttcctcatc ttctttggta gtagttttaa
     9001 aaaaagaacg acgggcaagc ctagccatgg ctaccaattc acccactaac aaaccttcga
     9061 ccacttgatg gtattggtgt accactgcca cttttttcgc aaaagccttg agagaaagcg
     9121 ttgctaattc ttcctgctca agccaaacct gcccttgttt taacggaagg tttttggtta
     9181 ataattgtaa taaggttgac ttgccactgc catttgcccc cataatagtg gtaattttcc
     9241 cttttggaat gctaaaggtc aggtcatcta aaacaagttt ttcctcataa gcaaaggaaa
     9301 ggttttgcac ctgaatatga ctgctgttct tactcatata atatcagacc tccttagcag
     9361 gtaaataaag taaggcccgc ctacaatact cattataatc gcagggctaa tttccaatgg
     9421 gtaagcaaga cttcttccta aagtatcggc tagcaaaaag acaaaggctc caagcaaagc
     9481 tgaaaaagga attaagattt ggtgcttact tccaaccagc aagcggctca tatgagggac
     9541 aatcaagcct aggaaagaaa ccactccaac gatcgaagtg gctaccgaag ccaataaaac
     9601 agcaaccaag gaaattccca accgtaacgc tgtagcatcg attcctaaat gacgaatcac
     9661 ctgatcatct aaaagaagaa ggttacaagt ttttgacaat aacaaagcta gcaacaagcc
     9721 aatgaatgta taagggaata aaacccctac atcagcccat gttttttgac taatatgacc
     9781 tgctaaaaga gcattgacca tgggactagc tgaggtgaaa aaactggtta aagcagttga
     9841 taaccccata aataaagcat taatagcaat ccctgttaga ataagccgaa taggattaag
     9901 ccctttcttc catgctaaac cataaatcag caggaaagac actccgcctc ctaaaaagga
     9961 tacgattgga ccatacagca gcaactgcgg tagaagtaaa ctagagacta aaaccataaa
    10021 actagcacca ctacaaatcc ctataatagc agggtctgaa ataggatttt tcaacactgc
    10081 ttgaaaaaga actcccgaaa cagctatccc tgctcctgct aataaagcaa tgacaattct
    10141 tggaaagcgc aaatcataaa tcaaagccac ttgggggtca taagccacaa acagtccctt
    10201 gacaattgcc tcataagaaa ccgccaaacc accaagggat aaggcaataa ccgccaaaaa
    10261 caggaggctg agaattaaaa gaactagata aagaccactt ctgaaacgtt ttttagtcag
    10321 tagggattga tccctttgta aatctgatgc tgtcatgtta cgaatgatct cccacgtggt
    10381 caaaaagctg tgttaaggtg tccaaggctt ctgggtagtt caatttagca ctcatgccaa
    10441 acagggtatt gtccaaatca tagactttcc cttccttgac tgccgtaaaa tgtttccaaa
    10501 tatcattttc agcaaattct ttgtcaaaca tcacttttac cttgtctgga atggcgtgag
    10561 ctgttcgtaa aatcaagtca ggctccttag ccagcatgtc ttcaggatta gctgatagaa
    10621 attctttctc atctgactga taaacattct caccacctgc caagtccaaa agattcccta
    10681 cataagattg gttcgtcgcc accaaataac tacctggcaa gcccataaga ataagcactt
    10741 taggcttttt cttcccctta cgtttagctt ggaaagcacg ataatagtcc tggtattgct
    10801 ggcgcaattc ttttgcttct tgttgacgtt ggaaaaggtt ccctaaatca tcaatggact
    10861 ggtacatgcc ctcaacactt cgtaagttca aaaaaccata ctcagtatcc aatttttgat
    10921 acttgggttc caaatcttct tgtaaagaat tgggactcaa aatccaagtt ggtttcaaag
    10981 aagcaatcaa ctctatatca ggattcatgg gtaaacccac acgcttaaca gcatcatagc
    11041 gtttaggaag ggtatataat ttactatcac aaaccccaac gaggtctaaa tttaaacggt
    11101 cacagatatc aaccacagca accgaagtgg ctacaattct ctgctgttca gtctctttag
    11161 ccgttttagg gtgctgattc acacaagcta ccaaaaagaa ggccattaag gctagaccaa
    11221 ttcctttaca tcgttttatc attttttttc ctctttttcc aaacccatac agcaaaccct
    11281 cctaacatca gaaaaaaagt tggaaagtag taaatcatga ttgataagtc ttttttagac
    11341 cgaaacttat cttctttttt taactctaaa gaagtcgtta agcccatctt agaaggaaga
    11401 atttttttat ctgatagact cttgttattt gatgagttgg ctttaggctt attctgagtt
    11461 atcatagcac ctgtgtgaga ctcatcttga ctttctccta gttttgtatc aacaggcttt
    11521 tgattgtctt ttacttcttg attttgagaa ttgtctgttt cagtaacaag ctgggccaac
    11581 atattacctg aatacttttg tattaactct gaagctgaca ggtaaaagac cacttctctg
    11641 cccataggtt caacaaacat gcttcctcga atgatactat ttacagtagg caaactaata
    11701 gcaatatcta gtgctgtccc attggtatca gtaccttttt gggtgatatt gtaatcaact
    11761 gcctgaaagc ttccagttcc tccaggttgt atccagaact gataattgcc cgaataatca
    11821 gctaagctca tacgaaaagt aagaacgatc tttccagcat cagatacttc tagcatggca
    11881 tcactataga cagtcccttc aaccatacct tgaccaatat caaaggaatg ctcacctccg
    11941 ctatcttcta tctgcccaga tatcggatga cgataatttc tctgaataat acatccataa
    12001 atttgacctt tatctgcata cacaacacct gtcattgtcg ataaacttat catgaacact
    12061 actattacct gcaacaattg ctttattact acctttgtca tatgctcctc ttgtctatct
    12121 tttcttaaac atcttatttc attttttaaa tgattcccat tttataacat ctccaaatgt
    12181 gaaaaacaca cctatcttgc gactacttca acctgtatca ggagtcctgt cggaaatagg
    12241 tatgttttta gctcttgctg actagctttt ttgttatttc acttggtact aagtttctta
    12301 tttaaataat gtctttcgac caataatgaa tcccagagct acgatcataa agctagatag
    12361 acctgcaatt aagtgataac gagagctact ttcttcacca tttgattgtt gattggaacg
    12421 atgtttctta tcagattttt tagtcttagt atcttttgcc gttttacctt caattgatgt
    12481 ttgatggtca cgcactaact gtttcaattt ctctgcctta tcagtatcat ctgtcttaga
    12541 agtttcttga gttgattggc cggttgcttt tttagccagg agatcagtta agttagcaat
    12601 accagtagct gctgaaggag gacttgtagg tgttttttct tgttgacgat tttgaggtcc
    12661 tttcaactga tctgttgaag ttagatggct attttcttgt ggattattag tttctttgac
    12721 aacctttgcc ttctctgtcg taaggcttgc ccagtctaaa cgcataaaaa cggtttgaag
    12781 gccagaccct tttgaaatgg catccatgat aggcacaaag acctgtaatg gcacaagtcc
    12841 gtctttcttc gcttgttcaa tcaatttcat acgcagcact tttggatagt tttttccata
    12901 acgatctgcc aatggtaggc cagttacatc cgtgaaataa gacacaacct ctgccttttt
    12961 tgttcgacca actggtttac cagctaaatc tctttggtaa ccatctacga aatagctcaa
    13021 ttcaccaagg tagcctaaca ttttaccaac ctttatccct ctaaactcaa cttctaaata
    13081 ggaaacacct tttttaacta ccaaagtcac tcgatgatta atagccttgt ttgacatgga
    13141 ctcagatgct aagtcagttt ttaacatgct ggcatttaaa tagtaaatac catcacgcaa
    13201 ttgtttttga tttaatgctc taaggccttt ctcaactaaa tctttctgat gtgaatgtgg
    13261 tcctttcgcc acaaggatat cattagatgt caaatagata tgttcttttt gagtagcaaa
    13321 tttctttgtt attaaagctt tgcgaattcg atatgtacct ggatctttta aagaaaactc
    13381 tcccgttaag tcatctttcg gatcatttga gagaagcttt tcgttaaccg tcacatactg
    13441 tccattggaa gctttttttt gcaactgaat cacaatactc caatcgactt gcatatcatt
    13501 aagtaaagaa acagtatctg aacctttttc ttgtaacaat tgttgatatt cttcaataga
    13561 cactagttct ttatcaatgg cagaatttgt tttttgctcc caataataca aatctaaaag
    13621 actgaaagct tgatgatatt ttattttttc gccatctaaa gaagcttcaa gttttgcaat
    13681 atgtttttgt ggagtcagtt tgtgttgtcc gataagagct cttacttttg gactcacact
    13741 ttttatctga ttgaacttca catctaaatt aagcgtttca acatctggaa atactgattc
    13801 ttcaacctga gtaagcaagt tattgaaaaa atcaagtttt gttaattggt tattctgttt
    13861 aaatatacca tttggaagat ttgaaatttc
//
LOCUS       DAUELZ010000043        13868 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.043, whole genome shotgun sequence.
ACCESSION   DAUELZ010000043 DAUELZ010000000
VERSION     DAUELZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.043"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            88..276
                     /locus_tag="VHI09_000979"
     CDS             88..276
                     /locus_tag="VHI09_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEQ6120863.1"
                     /translation="MLTYDEFKQAIDREYITGDTVMIVRKNGQIFDYVLPHEEVRNGE
                     VVTIERISDVMAELSESE"
     gene            684..1160
                     /locus_tag="VHI09_000980"
     CDS             684..1160
                     /locus_tag="VHI09_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEQ6120864.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            1218..2399
                     /locus_tag="VHI09_000981"
     CDS             1218..2399
                     /locus_tag="VHI09_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ6120865.1"
                     /translation="MSLIPEKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            2389..3636
                     /locus_tag="VHI09_000982"
     CDS             2389..3636
                     /locus_tag="VHI09_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEQ6120866.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDLEGLTEVAREKLLQAVAISSEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLMYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(3695..5347)
                     /gene="fbp54"
                     /locus_tag="VHI09_000983"
     CDS             complement(3695..5347)
                     /gene="fbp54"
                     /locus_tag="VHI09_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEQ6120867.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRLQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKRLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSDMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATKNAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLF"
     gene            5701..6699
                     /locus_tag="VHI09_000984"
     CDS             5701..6699
                     /locus_tag="VHI09_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ6120868.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANQQNITIGIL
                     QLVTHEALDDIKRGIEDQLKKQKPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAAASKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKKLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVVDVPVKVVDIGQPLINKNVAAELGIAINREDFPSASFIEN"
     gene            7045..7914
                     /locus_tag="VHI09_000985"
     CDS             7045..7914
                     /locus_tag="VHI09_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6120869.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCFPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERFFKGVRLTR"
     gene            7911..8669
                     /locus_tag="VHI09_000986"
     CDS             7911..8669
                     /locus_tag="VHI09_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120870.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQMTITDYYQLFD"
     gene            8873..10534
                     /locus_tag="VHI09_000987"
     CDS             8873..10534
                     /locus_tag="VHI09_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ6120871.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKGKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            10667..11452
                     /locus_tag="VHI09_000988"
     CDS             10667..11452
                     /locus_tag="VHI09_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEQ6120872.1"
                     /translation="MASIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMSSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGN
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            11482..11808
                     /locus_tag="VHI09_000989"
     CDS             11482..11808
                     /locus_tag="VHI09_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120873.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            11854..13761
                     /locus_tag="VHI09_000990"
     CDS             11854..13761
                     /locus_tag="VHI09_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ6120874.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDQTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMTDYDLLTEHFRQQGGFTYESDIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFMTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLTVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVEIG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQETLME
                     EWETIAEQIES"
BASE COUNT         4268 a         2340 c         2957 g         4303 t
ORIGIN      
        1 aaaaagatgt taattaaatt taaacgacct ttaaatttaa ttatattatt tcctgtaaaa
       61 tacgaataaa taagtaggag ggtaaaaatg ctaacatatg acgagtttaa gcaagcaatc
      121 gaccgtgaat atatcacagg agacacagtt atgatcgtgc gcaagaacgg acagattttt
      181 gattatgtgt tgccgcatga agaagtgaga aatggggaag ttgtgacaat cgagcggata
      241 tcagatgtta tggcagaact ttctgagtct gaataatttt agttcaccaa agaatcaaga
      301 aagaaccatt ggggcaaaat tggggcataa gtttagaact tttgtcgttt cttgtgtcga
      361 tacaataccg gtttaatact aacaaatgct tttattatcg actttgtcac cattattgtt
      421 ccttgtgttt tttcgggaat agcctcaaga agtgattaaa taaaacatta aagagccttg
      481 tcatatcaat tatcgttgat atgacaaggt tcttttgata gtttaaattg atgtttttca
      541 ttgtaggctc ttatctacac ttttttaagg catctttaga aggttttgag cgtgactaag
      601 tattcacact ttgactcata aaatgatagc atattttcta gagattggta taatagaaac
      661 aaagatggaa aggaaagaga tagatgactc aattagcaac catttgttac attgataatg
      721 gcgattcttt gctattatta caccgcaata aaaaagaaaa tgatgtccac aaaggcaagt
      781 ggatttcagt tgggggtaag ctcgaggctg gtgagacccc agatgagtgt gcccgtcgtg
      841 agattttaga ggaaacccat ttgactgtta cagagatggc ctttaaagga atcattactt
      901 ttccagaatt tacccctggt catgactggt atacctatgt ttttaaagta acaggatttg
      961 aaggagacct catttccgat gaagagtcac gagaaggaac cttggaatgg gtaccttacg
     1021 atcaagtgtt ggaaaagcca acttgggaag gtgattatga catttttaaa tggattttag
     1081 aagaccgtcc ttttttctct gccaaattta cctatgacca aaacaaccaa ctcatggata
     1141 aatctgtgac cttttattga tttacccagg ttaccagtaa aacttatcat tttctcaaag
     1201 aaaggaaccc tatcttaatg tccttaatac ctgaaaagtc tagaacagat agcttatttt
     1261 acaaatggtt tttaaataat caagctacca tggctttagt aataacactt ttagcctttt
     1321 taacgatttt tgtttttact aagatctctt ttttatttat gccagtgatt tctttttttg
     1381 cggtcatcat gttgcccttg gttatttcga ccattctgta ttatctgacc aaacccttag
     1441 ttgaccttat taatcacttg ggcccgaatc gtaccacctc tatttttatt gtttttggtt
     1501 tgattacctt actttttgtc tgggcgattt caggctttgt tcccatggta cagacccaac
     1561 ttaccagttt tattgaggat ctgcccaaat acgtgggcaa ggtcaatgaa gaagccaata
     1621 aacttcttga aaatgaatgg ttagtcagct acaagcccca actccaagac atgcttaccc
     1681 atacaagtca aaaggctttg gattacgcgc aaagtttttc aaaaaatgct attgactggg
     1741 ctggtaattt tgctggtgcc attgctcgga ttaccgtggc tattattatt tcgcccttta
     1801 ttctctttta ttttttacga gacagtagcc acatgaaaaa tggccttgtg aatgtcttac
     1861 ctctcaaatt acgggtgcct atggttcgag tcttgggaga tattaacaag caattatcag
     1921 gctatgtcca agggcaggtt acggttgcaa ttgttgtcgg ctttatgttt tctatcatgt
     1981 ttagccttgt gggactgaaa tatgccatca cttttgggat tattgctggc tttcttaaca
     2041 tgatacctta cttaggaagt ttccttgcca tgatacctgt tgtgattatg gctatggtac
     2101 aaggcccatt tatgttggtt aaggtgcttg ttatctttat gattgagcaa accatcgaag
     2161 ggcgttttgt agcaccgctt gttttgggaa ataagctgag cattcatcct attaccatta
     2221 tgttcttgtt attgactgca ggctctatgt ttggtgtctg gggtgttttc cttgtcattc
     2281 caatctacgc ttctgtcaaa gttgtgatta aagaattatt tgactggtac aaaaaagtta
     2341 gcggtttgta tgacgaagaa gtattggtga ttgaggaggt taaggaccat gttaaatagt
     2401 gaaaaaatga ttgcttcctt ggatcagcaa gacctagcgc acgctgaaaa atatttccaa
     2461 aaagctttaa aagaagatga tgcagatagt ttaatcgcct taggagaata cttagaaagt
     2521 ataggttttt tgccacatgc caagcgtatt tatttgcagt tagctgatga ttacccagaa
     2581 ctcaatataa atttagctca gattgcagca gaagacgacg ccattgaaga agctttcttg
     2641 tatttggata aggtttcaaa agatagccct aattatttgt cagctctctt agtcatggct
     2701 gacctctatg acctggaagg actgacagag gtggcgcgtg aaaaattgct tcaagcagtg
     2761 gccatcagct cagagccact ggtcattttt ggtttggcag aaatcgacat gtccttgcag
     2821 tattttaaag aagctattga ttactatgca cagttagaca atcgtcaaat ccttgaattg
     2881 acaggtattt ccacttatca acgcattgga cgagcctatg ctagcttagg gaaatttgag
     2941 gcagccatcg aattcttgga aaaggccgtg gccattgaat acgaagatga aactgttttt
     3001 gaattggcga ccctcatgta tgaccaagaa aattatcaaa aagccaatct ttactttaaa
     3061 cagttggaaa ccatcaatcc agattatcca ggttatgaat atggttatgc tttgagtctt
     3121 catgaagagc ataagacgtc agaagctttg cgcttggttc aacagggcct acgtaaaaat
     3181 gcatttgaca gtcaattatt attactagca tcgcagcttt cttatgaatt acacgatagg
     3241 cagaatgctg aaaattacct gcttcaagca aaggaagtag ctgttgatga cgaagagatt
     3301 ctcatgcgtt tagtgaccct ttattttgat gccgagcgtt ttgaggaggt tattgctctt
     3361 aatcgtgaga cgattgataa tgtcttaacc aaatggacca ttgcaaaggc ttaccacgcc
     3421 ttggaacaag aagaagttgc cttggctctt tataatgaga tttcggctga tttggcagaa
     3481 aatccagagt ttttacagga ttatgcctat ttattaaggg agtttggcca gtttcacaag
     3541 gctatccaaa tggctaccgc ttacctcaga caagtacctg atgatgtgaa tatgcaagac
     3601 tttcttgacc atatcaagca tcaacaatca gaataaaaaa acagttctcc taggggaaat
     3661 aactttccta ggagagctgt tttttagttg cttatcagaa taatttcata gagagaattt
     3721 tggcttggtc aggagtaacc cgtaaggttt tttgaccagt ataagtcaca aatcctggct
     3781 tggcgccact aggtttgtgt aattttttag cttcaatcat atcgacctga acgagatttg
     3841 ataacctagc tttcgaataa taagcagcta gttcggcagc atcagtttta acctcgtcac
     3901 ttgggtcaag attgtctttg atgatgacat gacttccagg gatatcctta gcatggaacc
     3961 agagttctcc ttttttagcc attttaaagg tgagttcttc attttgcaag ttattgcgtc
     4021 ccaccatgag aatggttttt ccatctgaag ccaggtactg ctcaggcttt ttacgtttat
     4081 ggcgtttgtc tctttggcga cttttcaaaa agccagcctg atataattct tcgcgaatat
     4141 cttcgatgtc gtcgatactt gcttgagata aattgtagtc cacgctctca aagtaagtaa
     4201 tactttgctt ggtatcggca atcagtccag ataagtgttt gacagcttct tttagctttt
     4261 ggtatttttt aaaataacgt tgagcatttt gatttggtgt cagagccttg tctaaggcaa
     4321 tctcaatttt ttcccctgta taataattgt ctaaaatcac agagtcttgg ttgtttggga
     4381 ccagactgag gtaggtggtc aataattctc ctttttgacg gaataattca gcatttttcg
     4441 tagccaaaag ttcagcttct tgtttactta gtttgttgcg atttttgtcc agttcagttt
     4501 gcacacggtg aatcaaatca ctggcttgct gattgatacg gtcgcgctca gccttgtctt
     4561 gataaaagtg gtcaagcatg tcagacaaag tctcaaacgt cgcatggcta tctgagaaaa
     4621 gaacaggcgc aaaagaagct gtggtcagat ttgcctgagt aggtctagca aaaaactcac
     4681 ggaaccgttt taatttgtct gtggtcagaa gctcggccaa ctcttttgcg gtgtctcgcc
     4741 ctaagccttg aaaatgctgt tgcaatcttt taactgttag ttcttgtgtt tggaggattt
     4801 caaatagagg aacatctgta attgtaaaag gattaacagc agctgtttta gggggctcaa
     4861 tgtaggtaga gccaggtaaa atggtgcggt aagagttttg tgaaaagcca acatgtttaa
     4921 tggattcgat gattttattt tctgcacggt ccaccaagat gatattgctg tgcttcccca
     4981 tgatttcaat gattagggtt gcctgaatag catctccgat ttcattttta ttagatacct
     5041 tgatttcgat aatgcggtca ttatcaatct gttctaactg ctcaatgact gcaccctgga
     5101 ggtatttacg cataatcatg gtaaaggtat taggtacctg aggattttga aagtccgctt
     5161 gggtaatttg aagtcgtcca aaaacaggat gggcagacaa tagcaattta taattcttgc
     5221 gatggttacg aatggttaag acaagttccc gttcaaaggg ttgattgacc ttttggatac
     5281 gaccgtaaag caagttctct tttaattcgt tggttaaatg gtgtaagaaa aagccgtcaa
     5341 aagacatggt ttctcctttc tgaattctaa tctcttcatt ataccacaaa tacttgacta
     5401 aaaatatgga cgaaagagct tgcaattttt ttgatttagg ttataatgtt aaaaaaacga
     5461 gaaaggaagt caatgatggt aaaaacacaa cacttagcaa attggcaaag ctggcataga
     5521 taagttgtgt ttatgaattg gcatagatac gacttttaga gtacttctaa aaagatatct
     5581 atgcgctgac attggatttc acaagagcgc ctagtaatca ttactaagtg ctttttcttt
     5641 tgagaagtat tctcatcatc aacgttttaa agagattgac atgaaatgtg aggaattcag
     5701 atgaaaaaca agagtttaat agctaccctc cttgttttaa ctgtaatagt gataggctct
     5761 ctattatcaa aaggagtatc aaaggaaaac cgtgatttgg ctaaccaaca aaacattaca
     5821 attggcatct tacaattggt tactcatgag gctcttgatg atattaagcg aggtattgaa
     5881 gatcaactca aaaagcagaa gcctcaaaaa caaaatgtag tgattaaggt tatgaatgct
     5941 gagggggatc agagtaaaat ccaaacaatg agtaggcaat tagtgcaatc gggaagtgat
     6001 atcgtgattg gtatcgcaac cccggcggcg caaggcttag cagctgccag caaggatatc
     6061 ccagttgtca tgtcagcagt atctgatcca gtaggatcta gattagtgat gcaattggat
     6121 caacctgaag ctaatgtaac tggtctatct aataaagtgc ctgttaaaca gacaattgat
     6181 ttaatgaaaa agctgacacc acatgtcaaa acagttggaa ttttatatgc aagtaatgag
     6241 gataattcct tatctcaagt taaagagttt aggcggttag ctcgcaaaaa gggctatcaa
     6301 gttatttcat acgcggttcc ttctactaac gaagttcctg ccacgatgtc agttatgtta
     6361 ggtaaagtag acgcggtatt tatcccccaa gacaatacta tagcaagtgc attttcatca
     6421 gtcatgacaa ccagtaaggc tgcaaagatt cctgtctaca ctagcgttga tcgaatggtt
     6481 gaaaaaggtg gtttggcagc tatttctcaa aatcagtacg atttgggagt tcaaacagct
     6541 aatcaggtgt taaaattaat caaaggaaag cgtgttgtag atgttcctgt caaagttgtg
     6601 gatattggtc aaccgctaat taataagaat gtagcagcag aattaggtat tgctattaac
     6661 agggaagatt ttccttcagc ttcttttatt gagaattaat acaatgacat gtgatataaa
     6721 aggtcaagaa aaaaccataa aaaacaccct cactgaggtg aatttggatg ccaagtcaga
     6781 caatcgtctt gaggatgtaa tggttatttt tatcattttt agttttaaaa tgctctattt
     6841 tagacacgta aaaaatcttg ccttttgagt aagtttaaac atgattctcg acttttttgt
     6901 tttctgtctt agtttcgtca aagtgattga aaaaaaagac taagccacgg tgctgaacta
     6961 agtataaaac aaatctaaaa attaatgtaa agaaagggtt tatactggcg agcgttaccc
     7021 gctaatataa atatgaggaa aaatatgatt atttcatcag tttcacaagg ccttatatgg
     7081 ggagttttag gattaggaat ctacctgacc tttaggattt taaacttccc tgacatgact
     7141 acagaaggtt cttttccttt aggaggagct gttgcggtca ccgctattag cttagggtgg
     7201 aatccatttc tatcaacctt acttggtatg ttatcaggtg ctttagcagg ttttctaaca
     7261 gggctgttat atacgaaagg aaaaatgcca acacttttag ctggaatttt ggtgatgacg
     7321 tcatgtaact ccattatgct aatggtaatg ggaagagcaa acttgggttt acatgatcat
     7381 aaacgtattc aggattgttt ccctttttca attgatttaa atagcctttt aacaggtctg
     7441 ataactgttg tgattgttat tagcgttttg atctatttcc tttacactaa tctaggtcaa
     7501 gcttatattg ctacgggaga caataaagat atggctaaga gttttggtat taacacggat
     7561 tggatggaag ttatgggact tgtggtttct aatagtttaa tagctttatc aggtgctcta
     7621 gtgagtcaac aagatggcta tgcagatgtc tcaaaaggga ttggagttat tgttattggt
     7681 ttggcgagca taattgttgg tgaagtacta tactcaactg gactgactct tttagaacgt
     7741 ttgattgcta ttgtaatagg ctcgattctt tatcaatttt taatttcagt agttattacg
     7801 ctaggtttta atacaagtta tttgaaatta attagtgctc ttgttttagc actgtgcctc
     7861 atgataccag tggtaaaaga gcgcttcttt aagggagtga ggttaacacg atgacaaaaa
     7921 ttattgaatt gatcaatgca acagttgacg tagataatgg ttttgaagat gctaaaacaa
     7981 tattggacaa tgtgacacta actatctatg aacatgattt tttaactatt ttaggaggaa
     8041 atggagctgg aaaatcaacc ctttttaatg taattgcagg aacgctaagt ttgacacggg
     8101 ggcaaattag gattcttggt caggatgtga cgcattggcc ggcagaaaaa agagctcttt
     8161 acctatcgcg agtctttcaa gatcccaaaa tgggaacagc ccctcggatg acagtggctg
     8221 aaaatctctt aatcgcacgt caaagaggag aaaaacgctc cttggcttct cgaaaaataa
     8281 cagagcactt agctagtttt gaggatttgg ttaaacgaac aggaaatggc ctagaaaagc
     8341 atttagaaac accagcaggt cttttgtctg gtggacaaag acaggcttta agtttattaa
     8401 tggcaacgtt gaaaaagcca gctctcttat tattagatga acatacggct gcccttgacc
     8461 ctaagacgag tcaatcttta atgcaattga cagatgagtt tgtgacaaaa gatggtttaa
     8521 cagctttgat gattacccac catatggaag atgcattaac ttatgggaat cgtctgattg
     8581 taatgaaaga tggaaacatt attaaagatt taaatcaaat ggaaaaagaa caaatgacga
     8641 ttactgacta ttaccaacta tttgattaat caacgatcta aatcattgac gaataataag
     8701 tcagtgattt tttgatttcc gtactgaaaa taatctgaaa gtatcaaggc tatttagaga
     8761 aaaaacgcct caaatatgat atactataga agatagtagc atagctacat aacagtaatt
     8821 cgacttacgt tgtaaggacc gtttgtaata ataaaatatt taaaggagta tcatgactga
     8881 tattaaaatg attgccttgg gaggggtccg tgaatacgga aaaaacttct atttggtaga
     8941 aattaacgat tctatgttta ttttagacgc agggctcaag taccccgaaa atgagcaact
     9001 tggggtagat ttagtcatcc ccaatttaga ttatgtaatt gaaaacaaag gtaaagtaca
     9061 agggattttc ttgagtcatg gccatgctga cgccatcgga gcccttcctt atttgttagc
     9121 tgaagtatca gctcctgtat ttggatctga attgactatt gaattagcca agctatttgt
     9181 gaaaagtaat aacagcacta agaaatttaa taattttcat gtggtagaca gtgatactga
     9241 aattgaattt aaagatggcc tagtttcgtt ctttagaaca acgcattcaa tcccagaaag
     9301 tatgggtatt gttattggaa cagataaagg aaacattgtt tataccggtg acttcaaatt
     9361 tgatcaagca gcaagagaag gttatcagac ggatttgcta cgtttagctg agattgggaa
     9421 agaaggggtt ctagctcttt tatcagattc agtcaatgcg acaagcaacg atcagattgc
     9481 cagcgaatct gaagttggtg aagaaatgga cagtgttatc tctgatgctg atggccgtgt
     9541 cattgtagct gcagtagcct caaatcttgt tcggattcag caagtatttg attccgccac
     9601 tgcccatggg cgtcgtgttg ttttaaccgg aacagatgca gaaaatattg tcagaacagc
     9661 tttacgtcta gaaaaattaa tgattacgga tgagcgtctt ttaattaaac ctaaggatat
     9721 gtctaaattc gaagaccatg aattaatcat ccttgaagct ggtcgtatgg gtgagccaat
     9781 taatagctta caaaaaatgg cagcgggacg tcaccgttat gttcaaatca aagaaggaga
     9841 ccttgtttat attgtaacaa caccaagtac ggctaaagaa gctatggtgg cgcgtgttga
     9901 aaacttgatt tacaaggccg gtggttctgt taaattaatc actcaaaact tacgcgtttc
     9961 aggccatgct aatgggcgtg atttgcaact attgatgaat ttgttaaaac cccagtatct
    10021 ttttccagta caaggagaat atcgtgattt agctgcccat gcaaagctag ctgaagaagt
    10081 tggtattttt ccagagaata ttcatattct gaagcgtggc gatatcatgg tgcttaatga
    10141 cgaaggtttt ctccatgaag gaggtgtacc tgccagtgat gtcatgattg acggtaatgc
    10201 tatcggtgat gtgggtaata tcgttttacg agaccgtaaa gtcttgtcag aagatggtat
    10261 tttcattgtt gctattactg tttctaaaaa agaaaaacgc attatttcta aggctaaagt
    10321 taatacgcgt ggatttgtgt atgtcaagaa aagtcatgat attttaagag aaagtgcaga
    10381 attagttaat acaactgttg gcaattactt aaaaaaagat acctttgact ggggcgaatt
    10441 aaaagggaat gtcagagatg atttgtctaa attcttattt gaacaaacca aaaggcgccc
    10501 agcgattctg ccggttgtga tggaagttcg ttagtgaaaa taggaaggtt aggcaaaaac
    10561 tgcctacctt ctttgatttt aacaactggt aaaccaggaa actgttcacc caaaaataca
    10621 gtagatttac gtgataggat tattttggta taaaggagtg taatgaatgg caagtattgc
    10681 aattgaatac cattcggtag ttttaggaat ggagcgtaag gtaaatgtga tttatcctga
    10741 ccagtctgaa atccctaaaa aagatcaagg ggataaggat attcctgttt tatatttgtt
    10801 acatggtatg ggaggcaatg aaaactcttg gcaaaaacga acagctattg aacgcttgtt
    10861 gcggcatacc aatttgatag tcgttatgtc ttcgacagac ctaggttggt atacagacac
    10921 ggcttatgga cttaactatt atcgggcgtt gtcacaagag ttaccgcagg tcttagcagc
    10981 tttctttcct aatatgaccc agaaacgaga aaaaaccttt gttgctgggc tctctatggg
    11041 tggctatggt gcctttaagt gggcactcaa aagcaatcgg ttttcttatg cggcatcgtt
    11101 ttcaggcgct ttggattttt ctcctgagac cttgcttgag ggaaacctgg gagaattagc
    11161 ctattggcag ggcgtttttg gccaatttga tgaccctgac cttgacaagc attaccttaa
    11221 aaatatggtg gcagaaagtg atggaaaaac caaattttat gcttggtgtg gctatgagga
    11281 ttttcttttt gccactaatg aaaaagctat tgctgatttt caggctcaag gtcttgacat
    11341 tgattatcat aaaggtcatg gtaaacatga gtggtactat tggaaccagc aattagaagt
    11401 cctgttagag tggttaccga taaactacca aaaagaagaa aggttgtctt aaccatttcc
    11461 tagtacaaca tgaggtgaca aatgaaacaa ttactggcga tcattttgtg gctgcctaag
    11521 ctaatagtga aaatgttttg gcatcttatc aaagggtttt tacaaactat cttattagta
    11581 acgattatta ttatagggct gatgtattat tctaatcaca gtgattccgt tttagccaat
    11641 aagatctcga tagttactga gcaagtggtg caaatttttg atatcttaac acaaaagcca
    11701 tctgcaaaga cgcgtcatgg gagtggtaat agtcattcca ctttccttgt tagcgctaaa
    11761 gaaggtaatc aacgacttaa aaagagttcg atttcttttg gaaactaggc tgaaaatgtc
    11821 ctcaaaaggg catttttttg ttagaatgaa agtatgatta ttttacaagg aaacaagtta
    11881 gaacgctctt tctcaggaga tgttcttttt caaaacatat cactgcaagt tgatgaaaga
    11941 gacagaattg cattagtagg gccaaatgga gctggtaagt caaccttact caagctattg
    12001 gttggggaag aaaccccaac gagcggagaa gttaatacta aaaaagacct aaccttgtct
    12061 tatctggctc aaaatagccg ctttgaatca gatcaaacaa tatatgagga aatgctcaag
    12121 gttttcgaag cattacgcca agatgaaaag cgcctgcgtc agatggaaat ggatatggca
    12181 accgtttcag gtcaagattt aacccgactg atgacagatt atgatctgct tacagagcat
    12241 tttcgtcagc aaggcggctt tacgtatgaa tctgacatca aagccatttt aaatgggttt
    12301 aagtttgatg agagcatgtg gcagatgact atcgctgagc tgtcaggggg tcaaaatact
    12361 cgtctggcct tggctaaaat gcttttagaa aagccagaac tattggtcct tgatgagcca
    12421 acaaaccact tagatattga aaccattgcc tggttagaga actacttggc caattatcaa
    12481 ggagctctaa tcattgtgag tcacgaccgt tattttttgg ataaggtggc aacagtgacc
    12541 ttggatttaa ctcctcatgg attggatcgc tacgttggca attattctcg ttttatgact
    12601 ttaaaagctg aaaaattagt agccgaagaa aagcagtttg acaaacagca aaaagaaatt
    12661 gccaagttag aagattttgt tcaaaaaaat attgtcaggg cttcaactac aaaacgtgct
    12721 caggcgagac gtaagcaatt ggaaaaaata gaacgcttag acaaaccgac tagtgctaga
    12781 aaatcagctc acatgacttt tcaggcagat aagccgtctg gcaatgtggt gttgacggtt
    12841 gaaaaagctg ctattggtta taaccaacat gttttatctg agcctattaa cctagacgtt
    12901 cacaaattag atgcgattgc tattgttggt ccaaatggta ttggtaaatc cactctgatt
    12961 aaatctgtta ttggacagat tcctttcatc aaaggagaag tgaaatacgg tgccaatgtt
    13021 gaaataggtt actatgacca aacccagtca catttgacgt ctagcaacac agttttggaa
    13081 gaattatggc aggatttttc aactacgcca gaagtcgata tcagaaaccg attaggggct
    13141 tttctctttt ctggagatga tgtcaaaaaa tcggtggcca tgctgtcagg tggagaaaaa
    13201 gcccgtctgc ttcttgcgaa gttatctatg gaaaacaaca attttcttgt tctggatgag
    13261 ccgactaacc acctagacat tgacagtaaa gaagtgctag agaatgccct cattgacttt
    13321 gacggcacct tgctttttgt cagccacgac cgctatttca ttaatcgttt ggcaactaag
    13381 gttctggaaa ttaccgaaaa tggctcaacc ctttacttag gtgactatga ttattacctt
    13441 gagaaaaagg cagagctaga agaactagcc cgcctagcag caggagagac agttgaggaa
    13501 acaaaagaag cctctgccac tgattatcag ctacaaaaag ctaatcaaaa agagcgtcgt
    13561 cgcttgaccc gccgatatga ggagattgaa gcaagacttg aaaccattga agagcgcatt
    13621 ggtgctattc aagaagacat gcatgcctcc aatgatacgg cgcagttaat cgcttggcaa
    13681 aaggaatggg accaattgga tcaagaacaa gaaactctta tggaagaatg ggaaaccatc
    13741 gctgaacaaa tagaatcata aaaataagct ttagcagttt tgctaagctt atttttatcg
    13801 ctttgttact tgtttgaagg tgtgaaagag tttttaaaat gatgtgtttt tttatgtcaa
    13861 ggtcggta
//
LOCUS       DAUELZ010000044        13334 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.044, whole genome shotgun sequence.
ACCESSION   DAUELZ010000044 DAUELZ010000000
VERSION     DAUELZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.044"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            204..1082
                     /locus_tag="VHI09_000991"
     CDS             204..1082
                     /locus_tag="VHI09_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120875.1"
                     /translation="MIRKHDRTSTKKKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLAKAKIAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPKIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHEANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            1095..2357
                     /locus_tag="VHI09_000992"
     CDS             1095..2357
                     /locus_tag="VHI09_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEQ6120876.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLKEFYFDSQNIEQKMDSM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIINANNAALQMLAETSKEAIPA
                     LEQSAQNPTLSMKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTHA"
     gene            complement(2477..3103)
                     /locus_tag="VHI09_000993"
     CDS             complement(2477..3103)
                     /locus_tag="VHI09_000993"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEQ6120877.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(3148..4563)
                     /locus_tag="VHI09_000994"
     CDS             complement(3148..4563)
                     /locus_tag="VHI09_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEQ6120878.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGQPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGFYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            complement(4564..6339)
                     /locus_tag="VHI09_000995"
     CDS             complement(4564..6339)
                     /locus_tag="VHI09_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEQ6120879.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGDFTVTDTVYEIKQEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     QSNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFNEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            complement(6540..6860)
                     /locus_tag="VHI09_000996"
     CDS             complement(6540..6860)
                     /locus_tag="VHI09_000996"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEQ6120880.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDYGIIYITEDIAAAIPEALTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            complement(6857..7855)
                     /locus_tag="VHI09_000997"
     CDS             complement(6857..7855)
                     /locus_tag="VHI09_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEQ6120881.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLAALEHLIRTMTSDEFPKEVVTEIQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            complement(7871..8455)
                     /locus_tag="VHI09_000998"
     CDS             complement(7871..8455)
                     /locus_tag="VHI09_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEQ6120882.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            complement(8524..9003)
                     /locus_tag="VHI09_000999"
     CDS             complement(8524..9003)
                     /locus_tag="VHI09_000999"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEQ6120883.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            complement(9005..11005)
                     /locus_tag="VHI09_001000"
     CDS             complement(9005..11005)
                     /locus_tag="VHI09_001000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEQ6120884.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEESHRQQAAQTALKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDHFYHQLKQHKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEADSVNQLKGLMTKTLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVAIWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            complement(10995..11315)
                     /locus_tag="VHI09_001001"
     CDS             complement(10995..11315)
                     /locus_tag="VHI09_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120885.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            complement(11769..12788)
                     /locus_tag="VHI09_001002"
     CDS             complement(11769..12788)
                     /locus_tag="VHI09_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEQ6120886.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            complement(12825..13190)
                     /locus_tag="VHI09_001003"
     CDS             complement(12825..13190)
                     /locus_tag="VHI09_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ6120887.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
BASE COUNT         4101 a         2877 c         2423 g         3933 t
ORIGIN      
        1 ttttttaacc atgataaaca atcctaaaaa aacgaacatt ataagagaaa agcttttaac
       61 ctcgtttctc ctaagcttta gcagggttaa acgattctct taaaacatta gctcttttct
      121 ttcagaaata gcggttttaa ggtaaaataa tagtaagtta cctctatgag atagccagta
      181 aaaacatatc gaggaaatat cacatgatta gaaagcatga ccgcacgagc acaaaaaaga
      241 aatcactaaa ctggatttgg ctaatcattg ctttttttat gatttcaagc tttataggtg
      301 gctcttcttt tactgagtca cttctagaca ttttgcctgc catagccatt ggtggtacag
      361 gttatgctat ttttcgtgtt agatctcatc aaaagcggct agcaaaggcc aagattgcca
      421 aacaattaga agatctaaaa gcaaaaattc aactagctga tcgtaaagtt cggctgttag
      481 acacttatct ggcagaccat gatgacttcc aatataacgt acttgctcag caattattac
      541 cacaacttag tgacatcaaa gcaaaagcta ttacgttaaa agaccagtta gatcccaaaa
      601 tttatcgtcg tatcaccaaa aaagccaatg acgttgaaag tgatattacc cttcaactcg
      661 aaacactgca gattgccaca actcttaacc ctcagccttt gaaaacccca tctcctaatc
      721 ttattaacaa agcacctgag ctcaaaccgt attatgataa tattcagaca gatcatcaag
      781 ctatccttgc taaaatccaa ggagccgaca atcaagaaga attgcttgcg ctgcatgaag
      841 ctaacatgag gaggtttgag gatatcttaa caggctactt aaaaatcaaa gaagagccta
      901 aaaattacta taacgctgct gctcgtttag aacaagccaa acaagctatc caacaatttg
      961 atgaagatct tgacgaaacc cttcgtcgac tcaatgaaag tgatcttaaa gattttgata
     1021 ttagccttcg aatcatgcaa ggtgctactc aaagacgaac aacgcatcac caaaaagact
     1081 aaaggagtcc ctttatggca gattttaatt ttgacattga ccaaattgct gacaatgctg
     1141 ttgtcaaaac cgataaaacc accgatatta tctcagactt accaactgac acaaatggcc
     1201 aaatctcttt ttttgagaaa ctaagtgctg accagcagac agctattact gccaaagccc
     1261 cggcattggt agataccttt ttggcagacc aaaatgcgct acttgatttt ggtcagtctg
     1321 ctgttgaagg ggtcaatgca acggtcaatc atatcctggc cgaacaaaag aagttacaga
     1381 ttcctcaggt tgatgatctt ttaaaatcaa ccaatcgtga actcaatggt tttatcgcta
     1441 aatacaagga tgccacacct gtggaccttg ataaaaagcc aaactttctt caaaaactct
     1501 ttaaacaaag ccgtgatacc ctgaaagagt tttattttga ttctcaaaac attgagcaaa
     1561 aaatggatag tatggctgca gcggttgtca agcaagaaga taccttagct cgcaatattg
     1621 tctcagcaga attgttaatt gaagataata ctaagtctat tgaacatttg gtaggcgtca
     1681 ttgcttttat cgaagctagt caaaaagaag ctagtcagcg cgctgcggct ttacaaaaag
     1741 acctaaaaac caaagacagt gctacaccag actatcaaat aaaagctgat cttttggcaa
     1801 gaactaccga agtcatcaac accctcgaac aacaacacac cgaatacttg agtcgcctct
     1861 atgttgcttg ggcgacaact ccgcaaatgc gtaatctagt caaggtctct tctgacatgc
     1921 gtcaaaaact aggcatgtta cgccgcaata ccattccaac gatgaaacta tctattgctc
     1981 agttaggcat gatgcaacaa tcggttaaat ctggaatgac tgctgacgcc attattaatg
     2041 ctaataatgc tgcccttcag atgttggcgg aaaccagcaa agaagccatc ccagcattgg
     2101 aacaatcagc acaaaaccct actttgtcta tgaaatctgt gacaagtctg gcagaaagtt
     2161 tagtagcaca aaataatggg atcattgcgg cgattgatca tggacgcaaa gaacgagcac
     2221 aactagaaag tgctattatc aggtctgccg aaaccattaa cgattctgtc aaactacgtg
     2281 accaaaatat tgtgcaagca cttcttagtg aaggcaagga aactcaaaaa acaattgata
     2341 aaacaactca cgcataagct aaacatcacc tgtcacgcta cttaaaaagc aagccatcag
     2401 ttaggttaat ccctttgatg gcttgctttt ttactactta acgaataatc gccttgtttg
     2461 atataacact tatcttttat gatgttattt tcataatacg aacaaggcta gcacgctcgg
     2521 cctcttctaa tttcaactca atgtagtgaa tcgtctcttc cagttgcggg ataatagaat
     2581 actctaagcc attgacgcga cgtctagtct tttcaatgtc atcagccatt agttgacaag
     2641 ttttttcaac ttcagctaac cgtaagagct tttctaatag ttcttttgtc ttttggatag
     2701 tattgtccat ctcactattt gagctgaggt aactataagc aaactcacct tgttcttgct
     2761 ctcttgccgt attagactgg acatggaatt taggcacatt gacactcatg atattttcaa
     2821 tatcaatcca taaggtcact tcatggactg gaacagcaaa gagttcttct accattagac
     2881 tattttcgga tgcttttgcc aagacaaact ccttcatatt ggcagcaagt tctttttcga
     2941 tagtttgacg aagctcattg ttttcacgaa ttaaatcaac gaagcggcgc atcaattcat
     3001 ctcgcttgtc ctttaataat ttatgacctc ttgtagctgt cttcaagcga tttttcaagt
     3061 tactaagttc catgcgtgtt ggtttaacat ttaatcgtgc cattgatata actcctttcg
     3121 tcaagggctt gaaattgcat ggtcaactca atcatttgca acaaaaacct ttgtcatcgt
     3181 ggtatctgct ttaggcaaat aacgatctaa catatcatct ttaatgcgtt ttaattcggt
     3241 acgtggcaaa atcgacaaca attcccaacc caagtcaaga ctttcttcaa tgctacggtt
     3301 ggtgtaaaat ccttggttaa tgtattcttc ttcaaaccgg ttggtaaagg ccacatatag
     3361 tttatccgtt tcagaaaggg ctgattcacc gagcacaaca gccaattcct tagcttgttt
     3421 tccttgggca taggctgcaa agagctgatt cattgtggca gcatggtctt gtcgagtctt
     3481 accttcacct gaacctttat cttttaggcg agatagcgat ggcaagacat taataggcgg
     3541 tctaaaacca tttttatata gttcttgcga caaaataatc tgaccttcgg taatataacc
     3601 tgtcaaatcg gggatagggt gggtaatgtc atcttctggc atggttaaaa taggaatttg
     3661 agtcactgac ccttttttcc cgatcaagcg accagcccgt tcatataagg tggagagatt
     3721 agtatagaga tagccaggat aacctcgtct gcctggaact tctctgcgag cggctgatac
     3781 ttctcgtaag gcttcacaat aatttgtcat atctgtcatg atgactaaaa catgcatacc
     3841 tttttcataa gccaagtatt cggctgttgt cagagctata cgtggtgttg caatgcgttc
     3901 aatagcaggg tcattagcta aattaataaa taaaacagat cgatcaatgg cacccgtttc
     3961 acgcaagtca ttcataaaga attctgcctc ttcaaaagta atacccatag ctgcaaacac
     4021 aaccgcaaag ttatcatcag agtttagaac agttgcctga cgagcaatct gtgcggccaa
     4081 ttcattatga ggtaaccctg agcccgaaaa aactggtaac ttttgaccac gaacgagggt
     4141 attgaggtga tcaatagctg agatacctgt ctggataaat tcgtctgggt aatcacgagc
     4201 gacaggattg atcgcttgac catcaatatc aaggtatttt tcgggaataa gttctggacc
     4261 gccgtctatg ggctgcccca ttccatcaaa gatgcgccct accatatctt ctgatacggc
     4321 taattccaaa ggatgccctg taaatcgaac cttggttttg gctagattga tgccagttga
     4381 tccttcaaat agctgtacca tagccttgtc accttggact tctaagactt gacctttacg
     4441 cctttctcca ttatgcaaag taatatctac caattcgttg tagtgcactc ctgcaacttg
     4501 atcaacaatc atcaaaggac caaccacttc gctaaccgtc cgatattctt tgagaacgct
     4561 catctaaatt cctccctttg ctaaaacgtg gtgaatctct ttggtaacct tctgagtaag
     4621 ccctttaatc tgctctaagt tttcttctgg gatgaatttg ctgcgtgcga tgcgatcgcg
     4681 tacctgagca gtgccttcca taatctcatt aaaataagct cccaaagaaa gggctttgct
     4741 ggcttcctca ttaaaggtca aaatattggt tagcatggcc tcttgtttcg gaaaggaagt
     4801 aaaggtatcc accgaatcaa aggcattttg ctggagataa tcctcccgaa tttgccgggc
     4861 aacagccatg gtcaaacgat cttgttcaga cagtgaatca agccccacca agcgtacaat
     4921 ttcttctaga ctggcttcac gctgcaaaat agccatggca cgagttacct tgttggacca
     4981 attagattgc tgtttacggt caatatagct tcctacatca tcttgataca atgaataaga
     5041 cgtcagccag ttaatcgctg ggaagtgacg ccgttgcgca agaggcgcgt cgagccccca
     5101 gaaaactttg acaatccgaa gggtgttttg agtgacaggc tctgaaatat cccctccagg
     5161 aggagaaacc gcgccgatgg ctgtaatggt tccctcacgt tcttgacttc ccaaggtccg
     5221 aacacgacca gcccgttcat aatattcggc aatgcgactc cctaagtaag ccggatagcc
     5281 ttcatcacca ggcatttctt gtaggcgtcc tgacatctcg cgcagagctt ctgcccaacg
     5341 tgatgtcgag tctgccatga tagccacaga atagcccata tcacggaaat attcggcaat
     5401 ggtaatgcct gtgtaaatcg acgcttcacg cgctgctact ggcatattag aggtgtttgc
     5461 aattaacacc gtgcgctcca taatggactg gcctgtattt gggtcaatta actctggaaa
     5521 ctcattcaaa acgtcggtca tctcgttgcc gcgttcccca caaccgacat aaataacaat
     5581 atcaacgttg gcaaatttag ctatttgatg ctgcacaact gtttttcctg ccccaaatgg
     5641 tccaggaacg gcagcggcac caccttttgt aacagggaaa aaggtgtcaa taacccgttg
     5701 acctgtaacc aaaggctcga ctgggataag cttttgagca acagggcgac tttgacgaac
     5761 tggccaagtc tgcatgaggc taccttggta aatggaaccg tcttcctgct taatttcata
     5821 aactgtatct gttactgtga agtcccctgc atgaatagcc accaaggtcc ctgaaacttt
     5881 ataaggaacc atgatacgat gctcaatgac agctgtttct tgcacagttc ctaaaatatc
     5941 acctgcaact acttcttgac caacacttag cttgggaata aatgcccact tagcctttcg
     6001 atcaagactt gggatagcca caccacggat taaaaagtcg ctgtccgttg ctttttgaaa
     6061 acgatcaagc ggtcgctgaa taccgtcaaa catttctgaa atcaggcctg ggcctaactc
     6121 gaccgacaaa ggacaaccag tagtcactac tggttctcct ggaccgatcc ctgatgtttc
     6181 ctcataaacc tgaatagacg cttgatcgcg gcgcatttca ataatttctc cgactaagcc
     6241 aagatgtccc acacgacaaa tatcttgaat attagcttct tgcatcccag aagccacaac
     6301 aagaggtccc gaaacggtta ttatttttcc ttggttcacc tttttcctcc tttatttttt
     6361 ccaaatcata ctaactaatc tatactgtca aggtttaatg tgactatttt gaatagtcag
     6421 caaactatcc tatgcgttca gctgctaact tcaccaattt tcctgtcctt gcatttcatc
     6481 agttagctac tcataagcct caaacagagt ataagtcctc tttgcataac gtcttagtgt
     6541 tataaaatat tttgaccgac agccttttca accatggctt gtatgcgtga caggcctatc
     6601 ccttgcgctc cttgatgagt tggtaatggt atgactgctg gtagaacttg attgtcatag
     6661 tgagtgaggg cttctggaat agctgcagca atatcttctg tgatataaat aataccatag
     6721 tcttccattg ctagttgacg caactggtta atagcgtctt gtggttttat tactggaaaa
     6781 gtttgaaacc caatcatctg aaaaggtaga ataacatctc tgttaccaat taccccgact
     6841 ttatacaatg ttttggtcat gctattggtc tcatcctttc tatgacacgt tctttgggta
     6901 aatcattagc caaagcagcc gctaaaagcc gtagattttt tacttcaaac gatttagcga
     6961 gcaaaaaacg agcaagcaca taaggcccat cgacttgata tttggccttc gcaaataaat
     7021 ggtaaaggca ttcatcaaca aggtattcta actcagtcgt ttgaagagtg ccttggcgaa
     7081 gtttctctga ataaggtttt agggctgtca tatagctatc aggtgttaca ttttcaaacc
     7141 acgtcatcag atccttatca tcttcaagtg tgataaatgt tttacttggt ctacttgctt
     7201 catcagataa taactgcatc atgaaactta ttggtttatt ttgcgatttg gcccgtctga
     7261 cggtaatcag attatacaaa tcaatcacaa taagaacagc ttgttggaaa acagggtcct
     7321 ctaatctttg tgctatttgt ttcaacgcct taaaataagc taagtcagta ccaatttcta
     7381 agacgcgtat gtcttgataa tcttgatatt cggcccaaat ggactggatt tctgttacca
     7441 cctcttttgg aaactcatca gaagtcatcg tcctaatcaa atgttccaaa gccgccaaag
     7501 gcttgtcacc aattggtagt aataagtgac tcagatctgc ttgactagct ttagctttta
     7561 acaacacttt gacattatga taggtgtaac gcaaggtaaa taattgtact atgtcaggtt
     7621 gaggggtttc tgcaaaagcc cagcgatagg tcttggttag ctcagccatt aagatagatt
     7681 ctagccgatc caaatcgttg agatcgtcaa cagttaggtg atagactgac tggtgcaaca
     7741 gtcgagctaa ggttgttgta ttgggagcct gcaatagctt atcaaattgc tctttagtca
     7801 ataactcttt ttctttgaca ctgatagtag tgtttaattc tagaaatgtt ggcatcgcta
     7861 gtcaaacctc ctatatctct gaaaaaagca tatcaatgat gcgggagctt tcttctttaa
     7921 gaactgattc taaaaggtaa cggtataaat aattgtcatc gacttggtct aaactgacta
     7981 aaaatcctgc ttcctgagga attagctctt gattatagct taattgagga aatgctagtc
     8041 tcagctcagc acaatcataa gaactgaact tttgccctgt tttttcgcca aaagtaactt
     8101 gcaatggctg ttctggatat ttagacaaga cttgcttcaa aaaagccaat tcttcttcct
     8161 tagaaaagtt cgtcatcttc tccaaagaag cttcaaatag ctcctttata ctgtcttgtt
     8221 ttaatgctag tagagcttgt ctttcttggt ttttgagttg ctgctgcgca acctgaaatt
     8281 gctgttcaag agcctttaac tctttttgac gctttgcttt taaatcgtgt agtccttgct
     8341 gttgacgctc tttaaagtca gtatcaagac tatctgtcgc tatctttagg cactgttgac
     8401 cttcttgatg tgctttttca agaacgtttt gtcttaactg tgttatatca ttcacctaat
     8461 ttcccccttt tgatagtgtt agtggtatca ccgtttttta aaatctagat aaggaccgtt
     8521 accttatcca attttattta agagaataaa ggagacgaca aaggcaagaa tagcataggt
     8581 ttcaaccata gctgctagaa tgacaccctt cataaaatct ttagggcgct tagccaagat
     8641 ttgcatacca gctacagaaa cattgccttg gtgcttggcc gaaaaataac caacaatagc
     8701 aattggaaga gataccaaaa agtaagctaa tccttggtta actgaaagtt ctggtgtcag
     8761 tttcatccaa attaaaatac caatagcaaa gccgtagatt ccttgactac cgggtaacag
     8821 ttgcaagata agcgcagacg taaatttttc gggttcttct tttaagagcg cagctgccgc
     8881 ttgaccacct ttaccgacac cataagctga gcccatgcca ctaagagcta cggctaaaac
     8941 aattcctaag gctgcgaaga aaacgccacc atgtgctgta aaataatgtg ctaaatgttc
     9001 catgttaatt gtcctccaaa tctgtttctt cattgacatt gacataatta tcagctaact
     9061 ttaaaggatt gaaagctttt cctccacctt cataaaattt tccaaaaaac tctacgaaaa
     9121 taagacgagc accatgcaca tatcctgaaa gcattgataa gaaaatattg atcgcatgca
     9181 aaagaataaa aataaagata cctactgtaa agcgcgtgac tggtggaaaa atcccaacaa
     9241 tcatgttaaa agccgctcca atactagctc cagaaagccc taatgccatt agacgagtaa
     9301 agctaaccaa atcacttaag taagagctaa tgccatataa attatataat ccactaccta
     9361 gacctaaaag gcttttgctt tttagtaaag aaacaaccaa aatcccaaag gcattgccca
     9421 atgcaagcca cttaccaata accgataagt aagcaaggcc agatacattc ttaccaagaa
     9481 taaataacag caggcctaat aaaataaaag tccaagctag acttgacgta taggccgtag
     9541 catatgcttt catccttact tgttgccatg ctcctaacaa aagcccaaat ataagtgtaa
     9601 caaacccaaa tagcaaagaa acaaccaaaa tagtaatcac atccgtttta gttgataata
     9661 atgccacagg taagtcaaaa ccaaagaaag aaccgtaaat aagcccccat atggctactg
     9721 aaatagctaa aatattaaag aaggtcacta accgtttact ggttttttgg agattaaaaa
     9781 agaccaatgc agctagtgtc acagcatata gcaacaaacc atagcccaaa tcagctacca
     9841 tcatgccaaa aaaagtaagg tataaaggag ctaaaaaagg tgtcggatct ttttcttgat
     9901 attttggcaa agcgtacatt tctgtaacca attcaaacgg tgcaatgtag cggtgattac
     9961 gtaacttaat gggaacatct tcccaatcgt ctggcgtgac atcgtaacta tccagataaa
    10021 acatatcacc tagtgtctta gtcattagcc cttttaattg gttaaccgaa tcagcttcta
    10081 tccacccttc taaggctatt agatgtcttg taccgagtaa ttgcttttta gtttgctgcc
    10141 gttgatactg actaaggaca tagtctattt caacctgtaa ctgtgccagt atcttttgag
    10201 actgcctaag ctcttttagt agactatctt tttcagctag ccaattcgtt agtaactctt
    10261 tgtttatacg aagttgttct gatggcagta gctgttcttt atataataag ggtttaaact
    10321 gatatttctg taaatgaatc gtatcttgtg cttgccaaaa taagacaaga ccgtactcaa
    10381 attctgtatg gtatacttcc tcaataaaaa gacctttgtg ctgctttagc tgatgataaa
    10441 aatgatcatt tgctgtacta ggaatcgttc caactcttgc cgacaaaaaa tgaaattgcg
    10501 ctaaatcctg aggcaaaaca gtcaaggaac gccacttttc taactcctga cagtattctg
    10561 ttagctggtc tattttgttt tgcagctgct ctagtcgctc tatcttttgt gacatcattt
    10621 tcaaagccgt ttgagctgct tgttgtcgat ggctttcttc gaactgttta aacgaaagag
    10681 atggcgtttt ttgtctcaag gctgttaact ttcctattgg tggtaaaaac gctgataagc
    10741 tagcaaggcc attttctaac tcttgttggc tttgtaaaag gtattgtaaa gcttcatcat
    10801 ctagtagagg tttttgatgc gtcagatcgt attggacgat ttgtggcaac ttgacgttgc
    10861 ctttgttaaa agcatcttgc caatgtttta gttgcttcat gtcacgaact tcaactaatt
    10921 gagattgttg aagtgttttt aaaacaagat caagataatc tttttcaaaa accattgcca
    10981 gctttttcat ttgactaatg gccatatgtc tccaccacct tatccactaa ctccctaaca
    11041 acggttagct tatgctcttc taaaatggca tgaagggcta gctgttgcgt cgctaacctc
    11101 ttttttgaag ctttttgttc ttgacgatta gcttcaaaaa acacctcacg tttagcttct
    11161 aatagtagct gagtttcttg ttcgtactca aggaacagct gctttaactc ttcttctttt
    11221 ttgtcagcta gagctttttt cttagcatcg taactgtcat aaattgttct agcctcttgt
    11281 tcgatactat tcattagtga caatagggta ttcatcacat atcaactcct tatctttatc
    11341 tattgctaat tctttgactc actaggtact taaaaacaaa gctacaagaa aggctacttt
    11401 tgggggttaa tcaaccagcg actataagct agagctaaat ctctaaccta tctactaaca
    11461 gcttttttga gataggataa ttgcttgatt aaacgaacag catctttaag ttaagcgctt
    11521 tcaattttat ccataaaaac aatggcttag ttcagaaaaa ggttagctaa aaacctgtca
    11581 aagcagtaag atgacacaga actctaaagc tttgtattgc tgtctttcgt gtgttatctt
    11641 atacttcttt aggcatttag taaccttgat ctagttgacg gtaaggtacc tttaccagcc
    11701 aatagccaag ccaatgtgat aacagtataa ctgctatctt gttaaaagtc ctgtcaaaaa
    11761 gatgtttatt agtttctcgc ttgtaattct tgttttgtta ctaaacggta atttttaaag
    11821 acagaacctc caatataacc cacaattaag ctgataataa tacatccaag ggctgttatc
    11881 aacactttag cgacaggatt ataggcaaac atcaccgcaa aaccggcaat cggagtagct
    11941 gtccctggcg tatcattaac caatttcatt agggcgatca aaacaccaca tgcagcacca
    12001 ccaacaaaat tagtcacata aatcggaatg ggattggctg acgtcacatc tgcctgcgtt
    12061 agtggttcaa tagcaaaagc aatattgtct tttctttcac ccaattttaa tcggtaaaaa
    12121 agaacaccat tcataaaaga agagccaaag acagataagg cacctatagc catcggaata
    12181 cctgtcagac caagcatggc tgtcaaagcc attgatgata gcggagctgt tgctacaaca
    12241 acaatcgtac cgcctagtat cattcccatg ataatagggt tgctattagc acttgaggtc
    12301 aggatgtctc caatagttaa taaagtacta ttgatcactg gcgtaattaa ctgcgccaag
    12361 aaacgagtta atggtgcacc gacaataata atcgagatta aatcaagccc accaggaata
    12421 tttttttcca tccacttgat taaaaaagat accacatacc cagccacaaa acctgggata
    12481 atacctgtcc ctgaaactga caaaccaatc aaaacggcat aacctggaga cacaccaatt
    12541 gcaatggcaa ccaagatagc agcagctacc cctgataatg agccatttgc tccgcctact
    12601 tcacttagaa actgaaagcc taaaacctta ccaaaaaaag aatcttgaaa ggcttcaact
    12661 aaaaaagagg cacaagctgc cgaagccaaa gctcccattg ccttggcacc atgaggagcc
    12721 ttatagttaa acaagctaaa aatagctaaa acaagaatca aaagacttgt tccaataata
    12781 atatccataa tctattccac caatctatct cattacataa gacactataa ctgcttcact
    12841 ctggcaacag cttcgacttc aatcaaagct tgcaaaggaa gcgccgacac ttgaaccgct
    12901 gtccgaacag ggtaaggctc ttcaaacaaa tcagtcatga catgatttaa tatttccaca
    12961 ttggtcacat ctgttaaata cacatttagc ttatagatat gattcaagtc caacttttgc
    13021 tctgccaaaa tagattgtaa attaacaaaa acctggcgac actgtgcctc aaaaccatct
    13081 gacaattgac ctgtcactgg attaaggggt aattggcctg cggtgtatag aaaatgtcct
    13141 tcaatcgtat aagttgaata agggcccatt ggttctgggt agctcttcat atacgaacct
    13201 cctcattgat aatatagtta aattttacag gaaaccgcat tcacagtcaa taataaattc
    13261 tctttttgat aaaaaattat tattaaggtg attggcaatt gttttttagt ctaaaatgta
    13321 ttactatatt tgta
//
LOCUS       DAUELZ010000045        13258 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.045, whole genome shotgun sequence.
ACCESSION   DAUELZ010000045 DAUELZ010000000
VERSION     DAUELZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.045"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            19..1266
                     /locus_tag="VHI09_001004"
     CDS             19..1266
                     /locus_tag="VHI09_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ6120888.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADIIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLTAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            1540..2901
                     /locus_tag="VHI09_001005"
     CDS             1540..2901
                     /locus_tag="VHI09_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ6120889.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPSFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINTKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            2901..3737
                     /locus_tag="VHI09_001006"
     CDS             2901..3737
                     /locus_tag="VHI09_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ6120890.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            3895..4692
                     /locus_tag="VHI09_001007"
     CDS             3895..4692
                     /locus_tag="VHI09_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ6120891.1"
                     /translation="MTSKITIKDIAREAGVSVATVSYVLNQRNDQKISEETRNKVLHI
                     SNLLNYTPNQAARSLVTQQSNSVVLYYKYADSILARAEQLHTISFLSRLLHEQDYQLV
                     CLNEDSIEKFDWADLILTLDVDNDTFYKIGKLNFKPLIAIDSFVNDPLFFQINTDYAA
                     LKDQANQFFKDEPYTLLSLPITNRERYEHVSAFFPSLHIVNDLRELTYFEKENLLLID
                     ESLDQFFVNSSQKLYCPRFSPKKGQVILEAIEWAKKRQPDKKHNLLV"
     gene            complement(4808..5623)
                     /locus_tag="VHI09_001008"
     CDS             complement(4808..5623)
                     /locus_tag="VHI09_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEQ6120892.1"
                     /translation="MPDKKQFPISYISHCLSPRAMFASRAQFTWWQNLLIIVFLNALI
                     MIPVTLHYANMTTYPLERIVTKSLSPITDKTYQALTQGKIEKDTFQGQSLIRRDGELV
                     LAVLPTKVDLEQLPSESTRQIIVTKKEWRFVTPDGKELRAHVRGQQQSLADLTTVKAV
                     KDFVNQQWYDSNKASVLGFLLLTFVLMVCVGTLIVIGLGAFFLTLTKRSRLFMIRNFS
                     EGLGLMVNCLAWPSLLAIALSFFIQDPVLIMNCQVFGTLLMLTWVFYKTQFRD"
     gene            complement(5645..6505)
                     /locus_tag="VHI09_001009"
     CDS             complement(5645..6505)
                     /locus_tag="VHI09_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ6120893.1"
                     /translation="MRKTYVSISMKRKRFFTQLLTYLYLISLVIVILFPILVTVSSAF
                     RPGNTTAFSFHFDGPWTLSNFKTLFQDTLYLRWYWNTLIVAFFTMLIQVTVITLTGYA
                     YSRYNFFGRKKSLIFFLVVQMVPTMAALTAYFVMAWLFNALNQYWFLILIYVGGGIPM
                     NAWLMKGYFDTVPYDLDESAKLDGSGHFRTFYQIVLPLVRPMIAVQSLWAFMGPFGDF
                     MLAKFLLRAQENYTVAVGLQSFITNDARNPKVTLFAAGAILIAVPISVLFFFLQKNFV
                     SGLTSGGTKG"
     gene            complement(6502..7809)
                     /locus_tag="VHI09_001010"
     CDS             complement(6502..7809)
                     /locus_tag="VHI09_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ6120894.1"
                     /translation="MVDNQRQQKPNKAMWLSVIPGLGQLYNKQIVKGGVLLVVFFLEL
                     LEIVVLGIPALTGLYSLGSVPMQDHSLFMLIKGAMQLITLVLFGIFHLVAMRDAKLVA
                     HQMNEGKKVPVTAKETLEAIYEKGFPYLLIIPAYLAMAFAIIFPVLVTLLIAFTNYDF
                     RHIPPYRLLDWVGLKNFLNILELSTFKTAFTSVLSWTLIWTLAATSLQIVIGIATAVI
                     ANQSFIRGKRLFGVIFLLPWAVPAFISIMSFGNFFNDSIGAMNVQVLPFIEKFLPFVD
                     FGIVSWKTDPFWTKVAVIMVQGWLGFPYIYILVSGILQAIPADLYEAATVDGATVLQK
                     FRHITMPMIFAVAAPTFISQYTFNFNNFSIIYLFNNGGPGTVGGGAGTTDILISWIYK
                     LTTQTSPQFSMASAVTLIISVIVISVSLITFKKFKAFDMEERS"
     gene            complement(7884..10019)
                     /locus_tag="VHI09_001011"
     CDS             complement(7884..10019)
                     /locus_tag="VHI09_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrin glucanotransferase"
                     /protein_id="HEQ6120895.1"
                     /translation="MRELHIKTYKLLTKSAVLLGLISFPLTVSAADNASVTNKADFST
                     DTIYQIVTDRFNDGNTSNNGKTDVFDKNDLKKYHGGDWQGIIAKIKDGYLTDMGISAI
                     WISSPVENIDSIDPSNGSAAYHGYWAKDFFKTNQHFGTEADFQQLVKVAHQHHIKVVI
                     DFAPNHTSTAEKEGTTFKEDGALYKNGKLVGKFSDDKDKIFNHESWTDFSTYENSIYH
                     SMYGLADLNNINPKVDQYMKEAIDKWLDLGVDGIRVDAVKHMSQGWQKNWLSHIYEKH
                     NVFVFGEWFSGHTDDDYDMTTFANNSGMGLLDFRFANAIRQLYTGFSTFTMRDFYKVL
                     ENRDQVTNEVTDQVTFIDNHDMERFATKVANNQTAVNQAYALLLTSRGVPNIYYGTEQ
                     YATGDKDPNNRGDMPSFNKESQAYKVISKLAPLRKQNQALAYGTTEQRWISDHVLVFE
                     RKFGNHVALVAINRDQTNGYTITNAKTALPQNSYKDKLEGLLGGQELIVGADGTISSF
                     ELGAGQVAVWTYEGEDKTPQLGDVDASVGIAGNKITISGQGFGNSKGQVTFGEISAEI
                     LSWSDTLITLKVPTVPANYYNISVTTADKQTSNSYQAFEVLTDKQIPVRLLINDFKTV
                     PGEQLYLMGDVFEMGANDAKNAVGPLFNNTQTIAKYPNWFFDTHLPINKEIAVKLVKK
                     DSIGNVLWTSPETYSIKTGHEAQTITIKK"
     gene            complement(10045..11748)
                     /locus_tag="VHI09_001012"
     CDS             complement(10045..11748)
                     /locus_tag="VHI09_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEQ6120896.1"
                     /translation="MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGP
                     KYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTT
                     SYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGW
                     LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQF
                     GTKYDLQELIDLAHQMGIKIILDAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEH
                     PSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHD
                     FWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTS
                     KQLAEKLSNILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGI
                     PCLYYGTEVAMEGGYDPDSRRGFPWDDAERDKDFFSKVKAIIALRQQPALQEGAISID
                     YDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQDGYIVFS
                     N"
     gene            complement(11914..13173)
                     /locus_tag="VHI09_001013"
     CDS             complement(11914..13173)
                     /locus_tag="VHI09_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ6120897.1"
                     /translation="MSWNWKKTSVLGTLSLASVLPLTACVSGGGKGVKETDGKTIVVS
                     VDEGYVDYIKSIKGEFEKEHKVTVKVKKEGMMDTLDKLSTDGPTGASPDVFLAPFDRV
                     GGLGTEGQIAEVTLGNSKEFDDTVKKLVTIDGKTYGAPDVIETLVTYYNKDLVPQAPK
                     SFTELEVLQKDSKFAFASEPGKSVGFLAKWTDFYYGYGLIAGYGGYIFGDKGTKPSDL
                     GLGNDGTVEGLNYAKQWYGTWPQGMQDTKKAGDFITEQFISKKAGVIIDGPWAASSFK
                     DAGVNFGVMEIPTLTNGKKYQPFAGGKAWVISNYSKGKTTAQKFLDYVTNAENQKRFY
                     DKTQEIPANLTARNYASKEGNELTKAVISQFESAQPMPNIPEMAEVWEPGANMFFNVA
                     SGKEEASKAAKEAAKTIKEAIEQKYAE"
BASE COUNT         4137 a         2754 c         2243 g         4124 t
ORIGIN      
        1 tcttaggagg aaatagctat gaaatcatgg caaaaagtta tcgtcggcgg agcaagtttg
       61 acacttgcaa gtaccttgtt agtgggatgt ggatcaggtt caaaagataa aaaggaagct
      121 ggcgctgact caaagactat taaactttgg gtaccgactg gctctaaaaa atcatatgca
      181 gatatcattg caaaatttga aaaagattct ggttacaccg ttaaagtggt tgaatcagaa
      241 gatccaaaag cacaagaaaa aatcaaaaaa gatgcctcaa ctgctgccga tgtcttttca
      301 cttcctcatg atcaacttgg tcaacttgta gaatctggta ctattcaaga agttccagaa
      361 aagtacaata aagaaattgc tgctacttct actgatcagg cgctcgtcgg tgcacagtac
      421 aaagggaaaa catatgcttt cccatttggt attgaatcac aagttctttt ctacaataaa
      481 tcaaaattaa ctgctgaaga cgttacttca tatgatacaa ttactacaaa agcaactttt
      541 ggtggtacct ttaaacaagc taacacttac gctactggtc cattattcat gtcagttggt
      601 aacactttat ttggggaaaa cggtgaagat gtaaaaggta ctaactgggg caatgaaaaa
      661 ggcgctgctg ttcttaaatg gatcgcagac caagcttcaa ataaaggttt tgttagctta
      721 gatgctaaca acgttatgtc taaatttggt gacggttcag tagcttcttt tgaatcagga
      781 ccgtgggact atgaagctgc tcaaaaagct atcggtaaag aaaaccttgg tgttgctatt
      841 tatccaaaag taactattgg aggcgaaacg gttcaacaaa aagcattctt gggtgtaaaa
      901 ctttacgcag tcaaccaagc accagctaaa ggtgatacaa aacgtatcgc agctagctac
      961 aaattggcat cctacttgac taatgctgaa agccaagaaa accaattcaa aactcgtaac
     1021 attgtcccag ccaacaaaga agttcaatct tcagaagctg ttcaatcaaa cgaacttgct
     1081 aaaaccgtta tcactatggg ttcttcttca gactacactg tcgtaatgcc aaaacttagc
     1141 caaatgggca cattctggac tgaaagtgct gctattctta gtgatgcctt caacggtaaa
     1201 atcaaagaaa gcgactacct tactaagttg caacaattcg acaaagatat cgctgcaaca
     1261 aaataatatg atctcatgtc atcactgcta tactaaaaca agcacaaggt aagtacttct
     1321 aaaaagttct ttccacttac cttatgaaga gtggaactag gctccatcac attttattca
     1381 agcttagtct cactcttact tgtttttgtt ttactagtag cttagtgcta cttaaatttt
     1441 taactcttta aaagtttacg taacacttag ctatttcatc ctaacaagag attgaatctg
     1501 aactaccact ctagtatcct aaaataaagg agaatctata tgactcagtc aaacttgaca
     1561 ccaaatatat ctgtaataga agctttaaaa agaggcagtt gggatattaa actttcgagt
     1621 attatcatgg gatttgccaa ctttgctaac aaacagttta tcaaaggaat cttattttta
     1681 ataagtgaac ttatctttct agtagctttt gtctcccaaa tcatccctgc tatccgtggc
     1741 cttgtcactc ttggtactca aactcaggga atgactacaa aaactattga tgggattaac
     1801 atccaagttg ctgttgatgg cgataactct atgttaatgc ttatttttgg tttagcatca
     1861 ctcattttct gcctagtctt tgcttacatt tactggtgta atctcaaaag tgctcgtaac
     1921 ctttatcttt tcaaacaaaa aggtcagaag ataccaagct tcaaagaaga tcttgccaca
     1981 cttacaaatg gtcgctttca tatgacttta atggctatcc cactgattgg tgtgctttta
     2041 ttcaccattc ttcctttgat ttacatgatc tgtttggcct ttaccaattt tgatcataat
     2101 cacttgcctc caaaatcttt atttgattgg gtgggacttg ctaactttgg aaatgtttta
     2161 agcggccgta tggcaggaac cttttttcct atcttctcat ggactttgat ttgggctgtt
     2221 tttgccacag taactaattt cttctttggt attattcttg ctttgttaat caataccaaa
     2281 ggattaaagt ggaaaaaaat gtggcgaacc atctttgtta tcaccattgc agttccacaa
     2341 ttcatctcat tattgattat gcggaactta ctcaatgacg aaggcccact aaatgctctt
     2401 ctcaataaaa tcggcttgat taatggctca ctgccatttt tatctgatcc tctttgggca
     2461 aaattctcaa tcatttttgt caacatgtgg atcggtattc catttaccat gctgattgcg
     2521 actggtatta tcatgaacct tccaagtgaa caaattgagg ctgctgaaat tgatggcgct
     2581 agcaaattcc aagtcttcaa gtctatcacg ttccctcaaa ttctcttgat catgacacca
     2641 aacttgattc aacaatttat cggaaatatc aataacttta acgtcattta cctccttact
     2701 ggtggtggtc caacaaattc agaatactat caggcaggaa caacagactt gctggtcact
     2761 tggctttata aattaaccgt cacagctgct gactacaatt tagcttctgt tatcggtatc
     2821 ttaatcttta cagtttcagc tatctttagc ttacttgctt atacaaggac agcatcctac
     2881 aaggaaggag cggctaaata atgaaaaaca aacgtcgatt tcaacttgga cttgtttatg
     2941 ctactttaat catcctctca atcatttggc tcttcccaat cgcttgggtt attcttacta
     3001 gcttccgtag cgaaggaact gcttacgtca attattttat tcctaagact tttacattaa
     3061 atcactacat taatcttttt actaatgaaa cctttccttt tggaaaatgg tttatgaata
     3121 ctcttattgt agccactttt acatgtatta tttcaacctt cataactgtt gccatagcct
     3181 actctcttag ccgaattaag tttaagtttc gcaatggctt tttaaaacta gcccttattt
     3241 taaatatgtt ccctggtttc atgagcatga ttgccatcta ctatatctta aaggccttag
     3301 gtttgacgca aaccctaaca gcacttgtcc ttgtttattc ctcaggtgca gcccttggtt
     3361 tttacattgc aaaaggattt tttgatacca ttccttattc acttgatgaa tcagctatga
     3421 ttgatggcgc tactcgtatg gatattttct ttaaaataac ccttccttta gcaaaaccaa
     3481 tcattgtcta tactgccctt cttgctttta tggggccttg gattgacttt atctttgcac
     3541 aagttatttt aggtgatgct acaagtaaat ataccgttgc tattggacta ttttcaatgc
     3601 ttcagccaga taccattaac aactggttta tggccttcac tgctggttct gtcttaattg
     3661 ccgtcccaat cactctcctc ttcatgttta tgcaaaaata ctatgttgaa ggcatcacag
     3721 gtggttctgt taaataagtt acaacatacc aaaccttcta agggcaagcc ctttaggagg
     3781 tttttgataa ataaggccgt agataatcat attagtcctc acatttatct tcagatcagc
     3841 caaatgtatt aatgattcct tgacttttac catgaattcg gatataacaa aatgatgaca
     3901 tctaaaatta ccattaaaga tattgcacga gaagccgggg tttctgtggc gaccgtatct
     3961 tacgtgttaa accagcgcaa tgatcaaaaa atcagcgaag aaacccgcaa taaagtgcta
     4021 catattagta accttcttaa ctatacacct aatcaggcag ccaggtcatt agtcacacaa
     4081 caatcaaaca gtgttgtcct gtattacaaa tatgcagata gcattttagc tagggctgaa
     4141 caactgcata ccattagctt tctatcccgc ctattgcatg agcaggacta tcagctggtt
     4201 tgcctaaatg aagatagcat tgaaaaattt gactgggcag accttattct gaccttggac
     4261 gttgataatg atacctttta caaaattgga aagcttaatt tcaaaccctt aattgccatt
     4321 gatagctttg ttaatgatcc cctcttcttt caaattaata cagattatgc tgctctcaaa
     4381 gatcaggcta atcagttttt taaggatgaa ccttataccc tgctttcctt gcctatcact
     4441 aatcgtgagc gatatgagca tgtttctgcc ttctttccaa gtttgcacat tgtaaacgac
     4501 ctaagagagc taacctactt tgaaaaggaa aatcttttac tcattgatga gagtttggat
     4561 caattctttg tgaactcttc gcaaaaactc tactgcccac gtttttcccc caaaaaaggg
     4621 caagttatct tagaggccat cgaatgggcc aaaaaacgcc agcctgataa aaaacataac
     4681 cttctggtgt aacgcctgag aattaaccta tcttgactaa ttccttgtaa ctcaaaacct
     4741 tgcctcttta gataaagaag caaggttttt tgatgtctaa tctaataaca gggcctgact
     4801 tgtctcatca atctctaaac tgtgttttgt agaacaccca ggtcagcatt aataaggtac
     4861 caaatacttg gcaattcata atcagtactg gatcctgaat aaagaaacta agagcaatcg
     4921 ccagtagact cggccaagcc aaacaattaa ccatcaatcc aagtccttca gagaagtttc
     4981 gaatcataaa tagtcgtgat cgtttagtca gagtcaaaaa gaaagcgcct agaccaatca
     5041 caataagggt acccacacac accatgagaa caaacgtcag caaaagaaaa ccaagtacac
     5101 tggctttatt actgtcatac cattgctgat tgacaaagtc cttcactgct ttaactgttg
     5161 tcaggtcagc gagtgactgc tgctggcctc taacgtgagc acggagttcc ttgccatcag
     5221 gggtaacaaa acgccattct ttttttgtca caataatttg tctggttgat tctgatggca
     5281 attgctctaa atcgaccttt gtcggtaaaa cagccaaaac caattcacca tctcgtcgaa
     5341 tcagagattg accctgaaac gtatctttct caatctttcc ttgggtcaat gcttgatagg
     5401 ttttgtctgt gataggcgac aaactcttag ttacaatccg ttccaaggga tacgttgtca
     5461 tgttagcata gtgtaacgta actggaatca ttattaaggc gttcaaaaaa acaataataa
     5521 gaagattttg ccaccaagta aactgagctc ggctggcaaa cattgcccta ggagaaaggc
     5581 aatgtgaaat gtaagaaata ggaaattgtt ttttatctgg cattaaacat tactccttgt
     5641 caggttaccc tttggtacca ccacttgtta aaccggaaac gaaatttttc tgtaggaaga
     5701 aaaagagaac tgaaataggc actgcaatca gaatagctcc cgcagcaaat aaggttacct
     5761 tgggatttct agcatcattc gtaataaacg attgaagacc aactgctacc gtataatttt
     5821 cttgcgccct taaaaggaat ttagccaaca tgaagtctcc gaaaggaccc ataaaagccc
     5881 ataaggattg tactgcaatc attggtcgca caagaggaag gacaatttgg tagaaggtcc
     5941 taaagtgtcc agaaccatct aacttagcag attcgtccaa gtcataaggt accgtgtcaa
     6001 aataaccctt catcaaccag gcattcatag gaataccacc accgacgtaa atcaaaatca
     6061 agaaccagta ttggtttaag gcattaaaga gccacgccat aacaaagtaa gccgttaaag
     6121 ccgccatggt aggaaccatc tgtacgacta ggaaaaagat taaacttttt ttgcgtccaa
     6181 agaaattgta acggctatac gcataacctg tcaaagtgat gactgttact tgtatgagca
     6241 tggtgaagaa tgccacgatc aaggtattcc aataccatct taagtataag gtatcttgaa
     6301 agagtgtttt aaagttagac aaggtccatg gcccatcaaa atgaaaacta aaggctgtgg
     6361 tatttcctgg tctaaaagct gaactgaccg tcactagtat tgggaataaa atcacgatca
     6421 ctaaggagat tagatagaga taagtcaata attgggtgaa gaaacgtttg cgtttcatgg
     6481 atattgaaac ataggtcttt ctcatgatct ctcctccata tcaaaagctt taaatttctt
     6541 aaaggtaatc aacgataccg aaatcactat tacagagatg attaaggtca ctgccgacgc
     6601 catcgaaaat tgaggcgagg tttgtgtggt caacttgtaa atccaagaaa tcagaatatc
     6661 agttgtccca gctccgcccc ccacagtacc agggccccca ttattaaaca gataaataat
     6721 agagaaatta ttgaagttaa aggtgtactg agagataaag gttggtgctg caaccgcaaa
     6781 aatcattggc atggtgatat ggcggaattt ctgtaagact gtcgcaccat caacggttgc
     6841 tgcttcgtat aaatcagctg gaattgcctg taaaatacct gataccaaaa tatagatata
     6901 gggaaatcct agccaacctt gcaccataat aacagccact tttgtccaga aggggtctgt
     6961 cttccaggac acaattccga agtcaacgaa aggaaggaat ttctcgatga aaggaagaac
     7021 ctgaacattc atggccccaa tggaatcatt aaagaaatta ccaaaactca taatagagat
     7081 aaatgctgga acagcccatg gtaacaagaa tatcacacca aagagacgtt ttcctctgat
     7141 aaaggattga ttagcaataa cagccgttgc aattccaata acaatttgaa gacttgtcgc
     7201 tgctaatgtc cagatcagcg tccatgacaa aacagatgta aatgctgttt tgaaggtgct
     7261 taattctaag atattaagaa aattcttcaa ccctacccaa tccaataaac gatacggagg
     7321 aatatgccta aaatcgtaat tggtaaaagc aatcagcaag gtcactaaaa ctggaaaaat
     7381 aatagcgaaa gccattgcta aataagccgg aatgatgagc aagtaaggaa atcctttttc
     7441 ataaatcgct tcaagggtct ctttagccgt caccggaacc ttcttgcctt cattcatttg
     7501 atgtgcaact aatttagcat ctctcatagc aaccaaatga aaaataccaa ataagactag
     7561 agtaattaat tgcatggccc ccttaattaa cataaataag gagtgatctt gcattggaac
     7621 acttccaaga ctgtaaagac ctgttaaagc aggaattcct agaactacaa tttctagtag
     7681 ctctaggaag aaaacaacta ataagactcc ccctttaacg atttgtttat tgtatagttg
     7741 tcccaaacct gggataacag atagccacat ggctttatta ggcttctgtt gtctttggtt
     7801 atctaccatt ttttattcct cttctcataa atgatttaag gttatcctcc ttatcatact
     7861 aacttccttt taagctgact aatttatttt tttatagtaa tggtttgtgc ttcatgacct
     7921 gtctttatac tataagtctc aggacttgtc cataaaacat tcccaatact atctttttta
     7981 acaagtttga ctgctatttc tttattgatt ggtagatgag tatcaaagaa ccagtttggg
     8041 tacttggcaa tggtctgagt gttattaaat agaggaccaa cagcattctt agcgtcattt
     8101 gcccccatct caaaaacatc acccatgaga tatagttgtt cccctggtac tgtcttaaaa
     8161 tcattgatga gtaaacgaac aggaatttgt ttatcagtca atacttcaaa ggcttggtaa
     8221 ctattgctgg tttgcttatc ggcagttgtc actgaaatgt tataataatt tgctggaacc
     8281 gtcggtactt ttaaggtgat aagggtatct gaccaagaaa ggatctcagc agagatttct
     8341 ccaaaagtca cttgaccttt agaattacca aaaccttgac ctgaaatagt aatcttattt
     8401 ccagcaatac ccactgaagc atcgacatct ccaagttgtg gtgtcttgtc ctctccttca
     8461 taagtccata cagcgacttg ccccgctcca agttcaaagc tactaatagt gccatctgct
     8521 ccaactatta attcttgacc gccaagaaga ccttctaatt tgtccttgta gctattttgg
     8581 ggcaaggctg ttttagcatt agtaattgta taaccattcg tttgatctct attaatagcc
     8641 actagtgcga catgattacc aaatttacgc tcaaatacca aaacatgatc actaatccaa
     8701 cgttgttcag ttgttccata agctaaagct tgattttgtt ttcttaaagg agctagctta
     8761 ctaatcactt tataggcttg tgactcttta ttaaaacttg gcatatcacc acgattatta
     8821 ggatctttat cacctgttgc atactgctct gtaccataat aaatattagg cacacctcta
     8881 gatgttaaaa gcaaagcata ggcttgatta acagcagttt gattattagc cacttttgtt
     8941 gcgaagcgtt ccatatcatg attatcaata aaggtcacct ggtctgtcac ttcattagtc
     9001 acctgatctc tattttcaag aaccttgtaa aaatctcgca tggtaaacgt tgaaaaacct
     9061 gtatacaact gtctaatagc attggcaaat ctaaaatcta aaagccccat cccactattg
     9121 ttagcaaatg tcgtcatatc ataatcatcg tcggtatgtc ccgagaacca ttccccgaaa
     9181 acaaagacat tatgtttttc atagatatga ctcaaccaat ttttttgcca accttgtgac
     9241 atatgtttaa cagcgtcaac tcggatacca tcaacaccca ggtctaacca tttatcaata
     9301 gcttctttca tgtactggtc aactttcgga ttaatgttat ttaaatcagc tagtccgtac
     9361 attgaatgat aaatagaatt ttcataagta ctaaaatcgg tccaagattc atgattaaaa
     9421 atcttgtctt tatcatctga aaatttacca actaatttac cgtttttata taaagcgcca
     9481 tcttctttga aagttgtgcc ttctttttcg gctgtagacg tatgattagg agcaaaatca
     9541 ataactacct taatatggtg ttgatgagct actttgacta gttgttgaaa gtctgcttca
     9601 gtgccaaaat gctggtttgt tttaaagaag tccttagccc aatacccatg atatgcagca
     9661 cttccattag aaggatcaat actgtcgata ttttcaacag gagaagaaat ccaaatggca
     9721 gaaatcccca tatctgtcag gtaaccatcc ttaatcttgg cgatgattcc ttgccaatca
     9781 cctccatggt attttttaag gtcattttta tcaaaaacat cagtcttacc attattagag
     9841 gtattaccat cgttaaaacg atctgttaca atctgataaa tcgtatctgt tgaaaaatct
     9901 gctttgttgg tgacagaagc attatcggca gcagaaacag ttagtggaaa tgaaattaag
     9961 ccaagtaaaa cagcactttt cgttaataac ttataagttt tgatatgtaa ttctctcatc
    10021 gctttttcct tttaaaataa ccttttagtt actaaacaca atatatccat cctggagtaa
    10081 ttgttttttg tttttagtta ccccattgct tgtcaaaatc tgtcctcctt ccttaataga
    10141 ataagcactc tcaccattgt taagaacaag tgaaatgctt tgctcaccta agctgcgttt
    10201 cagaatgagg cagtcttcgt cataatcaat gctaatagct ccttcttgaa gagctggctg
    10261 ttggcgaaga gcaataatcg cttttacttt gctaaagaaa tccttgtccc gctcagcatc
    10321 atcccaagga aaaccgcgac gcgaatcagg gtcgtaaccc ccttccattg ccacttctgt
    10381 cccataatac aagcaaggaa tgcccataaa gataaagagc aatgctaaag cagctaacaa
    10441 tttatctttg gaacccttaa cttgagtgaa gaaacgatgc gtatcatgag aatccaaaag
    10501 attcaagttc atgcgattga cttgccaagt gtttcgcatc aaaatgttgc ttaatttctc
    10561 agccaattgt tttgatgtca tggtttcaaa agcaaagtaa tccaaacagg ctttggtgaa
    10621 agcataattc attatcccat catactgatc ccctgccaaa taaggatagg catcatgcca
    10681 gttctcccca attaaaatgg catctgcttt ttccgcttta accgcctgac ggaaacgtcg
    10741 ccaaaaatca tgagatactt catcagaaac atctaggcgc caaccatcga tacaaaattc
    10801 tttaatccag tatcgcccaa tctcaatcag atagtcttgc acgtctctat ttgaggtatt
    10861 ccatttgggc atatagttac atccagcaaa ggtttcatag tttactaggt ccatggatgg
    10921 gtgttcatca tgagtcataa accaatcaaa aaacttagac tctttaccgt atctcaagac
    10981 atcctgaaat tccacggcat cactgctggc atggttaaag acagcgtcta gaataatctt
    11041 gattcccatt tggtgagcta aatcaatcaa ttcttgcaaa tcatatttag ttccaaattg
    11101 tggatcaata gcgtagtaat cactaatatc atatttgtga ttactaatgg attgaaaaat
    11161 tggcgttaaa tagatgacag taatccctaa atctttgagg taatctaatt tttctgtaat
    11221 tccctttaaa tctccaccag caaaactttt aggagtcggt ttttctaacc aacccatgtt
    11281 gacataactg tcaccttttt cccaatcccc acggttaaag cgatcaacaa agatttgata
    11341 aaaaaccgcg tcttttgtcc aaggaatgac ttgatgaaca tcaacagcat tgatataagg
    11401 catttggaag aaattataaa acccatttga aaaatcgtaa ctagtagtta acccatcttc
    11461 actaaaataa taggtttgac cttcttcttc aattttaaaa atgtaggcca acctcacatc
    11521 gtctaatgtt aaagtagttt cataataagc atgcgtatgg tctgtatagg caactgacat
    11581 ttccaattct tcttggaatt catgataacc atatttaggc ccgtggacca aattaacagt
    11641 aatacctcta tcttctttgg ccacgcgtaa tcggatgaca agttcctttt gagatatggc
    11701 aaagcaatac ctcgaatcag gaatatggag cattccggca acattcattt ctacctccat
    11761 taataaagta ttgacgatta cttctttttc tttaaaacga aaaaatagca gagcgactca
    11821 tacttctata tttttagtat ctgtttcatc ttcatcaaac taacagaaat agaagctggg
    11881 cgtacttctt tatcccagcc tcccttcttt gccttattca gcgtattttt gttcaatagc
    11941 ttccttaata gtttttgctg cttcttttgc agctttagat gcttcttcct taccagaagc
    12001 cacgttaaag aacatatttg cacctggttc ccaaacttca gccatttctg gaatatttgg
    12061 cattggttgt gcgctctcaa actgactaat cacagctttc gttaactcat taccctcttt
    12121 agaagcatag ttacgtgctg ttaagttagc tggaatttct tgtgttttat catagaaacg
    12181 tttttggttt tcagcattgg tcacataatc aaggaatttt tgagcagtcg tttttccttt
    12241 agaatagttt gaaataaccc aagctttacc acctgcgaac ggttggtatt ttttaccatt
    12301 tgtcaaagta ggaatttcca ttaccccaaa gttaacacca gcatctttaa aggagctagc
    12361 tgcccatgga ccatcaataa tgacaccagc ttttttagaa ataaattgtt cagtgataaa
    12421 atcaccagct tttttagtgt cttgcattcc ttgaggccaa gtgccatacc actgtttcgc
    12481 gtagtttaac ccttcaacag ttccgtcgtt acctaggcct aaatcacttg gtttggttcc
    12541 tttatcacca aagatataac cgccataacc ggcaattaaa ccataaccat aatagaagtc
    12601 tgtccatttc gccaagaaac ctacagattt tcctggttca gaggcaaaag caaacttaga
    12661 atctttttgt aagacttcta attcagtaaa gcttttaggt gcttgaggca ctaaatcctt
    12721 attataataa gttaccaaag tttcaattac gtctggtgca ccataagttt taccatcaat
    12781 ggttactaat ttcttaaccg tatcatcaaa ctctttgctg tttcctaaag taacctcagc
    12841 gatttgacct tcagttccta agccacctac acggtcaaaa ggtgctaaaa agacatctgg
    12901 tgaagctcct gttggaccat ccgttgatag cttatcaaga gtatccatca taccttcttt
    12961 tttgacttta acggtgactt tatgttcttt ttcaaactca cccttaatac tcttaatgta
    13021 atcaacatat ccctcatcaa cagagactac aatagtcttt ccgtctgttt ccttaacgcc
    13081 tttaccacca ccacttacgc aagcagttag gggtagcact gaagctaagg ataaagttcc
    13141 aagcacagat gtttttttcc aattccatga cataacatgt tcctcccgaa atggtttttt
    13201 cttaaacaca aatcaaatca tctatctact ctcatcatat tagcaaataa aagataac
//
LOCUS       DAUELZ010000046        12569 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.047, whole genome shotgun sequence.
ACCESSION   DAUELZ010000046 DAUELZ010000000
VERSION     DAUELZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12569
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.047"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..568)
                     /locus_tag="VHI09_001014"
     CDS             complement(<1..568)
                     /locus_tag="VHI09_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ6120898.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEMIISAIITFVLIPTLSRLKR"
     gene            complement(626..1171)
                     /gene="coaC"
                     /locus_tag="VHI09_001015"
     CDS             complement(626..1171)
                     /gene="coaC"
                     /locus_tag="VHI09_001015"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEQ6120899.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILTTIDTIWKL"
     gene            complement(1164..1856)
                     /gene="coaB"
                     /locus_tag="VHI09_001016"
     CDS             complement(1164..1856)
                     /gene="coaB"
                     /locus_tag="VHI09_001016"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEQ6120900.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNERLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            2037..2888
                     /locus_tag="VHI09_001017"
     CDS             2037..2888
                     /locus_tag="VHI09_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEQ6120901.1"
                     /translation="MKGRIVITIRDLTSLPIFVYGDGNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKAFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            2885..4084
                     /locus_tag="VHI09_001018"
     CDS             2885..4084
                     /locus_tag="VHI09_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEQ6120902.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNIAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            4081..5079
                     /locus_tag="VHI09_001019"
     CDS             4081..5079
                     /locus_tag="VHI09_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEQ6120903.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEEAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            5121..5453
                     /locus_tag="VHI09_001020"
     CDS             5121..5453
                     /locus_tag="VHI09_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEQ6120904.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVEHNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            5446..6258
                     /locus_tag="VHI09_001021"
     CDS             5446..6258
                     /locus_tag="VHI09_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEQ6120905.1"
                     /translation="MPSSFDLLGEMIGLLQTEQLTSSWACPLPNALIKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWSASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGHHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEAAQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKALYDTYLQKENNCE"
     gene            6251..7132
                     /locus_tag="VHI09_001022"
     CDS             6251..7132
                     /locus_tag="VHI09_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEQ6120906.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILGTKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            7179..8198
                     /locus_tag="VHI09_001023"
     CDS             7179..8198
                     /locus_tag="VHI09_001023"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ6120907.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNATDLAPYLGAITAEELG
                     DLIFS"
     gene            8326..9996
                     /locus_tag="VHI09_001024"
     CDS             8326..9996
                     /locus_tag="VHI09_001024"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ6120908.1"
                     /translation="MKSDIEIAQSVALQPITDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLILVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGIALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYDGKAVQFGPKAKTQLKQFAEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            10081..11658
                     /gene="cls"
                     /locus_tag="VHI09_001025"
     CDS             10081..11658
                     /gene="cls"
                     /locus_tag="VHI09_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEQ6120909.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEV
                     SFTGGINLADEYINEVERFGHWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYL
                     SDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHA
                     LRFASERGVDIRIIMPGVPDKGVPYALAKTYYKALMSSGVKIYEYQPGFVHSKVFISD
                     NTKAVVGTINLDYRSLYHHFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLTQRP
                     WYQKLIGLLVRTIAPLL"
     gene            11998..12336
                     /locus_tag="VHI09_001026"
     CDS             11998..12336
                     /locus_tag="VHI09_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120910.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT         3898 a         2162 c         2615 g         3894 t
ORIGIN      
        1 acctttttag tcttgatagt gttggaataa gtacaaaggt aataatagct gagatgatca
       61 tctcaacaat agcgtttgat gagatgatgg ctgttagtag tgctttaata tttccatcaa
      121 aaactgtcgc gaaaaaaata aatatcccag ataatacaaa tatagtattt gttagagaac
      181 caatgatacc ggagactatt aaaccaaact gattaggaat caatttatag caataataag
      241 gggtaatacc aatcaaaata cgtgggataa tagcaattat taaagaagcg aaagttccat
      301 gatcgacaaa aggtgaaaag agataatttg taggcagtag aatgatggta tttgtaatga
      361 cacttataat ccccattaat cctcctaaaa tagcacctat ccttgggcca tataagacag
      421 aagcaataat aactgggata tggacaagcg ttggtttgat tggaataggc caaatattaa
      481 agacaagact gctcacaaaa tgaatgacta acatgatggc aaaaaagata gcaatacgag
      541 aaatatctgc tgatttacgg tgattcattt tactctccaa aaaatataat acttaattct
      601 aaaaaggtga tcagaaaaac gactgttaaa gtttccaaat cgtatcaata gtagttaaga
      661 taacatcaat atcagccaaa gcaccaggtc ctttatctcc acaggctaat aagctagatt
      721 ttggaggaat ttctgtaaac cctattgtcg ataagcgttt aatattttct tgagtaatag
      781 ggttttgata cattttggta ttcatggctg gggctattaa ttttggtgtg gtagctggta
      841 gggcaagagc aacacttgtt acaatattat cagcgaatcc atatgctaga tgagcaatag
      901 tattagcaga ggcaggtgca acaataaata aatctgtgcg ttttgctaat tcgatatggt
      961 tgataacttt tgggtcatgc tcatccataa catctaaatg aatagcattt tttgataaca
     1021 cttgcagggt taatggtgta ataaattgtg tagcagcctg agtcattatg atgtgaacat
     1081 catagccaat tttagttaat tgactcgtaa gatctgctgc tttataggca gatatactac
     1141 cagaaacagc taaggtaata tgtttagtca tgtttggtca tcctttcgta taataagtca
     1201 gcaattgcct ctttggtatc tgcagatgca acttcgttat tggatatcaa aagagccttg
     1261 tgcatacctg tttggatatc aactaaatca ttggcgagaa tataatcagc atggttttta
     1321 gctagacttg ctctggctac tttaattaac tcttcttgag gcacattaac tagaagttta
     1381 aagccgacta gtttaatatt aggattccac tgtttaacat aagagataac tttgggtgtt
     1441 tttttcaaaa aaagaacctg ataatcagat gcagatgaga ttttcggctc actgttatgt
     1501 tcacatagaa aacaattaag gttatcagct tgagagactt gttccaaatc agtcatgtag
     1561 actggagtat agtcagaaac agccatacta tgaattagaa tgtcatggtg aggaacctga
     1621 tctttgagtg cagccattaa gtcgttaact gtttccactt caataatcct taggcgctca
     1681 tttggcaggg gtttagtggc tgttttagta gtgactaggg taacgtcatg atgatattgt
     1741 aaaaatcgct ctgtgattaa ttttccaagt tgcccagttg agtgattagt gatacctcgt
     1801 actgcatcta taggttcagt tgttccacct gatgttatta ttaatttcat agtcatattt
     1861 tatcaaaaaa ggagctgata gtaaacggaa gataatgacg ccagacattg tcttccgttt
     1921 gtattaatat ggaatagtaa tagatttcta gtaattaaaa cgaagttctg gaaagcaaga
     1981 cactatagta gctaaaatct aaattttaag gtaaaatagg gcaagcggtt tcaagtgtga
     2041 aaggaagaat tgtgataact attagagatt taacgtcact gccgattttt gtttacggtg
     2101 atggaaataa aaaagttcca ggagctttga gtccttttgt gtggacagag gtgtttttaa
     2161 aaactatcaa tcaagaacct aatcaactga tcttacatat ttggccgatg acaagaacag
     2221 ttatattagg aatgttagat agacagcttc cctactttga gcttgctaaa acagagattg
     2281 gcaataatgg ttatgttcct gttactcgta atattggcgg tttggctgtt gtcgctgatg
     2341 atgggatttt gaatttttcg cttgttattc cagaccattt ttcggaatct atcagtatta
     2401 gtaatgctta tttaataatg gttgatgtca ttagagagag tttttcggat tattatcagc
     2461 gtattgaata tcacgaaatt aaaaattctt attgccctgg taattttgat cttagtattg
     2521 caggccgcaa gtttgcaggt atcgcccagc gtcgtatcaa aaagggaatt gttgtgtcta
     2581 tttacttgag cgtttgtggg gatcaagcag cgcgtggtca actcatcaag gctttttatg
     2641 aggctggaac acaaggagaa gttaccaagg ttaattaccc tcaaattgat ccagaatgca
     2701 tggcaactct ttcagaactc cttgagacgc cttttacagt ggcagaagtc cttgaacgtc
     2761 ttcgattgac cttgcgacag cttggctttt ccctcacgga aaaaagtcca gatcaagcct
     2821 tactgactaa ttttgatgct gtctatgaac ggatgcagct agaagtcgtt agaaaggaag
     2881 gcaaatgaaa gaaagattta gtcctttatt tgagcctttg actttgccaa atgggagtca
     2941 attggataat cgctttgttt tgtctcctat ggtgaccaac tcatccacaa aagacggtta
     3001 tgtcacccaa gatgatgtat cctatgcttt gagacgagca gcttctgccc ctctacaaat
     3061 tacaggagcg gcctatgtgg atccttatgg tcaattgttt gaatatggtt ttagtgttac
     3121 aaaagatgcc gatatttctg gcttgaaaga gctagctcaa gcgatgaagg caaagggagc
     3181 taaagctgtc ttacaactga cccacgctgg ccggtttgct agccatgcct tgactaaata
     3241 tggctttgtc tatgggccta gttacatgca gttgagatct ccccaacctc atgaagtcaa
     3301 acctttaaca ggtcagcaaa tagaggaatt gattgcagct tacgctcagg ccactcggcg
     3361 ggcaattcaa gcaggctttg atggcgtgga agtatcttcg gcgcagcgct tattgattca
     3421 aacgttcttt tcgacttttt cgaacaagag gacagatagt tatggttgtc aaacactttt
     3481 taatcgctct aagttaactc tagctgtttt gcaagctgtt caacaggtga ttaaacaaga
     3541 agctcctgat gggtttattt ttggatttcg tgctacacca gaagaaacta ggggaaatga
     3601 tattggttat agtattgacg aatttttgca gttgatggat tgggtactta atattgcaaa
     3661 acttgattat ttagcgatag caagttgggg ccgtcatgta tttcgaaata ctgttcgttc
     3721 tcctggccct tattatggaa ggcgagttaa tcaggtagtt agagattatt taagaaataa
     3781 gctacctgtt atggctacag ggggaatgaa cacacctgac aaagctattg aagcactagc
     3841 tcatgctgat tttattggag tatctacacc ttttgtcgtg gatccagaat ttgctcataa
     3901 aatcaaagaa gggtgcgaag agagtattca tttaaggatt cgacctgctg atttgaaaag
     3961 tttagcgatt cctcaggctt ctttcaaaga tattgtacct ttgatggact acggggaatc
     4021 attgcctaaa gaatcaagaa ctttatttcg tagtcttact cacaattata aggagattaa
     4081 atgaaagtca gtattcttga ttatggggtg attgataagg aaaaaacacc ccaagaagct
     4141 ttattagaaa cgcgctgctt agcacaagta gccgacaaac ttggttttca tcgtttttgg
     4201 gtagcagagc atcataatat ttatgccttt gcaataagca gtccagagct gttgatgatg
     4261 catttagcag atcataccaa acagattcgg ataggttcag gaggtattat gcctctacat
     4321 tatagtagct ttaagattgc agaatggata atgactttag aggccttgca ccctaatcgg
     4381 attgatttag gtattggcaa ctctttagga actacgttag ttcaaagagc tctatcaagt
     4441 atccattgca aagacagcta tagtcaggtt gtcacagaat tataccagta tcttaatcct
     4501 gatcatttgt caccactacc gatttttgtt aaccctaggg gtaatactta ccctcagatt
     4561 tggacgctaa gcaatagttt ggagacagca gaactggctg gtcaattagg tttaggctat
     4621 acctttggta ttttccccta tataccaaaa gatcctataa cagaagctaa aagagtatcg
     4681 gcacattata gaaaggcttt taggccttca aagctattaa aaatccccaa gttaatatta
     4741 gcagttttta ttgtcctttc tgataccgac gaggaagcag aagctttggc taaaccgttg
     4801 gacatttgga tgcttggtca gcaagatttt aatgaattta agacctaccc tgatgttgag
     4861 gaggctagaa actatcactt aacagagaaa caaagagaag ccattgcagc aaatcgtagt
     4921 cgcatggtta ttggatcacc tcacacagtc aagaaacaat tggataggct gatagaagct
     4981 tgtcaagctg acgaattatt agccatcccc ttagttcctg aatttgccaa tagacaacga
     5041 accttagagc tgctagcaga cttatacgtg accatttaac cttaccaaaa acaattccct
     5101 aaactgaaag gaaagaaaca atgaaaaaaa ttgcaaatta cttattaatt gagaaaacag
     5161 atgaccgcta taccattagc atgacaccag aattacaaga cgatattggt actattggtt
     5221 acgctgaatt tacggataac gaccaccttg ccgttgatga cattatctta aatctagagg
     5281 cttctaagac cgtcatgtct gtcttgtcac cattggctgg ggcagttgtt gagcacaatg
     5341 aggcagccac actaacacca acccttttga attcagaaaa agctgaggaa aattggattg
     5401 ttgtgctaac agatgttgat caagcagctt ttgatgcctt agaagatgcc tagttcattt
     5461 gatttattag gggaaatgat tggtctgttg caaacagagc agcttactag ttcttgggcc
     5521 tgtcccttgc ctaatgcttt aataaagcga caagacttgt ggcgggcgct cattaaccag
     5581 cgccctgctt tgcctttatc aaaagattac ttgaatctgg aagatgctta tctagatgat
     5641 tggagtgctt cttttgttcc tgtatcagtc aaggactgcc aaaaaactaa ttataccagt
     5701 ctttttcttt accatgggga tattaggtac cttgctgtgg atgctattgt caatgctgct
     5761 aatagtgagt tattgggatg ttttattcct aaccatggct gtattgataa tgcgattcat
     5821 acgtttgccg gaagtcgttt acgcttagct tgtcaggcaa tcatgactga acaaggccgt
     5881 aaggaagcca ttggccaagc aaagcttacc agtgcttatc atctaccagc ctcttatatt
     5941 attcataccg tagggccaag aattaccaaa ggacaccacg tttctccgat tcgagctgat
     6001 ttgttagcac gttgttatcg ttctagtctt gatttggcgg ttaaagcagg attgactagc
     6061 ctagcttttt gctctatttc aacaggagaa tttggctttc ctaaaaaaga agccgctcag
     6121 attgctatta agacagtttt aaaatggcaa gcagaacatc cagaatctaa gaccttaact
     6181 atcattttta atacctttac atcagaagac aaggccttat acgacactta tttgcaaaag
     6241 gagaataact gtgagtaatt ggacaaccta tcctcagaaa aacctcaccc aggcagaaca
     6301 actggcccaa ttaatcaaag aagcagatgc acttgtagtt ggtattggag ctggcatgtc
     6361 tgcagctgat ggttttactt atatcgggcc gcgttttgag acggcattcc cagattttat
     6421 tgctaagtat cagtttttgg atatgctgca agctagcctt tttgattttg aagattggca
     6481 agaatattgg gcttttcaaa gtcgctttgt cgctttgaat tatcttgatc agcccgtagg
     6541 ccagtcttat cttgatttaa aagagatttt gggaaccaaa gactatcata ttattactac
     6601 taatgctgat aatgcttttt gggtggctgg ttatgatcct cataacattt ttcacattca
     6661 aggagaatat ggtttgtggc aatgtagcca gcactgccat cagcaaactt ataaggacga
     6721 tacggtcatc cgtcaaatga tcgcagagca aaaaaacatg aaagtcccag gtcaactgat
     6781 tcctcattgc cctgaatgtg aggcgccttt tgaaatcaat aagcgtaatg aagaaaaagg
     6841 aatggttgaa gatgctgact ttcatgccca aaaagcacgt tatgaggctt ttttatcaga
     6901 acataaagag ggtaaagtgc tttatctaga aattggtgtg ggacatacga cgcctcagtt
     6961 tatcaaacac cccttttgga aacgtgtctc agaaaaccct aatgccttat tcgttacctt
     7021 gaatcacaaa cattaccgca tccctctgtc aataaggcga caaagcctag aactgacaga
     7081 acacattgca caattgatta gcgccacaaa aaccatttac caaaaatcgt aaccaaagcc
     7141 aatagacggt taattaagag atagagaagg agactaacat gtaccttatt gaaccgattc
     7201 gtaacggtaa acgtatcacc gatggagctg ttgctctagc catgcaagtt tatgttcaag
     7261 aaaatctatt tttagatgat gatattttat ttccctacta ttgtgatccc aaagttgaaa
     7321 ttggaaaatt ccagaatgca gttgtagaaa ctaatcaaga gtatttaaaa gagcatcata
     7381 tccctgttgt tcgcagagat actggcggtg gtgccgttta tgtagattct ggtgctgtta
     7441 atatttgcta cttgattaat gataatggta tttttggtga ttttaaaaga acctatcagc
     7501 ccgctattga agcccttcat catttgggag caacagaggt tgaaatgtct ggacggaatg
     7561 acttggttat tgatgggaaa aaggtatctg gcgctgcgat gactattgct aatggccgtg
     7621 tttatggggg atattccctt ttgctagatg tggactttga agccatggaa aaagcattaa
     7681 aacctaatcg caaaaaaatt gaatcaaaag gcatcaggtc agttcgtagt cgcgttggaa
     7741 acatcagaga acatttagca ccacaatacc aaggtattac gattgaagag ttcaaagatt
     7801 taatggtttg ccaattgtta cagattgaga cgattagcca agctaagcgt tatgatttaa
     7861 cggaaaaaga ctggcaacaa attgatgctt taacagagcg taaatatcac aattgggaat
     7921 ggaattatgg caacgcgcct caataccgtt accatagaga tggccgtttt accggtggaa
     7981 cagtagatat tcatttagat attaaaaaag gctatattgc agcttgtcgc atttacggag
     8041 atttttttgg caaggcagac attgcagaac ttgaaggtca cttgatagga acacgaatgg
     8101 aaaaagagga tgtcttagct accctcaatg caactgattt agccccttat ttaggtgcga
     8161 ttacagcaga agaattagga gatttgatat tttcttgata aaaaagatac ctcagactgg
     8221 taaagcacga ttaaaaacga acaataattg ttttaattca aataaatgta cgcatgatat
     8281 ggattgtgat ataatgacgt cattacgtaa aattgaggag tcatcatgaa atcagatatc
     8341 gaaattgccc aaagtgtcgc cttacaacca atcactgaca ttgttaaaaa ggttggaata
     8401 gacggcgatg acattgagct ttatggcaaa tataaggcta aattgtcttt tgagaaaatg
     8461 aaagctgtcg aagctaatga gcctggaaaa ttaatcttgg taactgctat taaccctact
     8521 ccagcaggag aaggaaaatc aaccatgtct attggactgg cagatgcctt aaatcaaatg
     8581 ggaaaaaaaa ccatgcttgc cttacgtgaa ccctcccttg gtcctgttat ggggattaaa
     8641 ggcggggcag caggtggtgg ttatgcccaa gtattgccta tggaagacat taatcttcac
     8701 tttactggtg acatgcatgc cattacaacc gctaataatg cattatctgc tttgattgac
     8761 aatcatttgc agcaaggaaa cgatttagga attgatcctc gccgcattat ttggaagcgc
     8821 gtgttggacc ttaatgatcg cgctcttcgt caggtaattg ttggcttagg tagtccagtg
     8881 aatggtgtgc cacgcgaaga tgggtttgat atcacagtag cttctgaaat catggcaatt
     8941 ttatgtttgg caactgactt aaaagatcta aagaaacgtc ttgctgatat cgtcgtggct
     9001 tatacctatg atcgtaagcc tgtctatgtg cgtgacttga aggttgaagg agcattgaca
     9061 ttaattctaa aagatgctat caagccaaat ttggtgcaga ctatttatgg aacaccagct
     9121 cttattcatg gggggccatt tgctaatatt gctcatggtt gtaattctgt ccttgctacc
     9181 tcaacagctt tgcgattagc agattacact gttaccgaag caggattcgg ggccgaccta
     9241 ggagctgaaa aattcctaaa tatcaaagta cctaatcttc ctaaagcacc agatgctatc
     9301 gttattgttg caaccttgcg cgctcttaaa atgcatggag gtgtcgccaa atcagattta
     9361 gccgctgaaa attgtgaggc tgttcgctta ggatttgcca acttaaaacg tcacgtggaa
     9421 aacatgcgcc aattcaaagt tccagttgtg gtggctatca atgaatttgt agcagatacc
     9481 gaggctgaaa ttgctacctt aaaagcgctt tgtgaagaga ttaaggttcc agtagaatta
     9541 gccagtgttt gggcaaatgg agctgaggga ggcatagcat tagctaaaac agtggttcgt
     9601 gtgattgatc aagaagctgc tgactacaaa cgtctctatt cagatgagga tactttggaa
     9661 gaaaaagtga tcaatatcgt tacccagatt tatgatggca aagctgttca atttggcccc
     9721 aaggccaaaa cacaattgaa gcagttcgca gaattcggtt gggataaatt gcctgtttgt
     9781 atggctaaga cccaatacag tttttcagat aacccatcct tgcttggagc gccaaccgat
     9841 tttgacatta ccattcgtga atttgttcct aaaacaggag caggttttat tgttggttta
     9901 acaggtgatg tgatgaccat gcctggtttg cctaaagttc cagctgccat ggcaatggac
     9961 gtagcagaaa atggcacagc cctcggtctc ttttaggaaa aattgctcga ataattcgag
    10021 caagcgctcc tttcttcccg ctttactgtg atataataag gtaaatgaga ttttggaggg
    10081 atgattatca aaaagaaagc aaaagtaaaa tatttgctcc ataaagggaa acatggattt
    10141 ttaagaggaa tttttagtag aacaacaatc attgtgttat taattatttt gcaattagtt
    10201 ttcttatttc aatcttatgc ctggatggag cagtaccgtg tttggataac gattcttgaa
    10261 agtgtctttg ctattactat tgttttatat ttagttaata gtgatatgga tgctatttca
    10321 agaatgacgt ggttaatttt gattatgatt gctccattac tagggtcact gtttttaatt
    10381 tatacaaaat tggattgggg ttacagaggg ctaaaacaga gaataaatca tcttgtagat
    10441 ctgtctgcac cttaccttag cgatgatgac gctatattag aggtattaaa ggatagcaca
    10501 tcaaccacat atcatttagt acagtactta gaaagaagtc gtggcaactt tccgatttat
    10561 aataatacaa gggtaactta cttcccaaca ggtgaaactt tttttgatag tttaaaagaa
    10621 cagttattct tagctaaaaa gtacattttt cttgaatttt ttattattgc agaaggccaa
    10681 atgtggggag aaatccttag tattctagaa aaaaaagtca gtgaaggtgt agaagttagg
    10741 gtgttgtttg atggcatgaa cgaactatca acgctatcat cagattacgc caagagatta
    10801 gaacaaatag ggattaaagc taaatcattt ttaccgattt caccctttat ctctacctat
    10861 tacaattatc gagatcaccg aaaaattgtc gttattgatg gggaggtatc attcactgga
    10921 ggtattaatc tagcagatga gtacattaat gaagtagagc gttttggcca ctggaaagat
    10981 gctggtttaa tgcttgaggg tgaagcaaca gacagctttt taattttgtt tttacaaatg
    11041 tggtctatca cagaaaaaga actgattatt gatccttatc tttcagatca ttctttaaaa
    11101 cttccttcag atggctatgt tattccctac ggtgattccc cgcttgatac tgataaaata
    11161 ggtaaaaatg tttatataga cattttaaat catgctaaag agtacgttta tatcatgaca
    11221 ccttacctta ttttagatag cgagatggaa cacgctttac ggtttgcatc agaacgtgga
    11281 gtagatatcc gaattattat gccaggagtg ccagataagg gggtaccata tgccttagct
    11341 aaaacctatt ataaagcttt gatgtcttct ggagtaaaaa tttatgagta tcaaccaggg
    11401 ttcgttcatt caaaggtatt tatctctgat aatacaaaag cagttgtcgg aacaattaac
    11461 ttagactatc gtagccttta tcatcatttt gaatgcgcga cttatttata tcgtgtgtca
    11521 gttattgctg atattgtgaa tgactttaat gaagctcaaa agcaatcact attgatgaca
    11581 tcagatcatt tgacgcaacg tccttggtat caaaaattga taggattatt agtacgaaca
    11641 attgcccctc tcttgtaatt ttcgtaaaat atgttataat atctgtatgc gatgagtcga
    11701 ttgtgactgc aaagtcactt ttgcgcaggg gaggtcatta aggcgggaag ccgactttga
    11761 taatctgtgt gaaccatttt atcacaatct tatttattaa gaatgcccct tggattgaga
    11821 tattctcaat ccttttttta attttattat cttagttaaa attcagaatg tatcagtgat
    11881 tactgtgttt caaaataaga aagtagtttt tcgggtatga gtgctatgac ttgacaaatg
    11941 ctaataacat ttgcataatg taagtagaga tgaaaactta tgaaaagaga tgatcttatg
    12001 caaattatta agcgtgatgg ccacgttgct gattttgatc cagataaaat ttatcaagca
    12061 attattaaag cagcaaggac ggtctatgtt attgatgaca cgtggcgaca aaacttaaca
    12121 catgtcacta aaaaagtagt tattgattta gaagaagcac atgtagaacg tcctactatc
    12181 aatatggtcc aatcccttgt ggagaatcgt ttgatggatg ctggatatat taccattgca
    12241 gaacattaca tttcttatcg tcttcagcga gatttagagc gcaatggcta tggtgataag
    12301 attgtggttc acttgcgttt tgaacaaata aaataaaaac gcttctaatg gcattttagt
    12361 gtctttgagg ggataaggtt taaataccta acagataaaa atctgttagg tatttttttg
    12421 ctactattag gtgtattcta tattacatag aacttaagca ttcaagcttt aatttaactt
    12481 tctgtttatt ttttgctaca ttttgggaaa ctcccctcct ttcttagttg aggtgaataa
    12541 acttttattt gataaatcat atccttagt
//
LOCUS       DAUELZ010000047        12452 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.048, whole genome shotgun sequence.
ACCESSION   DAUELZ010000047 DAUELZ010000000
VERSION     DAUELZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.048"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            44..1387
                     /locus_tag="VHI09_001027"
     CDS             44..1387
                     /locus_tag="VHI09_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEQ6120911.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGRLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     GKQIAVLEIDEASLPKITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFICLNCGFKRPQLDYRLTELTTITNHSSEFIIDGQDYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIIETIEKQEAKHAYILATYTAMLEFRSLLADRHVVEKEMI"
     gene            1387..2178
                     /locus_tag="VHI09_001028"
     CDS             1387..2178
                     /locus_tag="VHI09_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEQ6120912.1"
                     /translation="MTYTSLKSPENQDYIYDLNIAHLYGNLMNTYGDNGNILMLKYVA
                     EKLGARVTVDIVSINDTFEQDDYDIVFFGGGQDYEQSIVAKDLPSKKAALADYIANNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTALKKKYGSAISLPSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(2287..3276)
                     /locus_tag="VHI09_001029"
     CDS             complement(2287..3276)
                     /locus_tag="VHI09_001029"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ6120913.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            3509..6115
                     /gene="hylA"
                     /locus_tag="VHI09_001030"
     CDS             3509..6115
                     /gene="hylA"
                     /locus_tag="VHI09_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEQ6120914.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQTLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQEPDHENRTYLWEHAKDYSASANITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWDYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFPPFEANSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSPIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNVFYNVYDNLKTYHDIKLMKELL
                     SDTSVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYCSYVNNQPVDLNNQLVDFTNTKSIFLESDDPAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYEKKATTFSNHNLSHQGFYSFPH
                     PVKQNQQQKLAHQGIAAKNNALNSHKIPHKRQRRLPRTGYQSSSLEFLGGALVASFNH
                     ITKPFRKKDLRI"
     gene            complement(6602..8365)
                     /gene="lpdA"
                     /locus_tag="VHI09_001031"
     CDS             complement(6602..8365)
                     /gene="lpdA"
                     /locus_tag="VHI09_001031"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEQ6120915.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAI
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            complement(8696..10105)
                     /locus_tag="VHI09_001032"
     CDS             complement(8696..10105)
                     /locus_tag="VHI09_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEQ6120916.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPTVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
                     RVHKEDVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLPEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            complement(10290..11288)
                     /locus_tag="VHI09_001033"
     CDS             complement(10290..11288)
                     /locus_tag="VHI09_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEQ6120917.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            complement(11347..12315)
                     /locus_tag="VHI09_001034"
     CDS             complement(11347..12315)
                     /locus_tag="VHI09_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEQ6120918.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
BASE COUNT         3780 a         2573 c         2233 g         3866 t
ORIGIN      
        1 ttttccctat ctaaaatcaa taaacttatg ataaaatata agcatgaaaa tgaagacatt
       61 actcggcatt attgctggca aagctgctca atccatttta acaaaactag ggcgaggatc
      121 tacttatcct ggtcgattgg ctctagcttg taataaagat attttaaagg atttgtctaa
      181 agactatgat attgtggtcg tcactggtac taatggtaaa acgttaacaa ctgctcttac
      241 tgtcggtatt ttgaaagagg catttggcga gataatcaca aatccaagtg gtgccaatat
      301 gattactgga attacttcta cttttttagc tgctaaaaaa ggaaaatctg gcaaacaaat
      361 tgctgtttta gaaattgacg aagctagtct tcctaaaatt accacttact taaaacctag
      421 tctttttgtt tatactaata tttttaggga tcagatggac cgctacggag agatttatac
      481 tacttatcag atgattgtag atggtgctcg aaatgctcca aaagcaacta ttctagccaa
      541 tggagatagt cctattttct cttctaagga catagttaat ccagttcagt attatggttt
      601 tgatactgct aagcatgctc cgcagctagc tcattacaac acagaaggga ttttatgtcc
      661 aaaatgcgaa catattcttc agtatcgtct caatacttat gcgaatctag gagactttat
      721 ttgtttaaat tgtggcttca agagaccaca attggattat cgattaactg aactcacgac
      781 gattaccaat cacagttctg agtttatcat tgatggccaa gattacaaaa tcaatgtcgg
      841 tggcttatat aatatttaca acgccctcgc tgccgtctct gttgctgaat ttttcggtgt
      901 ttctcccgag aaaatcaagg cgggctttaa caaaagtaag gcggtttttg gaagacaaga
      961 aacttttact attggtgata aatcatgcac ccttatcctt atcaagaatc ctgttggagc
     1021 cagtcaagcg cttgaaatga ttcagctagc tgattaccca tttagcttat ctgtgctttt
     1081 aaatgctaat tacgctgacg gcattgatac cagctggatt tgggatgcta attttgaact
     1141 catcacccaa atgcccatta cagaaattaa tgctggcggc gtccgccact ctgaaattgc
     1201 tagaagactt agagtcacag gatttgacga tactaaaata aagcaagctg aaaagctcga
     1261 gcaaatcata gaaaccattg aaaaacaaga agctaagcat gcctatattt tagcaaccta
     1321 tacggcaatg ttagaattcc gtagcttact tgctgaccgc catgtcgttg aaaaggagat
     1381 gatctaatga catacacttc actaaaatct cccgaaaatc aagactacat ctacgacctc
     1441 aatatcgccc atctctatgg taatcttatg aatacttatg gtgataacgg caatatttta
     1501 atgctcaagt atgtcgctga aaaacttgga gctagggtta ctgttgatat tgtgtctatc
     1561 aatgatacct ttgaacaaga cgattacgat attgttttct ttggtggtgg tcaagattat
     1621 gaacaaagca ttgttgccaa ggatttgcct agcaaaaaag ctgccttagc ggattacatt
     1681 gctaataata aagtggtgct tgccatctgt ggtggcttcc aattactagg gcaatactat
     1741 gtccaagcta atggggttaa aattgatgga cttggaatta tgggacacta cacccttaat
     1801 cagcaccaaa atcgctttat tggagatatt aaaattcata atgacgaatt taacgaaacc
     1861 tattatggct ttgaaaatca tcaaggacgt accttcttat ctggtgacga aaagccactt
     1921 ggacgagtgg tttatggcaa cggtaataat aaagaagacc aaactgaagg tgttcactat
     1981 aaaaacgtct atggtagtta tttccacggt cctatcttat cacgcaatgt taatcttgct
     2041 taccgcttag taacaacagc tcttaaaaaa aagtatggat cagctatttc actacctagc
     2101 tatgatgata tactgaaaca agaaatcact gaagaatatg ccgaccttaa aagcaaagct
     2161 tcctttaata aagtttaagt tcttctttca tgtcatcaaa caaaaactcc taagtaggac
     2221 acgatagttc gcttaggagt tttatagttt tttcttctga cagagcttct taagtgatta
     2281 aaaccactaa gaaacaatag ctttagcaac ttcttcaacg gtcatgcgtg agaagtactg
     2341 agtcgtatca atcgttttta aacgttcaaa aacatcacgg tactcatatt tacaaccaat
     2401 taataagttc tcaatgtctt gcacatcttt gattccaaag aaatcaccat agattttaag
     2461 gtttttaatg atagaatttt caacatttgc aaaggttgaa attttacctg cagggtaacg
     2521 aacattgcgt tcaatcgtat attctggcgc tttgccgtag gtccaatccc agctaccaaa
     2581 ttgctctttg gcagattgtt caatcttagc caactcatcc tccgacaaga catattctgt
     2641 catgtctggg tacgtttctt tcatcttggt caagatttta tctgaaaatt cctcaacggt
     2701 aattttttct ggtaattcat ttaaaatatt ggtcacacgg gcacgaacag acttcactcc
     2761 tttagactca atcttatcct tacttacctt aagagcatcg ccaagaacgg tcatatccac
     2821 atcaaacaaa aggcaaccat ggtgcatcat gcgacctttg tagtaagctt gagcattacc
     2881 acagattttt ttgccatcaa tttcaatatc attacggcct gtaaagttgg ctgttacgcc
     2941 caagtcagct aaggtcgcaa tcacaggttg tgagaaggtt ttgaaatcaa atgcgccttc
     3001 cgctgtttta tttgaaatga tagtgtagtt caagttattc aaatcatgat aaaccgcacc
     3061 accaccagat aaacgtcgca caacatgaat accatgttca tcaatgtatt ctttattgat
     3121 ttcttgaatg gtgttttgat gtttgccaat aataatagct ggctcattga tccaaaggat
     3181 aaacaattcg tcttcttcca ctagttctct aaaagcatag gcttccaaag cgatgttaaa
     3241 ggcaggattg tgacttttgt taacgatgta tttcatagga aaaaagtcct ttcaaaaata
     3301 tgtaatctac ttctctagta taatgaaaac aagcctaaat gacaagtgtc ctatctgtat
     3361 gacatcttca gtacctacac ttattagtgc acaaaaactc agctattatt tatgtaaggg
     3421 tttacaaaaa cactttaacg tggtatattt ttactgagta aaccagttta cttttattat
     3481 ctcattttta tatcggagga atttatcagt gaatacttat ttttgcacac accataaaca
     3541 attactactt tattcaaacc tattccttag ctttgctatg atgggccaag gaactgccat
     3601 ttatgccgat acactgactt caaattcaga acctaataat acttactttc aaacgcaaac
     3661 tctcactact acagatagcg aaaaaaaggt agtacagcca caacaaaaag actactatac
     3721 tgaattgtta gaccaatgga acagtattat cgcaggcaac gatgcttatg ataaaaccaa
     3781 tcctgacatg gtcacttttc ataataaagc tgaaaaggat gctcaaaaca ttattaaaag
     3841 ctatcaagag cctgaccacg aaaatagaac ttacctttgg gaacatgcaa aggattattc
     3901 cgcttctgct aatatcacga aaacttaccg caatattgaa aaaatagcaa aacagatcac
     3961 taatcctgaa tcatgctatt atcaagatag taaagctatt gctattgtaa aagacggtat
     4021 ggccttcatg tatgaacacg cttataatct agatcgtgaa aatcatcaaa caactggaaa
     4081 agaaaacaaa gaaaattggt gggattatga aattggaact cctcgtgcta ttaataatac
     4141 cttatccttg atgtatcctt attttactca agaagaaatt cttaaataca cagctccaat
     4201 cgaaaaattt gtgcctgacc ctactcgttt tagggttcgc gctgccaatt ttccaccttt
     4261 tgaagccaat agcggaaatt taattgatat gggacgtgtt aaactcattt ccggtattct
     4321 tcgtaaagat gatctcgaaa ttagtgatac aatcaaagca attgagaaag ttttcacgct
     4381 agttgatgaa ggaaatggtt tttaccaaga cggttcttta attgatcacg tggttactaa
     4441 tgctcaaagt ccactttata aaaaaggcat tgcttacact ggagcttacg gtaatgtgct
     4501 tatagatggc ttatcgcaat taattcctat tattcaaaaa acaaaatctc ctatagaagc
     4561 ggataaaatg gctactattt atcattggat taaccattct tttttcccta tcatcgttcg
     4621 tggagaaatg atggatatga ctcgagggcg ttctatcagt cgttttaatg ctcaatctca
     4681 tgttgctggc attgaagcac ttcgtgctat tttacgtatt gctgacatgt ctgaagagcc
     4741 tcaccgtttg gcacttaaaa cacgtataaa aacactcgtc acacaaggga atgtttttta
     4801 caatgtctat gataatttga aaacctatca cgatatcaaa cttatgaaag agctactaag
     4861 tgatacttct gttccagtcc aaaaacttga tagttacgta gctagtttca atagtatgga
     4921 taaattggca ctatataata ataaacacga ttttgctttt ggcctatcca tgttttcgaa
     4981 tcgaactcaa aattatgaag ctatgaataa tgaaaatctt catggctggt ttacttctga
     5041 tggaatgttt tacctataca ataacgattt aggacactac agtgaaaact attgggcaac
     5101 ggtaaatccc taccgcttac ctggaaccac agaaactgag caaaaaccac tagagggaac
     5161 tcctgagaat attaaaacga actatcaaca agttggcatg actagtctct ctgatgatgc
     5221 ttttgttgca agtaaaaaac ttaataatac aagtgctcta gctgctatga ccttcactaa
     5281 ttggaataaa agtctcactc tcaataaagg gtggtttatc ttaggaaaca aaataatctt
     5341 tgttggtagc aatatcaaaa accagtcatc tcacaaggcg tatacaacta ttgagcaacg
     5401 aaaagaaaat caaaagcacc cttactgttc ttatgttaac aatcaacccg ttgacttgaa
     5461 taatcagcta gttgatttta caaacactaa aagtattttc cttgaaagtg atgatcccgc
     5521 tcaaaatatt ggttactact tcttcaagcc aacaacactt agcataagta aggcgcttca
     5581 aacagggaaa tggcaaaaca taaaagctga tgacaaatca ccagaagcca tcaaagaagt
     5641 ttcaaatacc tttatcacta tcatgcaaaa ccatactcaa gatggcgatc gttatgccta
     5701 tatgatgctt ccaaatatga ctcgtcaaga atttgaaacc tatattagca agcttgatat
     5761 cgacttgtta gaaaacaatg acaaactggc cgctgtctac gatcatgata gtcaacagat
     5821 gcacgtcatt cactatgaaa aaaaagcaac gacgttttca aatcataatc tttctcatca
     5881 aggcttttat agttttcctc atcctgtcaa gcaaaatcag caacaaaagc ttgctcatca
     5941 aggaattgca gctaaaaaca atgctcttaa ctctcataaa attccccaca aaagacaacg
     6001 acgcttaccg cgtactgggt atcagtcctc ctcattagaa ttcttaggtg gagctttagt
     6061 tgcttctttt aaccatatca caaaaccctt tcgtaagaaa gacttaagga tttagctgtt
     6121 attggaaagt aggggttaac aatgaaacgt tattaaagac aaaaaagact ggaaactgca
     6181 ctgagcccca aaagttggct actctatttg aagtaaagga tttagttctg cattgtagag
     6241 gactaagtcc ttttcgtttt attttgatac gtttattgtt gcaataatcg atgataatag
     6301 taggtagaac gagtcaattt agccgtcctt aaaagaaaat ctaatcagaa ctcgtctttg
     6361 tccattcttt ataatggttt tttgtcttct tcttttcaga gatgcttctt tcaaacggag
     6421 ttctctcggt ttttttagcg ctaaatgtct agcttatctt catagcttaa tgtcatacaa
     6481 aaacacccca aacgttaggg ttttatatct aacgtttggg gtgcagttca tagttgtgct
     6541 agtctttttt gtgatctgtt atgtgatgat atttttcatt agttaataag acaaagacgt
     6601 tttacttacg ttttggtggg ttatggatag cttcgccaag cacatctgca aaggcttcgt
     6661 acatcacttc agagaaggtt gggtgtccat gaattgataa taacaattca tcaacggtta
     6721 attcagattc cataatagta gctgcttcgt taatcatctc agctgcagct ggaccaataa
     6781 tatgaacacc taagatttcg tggtatttgg catcagcaat aactttaaca aaaccatgtg
     6841 cttcatttga agcaatcgcg cgtccattac cagtaaagct gtttttacca ataagcacat
     6901 caccatattg ttcacgcgct tgttcttcag ttaaaccaac cattgccact tcagggtgtg
     6961 tgtaaacggc tgctggagtg tatttaaggt tagctttacg agttgtgttg ccatgtatcg
     7021 cattttctgc agccacttca cccatacggt aagcagcgtg agcaagcatt ttcgttccgt
     7081 taacgtcacc tggcgcatag atacctggaa ttgatgtctc ttggtagtca ttaactttga
     7141 tacggttacg atccatttca aggttaagat tttccagacc gttcatttgt ggtacacgac
     7201 cgatagaaag aagagctttt tcagcgacaa cttcttcacc attgttaagt ttcaaggtca
     7261 attggttatt tgcttcaaca atttcagaga caccaacaga tgttttgatc ttcatgcctt
     7321 tcttagaaag gattttttga agttcaagag agacttcttt atccatagct gggataatac
     7381 ggtcagccat ttcaataacg gtaacatcca caccgtaaga tgcccaaaca agtccaagct
     7441 cgataccaac aacaccaccg cccataactg ctagtgattt tggcatttca cggaggtcaa
     7501 ggatatcatc tgacgttaag acaagtttag agtcaatacc tgggatattg atacgtgata
     7561 ctttagaacc tgtagcaagg ataacgttgc gacccttaat ggtttgtgac ccaatggtta
     7621 cagtcttgtc agggttgact tgaccaagcc cattaaagat agtgacttta ttagctttta
     7681 gaagaccttg aacgccgcct gttaaggttt taacaacggt gtttttaaag tcaactgttt
     7741 tgtccatgtc aatggtatag ttagttgagg caaggttaat cccacgtcct gctgcaatct
     7801 taataccatc aaggatttca gcatttttaa ggtaagtttt ggttgggata caccctacgt
     7861 tcaagcaagt tccaccaaat tcagatttct caacgatggc aattttacca ccaagttgag
     7921 caccacgaat agctgcataa taaccagcag ggccaccacc aacaacgatg atgtcatact
     7981 catcatcagc aagtgcagct tttggtgcag ctgaagcagc ttgagctggt gcttttggca
     8041 cttcaagtcc agcagcttct aaatcttctg ttgtacgagc aacattcaca tctgaagcag
     8101 ctgggctaga aacctcaaca gattcacctt cagcaccgat gtagccgata acttctgtta
     8161 caggtactgt ttcaccagct tgacgtgtaa ttttaagaag aacacctgag tcttctgctt
     8221 caagttccat attggtttta tctgacatga tttcaagaag aatatcgcct tcattgacag
     8281 tatcaccttc ttgttttttc cactcgatga tttcaccttc ttgcatgtca acaccgagtt
     8341 ttggcataat aatttcaaca gccataattt aagataccaa gagcaaggag aaagcgattg
     8401 aatttttgcg gtaagtcaga aatctttgat ttcgttgacg cacccacgtc aggacgacta
     8461 ggactttccg aaacaaaaat tgaggaaagt tcagacgtct acgatgctaa gattagccaa
     8521 ctcttaccat ttttatccga gcttttcgct cgggttcaat tgcttatcac tcatttgcat
     8581 gagatctcct cgttctacat cctttctata tttttgagaa aagggcaccc accaagtcta
     8641 aacttagcaa aatccctttt attattgaat gcttttcttg tttaaaactg attgatcaga
     8701 ttaacaattc aaatggattt tccatcaatt tcttgagatc aaccatgaat tttgcaccgt
     8761 tcataccatc aactaaacgg tggtcgatgg ttaggcacat tgccataatt ggacgggcaa
     8821 caatctcacc atccacaacc gttggagttg ggatagttgc tccaacacca agaattgctg
     8881 agtttggttg gttaataatt gggttaaatg tcttcgtacc aaacattccc aagtttgtga
     8941 tagagaaggt tgatccagac atttcagctg ctttcaactt accagtttgc gcttttttaa
     9001 tcacatcttt tgaagcaagc acaaaatctg acaagctcat cttgtcagcc ccatggataa
     9061 caggaacgat aagcccatca tcaagaccta cggcaatacc aaggttcaca aagcggtgca
     9121 attcaatatc attagcatca ttgatgaggg aagcattcat gtactcatgc tcaggtttca
     9181 tcaaggtttt aaccactgcc ataccaatca agtctgtgaa gctgacttta aggcctgttt
     9241 tagccatgat tgggtcaatt aacttcttac gaagggcaat catctctgtc atgtcaatat
     9301 cgtagttaag cgtaaaggta ggggcagtaa ggtaagagtt tgtcatacct ttagagattg
     9361 ccttacgcat agctgacatt ggcttgtgtt ccacaccttc tggaagatca acaacttttt
     9421 cttctttagc tgctggggct tttgcttcag ctggtttagc cgcttcaaga atagccatga
     9481 tgtcttcttt catgacctta ccgttaaagc cagtacctga tacggttgcc aaatcaatac
     9541 ctttgtctgc tgcaatttta cgagcaagtg gtgaagcttt cggttgagca cctttaaagt
     9601 tttcaacgtc ttctttatgg acacgtcctt ttggtcctgt tcctggaact tggccaaggt
     9661 caatgcccat ttctgctgct gccttacgtg cagctggagt agcacgaact tttccgccat
     9721 ttccttgcgg aatagctgtc gctgccactt gaggagctgg acttgccaca ttttcttttg
     9781 gagcaactgt tggtccagca tctgctgaag caggaactgg aatttctgtt gttttttcac
     9841 ttgaagcaat agtatctact gattcaccct ctgcaccaat gtaaccgata acttcagtta
     9901 caggtacagt atcaccagct ggacggacaa ttttcaatag cacacctgag tcttctgcct
     9961 caagttccat attggttttg tctgacatga tttcaagaag gatatcacct tcattgacgg
    10021 tatcaccttc ttgttttttc cactcgatga tctcgccttc ttgcatgtca acgcccagct
    10081 ttggcataat aatttcaaat gccatttcta catcctttca cgatttgata agcgccatca
    10141 acatccccac cttgctacga aaacatacga aaggtgaggt gccgcgacac gggataagac
    10201 aactcagttc ttctaaaaac tttcaaaata gtttttagct gagacgttac tgataaacat
    10261 agcctttatc agaccacttg ccttatcttc tagttgcctt tgtttgccat tttcacaata
    10321 gcagctttta ttttctcaac gtctggtaag atcgcttgtt caagtacacg tgcataaggt
    10381 actggaacat cttcacttgc aagacgaacg attggatgat caaggtaatc aaaggcttca
    10441 ctttcggtaa tcatggttgc aatttcccca atgaatccac ctgttttgta agcatcattg
    10501 acaagcatca atttacctgt tttcttaaca gattcaataa tcaactcttt atcaagtggg
    10561 atgagggtac gtgggtcaac aacttcaacg ttaataccat ccgctgcaac ttcttcagcc
    10621 gcttgaagaa cacgctccaa catacgaccg tatgacacaa ttgtcaaatc agttccttca
    10681 cgtttgatgt cacctttgcc aagtgggata tagaagtcag gatcttggtt aacttcttct
    10741 tttttaccat aaagcgcttt tggttccatg aaaagaacaa tgttattatc acggatagct
    10801 gattttaata agcctttagc atcgttggca ttaccaggag caacaacttt gattcctgga
    10861 atgtgagtca accatgcttc aagagattgt gagtgttgcg cagcggaacc aataccagaa
    10921 cctgatgcca cacggaaggt tacaggggta ataaggcctc caccgaacat gtaattattt
    10981 ttagcaccat tattcacgat agcgtccatc atgatagtaa ggaaatccat gaaggtaacg
    11041 tcgacaattg gacgtagtcc tgtaatcgct gcaccaatag ctgcaccaga gatagcagct
    11101 tctgaaattg gggtatcttt aacacgttta ggaccaaatt cttcaatcat tccaacagaa
    11161 gtcccgaagt ctcctccgta aacccctacg tcttctccca taaggaaaat gttttcgtcc
    11221 ttgcgcattt cctcagtcat agcaaggttg actgcttcac gcaaagccat taattttgtt
    11281 tctgacatca tttttacttc tccatttatt attcaaatac gattgcttct tactgacaag
    11341 gtgatcttag tcaacccaaa cgtcctcgaa ggctactgaa agttctggat ctggactgtt
    11401 ttgtgcaaat tcataagcat cgtcaacttc ttttttgact tgtgcttgaa tagcatccaa
    11461 ttcgtcatca gtggcaatgc cttcgcttgt cagataagta cgatatttga tcattggatc
    11521 tttttctttc cactcatcta cttcttcttt agtacggtat ttaccagcat ctgcagtcga
    11581 gtgaccaaac caacggtatg attctacctc aacaatagct gggccatttc caccacgaac
    11641 atgctcaaca gctttgccca ttgtttcata gacagccatc acgtcattgc catcttcaca
    11701 gtagaaacca ggaattccgt aagcttctgc acgtgtgtat aagtgtggtg tatttgttgc
    11761 attattgatg ctcattgaaa ttccataacg gttgttgatg atgaagaaaa taacaggaag
    11821 tttccatgtt gcagccatat taacagactc gtggaatgat ccttcgttag tagcgccatc
    11881 acctgagaaa gcaacagcta tattgttagt tcctttgtat tgttgggtga gggcagcacc
    11941 aactgcaaga gcgtaaccac caccaacgat accattggta ccatagtttc ctttttcaaa
    12001 atcagccaag tgcattgagc caccacgccc tttagaaaca ccagttgctt taccagcaag
    12061 ctcagccatc attttattaa ggtccatgtc tttagcgatt gattgtccat gtccacggtg
    12121 atttgaaaaa ataatgtcat cgtaagacaa gtgagccacc gctccaacat tggctgcttc
    12181 ttcaccaact gagaagtgag tcatcccttg tacaaatcca cgacggacga gtttattaat
    12241 acgtgaatca aattcacgaa tacgttccat tttaaggaac atatccaaat gttgttcttt
    12301 agaaactgtt accatttcgg cctccatatt atcatattac ctttttatga taaaactttt
    12361 tttcacaatt tgcaaataat taaatcgctt tctaccttac cttatttggt tttttagaaa
    12421 tgcaatagtt tgtgaaatta caaacaaaac tg
//
LOCUS       DAUELZ010000048        12375 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.049, whole genome shotgun sequence.
ACCESSION   DAUELZ010000048 DAUELZ010000000
VERSION     DAUELZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12375
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.049"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(81..1340)
                     /locus_tag="VHI09_001035"
     CDS             complement(81..1340)
                     /locus_tag="VHI09_001035"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ6120919.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLLTNGQKIHGAHIYM
                     DTISVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
     gene            complement(1514..2710)
                     /locus_tag="VHI09_001036"
     CDS             complement(1514..2710)
                     /locus_tag="VHI09_001036"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ6120920.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKDVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVE
                     AVKTVL"
     gene            complement(3247..5559)
                     /locus_tag="VHI09_001037"
     CDS             complement(3247..5559)
                     /locus_tag="VHI09_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEQ6120921.1"
                     /translation="MKTKKVIILVGLLLSSQLTLIACQSRGNGTYPIKTKQSRKGMTS
                     NKIKPIKKSKKTNKTHKGVAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSLFEYLIPKGASLAKPAVAQQAASQGTSKVADPHHHYEFNPADIVAEDALDY
                     TIRHDDHFHYILKSSLSGQTQAQAKQVATRLAQTSSPVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAHVADQYDPAKKAEKPAETHHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPDPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTPFPSNEKDPNKM
                     KDWLATVIKLDLGSRKDPLQCKGLSLLPNLETLGLGFTPIKDISPVLQFKKLKQLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPVSHKESLTVVDLSRNADVDLATLQAPKLETLMVNDTKVSHL
                     DFLKNNPNLSNLSINRAQLQSLEGIEASSVIVRVEAEGNQIKSLVLKDKQGSLTFLDV
                     TGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPNKTVTNIDISHNNIPLADLKLNE
                     QHIPEAIAKNFPAVYEGSMVGNGTAEEKAAVATKAKESAQEASESHDYNHNHTYEDEE
                     GHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            5762..6703
                     /locus_tag="VHI09_001038"
     CDS             5762..6703
                     /locus_tag="VHI09_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEQ6120922.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYTDMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEK
                     RAIAIDLTDQGHLIVQFENGDLQTLRSGEISLSSWEN"
     gene            complement(6678..6881)
                     /locus_tag="VHI09_001039"
     CDS             complement(6678..6881)
                     /locus_tag="VHI09_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEQ6120923.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
     gene            complement(6976..8646)
                     /gene="dnaX"
                     /locus_tag="VHI09_001040"
     CDS             complement(6976..8646)
                     /gene="dnaX"
                     /locus_tag="VHI09_001040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEQ6120924.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISILALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAQKEQILSQANLSGELISEIETLKNELAQLKQQLSQLQSRPD
                     SLARSDKTKSKTTSYRVDRVTILKIMEETVRNSQQSRQYLDALKNAWNEILDNISAQD
                     RALLMGSEPVLANSENAILAFEAAFNAEQVMSRNNLNDMFGNIMSKAAGFSPNILAVP
                     RTDFQHIRKEFAQQMKSQKDSVQEEQEVALDIPEGFDFLLDKINTIDD"
     gene            complement(8646..9143)
                     /locus_tag="VHI09_001041"
     CDS             complement(8646..9143)
                     /locus_tag="VHI09_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEQ6120925.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFTGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKEGHLIGVLDLDSSLVADYDEVDQEYLETFVDLLLEKTTFTFNM
                     FGVKN"
     gene            9288..10097
                     /locus_tag="VHI09_001042"
     CDS             9288..10097
                     /locus_tag="VHI09_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEQ6120926.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     TEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            complement(10150..10413)
                     /locus_tag="VHI09_001043"
     CDS             complement(10150..10413)
                     /locus_tag="VHI09_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEQ6120927.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERNLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(10493..11119)
                     /gene="udk"
                     /locus_tag="VHI09_001044"
     CDS             complement(10493..11119)
                     /gene="udk"
                     /locus_tag="VHI09_001044"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEQ6120928.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            11217..12302
                     /locus_tag="VHI09_001045"
     CDS             11217..12302
                     /locus_tag="VHI09_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ6120929.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
BASE COUNT         3730 a         2604 c         2147 g         3894 t
ORIGIN      
        1 ataaaatgca acaaagtatg cacctaagta tcattagcct aagcacatgt aatataaaaa
       61 agagaagcgt tagcttctct ttagtcttcg attaattgaa tatctgcccc tagtgctgtt
      121 aattttgcaa tgatactagc atagccacgt aagataaatt caatattagt aatttctgtc
      181 ttgccttcag ccattagacc agcaatcact agagccgctc cggcacgtaa atcagtggct
      241 ttgacctgag ctcctgtcaa gcgagacggc ccttgataaa caatctgacc accaataact
      301 gagatatctg ctcccatgcg catcaactct ggcacatggt taatgcgctt ttcatatata
      361 gtgtcaataa tcgttccacg accatctgct tttaacaaca agggcgttaa tggctgttgc
      421 aaatcagtgg caaagccagg gtaaggagag gttttaattg taatagcctt gagggattct
      481 tgcttttcaa caaagatagc atcctcttct accgtcatac ggacacccat ttcttctaac
      541 ttagcaatga aactctctaa atgctcataa agaacattag tgatcttaac accttttcca
      601 atagctgccg ccaaagcgat ataagtccct gcttcaatac gatcaggaat aacttggtgt
      661 ctagtaccat gcaatttctg aacaccttgt atcgtaataa tatctgtacc tgctccacga
      721 atatgcgcac ccatgttatt caaaagagta gccacatcaa taatctctgg ctcacgtgca
      781 gcgttttcaa taaccgtttt accttgagct tttgttgccg caaccatagt attaattgta
      841 gcaccgacac taatggtgtc catatagata tgagcgccat gaattttttg accatttgtc
      901 aataaattca tattttcacc ctcataagac acttcaactc ccatagcctc gaaggccttc
      961 aaatgcaagt caattggtct tggtcctaaa tcgcaaccac caggtaagcc aacaactgct
     1021 tgtccaaaac gacctaaaag actaccgtag aaataatagg aagctcgaag actattaatc
     1081 tttccgtaag gcatcgggat gtcttgcacc cctctagggt caatttctaa agtatcacca
     1141 tgatagttta ctgttgctcc cataagctcc atgatttcaa tcagactatc tacatcacta
     1201 atagcaggta caccatcaag aatcacaata tcatcggcta agatgattgc tggaataagc
     1261 gcaacaacac tgttttttgc ccctgaaact gccacttctc cagataaggc ttttccgcca
     1321 ttaataataa ttttacgcat acattcaaaa ctttcttata ctagaattca accttttcat
     1381 tttatcataa atcatgaagc ctgacaatta ttgacgtctt aatttttaat catccctttt
     1441 aaaggcttat ttaataacaa aaaagcagag taagattttt tcactcactc tactttttta
     1501 ataaagttgt catttataat actgttttta cagcttctac taaagcatca accttattta
     1561 aacgctccca tggcaaatca atatctgttc tccccatgtg accataggca gctgtttgtt
     1621 tgtaaatcgg gcgttttaaa tcaagcattt gaataatgcc tgctggccgt aaatcaaaga
     1681 cttgacgaac agcagcttct aaaacagctt caggaaccgt tgaggttcca aatgtatcca
     1741 cgcgaacaga cacaggctga gccaccccaa tcgcataagc tagttggact tcagctttgg
     1801 tcgcaaggcc tgctgccaca aggtttttag caatatagcg agcagcgtaa gaagccgaac
     1861 ggtcaacttt tgtcgcatct tttcctgaaa aagcaccgcc accatgacgt gaatagcctc
     1921 cataggtatc cacaataatc ttacgtccgg tcaatccaga gtctccttgc ggacctccaa
     1981 taacaaaacg tcctgttgga ttaatgaaga actttgtgtc atcatctaaa taatctgctg
     2041 gaattacatc cttgatcact ttttcaatca catcctgacg aatttggtca ttagtagctt
     2101 ctggatcgtg ctgagtagaa ataacgactg tatccacacg aacaggttta tcgtgttcat
     2161 cgtactcaac tgtcacttga gatttagcat ctggacgtag ataactaatc tcacctgact
     2221 tacgtaattc cgccaagcga cgcactaatt ggtgagataa ggaaattggt aaaggcatca
     2281 attctggtgt ttcattgatg gcaaaaccaa acatgagccc ttggtcaccc gctccaatat
     2341 ggctaaggtc atcagtatca ccttctctgc tttcgagagc ttcattaacc ccctgagcga
     2401 tatctccaga ttgttctaca agtgatggat gtactcccac tgattctgct gaaaagccat
     2461 attctgcttc tgtataacca atttctgcaa tggtgtcgcg gaccacacgg ttaatatcaa
     2521 tgtatgctgt tgttgagatt tctccaaaaa catgaacaga acctgtgtaa acacaagttt
     2581 ctgcagccac atgggcttct ggatcttctg ctaaaatggc atctaaaata gcatctgaaa
     2641 tttggtctgc aatcttatct ggatgccctt cagatacgga ctcagacgtg aaaagtttac
     2701 gttctgacat aaaaatgtcc cccctaatat gttgattctt cctttttcag tcacattgac
     2761 cacaaagcaa gaagttgcct acccctttct tcctagctga ttaagcaagt caagtggcca
     2821 aaaccaactt agctaatcag ttacggtttt actagtaagg caactagcta gtccacctag
     2881 tggctagcgt ccattaacca gacaaagaat gtctgacaat agcgttgagt tacaaagaat
     2941 ccggtctcct tgttgagagg atgtcaccac aaaataagcc ttagacgaag taatttagct
     3001 aaagggagaa cgactgttcc ccaaataaag tcaacaacgt ctcaaaaaca tttcgttcga
     3061 cagaccgtct tatcgggact caataacaat ctattataac atgttttagc catttaggtg
     3121 ccattctttt cactcattat cctatttatt tcaaaaaaac ctcaaggtta ttcttgaggt
     3181 tttcttgata gtcaacaatg gtgtcattag gacgattcag ttggataggg ttattcttag
     3241 attagtttag tcagcatggt tttgctcatc tttagcttca ttttcatcct catgttcatg
     3301 gtcgtgatca tctttgtctc tgtgctcgtg agcatgacct tcttcatctt cataggtatg
     3361 attatggttg tagtcatgtg attccgatgc ttcttgagca ctttctttcg ccttagtagc
     3421 cacagctgct ttttcttcag ctgttccatt acctaccata gaaccttcgt aaaccgctgg
     3481 gaagtttttc gcaatggctt ctggaatatg ttgctcgttc aatttaaggt ctgccaatgg
     3541 gatattgtta tgactaatat caatgttagt aactgtctta ttgggtttag ataggttgac
     3601 atttgttaat tggtttttag acacgcttaa aatgtcaagt gctgtaaaat tattaacacc
     3661 ttctagagaa gtcaactggt tgcctgtcac atccaagaaa gtaagtgacc cttgcttgtc
     3721 tttaagcaca agcgatttaa tttggttacc ttctgcttct actctgacaa tgacgctact
     3781 tgcttcaata ccttcaagag attgcaattg cgcacggtta atagataggt tagatagatt
     3841 aggattattt ttcaagaaat ccaaatgaga aaccttggta tcattgacca ttaacgtttc
     3901 taatttgggt gcttgaagtg ttgctaagtc aacatcagca tttcttgata aatcaacaac
     3961 cgtcaatgat tctttatgag aaacagggct taaatcagaa attttattat tactcaatac
     4021 gaggaatttg agatttggta attgaccaag cggcctaata tcttcaatac cattatcagc
     4081 agccgctact agggttaagt ttttatattt gctcaagaaa ctaatatctt tgagattgtt
     4141 ttgtgaaata tcaatgcctt ctaactgtgg catattatcc aaaaatctat aatcagtcac
     4201 ccctgttttt gtcattaaca actgtttcaa ttttttaaat tgcaaaacag gtgagatatc
     4261 tttgattggt gtaaagccaa gtcctaaagt ttctaagttg ggtaacagtg aaagtccttt
     4321 acattgcaaa ggatctttac ggctgcccaa gtcaagtttg ataaccgttg ctaaccagtc
     4381 tttcatctta ttcggatctt tttcatttga tgggaatggg gttggagcat catgagtgcg
     4441 aacgatatcc aaaatcactt cttcatcaaa ccctaaggca cgcaaggtat ccattccaac
     4501 cttatgtttt tccaattcac gggcatgctc aatagcatgt ggatctggaa tgtcttttcc
     4561 gatttcaata tcagataaca tcaatacgtg tgcgtggtca tggtgagggt attccaaacc
     4621 aagtttaccg tcttgtgttt ccacacgttt aatagttgat ggatcaatcc ccaatttttc
     4681 tgccaaataa gctaattttt cttggtattc cttttcacgt tcagatagct ctggtgtgtg
     4741 atgggtttct gctggctttt ctgctttttt agcgggatcg tattgatctg ccacatgtgc
     4801 ccagccaccc tgacgaaggt ccgcaaaaga aataggatgt aagtgaccat cgtggtccac
     4861 taaaatactg tcttttgtta ccccaacaat accttgaccg ttaaattgaa aaccatctga
     4921 ggttgggaaa tgcaagcctg gaataccatt agctgtagct gttgaaacag ggctactggt
     4981 ttgtgccaag cgagtagcaa cctgtttagc ttgtgcctgt gtctgacctg ataagcttga
     5041 cttcaaaata taatggaagt gatcatcgtg gcgaatcgtg tagtctaaag catcttcagc
     5101 cacaatatcc gctgggttaa attcataatg gtgatgagga tctgctactt tagaagtccc
     5161 ttgactagct gcttgctgag caacagctgg cttagctaaa cttgctcctt ttggaataag
     5221 gtattcaaat agacttccct ttaaatcggc ataaaaaata aaatgcgaat gaccatcatg
     5281 gtcaacaacg attccctgat ctgtttttga taagattttt gagtctttgg ttaaaataaa
     5341 cccatcatct gtaggaaaat cgacacccgc cacacctttg tgagtcttgt ttgtcttttt
     5401 gcttttttta atcggtttaa ttttgtttga cgtcattccc ttacgtgatt gtttcgtttt
     5461 aatgggatat gtaccattac ctcgtgattg acaagctatc aaagtcaact gagatgataa
     5521 caatagacca actaaaataa taactttttt cgttttcatt caaccctcct ttttatttaa
     5581 ccagttaagt atatcgcatc acttgttttt cctcaagctc tttttaggat aactattaat
     5641 tgttgacgag ttggagctct gaagcttcaa caaacaatag tttctgttgt tagtagcatt
     5701 acacaaaaaa ctaacttttc ttttcctttg aaaataagat cttttataat atactataca
     5761 tatgaaaaca tcagaaaaaa tttatcaatt actgtcacaa accgatgact tcgtcagtgg
     5821 ggaatatcta gcagatcaac tcagtatctc tcgtacatcc gtttggaaat ccatcaaaag
     5881 tctcgaaaat caggggattc aaattgattc tttaaaacac aagggctacc gcatggtaca
     5941 aggtgatatt ttattaccca aaaccattag ccaagggctt gggatgccag tcacctatac
     6001 ccctcatagc caatctaccc aattagatgc caaacaagga atcgaagctc ataacagcgc
     6061 tccaaggctt tacctagctc ctagccaaga agctgccaaa ggacgcctcg atcggcaatt
     6121 tttttcagct agcaccggtg gtatttacat gtccatgtac ctaaaaccca atgtccctta
     6181 tactgatatg cctccttata cgatgatggt tgcttccagt attgtcaaag ctatctcgcg
     6241 attaacaggc attgatacag aaatcaaatg ggtaaacgac atctatctag gtaaccataa
     6301 agttgcaggg attctgacag aagccattac ttctgttgaa actggtttaa tcaccgatgt
     6361 cattattggg gttggtctca acttttttgt caccgacttc ccagaagcga ttgctcaaaa
     6421 ggcaggttct ctctttaccg aaaaaccgac tatcacgcgc aatgacctta tcattgacat
     6481 ctggaaatta ttcttatcta ttcctgtcaa ggaccatgtc aaagtttaca aagaaaaatc
     6541 acttgttctt aataaacaag tgacctttat agaaaatagt caagaaaaac gtgctattgc
     6601 gattgatctc acagatcaag gacacctcat tgtacagttt gaaaatggtg accttcaaac
     6661 cttgcgaagc ggtgaaatca gtctttcttc ttgggagaat tagccgtttt taaaatggtt
     6721 ttagtcagtt ggttaatggt atgcactctt cttaaatccc taaagagcaa caagccccaa
     6781 aataaggcga agagataatt tccagagagc aacaggtcat taaaaaaata ggtttcaaag
     6841 gcacatacaa aggccataaa aagaaattgt cgaatagtca tagctttatt ataacaaaaa
     6901 aatactgtac tatgaagaaa agtaacgaga taaaacgtgg gtaagggcaa aaccttaccc
     6961 ttatgtattt cagttttagt cgtcaatagt atttatttta tcgagcaaaa aatcaaaccc
     7021 ttctggaata tcaagcgcta cttcttgttc ttcttgaaca ctgtcttttt gcgatttcat
     7081 ttgctgagca aattccttac gaatatgctg aaaatctgtc cttggtactg ccagaatatt
     7141 gggagaaaaa ccagcagctt tactcataat gttaccaaac atatcattaa gattatttcg
     7201 gctcatgact tgttctgcat taaaggcagc ctcgaaagcc aaaatcgcat tctcactatt
     7261 tgctaagaca ggctcagagc ccatcaataa ggctctgtct tgggcagaaa tgttatctag
     7321 aatttcattc caagcatttt ttagagcatc tagatattgt cgagattgtt ggctatttcg
     7381 aaccgtttct tccatgattt tcaaaatggt aacccgatca accctgtagc ttgtggtttt
     7441 agatttagtt ttatcagatc ttgccagtga atcaggacgc gattggagct gcgacaattg
     7501 ttgtttaagt tgtgccaact catttttgag cgtttcaatc tctgagatta actctcctga
     7561 caagtttgct tgggacaaaa tctgctcttt ctgagctaat tggatggtca tcatttcggc
     7621 ataaatccga ggatgggttc cctttttgat ttcagggaga tgactagtaa caactgttat
     7681 catttggaat atacgatcta tcgagagaga caaattagta tcaaaaacag ctgactgacg
     7741 ctgattgtcg ccgccagctt taaccaccaa taaatcacgc agataggtca ataaatctgt
     7801 cgcaaagcgg ctcatgctct tcccactatc ataaatggtc tctaaggctg ccagagcttg
     7861 cgtagcctgt tcttgggaga catatcgaac atagtcacct agagcaagta tggaaataga
     7921 acctgtaatt tcttcggcaa ttgcaatggt gacctgatta tctggtgaca agctcaaagc
     7981 ctgatctaaa atagataaag catcacgcat gcctccttct gctcgccttg caatgagatt
     8041 taaagcatcc acctcatagg caataccttc tttgtccaaa acccaggcta aatgctctcg
     8101 aatagctttt tgcttaatag ctttgaattc aaagcgttgc acacgagata aaatagtggc
     8161 tggaatttta tgcaattccg ttgttgccaa gataaagaca acattttctg tcggttcttc
     8221 caaagttttc aaaagcgcat taaaagcccc tgttgataac atgtgaacct catcaataat
     8281 ataaaccttg taagtcgcac gactcggcgc ataggttgat ttgtctcgaa tgtcacgaat
     8341 ttcatcaaca ccattattcg aggcagcatc aatttcaatc acatcttcca agcttccatt
     8401 cgtgatatct cggcaaatat cgcattgatt acagggttca ccatcgactt ggttaggaca
     8461 attcatggcc tttgcaaaaa tctttgccgc acttgttttc ccagtccctc taggacctga
     8521 aaaaagataa gcatggctaa tcttgccaga ttcaactgcc tgctttaaag ttgtggaaat
     8581 aaccgattgt cccaccattt cgtcaaacgt ttggctccgg tatttccgat aaagagcttg
     8641 atacatcagt ttttaactcc aaacatgtta aaggtaaacg tggtcttttc aagaagtagg
     8701 tccacaaagg tctctaagta ttcttgatca acctcatcat aatcagctac aagactagaa
     8761 tccaaatcca gcaccccaat caaatggcct tctttcacca taggcacaac aatttcactc
     8821 atggctgccg catcacagga aatatagttg gcatgctgct tcacatcatt tataataatg
     8881 gttcgacgag actgagctga ttcgccacaa acacctttac caagcttgat atggacgcaa
     8941 gaaaccctac cttgaaaagg gccaagaatt aattcctgcc catcaaaaag gtaaaatccc
     9001 gtaaaaacag aattgggtag tgtcatgttt aacagggcgc tggcatttga caaattagcc
     9061 aaagcatttg attcattcgc aaatagctct tttgcctgcg ctatcattag ttggtattgc
     9121 tcaatttttt tagacttatt catacctttt attatagcat atctgggtct attcgagtat
     9181 tttttacgac tatctttcgc ttcagtcagt ttttaaaatg gtttttgagg ttgtttatga
     9241 tataattttc aatattatta atatcatctt aatattagga ggttttcgtg aaacaaatca
     9301 ctcaacagaa atggttacga tacggtcttt ttattgcttt agttttaaat ggtattgaac
     9361 ttgaattgtt aggactgact gctaacaacc tttcctttaa agaagctttt gctctaatac
     9421 taaccattag tctattaggc atttacttaa ttccatttgc agcagctatt ttttatttaa
     9481 gtaaaaaatt tcatatgaac ctgaacgtta ttattgttag ctgtcttagc ggactttata
     9541 tttcaggttt tttagcttct tgtgggaacc atctcgtagg acaattttgg tcatacatca
     9601 tcccttctaa agacgcttta aagctttggg gagatgcttt aactgcccct attgtcgaag
     9661 agcccattaa ggcttcttcg gctattttag taattacttt atttcctaga cttactctta
     9721 aagaaaaatt agttgttgct ttactttctg gcatgggctt tcaactaaca gaagatatcc
     9781 gttatttgat tcaagcaaaa tctatagata gtctcgtacc gactgctatt gaacgtatta
     9841 gcactgctgt gacttcacat tgggtacata cagctatttt taccattgga gcatacttat
     9901 tattaaaggg ttctaacctt ttttcaaaac agcaacaaat attctggtta ttaagtcctc
     9961 tggttctaca tttcatctgg aactctcctc taacaagcat tcctggtatg acagttttac
    10021 taggaacatt aatcctttta atctttggtg atctttttca aaaaatcaac acccttgacg
    10081 atgatgtgct tttttaaact ataaaagagc cctgactaaa taatactgat taagggtctt
    10141 ttaagtgact cacttcataa agatataagt tactagtaat gtcaacaaaa tactagtata
    10201 ccaagtgatg acaaagtacc actttctggt tttatggtgg tactttttag ctgcaagtaa
    10261 tgctccaaaa cctccaaaca aaactgttat cactaataag tttctttcac taatacgccg
    10321 tttctttttt atggcttttc ttttatcaaa ggcatatatg acaaacacga gttcattcca
    10381 aacaaatagt aatccaagcc aaattaataa catttctatt ttatgatccc tttctctagg
    10441 aagagcacaa caataaaaga gcagatttaa ataatctact cagccacttc tactatactt
    10501 ctccaagaat actcgcaatt ttactattaa taacatcaat agcaacgaca ttactaactc
    10561 cctctggaat aacaatatca gcatagcgtt tacttggctc aataaactga tgatacatag
    10621 gctttaccac gcttgtgtac tgatcaataa tactttctaa gcttctgcca cgttccatca
    10681 tgtcacgctt aatacgacga ataatacgaa tatcgtcatc tgtgtctaca aagagcttga
    10741 tatccatcaa atctcgcaaa cgctcatctt ctaggacaag aataccttca acaataataa
    10801 catcctgagg atcctgacga aaggtcgtat tactgcgggt atgctttttg taatcataga
    10861 taggaatatc tacaggtcta ccagctaata attcttttag ttgctgaatc ataaaatctg
    10921 tatcaaaagc tagaggatgg tcataattgg ttttgaccct ttcttcaaaa ctcatgtgag
    10981 actgatcttt atagtaagaa tcgtgttgaa tcatcgcaat acgagcattt ggaaaactgt
    11041 ccaaaatagc gcgtgacaca cttgttttac cgccacctga accaccagtt acaccaataa
    11101 taatcggttt tttaagcatt ttaaactcca aatctaaaaa gtctttctca ctattttacc
    11161 aaaattgaga ctataatgct ataatgaata tagaaacaat agtaaaagga agacgcatga
    11221 tcactaaatt tccccctcaa tggcaagaaa aacttgacca agttgcattt acccatttaa
    11281 cgccaatcca agaacaggcc tttcaaccta ttgttgatgg taaaaatttc cttggtatta
    11341 gccctactgg gacgggcaag accttagctt atgtctttcc tagtctattg gcactgacgc
    11401 ctaaaaagtc ccaacaacta ctcattttag cccctaatac cgaattagct ggccaaattt
    11461 ttgaagtcac aaaggattgg gcacagccac taggcttaac ggcacaactc tttatttctg
    11521 gaaccagtca aaaacgtcaa atcgaacgcc tcaaaaaagg tcctgaaatc ctgattggaa
    11581 cccctggacg catttttgaa ctgattaaat tgaaaaaaat caagatgatg tctgtcaaca
    11641 ccattgtttt agatgaatat gacgaattat taggtgattc ccaatatgat tttgttcaaa
    11701 aaattagcca ctatgttcct cgagatcatc aaatggtata catgagtgct actaataagg
    11761 tggatcaaac ttccttagct ccaaatacat tctgcatcga tctttcagag caaaccaacg
    11821 atgctattca gcatttttac ctcatggttg acaagcgcga acgtacagat ttattacgaa
    11881 aatttgctaa tattcctcat tttagagctt tggtcttttt caacagttta tctgatttgg
    11941 gtgctacgga agaacgtctc cagtataatg gagctgctgc ggtttcctta gcgagtgaca
    12001 ttaatgtcaa attccgtaaa accattctcg aaaaattcaa atcccaccaa ctttccttat
    12061 tgttagctac ggatcttgtt gctcggggca ttgatattga taatcttgac tatgtcattc
    12121 attttgatgt tgctcgtgat aaggaaaatt acactcacag agctggacga accggccgta
    12181 tgggaaaatc tggtatcgtt attacctttg ttagccaccc tgaagacctc aaaaaattga
    12241 aaaaatttgc taaagtctct gaaatctccc tcaaaaatca acaacttcat ttcatcaact
    12301 agttgttaaa aaacaaaact taatcaaggg ctactaaaaa acaacaatcc aattacttca
    12361 aaagtaattg gattg
//
LOCUS       DAUELZ010000049        11744 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.050, whole genome shotgun sequence.
ACCESSION   DAUELZ010000049 DAUELZ010000000
VERSION     DAUELZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.050"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(66..1685)
                     /locus_tag="VHI09_001046"
     CDS             complement(66..1685)
                     /locus_tag="VHI09_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ6120930.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            complement(1992..3536)
                     /locus_tag="VHI09_001047"
     CDS             complement(1992..3536)
                     /locus_tag="VHI09_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEQ6120931.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            complement(3784..4479)
                     /locus_tag="VHI09_001048"
     CDS             complement(3784..4479)
                     /locus_tag="VHI09_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEQ6120932.1"
                     /translation="MDFFAIDPIGLPHTSLIFYLSSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATINFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLALAFSKEK"
     gene            complement(4559..5950)
                     /locus_tag="VHI09_001049"
     CDS             complement(4559..5950)
                     /locus_tag="VHI09_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEQ6120933.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELSGKPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRNKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKE
                     LARLASQVYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVD
                     RLVTDKNCDSYQP"
     gene            complement(6148..7194)
                     /locus_tag="VHI09_001050"
     CDS             complement(6148..7194)
                     /locus_tag="VHI09_001050"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEQ6120934.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVIAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEIFDQRESHLI"
     gene            complement(7295..7891)
                     /gene="recR"
                     /locus_tag="VHI09_001051"
     CDS             complement(7295..7891)
                     /gene="recR"
                     /locus_tag="VHI09_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEQ6120935.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(8689..9468)
                     /locus_tag="VHI09_001052"
     CDS             complement(8689..9468)
                     /locus_tag="VHI09_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEQ6120936.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFVTNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            complement(9592..10134)
                     /locus_tag="VHI09_001053"
     CDS             complement(9592..10134)
                     /locus_tag="VHI09_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120937.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            complement(10295..10816)
                     /locus_tag="VHI09_001054"
     CDS             complement(10295..10816)
                     /locus_tag="VHI09_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEQ6120938.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILYKGI"
     gene            complement(10924..11619)
                     /locus_tag="VHI09_001055"
     CDS             complement(10924..11619)
                     /locus_tag="VHI09_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEQ6120939.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
BASE COUNT         3653 a         2431 c         2006 g         3654 t
ORIGIN      
        1 tccattataa aagcaacttt ttcagaaaat taaaaagagc tgactctcat caactctctt
       61 taagcttatt cacctttgtg acgaatcaca aaaccagtat ttttagcact tgattttttc
      121 tctttattga aaaagtcttt actattacgt tttgatttac gtttgaaatc gcggttgcca
      181 ctgccatcgt gaccatcacg cttatcacgt ttttgacgac gacggtcacc atcacgctca
      241 tctctgttac ggtcaccacg atagccgcca cggcgatcac cacggttacg gttgtcacgt
      301 ccacgaccac ctttgccatt tttattgccg tgaccaccac ccacatattt gaatggtaat
      361 ggtttttcac gtgcaatttc aacttccggt aagctatctg gatcttgaac cgttaagctc
      421 aaaatatata aagctaattc ttcaggagtg aattcggcag ccaattggac agcatcgcct
      481 ttgaatttat caaagtttga acgaatagtc tcatccgcaa agtcgcgttc aattttcttg
      541 agggctactt ttttcttggc ttgaaaggcc tcttcagcag ttgctggacg aagaggtttc
      601 atctgttttt tcgttaagtt ctcaatcatt gagaggtagc ccatctcatt tggtgagaca
      661 aaagtgatag actcaccaga tttaccggca cgacctgtac gaccaatacg gtgaacataa
      721 ctttctggat cttgggtaat atcgtagtta taaacgtggg taactcctga aatatcaagg
      781 ccacgcgctg ctacatcagt ggcaacaaga atatcaattt ggtcgttttt gaaatcacgg
      841 ataacacgta agcgtttatt ttggtcaaga tcaccatgga ttccttcggc acggaaacca
      901 cgaagtttca agccacgtgt aatttcatca actcggcgct tagtccgccc aaaaacgatg
      961 gataattctg gctggttaac atccatcaga cgggtcatag tatcaaattt ttcttgttct
     1021 ttcacacgaa catagtattg atcaacattg acgtttgtta attctttgtt cttaatttga
     1081 acatgttcag ggtctttcat gaatttaacc ccgatttgtt taataggtgc cggcatcgtt
     1141 gcggagaaga gcaaggtttg acggtctgct gggacacgac taataatagc ttcaatgtct
     1201 tccaagaatc ccatgttaag catttcatct gcttcatcaa ggattaaggt ttccacatga
     1261 tctaaaataa gagccttgcg cttaatcaaa tctaacaagc gacccggtgt tcccacgaca
     1321 atgtgggcac ctgatttaag ggctttgatt tgtttttcaa tacttgatcc accgtaaaca
     1381 gagcgaacct taaccccttt ttcacgtcca aaacggaaca attcttcctg actttgtaca
     1441 gccaattcac gcgttggtgc aatgaccaat gcttggatga tattttcgtt agtacgaatc
     1501 ttattcaagg ttggtagacc aaacgctgct gttttaccag ttcctgtttg tgcttgacca
     1561 ataacatcct tcccttctag tgcgagagga atcgtcattt cctggatagg agatgctttc
     1621 tcaaaaccag cggtaacaac agctgattgg atgtcttgtg ataagttaaa ttctgtaaat
     1681 ttcaattgtt tctcttttct aaaaaggcag tgcgaagctg ccttataaac cttttaattt
     1741 tcgtcgtttg attaacaacc taactagtgt aacacaatta gaatcaaaaa gataggaaat
     1801 aaggctaata taagcaataa cacgctttca cgattatccg ctaaatcata aatatgctta
     1861 cagttgtgaa gatatcgttt tttgatagaa tcttaaatct gcaaaagaga taactagcca
     1921 ctcatttctt ttaaaaagtt gctcagtaaa ttgatctcca aaacgttaga ctgttgaaaa
     1981 tcatgtttcg attacatttt ctcttctaaa gtgacatctg ggtatttgtc tgcaaaccag
     2041 cgaagggcaa aatcattttc aaataggaaa actggttggt caaaacgatc cttagctaaa
     2101 atgtttcggc ttgaagacat gcgttgatcc aaatcatctt ctgaaatcca gcgaacagtc
     2161 tttttaccca ttggtgtcat cacaacttca gcgttgtatt caccttccat acgatgttta
     2221 aagacttcaa actgcagttg acccacagca cccagcatgt attcgcctgt ctgataattc
     2281 ttataaagct gaattgctcc ttcttgtact aattgttcaa tacctttgtg gaaggatttc
     2341 tgcttcataa catttttcgg agaaaccttc atgaagattt ctggggtaaa agttggtaag
     2401 ggttcaaatt caaatttatt tttcccaact gttaaggtat ccccaacttg ataagttcct
     2461 gtatcataaa ccccgataat atccccagca acagcattcg tcacattttc ccgtgattct
     2521 gccataaatt gagtaacatt ggagagctta gcgcctttgc cagtgcgtgt caaattaact
     2581 cccataccgc gctcaaattc accagaaaca atacgcacaa aagcaatacg gtcacggtgt
     2641 ttagggtcca tgttagcttg aattttaaag acaaaacctg aaaaatcttt agctaagggg
     2701 tcaacaacat taccttctgt agtcttgtga ccatgaggtt ctggggcaaa ctccaagaaa
     2761 gtatcaagaa aagtctgaac tccaaagttc gtaagggcag agccaaaaaa gactggagtt
     2821 aggtcaccat caagaatagc ttgttctgaa aagtcattac ctgcttcttg aagaagttca
     2881 atatcttctt tgacttgttc ataaaaggga ttattggcaa acagctgatc accgtcttca
     2941 atacttgcaa aacgctcgtc tcccttatag agttctaaac gtttattatg taaatcatat
     3001 aagccctcaa aggcgcgtcc cataccaatc ggccagttca tcggataaga agcaatccct
     3061 aaaacttctt ctaattcttc caagagttct aaaggctcac gcccatcacg gtctaacttg
     3121 ttaataaagg tgaagacagg aatattacga tgtttgacaa cttcaaaaag ttttttcgtt
     3181 tgcgcctcaa tacctttagc cgaatctaca accatgacag ccgcatccac agccattaag
     3241 gtacggtatg tatcttccga gaaatcctca tgtccaggtg tatccaaaat attaacacgt
     3301 ttacccgcgt aatcaaattg cataacagat gaagtaacag agataccacg ttgcttttca
     3361 atatccatcc agtcggactt tgcaaacgta cctgattttt tcccttttac tgtccctgct
     3421 tctctaattt caccaccaaa atataatagt tgctcagtaa tagtcgtctt accagcatcc
     3481 gggtgagaaa tgatggcaaa agtacggcgt tttttaattt cttctgtaag tgacattctt
     3541 ttttccttgt atagtaaaat ggcagagctt ttctctgagt tattttcgtt aggtattact
     3601 tgtttatcaa aagagttggg ctacattggg tttctccata tacgattagc ctctaaagtt
     3661 cttcaaaact ggtattataa cttatcttgg gctatcaatg agtgtctttt ttaaaaaaat
     3721 cgagcctgga aatcatccca gactcttctt attatagctg attttaagtg ctttttcaac
     3781 gacctacttt tctttagaga aagcaagtgc cagctgttga gcttcaccaa atttaaggta
     3841 aaaatattcg gttattttaa ccataaaagt caagccactt gtaacgatca caaaattaag
     3901 tagcaaatga cgcgtatgaa tcactggaat atccatcaca aaaccataat ttcctttcgt
     3961 ggctaaactg accaacagaa gaagaaagtt aatagttgct aaatagcgta ctactgatag
     4021 cagtcttaac tgactggcat cgtaaaaacg caagaggtaa atcaaaccat tcactaacaa
     4081 agcataatga ccaagataaa acgagacatt tgctacatgc cataatctaa acggataaag
     4141 atctggcgat aaaagtgcaa ggaatgttcc acctatcccc aaaaccataa ataattgctt
     4201 aaacttgtta cgatcaggta gaaaaaagat agctaacata gcgatgcgac agtgatacaa
     4261 cggcaaagct tcatctaaag gaaaccccct taacacgtac catgtgtaca agccaatcac
     4321 ctgagataac tgtaaaaaca agaaaaaata acgataagat tttaacctat atgcttgaaa
     4381 agtcaaaaaa acaagcaaaa gcgctatcaa gagtgacgac aagtaaaaga taagtgaggt
     4441 atgaggcagt ccgatggggt caattgcaaa aaaatccata atcgtccctt tctatatttt
     4501 ttcattatac cttaatcttc atgagagaac aagcctttct ctgaaagttt tataaaaatc
     4561 aaggctgata gctgtcacaa ttcttgtcag tcactaatcg atctactaac ttttctagcg
     4621 acatcgagtg gctgcctttt aaaagaattt gatcaaaggg attaagaatg ttttgaacat
     4681 attgtgccat ggcttcaaac tggtcttctt gttcagtttt taaaaaataa tgtacttgct
     4741 cagcaggata aacctgactt gccaaccgtg ctaattcttt aatatgatcc ccataaaaaa
     4801 caagctgatc aatattacct gatgttaagc tgtcaattaa ctgagaatga agtatcactg
     4861 aatcctttcc aagttctttc atatctgcta gcactgcaat cttttttcct ccggggttct
     4921 ttgctatgtt agcaaatgtt tctaaaatca gacgcatggc tgttgggttg gcattgtaga
     4981 catctgataa aatatcagca ccattggctg ctttcttcca ctccgtacga ttacctgtca
     5041 acgtcacagt ctgtaaagca gctataatat cctcgtctgt tactgccaat agttttccta
     5101 cataggctgc taccatagca ttggtcgcgt tgtattttcc tggtagaggt agggacacag
     5161 gtgttgctaa cacatttgtt gtaaacgtta agctatcttt gtcttcttga ataccagtaa
     5221 cgtcaatttc ctgctgattt ccaaaacgga taaccatctg attttctgga agatatgggt
     5281 caataatcgg atctcccgga gcaattaaaa tcccatctga attcatgccg tcaacaatct
     5341 gcattttgcc ttgggctatt ttgtttcggc ttccaaaata ttctaaatga gcttcgccga
     5401 ctaaggttaa aactgcaata cgaggccttg caatttcaga cagaagacga atatctccca
     5461 tgtgatcttg tcccatttca agaacaattt tttccgtatc gtcaggcatg tggagaacgg
     5521 tgtaaggcaa accaatctca ttattgtaat ttccttgcgt tttgtaagtt ttgtaagtgg
     5581 ttgataggac agctcctatc atatctttgg tggaagtttt accatttgag cctgtcacag
     5641 caataacatc cacgcgcatc ttatcaatat aataatgggc tagcttttga aaagctttta
     5701 ggcagtcttc aacaagaagg tgaggtttcc ccgacaattc tttttctgaa aaagtagcga
     5761 cagctccatt ttgaaaggct aaatcaataa actcatgacc atccctttga ccttttaagg
     5821 gtaaaaagag gtctcctttt gtaatctttc ggctatcaaa ttcaatgtga tgcagaggaa
     5881 cgtcgtctaa gtcagacaca ttattttggg cgtcaactat tttagccact tcgtgtaagg
     5941 ttaatttcat tttgttttct ttctaagaac actagtattt acgatgtcct atctattata
     6001 gcacaaaaca ttagaaaaat ttctaaaatg tcaaactccc ttaatggctc tactattctt
     6061 cctaaaagag caacaaaaaa ggacgaacgt cacatcttga cgatgtttgc cgttccatcc
     6121 aagtcaatgt ttattcatct cataggttta aatcagatgg ctctcacgct gatcaaatat
     6181 ttcttgtgcc agtgtcacca gctcttcaat caaatctgga taagccaatc ccatattttc
     6241 ccataagagt ggatacatag accattgggt aaagcctggc atggtgttta attcgttaag
     6301 gtaaacctgt ccgtcttggg tcaagaaaaa gtcacaacgg gacaacccac aaccacctaa
     6361 ggctttaaag gctacttcag cataagaacg catttctgtc acaatactct ggtctacatc
     6421 tgctggaatg gccatcgtaa tcttattgtc aacatatttt gcttggtagt cgtaaaaatc
     6481 aacgtccttg attacttctc ctggtagagt tgatttaacc ttatcatttc caaggagacc
     6541 aacttcaatt tctctggcaa tcacgccttg ttcaatcaag acacgactgt cataggtcag
     6601 ggctaactga atcgcttttc ttaattctac ttttgtttgg gctttggaaa tccccacaga
     6661 agaacccata ttagctggtt ttacgaagat aggaaaagta agtcttgcta aagtttcaac
     6721 taaacaagcc tccaaatctt gtccgtcaat ataaacagta taggcaactt gtggaattcc
     6781 aattgattct aagacacgtt tagtagtaat tttatccata gctatgctag aagacatgac
     6841 attagtccct atatatggca ttctaagcac ttctaagaaa ccttggatag agccatcttc
     6901 tcccataggt ccatgtaata caggaaatac aactgcacct tcttcataga tatcacttgg
     6961 ttttattttt tgagttagct ctatcgtctc atttgtcatc aagcgttctg attccgatgg
     7021 cttttctgaa aattgctgag ttttgataaa ttggcccatc tgagtaataa aataagtttt
     7081 tactaagaac ttatcatagt tgactgctcg catcacactc tctgctgaaa gcactgagac
     7141 ttcacgttct gctgagcggc ctccgtataa tagaactaac gtttgtttgg acatagctaa
     7201 cctttcttat ctgaattgaa tgttttttta ttataacaaa aagatgtagc cttttcaaaa
     7261 cctctctttg cgagtaaagg caccaacatg atcattaaag ttcagtacga ttctcaatag
     7321 ctctcaataa tgttacttca tcagcatact caatatctga acctactgcg agacctcttg
     7381 ccaaacgagt taccttaatc ccggctggtt tcaagacacg tgaaatatac atggacgttg
     7441 cttccccatc tgctgtggca ttggtagcta cgataacttc gctcacctta ccatccatta
     7501 gacgggtaat taaactttta aggttgatgt catctggccc cacaccattc ataggcgaaa
     7561 tcaagccgtg aagcacatga taatagccat gatactcttg gattttttcc atggcagaaa
     7621 catctttagc atcttctact accagaatgg tcgtctgatc acgactcgtg tctgtgcaaa
     7681 tgtgacaagg atcgtcatcg gtaaggtttc cacaaatcga acaataggtc agttctcttt
     7741 tagctgctaa taagttttta gcaaaatcat tgacatcttc atttgacatt ccaatagtat
     7801 aaaaggctaa tctcgtcgcc gtcttgatac caattcctgg aagtttagag taactgtcaa
     7861 ttaactttgc aatgggtgtt ggataaagca ctaagttctc ctttattgtt ttccatatat
     7921 cgccacataa agcgccctca catctttgcc gatatattcg taatatgtgg atacataaca
     7981 gcaaccgcaa tattatcttc gttatttgta ttataagcta ctacatttaa gttgtaagtt
     8041 actaaggtct gatctgaatt gtctgtgata caagtctcag cagttccaat tttagcgtga
     8101 atcgtgacag aaccgtttgg attagccaag taaaaacttt tttactcgac ttgttacctt
     8161 ggccttgaga tatttggctt ttgttttttg attcatgtta cttttttaga aaccgcaaac
     8221 catccttatg atataagttt ataggaattg tttcattcca tgcagaaatg atgtttactc
     8281 aattgactat gctatttttc tcaacaatag tgcataaagc aaactcatgg ttatctttaa
     8341 gctttcatgc tacactttct tcactaaaag tatattacca aaagcagtat taagaaatct
     8401 ctaagcacaa accataagac agtttgtacg cctgatgaat ctgttgctgc atcactgtcc
     8461 actttttacc ctaacaaggc tatttttgaa agacaactaa tgatctacat tattttggga
     8521 actactccca tagcaaattc tttttacgga ttttttggga atactcattt tttagggaat
     8581 aaactaacca ttatttagct aaatttttta aacttcgctt cttattttac cataaattat
     8641 aaaaagaaga ctgagaatag gacgccctca gtctaaaaaa atattttctc attgagtttt
     8701 actcttttgc ggatgatagt aaactttaaa atataaaaaa ctaacaaaaa tggctccacc
     8761 gataatatta ccactataga caaaaataaa attagtgaca aaatccaacc atgttgctcc
     8821 tccttcaaaa atagcagcag gaatgacaaa agcatttgct acactatgct gaaaaccaag
     8881 ggctacaaag gtcatgacag gaaaccaagt accaaggaat ttaccagcag catcatttgc
     8941 cccatagcat agccacaagg ccaaaccaac aaaccagtta caaccaattc ctgatactag
     9001 tgcctgcaaa gggctcgctg ctattttagc atgggccacc tcaatcactt cttctttaaa
     9061 aataccagct gatgtcaagc ctagaaaatg accaaacaca aaagctacaa aaacagctcc
     9121 aatcacatta aacaaggtaa tgataagcca attcttggca agctcgctaa acttaatttt
     9181 tttggccaac aaagctgcag aaacagccat catgttccct gtaattaatt ctccacccgc
     9241 cattaaaata ataattagac caatcggaaa agcacaagct cctacgatac tggaaaaagc
     9301 accaaaagtt tctaaaccac tggcggcaat tctcacgtag agtaaataac caaggctaat
     9361 catcgctcca ccaataaagc ctaagataga tttagccaaa aaagttttgg taaccttatg
     9421 ttccccaata tgaatagttg cttctaaaat ctgttctggt gttttcatga tatctccttt
     9481 tgttactgta ttcacaaaca cacactttag tatatcaatt ttcctaaaaa atgcaagccc
     9541 tttcaacaag aaagggctcg tcattatcat ataactatct ctattttagt cttaggcatt
     9601 tatggtggga agttttaagt tttttgctaa aaaacgccat agaactaaag caagatagaa
     9661 atccaatatg ccatgaataa gcgaacctaa gccaatcaag cctagcatag accaaaaata
     9721 agacatagaa gttgctgtgg ttgctgttaa aatatagaca actaaaaatt cagccaagcc
     9781 atgaatgaca ttaatgaaaa acgcaaaact aaaacatttt actgccgatg tcatcagttg
     9841 tggttttcta cttaaccacc acgctgccaa aatagcaaac aataaatgag agagtgctct
     9901 taaaacaata attaaaggaa gtccagctaa taaaaaacct aatgtggtac caagagcgac
     9961 caaaattgct actggaatcg acataaaaat agccaaaaac aaaggaacat ggctcgccag
    10021 agtaaaggaa gctggcccaa taataagctt aagaggcatg attatcggaa tcaaaatcgc
    10081 aaatgcgacc aaaatagagg tataagctat taattgtgtt ggttttttag tcattttatc
    10141 tccttttcaa aaatagtata ccttaaaaac caataactat caagacacct gtatcttttg
    10201 gtatttttaa acggtacctt gttgctgata aataatcaat agaatatttt agcatggtat
    10261 tcataacaac catcaatggc ataatatact gtagttatat tcctttgtat aagatacctg
    10321 ctaattctaa atcatgcttg atagctaaaa aggcttcttg actaggacac gaaattaagt
    10381 gactatgtag tccttctgtc aacgaagaca ataactctgc tttagattga ctgagtttgg
    10441 acatgaaatt agttacatca gagtgtgtct taatatttaa aggggctgtt atcataccgt
    10501 aaataggatg ttctacctct accgtcgtaa taatcccttg atgagctaaa ataatttcta
    10561 attctttttt agtttcttct acattatgtt ggcacacaat acgtgcacaa aattggtgac
    10621 tataaagcgc tgttgacata atataacctt ttggagtcga aataatatca atctgctgag
    10681 ctcgaagaag agctatatct ccaacaatta cttggcgact cactcctaat aacttaccta
    10741 aacgagtagc agatacagct ttttgttcac tatttaagca ctcaataatt ttttgacgac
    10801 gatcttctgc tttcataatc tatctatcct tttttatttc tatcatatca tatttttaag
    10861 cagggttttc tttcttctta ggatatagaa cccaaaaaac cagaccaaag tctggttttt
    10921 aagttattca ccaccaaggt agtattcttt aacaatgtta agtttttcat ctaattcaaa
    10981 aacaagtggt gggaagtttg gaatttccac atccatgatt tcgtcatctg aaagaccttt
    11041 aatgtgtttc acaagagcgc ggattgagtt accatgtgca ccaacaaaga cgtttttacc
    11101 gtcaagaaga gctggagcaa ttttttcttc ccagtaaggc attgcgcgtt caagagttac
    11161 ttttaagttt tcagcatctg gaataagggc tgggtcaaga tcagcataac gacggtcttt
    11221 gtgtgctgaa tactcatcat ctttagccat agctggcggc aacacgtcgt atgaacgacg
    11281 ccagatatga acttgttcat caccaaattg ttctgcagct tcagctttgt ttttgccagt
    11341 caaagcacca tagtgacgct cgttcaagcg ccatgatttt tcagttggaa cccacaattg
    11401 acctgcattt tcaagggcaa ggttagttgt tttgatagca cgtgtcaata ctgaagtgaa
    11461 agcaaggtca aattcaatac ctgcttcttt gatcaattta cctgcatcaa tcgcttgttg
    11521 tgtccctttt tctgaaagat caacatctgc ccaaccagtg aagaggttag ctttgttcca
    11581 ttctgactca ccgtggcgag cgaaaaccaa ttttaccatg agttaatatc tccttttaat
    11641 ttaaaggttg ctaccttttt acaattccta ttttacaaaa aatatagcaa aaaagctagt
    11701 ctattctgac tttattgaaa atcataatgt gactatcatg aaaa
//
LOCUS       DAUELZ010000050        11528 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.051, whole genome shotgun sequence.
ACCESSION   DAUELZ010000050 DAUELZ010000000
VERSION     DAUELZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.051"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            63..1877
                     /gene="dnaG"
                     /locus_tag="VHI09_001056"
     CDS             63..1877
                     /gene="dnaG"
                     /locus_tag="VHI09_001056"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ6120940.1"
                     /translation="MGLLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     ELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            1886..2995
                     /gene="rpoD"
                     /locus_tag="VHI09_001057"
     CDS             1886..2995
                     /gene="rpoD"
                     /locus_tag="VHI09_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEQ6120941.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            3231..3569
                     /locus_tag="VHI09_001058"
     CDS             3231..3569
                     /locus_tag="VHI09_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEQ6120942.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            3707..4561
                     /gene="rfbD"
                     /locus_tag="VHI09_001059"
     CDS             3707..4561
                     /gene="rfbD"
                     /locus_tag="VHI09_001059"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEQ6120943.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDCPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            4680..5834
                     /locus_tag="VHI09_001060"
     CDS             4680..5834
                     /locus_tag="VHI09_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEQ6120944.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQNQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSRPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTYFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEGNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWSK
                     QKGQLAELINQVDAGFDSDHLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            5824..6756
                     /locus_tag="VHI09_001061"
     CDS             5824..6756
                     /locus_tag="VHI09_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ6120945.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLNPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            6759..7562
                     /locus_tag="VHI09_001062"
     CDS             6759..7562
                     /locus_tag="VHI09_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6120946.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNIPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYIVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            7562..8767
                     /locus_tag="VHI09_001063"
     CDS             7562..8767
                     /locus_tag="VHI09_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6120947.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            8792..9799
                     /locus_tag="VHI09_001064"
     CDS             8792..9799
                     /locus_tag="VHI09_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ6120948.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTVYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEVSKLLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            9796..>11528
                     /locus_tag="VHI09_001065"
     CDS             9796..>11528
                     /locus_tag="VHI09_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEQ6120949.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMAPYFENFEEKETVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKKTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSVFETDDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLFVYI
                     AWGNSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPA
                     KAMKYIFLRLM"
BASE COUNT         3809 a         1895 c         2283 g         3541 t
ORIGIN      
        1 tcacttcagt ttttaaattt acaaaaaaga gtttcggtgg tataataaaa cgtaaaacta
       61 aaatgggatt gttatgggga ggtgacgatt tggcaattga caaagaaatg atttcccaag
      121 taaaaaatag cgttaatatt gtcgatgtca ttggagaagt ggtcaaactt tcccgatcag
      181 ggcggcatta tctcgggctt tgcccatttc ataaggaaaa gacaccctct tttaatgttg
      241 ttgaagacag acaatttttt cactgctttg gctgtggaaa atcaggggat gtttttaaat
      301 ttattgagga ataccgccaa gtccccttct tagaaagtgt tcagattatt gccgataaga
      361 ctggtatgtc gcttaatatc ccgccaagtc aggcagtact tgctagccaa cacaagcacc
      421 ctaatcacgc tttgatgaca cttcatgagg atgctgctaa attttaccat gcagttttga
      481 tgaccactac cattggtcaa gaagctagga agtaccttta ccagagaggt ttggatgacc
      541 aattaattga gcatttcaat attggtttag ccccagatga gtcagattat ctttatcaag
      601 ctctttctaa aaaatacgag gaaggtcaat tggttgcttc aggattgttt aacttgtccg
      661 atcaatctaa taccatttac gacgcctttc gaaatcgtat catgtttccc ttatcagatg
      721 accgagggca tattattgcc ttttcaggac gtatctggac ggcagctgat acagaaaaga
      781 gacaggccaa gtataaaaat tcaagaggaa cagttctttt taacaaatct tatgaattgt
      841 atcatctgga caaggcaagg cctgttattg ccaaaaccca tgaagtattt ctaatggaag
      901 ggtttatgga cgtgattgcc gcttaccgtt ccggctatga aaatgctgtt gcttcaatgg
      961 ggacggcatt gactcaagaa catgtcaatc accttaagca agtcactaaa aaagttgttt
     1021 tgatttatga tggtgacgat gctggacaac atgctattgc aaaatcacta gaattgctta
     1081 aagattttgt tgtcgaaatt gtcagaatcc ccaataaaat ggatcctgac gaatttgtac
     1141 aacggcattc cccagaagca tttgcagatt tgcttaagca gtcacggatc agtagtgttg
     1201 aattttttat tgattaccta aaacctacta atgtagacaa tttgcaatca caaattgttt
     1261 atgtggagaa aatggcacca ttgattgctc aatcaccatc catcacagct caacattcgt
     1321 atattaacaa gattgctgat ttgttgccaa actttgacta ttttcaagta gaacaatcag
     1381 taaatgcatt aaggattcaa gataggcaaa agcgtcaagg tcaaatagct caagctgtca
     1441 gcaatcttgt gaccttacca atgccaaaaa gtttgacagc tattgctaag acagaaagtc
     1501 atctcatgca tcggctctta catcatgact atttattaaa tgaatttcga catcgtgatg
     1561 atttttattt tgatacctct accttagaat tactttatca acggctgaag caacaaggac
     1621 acattacatc ttatgatttg tcagagatgt cggaggaagt taaccgtgct tattataatg
     1681 ttttagaaga aaaccttccc aaagaagtag ctcttggtga gattgatgat attttatcca
     1741 aacgtgccaa acttttagca gagcgcgatc ttcacaaaca agggaaaaaa gttagagaat
     1801 ctagtaataa aggcgatcat caagcggctc tagaagtact agaacatttt attgcgcaga
     1861 aacgaaaaat ggaatagagg aaattatgac aaaacaaaaa gaaataacaa cttttaacgt
     1921 tcaagttgcg gagtttattc gccatcacaa aaaagaagga acagcgattg atgatgacgt
     1981 gaccgaaaaa cttgtcattc catttgcttt ggatgctgat cagattgatg acttacttga
     2041 gcggttaaca gatggtggta tctcgattac ggataaagaa ggtaacccat catcaaaata
     2101 cattgttgaa gaaccaaaac cagaagaatt aactgatgaa gaattaattg gtagtaattc
     2161 agctaaagta aacgaccctg tccgcatgta cctcaaagaa attggtgtgg taccgctttt
     2221 aacaagtgaa gaggaaaaag aattggctgt tgcggttgcc aagggagatt taatggctaa
     2281 acaacgttta gcggaagcaa accttcgtct tgttgtctca attgcaaaac gttatgttgg
     2341 acgtggaatg caatttcttg atttaattca agaaggtaac atgggactca tgaaagctgt
     2401 tgataaattt gattattcaa aaggattcaa attctcaact tatgcaacat ggtggattcg
     2461 tcaagctata acacgcgcaa ttgcagatca agcacgtacg attcgtatcc ctgttcatat
     2521 ggttgaaacg attaataaat tagtccgtga acaacgtaat cttcttcaag aacttggtca
     2581 agatccaacg ccagaacaaa tcgcagaacg tatggaaatg actccagaca aggttcgtga
     2641 aattctaaaa attgctcaag aacctgtttc tttagaaaca ccaattgggg aagaagatga
     2701 tagtcactta ggtgatttta tcgaagatga agttattgaa aatccagtag attatacgac
     2761 gcgtgttgtt cttcgtgagc aattggatga agttttagat acgcttactg accgtgaaga
     2821 gaacgttctt cgcctacgct ttggccttga cgatggtaaa atgcgtacgt tagaggacgt
     2881 aggtaaagtc tttaatgtta ctcgtgagcg cattcgacag attgaagcta aagcccttag
     2941 aaaacttcgc catcctagtc gtagtaaaca attaagagac tttatagagg actaaaatcg
     3001 tccagtggac gattttaggt cgttcctttg aaataagaaa ggagcgaaaa tcccaagtgg
     3061 cacttatcag cggtacacct acaaattaga aaacggaccg aatgtggtgg gtatttttaa
     3121 gggtagactt gatatcttaa acaagcccag agggcaattc ctctaaatag gcaaagcttt
     3181 agtggttttt ttacttatct acgatatatt caaaaaatga aaggaaagcc atgtctgata
     3241 caccaaaata tacacaagat caggtgatag ctatcaagaa tagaattctt gaagctttag
     3301 aaactgttat tgatcccgag ctaggtattg atgttgttaa tctaggcttg atttatgaaa
     3361 ttcggtttaa tgataacggt tatacagaga ttgatatgac actcacaaca atgggttgtc
     3421 ctttggcaga cttgttgaca gattacatcc atgatgccct gcaagatgtg ccagaagtca
     3481 ctaagactga ggtcaaatta gtctggtacc ctgcttggac ggttgataaa atgagccgct
     3541 atgctaggat tgcgctgggt attcgctaat cattgatagt aaagggatga aatattccct
     3601 tttttgatgt agaataaaag gaaatacagg tgaaagcctt tggttaatgt tagagaacac
     3661 taagatactg agaaaagtgt tctacctatg gcaaaaagga gaactgatga ttttaattac
     3721 aggaagcaat ggtcaattag ggacagaact tcgatactta ttagatgaac gaggtgttga
     3781 ttatgtagcc gttgatgtcg cagaaatgga catcacaaat gaagacaaag tcgaagctgt
     3841 ttttgcgcag gtcaagccaa cattggtcta tcattgtgct gcttatacgg cagttgatgc
     3901 agcagaagac gaaggaaaag ctttgaatga ggctattaat gtaacaggct cagaaaatat
     3961 tgccaaggct tgtggaaaat atggggctac ccttgtttac atttcaacag actacgtatt
     4021 tgatggcaat aaaccagtcg gtcaagaatg ggtagaaaca gattgtccag accctaagac
     4081 agaatatggc cgtacaaaac gtttaggaga actagctgtt gaacgatacg cagagcattt
     4141 ttatattatc cgtactgctt gggtatttgg aaattatggt aaaaactttg tctttaccat
     4201 ggagcagtta gcggaaaatc actcccgttt gactgttgtc aatgaccaac acggtcggcc
     4261 aacgtggaca agaaccttgg cagaattcat gtgttatttg acagagaatc aaaaagcctt
     4321 tggttactat cacttgtcaa atgatgctaa ggaagacacc acttggtatg attttgccaa
     4381 agaaatctta aaagacaagg ctgttgaagt ggtgccagtt gattcatcag ctttcccagc
     4441 aaaagccaaa cgtcctttaa attcaaccat gaatcttgat aaggcaaaag caacagggtt
     4501 tgttattcca acatggcaag aagccttgaa ggcattttac caacaaggac tgaaaaagta
     4561 agtattctaa aaagagttgg ctttctaagg ccaactcttt ttcatgggtt taaggtcgca
     4621 ttagccttct aattgtggta taatatcact gattatattt tagaatgggg tagtatgcaa
     4681 tgcaggatgt ttttatcatt ggtagcagag ggttaccagc gaaatacggt ggctttgaaa
     4741 cctttgtcga agagttgatt agtcatcaat catctaaaaa cattcgttat cacgttgctt
     4801 gtttaagcga tacgaagcat aaggttcatt ttgattataa gggggcagat tgcttttacc
     4861 ttaatccccc aaaacttggt cctgctagag ttattgctta tgatatgatg gcaattacct
     4921 atgccctgtc ttatagcgac caaaaccaga ttcagaaccc tattttttat gtgttgggca
     4981 atactgtagg cgcttttatt gcgccatttg tgaagcagat tcacaatcga ggtggacgct
     5041 tttttatcaa tccagacggg ttagagtgga aacggtctaa atggtcaaga ccagtgcagg
     5101 cctacctgaa gttttccgaa aagcaaatga ctagacaggc tgatttggtc atttcagata
     5161 atattggcat tgaccgctac cttaaacagg tttatccttg gtctaaaaca tattttattg
     5221 cttatggaac gcagacacaa ccttcacgtt tgacaacagc agatagcaag gtcagagctt
     5281 attttcaaac ctttgatatt agagaaaagg attactatct cattttagga cgctttgttc
     5341 ctgaaaataa ttacgaaact gctattaaag aatttatggc ttcctcaact aagcgtgatt
     5401 tagtgattat ttgtaatcat gaaggcaatg cttactttaa gcaattactt gctgagacag
     5461 agtgtgataa agacccacgc attaaatttg ttggtaccct ttatgataaa gaactattag
     5521 cctacattcg agaacaggct tatgcctata tccatggtca tgaggtagga gggactaatc
     5581 caggccttct agaagcacta gcccatacca atcttaattt ggttttagga gttgatttta
     5641 accagtcggt ggctaagtct gctgcgctgt attggtccaa gcaaaaaggg caattagcag
     5701 agctgattaa ccaagttgat gcaggttttg atagtgatca tctgggcaaa gaagccaaag
     5761 ctattattca agaacattac acttgggaaa aaattgtagg ggagtacgag gctttatttt
     5821 taaatgaaca ttaatatttt actatccacc tacaatggag aacgcttctt agcagaacaa
     5881 attcaaagta tccaaaggca aacagttaac gactggacct tattgattag agatgatgga
     5941 tcaactgatg gcacccagga tattattcgc acatttgtca aggaagacaa acgaattcag
     6001 tggatcaatg aggggcagac agaaaatctg ggagttatta agaattttta caccctctta
     6061 aagcaccaaa aagcagatgt ctattttttc agtgaccaag atgatatatg gttagacaat
     6121 aaactggaag tgaccttatt ggaggctcaa aaacatgaaa tgacagctcc tttattggtt
     6181 tatacagacc tcaaagtggt aacccaacat ttagctattt gccatgacag tatgattaag
     6241 acccaatctg gtcatgcaaa cacaagccta cttcaagaat taaccgaaaa taccgtaaca
     6301 ggcggcacaa tgatgattac tcatgctctg gctgaggaat ggaccacttg tgacggttta
     6361 ttgatgcatg actggtattt agctttacta gcttctgcta ctgggaaatt agtttacctt
     6421 gatattccaa cagaattata ccgtcagcac gatgctaacg tgttgggagc tagaacttgg
     6481 tctaaacgca tgaaaaattg gttaaacccc catcatttag tcaacaaata ttggtggttg
     6541 attacttcaa gccaaaaaca ggctcaatta ctattagatt taccattaaa accaaatgac
     6601 catgaattgg taacggccta tgtttcttta ttggatatgc cttttaccaa acgcttagct
     6661 accttgaaaa ggtacggttt tagaaaaaat cgcatctttc acacctttat ttttcgtagt
     6721 ttagttgtta ccttatttgg ttacaggaga aagtaagaat gaatttttta acaaagaaaa
     6781 accgtatttt actgagagaa atggtaaaaa cggattttaa attacgctat caagggagcg
     6841 caattggcta tctatggtct atcttaaaac cgcttatgat gtttacgatt atgtacttgg
     6901 tgtttattcg ttttcttcgc ctgggtggaa atatacctca ttttccagta gcgcttttat
     6961 tggcaaatgt catctggtct tttttttcag aagcaactag catgggaatg gtatctattg
     7021 tatctcgggg agacttgttg cgaaaattga acttttctaa gcacatcatt gttttttcgg
     7081 cagtgttagg agctttaatt aattttctta ttaatttggt tgttgtttta atttttgcgt
     7141 tgataaatgg tgtgactata tcagggtatg cttatctctc tctttttctt tttatagaat
     7201 tagttgtttt agtgcttgga attgcattgt tattgtcgaa tgtctttgtt tattatcgtg
     7261 acttagctca agtctgggaa gtactattac aagcaggtat gtatgccact ccaatcattt
     7321 atccgatcac ttttgtttta gatagccacc ctttggcggc aaagttgttg atgctaaatc
     7381 cagtagcaca aatgattcaa gattttcgtt atttattgat tgacagggcc aacgtaacga
     7441 tttggcagat gtcaaccaat tggttttaca ttgttattcc atatttagta ccatttgtta
     7501 tattatttat tggcatcttt gtctttaaga aaaatgccga tagatttgcg gagattattt
     7561 aatgattaat agagaaattg cagtaaaagt acagcatgtt agtaaaacat ttaaactgcc
     7621 tacagaagca actaaaagtt ttcgcacgac tctggttaat cgcctaagag ggattaaagg
     7681 atttactgaa caacatgtgc taaaagatat taactttgat gtttataaag gcgatttttt
     7741 tggaattgtt gggcgtaacg gctcaggaaa atcaacgctc cttaaaatta tttctcagat
     7801 ctatgttcca gaaaaagggc aggttacagt agatgggaag atggtttctt ttatagagct
     7861 tggagtggga ttcaatccag aattaacagg acgagaaaat gtctatatga acggcgccat
     7921 gctgggattt accaaagacg aagttgatga tatgtacaat gatattgttg attttgcaga
     7981 gttgcatcat tttatgaatc aaaaattaaa aaattattct tctggcatgc aggtacgttt
     8041 agcattttca gtggctatta aagcccaagg tgacgttctt attttagatg aggtgcttgc
     8101 tgtaggagat gaagcttttc agcgaaaatg taatgattat tttatggaaa gaaaagacag
     8161 tggtaagacg actattttag tgacacatga tatgggagct gttaagaagt attgtaaccg
     8221 tgcagttctt attgaagatg gtcttgttaa agcatatgga gaaccatttg atgttgctaa
     8281 tcagtatagt gtagataata cagagacagc tgaggatgca atgaatgctg aaaaaatatc
     8341 tgtatctgat attgccaaag atttgaaagt gtcattaatc tcaaatccaa gaataacacc
     8401 aaatgacaca atcacatttg aagtttctta tgaagtttta aaagatgatt ttacctacat
     8461 tgccttttct ttaacggata ttgatagaaa catttgggtt tacaacgaca attctttaga
     8521 ttatccaaca caaggtatag gtagaaaatg catttcttac caatgtcaac tatctcaact
     8581 taatgatata aaactaaaat tagaagtaac tgtccgagat aagaacggtc agatgctact
     8641 cttttcaaca gcagaacaat caccccagat agtgattcaa agaaacgata ttgatcaaga
     8701 tgatctgtca gctctagatt cagcaagtgg cttgtatcaa cgtaatggga agtgggtttt
     8761 tcattaatga gtaatagcta gaagggaatt tatgtataaa gtttcaatta tttgcaccaa
     8821 ttataacaaa gctccttgga tatctgatgc acttgacagt tttttaagcc aagtgacaga
     8881 ttttgaggtt gaaattattg ttattgatga tgcttctaca gatgactcac gagagatttt
     8941 aaagagttat caaaaaaagt cttcaggaaa aattaaatta ctctttaacg aaactaatat
     9001 tggaataaca aagacttgga taaaagcatg cctttatgca aaaggcaaat atattgctag
     9061 atgtgatggt gatgattact ggacggatag ttttaaactc caaaaacaag ttgatgtctt
     9121 agaagcttcc aaacggtcac gatggtgtaa tactgatttt gactttgtaa atagtcaagg
     9181 cgacttatta tatgctaatg ctttttcatc tggtcataca cctttgacag atacttacga
     9241 aaaagtttta gccttaaaag ggatgacgat ggcatcgaca tggctagttg atgctgattt
     9301 aatgagacaa gtaaaccaaa aaatcaatgt agatacacca gacgatactt ttgatattca
     9361 actagagtta tttcaactga ctcaactgac ctatattaaa gactccacga cggtttatcg
     9421 catgacaacc gattctgatt caagacctac tgatacacca aaaatgattc atcgtattca
     9481 aaaactactt gatactcagt taaattactt aaaaaagtat aatcaggtag atacaaaaga
     9541 agtgtctaaa ctattattac aacaagatgc taagcaagaa atacgaattc atgagttgag
     9601 ttgctttatt caagaattac aacaaactat cgtagataaa acaaagcagc aagaaacacg
     9661 tgaagtggag ttgcaaaatg tcatagaaga acaaaaaaat caactatctg aattaagaca
     9721 acaataccat gccattatta attcacgtca atggaaatac acgtctaagc tcattgcttt
     9781 tattaggaga aaaaaatgaa tcgtatttta ttatatgttc acttcaacaa atacaataaa
     9841 ataagtgcac atgtttacta tcaattggaa caaatgagat cactgttttc taagattgtt
     9901 tttatctcaa atagtaaagt gagtcacgaa gatttaaaac gtctcaaaaa ccactgtctt
     9961 attgatgagt ttttgcaacg caaaaataaa ggttttgatt tttctgcatg gcacgatggc
    10021 ttgattacta tgggatttga caagttggaa gagtttgact cattgacgat tatgaacgat
    10081 acttgttttg gtcctatttg ggagatggct ccttattttg aaaattttga agaaaaagag
    10141 acagttgact tttggggaat tactaataat agaggtacaa aagcttttaa agaacatgtc
    10201 caaagttatt ttatgacgtt taaaaatcaa gtcattcaaa acaaagtttt tcaacaattt
    10261 tggcaatcga taatagaata tgaaaacgtt caagaagtga ttcagcatta tgaaacgcaa
    10321 ttaacatcca ttctcttaaa tgaaggcttt agttatcaga cggtctttga tactagaaaa
    10381 gctgagtcgt cattcatgcc acatccggat ttttcatatt ataatccaac agcaattcta
    10441 aaacaccatg tgccttttat aaaagtaaaa gctattgatg ctaaccagca tatcgctcct
    10501 tacttactga atctgatacg agaaacaacc aattatccta tagatcttat tgtttcacat
    10561 atgtcgcaaa tctctttgcc agatgctaag taccttttat ctcaaaaata cctaaactgc
    10621 caacggttag caaaaaaaac ttgtcaaaag gtagctgttc atttacatgt tttttacgtt
    10681 gatttgctag atgaattttt aacagctttc gaagactgga acttccatta tgatcttttt
    10741 attacaacag atagtgatat caaaagaaaa gagatcaaag aaatacttca aagaaaaggt
    10801 aaaactgctg atatacgcgt tacgggaaat agaggaagag atatttatcc aatgttatta
    10861 ctaaaagata aactttctca atatgattat attggtcatt tccatacgaa aaaatcaaaa
    10921 gaagctgatt tttgggcagg agaatcttgg cgaaaagaat tgattgatat gctcgtgaaa
    10981 ccagcagata gtattcttag cgtttttgag acagatgata ttggtattat aatcgcagat
    11041 attccaagtt tttttagatt taataagatt gtcaatgctt ggaatgagca tttaatcgct
    11101 caagaaatga tgtctttgtg gagaaaaatg gatgttaaaa aacaaattga ttttcaagct
    11161 atggatacat ttgttatgag ttatggaaca tttgtatggt ttaaatacga tgctctaaaa
    11221 tcactttttg atttagaatt aactcaaaat gatattccaa gtgaaccttt accacaaaac
    11281 tctatattac atgcgattga gcggttattt gtctatatag cgtgggggaa ttcttatgat
    11341 tttaggattg ttaaaaatcc ctatgagtta acacctttta ttgacaataa actgttaaat
    11401 ttacgtgaag atgaaggagc tcatacctac gttaacttta atcaaatggg cggtatcaaa
    11461 ggagcattaa aatatattat cgttggtccc gctaaagcaa tgaagtatat attcttgcgt
    11521 ttaatgga
//
LOCUS       DAUELZ010000051        11356 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.052, whole genome shotgun sequence.
ACCESSION   DAUELZ010000051 DAUELZ010000000
VERSION     DAUELZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.052"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            90..1373
                     /locus_tag="VHI09_001066"
     CDS             90..1373
                     /locus_tag="VHI09_001066"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEQ6120950.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
     gene            1590..2165
                     /locus_tag="VHI09_001067"
     CDS             1590..2165
                     /locus_tag="VHI09_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEQ6120951.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
     gene            2422..4026
                     /locus_tag="VHI09_001068"
     CDS             2422..4026
                     /locus_tag="VHI09_001068"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEQ6120952.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRSEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            4194..5120
                     /locus_tag="VHI09_001069"
     CDS             4194..5120
                     /locus_tag="VHI09_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ6120953.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIKENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            5174..5259
                     /locus_tag="VHI09_001070"
     tRNA            5174..5259
                     /locus_tag="VHI09_001070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5208..5210,aa:Leu,seq:aag)
     gene            5465..6346
                     /locus_tag="VHI09_001071"
     CDS             5465..6346
                     /locus_tag="VHI09_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEQ6120954.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            6728..6916
                     /locus_tag="VHI09_001072"
     CDS             6728..6916
                     /locus_tag="VHI09_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEQ6120955.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            7068..7433
                     /locus_tag="VHI09_001073"
     CDS             7068..7433
                     /locus_tag="VHI09_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ6120956.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            7433..9097
                     /locus_tag="VHI09_001074"
     CDS             7433..9097
                     /locus_tag="VHI09_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEQ6120957.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKNKA
                     EVKDFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSNPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            9334..10224
                     /locus_tag="VHI09_001075"
     CDS             9334..10224
                     /locus_tag="VHI09_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEQ6120958.1"
                     /translation="MLEPFIFIAFGVIIILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHIRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFAAKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
     gene            10454..11308
                     /locus_tag="VHI09_001076"
     CDS             10454..11308
                     /locus_tag="VHI09_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEQ6120959.1"
                     /translation="MKSKKVVSVISLTLSLFLVTGCAKVDNNKSVNRKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
BASE COUNT         3765 a         1865 c         2450 g         3276 t
ORIGIN      
        1 gtttgatttc acgcataaaa agctttaaaa acgctttaaa ttatgataaa atgaactgta
       61 tcgaaattat tttaaggaga aatgacttaa tgtctacatc atttgaaaac aaagctacaa
      121 atcgtggcgt gattacattt acaatcagtc aagataaaat aaaaccagct cttgataaag
      181 cttttaataa aatcaaaaaa gacttaaatg caccaggttt ccgtaaagga cacatgcctc
      241 gtccagtctt caaccaaaaa tttggtgaag aagttcttta tgaagatgct ttgaatattg
      301 tattgccaga agcttatgaa gcagctgtga cggaacttgg tcttgatgtg gttgcacaac
      361 caaaaatcga tgttgtgtca atggaaaaag ggaaagagtg gacactttct gctgaagttg
      421 tgacaaaacc tgaagtgaaa cttggtgatt ataaaaacct agttgtagaa gttgatgctt
      481 caaaagaagt ttcagatgaa gacgtggatg ctaaaattga gcgcgaacgt caaaaccttg
      541 cggaactcat tattaaagac ggtgaagcag ctcaaggtga cactgttgtg attgactttg
      601 ttggttcagt tgatggtgtt gagtttgatg gcggtaaagg agataacttc tctcttgaac
      661 ttggttcagg acaatttatc ccaggttttg aagatcaact agttggtgct aaagctggcg
      721 atgaagtaga agttaatgtc acattcccag aatcttacca agcagaagat cttgcaggta
      781 aagccgctaa atttatgaca actattcacg aagtcaaaac aaaagaagta ccagagcttg
      841 atgatgagct tgcaaaagat attgatgaag atgttgacac acttgaagac ttaaaagtaa
      901 aatatcgtaa agaacttgaa gcagctcaag aaactgctta tgatgatgct gttgaaggag
      961 ctgcgattga attagcagtt gcaaatgctg aaattgttga tttacccgaa gaaatgattc
     1021 atgaagaagt caaccgttca gtgaatgaat ttatgggcaa catgcaacgt caaggaatct
     1081 cacctgaaat gtacttccaa ttgactggta caactcaaga agatttacat aaccaatatt
     1141 cagctgaagc tgacaaacgt gttaaaacaa accttgttat tgaagcaatt gctaaagcag
     1201 aaggttttga agcaacagat agtgaaattg aacaagaaat taatgacctt gcaacagaat
     1261 ataacatgcc agctgaccaa gttcgttctc ttctttcagc agatatgttg aaacatgata
     1321 ttgcaatgaa aaaagcagtt gaagtgatta caagcacagc aagcgttaag taatcttatt
     1381 aaggaacaaa acgtacaagc cggattagaa cgtctttcta gtctggtttt tctttttcta
     1441 aagaaataat aaggaattag ttagacgtta ttttaatgaa ttagctttaa agatttgtca
     1501 agaagaagtt atatgagttg ataaaagttt tgacgaatta ctttttttag cgtaaaatgg
     1561 tagagaatga taaaataagg agaactgcct tgaaattaga cgtatttgca ggacaagaaa
     1621 aaagcgagct ttccatgatt gaagtggcgc gtgccatttt ggaagaacgc ggtcgcgaca
     1681 atgagatgta ctttagcgat ttagtcaatg agattcaaaa ttacttagga aaatctgatg
     1741 caggtattcg tcatgcatta ccatttttct atactgactt aaatacggat ggctcattta
     1801 ttcctctagg tgaaaacaaa tggggacttc gttcgtggta tgctattgat gaaattgatg
     1861 aagaaattat tactctagaa gaagatgaag atggcgcaca aaaacgtaag aaaaaacgcg
     1921 ttaatgcttt catggatgga gatgaagatg ctattgatta tagagatgat gatccagaag
     1981 atgaagattt cacagaagaa tcagctgagg ttgaatatga tgaagaagat ccagatgatg
     2041 aaaaatcaga agtggagtca tatgattcag aattgaatga aattatccct gaagatgatt
     2101 ttgaagaagt tgatatcaat gaagaagacg aagaagatga agaagatgaa gaaccagttc
     2161 tctaatgtct ttagttcaga aataaaaatg taattatcat ttgacaaatt gtctattata
     2221 gtttatatta ttattcgggc acctctttaa gaggtcaggc aaaagctctc tagttaatag
     2281 ggggctattt ttgttttcta gaataatgcc agtttgcctc tatatttaga aaactaaggt
     2341 gatacctaac caatgtggaa ccaactaata gggaattaac aagaaagcaa tgcttttatt
     2401 cagcttatat aaaggagtac tatgacgaaa tacatttttg taactggtgg tgttgtatca
     2461 tcaattggca aaggaattgt tgcggcaagt cttggccgat tgttaaaaaa tcgtggactt
     2521 aaggtgacga ttcaaaaatt tgatccttat attaatattg atccagggac gatgagcccc
     2581 tatcagcatg gagaagtata tgtgacagat gacggtgctg aaaccgactt ggatttgggt
     2641 cactatgaac gttttattga tatcaatctt aataaatatt ccaatgttac aacaggtaaa
     2701 atttatagtg aagtccttcg taaagaacgt aaaggtgagt accttggagc gactgttcag
     2761 gtgattcctc atatcacaga tgcccttaaa gaaaaaatca aacgcgcagc ttcaacaacg
     2821 gactctgatg taattattac tgaggtcggg gggactgttg gcgatattga aagtttaccg
     2881 ttcttagaag ctcttcgtca gatgaaagct gatgttggtt ctgaaaatgt gatgtatatt
     2941 cacaccacct tgttgccata tttaaaggca gcgggtgaaa tgaaaactaa gccaacgcaa
     3001 cattccgtta aagaattgcg tggtttaggg attcaaccga atatgttggt tattcgtacg
     3061 gaagagccag ttgagcaggg gattaaaaat aaattggctc agttctgtga tgtgaattca
     3121 gaagctgtta tcgagtcacg tgatgtagaa cacttgtatc aaattccatt gaacttgcaa
     3181 gctcaatcaa tggatcagat tgtttgtgat catttgaaat tgaatgcgcc acaagctgat
     3241 atgaccgagt ggtcagctat ggttgacaag gtgatgaatt taagaaaaac aacaaaaatt
     3301 gccttggttg gtaaatatgt ggaattgcca gatgcctact tatctgttgt ggaagccttg
     3361 aaacattctg gttatgccaa tgataccgca attgacctaa aatgggtcaa tgctaacgat
     3421 gtgacggtgg ataatgcagc tgacttgttg ggagatgcgg atgggattat tgtaccaggt
     3481 ggttttggtc aacgtggtac agaaggcaag atccaagcga ttcgctatgc ccgcgaaaat
     3541 gatgtgccaa tgcttggtat ctgtcttggc atgcagttga cttgtgttga atttgcccgc
     3601 catgtcctca atatggaagg tgcaaactct tttgagttag agccaagtac taaatacccc
     3661 atcattgata tcatgcgtga tcaaattgat attgaagata tgggaggtac gcttcgtctt
     3721 ggattgtacc catgtaaatt gaaaccaggt tcaaaagcag caatggctta caataatcaa
     3781 gaggtggtgc aacgtcgtca tcgtcaccgt tatgagttca acaacaaatt ccgctcagaa
     3841 tttgaagcag ctggttttgt tttctcaggg gtatcacctg acaaccgctt agttgaaatt
     3901 gtagaattga aagagaaaaa attctttgta gcagctcaat accacccaga attgcaaagc
     3961 cgtccaaatc gtcctgagga gctatacacc gcttttgtta cagctgctat taaaaatagc
     4021 aattagtggt tttaagggta agtttgtggc attctgcttg gctggcagat actgataaag
     4081 attactagct attagaggga tcttttctat aaaaatagca ttagaaaaag aaaaacgtga
     4141 tatttttagg ttagcacttt ctttttcagt catttttttg taaaatactt ggtatgaaaa
     4201 ctattcgaat agcaaagtat ttaggaattc tattcctttt gataactctt attagcgtag
     4261 gtgctagttt ttatttcttt catgttgcac aaataagaga agagaaatcg tttattaata
     4321 ataagaaacg gagtacgaat aatccattat acccagctga acagtctttt gacgctttac
     4381 cttacgaaaa acgtcaacta acaaatcgtg ggttaaaaca agtggggtgg tacttaccag
     4441 ctgctcaaaa aacaaaaaag acagctattg ttgttcatgg ttttacgaat gacaaagaag
     4501 atatgaagcc atatgccatg ctttttcatg atttgggcta taatgtctta atgccagaca
     4561 atgaggccca tggggaaagt gaagggaact tgattggtta tggctggaat gaccgcctta
     4621 atgtcatggc ttggacagac caactgatta aggaaaaccc tgaaagccaa atcacactct
     4681 ttggcttatc tatgggtgct gcaacagtaa tgatggcaag tggtgagcga ttgcctgcgc
     4741 aagtcacctc cctcatcgaa gattgcggtt ataccagtgt ttgggacgaa ttgaagtttc
     4801 aggccaaggc tatgtacaac ttgcctgcct ttcctttact ctatgaagtc tctgccttat
     4861 ctaagattcg agcaggtttt agttacggag aagcgagctc agtgaaacag ctggctaaaa
     4921 ataaacgtcc aactttattt atccatggtg ataaggatga ttttgttcct acaaaaatgg
     4981 tttatgacaa ttataaggcc acgaaaggtc ctaaggaaat cttgattgtt aaaggggcaa
     5041 aacacgccaa atcctttgaa acaaacccag aacaatacca gaaaaaaatt gccgcttttt
     5101 tgaaaaaagt tgagaaataa gtattgacag ttaaactcac agttgctata ataatttatg
     5161 ttgctgatga gtagcgggga tggcggaatt ggcagacgcg caggactaag gatcctgtga
     5221 ccgctttagg tcgtgtgggt tcaagtccca ctctccgcat aatgtaagag gtgaatcatt
     5281 gattcgtctc tttttttggt taaatgaagg ataagaaggc atagatttac tggagaaaac
     5341 ttttgataat ctgcttttgg aaaacgcttt aagcttgtga aagactagaa atttatggaa
     5401 aattgtgcta gaatgaacaa tgtaaacggg gagttatccc gaaaaataag aggaggcctt
     5461 acaaatggca atcgtttcag cagaaaaatt tgtccaagca gctcgtgaaa acggatatgc
     5521 tgttggtgga tttaacacaa acaacttaga gtggactcaa gcaatattgc gtgcagcaga
     5581 agctaaacaa gctccagttc ttatccaaac atcaatgggt gcagcaaaat acatgggtgg
     5641 ttataaagta tgtcaatctc ttattactaa ccttgttgag tcaatgggta tcactgttcc
     5701 tgttgctatc caccttgatc atggtcacta cgaagatgct cttgagtgta tcgaagtagg
     5761 ttacacttca atcatgtttg acggttcaca ccttccagtt gaagaaaacc ttgctaaaac
     5821 tgcagaagtt gttaaaattg cacatgctaa aggcgtttca gttgaagctg aagttggtac
     5881 aatcggtggt gaagaagacg gtatcatcgg taaaggtgag ctcgctccaa tcgaagatgc
     5941 taaagcaatg gttgaaaccg gaattgactt cttggcagct ggtatcggta acatccacgg
     6001 tccataccca gaaaactggg aaggtcttgc tcttgaccac ttagaaaaat taacagcagc
     6061 tgtaccaggt ttcccaatcg tattgcacgg tggttctggt attcctgacg atcaaatcaa
     6121 agaagcaatc cgtcttggtg ttgctaaagt taacgttaac actgaaagtc aaatcgcttt
     6181 ctctaacgca actcgtgaat ttgctcgtaa ctacgaagct aacgaagcag aatatgatgg
     6241 taaaaaatta tttgacccac gtaaattctt ggctccaggt atgaaagctg ttcaaggtgc
     6301 tgttgaagaa cgtatcgatg ttttcggatc agctaacaaa gcataattta gagtattaaa
     6361 caccctaaag ccctcttgag ggctttttta gatataattg acaccctaaa atggatacca
     6421 gtttctgtga aataatcttt gattgtgaac aaacaaagca taatttagag tattaaaatc
     6481 tggaccctgc tatctatggt aagtcttttt gttattgtta aagaggttaa gcgtaatgtt
     6541 aatatgtttt agctaaaaaa tgtagtgaaa aatgagtacg tagactattg taatagtaat
     6601 tccgtaaaat tgttgaaaaa gaaaatggga aataccttgt caaattaagc accctatgat
     6661 agaatagata ggtatgtggt gctagcacat ccgtaaacat attctacgta ggaggaaaca
     6721 gatcaaaatg gctaaagtat gttattttac aggacgtaaa accgtttctg gaaacaaccg
     6781 ttcacacgcg atgaaccaaa caaaacgtac agttaaacca aaccttcaaa aagttactat
     6841 ccttgttgat ggtaaaccta aaaaagtttg ggcttctgct cgtgcgctta aatctggtaa
     6901 agtagaacgc atctaataga aataagcctt agggcttttt ttattagctt atttctatcc
     6961 ataagctagt cagggtaata aacgctatcg ttaactattc tatctctttt acattacttc
     7021 taaaatatga taaaatagga tgataaaaac tttttgaggt agtaactatg actgtaaaaa
     7081 ttaatacaaa agatggtcta atcgagctat ccgacgatgt gattgcaact gtcgttggag
     7141 gatcagcaac ggaaattttt ggtgttgttg gaatggccag caaaagtgca ataaaagaca
     7201 attttcaatc actacttcgc aaagaaaact acgctaaggg tgttgtagta aaatcaacag
     7261 atctgggtat ttctgttgat gtctacactg tgatgagtta tggtgtgaaa atcagtgaag
     7321 tctctaaaaa tatccaagaa cgcgtgaaat ttaaccttga aagtcagctt ggtctcactg
     7381 ctgatatggt caatgtttac gtgcaaaata ttaaggttgt aggagaaaat tagtgtcaaa
     7441 tattacaaca agcttattcc aagaaatggt ccaagcggca gcgactcgcc ttggcaaaca
     7501 agcagaatat gttaattcct taaatgtttt tcctgttcca gatggtgata caggaacaaa
     7561 catgagcatg actatggaca atggtgccaa ggaagttgct gataaacctg cgtcaactgt
     7621 gggagaagtt ggacaaatgt tatcaaaagg tcttttgatg ggagcgcgtg gaaattctgg
     7681 agttattact tcacaacttt ttcgtggttt tggtcaaagc attaagggaa aagatgagct
     7741 gacaggtaaa gatttggcac aagcctttca agtgggtgta gaagttgcct acaaagcagt
     7801 gatgaaacct gttgaaggaa caattttaac tgtttctcgg ggtgcagcga ctgctgcctt
     7861 aaaaaaagca gaccttacag acgacgccgt ggaagtcatg caagctgctt tagatggtgc
     7921 caaaggtgcc cttgctaaga ctcctgattt acttcctgtt cttaaagaag ttggtgtggt
     7981 tgattcaggc ggtcaaggtc ttgtctttat ctatgaaggt ttcctatctg ctttgaatgg
     8041 cgactatgtg acttcagcag actttaaagc gactcctgct aacatgtctg aaatgattaa
     8101 tgctgagcat cacaaatccg ttgttggtca tgtggctact gaagacatta cctacggtta
     8161 ttgtactgaa atcatggttg cccttaaaca aggcccaacc tatgtgaaag aattcaacta
     8221 tgatgagttt caaggctacc ttagtggcct tggtgactcg ctgttagtgg tcaatgatga
     8281 tgagattgtt aaagttcacg tccatactga agaccccgga cttgtgatgc aagaaggtct
     8341 taaatatggt tcgctcatca agattaaagt ggacaatatg cgtaaccagc atgaagcaca
     8401 ggttcaaaag actgatgtcg aaaaaaacaa ggctgaagta aaagactttg gcttgattgc
     8461 agtagtagct ggagaaggtt tatctgaaat tttcaaggcg caaggtgttg attacgtgat
     8521 ttctggtggt caaaccatga atccatctac agaagatatt gttaaagcta ttgaagcagt
     8581 taatgccaaa caagtcatta tcttgccaaa caataaaaat atctttatgg ctgcccaatc
     8641 tgctgcagaa gtggtggata ttccggctgc tgttgttgca acacgtactg ttccgcaagg
     8701 atttaccagc ttgttggcct ttgatccttc aaaatcttta gaggacaatg tagcagatat
     8761 gtcaactagt ctttctgacg tggtcagtgg aagcgttact ttggcagtcc gtgacaccac
     8821 cattgatggt ttagaaatcc atgaaaatga tttcttggga atggttgatg gtaaaatcat
     8881 cgtttcaaac ccagacatgg aagcaactct aaaagctgcc tttgaaaaga tgattgacga
     8941 agatagcgag attgtgacta tctttgttgg tgaagaaggt gaccaagact tagcagaaga
     9001 acttgctggg tatttaggag agacttacga agatgtggaa gttgagatcc atcaaggaga
     9061 tcagcctgtt tacccatatc tcatgagtgt agaataatag aatacaataa aaaaagagga
     9121 gttaggcaaa ggtctgactc tttttgtcta tattgatgtc gtaatgagtg gttttagtga
     9181 tggtaagccc ctaaaaaatg agggtaatcc atcacaaagc taatggttac gatttaattt
     9241 gacaaaacct agaaaaacat tataattgta ttataacaat aagtcaagag ctgaaaacgg
     9301 ttcagttcta agacattaca aggaggagat tttatgttag aaccatttat ctttattgct
     9361 tttggggtta ttataatcct agctattgtt gccagcaccc tttatgtggt tcgccaacaa
     9421 tctgttgcca tcgtggaacg ttttggtagg taccaaaaaa cagccactag tggtattcat
     9481 atccgacttc cgtttgggat tgacaaaatt gctgcgcgtg tgcagctgcg tcttttacag
     9541 tcagaaatca tcgttgaaac caaaaccaag gacaatgtgt ttgtgacctt gaatgttgcc
     9601 actcagtacc gtgttaacga gcaaaatgta acggatgctt actataaatt aatgaaacct
     9661 gaatctcaaa tcaagtctta tattgaagat gccttgcggt catccgtgcc gaagttgacc
     9721 ttagacgaat tgtttgagaa aaaagatgaa attgccttgg aagtacaaca tcaagtggca
     9781 gaagaaatgt caacctatgg atatattatt gtcaaaacct tgattaccaa ggtggagcct
     9841 gatgctgaag tcaaacagtc aatgaatgaa atcaatgcgg ctcaacgcaa acgtgtagct
     9901 gctcaagagt tggcaaacgc agataaaatt aaaattgtga cagcagcaga agcagaggct
     9961 gaaaaagatc gattgcacgg ggtagggatt gcccaacagc gtaaggccat tgtggatggt
    10021 ttggcggagt ctattcaaga gcttaaagaa gcgaatattt ccttaaacga agaacaaatc
    10081 atgtccattt tgttgaccaa ccaatacctg gataccctca atacctttgc ggcaaaagga
    10141 aaccaaaccc tttttctacc aaatacacca agtggggtag aggatatccg tacacaagtt
    10201 ttatctgccc ttaaaacaaa ataacgtgta aaagcttggc taaaaagttg ctcaatactg
    10261 gatttagcag aaggctatta taccttgtga agtggcaaac gacgaaatga atacattact
    10321 tttattggag aaatgaaagt aaaaaaagcc tttttcatga ttaacaacat agtattgatt
    10381 aaatgttaag ttgtattgac ttttaaatta aataagttta aaatcaactt aataagcaaa
    10441 aaggagattt tcaatgaaat ccaaaaaagt tgttagtgtt atatcactta ccttatccct
    10501 ttttttggtg acaggttgtg ctaaagttga taacaacaaa tcagttaatc gtaagcctgc
    10561 tactaaacaa acctataata gctatagtga tgaccaatta agatcgcgtg aaaataccat
    10621 gtctgtttta tggtaccagc gagctgcgga aactcaagcg ctttatttac aggggtatca
    10681 gttagcaacg gatcgcttaa aagaacaact caataaacca acggataaac cttattcgat
    10741 tgtattagat attgacgaaa cagttcttga taatagccct tatcaagcta aaaatgtttt
    10801 ggaaggaaca ggatttacac ctgaaagctg ggattattgg gtacaaaaga aagaagcaaa
    10861 accggttgct ggtgctaaag actttttgca atttgcagac caaaatggtg ttcaaattta
    10921 ctacatttca gacagatcaa ctactcaagt agatgctaca atggaaaatc tccaaaaaga
    10981 aggtattcca gtacaaggtc gtgatcatct tctattctta gaaaaaggcg taaaatcaaa
    11041 ggagagtcgt cgtcaaaagg tcaaagaaac aactaatgta acgatgctat ttggtgataa
    11101 tcttctagat tttgctgatt tttcaaaaaa atctcaagaa gatagaacag ctttattatc
    11161 agatttgcaa gaagagtttg gaagacgctt tatcattttc cctaatccta tgtatggttc
    11221 atgggaaggt gccatttata aaggtgaaaa gctggatgtg cttaagcaac tagaggaacg
    11281 ccgtaaaagt ttaaaaagct ttaaataaag taattgaaaa agaggtgcaa aagaaaatgt
    11341 aacctctttt taagta
//
LOCUS       DAUELZ010000052        11276 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.053, whole genome shotgun sequence.
ACCESSION   DAUELZ010000052 DAUELZ010000000
VERSION     DAUELZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.053"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            59..1468
                     /locus_tag="VHI09_001077"
     CDS             59..1468
                     /locus_tag="VHI09_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEQ6120960.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHVIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKNLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            1646..3340
                     /locus_tag="VHI09_001078"
     CDS             1646..3340
                     /locus_tag="VHI09_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ6120961.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYQEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVRDVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            3548..4402
                     /locus_tag="VHI09_001079"
     CDS             3548..4402
                     /locus_tag="VHI09_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEQ6120962.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKEGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            4555..5895
                     /locus_tag="VHI09_001080"
     CDS             4555..5895
                     /locus_tag="VHI09_001080"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEQ6120963.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHALANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            5873..6088
                     /locus_tag="VHI09_001081"
     CDS             5873..6088
                     /locus_tag="VHI09_001081"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEQ6120964.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            6088..6960
                     /locus_tag="VHI09_001082"
     CDS             6088..6960
                     /locus_tag="VHI09_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEQ6120965.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNA"
     gene            6953..7780
                     /locus_tag="VHI09_001083"
     CDS             6953..7780
                     /locus_tag="VHI09_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEQ6120966.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVFASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLVKSQTPETLAPHLIQEVVAKAHKEFEKHEKK"
     gene            7767..8237
                     /locus_tag="VHI09_001084"
     CDS             7767..8237
                     /locus_tag="VHI09_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ6120967.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            8258..9919
                     /gene="recN"
                     /locus_tag="VHI09_001085"
     CDS             8258..9919
                     /gene="recN"
                     /locus_tag="VHI09_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEQ6120968.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEY
                     QLLTGDDLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            10091..11023
                     /locus_tag="VHI09_001086"
     CDS             10091..11023
                     /locus_tag="VHI09_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ6120969.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFISQNETDKMFENLTDDRLLNEL
                     DESDEEDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLRSLS
                     DTALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAHYED
                     RKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAVAKRKI
                     ALPQTGEGFSYYPVLLGLMILGLTPIMIPKKINN"
BASE COUNT         3642 a         1886 c         2454 g         3294 t
ORIGIN      
        1 tttttgttaa aataaaaaga aatagttaca ttatttgcat agaagaaagg gatctgttat
       61 ggtttcagtg atgtggtttc gtagagattt aaggattgat gatcaaaaag ctttagctca
      121 tgttattgct agtcaatcac caaccttatg tgtgtttcaa tttaacaagg agcaattaaa
      181 cctgctacat agtcggaatc aatctagttt tattactagc gttttggctt ttagaaagtt
      241 attaaaatgt gaaggtattg acttatatct aatgtatgga gacttaatga cctgttttga
      301 gcaattattg acacaattaa aagactggac agatgtgtat tttaactatg atgaatctgg
      361 atatggtcgt ttacgtgatc aaaaagcagc acaatttttc aaaaaaaatg ggattgccgt
      421 tcatacctac caagatcact atttacatgg tagtcaagag atcattaacc aatcaggtca
      481 gccttataaa gtttttacgc cctactatag gatttggcaa aattatccga aagagactcc
      541 tattaaagta gaactatcgc aaggcaggtg gttaaacctt gagacaccag atgatgtttt
      601 aaggactgtt gagagcttta aagatgaaaa atatcaggat gttgcaactt ttgatgaagc
      661 gtcaaaacaa ctaaatcgat ttattcaaga ccaattagca gcttatcacg ctaatcgaga
      721 ttttccagcg cagctaggga cgagtcggtt gtctcccttt ttaagaatag gagctattgg
      781 cattcgcacg gtatatcacg ctgtgcgtca agcaccaaat agtctagggc aagctacttt
      841 tttaaaagaa ttagcttgga gagactttta caatatggtc tatgtggctt atcctgatca
      901 gaaaactcag cctatccaaa aagccttttc tcaaatcgaa tgggttaata atcctgactg
      961 gtttcagttg tggaaagaag gtaaaacggg ttaccctatt gttgatgctg caatgcttca
     1021 gttacaaaag acaggatgga tgcataatcg tttacgaatg atagtagctt cttttttaac
     1081 taaagatctt ttatgtgatt ggcgtttagg agagcagtat tttcaacaac aactaattga
     1141 ttatgatgct gccagtaata tcggaggatg gcagtgggct gcttccactg gaacagatgc
     1201 tgttccttat tttagaatat ttaatcctgt tactcaaggt aagcgctttg atcccaaagg
     1261 cgagtttatt aaagcatatt tgcctcaact agaacacgtt ccagagaagt atctccacga
     1321 accgtggaag atgccaaaaa accttcaaga gagtgtgtcc tgtattattg gcacagacta
     1381 tcctcaacca attgtcgatc atgctaaaca gcgtgaacaa gctattgcaa agtatgagtg
     1441 ggcaaaagaa aaagcaaaaa tagaatgact gttctcatta taagagttta aacaaaaagt
     1501 tatcatgaat taagattatt taatttaaaa aataagttga ctttatttag attatttgat
     1561 actattcagt tgtctccctt agagaaagcg atttcttgtt ttggagctga tttttaccat
     1621 ttggtcttag aataggatga gtaatatgac ttacaacgag gtatatcaag aatggttgca
     1681 caataatgat cttagtgatg atattaaagc agatttagca gccataaaag acaatgaggc
     1741 tgagattcaa gatcgttttt acaaaacact tgaatttgga acagcagggc taagaggaaa
     1801 acttggagca ggcaccaatc gcatgaatac ctatatggtt ggtaaggcag cacaagcttt
     1861 agctaatact attattgatc atggacctga agcagttaaa aagggcattg cggttagtta
     1921 tgatgttcgc tatcaatcta gaacatttgc agaattaaca tgctctatta tggcagctaa
     1981 cggtattaag gcgtaccttt ataaagggat tcggccaaca ccaatgtgtt cgtacgctat
     2041 tcgtgctttg ggatgtatct ctggtgttat gattacggct agtcacaatc cccaagctta
     2101 caatggttac aaagcttatt ggcaagaagg ttctcaaatt ttggatgaca ttgcggatca
     2161 aattgcacaa cacatggctg cactaactca gtatcaggaa atcaaacaaa tgccttttga
     2221 aaaggctctg gacagtggac ttgttactta tattgatgag agtattgaag aagcatataa
     2281 aaaagaagtg cttggtttaa cgattaatga tactgacatt gataagtcag tccgagtagt
     2341 ctatacccct ttaaatggtg ttgggaatct accagtgcgt gatgttttaa gacgccgcgg
     2401 ttttgaaaat gtttatgtgg tacctgagca ggaaatgccc gatcctgatt ttacgacagt
     2461 tggctatcct aatcccgaag tccctaaaac ttttgcatat tcagaaaaat taggtaaggc
     2521 agttgatgct gatattttga ttgcaactga tccagattgt gaccgtgtgg ctttagaagt
     2581 taaaaatgca gtcggtgact atgtttttct caatggcaat aaaatcggag cgcttttatc
     2641 ttactatatc ttttcacaac gatttgactt aggcaattta ccggctaatc ctgttttagt
     2701 gaaatccatt gtaacaggag acttgtcacg ggctattgct agccattatg gtattgaaac
     2761 cgttgaaaca ttgactggtt ttaagaatat ttgtggcaag gcaaacgaat atgatgtgac
     2821 gaaacagaaa aactatctgt ttggttatga agaaagtatt ggtttttgct atggcacctt
     2881 tgttcgtgac aaagacgctg ttagcgcctc tatgatgatt gtagaaatgg cagcttacta
     2941 caagaaaaag gggcagaatt tactagatgt tttgcagact atttatgcga catttgggta
     3001 ttataatgaa cgtcaaattg ccttggaact tgaggggata gaaggacaaa aacgcattgc
     3061 gaggataatg gaagatttca gacagacacc aatagctagt gttgcagaaa tggcgttaga
     3121 caagacaatt gattttattg atggctatca agattttcca aagcaaaact gtctcaaatt
     3181 ttatttggat gatggttctt ggtatgcact tagaccatca ggaacagagc ctaaaataaa
     3241 attttattta tatactattg gccaaacaca agaaaatagt gcaacaaaat tagatgctat
     3301 tgaagcagcg tgtcgcacaa aaataaatca ggttaattaa acatcaaaaa tggataacac
     3361 ttttacacta tttttccata taaacaacta caaaaggagg tgagactttt aggcaccttc
     3421 tttttcttgt aggtgccact aatcaattta atcgactcaa accaatacta aaaaataagt
     3481 aaaagtataa agaacaggca tctatttttt tgttataata gaaaaatgaa attaaaggga
     3541 gatgtcaatg acagaactaa ttgatggtaa agccttagct caaaagatgc aacaagagtt
     3601 agcagctaaa gtcaacaatc taaaacaaaa aaaaggcatt gtaccaggct tagccgttat
     3661 tcttgtaggt gatgatcctg ctagtcaggt gtatgtccgt aataaagagc gtgcagctct
     3721 tactgtaggt tttaaaagtg agaccgttag attatcagaa ttcatttgtc aagaagagct
     3781 tattgcagta atcgaacgtt acaatgcaga taacactatt catggtattt tagtgcagct
     3841 acccctgcca aatcatatta atgataaaaa aattattctc gccattgatc ccaaaaaaga
     3901 tgtggatggt tttcacccga tgaatacagg tcacctttgg tcaggacgtc ccttgatggt
     3961 tccttgtact ccatcaggga ttatggaatt gcttcgagaa tataatgtta accttgaagg
     4021 taaacatgcc gtcattattg gcagatcgaa tatcgttgga aaaccaatgg cacagctctt
     4081 actggacaaa aatgcaacag tcacgttgac acattcaaga acacgtcaat tagaagaagt
     4141 atgtcgctgt gcagatgtgt tgattgtggc aatcggacaa ggtcatttca taacaaaaca
     4201 atatataaaa gagggtgcga tagtgattga tgtgggaatg aaccgtgatg ataatggcaa
     4261 gctaattgga gatgtggcct ttgatgaggt ggcagaagtt gcagcgaaaa tcacccctgt
     4321 accaggaggt gtcggtccta tgacgattgc tatgttgcta gagcaaactt atcaatctgc
     4381 tctccgtagt actcataaat agttatcatt agacaattca acataagaga atataagaag
     4441 tcagcaatgg cttccttttt tgtttttctg ctcactattt attcagaaaa atggtataat
     4501 tgcattagta tgcaaaggga ctatgtcagg tccaactaag tgaaaggtgg cttgatggca
     4561 gattatttaa ccgtcactca tttgacgaaa tatttaaaat taaaatttga ccgtgaccct
     4621 tatttggaac gggtttatct gactggtcaa gtgtccaatt ttcgaaaacg accaactcat
     4681 caatattttt ccttaaagga cgaaagtgct gtgattcaag ccaccatgtg ggcaggagtc
     4741 tataaaaaac taggattcga cctagaagaa ggcatgaaaa ttaatgtaat tgggcgagtt
     4801 caactttatg aacctagtgg ctcttactct atcgtgattg aaaaggcaga gccagatggt
     4861 ataggtgcct tggctttgca gtttgaacaa ttaaagaaaa aattaacggc agaaggttat
     4921 tttgagcaaa aacacaagca gcctttgcca cagtttgtat caaaaattgg ggtcattaca
     4981 agtcctagtg gtgctgtgat tcgagatatt atcacaaccg tttcacggcg ttttccaggg
     5041 gttgagattt tattattccc gactaaagta caaggtgatg gagccgccca agaagtagtg
     5101 gctaatattc gaagagccaa tcaaagagag gatttggact tgctcattgt cggccgtgga
     5161 ggtggctcga tagaagacct ttgggccttc aacgaggaaa tagtggttca ggctatcttt
     5221 gaatcgcaac ttccagtgat ttcgagtgtg ggtcatgaaa cagacacgac tttagcggat
     5281 tttgtggcag atcgcagagc agccacacca acagcagcag cagagttagc aacgcctatt
     5341 acaaaaacag atcttatgtc ttggatagta gagcggcaaa atcgttccta tcaggcctgc
     5401 ttgaggcgga ttaagcaacg gcaagagtgg gtggataaac tctcgcaatc tgttattttt
     5461 aggcaaccag aacgattata tgatgcctac ctgcaaaaaa ttgatcgcct tagcatgacc
     5521 ctgatgaata cgatgaaaga tcgtctgagt tccgccaaag aaaacaaggt tcagttggac
     5581 catgccttgg ccaatagtca attacagaca aaaatcgagc gataccaaga ccgtgttgct
     5641 accgctaagc gtttactgat ggctaatatg gctagtcaat atgatagtca gctggctcgc
     5701 tttgaaaaag cacaggatgc tttgctatca ctggatgcca gccgaattat cgcccgtggt
     5761 tatgcaatga ttgaaaaaaa tcaagcactg gtagcctctg taagtcagat aacaaagggt
     5821 gatcagttaa ccattaagat gcgtgatgga caattagatg tagaggtaaa agatgtcaaa
     5881 aacgaaaaca tttgaagaaa atttacaaga tttagagact attgtgaaca aacttgaaaa
     5941 tggggatgtc cctttggaag aagctatttc agaatttcaa aaaggaatgc ttctctcaaa
     6001 agaacttcaa aagaccttac aagcggcaga aaaaacactc gtcaaagtga tgcaagctga
     6061 tggcacagaa gtagatatgg atgattaatg gacaaattag ctagaattga cgaagccatt
     6121 cgtcgctact ataagacgac tagtaacggt gtatctgaag agttgattga tgctatcttg
     6181 tattctgttg acagtggtgg caagcgcatt cgaccactta tcttattaga gatgattgag
     6241 ggatttgggg tatcgttaca gaatgctcat tttgatttgg ctgcagccct tgaaatgatt
     6301 catacaggaa gtctgattca cgatgatttg ccagccatgg ataatgatga ttaccgacgt
     6361 ggtagactga ccaatcacaa acaatttgga gaagccacgg ccattttggc gggagatagc
     6421 ttatttttag acccctttgg gttaatagct caagctgaat taaatagcga ggttaaagta
     6481 gccttaatcc aagaactttc cctagcttca ggaacttttg gcatggttgg tggtcagatg
     6541 ctagatatga aaggggaaaa tcaagctttg agtctccctc agttgtcact gattcacctc
     6601 aataaaaccg gaaaattatt aacttttcct tttaaagcag cagctcttgt aacagaacag
     6661 gctattactg ttcgtcaaca actagagcag gcgggaatgc tcatcgggca cgcctttcag
     6721 attagggatg atattttaga cgtgacagct agctttgaag atcttggaaa aacgcctaaa
     6781 aaggatttat ttgcagaaaa agctacttac ccaagtttac tggggttaga agcctcctac
     6841 caactgttga cagagagttt agatcaggct ttgacgattt ttcagacact cgaaagcgat
     6901 gtaggcttta agcctcaaat aattacaaaa ctgatagaag ggttacgact taatgcctaa
     6961 agaaagagta gatgtgttag cctataagca aggattattt gagaccagag agcaagctaa
     7021 acgtggtgtc atggcaggct tagtggtctc tgtgattaat ggccaacgct atgacaagcc
     7081 aggtgacaaa attgacgatg ggactgagtt aaaacttaaa ggtgaaaaac tcaaatacgt
     7141 cagtcgtggt ggattaaaac tggaaaaggg gctgcatgtt tttggtgtat cggttgctaa
     7201 tcaaattggg attgatattg gcgcttcaac aggtggtttt accgatgtta tgttacaaga
     7261 tggggccaaa ctagtctatg ccgtcgacgt tgggactaac cagttggtat ggaaactcag
     7321 acaagatcca cgagtaagaa gtatggaaca gtataacttt cgttatgctc agccagagga
     7381 ctttaatgag ggacagcctg tatttgccag tattgatgtg tcctttattt ctctcagctt
     7441 gattttgcca gccctgcata acgttttgtc agatcaggga caggttattg ctctcattaa
     7501 accgcaattt gaagctgggc gcgagcagat tggtaaaaaa ggcatcgtca aagacaaaca
     7561 gattcatgaa aaagtgattc aaaaggtcat ggattttgcc tcaggttacg gatttacagt
     7621 taagggactt gatttttccc ctattcaggg tggtcatgga aatatcgaat ttttagctca
     7681 tcttgttaag tcacagacac ctgaaacgtt agccccacat ctgattcagg aagttgttgc
     7741 caaggcacat aaggagtttg agaaacatga aaaaaagtga acgacttgaa ttaatcaaaa
     7801 aaatggtttt gacgcatcca attgagacgc aacatgatct cttaagatta ttggcagagc
     7861 atgggttaga attaacccag gctactattt caagagacat gaatgaaatt ggtatagtaa
     7921 agattccttc tggcagtgga cgttacatct atggtctttc tcaagatagt ggaaaaaaga
     7981 tcgttcaagg acctagatcg ataaagagca ctattttagc tgtatctgac aaaacaaaag
     8041 gtttagaaca acacctctat ttaaaagtcg tacctggcaa tagtaaatta atcaaacgtt
     8101 atttattagc agatttttca aaagctattt ttagtcttat tgctgatgat gatagtttac
     8161 tgttaattgc aaagtctcct tcagaagcag atatgatacg ccaagaaatt ttgctttgga
     8221 tgcagggtat aacctagaaa aaatagggga gaaagtgatg ttattagaaa tttccattaa
     8281 aaattttgct attattgatg aaatttccct aaattttgaa aatggtatga cggttttgac
     8341 gggtgaaact ggagcaggaa agtctattat cattgatgct atgaatatga tgcttggtgc
     8401 gcgtgctagt acagaagtga ttcgtcgtgg agctaataaa gcagaaattg aggggttctt
     8461 ttcggtagat gctaccccag aactggttgc gtgtctggaa tcatcaggta ttgctatgga
     8521 agaagaactg attattcgcc gagatatctt tgccaatggc agaagcgtga gtcgcattaa
     8581 tggtcagatg gttaacctag cgaccttaaa acaggtcgga cagtttttgg tagatattca
     8641 tgggcaacat gaccaagaag aattaatgcg accacagctc caccagcaaa ttttggacgc
     8701 ttttggtgat aaggcttttg agcaattgaa agagaactat cagcttattt ttgatcgtta
     8761 taaaagcttg cgtcgccagg ttattgacaa acaaaaaaat gaaaaggaac acaaagatcg
     8821 tattgatatg ttggcctttc agatagcaga aattgaagct gctgccttga gtcgaggtga
     8881 ggacgaccgg ttaaatcaag aacgtgatcg cttaatgaac cacaaacaaa ttgctgatac
     8941 cctgaccaat gcctacgtca tgctagataa tgacgatttt tcaagtctat ccaatattcg
     9001 ctctagcatg aatgacttac tatcaattga gcagtttgat tcagagtaca aagggatgtc
     9061 gacttcgatt tctgaagcct attatattct tgaagaagtg agcaagcaat tatcagatac
     9121 cattgaccaa ctggattttg atggtgggcg attacaggaa attgaatttc gcttggatac
     9181 actcaatagc ttaacccgta aatacggtgg taatgtgaat gatgtgcttg actactacga
     9241 taatattatc aaagaatacc agctattaac aggagacgac ttgtcttcag gggatctaga
     9301 atcagaattg aaagccttag aaaaacaatt ggttgctgca gccactaact tgagtgaacg
     9361 ccgccatcaa ttggcggagc aattagaagc tgagattaaa gctgaactta aggaacttta
     9421 tatggaaaaa gcagatttca aagttcactt taccacatca aaattcaatc gtgatggtaa
     9481 tgaaagcttg gaattttaca tctctactaa tccaggcgaa ggatttaagc cccttgtcaa
     9541 ggtggcttct ggaggagagt tatcacgcct tatgttagcc atcaaggctg ctatttctag
     9601 aaaagaagat aagacgagta ttgtttttga tgaagtagat acaggtgttt ctggtcgcgt
     9661 tgcgcaagcc attgcccaaa aaatttataa aattgggcga catgggcaag ttctcgctat
     9721 ttcacactta cctcaagtta ttgctattgc tgattatcag tattttattt ctaaagaaag
     9781 taaagaagag tccaccgttt ctaaggtaag gttattaact cctgaagaac gtgtggaaga
     9841 aattgctagc atgattgcag gaacagatat gacacaagct gctcttacgc aggctcgtga
     9901 actacttgcc aaacattaag attatgtttg gtatttgata ttttcataat tagatgacat
     9961 tgatttttta ggtgctaaaa taatcattgt gctagaccat ttttcctctg ttaatctggc
    10021 ttggtttgcc ttgacaaacc tctgagaaat agtagaatag ataagagaat tttaagctga
    10081 ggggatagaa atgactagta aaaaagcgtg tttatcaagc atcattgtgt tagcaagttt
    10141 aacgtgtgga aatgatactg ttagtgccaa tcatctctca gcaactggag ataagtttga
    10201 tgattgctca acacttgttg aaaaagatgt ggcccctaaa gatgaacttg agatgttagc
    10261 atggtcctcg tctcaaacaa ctgatgatgc tgacagagac tatgaagatt ttctcgatga
    10321 tgattctttt atttctcaaa atgaaactga taagatgttt gagaatttaa ctgatgatag
    10381 gttattaaat gaattagatg aatcagatga agaagatgaa gaagatacaa ttgagccaga
    10441 gcaaaatgta ataatgccta gtgacgatga gctatttgat ttaactgatg ctgttgagac
    10501 acgccttact gtttctagtg ctccccattt agaggctgaa ttgccgaaac cacatttgag
    10561 gagcctatca gatacagcac tgcggtctgg tgaaattaga ggacatttag ataacaaact
    10621 ggacgctttg tctgtaacag ctacaaagtt agcattaacg atggctcaaa aatttgattt
    10681 gacaacgcat gtctattcta taggtgaaag ctttagtgaa gtattagctg ctcattatga
    10741 agacagaaaa gcagaatcag ctttttctaa gaaaaagaga tttcaccttc ctattgctac
    10801 tccagatgtt gttatagagg agttaaggcg cctagtctct tctattggaa gttcaaaaga
    10861 agatgtttca gttccttata atcggaagct aggtatggca gttgcaaaaa gaaaaatagc
    10921 cctgccacaa acgggagagg ggttctctta ttatccagtt ttacttggtt taatgatatt
    10981 aggattaacg ccgattatga taccaaagaa gataaataat taggtaataa gttattgtag
    11041 ttgtaaaaaa cggggcaaat gttcaagcgt gaaaaggttg acttttgcct cgttttgtat
    11101 ctcgttattg ctaaggcatt ttcttcgagc agctagtgct cgccatacct atctaccgag
    11161 tgattattca atagcactac cagttttgct ttatttagca aggagtgtta gaataaagag
    11221 gtgagaaact agtttagaat ggaaaaatac atgggaacta taaaaattgt tacaga
//
LOCUS       DAUELZ010000053        10887 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.054, whole genome shotgun sequence.
ACCESSION   DAUELZ010000053 DAUELZ010000000
VERSION     DAUELZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10887
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.054"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            57..4088
                     /locus_tag="VHI09_001087"
     CDS             57..4088
                     /locus_tag="VHI09_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HEQ6120970.1"
                     /translation="MVTLGLIFSQLAPPFGTLMALSGHSRSKSPVTEVKADNVSTLKT
                     GSFKLKKFDEDGKTPIKDVTFQLTSETNPSNYKIEQITSGAGDASFANIPPGTYLLKE
                     VAPPSGYQVMADYYRITVSPDGYTQYTYVKVGTTTSSPTTSLPSTSGGGTGGTVFRTS
                     KTSGVVTVTDYNFTTKNKAQGNTDYTTLWATSGEFFDMSFKLKVNEGTQAGDSFTIKL
                     SDYLSPNGIREKFISAPPLMLDKKVVATGIYDESTNSYIYTFNDLINHKQNAEITVNY
                     TFSPEAKKVDRDWYVNTYNITNIIDGQKQDSGNFTIDYGQGQYMTGTLNSGLRLRNNI
                     TYLNRTTGEVEYTIYLNNGASPRDKDFTVKNPVTGRHFLNLEDKSASVAFTQKNITVY
                     RVPLSQKTSKMPYSMSGETDGLESIPFDYSSKGITFTKESFHDNETNSNTAGLLIKIK
                     AYITADNKRSADVSLSAGWTYTNLIRSRSDAKASAFELGNTSSGVANNIEPTVTIRNY
                     KIKKGSIVFTKQDVETKMTLSGVAFRLEKKEENDWQIVEKYKDVQTGTEGKLILSDLD
                     PGEYQLIETKPLDGYLVSSGPVVTFTITDQGTEGTVKPSDKIIPNTKPGKQKIKVIKK
                     DEQSRVPLAGAKFQLESNKGVVLTGETDGKGEYTFTNLPFGDYILTEIAAPKGYILDK
                     TPRSIAIGDTVDKEPEPTVVEAATPRTVRSVSPSATVSDKDVSRNILVKKVEFTTTNG
                     QTPLQVKPNQGENLIARSEFELKKEIDIKKGDYFAVKLSDNIDPFGVSTGETTTFNIT
                     GPYGTLAVGKYDSKSRSIIYTFTDYVEKYEVSNFSTILPYFIDRYAVTRDADINISTS
                     VGSQTNTARVRVLYTPYYGATDSYSPVNIGSMITKLDEKNGTFTNYIYINPMQQFIRN
                     GKLTFQGGGSAIIDNETQVTLFKVNNSTDMPPSWGITDSTLRVENDILVNKKQGEISV
                     DFENILEARNSFIMKVVGKIASSGTSVRTSATLSQKYDNTYGYYSTNGRWIPKGPYSE
                     YYRYDTTAILHSGDSNVDGAIRISVTNRKNSVAFTKTNGLEKPLEATFELRRLNSNKT
                     FTSVKTTKSTKDTGKFSFEGMESGSYEVWETQSPEGYLKPDKAVATFKVDKDGTIKDL
                     TPDNGKIINYPNTAKIIFTKILASEKEELSPATKEKSATFSLWRLKEESLKETNTVSQ
                     AYDEQYYEPVMENNSVRTVTSDSSGNVLFDKLSPGFYAIKEEKAPDGYVKQQGIVRIF
                     QVDSSGKVIKYQYFKDKSIAGKLTEITDLETEQLKQFNEIINKKFVFPMTGGQGIALL
                     MIIGGTMMGIAYFGHRRKQRLND"
     gene            4209..6371
                     /locus_tag="VHI09_001088"
     CDS             4209..6371
                     /locus_tag="VHI09_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEQ6120971.1"
                     /translation="MKTKIFSRLAAVLLVLGSLLPTVVAVAEAESSHKTDVVIHKIKM
                     TSLKGWPKEKNPDGTYTGLGDKNYNGEKIDTITSYFGEGAEELDGVSFTYWSVDKEKY
                     KKLTKNPQNYDTVPKMKAFLQGTEKNKALENSSETIDGKTTGHTADKGGVKVKDLADG
                     YYWFVENSGSNIANGETLSSSAAVPFGLELPVYKADGSTITELHVYPKNTTTKPKIDK
                     NFSKDEKDAALAGGANYDYYQKDKGYVSRIIGSEVSYQIKTEIPAGAQYQTLRWEDTM
                     TKGLTYKAGSLELTITTKGDGKAALNFEFQTDYKLTENQSGFVLKFTESGLEKVKKAV
                     QATKDAKGQVTADGHPMTVDISYKATVNSDAVVDQPDKNTVIFDYSNNPKEHKDPREK
                     STKPKDKQITVEKTWASQSAPTGIAVTYYLYQKGDESGKDKVVDSVTLTTDYKHIFTN
                     LDDSKDYYVKESAIGYTPEYTEAKDGKISIKNTKDDKNPDPLKPTSPAIVTHGKKFVK
                     TSQDDERLKGATFVVVDQKTNEKYLAIKADEKQSAEEKAYHDTEQKYQDEVKKATTEK
                     PNSQAIEKAKKARDDAFKKARTAYEWVSDKQKAVKFTSNSKGQFEVTGLAAGTYYLEE
                     VAAPTGFAKLQEKVEFTLGFNSYNGHKDQSGQSLEGHTQYEKGTPDFGYGQRVINKKI
                     TIPQTGGIGTVIFTVVGLAIMTVTGLMMIRRNKNDKSE"
     gene            6384..7229
                     /locus_tag="VHI09_001089"
     CDS             6384..7229
                     /locus_tag="VHI09_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEQ6120972.1"
                     /translation="MGGTQLKIGRLIRLMLSICACLYFLTSPIFALQKTSSVTIHFEN
                     SDKDTQLALWQLPEGQTLPELETLFEKTDAELTRQYPQVSTVTVPKGETKLVLSNLPV
                     GAIYYVREAEERLGVRSLAPFILKVDTDDDQAVYTKKAKAQKRGSYPFVKVSAQGGSL
                     EGATFEVWKQTQKQLQPVIKGSSRYLLTSSKDGSFMARDLPFGSYVLKEITAPKGYLL
                     SKKTIPFEVTDYSEKQAPVKVVNQPKIPPRIEIPYTGNAIMILVVLLGFALFTLGVYL
                     VRRNG"
     gene            7311..8183
                     /locus_tag="VHI09_001090"
     CDS             7311..8183
                     /locus_tag="VHI09_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEQ6120973.1"
                     /translation="MKKQSKRHRLNQNIALIVVFLIGLTILLYPQISRIYYTIESNYQ
                     SKQFDREKSTLHQEDISQRIALAKAFNASLHDVDLKDPYSDDEKTKGRAEYARMLELH
                     EQIGHVEIPKIRVDLPIYAGTSDEIISKGSGHLEGTSLPVGGENTHTVLTSHSGLPSA
                     KLFSDLAKLKKGDIFYIHNIKEILAYQIDQIKIIEPDNFSDLLIVPGKDYATLLTCTP
                     VGINTHRLIVRGHRVPYVSNEHKNNKQKASSGYMCYLFVLLALLTLLFGYWFYRQKKK
                     KSQKVKREEFHAKE"
     gene            8170..9003
                     /locus_tag="VHI09_001091"
     CDS             8170..9003
                     /locus_tag="VHI09_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEQ6120974.1"
                     /translation="MLKNKQSLSLRERLIQVMFPLLFLMGCLIVLYPLMSNYYYRVKQ
                     NQAVTSFESAKVIVNKDDIKRRMALARAYNATLDPGRINDPYTDLEKKGVAEYARMLE
                     LNEQIGYVEVPRFDINLPIYAGTSDDVLQKAAGHLEGTSLPIGGDSTHTVITAHTGLP
                     QAKLFTNIHKMKKGDLFFIHNIDKTLAYKVDQILVVEPDNFTPVLVKNGFDYATLLTC
                     TPYGINSHRLLVRGYRVPYQKAFEKADAQRPWYTKVIFLVSFLLFVILVIILLIDWHR
                     K"
     gene            9023..9880
                     /locus_tag="VHI09_001092"
     CDS             9023..9880
                     /locus_tag="VHI09_001092"
                     /inference="COORDINATES: protein motif:HMM:NF033745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEQ6120975.1"
                     /translation="MSKQKCVGYVLMILGLGLPLFFLTLMSLNQFQEQVAYQKFQTEN
                     RSWKNSQKEWVNRHNQEQALADRATTDPFVDAQNQLKQSPFDDNIIGYIIIPKLRMAQ
                     PIRVGASERHLEKGVAQVTGTSLPIGGLGTRSVIAGHRSWYDNERFLRIAELSLGDQI
                     VIDLGVYQLEYRVKSVEIIDAKDWRQLTAKKSQDLITLLTCNPLYPPFNERLLVNAER
                     VLPSLSDLPTKTRNKMDQSQLESPLASHKTSLVKLPLVLPFGIWVCLGWLIESYFIYR
                     LFRQCKGDS"
     gene            complement(10222..10881)
                     /locus_tag="VHI09_001093"
     CDS             complement(10222..10881)
                     /locus_tag="VHI09_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120976.1"
                     /translation="MKRCNKYLFTSLLAASMLFSSYKSVHAHDNIDEEGKVHLYWQGN
                     YYVDNYVDYTKKLVNNSIEWTVTFNSAKEQWVYPDFSVFLPKGVKAPKEITYEHHYGN
                     GTVDSKKRSNTQWHYDWEKQKTNFNQEFDKFPGYTGWSRSLDKFYKLKNEGKFSHVLV
                     DTYGRQSHTYFSHKMVWKFKTELEDNYKDKWNKLPFIAGIKQNNPLAASFPSYKGEFG
                     E"
BASE COUNT         3717 a         1875 c         2223 g         3072 t
ORIGIN      
        1 agagagagtt agttatagtg ataaacagaa aaaagaaata taaactaatt agactgatgg
       61 ttaccttagg ccttattttt tcgcagttag cacctccttt tgggacttta atggctttat
      121 caggacattc gaggtcaaaa agtcctgtca cagaagtcaa agctgataat gtgagtaccc
      181 tgaagacagg aagttttaag ctcaaaaaat ttgatgaaga tggaaaaaca cccattaagg
      241 atgtaacgtt tcagttaaca tcggagacca atccgtcaaa ctataagatt gaacaaataa
      301 ctagtggtgc aggtgatgct agcttcgcta atatccctcc aggaacttac cttttaaagg
      361 aagtggcacc gccaagtggc tatcaggtga tggctgatta ctataggata accgtcagtc
      421 cagacgggta cacacaatat acttatgtca aagtagggac aacgactagt agtccgacaa
      481 cctctcttcc ttcgacaagt ggtggtggaa caggcgggac agtctttagg acgtcaaaaa
      541 cctctggcgt agtgacagta actgactata actttacaac taaaaataaa gcacaaggaa
      601 atacagatta tacaacatta tgggcgacat cgggagagtt tttcgatatg tcctttaaat
      661 tgaaggtcaa cgaaggaaca caagcgggtg attcttttac cataaagcta agcgactact
      721 tatctccaaa tggtattcgt gaaaaattca tctcggcacc tcctttgatg ctcgataaga
      781 aagtggttgc cacagggatt tatgatgagt ccaccaatag ctacatctat acctttaatg
      841 atctcattaa tcataaacaa aacgctgaaa taacagtcaa ttacaccttc agtccagaag
      901 ccaaaaaagt tgatagagac tggtatgtaa atacctacaa tattacaaat atcattgacg
      961 gtcagaagca agattctggt aactttacga tcgactatgg tcaaggtcag tatatgacag
     1021 ggacattaaa tagtggctta aggttaagga ataacattac ttaccttaat cggacaactg
     1081 gcgaagttga atataccatt tacttaaata atggagcatc acctagagat aaagatttta
     1141 ctgtaaaaaa ccctgtaaca ggtcgtcatt ttctaaactt agaggataaa tcagccagtg
     1201 ttgcatttac ccaaaaaaac ataactgtct atagggttcc tttaagtcaa aaaacaagca
     1261 agatgcccta cagtatgtcg ggggagacag atggactaga atcaatacca tttgactaca
     1321 gcagtaaggg cattaccttt actaaagaat cgttccatga taacgaaact aatagtaata
     1381 ccgcaggatt attaataaaa ataaaagcat atataacagc tgacaataaa cgatcagcag
     1441 atgtttctct tagtgcagga tggacttata ctaatttaat taggtctagg tcagatgcca
     1501 aagccagtgc ttttgagtta ggaaacacat catctggggt ggccaataat attgagccta
     1561 ctgtgaccat tcgtaactat aaaataaaaa aaggttcaat cgtctttaca aagcaagatg
     1621 ttgaaaccaa gatgactttg agtggtgttg cctttagatt agagaaaaaa gaagaaaatg
     1681 actggcaaat tgtcgagaaa tacaaggatg tccagaccgg aacagaaggc aagttaatcc
     1741 tgtcagactt agatccagga gaatatcaat taattgaaac aaaaccttta gatgggtatt
     1801 tagtttcatc agggcctgtt gtgactttta ccataacaga tcagggaaca gaaggcacag
     1861 ttaagccatc ggataaaatc atcccgaaca cgaagccagg gaaacaaaag attaaagtca
     1921 tcaaaaagga tgagcaaagc agggtaccat tagccggagc caagtttcag cttgagtcca
     1981 ataagggtgt ggtactaact ggtgagacag atggtaaggg agagtatacc tttacaaact
     2041 taccatttgg tgactacatt ttgacagaga ttgccgcgcc aaaaggatac attttggata
     2101 aaacacctcg ctctattgca ataggagata cagtggataa agagccagaa cctacggttg
     2161 tggaagctgc taccccaagg acagtccgat cagtgagtcc ttcagcaaca gtgtccgaca
     2221 aagatgtgtc cagaaatatt ctagtcaaaa aagttgaatt tactacaaca aatggccaaa
     2281 ctcccttaca agttaaacct aatcaaggtg aaaacctgat tgctcgatca gaatttgaac
     2341 ttaagaaaga gattgatatt aagaagggag attattttgc ggttaaactc tcagataata
     2401 tagatccctt tggggtttca acaggagaaa caacaacctt taacatcact ggtccttatg
     2461 gtaccttagc tgttggtaag tatgatagca agagccgaag tattatttat accttcacag
     2521 actatgttga aaaatatgag gtttcaaatt tttcaacgat cttaccatac tttatagatc
     2581 gttatgctgt tactagagat gctgatatta atatctcaac aagcgttggg agtcagacaa
     2641 atactgcaag ggttagagta ctatacacgc cttactatgg agctacagat agttactctc
     2701 cagtaaacat tggatcaatg ataacaaaat tagatgaaaa aaatggaact tttacaaatt
     2761 atatttacat taatccaatg cagcaattta tccgaaatgg taaattaaca ttccagggag
     2821 gagggtcagc tattattgac aatgaaacgc aggtgactct gttcaaagtt aataatagta
     2881 cggatatgcc tccaagttgg gggataactg attcaacctt aagagtagaa aatgatatat
     2941 tggttaataa gaaacaaggg gaaatatctg tagattttga aaatatttta gaggcacgta
     3001 attcatttat tatgaaagta gttggaaaaa tcgcttcctc aggcacatcc gtaaggacaa
     3061 gcgcaacatt atcacagaaa tacgataaca cttacggcta ttattcaaca aatggacgct
     3121 ggattcccaa gggtccgtat tcggaatatt atagatatga tactacagct attttacact
     3181 caggggatag taacgtcgac ggagccatta ggatatcagt gactaatcgt aaaaatagtg
     3241 tagcatttac taaaacaaat ggtctggaaa aaccattgga agctaccttt gagttacgcc
     3301 gattgaattc taataagact tttacttctg tgaaaacaac aaagtcaact aaagatacgg
     3361 gtaaatttag ctttgaaggc atggaatctg gaagctatga ggtttgggaa acgcagtcac
     3421 cggaaggtta tctgaagcca gataaagcag tagctacttt caaagttgat aaggacggta
     3481 caattaaaga tttgacacct gataacggta agattattaa ttatcctaat actgctaaga
     3541 ttatttttac gaaaatattg gcttctgaaa aagaggaatt gtctcctgcc acgaaagaga
     3601 agtctgcaac gttcagttta tggcgactta aagaggagag tttgaaagaa actaatacag
     3661 taagtcaagc ttatgatgag cagtattatg agccagttat ggagaacaat agtgttcgga
     3721 cagtaacctc agatagtagt ggaaatgttt tatttgataa gctttctccg ggtttttacg
     3781 ctattaagga agaaaaggcg cctgatggct acgttaagca gcaaggaatt gtgcgtatct
     3841 tccaagtaga tagttctggt aaggtaatta agtatcagta ttttaaagat aagtcaatag
     3901 ctggtaagct aacagaaatt acagatttag aaaccgaaca gctaaaacaa tttaatgaga
     3961 ttattaataa aaagtttgtt ttccctatga caggtgggca gggtattgca ctattaatga
     4021 taattggtgg tactatgatg ggtattgctt attttggtca tcgtagaaaa caaagattaa
     4081 atgattgatg gttgctattt gaatattgtg gaaaccctcc tttgagagag ggagttgcca
     4141 gtaatttaac aatcaaaaga gtcaaaaaaa catttagata aatatttttt acaaatagga
     4201 gagaacagtt gaaaacgaag atatttagta gattggcagc agtattactg gttttgggaa
     4261 gccttttacc gacagttgta gctgtggcag aagcagaatc atcacataaa accgatgttg
     4321 ttattcataa aatcaaaatg acgagcttaa aaggctggcc aaaagaaaaa aatccggatg
     4381 ggacttatac aggacttggt gataagaatt ataatggtga aaaaattgat acaataacga
     4441 gttattttgg tgaaggtgct gaggaacttg atggtgttag ttttacttac tggagtgtag
     4501 acaaagaaaa gtataagaag ttaactaaaa atcctcaaaa ctacgatacg gtaccaaaaa
     4561 tgaaagcatt cttacaaggt actgagaaaa ataaagctct agagaactct tcggaaacta
     4621 ttgacgggaa gaccactggt catactgctg ataaaggtgg cgtcaaagtt aaggatcttg
     4681 cagatggtta ctattggttt gtcgaaaatt cgggttccaa tattgcgaat ggagagacct
     4741 tgtcttctag tgcggctgtt ccatttggat tggagttacc tgtttataag gctgatggat
     4801 caactataac agagttacat gtatatccta agaataccac aacgaagcct aaaattgata
     4861 agaattttag taaagatgaa aaagatgcag cactagcagg tggagctaat tatgattatt
     4921 atcaaaagga taagggttat gtttcacgta taattggttc agaagtttct tatcaaatta
     4981 aaacagagat tccagctggt gcacagtatc agacccttcg atgggaagac acgatgacta
     5041 aaggtttgac ttataaagct gggagtttag agctaactat cacaacaaag ggtgatggga
     5101 aagctgccct gaactttgag tttcaaacag attataaact aacagaaaat caaagtggat
     5161 ttgtcttgaa attcactgaa tcgggtctag aaaaagttaa aaaagctgtt caagcaacta
     5221 aggacgctaa gggtcaagtt acagctgatg gtcacccaat gacagtggat ataagctata
     5281 aagcgactgt caatagcgat gccgtcgttg accaaccaga caagaataca gttatctttg
     5341 attacagcaa caatcctaaa gaacataaag acccaagaga aaagtctaca aaaccgaaag
     5401 ataaacaaat cactgttgaa aaaacatggg ctagtcaatc agcaccaaca ggtattgcag
     5461 tgacctacta cctttaccaa aaaggtgatg agtctggtaa ggataaggtg gtggattcag
     5521 tcacattgac tacagattat aagcacatct ttacaaactt agatgactcc aaggactact
     5581 atgtaaaaga aagtgccatt ggttacacac cagagtacac tgaagctaaa gatggaaaaa
     5641 tttccatcaa gaacaccaaa gatgataaaa atccagatcc tcttaaacca actagtccag
     5701 caattgtaac acatggtaaa aaattcgtta aaactagtca agacgacgaa cgtctaaaag
     5761 gagccacctt tgttgtagta gatcaaaaaa ctaatgagaa atacttagcc attaaagccg
     5821 atgaaaagca gtctgctgaa gaaaaagcat accacgacac agagcaaaaa tatcaagacg
     5881 aagtcaaaaa agctactaca gaaaaaccta attctcaagc aattgaaaaa gctaaaaaag
     5941 cgcgtgacga tgccttcaaa aaagcaagaa ctgcttatga atgggtaagt gataaacaaa
     6001 aagcagtcaa attcacgtca aatagtaaag gacagtttga ggtaacaggg cttgcagctg
     6061 gaacctatta cttagaagag gtagcagctc ctactggctt tgcaaaactt caagaaaagg
     6121 ttgagtttac acttggtttc aactcttaca atgggcataa agatcaatct ggtcaaagtt
     6181 tggaagggca tactcaatat gaaaaaggaa cacctgattt tggttacgga caacgggtga
     6241 ttaacaaaaa aatcaccatt cctcaaactg gtggtattgg tacagttatc tttactgtgg
     6301 ttggtttggc aatcatgacc gttactggat tgatgatgat ccgtcgtaat aaaaatgata
     6361 aatcggaata gtttgttaga aaaatgggag gtacacaatt gaaaatagga cgattgattc
     6421 gcttgatgct atcgatttgc gcttgtttgt actttttgac gtctcctatt tttgctttgc
     6481 agaaaacgtc atccgtgacg attcattttg agaattctga taaggataca caactagcgt
     6541 tgtggcaatt gcccgaggga caaactttgc ccgaactgga aacactattt gagaagacag
     6601 atgctgagtt aacccgtcag tatcctcaag tatcaactgt cacagtacca aaaggtgaga
     6661 ctaagcttgt attatcaaat ctacctgtag gagcgattta ctacgttaga gaggcagaag
     6721 aacgcttagg agttcgctcg ctagcgccct ttatcttgaa ggttgataca gatgatgatc
     6781 aggccgttta tacaaagaaa gcaaaggccc aaaaacgtgg ttcgtaccct tttgttaaag
     6841 tttctgctca aggtggtagt ctagaaggag caacttttga ggtttggaaa caaacccaaa
     6901 aacagctaca acctgttatt aaaggtagca gtcggtatct cctaacgtcc tcgaaagatg
     6961 gttcttttat ggcaagagat ttgccatttg ggtcttatgt tttaaaagaa atcactgctc
     7021 caaaaggtta tcttttgtca aaaaaaacta taccatttga agtaacagat tattcagaaa
     7081 aacaagcccc tgtgaaagtc gtcaaccagc ccaagatccc tcctagaatt gaaattccct
     7141 atacaggtaa tgcgattatg attttggtgg ttttacttgg ttttgcacta tttacactag
     7201 gtgtctatct ggtacgtagg aacggttaac gaagagacaa ggcactaaga ttactagagg
     7261 tcccgtaagg tgcctctagt tttttaaata aaaggaataa aggtgatagc atgaaaaagc
     7321 aatccaaacg gcatcgttta aatcaaaata ttgctctgat agtagtgttt ttgataggct
     7381 tgactatttt attataccca caaatttcta ggatatacta cactattgaa tcaaactacc
     7441 agtctaagca atttgaccgg gagaaatcaa cgttgcatca agaagatatt agtcagagga
     7501 tagctcttgc aaaggcattt aatgccagtt tgcatgatgt agacctcaaa gatccctatt
     7561 ccgatgatga aaaaacaaaa ggtcgtgcgg agtatgcacg gatgcttgaa cttcatgaac
     7621 aaataggaca tgtagaaatc cctaagataa gggtcgattt gccaatttat gctggaacaa
     7681 gtgatgaaat tatttctaaa gggagtggac atctagaggg aacatctttg ccagttggtg
     7741 gggaaaacac gcatacggta ctgacgtcac attcaggttt gccctctgcg aaactctttt
     7801 cagatttagc gaagcttaag aagggagata ttttttatat ccataacatt aaagaaattc
     7861 ttgcttacca aatagatcag attaaaataa ttgaaccaga taatttttca gatttactga
     7921 ttgttcccgg taaagattac gctacgcttc taacatgtac cccagttggg attaatacgc
     7981 atcggttgat tgttcgtggg catcgtgttc catacgtttc taatgagcac aagaataata
     8041 aacaaaaagc gtcatcaggc tacatgtgct acctgtttgt acttcttgct ttacttacac
     8101 tacttttcgg ttattggttt taccgtcaga aaaaaaagaa gagccagaaa gttaagagag
     8161 aggagtttca tgctaaagaa taagcaatca ttatcgttaa gggaacgact aattcaagtc
     8221 atgttcccct tgttattcct tatgggatgt cttatagtgt tgtatccctt gatgtcaaac
     8281 tattattatc gtgtgaagca aaaccaagca gtaacgtcat ttgaatcagc taaagtcatc
     8341 gttaataaag atgatatcaa acgacgtatg gctctagcta gagcctacaa tgccacttta
     8401 gatccaggaa ggattaatga tccttataca gatttggaaa aaaagggtgt tgcggagtac
     8461 gcgagaatgc tagagcttaa tgagcaaatt ggttatgttg aagtgccgcg ttttgatatt
     8521 aatctcccga tttacgctgg aacaagtgac gacgttctcc aaaaagccgc agggcaccta
     8581 gaaggaacct ctttaccaat tgggggcgat agcacccata ccgttattac agcgcatact
     8641 ggactacctc aagctaaatt gttcacgaat attcacaaga tgaaaaaggg agatttattt
     8701 tttattcata atattgacaa gactcttgct tacaaagtag accagatttt agttgtagaa
     8761 cccgataatt tcaccccagt tttagtgaaa aatggttttg attatgcaac attactaacc
     8821 tgtacaccat acggaatcaa tagtcatcga ttattagtac gcggctaccg tgttccttat
     8881 caaaaagcgt ttgaaaaagc tgatgctcaa agaccatggt atacaaaagt tatttttctt
     8941 gtaagttttc tactatttgt tatattagtg ataattcttt taatcgattg gcatcgcaag
     9001 taatacagaa aggaatagtt tagtgtctaa acaaaaatgt gttggctatg ttttaatgat
     9061 tttgggactt ggtcttccct tatttttctt aacactaatg tcccttaacc aatttcaaga
     9121 gcaggttgct tatcaaaagt ttcaaacgga gaatagatcg tggaaaaatt cacaaaaaga
     9181 atgggttaat cgtcacaatc aagagcaagc gttagccgac agagccacaa cagacccgtt
     9241 tgtagatgca caaaaccagt tgaagcagag cccttttgac gataatatta ttggttatat
     9301 tatcattcca aagcttcgca tggcacaacc gattcgtgta ggtgcaagtg aacgtcatct
     9361 tgaaaaggga gtagcgcagg ttactggaac tagcctaccc atcggcggat tagggacgcg
     9421 atcggttatt gcagggcacc gtagctggta tgataatgaa cgttttctta ggatagcaga
     9481 attatctctt ggagatcaga ttgtgattga tttaggtgtt tatcaattag agtatcgtgt
     9541 taagagtgtt gagattattg atgccaaaga ttggcgccaa ttaacggcta aaaaaagcca
     9601 agatttaata acgttactta cctgtaatcc tctttatccc ccttttaatg aaaggctact
     9661 agtaaatgca gagagagtgt tgcctagcct ttctgattta ccaactaaaa cgcgaaataa
     9721 gatggatcaa tctcaactag aaagtccact ggcgtctcac aagacttcac ttgtcaaatt
     9781 accgctagta ttaccttttg ggatttgggt ctgtttgggc tggttgatag aaagttattt
     9841 catataccgt ctatttcgcc aatgcaaagg tgatagctaa tacttctgac gacgttccct
     9901 cccctctcat tagtttaggt aatattagtt actgcatcgt ttacgttgag ccataaccta
     9961 ttacaagcag attaaagggg tgaaaatgag tctatgacaa aagcttagta gtttggtcat
    10021 atcattaact acaagagacc agagctgctt gttaagtagt tcaaaaaggc agagccttaa
    10081 aagattttct gcctttttaa tcttgttggg cacttacgaa agtatttcgg ccacttcaag
    10141 cataataaaa agagtctgag caatcatgtt gtctcagact ctgactgtat tatgcaaaag
    10201 atgatggact ggcagcttca tttactcacc gaactcccct ttataacttg ggaatgatgc
    10261 tgctaatggg ttgttttgtt taatacctgc tataaacggt aatttattcc atttatcttt
    10321 gtaattatct tcaagctcag ttttaaactt ccaaaccatt ttatgagaaa aataagtgtg
    10381 tgattgacgg ccataggtgt ctactaaaac gtgagaaaat tttccttcgt tttttagttt
    10441 gtaaaattta tctagagaac gactccagcc agtataacca gggaatttat caaattcttg
    10501 attaaaattt gttttttgtt tttcccaatc ataatgccat tgtgtattac tgcgtttttt
    10561 agaatcaact gtaccgttcc cataatgatg ctcataagtt atctccttag gagcttttac
    10621 acccttagga agaaaaactg agaaatcagg gtaaacccat tgttctttag ctgaattaaa
    10681 ggttacagtc cattcgatac tgttattgac caatttttta gtatagtcga catagttatc
    10741 aacatagtaa tttccctgcc agtaaaggtg cactttacct tcctcatcaa tgttgtcatg
    10801 ggcatgtact gatttatacg aactaaacag catcgaagct gctagtagtg aggtgaagag
    10861 atatttatta catcgtttca tatcatg
//
LOCUS       DAUELZ010000054        10731 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.055, whole genome shotgun sequence.
ACCESSION   DAUELZ010000054 DAUELZ010000000
VERSION     DAUELZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.055"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            200..523
                     /locus_tag="VHI09_001094"
     CDS             200..523
                     /locus_tag="VHI09_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6120977.1"
                     /translation="MQFLQKWFKHSKKEKLKESSPLSTEIEPSENWEKIPAYIPADKS
                     DYKKVTLITSAIAAGDRPNSQFKVKRILKRNPEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
     gene            580..975
                     /locus_tag="VHI09_001095"
     CDS             580..975
                     /locus_tag="VHI09_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEQ6120978.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVPVPTEA
                     SPQVEEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VASSAGTITAIHVGQGQVVNPGDGLITIG"
     gene            1020..2141
                     /locus_tag="VHI09_001096"
     CDS             1020..2141
                     /locus_tag="VHI09_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEQ6120979.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIGTELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRMTYKAENVSQMTKILFPIIITLVA
                     GFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQNELVNIISILLGLTISIKMQADLF
                     LNVQTLLIIVFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQ
                     KMATDEDPQNFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            2329..2637
                     /gene="citD"
                     /locus_tag="VHI09_001097"
     CDS             2329..2637
                     /gene="citD"
                     /locus_tag="VHI09_001097"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEQ6120980.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEKTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGVDQYDWKEIDSWNV
                     "
     gene            2625..3512
                     /gene="citE"
                     /locus_tag="VHI09_001098"
     CDS             2625..3512
                     /gene="citE"
                     /locus_tag="VHI09_001098"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEQ6120981.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSVMFDLEDSVSLKEKD
                     TSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDIV
                     DVEAVIERVERENGIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTN
                     MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDG
                     KSVINPRQIPLVNEIYTPTEKEIDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVER
                     AERVIALATAAGVLSEEDI"
     gene            3515..5047
                     /gene="citF"
                     /locus_tag="VHI09_001099"
     CDS             3515..5047
                     /gene="citF"
                     /locus_tag="VHI09_001099"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEQ6120982.1"
                     /translation="MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPG
                     DSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSI
                     ANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDV
                     HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPI
                     SIPQTDVDYVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSF
                     QTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMMELLEEGLVEKILDVQDFDHP
                     SAVSLGKHAEHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVI
                     RGASGGHCDTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINP
                     NRQDLVDHFKSLNVPQFSIEELKEKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVY
                     NV"
     gene            5040..5618
                     /gene="citX"
                     /locus_tag="VHI09_001100"
     CDS             5040..5618
                     /gene="citX"
                     /locus_tag="VHI09_001100"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEQ6120983.1"
                     /translation="MCRDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEAFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            5633..7027
                     /locus_tag="VHI09_001101"
     CDS             5633..7027
                     /locus_tag="VHI09_001101"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEQ6120984.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFI
                     QKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YREVGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLAEQGLADKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLVEGIKEKII
                     GDEAVIACRPADLIEPQMIYLRDEIAQYARSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(7137..8189)
                     /gene="citC"
                     /locus_tag="VHI09_001102"
     CDS             complement(7137..8189)
                     /gene="citC"
                     /locus_tag="VHI09_001102"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEQ6120985.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGEGLLNRIVSHLIDEEYTLGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAVVINANPFTLG
                     HQFLVEKAARENDWVHLLMVSEDRSLIPFSVRKRLIQEGLAHLDNVIYHETGPYLISQ
                     ATFPAYFQKEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDNDWDLLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(8278..8760)
                     /locus_tag="VHI09_001103"
     CDS             complement(8278..8760)
                     /locus_tag="VHI09_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEQ6120986.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
     gene            complement(9460..10530)
                     /gene="xerS"
                     /locus_tag="VHI09_001104"
     CDS             complement(9460..10530)
                     /gene="xerS"
                     /locus_tag="VHI09_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEQ6120987.1"
                     /translation="MRRELLLEKIETYKAIMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
BASE COUNT         3264 a         2120 c         2129 g         3218 t
ORIGIN      
        1 cttttcttgc tatcagtatc ttgatctcag tttttcaaca gttacctaaa aacaaaataa
       61 aaggtaactg ttgaaaaact ttatgaaaca ttgaaagcgc tcttagaaag aacacaaaaa
      121 agccatctca aaaaagattg gtagtatata ataaacttac tagttcctta aataggtaaa
      181 tgtgaaaaag aggtagctca tgcagttttt acaaaaatgg tttaaacact ctaaaaaaga
      241 gaagttgaaa gaatcctcgc cccttagtac agaaatagaa ccttctgaaa actgggaaaa
      301 gattcccgct tatattcctg cagacaaaag tgattacaaa aaggttactt taattaccag
      361 tgctattgct gctggcgata gacctaatag tcaattcaag gttaaacgca ttctcaagcg
      421 caatcctgag gcaatcactg tttctttaat tgcttcaagt atcgctgcag gtgtttaccc
      481 agaaagtcag tttcgggtga catccatcta ttgcaaaagg tagtcggtga acgatacttt
      541 agacgaactc atgacacaat aacatatagg agaaaaatga tgttacgaaa atttaaaatt
      601 accattgacg gtaaagaata tcttgtagaa atggaagaaa tcggtgctcc tgcccaagca
      661 gctgcccccg ctcaaccaat atccactcca gtacctgtcc ctactgaagc aagcccacaa
      721 gtggaggaag cgcaagcacc acagccagta gctgcagcgg gagcggatgc cattccatca
      781 ccaatgcctg gaaccatctt aaaagtctta gtagcagtgg gagaccaagt aactgaaaat
      841 cagccactat tgattttaga agccatgaag atggaaaatg agattgtggc ctcatcagca
      901 ggaaccatta cagctatcca cgtcggtcaa ggtcaagtgg tcaatcctgg tgatggtctc
      961 attacaattg gttaatagta ctataactag aataaataga cagaaaagga agtgaatttg
     1021 tggacacttt aatcgcagga ataacctcca tcaccattcc tcaaattgtt atgatggtca
     1081 tcggtgccct cttaatgtat ctaggaatta aaaaagaata cgaaccaacc ttacttgtcc
     1141 ccatgggact tggaaccatt ctcgttaact tccctggttc aggagtttta acccaagtgg
     1201 ttaacggagt cgagcaagaa ggggtttttg aggctctctt caattttggg attggaacag
     1261 aacttttccc actgttgatt ttcattggta taggagccat gattgacttt ggtcccttgc
     1321 ttcaaaaccc attcatgctc ttgtttggtg ctgcagctca attcggaatt ttctttgttg
     1381 tcgttgttgc cgtactggct ggctttgaca ttaaagaagc agcttcaatc ggcattatcg
     1441 gagcagcaga cggaccgact tctatctttg ttgccaacca actggccaag gatttactgg
     1501 gtccaattac ggttgcagcc tattcttaca tggctctggt tcctattatc caaccatttg
     1561 ccattaaatt agtgacaacg aaaaaagagc gccgtatccg tatgacttac aaagctgaga
     1621 atgtatctca aatgactaaa atcttattcc caatcatcat taccttagta gcagggttca
     1681 tcgccccaat ttctctacct ttagttggct ttttgatgtt cggtaactta ttacgggagt
     1741 gtggtgtgct cgatcgcctg tcacaaactg ctcaaaatga attggtaaac attattagta
     1801 tcttgcttgg attgaccatc tctattaaaa tgcaggcaga cctgttctta aatgtacaga
     1861 cactcttaat catcgtcttt ggtttacttg cttttatcat ggattctatc ggtggggtca
     1921 tgtttgccaa atttctcaat ctcttccgaa aagagaagat taatccaatg attggcgctg
     1981 cgggtatttc agcttttcca atgtcaagtc gtgtcattca aaaaatggca acagatgaag
     2041 acccgcaaaa ctttatcctc atgtacgctg taggtgccaa tgtctctggc caaatcgcct
     2101 ctgttatcgc aggtggtttg ttgctagctt actttagcta agaaaggaag gaaaccttat
     2161 gacaattaat atggaccatt tgattagttc ctttgagcta atggcgcttg gtatggcagg
     2221 tgtctttatc gtgttaggca ttctctatct tgtcgcagag atcctgatca aacttttccc
     2281 agttagtaaa taacaatatc atttaagaaa atcaaggagg taaacgcaat ggatattaaa
     2341 caaactgccg ttgctggttc acttgaatca agtgacctaa tgattacagt atcccctaat
     2401 gacgagaaaa ccattacaat aaccctagac agtagtgttg aaaaacaatt tggcaatcac
     2461 attcgtcaac tcattcatca aaccctagtg aatttgaagg tgacggctgc caaagtagag
     2521 gctgttgata aaggcgcttt agattgcacc attcaagcga gaaccatcgc agctgttcat
     2581 cgcgcagctg gtgttgacca atacgattgg aaggagattg actcatggaa cgtttaagaa
     2641 gaacaatgat gtttgtgcca ggtgccaatg ctgctatgct tcgagatgct cctttgtttg
     2701 gtgctgattc cgtcatgttt gacttggaag attctgtttc actcaaagaa aaagacactt
     2761 ctagagctct cgtccatttt gcgctaaaaa cttttgatta ttcgagcgtt gaaacggttg
     2821 ttcgtgtgaa tggtcttgat tcttgtgggg ctttagacat cgaagctgtt gttttggcag
     2881 gtgtcaacgt gattcgtctt ccaaaaacag agactgcaca agatattgtt gatgtggagg
     2941 ctgttattga acgtgtcgaa cgcgaaaacg gcattgaagt tggtcgcaca cgcatgatgg
     3001 cagccattga atcagccgaa ggtgttctaa atgctcgtga gattgccaaa gcttctaagc
     3061 gcttgattgg tattgctctt ggggcagaag actatgtcac gaatatgaaa acgcgtcgtt
     3121 acccagatgg tcaagaatta ttctttgctc gtagcatgat tttacatgct gctcgtgctg
     3181 ctggaattgc tgccattgat actgtttatt ctgatgtcaa taatacagaa ggattccaaa
     3241 acgaagttcg tatgatcaaa cagttaggat ttgacggtaa atcggtcatc aaccctcgtc
     3301 aaattcctct ggtcaatgag atttataccc caaccgaaaa agaaattgac catgccaaac
     3361 aagtcatctg ggcaattcgt gaagctgaaa gcaaaggctc tggcgttatt tccttaaatg
     3421 gaaaaatggt tgataaacca atcgttgaac gtgcagaacg tgtcattgcc ctagcaacag
     3481 cagcaggtgt tttatctgag gaggacattt aaagatggta acaaataaac tcggtagaga
     3541 tattcctcaa ccatacgccg accaatacgg tgtctttgaa ggagaactcg caaatatcaa
     3601 acagtatgac gaatcaagtc gccgtattaa accagttaag cctggagatt ctaaattact
     3661 aggatctgtt cgtgaagcga ttgaaaaaac aggtttaaca gatggcatga ccatttcttt
     3721 ccaccaccat ttccgtgaag gggatttcat tatgaatatg gtcttggagg aaattgccaa
     3781 aatgggcatt aaaaacctgt ctattgcccc aagttccatc gctaatgtac atgagccttt
     3841 gattgatcac atcaaaaatg gtgtggtcac taacatcact tcatctggtc ttcgtgacaa
     3901 agtaggggca gccatctcag aaggactaat ggaaaatcct gtggttattc gctcccatgg
     3961 tggtcgcgct cgtgccattg ctagtgggga tgtccatatt gatgttgcct ttctgggcgc
     4021 cccaagttca gatgcttacg gaaatgttaa tgggacaaaa gggaaagcaa cctgtggttc
     4081 tttgggctat gccatgattg atgccaaata tgcggatcag gtggttatct tgacagataa
     4141 tttggttcct tatcctaata ccccaatcag cattcctcaa acagatgttg actatgtggt
     4201 aacagtggat gctatcggag atcctcaagg aatcgccaaa ggggcaactc gttttacgaa
     4261 aaatcctaaa gaactcttaa ttgcagaata cgcggctaag gtcatcacta attctcctta
     4321 tttcaaagaa ggattctctt tccaaactgg aacaggtggc gcttctttgg cagtaacccg
     4381 tttcatgcgt gaagctatga ttaaagaaaa tatcaaagct agctttgctc ttggtggtat
     4441 taccaatgct atgatggagt tgctcgaaga aggactggtt gaaaaaattc ttgatgtcca
     4501 agactttgat cacccatcag ctgtttctct tggtaagcat gctgaacatt atgaaattga
     4561 tgccaacatg tatgcctcac ctttgagcaa gggtgctgtt atcaatcaat tagacacttg
     4621 tattttatca gcccttgaag ttgatactaa cttcaatgtt aatgtgatga caggatctga
     4681 cggcgtgatt cgtggcgctt ctggaggaca ctgcgacact gcctttgcgg ctaagatgag
     4741 tttggttatt tcaccactta tccgaggacg catcccaact tttgtagatg aggttaatac
     4801 ggtcattaca ccaggaacaa gtgtcgatgt ggtcgtcaca gaagtgggaa ttgccattaa
     4861 cccaaatcgt caagacttag tagaccattt caaatccctc aatgtgccac aatttagtat
     4921 tgaggagcta aaagagaagg cttacgccat cgttggcaca cctgagcgta ttcaatatgg
     4981 tgataaggtt gttgctctta ttgaataccg tgatggtagc ctcatggatg tggtttacaa
     5041 tgtgtagaga cacttatttt tcaggcgaag ctatccaact tagtgatatg ttaagagccc
     5101 gcgaagaaag agcactgcgt cagctgcatt tattaaagga gtacccagaa ggtagcttat
     5161 tatcggtcac catgaatatc cctggaccaa ttaaaacctc tcctaaactt cttgaagctt
     5221 ttgatatagt gattaaggcc attcaaactg ccttagctga cgataagatt tgttaccagt
     5281 tgcgattact gcctacaacg ggttatgagt attacctcat cacaagtcta cctagccgcg
     5341 acctgaagtt aaaaatgata gccttagaga cagagttgcc aataggtcgt ctcatggatt
     5401 tagatgtctt ggtcttgcaa aatgatctgc ctcattcaat tagcagaacc gtattaggag
     5461 gctcccctag gcaatgtttt atctgttcta aagaggccaa agtctgcggt cgcctacgta
     5521 agcacagtgt cgaggagatg cagactgcta tttcaaaatt actccattca tttttcaata
     5581 aagacaacca atcatcgtca tcagataaga caggttgatg aggagaacaa ttatgacgaa
     5641 aatccgtata acggaaacag ttttacgtga tggtcaacaa agtcagattg ccacacgtat
     5701 gacaaccaag gaaatgattc ctattctcga aacgctagac aatgctggtt atcatgccct
     5761 tgaaatgtgg ggaggagcaa cctttgattc ctgcctacgc tttttaaatg aagacccttg
     5821 ggaacggcta agagccatcc gtaaagcagt gaaaaagact aaacttcaga tgctcttacg
     5881 cggacaaaac cttcttggat atcgcaatta tgctgacgat gtggtcagat cctttattca
     5941 aaaatccatt gaaaatggga ttgatattgt ccgtattttc gatgctttaa acgacccacg
     6001 caacttgcaa acagctgttt cagcgactaa aaaatttgga gggcatgctc aagttgccat
     6061 ttcttacacg acaagtccgg tacataccat tgactacttt gttgaattgg cgaaagctta
     6121 ccaagctatc ggagcggact ccatttgtat caaagacatg gctggtgtct taactcctga
     6181 aataggttac caattggtca aatgtatcaa ggagaataca actatccctc ttgaggttca
     6241 tacccatgct accagtggta tttcagaaat gacttactta aaagtagcag aagcaggagc
     6301 tgatattatt gatacggcga tttcctcttt ttcaggtgga accagtcagc ctgccacaga
     6361 gtcaatggcg attgccttga cggatttagg ctttgacact ggcttggata tgcaagaggt
     6421 ggccaaagtt gcagaatatt tcaacactat tcgtgaccac tatcgagaag tagggatttt
     6481 aaatcctaaa gtcaaagata ctgagccaaa aacattgatt taccaagttc caggtggcat
     6541 gctatcaaac ctattgagcc aattagccga gcaaggccta gctgataaat acgaagaagt
     6601 cttagcagaa gtacctaaag taagggctga tcttgggtat ccgccactcg taacgccatt
     6661 atcacaaatg gttggcacac aggccttgat gaatatcatc tcaggggaac gctacaaggt
     6721 agttccaaat gaaattaaag actatgttcg agggctatac ggtcaatcac cagcgccatt
     6781 agtagaaggt atcaaagaga aaatcattgg tgacgaagca gtcattgctt gcagaccagc
     6841 tgacttaatc gagcctcaaa tgatttatct acgtgacgag attgctcaat acgctcgttc
     6901 agaagaagac gtgttaagct atgcgagttt cccacagcaa gccagagatt tcttgggacg
     6961 ccgtgaagat cctttctatg atgttccggt tcaagaagtt actgtacaac tggacattca
     7021 agactaacag ggaatgacta atcctcttaa ccattaatct ccttggtgaa acgcatttgc
     7081 tgtaagaata ataccaaaaa cctacctgat tttgataatc agataggttt tttatcttag
     7141 taatgtttga cagatgaagt ggcttgtatc tttttaataa tgggttgggc ttctagcgaa
     7201 caaaaataat ctaaagacgt tttgggaagg agttttgcca gaaggtccca atcattatct
     7261 ttcaatgcct gcctagccgt tgaggcactg ataggatctt gctgctgatt gatagctttc
     7321 cttggcaaaa tatctaggag gataccagct tgctgcaact gctctgccat aatttcattg
     7381 taaatagcag ttactcggct ggttggctct tctccaacgt atctttttgt aatctgtaag
     7441 gtttgagcaa tctttagaaa aatagcagta tccagcaaag cttggctctt aatcacgtca
     7501 ttgtcttctt tttggaaata ggctgggaaa gtcgcttggc taatcaaata aggacctgtt
     7561 tcatgatata tgacattatc aagatgagct agaccttctt gaatcaacct ctttctcacc
     7621 gaaaaaggaa ttagactgcg gtcttcactg accattaaca gatgaaccca atcattttct
     7681 cttgcagctt tctctactaa aaattgatgc cctaaggtaa aggggttagc gttgataacg
     7741 acagcagcga ctttaccagg tgtttgctca ggcttattaa gtgtcatcag ataatcttga
     7801 aaaccagttt ttttattttc caagaaactg atgtgattgt cgatatggac aatttcagta
     7861 aaaccaagat ctttgaaaaa agcagcagaa gaggtcttcg tataaacaaa aagatgatag
     7921 ttgccaaggg tatattcttc atcaatgaga tgactcacaa tcctattaag aagtccttca
     7981 ccttgatagg cactggatac gcaaagacag cgcaagctat ttccaaagta agaaccggta
     8041 gcaatcacat cattttgcgc attcatgatg gcacaagtat aatctaaatg agcatccaaa
     8101 cgaatacctt cttgatgaag taggttcttt acactagcca ttgtcgtttt atctgaagga
     8161 aagacttttg aaatagtgta gtaaggcatt tcttctcctt atctttaatt ttattgaatc
     8221 ataacataac ttagtaccaa aacaggctga tctatctaaa ggcaactctg ttttttatca
     8281 acgccatttt ttaaaaggaa atcgaatgtg tttgacagac tcaaaagtat cagcgatttt
     8341 ttgattgcgt tccacagtcc gcaagttttc ttggtctaat aagtcttgac ttttttccaa
     8401 aacatcaaca gtaatgtagt agagtttgat caggcttcct atcttatatt taccagattt
     8461 tggagacata gcaaccactt cattgagatt actatgaagc cattttttat gaggcttagc
     8521 aggtatagtg gctactacaa agccaagttg ttccaaatga gcttgtgcct gatcaacagg
     8581 aagattaatc acatcatcta tcgtgcgtag ttgcatctct ttttcatgtt tttgttccat
     8641 acgtttttcg ataattggtc gactgttatc aatcgttttt ccaataattt cagtagtgtc
     8701 aggaacaatt gacatcagtt tcccaactac tcctagagct tttcttattc tctttggcat
     8761 cttttatctc ctcaagtcgt ttctttttat tataacaaaa tgtctaaaaa tagtatgcga
     8821 taaaccttgt tctaaaaggt taaatagatc aattcaagga aaaaatagga ggcgatccga
     8881 caaaaaaaca caaaaattct aaaaaagtca agctatttta gtttagcctt tgtcaatcgt
     8941 atcagttcaa atagaaaata caagttttga caagaattta tttatgtaac aaactgtgtt
     9001 tttgattctt cagtctatct agcgataatg ttatctgatt cttgttttca accaattgat
     9061 gttaaagaag ctgtcttcat gattctctaa tattccttca attttagctt tatattactc
     9121 taagttatca gtaatgacat taagtagttt aacaagaata atcctaaaag ctactttaca
     9181 aaataacgta ttatcatact aaataaaagt actaacatac taaaaacagg cttatctcat
     9241 ctttttgatg agaaccctac tagatttaat ctataaacat cacaaaaaag tcattcaaag
     9301 aagggacaaa gttagcagta atattccttt ttctaagtgg cttgagtagt ttggaaaacg
     9361 gcctgacacc aaactgtcac ggttaatagt agccatctat taaagttaat tttgaagcta
     9421 tttttatctt taatgttaca taaaaacaat tatgtaacat taaaggttat ctaaagcatt
     9481 tttttgctca tcattgacaa tatgggtata aagatccgtg acttgagtag aggaatggcc
     9541 taattgatga ctaactaaca cctgagattt agtagcatca tatagtcttg tcgctagcgt
     9601 atgacgcaat ttgtgaggag tcacacgaat tttaaaatcc tcagagtact tgccaaccat
     9661 cttttctata ctagaagcat ccattcgatt tggaacgcca cggtattctg ttaaaaagaa
     9721 agcaagatcc tgtttttcag ccttgtaacg tctctgacga acagctagat aagactccaa
     9781 ataacctttc gcaaaacctg ctacattgac tgaatcacgt tttccgcctt tgcgaataac
     9841 ttcgataatc atcatattta gattgacatc ttttaaatca agattcactg cttcagacaa
     9901 acgaacacca gaagcaagta gtagagcaat aatagccaaa tccctctcct tatttttacg
     9961 gaaagaagat ttggcacgat tagacaattt ttgctcatat tccttatcca cataatccaa
    10021 gaaagctaga gtttcatcac ctaaaaacag tttctgctta atattttccg ctctggaagc
    10081 cagggtttct tttttctttt ttgttgacac ttttttcata acattccgat aaaaataagg
    10141 ctctccttgg tcattttcaa cctcttcagt caagtacttg taaaggcttg atagggcaga
    10201 caaggtccga ttaatagtgg tttgagaaag cccctctttg gtggaatagg tgttgagaga
    10261 aggtcgttcc cgcaaataaa gcacaaaggc ctctaaatcc ttcttggtaa ggtgctcaag
    10321 cgtgctcaag tcaatatcag caatctttgg cgcctgcgtt aaatcagcat ccatcagcca
    10381 atcaaaaaag cgtttgtatt cttttaaata ctcatataag gtggtaaagc tgtatggaac
    10441 agctaatttg gattggtaat aatctaaaac ataccaaggc atgatagcct tgtaggtttc
    10501 aattttttct agtaataatt cgcgtctcat atcatcataa ttccttaaca ataacaattt
    10561 ttcaaaaatg aattttcgca aattgtctca tgtattttat gccattcatt ctctacaaga
    10621 gcagtatatc acacctataa atatttttca agaaaattac agtttttcgg aaagatgtta
    10681 cgagacagat ctcttttttt acagatacta acgtcacttc ttttagattc a
//
LOCUS       DAUELZ010000055        10647 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.056, whole genome shotgun sequence.
ACCESSION   DAUELZ010000055 DAUELZ010000000
VERSION     DAUELZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.056"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(43..558)
                     /locus_tag="VHI09_001105"
     CDS             complement(43..558)
                     /locus_tag="VHI09_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEQ6120988.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNQEVLSQFRAVDF
                     VKYYFPNYEQPTQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
     gene            639..1247
                     /gene="recU"
                     /locus_tag="VHI09_001106"
     CDS             639..1247
                     /gene="recU"
                     /locus_tag="VHI09_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEQ6120989.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            1234..3399
                     /locus_tag="VHI09_001107"
     CDS             1234..3399
                     /locus_tag="VHI09_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ6120990.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDTSNSTNTDNNGSHPSTDDKKTTH"
     gene            complement(3866..5203)
                     /gene="pepC"
                     /locus_tag="VHI09_001108"
     CDS             complement(3866..5203)
                     /gene="pepC"
                     /locus_tag="VHI09_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEQ6120991.1"
                     /translation="MSALTETFTEQLFAHYEANAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKIENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQY"
     gene            complement(5388..6212)
                     /gene="nadE"
                     /locus_tag="VHI09_001109"
     CDS             complement(5388..6212)
                     /gene="nadE"
                     /locus_tag="VHI09_001109"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEQ6120992.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREEASDQAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            complement(6214..7668)
                     /locus_tag="VHI09_001110"
     CDS             complement(6214..7668)
                     /locus_tag="VHI09_001110"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEQ6120993.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPENFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAMIGGADATSNVRAGKRFDIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVKEFDAIPLLVDIFVKGELVYQLPTLAEIKAYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMTLIDNIRKDTHGKSE"
     gene            complement(7838..9217)
                     /locus_tag="VHI09_001111"
     CDS             complement(7838..9217)
                     /locus_tag="VHI09_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ6120994.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            complement(9386..10303)
                     /gene="trxB"
                     /locus_tag="VHI09_001112"
     CDS             complement(9386..10303)
                     /gene="trxB"
                     /locus_tag="VHI09_001112"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEQ6120995.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDSEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
     gene            complement(10367..10591)
                     /locus_tag="VHI09_001113"
     CDS             complement(10367..10591)
                     /locus_tag="VHI09_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEQ6120996.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
BASE COUNT         3395 a         2203 c         1832 g         3217 t
ORIGIN      
        1 aaaagcaagt atttaaatac caatacccct aaaaatacaa tgctagcttt gtgacatatc
       61 atttagccga tcaaaggtta gcaatagcaa ctgataatgc ggcatatctt ttgctttctt
      121 gagaaaatat tttaagttgg tctcgttttc tgtgtcataa aaaacataag ccccttctgt
      181 cttttctaat aaaaactgat aatattgact aaattgcgtc ggttgttcgt aatttggaaa
      241 gtaatatttt acaaagtcaa cagccctaaa ctgacttaaa acttcttgat ttttttcatt
      301 ccacctatct ccatgcgttt caaacaagaa tatagtagct atctgcaagg gatactcctc
      361 ttttagctca ttagctactt ccaaagccca ttgttcaaaa cccaagttcc ctgtaaagat
      421 aaaccaatcc acaccgtttt ccaaataacc tattaaatct ttacgaatag cctgtttaat
      481 aattgaaact cgtgggtctt tatggtcaaa aatccctatt tcaaagcttc ggtaacctgt
      541 aattagaatc gcagtcatta ggtgttatcc tttcaaaaaa tatgttataa taaaagtgct
      601 taaattttta gcattaacta gataatatta ggagaacaat ggttaattat cctcataatc
      661 ttattcgaca aaaagtaagt tccgttcaaa agcaaacaaa gcaaaataag gtggacttcg
      721 ccaatagagg tatgtccttt gaagctgcta tcaacgcgac aaatgattat tatttatctc
      781 gtcaaatagc tgtcattcac aaaaaaccta ctcctgtcca aattgtcaaa gtggattacc
      841 ctaaacgcag tcgggcaaaa atcgttgaag cgtactttag acaagcttca acgactgact
      901 attgcggcgt ttataaagga cattatgttg attttgaagc aaaagaaacc aggcaaaaaa
      961 cagctatgcc catgaaaaat ttccatttac atcagattga acatatggct tgcgttttac
     1021 atcaaaaagg aatctgcttt gtccttcttc acttttccac actgaaggag acttactacc
     1081 tacctgctca agccttgatt tcattttatc agatagataa tggcagtaaa tcgatgccca
     1141 ttgattatat cagaaaaaat ggctttaagg tagcattcgg agcctttcca caagtccctt
     1201 atttaaacat aattgaacaa aattttttag gcggtgatta caattaaaaa tccaaaaatc
     1261 cttaagtggc taaagtatgt attaagtgca attcttagcc ttattatcct tgttattatt
     1321 attggtggtc ttttgtttac cttctacatt agcagtgctc cgaaactgtc agaagcccag
     1381 ttaaaatcaa caaactctag cttggtttat gacggtaata acaatctgat tgctgatttg
     1441 ggttctgaaa agcgtgaaaa tgtaacagct gatagtatcc ctattaatct agttaatgct
     1501 attacctcaa ttgaagataa acgtttcttt aaccatcgtg gagtagatct ttatcgtatt
     1561 tttggtgctg cctttcataa tctaacgagt cagaccactc aaggggggtc aacgcttgat
     1621 cagcaactca ttaaactagc ctatttttct actaatgaat ctgatcaaac cttaaaacgt
     1681 aaggctcaag aagtttggct tgctcttcaa atggagcgaa aatatactaa acaagaaatc
     1741 ctgacttttt acatcaacaa agtatatatg ggtaatggca actatggtat gctgacagcc
     1801 gctaagtctt attatggcaa ggatcttaag gatttatctt atgcccagct agccctattg
     1861 gctggaatcc ctcaagctcc tagtcaatat gatccttacc ttcatcctga agctgctcaa
     1921 aatcgccgta acgtcgtgtt gcaacagatg tacatggaaa aacatctgac gaaagcagaa
     1981 tatgaaactg ccatcgcaac tcccgtcgct gaaggtctac aatcactcca acagcgctca
     2041 acttatccaa aatatatgga taattatcta aaacaagtta ttgaagaagt caaaaaagaa
     2101 acgaataaag atatttttac cgctggttta aaagtttata ccaatattat ccccgatgcg
     2161 cagcagactc tttataatat ttatcattct ggtgattatg tttactatcc agaccaagat
     2221 ttccaagttg cttcaacgat tgttgatgtg acaaatggtc atgttattgc tcagcttggc
     2281 ggacgtaatc aagatgaaaa tgtttcattt gggactaacc aggctgtttt aactgatcgt
     2341 gactggggtt ctaccatgaa gccaatcaca gcctatgctc ctgctattga atctggtgtt
     2401 tatacttcta ctgctcagtc gactaatgac tcagtctatt attggcctgg aaccactacc
     2461 caattgttta actgggacct tagatataac ggatggatga caatccaagc tgctattatg
     2521 ctatcgcgaa atgtcccagc agtccgagca ctggaagccg caggacttga ctatgctcga
     2581 tctttcttaa gcagtttagg tattaactat cccgaaatgc actactcaaa cgctatctca
     2641 agtaataaca gtagctcaga taaaaaatat ggtgcaagta gtgaaaaaat ggccgctgca
     2701 tacgctgctt ttgcaaatgg tggtatttat cataaaccaa ggtatgtcaa taaagtggaa
     2761 tttagtgatg gtacaagtaa aacttttgat gaaaaaggaa aacgtgccat gaaagaaacc
     2821 acggcctata tgatgacaga tatgttaaaa actgttctca cttatggtac aggtactgct
     2881 gctgccattc ctggtgttgc gcaagctggt aaaacaggga cttctaacta cactgatgag
     2941 gaactagcta aaattggtga aaaatacggc ctttatccag attatgttgg tacattagcg
     3001 ccagacgaaa actttgttgg ctttactaag cgctacgcca tggctgtttg gacaggttac
     3061 aaaaaccgct tgaccccagt atacggatca agtctagaga ttgcatctga cgtttatcgt
     3121 agcatgatga cttacttaac aaatggttac agtgaagatt ggacgatgcc aaatggtctt
     3181 tatcgcagtg gtggattcct ctacttaagc ggaacctatg cgagcaacac cgactatact
     3241 aattcggttt acaacaatct ttacagcaat aacacgacaa cagcttctag ccaaacgact
     3301 tcagatgata ctagtagtag caatgataca agtaattcaa ccaatacaga caacaatggc
     3361 agtcatccat ctaccgatga taaaaagaca actcattaat ctctaacctc tacttattgt
     3421 ctgtacctac cttttattac atatccattt agttactttt aatatctacc ttaagtgata
     3481 atctaaataa aaagcgagtc cacgttctgt gaggactcgc tttttatatg acacaaatga
     3541 cttaccacat acaatataaa acaaaaaaca gaattcttta atcgtattaa agaaaaggta
     3601 caacgttaag ggaattagca actgattttt caaacgttaa cctaaaacaa aatagactta
     3661 tttaaaattg ttataaatag caactaatgc cataagagag tatacatgaa aaaaacacgt
     3721 ttccatatac cgaagacgtg ttttttcata tgtcattttc ttagcataat aatttttttc
     3781 atgttcactt tcttatgcgg agtccaatcc gtttgctgtt cgttttgtcc agcttttaga
     3841 tttcatacag tcgcaaagaa aggaattagt attgtgctaa agcacccatt ggatcccaag
     3901 gagctagtac ttgtggctct ttttcataag ccgctaactc gtcagctgtt aagaattctt
     3961 tacggacaac aatttgatag gtatattcat ccatccaagc atctgaagca acaaagtaac
     4021 ctttgtcacc aaccttctca ccccatgaat tttcaatctt ccattttaac ggttttcctg
     4081 tttcatctaa gtcaacacct gttaaaacca tagcatgtgt catcaaactt tcactataat
     4141 ccaaacgtcc agccttatct tgactaagat tgatatccat actagcttca aaatcgtaag
     4201 tattagttgc caaaatgcct ttttgacgat ctgaaacttg acccacatca gagccaaacc
     4261 aaacggactc accagcctgc atctgcttga tagctagttc tttgaaacgt ttcatatcaa
     4321 ggttgaggta gcgaacctca cgactaccga ccacatttcc tagcatctca acagtgtatg
     4381 atttgccata aggcttatcg gcagttggcg cattaataac agacacataa tcagaaagct
     4441 ttaagccaac aaatttgtcg taaaacgctt gcggtgtcaa acctttctca acatgatagt
     4501 ggtcatcttt atcacggtag gcaaaatcaa aatgacgagg tggtaaacca agggtcatgg
     4561 ccaagaagtt aaaaatttcc tgaagaagct ctgcttttcg atcctcaact tggtctgcct
     4621 tagcacctga cgcaatcagg tcacgaagaa tttgagcatc ttggcgcaat aatttattca
     4681 aatactggtt taattcacga ctgttacttg atgagattga ttctgggtaa acagattttg
     4741 gcaccactcc atacttctca aacaaggaaa ccaccatgtc ccactgacca ccatcttgtt
     4801 gtggaacatc caagagaaac ttaactttac gactggttaa ttcttggtca gctgtagcaa
     4861 tgacctgctc cataaaccag ttagcttttt catacttatc ccaaaagaaa gtatgagctt
     4921 gtgacagttc aaaattttct aacttaaatt ctgtgattaa tttgtgtcgg aaagtgttca
     4981 aggcagcaaa catccagcaa cgaccagaag ccttttggtt agatacctta tctttagtta
     5041 aatcaataga aaacacaaaa tcattgtcca cttcactttg acgggtttct aatgatttta
     5101 gcaaaccatt atgcgtaacg gcattctcaa tagctgaaaa ttttgcattg gcttcgtaat
     5161 gagcaaataa ttgttctgta aatgtctctg ttaatgctga cattattgtc ctcctaaaat
     5221 attaagataa gcttcacctg aaataatatt ggcaagatgg ttattatcca gccattctat
     5281 tctaaaggat tacttctatt atagacctta tttgattttc ttcaagaaaa aagttaggat
     5341 aacctaactt tttttgtttc ctttttttac tctttgaaca tcagctctta tttccaaaaa
     5401 tcatcaaaaa tggtgattgg caggtgacgc ttgtgttgtc ctttatgcca ccatttttca
     5461 atagttgctt gagctacttt ggaaatcagc ttcccttcca aatagtcgtc gatgtcttga
     5521 taggtcaccc ctagggcaac ctcatcagcc aagcctggtt tttgatcttc taaatcagca
     5581 gttggaactt tttcatacaa agcagcatct gctcctaaaa ctttcaaaag agcctttcct
     5641 tgacgtttgt taagcctaaa gaggggaaga atatctgctc caccatcacc aaatttagta
     5701 aagaaaccag tgatgttttc tgccgcatga tccgttccaa taacagcacc ggccatttgg
     5761 ccagcaatgg catattgact gatcattcgc tgacgtgctt taatattacc tttattgaaa
     5821 tcagaaatct caacgcctgc tgcttgtaga gcctctactt gaccgtccac agctgccttg
     5881 atattaatgg taagggtctg atctggtgca ataaaagcca aggctttttg cgcatctgct
     5941 tcatcagcct gaaccccata aggcaagcga acagctataa attggtaagc ttgatcactt
     6001 gcttcctccc gtaactctgc aatggccatc tgggctagct ttcctgcgag ggtagaatcc
     6061 tgacctcccg aaatgcctaa gacataagtt tttaaaaatg agtgcttacg caaataagct
     6121 tttaaaaaat caacggtttt tcgaatttct tcttgtggat caatgctagc cttgacgcct
     6181 aattgtcgaa ttatctcttc ttgtaatgtc atgtcattca ctctttccat gagtatcttt
     6241 acgaatgtta tcaatcaagg tcatcttatt ttgccacaca tctcgtgcca agtctactgg
     6301 gtagtcttga ggatttaaga cacgcttgta ttcatcccaa agcttgtcaa attctttttt
     6361 agcataagcc ttgatttcag ctaaggtcgg tagctggtaa actaattctc ccttgacaaa
     6421 aatatcaact aagagaggaa ttgcatcaaa ttctttaacg gttttcttaa tataggtata
     6481 ggtcggatgg aacatctcaa tctcagttaa ttcattaaca ttaatatcag taaacgtgat
     6541 atagtcgcct tctgatttgc ctttttcacg actggtgatg cgccaaactt gttttttacc
     6601 tggtgtggat actttttctg cattatttga taatttgatc gtatcacgca tgctaccatc
     6661 ttcttgctca atagagacaa tcttataaac agcacctaag gcaggctgat cataggctgt
     6721 gatcagtttg gtcccaacac cccaaacatc aattttggcc ttttgcattt ttaagttgag
     6781 gatggtattt tcatcgaggt cattagaagc atagatctta gcctcggtaa aaccagcgtc
     6841 gtctaactgc tgacggactg tttttgagag gtaagccaag tcaccagagt ccaatcgaac
     6901 acctagaaaa ttaatcttgt cacccatttc tttggctact cggatagctg ttggcacacc
     6961 tactttaagt gtatcatagg tgtcaacaag aaatacacaa tctttatggg tcttagcata
     7021 agccatgaaa gcatcataat cattaccata ggcttgcact aaagcatgag catgcgtacc
     7081 tgaaacaggg atatcaaagc gtttacctgc tctcacatta cttgttgcat ctgctccacc
     7141 aatcatcgct gcgcgggttc cccaaatcgc agcatctagt tcctgtgcac gacgggtgcc
     7201 aaattccaaa aggggttcat cctcaatcac agaacgaata cgagcagcct ttgtagcaat
     7261 caaggtctgg aagttaacaa tgtttaaaag agctgtttcg accaactgac actgacctag
     7321 agggccttct acttgcacaa tcggctcatt agcaaaaacc aaatctcctt ctttagctga
     7381 acggatggtc aattctaatc gtaactcttt aaggtaggtc aaaaagtttt ctggataacc
     7441 cagttcttca aggtaagcca aatctgtttc tgaaaattga aattgctcta ggtactccac
     7501 catgcgctgt aaaccggcaa aaacagcata accattgtta aaaggttcct tgcggaaata
     7561 gacttcaaac accgcatgac gattatgaat gccctgctca aaataaactt gcatcatatt
     7621 aatctgatac aaatcagtat ggagagttaa actatcatct ttatacataa aggggactcc
     7681 tttgaattat tgttactatt ataacatatt ttaaaaagga ctctttggct aggatttaga
     7741 aataacttga gttatcgcta aagtaaaaaa gaactgatcc atcatgacat cctcaaataa
     7801 gaaagagtta atggattcag ttctatatcg ctaattatta tttaaatttg acattgctat
     7861 aaatcccaaa aacaataatc caaatcgttg ctccaatggc gccgatatag gtggattctt
     7921 gtaagaaaag agagataaag acaaaagcaa agaaagcaag ggtcaaaggc gttgtcactt
     7981 tatattttgg catcaaataa ccatctgcca taaagtcctt agattgacgg tacttccagt
     8041 gagcaatcat ggttaaggcg tagatagcaa tgtatacacc tgatgaggag gccgtaatga
     8101 gtgagaacgc atctgcaact cctggtagga tattaatcaa agctgagata ccaaccacaa
     8161 ccgctgaagc aataatggca cgacttggca ccccttgtcg agataaagta ttaatcttta
     8221 agcgattggt caaagcgttt ggagtctcat tagcaatctg atagagatga cgaccggttg
     8281 aataaagggt tgagttaagc gctgacgctg ctgatgtcaa gaccacaaag ttaatcaagg
     8341 ctgctgccca tttaatacca atcaatttga acaccatcac aaaaggagat tcatcaactg
     8401 gtaactgatg ccatggcaca attgccataa tagagaccaa ggctcccaca taaaagatca
     8461 caatacgggt tggaatttct tgaatagcct ttggtagaac ttttcttggg ttagccgtct
     8521 cagaagtggt aattcccaca aattcgatag cttgataagc aaagaaaacc atttggaagg
     8581 ccataaagaa ctttagtttc ccatttggga acatggaaaa atggtcaaaa atattggaaa
     8641 ggctagcatg gcctgtgtga gtttcaaaac ctgtcaatac catgaaaata gctgttgcaa
     8701 tcaaagctaa aatagctaaa atcttaatca tggcaaacca aaactctgtt tccccaaaaa
     8761 cgcgcacagc aattaagttg atcgaactaa gtaagactaa gaaaaccaac tgaatcaacc
     8821 aggctggcca gcttgggaac caaaattgca cataagcacc tactgctgtg atttctgcca
     8881 ttccaataaa aataagggaa atccaatagg atagacccga aaaataaccc cagcctggac
     8941 caatgtattt ggaaataaaa ttgataaagg tgtgttgatc tggatcataa tagagcattt
     9001 caccgatggc acgcatcatc ataaacatga aggcacctgt aatcatatac acaaaaataa
     9061 tagatggtcc tgttaaggca atagagcgtc ctgcccctaa aaagagacct gttccaatcg
     9121 ttcctgcaat cgcaattaac tgcacatgac gattttctaa gccccttacc attccatttt
     9181 ctagttcgtt attgtctgtt tgttctttta tagacattct aaactccatt cactaaaata
     9241 ctatttatta tatccttttt tcagaaaatt ttcaacattt atctaggaaa gatattaagg
     9301 aaaactgttc tttatatttc ttaaaggtct tatgcaattt agctttttcc agggaaagac
     9361 aaggaaaaat gaaggagttt taaccttatg aagggaaact ttcaaggtag tgataaacac
     9421 cttgccctgc aattgcacca tctccaacag ctgtcgtgat ttggcgaagg tctttttgac
     9481 gaacatctcc aatcgcaaaa ataccaggaa tacttgtcct catatgatca tctgtgataa
     9541 tccaaccttc tgaatcggta atttctagat cctttaccat acctgtcaca ggattcatac
     9601 caacatagat aaacacacct ccaaaagcgt ggtcagtgac ttggcctgtt ttcacatttt
     9661 cgattaggac attggagacc ttaatatcat tgccttgaat ttctttgaca actgaatccc
     9721 agataaagtc gaccttatca ttggcaaaag cacgatcttg cagtattttt tgagctctta
     9781 gttgatcacg gcgatgaaca acggttacct ttttagcaaa ctgagtaaga taaatagctt
     9841 cttcaactgc tgagtcacca ccaccaacga ccaaaagatc ctggtcacgg aaaaacgcac
     9901 catcacatac tgcacaataa gaaactcctc gactagtata gtattcttca cctggaacac
     9961 ctagtacacg gtatttagct cccgttgcaa taataaccgt tttagcttca taactagcat
    10021 cttcagtcaa cacacactta tagtcaccaa aattttctat tttttgaaca ataccataaa
    10081 tgttttcaac gttaaacttc tctaaaggtt cgtacatttt catagctaat tctggtccag
    10141 atatatgatc gtatcccggg taattctcta tatcaaaagt gttgttcatc tgaccaccag
    10201 gtgccccttg ttcaataatg gcaacagata aattacttct agcagcgtat aaagcggcag
    10261 tcattcctgc tggtcctgaa ccaataatta atgtatcgta catgattttt ctcctttaat
    10321 ctactcttgc caatatttta gcaatgataa tcccttcttg caatgattag gattttatga
    10381 caaccaaaat actcataaaa gcaaacgata aaattgggat ggttaaaata gcaaccatta
    10441 gcatctcata aagaaaagct tgtctttcaa tcaatgtttt atctcttttg gtttttgctc
    10501 gccataaagt ccatattaaa caaattaaaa tcatagtaag gcttgagaga atgagacctt
    10561 ttacataaag gctaaagatt tctactaaca tatttccacc tagtcattat ttctttccca
    10621 taagtttttc taaaaaacat aacaaga
//
LOCUS       DAUELZ010000056        10309 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.057, whole genome shotgun sequence.
ACCESSION   DAUELZ010000056 DAUELZ010000000
VERSION     DAUELZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.057"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(297..1742)
                     /locus_tag="VHI09_001114"
     CDS             complement(297..1742)
                     /locus_tag="VHI09_001114"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEQ6120997.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYISEREAAGVVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            complement(1924..2634)
                     /locus_tag="VHI09_001115"
     CDS             complement(1924..2634)
                     /locus_tag="VHI09_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEQ6120998.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            complement(2765..3262)
                     /locus_tag="VHI09_001116"
                     /pseudo
     CDS             complement(2765..3262)
                     /locus_tag="VHI09_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(3451..4812)
                     /gene="radA"
                     /locus_tag="VHI09_001117"
     CDS             complement(3451..4812)
                     /gene="radA"
                     /locus_tag="VHI09_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEQ6120999.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            complement(4893..5339)
                     /locus_tag="VHI09_001118"
     CDS             complement(4893..5339)
                     /locus_tag="VHI09_001118"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEQ6121000.1"
                     /translation="MTKIRGFELVSSFTNNDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIIMINSVGVIDADYYGNEANEGHIFAQ
                     MQNIIDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            complement(5449..6216)
                     /locus_tag="VHI09_001119"
     CDS             complement(5449..6216)
                     /locus_tag="VHI09_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEQ6121001.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWAKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            complement(6334..8118)
                     /locus_tag="VHI09_001120"
     CDS             complement(6334..8118)
                     /locus_tag="VHI09_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121002.1"
                     /translation="MKTARFFWFYFKRYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTMTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAIVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQIISQDMVETAARATHIHDFIMSLPRGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            complement(8121..9827)
                     /locus_tag="VHI09_001121"
     CDS             complement(8121..9827)
                     /locus_tag="VHI09_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121003.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPILFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIEPGQMVGIVGATGSGKSTLVQLIPRLFDPQDGQILLGGKPI
                     KILSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            complement(9820..10269)
                     /locus_tag="VHI09_001122"
     CDS             complement(9820..10269)
                     /locus_tag="VHI09_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ6121004.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
BASE COUNT         3058 a         2299 c         1856 g         3096 t
ORIGIN      
        1 gtctctttca gcatatacca aaaaaatatt gtacactaat tgattcataa aaaccatttc
       61 tttaaatcac actaatcaaa acctcaccgt ttgctgtcat tatcacgtaa gcatctcacc
      121 acctctcttt caaatccaat ctccaaaata gaaataaact atactatttt ttactttacc
      181 aaggtagcaa gctcagataa cgataaaaaa gactgaagaa cttaatcttc aatctttcgt
      241 tgtcgtgact ttttaactcc ttaaagcgtt taaaaataaa ggtgaacgat acagccttat
      301 agcatgtttt tgatgtgctc aatggattta tcacgtccca ataaataaat cgtatttggt
      361 aattctgggc catgcatttc accagatact gcaatacgaa taggcataaa gaggtttttc
      421 cctttgattc ctgtttcttt ttgaacagcc ttaatttgtg ggaaaatatt ttctggtttg
      481 aagtcttcat ctgacatagc ttccaactta gccttgaaag cttgcaagac tgtcgaaact
      541 gtttcacctg ccatcacttc tttttcggct tctgttaatt cagggaaatc agaaaagaaa
      601 aggtcagtca atggaataat ctcatctgca gattttaatt gaggtttgta taattctact
      661 agcttctcag ctttttctgt caaacggcct gcctcttcaa ggaatggttt gcagagggca
      721 taaactgttt caaaatccgc atgtttaaga tactcattgc tcatccaatc cattttcttc
      781 tggtcaaagg cagctggcga cttgctaagg cgattttcat caaaaagggc aatcagttgc
      841 tcacgagaga agatttcctc ttctccgcca ggattccagc caagaagggc gatgaagtta
      901 aagacagctt caggcatata accttttttg cggtaatctt caatgaattg gagggtattg
      961 gtgtcgcgtt ttgataattt tttacccgtt tcagaattga tgataagagt catatgacca
     1021 aactctggcg cttcccagcc aagagcttca tagaccatca attgttttgg cgtattggca
     1081 atatggtcat cgccacggat aacgtgagaa atttgcatat catgatcatc aacaacaacg
     1141 gcaaagttat aagttgggta gccatctttc ttttgaataa cccagtcacc accgatgtta
     1201 ccaccttcaa actcgatatc gcctttaacc atgtcagtcc atttgtaaat accggactca
     1261 ttaacagcaa gacggactgt tggaacgaca cctgctgctt cacgctctga aatgtactta
     1321 gctttttcgt cagctgacat accaataaat tcattgatat agcgaggcgt ttcgccagct
     1381 gcttcttggc gttcacgctc tgctgccaat tcttcctcag tcacgtaaga cttgtaggct
     1441 tttccttcag ctaacaattg atcaatatat tgttgataaa gtgctagacg ttcagactgg
     1501 cggtaatttt catgcgtttc tgggctttca tcccaatcca ttcctaacca ttttaggttt
     1561 tcaagttgag aacgttcacc atcttcaaca tgacgtttac ggtcagtatc ttcaatacga
     1621 atgataaaag tacctccgtg acgacgggca taaaggtaat taaatagggc tgtgcgggca
     1681 ttcccgatat gtaaaagccc tgttggactt ggtgcataac gcacacgaat tggtttagac
     1741 atgcttcttc tcctgttaca tatatcactc agaaacctgt ggcacgaaaa acaagcaaca
     1801 aatgatgatt tgagcttgcc aagacctttt cagacttctt ctaaaatctt tcttattata
     1861 gcatatttta cttgcttagg cgaaaaaaat cagccctaaa aagctgattt tatgttattt
     1921 tgactaattt gaactagttg gtagataacg tttaagagtc ccagcaaatt gctcaaggtt
     1981 aagactttgg atataaatct caccatgacc tctaaaggta ttaacaactc cttcacctgt
     2041 accaattgac tgcataaagc cattttctaa atgaatatca taatcaagtt ctctgctcca
     2101 tgctacaaca tgggcattat caatagtcat tgtgccacca tcaagtgtga ttttcttgat
     2161 agatccaaac gagttagcaa gtaaggtacc caagccttca gttgtcatga caaaaagtcc
     2221 gccttgacca ccaaaaagtg cttttccaat attttgacgt tccattttgt actgtgctga
     2281 accatctagg gctagaaagg caccatcatt tagacgatat tgtttttcac ctagttctaa
     2341 agcaacaatc tgtcctggtg tattgggtgc taatgctaat ttaccatctc catttgacat
     2401 agcttgtgta atgaacattg attcaccaga aaccatggaa cggccgatgg ctccaactaa
     2461 ttttcctaaa ccagatccct taccattcaa tttggtattc aaggttacat tctcagtatg
     2521 ataaaccata cttccttgtt gtaagtaaac tgatccccca tgttccagat caatctcaac
     2581 tagtggaaat tgcatgtttt gatcaatcgt aaatctcatt ctattgtcga ccatcttact
     2641 ctccttgttt gtatgaataa cttgatacaa ttattataac gcttccctat ttctttgtct
     2701 aatattttca ataattttta aaaataaaaa agagctgaat tcagctccac aacttggcta
     2761 ctaactattc cattacttgt gtcatctttc cagtatccac atcgtagacg gcaccgttaa
     2821 taacgacgtc atcagagata agagaggagg ctcttatctt agccatatcc tcgcggacac
     2881 tgtcttccac atcttggaag ggaaggaaat cttgccctga aacatccaca cctaagtgct
     2941 catgaatatg cttggcaaag ccttcattag taaaagtctg cgcgccacaa tctgtatggt
     3001 ggagaaccac aatctctctc gttcccattt gctgctgaga aataaccagc gaccgaatca
     3061 tgtcttcggt gacacgtccg ccagcattcc ttaaaatatg agcatctcct agagccagcc
     3121 ccagagcttg ggccacatgt agccgtgaat ccatacaagt cacaatcgct actttagtct
     3181 tgggttttaa gggcaaatga gctgtcccat gcaaggccaa ataagcctaa ttggcagcca
     3241 taaaatgttc aaaataagac atcaataaac tccttttgag aaacagcaga gctatttttg
     3301 ctgttagttt gtcttatctt aacatctttt atggaaaata gctggattaa cctcttagac
     3361 ggccggtatc gttcgaaaag ttaaaaaaac aaatgattgg gatactttct tgacaaattt
     3421 cctcctcatt tgctcttagt ttctgatcct ctagctaaaa actgccttta aaacctgacc
     3481 aaccgtagtc actccgacaa cctcaatacc ttgagggata tcaatgcctt gcaaagcatt
     3541 tttaggagca tagaccttgg taaatccaag ctttgctgct tcattaatcc gttgctcaat
     3601 acgggtcact cgccgaatct cacctgttaa cccgatctcg cctaagaaag cttcttgagg
     3661 gctggttggt ttttccttat aactggatgc gatagccaca gcgactgcca aatcaatggc
     3721 aggttcgtct agtttgacac caccagccga cttgaggtag gcatcttgat tttgtaagag
     3781 taaaccacag cgcttttcta aaactgccat tattaggctt acgcgattga aatcaagtcc
     3841 cgtagtggtt cgtctggcat tgccaaaaac ggttggtgtc actaaagatt gcacctctgc
     3901 taagatagga cgacttcctt ccatcgttac aacgacagcc gatcctgttg ccccatctaa
     3961 gcgttcctct aaaaagactt gactaggatt taagacctct accaagccgc cagactgcat
     4021 ttcaaaaatg ccaatctcgt tagtggagcc aaagcggttt ttcacagccc gcaagatgcg
     4081 aaaagtatga tgcctttccc cttcaaagta gagaacggta tccaccatat gttctaacat
     4141 acgaggccca gccaaggttc cttccttggt gacatgaccc acaataaagg ttgcaatgtt
     4201 gttcgtcttt gcaagctgca tcaattctgc agtgacttct ctcacctgac tgactgatcc
     4261 ttgaacacct gtaatatcag ggctcatgat agtctgaatg gagtcaataa ttaaaaaatc
     4321 tggtttaata ttttcaattt ctgtgcgaat agcttgcata ttggtttcgg catagaggta
     4381 aaattcattg tcaatatcac ccagacgctc actccgcagt ttaatctgct ctgctgattc
     4441 ttccccagac acatagagaa ccgttccctt attagcgagc tgcgtggata cctgcaagag
     4501 taaggtggat tttccaatac ctggatcacc accaattaaa atcaagcttc caggaaccac
     4561 accccctcca agaactctat taaattcaga catgtcagtc tgtgttctgt gataactaat
     4621 attatccaca tcttttagtt taaccggtct ggacttttct ccagctagac taacacgggc
     4681 atttttgact tctttgacct ctacttcttc cacaaaagaa gaccaggccg agcaatttgg
     4741 acagcgccct aaatactttg gcgactggta gccacactcc tgacagataa aggttgcttt
     4801 tttctttgcc atagtaatct ccctccccta gacaagacaa acccactctt aaagaatagg
     4861 tctgctgtca taagcatttt tgttaaatcg ctttactgcc ctgtcgaacc aaaaccacct
     4921 gttcgttcac ctctggcttg gtcaccatca gcaattaaaa aaggcataaa aacaccctga
     4981 acgatcctct cacccacagc taatgtcaca gggtgatcta taatgttttg catttgtgca
     5041 aaaatatgtc cttcattagc ctcattgcca taataatctg catcaatcac acctactgaa
     5101 ttaatcatga taatcccttt ttttcgtgga tttgacgaac gatcatagag gtaaagcacc
     5161 tcaccatctt gcatataggc cttaacccct gttggtacca acttaatctc ccctggggca
     5221 atggttacag cctcagcaac agacaggtca taaccagctg catgagtcgt ctcacgtttt
     5281 ggtagtaaat cattattagt aaagcttgaa acaagctcga aaccacgaat ttttgtcatt
     5341 atgataggta tatcctttca agtcaatcat atcctcttat tatagactat tcgtaaaaga
     5401 atagcaaaaa agcggtcaac tttaaacgga ccacttttaa tcatgccatc acatctcatc
     5461 cccacgataa ctaaagcgga tgtgggtgaa atcattatcc aaatccttgt ctgccataaa
     5521 agctttggca tcttttttca cttgaaccaa gtcaatacct tgcattttgg cagcatagac
     5581 acagccacaa tagcattgac ggtaaatatc atactcctca cacatttcaa cggatctgcg
     5641 ataaccgttg tttttcttga aatcacttgg caggtatttg gtggtgtaaa ccttttgcac
     5701 atcgatgccc acatcattaa tggtttgcga atttttatga ggactgatgg ttagggcact
     5761 ggcaaagtag tcaaaaccta gttccactgc tttttgggcg gtcttgtcta gtcggtaatc
     5821 aaaacagaca cggcaacggt ccccaccttc tggttcctct tctaaacctc gcacttgtcg
     5881 aacgtactca ttggggacat agtccgcttc gagaaactga acagtattgc ctgttttagc
     5941 gttgaactcg gagacaaact gttgtgtcac ataagctctg cgatggtatt cgtccttagg
     6001 gtgaatatta gaattggcaa agtaaaccgt aatgtcagca aactgcgtca ggtattctag
     6061 ggtgtaagtg gagcatgggg cgcagcagac atgcattaaa atactggggc gcacggattc
     6121 cttagcccaa accttggcca tctgttgcat gaccctgtca taatttatct tttgattggg
     6181 attcatgtta gcgagaatct cttgtaaatc aatcattgtg gctactttct aattgttttt
     6241 tctcttgctt gctattatac catatcagga aagaaagctt gactgcttta ttgttcacca
     6301 aaaaactccg ctaagacgcg gagctgatgc catctattca aagacaaatt gattgtgata
     6361 caattcggca taaaagcctt tttgatgcaa tagctcatga tgatttcctt gctcaatgac
     6421 cttgccatct ttcaacacaa taatgtgatc ggcatttaaa atggttttga ggcggtgagc
     6481 aatgacaaag ctggttcgac ctgccacgat agcttccatg gcccgttgaa ttttactttc
     6541 ggtaaccgta tcaacatttg aagtggcctc atccaaaatc aacacttcag ggtccgtcag
     6601 tagcgtccta gcaatagaaa tcaactgctt ttgacctgtt gaaaagacat tgtcatcatc
     6661 tgagacgtag gtattgtacc ctcttggtaa ggacatgata aagtcatgaa tatgggtcgc
     6721 acgcgcagca gtttcaacca tgtcttgact aatgatctga tcaccaaaac gaatattatc
     6781 cgtaatagtt cctgaaaaaa gaactgactc ttgcaacaca atccctacct tttggcgaag
     6841 actatccaaa tcgtagtcac gaatatcacg gccatcaaag gtaatcgaac ctgcatccac
     6901 atcgtagaaa cggttaatca agttcataat agtggtcttt ccagaacctg tcggtccaac
     6961 cacggcaatc attttgccct tgggtgccac gatagataca tccgataaga ctttttgccc
     7021 aggaaggtaa ccaaaatcaa cgtggttgat ggccactgct tcttttaagc tggtgaacgc
     7081 tggtgcattt tgtggacgaa cttcttcggt ttcatcaaac atttcttgaa tacggtgagc
     7141 accggtaaag gccagctgca attctcccca actagacgcg atttgcatca tgggttggta
     7201 atattgctgg gaatattgta caaaagtaac cactaaacca agcgctgccg ctgctggcat
     7261 agatttgtca ctgaggacaa ttgttgaacc gacaaaaatc acgatagccg tgttaataag
     7321 gctcattcca ttcatgactg gaaataattg tcctgagaac agacggcgtt tgaaagtggc
     7381 ttgtcgaacc ctttcattat gctttaaaaa ggctgtcatc gtatcttctt ggacaccttg
     7441 tacaataata gccttttgtc ctgaaatggt ttcatccata aaagcattta aagcactgac
     7501 ttcttgctgt tggatattgg tgtattttct tgccaaacgg atgttaatca ctaaaaaaat
     7561 gagagcaact ggggttgatg cgatggttaa caaagctaaa cggctatctt gcctaaacat
     7621 catccagacc aggccgatgt aaagggcaat attagtcacc acttgaacca aggattggtt
     7681 cagcgagttt tgaatattat ccaaatcact cgtgaaacga gaaagaatct ccccatcttt
     7741 atggcggtca aaaaaggcga cggttaaacg ttctaattta ccaaacaagc ccttgcgcat
     7801 gcggttggtc gaatgtgaga caacacgtgt aagtaagaaa ctatatatta gattagctaa
     7861 aacagtaaag aaataagtca tcaaaagctt aaacatcacg gcattaaaag ctgataaatc
     7921 agggctaaaa tgtgtctgac cactcatctt agcaacgtaa taagcctgac cgatttttcc
     7981 caactcagtc aaggactctc ctaagaagac aggagctttt acttgtaaat aagttgctaa
     8041 gataacggca acagcaatga cagtaaatga gaaacgatag cgtttaaaat aaaaccagaa
     8101 aaaacgtgct gttttcatca ctactcctcc cttccttttt gtgtctcata aatttcacga
     8161 taaatcgcat tattagccac caactctgcg tgtgttcctt ctccaattaa ctgtccttgg
     8221 tcaaggacta agatcttatc tgccttaact accgatgaaa ttttttgagc aacaataact
     8281 gtagttgtac cttctaattt atgacttaag gcttcttgaa cgcgtttttc agattttgca
     8341 tcaagagctg aagtggaatc gtctaatata aggattttgg gatgattaat gacaccacgt
     8401 gcaatagaca gacgttgctt ttgtccgcca gacaagttac tacctcgctc ttcaacttgg
     8461 ctctcataac ggctatccat gcgatcaata aattcttttg cttgcgcgat ttgagcggct
     8521 ttttgcatag catcaatatc agcttttgct gagccttggc gaaggttatc agctatcgtt
     8581 cctgaaaaga gaatagcctt ttgcaacacg atggaaaccg attggcgcag ggttgtttga
     8641 cttaggattt taatcggctt tcctcctaac aaaatttggc catcttgagg atcaaataag
     8701 cgagggatga gctgtacaag cgttgatttc ccagatcctg ttgcaccaac gatgccgacc
     8761 atttgcccag gttcaatagc aaaagaaatg tgcttcaagg ttggctcatc atcattagga
     8821 tatgtaaaac taacatcgtc aaatacaatg cttcctgaga tggtttcttc cttttcattt
     8881 tcaaatgtca tggcaggttc tgtagacaat atttgcctaa ttctagccat agaaacaaaa
     8941 gcacgactaa cttgcatccc catggaacca accacaataa ttgaaaacat aatttgcatc
     9001 atataagtca tgaaagaggc aatattccca atcactgttg gatctgtttc gaccatggtc
     9061 gaaacaacat ttatagaaac ataaactgcc aaataagaca ccaacattaa agccggctgc
     9121 attagtgaaa aaccataacc aatagataaa tttaacgcca aaagatcatt ggacgtttct
     9181 ttaaatttag tgtattgttg ttgttcttgt acaaatgatt taaccacgcg aacgccacgg
     9241 agattttcct tagcgatgcg attaatctta tccattaacc gttgaaattt accaaaacga
     9301 ggacccattt gacgcatcac caaacccatg atgagggcaa ttaagataac cattacaaca
     9361 atgacccacc ataattgagg gaatgtctga actgccataa taaacgcgcc aataaaaagg
     9421 atcggcaaac gaaagagaat ttgaaacatc atcatgacta aactttggat ttgattgata
     9481 tcattagtta gcctaaccac aagattacca gcattaaaag cttcaatatt ggcatatgaa
     9541 aagtcttgga tttttcgaaa ggtcttctcc ctcatatcag cagatacacc ttgagctatt
     9601 ttagcagcta aaaccgtatt aatcgctcca gacattaacc ctaaaagggc aataataagt
     9661 aatggtttcc caacctcata aattttctct tgattatttt gaataacagc gcttagcaca
     9721 gatttaagat aatgtggttg caagagacta cttgcaacaa ccccaataac cataaaaaat
     9781 gagcctaaag cataccattt ataacgtaag attgcgtctt taagcatcgt tgtctccttt
     9841 ataagttagc atattttgct tgagctgttc aattactttt ttcgctgtca gcaactcttc
     9901 ttttgtaatt tcttttaaaa aatagtgctc gatatcctta cgacactccc gcaacaaagg
     9961 caatttatct ctaccaacct gcgctaacac tacttgctta taacgcttat ccacttggga
    10021 aggcatcaca ttgataaacc catttttgac catacgtttc actaaatgac tagcaactga
    10081 ctttgagata cgaagttgtt tttcaatatc tttgacaaat atttcttgat tttggtgttt
    10141 agctaaaaaa acaagaacat aaccttgagg acctgctaga tgctctacat catatttttt
    10201 tgcaatctca tcactaattt gttcgatttg atgtatcaat tcacgtaaat cacctatcac
    10261 ttgtgacatc tcttccctcc ttcttttcct taatagtttc tatgataag
//
LOCUS       DAUELZ010000057        10194 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.058, whole genome shotgun sequence.
ACCESSION   DAUELZ010000057 DAUELZ010000000
VERSION     DAUELZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.058"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..307)
                     /locus_tag="VHI09_001123"
     CDS             complement(<1..307)
                     /locus_tag="VHI09_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEQ6121005.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQTIIAEEVALLKEMLENITRR
                     MIGDDAFTVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     "
     gene            466..2265
                     /gene="pepF"
                     /locus_tag="VHI09_001124"
     CDS             466..2265
                     /gene="pepF"
                     /locus_tag="VHI09_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ6121006.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALQDIDLFKRN
                     YEGRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVSYENFYQNHENAEIREKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYASVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNDLNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVMSTAGYLNLKNNPDGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIINYTEEMSHA"
     gene            2258..2737
                     /locus_tag="VHI09_001125"
     CDS             2258..2737
                     /locus_tag="VHI09_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEQ6121007.1"
                     /translation="MPNLYDFTVKAQNGNDLSLAAYKEKVVLIVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFVKIKVNGKD
                     ADPLFAWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(2783..3166)
                     /locus_tag="VHI09_001126"
     CDS             complement(2783..3166)
                     /locus_tag="VHI09_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEQ6121008.1"
                     /translation="MNKRFKTGFLALVAMLLVACSQGTKQTQTPSVPKADHHVRLVVK
                     EDTNTIDEKVSFGKGDTVLEILKDNYEVKEKDGFITAIDGIEQDTKANKYWLFKVNGK
                     MADKGADQITVKDGDSIEFYQEVFK"
     gene            complement(3150..3653)
                     /locus_tag="VHI09_001127"
     CDS             complement(3150..3653)
                     /locus_tag="VHI09_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ6121009.1"
                     /translation="MSAKLMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFALVLLLWRFVLYPLSQQFPKYQLGCQ
                     AFLAALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
     gene            3978..4826
                     /locus_tag="VHI09_001128"
     CDS             3978..4826
                     /locus_tag="VHI09_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEQ6121010.1"
                     /translation="MRRRIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKTSTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARTFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     regulatory      4953..5049
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5094..5657
                     /gene="thiT"
                     /locus_tag="VHI09_001129"
     CDS             5094..5657
                     /gene="thiT"
                     /locus_tag="VHI09_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEQ6121011.1"
                     /translation="MLPNTNVKYLIEAAIFAALAMTLSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     gene            5750..6229
                     /locus_tag="VHI09_001130"
     CDS             5750..6229
                     /locus_tag="VHI09_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEQ6121012.1"
                     /translation="MTKKTKLKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            6305..6928
                     /locus_tag="VHI09_001131"
     CDS             6305..6928
                     /locus_tag="VHI09_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ6121013.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKPDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNASLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(7036..7938)
                     /locus_tag="VHI09_001132"
     CDS             complement(7036..7938)
                     /locus_tag="VHI09_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEQ6121014.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDVNDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            8112..9605
                     /gene="lysS"
                     /locus_tag="VHI09_001133"
     CDS             8112..9605
                     /gene="lysS"
                     /locus_tag="VHI09_001133"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEQ6121015.1"
                     /translation="MSNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            9741..9881
                     /locus_tag="VHI09_001134"
     CDS             9741..9881
                     /locus_tag="VHI09_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121016.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(9978..10190)
                     /locus_tag="VHI09_001135"
     CDS             complement(9978..10190)
                     /locus_tag="VHI09_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEQ6121017.1"
                     /translation="MAEFTFKIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
BASE COUNT         3159 a         2056 c         1875 g         3104 t
ORIGIN      
        1 aatcgacatc ttctgaaata ttgattagca agggtaaaat agaaaaatac ctggaaatca
       61 cttccatttc ttgatttgaa atattggcaa caactttttc taattctatg taatcttgtt
      121 tttcagataa caccataata gactcgataa ctgtaaacgc atcatctcca atcatccgtc
      181 gtgtgatatt ttccaacatt tctttcaaaa gagcaacttc ttcagcaatg atagtttgat
      241 tgttactact ctctaacttt ttaagtggca aatgaccatc tccttctttg ctagtttctt
      301 ctatcatacc actttttacc aaaaataaaa gtgatcatgt ccataacgtt tgttttttaa
      361 aaaaagtaag cgctttattt taaagaatgt ttggataggt ttagtctcca tattccacta
      421 aaattctgtt atactagtct tgactatatt gttaaggaga caactatgga attgaaaaaa
      481 cgttcagaat tccctgaaaa tgaactttgg gatctaacag cgctctataa agaccgccaa
      541 gattttttac tcgctattga aaaagcctta caagatattg acctgtttaa gcgcaattat
      601 gaaggccgct taacatcggt ggacgatttc actcaggcct taatagaaat tgagcatatt
      661 tatattcaaa tgagtcatat tggcacttat gcctttatgc ctcaaaccac tgacttttca
      721 gatgaaagtt ttgctcaaat tgctcaggct ggcgatgatt tcatgactaa agctagcgtt
      781 gcccttagct tctttgacac cgccttagcc aatgccgacc ttgacgtgtt agatacatta
      841 gaaaagaacc cttatttcag cgcagctatt cgcatggcaa aaatccaaaa agaacacctc
      901 ttaagtcctg atgtggaaaa ggctttggct aacttacgcg aggttatcaa tgcgccctat
      961 gatatttaca ccaaaatgcg ggctggtgac tttgacatgg aggattttga ggtggatggt
     1021 aaaacctata aaaatagctt tgtttcttac gaaaatttct atcaaaatca cgaaaatgct
     1081 gagattcgtg aaaaagcctt ccgctctttt tcaaaaggtc tccgtaaaca ccaaaataca
     1141 gctgcagcgg cttacttagc caaggttaaa tctgaaaaac tgttagcgga tatgaaaggc
     1201 tacgcttctg tctttgatta cctcctagct gaacaggaag tggatcgtag cttgttcgac
     1261 cgccaaattg atctcatcat gactgaattt ggaccggttg ctcagaaatt cttaaaacat
     1321 gtcgcccaag tcaatggtct tgagaaaatg acttttgctg actggaaact agatattgac
     1381 aatgatctca atccagaagt cagcattgat ggcgcttatg acctcgtgat gaaatctcta
     1441 gccccattag ggcaagaata cacgaaagaa atcgaacgtt accaaactga acgctgggtt
     1501 gactttgctg ctaacgccaa taaggattct ggtggttatg ccgctgatcc ctacaaggtt
     1561 catccgtatg tcttaatgag ttggactgga agaatgtctg atgtttatac ccttattcat
     1621 gaaatcggtc actctggtca attcattttt tcagataatc accaaagtta cttcaatact
     1681 catatgtcaa cttattacgt cgaagcgcca tcgaccttta atgaattgat gcttagtgat
     1741 tacttggaac atcagtttga tgaccctcgt caaaaacgtt ttgctcttgc tcaccgttta
     1801 acagatactt actttcataa cttcatcact cacctattgg aagcggcttt ccaacgtaag
     1861 gtttacaccc tgattgaaga aggcggtacc tttggagctg accaattaaa tgcaatgatg
     1921 aaagaggtct taacagactt ctggggagat gccgttgaca ttgacgatga cgcagctctt
     1981 acctggatgc gccaagcgca ttactacatg ggcttatata gttacaccta ttctgctggg
     2041 cttgttatgt caacagctgg ttaccttaac cttaaaaaca acccagatgg ggcaaaagaa
     2101 tggcttgatt tcctaaaatc aggtggcagc cgtacgcctc ttgatactgc aatgctcatt
     2161 ggggcagaca ttgccactga aaagcctctt cgagatacca ttcaattctt gagtgatacc
     2221 gttgaccaaa ttatcaatta caccgaggag atgtcacatg cctaatctct atgattttac
     2281 ggttaaggct caaaacggga atgacctttc cttagctgct tataaagaaa aggttgtttt
     2341 gattgttaat acagctacca agtgtggatt aaccccacaa taccaggcct tgcaagcact
     2401 atatgacaca taccatgaca agggatttga ggtcttagat tttccttgca accaattttt
     2461 aaatcaagct ccaggagatg ctgaggagat taatcacttt tgcagtctga cctatcacac
     2521 tactttccct cgctttgtta aaatcaaagt gaatggtaaa gacgctgatc ctttgtttgc
     2581 ctggttaaaa gaagaaaaaa gtggtcccct tgggaaacgc atcgaatgga atttcactaa
     2641 atttctcatt gaccaaaatg gtcaagtcat taaacgctat tcctcaaaga cagatcctaa
     2701 actaattgaa gaagacctta aagcacttct tggttaatac aaaatagact tggcgtgcca
     2761 agtctatttt gtattatagt tactatttaa aaacttcttg ataaaattca atgctatcac
     2821 catctttaac ggtgatttga tcggcaccct tatctgccat ctttccattc accttaaaaa
     2881 gccagtattt gttagctttc gtgtcttgct caataccatc aatagcagtg ataaagccat
     2941 ctttttcttt gacctcataa ttatctttga gaatctcaag caccgtatct ccttttccaa
     3001 aagaaacttt ttcatcaatg gtattggtat cttctttgac aactaagcga acgtgatggt
     3061 ctgcttttgg aacgcttgga gtctgtgttt gttttgttcc ttgcgagcaa gcaacaagta
     3121 gcatagcaac aagcgctaga aatcctgttt taaatctttt gttcaccgat taacctccta
     3181 aataacatca tcacaactgg aaaaaagacg agagttgata aagcatgcgc tatattaaag
     3241 ggcatcccag ctagaacata gctccaccaa ggcatactat acaaatacgc aaaacaagtg
     3301 tctatcaaca ccccataaag gaggccacat aaggcggcca agaaagcctg acagccaagc
     3361 tgatatttgg ggaattgctg agacagaggg tacaagacaa aacgccataa taaaagaaca
     3421 agcgcaaaac aagtaacttg ccaaaagacc caagggccaa atcccagcaa aaaggccgat
     3481 aaaaataagc acaacatcat aactaataca gcctcggcta acccaaagta aagaagataa
     3541 ctgagtaaaa atgccgtcac aggctgcacg ttaggcaggc tgctaaaaac cattctcaac
     3601 acaacacata gggctgacat gatagcaatg cgactcataa gcttagctga cataaacttc
     3661 ttcctcgttt ttttctctat tatgacagat ttacagagcg tttaccagtt tggagctcac
     3721 ttatttatca aatgataata acatgagaat taaataacaa aacaatactt gctgactgtc
     3781 atgatatagt caaagactaa actaatattg taaagaatat agctaccaac tataagagat
     3841 agtgacataa acttgacata gttagggagg atctcatttg taacttctaa aagataatac
     3901 cttaaaatgc aagacaatat aagaaaacag ttaggatatt ttgatttttt ttagtaatta
     3961 gggtacaata acagttaatg agaagaagaa taaaacctat tgttgtgcta gtcttcttta
     4021 ttttactggc aatggtattg attatcggaa aaagacaagc taatcatgcg aaacaaaaag
     4081 aagttgaaga cgctaagagt catattccta ttgcaaccag taatcctagt aaagcaaaaa
     4141 ctagcacttc tgaaaccgaa gactttattt taaatcctat tgttgatgtt tccggctggc
     4201 aactccctga agaaattgat tatgacacat tgtcgcgtca catctctggc gcaattgtac
     4261 gtgtctatgg aggatcgcaa attacagctc ataacaacgc tgcttttaca actggaattg
     4321 acaaatcttt taaaacgcat atcaaagaat ttcaaaaacg aaatgttcct gtagctgttt
     4381 acagttacgc acttggtcgt agtactaaag agatgaaaga agaggcccga actttctaca
     4441 aaaacgctgc tccctataat ccaacttact actggattga tgtcgaagaa gccactatga
     4501 aagatatgaa taaaggcgta acagccttcc gagaagagct taaaaaactt ggtgctgaaa
     4561 atgttggtct ctatattggc acttatttta tggcagaaca agatatttca acaaaaggtt
     4621 tcgatgctgt atggattccg acttatggta gtgattctgg ctattatgag gctgctccta
     4681 atacaacctt agattacgat ttacaccaat atacctcaca aggttatctc agtggtttta
     4741 acaatgcttt agatttaaat caaattgccg taacaaaaga cactaaaaaa acgtttgaga
     4801 agttatttgg caactccaac aattaagagg ttagcatcat ggctaatctt ttttgtagtt
     4861 tacatcttac caagaaaaac tctaggtcaa taatttaaac ccccttatcc ttgacaagga
     4921 gattatctca tcatataatg aaagcactat aatatttcac aaaggagtgc tttggctgag
     4981 atcgcaattg cgaaatcctg aggacctgat cttgttagta caagcgtagg gattgtgacc
     5041 aataatcaat gattggaaag cgatactctt tttgtgcaca aaaggagttt tttatgttac
     5101 caaacaccaa tgtcaaatac cttatcgaag ctgctatttt tgctgcattg gcgatgaccc
     5161 tttcatttat tccagatttt gctggctggt ttagtccttc ttatggtgct attgcactgg
     5221 ttattttctc tttgcgccgt gggttaaagt acggcatgtt ggcgggtctc atttgggggc
     5281 tacttcactt tgtacttgga aaagtttact atctcagcat gtcccaagtc tttattgaat
     5341 atatcttagc ctttacctct atgggattag ccggtagttt ctctgatagt ttgattaaaa
     5401 ctttaagaag acaacaaacc ttttttgccg tttttcttgc aataatggct agtttactag
     5461 ccgttacagt gcgttacttg tggcacttcc ttgcaggaat cattttctgg ggcagttacg
     5521 cccctaaagg catgtccgct gtctggtatt cttttagcgt taatggtaca gctggtgtcc
     5581 taactttttt aataacatgt ttagcgttaa tgattgcttt accaattcat cctcaattat
     5641 ttgatcccaa agactaattt ttagtcattt gaatcttaca ttaaaaaaaa atggtataat
     5701 aatgttgtta taccattttt ttatgtttag ataaaggtaa ggacttgata tgactaaaaa
     5761 aacaaaactc aaaaaaacct tagtagaaca aatcctcgat aaggctaata ttgctcatca
     5821 aggactcaag cttaatgctt tagaaggcga ctttccagat gatcttcaac catcagatat
     5881 ttacaagaca cttgctctga ctggcgatca aacaggtcct ctaattggca ttattccatt
     5941 aacagaacac ttatcagaaa aacaattggc aaaagtatca ggtaacaaaa aagtgtccat
     6001 ggttcctcaa aaagacttgc aaaagacaac aggctatatt cacggtgcca ataatcctgt
     6061 tgggattcgt caaaaacatt catatcctat ttttattgac caaactgcac tggaaaaagg
     6121 tcaaataatc gtttcagctg gtgaagttgg gcgttctata aagatttcta gccaggctct
     6181 agctgatttc gttggcgcaa gctttgctga tttaaagaag agaaaatgaa cactactgcc
     6241 aactttttct ggttactttt ttatttttag cgctcgcatc agttatctct aaaaggaggc
     6301 ttaaatgaaa ttatattttg taagacatgg gaaaaccctt tggaatttag aaggtcgctt
     6361 tcaaggcgct gggggcgatt cgccattact tgaagaggca aaagacgaaa ttcacttact
     6421 tggcaaagag ttagctaagg tggcttttga tgccgtttat actagcgacc ttcaaagagc
     6481 tatggcaaca gcagctatta tcttagatgc ttttgaccaa cagccaaaac tttaccacac
     6541 cgaccagctt cgggagtgga gattaggtaa attggaaggg gctaaaatag caacaatggc
     6601 agctatatat ccccaacaaa tgctggcttt tcgggagaat ctagcccagt tcaaaccaga
     6661 tcagttcgag gctgaatcaa tttaccaaac gactcaacgt gtctgccatc ttatccaatc
     6721 gtttaaagat aaacattatc aaaacgttct tattgtagga cacggcgcta acttaacagc
     6781 aactattcgc tcattattag gttttgaacc tgctctttta ctcgctaaag gtggcttaga
     6841 taatgccagt cttactattc ttgaaactaa agattattta acttatgact gtttaatttg
     6901 gaatgataaa tcttttctag aggtgtaaaa ccactagaca ataataaaaa aagctaacac
     6961 attgatctct tactgacaag acactcactt tgctagcttt ttttattatt atctagtggg
     7021 ctattatggt ttcatctatc tggcatattt taagcgcaaa actttttctt gttgcttggt
     7081 taaccgaagc aagataacca ccttgtcaat gctcatgttg ctttcaagta aacgctctgc
     7141 tgccatcatc aagccattat tgattcccat ctgtaaaatt ggatttttct tgtcattgac
     7201 atcaaaaata aatttattat caggtaaatc aaagagtact ttaacggcac caaagagctg
     7261 ttcaaagtta tcaatagcca catcataaac aggcttagtt cctggtttta gctgacgacc
     7321 tctatgatta aaccacatgg aatgccaacc accattaaag gcacccatga tatcattgtc
     7381 ataagagtcc ccaacatata gtgtggtttg tgggttcatg tcaaattgtt ctgctgccag
     7441 gttaaaaatt tctttttctg gtttttgaaa gcctgttgct tggctcacaa tgacacgctt
     7501 agggtcaata taatcataaa gccctaattt acgaactttt ttcagctgat gttctgttgg
     7561 cccattggta ataatcccca taggaacatt ctttgatttt aaaaagtcaa gggtcatgcg
     7621 catctcatct aacatggtaa tgttctcaag ttcgtgctca tagacttctt gaaaatgtac
     7681 tcccgcagct tcgtcgattt ctcgatagcc aaactcaagc agtgtttcct tgcaacgcca
     7741 gaatcggaaa tattctgtgg tccactcccc tgccattact cttgggaatc caacatctga
     7801 ataatgacga aagcgaatat aagcttgatt cattactgac atatcaaaat ctgggaagca
     7861 cttttccatg gcgattcgat aaggcgcttg ctggtcgtaa attgtgtcat ccacatcaaa
     7921 aacaatagca gtaatcatga gtctcttttc ttttctagtt taatatccca atcattatac
     7981 tattttccat taggcttttc aattttattt tcaaaaaagt gccccgctgc agtcgcattt
     8041 tccaacgaaa tataggtgta ttctagcctc ttttatgata caatggaaag gaaatcgtat
     8101 tggaggaaaa tatgtctaat caacacatcg aagaattaaa tgaccaacag attgtccgtc
     8161 gtgaaaagat gatggctctg gctgaacaag gaattgatcc tttcggtaaa cgctttgacc
     8221 gcacagccaa ttctgctgaa ttaaaagaaa agtatgctga taaaacaaaa gaagaattac
     8281 atgagttaaa cgaaacagct attgttgctg gccgcttgat gacaaaacgt ggcaaaggta
     8341 aggttggttt tgctcatctt caagaccgtg aaggccaaat ccaactttat gttcgcaagg
     8401 actctgttgg cgaagacaac tatgaaatct tcaaaaaagc cgatttaggc gactttatcg
     8461 gcgttgaagg cgaagtcatg cgtacagaca tgggcgagtt gtcaatcaag gcaaccaagt
     8521 tgactcacct ttctaaatca cttcgtcctc ttcctgaaaa attccacgga ttaactgata
     8581 ttgaaaccat ttaccgtaag cgtcaccttg atttaatttc aaatcgtgaa agctttgacc
     8641 gcttcgtgac ccgttccaaa atgatttcag aaatccgtcg ttatttagac ggtcttgatt
     8701 tcctagaagt agaaacgcct gtgcttcata acgaggctgg tggtgctgct gctcgtcctt
     8761 tcgttactca ccataatgct caaaacattg atatggtgct tcgtatcgca acagaacttc
     8821 atttgaaacg tttgattgtt ggtggtatgg aacgtgtcta tgaaattggt cgtatcttcc
     8881 gtaacgaggg aatggatgct actcataatc ctgagttcac ttctattgag gtttaccaag
     8941 cctatgcaga ctatcttgat atcatgaacc tgacagaagg cattatccaa catgctgcta
     9001 aagctgttaa aggtgatggc cctattgatt atcaaggaac cgaaatccgt atcaatgaac
     9061 ctttcaaacg tgtccacatg gtagatgcta tcaaggaagt gacaggtgtt gatttctggc
     9121 ctgaaatgac tgtcgaagaa gctattgcac ttgccaaaga aaaacaggtc ccactcgaaa
     9181 aacacttcac aagtgtcgga catatcatca atgctttctt cgaagaattc gttgaagaaa
     9241 ccttggtcca accaaccttt gtctttggtc atcctgttga agtatcacct ttggctaaga
     9301 agaatccaga agatacccgc ttcaccgacc gcttcgaact tttcatcatg accaaagaat
     9361 acgccaatgc tttcacagag ttgaatgatc ccattgacca attatctcgt tttgaagcac
     9421 aagcacaagc taaagaactt ggtgatgacg aagcaactgg cattgactac gacttcgttg
     9481 aagctcttga atatggtatg ccgccaaccg gtggactcgg aatcgggatt gatcgtcttt
     9541 gcatgttatt aacaaacaca acaacaatca gagatgtgct tttattccca acgatgaaac
     9601 cttaactttt ttaagatttg tattcatatc ttaaaaccga taagtggcaa tataacataa
     9661 cgttttatta tctctaacca gtcttcatta ccatgaagac tggtttatta acagtagctt
     9721 atagaaagtg acttaacaag atgaccaaag aatatatctc ctacattagt gctgctatct
     9781 tcttgcttta tggcctttta gctgccaaat ggatttttct tgggctaggc ttaattttgg
     9841 tacttgtcgg ccttgctgac cgcctcaatt caaaaaaata atccttctat tcgcccctgt
     9901 cctcttaacc tagcgatgtc atcaccaggt taagaggaca agggcgaacg agggattatg
     9961 tttcgattaa ggtagcatta cttacgaaaa gcgtctaaga tggttttaaa ttcttcatta
    10021 gtaagagtaa tgcctttccc cattttcgta tggtctggac tccacgtgcg gatatcccat
    10081 ttcgcttctg cgccattaaa gctgacgcgg ttcaattctt tcgtccaacc tttatcattt
    10141 tctgacaagg tgagtaagtg ttcttcaatt ttaaacgtaa attctgccat tatt
//
LOCUS       DAUELZ010000058        10158 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.059, whole genome shotgun sequence.
ACCESSION   DAUELZ010000058 DAUELZ010000000
VERSION     DAUELZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.059"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(38..658)
                     /locus_tag="VHI09_001136"
     CDS             complement(38..658)
                     /locus_tag="VHI09_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEQ6121018.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSENITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPNQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
     gene            complement(960..1307)
                     /gene="ssrA"
                     /locus_tag="VHI09_001137"
     tmRNA           complement(960..1307)
                     /gene="ssrA"
                     /locus_tag="VHI09_001137"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1399..2013
                     /locus_tag="VHI09_001138"
     CDS             1399..2013
                     /locus_tag="VHI09_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEQ6121019.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIVLFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            complement(2140..2925)
                     /locus_tag="VHI09_001139"
     CDS             complement(2140..2925)
                     /locus_tag="VHI09_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121020.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNVNELLEGFFNSFKMDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHNHIFNIN
                     TSADSFPFTEQKTTYLLILEQFIEMIMFYLATNFIIKNKLNLQ"
     gene            complement(2935..3633)
                     /locus_tag="VHI09_001140"
     CDS             complement(2935..3633)
                     /locus_tag="VHI09_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121021.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            complement(3633..4004)
                     /locus_tag="VHI09_001141"
     CDS             complement(3633..4004)
                     /locus_tag="VHI09_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ6121022.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFNHTEIIPVLTSFLKEDSQ"
     gene            4189..7299
                     /locus_tag="VHI09_001142"
     CDS             4189..7299
                     /locus_tag="VHI09_001142"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEQ6121023.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELADCQQLTTFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQDLTEAGLKEKGLWKEPYQSRLLHELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSDYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIIEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKILDNLDGLLVFVNELGSLFSDSSFSWVDAKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            7379..8392
                     /gene="pfkA"
                     /locus_tag="VHI09_001143"
     CDS             7379..8392
                     /gene="pfkA"
                     /locus_tag="VHI09_001143"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEQ6121024.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            8455..9957
                     /gene="pyk"
                     /locus_tag="VHI09_001144"
     CDS             8455..9957
                     /gene="pyk"
                     /locus_tag="VHI09_001144"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEQ6121025.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICRETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
BASE COUNT         3190 a         1683 c         2049 g         3236 t
ORIGIN      
        1 gaaatcaatc tttttagcaa aatatattac gcatttttta ttcttcaagt cctacctgag
       61 acatttctgt taactgatta ttttcaaaaa cgagagaaat attagctccg cttgtatctg
      121 tctttaagcc actaacccaa atcgcttgca aagtttgatg atcactagat gacgcttggc
      181 tataattatc aggttctgtt aagatctttt taacgtcttc ataagacatt cctttttgta
      241 attgttggta ttctttttgc gataagccta actctcttgc aaaggtaaaa ttagagatgg
      301 ttttaacaat actactattt tgataaagat taacagtgag agtaacttga tcaaactgcc
      361 aagtataagc gtcgattgtt acttcgcctg ctgtttttgg atcatgttgg ttaggctctc
      421 caaagagtga aataagttct tcaaggcttg ttcctcctgt aaatgaggaa tcaacagtcg
      481 caagtttaat tttttcaaag ttagcgcgtt tatcttgatg agcggcttgg tgtagtcggg
      541 gagtaatgtt ttctgaatca gacggttgag gctctttatc tttcgttgtt gaacaagcta
      601 ccaaaaagct agtaaaaaaa gagatgagaa gaagtgttag taagtgtttt tttgtcatat
      661 gttatttcct aaactatttt tgctgtatta tatcataaat tataaacaag atatagctat
      721 gattccccat aacgcatgcg ctatggggaa tttagtacta ttttgattct tttttttaga
      781 tattcatgaa aaaaatggtc aaaaagctct gatatgacaa ggttttgagt ggtattgatg
      841 gagtatcggt aagcgtgatg gtatggctat agggataacg gaaactcatg aacaagacaa
      901 aaagaaaaaa ccttgatgta acaaggtttt agtaagttat gattacttac ggtaagcatt
      961 gatggagctg gtgggagtcg aacccacgtc caaacacctg ccaacatatt tgtctacgtc
     1021 cataggttat gtcttcattt aacaaactaa agacacataa ctcaagccat tagtttgcga
     1081 gtctatcaat ctcctgtcac ggaactagac aatttcgtaa ccgtagcttg ctattttgag
     1141 acttctaaca ggacacaagc aatcttgtag aagtcacgct agctggtttt taggcagcta
     1201 aagcgtaaga gtttgtattt tttgcagtta tatttaactg gcaatttaca tctgccgatg
     1261 ggacgcaaaa catgcctcat aatgcctgtc gaatccgtaa caaccccaaa tagataatta
     1321 accaatttct tacactaact attctatcaa aacttttttt aaatagcaaa attttacagc
     1381 ttatggtatg attagtctat gacaaaacgt aattcaaaag cctatatatt atggcaaaaa
     1441 attatcaaaa ttttaggaat tattgcatta attggtactt ttttcctcgc tttttggctg
     1501 tatagactag gtatattaaa cgatagtaat gctcttaaag acttagtgca gcgctataga
     1561 ttatgggggc cgtttgtttt tattgtcgtg cagatcatac agattgtttt tcctgtgatt
     1621 cctggaggcc ttacgacagt tgcaggtttt ttaatctttg gccctgtaac aggttttatt
     1681 tataattatg taggcatcat tattggcagt atagtcctct tcttgcttgt taagacttat
     1741 ggtcgtaaat ttatcttact ctttgtaaat gacaaaacat tttacaagta cgaaaggcgt
     1801 ttggagacac caggatatga aaaattattt attttttgta tggcatcgcc tgtttctcca
     1861 gctgatatta tggttatgat tacgggattg acggacatgt ctttaaagcg ctttgtaact
     1921 attcttttaa taactaaacc aatttctatt atcggctaca gttatttatt tatttttgga
     1981 aaagatgtta tatcttggtt cttacagcat taaggtttgg tgttaatttg ataaaccgct
     2041 tctatttgat ggaagacggt tttttaattt actatgaaca ctgaaaaaaa ctttctttta
     2101 aactatagtg tgtcatagtt taaaagaaag tcttaacgct tactgtaggt tgagtttatt
     2161 tttgataata aaattagtgg cgaggtaaaa cattatcatt tcaataaatt gttccaaaat
     2221 aagaagataa gttgtttttt gctcagtaaa tgggaaacta tcggcacttg tattaatatt
     2281 aaagatgtga ttgtgaacat atgttgcagc aacgctaatc agaataacta atataaaata
     2341 tgcaataaag gccatcaagc ctcgccgatt agaaaaaagt tgacctatag aaatggaaag
     2401 ataaattaat aagatactcg taaatgttga taataggaca taggctagta cagttagcat
     2461 attgatgaaa taatccatct taaaactatt aaagaatcct tctaatagtt cgttgacgtt
     2521 aaacattggt acaattacaa tagcgatagc aaatgcaagg atgatagtat tgaaaacact
     2581 gcaaataaaa gaagctagta gttttgataa aataatttga tgcgaattga cgggtaaagt
     2641 caacgttaag tatccttccc gtccaaaaac acttttgcta aaacgtttga taatgataag
     2701 taatgttgat aagagggagc cagcgatcag ggagccaaaa gttaaaccta atgtcagagg
     2761 caacatctta ttggttagta ctccaaatag tccatcacta ttgctttgag caaacagttt
     2821 tatcgtaaat gacaaaatgg cagcgatggc aataacgaaa gcattaagcg caaagtacca
     2881 ttttccaata gatctaaatt cgtattttaa taattttcca aacatgatat cctcctaaac
     2941 cttataagtt tccctaaaga gggaatcaat agattgctgg tattcttgtc ttagatcatc
     3001 agcattgcca gataagaaaa gtcttccttg tttcaagaaa atgacttcgt caaggatagg
     3061 ttcaatgtcc gaaatcaagt gggttgagat aataacagaa gcattttcac aataactatt
     3121 aatgatggtt tttaggatgt agtctcttgc ggcagggtca actccaccaa taggttcatc
     3181 aagtacatag agtctggctt tacgactcat cacaagaatt aattgtactt tttctttgtt
     3241 tcctttggat agagttttga agcggtcttc agggtctagt tctaaatctc ttagaaggct
     3301 tgttgctttg gatttgtcaa agtcgctgta aaaatcacta aagaactcca acatgtcctt
     3361 aattctcata ttttcattga gataagtagt atctggtaaa taagaaataa ttttttttgt
     3421 ctcaacacag ggacggtagc catcaatgac aatttctcct ttatttggtt gtaaaaggcc
     3481 attaatgagt ttaattaaag ttgttttacc acttccattt ggacctaata atccgataat
     3541 tttaccgttc gggatagtaa tggttaagtc atcaatagct tttttttcac ggtaagattt
     3601 tgaaacatga tgtagttgca ataggtgtgc cattattgtg aatcctcctt taaaaagctc
     3661 gtcaaaacag ggataatctc agtgtgatta aatcccattt ttgtcatatt ggtgataaat
     3721 gattctaatt cactaattgc taactctcgt cttttacgag caattaattt ttgatcgtca
     3781 gttacaaaac ggccagcagt acgttgcgaa taaaccattc cttcccgttc aagttctgta
     3841 aacgctcgtt gcatggtgtt aggattgaca cctgcgattt ctgcatattc acgaacagtt
     3901 ggaagttgat caccactctt gatttcttgg ctaataattt gcatcatgac atgttgtgct
     3961 atctgcgcat aaataggaga tttttcatca aatttccaag acatgtggtc tcctttctga
     4021 gtaatataga tatttgtact aattaaatgg tacagaaaaa aaaggggaat gtcaagtttt
     4081 atgccatttt tcactctttg agaagtagac gtagattaga gagaaaattg aagcaagaaa
     4141 acctttttca tagtataata gggaacaaga taaccaaagg aggaacttat gtttgctcaa
     4201 cttgatacta aaactgtata ctcatttatg gatagtttaa ttgacttaaa tcattatttt
     4261 gaacgagcaa agcaatttgg ttaccacacc ataggaatca tggataagga taatctttat
     4321 ggtgcttacc attttattaa aggttgtcaa aaaaatggac tgcagccagt tttaggtttg
     4381 gaagtagaga ttctctatca agagcggcag gtgctcctta acttaatcgc ccagaataca
     4441 caaggctatc atcagctttt aaaaatttcc acggcaaaaa tgtctggcaa gcttcatatg
     4501 gattacctct gccaacattt ggaagggata gcggttatta ttcctagtaa aggttggagc
     4561 gatacattag tggtcccttt tgactactat atcggtgttg atcagtatac tgatttatct
     4621 catatggatt ctaagaggca gcttataccc ctaaggacag ttcgttattt tgcgcaagat
     4681 gatatggaaa ccctgcacat gttgcatgcc attcgagata acctcagtct ggcagagacc
     4741 cctgtggtag aaagtgatca agagttagca gattgtcaac aactaaccac cttctatcaa
     4801 acacactgcc ctcaagctct acagaattta gaagacttag tgtcaggaat ctattatgat
     4861 ttcgatacaa atttaaaatt gcctcatttt aatagagata agtctgccaa gcaagaattg
     4921 caagacttga ctgaggctgg tttgaaggaa aaaggattgt ggaaagagcc ttatcaatcg
     4981 cgcttactgc atgaattggt cattatttct gacatgggct ttgatgatta ttttttgatt
     5041 gtgtgggatt tacttcgctt tggacgcagt aaaggctatt atatgggtat gggacgtggc
     5101 tcggcggcag gtagtctagt ggcttatgct ctgaacatta cagggattga tccagttcaa
     5161 catgatttgc tatttgagcg ctttttaaac aaagaacgtt atagcatgcc tgatattgat
     5221 atcgatcttc cagatattta ccgttcagaa tttctacggt atgtccgaaa tcgttatggt
     5281 agcgaccatt cggcgcaaat tgtgaccttt tcaacctttg gacctaaaca ggctattcgt
     5341 gatgttttca aacgattcgg ggttccagaa tacgaactga ccaatctcac taaaaaaatt
     5401 ggttttaaag atagcttggc tactgtctat gaaaagtcaa tctcttttag gcaggttatt
     5461 aatagtagaa ctgaatttca aaaggccttt gccattgcca agcgtatcga aggaaatcca
     5521 agacaaacgt ccattcatgc agctggtatt gtgatgagtg atgatacctt gaccaatcat
     5581 attcctctaa aatcgggcga tgacatgatg atcacccagt atgatgctca tgcggtcgaa
     5641 gctaatggcc tgttaaaaat ggattttttg gggttaagaa atttgacctt tgttcaaaaa
     5701 atgcaagaga aggttgctaa agactacggg tgtcagattg atattgcagc cattgattta
     5761 gaagacccgc aaacgttggc actttttgct aaaggggata ccaagggaat tttccaattt
     5821 gaacaaaatg gtgctattaa tcttttaaaa cggattaagc cacaacgttt tgaagaaatt
     5881 gttgccacta ccagtctaaa tagaccaggg gcaagtgact ataccactaa tttcattaaa
     5941 cgaagagaag gacaagaaaa aattgatttg attgatcctg tgattgctcc cattttagag
     6001 ccaacttacg gtattatgct ttatcaagaa caagttatgc agattgcaca agtttatgct
     6061 ggttttacgt taggcaaggc cgacttgtta aggcgtgcca tgtctaaaaa aaatctacaa
     6121 gaaatgcaaa aaatggaaga agactttatt gcttctgcta agcacctagg gagagctgaa
     6181 gaaacagcta gaggactttt taaacggatg gaaaaatttg caggttatgg ttttaaccgc
     6241 agccatgcct ttgcctattc agctttagct tttcaattgg cttatttcaa agcccattac
     6301 ccggctgttt tttacgatat catgatgaat tattctagca gtgactatat cacagatgct
     6361 ctagaatcag attttcaagt agcgcaagtt accattaata gtattcctta cactgataaa
     6421 attgaagcta gcaagattta catggggctg aaaaatatta aggggttgcc aagggatttt
     6481 gcttattgga ttattgagca aagaccattt aatagtgtag aggattttct cactagaact
     6541 ccagaaaaat atcaaaaaaa ggttttcctt gagcctctga taaaaatagg tctgtttgat
     6601 tgctttgagc ctaaccgtaa aaaaattctg gacaatttgg atggtttact ggtatttgtt
     6661 aatgagcttg gttctctttt ttcagattct tcctttagtt gggtagatgc gaaagattac
     6721 tcagcaactg aaaaatattc tttggaacag gagatcgttg gggttggcat gagcaagcat
     6781 cctttaattg atattgctga gaaaagtacc caaactttta ctcccatttc acagttagtc
     6841 aaagaaagcg aagcagtcgt actgattcaa atagatagca ttaggatcat tagaaccaaa
     6901 acaagtgggc agcaaatggc ttttttaagt gtgaatgaca ctaagaaaaa gctcgatgtc
     6961 acactttttc cacaagagta tgctatttat aaagaccaat taaaagaagg agaattctat
     7021 tacttaaaag gtagaataaa agaaagagac catcgactgc agatggtgtg tcagcaagtg
     7081 caaatggcta ttagtcaaaa atattggtta ttagttgaaa accatcagtt tgattcccaa
     7141 atttctgaga ttttaggtgc ctttccagga acgacaccag ttgttattca ctatcaaaaa
     7201 aataaggaaa caattgcatt aactaagatt caggttcatg taacagagaa tttaaaggaa
     7261 aaacttcgtc cttttgttct gaaaacggtt tttcgataaa aatggacaat tagtgaagta
     7321 ttttgcaaat gaatgtgcta taataagacc gttaaattaa ttaaaggagt accagaaaat
     7381 gaaacgtatt gctgttttaa ccagtggcgg cgacgctcct ggtatgaatg ctgctattcg
     7441 tgcagtcgtt cgtaaagcaa tttcagaagg aatggaagtt tatggtatca accgtggcta
     7501 tgcaggtatg gttgatggcg acattttccc actgggttca aaagaagttg gtgataaaat
     7561 ctcacgtggt ggaactttct tatactcagc tcgttatcca gaatttgctc aacttgaagg
     7621 tcaattggct ggtattgagc aacttaaaaa acatggtatt gagggtgttg ttgttattgg
     7681 tggggatggt tcttatcatg gcgctatgcg cttgacagag cacggcttcc cagcggtagg
     7741 tatcccagga acaatcgata acgatattgc gggaactgac tacactatcg gatttgatac
     7801 agcagttaac acagcagttg aagctattga taaacttcgt gacacctcat caagtcacgg
     7861 acgtactttt gtggttgaag tgatgggtcg caatgcgggt gatattgctc tttgggcagg
     7921 tattgctagt ggtgctgatc aaatcattgt tccagaagaa gagtttgata ttgaaaaagt
     7981 ggcatcaaca atccaatatg attttgaaca caaaggtaaa aatcaccaca ttatcgtctt
     8041 agctgaaggt gttatgagcg gtgaagcttt tgctcaaaaa ctgaaagaag ctggcgataa
     8101 gagtgacctt cgtgtgacaa atcttggtca cattcttcgt gggggttcac caactgctcg
     8161 tgaccgtgtc attgcttctt ggatgggttc acatgctgtt gagctactta aagaaggcaa
     8221 aggtggtttg gctgttggta ttcacaatga agaacttgtt gaaagcccaa tccttggtac
     8281 tgcagaagaa ggtgctttgt tcagcttgac tgaagaaggc aagattattg ttaataatcc
     8341 acacaaagct cgtttagact ttgcagcttt gaaccgttca ttgtcacaat aagtttagtt
     8401 aaaattgtta aatcgtcaat tattgacaaa aaaataaggg agtttcagag taaaatgaat
     8461 aaacgcgtaa aaatcgttgc aacacttggt cctgcggttg aaatccgtgg tggtaaaaaa
     8521 tacggagaag atggctactg ggctggtcaa cttgatgttg aagaatcagc aaaaaaaatc
     8581 gctgaattaa ttgaagctgg tgcgaatgtt ttccgtttca atttctcaca tggtgaccac
     8641 aaagaacaag gtgaccgtat ggcaactgtt cgccttgcag aagaaattgc tcgtcaaaaa
     8701 gttggtttcc ttttggatac taaaggtcct gaaatgcgta cggaattgtt tgcagatgat
     8761 gctaaagaat tcagctatgt cactggagaa aaaatccgtg tagcgactac acaaggtatt
     8821 caatcaactc gcgatgttat tgctttgaac gttgctggta gccttgacat ctacgatgaa
     8881 gtagaagtag gtcatactat tcttatcgat gatggtaaac ttggtttgaa agtgatcgac
     8941 aaagatatcg caacacgtca atttatcgtt gaagtagaaa acgacggtat tattgctaaa
     9001 caaaaaggtg ttaatatccc taacactaaa attcctttcc cagcacttgc ggaacgcgac
     9061 aatgctgata tccgttttgg tcttgaacaa ggacttaact ttattgcaat ctcatttgtt
     9121 cgtacagcca aagacgttga ggaagttcgt gaaatctgtc gcgaaactgg taacgaccat
     9181 gtgcaattat ttgctaagat tgaaaaccaa caaggtatcg ataatcttga tgaaatcatc
     9241 gaagctgctg atggtatcat gattgcacgt ggcgacatgg gtatcgaagt accatttgaa
     9301 atggttccag ttttccaaaa aatgattatc actaaagtga atgcagctgg taaagcagtt
     9361 atcacagcaa caaacatgct tgaaacaatg actgaaaaac cacgtgcgac acgttctgaa
     9421 gtatctgacg tcttcaatgc tgttatcgat ggtactgatg caacaatgct ttcaggagag
     9481 tcagctaacg gtaaataccc ggttgaatct gttcgtacaa tggcgactat tgatcgtaat
     9541 gcacaaactc ttcttaacga atatggtcgt ttagattcat ctgccttccc acgtaccaat
     9601 aaaacagatg ttattgcttc agctgttaaa gatgccactc attcaatgga catcaaactt
     9661 gttgtgacta ttactgagac aggtaataca gctcgtgcga tttctaaatt ccgtccagat
     9721 gcggatatct tagctgtaac atttgatgaa aaagtacaac gtgctttgat gattaactgg
     9781 ggtgttattc ctgttcttgc tgaaaaacct gcatctactg atgacatgtt tgaagtagca
     9841 gagcgtgttg ctgtggaggc aggcttagtt caatctgggg ataatattgt tattgttgct
     9901 ggtgttccag ttggtactgg tggaacgaat actatgcgtg ttcgtacagt aaaataaatg
     9961 tgttataact atctacctaa aggttagaag gcaagcttct aacctttttt gatatcaaaa
    10021 atatcatgtt aattcactag tgttaattta ctttaatgag tctaagtttg tattttatac
    10081 agaatgagta acgtttattg gtattgatca aaaattgttt actttttttc tagcttagaa
    10141 catggtataa taagacaa
//
LOCUS       DAUELZ010000059        10109 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.060, whole genome shotgun sequence.
ACCESSION   DAUELZ010000059 DAUELZ010000000
VERSION     DAUELZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.060"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..332)
                     /gene="cadX"
                     /locus_tag="VHI09_001145"
     CDS             complement(<1..332)
                     /gene="cadX"
                     /locus_tag="VHI09_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEQ6121026.1"
                     /translation="MKKDSICQVNVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLTVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQL"
     gene            complement(344..958)
                     /locus_tag="VHI09_001146"
     CDS             complement(344..958)
                     /locus_tag="VHI09_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEQ6121027.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGEILEKYSRWFIAVVYLGLGIYILIENNSFDMLWAVLG"
     gene            complement(1252..1401)
                     /gene="rpmG"
                     /locus_tag="VHI09_001147"
     CDS             complement(1252..1401)
                     /gene="rpmG"
                     /locus_tag="VHI09_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ6121028.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            complement(1417..1599)
                     /gene="rpmF"
                     /locus_tag="VHI09_001148"
     CDS             complement(1417..1599)
                     /gene="rpmF"
                     /locus_tag="VHI09_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEQ6121029.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            1819..3099
                     /locus_tag="VHI09_001149"
     CDS             1819..3099
                     /locus_tag="VHI09_001149"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEQ6121030.1"
                     /translation="MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVQDMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDIYLA
                     VLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFENISEQLLS"
     gene            3437..5185
                     /gene="aspS"
                     /locus_tag="VHI09_001150"
     CDS             3437..5185
                     /gene="aspS"
                     /locus_tag="VHI09_001150"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEQ6121031.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DIAMNSYGSDKPDTRFEMLLQDLTVTVKGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLIAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKGNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            5178..6119
                     /locus_tag="VHI09_001151"
     CDS             5178..6119
                     /locus_tag="VHI09_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ6121032.1"
                     /translation="MIKKTTYRKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIMPQVTLTTDPLINAIFGGLVMGLGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            6139..7017
                     /locus_tag="VHI09_001152"
     CDS             6139..7017
                     /locus_tag="VHI09_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ6121033.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVMGILSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGLVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     DELGRGVTYLTGEGAYSGRRKKVIYVALGRRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            7060..7932
                     /locus_tag="VHI09_001153"
     CDS             7060..7932
                     /locus_tag="VHI09_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ6121034.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILNAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(7959..8267)
                     /locus_tag="VHI09_001154"
     CDS             complement(7959..8267)
                     /locus_tag="VHI09_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ6121035.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKACHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(8355..10046)
                     /locus_tag="VHI09_001155"
     CDS             complement(8355..10046)
                     /locus_tag="VHI09_001155"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEQ6121036.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKAKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLAHIFAKMGYQPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAETDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLELLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLQEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHAVGVGAIKFYDLKTDRMNGYDFDLETMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
BASE COUNT         2944 a         1771 c         2146 g         3248 t
ORIGIN      
        1 agttgattaa aaaaatctct gatttcctca tcttttataa aataatatat aattttcccc
       61 tctcttctag tgtccaagat gttttgattg gctagtttac gaagatggtg ggaggcagat
      121 gccatactga gatttagtaa acaggctata tcgcagacac agagttcttc aacagtaagg
      181 agataaaaga tgatatttat ctgtttatta tcggtaaact ttgataaaat acgaagtgat
      241 ttttggactt tttccttttc aaggtagttc gttgcggttg taacattttg ttgatttata
      301 acattcactt ggcagatact atcttttttc ataatatttt ctcctagcct aacacagccc
      361 atagcatgtc aaaactgttg ttttcaatca ggatatatat ccccaatcct aaataaacaa
      421 cggcaataaa ccatctgcta tatttttcca aaatttctcc aacagaaggg acttgtgcca
      481 atttttgggc agaaaaaacc aagagataaa tcatgactag aaaggtaagt aaagccacta
      541 tcaaattcgc taaatttaag gtagtaaaat acgggacaaa gacaccaata ttgtcagcac
      601 cacaacttgc aaaagtaatc atagcgacta gaaaaatcag gtttttatta tctttgcgca
      661 aaccttcttt ggcaatagct tctccatcag aatctcctaa aagcaaaact ttgaggccta
      721 ggaaaattgg aatcaaaccg agcaaaccta aaatctcttt actaggaata taatctaaga
      781 caaatgcaaa aagcaaactt agcaatatta gactaacaga gcctagaaat tgtcctaaat
      841 agatgttaat gatgtctttt ctgctttttc ttttggcaaa aaataacatt aggataataa
      901 gtaagtctac ggctgtccca gaatacagga ttattgaagt aacaacattt tgaatcataa
      961 aacacctcat tcaaatatat ttttgaatga attttaacat taaactttgt agatgtcaat
     1021 ttcagctcca ccaaaatata gataagaagt tagtgtacca aatataaaaa agccctgcca
     1081 tccaaatgat aacagggctt aacttcaata tccaaatgat atattcatct aaaaaaggta
     1141 gagtaaaagg tagagttctt attgatttat attacaatgt aatgaacttc aaaaaattca
     1201 aaaatcgctt aaaatcaatg ttttgacgtc taatgacgtg tagtgaaccc cttacttaac
     1261 ttctgtaaat gtaacgtgtt tacgtaattt tggtgagtat tttttcaatt gaagacggtc
     1321 tggagtgtta cgtttgtttt ttgaagtaag gtacaagcgt tcaccagatt ctttatgttc
     1381 aagtgtaata tttacgcgca tggtatctcc cttctattat ttagcttcgt tagctttagc
     1441 gattttacgt cctttgtagt atcctttaag ggatacacgg tgagaacgtg agtaatctcc
     1501 agtagtttcg tcgaattgta cagatggagc tgtcaatttg tagtgtgtac gacgtttgtt
     1561 tttcttcgct tttgacgtgt gacgtgcagg tactgccatt ttcttgttct tcctttcgat
     1621 gtgtaagttt tgattttcat caacttcact atgataacat atcttttttg taaagtaaag
     1681 ttacttgaca aaacttttta cttttacaat ttcatggtga aaatactatc aaactaaagc
     1741 aaatgatata tgcttgttta aaatataaga aaaaatggta gaatgtttaa agagaaaact
     1801 aatcaaagag gtaaacttat gaaacttcaa aaaccaaaag ggactcagga tattctgcct
     1861 ggggatgctg cgaaatggca gtatgtggaa tctgttgcgc gtgatacgtt tagccaatac
     1921 aattatggag aaattcgtac tcctatgttt gaacactacg aggtaatctc gcggtctgta
     1981 ggtgatacga ctgatattgt gacgaaggaa atgtatgatt tctatgataa aggagatcgt
     2041 cacattacct tgcgaccaga gggtacagct ccagtggtgc gttcttatgt tgaaaataaa
     2101 ttgtttgcgc cagaggtcca aaaacctgtc aaactttatt atatcggttc catgttccgc
     2161 tacgaacgtc ctcaagctgg tcgtttacgt gaatttcacc agattggggt agaatgtttc
     2221 ggcgctgcta atccagcaac agatgttgaa accattgcga tggcctatca cctctttgaa
     2281 aaattgggca ttaaggatgt cacccttcat ttaaatagtt tggggagtcc tgagagtcgt
     2341 gcagcttatc gtcaagcctt gattgactac ttaacaccaa tgcgtgatca attatctaaa
     2401 gatagtcaac gtcgcttgga tgaaaaccca ctgcgcgtgc ttgattctaa agaaaaagaa
     2461 gataaactag ccgttgaaaa agcgccttct attttggatt acttagatga ggaaagtcag
     2521 gcgcactttg aggcggtcca agatatgctt gaagcacttg acattcctta tgtgattgac
     2581 accaatatgg tgcgtggcct tgattactat aaccacacta tttttgaatt catcacgtct
     2641 gtggaaggtt ctgacttaac tatctgtgcg ggtggacgtt atgatagttt ggtaggttat
     2701 tttggtgggc ctgagactcc aggatttggc tttggtcttg gtttagaacg tctcttgatg
     2761 attattgaaa agcagggcat taccttgcca attgagacag agatggatat ttaccttgct
     2821 gtgttaggtg atggtgctaa tagcaaagct ttagaattgg ttcaagccat tcgccgtcaa
     2881 gggtttacag ctgaacgaga ttaccttggc cgtaagatta aggctcagtt taaatcagct
     2941 gataccttta aagcaaaatt ggtaatgact ttaggagaaa gtgaagttga agcaggcaaa
     3001 gctgtgatta agaataatcg tagccgtcaa gaagtggaag tcagttttga agatatgatg
     3061 accaactttg aaaacatctc agagcaatta ttgtcttaat gattttattg tcgtcataag
     3121 cttgtgttga tatgattgtt tagaagataa tcactaaaac aggtgtcact tctcttttta
     3181 gaagggagtt gcacctgttt gttttctgta aggggattga ttagctttct tagtattctg
     3241 tgaggacacc tgatatgtca aaatgctgac gactcagtat tcttgcttta tctttagaaa
     3301 aactcttttt aaatgggtgg ggtttttaag ggctttttgc tataatagta gggaatgaat
     3361 taattgattt atatagtgaa aggaattgga aacgagcagg tttcacagga ttctgtttgt
     3421 tttagggaat ttatttatga aacgtagcat gtatgcaggg cgtgttcgcg aagaacacat
     3481 tggaacgaca atcacattga aaggatgggt cagtcgtcgt cgtgacttgg gtggtctcat
     3541 ctttattgat ttacgtgacc gtgagggtgt gatgcaatta gtcattaacc ctgaggaagt
     3601 ttccagtgat gtgatggcaa ctgctgaacg cctacgtagt gaatatgtga ttgaagtgga
     3661 aggttttgtt gaagctcgac aacaagccaa tgataagttg gcgacaggaa tggttgagtt
     3721 aaaagtatct gctttgacca ttttaaacac agctaaaacg acgccttttg aaattaaaga
     3781 tgacgttgaa gtaagtgatg acacgcgttt acgttaccgt tacttagatt tacgacgtcc
     3841 agaaatgtta gaaaacttca agctacgtgc aaaagtgact cattctatcc gtaattacct
     3901 tgatgacctt gagtttattg atgtcgagac acctatgttg acaaaatcaa ctccagaagg
     3961 tgcgcgtgac tatctggtac caagtcgtgt tagccaaggg catttctatg ctttgccaca
     4021 aagtccgcaa attaccaaac agttattgat gaatgctggc tttgatcgct attatcaaat
     4081 tgtgaaatgt ttccgcgatg aagatctacg tggtgaccgt caacctgagt ttacacaagt
     4141 tgacttggaa acgtcattcc tttctgaaca agagattcaa gacatcgttg aaggcatgat
     4201 tgctaaagtg atgaaagaga caaaagaaat agatgtaacc ctaccattcc cacgtatgag
     4261 ctacgatata gcaatgaata gctacggctc tgataaacca gacacacgtt ttgagatgct
     4321 tttacaagac ttgacagtaa ctgtcaaggg aattgacttc aaggttttct cagaagcacc
     4381 agctgtcaag gcgattgtgg tgaagggaaa tgcagatcga tactctcgta aagatattga
     4441 caaattaact gagtttgcca aacaatttgg tgctaaaggc cttgcctggg ttaaggtgac
     4501 agatggtcaa ttggcaggcc cagtagccaa gttcttgata gctattgaga cagagttgtc
     4561 aagccaatta aaacttgctg agaatgattt ggttttattt gtagcggata ctcttgaggt
     4621 tgctaataat acattaggtg cccttcgtaa tcgtattgcc aaggaccttg acatgattga
     4681 tcagtcgcag tttaatttcc tttgggtggt tgattggcca atgtttgagt ggtcagaaga
     4741 agaaggacgt tatatgtcag ctcatcaccc cttcactctt ccaacgccag aatctgctca
     4801 tgagttagag ggtgatttgg ctaaggtacg ggcaattgct tatgacatcg ttttaaatgg
     4861 ttacgagctt ggtggtggta gcctgcgtat caatcaaaaa gagatgcaag agcgcatgtt
     4921 caaggctctt ggctttaccg ctgatgaagc taacgaccaa tttggtttct tactagaagc
     4981 gatggactac ggctttcctc ctcatggtgg cttggcaatt ggtcttgacc gttttgtcat
     5041 gttgcttgct ggaaaaggca atattcgaga agttattgcc ttccctaaaa ataacaaagc
     5101 ctctgatcca atgacgcaag cacctagtct tgtttctgaa aaccaattag aagaactcag
     5161 tcttcaaata gaaagtcatg attaagaaaa caacctatag gaaaaaagtt aaatatgtca
     5221 ttagtagagg ggccaagaaa gttggcctac tccacgctct aagaagtatt tcaagagaaa
     5281 aatatgcaga gaagatttcg gcttctctgc tttatggcat tctctctagt attgctgtga
     5341 attttttctt ccagcctggg catgtttatt caagtggagc aactggtcta gcacaggttt
     5401 tttcagctct tagtcatcgt cttttaggct atgattttcc catcgccttt gcgttttatt
     5461 tgattaatat tcctttgctt attttagctt ggtataaaat tgggcatcaa tttaccattt
     5521 ttacctttat cacagtcagc atgagttctt tctttattca aatcatgcct caagtgacgc
     5581 tgacgactga tcctcttatc aatgctattt ttggtggttt ggttatggga ctgggaattg
     5641 gtacaggtct caaatcacgt atctctagtg gggggactga tattgtcagt ttgaccctta
     5701 gaaaacgaac aggcaaggat gtgggcagtc tctcattgat ggttaatggt gcaattttag
     5761 cctttgcagg gattttattt ggctggcagt acgcccttta ttctatggtc tctatctttg
     5821 tatcaagtcg tgttacggat gccattttca ccaagcaaaa gaaaatgcag gcaactattg
     5881 ttaccagcca tccagagcgt gtgattcata tgatccataa acgtctgcat cgcggggtga
     5941 ccagtatcaa cgacgcagaa gggacttaca agcatgaaca aaaagcagtt ttgattacca
     6001 ttttgacatg tgaagaatat ccagaattca aatggctgat gttaaaaaca gacccacaag
     6061 cctttgtttc agtggctgag aatgttagaa ttatcggtcg ttttgtggaa gatgattaag
     6121 aaaaacacag gaggtgtcat gtcaattgac caacgtttac agttaaagaa attttgcttt
     6181 attattttag gagctgctat ctacgccttt gcttttgttt atttttacat ggctaatcgc
     6241 attgcagcca atggtttagc tggtctcacc ctagttggta aggcactttt tgagattgat
     6301 ccttctttga taggctattt gattaatttg cctttggtct tgttgggagc tagatgtttt
     6361 gggaaaagag ccatggttta cacagtaatg ggtattttgt cactttactt ttttgtgtgg
     6421 ctgtttcaac gctttccttt ggtcgttgac ttggaccatg acaatttagt cgtttccttg
     6481 atggctggaa ttattggtgg tctaggtggc ggtcttgtgt ttcgcaatgg tggaaccatc
     6541 ggtggagcag atattattgc taagttatta gaagacaagt taggactaca gctgaatcaa
     6601 gctcttttag gcattgatct ttttgtcatg attgtctctt tgacttatat ttccctgccg
     6661 caaatgatgt atgctctgat tgctagcttt atgtatggtc aaattgttcg cttggtacaa
     6721 caaggtggtt attcagcccg tggcattttt atcgtttcag aacaagcgga gaaaattgcc
     6781 cggttcatca tggacgaatt gggaagagga gttacttatc taacaggtga aggagcctat
     6841 tctggtcgcc gtaaaaaagt gatttatgtg gctttggggc gaagagatat tagggaatta
     6901 aaagcttttt taactcaggt tgatcccaat gcctttatca cttattttga tgttaatgaa
     6961 gtcaatagcc cagaattttt aactattaaa agcaaatacc ataaaaagag gaaataaatg
     7021 agcattagag ttgcctgcga agaatagtga ggataaagga tggcacatca cgataaattg
     7081 actaaactac taaaactgtt tttgattgcc ctaggtgtag ccatttatac cttcggtttt
     7141 gttaatttta acatggctaa tgctttggct gaaggtggtg tggcaggaat aaccttgatt
     7201 ttaaacgcgc attttgggat taatcctgct tactcttctc tcttatttaa tcttccactt
     7261 tttattttag gggcaaagat ttttgggaag cgttctttgg ctctaaccat ctatggaaca
     7321 gttctgatgt ccgcttttat ctggatgtgg caaaaagttc ctatcgaact tggcttggaa
     7381 aatgacatga tgttggtggc tgtagtggca ggtctctttt cagggattgg gagtggcatt
     7441 gtttttcgct atggtgcaac cacaggtgga acggatatta ttggtcgcat tgcagaagaa
     7501 aaatttggag ctaagctagg tcaaactttg cttttggtgg atgctcttgt attgacagct
     7561 tcgttgactt atgtggattt aaaacacatg ctttatactt tggtggcaag ctttgttttt
     7621 agccaaatga ttagtgtggt tcaaaatggt ggttacacta tccgtggaat gattattatt
     7681 accaagcatt cagaggctgc cgctcaagct atcctaaccg aaatcaatcg tggagtgact
     7741 tacttgaaag gtcaaggagc ttactctggt aatgattaca atatcatgta tgtgaccttg
     7801 aacccaactg aagttcggga agtcaaacgt attttagctg gtttggatcc agatgccttt
     7861 atctccatta ttgatgtgga cgaagttatt agctctgatt ttaaaattcg ccgaagaaat
     7921 tatgataaat aaaaatacag gtcaaacagc ctgtattttt attttggtcc cccacttaag
     7981 aaactaccaa gcataatagc tgatatagca gttcccttgt aatggcaagc ttttgttttt
     8041 accaagcgac taagatcaaa tatgctttta ggagcatcaa attgatgtgt taagatgtat
     8101 tgattgataa gtgagtcaat ttttgtagct agtagtgagc tcatcccctc tttttcaagt
     8161 tgactggcga gctcgaagat cgcttgacga aaataatcat ctgcttgtat tacttgttcg
     8221 ctataaactt tactaagagc atctaatatt tctttttctg atggcatggc attctcctgt
     8281 aacatattgt gctattatta taatataaaa aactaagact gtcataaatg tgacagcctt
     8341 agtgatgtta ttacttacat ttcatttggt gcgtcaacac cgagtaagcg aagcgcttct
     8401 ttaagaacag tagcagttgc atagcagaga gctaagcggt tatcacgttc tgagttgtcg
     8461 tcaagaatgc gagtgtgagc ataatatttg ttaaagcttt gggctaagtt aatagcaaat
     8521 ttagccataa tagaaggctc aaagttatct gatgttcgct tgataatgcg tgggaaatct
     8581 tggataagtt tgataatttc ccagctttca gcatcagcta aactgtaagt ggtggttgca
     8641 gatggcgtaa agtcagcttt acgtagaata gattggatac gagcgtgagc gtattgaaca
     8701 taaggtccag tttctccttc aaaggaaacc atggtttcaa ggtcaaagtc atacccattc
     8761 atacgatctg ttttgagatc gtaaaacttg atggcaccga caccaacagc gtgtgcaaca
     8821 gcttctttat cagcaagatt tggatttttc gcttcaattt gagaagcagc gcggttaatg
     8881 gcttcagcaa ccgtaggttc aagaagaata acattacctt tacgtgttga taatttagca
     8941 ccgccttttg tgacaagtcc aaaagcaacg tgtgtcatat catctgacca atcgtaaccc
     9001 atttcttgta gcactgcttt taactgtttg aagtgagcag cttgctcatt accaacaaca
     9061 taaacggatt tagcaaagtc ataagtgcgt ttacggtaaa gtgcggcagc taggtcacgg
     9121 gtaatgtaaa gagtagcgcc atctgatttt ttgatcaagg cagggtgttc aatgccgtat
     9181 ttttcaaggt taacaacttg ggcacctttg gattctacaa gaaggttttt cgcttctaat
     9241 agctcaagaa cctcatccat tttgtcatta tagaaggcct caccgttgta actatcgaag
     9301 gtgacatgta attggtcata aagacggtta aactcaagca aactctcatc acggaaccat
     9361 tgccataatt cggtagcttc tttgtcgcca tcttccaatt tacggaacca ttcacgagct
     9421 tcttcatcga cggttggatc agtttcagct tcagcattga tacgaacgta aagttttaag
     9481 agttcatcaa taggatgtgc ctgaacagca gcttcgtctc cccatttttt ataggcaaca
     9541 atgagcatac caaattgttt accccagtca ccaaggtgat tgatttttac aggttgataa
     9601 cccattttgg caaaaatgtg agcgagacta tcaccaataa ctgttgagcg aaggtgaccg
     9661 attgaaaatg gtttagcaat gtttgggctt gacatatcaa tagcaacatt tcgcccttga
     9721 ccttcgtctt gctgagcata gtctgaacct gctgtaatca cttgttcaag gacctgtgag
     9781 gaaatcttag ctttatcaag gaaaaaattg atgtaagggc cgacagctac gactttttca
     9841 aattggctct catcaatctg ttcagctaac tcgctagcaa tcatttgagg ggctttgcga
     9901 aggacttttg ctaagctaaa agctggaaag gcaaggtctc ccatatcaga attttttggg
     9961 gtttcaagca agttaaaaat agcatcttgt tctaattctg gaacaacttt agctatttca
    10021 cttgcgatta gagttttagt atccatacag cgtctcctaa aaattgtttg tcaccctatt
    10081 ctaacatatt tctttaaaat aaacgagga
//
LOCUS       DAUELZ010000060         9828 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.061, whole genome shotgun sequence.
ACCESSION   DAUELZ010000060 DAUELZ010000000
VERSION     DAUELZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9828
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.061"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..425
                     /locus_tag="VHI09_001156"
     CDS             <1..425
                     /locus_tag="VHI09_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ6121037.1"
                     /translation="STPEEPAAPSPSPESEDSSVAATTSPSPSTPAESETQTPPAVTK
                     DSDKPSSAAEKPAASSLVSEQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVMAHESG
                     KYLPSTGEKAQPLFIATMTLMSLLGSLLVTKRQKETKK"
     gene            677..1597
                     /gene="lmb"
                     /locus_tag="VHI09_001157"
     CDS             677..1597
                     /gene="lmb"
                     /locus_tag="VHI09_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEQ6121038.1"
                     /translation="MKKGFFLMAMAVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            1610..4087
                     /locus_tag="VHI09_001158"
     CDS             1610..4087
                     /locus_tag="VHI09_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEQ6121039.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGSATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YMMPKDGKNYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQAD
                     KSNENQQPSEASKEEEKEKESDNFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIF
                     QPEGVQFYNKNGELVTYDIKTLQQINP"
     gene            4257..4457
                     /locus_tag="VHI09_001159"
     CDS             4257..4457
                     /locus_tag="VHI09_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ6121040.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(4528..5154)
                     /locus_tag="VHI09_001160"
     CDS             complement(4528..5154)
                     /locus_tag="VHI09_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121041.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIIISHDFDMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(5138..5941)
                     /locus_tag="VHI09_001161"
     CDS             complement(5138..5941)
                     /locus_tag="VHI09_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121042.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIKQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFHQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGGGEQLQTEFARRLWRTLPQQDFLKGVTHDLRG"
     gene            complement(5953..6774)
                     /locus_tag="VHI09_001162"
     CDS             complement(5953..6774)
                     /locus_tag="VHI09_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121043.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVFLATVFGVLAGLGNSFID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(6771..7748)
                     /locus_tag="VHI09_001163"
     CDS             complement(6771..7748)
                     /locus_tag="VHI09_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121044.1"
                     /translation="MKRTTIIIIWKIIRCITLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(7861..9489)
                     /locus_tag="VHI09_001164"
     CDS             complement(7861..9489)
                     /locus_tag="VHI09_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ6121045.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDEIV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKTLGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESTK"
     gene            9666..>9828
                     /locus_tag="VHI09_001165"
     CDS             9666..>9828
                     /locus_tag="VHI09_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121046.1"
                     /translation="MTYFRLKKELNWSLRSKGIVVYEGNQKLQIAINTGKNNSEKVFF
                     DTFLEVVRAA"
BASE COUNT         3252 a         1917 c         1921 g         2738 t
ORIGIN      
        1 catcaactcc agaagagcca gcagctccat ctccatctcc tgagagtgag gattcttcag
       61 tagcagctac gacaagcccg tctccatcaa ctccagctga atcagagact cagacgccac
      121 cagctgttac taaagactct gataagccat cttcagcagc tgaaaaacca gcagcctctt
      181 cacttgtttc agaacaaacc gttcaacaac caacttcaaa gagatcttct gataaaaaag
      241 aagagcaaga acagtcttac tctccaaatc gctcattgtc aagacaggtt atggcccatg
      301 agtcaggtaa gtacttgcct tcaacaggtg aaaaagcaca gccactcttt atagctacta
      361 tgactttgat gtctctactt ggcagtcttt tagtcacaaa acgccaaaaa gaaactaaaa
      421 aatagtatta cttaaactga taagagctta tttaatagag agaaccaatt tggttctctc
      481 tattttttta tatgtgctgc ctcatcaaaa agaagttagg acaaatagct gtcaacaatt
      541 accttttgct tatcccttca gttgctgtga ttttatcata cagtgataaa agatggtagt
      601 tgccattgac aaagcattga taaaggagta aaattaactg gttaataact ggttaaatta
      661 taattgagga ggtactatga aaaaaggttt ttttctcatg gctatggctg tgagtttagt
      721 aatgatagca gggtgtgata agtcagcaaa ccccaaacag cctacgcaag gcatgtcagt
      781 tgtaaccagc ttttacccaa tgtatgcgat gacaaaagaa gtatctggag acctcaatga
      841 tgtgaggatg atccaatcag gtgcaggcat tcattccttt gaaccgtctg taaatgatgt
      901 ggcagctatt tatgacgcgg atttgtttgt ttaccattca catactttag aagcttgggc
      961 aagggatcta gaccctaatt taaaaaaatc aaaggttgat gtgtttgaag cgtcaaaacc
     1021 tctgacacta gatagagtca aagggctaga agatatggaa gtcacacaag gcattgaccc
     1081 tgcgacactt tatgacccac atacctggac agatcccgtt ttagctggtg aggaagctgt
     1141 taatatcgct aaagagctag gacgtttgga tcctaaacac aaagacagtt acactaaaaa
     1201 ggctaaggct ttcaaaaaag aagcagagca actaactgaa gaatacactc aaaaatttaa
     1261 aaaggtgcgc tcaaaaacat tcgtgacgca gcacacggca ttttcttatc tggctaaacg
     1321 attcggcttg aaacaacttg gtatctcggg tatttctcca gagcaagagc cctctcctcg
     1381 ccaattgaaa gaaattcaag actttgtcaa agaatacaac gtcaaaacta tttttgcaga
     1441 agacaatgtc aatcccaaaa ttgctcatgc tattgcgaaa tcaacaggag ctaaagtaaa
     1501 gacattaagt ccacttgaag ctgctccaag cggaaacaag acatatctag aaaatcttag
     1561 agcaaatttg gaagtgctct atcaacagtt gaagtaaagg agattattag tgaagaaaac
     1621 atatggttat atcggctcag ttgctgccat tttactagct actcatattg gaagttacca
     1681 acttggtaag catcatatgg gttcagcaac aaaggacaat caaattgcct atattgatga
     1741 tagcaaaggt aaggcaaaag cccctaaaac aaacaaaacg atggatcaaa tcagtgctga
     1801 agaaggcatc tctgctgaac agatcgtggt caaaattact gaccaaggct atgtgacctc
     1861 acacggtgac cattatcatt tttacaatgg gaaagttcct tatgatgcga ttattagtga
     1921 agagttgttg atgacggatc ctaattacca ttttaaacaa tcagacgtta tcaatgaaat
     1981 cttagacggt tacgttatta aagtcaatgg caactattat gtttacctca agccaggtag
     2041 caagcgcaaa aacattcgaa ccaaacaaca aattgctgag caagtagcca aaggaactaa
     2101 agaagctaaa gaaaaaggtt tagctcaagt ggcccatctc agtaaagaag aagttgcggc
     2161 agtcaatgaa gcaaaaagac aaggacgcta tactacagac gatggctata tttttagtcc
     2221 gacagatatc attgatgatt taggagatgc ttatttagta cctcatggta atcactatca
     2281 ttatattcct aaaaaagatt tgtctccaag tgagctagct gctgcacaag cttactggag
     2341 tcaaaaacaa ggtcgaggtg ctagaccgtc tgattaccgc ccgacaccag ccccaggtcg
     2401 taggaaagcc ccaattcctg atgtgacgcc taaccctgga caaggtcatc agccagataa
     2461 cggtggctat catccagcgc ctcctaggcc aaatgatgcg tcacaaaaca aacaccaaag
     2521 agatgagttt aaaggaaaaa cctttaagga acttttagat caactacacc gtcttgattt
     2581 gaaataccgt catgtggaag aagatgggtt gatttttgaa ccgactcaag tgatcaaatc
     2641 aaacgctttt gggtatgtgg tgcctcatgg agatcattat catattatcc caagaagtca
     2701 gttatcacct cttgaaatgg aattagcaga tcgatactta gccggccaaa ctgaggacaa
     2761 tgactcaggt tcagatcact caaaaccatc agataaagaa gtgacacata cctttcttgg
     2821 tcatcgcatc aaagcttacg gaaaaggctt agatggtaaa ccatatgata cgagtgatgc
     2881 ttatgttttt agtaaagaat ccattcattc agtggataaa tcaggagtta cagctaaaca
     2941 cggagatcat ttccactata taggatttgg agaacttgaa caatatgagt tggatgaggt
     3001 cgctaactgg gtgaaagcaa aaggtcaagc tgatgagctt gctgctgctt tggatcagga
     3061 acaaggcaaa gaaaaaccac tctttgacac taaaaaagtg agtcgcaaag taacaaaaga
     3121 tggtaaagtg ggctatatga tgccaaaaga tggcaagaac tatttctatg ctcgtgatca
     3181 acttgatttg actcagattg cctttgccga acaagaacta atgcttaaag ataagaagca
     3241 ttaccgttat gacattgttg acacaggtat tgagccacga cttgctgtag atgtgtcaag
     3301 tctgccgatg catgctggta atgctactta cgatactgga agttcgtttg ttatcccaca
     3361 tattgatcat atccatgtcg ttccgtattc atggttgacg cgcgatcaga ttgcaacaat
     3421 caagtatgtg atgcaacacc ccgaagttcg tccggatgta tggtctaagc cagggcatga
     3481 agagtcaggt tcggtcattc caaatgttac gcctcttgat aaacgtgctg gtatgccaaa
     3541 ctggcaaatt atccactctg ctgaagaagt tcaaaaagcc ctagcagaag gtcgttttgc
     3601 aacaccagac ggctatattt tcgatccacg agatgttttg gccaaagaaa cttttgtatg
     3661 gaaagatggc tcctttagca tcccaagagc agatggcagt tcattgagaa ccattaataa
     3721 atctgatcta tcccaagctg agtggcaaca agctcaagag ttattggcaa agaaaaacgc
     3781 tggtgatgct actgatacgg ataaacccaa agaaaagcaa caggcagata agagcaatga
     3841 aaaccaacag ccaagtgaag ccagtaaaga agaagaaaaa gaaaaagaat cagataactt
     3901 tatagacagt ttaccagact atggtctaga tagagcaacc ctagaagatc atatcaatca
     3961 attagcacaa aaagctaata ttgatcctaa gtatctcatt ttccaacccg aaggtgtcca
     4021 attttataat aaaaatggtg aattggtaac ttatgatatc aagacacttc aacaaataaa
     4081 cccttaacca aaagaagatc tcattgttaa agcactgctt tgtcaaagta agttacggtg
     4141 attttgaagt cattctatgt aacgagtagt gataaaagtt ggataatagt ggttttcttt
     4201 tgcaaagaaa tggtatccat gttagaataa taaaaaaaaa gaggaggatt ctaatcatgt
     4261 cagaagaaaa attaaaatca aaaattgagc aagcatctgg tggcctaaaa gaaggtgctg
     4321 gtaagttgac aggtgataaa gagctagaag caaaaggatt tgttgaaaaa acaattgcta
     4381 aaggcaaaga acttgcagac gatgcaaaag aagcggttga aggtgctgta gatgccgtta
     4441 aggaaaaatt aaaataatta aaaaaccagc tgtaagctgg tttttttaat ggtgtgattt
     4501 tgttagaaaa agactcaata ggtaatgtta ggtatgcttg aacagttgag tacgattctc
     4561 ttcaatcata tagcacgcgt cacaaagttt ttctaacata tcaaaatcat gactaataat
     4621 gatgagacct aaatttctat cctttacaat ctccaacagg cttttccata cactagcttg
     4681 tgtaatgcta tccaacatag tagtcatttc atctgcaata aggtatttcg tctctggatg
     4741 taatgaacgc acaatcgaaa agcgttgcaa ttcccctccc gagagttcac taggacgacg
     4801 ctttagccat ttttcttgaa tgccaaaagc atctcgcaaa tcttgacttg gatagtaggc
     4861 ttcttccaaa ctttttttca taggccataa aggattcatg gtttgttcag gatgttgttg
     4921 gataagttgt acaggtctaa atgctttgtt aggcaaatga ctgccatcaa ctaatacttc
     4981 accagattta ggctgcaaaa aaccggcaag cactctggat aaactcgttt ttccacaccc
     5041 actttgtcca aatatcccta agatttgacc aggtgctacc tctaaatcaa tctccttaaa
     5101 aagccattga tcttttttat gataaaagcc aagcttttta gcctctaagg tcatgagtaa
     5161 ctcctttcaa aaagtcttgc tgagggagtg tgcgccataa acgtctcgca aattccgttt
     5221 gtaattgctc tccacctccg ctaaaaaagc tagctggggc tgtttcaata gcttttccct
     5281 ctttaaaaat agttatacga tccgcaattt gactagctgc tacaatatca tgagtgataa
     5341 agatgacgct tattccttta tctgcaaaag agcgtagttg atctaaaacc atttgcagag
     5401 catctggatg taatccaggg gtgggctcat ccgcaataat caaagaaacc gtatcactaa
     5461 tacacgttgt aaacaaaaca cgtcggagca ttccgccaga aagttggaaa ggatacaagt
     5521 caccatcact ttcttttaaa ccaaactgat gaaataatcc ttcttgagta gccttagcat
     5581 tttctgagat acccaagcgc acctgatgct tgactttcat agatggatct aaataattaa
     5641 cggattgtgg aatcaacgtc atttcttttc ctcgcaactg tttaatgcgt ttagaagtta
     5701 gtgattgacc acgataaatc atatctcctg ttacagctgc atttttagga agaatatcca
     5761 taatagcatg tgctaataaa ctcttgcctg aaccactagc acctataata gctaataact
     5821 cacctttttt aacttctaaa ttcagcgctt ggatcggtgt ggattgaaat ggttttaaaa
     5881 atcttccgta ttgagtgaag gtgatggaga ggtctttaat gcttaataat gtttctgtca
     5941 tatctctacc tcctaaaaat gatccgtttg agggtaaaag agtttcttta aagattctcc
     6001 gatagtatca aaggcattga caaccaaaat aagataaagg cctggaaaaa tcaccaacca
     6061 ccaattgcca agagagatat gcttagctgc ctctgacaaa atgataccaa ccgaaggttg
     6121 ttcggcagaa aggccaaatc ctaagaaagt catggatgct tcatgcaaga tgacgtgcgg
     6181 aaataagagg ataaacccaa tgaaaatttg agaagcaatc aaaggcagga tatgatgcct
     6241 cacaatataa taaggcgttt ttcccatgct tttagagagc tggacaaagg ctttattctt
     6301 tagatcatag acttcattgc ggataagcct tgctagagag ggccaatggg taacagctgt
     6361 tgcaatgata accccttgag cccctttccc aacaacaaaa gaaatgagaa tcataaaaat
     6421 caaatgaggc ataccaataa acaaatcaac caaccaggct attattttat caataaagct
     6481 atttcctaga cctgcaagca ctccaaaaac ggtcgcaaga aagactccca taagggcacc
     6541 taataagccg acttgtaaag agaaataaag ccctttaatc gttctgacaa acatatccct
     6601 acctaaacca tctgttccaa aaagatggtt taatgaagga gcgaggttgc gtaaagctgc
     6661 attggtttcc aaaggcgtcc tatagaaata aagatttaag gctagaatac taagaatgag
     6721 agaaatggcg atacctagtt gccataaaac catcgttcga cgtttcaata tcatactttt
     6781 ctccttaact gtggattgat gatgctattt aaaatgtccg caataagatt gcccgcaaaa
     6841 acaaataatg ttcctatcat cacgatagct agaagtagcg gtgtatcact tttaagtcct
     6901 gcttcagtga gggtagaccc taagccagga tatgagaaaa cttgctcagc aagaacagat
     6961 cctccaaaca attctccaaa ataggaaaaa tgcagtgtaa tagctggtac gatagcattt
     7021 ctaaggcaat gatgtttaaa aatttgccac tgtgtttccc cacgcgctct ggcaaataag
     7081 acatattcac tagaaagcac cgacatcatt ttagttctcg tatgaagagt gacattggca
     7141 atgcctagaa tacttagcgt gaaaacaggt aacataaggt gcttaatccg atcagctaac
     7201 gtaatatctt gactcaaagt gcctatcggg gaagaaatac caatcgggaa ccaccccagc
     7261 tggacagaaa agattaataa aaaaatgagg ccaatccaaa acgttggtac tgatatctga
     7321 aggtaagaaa accacctgac aactcggtca agtaatttcc cttgatggaa agctgataac
     7381 gttcctaaga taaatccaat aagacccgat aagatccaag agagtcccat aagtatgaaa
     7441 gaagcacctg cccgtgatct aataatatca ctaacaggtt gccgataaac gagcgaggtc
     7501 cctaaatgtc cctgtatcac atttttcaac caaataaaat attggactag agctggctta
     7561 tccaagccat agtggtgagc aatcgctttg tactgagcag gggttagtga tgtgtcataa
     7621 ttgacacttg ccatgacggg atctactgga gattgtttta acaaaacgaa ggtcaaaaca
     7681 gataccccaa aaataagtgt gatacatctg atgattttcc agatgataat aatggtagta
     7741 cgtttcacaa tgaaatctcc ttaatcgata agaaaaggag ccgaggaaac gatcagcaca
     7801 gttttgtgta tcttgttttc tcagactcca aacttaaagt cgctgtatta actaaacgcg
     7861 ttacttagtt gattcatccc aagtccactc ggcaatgtta gtcaataatg accaatcatg
     7921 accatgactg tggacgcctt gtttacctac attgatacgt ttatcaccaa tataagtatg
     7981 gttaaggctc accaaccata catttggcaa atctccaaga gtagaagcac ctgttttgcc
     8041 atcccactgc gctaacttcc aatattcgtt agctttgtca aggtcagaag atgtcattgc
     8101 tttgtcaagg tacttagtca cggtaggatt gttataaaac gtaatattgg tccaaccttt
     8161 acctgctagg cttggatgat gtgattcata aaattgctgc gcgtgatgac gtcctccggc
     8221 ataaagtaag gctgagtcat gtgacttcgt tgccatttca tcccagttac tagctttgag
     8281 tttaatagta atccctaggg ttttggcttg ctctgctact tcaacggcta agttcgctcg
     8341 caattgatca ttagtagggt agtacagatc aaacgctgca tcaaggtcac cttttttacg
     8401 gctaccgtct gcttgttctt tccatcccgc ttttgtcaac aattgcttag ctttagctac
     8461 tttattatct ttaatggctg tttttggatt ccaaaatggt gttttatcaa taattgaata
     8521 agctggttta ccataaccat ttaaaaccgt atcgagaact ttttgtctat ttaaaccaat
     8581 agtcaaggct ttgcggattg ctggatcact agtgacatca tttcctacag gataaccatc
     8641 aggagaatca gtgatgacgc cttttttcac ataaggtaat gataagccgc gcacatcatt
     8701 tgatggaatg tcaaggaggc gagtgccttt gactttctta ctagcaagtt ctggtgtcgc
     8761 gtagatcata tcaacatcac cagattctaa agcagctagt gctgtgtttt catcaagtaa
     8821 gacccaagtc cactttttaa agtatggttt tttcccatgc caataagggt tacgaacaaa
     8881 aatagcttgt tctccagcct tatattcttt taccatgtaa ggtcctgaac cgataggatt
     8941 gctcttatac ttatcattgt aatgtttttt agggacgatt gggatttcag tcaactgtgc
     9001 tgtaaatgtc gaatgcgcct cagtcaaatg gatattgacc tgatttttcc caactacttc
     9061 aacgttcttg atgaaggtta gatcccaagc ttttccatct gctttcaaca tatcataagt
     9121 aaacttaaca tcatcagcag taacaggctc accatttgag aatttaaaat catcatgcaa
     9181 atcaaacgac caagtcagcc catcttcaga gagatggtat gttttagcaa gctctccttt
     9241 tatatctagt tcaggagaac gtttcaatag agtgctatga gtgatattcc cttcattgtg
     9301 gactccataa cggtcctttg gatcgaattc atgagggagc tttgccccca tagaaacgac
     9361 aatttcgtct tttggacgtt gtttgcgctg acgttctttt gtttgaggct tttgttgttg
     9421 acatgcaact aaaataagcc cagtcaaaaa taacgtgata atagagaagt attttaggta
     9481 ttttgacaca atcaaatctc cttatttcta ataataaata ataatagtaa cgactccatc
     9541 tcattatagc aataaacaaa taggagggca attgtttaac tggttaatta gaatttttgt
     9601 tattttcagg aagatactca ggttttttaa taagttgatg tattaacaaa agataaagag
     9661 gtttcatgac ttattttaga ttaaaaaaag aattaaactg gtcactaaga agtaaaggaa
     9721 ttgtagttta cgaagggaat caaaaacttc agatagccat taatacaggt aaaaataaca
     9781 gtgaaaaagt cttttttgat acttttcttg aggtggttcg ggcggcag
//
LOCUS       DAUELZ010000061         9717 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.062, whole genome shotgun sequence.
ACCESSION   DAUELZ010000061 DAUELZ010000000
VERSION     DAUELZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.062"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            42..746
                     /locus_tag="VHI09_001166"
     CDS             42..746
                     /locus_tag="VHI09_001166"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEQ6121047.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKGWFAQIGLNLIDFKLEFGFDQEGTIILADELSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            870..4595
                     /locus_tag="VHI09_001167"
     CDS             870..4595
                     /locus_tag="VHI09_001167"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEQ6121048.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDRLLTEAEITSELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFENYKADDFAAYKADQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKFPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGEVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPIKYQGLNGTEIAISESQERMSVVVRPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGETIVDLERRFLDTNGVRVVIDAKVVDKDLTV
                     PEARTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTRTVSVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKILSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFEAIQAQHKIT
                     AASAAKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADAVKIG
                     QTQADFTVTVNGNDLAGASLLAAFEGKFEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEIAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANANSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQTLFASAVKYFTGK"
     gene            4756..6210
                     /locus_tag="VHI09_001168"
     CDS             4756..6210
                     /locus_tag="VHI09_001168"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEQ6121049.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSNFMGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAVDEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            6238..7260
                     /locus_tag="VHI09_001169"
     CDS             6238..7260
                     /locus_tag="VHI09_001169"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEQ6121050.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGVLGGFG
                     GMFDLSKTGVKEPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKLEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVKAALPLIKEELVNGIGHITGGGFIENIPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            7428..7982
                     /gene="purN"
                     /locus_tag="VHI09_001170"
     CDS             7428..7982
                     /gene="purN"
                     /locus_tag="VHI09_001170"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEQ6121051.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFLVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLAGYMKIVGETLLLAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            8166..9713
                     /gene="purH"
                     /locus_tag="VHI09_001171"
     CDS             8166..9713
                     /gene="purH"
                     /locus_tag="VHI09_001171"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEQ6121052.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDVDSHLQAAKDNNIELIDLVVINL
                     YPFKETILRPDVTYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVIALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEVAAAGVKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
BASE COUNT         2783 a         1892 c         2293 g         2749 t
ORIGIN      
        1 gttactctta aatgaaaaca gaaagaaagg cgaataatca agtgactaac cagcttattt
       61 ataaaggaaa agcgaaagat atttatagta caaaagatga aaatgtaatt agaacggtct
      121 ataaagacca agctactatg ttgaatggag ctcgtaagga gactattgat ggcaagggag
      181 cactaaataa tcagatttca tctcttattt ttgagaaatt aaataaggct ggtgtggtga
      241 cacattatat tgagcaaatt tcaaaaaacg aacaattgaa taaaaaagtt gatattattc
      301 cacttgaggt ggtgttacgg aatgtgacag caggctcatt ttcaaaacgt tttggagtgg
      361 aagaaggacg tgttcttgaa acgccaattg tggaatttta ctacaaaaat gatgatctga
      421 atgatccttt tatcaatgat gagcatgtga agtttttggg tattgttaat gatgaggaga
      481 ttgcttatct gaaaggagag actcgccgta ttaatgaact cttgaaaggt tggtttgctc
      541 agattgggct taacttgatt gacttcaagt tggagttcgg atttgatcaa gaaggtacaa
      601 ttatcttagc agatgaactt tcgcctgata actgtcgcct atgggataag aatggtaacc
      661 atatggacaa ggatgttttt agaagagacc ttggcaatct gacggatgtc tatcaagttg
      721 tacttgagaa gttaatagca ttgtaactag aagaggaagg aattgaatcc gttcgggaaa
      781 ctccgaaaaa aagatggaac ttactgaaat ctcaatttca gatgtcctcc tattttccag
      841 tcgctccctt gtcggatttg gtatcttata tgaataaacg tatttttgtt gagaaaaagg
      901 ctgactttgg cattaaatcg gctagccttg tgaaagagtt gacacataat ctacaactga
      961 cttctttgaa ggacttgcgt attgtgcagg tctatgatgt cttcaatttg gctgaggatt
     1021 tactggcgcg tgctgagaag catattttct ctgagcaggt gacagaccgt cttttgacgg
     1081 aagctgaaat cacttcggag cttgataagg ttgccttctt tgccattgag gcgcttcctg
     1141 gtcaatttga ccaacgtgct gctagttcgc aagaagcttt gctattattt ggaagtgaca
     1201 gtcaggttaa ggtcaataca gcccagcttt acttggtcaa taaggatatt gcagaagcag
     1261 agcttgaagc cgttaagaac tatcttttga accctgttga ttcgcgtttc aaggacatta
     1321 ctttgccgct tgaagagcag gctttctctg tatctgataa gacgatccct aatcttgatt
     1381 tctttgaaaa ttataaagct gacgattttg cggcctataa ggcagatcag ggcttggcta
     1441 tggaggtcga tgaccttctc ttcatccaag attatttcaa atcaatcgga cgtgtgccaa
     1501 ctgagactga gttgaaagtt ttggatactt actggtcaga ccactgccgt cacacaacct
     1561 ttgaaactga attgaagaac attgattttt cagcttctaa attccaaaaa caattgcaga
     1621 caacttatga caaatatatc gccatgcgtg atgagcttgg tcgttctgaa aagccacaaa
     1681 cacttatgga tatggcgact atttttggtc gttatgagcg tgccaacggt cgtctggacg
     1741 atatggaagt ctcagatgaa atcaatgcct gctcagttga gattgaagta gatgttgatg
     1801 gtgtgaaaga gccttggctc ctcatgttta agaacgagac tcacaatcac ccaacagaaa
     1861 ttgagccatt cggtggagcg gcgacttgta tcggtggtgc tattcgtgac ccattgtcag
     1921 ggcgttcata cgtttatcag gctatgcgta tttcaggcgc aggcgatatc acgactccga
     1981 ttgcggaaac acgtgctggt aaatttccac aacaagttat ttctaaaact gcggcgcacg
     2041 gctattcttc atatggtaac caaattgggc ttgcgacaac ttatgtgcgt gagtacttcc
     2101 accctggctt cgtagccaaa cgtatggagc ttggagctgt ggtcggagcg gcacctaagg
     2161 aaaatgtggt tcgtgaaaaa ccagaagcag gtgatgtggt aatcttgctt ggtggtaaaa
     2221 cagggcgtga cggtgtcggc ggtgcgacag gttcatctaa ggttcaaacg gttgagtctg
     2281 tggaaacagc tggcgcagag gtacaaaaag ggaatgccat cgaagagcgt aagattcaac
     2341 gtctcttccg tgatggtgaa gtgactcgtc tcatcaaaaa atcaaacgac tttggtgctg
     2401 gtggtgtctg tgttgctatc ggtgaattgg ctgacggtct tgaaatcgat ttggacaagg
     2461 tcccaattaa ataccaaggt cttaatggta ctgaaattgc aatctcagaa tctcaagagc
     2521 gtatgtcagt cgttgttcgt ccaagtgatg tggatgcctt catcgcagct tgcaacaagg
     2581 aaaatatcga tgccgttgta gttgcgacag ttaccgagaa accaaatctt gtcatgactt
     2641 ggaatggtga aaccatcgtt gacttggaac gccgtttcct tgataccaac ggtgtccgtg
     2701 tcgttattga tgctaaagtc gttgacaagg acttgacagt tccagaagca cgcacaacat
     2761 cagcagagac acttgaagca gatacgctta aggtcttgtc tgacctcaac cacgctagtc
     2821 aaaaaggtct tcaaactatc tttgactcat ctgttggtcg ctcaaccgtt aaccacccaa
     2881 tcggtggtcg ttaccaaatc acaccgacag aaagttctgt tcaaaaattg ccagttcaac
     2941 atggtgtgac aagaacagta tctgttatgg ctcaaggtta caatccttat attgctgagt
     3001 ggtcacctta ccacggtgct gcctatgctg tcattgaagc gacagctcgc ttggtagcaa
     3061 cgggtgctga ctggtctcgt gcacgtttct cttaccaaga gtactttgag cgtatggata
     3121 aacaggcaga gcgttttggt cagccagtat cagctcttct tggttctatt gaggctcaga
     3181 ttcaacttgg tttgccatca atcggcggta aggactctat gtctggtact ttcgaagact
     3241 tgacagtacc accaaccttg gtagctttcg gtgtgacaac agcggacagc cgcaagattc
     3301 tctctcctga gtttaaagcg gctggcgaaa acatttacta tatcccaggt caagctattt
     3361 cagaagatat tgattttgat cttatcaagg ctaactttag ccagtttgag gctattcaag
     3421 ctcaacataa gattacagct gcctcagccg ctaaatacgg tggtgtccta gaaagtcttg
     3481 ctctcatgac ttttggtaac cgtatcggtg cttctgttga aattgcagag cttgacagca
     3541 gcttgacagc tcaactcgga ggttttgtct ttacatcagc tgaggaaatt gctgacgcgg
     3601 tgaaaatcgg tcaaactcag gcagacttta cagtcactgt caatggaaat gaccttgctg
     3661 gcgctagcct tctagcagct ttcgaaggca aatttgaaga ggtttaccca acagaattcg
     3721 aacaggcaga cgctcttgaa gaagttcctg ctgtggtatc agatactgtt atcaaggcta
     3781 aggaaacaat tgaaaaacca gtggtttaca ttcctgtctt ccctggtacc aactcagagt
     3841 atgattcagc taaggccttt gaacaggttg gagctagtgt caacttggta ccatttgtaa
     3901 ccttgaatga gattgctatt gctgagtcag ttgacactat ggttgctaat attgctaagg
     3961 caaatatcat cttctttgct ggaggcttct cagcagcgga tgaaccagat gggtctgcta
     4021 agtttatcgt caatatcttg cttaacgaga aggtccgcgc agctattgac agcttcatcg
     4081 aaaaaggtgg ccttatcatt ggtatctgta atggtttcca agcccttgtt aaatcaggtc
     4141 ttcttccata cggaaacttc gaggaagctg gtgagacaag tccaactctc ttctataacg
     4201 atgcgaacca acacgttgcc aagatggttg agactcgtat cgcaaatgcc aactcacctt
     4261 ggttggcagg agttgaggtc ggcgatattc acgccattcc agtttcacac ggtgaaggta
     4321 aatttgttgt cagcgcttct gaatttgcag agctaagaga caatggtcaa atctggagcc
     4381 aatatgtgga ctttgacgga caaccatcta tggattctaa atacaatcca aacggctctg
     4441 tcaatgccat cgaagggatt accagcaaga atggtcaaat catcggtaag atgggacact
     4501 cagaacgctg ggaagacgga ctcttccaaa atatccctgg taacaaagac caaaccctct
     4561 ttgcaagtgc tgtaaaatac tttacaggga agtaaaaagg ttcagtgaac ctttttagcc
     4621 ttcgccttga agtagtgaag ggcgaggtta gaaagaatct cgcttgattt ttgagaaata
     4681 gtaaacacgg gcgataagtt gtgcgaaaaa ctttctattt tcgctttact tgacagcctt
     4741 tgtatcgagg taaaaatgac atacgaagta aaatctctaa atgaagaatg tggagtcttt
     4801 ggtatctggg ggcatcctca ggcagctcaa gtcacttact ttgggcttca tagccttcaa
     4861 catcgcggtc aagaaggagc tggtattgtt tcaaatgaca atgggaaact ctatggttat
     4921 cgaaatgttg ggcttctttc tgaagttttt aagaatcaat ctgaattaga taatttaact
     4981 gggaatgcgg ctattggaca tgttcggtat gctactgcag gttctgcaga tattcgcaat
     5041 attcagcctt ttctttataa atttcatgac gggcaatttg ctttatgcca taatggtaat
     5101 ttgacaaatg ctatttcctt aaggaaagaa ttagaaaagc aaggtgcaat tttcaatgcc
     5161 tcctcagata ctgaaatttt aatgcatttg attcgtcgaa gccataactc aaatttcatg
     5221 ggaaaggtaa aagaagctct aaatacagta aagggtggct ttgcctatct actgatgaca
     5281 gaaaataaat taattgctgc tcttgaccct aatgcctttc gtcctttgtc aattggacaa
     5341 atgcaaaatg gtgcctgggt tatttccagt gagacctgtg cttttgaggt ggtaggcgca
     5401 aaatgggtta gagatgttga acctggcgaa gttattctta tagacgatag tggtattcaa
     5461 tgtgatcgtt atactgatga aacgcaactg gcaatttgtt cgatggaata tgtctatttt
     5521 gcaaggccgg attcaactat acatggtgtt aatgttcata cggctcgaaa aaatatggga
     5581 aagcgtcttg cacaagaatt taaacaggat gctgatattg taattggtgt cccaaattcg
     5641 tccttatcgg ctgctatggg ctttgctgaa gaatccggat taccaaatga gatgggtctt
     5701 gtaaaaaatc agtatacgca gcgaaccttt attcaaccga cacaagaatt aagggaacaa
     5761 ggtgttcgaa tgaaactatc agcggtatct ggtgttgtca aaggaaagcg cgttgttatg
     5821 attgatgact caattgtaag aggaacgact tctagaagga ttgtcggatt attaagagaa
     5881 gcaggagcta gtgaagtaca tgttgctata gctagtccag aattaaagta tccttgtttt
     5941 tatggtattg atattcaaac aagacgagaa ttaatttcag caaatcacgc tgttgacgaa
     6001 gtgtgtgata ttattggcgc agatagtctg acctatctct cattagatgg gttgattgaa
     6061 tcaattggac tcgaaacaaa agcaccaaat ggtggtttat gcgtagctta ctttgatggg
     6121 cattatccaa caccgcttta tgattatgaa gaagaatacc tcagaagttt agaagagaaa
     6181 acaagttttt atattcaaaa agtgaaataa gctctgctaa ttatataagg agaaaacatg
     6241 tctgaaaaaa atgcttatgc aaaatctggt gttgatgttg aagctggtta tgaagtggtt
     6301 gaaagaataa aaaaacacgt tgctcgtaca gaacgtgcgg gagttatggg agttctaggt
     6361 ggctttggtg ggatgtttga cttgagtaaa acaggagtta aagagcctgt cttggtttca
     6421 gggactgacg gtgtcggaac aaaacttatg cttgctatca agtacgacaa gcacgacaca
     6481 atcggtcaag actgtgttgc catgtgtgtc aacgatatta ttgcagcagg tgctgagccc
     6541 ctttacttcc ttgactacgt agcgacagga aaaaataacc cagtcaaact tgaagaagtt
     6601 gtttctggcg ttgcagaagg ttgtgttcaa gcaggagcag cactcattgg aggtgagacc
     6661 gctgaaatgc ctgggatgta tggcgaagat gattatgacc ttgctggctt tgctgttggt
     6721 gtggctgaaa aatctcaaat catcgacggt tcaaaagtaa aagaaggcga tattcttttg
     6781 ggacttgctt caagtggtat ccattcaaac ggttattctt tggtacgtcg tgtctttgct
     6841 gactatactg gtaaagagct gcttccagag cttgaaggca aacaacttaa ggatgtcctc
     6901 cttgagccaa ctcgtatcta tgttaaagca gctctgccat taatcaagga agaactagtc
     6961 aacggtatcg gccacatcac gggtggtggc tttatcgaga atattcctcg tatgtttgcg
     7021 gatgatttgg ctgcggaaat cgatgaggac aaggtgccag tgcttccgat tttcaaggcg
     7081 cttgaaaaat atggtgacat caagcacgaa gaaatgtttg aaatcttcaa tatgggtgtc
     7141 ggtcttatgc tagctgttag tcctgaaaat gttaaccgcg tcaaagagct cttggatgaa
     7201 ccagtttatg aaatcggtcg tatcatcaag aaagcagacg atagtgtggt gattaaataa
     7261 gtcactaaat tttataagaa aggagtgttt agttagtttg cctaactgaa cacgggatta
     7321 aatttctagg aaaaaagata aaatgactta gacgcaagcg tcgttgttcg ttttcctatt
     7381 tttctacgaa atttttcggc aagccgaatc atccctttgt atcgaatatg aaaatcgctg
     7441 tttttgcttc tggtaatggt tccaactttc aggtcatagc agagcagttt ctagttagtt
     7501 ttgtcttttc agatcatcgt gatgcctatg ttttagagcg tgctcagaat ttagccattc
     7561 caagcttcgc ttttgaactc aaggagtttg agaataaggc ggcttacgag caagccatcg
     7621 ttgatttgtt ggacaaacac gagattgact tagtctgtct ggcgggctat atgaagattg
     7681 ttggagaaac cttactcttg gcctatgagg ggcgtatcat caatattcac ccagcctacc
     7741 tgcctgaatt tccaggtgcc cacggtatcg aggatgcttg ggaagctggt gttgaccagt
     7801 ctggcgtgac catccactgg gttgactctg gtgtggatac cggtcaggtc atccaacaag
     7861 tgcgcgtgcc acgcctagca gacgatagcc tagaaagctt tgaaacccgt atccatgaaa
     7921 ccgaatatca actctaccca gctgtcttag atagcttggg agtggagagg aaagtaatct
     7981 agcctttatc ttaaaatttt aggtaaagtt aggaaagata gtaaagaaga attaacgttc
     8041 caaaaagaaa ggaattgaac ccgtctggaa ggcacagaaa aaagataaac atctcctagg
     8101 cgcaagcgtc ttcgtcgagt ttcctatttt tccttggcct tctagtcgga cttggtatct
     8161 taaatatgac taaacgtgct ttaatctcag tttctgataa gtcaggaatt attgactttg
     8221 caaaagaatt gaaaaacttg ggttgggata ttatctcaac tggtgggact aaggttgccc
     8281 ttgataatgc tggagttgag actattgcca tcgacgatgt gactggattc ccagaaatga
     8341 tggacggtcg tgttaagacc ctccatccaa acattcacgg tgggcttttg gcccgtcgcg
     8401 atgttgacag ccaccttcag gctgctaagg ataacaatat cgagctgatt gacctcgtgg
     8461 ttatcaacct ctatcccttc aaggagacta tccttcgtcc agacgtgacc tatgatttgg
     8521 cggtggaaaa tatcgatatc ggcggtccat caatgcttcg ctcagccgca aaaaaccatg
     8581 ctagcgtgac cgttgtggtt gacccagcgg actatgcgac tgttttggga gaattagctg
     8641 acgctggtca gacgacattt gaaacgcgtc aacgcttggc agctaaggtc tttcgtcaca
     8701 cagcagccta cgacgctttg attgctgagt acttcacagc tcaagtggga gaggctaagc
     8761 ctgaaaaatt aaccatcact tacgacctta aacaggctat gcgttatgga gaaaatccac
     8821 aacaagacgc tgatttctat caaaaagcct tgccaacaga ctactcaatt gcttctgcta
     8881 aacagctcaa cggaaaagaa ttgtccttca ataatatccg tgatgccgat gcagcaatcc
     8941 gtattatccg cgatttcaaa gaccgtccga cagttattgc cctcaaacac atgaacccat
     9001 gtggtatcgg acaggctgat gacattgaga ctgcttggga ttacgcttat gaagctgacc
     9061 cagtttcaat ctttggtgga attgtcgtcc ttaaccgtga agttgacgca gcgacagcca
     9121 agaagatgca ccctatcttc ttggaaatca tcatcgcacc atcatactca gaagaagcgc
     9181 tagctattct cacaaataaa aagaaaaact tgcgcatcct tgagttgccg tttgatgccc
     9241 aagctgccag cgaagtggaa gctgagtaca ctggcgtagt tggtggactt ttggtgcaaa
     9301 accaagacgt tgtagctgaa aatccatctg actggcaagt ggtgacagac cgccaaccaa
     9361 cagaacaaga ggcgactgcc cttgagtttg cctggaaggc tatcaagtac gttaagtcta
     9421 acgggattat tattactaac gatcacatga cgcttggact cggtgcaggt caaaccaacc
     9481 gtgtcggctc agtcaagatt gctatcgagc aggctaaaga ccaccttgac ggtgccgttc
     9541 tagcatcaga tgccttcttc ccatttgcgg acaacattga agaagtcgct gccgcagggg
     9601 tcaaagcaat catccagcca ggcggctcag ttcgtgacca agactctatt gacgccgcaa
     9661 acaaacatgg cttgaccatg atcttcacag gtgtgagaca ttttaggcat taataaa
//
LOCUS       DAUELZ010000062         9685 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.063, whole genome shotgun sequence.
ACCESSION   DAUELZ010000062 DAUELZ010000000
VERSION     DAUELZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9685
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.063"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(971..1114)
                     /locus_tag="VHI09_001172"
     CDS             complement(971..1114)
                     /locus_tag="VHI09_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEQ6121053.1"
                     /translation="MSGGGAYVCKSQKPKERRRQGLSVYETLTLMIAFGTLIVAIMNN
                     KNK"
     gene            1317..2462
                     /locus_tag="VHI09_001173"
     CDS             1317..2462
                     /locus_tag="VHI09_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ6121054.1"
                     /translation="MKITEHKKKNGTIVYRASIYLGIDQMTGKRVKTSITGRTRKEVN
                     QKAKHAQLDFLSNGSTIKRKVVIKTFKELSHLWLETYKLTVKPQTYDATVTRLNRHIM
                     PTLGNMKVDKITASDIQMLINRLSKYYVNYTAVRSVIRKVLQQGVLLGLIDYNSARDI
                     ILPRKQPNAKKKVKFIDPSDLKSFLEHLETSQHKRYNLYFDAVLYQLLLSTGLRIGEA
                     CALEWGDIDLENGTIAINKTYNKNLKFLSTAKTQSGNRVISVDKKTLRSLKLYQMRQR
                     QLFNEVGARVSEVVFATPTRKYFNASVRQSALDTRCKEAGIERFTFHAFRHTHASLLL
                     NAGISYKELQYRLGHANISMTLDTYGHLSKDKEKEAVLYYEKAMNNL"
     gene            2557..4539
                     /gene="mutL"
                     /locus_tag="VHI09_001174"
     CDS             2557..4539
                     /gene="mutL"
                     /locus_tag="VHI09_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEQ6121055.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLAISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQSRGLYYDPQKNDFFVKESAVSEKIPETDFYSGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNIFTETDHPNLDLKNRQKLSQMLTRLE
                     NEEKSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGEVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            4550..5770
                     /locus_tag="VHI09_001175"
     CDS             4550..5770
                     /locus_tag="VHI09_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ6121056.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFSTILANLPNAAIPW
                     LTFAGILLVEIASSFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYYFDETRPETHAFDHGKGLLDSFRNYRQVFHDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQFYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            5772..6368
                     /gene="ruvA"
                     /locus_tag="VHI09_001176"
     CDS             5772..6368
                     /gene="ruvA"
                     /locus_tag="VHI09_001176"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEQ6121057.1"
                     /translation="MYDYIKGQLTKITAKYIVVEANGLGYMINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGHTKARSNKAGNTQLDEAIEAL
                     LALGYKAKELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            6378..6938
                     /locus_tag="VHI09_001177"
     CDS             6378..6938
                     /locus_tag="VHI09_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEQ6121058.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLHDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVATFTSEELADALENPSIIRHKLKLAATVNNAIA
                     VQKIQEEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            7007..8278
                     /locus_tag="VHI09_001178"
     CDS             7007..8278
                     /locus_tag="VHI09_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEQ6121059.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQTLAKYLRRDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPI
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKSITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVCYIATLHAFNMVRKTLLKLENLL"
     gene            8367..9503
                     /gene="recA"
                     /locus_tag="VHI09_001179"
     CDS             8367..9503
                     /gene="recA"
                     /locus_tag="VHI09_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEQ6121060.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPQLFD
                     EIDRKVRVKFGLLEESEEESAMAVASEETDDLALDLDNGIEIED"
BASE COUNT         2976 a         1679 c         2046 g         2984 t
ORIGIN      
        1 agataaagaa gtgtttcttg aattttgtca aaaagatttt gaaatgtctg gcttgctttc
       61 tcttccgttc taattgtaag aaaacatcag gagttattag cctcaacgtg caacatgccc
      121 caaccaatac acaacgccaa ttgtatttat atttgttcac ttccccttta aggactcgca
      181 tttcttgcat tttgaccttt aaggaggccg aatactatat actaaaaatg ccaactgatt
      241 ttagaagctg tcacaacgga aaaaataaat taataaacga ccgatatatc aagttttttc
      301 aagttcttta agtgaatttc ccgagcgttt tagggctatt gaaataggtt gacgtattat
      361 gtcagtacgt gccaacattt tccagcattc cgaaatgcga ccatgtgttc gtgtttggga
      421 gtagtaacct tctattctct aagttcgctc gaagttcagc attgtgcgcg tggaataaaa
      481 ttagctacct tactgtaacc ttaccgttac cgctcatttt atgacctgtt caattttcat
      541 gtctaaatgc caaatttatt atctttacct acgcgcaaat accttgatac gcctttaatt
      601 ttctttaatt acaagaccct caaaacttga caaaaattga ggtataatct gaacttttct
      661 gaactttttg tcggcggtaa ttaaaaaaac tgtctgcgcg tggtcagtgg ttgcataaag
      721 cattataaat tcttgcataa aattattttt attcattttg gtaacactaa ggaatgttaa
      781 gagcgctaaa gactatactt tctaactata ccctttgact tctttatcaa acaaagctat
      841 aatggacata gaaaaaggag attgcgcaaa caatctcctg tggtagcacc gtttaagacg
      901 gcagccttac cgtatttgtt tatattttct ataaaccgtc cacgattggc taaagtgtgg
      961 gacggttttt ctatttgttc ttgttattca tgatagctac tatcagagta ccaaaggcaa
     1021 tcatcaaagt aagcgtttca taaactgaca aaccttgtcg tctcctttct tttggtttct
     1081 gtgacttaca tacataagca ccacctccag acataaggct acgactacct tgacctagtt
     1141 tttagacatg caaaacttct tctgaaactc tctatacctt acaaaaccaa acaaaaaaga
     1201 ccccgcaagt tttccacgct cgcaaggtct taaaaagact aatattatac catggtatga
     1261 actaatctaa aaccctttta ataatagctt gcctgctgat ggaaaggttt ataatcatga
     1321 aaataacaga acataagaag aaaaatggta caatcgttta tcgtgctagt atttatctag
     1381 gcattgacca aatgacaggt aagagagtaa aaacaagcat cacaggaaga acaagaaaag
     1441 aagttaatca aaaagccaag cacgcgcagc ttgacttcct atctaatgga tctacaatta
     1501 aaagaaaagt tgtgattaaa acatttaaag aacttagtca tttatggctt gaaacctata
     1561 agttaacagt aaagcctcaa acttatgatg ctactgttac tagacttaat cgacatatta
     1621 tgccaactct gggcaatatg aaggttgata agataaccgc tagtgatatt caaatgctga
     1681 ttaatagatt atctaaatat tatgtcaatt atactgcggt acgttcagtc atccgaaaag
     1741 ttctccaaca aggagtattg ctagggttaa tagattataa ctcagcaaga gatattatcc
     1801 ttccaaggaa gcagccaaac gctaagaaaa aagttaagtt tattgatccg tctgatttga
     1861 aatctttttt agaacattta gaaactagtc aacacaaacg ctataacctt tactttgatg
     1921 cagttctcta ccaactttta ttatccactg gcttgaggat aggcgaagcc tgtgcattag
     1981 agtggggaga tattgaccta gaaaatggta caatagccat taataagact tacaataaaa
     2041 atttgaagtt tttgagtaca gctaaaaccc agtcaggcaa tagagtgatt agtgttgata
     2101 aaaagaccct tagaagccta aagctctatc aaatgagaca gcgacaatta tttaatgagg
     2161 ttggtgcgcg tgtgtcggag gtagtgtttg ccacaccaac acgaaagtat tttaatgctt
     2221 cggttagaca aagcgcttta gatactaggt gtaaggaagc agggattgaa cgctttacct
     2281 ttcacgcttt tagacacact cacgctagtt tattgctgaa cgctggtatt agttataagg
     2341 aacttcagta ccgtctagga catgcgaata tcagcatgac tttggatacc tatggccatc
     2401 tttctaagga caaagaaaaa gaagctgttt tatattatga aaaggctatg aataatttat
     2461 aagtccacaa aaaagtccac aaattaatat tttgagaggt gtaaacctaa tgaaaccttg
     2521 ttatatcaac gtttagaaag tgttaaaaac aaaattatga caaacattat tgaattaccg
     2581 gaagttctcg ccaaccaaat tgcagctggt gaagttgtag aaaggccagc gagtgttgtc
     2641 aaggaattgg ttgagaatgc tattgatgct aaaagtagcc agattaccgt tgaaattgaa
     2701 gagtctggcc ttaagatgat acaggttaca gacaacggtg agggaatgtc tcatgaagat
     2761 ttacctttaa gtctgcgtcg ccacgctact agtaaaatta agagtcagag tgatttgttt
     2821 agaattagaa cacttggctt ccgtggagag gctttaccgt ctgttgcctc tatcagtaaa
     2881 atcacgataa aaacagcaac aaaagaagtc actcacggtt ctcttcttat agctactggt
     2941 ggggaaattg agacacttga agcgatctca actcctactg gaaccaaaat taaggttgaa
     3001 aacctttttt acaacacgcc tgctcgtctc aaatacatga aaagtttaca ggcagaatta
     3061 gctcacattg tagatgtggt caaccggttg agtttggcac atccagaagt tgctttcaca
     3121 ctgattagtg atggtcgcca attaactcag acatcaggaa ctggcgattt acgccaagca
     3181 attgcaggga tttatggttt aaatactacc aaaaaaatgc tggctatctc taatgctgat
     3241 ttggattttg aagtttccgg ctatgttagc ttgccagagt tgacacgcgc caaccgtaat
     3301 tacatgacga ttttggttaa tgggcgttac atcaagaatt tcttgcttaa tcgagcaatt
     3361 cttgatggtt acggttctaa gctcatggtt ggacgtttcc caattgttgt gattgatatt
     3421 cagattgatc cctatttggc cgatgtcaat gttcatccca caaaacaaga ggttcgtatt
     3481 tcaaaagagc gtgagttgat ggctttaatt agtacggcga tttctgaaag tcttaaggaa
     3541 caagatttga ttccagatgc tctggaaaac ttagccaaat caagcacacg acatttctct
     3601 aaaccagagc aaacacaact tcctttacag tctaggggac tttattatga tcctcaaaag
     3661 aatgactttt ttgtcaaaga gtcggctgtc tcggaaaaaa tacctgaaac tgatttttat
     3721 tccggcgctg ttgacaacag tgtaaaggtt gaaaaagcag agctgttacc ccactcagaa
     3781 gaagttatag gaccttcttc ggttaaacac gcaagtcgtc cccagaatat ctttaccgag
     3841 actgatcatc ctaatcttga cttaaaaaac agacaaaagt tatctcaaat gctaacccgt
     3901 ttggaaaatg aagaaaaatc agtatttcct gaattggatt attttggtca gatgcacgga
     3961 acttatctct ttgctcaggg aaaagatggt ttatttataa ttgaccaaca cgctgcccag
     4021 gagcgggtta aatacgaata ttatcgtgat aagataggtg aggttgacag tagtttacag
     4081 caattgttgg tgccctattt gtttgagttt tccggttctg atttcattaa cttacaggag
     4141 aaaatggcac tcttaaatga agttggtatc ttcttagaag tttatgggca caatactttc
     4201 attttgaggg agcatcctat ctggatgaaa gaagaagaga ttgcatctgg tgtctatgaa
     4261 atgtgtgaca tgttacttct aaccaatgag gtatctatta aaacttaccg agcagagtta
     4321 gctattatga tgagttgtaa acgatctatc aaggcaaatc atagcttgga tgattattca
     4381 gcaagaaatc tgctactgca attggcccaa tgtcaaaatc cttataactg ccctcatggt
     4441 agacccgtat tgattaattt tagtaaggca gatatggaaa aaatgttccg tcgaattcaa
     4501 gaaaatcaca ctagcctgcg agagctagga aaatattaga gaggatgaga tgcaagagtt
     4561 tttaaacctt cctaagcaga ttcagctgag gcaactggta cgctttgtga ccattacctt
     4621 aggcagtagt atctttccct ttatggccat gtattatacg acttactttg gtacgttttg
     4681 gacaggcctc ttaatgatga ttaccagttt gatgggattt gttggaactt tatacggtgg
     4741 gcatttgtca gatgctcttg gtcgcaaaaa agtcattatg attgggtcag taggaacaac
     4801 gctaggctgg ttttcgacta ttttagctaa tttgcctaat gcggctattc cttggttaac
     4861 ctttgcgggt attttattgg tagagattgc ttctagtttt tatggtcctg cctatgaagc
     4921 tatgttgatt gatttgactg atgagagtaa tcgtcgattt gtttacacca tcaattattg
     4981 gtttatcaat attgccgtca tgtttggtgc agggctatct gggctttttt atgatcatca
     5041 ttttttagcc ttgttagtag ctttattact cgtcaatgta ctttgttttg gcgttgctta
     5101 ctactatttt gatgagacta gaccagagac acatgctttt gatcatggaa aaggcttgct
     5161 ggatagtttt cgaaattatc gtcaggtgtt tcatgatcgt gcctttgtct tgtttacctt
     5221 aggtgccatc ttttctggta gtatctggat gcagatggat aactatgtgc cagtccattt
     5281 gaaactgtat tttcagccaa cggctgtgtt aggtttccaa gtaactagtt ctaaaatgtt
     5341 atcattaatg gttttaacta atacattgct gattgtcctt ttcatgacag tagtaaataa
     5401 attaacggaa aaatggaaac tattacctca gcttgtggtt ggttctttac tatttactct
     5461 agggatgctc ttggcattta cctttacgca gttctatgct atctggttat cagttgtttt
     5521 gttaactttt ggggaaatga taaatgttcc tgctagtcaa gtcctacgtg ctgatatgat
     5581 ggatcattcc caaataggat cttatacagg ttttgtgtca atggcacaac ccctaggtgc
     5641 tattttggct agtctcctag tatctgtcag ccattttaca ggtcctttag gcgtgcaatg
     5701 cttatttgta gtcattgctt tgctagggat ttattttacg gttgtttctg caaaaatgaa
     5761 aaaggtgtaa tatgtacgat tatattaaag gtcaattgac caaaattacg gcaaaataca
     5821 ttgtcgttga agctaatgga ttgggctaca tgattaatgt tgccaatcct tatagcttta
     5881 cagatagtgt caaccaattg gtaaccattt atctgcatca agtgattcgt gaggatgctc
     5941 acctgttgtt tggttttcac acggaagatg aaaaagatgt ttttctgaaa ttaatttctg
     6001 tatcaggtat tggtccgaca acagctcttg ctattgtggc agttgatgat aatgagggac
     6061 ttgtgaatgc cattgataac agtgacatta agtacctaat gaaatttcct aaaattggta
     6121 aaaaaacagc gcagcaaatg gttcttgact tagctggcaa atttgtggag gctccacaag
     6181 agactggtca taccaaagcg cgcagcaata aagcaggcaa tactcaactg gacgaagcga
     6241 ttgaagccct ccttgccctc ggttataaag caaaagagct gaaaaaaatt cgtgccttct
     6301 ttgaggggac ctctgagacg gcagagcaat acatcaaatc agcattgaaa ctgttaatga
     6361 aagggtaggt gtttcacatg aaacgttgct catgggttcc taaggataac cagctttact
     6421 gtgattacca cgatttagaa tggggtcaac ccctacatga tgatagagat ttttttgaat
     6481 tactctgcct tgaaagttac cagtcaggct tatcctggct gactgttttg aaaaaacgcc
     6541 aagctttcag aactgttttt catcattacg acattgcatc ggttgcaacc tttacctcgg
     6601 aggaactggc tgacgcttta gagaatccat ccatcattcg ccacaagcta aagctagcag
     6661 caactgtcaa caatgctatc gcagtacaga aaattcaaga agagtttggt agtttttcaa
     6721 cttatctctg gaattttgtt gggggaaagc caattaacaa ccttgtcaat caagagaatc
     6781 ttgttcctgc tcaaacagaa ttatctattc gcttagctaa ggatttaaag aaaagaggat
     6841 ttaaattttt agggcctaca acggtttatt cttttatgca agcatcgggt ctggtaaatg
     6901 accatgaaga ggcttgtgtt ttcaatagaa tcttctaatt cttcgtgaat tggaagattt
     6961 tttgataaaa tgagatcaga ttacgaatag ataagtaggt ggggaaatga aagctgaact
     7021 gattgcagta ggtaccgaaa ttttgactgg tcaaattgtg aataccaatg ctcaatttct
     7081 gtcggaaaaa atggcagagc taggtattga tgtctatttt caaacggctg ttggggacaa
     7141 cgaggagcgt ttactttcag tgattacaac tgctagtcag cggagtgatt tggtcatttt
     7201 atgcggtggc cttggtccaa cgaaagatga tttaaccaaa caaactttag caaagtacct
     7261 taggagagac ttggtttatg atgagcaagc ttgtcagaaa ctagatgact tttttgctaa
     7321 gcgcaagcct tcatcacgga caccaaataa tgagcgacag gcacaagtga ttgaaggatc
     7381 aatccctttg ccaaataaaa ctggtcttgc ggttggtggg ttcattacag tcgatggtat
     7441 tagttatgtt gtcttaccgg gtcctccaag tgaattgaag ccgatagtaa atgaagaatt
     7501 ggtaccactt ctgtcaaaac aatacagtac attgtattca aaggtactac gcttttttgg
     7561 tattggggaa agtcagttgg taacagtctt gtcagatttt attgagaatc aaactgatcc
     7621 aaccattgct ccgtatgcta agactggcga agtgactctt cgcttatcaa caaaaactga
     7681 aaaccaagct ctggcagata aaaagttagg tcagctagaa gcgcagctac tatcccgaaa
     7741 aactcttgaa ggtcaaccct tagctgatgt cttttatggc tatggggagg ataattcctt
     7801 agcgcgtgag acatttgagc tcttagtaaa atatgataag tcaattacag cagcagaaag
     7861 tctaaccgcg ggattatttc agtcaacttt ggcgagtttt ccaggagctt ctcaagtatt
     7921 caatggaggc tttgtgactt atagcatgga agaaaaagcg aaaatgctag gccttccttt
     7981 agaggagttg aaatcgcatg gcgttgttag tgcttatacg gccgagggga tggcggagca
     8041 agcaaggtta ttgactggtg ctgatattgg ggtaagttta acaggtgttg ccggaccaga
     8101 tatgttggag gaacagcctg caggtacagt tttcattggt cttgccactc aaaataaggt
     8161 agaatcaata aaggttttga ttagcgggcg aagtcgtttg gatgtgtgct atatcgctac
     8221 tttacatgcc tttaatatgg tccgtaaaac tttattaaaa cttgagaatt tgctataatg
     8281 ttatttagta gctatagtag gatatggttt acaattatgt agattaatcc tgctgacgat
     8341 ggtttgttaa aaaagaggag aaagtattgg caaaaaaatt aaaaaagaat gaagaaatca
     8401 ctaagaagtt tggtgacgaa cgtcgcaaag ctcttgatga tgctttgaaa aatattgaaa
     8461 aagatttcgg taagggcgca gtgatgcgat taggagaacg tgcagagcaa aaagttcagg
     8521 ttatgagttc aggaagtcta gctcttgata ttgcgcttgg agctggtggt tatcctaaag
     8581 gacgtatcat cgaaatctat ggtccagagt cttccggtaa aacgactgtg gctttacatg
     8641 ctgtagcaca agctcaaaaa gaaggtggaa tcgcagcctt tatcgatgcc gagcatgcgc
     8701 ttgatccagc ttatgctgct gcgcttgggg ttaatattga tgaactcctc ttgtctcaac
     8761 cagattctgg agaacaagga cttgaaattg caggtaaatt gattgattct ggtgcggttg
     8821 atctggttgt tgtcgattca gtagcagctt tagtgccacg tgctgaaatt gatggtgata
     8881 ttggcgatag ccatgtcgga ttgcaagcac gtatgatgag tcaggccatg cgtaaattat
     8941 cagcttctat taataaaaca aaaactatcg ctatttttat taaccaattg cgtgaaaaag
     9001 ttggtgtgat gtttggaaat cctgaaacaa caccaggtgg tcgagctttg aaattctatg
     9061 cttctgttcg gctggatgtg cgtggaacaa ctcaaattaa aggaactggt gaccaaaagg
     9121 atagcagcat tggtaaggag accaaaatca aggttgttaa aaacaaggtc gctccgccat
     9181 ttaaggtagc agaagttgaa atcatgtatg gggaaggtat ttctcgtaca ggggagcttg
     9241 tgaaaattgc ttctgatttg gacattatcc aaaaagcagg tgcttggttc tcttataatg
     9301 gtgagaagat tggccaaggt tctgaaaatg ctaagcgtta tttggccgat catccacaat
     9361 tgtttgatga aatcgaccgt aaagtacgtg ttaaatttgg tttgcttgaa gaaagcgaag
     9421 aagaatctgc tatggcagta gcatcagaag aaaccgatga tcttgcttta gatttagata
     9481 atggtattga aattgaagat taaaaagagc ccttcagggc tttttaaaat gtatttttaa
     9541 aggttagtag gactactgtt cataggtgtg tgggtgaaaa gaaactatca tttaaaaaga
     9601 tgtgagagag cagaacttag gttaactgat ataaaattaa aataactaat aattcaaggg
     9661 aatacaggtg aaaaacctta tttta
//
LOCUS       DAUELZ010000063         9483 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.064, whole genome shotgun sequence.
ACCESSION   DAUELZ010000063 DAUELZ010000000
VERSION     DAUELZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.064"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            104..754
                     /locus_tag="VHI09_001180"
     CDS             104..754
                     /locus_tag="VHI09_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ6121061.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNAEKDYILNAKPNGYRSYHLILELETHFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            757..1425
                     /locus_tag="VHI09_001181"
     CDS             757..1425
                     /locus_tag="VHI09_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ6121062.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYC
                     LLEK"
     gene            1434..2666
                     /locus_tag="VHI09_001182"
     CDS             1434..2666
                     /locus_tag="VHI09_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ6121063.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPDTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSMMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
     gene            2960..3838
                     /gene="mvk"
                     /locus_tag="VHI09_001183"
     CDS             2960..3838
                     /gene="mvk"
                     /locus_tag="VHI09_001183"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEQ6121064.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLIIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            3820..4764
                     /gene="mvaD"
                     /locus_tag="VHI09_001184"
     CDS             3820..4764
                     /gene="mvaD"
                     /locus_tag="VHI09_001184"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEQ6121065.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSISFLPDTATSDQFYINGVLQNDEEHTKISAIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFNTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDEWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            4757..5764
                     /locus_tag="VHI09_001185"
     CDS             4757..5764
                     /locus_tag="VHI09_001185"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEQ6121066.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFAHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHPAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQDARDTLISKWQEADIALLYQERWGEN
                     D"
     gene            5757..6746
                     /locus_tag="VHI09_001186"
     CDS             5757..6746
                     /locus_tag="VHI09_001186"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEQ6121067.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQQVN"
     gene            complement(6976..8253)
                     /locus_tag="VHI09_001187"
     CDS             complement(6976..8253)
                     /locus_tag="VHI09_001187"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEQ6121068.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDRDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQAGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(8240..9415)
                     /locus_tag="VHI09_001188"
     CDS             complement(8240..9415)
                     /locus_tag="VHI09_001188"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEQ6121069.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRAKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNKDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAVCFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHQRIYHTNDKN"
BASE COUNT         2980 a         1716 c         1926 g         2861 t
ORIGIN      
        1 aacaaacgat acagatcatt tgcagttata tagttagaaa taaagataac cgatcattgc
       61 agagatttaa gcatttgttg agaaaatttg ttataataag cgtatgaaag aaagactaga
      121 acattattct accagctcta tatatagcgg atttgaagtg tacttgccgt tggtactaca
      181 gacaataaca gatgtgatta ttgctgagaa tatcaaaagc aaaaaagaaa cggggtttaa
      241 actatacgaa cactttacat cccgtattaa atcggaagct tcgatgattg aaaaatgtca
      301 acgcaaacag cttcctctaa caagcaaatc tgcgttaaaa atcattaaag atagtatcgg
      361 tattcgcatt atttgtggtt ttattgatga tatttatcgg atggtagact tgctcaaaag
      421 cattcctggt atgtcagtta acgctgaaaa agattatatt ttaaatgcca agcctaatgg
      481 ctatagatca tatcatctca ttttggaatt agaaacacat tttccagata ttttagggga
      541 aaaaaaagga tgttatttta tagaagtgca gttgcgaacc attgctcaag actcttgggc
      601 tagcttggaa catcaaatga agtacaaaca tcaagttgct aatgcagaaa tgattacaag
      661 agagttaaag cgttgtgcag acgagttggc atcttgtgat gttaccatgc aaaccattcg
      721 tcaattgatt caagaaacga cggaggaaga gtaaccatga aaattttgtt agctgaagac
      781 gaatggcaga tgtctaatgt tttgacaact gccatgactc atcaaggtta tgacgttgat
      841 gtggttttta acggtcaaga ggcaattgat aaagccaaag acaatgcata tgatattatg
      901 attttagata tcatgatgcc gattaagtct ggcattgagg ctttaaagga gattcgcgct
      961 tcaggaaatt gctcacatat tattatgctg acggctatgg cagaaattaa cgaccgtgtt
     1021 accggactag atgcgggagc tgatgattat ctgactaaac ctttctcatt aaaagaactt
     1081 ttagctagat taaggtcgat ggaaagacga gtagaaagtt ttactcctca ggtattacaa
     1141 tttgctggtg tcacattaaa tattaatgag caagaattga gtgccggtaa tgctatacga
     1201 ttagcttcta aagaaggtaa attaatggcc tttttaatgt taaatcaagg aaagtacttg
     1261 gatactaaaa cactttacca acatgtttgg tcagaccaag aagattatga tacttctatt
     1321 gtttgggttt atatttctta tcttcgtcaa aaattattag ctattcaagc aaatgtcatt
     1381 attgcaggtg ataaagatac tagctattgt ttattagaaa aataggaggt gacatgttta
     1441 atcgtatccg tattcgtttt atcatgattg cttctattgc catttttatt attttaagtt
     1501 caattgttgg tattattaac acggcacgct gttaccaaag tcaacaagaa attaaccgta
     1561 ttttacattt aatttcctca aataaaggta aattacctga cactacggaa agctctaaac
     1621 gacttggaac gaaactgtct gaagatagcc taagccagtt tcggtattat agtgtcattt
     1681 ttaatgctaa tggacatcta ctttcttcta atactgctaa tatttcagct ttagatagag
     1741 aagaagccca atattttgct agactttttg ctaagtctgg ggaagaaaaa ggcagttacc
     1801 gtcaccaaga tagtgtttat tcgtacttga ttactcagct gccaaatgaa gaaaaactgg
     1861 ttgtaatttt agatactacc ttttattttc gtagtgtagg agatttatta gctgtttcaa
     1921 tgatgttggc ctttggagga tttatctttt ttgttgtttt agtgagtctt ttttccggta
     1981 tggtcatcaa accttttgtc caaaattatg agaagcagcg tcgctttatt actaatgcag
     2041 gccacgagtt aaaaacacca ctagccatta tctcagccaa taatgaattg gtcgaattaa
     2101 tgactggaga atcagaatgg accaagagta caagtgatca ggtgaagcga ttgacaggct
     2161 tgattaatca aatgattact ttggctcgtt tggaggaaca gccagatgtg gtattacata
     2221 tggtagactt ttcagctatt gctcaagatg cagctgaaga ttttaagagt ctggttttga
     2281 aagatggtaa acgttttgat ttgacgattc agccgaatat tatgattaaa gcggaggaaa
     2341 agtcattatt tgaacttgtg acaattttag ttgataatgc caataaatat tgtgatccta
     2401 aaggcttggt caaggtatct ttaaccacta ttggaagaag acgaaaacga gcgaaattag
     2461 aagtttctaa tacctatttg gaaggaaaat ccattgatta cagccgcttt tttgaacgct
     2521 tttatcgcga agacgaatcc cataatagca aagaaaaagg ttatggaatt ggtttatcta
     2581 tggcagaaag tatggttaag ttatttaaag gaacgataac tgtaaattat aaaaacgatg
     2641 ctattgtttt tacagtggtg atttgactta aacatttttc agtaccttta gctagtaata
     2701 atgtctaggt aaaggtaata aacgatgctt aaccagccca tgaggctggt ttttaacgtt
     2761 taatttagta gaagagtgac taaagaaggt agtttgaaat aagaaaattt ttgttatgat
     2821 cctaaaaaga gtagatagtc tttattgtta atttcagtga agaaatgatt ttaaaaagat
     2881 atatcatcgc attttctaat catttagtgt aaaatagtaa ggtaaaagtt tagaagaatt
     2941 agtaatgaaa agataaaaaa tgaacgaaaa cattggatat ggtaaggcac acagtaagat
     3001 cattttgata ggtgagcatg ctgttgtgta tggctaccca gctattgctt tgcctttaac
     3061 agatattgag gtggtttgtc atatttttcc agctgataaa ccattggtct ttgattttta
     3121 tgatactcta tcaacagcca tttatgctgc cttggattat ttgcagcgac tacaagaacc
     3181 aattgcttat gagattgttt cacaagtgcc acaaaagcgt ggtatgggat cttcggcagc
     3241 cgtttctatt gcagctatta gagccgtttt ttcttattgt caagaacccc tctcggatga
     3301 tttgttggaa attttagtca acaaggcaga aattattgcc cacaccaatc ctagtggttt
     3361 agatgccaag acatgcctta gtgaccatgc cattaagttt atccgaaaca ttggctttga
     3421 aactatcgaa atcgccttaa atggttatct tatcattgca gatacaggga ttcacggtca
     3481 tacacgtgag gccgtcaata aggtagcaca gtttgaggaa acgaatttgc cctatctggc
     3541 taaactagga gctttgacac aagcccttga aagagcgatt aaccaaaaaa ataaggttgc
     3601 tatcggtcag ctcatgacac aagcgcactc tgctttaaag gccattggcg ttagcatcag
     3661 taaggctgac caacttgtgg aggctgccct aagagcaggt gctttaggcg ctaagatgac
     3721 aggcggtggc ttgggtggct gtatgattgc cctagcagat accaaagaca tggccgaaaa
     3781 aatcagtcac agattaaaag aagaaggagc cgtaaacacg tggatccaaa tgttataacc
     3841 gtaacatcct atgctaacat tgctattatc aaatattggg gaaaagagaa tcaagctaag
     3901 atgattcctt cgacctctag catttctttg actttggaaa acatgttcac cacaaccagc
     3961 atttccttct taccagatac tgcaaccagt gatcagtttt acattaacgg tgtcttgcaa
     4021 aatgacgaag aacataccaa aatttctgct atcattgacc aatttcgcca acctggtcag
     4081 gcttttgtaa agatggaaac tcaaaataat atgccaacag ctgcaggttt atcatcaagt
     4141 tccagtggct tatcagcctt ggttaaagcc tgtgaccaac tatttaacac ccagctagat
     4201 cagaaagctt tagctcaaaa ggctaagttc gcctcaggat catcttctcg ttcttttttt
     4261 ggcccagtcg ctgcttggga caaagatagt ggtgctattt acaaggttga gactgacttg
     4321 aaaatggcca tgattatgct ggtcttaaat gctgcgaaaa agccaatttc tagccgagag
     4381 ggcatgaagt tatgccgcga tacctcaact acatttgatg agtgggtaga acaatcggca
     4441 atcgattacc aacatatgct tacctatctc aaaactaata attttgagaa agttggtcag
     4501 ttaacagaag ctaatgcctt ggccatgcat gccacgacaa agacagccaa tcctcctttt
     4561 tcttacctaa caaaagagtc ttaccaggct atggaggctg tgaaagaact gcgtcaagaa
     4621 ggttttgctt gttattttac catggacgca ggtccaaatg tgaaggtcct atgtttagaa
     4681 aaagacttgg ctcaactggc agaacgactt ggcaagaact accgtattat cgtttcaaaa
     4741 acaaaggatt taccagatgt ctaattattg tgtgcaaaca ggtgggaaac tatacctcac
     4801 aggcgaatat gctatcttaa caccaggaca aaaagcctta attcacttta ttccactgat
     4861 gatgacagca gaaattagcc cagcagccca tattcaatta gcttcagata tgtttgccca
     4921 taaagcgggc atgacacccg atgcctctta tgcactgatt caagcaacgg ttaaaacctt
     4981 tgctgactat ctaggacagt cgattgacca actggagcca ttttccctaa tcataacagg
     5041 aaaaatggag cgcgatggca aaaaatttgg cattggttca agtggtagcg tcaccctctt
     5101 aaccttaaag gccttatcgg cctattatca gatcacttta accccagagt tactctttaa
     5161 actagcggct tataccttgc ttaagcaagg agataatggg tctatggggg atattgcctg
     5221 tatcgcttac cagactttag tcgcctatac ctcctttgac cgagaacagg tcagtaactg
     5281 gctgcagacc atgcccttaa aaaaacttct cgtcaaagat tggggttacc atatccaagt
     5341 cattcaacca gccctgcctt gtgacttttt ggttggctgg actaaaatac ctgctatttc
     5401 aaggcagatg attcaacaag tgacagcgag cattacccca gctttcttaa gaacaagtta
     5461 ccagctaacg caatcagcta tggtagcttt gcaagaaggt cataaggaag aactcaagaa
     5521 aagtttagca ggagcaagtc atctcctaaa agagcttcat ccagctatct accatcctaa
     5581 gctagtaacc ttggtagctg cttgtcagaa gcaagatgct gttgctaaat cttcaggttc
     5641 tggtggtgga gattgtggca tcgcgcttgc ctttaatcag gatgctagag atacccttat
     5701 ttccaaatgg caagaagctg atatcgcatt actttatcaa gaaaggtggg gagagaatga
     5761 ctaaccgtaa agatgatcac atcaaatatg ctctcaagta ccaatcgcct tataatgctt
     5821 ttgatgacat agaactcata caccattcct tacctagcta tgatttgtct gatattgatc
     5881 tcagtactca ttttgctggg caagacttcg actttccctt ttacatcaat gccatgacag
     5941 gaggaagtca aaaaggcaaa gctgtcaatg aaaaattggc caaagtagca gcagcaacag
     6001 ggattgtcat ggtgacaggg tcttatagcg ctgctttaaa aaatcctaac gacgattcct
     6061 atcgtttaca tgaggtggca gataacttga aactagccac gaatattggt ctagataaac
     6121 ctgtggcgct aggacaacaa acggttcaag aaatgcagcc cctcttttta caggttcatg
     6181 tgaatgtgat gcaagagttg ctgatgccag agggtgagcg cgtctttcat acctggaaaa
     6241 aacacctcgc tgaatacgct agtcaaatac cagttcctgt cattctcaaa gaagttggtt
     6301 ttggcatgga tgtcaatagt atcaagctag cacatgacct aggcattcaa acctttgata
     6361 tttcaggtag aggaggaact tcatttgctt acattgaaaa tcaaagaggg ggagaccgct
     6421 cttacttaaa cgattgggga caaaccactg ttcagtgctt actgaatgca caaggactga
     6481 tggaccaagt ggaaatctta gcttcgggtg gtgtcagaca ccccttggac atgattaagt
     6541 gttttgtctt aggagcacgt gcagtgggac tctcacgcac cgttttagaa ttggtcgaaa
     6601 aatacccaac cgagcgtgtg attgctatcg ttaatggctg gaaagaagaa ttaaaaatca
     6661 ttatgtgtgc tcttgactgt aaaactatta aagaattaaa gggagtcgac tacttactat
     6721 atggacgctt gcagcaggtc aattaggctt catcacctct acaatagtgt cacctactac
     6781 aaagatgata gagctttttt agaacataat ataaaatatt tataaatacc tcatcaaact
     6841 cagaatagag tagatgagta aatagagtat gttatggaga ctatttctaa gacttgtatg
     6901 tatagaaaca aagattaaac acccatgtaa accaattgtt tacatgggtg tttgccttat
     6961 attagttgtt taaatctaag actgtcttag ctgggataag taaaaatgtg ctttttctag
     7021 atttattggt ttatcagcta gtaaagcatt aactaaagga gcaatttcgt cttgggttgc
     7081 ccccgccaaa agagctaatg atttcgcatg tagtttcatg tgaccagctt ggatgccaga
     7141 tgaggtcaga gcttttagtg cagcaaagtt ttgagctaac cccacagatg caatcaattg
     7201 ggctaatgtt ttggcatcag gttgattaag aagatcatgt gcgatagaaa cagtagggtt
     7261 aagcccgata gatcccccct tactagcaat aggcataggg agggtcattt ggccaagcag
     7321 aacttgttta tcttggtcaa aatgccactg actaagtcct tgatagctac catttcttga
     7381 ggcataagca tgggcccctg cttcaatagc acgccagtca tttcctgtgg ctattaccac
     7441 tgcatcaata ccattaaaaa tacctttatt atgagtagta gcccggtaag gatctacttg
     7501 agctagtcgg ctggctagct ctattttttg agctaactgt agggattttg ttttatcgcg
     7561 atctaaaagg cgcacgggaa tctcacaact agtagtaaca agcgactctg ttgcgtaatt
     7621 agataaaatc gccattagac aatgcccctt agacagtctt gttaaatcag gaacaagagc
     7681 ttccatcata gtattgacca tatttgctcc catagcttct tgggtatcaa cagtcagata
     7741 gaaaatcaaa aattctcctt tttcttccaa atgaatggtt ctagcgcctc cacctctttt
     7801 aacaatggaa ggataagctt tatttgccaa tgcaattagc ttttttgttt tatgaaggat
     7861 ggcggcttta gcgttatcta tttggtcgat atcgtaaaga acaatttgac caatcatttg
     7921 tcggtttagg gtttgagctt taaaaccacc tgagcgtttg attagttttg ctgcgaaaga
     7981 tgctgcagca acaacagaag gttcttccgt gacaaaaggc atctgataag ttgaaccgtt
     8041 gacaagaaaa tcaggagcta tgctaaaggg caaagccaat cgtcctaaga cattttcagt
     8101 catttgatta gctgtttgga taggcaaaag aacgtctgtt ttgagttgat ttaagttttc
     8161 agcattaagt agtttaaatt tttcgataag ttggaggcgt tcttcgaatg ttttctttga
     8221 aaaaccactc cagttaagat tagtttttgt cattggtatg atagatcctt tgatgctcct
     8281 tgatggctgt tagagcaaat tttccagtta agtaaatgtc aaaattggca ttaccattat
     8341 catctaattg gacttgttcg tagaaaaggt cttcgtattt agaaacggaa agacgtgttc
     8401 gttgatctag taaagcttgt cgattagtca ttaacatttt atcgtaccca gcaactaact
     8461 gaccggaata aaattcactt acggcaccgg aaccatagct aaaaaaacca attttatctc
     8521 cagattgtaa tactttacta ttttcaagta aagataacaa tcctagataa agtgagccag
     8581 tataaatatt tccaatttgt ttgctataag taatagaagc ttgaaaggct tctattagtt
     8641 tttccctgtg ttcaggaggt actgtgttat ccataatgtt atttagacct tttagggcta
     8701 acttgggaaa tggaatatgg aaacaaactg cagcaaaatc agataactga aggttttctc
     8761 ttttctgata agcttgccac gtggtttcta agcaattgag atattgcttg gtagagtaaa
     8821 taccatcaac ataaggtgtg aaactatagt taggcctcca aaagtccata atatccctag
     8881 tttgagccac attatcttta tttagggcaa gaatacgagg gttagcagtt accaaaagag
     8941 caatactacc agagccttga gttgattcgc caggagatcc tacaccgtat ctagcaatat
     9001 cactagcaat aacaaggaca cgagaatctg gcttagaagc cacatgcagt ttagcatagt
     9061 ctagagcagc agttgcgcta tagcaggctt gttttacttc aaaggaacgt gcaaaaggct
     9121 ggataccaac taagtgatgt acatagatag ctgatgcctt tgactgatca gtactcgatt
     9181 cagttgccaa aataaccata tcaatcttag ctcgatcttc gtcagttaag atttgatctg
     9241 ctgcagaagc agctaaagta ataatgtctt cagtgattgg tgccacacta aaagattcaa
     9301 tgagtagccc ttgactaaat tttgctggat ccacttggcg tgcaagcgct aaatcttcca
     9361 atttcaagac atattgactg gttgcaaaac caatcttatc aattccaatt gtcatatttg
     9421 tccttctttt agttattttt atagtcttac ccattctatc acattttact gcaaaattag
     9481 act
//
LOCUS       DAUELZ010000064         9473 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.065, whole genome shotgun sequence.
ACCESSION   DAUELZ010000064 DAUELZ010000000
VERSION     DAUELZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.065"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            51..263
                     /locus_tag="VHI09_001189"
     CDS             51..263
                     /locus_tag="VHI09_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6121070.1"
                     /translation="MVTIAELRAKHNKMSQRELAKELGVTQTSISNWEKDQTKISGEY
                     LISLALFFDVSTDDILGINKQATQKF"
     gene            533..844
                     /locus_tag="VHI09_001190"
     CDS             533..844
                     /locus_tag="VHI09_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ6121071.1"
                     /translation="MPKAEITYRAVDVNETASHGDYKHLIQQWEGLTVATAKQWATEM
                     RDHPDFKQFVLNPTHRIVFIDYKGFKLFVQWKSRNRYKTKKETLPEMLENIKFEKRVG
                     V"
     gene            846..1031
                     /locus_tag="VHI09_001191"
     CDS             846..1031
                     /locus_tag="VHI09_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121072.1"
                     /translation="MNKLELFLLATTVILAVIARVQHEVIKKHNSPENKRKIFREVAL
                     KNSKGWSKKRARGGVVS"
     gene            1520..1906
                     /locus_tag="VHI09_001192"
     CDS             1520..1906
                     /locus_tag="VHI09_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ6121073.1"
                     /translation="MDEKKLFENFQLTFGRMISPFEIEDIQKWIHEDNMPIEVVNLAL
                     REAVENNKISWKYINKILVDWYKSGDTTVEKVRDRLQRFDDSKKQRSVTTSNVPSWSN
                     PDYKEPDLKEFALGSMDGIEDGSGDF"
     gene            1887..2121
                     /locus_tag="VHI09_001193"
                     /pseudo
     CDS             1887..2121
                     /locus_tag="VHI09_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2118..2258
                     /locus_tag="VHI09_001194"
     CDS             2118..2258
                     /locus_tag="VHI09_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121074.1"
                     /translation="MTKQHRETLIWYRASHQERERLLDFGLVDKARYVTLLRQLRKKY
                     AI"
     gene            2267..2473
                     /locus_tag="VHI09_001195"
     CDS             2267..2473
                     /locus_tag="VHI09_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121075.1"
                     /translation="MTPEQAEKAKIRAKQELETFSIYLDQAIDDLGGVLTSREVFLAA
                     GITYLGAGQTDIHAAVEGLCEQIQ"
     gene            2529..2858
                     /locus_tag="VHI09_001196"
     CDS             2529..2858
                     /locus_tag="VHI09_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121076.1"
                     /translation="MNIKEKIVVLRNTEDGSFLKSFKNKKDVLAYNVELTDSIQLASF
                     LPEEAYNIQKEKIDNLAETLGCDVVVIEASYDLKFIDGESVPELTKEQKVKSMVNGMF
                     EQVFGGE"
     gene            2882..3454
                     /gene="hylP"
                     /locus_tag="VHI09_001197"
                     /pseudo
     CDS             2882..3454
                     /gene="hylP"
                     /locus_tag="VHI09_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115223326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
     gene            3469..5250
                     /locus_tag="VHI09_001198"
     CDS             3469..5250
                     /locus_tag="VHI09_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121077.1"
                     /translation="MSRDPTYTINEHDLSFADGRFYVTFKADKSSETVRLNSSCLGNT
                     IIKKLQVEDDNTMHDFVKPKVTTQQAFGLAQQVKELDLQLKDPKSDLWGKIKFNNKAM
                     LVEYANKEMSSAIAQSAEQILLQVKSIDDERYSKFEQTLNGIKQTVKSESVESARTQL
                     ASMFDSRISGLDGKYSRLSQTIDSLSSRLDDGVGNYSTLSQKVSGIDLRVSNAANDVS
                     RLSQTAQGLQSQITNANQNYSSLSQTVGGLQTTVHDNQSNATSRINQLSDLISTKVTK
                     GDVETTIAQSYDKIAFAIRDKLPASKMTGSEIISAINLDRSGVKITGKNITLDGNSYI
                     SNAVIKDAHIANMDAGKINTGYLNASRIAAEAITGDKIKMDYAFFNKLTANEGYFRTL
                     FAKNIFTTSVQAVTTSASKITGGVLSATNGASRWDLNSANIDFNRDATINFNSKNNAL
                     VRKSGTHTAFVHFSNATPKGYRGSALYASIGITSSGDGIDSASSGRFCGVRFFRYAEG
                     LQHTAKVDQAEIYGDDIVFSDDFNIDRGFKMRPSLMPKMVDLNKMYQAILALGRCWLH
                     ANNTAWSWNFDTRSAIIAEYNAHINNL"
     gene            5259..5687
                     /locus_tag="VHI09_001199"
     CDS             5259..5687
                     /locus_tag="VHI09_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEQ6121078.1"
                     /translation="MDLTLKNKDLNTLYSVLDKIKITNMRANRGRAKLLAKVVDKFKE
                     YAKDEGDLIDLYAQKDKDGKFVIDEHKNIKLADPAKLDEFNGLLNELADEEIVIKGGE
                     YSKRFIDFLEYLAESEDEFTAQEIIIVDSILEQYEGSKGE"
     gene            5690..6322
                     /locus_tag="VHI09_001200"
     CDS             5690..6322
                     /locus_tag="VHI09_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121079.1"
                     /translation="MKTLTLSGKPYPIHEGGKVVKTEVRLIGDNGLFIPIELIGDQTA
                     KGADDLIKEGLDAFVREYVTKYAVAESVQKVEELSLAQKEIEQNAEQAKVTAEAAEKQ
                     AKSLELVIAKSQKMANLQAIHLLTSGSKVEPDIYKGLLELIEPAKQGEYQAYDVFTVV
                     DESHEEQAGEGNLVFVHVNEPFTYDKQTLKELEEEDKVTVIKYADLVKQD"
     gene            6334..6606
                     /locus_tag="VHI09_001201"
     CDS             6334..6606
                     /locus_tag="VHI09_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121080.1"
                     /translation="MATELIFGLGGFILAIVTTYNIFNAKSIKHATDITLLQSEVEHL
                     KIVTRQNARRLEEHDEQNKTLITMTEQIKNLNREVRELKDIMRGEA"
     gene            6603..6830
                     /locus_tag="VHI09_001202"
     CDS             6603..6830
                     /locus_tag="VHI09_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEQ6121081.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQLGIKLPSNIADIANTAV
                     TLLVLLGVVTDPTTKGLSDSEQALTYHEPKQ"
     gene            6949..8166
                     /locus_tag="VHI09_001203"
     CDS             6949..8166
                     /locus_tag="VHI09_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEQ6121082.1"
                     /translation="MVVDTEKAIAWMGLKKGRVSYSMDCRNGPDSYDCSSAICSALIY
                     AGASNPGWLLNTEYMHDWLVQNGFELIADNEDWDSQRADIAIWGFRGQSAGAGGHVVM
                     FIDADNIIHCNYANNNITIDNYNQTAAASGWMYSYVYRYTGAKAQPATNKSIDELVQE
                     VLSGKHGSGEQRKISLGTNYDAVQAKVNEMLKQPQVAEQSPAVKQDGDLLFNGAVLKK
                     TVLDKILAKCKEHDILPSYAITVLHFEGLWGQSAVGRTDNNWGGMTWTGQGNRPSGIT
                     VTQGTERPAVEGGHYMHYASVDDFLTDWFYLLRADGSYRVSGAKTFSEAVKGMFKVGG
                     ATYDYAASGFDSYIVGMAGRLKAIEQENGSLAKYDQQADIDVGQSDKIDVVIDSLEIT
                     INGVTYTATKKPI"
     gene            8302..9426
                     /locus_tag="VHI09_001204"
     CDS             8302..9426
                     /locus_tag="VHI09_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEQ6121083.1"
                     /translation="MALQGIKKLPVLLEDKQALSLYKKLAIVNKVLGKLDAVLESSII
                     NSSILSLLSYNESVQSTRIEGTQVTFHEIMETAKVGAKNWQQREVFNYKKAIDFGFHK
                     IKKGDVITTRLIKDLHRLLMSDEARGTTSNGGEFRKIQNFIGPDKDIENASYIPIPAN
                     EIGAFMTNLEFFINGEYHSSLECGRTQESINFNSDILLRIAVAHAQFESIHPFLDGNG
                     RLGRILIALMSVQEGLLKHPIFFVSEELEKERIRYYNALNATRGENPDWNLWLNLFLN
                     ASEQMAKNILKKIRNADEHAKKGLSVCVTQTQKTVWLATFSFPVSTAKQLAEATEFHP
                     ATVKKALDFLVGEGLLDKDNSVKRNVPYYNYDLIRAIQNY"
BASE COUNT         3328 a         1639 c         2042 g         2464 t
ORIGIN      
        1 tttaaaatca tatagaatgt aatcatcatc aagaaaggag cgactcagct atggtaacta
       61 ttgcagaatt aagagctaaa cataataaga tgtcacagcg tgaattggca aaagaattag
      121 gtgttactca aacatctatc agcaactggg aaaaagacca gacaaagatt tcaggtgagt
      181 atcttattag tctagctttg tttttcgatg tatctaccga tgacattctt gggataaaca
      241 agcaagcaac acaaaaattt taaacactat atgattttaa atcatatagt agaaaggaca
      301 cacatgaacg aaataacatt taccctatat tgcacaacat ctgaagaagc aatcacggaa
      361 gtaaagaaac taaaagaagc ccatccaaag gatagacttc gattcaatgt aaacattaag
      421 cctgagtttt actaatagaa ccaacttaat tagataataa aaagtccgac gggaatcgga
      481 ctccaaacaa aaaatattta cttaattata acacaagaga gaggatatta atatgccaaa
      541 agcagaaatt acttatagag cagttgatgt taatgaaaca gcttctcatg gtgattataa
      601 acacttgatt caacaatggg aggggttgac agttgctact gcaaagcagt gggcgacaga
      661 aatgcgtgat catccagact ttaaacagtt tgtattaaat ccaacgcata gaatcgtatt
      721 cattgattac aaaggattca aactgtttgt acagtggaaa tctcgcaatc gttacaaaac
      781 aaaaaaagag acactgccag aaatgcttga aaatattaaa tttgaaaaaa gagtaggagt
      841 ataacatgaa caaattagaa ttatttttgt tagcaacaac cgttatctta gcggtcattg
      901 ctagggtgca acacgaagtc attaaaaaac acaattcgcc agagaataag cggaaaattt
      961 ttagggaagt agctttaaaa aacagcaaag gatggagcaa gaagcgtgca agaggagggg
     1021 ttgtcagcta atgcagtaca tttttcaaga cattaacagg tagttacaca gctgttagca
     1081 atgaatttat tcaggacaaa gaacttttca ataaggaaaa aggactgctg ctaaccattt
     1141 tgagtaatgc agacgaatgg agagtctatc ctgaagaact cgctagacgt tgtaaggata
     1201 gcgaatctgc aatcagaagc cagttgaaag cgttagagaa tgccaagtat atcagaactt
     1261 atagaaaatc atttggcggg cgatatggta ccgaagccta tagattctgt tctgacagaa
     1321 aaataagcga cgaggcgttc aatactttga aggcagaaca ggacttagaa ctagaaaaaa
     1381 ttgctaatac ctaatttgct aatgtgcaat ttgctaatca acaaattagc caactaataa
     1441 atactaactt ataaataaat actaaataac aataaatact aacagataat aagcatcatc
     1501 atcaacatca ggaggagcta tggacgaaaa aaagcttttt gaaaatttcc aattaacttt
     1561 tggacggatg atatcgccat ttgaaatcga agatattcaa aagtggattc acgaagataa
     1621 catgccaatt gaagttgtca accttgcctt aagagaagcg gtagaaaaca acaaaatcag
     1681 ttggaagtat atcaataaaa tcttagttga ttggtataaa tctggagata cgacagtaga
     1741 aaaggtcaga gacaggttgc aacggtttga cgatagtaaa aaacaacgaa gtgtaactac
     1801 ctcaaacgtc ccaagctggt cgaatccaga ctacaaagaa ccagatttaa aagaatttgc
     1861 tctaggaagc atggacggta tagaagatgg atcaggagat ttttaatttt tttaacaaac
     1921 aaatcaaaaa aagattttgg taaaacggcg agtaaagaga cttttgctaa gtttgctagt
     1981 tactgcgccg aaggaatcga aaaaaatgga gttaagccaa tttttaattg gataaaccta
     2041 tacgcttttg gaactggtat gacaacagca gaagcagacc ggttgagaat agagcgatat
     2101 aaacaggaga atgcgttatg acaaaacaac atagagaaac gcttatctgg taccgagcaa
     2161 gtcatcaaga gcgtgagaga ttgcttgatt ttggactagt tgataaagca cggtacgtga
     2221 cactattgcg gcaattgcgt aagaaatatg cgatttagga ggaaatatga caccagaaca
     2281 agcagaaaaa gcaaaaatca gagctaaaca agaacttgaa acgtttagca tataccttga
     2341 tcaggcaatt gatgatctcg gtggagttct aacttcacga gaagtctttt tagcagcggg
     2401 aataacatat cttggcgcag gtcagacaga tatacatgct gcagtcgagg gattatgtga
     2461 gcaaatccaa tgattttaaa tttagtgaag actgggagag caactaaaac acaaaaagag
     2521 gaaatgaaat gaacatcaaa gaaaaaattg tagtgctaag aaacactgaa gacggaagtt
     2581 ttttaaagag tttcaaaaac aaaaaagatg tacttgctta taatgtggaa cttacagata
     2641 gcattcaact ggcatcattt ttaccagaag aagcttacaa catacaaaaa gaaaaaattg
     2701 ataatttggc agaaacgctt gggtgtgatg ttgtagttat cgaagcatca tatgacctaa
     2761 aatttattga tggcgaatca gtgccagagt taacaaaaga gcaaaaagtt aaaagtatgg
     2821 taaacggaat gtttgagcag gtttttggag gtgaatagag atggcaaatc aattatcaac
     2881 atacacacac aaagatacta ctgatggacc attgatgatt ttacgttctg acaaagatac
     2941 gtttgatcag tcagctcaat ttgtggatta cagcggtaag actaatgctg taaatattgt
     3001 aatgcgccag ccaagcgcac ctaatttttc ctcggcactt aatataacca gtgccaacga
     3061 aggcggtagt gcgatgcaaa ttagaggcgt cgaaaaagcg ctaggaacgc tcaaaatcac
     3121 acacgaaaac ccaaacgttg aggcaaaata cgatgaaaac gctgcagcgt tatctattga
     3181 tatcgttaaa aaacagaaag gcggaaaagg tactgctgct caaggaatct acattaactc
     3241 aacatcaggc acagctggta aaatgctcag aatcagaaat aaaaatgaag acaaatttta
     3301 tgtaggtcca gatggcggct ttcactcagg tgcaaattca actgtagctg gtaatctaac
     3361 agttaactat ccaacatctg aaaaacatgc tgcgactaaa aaatacgtag atgaaaaaat
     3421 tgctgagtta aaaaaactca tactaaaaaa atagattaag gaggataaat gagcagagac
     3481 ccaacatata caataaacga gcacgactta tcttttgcag atggtcgttt ttatgtgacc
     3541 tttaaggcag ataagtcaag tgagactgtg agacttaaca gtagttgcct tggcaatacc
     3601 ataatcaaaa agctacaggt cgaggatgac aatacaatgc acgactttgt aaagcctaaa
     3661 gttaccactc aacaagcttt tggactagct cagcaggtca aagagcttga tttacagcta
     3721 aaagacccta agtcagattt gtggggcaaa atcaagttca ataataaggc aatgctagtc
     3781 gagtacgcca acaaagagat gtcaagtgcc attgcgcaat cagctgagca gatattgtta
     3841 caagtcaagt ctattgatga tgaacgatat tccaaatttg agcaaactct gaatggtatc
     3901 aaacaaactg tcaaaagtga gtcagttgaa tccgcacgta ctcagctagc atcaatgttt
     3961 gatagtcgta ttagtggact tgatggcaaa tacagtcgtt taagccaaac aattgatagt
     4021 cttagcagtc gtcttgatga tggtgttggt aactactcaa cgctatctca aaaggtaagt
     4081 ggcattgatt tacgagttag taatgcagct aatgatgttt ctcgattgtc tcagacagca
     4141 caaggattgc agtcacaaat cacaaatgca aaccaaaatt acagcagttt gtctcagact
     4201 gtagggggtc tacaaacaac tgtacatgat aatcaatcaa atgctacaag tcggattaat
     4261 caattaagtg atttaatcag tactaaagtg actaagggcg acgtcgaaac aactattgct
     4321 caaagttacg acaagatagc cttcgcaatc agggataaac tcccagcaag caagatgact
     4381 ggcagtgaga ttatctcggc aatcaatctt gataggtctg gggttaaaat cactggcaaa
     4441 aacatcacat tagacggtaa cagctacatc agcaacgctg ttatcaaaga tgctcacatt
     4501 gctaacatgg atgctggtaa gattaacact ggttatctta atgctagtag aattgcggca
     4561 gaagctatca ctggcgacaa aatcaagatg gactatgctt tttttaataa gctcactgct
     4621 aatgagggat attttaggac cttatttgct aaaaatatct ttaccacatc tgtacaggct
     4681 gtcactacgt ctgcgagtaa gattacaggt ggtgtgcttt ctgccacgaa tggtgcgagt
     4741 aggtgggatt tgaatagtgc aaatattgat tttaatcgag atgccacaat taattttaac
     4801 agcaaaaaca atgcgcttgt tcgaaaatca ggtacccaca ctgcttttgt acattttagt
     4861 aatgcgacac caaaaggcta tagaggctca gcgttgtatg cgtcaatcgg gataacctca
     4921 tcaggagatg gcatcgacag cgcttcgtct ggacgtttct gtggagttag gtttttccgg
     4981 tacgctgaag ggttacagca tacagcaaag gtcgatcaag ccgaaattta tggtgatgat
     5041 attgtcttta gcgacgattt taacatcgat cgtggcttta agatgcggcc tagcctaatg
     5101 ccaaaaatgg tcgacttaaa caagatgtac caggcaattt tggctctcgg ccgctgctgg
     5161 ctgcatgcta ataacacggc ttggtcgtgg aattttgata cacgcagcgc aatcatcgca
     5221 gaatataacg cacacattaa taacttatag gagaaacaat ggatttaacg cttaaaaaca
     5281 aagatttaaa cacactatat agtgtactag acaaaatcaa aatcacgaac atgcgagcaa
     5341 accgcggacg tgctaagcta ctcgcaaaag tagtagataa attcaaagag tacgccaagg
     5401 atgagggtga ccttattgat ctgtatgctc aaaaagacaa agatggcaag tttgtcattg
     5461 atgagcacaa aaacatcaag ctagcagacc ccgctaaact cgacgagttc aacggcctac
     5521 tcaacgagct agctgatgaa gaaattgtga ttaaaggggg tgagtactcc aagcgattta
     5581 tcgatttttt ggaatattta gccgaatctg aagatgagtt tacagctcaa gaaatcatta
     5641 ttgttgacag catcttagag caatacgaag gaagcaaagg agaataacta tgaagacatt
     5701 aacactatca ggcaaacctt atccaattca tgaaggcggt aaagttgtaa aaacagaggt
     5761 tcgcttaatc ggtgacaatg ggctatttat ccccattgaa ttaatcggtg atcagacagc
     5821 taagggagca gatgacctta ttaaagaggg gctagatgct tttgtacgcg agtatgtgac
     5881 taaatacgcc gtagcagaat cagtgcaaaa agtggaagag ttgagcctcg cacaaaaaga
     5941 gattgagcaa aatgcggagc aagcaaaggt aacagcagaa gccgctgaaa aacaagctaa
     6001 atctctggag cttgtcattg caaaatctca aaaaatggct aatctacaag caatccatct
     6061 actaacaagc ggaagcaaag tggaacccga tatttacaaa ggtcttttag agctaatcga
     6121 gccagccaaa caaggtgagt atcaagccta tgatgtcttt acggtagtag acgagtcgca
     6181 cgaagagcag gcaggagaag gtaatcttgt ctttgtacac gttaacgagc catttactta
     6241 tgacaaacag acgcttaaag agctagagga agaggataaa gtcacagtca ttaagtatgc
     6301 ggacttagtt aagcaggatt agcgaggtaa gttatggcaa cagagttgat atttggcctc
     6361 ggcggcttta ttttagctat cgtcacgact tacaatattt ttaatgcaaa atccatcaag
     6421 catgcgacag atattacttt gttgcagtct gaggtagagc atttaaaaat tgtcactcgg
     6481 caaaatgcta ggcgtcttga ggagcatgat gagcaaaaca aaacgctcat cacaatgaca
     6541 gagcaaatta aaaacctcaa tcgtgaggta agagaactta aagatattat gagaggcgaa
     6601 gcatgattaa tttaaaatta cgactacaaa acaaagtaac cttgatggct attttaggag
     6661 ctatcttttt actggcacag caattaggta ttaaactacc atcaaacatc gcggatattg
     6721 caaacacagc tgtaacgctt ttggtattgc tcggtgttgt cacagatcca accacgaaag
     6781 gcctgtcaga cagtgagcaa gctttgactt accacgaacc aaaacaatag gaggggacat
     6841 gcgggcaatc acacgattag cattagttat agcaatagca atactgtatg tgccattatc
     6901 tgttgttgct ctgatctttt atccattttt agataaggag gatgatagat ggtagtagat
     6961 accgaaaaag ctatcgcttg gatgggttta aaaaagggtc gtgtcagcta ttccatggac
     7021 tgccgtaatg gtcctgatag ctatgactgc tcaagcgcta tttgtagtgc tttaatctat
     7081 gcaggagcta gtaatcctgg ttggctactc aatacagagt atatgcacga ctggctagtc
     7141 caaaacggct ttgagttaat cgctgataat gaggattggg atagccaacg ggctgacatt
     7201 gctatctggg gattccgtgg gcaatcagca ggcgcgggtg gtcacgttgt gatgtttatt
     7261 gatgctgaca atattatcca ctgcaattac gcaaataata acatcacgat tgataactac
     7321 aatcaaacag cagcagcgag cggttggatg tactcttatg tttatcgcta tacaggagca
     7381 aaagcccaac ctgctacaaa taaaagcatt gatgagctag ttcaagaggt cctatctggc
     7441 aaacatggta gtggcgagca gcgcaaaatc tcacttggta ctaactatga tgccgttcaa
     7501 gcaaaagtaa acgagatgct caaacaacca caagtagcgg agcaaagccc tgctgtcaaa
     7561 caagacggag acttattgtt taatggtgct gtgcttaaaa agacagtctt ggataaaatc
     7621 ctagctaaat gtaaagagca tgacatcttg ccaagttatg ccattactgt gttgcatttt
     7681 gaggggcttt gggggcaatc tgctgtaggc cgtactgaca acaattgggg aggtatgacg
     7741 tggactggcc aaggcaaccg tccaagcggc ataacggtaa cgcaaggtac ggaaaggcct
     7801 gctgttgagg gtggtcatta catgcattat gctagcgttg acgacttttt gacggactgg
     7861 ttttatctct tgagagctga tggctcttat agggtaagcg gtgctaagac tttcagcgag
     7921 gctgtgaaag gcatgtttaa ggttggtggt gcaacctatg attatgctgc aagcggattt
     7981 gatagctaca ttgttggtat ggctggcagg ctaaaagcaa ttgagcagga aaacgggtca
     8041 cttgccaagt acgaccaaca ggccgacatt gatgtcggtc agtctgataa aattgacgtt
     8101 gttattgaca gcttggaaat taccatcaac ggtgtgactt acaccgcaac taaaaaacca
     8161 atttaggagg taaagctccg agataagata aaaccgctca gataatttct gggcggtttt
     8221 ttattatatt ttatcttgtt ttttgaaata aaaacaaata taatatataa aaacgcaaaa
     8281 aatatcttaa ggagtaggat tatggcatta cagggaataa aaaagttacc agtcttgcta
     8341 gaggataagc aagccttaag cctttataag aaactggcaa tagtaaataa ggttctgggg
     8401 aagttagatg ctgttttaga atcatccatt attaactctt cgattctcag tttattatct
     8461 tacaatgagt cagtacaatc gacaagaata gaagggactc aagtaacttt ccatgaaata
     8521 atggagactg ctaaagtagg tgccaaaaat tggcagcaac gagaagtttt taattataaa
     8581 aaggcgattg attttggttt tcataaaata aaaaaaggcg atgtcataac tacacggttg
     8641 ataaaagatc tccatcgcct tttgatgtct gatgaagcta gaggaactac atctaacggc
     8701 ggagagttta ggaagattca aaattttatt ggtccagata aggatatcga aaatgcttcg
     8761 tatatcccca ttcccgcaaa cgaaattggt gcatttatga caaatctcga attttttata
     8821 aatggggaat atcattcgag tttagagtgc gggaggactc aagaatctat taactttaat
     8881 agtgatattt tattgagaat tgcggttgct catgcgcaat tcgaatcaat tcatcccttc
     8941 ttagatggta acggccgtct tgggagaatt cttattgcat tgatgtctgt tcaggagggg
     9001 cttctaaaac acccaatatt tttcgttagt gaagaattag aaaaggaaag aattcgttat
     9061 tataacgcgc ttaatgctac aaggggagag aatccagatt ggaacttatg gcttaatctc
     9121 tttctcaatg ctagtgagca aatggccaag aatatcttaa agaaaattcg aaatgcggat
     9181 gaacatgcca aaaaaggact ttctgtttgt gtgactcaaa ctcaaaaaac agtttggctt
     9241 gctactttta gttttcctgt atcaactgca aaacagttag ccgaggctac ggagttccat
     9301 ccagcaacag tgaaaaaagc tttggacttc ttggtaggag agggattgtt ggataaagat
     9361 aattcagtaa aacgaaatgt tccgtattac aactatgatt taattcgtgc gatacaaaat
     9421 tattaatata cagacacttt tacctctata ctgatccccc tgcacattca tag
//
LOCUS       DAUELZ010000065         9347 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.066, whole genome shotgun sequence.
ACCESSION   DAUELZ010000065 DAUELZ010000000
VERSION     DAUELZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.066"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(99..310)
                     /locus_tag="VHI09_001205"
                     /pseudo
     CDS             complement(99..310)
                     /locus_tag="VHI09_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368983.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
     gene            complement(420..836)
                     /locus_tag="VHI09_001206"
     CDS             complement(420..836)
                     /locus_tag="VHI09_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121084.1"
                     /translation="MRFKDFNLEVVNVERYSSDYSMTVNKNFVTFSKGIVQALEYPAH
                     VLVAFNKDTKVMGIQVCRAKTRGAFSFSKPVGEQKGIVQVGHKTLKETLLTIMSEWKS
                     DKRYRVEGIHIPEDKAFVFELKDFDELSDFRKNDNR"
     gene            complement(851..1963)
                     /locus_tag="VHI09_001207"
     CDS             complement(851..1963)
                     /locus_tag="VHI09_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ6121085.1"
                     /translation="MVRRNSKITRQQKKIRDAFVSERTVEIIPAKREFTDVKTKKLRV
                     AAYCRVSTFDESQSGSFELQKQTYTERINSNPDWIMAGIYADQGASGTSIKRREQFQQ
                     MLHDCRCGKIDLIIVKSVSRFARNQLDFISIYRELKALSPPVGIYIEDINLNTLDTNS
                     EFILGIMAIVAQGESEQKSASITWSVIERFKRGIPMIPTHNLLGYTKDQYGRVVIDET
                     EAKIVRLIYDSYIEGMTASEIASTLMTNHIPTVTGLERWTSLAVYNILRNEKYKGEII
                     MQKTYTVDCFSHKTRKNNGEKPKYRLKNGIPSIIPESRWDLVQELLKQPRRKSKSTSE
                     IFVPKLYIKKLKSGKLRDFVVLDPSWKSEDIHEVFK"
     gene            complement(2092..2748)
                     /locus_tag="VHI09_001208"
     CDS             complement(2092..2748)
                     /locus_tag="VHI09_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HEQ6121086.1"
                     /translation="MMKRKIRMTTIFLTRTSCSNCGKQSTFERFDRVYAAKTPEIISA
                     ILDWDFFKFTCHNCNHKVLIDYPTVVVDEEQKTIIQYCADGNVDVLSMQICSLISEGV
                     NLSEYRIRVVSDIESFVEKVQIVSVGYDDRAIELMKYMNSPLEDGDIQFNYEHMVFTK
                     VGHENYQFMFINNQIAVASLDFSQEQYEYYLADVEDLETNTYYIDSRWAESFFRTSLA
                     "
     gene            complement(2853..4538)
                     /locus_tag="VHI09_001209"
     CDS             complement(2853..4538)
                     /locus_tag="VHI09_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEQ6121087.1"
                     /translation="MTFSWTKLVPLSKIQFAFLILVLFYQIHSPSWLTFLLSLSLICL
                     LVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLEIQPVQITSVALVPDSIRINGDQ
                     LAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
                     FLAYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLL
                     FGYLDTSFGEMTDYYSQLGIIHLFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELP
                     FACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLT
                     FSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTALLSYLF
                     DLFILPLLCLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLIL
                     VISFAIFYDYRQVRQRVITCGLVIALTLLSVKYPLTNEVTFIDIGQGDSILVREWTGK
                     NLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHAEV"
     gene            complement(4519..5181)
                     /locus_tag="VHI09_001210"
     CDS             complement(4519..5181)
                     /locus_tag="VHI09_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEQ6121088.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDNSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            complement(5381..6121)
                     /locus_tag="VHI09_001211"
     CDS             complement(5381..6121)
                     /locus_tag="VHI09_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEQ6121089.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKLAKVKIMPAAYQGPMSVKRLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTMLFSYIASFVWNPDKHRH"
     gene            6161..7015
                     /locus_tag="VHI09_001212"
     CDS             6161..7015
                     /locus_tag="VHI09_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEQ6121090.1"
                     /translation="MKADFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIVEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGAAS
                     KGKPNHDH"
     gene            7005..7283
                     /locus_tag="VHI09_001213"
     CDS             7005..7283
                     /locus_tag="VHI09_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEQ6121091.1"
                     /translation="MTTKKAYMYVLECVDKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(7307..9308)
                     /locus_tag="VHI09_001214"
                     /pseudo
     CDS             complement(7307..9308)
                     /locus_tag="VHI09_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
BASE COUNT         3054 a         1920 c         1585 g         2788 t
ORIGIN      
        1 gccaaagggg gttagccctt aaattgcggt taatattcta tatctcttcg tcaatctcaa
       61 tccctgactt gaattcaacg gtgagcttat cttcatataa tttttttact agcatttaga
      121 attcggttga ttgtacgatc gctatagtcc tcattagctg ctgagggatt catacatata
      181 gccacgagtg gattcgttcc taagcatccc agtaggaatc tacggtaggc attatatttt
      241 tcaggaatac atagattagg tcttctccca aaggataggc tagtctgact tttgtaactt
      301 tatcgttcat agagttacct cctacagtat attttaacat gaaccccctt gcaaggatat
      361 gaaaatggca ggaatacaat agttgtactc ctaccaataa tttttatctt cgttccttac
      421 tatctattgt cgtttttacg aaaatcagat agttcatcaa agtccttcaa ttcaaaaaca
      481 aaagctttat cttcaggaat gtgaatcccc tctactctat aacgcttatc agatttccat
      541 tcactcatga ttgttaagag tgtttctttt aaagttttat gacctacctg aacaatacct
      601 ttttgctccc caacaggttt tgaaaaagaa aatgctcccc tagtttttgc acgacaaact
      661 tgtataccca ttacctttgt atccttatta aaggcaacca gtacatgtgc tgggtattct
      721 aaagcttgga caattccttt actaaatgta acaaagttct tgttcaccgt catgctataa
      781 tctgatgagt aacgttcaac attaacaact tcaagattaa aatctttaaa acgcataatt
      841 tacctctttc ttatttaaaa acttcgtgaa tgtcttccga tttccaacta ggatcaagta
      901 caacaaaatc cctcaactta ccagatttga gtttcttgat gtagagttta ggtacaaata
      961 tctcagaagt agatttcgat tttcttctag gttgcttgag taattcttgt actaaatccc
     1021 atcttgattc tggtatgata gagggaattc cattttttaa tcgatatttt ggcttttcac
     1081 cattattttt ccgtgtttta tgactaaaac aatcaacagt atatgtcttc tgcatgatga
     1141 tttcgccttt atatttctcg tttctgagaa tattataaac agctaaactt gtccaacgct
     1201 ccaaaccagt aactgttggt atatgattgg tcattaaagt agaggcaatc tcacttgcag
     1261 tcataccttc gatgtacgaa tcatatatta ggcgaacaat ttttgcttct gtctcatcta
     1321 tcactactcg accatactgg tctttcgtat acccaagaag gttatgggta ggaatcattg
     1381 gtatccctct tttaaagcgt tcaatgacag accatgtaat tgacgcactt ttttgttcac
     1441 tctcaccttg agctactatg gccataatac caagaatgaa ttcactattc gtatcaagtg
     1501 tattcaaatt tatatcttcg atatatattc caactggagg ggatagtgcc ttcaactctc
     1561 ggtaaatact aatgaaatct agctgattac gagcaaacct gctaacactc ttaacgatta
     1621 tcaagtcaat cttcccacat ctacaatcat gaagcatctg ttggaattgt tcacgtcgtt
     1681 tgattgatgt tcctgatgca ccttggtcag catagattcc agccattatc caatcaggat
     1741 tactattgat tctttcagta taggtttgtt tttgaagttc aaaactacct gactgagatt
     1801 cgtcaaaagt acttactcga caatatgcag caacacgtaa cttcttagtt ttaacatcag
     1861 tgaactctcg ctttgcagga ataatttcaa cagttctctc agatacaaat gcatctcgaa
     1921 ttttcttctg ttgacgggta attttagaat tccgtcttac cattggtttt acctcctttc
     1981 tagaaatcta cataataatt atatatatta actacgtttt tctaaattag aagataaaaa
     2041 atgacctaac caaaaatcag gccatttata cttataaata gattttgaat tttatgccaa
     2101 actggtacga aagaatgatt ccgcccatct agagtctatg taatatgtgt tagtttctag
     2161 gtcttcaaca tcagcaaggt agtactcata ttgttcttga gaaaagtcga gagatgctac
     2221 tgcaatctgg ttattaataa acataaattg atagttttca tgtccaacct tggtgaaaac
     2281 catatgttca tagttaaact gaatatcacc atcctcaaga ggactattca tatacttcat
     2341 aagctcaatt gctctatcat cataccctac tgaaacaatc tgtaccttct caacgaaaga
     2401 ctctatatcc gaaactactc gaattctata ctcactgaga ttgacaccct ctgaaatcaa
     2461 agagcatatt tgcattgaaa gtacatcaac attaccatct gcacaatact gaataattgt
     2521 tttttgttct tcatctacta cgactgttgg gtaatcaatt agcaccttgt ggttacagtt
     2581 atggcaagta aacttaaaga aatcccagtc taaaatagca gaaataatct caggagtctt
     2641 cgccgcataa accctatcaa atctttcaaa tgtactttgt tttccacaat tgctgcagct
     2701 tgttcttgtt aaaaaaattg ttgtcattct aattttcctc ttcatcatta ttaagtaagc
     2761 aacttgctct cacttattat attcgcaatg caaaataaat tttagaattt ttttcaaaaa
     2821 aaagttaaat tgtattgtat caattttaga actcacactt ctgcgtgagt tactaatagt
     2881 tggtcaattg tgtgaatacc tctacttttt aagtaaggaa ttaaggtttt ctgagcatta
     2941 gctacgtgat gtcctcgtcg ccagtgctct tttgaagaaa agaaagggcg cccacctaca
     3001 tctattaaga gatttttccc tgtccactcc ctcaccaaaa tactatctcc ctggccaatg
     3061 tcgataaagg tcacttcatt ggtcaaagga tatttgacag acaaaagcgt caacgctata
     3121 accaaaccgc aagtgataac tcgttgtcgc acctgccgat aatcataaaa aatagcgaag
     3181 ctaatcacca agataagcaa gtgccaactg gtcgggcttc caaaaacaag ggaactattg
     3241 aaagtatttc cgagaaattg aatgactttt tctaataaaa taaaaagatg gttgcatatt
     3301 gagacagtca ccaaagggga tagacaaaaa accagacaaa gaagaggtaa aataaaaagg
     3361 tcaaagagat aagacaagag agctgttaag accattgaca ttgggttaaa actggaaaaa
     3421 taaaagagca aaaatggtaa tattcctagg gaaatggtca agacttgtac caactgtctt
     3481 ttagctcctg ataattcttc aacagtaacc actgttaaca aaaaagcata gctaaacgtt
     3541 aatacgccac caacagtcat taagaaatgg gcatcccata aaaagacaag taaaaaagta
     3601 caagcgagat tgtctaatcc ttttattcct aaatgacgca actgcgactg cacgaggctt
     3661 cgaataacag aaatactata acctgtcaac gcggcataaa agcaagcaaa aggaagctct
     3721 atccatttta tccattccaa aggaactgcg agtaatagca acacccttct aaaacaagta
     3781 agaaagaagc caacctgcat ccctgataaa gcaaagagat gaatgatacc caactgacta
     3841 taataatctg tcatctcacc aaacgacgta tctaaataac caaaaaggag gcccgtcagg
     3901 tagtgagaca taggtcttgg aaaatgctgc tggcaatgaa caatggctcg tcgtcttaaa
     3961 gaggacaaat agtcacatat tgactcgggt gaaatcactt caagctgttc tatttgctct
     4021 accttcccta tcctgtaaat tccttgataa gccaaaaaag tctgataatt aaagccttta
     4081 aaatttcgaa cttcctcagc tttttccaat gtcacttttg cgtgcatcac taaccaacgg
     4141 tgctctttct tgaacaattg ggcttctgct tggcttttca aacggtaaaa taactgataa
     4201 gaatgtttcc catgacgtcc aagaacagct aattgatcac cattaatccg aatactatca
     4261 ggaactaatg ctacagaggt tatttgtact ggctgaattt ccagtttttg aaccaactgc
     4321 tgtttctgat acagcaaaaa caaagcacag aaactaagga tggcaaagac accgagaaat
     4381 tcttttttag acaaacgctt gacaagtaag caaatcaaac taaggctaag gagaaacgtt
     4441 aaccagctag ggctatggat ttgataaaag agaaccaaga ttaaaaaagc aaattgaatc
     4501 tttgacaggg gcactagctt agtccaagaa aatgtcatct tttaatttct cgagcgtttt
     4561 ttcgccaatc cccgaaacct gacggagatc ttctaacgct ttaaaaccac ctaacttatc
     4621 tctcatatca ataatatcct gagctctttt agcaccaata cctgggatat gttgtagttc
     4681 ttctaagctg gccttattaa tatgaacctt tgactgatcc ttgctggcag tttctttttt
     4741 accagaaact aatgatcttg gtagcacaga aatctcttct ccttgtttag gtacataaac
     4801 aacttgctca tcagttagtt tttctgcaaa attaatagca tgtttatcag cttctgaagt
     4861 taggcctcca gctagttcaa tgacatctcg cacacgactg ctagcagtta gcttataaac
     4921 tccctctttt tgtacggcgc cttttaaatc cactaaaata ctgttatctt cttgaatttc
     4981 ctttttttct tcactaacct gctgtttcac aggcgatatt tctctcaaag caggaacggc
     5041 tgccttgctc tgcctatttc cccagaacaa aaaactgaag cttagtatta gaaaaagtcc
     5101 taataaaaga ctaattaggt atggcagaaa ataccgctct ctcaaaaacg attttccttt
     5161 aattaataat tcgtctaaca tttttttacc tcatcttttt attcgtaaat gatatccaaa
     5221 agtatagtag tattgtcatt ttttgtgctt aaaagagtta agggtatcat cgaatatctt
     5281 ttttagaaat gaaaaaagtg attaagcatt agcggcttct ttcaatagaa gactaatctc
     5341 aatcacttct tatttcaggc acatgttagc cctcatgttc ttaatggcga tgtttatctg
     5401 gattccaaac aaaactagca atataggaaa ataacatggt taataataat accaccacca
     5461 aaaccaatcc taatggtaaa cggtagagat aagttaaagg gttccttgct ttgcctggtt
     5521 ggaaaggtgc caactcgtca tccatccgat caaactctgc ctgaatacgg tttgccacct
     5581 cagcaatccc ttcatcattc atgcgtttaa tatctgagac gtcaatggga ttcccaaaag
     5641 tcatgtcaac ccgttctccc gctagtaatc tcttgaccga cataggcccc tgataggctg
     5701 ctggcataat cttgacctta gccaatttag caatcacagc gacccctcct ttgacctctt
     5761 gcgaatgcct acttccactt ggaaacataa gaagagaacg attgctcttt ttgagcatat
     5821 taacagggta acgaatagca tctggactcg gtttgtcacg atcaatagga aaagcaccac
     5881 acattttaat ccaccaagca aagagacgat ttgcaaacaa ctctttttta gccataaaaa
     5941 taaactgctt aggacgtgct gcaaatgcca tgtaaactgg atcccaaaag gttcgatggg
     6001 gtgctaccag aatataattc tcagaagcat ctaacatctt ttcttcatga tggtagtggg
     6061 cattgccatt gacaacccat aataaaaaga ctactaaacc gcgtaagtaa gcgtaaaaca
     6121 caggcttctc ctttaataga ataatcttac ttattgtatc atgaaagctg acttttcgaa
     6181 agtcaaaatc actcttcaaa cattcacctc aagcctattt tatgttaaaa taacaggtat
     6241 gataaaagca atcttaaaag aaggcgaacg tattgatcaa ctcttttcaa gtgatgttgg
     6301 cattattcaa aacaaggatg tctttagtta ctctattgat agcgtcctct tatcccgctt
     6361 ccccaagatg ccttccaaag ggttaattgt ggatttgtgc tcaggaaatg gggcggttgg
     6421 cttatttgct agcactagaa ccaaggcagc catcgttgag gtcgaattac aagaacggct
     6481 tgctgatatg ggacaacgct ctatccagct caatcaactg gaagaccaag taaccatgat
     6541 ttgtgatgat ttaaaaaacc tcctaaacca tgtgccacgt tcaggagttg atttgatgct
     6601 ctgcaatccc ccttacttta aaagtcacga aagttctaag aagaacgtct ctgaacatta
     6661 tttactggct cgtcatgaag tgaccactaa tttagaagaa atttgtcagg tagcccgtca
     6721 tgcccttaaa tcaaatggtc gtctggcaat ggttcatcga ccagatcgat tcttggaaat
     6781 tatagatagc cttagagcta atggtcttgc ccctaaaagg gtacaatttg tctatcctaa
     6841 attaggtaaa tcagctaata tgttacttat tgaagcaatc aaagacggtt ctattgaagg
     6901 gatgactatt ttacctcctt tagttgtcca caaggaaaac ggagaataca ctgatcatat
     6961 ttttgagatt tattttggtg ctgcttccaa aggaaaacct aatcatgacc actaaaaaag
     7021 cttatatgta tgtgttagaa tgtgttgata aaaccctcta cactggttat acaactgatt
     7081 tgaaaaagcg tttagctaca cataacgcag gaaagggggc taaatatact cgctaccggt
     7141 tacctgtttc tttactttat tacgaagtgt ttgatagcaa agaggctgcg atgagcgctg
     7201 aagcactctt taaaaaacga aaaacaagaa gccagaaatt ggcctatatt gctactcatc
     7261 aaaaagaaaa gaaaaatcat tgagacttat catctcaatg atttttttat gcttcactgt
     7321 cttccacagg caccaattct ttgattggta acttcaagac atctgataga atcaagggaa
     7381 ccacttcaag ggttacttct gaatattgaa gcccaaacca acgcattgga ctagcggtaa
     7441 catgtttaat gcttgcctta gtctgcatga taaagcgttc aaagttactc aattgacgtg
     7501 cattggagac ccgttcttct attaaaacaa agcgaaaatc accaacatca cgaccaggtg
     7561 tgatggtata ttcctgttct tgtttgggta aacggccact ttccatcaaa tcctgaacaa
     7621 tcgtgcgcaa atagcgtggc acagtctgtg gcattctaaa accaagataa aggttgactc
     7681 gaaccatata atccgttccc atcatatcta ccttgtactt agctgtataa ggttcatcag
     7741 ttacctggac atttacaaac caataaactt gagcgcgttt tggccgcttg tctaaaatgg
     7801 agtataaaat agaccggtca atcatatgat cctgcatacg attggatagg tagacaacat
     7861 ttgtttggta gaggtcaaaa caaacgtcgt cacgaagttg tttaatctgc tccttgtaat
     7921 cattcaaatt aagagatttc acatacttga aaacaatccg tgtgcccgca tgccagataa
     7981 acatgacaaa gacaattgca agtgccaaga taacaaccgc atagccaccg tgcataaatt
     8041 tgatagctga agccaagaag aaaatgaatt ctacaagggc aaagaaagcc atcgctaaat
     8101 gagccaagat gggtctcgtt cctttcttga ttaagtaata ctttaagagg attgttgtca
     8161 tcaacatggt gatggtaata gcaagaccat aagctgcctc catgtgggct gatgttctaa
     8221 aagccaaaac agtacaagag gtcactgcaa ataaaatcca gttaatgaca ggaatataca
     8281 attgccccaa atttgcccct gggtaagtca cacgaaacaa agggaaaatt ttcaaacgca
     8341 tggcctctga aaccaaggta aaggagcctg aaataagagc ctgtgaggca ataatagctg
     8401 ccaaggttgc caaactaaca aggtaaactc ttaattgtga tggcacacta gcaaagaaag
     8461 ggtttagttc aatacctgag tgtttattcg ctaaaatcca cgcagcctgg ccacagtaag
     8521 accacacaat acacatctta acaaagggcc agctcacata aatatttcct cgaccaacat
     8581 gtcctaaatc cgaataaagg gcttcagctc ctgttgtcgc aagaaaaatg gaccccaaga
     8641 taaaaatccc acgatggttt tcaggactaa acagcaaatg cagagcatag taaggattga
     8701 tagccttgaa aatttccaga tgccccaacg tattaaaaaa accactaaca cctagaaaac
     8761 taaaccagat aaacataact ggtccaaaaa ttttaccaat aaaacctgtt ccaaaccgct
     8821 gaataccaaa caagactatt aaaatcacta gagttgtgat aatgatattg gtttgatttt
     8881 ggtagatgtg actcagacca ggaacggctt tcaagccctc aatagctgaa gtaactgtta
     8941 ccgcaggtgt aagaagcccc atcagacaaa agtgttgcac ctccaatcat cgccggaata
     9001 atgagccacg gtgacatttt acgtaccaag gtgaataaag aaaaaatccc accttcatgg
     9061 tggttatccg cttttagagc aatcaaaacg tacttgattg ttgtgattaa ggtcagtgtc
     9121 caaataatta aagagattga tcctaaaata aaggattcag aaacctgatt aacccctcct
     9181 tgattttcaa ctagagactg catcgtataa agggggcttg ttccaatatc tccataaaca
     9241 atacctaaag caataataaa acctgctttt gaagctttgt caaaggcagt aagatggcta
     9301 tcagatatct tatactcctc ctaatttttt aaaaattatc acaaaaa
//
LOCUS       DAUELZ010000066         8975 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.067, whole genome shotgun sequence.
ACCESSION   DAUELZ010000066 DAUELZ010000000
VERSION     DAUELZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.067"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..257
                     /locus_tag="VHI09_001215"
     CDS             <1..257
                     /locus_tag="VHI09_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121092.1"
                     /translation="NYLVVFQLLSLYYHYDYHYMTSLYPENSRSKKKNMLSFLRGLSF
                     LMLIVNMLCCSSAPKALILIVGMVFIACIYLPYKLKKIID"
     gene            321..1112
                     /locus_tag="VHI09_001216"
     CDS             321..1112
                     /locus_tag="VHI09_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEQ6121093.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            1112..1747
                     /gene="trmB"
                     /locus_tag="VHI09_001217"
     CDS             1112..1747
                     /gene="trmB"
                     /locus_tag="VHI09_001217"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEQ6121094.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            1812..1898
                     /locus_tag="VHI09_001218"
     tRNA            1812..1898
                     /locus_tag="VHI09_001218"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1846..1848,aa:Ser,seq:gga)
     gene            2006..2542
                     /gene="rimP"
                     /locus_tag="VHI09_001219"
     CDS             2006..2542
                     /gene="rimP"
                     /locus_tag="VHI09_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEQ6121095.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIAITSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            2717..3874
                     /gene="nusA"
                     /locus_tag="VHI09_001220"
     CDS             2717..3874
                     /gene="nusA"
                     /locus_tag="VHI09_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEQ6121096.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATVVEEPVVDDVMTAEEV"
     gene            3890..4186
                     /locus_tag="VHI09_001221"
     CDS             3890..4186
                     /locus_tag="VHI09_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEQ6121097.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            4179..4481
                     /locus_tag="VHI09_001222"
     CDS             4179..4481
                     /locus_tag="VHI09_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEQ6121098.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAIADAGFSKKMRTLME"
     gene            4501..7362
                     /gene="infB"
                     /locus_tag="VHI09_001223"
     CDS             4501..7362
                     /gene="infB"
                     /locus_tag="VHI09_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEQ6121099.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIDR
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKVLGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            7568..7918
                     /gene="rbfA"
                     /locus_tag="VHI09_001224"
     CDS             7568..7918
                     /gene="rbfA"
                     /locus_tag="VHI09_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEQ6121100.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRELDKKD"
     gene            complement(7971..8972)
                     /locus_tag="VHI09_001225"
     CDS             complement(7971..8972)
                     /locus_tag="VHI09_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ6121101.1"
                     /translation="MKKTLKSPLILLGLTLAIGASTQVDASSRSWKSWFIEKYFWLKG
                     DKGYYSEQDETKFDQYVNSSGEASNKGFDLKEEQVNGTLVTKTVDNMQVHTWNENPSN
                     KANQKVIFYLHGGSYLNNPTTYHIDMLKTLSTSLDAKIILPIYPKAPTYSYSDAMPKL
                     VDLYRDTLKETTAQNITIMGDSAGGGLALGLALALKNESLKQPKDIVLLSPWLDVTMS
                     NPDIESYESHDPILSAWGLRKVGEIWARRDPNNTKHPYVSPINGDVSGLAPITMFVGT
                     HEIFYPDVKKFDDILTAKGHQHELIVADKMNHVYPIYPIEEAKTAQYQIIDTIQKAY"
BASE COUNT         2932 a         1539 c         1927 g         2577 t
ORIGIN      
        1 ttaactattt agttgtattc caattacttt cactttatta tcactacgat taccattata
       61 tgactagtct ttatcctgaa aatagtcgga gcaagaagaa aaatatgtta agttttttac
      121 gaggtttaag ctttttaatg ttaatcgtca atatgctgtg ttgctcatca gcgccaaaag
      181 cactcatttt gattgtaggt atggttttca tagcttgtat atatctacct tataagttaa
      241 agaagatcat tgactaagta gcctaaaaca agtaaaatag gggtaaacga ctataaaaga
      301 tagaaaagga gggcgataag gtgacaacga cggaacaaga acttaccttg actcccttac
      361 gtgggaaaag tggcaaagct tataaaggca cttatccaaa cggggaatgt gtctttataa
      421 aattaaatac gacccctatt ctacctgcct tagcaaaaga acagattgcg ccacagttac
      481 tttgggccaa acgcatgggc aatggtgata tgatgagtgc ccaagaatgg cttaacggcc
      541 gtacattgac caaagaagat atgaacagta agcaaatcat tcatattcta ttgcgccttc
      601 acaaatctaa aaaattagtc aatcaactgc ttcagctcaa ttataagatt gaaaacccat
      661 acgatttatt ggttgatttt gagcaaaatg cacccttgca aattcagcaa aattcatact
      721 tacaagctat cgttaaagaa ttaaaacgga gcttaccaga gttcaaatca gaagtagcaa
      781 cgattgtgca tggagatatt aaacatagca attgggtgat tactactagt ggtatgattt
      841 ttttagtaga ttgggattct gttcgtctga ctgatcggat gtatgatgtt gcttacctgt
      901 tgagccacta tattccacgg tctcgttggt cagaatggct gtcttattat ggctataaaa
      961 ataatgacaa ggttatgcaa aaaattattt ggtatggtca attttctcac ctgacacaaa
     1021 ttctcaagtg ttttgacaag cgtgacatgg agcatgtgaa tcaggagatt tatgccctca
     1081 gaaaatttag agaaatattt agaaagaaat aatgcgagtt agaaaacgaa aaggtgctga
     1141 ggagcactta gctaataacc cacactatgt gatattaaat ccagaagatg ccaagggccg
     1201 ttggcatgac gtttttggaa atgaccgtcc tattcatatc gaagttggtt caggaaaggg
     1261 cggatttatc acagggatgg ccttgaaaaa tcctgatatt aactacattg gcattgatat
     1321 tcaattatct gtcctaagct acgccttgga taaggtatta gcttcagaag tacccaatgt
     1381 caaacttctg cgagtggatg gatcaagttt aaccaactat tttgaagatg gtgaggtgga
     1441 tatgatgtat ctcaactttt cagatccttg gccaaaaacc aagcatgaaa aacgtcgttt
     1501 gacctacaag gatttcttag acacttacaa gcgtatcttg ccagaacacg gtgaaattca
     1561 ttttaaaacc gataatcggg gcttgtttga gtacagttta gctagttttt ctcaatacgg
     1621 catgaccttg agacagatct ggcttgactt acacgctagt aactatgaag gaaatgtcat
     1681 gacagaatat gaagagaagt tctctaataa aggtcaagtc atttaccgag ttgaggcaaa
     1741 tttctagtag tattttgttc caaaatatga taacataaag gagttgatac agtattaact
     1801 tctttagtaa tggagaggtc gcatagtggc ttagtgcgca cgcttggaaa gcgtgtagtc
     1861 cttaacgggg ctcgggggtt cgaatcccct cctctccttg tgagtaaaac aaagtgcata
     1921 tccttgcatt cctcttaaat tatggtataa taggcttaaa taatacagaa aggtggtggg
     1981 atatgtctcg ccacttttgt attaagtgga ttcccaagga ccaattatct tagagaagag
     2041 cataaaaata gaggaggtaa tcaaaatagc aattacatct attatcgata tcgtaaccaa
     2101 gacagtaaca cctgagatta aggcacctta tgaattagta gacgttgaat atgacaaaat
     2161 gggtagtgac tacatcctta gtattttagt cgataaggaa ggtggaatca cagtggaaga
     2221 tacatctgac ttgacgaata ttattagtcc gctgttagat acgattgatc cagatccttt
     2281 tccaaaccaa tatatgctag aagtctctag tccaggtttg gagcgtccgc ttaagacagc
     2341 tgacagttta aaagcagcag ttggctctta tatcaatgtg agtctttatc aagctattga
     2401 taaggtgaaa gtttttcaag gagacttatt agcctttgat ggtgaaacgt tgaccattga
     2461 ttatcttgat aaaacacgtc ataagatcgt gaatatacca tatcaagcag ttgctaaggt
     2521 gagaatggct gtcaaattat aacttttatt tcgaagcact aaactttttt aggaacgctt
     2581 aagcaaattt gaaacaacac ctatagagtt tatacaagct aaacaaaagg aattaaggga
     2641 gcctttaggg ggctattgat cgtattaatt taagaaagga tttttgccaa gctttacaag
     2701 caaggtagat aacattatga gcaaagaaat gctagaagcc ttccgtattt tggaagaaga
     2761 aaaacacatt gataaagcag atattattga cgcagtgaca gaatcattaa agtctgccta
     2821 taaacgtcgc tatggtcaat cagaatcatg tgttattgaa tttaatgaaa aaacagctga
     2881 ttttcaagta ttcacggttc gtgaggttgt tgaagaagtt tttgacagcc ggcttgagat
     2941 tagtttgaaa gatgctcttg ctatcagttc tgcctatgaa ttaggtgata agattcgctt
     3001 tgaagaatct gtcaatgaat ttggacgtgt cgcagctcaa tcagctaagc agactattat
     3061 ggaaaagatg cgccgccaaa tgcgtgaagt gatgttcaat gaatacaaag agcatgaagg
     3121 tgaaattatg acaggaacag ttgaacggtt tgatcaacgt tttatttatg tcaatcttgg
     3181 ttccttagaa gcacaattgt ctcaccaaga ccagattcca ggtgaaactt tcaaatcaca
     3241 tgaccgtatt gacgtttatg tctacaaagt tgaaaataat cctaaaggag ttaacgtctt
     3301 tgttagtcgt agccatccag aattcatcaa acgtattatg gagcaagaaa ttcctgaagt
     3361 ctttgatggt actgttgaaa tcatgagtgt ctctcgtgaa gcaggtgacc gtaccaaggt
     3421 tgctgtgcgc agtcataatc ctaacgttga tgccatcgga acaattgttg gacgtggtgg
     3481 aagtaatatt aaaaaggtca tcagcaagtt ccatccaaaa cgtgtagatg ctaagacggg
     3541 gcttgaaatt ccagttgaag aaaatattga cgtgattcaa tgggtagatg atccagctga
     3601 gttcatctac aatgccattg ctccggctga agtagacatg gtactttttg acgatgaaga
     3661 tcttaaacgt gctacggtag tagttcctga tagcaaatta tcattagcta tcgggcgtcg
     3721 cggccaaaac gttcgtttag cggcacattt gactggctac cgtattgata tcaaatcagc
     3781 tagcgaatat gatcgtcttg aggcggaaaa agaagcagca acagttgtgg aagagccagt
     3841 tgttgacgat gttatgactg ccgaggaagt gtaagaaaag aggtgtttga tgtctaaagt
     3901 caaaaaaata cctttacgaa aatcacttgt ttctggtgaa attattgcta aacgagatct
     3961 tcttaggatt gtaaaaacta aggatggtca agttttcatt gatccaactg gtaaacaaaa
     4021 tggtcgtggt gcttatatca aattagacaa tcaagaggcc ttaatggcca agaaaaaaca
     4081 agttttcaac aggagtttct caatggacat tccagaaagt ttttacgatg acttgattgc
     4141 ttatgttgat cacaaaatca aaagaagaga gttaggtctt gactaattta gaaagattat
     4201 ctagtcttat tggtctagct caacgggctg gaaaagtgat ttcaggtgaa gaactagtag
     4261 taaaagctat tcaacaccag caggtaatat tagtattttt agccaatgat gctggtccta
     4321 atgtgacaaa aaaagtgaca gataaaagta attattataa tgtagaagtc tccacagtgt
     4381 tgaatgcact ggaattaagt gctgcactag gtaaaccaag gaaggttgct gccattgcag
     4441 atgctggatt ttcaaagaaa atgaggactc ttatggaata gatgaatagg aggacacaaa
     4501 ttgtcaaaga aaagattaca tgaaattgcc aaggaaattg gtaaaagtag taaagaggtg
     4561 gttgaacacg ctaaatactt aggattagat gtgaaaagcc atgcatcaag tgttgaagaa
     4621 gcggatgcga aaaagattat ttcaagtttt tcaaaggctt ctaaacctga tgtgacagct
     4681 tctcaaacag tcaaacccaa agaagttgct caaccctctg taacagtggt caaagaaaca
     4741 ggttctgagc atgtagagaa aacacaggtc tctaagccaa agagtcgtaa ttttaaagct
     4801 gaaagagagg ctcgtgcaaa ggaacaggct gctcgaaaac aagcaaatgg ctcttctcat
     4861 cgttctcaag aacgtcgagg aggttatcgc cagcccaata atcatcaaac taacgaacaa
     4921 ggagataaga ggattaccca tcgttctcaa ggtgatacaa acgataagcg tattgaccga
     4981 aaagcatcga atgtatcacc tagacatgat aatcatcagc tagttggtga tcgtaaccgt
     5041 tcctttgcta aggagaatca caagaatggt cgattcacaa accaaaaaaa gcaaggacga
     5101 caagagcctc aatcgaaaag tcctaaaatt gatttcaaag ctcgtgctgc tgccttaaaa
     5161 gctgaacaaa atgctgaata ctcacgccaa agtgaaacac gttttcgtgc acagcaagaa
     5221 gctaagcgtt tagctgagtt ggcaagacaa gaagctaaag aagcggctct aaaagcacaa
     5281 gctgaggaga tgagccatcg tgaagcagca ctgaaatcta ttgaagaagc agaaactaaa
     5341 ttaaagtcat caaatattag tgctaaatct actgctgata atcgccgtaa aaaacaagca
     5401 cgtcctgaaa agaatcgtga gttaactcat cattcacaag aagggcaaaa gaaaaataag
     5461 aagagttgga atagtcaaaa ccaagtgaga aatcaaaaaa atagtaactg gaataaaaat
     5521 aaaaagacca aaaaaggcaa aaatgttaag aataccaaca ccgctccaaa accagtaacc
     5581 gagcgtaaat tccatgaatt gccaaaagaa tttgaatata ctgaaggcat gacagttgcc
     5641 gaaatcgcaa aacgtatcaa acgcgaaccc gctgaaattg tcaaaaagct gtttatgatg
     5701 ggtgttatgg caactcaaaa ccagtcttta gatggggata ctatcgaatt attgatggta
     5761 gattatggta ttgaggctaa ggctaaggta gaagtagatg atgctgatat cgagcgcttc
     5821 tttgaggatg aaaattacct caatcctgaa aatattgtag aacgcgcacc tgttgtaact
     5881 atcatgggac acgtagacca cggtaagaca actttgcttg atactttgcg taactcacgt
     5941 gtggcaacag gagaagctgg aggaattact cagcatattg gtgcttacca gattgaagaa
     6001 gctggcaaga aaattacctt cttggatact cccggacacg cggcctttac aagcatgcgc
     6061 gcacgtggag cttccgttac cgatatcacc attttgattg ttgcagctga tgatggtgtt
     6121 atgccacaaa cgattgaagc gatcaatcac tcaaaagctg ctggtgttcc aattattgtt
     6181 gctatcaaca aaattgataa accaggtgct aatccagaac gtgttatcgc agaattggca
     6241 gaatatggta tcatctcaac tgcttggggc ggagagtgcg aatttgtaga gatatctgct
     6301 aaatttaata aaaatattga tgaactatta gaaacagtcc ttttggttgc cgaagtggaa
     6361 gaattgaaag ctgatccgac ggttcgtgct attggtactg taattgaagc acgcttagat
     6421 aaaggtaaag gtgctatcgc tacgcttctt gtccaacaag gaactcttca tgttcaagat
     6481 ccaatcgttg ttggtaatac ctttggtcgt gtccgtgcca tggtaaatga ccttgggcgt
     6541 cgtgttaaat ctgcggagcc atcaacacca gtttccatta ctggtttgaa cgaaacacca
     6601 atggctggtg atcactttgc tgtttacgct gatgaaaaag ctgcgcgtgc agctggtgag
     6661 gagcgatcaa aacgtgccct cctcaaacaa cgtcaaaata cacagcgtgt tagccttgat
     6721 aatctctttg atacccttaa agcaggagaa atcaagactg ttaatgtcat catcaaagca
     6781 gacgttcaag gttctgttga agcccttgca gcatctcttg taaagattga agtggaaggt
     6841 gtccgtgtca atgtcgttca ctctgctgta ggtgctatca atgagtctga tgtgaccctt
     6901 gctgaagcat caaatgctgt tattatcggt ttcaacgttc gccctacccc acaggctcgt
     6961 caacaagcag atacggatga tgtggaaatt cgtcttcata gcattatcta taaagtcatt
     7021 gaagaagtag aagaagccat gaaagggaaa cttgaccctg tttaccaaga aaaagttctt
     7081 ggtgaagcta ttattcgtga aaccttcaaa gtttctaaag tgggaacaat tggtggcttt
     7141 atggtaatca acggtaaggt gacacgtgat tcgagtgttc gtgttatccg tgatagtgtc
     7201 gttatttttg atggtaaact ggccagcttg aaacattaca aagatgacgt taaagaagtc
     7261 ggtaatgctc aagaaggtgg gttgatgatt gaaaacttta acgatcttaa agttgatgat
     7321 actattgaag cctatattat ggaagaaatt gtgagaaaat aggggctaag aaggtctgag
     7381 atgtgacgtt tttcctctgt cgaaaataat gactttttct accagatgag ctagttgggg
     7441 cattccttcc agtctcgtca tggaattctc agattccctt taattgtcgt tatcaaaaca
     7501 ggtagcaatg tcataagctg ggctttcagc ctagcttttg ttttaaaaat gtggaaagga
     7561 aatggctatg gctaatcatc gtattgaccg cgttgggatg gaaatcaagc gcgaagtaaa
     7621 tgatattctt cagaaaaaag tgcgtgatcc acgcgttcag ggagtaacga ttacagaagt
     7681 gcaaatgcag ggtgacttgt cacttgctaa agtttattac actattatga gtgacttggc
     7741 atctgataat caaaaagcac aaacagggct tgaaaaagcg actggaacga ttaaacgtga
     7801 acttggcaaa caattgacca tgtacaaaat tccagatttg gtgtttgaaa aagacaattc
     7861 catcgcctat ggtaataaaa ttgatcaact cttacgtgag cttgataaaa aagattaaat
     7921 taaaaacctt taaggtatct tattagagtc accttaaggg ttttttgttt ttagtaagct
     7981 ttttgaatcg tatcaatgat ttgatattgt gcagtcttgg cttcttcaat tggatagatt
     8041 gggtaaacat gattcatttt atcagcgacg ataagttcat gttgatgacc tttggcagtt
     8101 aatatatcat caaatttctt aacatctggg tagaaaattt catgtgtacc aacaaacata
     8161 gtaataggtg ctagccctga tacatctcca ttaattggac taacatatgg atgtttggtg
     8221 ttgtttgggt cacggcgtgc ccaaatttcc ccaactttac gtaggcccca agctgaaaga
     8281 atgggatcat gactttcata gctctcaata tctggatttg acatggttac atctaaccat
     8341 ggtgataaga gaacaatgtc ttttggttgt tttaaacttt cattttttag ggctaatgct
     8401 aaaccaagcg ctagtccacc acctgctgaa tcacccataa tagtgatatt ttgtgcagtt
     8461 gtttctttaa gagtatctct gtaaaggtca accaattttg gcatagcatc actgtaagag
     8521 taagtaggtg cttttggata gattggtaag ataatcttag catcaagact agttgataag
     8581 gtttttagca tgtcgatatg ataagtagtt gggttgttta ggtaagagcc cccatgtaaa
     8641 tagaaaatga ccttttgatt ggctttattg ctggggttct cattccaagt atgaacttgc
     8701 atattatcaa cagttttagt gactaatgtg ccatttacct gttcttcttt aagatcaaag
     8761 cctttatttg aagcttctcc agaagagttg acatattgat caaacttagt ttcgtcttgc
     8821 tctgaataat agcctttgtc tccttttaac caaaagtatt tctctataaa ccagcttttc
     8881 caagatctag aactggcatc aacttgagtg ctagctccta ttgctagagt aagtcctagt
     8941 aaaataagag ggcttttaag tgtctttttc aatga
//
LOCUS       DAUELZ010000067         8888 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.068, whole genome shotgun sequence.
ACCESSION   DAUELZ010000067 DAUELZ010000000
VERSION     DAUELZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.068"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            4..1605
                     /locus_tag="VHI09_001226"
     CDS             4..1605
                     /locus_tag="VHI09_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ6121102.1"
                     /translation="MHVSKLFTSQQWRELKLISYLTENSNAIGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHQCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGDERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKNCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYSCSYNNKKTSHRFSQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMLSNHLNDKLE
                     IGCAFEIINQDPTSGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDI
                     TANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFIT
                     WENSFLKVNQKDEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIE
                     KQYDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSS
                     SKSHSDKEVQKPEKPDNSVKQATSS"
     gene            1794..2960
                     /gene="mrp"
                     /locus_tag="VHI09_001227"
     CDS             1794..2960
                     /gene="mrp"
                     /locus_tag="VHI09_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-related protein Mrp"
                     /protein_id="HEQ6121103.1"
                     /translation="MSKRNPNKLYSLRKLKTGTASVAVALTVLGTGLANTTDVKAESR
                     RYQAPPRVLLQGKEANKVFEERKALEKQARDLGDTINHMSQTISEQSRKIAALKSEAE
                     LKNQQALEALNNKNKQISDLTNENAQLKEAIEGYVQTIQNASREIAAKQQELAAAKSQ
                     LEAKNAEIEALKQQDASKTEEIAKLQSEAATLENLLGSAKRELTELQAKLDTATAEKA
                     KLESQVTTLENLLGSAKRELTDLQAKLDAANAEKEKLQSQAATLEKQLEATKKELADL
                     QAKLAATNQEKEKLEAEAKALKEQLAKQAEELAKLKADKASGAQKPDTKPGNKEVPTR
                     PSQTRTNTNKAPMAQTKRQLPSTGEETTNPFFTAAALTVIASAGVLALKRKEEN"
     gene            3187..4365
                     /locus_tag="VHI09_001228"
     CDS             3187..4365
                     /locus_tag="VHI09_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ6121104.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFANQTEVKAAEI
                     KKPQADSAWNWPKEYNALLKENEELKVEREKYLSYADDKEKDPQYRALMGENQDLRKR
                     EGQYQDKIEELEKERKEKQERQEQLERQYQIEADKHYQEQQKKHQQEQQQLEAEKQKL
                     AKDKQISDASRQGLSRDLEASRAAKKELEAKHQKLEAENKKLTEANQVSEASRKGLSN
                     DLEASRAAKKELEAKHQKLEADYQVSETSRKGLSRDLEASREANKKVTSELTQAKAQL
                     SALEESKKLSEKEKAELQAKLDAQGKALKEQLAKQTEELAKLRAEKAAGSKTPATKPA
                     NKERSGRAAQTATRPSQNKGMRSQLPSTGEAANPFFTAAAATVMVSAGMLALKRKEEN
                     "
     gene            4701..8198
                     /gene="scpA/B"
                     /locus_tag="VHI09_001229"
     CDS             4701..8198
                     /gene="scpA/B"
                     /locus_tag="VHI09_001229"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEQ6121105.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VEAPQQTAVSEEAPSSSKETNPPQTPDDAEETVADKANDLAPQAPAKTADIPATSKET
                     IRDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKSKARYQSKEDLEKA
                     KKDHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRIEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTADKQDKEMPVLSTNRFEPNKAYDYAYANRGMKEDDFKDVKGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     SKKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMK
                     NGYFLEGFVRFKQDPKKEELMSIPYIGFRGDFGNLSTLEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPIYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSNKPEQDGSDQAPDKKPETKPEQDGSGQTPDKKPEAKPEQDGSGQTPDKKPETKPE
                     KDSSGQTPGKTPQKGQPSRTLEKRSSKRALATKASTKDQLPTTNDKDTNRLHLLKLVM
                     TTFFLGLVAHIFKTKRTED"
     gene            8295..>8888
                     /locus_tag="VHI09_001230"
     CDS             8295..>8888
                     /locus_tag="VHI09_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ6121106.1"
                     /translation="MFRVENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDSIPPI
                     SLTQRTPATTSEKWHHIDDKGSIPLGISLEAAKKDFKEEVEKSRLSEEQKQTYNQKID
                     AETDKDELLSTYHRDYLTAVKNLPTSTEPVEASVQETQASVSDSMVTGDSTSVTADSP
                     KESPVAPASSPESEDSSVASSEETSSPETPVAPETPEE"
BASE COUNT         3261 a         1766 c         1744 g         2117 t
ORIGIN      
        1 ttaatgcatg taagtaaatt gtttactagc caacaatgga gagaattgaa actgatttca
       61 tatttaacag aaaactctaa tgctatcgga gttaaagata aggagcttag caaggctctc
      121 aatatttcca tgttaacatt acagtcatgc ctcactaata tgcagttcat gaaagaagta
      181 ggtggaatta cctacaaaga cggctatatt aatatttggt atcaccagtg ttgtgggcta
      241 caagaggtct atcaaaaagc tcttcgagaa tcgccttctt tgaaattact agagcttttg
      301 tttttcagag actttagttc cttagaagag ttagctgaag agctgtttgt cagcttatct
      361 accctcaaac gcctcatcaa aaaaaccaac acttacttgt ctcatacctt tgctattagt
      421 atcgtgacaa gtccagtgca ggtgtcagga gatgagaggc aaatccgcct gttttacctc
      481 aagtattttt cagaagccta taaaatctca gaatggccat ttggggacat tttaaacctg
      541 aaaaactgtg aacgcctatt gagcttattg atcaaagaag tagatgtcaa agttcatttt
      601 acactatttc agcacttaaa aatcttaagt ggtgtcaact tgatccgtta ctacaagggg
      661 tattcttgca gttataacaa taaaaaaacc agtcaccgtt tttctcaact tatccaacat
      721 tcctcagaaa tccaggatct ttctcgcttg ttctacctca aatttgggtt acatctcgac
      781 gagtatacca tagctgaaat gctttctaac catcttaacg acaagctaga aattggctgt
      841 gcttttgaga ttatcaatca agacccgaca tcaggaggca gacaagtaac caactggatc
      901 catctattag atgagatgga aatcaagctc aatctcagca tcaccaataa atacgaagtt
      961 gccgtgaccc tccataacgc ttcggtgtta aacgaagaag acattacagc caattatttg
     1021 ctgtttgatt ataaaaagag ttacctgaac ttttaccaaa aagaacaccc tcgcatttat
     1081 gaggcctttg tgacaagtgt cgagaagctg atgcaggcag ataatgctca agttagcaaa
     1141 gaactgatca atcagctcac ttattgtttc tttattacct gggaaaatag tttcttaaaa
     1201 gtaaatcaaa aagatgaaaa agtacgactc ctagtgatag agagaagtta caatagtgtc
     1261 ggtaattttt tgaaaaagta catcggtgag ttttttagca tcacgaactt tgatgagtta
     1321 gattgtttga caattgatct agtagagatt gaaaaacagt acgatgttat cgtgacagat
     1381 gttatggtgg gtaaaagcga agagttagag attttctttt tctacaagat gattccagaa
     1441 gctattattg atagattaaa cgaatttttg aacgttagtt tcacagataa caacgttatg
     1501 gtcaaaaacc tcgaagcccc ttcctcttca aaatctcata gtgacaaaga ggtacaaaag
     1561 ccagaaaagc cagacaattc agttaaacaa gcaacatcat cataggattt cagacgtcat
     1621 ggtaagaaaa aaaagaatca tttacagccc atagaggtaa ggtcaaaagc tgaaaacagc
     1681 tcaaaaaaac tgacctttac cttttggctt ttttttattt agaataattt tattggagag
     1741 atgcttaata atttaagcac aattcttaga aattgagaaa taaggagtaa acaatgtcta
     1801 aaagaaatcc aaacaaactc tattcactga gaaagttaaa aacaggtact gcatcagtag
     1861 cagtagcctt gacagttttg gggactggat tagcaaacac aactgatgta aaggctgaga
     1921 gtcgtcgtta tcaggcacct cctcgtgtgt tactgcaagg caaagaagct aacaaagtat
     1981 tcgaagagcg caaagccttg gaaaaacaag cacgtgattt gggtgacact attaaccaca
     2041 tgtcacaaac cattagcgag caaagccgca agattgcagc actaaagtct gaagcagaac
     2101 ttaaaaacca acaagctctt gaagctttaa acaataaaaa caagcaaatc tcagatttaa
     2161 ccaacgaaaa cgcacagtta aaagaagcca ttgaaggtta tgtgcaaact atccaaaacg
     2221 ctagtcgtga aatcgcagca aaacaacaag aacttgcagc tgcaaaaagc cagttagagg
     2281 caaaaaatgc tgagattgag gcattgaaac aacaagatgc ctctaagact gaggaaattg
     2341 ctaaattgca atcagaagca gcaactttag aaaacctcct aggttcagct aagcgtgagt
     2401 tgactgaatt gcaagctaag ctagatacag caactgctga aaaagcaaaa ctagaatcac
     2461 aagtaacaac cttagaaaac ctcctaggtt cagctaagcg tgaattgact gatctgcaag
     2521 ctaagctaga tgcagctaac gctgaaaaag aaaagctcca atcacaagca gcaaccctag
     2581 aaaaacaact agaagcaact aaaaaagagt tagctgattt acaggctaaa ttagcagcaa
     2641 ccaaccaaga aaaagaaaag ttagaagctg aagcaaaagc tcttaaagag caattggcta
     2701 aacaagctga agagcttgct aagctaaaag cagataaagc ttcaggagct caaaaaccag
     2761 atactaaacc tggcaataaa gaggttccaa caagaccgtc acaaacaaga acaaacacta
     2821 ataaagctcc tatggctcaa acaaagagac aattaccgtc aacaggcgaa gaaacaacca
     2881 acccattctt cactgcagca gcattgacag tgatcgcatc tgcaggtgta cttgccctaa
     2941 aacgcaaaga agaaaactaa gtccaaccca cattatcttt tctagcccaa gaaaaaaaca
     3001 aaaaaagagg aagccccttc ctcttttttt gaacggttaa acagcaaaaa ggtcaaaaag
     3061 gcactaaagt ctcaaaaacc tggtctttac cttttaccgc tcattcttta gaatagaatt
     3121 attagagaga agtcttagaa aaatgaggct aattccctaa aagatgaaaa aaataaggag
     3181 caaataatgg ctagaaaaga tacgaataaa cagtattcgc ttagaaaatt aaaaacaggt
     3241 acagcatcag tagcggtcgc tgtggctgtt ttaggagcag gctttgcaaa ccaaacagaa
     3301 gttaaggctg cggagattaa aaagcctcag gctgattcag cgtggaactg gcctaaagaa
     3361 tataacgcgt tacttaagga aaatgaggag ctcaaggtag aacgtgaaaa atatctatct
     3421 tatgctgacg ataaagaaaa agatcctcaa tatagagcat taatgggtga aaatcaagat
     3481 cttcgaaaaa gagagggaca atatcaggac aaaatagaag aacttgaaaa agaaagaaaa
     3541 gaaaaacaag aaagacaaga acaattagaa cgtcaatatc aaatagaagc agataagcat
     3601 tatcaagaac aacaaaagaa acatcagcaa gaacaacaac aattagaagc agaaaaacaa
     3661 aaattagcta aagacaaaca aatctcagac gcaagccgtc aaggcctaag ccgtgacctt
     3721 gaagcttctc gagcagctaa aaaagaacta gaagctaagc accaaaaatt agaggctgaa
     3781 aacaaaaaac taacagaagc caatcaggtt tcagaagcta gtcgtaaagg tctaagtaac
     3841 gacttagaag cttctcgagc agctaaaaaa gaactagaag ctaagcacca aaaattagag
     3901 gctgattacc aagtttcaga gactagccgt aagggtctaa gccgtgacct tgaagcatca
     3961 cgtgaagcta ataagaaggt tacatctgag ttaacacaag caaaagctca actctcagcg
     4021 cttgaagaaa gtaagaaatt atcagaaaaa gaaaaagctg agttacaagc aaaactagat
     4081 gcacaaggaa aagccctcaa agaacaatta gcaaaacaaa ctgaagagct tgcaaaacta
     4141 agagctgaaa aagcagcagg ttcaaaaaca cctgctacca aaccagctaa taaagaaaga
     4201 tcaggtagag ctgctcaaac agctacaaga cctagccaaa ataaaggaat gaggtcacaa
     4261 ttaccgtcaa caggcgaagc agccaaccca ttctttacag cagcagctgc aacagtgatg
     4321 gtatctgcag gtatgcttgc tctaaaacgc aaagaagaaa actaagcctt tagaacttga
     4381 tttttgtaac ggtgcaatag acaaaagcaa gcaaggccaa aaactgagaa agtcctaaaa
     4441 agctggcctt tacccctcaa aattaatgtt ttataataaa gatgttagta atataattga
     4501 taaatgagat acatttaatc attatggcaa aagcaagaaa aatagctgta tcatatgcaa
     4561 ataacccctg tttgctcttt aaaaaaagac gttatcctta tttctctacg cacagatgga
     4621 cagctaggag agaatcgttt gattctctct tttcttaatg gtcataaaga caaagtctct
     4681 catcaggaaa ggacgacaca ttgcgtaaaa aacaaaaact accatttgat aaacttgcca
     4741 ttgcgctcat gtctacgagc atcttgctca atgcacaatc agacattaaa gcaaatactg
     4801 tgacagaaga cactcctgct accgagcaag ccgtagaagc cccacaacaa acagcggttt
     4861 ctgaggaagc accatcatca tcaaaggaaa ctaacccccc acaaactcct gatgacgcag
     4921 aagaaacagt agcagataag gctaatgatc tagcccctca agctcctgct aaaactgctg
     4981 atataccagc aacctcaaaa gagactatta gggatttgaa cgacccttct catgtcaaaa
     5041 ccctgcagga aaaagcaggc aagggagctg ggactgttgt tgcagtgatt gatgctggtt
     5101 ttgataaaaa tcatgaagcg tggcgcttaa cagacaaatc taaagcacgt taccaatcaa
     5161 aagaagatct tgaaaaagcc aaaaaagatc acggtattac ctatggtgag tgggtcaatg
     5221 ataaggttgc ttattaccac gactatagta aagatggtaa aaccgctgtc gatcaagagc
     5281 acggcacaca cgtgtcaggg atcttgtcag gaaatgctcc atctgaaacg aaagaacctt
     5341 accgcataga aggtgcgatg cctgaggctc aattgctttt gatgcgtgtc gaaattgtaa
     5401 atggactagc agactatgct cgtaactacg ctcaagctat cagagatgct gtcaacttgg
     5461 gagctaaggt gattaatatg agctttggta atgctgcact agcttacgcc aaccttccag
     5521 acgaaactaa aaaagccttt gattatgcca aatcaaaagg tgttagcatt gtgacctcag
     5581 ctggtaatga tagtagcttt gggggcaaga cccgtctacc tctagcagat catcctgatt
     5641 atggggtggt tgggacacct gcagcggcag actcaacatt gacagttgct tcttacagcc
     5701 cagataaaca gctcactgaa actgctacgg tcaaaacagc cgataagcaa gataaagaaa
     5761 tgcctgttct ttcaacaaac cgttttgagc caaacaaggc ttacgactat gcttatgcta
     5821 atcgtgggat gaaagaagat gattttaagg atgtcaaagg taagattgcc cttattgaac
     5881 gtggcgatat tgatttcaaa gataagattg caaacgctaa aaaagctggt gctgtaggag
     5941 tcttgatcta tgacaatcag gacaagggct tcccgattga attgccgaat gttgaccaga
     6001 tgcctgcggc ctttatcagt cgaaaagacg gtctcttatt aaaagacaat tctaaaaaaa
     6061 ccatcacctt caatgcgaca cctaaggtat tgccaacagc aagtggcacc aaactaagcc
     6121 gcttctcaag ctggggtctg acagctgacg gcaatattaa gccagatatt gcagcacccg
     6181 gccaagatat tttgtcatca gtggctaaca acaagtatgc caaactttct ggaactagta
     6241 tgtctgcgcc attggtagcg ggtatcatgg gactgttgca aaagcaatat gagacacagt
     6301 atcctgatat gacaccatca gagcgtcttg atttagctaa aaaagtattg atgagctcag
     6361 caactgcctt atatgatgaa gatgaaaaag cttatttttc tcctcgccaa caaggagcag
     6421 gagcagtcga tgctaaaaaa gcttcagcag caacgatgta tgtgacagat aaggacaata
     6481 cctcaagcaa ggttcacctg aacaatgttt ctgataaatt tgaagtaaca gtaacagttc
     6541 acaacaaatc tgataaacct caagagttgt attaccaagc aactgttcaa acagataaag
     6601 tagatggaaa acactttgcc ttggctccta aagcattgta tgagacatca tggcaaaaaa
     6661 tcacaattcc agccaatagc agcaaacaag tcaccgttcc aatcgatgct agtcgattta
     6721 gcaaggactt gcttgcccaa atgaaaaatg gctatttctt agaaggtttt gttcgtttca
     6781 aacaagatcc taaaaaagaa gagcttatga gcattccata tattggtttc cgaggtgatt
     6841 ttggcaatct gtcaacctta gaaaaaccaa tctatgatag caaagacggt agcagctact
     6901 atcatgaagc aaatagtgat gccaaagacc aattagatgg tgacggatta cagttttacg
     6961 ctctgaaaaa taactttaca gcacttacca cagagtctaa cccatggacg attattaaag
     7021 ctgtcaaaga aggggttgaa aacatagagg atatcgaatc ttcagagatc acagaaacca
     7081 tttttgcagg tacttttgca aaacaagacg atgatagtca ctactatatc caccgtcacg
     7141 ctaatggcaa gccatatgct gcgatctctc caaatgggga cggtaacaga gattatgtcc
     7201 aattccaagg tactttcttg cgtaatgcta aaaaccttgt ggctgaagtc ttggacaaag
     7261 aaggaaatgt tgtttggaca agtgaggtaa ccgagcaagt tgttaaaaac tacaacaatg
     7321 acttggcaag cacacttggt tcaacccgtt ttgaaaaaac gcgttgggac ggtaaagata
     7381 aagacggcaa agttgttgct aacggaacat acacctatcg tgttcgctac actccgatta
     7441 gctcaggtgc aaaagaacaa cacactgatt ttgatgtgat tgtagacaat acgacacctg
     7501 aagtcgcaac atcggcaaca ttctcaacag aagatcgtcg tttgacactt gcatctaaac
     7561 caaaaaccag ccaaccgatt taccgtgagc gtattgctta cacttatatg gatgaggatc
     7621 tgccaacaac agagtatatt tctccaaatg aagatggtac ctttactctt cctgaagagg
     7681 ctgaaacaat ggaaggtgct actgttccgt tgaaaatgtc agactttact tatgttgttg
     7741 aagatatggc tggtaacatc acttatacac cagtgactaa gctattggag ggccactcta
     7801 ataagccaga acaagacggt tcagatcaag caccagacaa aaaaccagaa actaaaccag
     7861 aacaagacgg ttcaggtcaa acaccagaca aaaaaccaga agctaaacca gaacaagacg
     7921 gttcaggtca aacaccagat aaaaaaccag aaactaaacc agaaaaagat agttcaggtc
     7981 aaacaccagg taaaactcct caaaaaggtc aaccttctcg tactctagag aaacgatctt
     8041 ctaagcgtgc tttagctaca aaagcatcaa caaaagatca gttaccaacg actaatgaca
     8101 aggatacaaa tcgtttacat ctccttaagt tagttatgac cactttcttc ttgggattag
     8161 tagctcatat ctttaaaaca aaacgcactg aagattagta caactgatca gatggctact
     8221 attgatgatg agttagatca gtcttctttt caaaagaagg catagcagta taaaaataga
     8281 tatgttagga caatatgttt agagtagaaa ataacaaaca cagccgctat tccattcgca
     8341 aactgagcgt tggggtaacg agtatagcaa ttgcgagtct ctttttagga aaggttgcct
     8401 atgccgtaga tagcatccct ccaatctctc ttactcaaag gactccagcc actacatcag
     8461 aaaaatggca tcatattgat gataagggct ctattccttt aggtataagc ttagaagctg
     8521 ccaaaaaaga ttttaaagaa gaagtagaaa aatcacgttt gtctgaagaa caaaaacaaa
     8581 cgtataatca aaaaattgat gctgaaacag acaaagacga actattgtca acgtatcata
     8641 gagactatct gacagccgtt aagaatcttc caacgtctac tgagccagtt gaggcatccg
     8701 tgcaggagac acaggcatca gtttcagatt cgatggtgac aggtgattca acatcagtta
     8761 cggctgattc tcctaaggaa agtccagtag ccccagcttc atcacctgag agtgaggatt
     8821 cttcagtagc atcttctgag gaaacctcat ctccagaaac tccagtggcc ccagaaactc
     8881 ctgaagag
//
LOCUS       DAUELZ010000068         8865 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.069, whole genome shotgun sequence.
ACCESSION   DAUELZ010000068 DAUELZ010000000
VERSION     DAUELZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.069"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            408..1475
                     /gene="pepA"
                     /locus_tag="VHI09_001231"
     CDS             408..1475
                     /gene="pepA"
                     /locus_tag="VHI09_001231"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEQ6121107.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPNIENIVFDGGFTDKAEAESFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEDAGVNFQYYCGKGGTDAGAAHLQNDGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKRY"
     gene            1523..2293
                     /locus_tag="VHI09_001232"
     CDS             1523..2293
                     /locus_tag="VHI09_001232"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEQ6121108.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLT
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(2376..2684)
                     /locus_tag="VHI09_001233"
     CDS             complement(2376..2684)
                     /locus_tag="VHI09_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121109.1"
                     /translation="MYVFIESNDLKDYLITVYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLPLIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(2871..4067)
                     /locus_tag="VHI09_001234"
     CDS             complement(2871..4067)
                     /locus_tag="VHI09_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEQ6121110.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEAVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GQSADTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGDISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            complement(4126..5079)
                     /locus_tag="VHI09_001235"
     CDS             complement(4126..5079)
                     /locus_tag="VHI09_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ6121111.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLVPVNLLTSPQSQLLKQWLKDYAQVVTLITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            complement(5177..5503)
                     /locus_tag="VHI09_001236"
     CDS             complement(5177..5503)
                     /locus_tag="VHI09_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ6121112.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            complement(5487..5921)
                     /locus_tag="VHI09_001237"
     CDS             complement(5487..5921)
                     /locus_tag="VHI09_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEQ6121113.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            complement(5914..6150)
                     /locus_tag="VHI09_001238"
     CDS             complement(5914..6150)
                     /locus_tag="VHI09_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ6121114.1"
                     /translation="MIATGILFSIVILILSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEMTLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            complement(6155..6583)
                     /locus_tag="VHI09_001239"
     CDS             complement(6155..6583)
                     /locus_tag="VHI09_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEQ6121115.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            complement(6558..6884)
                     /locus_tag="VHI09_001240"
     CDS             complement(6558..6884)
                     /locus_tag="VHI09_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEQ6121116.1"
                     /translation="MINQWNNLRHKNLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            complement(6886..7872)
                     /locus_tag="VHI09_001241"
     CDS             complement(6886..7872)
                     /locus_tag="VHI09_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEQ6121117.1"
                     /translation="MKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYV
                     LKMEESLLKGQGLADMLSGLGFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRR
                     KTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLNHFPAFFIGFCSGLILL
                     FGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDI
                     MAIEKSSLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELE
                     IYAQESWEQFFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILLPIYQNMGGIF"
     gene            complement(7856..8794)
                     /gene="tadA"
                     /locus_tag="VHI09_001242"
     CDS             complement(7856..8794)
                     /gene="tadA"
                     /locus_tag="VHI09_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEQ6121118.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYARLLELGITKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
BASE COUNT         2764 a         1836 c         1544 g         2721 t
ORIGIN      
        1 gatttctact gttatgtatt gacaaaaata gtaaaaacaa ctaaactaaa ggtacgttga
       61 ttaggtgtgg tttattatta atttaaccac gctataaaaa tcactgatta acctaaaatg
      121 gaaggttctc cttatgactc cattatcctt acttttcgct agtgaagagt cttttcgcat
      181 cctaagcgcc ctactattct tattaaccag cttgtgcttt atcattttaa tgatttcctt
      241 gctgcctaaa aaaacaggtt atcgccaaaa gacaaacact tcttccagag atctctagtc
      301 tctgggattt ttctttgctg tgaaacccat tcttacttaa aaagtggtag aatataggga
      361 gatgaccgcg ttgaactggt ctgaaacctt gtaaatggag atgaaaaatg acagacttat
      421 tctcaaaaat caaagaagtt accgaactgg atggcattgc gggctatgaa catagcgttc
      481 gtgactacct acgcaccaaa ataaccccgc tggttgaccg tgttgaaaca gacgggcttg
      541 gtggcatttt tggtatcaga gatagtaaag ctgaaaaagc cccccgtatt ttagtagctg
      601 cgcacatgga cgaagtcggt tttatggtca gtgatatcaa agttgacgga acgctacgcg
      661 tggttggtat cggtggttgg aacccacttg ttgtcagttc acaacggttt accctttaca
      721 cacgcactgg ccaagttatt ccccttattt caggatcggt acctccccat tttttacgtg
      781 gggcaaatgg ctctgctagt ctaccaaata tcgaaaatat tgtgtttgat ggtggcttta
      841 cggataaggc agaagctgaa agctttggca ttacaccggg tgatattatt gtccctcaat
      901 ctgaaacgat cctaacagcc aatcaaaaaa atattatttc aaaagcttgg gacaatcgct
      961 atggcgttct catgataaca gaaatgcttg aagcgttaaa aggacaagac cttaacaaca
     1021 ccctaattgc aggtgctaac gttcaagaag aagttggtct gcgcggagcc cacgtctcaa
     1081 ccaccaagtt cgaccctgaa ctctttttcg cagtagattg ttcgcctgct ggtgatattt
     1141 atggcaatcc tggaacaatc ggagatggca ccttgttgcg tttctacgac ccaggccatg
     1201 tcatgctcaa agatatgcgc gacttcttac tgactactgc tgaggacgct ggtgtcaatt
     1261 tccaatacta ttgtggaaag ggaggcacag atgcaggtgc tgcacacctt caaaatgatg
     1321 gtgtcccatc aacaactatc ggagtctgtg cacgctacat tcactctcat caaaccctct
     1381 acgctatgga tgatttcgta gaagcccaag ccttcttaca agccattatc aaaaaactgg
     1441 atcgctcaac cgttgacttg attaaacgtt actaaactag cgttagcctg attaaccata
     1501 atattccaaa aggagcaaca gaatgaaaat tggcattatt ggtgttggca aaatggctag
     1561 cgctatcatc aaaggcctta aacaaacacc ccatgaactt atcatttcag gatcatcttt
     1621 agaacggtcc aaggaaattg cggagcagtt aacactgcct tatgctatgt cccaccaaga
     1681 ccttattgac caggttgatc ttgttatttt aggcatcaag cctcaactat ttgaaacggt
     1741 actcaaaccg cttcacttca aacagcctat tatatctatg gcagcaggca tttcccttca
     1801 acgactagca acattcgtag gacaagacct tccgctgctg cgtatcatgc caaacatgaa
     1861 tgcacaaatt ctccaaagca gtaccgcttt aacgggaaat gctttggtgt cccaggaatt
     1921 acaagcacgt gttcgagact taacagatag ctttggtagc acatttgata ttagtgaaaa
     1981 ggattttgac acctttaccg ctttagcagg gtcaagtcct gcctatattt atctctttat
     2041 tgaggctttg gctaaggctg gcgtcaagaa tggcatgcct aaagcaaagg cgctggagat
     2101 tgttactcaa acagtcttgg ctagtgccag caatctcaag accagttctc aaagtccgca
     2161 cgatttcatt gacgctattt gtagccccgg tggcacaact attgctggtc tgatggagtt
     2221 agaacgcctt ggcctcacaa ctactgtcag ctctgccatt gacaaaacca tcgataaggc
     2281 taaaagcttg taaaataagt gccccaatac aaatcagaca acaatacaaa cacctccgtt
     2341 gaccagacag gaaactcaac cgttagacaa gtcatctaac aaagaacctg actcgtatca
     2401 aacggaggct tttgactatt agttgttatt ttccctagtg cctccatttt cttagccacc
     2461 agacttctga taatcaagta aaggtaagca aaaatcaaag gtagcttata agaaacaagg
     2521 tctcgaatgg ttaagcaagg gaagaaaaag aacaaggcaa caaatagaac aatcatcagg
     2581 taaaagcagt aaacttgtgt ctgttttcga atcgcacgat tacgctcatc ataaaccgtt
     2641 atcaaatagt cttttagatc attggactct ataaacacat acaagaaaag cgttgaagca
     2701 attaaaaact tctaaataag gctgacatcc aaatcgccta gcacaagatt acaaaaagca
     2761 aggagactga tcagaactaa tacctaggca ccataaccaa cagataaacc tcataaaaaa
     2821 ccaagtaaag tgttacctta cttggttaat ctattgagac ttagcctctt ttattttgta
     2881 tttttcaaac gctcaacgtc acgcgcgatg cacaattctt cgtctgttga gataacaaga
     2941 accttcactt ttgactcagg tgttgaaata tcaccacggt atccaaaaac atttttctca
     3001 ggatcaatgt ccattccgaa ccacgttaag ccaccaataa cgtcttgacg catcaatggt
     3061 gcattctcac ccataccagc tgtaaagacc aaagcatctg ccccattcaa aacagcaaag
     3121 tattgaccaa tgcatttttt aatgcgatca ataaagatat tgtaagctaa aacagcatct
     3181 ggattatctt cttgtaaacc agcctcgata tcacgcatat cactagaaat gcctgacacc
     3241 ccactaagac ctgatttctt atttaacata ttcacaacat cagcagcatc ttttaattct
     3301 gggtcttgct caattaaata aggaataata gctgggtcaa tgtcaccaga acgtgttccc
     3361 atcataggac cagcaagtgg tgtaaagccc atcgaagtat cagctgattg accatgatag
     3421 ttagccgtaa ttgacacacc gtttccgatg tgagcagtga ttaatttcaa ttcttctaat
     3481 ggacgaccaa gcattttagc agcttcttgc gccacatatt tatgacttgt cccatgcgcc
     3541 ccatatttac gaaccttgta gtccgtataa tatttttgag gaattgggta aaggtaagta
     3601 tgctttgcca tgctggtgtg aaatgacgtg tcaaacacac aaacactggt aatatctggc
     3661 aaaatatcac ggaaggcacg aatccctgcc gccgctccag gattgtgaag tggagctaaa
     3721 acggataatt cttcaatctg ttcaagaact ttatcattaa ccacaacgga ttctttgaaa
     3781 agttcaccac cagcaacaac acggtgtcca actcctgtaa tttcatcata agctgcaatg
     3841 attccaaagt gaatcaaatc gttcaataag attttaacag cttctgtatg atcgtgaata
     3901 tcaagaattt gttcttcttt tttgccatcg tacttaactg ttgaaataga atccttgaga
     3961 ccgatacgct caataattcc ttgtgctaac actgcttctt ctggcatctg ataaagttgc
     4021 cattttaaac tagaactacc tgcattaatt gcaattgtct ttgacatttt taacctctct
     4081 tgaaatcgtt ttctgtgatg cttttttatt ataacaaaaa atcaattaat tgacattact
     4141 cagtttccat tttttgaaat tttccataaa atcatgaata ttctctgcaa gcttcaaatc
     4201 ccgaattgga tagacaaagg tttccattgg gtggtctgtt tgtttttgta agacaataat
     4261 ggacttggca tttgagggat gaccaaaaat agaatctggt agcgtaatca aggtcaccac
     4321 ctgagcataa tcttttaacc actgtttcaa taactggctc tgagggcttg tcaataaatt
     4381 gactggtacc agaaaaatcg caaaaccgtc ttttttcagg tattttaaag actgttccat
     4441 taataaatga tgggcatagg tatgcttatc tgaactagcc accttgtacc gtttagcaat
     4501 atcatcgtta ggataataac caactggtaa gtcactgatg acaatgtcac tttcttttag
     4561 taattgaggc cttaccgcat cttcttgaat aaaatgagct gaagaatcca ttatctcagc
     4621 aatactggct gacagatcaa tcaagagatc atcaagttca atccctacat aatcaaggct
     4681 cttgctcgtg ttgttgagaa gggtttgggc taggttccct gttccacttc caatctcaag
     4741 tacctctaag ctatctttat cactcaattg ttccaaaaga tacagcagaa tgaagccaat
     4801 agcatctggt gtgaactgat ggttggcttg gagttgctcc gtctgagctg ccttaataaa
     4861 aagaaactgg taggccttac gccattcttc ttttgtcagg cacaaggctt tcagtttatc
     4921 actattttga gcaacttgag gactagctcc ctcagcccct aaataaaagg agttttgttc
     4981 aacaatggca tcgtatatat gtgtttttag atcattttca attagttgac agttctctaa
     5041 gagcagctga taagcttctt caattttttc aaaagtcata accttatcat accaaattac
     5101 acttgaaatg aaaagaagtg tcacctttaa ttactatata ttggatcatt ttaaggattt
     5161 atgacaacaa tactatttat tgactatatt gtttgggttt aaggtattca aactgatact
     5221 gtcgttgctg gtctaactta acaattaaca tccatctctc ttttcgctct cgacaaatga
     5281 cttgaccacc tttaaaaaag acaggattag aagctttata tctatgatct tgataaacta
     5341 tttctgctat cagtcgggct ttaacatagt cagtttgagc ttggtattcc ttgtgtagag
     5401 ctgtcacttg agaaaggtaa acctgtaaaa ccagcaaaaa aatactagcc ataaacatag
     5461 catacaaaag gatgcctgct ttgagattag tcttgaaagg cataataaaa agtcctttct
     5521 aacccacttt tccactttaa agtaatgcaa atctgtgact gctctatgtg aatatgacta
     5581 cgtgatattc caaataacat gggttgatac ccttttccat tactagctga ttttcgaaaa
     5641 tcatgacttt taaattggcc aaaagctagt actttctttc ccttttcaac gtatagttta
     5701 ttatcagcta ctttgtaaaa tcttgctcca cttaactcct ctcgcaattg atgagaaaat
     5761 aagagccaat tgtcttgatc atgacgggct agataatggc tacgtttaag gagggtttgg
     5821 gtcaaacctt gataaaccaa taaagaccct gatatcacga gtaaggctat taacgcctct
     5881 agaagggtaa aagcttttat gttacttaat tgtttactca atcttcatca cttctccttc
     5941 gtcatcgtga atactaatat atctatccgt acgtaaaata gtaatatgac aaccattcaa
     6001 tgtcatctct ttagttctag tttgtactgc catcaaggct agattaagct tttcttgctg
     6061 ctttcgatag tacgttagag cagcctgact ctgctgtaaa ctgcttaaga tgagaatgac
     6121 aatactaaag aggatgcctg ttgcgatcat actttcaagg gctatgtaag ctttgacttg
     6181 ttttttgata attcccactc cctagataaa attggtattt aaagcgtcga tcagtcatgt
     6241 caaaaataat tttagctaag gaatggttgc ctcccatctg atcaataact aaccgataag
     6301 gatgattcac tgttattgat ttaggaactg ttaggctatt accttctgta acaatttttg
     6361 ttgaagaaat atccaagacg cgctgttttt gctgtaatat ggctaacttt tgctgatgtc
     6421 gatacaaatg ttcaaaatgg ctaaaaaaca gatgctcttc tactttttga tagctttttg
     6481 tcactggtac agacaatccc aatataatga aactcatcac tgacaaactt agtaaagttt
     6541 caagcaaagt aaaagcttta attgctaaga cgggcttttt catttttatg tttgtcataa
     6601 tagtcttgat aagctttttc ttgtttctca gtgatactac catctgcctt taactggctc
     6661 aaacttggtt ttgagccttg agataattca tatagttctg cttgattctc cactaattta
     6721 acaacagcgg cattacctgt ttctgtaacc ctgtcttttt gcttgcttaa attaggcaca
     6781 aataatagca tcaaaacact gataacaaga atcaccaata acatttctag aagagtaaat
     6841 ccttttagat tcttgtgtcg taagttgttc cattgattaa tcatcttaaa aaatacctcc
     6901 catattttgg taaattggta ataggattgc cgcataaatc atgacgattg tcacagcaac
     6961 tactaaaaaa atagctggtt gaataagttg tgtcacttga taaagctgac taaaaaattg
     7021 ttcccagctt tcctgggcat agatttccaa ctcagccccc agtttcgatt tgatttcacc
     7081 gtattcaatc attaaactta attctttttt aaaaaaaggg taggtagcta ctttaatgtg
     7141 aaaggcttgc ccttcaagca gactcattct gatgtcctca gctaattctt tcattagtga
     7201 ggatttctca atggccataa tgtctaaaat agtcatcaaa tcaagacctt gacctattaa
     7261 cgtaccccat tctctagcat aataactagt taagtattgt tttaacagtt tacctaaaaa
     7321 aggataacgg cttagccgac tatagagttt caagcggctt tgagatcgcc atcgtagcca
     7381 taccatcccg aacaacaaaa taagacctga gcaaaaacct ataaagaagg caggaaaatg
     7441 gttaagaaag taagttatct gattttgagt ttctaattga gggactaaat aacgtctcaa
     7501 ccctagcatc atcacaaaaa gaaaaagtaa aagaataaga ggataggtga taacttcaac
     7561 agtttttcgt ctgatccttg ccatttggtt caagtaatgt tgaatcgcta ctaatgttgt
     7621 ttcaatatta ccgtgtctat cagctaaact aatttgagta agaatagcat ctgaaaaacc
     7681 taaacccgac aacatatctg ctaagccttg tccttttaat aaagactctt ccattttaag
     7741 aacataatct agctgaagca actggcttct ttttaaaaaa gcaataacct ctgcgaagct
     7801 aaatcctgta gataaaagat tagctagtaa ttgaatgaat ttatgctgat gtttactaga
     7861 taattttttc aagcttagct tgcttgggat tgagatgtcc tgcctcaaga agctgatcaa
     7921 tttgttgatt ccagttcgat gaggaataat attcaaattc gttttgagta gagtcaatca
     7981 atgctcctcc attaagtaac ctttggtaag caattaatgc taagcaatta gacagttctg
     8041 ctttcgttat accaagttct aacaatcttg cataaacacc cgagatactt ttagcgtgaa
     8101 ccgttgaaaa aaccatggca cctgttagac tagccctaat aacggctctt gctgtttgac
     8161 tatctcgaat ctcaccaata actaaaatat ctggtcgatg gcgtaaagac agtttgatca
     8221 aattgtcata agtcatacca atatcatcat tcacttgtaa ttgtaaaatt tggtgatttt
     8281 taatttctac aggatcttct atactaataa cctgtgcttc ttgatgataa ctcgaaatca
     8341 gctggtacat caaggttgtc ttcccagacc caactggtcc tgcaaataaa tacaaccctc
     8401 taccgccaac ttgattggct acttttgtca atccatcaaa ccaataatgt acacttttat
     8461 tttgatgatg aagcagacga atcactaagc tttcttgccc gcgataatca cccacgctag
     8521 ataagcgcaa agataactgc ttatctttac taagcttata atcacatgaa cccacctgac
     8581 aacgtctctt ttcaccaact atcattcctg caacgaattt aaagtgacta ataagaggag
     8641 ccatccgatc gctctgataa acatcaacta atctcctttc atctcctatt cgtaaaaaaa
     8701 gatcatattg atctgctctt ggcaaaatat aaatatcttg tgcctgaacc tgttcagctt
     8761 ttgctagaat agcttttgct aatgcttgta ccataattcc tccttactca tttattcgta
     8821 aacaaaaaaa gaaaatatca taagaagtga tattttcttt taact
//
LOCUS       DAUELZ010000069         8665 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.070, whole genome shotgun sequence.
ACCESSION   DAUELZ010000069 DAUELZ010000000
VERSION     DAUELZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.070"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            146..307
                     /gene="sagA"
                     /locus_tag="VHI09_001243"
     CDS             146..307
                     /gene="sagA"
                     /locus_tag="VHI09_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEQ6121119.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            529..1479
                     /gene="sagB"
                     /locus_tag="VHI09_001244"
     CDS             529..1479
                     /gene="sagB"
                     /locus_tag="VHI09_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEQ6121120.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1476..2534
                     /locus_tag="VHI09_001245"
     CDS             1476..2534
                     /locus_tag="VHI09_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEQ6121121.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENALEPETFANLMEVISGLCYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNDPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQILPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2554..3912
                     /locus_tag="VHI09_001246"
     CDS             2554..3912
                     /locus_tag="VHI09_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEQ6121122.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3887..4558
                     /locus_tag="VHI09_001247"
     CDS             3887..4558
                     /locus_tag="VHI09_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ6121123.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPQITLSFESIDSKTFFMGLVFCVLIVLIELVFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYSSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKEVT"
     gene            4555..5238
                     /locus_tag="VHI09_001248"
     CDS             4555..5238
                     /locus_tag="VHI09_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEQ6121124.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLVALSYQDFLDV
                     LLSLFQFVIIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIAILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILMVT
                     YLMISCKEY"
     gene            5261..6184
                     /locus_tag="VHI09_001249"
     CDS             5261..6184
                     /locus_tag="VHI09_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121125.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6193..7320
                     /locus_tag="VHI09_001250"
     CDS             6193..7320
                     /locus_tag="VHI09_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121126.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIRLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            7317..8435
                     /locus_tag="VHI09_001251"
     CDS             7317..8435
                     /locus_tag="VHI09_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121127.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSN
                     LSRFRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGGTLSQAWPHLLALMGTALALIIFSSLLLKPTRL"
BASE COUNT         2701 a         1331 c         1644 g         2989 t
ORIGIN      
        1 tgataagaac tagatagttg ttgtgttaca acagtacaat tgagctagcc ttgtccttgt
       61 tgtgttaact ttatttttaa aataaggtta aaaataaacg actcgtgttc ttatcagtta
      121 cttattagat aaggaggtaa accttatgtt aaaatttact tcaaatattt tagctactag
      181 tgtagctgaa acaactcaag ttgctcctgg aggctgctgt tgctgctgta ctacttgttg
      241 cttctcaatt gctactggaa gtggtaattc tcaaggtggt agcggaagtt atacgccagg
      301 taaataatct atttagcatc tctatgtgat agtgatatta aggtaatgag tttgctagca
      361 actaagttat ctttggtaac ttctgattat agatgaatgg catagaggtg ttagaaaaca
      421 tgagacaaaa gtaatcttct tgatgctaac gtctcaggta attagcaggt actagacagt
      481 acctgctaat tactatatgt ttagtaaaat gagataggaa agatagttat gtcatttttt
      541 acaaaggaac aacaacctaa agagaattgt ccgccaataa ctgttgaaaa agcaaggcaa
      601 ttgtttgaat ttaatacaaa ccacttgtcc ttatcggatt accatcatca aacggtgcta
      661 aaaacgtcaa agcagctagt tgctcaacat ttaatgccta atgcaaccga taatcttagt
      721 caacattttt tgatgaacta taaagctaat aataattatt taggcttcca agctagtatt
      781 gtcgactttt ttacagattc tgccgttgct aatttttcaa gtagttacgt ttatgaaagt
      841 caggaaaaga taattcgttt accaaaacct accaagatat caactgctct gtcgacatgt
      901 attataaaac gaagaagtca tcgtcaattt tcagatagac aaatgcctct tcaagattta
      961 tcaaacattc tttattatgc atgtggtgtt agttcacaag catcaattag agatggagca
     1021 tcagataaga ttacactcag aaactgtgct tcaggtggag gtttataccc tattcattta
     1081 gttttttatg ctagaaacat cagtaaatta atagatggtt tctatgaata tctaccctat
     1141 cagcatgcac taaggtgtta tcggcatagc tctgaggaaa acgttagaga ttttgcggaa
     1201 tacggtgcta ttaatgctga aaattgtaat attattatta tttatgtcta ccattacatg
     1261 aaaaatacac gtaaatatgg gaatcaggcg actgcctatg cttttattga atcaggagaa
     1321 atagcccaga atattcaatt gactgcaact gccttaactt atggaagtat tgatattggt
     1381 ggttataata aggaatatct ccaagaatta ttagatttag atgggctagg agagcatgtg
     1441 attcacatga cactcgtagg aactaaggag tctcaatgaa atatcaactt aatagtaatg
     1501 ttcgtgtcgt gacatttcaa gatacatttt gttttagaaa aggaatctgg gactttaatg
     1561 aagccattct agatttgaca caagaacctc aaaatttaaa gatagtttat caagaaatag
     1621 tttcacagtt ggttaaggga gtagccattg atactgaaac ttatgaaaat gctttagaac
     1681 ctgagacgtt cgctaattta atggaagtca tttcaggctt gtgttacaat gatatgctga
     1741 tgcttgagga tgactacaat cttgaagaaa atgtcatgaa aattttaatg ggtaatttcc
     1801 gttttatggc gcaagcaggt catatggtaa gcaatgatcc tgttttgttt atcagtgatt
     1861 cgacttatgt taatgaatct gctgagttgc tagctgaaca actgcattta caattgcaag
     1921 tggctagcga tgacttaaaa atgttaattc agcagacaga tgttagttcg cgtttagatg
     1981 ctttggagca tcgccgtaac atgaattgtt tatcagaagc cttgtgcgat taccaaagta
     2041 ttattatttg tcaagaacgc ttgaatatta tgatgttgcg acacttgaat gaagttagcg
     2101 tagctatgaa aaaacaatta gtgattggtt ttgtagatgg accattcttg cacacttgta
     2161 ctttgaatcc tcctcatagt gctgactttg atagcttaga aagaagagtt ttggcacgtt
     2221 tacaagactc aactctctat caacattttg ctaatcagat tttaccagca acacaagatg
     2281 tgagccaggc ctatttacca ttgttaaatg ttttaatgaa tttagtcgtt agcgaagctt
     2341 tcattattgc tcaaacaggt agttctaaat ttgagggacg tttattaagc atttacttac
     2401 cgacgcttga gattcaggtc caagatattt tgaaaatgtc taattcacag acacaaggag
     2461 ctttggcaaa attaaaatat gaggatcagc agatatcaac acgtgagatt gttaaaaaac
     2521 tccttgacga gtaacctata aggagaatct attatgttat actattatcc ttcttttaat
     2581 catatttttg atgaattaaa aagtttaagt ggcaatcgaa caggtatctt aaatcaatct
     2641 caagttccag tttgcaacca tcctcacgat gtttacttaa aaagtattac tggccaaatg
     2701 ccagattatc ataaacaatt tataggtgag ttgagtcaag ttagttatca cattattggt
     2761 tacggcagtt actatgaaga agcacttatc aagtatttag gagaaagtat tgaacgctat
     2821 gctactgtaa ttgccggaga cttactatca gatcggattg tctatgcttc ttataatgag
     2881 ttaaaactgt tacataaagt gatgccttta gaatacttgc aagtgtttac tcaagaacag
     2941 atcgcattat cttgtgactt gcaaatgatg atgtgcgata aaatggtaac tgagaacgat
     3001 gtattgggct gggttaaatg tcccatgttc tttgaggacg cagagatgta tgttccagct
     3061 caaatgcttt gtgttgggta taaaacaaat gagactgtag gagaaagacg gataatacca
     3121 ggattttcta cgggaacagc ttctcacaaa acgttagaag cggctatgtg caacagtttg
     3181 attgaataca tccagatcga ttcaatgatg cttagctggt atactaagaa gccttgcccc
     3241 aagattattg ttgatgatcc tgatattgaa gtgattttag aagaagctag actgggaaaa
     3301 gacagtctat acgacattat tcctatcgat atgactgtag gagaagataa tcctctttat
     3361 acgtttggaa tcattctcaa aaataaatat gatgaagggc cttacctctt gtttggagtg
     3421 caggctgggt tagatcctaa acatgctttg ctaagaggga tcatggaagc ttcagctatc
     3481 agctatagtt actactataa tcttttatat caaaaagctt cccttgcaaa tattgaatgc
     3541 gaagaacctt tgtttttaga tttagatagt aatgttttct attatgcaca tcccaaggac
     3601 caagaccata aatggaaggc ctttgaacct ttgatttcag gagaagttct gttaagtgat
     3661 ttggaagatc attctggtaa agataaaaaa gaagatttga agactttatt agcttatgct
     3721 aagaaagtca gtcctaatgc tgtcttttta gatattacac ctcctgaagc tctagaaaaa
     3781 ggctggtacg tcacgcgagt tctgatgcca gaacttcttg aaatgtgtat accagcattt
     3841 ccttttgcta atcacccacg aatgagacag tttggaggtg taacaaatgc ctttgtccat
     3901 ccaatgcctt aatctttgtt ttttgttggt aactttttgt ccttcaattc ctatgcaagc
     3961 tatttttgga aaagaagata gtggttatgc atttaattta attggttttc taagagcaac
     4021 tctgatttat gacattttgg ctttggtaag tatttatgtt ttgtcacctc aaataacctt
     4081 gtcttttgaa agtattgata gtaagacgtt ctttatggga cttgtatttt gcgtcttgat
     4141 agtactgatt gaactcgttt tcttacatgg tttacgttgt tggcaaaaaa agcaatggct
     4201 tcctgcaact ttctcgtttg taggaacgac aaatgattgg tctaagattg gctatccttt
     4261 attactagct ttatttgaag agacgattta tcgttttttg tggtttaata tattagcttt
     4321 tcaatggcat ttaccaacaa ttattgtttt aattgtaact agtttttgct atgctttaaa
     4381 tcacttattg atggggaaat ccatttttta tgctaagttg gtaacaggca tcatttatag
     4441 tagtatttac atgctgacta gtcaattgtg gttggttgtg ataatgcatg taggagggaa
     4501 cttgctagtt gagtgtctta gccatcttca aacaaaaaag aagaaggagg tgacatgatg
     4561 ctattggttt tgctgtcgtt tttaggtcta actactcttt ggttgaccag ttatcgaaga
     4621 cgttgtttgg ctagatggca tcttcaatgg ttagtggcct tatcctatca agatttttta
     4681 gatgtcttac tgagtctgtt tcaatttgtc attattatac ttgttttatt cttttactca
     4741 gctactatta atctaggaga ggttctgacc tttttgacac aaactagctg gcattggcag
     4801 attctttgtt atttagtgtt gtatcttatg gccatcatag agatgacgct tttagtttta
     4861 attcttatct ttgatgtttt gctccagaaa gatagtcgat tgctctttaa aaaaataact
     4921 tggctgcctt ttagacaaga taaaccagtc ttaagtatac ttttgctagg catagctatt
     4981 ctagtagaca gtctctttta tttgggcttg cttatattat taggggaaca gtcgctaaca
     5041 agtttagcaa cgcttatttt aggttatggg atagtcaagg cttgtcgcta ttcaggctgg
     5101 ttaaatgtac tattagcttt ttgtcttttc actatagttg gactatgggc agttacggca
     5161 actttattat atggttggct agtaggtgcg atgatattaa tggtgactta tttgatgatt
     5221 agttgcaaag agtattagca atattaaaaa ggagtgatat atgagttttg tacaattaac
     5281 aaatgttgtc aagtcctaca aaaacggcaa gaaagctgtc aatgatgttt ccttgtctat
     5341 tgaagcaggt aatatttatg gtttgttagg accaaatggt gctggtaagt ctaccttgat
     5401 taatcttatc ttaggcttga tccctttgag ttccggtaaa attactgttt tagggcaatc
     5461 ccaaaagact attcgaaaaa taagttcgca gataggttat gttcctcaag acattgctgt
     5521 ttatccagac ctaactgctt atgaaaatgt agaactattt gggtcacttt atggcttaaa
     5581 gggagctcag cttaaaaaac aagttctaaa aagtttagaa tttgtgggac tacactccca
     5641 agctaagcag tttccaagtc aattctcagg aggaatgaag agacggttaa atatcgcttg
     5701 tgcgctagtt cattcaccta aattaatcat ttttgacgaa ccgactgtag ggattgatcc
     5761 tcaatcacgt aatcatattt tagagtcgat tcgtttgcta aacaaagaag gtgccacagt
     5821 tatttatacg acccattata tggaagaagt agaggctctt tgtgattata tttttattat
     5881 ggatcatggt caagttattg aagaaggacc taaatttgaa ctggaaaaac gttacgttgc
     5941 aaatctagca aaccagatta ttgtaactct aacagattca cgtcatttgg aactggcaga
     6001 taagcctgat tggtctttga tagaagatgg agaaaaactc atgttgaaga ttgataatag
     6061 tgatatgaca tcagttgttc atcagctcac acaggccaat attactttta gcgagattag
     6121 acataaccat ttgaatttag aagaaatttt cttacactta acaggtaaga agttacgaga
     6181 ttagaggtta ctatggtttt atttcattta atcaaaaaag aaagtttaca gatttttaga
     6241 aaccgaacag ccttattgat gatggtgatt tttccaattt tgatgatcgt tattttaagt
     6301 tttgccttta aatcaagttt taatactgcg acaacagtcc ctaaattgac cattcgttac
     6361 caattagagg gtgaaaaaac ggattaccag aaaaattttc ttgctttttt aaaagtttta
     6421 aaccaaaaac ttcatttaga ggctaaacct agtaattctc ttgaaaaaga tcgacaaagg
     6481 gtcagtgaag gagccttaac ggctgtttta gaagtgaaga agaatcagac cattaaggtt
     6541 attactaata atattaatca gcaaaatgca gatttaatca atatgctagt aaaaaattat
     6601 gtagataatg ctaaaactta tgactcgata gcagctcttt atcctcaaca attaaatcat
     6661 atcagaaagc gaagtgtgga ctatgttaag gtcagttcaa tacagacaag taaaggaatg
     6721 acatcagctg attattatgc tatttccatg tttaccatga ttacttttta tagtatgatg
     6781 tctgcgatga accttgtttt gtcagatcgt caacaacgga ttacaaatcg tattcgctta
     6841 acaggagttt ctccaagtct tttagtcttt gggaaattaa taggtgctat gttagcaaca
     6901 actgttcaat tgagtctttt atacattttt acaaggtttg ttttacgagt taattggggc
     6961 actaatgagt ggatgcttat tggtataaca gcctccttag tctatctctc tgtagctata
     7021 ggtatcgggc taggcataag cattaaaaat gaggcttttt taacggttgc atccaatact
     7081 attattccta tattcgcctt tttaggaggc agttacgttc cattgacaac attacacagc
     7141 tctattatta atcaattgtc gaatatatct cctattaaat gggttaatga tagtttgttc
     7201 tatcttattt ttggtggtca atataatccg attcctgtaa ctttgatcgt taatattagt
     7261 atcggaacaa tttttataat attggcatta ataggtatga gaaagcaggt gacgacatga
     7321 tttgttttat aaaaactcta tttgttaaga taaagcgcaa aaaaacaagt tatgtgacat
     7381 tctttttaat gcctatttta acgacacttt tggctttatc tttgagtttt tcaaataata
     7441 atcaggctaa aattgggata ttagataaag ataatagtca gatatctaaa caatttattg
     7501 ctcaactaaa gcaaaacaaa aagtacgaca tttttaccaa aatcaaaaaa gagcatattg
     7561 atcactacct tcaagataaa tctctagaag cggtattgac tattgataaa gggttctcag
     7621 ataaagtgtt gcaaggaaaa tctcaaaaac tcaatatacg ttcgattgct aatagcgaaa
     7681 ttacagagtg ggtaaaggca cagaccaatt atctattgga aaactataat attatagggg
     7741 atgtggctct aggtaacgaa gatactttta atagaattct ccaaaaaaac caacagttaa
     7801 actatgatgt taagcaagta actttgacag accgatcacg tagcaaagca gtatcttcca
     7861 caacgacagg atttttgctt attctaatgt taggtagtac gagcgtgatt tacagtggta
     7921 ttttggcaga taaatctagt cagttatacc atcgattaat gttatcgaac ttatctcgtt
     7981 ttcgctatat gttgagttac gtctgtgtag gatttgttgc ttttacgatt caaattgtca
     8041 ttatgctgag tttgttaaaa gtatttaata ttagtttttt tgttccgaca tcacttcttt
     8101 tgattatttt tttccttttt agtttattag cgataggctt tggcctgctg attggagcta
     8161 tcacacaaaa ttcccaacaa agtagccagt tagctaatct tattgtaatg ccgacttcaa
     8221 tgttggctgg ctgtttatgg ccattgtcta ttacgccttc ttatatgcaa gcaattggta
     8281 agttattacc acaaaattgg gttttatcag ccatagctat ttttcaaagt ggagggacct
     8341 tatctcaggc ttggccacac ttgttagctc taatgggaac agcactagct ttgattattt
     8401 tttcaagttt attattaaaa cctaccagac tataattagt cttttgttaa aaaatacttt
     8461 cctaattagc taactaccag gtagcccttt tattggctta tatgcaataa aagggttgct
     8521 ttttgcttat attttctcta gtgtacttat cttttgttaa aagtgattgc gatgttactg
     8581 ttgttactgt gttttttaga gagcatatta tcaaaagcga cctctctaga aagttcgttt
     8641 ttgttgataa atgagttgtc aaagt
//
LOCUS       DAUELZ010000070         8590 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.071, whole genome shotgun sequence.
ACCESSION   DAUELZ010000070 DAUELZ010000000
VERSION     DAUELZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.071"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            154..1446
                     /locus_tag="VHI09_001252"
     CDS             154..1446
                     /locus_tag="VHI09_001252"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEQ6121128.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHNEGVTTDNLRISDRA
                     QVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLDFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPANLEQLKRCKPIYEELPGWQEDITGVRSLEELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            1773..2816
                     /locus_tag="VHI09_001253"
     CDS             1773..2816
                     /locus_tag="VHI09_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ6121129.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSAKQTTDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGNALVKVAVKTSEDQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIEAAK
                     KAIIEGTITVPEN"
     gene            2989..3528
                     /gene="nusG"
                     /locus_tag="VHI09_001254"
     CDS             2989..3528
                     /gene="nusG"
                     /locus_tag="VHI09_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEQ6121130.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            3899..5254
                     /gene="nga"
                     /locus_tag="VHI09_001255"
     CDS             3899..5254
                     /gene="nga"
                     /locus_tag="VHI09_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEQ6121131.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFASPM
                     HIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYQYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     KYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            5259..5744
                     /locus_tag="VHI09_001256"
     CDS             5259..5744
                     /locus_tag="VHI09_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEQ6121132.1"
                     /translation="MYKVPKGLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILEYGVYPAFIKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANMTKVLKDAHIREAMAHYKLGEFAHYQDTMLDMVERTIKT
                     F"
     gene            5768..7483
                     /gene="slo"
                     /locus_tag="VHI09_001257"
     CDS             5768..7483
                     /gene="slo"
                     /locus_tag="VHI09_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEQ6121133.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKPESSELTTEKAGQKTDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFAPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLINQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFAKSVTFKELQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRT
                     EYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            7738..8091
                     /locus_tag="VHI09_001258"
     CDS             7738..8091
                     /locus_tag="VHI09_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121134.1"
                     /translation="MKQQSYQPLRFVYLLVALFAALLLIARPVMADEENDPWILGYKQ
                     GLTKGKEAGKAADWTDLTPPDQEPPSDIEEANKEGYKDGYRDGYNEGFGESYPLLIPV
                     KFIWGIILHWWGKLI"
     gene            complement(8375..>8590)
                     /locus_tag="VHI09_001259"
     CDS             complement(8375..>8590)
                     /locus_tag="VHI09_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121135.1"
                     /translation="SLFMIATAAVVGLSLATPENQTVSANAQTPAVQNCENGCCDRPF
                     FGYCGPFSWLYYNTRYFLGYSDTATNQ"
BASE COUNT         2888 a         1491 c         1812 g         2399 t
ORIGIN      
        1 gctagcaggt gagattcctg cctgttttcc ttaaatatca agagctacat tgctgatctt
       61 acagatctga ccttcttttt ggtttttaag cagtggttag cttgcctatg atttttattg
      121 agaataagtg tttcagaaag ttaggacaat gatatgacat ctgtagttgt tgttggtacc
      181 cagtggggtg acgaaggtaa aggtaaaatc actgatttct tgtcagcgga tgcagaagta
      241 attgctcgct atcaaggtgg tgataatgct ggtcatacga tcgtgattga tggtaagaag
      301 tttaaacttc acttgatccc atcaggaatt ttcttcccgc aaaaaatttc cgttattggt
      361 aatggtgttg tggtcaaccc caaatctttg gtcaaagagt tggcgtacct gcataatgaa
      421 ggagtgacga cagataacct tcgcatttca gaccgagctc aggtcatttt accttatcat
      481 atccagctgg atcaactgca agaagatgca aaaggtgaca ataaaatcgg aacaactatt
      541 aaagggattg gtccagctta tatggataaa gctgctcgag ttggtatccg tatcgcagat
      601 ttactggata aggatatctt tgcagagcgc ctacgcatta atttagctga gaaaaaccgt
      661 ttatttgaaa aaatgtatga cagtacacct cttgattttg atgctatttt tgaagaatac
      721 tatgcttacg gtcaagaaat caagcaatat gtgacagata cttctgtaat tttaaatgat
      781 gcacttgatg ccggtaaacg tgttcttttt gaaggtgcac aaggggttat gcttgatatt
      841 gaccaaggaa cgtacccatt tgtaacgtct tcaaacccag ttgctggtgg tgtaaccatt
      901 ggttctggtg ttggcccaag taaaatcaac aaagtagttg gtgtctgtaa agcctacaca
      961 agccgtgtcg gtgatgggcc attccctaca gaactctttg atgaagtggg tgagcgcatt
     1021 cgtgaagtgg gtcatgagta cgggacaacg accggccgtc cacgtcgtgt cggttggttt
     1081 gattcggttg tcatgcgcca cagtcgtcgt gtatcaggta ttactaacct ctctctgaat
     1141 tcaattgatg ttctttcagg gcttgatacg gttaagattt gtgtggctta tgaccttgat
     1201 gggaaacgta ttgactatta cccagcaaac cttgaacaac tcaaacgttg caaaccaatc
     1261 tatgaagaat taccaggctg gcaagaggac atcacaggtg ttcgtagcct tgaagagctt
     1321 cctgaaaatg cccgcaacta cgttcgtcgt gttggagaat tggttggcgt tcgcatttca
     1381 accttctcag ttggtccagg gcgtgaacag accaatattc ttgagtcagt ctgggcatct
     1441 atctaaggtg tctagtatga caaagagttg gatagcgttt ccagctcttt ttaccgtttg
     1501 ttggattgta tagttagtca tgctattact agttgcttga gttttgtcgt tgtcttatct
     1561 ccttaagagg tgatttttaa ggagataatg ggttttacca gttgatctca ctttctcgtt
     1621 cggtcatttt ggtgaaaaag agatttttca gcaaaaatag taggataaaa ctgatttttt
     1681 tgatatacta aacttttcgt tctagagcat aagtagaacg ttaacaatga cacatcactt
     1741 taggattaaa catttaggag gttcattttc acatgaacaa aaaagtaatg tcacttggtc
     1801 ttgtttcgac tgccctattc acattaggag gctgtaccaa taactccgct aaacaaacaa
     1861 ctgacaattc attaaaaatc gctatgatta ctaatcagac gggtattgat gacaagtcat
     1921 ttaaccagtc agcctgggaa ggcttacaag cttggggaaa agaaaataaa cttgaaaaag
     1981 gaaaaggcta tgattatttc caatcagcca atgaatcaga gtttaccaca aaccttgagt
     2041 cagcagtaac caatggttat aatcttgttt ttgggattgg atttccatta catgacgctg
     2101 tagaaaaagt agccgcaaac aatcctgaca accattttgc aattgtggat gatgtgatta
     2161 aaggtcaaaa aaatgttgca agtatcacct tttcagacca tgaagcggca tacctagccg
     2221 gtgttgcagc agctaaaacg acaaaaacca agcaagttgg ttttgtaggt ggtatggaag
     2281 gagatgttgt caagcgcttt gaaaaaggtt ttgaagctgg tgtgaaatca gtagatgata
     2341 ccatcaaagt aagagttgct tatgcaggct cttttgcaga tgctgccaaa ggcaagacga
     2401 ttgcagctgc tcaatacgct gaaggcgcag atgttattta tcatgcagca ggaggcacag
     2461 gggcgggtgt ctttagcgaa gctaagtcta tcaacgaaaa acgtaaagaa gaagataagg
     2521 tttgggttat tggtgttgac cgtgaccaaa gtgaagatgg aaaatacact acaaaagatg
     2581 gcaagtcagc taattttgtt ttgacctcaa gtatcaagga agtcggaaac gctttagtaa
     2641 aagtagccgt aaaaacctca gaagaccaat tcccaggtgg tcaaataacc acttttggtt
     2701 taaaagaagg tggtgttagc cttacaacgg atgctctgac acaagacact aaaaaagcta
     2761 ttgaggctgc taaaaaagcg attatcgaag gaaccatcac agttcctgaa aactaaaact
     2821 gtacaatacc gtccagagtc gtcacgaacc gactttggac tttttaataa aggtgtcaag
     2881 tagctccaca gatacgttga cagcagccct gccttttatt ataatgaagg tgatagaaaa
     2941 cagcgcaaaa gccaaaaaaa gggctttttt ataatgaaag gaaacatcat gctagattca
     3001 tttgataagg gctggttcgt cctccaaact tactcaggct acgaaaacaa agtaaaagaa
     3061 aacctcttgc aacgtgccca aacctataac atgttggaca atattttgcg cgtcgaaatt
     3121 ccgacacaaa cggttaacgt tgaaaaaaat gggcaaacca aagaaatcga agaaaatcgc
     3181 tttccagggt atgtcttggt agaaatggtc atgacagatg aagcttggtt tgttgtgcgt
     3241 aacacaccaa acgttacagg ttttgtggga tcacacggga accgatcaaa accaacacca
     3301 cttcttgaag aagaaatccg tgctatcttg ttgtctatgg gtcaaaccat tgatgtcttt
     3361 gataccaata tcaaagaagg cgatgtggtt cagatcatcg acggtgcctt tatgggacaa
     3421 gaaggtcgtg tggtagaaat cgaaaacaac aaagtgaagc tgatgctcaa catgtttggc
     3481 tctgaaacag tagcagaagt tgaactttat caaattgcag aactataggc tctcaaaggt
     3541 ctgatggtat aaatattacc atcagacctt ttttagttgg cagtgaagcg tgtttacaaa
     3601 caaatggatg actattgtta acatcattgt aatcagagat aattaacaga tctattactg
     3661 ataacggtgc tactatcaaa tatttaacta gtacttttaa tctggtttaa atagaacaga
     3721 tgtgaaggtt ctgtgataat aagatggaaa tatccccttg aaacttgtaa cagttggcta
     3781 cttgtttttt tgtgttcatt tttttgatag aaaagcctaa aacgatgtta ataaagcgca
     3841 ttgacaaaaa ctagttgtta atgtatagtt tacttaaaaa taatataagg tggtttacat
     3901 gagaaacaaa aaagtaacat tagctcatat tgtcgcaaag acaagtgttg ctattgcttt
     3961 ggctggagca atgggtagca gtttattagc taatagcaca acgtacgctg ttagtggcaa
     4021 agaaaataaa aaaagcgatg tcaaatatga aacgaccaaa gttatggaag ctaacgcaac
     4081 ttcctctaaa gaagacaatc atgtcatgca cacattagac ggctcaatga gtactgtttg
     4141 ggaggaaaat tcacctggtg gtggtgttgg tgaggtactt tcctacaagt ttgcgtcccc
     4201 gatgcatatt gggagaattt taattgttaa tggagacaca tctagcaagg agaattacta
     4261 caagaaaaat agaattgcaa aggctgatgt taaatactat aacgggaata aattggtcct
     4321 ctttcaaaaa attgaattag gcgacaccta cactaaaaaa ccgcatcaca ttgagattga
     4381 taaaaaatta gatgttgatc gtattgatat tgaggtaaca gaggtccatc agggacaaaa
     4441 caaggatatt ttagccttgt cagaggtcac ttttggcaat atggaacgcg atctttttga
     4501 aaaaaagttt aaagaaatta aagataaatg ggtgacagat aaacaagcag atgagtttat
     4561 tgaaactgcc gataagtatg ctgataaagc tgttcagatg tctgctgttg cgtcacgtgc
     4621 tgagtattac cggatgtatg ttagccgcaa ataccagtac aaaaaagagt ttgttgaaaa
     4681 actaaaacaa gtctacaaag aaagcggagc ttctcacgtt acaagtaaaa aagatttgat
     4741 gttagctttt gatgatgcta aaagaaagtc aacgattggt cgacaagaaa acggtctttt
     4801 tgtgacaagt tttgctgaag atatggcatt gctctttact gatcaaggta agttaaaatc
     4861 agctgaccaa attgaaaata taaaaggtgt cgatagcgga aaatatagcg atggggttta
     4921 tcaatatgag tacgattctg aactaacaaa aaacattgat aagctaggct atatccgaac
     4981 agctagcgga gatactcctg gagcaaattc gctcaacatt cctggttgcc aaacgtggtc
     5041 aggaaaacac attgaaaatt cagaaagtga gttaattttc ccatcgatta gtgttaaaga
     5101 tctaaaatct aaagctgtct tagcagagat tgatgccaaa ggctattttg aaattattga
     5161 tcctaccatc attgctccaa atggtgacca taaaaaagta actggtcgct tcaaaattaa
     5221 gaaaatgcaa gatagaatgc aagataggaa gtaacaatat gtataaggtg ccaaagggtt
     5281 tagaacatta ccaaaaaatg tttcaaaaag aagtaactgt taatgattta aaaaaatact
     5341 tgataggtag tgacaaagag tatcgtatca ctagaagaga ctcctatatg ggagatattt
     5401 ctgatccaga agttatttta gagtatgggg tttaccctgc tttcataaaa ggctataccc
     5461 aattgaaagc taacatcgaa gaagcattat tagaaatgtc aaatagcggt caagcattag
     5521 acatttacca agcggttcaa accctaaacg ctgaaaacat gttattaaat tattacgaaa
     5581 gcttgccatt ttatttaaac cgtcaaagca tactagctaa tatgaccaaa gtgttaaaag
     5641 atgcgcatat tagagaggct atggcacatt acaaattagg agaatttgct cactatcaag
     5701 atactatgct tgatatggtc gaaagaacaa taaaaacatt ttagaatgat aaaaaggtat
     5761 gaaggacatg tctaataaaa aaacatttaa aaaatacagt cgcgtcgctg ggctactgac
     5821 ggcagctctt atcattggta atcttgttac tgctaatgct gaatcgaaca aacaaaacac
     5881 tgctagtaca gaaaccacaa cgacaaatga gcaaccaaag ccagaaagta gtgagctaac
     5941 tactgaaaaa gcaggtcaga aaacggatga tatgcttaac tctaacgata tgattaagct
     6001 tgctcccaaa gaaatgccac tagaatctgc agaaaaagaa gaaaaaaagt cagaagacaa
     6061 aaaaaagagc gaagaagatc acactgaaga aatcaatgac aagatttatt cactaaatta
     6121 taatgagctt gaagtacttg ctaaaaatgg tgaaaccatt gaaaattttg ctcctaaaga
     6181 aggcgttaag aaagctgata aatttattgt cattgaaaga aagaaaaaaa atatcaacac
     6241 tacaccagtc gatatttcca tcattgactc tgtcactgat aggacctatc cagcagccct
     6301 tcagctggct aataaaggtt ttaccgaaaa caaaccagac gcggtagtca ccaagcgaaa
     6361 cccacaaaaa atccatattg atttaccagg tatgggagac aaagcaacgg ttgaggtcaa
     6421 tgaccctacc tatgccaatg tttcaacagc tattgataat cttattaacc aatggcatga
     6481 taattattct ggtggtaata cgcttcctgc cagaacacaa tatactgaat caatggtata
     6541 ttctaagtca cagattgaag cagctctaaa tgttaatagt aaaatcttag atggtacttt
     6601 aggcattgat ttcaagtcga tttcaaaagg tgaaaagaag gtgatgattg cagcatacaa
     6661 gcaaattttt tacactgtat cagcaaacct tcctaataat cctgcggatg tgtttgctaa
     6721 atcagtgacc tttaaagagt tgcaacgaaa aggtgtcagc aatgaagctc cgccactctt
     6781 tgtgagtaat gtagcctatg gtcgaactgt ttttgtcaaa ctagaaacaa gttctaaaag
     6841 taatgatgtt gaagcggcct ttagtgcagc tctaaaagga acagatgtta aaactaatgg
     6901 aaaatattct gatatcttag aaaatagctc atttacagct gtcgttttag gaggagatgc
     6961 tgcagagcac aataaggtag tcacaaaaga ctttgatgtt attagaaacg ttatcaaaga
     7021 caatgctacc ttcagtagaa aaaacccagc ttatcctatt tcatacacca gtgttttcct
     7081 taaaaataat aaaattgcgg gtgtcaataa cagaactgaa tacgttgaaa caacatctac
     7141 cgagtacact agtggaaaaa ttaacctgtc tcatcaaggc gcgtatgttg ctcaatatga
     7201 aatcctttgg gatgaaatca attatgatga caaaggaaaa gaagtgatta caaaacgacg
     7261 ttgggacaac aactggtata gtaagacatc accatttagc acagttatcc cactaggagc
     7321 taattcacga aatatacgta tcatggctag agagtgcacc ggcttagctt gggaatggtg
     7381 gcgaaaagtg atcgacgaaa gagatgtgaa actgtctaaa gaaatcaatg tcaacatctc
     7441 aggatcaacc ctgagcccat atggttcgat tacttataaa taggactggt tcaagaggtt
     7501 cgtcaagcac cttgatgctg cttatctctt gagatcttgt gcctactcct ctcacaaata
     7561 cagaaaaaag cttgtctatg agataccttc atagacaagc ttttttgata tactaatcaa
     7621 aaagttattc ttttctcaca tagtgacctt gaagctaagc ttcttctaat ctataatact
     7681 aataagctgc tttttaacaa gcacagcata acataaaagg gagagataac tattatcatg
     7741 aaacaacaat cttaccagcc tctacgcttc gtctacctct tggtggctct atttgctgct
     7801 ctgttgctta tagcacgacc tgttatggca gatgaggaga atgatccttg gatattaggc
     7861 tataagcagg gtttgacaaa agggaaagaa gctggcaagg cagccgattg gactgatttg
     7921 accccgcctg accaagaacc acctagtgat attgaagagg ctaataaaga agggtataaa
     7981 gatggataca gagatggtta taatgaagga tttggagaaa gctacccttt gttaattcct
     8041 gtcaagttta tatggggaat cattttacat tggtggggga agttgatttg acatttgaca
     8101 atcaactcta agctgactcc atagccagtg ggtatagtta gacgcagacg aatttaaaac
     8161 agttccttgc taccacacct taaactgcac cttaatcatt aggctttagt gatatgtgtc
     8221 caggattctg gagacatagc ctatgtctaa ttgttggggt gcggtttaat tgtttttgga
     8281 tttgaagctg tggcaacacc ctgactttag gacagcaaaa aagcccagga ctgcgtaaac
     8341 aggcttgggc ttttgtctca ctatagaaac aaatttattg gttagtagct gtatcagaat
     8401 accccaaaaa atatcttgta ttatagtaaa gccagctgaa aggtccacaa tacccaaaga
     8461 aagggcggtc acaacagccg ttttcacagt tttgaacagc tggtgtctgt gcatttgctg
     8521 aaacagtttg gttttctggt gttgcaaggc taaggcctac gacagcagca gttgcgatca
     8581 taaataaaga
//
LOCUS       DAUELZ010000071         8462 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.072, whole genome shotgun sequence.
ACCESSION   DAUELZ010000071 DAUELZ010000000
VERSION     DAUELZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.072"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..2170)
                     /gene="shr"
                     /locus_tag="VHI09_001260"
     CDS             complement(<1..2170)
                     /gene="shr"
                     /locus_tag="VHI09_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEQ6121136.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEPLVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKPVPQN
                     KGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDITITVTKKDGQI
                     HFVSAKQKQHVTAEDRQLTKLDVTTLEKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHI"
     gene            complement(2662..4191)
                     /locus_tag="VHI09_001261"
     CDS             complement(2662..4191)
                     /locus_tag="VHI09_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ6121137.1"
                     /translation="MNKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSDNKA
                     EAENQVDDKSTDHSKPTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSRQLPEVRTPI
                     QSLSPYVSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKS
                     TGIAYDSTRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAF
                     DLDPTQASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFT
                     DTTGSGKRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLG
                     YQASSYAWGQCTWYVYNRAKELGYQFDPFMGNGGDWKYKVGYALSKTPKVGYAISFAP
                     GQAGADGTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSK
                     N"
     gene            complement(4278..5378)
                     /locus_tag="VHI09_001262"
     CDS             complement(4278..5378)
                     /locus_tag="VHI09_001262"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEQ6121138.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELKLAITRQVT
                     VTVASLEWLAMAKQEWPDLKGLKVHIKIDSGMGRIGLRSVTEVDNLIAGLKSMGAEVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLPKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            complement(5375..5731)
                     /locus_tag="VHI09_001263"
     CDS             complement(5375..5731)
                     /locus_tag="VHI09_001263"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEQ6121139.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWSGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            complement(5847..8366)
                     /gene="secA"
                     /locus_tag="VHI09_001264"
     CDS             complement(5847..8366)
                     /gene="secA"
                     /locus_tag="VHI09_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEQ6121140.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMASLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAVDAIVTFARTSLVPEESISAKELRGLKDE
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
BASE COUNT         2414 a         1786 c         1485 g         2777 t
ORIGIN      
        1 taatatgatt tctacttgcc acaatagtgc ttaaggcgcg caatttttct attgtttttg
       61 gcagtagtgc taaattattt tgagataagt cgataaaatt aagtgatgtt agaggctcta
      121 gcgctttttc aggtatctcc tcaagactat tttctgcgaa acctaatgaa gtcagacgag
      181 acaatccttc aaatgggtta tcacaaagtc ggccaatacg attcttggaa agatcaagtt
      241 gctccaggtg atgaagcgac tggaatagtc ctccttcaag agagtgaata aagttacttg
      301 ataagtagag ttctcttaat tgagcattgc ttttaaatgt atctttgtct agataagtta
      361 aatgattact tcttaagtta agaaatcgaa gttgcttcat ttgactaaat aactcttttg
      421 gaatttccgt aatttggttt ccatccagat gcagttctgt aatattttta gcatgtttca
      481 ataaagaaat atctgttaac tcgtaatggt ctagacgcaa ctcacctcga atctgttcta
      541 attccgtttt tgataattta ccatcgccgt ctaaatcttt gccaagttta actaaaactt
      601 tttcaagacg ttcagtccca accccttttt ctttatcatc agtttctacc tgataaccac
      661 tccaaccttt ggtaacggtc ttactaaagg tcatgtcaag tttggtgtat ttgtcatagt
      721 ggtttaaatc actttcttga cctcccatgg ctgataccaa aacagcacct ttgtgtaatt
      781 tatccaaatc atcaataggc atggtaaatt cgcttcgaat atagctacca ttgatatctt
      841 tttggccaac ttgtttacgc actgctgctg ggtaggtccc tttgctttca atagaaaagt
      901 caatcaaaaa ttgtcccaaa gcagtattaa gcatggaaat ttccattgta ccatctgctt
      961 taaccactaa tttggctctt ttatcaaaag caccttgaag catggaggac tcttctgagt
     1021 tttctttatt agctttaaag tttagcgtat aggttccttc tgtgagattg ttaatagaaa
     1081 cctcacctgc ttgataagac tcgatggtat cttttaacag tcgatgagta tcggcgagta
     1141 gcttactatc tttgatttct ttgtaagaat ccttgatgac ttgaagtttc tcagccagat
     1201 ctttgaccgc gtctttgttg ctttctttag caataatcgc atccgcttct ttgatagctt
     1261 tttccaaagt ggtgacatcc aactttgtta attgtctgtc ttcagcagtc acatgttgtt
     1321 tttgtttggc agatacaaaa tggatttgac catctttctt ggtaacggta atggtaatgt
     1381 cttcaaatcc ttttgaggat atcacgattg tattttctcc atctttgaaa gcttttgttc
     1441 caagatgaag ccctgtcata ttcgagaccc agccattagt atcttttaca gaatctgaaa
     1501 taagatcttt ataaactgta ccattgactg taacctttgt gatttgctga gtcaatgctt
     1561 ttatctgttt gttatcagaa taacgatcaa tttctggaaa taaaattgca ccatcttctt
     1621 ggctcaattt tgttattaat gacaagttag cagtattttt cccagattta acaacttttt
     1681 gtgtgccttt gttttgtggc actggtttat cactgagcac atcttttttt aatgaatcct
     1741 gatgatctgt tgttcctaat ttttgagctg aaataaaggt cactttatcg ccctttttaa
     1801 taaaggtaat taggatatca ccatcttttt tattaacaat agtcaattca tgcttaccgt
     1861 ctttaataag gttagcattg atcttaatcc cttggtagtt aatatcaaag gttcgctcgc
     1921 cttccaggct tttttcatag acatcattat caacaataac cttttcaata tgtttataat
     1981 actcttttcc agctgattga ctagcgtaag ggcctatctc ttcaactaaa agtcgattat
     2041 cctgagttaa agctactgtt gtcacgagtt gtgactgaac taaaggctct tgtgatttta
     2101 cagtttgagt agcctgaatg agaacaaggg cagatattgc cacaaacgca catttgctga
     2161 tttttttcat tttcaatcaa ctttctaaaa ttttttatga ataagctaac ttttttatta
     2221 actgaactta atctatcact ttgtgattat tttgtcaaga ttgtttccaa tggtccaatt
     2281 gtctgttaaa aagctccact atgtgagcca agacactagc aataagcctg gattgataat
     2341 gatgatatct ctgataaaag aattcctcta caacactatt actgagataa agctagcctt
     2401 aggatagtta atactagggc tgattcaatc attacttcat tcacaaaaca tgtaacacgt
     2461 tataagctcc ataattatgt taggagacaa catgagatgt cactactgca tattcatcac
     2521 aataaaaaaa aagacaatag tcctaatcgt gctattgtct caaaatcatt tatttacttg
     2581 aaactttatc gtgttacacc aacagtttaa aaggcctaac tcccgctaag aaaattaagc
     2641 cttaagaaaa gttattagtt attagttttt actcttgcca ataacatatg ttagctgttc
     2701 agcctgctga gctgtaaagg tacgataaga aatcttacct aagccgatac agttagactc
     2761 tgaaataaga atagacccat cttttctaac atcttctaca attgatacgt ggccataagt
     2821 gccatcagcg cctgcttgcc cgggtgcaaa tgaaatagca taacctactt ttggagtctt
     2881 tgacagggca taccctactt tatacttcca atctccacca tttcccataa aaggatcaaa
     2941 ttggtaaccc aattctttgg cgcgattata cacataccaa gtgcattgtc cccaagcata
     3001 actcgaagct tgataaccca agacatcata actcttagag agcttataac ctgctggcac
     3061 agatgcaaaa ctagctgatg actgcacttt caattcggct ggaatggctg gtgtgccacc
     3121 atggtcatca atccacttat ccaggtcttc cataatttgt gcgcgacgtt taccactacc
     3181 agtagtatca gtaaaataaa cacccccgtc acttgcaaag tttaattgac ctcgtgaaaa
     3241 cttagccgct tttaaatctt gaagtgcaaa attgctattt ttgtttttaa taatggtatc
     3301 ttgtgtcatt ttgacaatag cactatcatc attaaactta cttgcttgag tcggatctag
     3361 atcaaaagct gcatagccaa acatattagc tccaagcaaa gtcgcaatcc cttgagttcc
     3421 taaagaactc tcagacatcg caatagctac gatagctcga acatccagcc cacttttctt
     3481 ttcccattgc aatagctttt caccattaat acgtgtgctg tcataggcaa tgcctgttga
     3541 ttttaagtaa ctatcaatct ggtcagcttt aataccataa cggcgagata aaagatggtg
     3601 ggtataggca tttttaccac tccaatgctc tacatatgcc gcgtatgtgt tgacagaagg
     3661 gatatctatc tcactcaaat ctaaatctga tacgtaaggt gacaacgact gaataggagt
     3721 tcttacctct ggcaactgtc tcgacaatga agaagcaggt gccgtatcaa ctttagatgg
     3781 tgatggtttg ggctgatcag tcggttttgg ctggtcagtt ggtttcgaat ggtcagttgg
     3841 tttcgaatgg tcagttgatt tatcatctac ctggttttct gcttctgcct tgttgtccga
     3901 agaacctaca gcagactgac tagaatcgct tcttttttta tcatccgcgt taacaagact
     3961 attagagttt gtttctgcag aagcatcaag gggaccttgg tcagttttat ctgatgttgt
     4021 aggtgctgca tttttattat tttcctcttt tgaagaagaa tcactggttt catttgtagt
     4081 tgctttatgc gtctcaagca ttacatcttg agccaacact gtcatgcttt ctgctgtcgg
     4141 ggctaagaga cttagtagca tggcaactct taatagtttg tttttattca tatattaatt
     4201 agtcacttct ttctaatgtc ctcactttat tataacatta aagataagca caatgcaatt
     4261 atttttacgt gattttctta atatatccga ggaatacggt cacttaaaag gcatagaact
     4321 tcataattga ttgtattacg gtaatttgcg atatctgttg tagaaatatt tttttgctga
     4381 ttgctgccaa tcaaagtgac ttttgttcct aaaggatatg ctttgggaag tcgtatggtc
     4441 agttgatcca tcgatacacg ccctataatt tcacagaatt gtccatcaac taacaccgaa
     4501 aagccttgca tgttcctggt ccagccatct gcataaccga ttgggacggt ccctacatat
     4561 tcagactttt tggcagtata agtagctcca taaccgactg tatcaccagc tgaaatcatc
     4621 ttgacatgca ctaagctaga ttctagagat aaagcctctt gcagtggaaa tggtaagctt
     4681 agatcagaac cacttggatt caaaccataa ctgacaattc ctaaacgaac agcattaaaa
     4741 atggtatcac tatgccagat acttgtggct gaattactag catgtactaa acgaggctta
     4801 tcctcaagtc cagctatcag ctttttaaaa aactgtaact gctggttaaa tttagtatca
     4861 tctgcctcat cagctgtggc aaaatgagtg aaaatccctt caacttctgc ccccatgctc
     4921 ttcaagccag caatcagatt atcaacttct gttactgagc gaagcccgat tcgtcccatg
     4981 cctgaatcaa ttttgatatg gaccttaagg cctttcaaat ctggccattc ttgcttggcc
     5041 atcgccagcc actctaaact agccactgtc actgtaacct ggcgagtaat cgccaacttt
     5101 agctcatttg gcagcaaaac cccaagaatt aaaatctctt tatcaatacc tgcttgacgt
     5161 aattgcaaag cctcatcaag atttgacaca cagtacccat ccacttgagg taggagtgct
     5221 tttgacacct ggacagcacc atgaccataa gcatcagcct tgacaactgc atacgttttt
     5281 acccctagtg gaatatgctt ttgaacactg gcaacattct ccttaatagc ttgtaagttg
     5341 acccttgcaa ctgttgggcg atggaaactt gaaatcattt tttatcctcc aaaataacac
     5401 tagcctgtac ataattgcca ctatgtgaga tgctaataaa actatttcct ttaaacggag
     5461 attttgtcag aatgggacat cctctaacat catttaaaat ctcaatatct tgaaaggtta
     5521 accgcccaat acctgtccct atagccttag caaaagcttc cttacctgac cagcgtcctg
     5581 ccaaatagct gagtcgacgt ttgtaaggaa aactctcaaa aatagccaat tcctgctcag
     5641 ttaaaatttt ttgagcaaag cgaggatttc tttgataaac tttttcaata gctgaaatct
     5701 cttgtaagtc tattccatgt ccaacaatca ttactttcca ccaaacctta tttcatctta
     5761 atgttagaag atttaaacag tgagaagctg agaattattc tcagcttctt tctatatacc
     5821 taactttgtc ctgttgtttc tcgttcttat gagaaggatt tacgaccgtg acaatttttg
     5881 aattttttgc ctgaaccaca tggacaagct tcgttacgct ccactttggg taaatcatcg
     5941 gtgttagctg actgctgcga ttggatgttt tgaggtgctg ctgttgtggc acgttgactt
     6001 gcacgttcac gttcttgttc gtgaatttgt gctttcatca tggtacgtgt tacatcaaac
     6061 tcgatagcac caatcatatc ttggaacatc ttgaaacctt ctgcttggta ttccaccaca
     6121 gggttatttt gagcataccc tcgaagtcca acagcattac gaagttgatc taaggcatca
     6181 atgtgttcag tccatttgtt atccacaatc atcaaaatga gtactttttg gaattcaata
     6241 atagcttctt gatcacgaag ttttgatagt tgctggtcgt agatagcaag cgctctttga
     6301 taaagcttct ctttgatttg ttcgtctttt aaaccacgta actctttagc actgatagac
     6361 tcttctggaa caagtgacgt gcgtgcaaag gtcacaatgg catccacagc atccttacga
     6421 ttgctacgtg catgagcatc tacagcacgg tcgatagtac gcttgatcat agctttaatt
     6481 tctgggccaa gatctcgatt agcagttatg acatcacgac ggttagcata gataatttca
     6541 cgctgttcac gcatcacatc atcgtattgt aagacctgtt tacgggtatc gtagttattt
     6601 ccttcaaccc gtttttgagc agattccact tgacgcccta acatacctga tttaataaca
     6661 gtgtcttctt catctagttt catccgatcc aaaaaggctt ttatacgatc agagccgaaa
     6721 cgtctcatca aatcatcttc taatgaaaga tagaattggg attcacctgg atctccttga
     6781 cgacctgaac gtccacgaag ctggttatcg atacgacggc tttcatgacg ctctgtacca
     6841 atgacacata acccaccaag ttcacgaaca ccttcaccaa gcttgatatc agtaccacga
     6901 ccagccatgt tagttgcgat tgtaacagca ccacgttgcc cagcattcat aatgatttga
     6961 gcttctttaa agtgattttt agcattcaaa acctcatgag gaatccctgc ttcaaccagc
     7021 ttgcgtgaaa tcaaatcact agtttcaaca gcaaccgtac ctacaaggat aggttgtcct
     7081 tttgcatgac gcgttttaac gtcttccaca actgctctaa actttgattc taaggtcggg
     7141 taaagcaaat cagtgtgatc aatacgggca attggacgat tggttggaat tggaataata
     7201 cgcatgttat aaacttcgcg aaattcttct tcctcggttt tcgcagtacc agtcatacct
     7261 gccaattttt tgtacatacg aaacatattt tggtaagtga ttgaggcgct ggtttttgat
     7321 tcttcctgaa tacgaacgcc ttcttttgcc tcaatagctt ggtgcaagcc gtctgagaag
     7381 cgacgccctt ccatcgtacg acctgtaaat tggtcaacaa taagaatctc accatcttcg
     7441 ctgaccacat aatcgatatc taacaacata atatagtttg cacgaagggc gttgtctata
     7501 aagtgagtca aggctacatt ttcaatatcg taaagattgc tcaagttaaa ataactctca
     7561 gctttatcaa tacctgaatc gcttaatcca atcgttttag tgggcacatc aataacataa
     7621 tccactgacg tcagagtttt tacaaacatg tcagcacgaa tatacaattg attggtttca
     7681 gagctaacag caccagaaac aattaaaggt gtccttgcct cgtcaattaa gactgagtcc
     7741 acttcatcaa ccaaggcaaa attgagtggg cgttgtacca tgtcttcctg gcgaacaacc
     7801 atgttgtcac gaaggtaatc aaaaccgact tcagagttgg ttgaataagt aatatcacaa
     7861 ttataagctt cacgtttttc agcaggagac ttagcagcta ggttgatccc aactgaaaga
     7921 cctaaccagc tatacacttc acccatttcg gtagcgtcac gcgttgaaag atactcatta
     7981 acagtaataa cgtgaacacc ttcaccagca atagcattga gataaactgg catggtagct
     8041 gtaagagttt ttccttcacc agtacgcatt tcagggacat ccccgttatg aagaacaatt
     8101 cctcccataa tttgaacacg ataagggaac aagcccaaca cacgttttgc tgcttcacga
     8161 acaactgcga aagcttcagg taatagttgt tcgagggtct cacctttttg atagcgttct
     8221 ttaaattcaa gtgtttttcc ttgtagatct ctatccgaca atgacgccat ttggtctgcg
     8281 taggattcta ctttttttgc aattttttct aattttctta gttcgccttt gtcattttcg
     8341 ataactttgc gtagaatatt ggccattctc tttcctttcg atcatctaat catttcattt
     8401 taacataaat atgcaggcag ttcaagaaag gttggtaaag ttatcacgcc gtacgcatga
     8461 aa
//
LOCUS       DAUELZ010000072         8277 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.073, whole genome shotgun sequence.
ACCESSION   DAUELZ010000072 DAUELZ010000000
VERSION     DAUELZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.073"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            192..2192
                     /gene="metG"
                     /locus_tag="VHI09_001265"
     CDS             192..2192
                     /gene="metG"
                     /locus_tag="VHI09_001265"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEQ6121141.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
                     EVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGT
                     AFDADLSQLIDAQLADYHKHMEAVDYPRALEAIWTIIARTNKYIDETAPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVSDLSTLALADFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVA
                     NLKPRKMMKQYISQGMILSAEHGDQLTVLTVDPSVPNGSIIG"
     gene            2687..3700
                     /gene="nrdF"
                     /locus_tag="VHI09_001266"
     CDS             2687..3700
                     /gene="nrdF"
                     /locus_tag="VHI09_001266"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ6121142.1"
                     /translation="MTQYYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRARVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYESPFTEEETRVSPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            3704..4138
                     /gene="nrdI"
                     /locus_tag="VHI09_001267"
     CDS             3704..4138
                     /gene="nrdI"
                     /locus_tag="VHI09_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ6121143.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQNKPTT"
     gene            4158..6338
                     /gene="nrdE"
                     /locus_tag="VHI09_001268"
     CDS             4158..6338
                     /gene="nrdE"
                     /locus_tag="VHI09_001268"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ6121144.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKHYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRLSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECHSCMI"
     gene            complement(6544..7296)
                     /locus_tag="VHI09_001269"
     CDS             complement(6544..7296)
                     /locus_tag="VHI09_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEQ6121145.1"
                     /translation="MLKKRYQLAMILLLSCFSLIWQTEGLVELFICEHYERAVCEGTP
                     AYFTFSDQKGAATLIKKRWGKGLVYPRTEQEAMAAYTCQQASLINTSLDKTKGELSQL
                     TPELKDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADLTSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDHKKLHIEACFKGSL"
     gene            7755..8249
                     /locus_tag="VHI09_001270"
     CDS             7755..8249
                     /locus_tag="VHI09_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121146.1"
                     /translation="MKTKKSKRFLNLATLCLALLGTTLLMGRPVKAEGSVVSDNSSGS
                     TSSLVDDSERDKIGRQYEAGDLTLPYNKGRYDGYIDGYEDGKKQGALHPDPTGDHLNK
                     LPKTIPYKFNDRSQKMNYEGGYSDSYPAGYYNGWADNNKIQATLEWLWGEISNWFSSI
                     FTTE"
BASE COUNT         2416 a         1849 c         1757 g         2255 t
ORIGIN      
        1 cctcctttga ttgactggtt ttgctgcccc ttaaaatatg atataataga ccattgatag
       61 ctgttactta acagaaggct gataaaaaag ttccattagc cttgagggtt gaatttaaac
      121 agggaaaaac gaaatcctac actatgcatc tcaatcgtac tttcatttga gtatggttct
      181 gaaaggaaac tatgatgaaa aaaccatttt acattacgac acccatttat tacccatctg
      241 ggaaattaca cattggttct gcttatacaa ctattgcttg tgatgtcttg gcacgctaca
      301 agcgcttgat gggccatgag gtcttttacc tcacagggct tgatgagcac ggacaaaaga
      361 ttcaaaccaa ggcaaaagaa gctggtatta cacctcaaac ctatgttgat aacatggcaa
      421 aggatgtcaa agccctttgg caattgctag atatttccta tgacacattt atccggacaa
      481 cggacgatta ccacgaagag gtcgttgctg ctgtctttga aaaattgctg gcgcaagacg
      541 acatttacct tggcgaatac tctggttggt attctgtctc tgatgaggaa tttttcactg
      601 aaagccaatt aaaagaagtc tttagagatg aagacggtca ggttattgga gggattgccc
      661 catcaggtca tgaggtcgag tgggtctctg aagaatccta cttcctacgc cttagcaaat
      721 acgctgatcg tcttgtggcc ttctttaaag agcgacctga ctttatccaa ccagatggtc
      781 gtatgaatga aatggtgaaa aactttatcg agccaggcct agaagatttg gctgttagcc
      841 gtactacctt tacatggggt gtacctgttc catcagaccc aaaacacgtg gtttatgtgt
      901 ggattgatgc cctcttaaat tacgcgacag ctcttggtta tggtcaagct aatcacgcta
      961 actttgataa attctggaat ggaacggttt tccacatggt tggaaaagat atcttacgtt
     1021 ttcatagcat ctactggcca atcttactca tgatgcttga tttaccaatg ccagaccgct
     1081 taattgctca tggttggttt gtcatgaaag acggtaaaat gtctaaatca aaaggcaatg
     1141 tggtctatcc agaaatgtta gtggagcgct ttggacttga tcctcttcgt tattacctca
     1201 tgcgttcttt gccagttggt tcagacggaa cctttacccc tgaagattat gtgggtcgta
     1261 tcaactatga gttagccaac gaccttggta atttattgaa ccgtaccgtt gccatgatca
     1321 acaagtattt tgatggcacg gtgccagctt atgttgacaa tggaactgcc tttgatgctg
     1381 acttaagtca actgattgat gctcagctag cagactacca taagcacatg gaagcggtgg
     1441 actacccacg cgccttagaa gccatttgga ccatcattgc tcgtaccaac aaatacattg
     1501 atgagacagc tccttgggtg ctcgctaaag aagatggtga caaggctcaa ctagccagtg
     1561 tcatggcaca cttggcagct agcttgcgcg tggtcgctca tgtgatccaa ccctttatga
     1621 tggaaacatc agctgctatc atggcccagc ttggcttagc gcctgtttct gacttgtcaa
     1681 cacttgctct agcagatttt cctgccaata caaaggtcgt tgccaaaggc acaccgattt
     1741 tcccacgtct tgatatggaa gctgagattg actacatcaa agcccaaatg ggtgacagct
     1801 cagctatttc ccaagaaaaa gaatgggtcc ctgaagaggt tgcgctcaaa tctgaaaaag
     1861 acgtcatcac atttgaaacc tttgatgctg tggaaatccg tgtggcagaa gtcaaggaag
     1921 tgtcaaaagt ggaaggttca gaaaaattgc tgcgctttag agtagatgca ggagacgggc
     1981 aagaccgtca aatcttgtca ggtattgcca aattttaccc caacgaacaa gaattagtgg
     2041 gcaaaaaact acaaattgtt gctaacctga aaccgcgtaa aatgatgaaa caatacatca
     2101 gccaaggcat gattttatca gcagagcatg gagatcagct aacggtctta accgttgatc
     2161 cctctgttcc aaatggaagt atcattggct gatgctgacc tattattcaa aaaacctaag
     2221 caagaacatg tgcttaggtt tttttgattg ccttcaagaa agacgaataa cgagatgggt
     2281 gatatgacat caaaagcata actatcctag gacctgtcct agtaagattg ctgtgctttt
     2341 gacaagtgaa cagagaggtc ttaaaactat atcttgtcat aaaaaagatt gcaaaacact
     2401 atatcttgtg ttattatggt ttttataaaa gttaagcatg cttaacttta gccaaacaaa
     2461 agaggacact agagcgtgtc ccacaagaca gttgagccct tcgctttttt agtagagtag
     2521 gagtgctcaa gtcaaaaaga gacatcccat caaaccattg ctgtttagca ggtctttttg
     2581 tgttgctcaa aaaaacagtt cctgtctgtt tggcgtctag tgatgactta ggcttagcgc
     2641 tactagactt ttgtttgaaa aatacctcaa gccttgaagg agacaaatga cccaatatta
     2701 ttatgaacgt tcgcaatccc caattgaata cgccctatct gaaacacaaa aacagttgcg
     2761 ctccatcaat tggaattacc ttaatgatga caaagatttg gaagtgtgga accgtgtcac
     2821 tcaaaatttt tggttgcctg aaaaggttcc tgtttctaat gatttaaatt cttggcgctc
     2881 cttgggtgag gattggcaac agctcatcac aaggacctac actgggttga ccttactaga
     2941 tacagtacaa gcaacggttg gtgatgtggc acaaattcag cattcacaaa ccgaccacga
     3001 acaagtcatc tatacgaact ttgcctttat ggtggggatt catgcccgct cttacgggac
     3061 gattttttcg accctttgca gcagtgagca aatcgaagaa gcacatgaat gggtggtctc
     3121 aacacagagc ttgcaagacc gtgcccgtgt cttgattcct tactatacgg gtgatgaccc
     3181 tttgaaatca aaagtagcag cagctatgat gccaggattt ttgctgtatg gcggctttta
     3241 ccttcctttt tacctatcag cacgtggtaa aatgccaaac acctctgata tcatccgtct
     3301 gattttacgc gacaaagtta ttcacaacta ttacagcggt tacaaatacc aacaaaaagt
     3361 agcacgtctt agcccagaaa aacaggctga aatgaaggcc tttgtctttg acttgctcta
     3421 tgaactcatc gaccttgaaa aagcttactt gagagaactg tatgcaggct ttgatttggc
     3481 agaagatgct atccgcttta gcctttataa cgctggaaaa ttccttcaaa atttgggata
     3541 tgagtcgcca tttacagaag aagaaacgcg cgtttcacca gaagtatttg ctcagctatc
     3601 agcgcgtgca gacgaaaacc atgacttttt ctcaggcaat ggctcatctt atgtgatggg
     3661 gatcaccgaa gaaaccacag atgacgattg ggagttttaa gaaatggcag agctgattat
     3721 tgtgtatttt tcatctaagt ccaataacac ccatcgcttc gtccaaaaat taggcctgcc
     3781 tgctcagcgc ataccagtag ataataggcc acttgaagtt tcaacccatt accttttgat
     3841 tgtgcctacc tatgcagctg gtggctcaga tgccaaagga gccgttccta aacaagtgat
     3901 tcgttttttg aataatccta acaatcgtaa acattgtaag ggagtgatct cttcaggaaa
     3961 taccaatttt ggcgatacct ttgccttagc aggccctatc atttctcaaa aattgcaggt
     4021 ccccttatta caccaatttg aactattagg cactgctaca gatgttaaaa aggtacaggc
     4081 gatttttgcc cgcttaaagc atcacacaca cgacaagcaa aacaaaccaa caacctgatt
     4141 acagaaagga cacacccatg tcacaaacca atgcgtcata cctctcatta aatgcactga
     4201 ctcgttttaa aaaaccagac ggcagctacc attttgatag cgataaagaa gctgttaggc
     4261 gctacctgga agaacatgtc tctcccaatc aaatggcttt taactcacta gaagataagc
     4321 tagcttacct catcaatgaa gggtattatg agcaggccat ttttgatgcc tatcctaacg
     4381 accttatcaa ggaggccttt cattatgcct atcaacaagg ctatcgcttt ttaaatctca
     4441 tgggcgctat gaaattttac cagtcctatg ccttaaaaac cttggacggt aagcattacc
     4501 tagaaacatt tgaagaccgt gctgtgatga atgcactctt tttagctgat ggagatcaaa
     4561 cctttgtctt tgacgtgatt gatgccatct tgcaccgacg ctttcaacca gctaccccaa
     4621 cctttttaaa tgccggaaaa aaacgccgtg gcgaatacat ttcgtgctat cttcttcgtg
     4681 tcgaagacaa tatggaatct atctctcgcg ccattagcac cagcttgcaa ctctcaaaaa
     4741 gaggaggcgg agtcgccttg tgcctcacca acctcagaga aatcggagca ccgataaaag
     4801 ggattgaaaa tcaggcaaca ggtatcgttc ctgtcatgaa actactagag gactcttttt
     4861 cctatgccaa tcaactaggt cagcgccaag gagcaggtgc ggtttacctt catgcccatc
     4921 accccgaggt cctgaccttt ttagacacca aacgtgaaaa tgccgatgag aaaattcgca
     4981 ttaagtccct agcattggga cttgtgatcc cagatattac cttccagtta gccaaagaaa
     5041 acaaagacat ggctctcttt tcaccctatg atattaagcg tgcctatggc aaggacatgt
     5101 cagacatctc catcacagag gaatacgata aactgctagc caatccggcc atcaaaaaga
     5161 cctacatcag tgctagaaag ttctttcagt taattgcaga gctacatttt gagtcaggct
     5221 acccttacct cttgtttgat gacacggtca ataagcgcaa ccctcatgcc aaaaaaggca
     5281 gaatcgtcat gtctaacctg tgttctgaaa tcgctcaagt cagcacccca agcactttca
     5341 aagaagattt gagttttgaa accattggag aggatatctg ctgtaattta gggtctatca
     5401 atattgccca ggctatggca gatgcgccac actttgaaca gctcatcaca acgagcatca
     5461 gagcacttga tcgtgtctct agactgtctg acctgaattg tgcgccttct gttgagacag
     5521 gcaatgcagc caaccatgca gtaggattag gcgccatgaa cctacatggg tttttggcaa
     5581 ccaaccacat ctactatgat acaaaagaag ccgttgactt tacagatctg tttttccatg
     5641 ccatggccta ttatgctttt aaagcctctt gccaactagc caaagaaaaa ggcgcttttg
     5701 caggattttc gttatcaacc tatgcagatg gcacttattt tgccaagtat ttgcaagaag
     5761 atgccaagcc acaaacagcc aaggttgcca cattgttgca agattacggc ttcacattgc
     5821 caacggtggc ggattggcaa gctctagtag cagatatcaa gcagtttggc ctagccaatg
     5881 cgcacttgct agcggttgct ccgacaggct ccatcagcta cttatcatca tgcactccga
     5941 gtctacaacc agtcgttgct cctgttgagg tccgaaaaga aggtagcctg ggcaggattt
     6001 acgtccctgc ctatcagatt gaccaggcta actatgctta ctatgaacgt ggtgcctatg
     6061 aggtcggccc aaaagccatc atcgacgttg tcgctgctgc ccaaaaacac gttgaccaag
     6121 ccatctcatt gaccctgttt atgaccgatc aagcgaccac acgtgacctt aaccgctcct
     6181 atatccaagc ttttaagcaa aactgtgcat cgatctatta tgtgcgagtg aggcaagacg
     6241 tcctagcagg cagcgaacag tatgacgagg atagtcttgt gacagctcct ggagctagtg
     6301 atgagaccac cacagagtgt catagctgta tgatctaaca gctactagaa agctctcgtg
     6361 tcccctacca catgcatagg atcagtgcgc tcgacctttc aagacaagca agcatcagct
     6421 cttgcttgtc tttttttggc ctcaaagccc gttagtctgc tgctatgcga ggcttttttt
     6481 gagcatcaga acctcaaaaa aaaggacatg gagtcctttt tggggtgatc ggtgttgagg
     6541 ccgtcacaaa ctgcccttga agcacgcttc tatgtggagc tttttgtggt cctgtgacac
     6601 gtaagctcca acgacctcca gctgacagcc tcttggaaac aagagctcaa cctctgaagg
     6661 cacagccgaa taaggctcga caaaggctgc tttggcccct tttttgacac agatgcgcac
     6721 ctccaccggt cgatgggtca tagcgccgtt tttcaaggct gtcgtgctca taaaactgtg
     6781 cttagtgtag cgtgtgccaa gcttgatctt acaaaggata tgagggtcaa actgatggtt
     6841 acgatagtaa gacgtgagat cagcgtgtga aacaccgata tcacgcaaaa acgtctcgta
     6901 tacatagcgg tataccacaa tgttccacgg gatgactagc cggtgagtcg cagcatcgag
     6961 ctgggccacc tgatccttta gctcaggcgt gagttggctg agctcacctt tggtcttgtc
     7021 taggctggta ttgataaggc ttgcctgctg acaggtataa gcagccatcg cctcttgctc
     7081 agtccttggg tagacaagac ccttgcccca tcgcttttta attagtgtcg cagcgccctt
     7141 ttgatccgaa aaggtaaaat aagcaggcgt cccctcacaa accgcccgct catagtgctc
     7201 acagataaaa agctcgacca agccctcagt ttgccagatc agactaaaac agctaagaag
     7261 aaggatcata gcgagttgat agcgcttttt tagcatagtt ccccctgaca ccagcccaaa
     7321 aggctgcccc ttcccctaga gcttttggct agctcttgac accaccagtt tctcagacag
     7381 gctaatcttt ttgtcatctc tttctcccta aacagactct cttatcatga tattatgcta
     7441 ttatgagcga tttgtcaatc catagcaacc gttttttagc cagataagca agcttttgac
     7501 ctataggcag acaagagtgg cagtacctac cgttcactgt ggtggctgat aagaactaac
     7561 ataaaatctc aaacgctttc aaaaacagct cctgagctta aaaaaggctg atgaacaatt
     7621 tttttgagtt taagagagat tgccataatt ttctaatcat ttgatgatta aaatgataaa
     7681 aacatgaact tgaaatgaag gagtgcgagt agtaaactat aatcgtatca aaaataatga
     7741 aatggggtaa ttttatgaaa acaaaaaaat caaaacgctt tttaaaccta gcaacccttt
     7801 gcttggccct actaggaacg actttgctaa tgggacgtcc cgtaaaggcg gagggaagtg
     7861 tagtttcaga caattcatcc ggatccactt cttcgctagt ggatgatagt gaaagagata
     7921 agattggtag acaatatgaa gcaggagatc taacattgcc ttataataaa gggagatatg
     7981 atggttatat agacggctac gaagatggta aaaaacaagg agccctgcat ccggatccta
     8041 ctggtgatca cttaaataag ttacctaaaa ctattcctta taagtttaat gatagaagtc
     8101 agaaaatgaa ttatgagggt ggctatagtg atagttatcc tgcaggatac tataatggtt
     8161 gggctgataa taacaaaatt caagcgactt tagaatggtt atggggtgaa ataagcaatt
     8221 ggttttctag tatatttaca actgaatagt ctatcatctt actaaagcga ttttcat
//
LOCUS       DAUELZ010000073         8096 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.074, whole genome shotgun sequence.
ACCESSION   DAUELZ010000073 DAUELZ010000000
VERSION     DAUELZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.074"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            12..710
                     /locus_tag="VHI09_001271"
     CDS             12..710
                     /locus_tag="VHI09_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6121147.1"
                     /translation="MFSGHQLKTARLSKGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQINKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
     gene            complement(827..2389)
                     /gene="ffh"
                     /locus_tag="VHI09_001272"
     CDS             complement(827..2389)
                     /gene="ffh"
                     /locus_tag="VHI09_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEQ6121148.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKFDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMAKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLNGDMDMSQLFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(2404..2745)
                     /locus_tag="VHI09_001273"
     CDS             complement(2404..2745)
                     /locus_tag="VHI09_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEQ6121149.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            complement(2834..3532)
                     /locus_tag="VHI09_001274"
     CDS             complement(2834..3532)
                     /locus_tag="VHI09_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ6121150.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEYFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            complement(3615..5048)
                     /locus_tag="VHI09_001275"
     CDS             complement(3615..5048)
                     /locus_tag="VHI09_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121151.1"
                     /translation="MLSKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKEPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            5215..6777
                     /gene="guaA"
                     /locus_tag="VHI09_001276"
     CDS             5215..6777
                     /gene="guaA"
                     /locus_tag="VHI09_001276"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEQ6121152.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            complement(6819..8042)
                     /locus_tag="VHI09_001277"
     CDS             complement(6819..8042)
                     /locus_tag="VHI09_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ6121153.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLILINQDITRTQAHQKKRLKKL
                     EDQKASLERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPKVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYTIRKQLKNKH"
BASE COUNT         2419 a         1550 c         1449 g         2678 t
ORIGIN      
        1 ggagctacca aatgttttct ggacatcaac taaaaacagc acgattatca aagggaataa
       61 ctcaatcaga attgggaaga ttgttgcatg tcaataaaat gacaatatct aattgggaaa
      121 aaggtaagaa tataccaaat gaaaaacatt taaatgcctt attgcatcta ttcaatgtga
      181 catctgatta tttcgaccca aactatagat tgctaacgcc ttataaccag ctgacaatat
      241 ctaataaaga aaaagtaatt ggctattcag agcgattgtt aaatcatcaa ataaacaaaa
      301 aatctaaaga tctcatagat aaaccatcac aattatatgc ttatcgggtc tatgaaagtt
      361 tatctgctgg tactggttac tcctattttg gtgatggtaa ctttgatgtt gtcttttacg
      421 atgaacaatt agaatacgat tttgcgtctt gggtttttgg agattctatg gagccaactt
      481 atttaaatgg tgaagttgtt cttataaaac aaaatagttt tgattacgat ggagcaattt
      541 atgcagtcga atgggatggg caaacatata tcaaaaaggt atttcgtgaa gatgagggat
      601 tacgtctagt gtccttaaat aaaaaatatt ctgataagtt tgctccctat agcgaggaac
      661 ctcgcattat tggcaaaatt atcgctaatt ttaggccctt agaaatttaa gttagattga
      721 taatgaactc atcatggtat gtaacacggt cttccgtaat tttaataatc ttgtatacaa
      781 gataaatgaa tttcacatca aaaagagacg aaaattttcg tctcttttat tttcgttttt
      841 tctttgcttt ttttagtttg ttagcctgcc gtttcatggc ctgtttcatg gcaaattggc
      901 caatttttcc tttaaaccct ttaccaaata gttgactcat atccatatca ccattaaggc
      961 cagataaatc aggcatacca ccttgtccca tcatcccttc taaggaagac atatctggca
     1021 taccagcagg catgttttta ggaagattat tgggattaat acccatatct ttcatcattt
     1081 tagccatatc gcccgacata accccttgca tcatgctttt ggcttggtta aagtctttga
     1141 taaatttatt aacttccaca aagctgttcc cggaaccagc tgcaatacga cggcgacgac
     1201 tcgggtttag taagtcagga ttttcacgtt cagcgggtgt cattgaagag acaatagcac
     1261 gtttgcgagc aatttggttt tcgtcaaatt tgatattggc caaagctggg ttaccagcca
     1321 ttcctggaat cattttcaag aggtcttcca taggccccat attttgcact tgatctaact
     1381 gttcaataaa atcattgaaa tcaaaggtgt tctcacgcat tttttcagct aactctaagg
     1441 attttttctc atcatattcc tggctagctt tttcaatgag ggtaagcaaa tcccccattc
     1501 ccaaaatccg actggacatg cggtctgggt gaaatgtttc aatatctgtg attttttcac
     1561 caatacctgt aaatttaatt ggtttcccag tgatttcacg gacagatagc gctgcacctc
     1621 cacgggtatc accatcaatc tttgttaaca caacccctgt gatgctcaac tgatgattaa
     1681 actcgtaggc tacattggca gcttcttgac caatcatgct atctacaacc aaaagaattt
     1741 catttggttg tgctaaagcc ttgacatcgc gcagctcttc catcagtttt tcatcaattt
     1801 gcaagcgacc cgcagtatca atcaagacat agtcattgcg gttttcacga gcttgttcca
     1861 aaccttttct gacaatatcg actgctgagt ggtctgtccc catatcaaaa acaggtacat
     1921 tgatttgttg ccccaatgtt tttaactgat caatggccgc tggacggtaa atatcggcag
     1981 caatcatcag tggtcgcgcg ttttcttctt taattaattt attagcgagt tttcctgcaa
     2041 aagttgtttt accagcccct tgcaaaccga ccatcatgat aatagttgga attttgggag
     2101 atttatcaat ctctgcagtt tctgagccta aaatactcgt taattcttca ttaacaatct
     2161 taagaatttg ttgtgttgga tcaagagtat caataatctc atggccaata gcgcgctcac
     2221 gtacgcgttt aataaaggtt ttaacaactg gtaaagcgac atcggcttct aaaagagcta
     2281 gacgaatctc ttttgtcact tcttgaacat ctgattctga taatttcttt ttaccgcgaa
     2341 tgtgtttaaa cacatcttgc aaacgttggg ttaagctttc aaaagccata gtcaaaactc
     2401 ctcttactct ctattgtcaa tactggttag aatggaaatt ttttcttgca aatactcatc
     2461 atgagggtag tgagcaatca tgtcgtcaaa gatttcactt cgaacaacat aatccgaata
     2521 catatgaagt ttcatctcat aagtctctaa aattttttcc gtacgcttaa tattatcata
     2581 gacagcctga cgactgacac caaattcatc agcaatctca gctaagctat aatcatcggc
     2641 ataatacagt tcaatataat tcatttgttt atctgttagc aaagctgcat aaaattcaaa
     2701 aagggcattc atcctgtttg ttttttcaat ttccataatg ttcattttat catactatga
     2761 ttgtttttaa cagtagcaaa ttaattgcct tgtgctgttc gtgtaaaaaa agaagtcata
     2821 aatgacttct ttactaatta ttttctaagt aaaattcaaa acggtcaccg atatactgac
     2881 tgcggacata ctcaaaaggt ttgccgtctg taaagtaaga cacctgagtt agagccaaaa
     2941 tggcatgtcc tttagcaact tccaagtagg aggctacacg ctcacttgct agcttagcgt
     3001 agattgtctg tttactttta ccaatctcgt aaccatttgc tatcaatgag tgaaaaaagt
     3061 gttctgtaat atcagctcgt ttaaccgttt ttataaattt ttctgggatt gaagcaattt
     3121 cgtacaccaa agggacactg tctgcatagc ggatacgttc cattcgaatg accaagtctg
     3181 ttttgtctaa gttcaattct ttaacttcgg tatcactagc taattgcttt tgataggata
     3241 ataatttaga ggagggttgt cgtccttggg agcgtataat ttctgtgaaa cttgttgttc
     3301 ctcgcatttt ttcttgaaca cgatgactgg caacataagt accacttcct atccgccttt
     3361 caagaatccc ttcttctact agtaatgtaa tagcctgccg tagagtcatt cgactaacgg
     3421 tgaaatattc cgctaaatgt cgttcacttg gcaagcgact accaattggc cagatgccca
     3481 gatcaatgtc ttttttaatt gcatcatgaa tttttatata agctggtaac atgccacact
     3541 cctcaagtcg gttattcatc tattttatca tatttttgat gaaaattgaa ttgtaaatat
     3601 tgaggtattt ggcatcaatt aatgatatta cctgcgacaa taaccggtga atttggtgga
     3661 aaatgaatag gttccataaa ccaactagtg agcttcgctt tagtaacaat ggatgaggta
     3721 aaaacattat ttaaaaccct aataactcct acttttgagg aatgctgacc aatagaacca
     3781 ctataggctc tcaggcaacc agtcggatct atataaggta aaaattgatt ttggcttaca
     3841 gtaatattgt gacatagatg tcgagtttgg ttaaatgctt catagtttgg agcaatatta
     3901 ctaagtagac ctccatccca tgcaaaatga tgaacagcag catcaaactg aatgacttct
     3961 gaaaaaaaat catgcccttg agcctttgat agcagctgtt ggtcttgtac taattctggc
     4021 gcatagccaa taaattggtt tttctcaaac aatgaatttt gtagagatcc taaatcaaaa
     4081 atatgactat tacgcttatg aaccatagta aatttgttat cgtaaatatg ccaatcagta
     4141 ccatgatcag ccataatcat aaagtggtct ccttttttca aatcatttgc tttgaaatgc
     4201 actccagtca acacgagatt acgaacacct tgttcagcct ttggtcctgt agggaatcca
     4261 aaccacaaca ttttaccgtg aataatgaac tctgtctgat gaccaataat agctgtatca
     4321 gatggaagct caaaataatc tttttgtgaa ggcaaagagc caatgtgaaa ttgccccttt
     4381 ggaaaagata taacgctatt tggatgctgc tttgcataag cgaaagcttt tcgcaaaaga
     4441 cgattgtttt gctcagggga atttccttta ttgaacctaa tataataagg tttaacaaaa
     4501 tcttgctctg aaattccatc taatcttaga ttatctttca gattaatatc gtactgatat
     4561 cctcctaacg aatagtcata gcgtttttta acttgtttaa tagtcgctaa ttgaccttga
     4621 tatactttta tatgtggctt tttagttaat gaagataatt taacgatatc tcctttttta
     4681 aacctcggtt tatcgggctc tttcaggtta tgttctggca aagaaacagt gacttttcct
     4741 attggaaata ccacttcata atgataactc actcggtaat tttcggctat atcttctacc
     4801 tgtttattaa tagttccaat ccattgatga tatggaccac ttttttgtac ttgaatagct
     4861 tgtccaatgt gatagcgagg ctgttgatct ttgcttcgtt ccactattcc gacagttgct
     4921 aatatattgt gaagtgttgc aaaactattg gtccacgcaa ggtacgagac aactaaactc
     4981 aacaataatc ctaggaaaat taattcaaca ggtcgaaata aacccaaaat tctctttttg
     5041 cttagcatag gccctccctg ttgtcttaat tccctttcat agcctctatt gtaacataac
     5101 gattacgttt ttttccaacg agctagagaa attgtaatgt cttttcgatg aaaatatgct
     5161 acaataagtt gacaactatc aacctgacgg ttggtataga atgaaaggat tataatgact
     5221 gaaatttcaa ttttgaatga tgttcaaaaa attatcgttc ttgattatgg tagccagtac
     5281 aatcagctta ttgctagacg tattcgagag tttggtgttt tctccgaact aaaaagccat
     5341 aaaatcaccg ctcaagaact tcgtgagatc aatcccatag gtatcgtttt atcaggaggg
     5401 cctaactctg tttacgctga taacgccttt ggcattgacc ctgaaatctt tgaactaggg
     5461 attccgattc ttggtatctg ttacggtatg caattaatca cccataaatt aggtggtaaa
     5521 gttgttcctg ctggacaagc tggtaatcgt gaatacggtc agtcaaccct tcatcttcgt
     5581 gaaacgtcaa aattattttc aggcacacct caagaacaac tcgttttaat gagccatggt
     5641 gatgctgtta ctgaaattcc agaaggtttc caccttgttg gagactcaaa tgactgtccc
     5701 tatgcagcta ttgaaaatac tgagaaaaac ctttacggta ttcagttcca cccagaagta
     5761 agacactctg tttatggaaa tgacattctt aaaaactttg ctatatcaat ttgtggcgcg
     5821 cgtggtgatt ggtcaatgga taattttatt gacatggaaa ttgctaaaat tcgtgaaact
     5881 gtaggcgatc gtaaagttct tctaggtctt tctggtggag ttgattcttc agttgttggt
     5941 gttctacttc aaaaagctat cggtgaccaa ttaacttgta ttttcgttga tcacggtctt
     6001 cttcgtaaag acgagggcga tcaagttatg ggaatgcttg ggggcaaatt tggcctaaat
     6061 attatccgtg tggatgcttc aaaacgtttc ttagaccttc ttgcagacgt tgaagatcct
     6121 gagaaaaaac gtaaaattat tggtaatgaa tttgtctatg tttttgatga tgaagccagc
     6181 aaattaaaag gtgttgactt ccttgcccaa ggaacacttt atactgatat cattgagtca
     6241 ggaacagaaa ctgctcaaac catcaaatca catcacaatg tgggtggtct ccccgaagac
     6301 atgcagtttg aattgattga gcccttaaac actcttttca aagatgaagt tcgagcgctt
     6361 ggaatcgctc ttggaatgcc tgaagaaatt gtttggcgcc aaccatttcc aggtcctgga
     6421 cttgctatcc gtgtcatggg agcaattact gaagaaaaac ttgaaaccgt tcgcgaatca
     6481 gacgctatcc ttcgtgaaga aattgctaag gctggacttg atcgtgacgt gtggcaatac
     6541 tttacagtta acacaggtgt ccgttctgta ggcgtcatgg gagatggtcg tacttatgat
     6601 tataccatcg ccattcgtgc tattacgtct attgatggta tgacagctga ctttgctcaa
     6661 cttccttggg atgtcttgaa aaaaatctca acacgtatcg taaatgaagt tgaccacgtt
     6721 aaccgtatcg tctacgacat cacaagtaaa ccacccgcaa cagttgaatg ggaataacag
     6781 aataaacatt aaaagactcg ctcattttgg cgagtctttt aatgtttatt ctttaactgt
     6841 tttcgtattg tgtacatggt attagcaata tgataaagtg ggctgacagg gagattaaat
     6901 tctcctataa attcttctat tgtcggagca aagttggcct taaatttggt aagtccatca
     6961 tcaagcgagc cttctactcc tcccatgtta gcccaaataa taccatcttg ataagcatcc
     7021 tgaaaaactt taggatacag cagatactga ggataaaact tcttaaaatc atcattcatc
     7081 ccagcataaa gcatttccat aacatttcca taagaaatag ataatatacc tgctacaaca
     7141 acttcctcag gatattggtc tgtaaagcct tcaaattcag ttatataacg ttctaaagaa
     7201 gctttttgat cttctaattt ttttaaacgc ttcttttgat gagcttgggt gcgagtaata
     7261 tcttggttaa taagaattaa ttgctggcgg tattgatcta gtttttgagg aatattcact
     7321 tttgctagat gtaagtaggc cttatcccca taagtagtca tcaacttttg aaagtaagct
     7381 tcgttacgca aagaaatatt tttacgtttt tctgttaagg agacaatctt ggaaaatttg
     7441 tgaagttctg attgactggc acgatatgtt tctacaccac gctgcttagc atcttttatt
     7501 aaacgtctgg tatgcttagg aaattgcatc tcaaggtttt cttgagtgta aatattagct
     7561 tggaaacgag gttggatact atctgcaatc tccattgtta aaccagtcca atgaacacca
     7621 gcttcttgga gattcttaat agctgctaaa gctaaaggtt tttcttcttc ttcctgtccc
     7681 agtgcgtatt gttttaacag gatagctgga tcatatttga taaagagggc ctttttagtt
     7741 ttaccataat cctttagtgt tttcatggta aatgccacca aatcacggtc aaggtaatcc
     7801 atgactggcc ctcttggaat ataaataatg cttcttccaa gtggcaatag cttaatcaaa
     7861 agtgataaag aggcaacctg cttttcctct ttatagatac caattcgctc attctgccat
     7921 tgatttttga ttttcgccca atcactacct tgtaaaacgc taatttgctg atgttcttta
     7981 acaaaagagt catgctcttc ttctgaaata ccgatttttg cataaaacgt taaattcgtc
     8041 atatattgtc cccatctatt cgtaatctta gcagatgtac ttactaagta tcttca
//
LOCUS       DAUELZ010000074         7869 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.075, whole genome shotgun sequence.
ACCESSION   DAUELZ010000074 DAUELZ010000000
VERSION     DAUELZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.075"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            42..752
                     /locus_tag="VHI09_001278"
     CDS             42..752
                     /locus_tag="VHI09_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ6121154.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            745..2097
                     /gene="vicK"
                     /locus_tag="VHI09_001279"
     CDS             745..2097
                     /gene="vicK"
                     /locus_tag="VHI09_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEQ6121155.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWLEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            2101..2910
                     /locus_tag="VHI09_001280"
     CDS             2101..2910
                     /locus_tag="VHI09_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ6121156.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            3353..4045
                     /locus_tag="VHI09_001281"
     CDS             3353..4045
                     /locus_tag="VHI09_001281"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEQ6121157.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            4046..7585
                     /gene="smc"
                     /locus_tag="VHI09_001282"
     CDS             4046..7585
                     /gene="smc"
                     /locus_tag="VHI09_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEQ6121158.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFDKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVLSQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKRVQELERDYQLNQERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGANRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKTLLAALDQSQTTHSDESLLAEQARIEEALTAIAKKKNVLTCDIDDIKENKDLIR
                     QKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQ
                     DSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEARLEETAEKIAKESEKNDIF
                     IRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQK
                     TIRALGPINSDAITQYEEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKVT
                     FEAIQKSFKETFTQMFGGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGITMQESGVSKIVSVKLKEAQEMTN"
BASE COUNT         2667 a         1257 c         1642 g         2303 t
ORIGIN      
        1 aaattgtaat atcaattaca agaactttct gaggtgtaga catgaaaaaa atacttattg
       61 tggatgatga aaaaccgatt tctgacatta ttaagtttaa tttgacaaaa gaaggttatg
      121 acattgttac agcttttgat ggacgcgaag cggtaaccat ttttgaagaa gaaaagccag
      181 atttaattat tcttgatttg atgctccctg agttggacgg tcttgaagta gccaaggaaa
      241 ttcgtaaaac cagtcatgtc ccgattatta tgttgtcggc taaagatagt gagtttgaca
      301 aggttattgg acttgaaatt ggggctgatg attacgtgac caagcccttt tctaatcggg
      361 aattgctggc gcgtgtcaag gctcatctgc gtcgtaccga aactattgaa acggctgttg
      421 cagaagaaaa tgcttcttca ggtacacagg aactaaccat tggtaattta cagattttac
      481 cagatgcgtt tgttgctaaa aaacatggtc aagaggtaga gttgactcat cgtgaatttg
      541 aactattgca tcatctagct aaccatatgg gtcaggtgat gacacgagaa cacttattgg
      601 aaattgtttg gggatatgat tattttggcg atgtgcgcac ggttgatgtg actgttcgtc
      661 gtctccgtga aaaaattgaa gacacaccaa gtcgtcctga gtatatttta acaagacgtg
      721 gtgttgggta ctacatgaaa tcttatgact agagatatca ttggaaactt atccacattt
      781 gaattagcga tccttatctt acttgttttt gttgcttttt actttatcca tcttgcggtg
      841 cgtgattacc gaaatgcacg tattattcgg atgatgagcc ataaaatccg agacttgatt
      901 aatggtcgct atactgatat aatcgacgaa aaagcagaca ttgagttaat ggagctttca
      961 gaccagttaa atgacctgtc agatgttttt cgcttgacgc atgaaaatct tgcccaagaa
     1021 aaaaatcgct tggcaagtat tttggcttat atgtcagatg gtgtacttgc tacagaccgg
     1081 tctggtaaaa tcatcatgat taacgagaca gctcgcaagc aattaaattt aagtaaagaa
     1141 gaggcactaa agaaaaacat tacagatttg ttagaaggtg atacttcata tacctaccgt
     1201 gatttggtat ccaaaacacc agtggtaact gttaatagcc gaaatgatat gggtgagttt
     1261 gtctcattac gcttgcgttt tgcgttgaat aggagagaga gtggttttat ttcgggcttg
     1321 gttgtggtgc tccatgacac cacagaacaa gaaaaagaag aacgtgaacg ccgtcttttt
     1381 gtttctaatg taagtcatga attaaggacc cctttaactt cggttaaatc ctacttggag
     1441 gctcttgatg aaggtgcact taaagaagat attgctccaa gtttcataaa agtttctctt
     1501 gatgaaacta atcggatgat gcgtatgatt tcagatcttt taaacctgtc tcggattgat
     1561 aatcaagtaa cccaattagc agtagagatg actaatttta ctgcttttat aacttctatt
     1621 ttaaacagat ttgatttggt taaaaatcag catacaggta caggaaaagt ctatgaaatt
     1681 gtaagagatt accctattac ctctgtctgg cttgaaattg ataatgataa aatgacacag
     1741 gttatcgaga atattttgaa caatgccatt aagtattctc cagatggtgg aaaaattaca
     1801 gtccgtatga aaacaacaga tacccaatta attatttcca tttcagacca aggactaggt
     1861 atccctaaaa cagatttgcc tcttattttt gatcggttct atcgtgtaga caaggcaaga
     1921 agtcgtgccc aaggagggac cggtctaggc cttgccattg ctaaagaaat catcaagcag
     1981 caccatggct ttatctgggc taagagtgac tatggtaaag gatcgacctt tactattgtc
     2041 ttgccttatg aaaaagatgc agccatctat gaagaatggg aggaagatgt agactaatca
     2101 atgaatgaga gtggttttaa atatagtatt ttagcatctg ggtccacagg aaattgtttt
     2161 tatttagaaa cacctaaaaa acgcttattg attgatgcag ggcttactgg taagaaaatt
     2221 accagtcttc ttgctgaaat tgaccgcaaa cctgaggatt tggatgctat tttgattacc
     2281 catgagcact ctgaccatat taagggtgta ggcgttatgg cccgcaaata ccatttggat
     2341 atctatgcta atgaaaaaac atggcagctg atggatgagt gcaatatgct aggtaagctt
     2401 gatgcctcac aaaaacatat tttccaaagg gataaagttt tgacctttgg agatgttgat
     2461 atcgaaagtt ttggagtcag ccatgacgct attgatcctc aattttatcg gattatgaaa
     2521 gataataagt cttttgtgat gttgacagat acaggatatg tcagtgatag gatgacaggt
     2581 attattgaaa atgcagatgg ctacttgatt gaatccaatc atgatattga aattttgaga
     2641 tcaggctctt atccttggag cttgaagcag cgtattttat ctgatttagg acatttatca
     2701 aatgaagatg gagcgggagc catgattaga agcctggggt acaataccaa aaaaatatac
     2761 ctaggtcatc tgagtaaaga aaataacatc aaagagttag cgcatatgac gatggtcaat
     2821 caactggcta tggcagattt agcagtaggt acagacttta cggtccatga tacctctcca
     2881 gatactgctt gtccattaac tgatatttga gataagaaac taaaagagaa tatgcaaagt
     2941 agcgctgatt ggtgatatac cagtcagggc tattttttga tgtcttaaag cgtattgtct
     3001 tctgagttca atgaactctc attggttatc ctttagagca tcaatagact taaattagcc
     3061 tgtagttttg gcttgtctga tctatttatc aaaggtgttg aggattataa agataaggta
     3121 gttttttagt tgttcagact aaaaacaaaa tagctctata ctatctgtag tgggactgta
     3181 gtgggaaatt cccctcagat agtgtagagt cttttgattg tatgagaaag tttcctttga
     3241 cgggtattta agatgtttaa taaatcacga aaaatccact tagagtcggt tttctaggtg
     3301 gatttttggt atactaaata aggtctactg acaaatatga aagggatttt ttatgaaaca
     3361 gcttgaagag ttactctcaa catcttttga tattcaattt aatgatttga ctttactaga
     3421 aacagctttt acccatacct cttacgctaa cgagcatcgt ctcctaaacg tttcacataa
     3481 tgaacgcttg gaatttttag gagacgctgt tctacaattg attatttcag agtatttatt
     3541 tgcaaagtat cctaaaaaga cagaaggaga tatgtctaag ttacgttcca tgattgtacg
     3601 tgaggaaagc ttggcaggtt tttcacgatt ttgttctttt gacgcttata tcaaactagg
     3661 aaaaggtgag gaaaagtcag gtggtcgccg tcgtgatact attttaggag acttatttga
     3721 agccttttta ggtgctcttt tattagataa ggggattgat gcagtacgtc gcttcttgaa
     3781 acaagtcatg attcctcagg tggaaaaagg gaattttgaa agggttaaag actataaaac
     3841 ttgtcttcaa gagtttttgc aaacaaaagg tgatgtggct attgattatc aagtcattag
     3901 tgagaaagga cctgcccacg ctaaacaatt tgaggtatcg atagttgtta atggtgctgt
     3961 ccttagtaaa ggtttaggta agtctaaaaa attagcggag caagatgctg ctaaaaatgc
     4021 ccttgcccaa cttagtgagg tttagatgtt tttaaaagaa attgagttgg aagggttcaa
     4081 gtcctttgcg gacaagacta aaattgaatt tgataaggga gtaactgcag tcgtaggacc
     4141 aaatggttca ggtaaaagta atattacaga aagtttacgt tgggctttag gagaatcaag
     4201 tgctaaaaat ctgcgtgggg gcaagatgcc agatgtcatt tttgctggga cacaaaaccg
     4261 taatcctcta aactatgcta aggtagcggt tgttcttgat aattccgatc attttataaa
     4321 aactgccaag aaagaaatcc gtgtcgaaag gcatatttac cgaaatggag atagtgacta
     4381 tctgattgat ggtcgcaagg taaggctccg tgatattcat gatttgttta tggatacagg
     4441 gcttggtcgt gattctttct ccattatttc tcaaggtcgt gtagaggaaa ttttcaatag
     4501 taaacctgaa gaaaggcgcg ccatttttga agaagcagct ggtgttttaa aatataaaac
     4561 acgtaaaaaa gaaactcaga ttaaattaaa tcaaactcaa gataatttag accgtctaga
     4621 ggatattatt tatgagctag acacgcagtt ggccccactt gaaaagcaag ctaaagtagc
     4681 taaacaattt ttagaacttg atgccaatcg taagcaacta caactagata ttctagtcaa
     4741 agatattgat atagctcaag aaagacagac taaagatact gaagctttag ctgctttaca
     4801 acaagactta gcatcttatt acgctaagcg tcaatctatg gaagaagatt accaaaaatt
     4861 caagcaaaaa aagcaggttc ttagtcaaga gtcagatcaa acccaaacaa cattattaga
     4921 attaaccaag ctaatagctg acttggaaaa gcaaattgaa ttggttaagc tagaaagtgg
     4981 ccaagaagca gagaaaaagg cagaagccaa aaaacatctg gaacagttac aagaacaatt
     5041 ggatggtttt caagcagaag aaaagcagcg tacagaacaa ttattgcata ttgatcaaca
     5101 actttgtgat gtcaaacaac agttaaacga attgagcaat gcattagagc gtttttcaag
     5161 tgatcccgac caattaatgg aaactctaag agaagagttt gtattattga tgcaaaaaga
     5221 ggcggcctta tcaaatcagc tgacagcttt aaaagcccat ttagacaaag aaaaacaggc
     5281 tcgtcaacat aaggctcaag aatatcagct acttgttact aagcttgatc aacttaatga
     5341 cgagtctcaa aaagctcaag ctcattataa agctcaaaaa gagcaagtcg aaatgttgct
     5401 ccaaaattat caagaaggtg ataaacgcgt tcaggaatta gaaagagatt accaactgaa
     5461 tcaagaaaga ctatttgact tgttggatca aaaaaaagga aaagaagctc gtaaggctag
     5521 cttagaatct attcaaaaaa gtcatagtca attctatgcg ggagtacgag ctgtccttca
     5581 atcacaaaaa aagcttggag ggatcatagg tgcagtcagt gaacaccttt cttttgattc
     5641 tgattaccaa acagctcttg aaattgcatt aggtgctaat agtcagcata ttattgtaac
     5701 agacgaagct gcagcaaaaa gagcaattgc ttatttgaaa aaaaatcgcc aaggacgagc
     5761 aactttttta cctttaacta ctataaaggc tagatcatta tcagaacact atcatcgtca
     5821 attggcgact tgtgaaggtt atctgggaac tgcagaatca ttggttcgct atgatgatag
     5881 tctttcagct attatccaaa accttttatc tagtactgct atctttgaaa ctattgatca
     5941 agctaatatt gcagcgcgct tattaggata taaggtaaga attgttactc ttgatggaac
     6001 agaattacga ccaggtggtt ctttttcagg aggagctaat agacaaagca atacaacttt
     6061 tatcaaacca gagttagaac aaatcagtga ggaattaacg cgattagttg agcaattaaa
     6121 aataactgaa aaagaagtag ctgccttgca aagcgatttg atagcaaaaa aagaagaact
     6181 gacacaattg aaattagccg gtgaccaggc acgcttagct gaacaaagag ctcaaatggc
     6241 ttatcagcaa ttgcaagaaa aacaagaaga ttcaaagact ttgttagctg ctcttgatca
     6301 aagtcagacg actcattctg atgaatcttt attggcagaa caagctcgta ttgaggaagc
     6361 attaacagct attgctaaga aaaaaaatgt cttaacttgt gatattgatg atatcaaaga
     6421 aaacaaagat ttgattaggc aaaaaacaca aaacattcac caagctttat ctcaagctcg
     6481 tttgcaagaa agagatttac ttaatgaaaa gaaatttgag caagctaatc aatctcgtct
     6541 aagaactcag ctgaagcaat gccagcaaaa tattctcaaa cttgaaagca tactaaacaa
     6601 caacgtaagt caagacagca ttcaaagact accccagtgg caaaaacagt tacaagatgc
     6661 cacagagcat aagtctggtg ctcaaaaacg tttagtccaa ttaagatttg aaatagagga
     6721 ctatgaggcc cgcttagaag aaacagcaga aaaaatagcc aaagaaagtg aaaaaaatga
     6781 catatttatt cgtcgtcaaa ccaagttaga gacacattta gagcaagtgg ctaatcgctt
     6841 acgagcctat gctaaaagtt tatcagaaga ttttcaaatg acactagcag acgcaaagga
     6901 ggtaacaaat agtattgatc acttagaaag tgccaaagaa aaattacacc atttacaaaa
     6961 gactattcgt gcactaggac caattaatag tgatgctatt actcagtatg aggaagtgca
     7021 tgagcgtttg acttttttaa caagtcaaaa gacagatctg acaaaagcta aaaatctttt
     7081 gctagagact attaatagta tggatagcga agtgaaggct cgtttcaaag tgacttttga
     7141 ggcaattcaa aaaagtttta aggaaacctt tactcagatg tttggtgggg gttctgctga
     7201 tttggtttta acggaaacag acctcttgtc tgctggtata gagatttctg tgcagcctcc
     7261 tggtaagaaa atccaatctc ttaacttgat gtcaggcgga gaaaaagctt tgtcagcact
     7321 ggctttattg tttgctatta tacgtgttaa aacgattccg tttgtgattt tagatgaggt
     7381 agaagcggct cttgatgaag caaatgttaa gcgctttggg gatttcctca atcgctttga
     7441 caaagatagt caatttattg tcgtgaccca tcgtaaagga acaatggctg cagcagatag
     7501 tatttatgga attaccatgc aggaatcggg ggtctctaaa attgtatctg taaaattaaa
     7561 agaagctcaa gagatgacca attaaaaaac agaagggagt tccttctgtt tttttggatg
     7621 ttcggccata agttaggaga tttcatgatt aaactaatag caacagaaat cggaataagc
     7681 taattgatac aatagagtac aaagaaagcc ttggaaatag cagtttccaa ggctttttag
     7741 ctgtcgatag gtaaaagaga aaagttgtca gagtagatga ttttttattg agacaccctt
     7801 gattggtatt tcgttaaaag ataaagaatg atttcaatta atctttgtta actaatttat
     7861 caaaatggt
//
LOCUS       DAUELZ010000075         7805 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.076, whole genome shotgun sequence.
ACCESSION   DAUELZ010000075 DAUELZ010000000
VERSION     DAUELZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.076"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..689
                     /locus_tag="VHI09_001283"
     CDS             <1..689
                     /locus_tag="VHI09_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEQ6121159.1"
                     /translation="VLSKFTYFLLVCLPLFIPNEKFGKNTKLVILKKLGGLLLIFLFA
                     AMWFRLYGQVKTPYVADFLKEVNVSQQVKNMLESPIVYSSIIIRHMVINLINMNNIFQ
                     FGALSYGITNLFPLYVCFFFFVYISNASKITINIVEKMGIIFVISAIIGATVLAMYLT
                     WTPVGSSTVLGVQSRYLIGIIPLVLLLFSSQQQKFKQIEDILSDKLAIHISLLFILAM
                     LMSTIFRYYH"
     gene            783..2006
                     /gene="pepT"
                     /locus_tag="VHI09_001284"
     CDS             783..2006
                     /gene="pepT"
                     /locus_tag="VHI09_001284"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEQ6121160.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALQLAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELTIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            2047..2535
                     /locus_tag="VHI09_001285"
     CDS             2047..2535
                     /locus_tag="VHI09_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEQ6121161.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHVEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(2522..2719)
                     /locus_tag="VHI09_001286"
     CDS             complement(2522..2719)
                     /locus_tag="VHI09_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEQ6121162.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            2768..3244
                     /locus_tag="VHI09_001287"
     CDS             2768..3244
                     /locus_tag="VHI09_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ6121163.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKTSSSSTTTDTTPSSEEIL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            3259..3939
                     /locus_tag="VHI09_001288"
     CDS             3259..3939
                     /locus_tag="VHI09_001288"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEQ6121164.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPISFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWV
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKSAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    3963..4086
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            4101..4631
                     /gene="infC"
                     /locus_tag="VHI09_001289"
     CDS             4101..4631
                     /gene="infC"
                     /locus_tag="VHI09_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEQ6121165.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDNK"
     gene            4673..4870
                     /gene="rpmI"
                     /locus_tag="VHI09_001290"
     CDS             4673..4870
                     /gene="rpmI"
                     /locus_tag="VHI09_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEQ6121166.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            4929..5288
                     /gene="rplT"
                     /locus_tag="VHI09_001291"
     CDS             4929..5288
                     /gene="rplT"
                     /locus_tag="VHI09_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEQ6121167.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(5579..7789)
                     /locus_tag="VHI09_001292"
     CDS             complement(5579..7789)
                     /locus_tag="VHI09_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEQ6121168.1"
                     /translation="MTSLGKNDKEVATVKKFKTLITGFINTRLGFIITLLFCYWIKTL
                     WAYHTDFSLDLGNIYQVFLTIINPIPLAFLLLGVALYVKNTRAFYICSWVVYIILNIL
                     LISNSIYYREFSDFITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSIT
                     KKIKNDQRPFNKRAAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPA
                     FTLYSGNQTYQAQKERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLES
                     FQQFLIDYKLKEGDKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSL
                     FGLNSGSFMVNYGGENTQFATPSILAQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYF
                     FDSSYFSKQNSKNSFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGE
                     SSEEGFPLAKTDDETINGYFATANYLDAALKSFFDYLKATGLYDNSIFVLYGDHYGIS
                     NSRNSSLAPLLGKDSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLL
                     HILGIDTSQFVQLGQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNP
                     DEMTIAKTKEIRSAVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKD
                     ISAKLGSESTSLYSKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNTKNRE"
BASE COUNT         2544 a         1276 c         1528 g         2457 t
ORIGIN      
        1 tagttctttc aaaattcaca tactttttat tagtatgcct tccattattt attcctaatg
       61 aaaaattcgg aaaaaatacc aagttagtca tcttgaaaaa attgggagga ctattactca
      121 tttttctttt tgctgcaatg tggtttagac tttatggaca ggttaaaacc ccatatgtcg
      181 cggattttct aaaagaagta aacgttagcc agcaagttaa aaatatgcta gaatccccta
      241 tagtctatag cagtattatt attagacata tggtaattaa tctgattaat atgaataata
      301 tttttcagtt tggcgcatta agctatggta taacgaacct tttcccgctt tatgtgtgtt
      361 tcttcttttt tgtttatatt agtaatgcta gtaaaataac tattaatatt gtagaaaaaa
      421 tgggtataat ctttgttatt tcagcgatta taggtgcaac tgtattagct atgtacctga
      481 cttggacacc agtagggtcc tcaactgtct taggtgttca aagtcgttat ctgataggaa
      541 ttatcccatt agtgttgctt ctttttagta gtcaacagca aaagttcaaa caaatagagg
      601 atatcctttc tgacaaactg gctattcata tttcgctttt gtttattctc gcaatgttaa
      661 tgagtactat ttttagatat tatcattaaa ctcttaatta gaggctttaa aaaacttagc
      721 ttgaataagt attttaaagc cttttatgct ataattaaat tgagaaatta ttgaggtggc
      781 ctatgaagta cgataacttg ttagatcgat ttataaaata tgtcaaagtg aatacaagaa
      841 gcgatccaga cagtgagact actcctagta cggagagtca agaagcattt gccttaacga
      901 ttttaaagcc tgaaatggag gcaattggtt tgcaagatgt tcattataat ccagttaatg
      961 gctatcttat tggaactctg cctgccaata atccaacctt gacgcgtaaa attggtttta
     1021 tagcccatat ggatacggct gatttcaatg ctgaaaatgt taatccgcag attattgaca
     1081 attatcaagg aggggacatt acacttggca gttctaatta taagttggat ccaaaagcat
     1141 ttccgaatct taataattat atcggtcaaa cattgattac cactgatggg actacacttt
     1201 taggggcaga tgataaatct ggtattgctg aaatcatgac agcaatagaa ttcttaacat
     1261 cacaaccaca aatagagcac tgtgacatta aagtggcttt tggccctgat gaagagattg
     1321 gggtcggagc tgataagttt gaggtggctg attttgaggt ggattttgct tatactatgg
     1381 atggcggtcc tttaggcgaa ttgcaatatg aaactttcag cgctgcagct ctagaagtga
     1441 cttttttagg cagaaatgtt catcctggta cggctaagga tcaaatgatt aatgcccttc
     1501 agttagcaat agattttcat gagaagctac ctgctaaaga gcggcctgaa tacacagatg
     1561 gttatcaagg cttctatcac ttaacgggac taactggaac agttgaagag gcacgggcat
     1621 cttacattat tcgtgatttt gaagaagcat catttgaagc tagaaaagtg aaagttgaaa
     1681 atatagctca atcgatgaat gctcagctag gaacaaaacg tgtcttggta gagcttaatg
     1741 accaatacta caatatgaaa aaagttatcg aaaaagatat gaccgctatt gagttagcta
     1801 aagaagtaat ggaagagctt actatcaagc ctgtaatcga acccatacgt ggaggaacag
     1861 atggctctaa aatttcattt atgggtattc ccactcctaa tatttttgca gggggtgaga
     1921 atatgcacgg gcgttttgag tttgttagct tacaaacgat ggagagagct gtcgatgtca
     1981 ttattggact tgtttgcaaa gcctaattat catgtaattg atatttagtg gaaggggctt
     2041 ataaaaatga ttaaactatt tggtaaaata aggtatcact ggcagccaga gttgtcttgg
     2101 tctattattt attggtctat tgcgtttgca cctatttttg taggtttatc tttgttgtat
     2161 gagcgcactg aaatacctag tcgggttttt attctatttg ctatttttgc tgtcctagtt
     2221 ggcattggat tacatcgtta tttcattatt gaaaataatg gtattttaag aatcgtatct
     2281 tttaaattgt ttggtccacg taagctactt atttccacta ttactaagat tgaggtgacc
     2341 aaatcaacac tttgtctgca cgttgaagat aaaagttatc tcttttatat gcgcaaatgg
     2401 cctaaaaaat attttcttga tgccttagcg gttaatccat acttccaagg agaagttata
     2461 ttaagtgata actttattaa attagactat tttgaagttt atcagcatga taaaaaagcc
     2521 cttactcgag ggtaagagct ttagttgggc aagatttgac agctaaaata gcgtccttat
     2581 ctgaaggaca aattgactga cttgtttcag atgagcttga aaatgtgacg ataccattgt
     2641 catgataatc aaaaagtgat gaataggttt ggcatagacc acaagcgatg catttttctg
     2701 gaatgataga tactttcata actattattc taatatgatt ttgttaaatt aacaaggggg
     2761 gacgatcatg gctaaagaac catgggaaga aaaaattgtt gatgatacta tagggacacg
     2821 aacacgtaaa tcaagaaatg ctttcattag cacgccttgg ttgactgctt tattaagtgt
     2881 attctttgtc atcattgttg ctatactttt tattttcttc tatacatcaa atagcggtag
     2941 taatagacaa gctgaaacaa atggttttta tggagcatcc actcataaaa aaacaaggaa
     3001 agcttctaac gctaaaaaaa catcaagtag ttcaacaact acagacacaa caccttctag
     3061 cgaagaaata cttgcttcta gtgaaggaac cggcgaaacc cttactgtat tggcaggtga
     3121 gggggcagct tctattgcag ctcgtgcagg tatttctgtg gagcagttac aagcacttaa
     3181 tccagagcac atgactcaag gatattggta tgccaatcca ggagatcaag tcactattaa
     3241 ataattgaag aggaaaaaat gaaagcaatt aaaattgcga ttgatgggcc agcttcaagt
     3301 ggaaaaagta cagtagccaa gattattgca aaaaatcttg gctatactta tctagatact
     3361 ggtgctatgt atcgctcagc aacttatatc gcactgacac atggctatac gggtaaagaa
     3421 gtagccctta ttttagagga acttgagaaa aatcctattt cctttaaaaa agcaaaagat
     3481 ggctcccaat tagtttttct tggggatgag gacgtgacct tagccattag gcaaaatgat
     3541 gtgactaaca atgtttcttg ggtctcagct ttgccagaaa ttagagaaga gctggttcat
     3601 caacaaagac gcattgcaca agctggtggc attatcatgg atggtcgtga cattggaacg
     3661 gttgttctcc cagatgcgga actaaaaatc tttttagtgg cttctgtcga agagcgggca
     3721 gagcgccgct ataaagaaaa cctagaaaaa ggaattgaat cagattttga gacgctaaaa
     3781 gaagagattg cagctcgtga ttacaaggat agtcatcgta aggtttcacc tctaaaatca
     3841 gctgaagatg cccttatttt tgataccaca ggtgtttcta ttgatggcgt ggtgcaattt
     3901 atccaagaaa aagcagaaaa aatagttgac atgtcctaga gcctatgtta gaatagtaac
     3961 aatgagaaaa gcagaagtga gagcttctcg ccttgcgact taggttgtct ggccctaaca
     4021 tgtcaaattc ctatggaata ttatatgtgc gggcttgatt tatcaagtcc gctttcgttt
     4081 ttctctaaaa aaataaagag gtgaagatca tagctaaaaa ggatctattc attaatgatg
     4141 aaattcgcgt tcgcgaagtt cgtctcgttg gtcttgaagg tgaacaatta ggtattaaac
     4201 cattgtctga agcgcaatca ttggctgatg cttcaaatgt tgatttggtt ttaatccagc
     4261 cacaagctgt tcctcctgtt gccaaactta tggactatgg aaagttcaaa tttgagtatc
     4321 aaaagaaaca gaaagaacaa cgtaaaaaac aaagcgttgt gactgtgaaa gaagttcgtc
     4381 tcagtccagt tatcgataaa ggtgactttg aaacaaaact tcgtaacggt cgtaaattcc
     4441 ttgaaaaagg aaataaagtt aaggtttcta ttcgtttcaa aggacgtatg attactcata
     4501 aagagattgg tgccaaggta ctagctgact ttgctgaagc gactcaagat attgctatca
     4561 ttgagcaacg agcaaaaatg gatggacgcc aaatgtttat gcaacttgca ccaatttcag
     4621 acaataagta attgtcacag tttcattata tctttaagag gagaatacta aaatgccaaa
     4681 acaaaaaaca caccgcgcat cagctaaacg ttttaaacgt acaggttcag gcggtttgaa
     4741 acgcttccgc gcctttacat cacaccgttt ccacggaaaa actaaaaaac aacgtcgtca
     4801 tcttcgtaaa gctggtttgg ttagctcagg agatttcaaa cgtatcaaag caatggttac
     4861 tggtctttaa ttttaaaggc agtagacatt tacgagtatt ataagaatta tttgaaggag
     4921 aactattaat ggcacgtgtt aaaggtggcg ttgtttcacg caaacgtcgt aaacgtattt
     4981 taaaattagc taaaggttac tatggagcaa aacacatctt gttccgtact gcaaaagagc
     5041 aagtgatgaa ctcttactac tatgcatacc gtgatcgccg tcagaaaaaa cgtgatttcc
     5101 gtaaactatg gatcactcgt atcaatgcgg ctgctcgtat gaacggtttg tcatactcac
     5161 aattgatgca cggtttgaaa cttgctgaaa tcgaagtaaa ccgtaaaatg cttgctgatt
     5221 tagcagtagc tgatgcagca gctttcacag ctcttgctga tgcagccaaa gcaaaacttg
     5281 gcaaataaga aacgctatac ctaaaaaata actttctatt acctatagaa agttataaag
     5341 aaacaaaaat gaaggagacg atggacgtct tctttttatt atactcaata taataaaaag
     5401 aagtttccca tgttttatta ccagtaatgt gggatattta gatggtagca agaagtttta
     5461 tagttgattt gttttcttta ggtctaatta gcatattttg attactgata aacttgaata
     5521 tcgccaataa aaaagaagca aaattattat tttgcttcta gtcagatcat taaattgatt
     5581 attctctatt tttggtgtta tcctctttta gttcagaagt tgccgcgtca gcttctacgg
     5641 gatttagttc taagtaagat ggtgctttaa aaagtttctg agttgattta tgaccatttt
     5701 tactgtataa acttgttgac tctgatccga gttttgctga aatatctttc agttgtttta
     5761 actgcttagt gtagataaac tggttaggat caatagcttt tagaccattt tgagtatcaa
     5821 agcgcaggag atcacccgtt tgaatagcgt cgctagctgc tagttgttga gcaacagcag
     5881 agcgaatttc cttagttttg gctatagtca tttcatcggg attagtgatt tctaaacctg
     5941 tttgcgtgtt gtacagacgt ccactatagt tagtgtattc aggagtcata taagtcccag
     6001 atgttcgttg tgccacaatc tggctatttt gaggtgataa taaatcttgt cctaattgaa
     6061 caaactgact agtgtcaata ccaagtatgt ggagtaaagt aggaagagca tcgatttcac
     6121 cgccaaaggt ttctttgata ctaccattcg tataacctgg aatatggatc atatacggga
     6181 ctcgttgtag catagcatta tcatattcag accaagtttc agaatcttta ccaagaagtg
     6241 gagcaagact agaattacga gaatttgaaa taccataatg gtcaccatat aaaacaaaaa
     6301 tagaattgtc gtacaaacca gtggctttca agtaatcaaa aaaagattta agggcagcgt
     6361 ctaaataatt ggcagtagca aagtaaccat tgatcgtttc atcgtctgtt ttggctaaag
     6421 gaaaaccttc ttcactactt tcgcctttta gactagtata aggatagtga ttactcacag
     6481 tgataaactt ggtataaaaa ggttgttgca tctgttcaag atattttatg gaatccttaa
     6541 acatgtactt atcatttaac ccatattgaa aagagttctt actattttgt ttagagaagt
     6601 agctagaatc aaaaaaataa ttatagcccc attgtttata agcattattg cgattccaga
     6661 aagttccaac gttaccatga aagacagcgc tggtatagcc acctttttgg gctaaaatac
     6721 ttggagtagc aaattgtgta ttttcaccac cgtagttcac cataaaagaa ccactattta
     6781 aaccaaataa ggaattctcc atcatggttt cagcatcaga agttttacct gctttaacct
     6841 gatgaaaaaa gttagggaat gctaaggtag catttgagtg ataaagcgag ttgataaaag
     6901 gcgttacctc atattcttta tctccttctt ttagtttata atctattaag aattgttgaa
     6961 agctttctaa atgaagaaca ataatatttt tgccttttcc aattccaaaa tactggggat
     7021 caggtgctgc ataatgtcct ttgacataag ttttaacatc aattaattct tctgcagtag
     7081 ctccatttcg ttctttttga gcttgatagg tttgattgcc gctatagagg gtaaacgctg
     7141 gtaatcctaa agctctcaca atataagtat ttgaaaagcc acgtgttaat agttccggac
     7201 gatcaatttc tgctaaaaaa agattaatag aaagcaatag actagataaa gcggtgatgg
     7261 caaatgctgc gcgcttgtta aaaggacgtt gatcattttt gattttttta gtgatagata
     7321 aactaattag aataataaaa tcaagaatat agataatatc ccagatgcgt aaaaggttta
     7381 aagcagagtc ccctaatcca gcggagactt tactgctagc tagcatggca ctcactgtaa
     7441 taaagtctga aaattctcgg taatatatag aatttgatat caagagaatg ttaaggataa
     7501 tataaaccac ccaagaacag atataaaagg ctcgggtatt cttaacgtaa agagcgaccc
     7561 ccaaaagtaa aaaagctagt gggattggat tgataattgt tagaaagact tggtatatat
     7621 ttcccaaatc tagtgaaaag tctgtatgat atgcccacag agtctttatc cagtaacaaa
     7681 agagtagagt aattataaaa cccaatcttg tgttgataaa acctgtgata agtgttttaa
     7741 atttcttcac agtagcaact tccttatcgt tttttcctaa agatgtcatc tccaaataaa
     7801 atctc
//
LOCUS       DAUELZ010000076         7787 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.077, whole genome shotgun sequence.
ACCESSION   DAUELZ010000076 DAUELZ010000000
VERSION     DAUELZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.077"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..585
                     /locus_tag="VHI09_001293"
     CDS             <1..585
                     /locus_tag="VHI09_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ6121169.1"
                     /translation="SYLIEGMTAKEALKTKSFYCLWVILFINITCGLGLISVVAPMAQ
                     DLTGMSPEMSAIVVGAMGIFNGFGRLVWASLSDYIGRRVTVILLFLVSIIMTISLIFA
                     HSSLIFMISIATLMTCYGAGFSLIPPYLSDLFGAKELATLHGYILTAWAIAALTGPML
                     LSITVEWTHNYLLTLCVFIVLYILGLMVALRLKK"
     gene            651..1358
                     /locus_tag="VHI09_001294"
     CDS             651..1358
                     /locus_tag="VHI09_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEQ6121170.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQTTGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLRVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLIHPNLTTSLVPLS
                     DGLLMIRKNQADIVLPD"
     gene            1421..2476
                     /gene="prsA"
                     /locus_tag="VHI09_001295"
     CDS             1421..2476
                     /gene="prsA"
                     /locus_tag="VHI09_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ6121171.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            2863..5481
                     /gene="alaS"
                     /locus_tag="VHI09_001296"
     CDS             2863..5481
                     /gene="alaS"
                     /locus_tag="VHI09_001296"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEQ6121172.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYNPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVLTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAETV"
     gene            5841..6056
                     /locus_tag="VHI09_001297"
     CDS             5841..6056
                     /locus_tag="VHI09_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6121173.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            6053..6814
                     /locus_tag="VHI09_001298"
     CDS             6053..6814
                     /locus_tag="VHI09_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEQ6121174.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEEDDISENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVRSATMTRQFYR"
     gene            6833..7528
                     /locus_tag="VHI09_001299"
     CDS             6833..7528
                     /locus_tag="VHI09_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ6121175.1"
                     /translation="MKGFINYLKIAVFIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIVGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
     gene            complement(7625..>7787)
                     /locus_tag="VHI09_001300"
     CDS             complement(7625..>7787)
                     /locus_tag="VHI09_001300"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ6121176.1"
                     /translation="TLKRELVNDAHFATIEQAQLEIFKYSETYYNPKRLHSALGYLSP
                     VEFEKIVTH"
BASE COUNT         2461 a         1335 c         1622 g         2369 t
ORIGIN      
        1 agttatttaa tcgaaggaat gaccgctaaa gaagcactta aaacaaaatc attttattgt
       61 ctttgggtaa ttttatttat caatatcaca tgtggcttag gacttatctc agttgttgcg
      121 ccaatggcac aagatctaac tggtatgagt ccagaaatgt cagcaattgt ggttggtgct
      181 atggggattt ttaacggttt cggtcgttta gtgtgggcta gtttgtcaga ctatattgga
      241 cgccgtgtga cagtaatact tttattttta gtaagcataa ttatgactat tagtttaata
      301 tttgcacata gttccctaat tttcatgata agcattgcca cccttatgac ttgttatggt
      361 gcgggttttt ctctcattcc gccttattta agtgatcttt ttggggcaaa ggaactggct
      421 acattacatg ggtatatatt gacagcttgg gcaatagctg cattaactgg acctatgtta
      481 ttatctatca ctgttgaatg gactcataat tatcttctca ccttatgtgt ttttattgtc
      541 ttatacattt taggattaat ggtagcatta aggttaaaga aatgatgtcc tataaaaaat
      601 ttaaaagtaa accctagcaa agcctagggt ttttttatat aattaggttt atggttaaat
      661 catacagtaa aacagcaaat cataatatgc gtcgtcctgt tgtcaaagaa gagcttgttc
      721 attatatgcg aacacggcag aagcaaacca caggtttttt agcagagtta gagcaattcg
      781 caagacaaga aaacattcct attattcagc ctgaggtcgt ggcttatttc aggtttttat
      841 tgcaaagctt acagccaaaa cacattttgg agattggcac agccattggt ttttctgcgc
      901 ttttaatggc tgaaaatgca ccagatgcga cgattgtgac tattgatcgt aatcgagaga
      961 tgatcgattt tgctaaagca aattttgcta agtacgacag tcgccaacag atccgcctgt
     1021 tagaaggaga tgcagcagat atattatcta cacttgaagg aaattttgac tttgtgttta
     1081 tggattccgc caaatccaaa tatattgtct ttctaccaga gattttaaga ctgttaagag
     1141 tcggtggtgt agtcattttg gatgatgtct ttcaaggagg tgacattaca aaaccgatcg
     1201 aagacattcg acgaggtcag cgtactattt atcgtggctt acagtctttg tttgatgcca
     1261 ccttaattca tccaaaccta acaactagcc tggtccctct tagtgatgga ttattaatga
     1321 ttcgtaaaaa tcaagcagat attgtattac cagattaagt aacattgata aatttatgtt
     1381 ataataaatt agttaaagat aaaaaggagc tcagaagtaa atgaaaaact caaataaact
     1441 cattgctagt gttgtgacat tggcctcagt gatggcttta gcagcttgtc aatcaactaa
     1501 tgacaatact aaggttattt cgatgaaagg tgatacaatt agcgttagtg atttttacaa
     1561 tgaaacaaaa aacacagaag tatcgcaaaa agcgatgcta aatctggtaa ttagtcgtgt
     1621 ttttgaagct caatatggtg ataaggtttc aaaaaaagaa gttgaaaagg cgtatcataa
     1681 aacagctgaa cagtatggcg cttcattctc tgctgctttg gcacaatcaa gcttgacacc
     1741 tgagactttt aagcgtcaga tccgctcttc aaaattagta gaatatgcgg ttaaagaagc
     1801 agctaaaaaa gaattgacaa cacaagaata taagaaagca tatgaatctt atactccaac
     1861 aatggcagtc gaaatgatta ctttagataa tgaagagaca gctaaatcag tcttagagga
     1921 actaaaagcc gaaggcgcag actttacagc tattgctaaa gaaaaaacaa caacacctga
     1981 gaaaaaagtg acctataaat ttgattcagg cgcgacaaat gtaccgactg atgtcgtaaa
     2041 agcggcttca agtttgaatg agggtggcat atcagacgtt atctcggttt tagatccaac
     2101 ttcttatcaa aagaagtttt acattgttaa ggtgactaaa aaagcagaaa aaaaatcaga
     2161 ttggcaagaa tataagaaac gtttgaaagc tatcattata gctgaaaaat caaaagatat
     2221 gaatttccaa aacaaggtta ttgcaaatgc attggataaa gctaatgtaa aaattaaaga
     2281 caaagctttt gctaatattt tggcgcaata tgcaaatctt ggtcaaaaaa ctaaagctgc
     2341 aagtgaaagt tcaacaacca gcgaatcatc aaaagctgca gaagagaacc catcagaatc
     2401 agagcaaaca cagacatcat cagctgaaga accaaccgag actgaggctc agacgcaaga
     2461 gccagctgca caataagtag gattttattg actaatcagt taaaaaaact tataataatt
     2521 tagttaagaa ttgtttatca aggattagat acagaaaagt gacggttgct gcgagtcatt
     2581 gataattaga taaacatgct acttaacttt acaacaattg atatcgtaaa ttaagaatga
     2641 caagttcatt gaatagaggt ggtaccgcgg ttttcgccct ctgtgagatg gacttgtttt
     2701 gtattgagga ctatttgaaa tcaacgaaag gaatagagta gactaggctg ccaggcttat
     2761 agctgatctc ctagcaagaa agacgagctg tgtcaactgg tgtgattaca ttactagctt
     2821 cagcataaca ctgtttttct tactctttgt atctaaattt ttatgaaaga attatcgtct
     2881 gcacaaatcc gccaaatgtg gttggatttc tggaaatcta aaggacattg cgttgagcct
     2941 tcagctaact tggttcctgt gaatgaccca acgcttcttt ggatcaactc aggtgttgca
     3001 accttgaaaa aatactttga tggttcagtg attccagaaa atccacgtat taccaatgca
     3061 caaaaatcaa ttcgtactaa tgatattgaa aatgttggta aaacagcacg tcaccatact
     3121 atgtttgaaa tgcttggtaa cttctcaatt ggagactatt tccgtgatga agctattgag
     3181 tggggatttg aactcttgac aagtccagac tggtttgatt tccctaaaga taagctctac
     3241 atgacttatt acccagatga caaggattca tataaccgtt ggattgcttg tggcgttgaa
     3301 ccaagtcact tggtgccgat cgaggataac ttctgggaaa tcggtgctgg tccttcaggt
     3361 ccagatacgg agattttctt cgatcgtggt gaagatttcg atccagaaaa tatcggactt
     3421 cgcctcttgg ctgaagatat cgaaaacgat cgttacatcg aaatctggaa catcgttctc
     3481 tcacaattca atgctgaccc agccgtacca cgttcagaat acaaagaatt accaaacaaa
     3541 aacattgata caggtgctgg tcttgaacgt cttgcagctg ttatgcaagg ggcaaaaaca
     3601 aactttgaaa ctgacctctt catgccaatc atccgtgaag tagagaagtt gtcaggtaaa
     3661 acttataatc cagatggcga caacatgagt ttcaaggtta tcgctgacca catccgtgcg
     3721 ctttcatttg ctatcggtga tggtgcgctt cctggaaatg aaggtcgtgg ttacgttctt
     3781 cgtcgtcttc tccgtcgtgc tgttatgcac ggtcgtcgtc ttggcatcaa cgaaactttc
     3841 ctttacaaat tggttctaac tgttggacaa atcatggaaa gctactaccc agaagtgctt
     3901 gaaaaacgtg attttatcga gaaaatcgtt aaacgtgagg aagaaacatt tgctcgtact
     3961 atcgatgcag gtagcggtca cttagattca ttgcttgcgc agcttaaggc tgaaggtaag
     4021 gatactcttg aaggtaaaga tatcttcaaa ctttatgata cttatggatt cccggttgaa
     4081 ttgacagagg aattggcaga agatgcaggc tacaagattg atcacgaagg ctttaagtca
     4141 gccatgaaag aacaacaaga ccgtgcgcgt gcagctgttg ttaagggtgg ttcaatgggg
     4201 atgcaaaatg aaaccctagc tggtattgtt gaagaatcac gattcgaata cgacacatat
     4261 agtcttgaat caagtctttc agtcatcatc gctgataatg aacgtaccga agctgtttca
     4321 gaaggtcaag ctcttcttgt ctttgctcaa acaccattct atgctgaaat gggtggacag
     4381 gttgctgaca caggtagaat caaaaatgat aagggtgaca cagttgctga ggttgttgat
     4441 gttcaaaaag caccaaatgg tcaacctcta cacactgtaa acgttttggc atcactttca
     4501 gttggaacaa actacacact tgaaatcaac aaagagcgtc gtttggctgt tgagaaaaac
     4561 cacacagcaa ctcacttgct ccatgcagcc cttcacaatg ttatcggtga acacgcaact
     4621 caggctggtt cattgaacga agaagaattc ttgcgctttg attttactca ctttgaagca
     4681 gtaagcaatg aggaacttcg tcacattgaa caagaagtta atgagcaaat ttggaacgct
     4741 cttacaatca caacgactga aactgacgtt gaaaccgcaa aagagatggg agcaatggcg
     4801 ctttttggtg agaaatatgg taaagtggtt cgtgtggttc aaattggtaa ttattctgtt
     4861 gaactttgtg gtggaactca cttaaataat tcttcagaaa tcggtctctt caagattgtc
     4921 aaagaagaag gtattggttc aggtactcgt cgtattattg cagttactgg tagacaagct
     4981 tttgaagctt atcgtaacca agaggatgcc ctaaaagaga tcgctgctac tgtaaaagct
     5041 ccgcaattga aagatgcagc agctaaagta caagctctta gcgactcact tcgtgatctt
     5101 caaaaagaaa atgcagagct taaagaaaaa gcagcagctg cagcagctgg tgatgtcttt
     5161 aaagatgttc aagaagctaa gggcgtgcgc ttcattgcta gtcaagttga tgttgcagat
     5221 gcaggggcac ttcgtacatt tgctgataac tggaaacaaa aagactactc tgatgtgctt
     5281 gttctcgtag cagctatcgg tgagaaggtt aatgtccttg ttgcaagtaa aactaaagat
     5341 gtccacgctg gtaacatgat taaagaattg gcaccaattg tagcaggtcg tggtggaggt
     5401 aaaccagaca tggctatggc aggtggtagc gatgcaagta aaattgcaga gctgctagca
     5461 gcagttgctg aaacagtgta aatccataaa aaccaagtta tttagaactt ggttttttat
     5521 atttgaaatg actatcatca taaccttttt gactattatc gttgtcaaaa agaaaaagat
     5581 atgttacaat gatagtaaca aatagacatg aggaggtgtt agagaaaatg acacatggta
     5641 tcaaacttaa gaggcagatt tgcttatggg caagtatcct gactcttttc attttaagtt
     5701 atcatcttat cagggtaatc tggtttggag tacccttgag ctatctggtt ttgggcttta
     5761 caggactagc cttgctgttg aatacttttt cttattttag aaagtaaatt ataagttaaa
     5821 ggagaatgaa ggagatggca atggaggtta ttcttaaaaa tcggctcaaa gaattacgag
     5881 ccagagatgg aattaatcaa acggaaatgg ctaaactggc aggagtatcg aggcagacca
     5941 tcagtctcat tgagcgtaac gagtacaccc catcagttat aattgccatg aaaattgcaa
     6001 aggtatttca ggaaccagtt gaagaggtgt ttcgtctagt ggaggtcgag gaatgaaaaa
     6061 gaagaagaat agttttttac gcttgttaaa aatgttattg ctatcaagtt tggcgggagg
     6121 gattataggt ggcatggttg gagccttttt aggttaccat ggagagagat tagatcagct
     6181 cacgttccta aaagatgatg tgattaacct gattatctta ttaaatcgtc tagtagttgt
     6241 cacaggcctc acactttctt ttgttttttt aacgcaactg aaaaaagaga cggctgttta
     6301 taacacaatt gaagaggatg atatttctga aaatggttac cgtcagctga ataaaaagca
     6361 tgcttatacc atgttattga ttgccgtagc ttctatttta tccatgtgca atgtgctact
     6421 tggtctgacc ttaaccaatg attcacagca tgccatgctg gccattccgc ttctggatat
     6481 ccttttatta ttaatggtca ttccttttca agctttagcc atgaaacgtt acaatgccat
     6541 tcggggaacc gatgttcctt attttccaaa tttgaaagaa ttaaaacata atatcatggc
     6601 tctagatgag gcagagttac aagcctatca taagacatcg tttgaaagtg tgttgtcatt
     6661 aaatggagtc atcatccctt ccttgtatgt gattttattt tttgtctatc tatttactgg
     6721 tcaagtggag ttaacagcca tcttagtttt ggtgttaatt cagctctatt tactcgttag
     6781 gagtgcaaca atgaccagac aattttaccg ttagcctaaa aggagactgt ttatgaaagg
     6841 attcattaat tatttaaaaa tagcagtgtt cattattctg gctatggttt tcaatgtcct
     6901 tccgatgatt ttattgcaaa agcaacacga tattcctatg gtgcttaatt ggggaattgg
     6961 tattttctac ttggttattg ttggaagtgt ccttattgta ttatggcgtc tttatcaagc
     7021 taagcaagac acccctatta aacagcaaaa aatgagattg gttgactggg gttatttagc
     7081 attattttgg ttaatcatcc gtgtgatagc tattgtaggt acccttgtta accagctatg
     7141 gtctggtcaa caagtgagtg ctaatgatgc tgcaatacat accttagcta gacttatcaa
     7201 aggtggtttc ccgctttata ctgccctatt tgtacttgtg atagctttta tcgctcctat
     7261 tatggaagaa ctagtcttta gaggatttcc tatgattgat ctcttcaaag gaaaatcact
     7321 taaggtggca ggtttagtga cctctcttgt ttttgcttta ccacatgcca ccaatagtgt
     7381 tgaatttatc atgtacagct gtatgggcat ttttctcttt gttgcctatc aaagacgagg
     7441 aaacttaaaa gacgctatct tgttacatat ttttaataac ttgattgaag tcattttgtt
     7501 aatgtcaata ggcttaggag tcatataata aaaagcttcg aatgactgaa ctgcactcca
     7561 aaagttagac aaaaaatcta acatttggtt ggacttgtca agaaaaaata gacataaagt
     7621 taagctaatg agtaactatt ttttcaaatt ctacaggtga gagatatcct aaagctgaat
     7681 gcagacgttt agggttatag taagtctcac tatatttaaa aatctcaagc tgagcttgct
     7741 caatagttgc gaaatgagca tcattgacaa gctctctttt taatgtt
//
LOCUS       DAUELZ010000077         7707 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.078, whole genome shotgun sequence.
ACCESSION   DAUELZ010000077 DAUELZ010000000
VERSION     DAUELZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.078"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            549..938
                     /locus_tag="VHI09_001301"
     CDS             549..938
                     /locus_tag="VHI09_001301"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEQ6121177.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
     gene            1032..2084
                     /locus_tag="VHI09_001302"
     CDS             1032..2084
                     /locus_tag="VHI09_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ6121178.1"
                     /translation="MNKKFISLGLASVAVLSLAACGNRGASKGGAAGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            2223..3755
                     /locus_tag="VHI09_001303"
     CDS             2223..3755
                     /locus_tag="VHI09_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121179.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIIKNLVKEGKSIILITHKLDEIRAVADRVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGIPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNNEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVLPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            3748..4812
                     /locus_tag="VHI09_001304"
     CDS             3748..4812
                     /locus_tag="VHI09_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121180.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            4814..5770
                     /locus_tag="VHI09_001305"
     CDS             4814..5770
                     /locus_tag="VHI09_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121181.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(5983..7701)
                     /locus_tag="VHI09_001306"
     CDS             complement(5983..7701)
                     /locus_tag="VHI09_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ6121182.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKSKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFETLRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSAGLIQVIGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMNKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
BASE COUNT         2304 a         1278 c         1596 g         2529 t
ORIGIN      
        1 tcgagtaacc ttttttaatg tattttaact gaaaaaggct ttcataattt taaaaaaatg
       61 ttaaaatagg tgtataacaa ctgattacca agagattatt atcattatct tctaaggtgt
      121 cattgttatg gtgacaatca accatacgct actaaagata ggtagcgttc gatcacaggt
      181 tgatcaagtg attgaaaaat caatatttat aattttacaa atatctgtat caatccatgt
      241 tgaatagtct atgcttctaa ggtgtcgacg ttaatgtact gacgggaccg ttatagtctt
      301 tctttttgga gaaacgtcgg atgccagtga actagttgaa gttaaagatg ttaattcaaa
      361 cagtgctgaa gagattgata tcgttttgaa cggtcgttta cttcaaagca aagaaattac
      421 cagtctatcg aagatgatat aagtgctaaa gttagatcac gttaattatc tatcaagtcg
      481 ggtgcatatt tcgttaaagt atctacaggg gtttcaatag atgtagctat tatgaaaggt
      541 gatgttaaat ggggactact gatttagtta gttgtgctgt acaagctagt gagtatgctt
      601 atgtccctta ctctcatttt ccagtaggag cagctttaaa aacaaaagat ggaacgatct
      661 atactggctg taatattgaa aatgttagtt ttggtctgac aaattgtgga gagcgaacag
      721 ctatatttaa agccatatct gatggtcata aagaattggt tgaaattgct atttatggag
      781 aaactatgca acctgtatct ccttgtgggg cttgtcgtca ggtaatggca gaattttttg
      841 ctccttcttc tttagtcact ttaattgcta aaaatggtca aactgttgaa atgactgttg
      901 gagatttact cccctattct tttacagatt taatataatc tgctttaatt tctgttatct
      961 agccgtcttg gctttatatt tactttcagc aacttatttg ctagaaatct tttttaggag
     1021 ggttcatatt catgaacaag aaatttatta gtcttggttt agcgtcagtg gctgtgctga
     1081 gtttagctgc ttgtggtaat cgtggtgctt ctaaaggtgg ggcagcagga aaaactgatt
     1141 taaaagttgc aatggttacc gatactggtg gtgtagatga taaatcattc aaccaatcag
     1201 catgggaagg tctgcaatct tggggtaaag aaatgggcct tcaaaaagga acaggtttcg
     1261 attattttca atctacaagt gaatctgagt atgcaactaa tctcgataca gcagtttcag
     1321 gagggtatca actgatttat ggtatcggct ttgcattgaa agatgctatt gctaaagcag
     1381 ctggagataa tgaaggagtt aagtttgtta ttatcgatga tattatcgaa ggaaaagata
     1441 atgtagccag tgttaccttt gccgaccatg aagctgctta tcttgcagga attgcagctg
     1501 caaaaacaac aaaaacaaaa acagttggtt tcgtgggcgg tatggaagga actgtcataa
     1561 ctcgatttga aaaaggtttt gaagcaggag ttaagtctgt tgacgataca atccaagtta
     1621 aagttgatta tgctggatca tttggtgacg ctgcaaaagg aaaaacaatc gcagcagctc
     1681 agtatgcagc aggtgctgat gttatttacc aggcagcagg aggcactgga gcaggtgtat
     1741 ttaatgaagc aaaagctatt aatgaaaaac gtagtgaagc tgataaagtt tgggttattg
     1801 gtgttgaccg tgatcaaaaa gacgaaggaa aatacacttc taaagatggc aaagaagcaa
     1861 actttgtact tgcatcatca atcaaagaag ttggtaaagc tgttcagtta atcaacaagc
     1921 aagtagcaga taaaaaattc cctggaggaa aaacaactgt ctatggtcta aaagatggcg
     1981 gtgttgaaat cgcaactaca aatgtttcaa aagaagctgt taaagctatt aaagaagcga
     2041 aagcaaaaat taaatctggt gacattaaag ttcctgaaaa atagtgttta agaacaagaa
     2101 gcgcgacctt tgtcggtcgc tctttttaaa ctgagataat ttttatctca gtaaagtttg
     2161 tgataggtga tagatcatag gctttactga aataaaagat tgcattgaaa ggaaatgatc
     2221 ctatgacaca gcatgtcatt gaaatgagag aaatcacgaa aaaatttggt gattttgttg
     2281 caaatgacca cattaactta aatgtcagaa aaggtgagat tcatgcctta ttgggtgaaa
     2341 atggggcagg taaatcaact ttgatgaata tgctagctgg tctcttagag cctactagtg
     2401 gagaaattgt cattaatgac aagccagttc aaattgattc gccatctaaa tcagctaagc
     2461 ttggtatcgg aatggtacat caacatttca tgttagtaga agctttcacg gttgctgaga
     2521 atattatttt aggaaatgaa gtagtgaaaa atgggtgttt agatcttaac caagcatcga
     2581 aagatattaa ggtactatcc gaaaaatatg gccttgcaat caatccatca gctaaagttt
     2641 ctgatatttc agtaggagca caacaacgtg ttgagatttt gaaaaccttg tatcgtggtg
     2701 cagatattct tatctttgat gagccaactg ccgttttaac acctgcagaa attaaagaat
     2761 tgatgactat cattaagaat ctcgtgaaag aaggaaaatc aattatttta attacccata
     2821 aactggacga gattcgtgct gtcgccgacc gtgtcacagt tattcgtcgt ggtaagagta
     2881 tcgaaacagt tgacgtggca ggagcaactt cacaagattt agctgagatg atggtgggac
     2941 gctcggtttc ctttacaact agcaaaaaag ctgctgaacc aaaagatgtg gtcttatcca
     3001 tcaaaaatct tgaagtagat gaaaatagag ggattccagc ggttaagggg ctatctctag
     3061 atgtccgagc tggtgaaatt gtagggattg ctggaattga cggaaatggt caatctgagt
     3121 taattcaagc tattacaggt ttacgtaaag tcaaatctgg aagtattatg attaaaaaca
     3181 atgaagtcac tcatttgtct tcacgcaaaa ttactgagtt atcagtgggt cacgtgccag
     3241 aagatcgtca tcgagatggt ttgatactag atctttcttt agctgaaaat acagccttac
     3301 aaacctatta caaacaacct ctaagtcaaa atggaatctt aaattatact aaaattaatg
     3361 actatgctcg tcaattaatg aaagaatttg atgttcgcgg agccaatgag ctagtaccag
     3421 ctagaggatt ttctggtggt aaccaacaaa aggccattat tgctcgtgaa gtggaccgtg
     3481 atccagattt acttattgtt agccaaccaa cacgtggtct tgacgtagga gctattgaat
     3541 atatccataa gcgtttgatt aaagaacgtg ataaaggtaa agctgtcctc gttgttagtt
     3601 ttgaacttga tgaaatattg aatttatctg atcgaattgc tgttattcat gatggtaaaa
     3661 ttcaaggtat cgtattgcca gaaaatacca ataaacaaga attgggtatt ttaatggcag
     3721 gtggttcaat tcataaggag gaaggtcatg tctaaaaatg cgcaaaaaat tgctgtacca
     3781 ttgatttctg tcttgctagg atttttactt ggcgctatta ttatggttat ctttggttat
     3841 gatcctatct gggggtatga aggtttattc caaattgcct ttggtagtgt taaaaatatt
     3901 ggagaaatat tccgttcaat ggggccattg attttaatag cgcttggctt tactgttgct
     3961 agccgtgcag gcttctttaa cgttggtttg tctggacagg ctttagctgg atggatttca
     4021 gcaggttggt ttgcactctt aaatccagat atgcctcgtc cattattgat tcttatgaca
     4081 gctcttatcg gaatgattgc tggtggtatt gctggagcta ttccagggat acttcgtgct
     4141 tatcttggta caagtgaagt tatcgttacg attatgatga actacatcat tttatatgtc
     4201 ggaaatgcta ttgttcaacg tggttatcct gagagtgtca aacaaagtat tgattcaact
     4261 attcaagtta gtgataatgc tagttatcaa acgcattggt tatcagccct aactaataac
     4321 tctcgtatta atattggtat tttctttgcc attattgcca ttgccctaat ctggttcttg
     4381 cttaacaaaa ctactttagg ttttgagatt cgttcagttg gtctcaaccc acatgcttca
     4441 gaatacgcag ggatgtcatc aaaacgtact atcattttat caatgattat ctctggagct
     4501 cttgcaggtc ttggaggagt agttgaaggt ctaggaacct ttgaaaatgt ttttgtacaa
     4561 ggatcttctt tagcagttgg ttttgacggt atggcagtta gcttattagc agcaaattca
     4621 ccgcttggaa tatttttctc atcattccta tttggtgtct taaatattgg tgctccagga
     4681 atgaatattg caggcattcc accagaactt gttaaagtag taactgcatc aatcattttc
     4741 tttgttggtt cacattacct cattgaacgt tacattattc gccctaaaaa attagtgaaa
     4801 ggtggtaaat aagatgagtt tagtaacaat ttttgcctta ttggtgtcat ctatgcttat
     4861 ttatgcaaca ccactcattt tcacaagtat tggaggaact ttttcagagc gctcaggcgt
     4921 tgttaatgtt ggtttagaag gtatcatggt catgggagcg ttctctggga ttgttttcaa
     4981 cttagagttt gctgaaacat ttggaaaagc aacgccttgg atagccgttt tagtcggtgg
     5041 tattgttggt ttgatttttt ctttgattca cgctgtagca accatcaact ttcgagctga
     5101 ccacattgtc agtggtacag tgttgaactt gttagcacct tcttttgctg tcttcttggt
     5161 taaagctatg tacggtaagg gacaaacaga caacattcaa caatcttttg ggaagtttga
     5221 tttcccaggt ttatcacaaa ttcctgtgat tggtgatatt ttctttaaaa atactagcct
     5281 tattggttac ttcgccattg ctttttcgtt ctttgcttgg tttttgcttt ataaaaccag
     5341 gtttggtctg cgtttacgat cagttgggga acaccctcag gcagctgaca cacttggtat
     5401 taacgtctat ttgatgaaat actatggtgt gatgatctca ggttttcttg gtggaatagg
     5461 tggagctgtt tatgctcagt caatctccgt taactttgct gtaacaacta tcttaggtcc
     5521 tggatttatt gctttggcag ctatgatctt tggtaaatgg aatccagttg gcgccatgct
     5581 atctagtctt ttctttggct tatcgcaaag tttagccgtt attggtgctc aattaccatt
     5641 actagaaaag attccaacgg tttacttaca gattgcacct tatatggtaa cgattattat
     5701 tttagctgct ttctttggtc aagcagttgc accaaaagct gatggaatca actatattaa
     5761 atctaaataa cgttatatta gtaagcctct tagtgaggtt taacagcact cctaaagcaa
     5821 gatagttagg tctagcttta agggtgcttt tttgggactt accgcaagtt tggattgcca
     5881 gtcaccatca caatagaagc aagaatagac taaaaagaac taagtcagcg gcttagttct
     5941 ttttgcttgt ctttcgtttt tactgatagc tcttaagttg ttttagccaa caaaggtatt
     6001 gatttctgtt tcaatgttag caattttttc ttgagcgatg tcaagagtat cacctattgt
     6061 ggcaatatag aacttgattt ttggttcagt tcctgaaggg cgaactgcaa tccatgaatc
     6121 atcagctaac gtgtacttca aaacattact tggaggagtt gttaagttag atattgttcc
     6181 atcattttta gtagcggttt gtttttggaa atcttctaat aatacaatat cagtattgtt
     6241 aaattgtttt ggaccatttt cacggaattt gttcataatc ttcttaattt cagcagctcc
     6301 atccacacct gaaagtgtta ctgaaatcgt tttttcagca aaatagccat attctttgta
     6361 aatttcatca ataccatctg caagtgttaa accacgcgaa cggtaataag ccgcgatctc
     6421 agcaaccaaa agaactgctt gaatagcgtc tttatcacgg acaaatggtt taataagata
     6481 tccgaagctt tcttcaaaac caaacatata ggtgtggtta tgtttttctt caaactcttg
     6541 aattttctca gcgatgaatt tgaatccagt taaaacgtta aacattgtag cgccataact
     6601 ttcggcaatt tttgttacta attctgttga tacgattgat ttagcgagag cagcattttc
     6661 tggaagtgtt cctgctgttt tatgagcttc caggatgtat ttcgcaataa tcgctccaat
     6721 ttgatttcca gaaagatttt tataagaacc atctggttgg cgaatttcaa cgcctaggcg
     6781 gtctgcatca ggatcggtag ctactaagac atctgcatca acttctcgtc cgagttcttc
     6841 agcaagagca aaggcagctt ggctttcagg gttaggcgat ttaacagtag aaaagtctgg
     6901 atctgcctta gcttgtgatt ctactacaac aacagattca aaaccggctt gagcaagggc
     6961 acggcgtgtc aacatctcac cagttccgtg gagaggagta tagacaattt tcatgtcttt
     7021 tccaaagtta ttgattaaat cttgattgat attaacatct ttaacctctc taagatattc
     7081 catatcaaca tcttcaccga taacttgaat aagacctgct gatttgcttt catctaaatc
     7141 agcaagttca actgcaaatg gattttcaat agcacggata aagtctgtta gtgcatcagc
     7201 atcagctggt ggtagttgac ctccgtcttg tccatagact ttataaccat tgaaaggtgc
     7261 tggattgtgg cttgcagtaa ccataatacc agcataggca tttaaatggc gaacagcaaa
     7321 agaaagttct ggtgttggac gaagagtttc aaatacgtaa gatttaattc catgctgtgc
     7381 caagacctgt gcagattcaa aagcaaattc tggagagaag tggcgcgaat cataagcgat
     7441 ggctacacca cgttttttag cttcctctcc cttagtttcg attaattttg ctaatccttc
     7501 ggttgcttga cggacaacat aaatattaat acgatttgta ccagcaccaa tataaccacg
     7561 cataccagca gtaccaaatt ctaagtttgt gtaaaaagca tcttcttttg atttttcatc
     7621 cattgatagt aattcttgac gaagatagtc aggaagctgc tcaaaatcta accatttttg
     7681 aaaattttct gtgtaagtca ttgaaat
//
LOCUS       DAUELZ010000078         7527 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.079, whole genome shotgun sequence.
ACCESSION   DAUELZ010000078 DAUELZ010000000
VERSION     DAUELZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.079"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..3153)
                     /gene="carB"
                     /locus_tag="VHI09_001307"
     CDS             complement(<1..3153)
                     /gene="carB"
                     /locus_tag="VHI09_001307"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEQ6121183.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPALDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQELQMHVDHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLIEYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSILIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESR"
     gene            complement(3399..4481)
                     /gene="carA"
                     /locus_tag="VHI09_001308"
     CDS             complement(3399..4481)
                     /gene="carA"
                     /locus_tag="VHI09_001308"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEQ6121184.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            complement(4525..5460)
                     /locus_tag="VHI09_001309"
     CDS             complement(4525..5460)
                     /locus_tag="VHI09_001309"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEQ6121185.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKESYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            complement(5521..6780)
                     /locus_tag="VHI09_001310"
     CDS             complement(5521..6780)
                     /locus_tag="VHI09_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEQ6121186.1"
                     /translation="MKDVIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKVIGNDWIDKILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            complement(6796..7317)
                     /gene="pyrR"
                     /locus_tag="VHI09_001311"
     CDS             complement(6796..7317)
                     /gene="pyrR"
                     /locus_tag="VHI09_001311"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEQ6121187.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
BASE COUNT         2345 a         1639 c         1252 g         2291 t
ORIGIN      
        1 tctgctttct aaaaccgtta acatagcttt tgcagtatct aaagctgtaa ataaaggaac
       61 tccttgttca atagctgaac ttctaatcat ctgtccgtct ttatcagctg tgcgtttaat
      121 tccaactgta ttaacaattg cctgaacgtg accatgtcgc acgagtgtcg ggatgtcatt
      181 ggcagcatca ccaatttttc caactaaaca cgcgcttaag ccttgctcag caaagtaagc
      241 tgctgtacct tgagtagcca taatttgata gccaatcgcc ttaaatcgtc ttgctagact
      301 tagagcttct gccttactat catctgctat agtaaagaca atttgaccaa attctgataa
      361 atgagaatta ttggcttcaa acgctttata aagtgctttt tcaagactag tatccgaacc
      421 cattacttct ccagttgatt tcatttcagg acctaaaaga ctatcaacct gtgccaactt
      481 tgtaaatgaa aacacaggcg ctttgatatg caccagttga ctttggggat aaagaccatc
      541 ttcatatcct agatccttta atgtttgacc taaaatcaac ttagtagcaa tctgagccat
      601 cggaatacct gttacttttg ataagaaagg cacggtacga ctagcgcgtg gattcacttc
      661 aataacatag acttgttcat ttttaataac aaattgaaca ttcatcatac caatacaatt
      721 taacccaatg gctaaacgtt tagtatactc agctattgtt tcttgaattt gcttagataa
      781 ttgctgtggc ggataaactg ccattgaatc tcctgaatga acacctgctc tttcgatgtg
      841 ctccataata ccaggaatta agattgattt tccatctgaa atggcatcca cttcacactc
      901 cttaccgaaa atataagagt ccactaagat aggatgttca ggacttgctt taaccgctgt
      961 tcttatatat tctataagat cttctttgtt ttcaacaatt tccatagccc ggccaccaag
     1021 tacgtaagaa ggtctaacta agactggaaa accaattttt ttagctgcct ctaaagcttc
     1081 ttcttcatta gttgccgttt gaccttgtgg ttgtgggatt ccaagctctt tcaacgcctt
     1141 ttcaaataaa tctctgtctt cagccctatc caaatcctct acttgtgttc ctaatattgt
     1201 aacacccgcc tcagaaagtg cttgtgctaa gttaatagca gtttgtcctc caaactgaac
     1261 tattactccc ttaggctgct ctaaatctat cacattcatg acgtcttcaa aggttaacgg
     1321 ttcaaaatat aatttatctg ataccgaaaa atctgtagat acagtctccg gatttgaatt
     1381 catgataatg gcttcatagc cagctttttg gatggcttta acagaatgaa ccgttgcgta
     1441 gtcaaattca accccttgac caatacgaat agggcctgaa cctaaaacta aaattgattc
     1501 tttattcgac tgaacagatt cattttccaa ctcatatgtt gaataaaaat aaggggtttt
     1561 agcatcaaat tcagctgcac aagtatctac cattttatag acaggataca ggctatgaga
     1621 atgtctcatc gctctgatat cactttcatc cttttgccaa atttctgcaa ttttttgatc
     1681 tgaaaatcca taacgctttg cttttttgag gctttccaaa tggtcaacat gcatttgtaa
     1741 ttcttgctct atttcaacaa tatgaagtaa tttatccaag aaaaataaat caatcttggt
     1801 caagctctca agttcttcga tactataacc tcgtcgtatt gcttctgaca agtaaaaaag
     1861 tcgatcatct tgagctttaa tcactttagt cactaactct tcatcactaa tgttacttaa
     1921 acttgtcatc tcattatggc aaacaccaat ttctaaagaa cgacaggctt ttagtaagga
     1981 ctcttccaaa ttacggccaa tagccatgac ttcacctgtt gctttcattt gagttcccag
     2041 ttggcgttca ccatgttcaa atttatcaaa cggaaaacgc ggaattttag caacaacgta
     2101 atcaagggca ggttcaaaca tagcgtaagt agtgcctgtg atagggttaa tcatttcatc
     2161 aagtgttagg ccaacagcaa tctttgcagc caatttggca atgggataac ctgttgcttt
     2221 tgaagcaagg gcagaggaac gagacactct aggatttact tcaataacat aatatttgaa
     2281 actataagga tctagtgcta actggacgtt acagccacct tcaattttca gagctcgaat
     2341 tatttttaaa ctagcatcac gtaacatttg attttcaata tcagaaaggg tctgtgttgg
     2401 tgcaaataca atagaatctc ctgtgtgaat ccctacggga tcaaagtttt ccatattaca
     2461 aacaaccagc gcgttatcag cactatctcg cattacttcg tattcaattt ctttgaagcc
     2521 agcaattgag cgttcaatca aacactgtgt gactggtgat aactttaggc cgttttcagt
     2581 aatttcacaa agttcttctt cactactaca tattcctcca ccagttccac caagagtaaa
     2641 agctgggcgc acaatgactg gataaccgat atctctagca aatgtaacag cttcatcaac
     2701 tgttttaaca attgtagatt ctggaatggg ttggtctagt tcttgcatta attgtttgaa
     2761 aaggtctctg tcttctgcct gatcaatcgc agataactta gtacctaaca attcaatctc
     2821 taaatcatca agaataccag ctttagataa cgccattgcc atattgaggc ccgtttggcc
     2881 acctaaggtt ggcagtatgg catctggcct ttcttttcga atgatacgat taacaaattc
     2941 aagagtcaaa ggctcaatat ataccttatc agcgatttct ttatctgtca taatcgttgc
     3001 aggatttgaa ttaacaagta tgactttgta gccttcttct tttagagcca aacaggcctg
     3061 agtccctgca taatcaaatt cagcagcttg tccaataata atcgggccag acccgataac
     3121 catgattttt tgaatatctt ttcgttttgg cataattaat gataccaaga ataccacaag
     3181 aagcggtaaa gtcagcactt ctcaataaga gctttatctc tttgaaaacc taacttttta
     3241 ctaaattatt tacttgtgtt ctccttgcct tcctttctct atttgttgcc tcacagagca
     3301 acattaaata atatttttga tagggtacta aatcaactgt aaatgttaat aaatagagta
     3361 acaagaggtg atatagtcgt gaaattgcaa ctgatttact atttactcgt acatctccaa
     3421 gaatcaatca tttccaaaaa ttcgtcaaat aagtagctgg catcatgagg tcctggagcc
     3481 gcatcagggt gaaattggac tgaaaaagct ggaaaatctc tatgtttcac tccttcgacc
     3541 gtcttatcgt taatgtcttc atgcgtaacc attaaagtat caggtagact actacgttca
     3601 acggcataac catggttttg actggtaaaa tcaattcttc ccgttgcaat ttctctaacg
     3661 gcatggttaa agccacgatg accaaaggtc attttacaag tcttagcacc atttgctaga
     3721 ctaaagagtt gatgtcccat acaaatacca aaaataggaa tcttaccttg taccccacga
     3781 atcatgtcta aagcctctgg caaatcttct gggtttcctg ggccattaga tagcatcaag
     3841 ccatccggat ttagttggag cacttcttct gcagtgatgt tgaaagggac aacggtgata
     3901 ttacattgtc ttttagaaaa ttctcttaaa atagaatgtt ttaaaccaaa atcaaccaat
     3961 acaatatttt tcccgatccc tggtgcagga taagctgttt tagttgacac ttgttcaatg
     4021 gtgttagtcg gtaagactgt tgcacgcagt tgatctttta aatgctggat actatctcca
     4081 tcatctgcca tcgtagcttt catggtaccg tgttgccgaa taattttggt caaagctctc
     4141 gtatcaattc ccgaaatccc tggaatccct tttattttca agaacgcatc tagcgtcatt
     4201 tgcttgcgcc aattacttgc caaccgactg acttcggaca caacaacccc tttacaggtg
     4261 ggtgaaatgg actcatagtc atctcgatta atcccataat ttccaatcaa aggataggta
     4321 aaggttaaaa tttgcccatt gtaggattgg tcagtgatgg attcttggta gcctgtcatt
     4381 cctgtattaa agacaatttc accagtcaca tcaatatcag caccaaaggg ttctccttca
     4441 aaaatagtgc cgtcttctaa aataagtaat cgttttgtca ttttcctacc ttttcctacc
     4501 ttctaaacat gctttaatct ttcatcatga gtttttattt ctaccattca aaatggcttc
     4561 aataatagcc atgcgaacaa agacaccatt agccatctga gatacaattc tcgctttagg
     4621 agcttccacc aaactatctg caatttccac atcccgatta actggagctg gatgcataat
     4681 aatagcagaa tcttttaatt gttggtaacg ttcctgtgtc aatccaaacg cttgatggta
     4741 actttctttt gaaaaactct gatggccgtc atgacgttcg tgctgtacac gcagcaacat
     4801 aagaacatcc aattccttaa taatctggtc aatagcaata taagtgccgt aagcattaaa
     4861 ggcttctgaa taccattctt caggtccata gaaataaagc tctgctccta atcttttgag
     4921 gatttgcatg ttggactttg ccaccctaga gtgagttaag tcaccagcga tcgcaatctt
     4981 taagccttca aagcgtccaa attcttcata gatggttaat aagtcaagta aacattgact
     5041 cgggtgttgt ccagaaccat ctcctccatt gacaatggaa gctgtaatag ttggactttc
     5101 tactaattcc ttgtaataat catcttctgg gtgacgaatc acacagatgt ctgttccaag
     5161 tgcactcatg gttaacacag tatcatacaa actttctccc ttgttaaccg cacttgcatc
     5221 agcattgaaa tcaagaactg tcaagccgag tttcttctca gccacttcaa aagacttatg
     5281 ggttcgggtg gagttttcaa agaataaatt ggcaacaaga tccttttgat gatccgaaat
     5341 aacaacctta ccagccttat attctgaacc acggttgata agacctaaaa cttcttcggt
     5401 ggttaatgct tccattgaaa ctaaattggt aagtgcaacg cgattattta caactgacat
     5461 gattatcccc tttatcacta acgactgatg actatgtaag tagtctaaca acaattcttc
     5521 ctatttttca ggaagtacct gatataagat gatgccaaga agtgttgaga aagcgacacc
     5581 tgaaatttga agaccattta cttgaagcat caatcctcca atcccagaga ccaaaatgac
     5641 actggcaatc aataagtttt tcttattatc catatcaact ttggattcaa tcaagatttt
     5701 caaaccgcta gaagctatca caccaaacaa ggcaactgat atacctccaa taactggtgt
     5761 tggaatagat tgaattaagg ctgatacctt accgataaaa ctgaggagag cggcaatcac
     5821 tgcggcacct gcaataacat agaccgagaa tattttattc agagccatta caccgatgtt
     5881 ttcaccataa gaagtgaccg gaggtgcccc taagaagccg gcaatgattt gcgcaaaacc
     5941 atcaccagtt aaagtctttt caagtcctgg atccttgaaa taatctcttt ttgtcaaact
     6001 atttaagacc ataatgtgtc caaaatgttc tgtcattgta acaaaggcaa tgggtgccat
     6061 agttaaaatg gcacttgggt agaatttaac cccgtaggtt aagaaaggaa tttccactga
     6121 tgggatacta aaccatttgg cctgagccac attggtaaaa tcaacaattt cttgaccggt
     6181 gagaacaccc actaaaaggg ctacaacata accaactaat agccctaata aaagcggtac
     6241 aatagcaaca attcccttac cataaatatt gaaaaaaata actgataata aggtgactaa
     6301 cccaataaca agataagtca gattatagtt cccatttttt aacatgacat cattaacagc
     6361 tgtagaagct agacttagac caatgaccat aacaatcgga ccaactacaa tcggtggtaa
     6421 aatcttatca atccaatcat tgccaataac tttaacaatc aaagctacaa tcaggtaaac
     6481 gagacctcct gtcatagccc cttgagctac agcaccaata ccatttgtct tcatcagtaa
     6541 ctgcatagct gctatataag caaaacttga gcccatgtag gctggaattt taaatttggt
     6601 taccgataaa tgtgcaagtg ttcctaaacc actcgatagg agggccacag acgggtcaat
     6661 tccaactaaa ataggaacaa gaaccgtcgc cccaaacatg gcaaataaat gctgaaagga
     6721 caaaccaact aacatacctg cctttggcac ttcttccaca tcataaatga catctttcac
     6781 agtaaaacct ccttcttatg ttgggtcaat aatgctaaca cgatcacggc catcaacttc
     6841 gacaacctca acaacaattt cttctacact gcttgttgga atatttttcc caacgtaatc
     6901 cgcgcgaatc ggtaattccc gatggccacg atccaccaaa acagctagac taacacgggc
     6961 tggtcgacct aaactcacta agttatcgat ggccgcgcgg atggtacgac cagtataaag
     7021 cacatcatca atcaaaatca catccttgcc tgtaatatct actgacatca gagttgtgtc
     7081 ttcctcaact ctcatgtcat ctctaaaagg cttgatgtct aactcaccaa ttggtaaatc
     7141 caacccttct aattgatgca gtctttcttg aatgcgacga gctagaaaaa cacctctcgt
     7201 tttgatgcct gccaaaacca cattgtctaa ttgtttatta cgttcaataa tttcataagt
     7261 aatacgggta atggcgcgtt tcatggtcac gtcatcaaca atttccttag ttttcatgtt
     7321 gaaacctcct ctaaaaattc aaataaaaag atctccttgg aaacaaggag attcagcaaa
     7381 atatagcaca attaagtatc atagtgcatg tttttaatcc gtctccttga cagcctcaca
     7441 ggactgtttt aaaggaatat tttatttcaa tcagtatagc aaaaaaagaa aagaaaaaca
     7501 agtaaaaaag acaataccta gcatcta
//
LOCUS       DAUELZ010000079         7467 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.080, whole genome shotgun sequence.
ACCESSION   DAUELZ010000079 DAUELZ010000000
VERSION     DAUELZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.080"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..819
                     /locus_tag="VHI09_001312"
     CDS             <1..819
                     /locus_tag="VHI09_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEQ6121188.1"
                     /translation="LLSLFADPWLLAVHLLDILIVAYLIYRFIKALTGTKIMSLVQGV
                     IFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEKFGRSTQVFLQK
                     QYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPLNADISSQLLIN
                     IFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAIGLSENSDALTI
                     IVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKKILGGKSK"
     gene            816..1772
                     /locus_tag="VHI09_001313"
     CDS             816..1772
                     /locus_tag="VHI09_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEQ6121189.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVSVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVNKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            1826..3181
                     /locus_tag="VHI09_001314"
     CDS             1826..3181
                     /locus_tag="VHI09_001314"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEQ6121190.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            3305..3946
                     /locus_tag="VHI09_001315"
     CDS             3305..3946
                     /locus_tag="VHI09_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121191.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTQKEDT
                     QNRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLAD
                     MQEKLPSLSIMVSQESSKLFYKEQGFVLLAKGCKPGNENDSDVCQLVKYFSKP"
     gene            4009..5139
                     /locus_tag="VHI09_001316"
     CDS             4009..5139
                     /locus_tag="VHI09_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEQ6121192.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLRALIQE
                     FDSYGIRDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKAAGFHNISIDLIY
                     ALPGQTLDQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGMPFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            5149..5901
                     /locus_tag="VHI09_001317"
     CDS             5149..5901
                     /locus_tag="VHI09_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEQ6121193.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGC
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKLHRAPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCEFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQITSDGQLNAQAMIEWRAIKQTESETD"
     gene            5901..6665
                     /locus_tag="VHI09_001318"
     CDS             5901..6665
                     /locus_tag="VHI09_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEQ6121194.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQEKGIPYLLVTNN
                     TTRTPEMVQSMLANQFHVETGIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            6665..7297
                     /locus_tag="VHI09_001319"
     CDS             6665..7297
                     /locus_tag="VHI09_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEQ6121195.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
BASE COUNT         2282 a         1325 c         1595 g         2265 t
ORIGIN      
        1 ttattaagct tatttgcgga tccttggctg ctggcagtgc atcttttaga tattctaatt
       61 gttgcctatt tgatttatcg ttttattaaa gcattgactg gaactaaaat catgtcttta
      121 gttcaagggg tcattttctt tttggttctt cgttttattg cagagtggat tggctttacg
      181 acaatcacat acttaatgaa ccaagtcatt acttatggtg taattgcagg agtagtcatt
      241 tttacccctg aaattcgggc aggactggag aaatttggtc gttctacgca agttttttta
      301 caaaaacagt acgtcagcag tgaatctgct ttggtcgatg ctttgattaa atctgtggcc
      361 tatatgggtc cacgcaagat aggtgcattg attgctattg aacagactca aaccttacaa
      421 gaatatattg cgacaggaat tccgctgaat gccgacattt caagtcaact actcatcaat
      481 atttttattc cgaatacgcc gcttcatgat ggagctgtta tcgttggtca gaataagatt
      541 gtggcagcct gtgcctactt gcctttgtca gaatcaaagg caatttccaa agaatttgga
      601 acccgtcata gagcggctat tggattatct gaaaactctg atgcactaac tatcattgtt
      661 tcagaagaaa caggagctat ttcagtaact cgcaaaggcc aatttttaca cgatttaagc
      721 acagatgaat ttgaaacggt acttaggacc tacctgatga gtgattcaaa tgttagttta
      781 ccatggtata agaaaattct aggagggaaa tctaaatgaa acgattttta aatagtcgtc
      841 cttggttagg aatggtatca gtcttttttg cgattctcct ttttttaact gcagcatcga
      901 gcaatcataa taattctagc tcgcaaattt acagcccgat tgaaacctat acgcatagcc
      961 ttaaagatgt accaattgat atgaagtatg atagtgataa atattttatc agtgggtatt
     1021 cttatggtgc ggaagtttat ctaacctcca ctaatcgtat caaattagat tctgaagtga
     1081 acaatgatac acgtaatttt aagattgtgg cggatctaac acatagtcat ccaggtacag
     1141 taagtgtcaa cctaagagta gaaaacctcc cttctggggt gacagctacc gtatctccag
     1201 ataaaatatc agtcacaatt ggcaaaaaag aatctaaggt atttcctgtt agaggaagcg
     1261 ttgatgctaa acaaattgca aatggttatg aaattagcaa aattgaaacg ggggttaata
     1321 aggttgaagt gaccagtgat gaatctacca ttgctttaat tgatcacgtt gtggcaaagt
     1381 tacctgatga tcaagtgtta gataggaatt atagtagtcg agtgacctta caagctgtct
     1441 cagctgatgg aactatttta gcaagtgcaa ttgatcctgc aaaaacaaat ttatcagttg
     1501 ctgtaaaaaa aataacgaaa tcagtcccga ttagagttga ggcagtgggg atgatggatg
     1561 atagcttatc agatattcaa tacaaattgt caaaacaaac agctgttatc tcaggtagtc
     1621 gagaggtctt agaagatatt gatgaaatta ttgcagaagt taacatttct gatgtcacta
     1681 aaaatactag taagaccgtg agtttgtctt ctagtcaggt gtcaattgag ccgtcagtag
     1741 tgaccgttca gttgacaacc accaaaaaat aatggataat aactaggaaa attgaatgga
     1801 ggaagccatt aggcttatat aaattatggg aaaatatttt ggaacagatg gtgttcgtgg
     1861 tgaagcaaat gttgagctga cacctgagtt agcttttaaa ctgggccgat tcggtggtta
     1921 tgttttgagt caacatgaga ccgaaagacc taaagttttt gtggcgcgtg acacccgtat
     1981 ttcaggagaa atgttggagt cagccttgat tgctggtcta ctatctgttg gtattgaagt
     2041 ttataaactt ggtgttttag cgacacctgg tgtctcttat ttagttagaa ctgaaaaagc
     2101 aagtgcaggt gtgatgattt ctgcaagtca taaccctgcg cttgacaatg gcatcaaatt
     2161 ctttgggaat gacgggttta agttagctga tgaccaagaa ttggaaattg aagctctctt
     2221 agatgctccg gaggacacgc ttcctcgccc gagtgcagaa ggacttggaa ccttggtaga
     2281 ttatccagaa ggacttcgta aatatgaaaa gttcttggta acaacaggta ctgacttaag
     2341 cggtatgaca gttgccttag acactgctaa tggagctgct tcagtgtcag cacgtgatgt
     2401 ctttttagat ttaaatgcag aaattgctgt cattggcgaa aaaccaaatg gtcttaatat
     2461 taatgacggt gttggttcaa cgcatccaga acaacttcaa gagttagtta aggaaacagg
     2521 agcagatctt ggtttagcct ttgatggcga tagcgaccgt ctgattgccg tggacgaaac
     2581 tggcgagatt gttgacggtg atcgtattat gtttatcatt ggtaaatact tgtcagaaaa
     2641 agggcttctt gctcacaata ccattgtcac aacggtgatg tctaacctcg gttttcataa
     2701 agctttagat aaacaaggta ttaataaagc aattacagca gttggagacc gctatgtggt
     2761 tgaagaaatg cgtagctcag gatacaatct cggtggtgaa caatcaggcc atgtcattat
     2821 catggactac aataccactg gagatggtca gttgacagct atccaattag ccaaggtgat
     2881 gaaggaaact gggaaatcac tttcggaatt agctgctgaa gtaaccattt accctcaaaa
     2941 actggtgaat attcgtgtgg aaaacagcat gaaagaccgt gctatggaag ttccagctat
     3001 tgcgaacatc attgcaaaaa tggaggatga aatggctggt aatggtcgta ttctcgtgag
     3061 accaagtggt acagaacctc ttcttcgtgt catggcagag gcgccaacag atgccgaagt
     3121 tgattattat gtcgatacta ttgctgatgt cgtccgtaca gaaatcggat gcgataacta
     3181 acgagttgag tacaaacaag aaaccttatt tctaaggctt ctgtcaacaa ttggaaagag
     3241 gcttggggcg tttagcccaa gtctcttttt ataaattgct aaagtagaag agaaaggata
     3301 tcggatgaca aaacagcaaa tcgaaaaact agcggactta ctgcctcctt ttgcagccac
     3361 actttactta gatatgattc ctgaatttca ggaaggttat gcttcatatg tttatcaaac
     3421 ccaaaaagaa gatactcaaa accgtcgcat caagcagtta actaaaaatt ttgaacgatt
     3481 actagatacc ccatcttttt atgtgatgcc tgtgacaaga aaaacagcag agcaattgct
     3541 agcagataaa ggcctagctt atcatttgtt atcgacagtt gcagctaatc aagtgtttgc
     3601 ggctgctgtt aacgaggatc agacgctttt tcaagtactt cgaaatggag caagagtagc
     3661 ctttgtgatt cttcaagaaa agggggaatt tctagaaatg actttaggga ttcttgaaac
     3721 agtgacagaa aacgaggttc cagcagctat tgtggaggct gtccagacat atcttctggc
     3781 agatatgcaa gagaaacttc cttctttaag cattatggtc agtcaagaat caagcaagct
     3841 tttttacaaa gaacaagggt ttgttttgct agctaagggt tgcaagccag gaaacgaaaa
     3901 tgattctgac gtttgtcagc tcgtaaaata cttttccaag ccatgaagaa tcaagggaaa
     3961 tggtttattc ataaaaacat tttatcacca aagtgatata ataagaccat gtcaaaaaaa
     4021 cctacttcag cctatgtgca cattcctttt tgcacacaaa tttgttatta ttgcgatttt
     4081 tcaaaagtct ttatccaaaa tcaacctgtt gatgcttatt tgagagcctt gattcaagaa
     4141 tttgactctt atggcattcg agacctaaaa accctttata ttggaggagg tacgcctaca
     4201 gctatcactg ctaaacagtt ggagtatctc ctaaatcacc ttgaacgaaa cctcaattta
     4261 gatgatcttg aagaattcac aattgaggca aatcctggtg atttgacgcc agaaaaaatt
     4321 gcggtgcttc agaggtcagc tgtcaatcgg atttccttgg gggtgcagac ttttaataac
     4381 aagcaattaa agcagattgg ccgtagccat aatgaagaac aaatttatag caccatcgct
     4441 aatttaaaag ctgcaggctt tcacaatatt tccattgatt taatttatgc cttgccaggt
     4501 caaaccttag atcaagtcaa ggaaaatgtt gctaaagctt tggcacttga tattccccac
     4561 cttagtcttt atagccttat tttggaacat cacactgttt ttatgaacaa aatgcggcgt
     4621 ggtaaactta acttaccaac ggaagacttg gaagcagaaa tgtttgagta tattatttca
     4681 gaaatggaag ctaatggctt tgaacattat gaaatctcta attttaccaa accaggtttt
     4741 gaaagtcgcc ataacttgat gtattgggat aatgtggagt attttggatg tggtgcaggg
     4801 gcttcaggtt accttaatgg cattcgttat caaaatcggg tgcccatcca gcattatctt
     4861 aaagcggttg aagcagggaa tgctagacta aatgaagaag tgctgcgcaa agaggaaatg
     4921 atggaagagg aactcttttt gggcttacgt aaaaagacag gagtctccat ccagcggttc
     4981 caagaaaaat ttggtatgcc ttttgaggag cgttatggaa atattgttag ggagcttcaa
     5041 aaccaaggac tgcttgtcaa agatgacgcc tttgtcagaa tgactaaaaa gggtcttttc
     5101 ttaggtgatt ctgttgctga gcggtttata ttagattagg agagtattat gggattaagt
     5161 tatcaggagg agttgacact tccttttgaa ttatgtgatg tcaaatcaga tataaaattg
     5221 ccccttttat tagactattg tttgatggtt tctggtagac agtctgcgca attaggacga
     5281 agtaacaaca accttttagt tgattacaag cttgtttgga ttgtaacgga ttatgagatc
     5341 actattcatc gcttgccaca ttttcaagaa accatcacca ttgaaacaaa agccctttcc
     5401 tataataaat ttttttgtta tcgccaattt tatatttatg atcaagaggg gtgcctttta
     5461 gtggatatct tagcctattt tgctttgtta aacccagata cgcgaaaagt ggcaactatt
     5521 ccagaagatt tagtagcgcc ttttaagact gattttgtta aaaagctaca ccgtgctcct
     5581 aaaatgcctc ttttagaaca atcaattgat cgtgattatt atgtgcgtta ttttgatatt
     5641 gatatgaatg gtcatgtcaa caacagtaaa tatttagatt ggatgtatga tgtgttgggg
     5701 tgtgagtttt taaaaacgca tcagcctctt aagatgactt tgaaatatgt taaagaagtc
     5761 tcaccaggcg gtcaaattac ttccagttac catttggacc aattaacttc ttaccatcaa
     5821 atcacctcag atgggcagct gaatgcccaa gccatgattg aatggcgagc gattaaacaa
     5881 acagaaagtg agacagacta gtgccttata agggttattt aattgattta gacggaacga
     5941 tttaccaagg gaaaaaccgt attccagcag gggagcggtt tatcaaacgc ctacaagaaa
     6001 aaggcattcc ctatttatta gtgactaata ataccacaag aacacccgaa atggtgcaaa
     6061 gcatgctggc taatcagttt catgttgaga caggcattga gaccatttat acggctacca
     6121 tggcaaccgt cgattacatg aatgacatga accgtggcaa aacagcttac gttattggag
     6181 aaacagggct caaatcagct attgcagcag caggttacgt agaagaactt gagaatcccg
     6241 cttacgtggt ggttggttta gacagtcagg tgacctatga gatgttggct atagccacct
     6301 tggctattca aaaaggtgcc ctctttattg ggacaaatcc tgatttaaac attccaaccg
     6361 aacgtggttt gatgccagga gcaggtgcat tgaatgcctt actagaagct gccacacgtg
     6421 taaaacctgt ttttattggc aagcccaacg ctatcattat gaataaatca ctagaggtat
     6481 tgggcattca gcgctctgag gctgtcatgg ttggcgataa ttacttgact gatatcatgg
     6541 caggtattca aaatgatatt gcaacgatcc tcgtgacaac aggctttacc agacctgaag
     6601 aggtaccaac cttaccaatt cagccagatc atgtcctatc tagccttgat gagtggaggt
     6661 tgtgatggta gaaaacacta aattactatg tagctgggta tggttattag cgttagctat
     6721 tctcataacg atctattcaa cgtggctgtg gtatcctctt gaggtggacc atctaaagtt
     6781 agaacaagtt gtttttatga gcaaggatgc tatcttgcat aactataacg gactactaaa
     6841 ttacctcacc aatccttttg tgacaaggtt ggaatttgct aattttcact catcagcaga
     6901 cggactcaag cattttgcag atgtgaagtg gctttttcac ctaactcaag tcgtttttct
     6961 tggcctcctt tacccaaccc ttaagacttt cacacaaagg ttaaaaacca aacgtttctg
     7021 gctccttcaa aaacctttaa tactagcagc tttgtttcct cttatgattg gtctaatggc
     7081 tagtttcatt gggtttgaac atttttttac tctttttcat caggtgcttt ttgtaggaga
     7141 tagcagctgg ctctttgatc ccttgaaaga tcctgtgatt tggatccttc cagaagtctt
     7201 tttcttgcac tgtttccttt tctttatgat agtttacgaa atcatcctgt ggagcttagt
     7261 aggtttagca agatggcagc gcctaaatgt agaatgataa aaggattaag agattaattt
     7321 ccctaaaaat gataaaacaa gcgttttgaa agcgcttgtt ttttagtttg cagtcagagt
     7381 agaatagaag tatcaaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac
     7441 tagccttatt ttaacttgct atgctgt
//
LOCUS       DAUELZ010000080         7420 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.081, whole genome shotgun sequence.
ACCESSION   DAUELZ010000080 DAUELZ010000000
VERSION     DAUELZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.081"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            771..860
                     /locus_tag="VHI09_001320"
     CDS             771..860
                     /locus_tag="VHI09_001320"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ6121196.1"
                     /translation="MIIDYCNNKRIKIKLKGLSPLQYKTKSFT"
     gene            complement(898..1821)
                     /locus_tag="VHI09_001321"
     CDS             complement(898..1821)
                     /locus_tag="VHI09_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121197.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1814..2884)
                     /locus_tag="VHI09_001322"
     CDS             complement(1814..2884)
                     /locus_tag="VHI09_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121198.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2893..3819)
                     /locus_tag="VHI09_001323"
     CDS             complement(2893..3819)
                     /locus_tag="VHI09_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121199.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3819..5321)
                     /locus_tag="VHI09_001324"
     CDS             complement(3819..5321)
                     /locus_tag="VHI09_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121200.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYEKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMVGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5386..7365)
                     /locus_tag="VHI09_001325"
     CDS             complement(5386..7365)
                     /locus_tag="VHI09_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ6121201.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTVGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKVEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDSAVDIPVVALGGTPRVTKAVPFSGAFAWAGAKGPASVYYYKGMKLQDK
                     PVTAKQFEKAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2298 a         1445 c         1364 g         2313 t
ORIGIN      
        1 tttggttcgt cttattcagt tctcaaaggt cttaacgtct cctaagagac aacttcttta
       61 ttctatcagg tttctcatag cctgtcaact actttttcat cttttttcaa taagtgtgtt
      121 acaggaacct ctctgacagc tttattagta tacgatatct ttactgtcat tgtcaactct
      181 ttttttactt tttttgtact tttttaatat cccgcgttgt aaaatgaagt gcaacaaaaa
      241 agaggaaaag ccttgaacta caccccaaac gttagacaaa aaaatctaac gttcggggta
      301 ttttttatga aattaagtga tgaaggtacg aaatagacat cgttaagaaa gaaaaaaata
      361 cctactatcc accagagata aagcaggaaa taatggataa cgttttgata gaaggcccgc
      421 cacaatacca agtatcactt gtttatggct tgttacatgc aggaactctc ccaagttgaa
      481 tagcacaata taagaaaaac agttatactg ttgagaaaac aagagcaaga ccatcacaaa
      541 caggacgtaa accaaaagaa acctgaagaa atgactaaat tagagcggct tcaagaagaa
      601 aatgaatatc tcagaactga ggttgcttac ttaaaaaaac cgaggaactc cgtttgaaag
      661 aagcatctct gcaaagaagg agacaaaaaa accattataa aaaatggaca aagacgagct
      721 ctgattagat tttcttttaa gaacggctgt attgactcgt tctacctact attatcatcg
      781 attattgcaa caataaacgt atcaaaataa aactaaaagg acttagtcct ctacaataca
      841 aaactaaatc ctttacttaa aatagagtag ccaacttttg gggtcagtgc atctctttta
      901 caattctttt tgatattttt caatttctgc ttggtttgcc caaacaaagt gatttggttt
      961 gatttcaacc attgatggct tatctaatgt ataatcatgc tggtctggat gatagacaac
     1021 aagttctttt tgacgctcta aaattggatc tgggataggc acggctgata acaaagattg
     1081 ggtgtaggga tgaattgggt tattaaacag ttcttctgtt tctgcaactt ctacaataac
     1141 ccctttatgg ataaccgcaa tacgatctga aataaagcga acgactgaaa gatcatgggc
     1201 aatgaagaga taagtcaaac ctttttcggc ttgcatccgt ttgagaaggt ttaaaacctg
     1261 tgcgcgaacg gaaacgtcca aagctgaaat cggttcatca gcaataacga attcagggtt
     1321 cattactagg gctctagcga taccgatccg ctgacgttga cctcctgaaa attcatgtgg
     1381 gtagcgcgtc aaatgctctg atagcaaacc aacttcggcc atcatgttct taattttttc
     1441 tttacgttct tcctctgttt taaacagatt aaagttataa agaccttctg aaatgatgta
     1501 gtcaacggtt gctcgttcat tcaaactagc agcgggatct tggaaaatca tttgaatttt
     1561 acgaatgagc tcattggctt ctgattttga ttttctgcca ttaattactt tcccatcgta
     1621 taaaatttga cctgaactag tatcgttcaa accaataata gcacgaccaa ttgttgtttt
     1681 accactccca gattctccaa ctaaagaaaa ggtttctcct tttttaataa agaaattagc
     1741 atttttaact gcaacaaatt ttttctttcc ttctccgaag gaaatttcta ggtcttttac
     1801 ttcgactaat ttctcagaca tttccttcct cctgttgtga cattttcctt aagatttttt
     1861 gatgcaaatc ttgaatgact tctggttttt gaacttttgg agcctctggg tgtaaaagcc
     1921 atgttttggc ccaatgagtc tcagatacgt taatcgccgg aggtgctttt tcaaagtcta
     1981 aaaccatagc atattctgag cgaagtgcaa aggcatctcc gataattggt gaataaagtg
     2041 atggaggcgt tcctggaata gcgtaaagtt caccagattc atctgccaac tgcggtaagc
     2101 tagacagcaa actccatgta taggggtgtc ttggatcata gaaaatctct tcgactgttc
     2161 caaattcaac aatttctcct gcatacatga cagccacttt atctgcaatg cttgccacaa
     2221 cacctaaatc gtgcgtaata aagataatgg tgaaatgata ttctcgttga agcgacttca
     2281 ataactcaac gatttgagct tgaatagtca catcaagggc tgttgttggc tcatcacaga
     2341 taagaatatc tgggcgacaa gctaaagcaa tagcgataac aatacgttga cgcattcctc
     2401 ctgaatactc aaatgggtaa tcttcaaagc gttttttggc atttgggata cccactttat
     2461 tcatgtaatc aagggccatt tctttagctt tggcatgact tactttttgg tgcttaataa
     2521 tcacttctgt gatttggcta ccaatagttt taatgggact aagactggtc attgggtctt
     2581 ggaaaatcgt tgcgattttt gagccgcgaa tctttgccca ctctttattt gtttttaaat
     2641 ctgtcaattc ttgcccacga tagacaattg aaccattagc aatgcgtcca ttagactcca
     2701 acatccctgt aaaggtcttt gttaaaacag atttacctga gcctgattcc cctacaaaag
     2761 caaggacttc tccttcaaca agttccagtg agacgttacg gatagctgtt aaaacacgat
     2821 cacgcacatc aaattctacc accacatctt tggcagttaa gattacatta ttttcttttg
     2881 tcattttaac tcctatctat gtgaacgtgg gtcactggca tcagccaagt tttgtccgac
     2941 aatgtatagt ggtaacgata ctaaaatcaa tgttactaag ggaatccaaa agaggtaggc
     3001 atttgttgtt aagttgcttg aataattagc aatcaaacgt cctaaacttg gagtggtggt
     3061 tggtaaacca atcccaaaga aggataagaa ggcctcagaa gatacataaa ctggtagcat
     3121 ttgtgacaac atagtcatga taactgaaac caattgaggc agcaggttct taacagcaat
     3181 cttgtacatt ggtgttccca aagtttgact agcaaggttg tattctaaat cacggtaacg
     3241 caagatttga acacggatgg agtaggcgac accaatccat ccagtgatac agaaagctag
     3301 aatcaaattc caaaatcctg cacctaatga ataggtcaaa acaatgataa taagcataga
     3361 agggatattt gagataatgt tataaatttc aatcataact ttatcaaatg ctttagaaac
     3421 tccccatatg gctcctaaca ctaccccaat ggtgatatta attagtgtcg ctataactga
     3481 gattaaaata gaattacgtg ccccatacca aacaccatca aacagagatt gcccattttt
     3541 gtcggttcca aaccagtact ctgcatttgg ccaaatataa cgctttgaaa agtcattgat
     3601 attactaacg tcattaaagt cgtagttggc aaacattgga taaataaagc tcatcatcaa
     3661 gactgtcact aaaatcacga gcataaagac tgtagatttt ttagaaaaaa actgacgaaa
     3721 cactgatttc cagtaagaat aagcaggggt atcaatcact tcggaggctt cactatcgcg
     3781 ctcaacaaat cgaaatttag atttatcaat cgattccatt acttacctcc tttttttgtt
     3841 gacaatttaa tacgtggatc gacaagggtc atgacaatat ctcctagtaa aagtgaaaca
     3901 attgacaaga cagtgaagat aaaggttaat ccaacaatca tagaattgtt agcagattta
     3961 atagaatcaa ttaacatttt acccataccc gggaaggcaa agacagtctc tgttaatgtt
     4021 gcaccaccaa tagcaaggat aatacttgca ggaacccctg acacaattgg gaccatggca
     4081 tttttgaaga gatgccctct gtaaatttca gattctgata atccttttga acgcgcaaaa
     4141 cgtacccaat cgctggcctg aaggtcaaca agatagcgac ggaaccagat aaccgtagtt
     4201 ggaatattta aaattcctaa aataagggct ggtaaaatat aagatttagg atcagatgcc
     4261 cccagcattg ggaaactatc tggtaaacca accatgccac ctaggaagcg aacaacatag
     4321 ataacagcaa tacttggtaa agcaagcatg aaggtcatgg tcgctgttga gaaacggtca
     4381 aaataggtat ttttaaaccg agccataaat aaaccaagag gcaaaccaac gatataggaa
     4441 aacataacac caaaaaaacc aattacaaaa gaattgtgaa tcattgatgg gtcgacataa
     4501 ttattgatgg ttttagtgta ggaatctccc ttaccatagt ttaccttggt gatatcatcc
     4561 aatgattttg gatttttgta gctacggcta tagatatcca ctgacgattt cttagtgaca
     4621 cctgatggga aggtcacatc ttgaagagct gttcgtcctt gcccttgtga aataacctga
     4681 agaacaggaa tattgctgta tgttgggtaa gaggttccta agtttagggt aacaaaattt
     4741 tggtgaagat aaggaaattt tccgttagta tagaggagat atttatgttt tgtccccgaa
     4801 ccaacaagtg accagccaac tgatttatct ttttctagac gaatatacct agctaatttt
     4861 ggattatctt tatcctgaat cttccaaggg tgatcaataa caactagatt tgagaaaaag
     4921 ttccaaacac gttcgtaaat aggaatattg cgaattgcat aaaactgttt gctagtgggg
     4981 aagcgtttta gctgccagcc attgccaaga gaatcaatat acttctcata gatagctttg
     5041 ttagcactag ttgcttctgt cgttacacta ctatctactt tagaagcatt atcttttaat
     5101 tctttgcttg aaaaataatt gacataaccc atacgttgaa aagttagatt ttcatacgct
     5161 acttttttat ctggtgttgt tgtcatttta ttataattag ggtcttgttt aaagataagg
     5221 ctggtaggta ctagagtata tacgatggtg tatgttaaag ccgttaccaa aactaccgac
     5281 actaacgatc gcatgatacg gtttaaaatg tattttttca tagttatcct caatagttca
     5341 aaagaactct ctcacataaa cgagaaagtt cttttgaaca tcgttttatt tttcaacgtg
     5401 atcagctaac ttctcagcat attttgcatt tgacttagcc tttgctttca tccatttttc
     5461 ttttgctttt tcgaattgtt ttgctgtgac aggtttgtct tgaagtttca ttcctttata
     5521 ataatataca gaagcaggac ctttagcacc tgcccaggca aaggcaccgc taaatggaac
     5581 ggctttagta actcgaggag tgccacccaa tgccacaact ggaatatcta cggcactatc
     5641 tgtaaggtag gcttgtgctt ttgcgtaagc tttatagcgc gcatcgttgt cgtctgtaat
     5701 tgctgctgct tcatcaagaa gagtttggta agtatcaagg cctgcagctg ccacaacatc
     5761 cttattttga cctgctttga ttccaagttt ttggataaca gatccaccac ctactggact
     5821 catgatgtca aggtaggtcc gtggatcttg gtagtctggt ccccaccatg atgaaatgat
     5881 atcgtagtct tgttgttctg gggtctcagc atagaagcct tgggcttcgt gagttgatgt
     5941 ttctgtttca agaacattga caatgacatt ttctttacca agagatgctt caacagattg
     6001 tttgaaagac tgggcttcct gaacagttgc tgcgtttgcc tggtcaacag ggtaatctaa
     6061 ttgaactggg aaggttacac cttcagctgt caaagcttct ttggcttttg caaattcaac
     6121 ttttgctttt tctggattat agaaaccatc ttgagcatca gctaagttaa cgtctttcca
     6181 ttcatcacca agttttgcca tttccttttc aacttctgaa ccaaaatcac tttctccaac
     6241 ggttacaaaa gttggtggca caagcatgtt acgtaaggct tttgttttgg catcttgacc
     6301 tgcagtttgt gcttggaatg acgctcggtc aaaagcaaat tgaatagctt gacgaaaatc
     6361 cttgttgtta agagctttct taccggcatc ttgttgtgca gggtctttct tagtgttttt
     6421 gaaagaagta cggttcaaat tccatgttaa atgacggata tctccagtca acattccgta
     6481 agtaatgtta tcagcatagt ttttcttagc tgatttgtag gtagggtcat ttgggtaaag
     6541 tcgtgcaacg ctgaactcac ccttgtcaaa gttcttgtag aacgaacctg ggtctgaacc
     6601 atctgagtaa gtcaatttaa cagattctat cccaacattc ttagcatccc agtagttttc
     6661 atttttatgg aattccatag atgattttga ggtgaaggcg ctcaagaagt aagcaccatt
     6721 aacaaggatt gatgatggat cggttgtacc aaaatcttta ccttttgact tcaaaaattt
     6781 cgcattaact gggaaaagca cactataagt tgtttttgaa ttccagtagc tttcaggctt
     6841 gttcaaagta tactgaacag ttttatcgtc aagggctttg acaccaactt ctttaaaatc
     6901 tacttcacca ttttggtaag cctttaagtt ttttattgaa tcttcaacaa cgtaaagagc
     6961 atctgattta tcgtcaaccg cgtgcttcaa accagtcaca aaatcttctg ctgttactgg
     7021 ggcatattct tcaccatcag ccgtatacca agagacacca tcgcgaagag tataagtata
     7081 agtcagaccg tcttttgaaa ccttccaatc tttagcaagt gatggtacta aattaccata
     7141 ctcatcgttt tccaaaagac catcaaccat ttgtgttatc acatcagtcg ttccaccgcc
     7201 attagtcaaa atataatcca atgattttgg atcgttaaca aaaacgtact tgtaggtttt
     7261 tgtagcttct gagccacctg aagcattttt attaccacaa gctgccaaag cggatactga
     7321 caagatcgca acacttacag ctgccaacca tttacttttc ttcataaaag tcacccctaa
     7381 actttatttt tcattttttg gcaattgcta aatgaaattg
//
LOCUS       DAUELZ010000081         7366 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.082, whole genome shotgun sequence.
ACCESSION   DAUELZ010000081 DAUELZ010000000
VERSION     DAUELZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.082"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..707)
                     /locus_tag="VHI09_001326"
     CDS             complement(<1..707)
                     /locus_tag="VHI09_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121202.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGFAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAVLLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRL"
     gene            complement(710..1435)
                     /locus_tag="VHI09_001327"
     CDS             complement(710..1435)
                     /locus_tag="VHI09_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121203.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            1507..1926
                     /locus_tag="VHI09_001328"
     CDS             1507..1926
                     /locus_tag="VHI09_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ6121204.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKIARAIQSATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEEDGISIQYTTHEPDFPVLEKLARQINQEVSS"
     gene            1923..2279
                     /locus_tag="VHI09_001329"
     CDS             1923..2279
                     /locus_tag="VHI09_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEQ6121205.1"
                     /translation="MKVETVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLTEIQNRMAKYQEDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(2390..3673)
                     /locus_tag="VHI09_001330"
     CDS             complement(2390..3673)
                     /locus_tag="VHI09_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ6121206.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDSTASSH
                     TPTTPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(3682..4206)
                     /locus_tag="VHI09_001331"
     CDS             complement(3682..4206)
                     /locus_tag="VHI09_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ6121207.1"
                     /translation="MIQQSLVIEEGVPQDAKAVLSLVTAAAKETDFITGVETILEATP
                     QELSDFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQVEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDIYHMRKSVSQ"
     gene            complement(4181..4642)
                     /gene="tsaE"
                     /locus_tag="VHI09_001332"
     CDS             complement(4181..4642)
                     /gene="tsaE"
                     /locus_tag="VHI09_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEQ6121208.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHDSAKSRY"
     gene            complement(4796..6256)
                     /locus_tag="VHI09_001333"
     CDS             complement(4796..6256)
                     /locus_tag="VHI09_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEQ6121209.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSSVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIVVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCCSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            6520..7332
                     /locus_tag="VHI09_001334"
     CDS             6520..7332
                     /locus_tag="VHI09_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ6121210.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKTIKAVQDKGHHVIISTG
                     RPYRMALDYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLQYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTRHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
BASE COUNT         2486 a         1479 c         1230 g         2171 t
ORIGIN      
        1 agtcgtaaaa ataagcccaa ataatctgaa ctacgtaaaa aggctctagc ataaagggac
       61 agccaaaggt ttgaaggagt ttgcttaact aattttaaaa gaggatttag ataagttctt
      121 tctttcactt ttgtagaaat tcctttgact gttgtaaaaa gagaataaaa ctttaaaata
      181 ctttgcttgc gattgctttc aaatgcgaca gcttttgccc aatctaaacg atttcctctc
      241 aaaaaatatc ttgtctttcg actaagcact actcttttta gtgccaataa tatcaaaact
      301 aagataataa aaataaataa agacagacct aaacgtctaa aaataggata taaaaagagc
      361 aatacagcag tctgcaaagt tccccaaagc aaaaaagaga gtcgctcagc ttgtttgata
      421 taagaaatca cttcttcttc ttttggaagt aagaaatgct gatctgcctc ttctagatac
      481 gttgctatag atcccatact gagcaataat gggatcaata tcccaagaca aacctgaata
      541 ggtagatgat tcgttggaaa atggtttagt agctgaccat actgcaccat agcaaagcct
      601 aataaaaaca tcaacaccaa aacaaagtga tcatttaaga cgtaacgaag gtatttgttt
      661 tgttgttttt gaaagtcttg acggcgtttc agaaataatg ctttcatact taatcctctt
      721 ttgttaatgc aagatagata tcatttaagc tcgctgaacg gtcaccaaaa gcttcttgca
      781 agtcagcaag agttccttgg gcacgaactt gtccatgatg caaaatcaca aaacgatcac
      841 acattctttc agcagagtcc aaaacatgtg tactcatcaa aatagatttt ccttttgctt
      901 tttcgacttc tagcgtttgg attaagtctg aaatagctaa agggtctaac cctaagaatg
      961 gttcatcaag aataaataga cttggatcaa tgacaaaggc acaaataatc atgacttttt
     1021 gtttcatccc tttagaaaaa ttgacaggaa accattcaag tttgtcagtt aaacgaaaca
     1081 tttccaagaa aggttgtgct cgtttttgtg ccacttctaa gtcgatatca taagccatag
     1141 ctacggtgtt gatatgctct gataaggtca attcttcata gagactagga gtttctggga
     1201 taaaacctat tttttgacgg taggcaactg cattttctgc tagagttaaa ccatcaatag
     1261 aaatacttcc ttgataaggc ttcaaaaagc caataatttc gttgatagtt gttgactttc
     1321 cagcaccatt tagcccgatc aaaccgacta actctccatt gtctacagaa aatgagacat
     1381 cgtttaaaac agggatattg tgatatcctc ctgttagatt tttaatgttt aacatataat
     1441 ttctttctag tttttgatat acttataata tattataaca aattcaagta gagaaggtga
     1501 tataaaatgg aaaattgtat tttttgcagc atcattcaag gggatatccc ctcttcgaaa
     1561 gtctacgaag atgagcaggt cctcgccttt ttagatatct cacaaactac aaaagggcat
     1621 actctagtca ttcctaagca acatgttcgt aacttactag agatgactgc tgagacagct
     1681 agtcatctct ttgctcgtat tcctaaaatt gctcgtgcta tccagtcagc tactggcgct
     1741 actgccatga atatcattaa caataacgaa gccttagcgg gacaaactgt tttccatgct
     1801 catgttcatc tcgttcctcg ttataatgaa gaagacggta tctcaattca atacaccact
     1861 catgagcctg actttcctgt cttggaaaag ctcgcccgac aaatcaatca ggaggttagt
     1921 tcatgaaagt tgaaaccgtg atagccgtcg gattattatc ttttaccgct tacaaagcat
     1981 atcaaaaacg ttgtaccata aaagagctgt tatccatcag tagacaagca aaagatgctg
     2041 cccaacttga tttagataac attaaagcaa accttgacct cattcacagt caaggaaaag
     2101 taattcaaaa tattagtcag gacttggccc ataaatggcg ctactttaac caagagacac
     2161 aagcacacct cactgaaatc caaaatcgta tggccaaata tcaagaagat agtgagctaa
     2221 tttctagtga atcacctact tgccaaaccg tcattttaac taacaaccat tccagatagt
     2281 attatctccc tcatatactt tatttgaaat cacagacagc tatcatcttt gatacctgtc
     2341 ttttttatat tcaaaatcgg atattttaaa cataaaaacc ctcagaacgt taggtctgag
     2401 ggttgatatt agcaggagga actaatgatc cagaccctga tgagctagac tcatcagctg
     2461 ctaaagcatc tgatgaagaa gacggcgtag ttggcgtatg gcttgaagca gtagagtcct
     2521 ggtctgtact agcactctga taagatgagc cagtatctac tccacttgaa taattagcgt
     2581 aagagctatg actatcagaa taagatggag cctggcctga agagtcatag ttgttgttta
     2641 ctgtctgaga cttagttgac ccatacaaat tttcataaac agtagcattt gtttttaatt
     2701 gactaacctt atgaagtcct aattctgttc ggatacgatt ttggatttct aacaaatgat
     2761 tagaggtaac aatttggtat gatccaccat ctgataaagt ggcatcttct ccttttagtt
     2821 gataagtctt aatagttcta agtgcgtcac gataacctaa tagactaggg atagtgcgag
     2881 aagagatttc gatattcgtt tgcatattac tacttacagc agataaaatc ttccgataag
     2941 agctaatgct atcaagagca aggatttttt tcaatacctt ttgaatgact tcacgttgac
     3001 gcttttgtcg accataatct ccctcaggat catcataacg catacgagca taaaccaaag
     3061 cttgttcacc gttaattttg tgtgttccag gcgcaacagt agcttgatat tcaggttcgt
     3121 tttcagcaat cgagatagga aaatcaaact catttgtaac tgtaatccct ccaactgcat
     3181 tcacaagatc aataaggcct tgcatattaa tttgaacata gttatcaatg gtgatattca
     3241 aaagatcttg cacggtcata atagccatct gagcgccacc tgctgcataa gcagcgttaa
     3301 gcttagcttc aacaccattc atttcattat ttttgggtcc agataacgtg gttaaggtat
     3361 ctcgttctaa actagtcata gttgttttct tggtctttgg attaaccgta accaaaatca
     3421 tcgaatcact gtttccttcc cacttggagg cacgctctga agagcctgta tcaacaccca
     3481 tcaacaagat agaaaaagct cttgtttgtt taatggcatc acttttcttt gaagatgtcg
     3541 aaaaatcttt aaaggtcttt gataattctc ctgttgagaa ggtataagca ctagttagat
     3601 agacacctaa tgccaagaca gttgttaaca caatagctgt gaacattaaa actatttttt
     3661 ttccaatttt catcgattca cttactgact tacagattta cgcatatgat aaatatctaa
     3721 gtcatctcca ttttttgatt tgatgtcgtt ttttcgcata ctttcaatac gaaaaccata
     3781 ctttttatac aggtaaatag ccttagtatt tcttacttga acgtccaact taaggctttc
     3841 gatataaggg ttttcttcag cccaatcaag agcaatctct aacaataatt gaccgatacc
     3901 ataaccacgg taagtcttag caactaacat aaatacatct gcttcaactt ggccttggga
     3961 taatacttcc ccagaaagat ttaaaagccc tactactttt tcatctaatc tagctagcag
     4021 gcaaaaatca ataaaagagg tttgacttct tgataaaaag tctgacaatt cttgaggagt
     4081 agcctctaat atagtctcta cgcctgttat aaaatctgtt tctttggcag ctgccgtcac
     4141 taaggaaagt acagcttttg catcttgagg tacgccttct tcaataacga gactttgctg
     4201 aatcatgtga cattatctcc agaagctgac gagagcgctc accatgcgct aacaagtcta
     4261 gttgacgacc tttgtctcgt ttagtaatgg taatttgcaa atagtcttgt aataatccct
     4321 ctcctaataa ttctccccac tcaataacgg taacaccatt accaaaaaga aaatcatcta
     4381 aatcaataga gtctggatca tcaccaacac ggtaaatatc taagtgataa agaggtagac
     4441 gtccttcata ttctctaaca atagtataag ttgggctttt gatcatttga ctaatcccca
     4501 tccctttagc aatacctttt gccaatgttg ttttaccggc acctaagtcg ccagacaata
     4561 caatcacatc tccaatagac aaataagtac ctagggtttc tccgtaagct ttaagagtat
     4621 attcattttc actataaaac atagcaacat tatatcagaa aaaaaacata cttccaagca
     4681 ctacctagaa gatgactcaa ataaggtatt cgtaaagtaa aaacgcttct ttatattaaa
     4741 ggttaaaatc aattaaagaa gcggcagtta gtagcaaaat aagtaaatta tttggttata
     4801 agatagttaa tatgatgaag tttacaatga acaagaaggt tgctccccaa ataatagggt
     4861 gaatatcttt tgtttttccc tcaacaactt ttactagaca ataaaaaata aaggcagcgg
     4921 caataccata ggaaatagag caacacagcg ccataaagaa agctgcaaaa aaagctggaa
     4981 gagcatctgc aaatttactc caattaacat caagaaaaga agacaccatc atgacaccca
     5041 caacaattaa agctggagcc gtagcagcag ctgggacaat acctacaagc ggtaatagca
     5101 atattgataa taagaagcat actgcggtgg agactgctgt tagtccagta cgtccacctt
     5161 cagcaattcc tgctgctgat tcaacatagg tagtcgtatt tgaagttcca accaaagccc
     5221 caatagaagt accgatagca tctgcaaaaa gcgcacgatc catttttgaa ctaaagccta
     5281 tactattttc caaagcattc tcatcgtctt gagagaaaat acctgttcga cgaccagttc
     5341 cgataaaggt accaattgtg tcaaaagtat ctgatagact aaaagcaaaa atagtcatga
     5401 aaactagcgg taaacgactt gaatcgctaa aaagagaact caaaccatcg aaagctgcaa
     5461 ggaaagttgt acctaattca gtccaagctt caccaatatg gctgatccca aaattgaggt
     5521 tggacacatc cactattcct aaaggaatac ctacaagagt tgttgcgaca ataccaatta
     5581 aaatagcacc tctaaaattt cgaatcacaa gagctgtcgt taaaagtaaa ccaaaaatag
     5641 caagtaatac actagaatcc gtaaaactag aaatcgtagg tacaactcca ccattggcgt
     5701 ctacaaataa tagaccatct gcaaatgttt tagccgatgc tttagccggt tcaacaccat
     5761 ttaccattac tatattttca gcagagatag aaaaagtaat gatatttgcg tttttaaatc
     5821 ctaaataagc tacaaagaca ccaattcccc caccaatagc atgctgtaaa ctaactggaa
     5881 tcgccttgat gatactttta cgaaacttgg ttacggtaat aaaaatattg aataatccac
     5941 aaatgaaaac cattgccaag gcttcttgcc atgaaaaccc taaagcaaaa acaactgtat
     6001 aagtgaaaaa agcgttaagt cccattcctg gtgccaacgc ataaggcaca ttggcaaata
     6061 gtcccataat taaggttgat atggctgctg cgataatcgt agccaaaaag acagcattag
     6121 agggcatccc cgctgcacct aaaatacttg ggttaacaaa caaaatatag gacatggcaa
     6181 aaaaggtcgt taaaccagcc ataatctcag ttgagacagt tgtcccattt tcgcttaact
     6241 taaaaaactt ttccattgaa atggagtctc cttaattaaa tagtaggtaa aatcaaaaag
     6301 cgcttatcac tagataagtc tttttgacac ctcattatta ttgttattgt tcgtattaaa
     6361 atgaacaata acaatatgtt accatttttt tgttcacttt tcaacgtcta taacttcatt
     6421 tattcattaa aaaaacagtt atcctatctt ttttactaat gaatacactt aacttatctt
     6481 ttctctttat cttggagcaa aatttgctat aatttaatta tgactaaaaa attgattgcc
     6541 attgatttgg atggaacctt attacatcat gacaacacca tctctaccta tactcaaaaa
     6601 accatcaaag ctgtccaaga caagggccac cacgtcatta tttcaacagg ccgtccttac
     6661 cgcatggcac tggactacta tctgcagctc aatttaaaga ccccaatcat tacttttaat
     6721 ggggccttga cccacatgcc tgaacaaaaa tgggcctatg aacataatgt cactttggac
     6781 aaaggctacc tactaaggtt actacaatat caggatgatt tccagatgga ttttattgcc
     6841 agtgagtatc gaaaaaatgt ctacattacc atgaccaatc cagaaagtat cgacccgcaa
     6901 ctctttggtg ttgatgaaat tactcaggac atggcattag agattaccaa aatcactcga
     6961 aatccaaacg ccttattgat gcaaacgcgc cacgaagaca agtatgcctt agctaaaaac
     7021 atgcgtgctt tcttcaaaga tgaaattgaa attgattctt ggggaggccc tctcaacatt
     7081 cttgaaattt cgtctaagaa tgtcaataaa gcttacgctc tgaactatct ccttggaatt
     7141 tacaatatgg ataaaaagga tttaatcgcc tttggtgatg aacacaatga cactgagatg
     7201 ctagcttttg ctggaactgg ttatgccatg aaaaatgcta gccctgtgct actgccttat
     7261 gctgatcagc agcttaattt ttccaacgaa gaagacggtg ttgccaaaaa attggaagaa
     7321 cttttcttgt agtagcaaac gaaaccacta tttttcagaa aatggt
//
LOCUS       DAUELZ010000082         7123 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.083, whole genome shotgun sequence.
ACCESSION   DAUELZ010000082 DAUELZ010000000
VERSION     DAUELZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.083"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            17..454
                     /gene="argR"
                     /locus_tag="VHI09_001335"
     CDS             17..454
                     /gene="argR"
                     /locus_tag="VHI09_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ6121211.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            451..803
                     /locus_tag="VHI09_001336"
                     /pseudo
     CDS             451..803
                     /locus_tag="VHI09_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
     gene            790..3345
                     /gene="mutS"
                     /locus_tag="VHI09_001337"
     CDS             790..3345
                     /gene="mutS"
                     /locus_tag="VHI09_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEQ6121212.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAIDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFNSAYVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRNGFDERLDHYRKVMREGTGWIADIEAKERQASGINNL
                     KIDYNKKDGYYFHVTNSNLSLVPDHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKILATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIMSVPP
                     QVESSSAVRQGQLSLFGDEEKAHEIRQALEAIDVMNMTPLQAMTTLYELKKLL"
     gene            complement(3741..4394)
                     /locus_tag="VHI09_001338"
     CDS             complement(3741..4394)
                     /locus_tag="VHI09_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121213.1"
                     /translation="MWSSIFNFKENIVKKKLVMMLTLLISICAISVRANESGDLFTTI
                     GQSHLPGVTVAPFKTGDTTINVNLDPYGYVYGFVTSSNGNKIKKTENKTYEIPCSILE
                     KQDNPNCEGRKWTVSGNRADGGGNYTVQLEKPLAEGDKVTLSFADDGNLYFGQLVFED
                     GKTHNERLQKERDAEEQQYAEVLFKRSIEEESKKTWHQRLSDSIQDQWWNFKDWLRG"
     gene            complement(4559..4942)
                     /locus_tag="VHI09_001339"
     CDS             complement(4559..4942)
                     /locus_tag="VHI09_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEQ6121214.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLTYTELYQFDIEYHPTRRKH
                     LEISRECALEELDAIRYAINQLSKVEYRQILIECYLISEEKTQQDIMEELNRSQSWYY
                     ESKKRALLEFVEFYRDGALKNNVRL"
     gene            complement(4917..5126)
                     /locus_tag="VHI09_001340"
                     /pseudo
     CDS             complement(4917..5126)
                     /locus_tag="VHI09_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
     gene            complement(5178..5378)
                     /locus_tag="VHI09_001341"
     CDS             complement(5178..5378)
                     /locus_tag="VHI09_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121215.1"
                     /translation="MNLCQYLGKDIKVTFVDGQVLEGHCNTFTGKPDTEDELYDEITI
                     KTDKNPYIGFNESEIKSIEIVK"
     gene            complement(5453..5866)
                     /locus_tag="VHI09_001342"
     CDS             complement(5453..5866)
                     /locus_tag="VHI09_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEQ6121216.1"
                     /translation="MLPEQIKSKLERIKHLDIEIKGLYYELQYIDDSLFQKSTLTPSK
                     VKTSQDNGIEKHLLNQLELKDKITESIHKLIQERLKITELINLLDAPLERTVLRMYYV
                     NHLEVWKIAEQLSKGEATVYRKLREAIKHLSEVVQ"
     gene            complement(6058..6294)
                     /locus_tag="VHI09_001343"
                     /pseudo
     CDS             complement(6058..6294)
                     /locus_tag="VHI09_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
     gene            complement(6321..6935)
                     /locus_tag="VHI09_001344"
     CDS             complement(6321..6935)
                     /locus_tag="VHI09_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEQ6121217.1"
                     /translation="MRTETWNGYTIRFVEHQGEWWAVAKDITNALGLKQPSRAISTLK
                     GVTKSKTLTKGGEQELSIINEKDIYRLVFKSRKPEAEAFQEWVFETIKQLRQSIGLEG
                     FQVFRMFDKEHQKQAMNRLVNCLQNATKKDLIKANTIANKAVSDLYGYPKMVSKNEMT
                     ENMLRDREPILDETVELIKVKEKYGLNFSVSEAIYNQNTIKKAQ"
     gene            complement(6949..>7123)
                     /locus_tag="VHI09_001345"
     CDS             complement(6949..>7123)
                     /locus_tag="VHI09_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6121218.1"
                     /translation="LAKRRKKVSDVAKSTGLSSGTLISLYYERTKRPDIETLQKVADY
                     LEVTLDELLTVED"
BASE COUNT         2076 a         1328 c         1348 g         2371 t
ORIGIN      
        1 attggggtga tatatcatga ataaaatgga gcgtcaacag cagattaagc gaatcattca
       61 agcagagcat attggcacac aagaagatat taaaaatcac cttcaaaaag aaggcattgt
      121 ggtcacccaa gcaactctat ctcgggactt aagagaaatt ggccttttga aattgagaga
      181 tgaacaaggc aaactctatt atagtttgtc tgaacccgtt gcaacaccat ttagtcctga
      241 ggtgcgtttt tatgttttga aagttgatcg agcaggtttt atgctggttc tccataccaa
      301 tttaggagag gctgatgttc tagctaattt gattgacaat gatgccattg aagatattct
      361 aggaaccatt gctggtgcgg ataccttgct tgtgatttgc agagacgaag agattgctaa
      421 acgctttgaa aaagatttag ctgcaggcct atgactagct atcaagagtc tttgacacaa
      481 ttgattcttg ctatccatga attctagtat cgttgcttat aaagaagtgc agcaatagct
      541 gtctaagctc cgtgatttta aaaagtaagc ttatcagatg aaacgctatc aacaagatgc
      601 cctgcttttc aaaaaaaata gataaagaaa aggctgcgac agtatctgca agcaggacaa
      661 aagaactaga agataggctc agtcatcagc cattaattga tgattatcga gaaaagatgc
      721 aagatgcaag tgatgtgatt cagtatatca ccaaacgtat agaagatcag ttaaacaagg
      781 agttaacaaa tggcaaaaac taacatttct cctggaatgc aacagtatct ggacatcaaa
      841 aaagattatc cagatgcttt tttgcttttt aggatgggtg acttttatga attattttac
      901 gaggacgctg tcaaagcagc acaactctta gaaattggtt tgaccagtcg caacaagaat
      961 gcggaaaatc caattcccat ggcaggcgtg ccacatcatt ctgcccaaca atacattgat
     1021 gtgttaattg agttgggtta caaggttgct gtcgcagaac aaatggaaga cccaaagcaa
     1081 gctgttgggg tggtgaagcg tgaggtcgtt caagtcataa ctcctggaac ggttgtggat
     1141 tcagctaagc cagatagcgc caataacttt ttggtagcta ttgactttga tggttgccgt
     1201 tatggattgg cttatatgga tgtgtccaca ggtgaatttt gcgtgacaga tttggcggac
     1261 tttacgagtg ttcgtagcga aatccaaaac ctcaaggcaa aagaagtctt actaggtttt
     1321 gatttatctg aagaagaaca gacgattttg gtcaagcaga tgaatttgct gctttcttat
     1381 gaagaaacgg tctatgaaga taaatcttta attgacggtc aattgacaac ggtagaactg
     1441 acagcggcag gaaaactctt gcaatacgtt cacaaaacac aaatgcgaga actcagccac
     1501 ttgcaagcct tggttcacta tgaaatcaag gattatttgc agatgtcgta tgccactaag
     1561 tcaagtctag atttggtaga gaatgctaga acgaataaaa aacatgggag tctgtattgg
     1621 ctgttagatg aaaccaagac agctatgggg atgaggcttt tgcgctcatg gattgatcga
     1681 cctttggttt ctaaagaagc tattttagag cgtcaagaaa ttattcaagt ttttctgaat
     1741 gcttttattg agcgaacaga tttaagcaat agcttaaaag gtgtttacga tatcgaacgc
     1801 ttatctagcc gtgtgtcttt tggcaaggca aatccgaaag atttacttca attggggcat
     1861 accttagccc aagtgcctta tatcaaagcc atattagagt cttttaacag cgcttatgtt
     1921 gacaaacttg tcaatgatat tgacagtttg cctgagttgg aatacttgat tagaacagcc
     1981 attgatccag atgcaccagc aactattagt gaaggaagta ttatccgcaa tggttttgat
     2041 gagcgcttgg accattatcg taaagtaatg cgagagggga caggctggat tgcggatatt
     2101 gaggccaaag agcgtcaagc aagtggcatt aataacctaa aaattgatta caataaaaaa
     2161 gatggttatt attttcacgt tacgaattca aatcttagct tagttcccga ccattttttc
     2221 agaaaggcaa ctttaaaaaa ttctgagcgt tatggaacag cagaattggc taagattgaa
     2281 ggtcagatgt tagaggctag ggaagagtca tctagtttag aatacgatat ttttatgtgt
     2341 attcgagctc aagttgaaac ctatattaat cgtttacaga aactggctaa aattttggca
     2401 acggtagatg ttttgcaaag tttagcagtc gttgctgaaa ccaatcatta tatccggccg
     2461 cagttcaatg ataatcatgt gattacaatt caagaaggtc gtcacgcggt tgttgaaaag
     2521 gttatgggag tgcaggaata cattcccaat agtatctctt ttgaccaaca gaccagtatt
     2581 cagctgatta caggtccaaa tatgagtggt aagtcgactt atatgagaca gctggcctta
     2641 acggttatca tggcccagat gggttcattt gtggctgctg accatgttga tttaccttta
     2701 tttgatgcga tttttacgcg tattggggct gctgatgatt tgatttctgg gcaatcaacc
     2761 tttatggtgg agatgatgga agcaaaccaa gcaatcaaac gcgcaagtga caactctctt
     2821 attctatttg atgaactggg acgaggtacg gcaacttatg atggtatggc tttagcccag
     2881 gcaattattg aatatatcca tgatagagtt ggtgctaaga ccatatttgc aacgcattat
     2941 catgaattga cagacttgtc aactaagttg acaagtctag tcaatgttca tgtagcaacg
     3001 cttgaaaaag atggcgatgt taccttcctt cataagattg ctgagggacc ggcggataaa
     3061 tcttacggta ttcatgttgc aaaaatagca ggactgccaa aatccctatt aaagagagca
     3121 gacgaagttc tgacccgttt agaaacacag tcacgatcta ctgagataat gtcagtccct
     3181 ccacaagttg agtcaagcag cgctgttaga caggggcaat tatccctttt tggtgatgaa
     3241 gagaaagctc atgagattag gcaagcactg gaagctattg atgtcatgaa tatgaccccg
     3301 cttcaagcaa tgacaaccct ttacgaattg aaaaagttgt tatagttttt cagctgaaaa
     3361 tgaaaaagat gctttctata ttgaaggcat ctttttgttc tgtcaaaaac ggtccgaggc
     3421 cttcggctat ttattaagtg tgttataata gtccataaga atgcgaggaa attatgacaa
     3481 acattattgg acttggattg gcagtcttta cagggtattg acagtcctta agcattgtta
     3541 tgactgtgtt tttgaaaaaa tataggtttt aagtttcctt agacttccct caaaagtcca
     3601 caaaaaggtg aacaaaaaag acctttacag gtcctttaca caatgagttc agcaggcaag
     3661 aactagcgtg gtttaaatac tacgcttttt agtttgccct atggcttatt atagcacgat
     3721 tagtgtcatt gaggaaaaaa ctaccctctc aaccagtctt taaagttcca ccattggtct
     3781 tggatgctat cgctcaagcg ttgatgccaa gtttttttgg attcttcttc gatagaacgc
     3841 ttgaataaca cctccgcata ttgttgctct tcggcatctc tctctttttg aagacgttca
     3901 ttatgtgttt ttccatcctc aaatacaagt tgcccaaagt atagattccc gtcatccgca
     3961 aaagataagg taactttgtc tccttcagca agtggttttt ccaattgaac tgtgtagttg
     4021 cctcctccat cagcacgatt gccacttact gtccatttcc ttccttcgca gtttggatta
     4081 tcttgtttct ccaaaataga gcaaggaatt tcataagttt tgttttcggt ttttttaatt
     4141 ttattaccat tacttgaagt tacaaaacca taaacatacc catagggatc tagattaaca
     4201 ttaatggttg tatctcctgt tttaaatggt gctacggtta ctccaggtaa gtgcgattgc
     4261 cctatagttg taaacaaatc gccactttca ttagctctga cacttatagc acatattgat
     4321 attaacaaag ttaacatcat aactaatttc tttttcacta tattctcctt aaaattaaat
     4381 atcgaactcc acaataccag ttaatcagtc aaaaaatcca ttctactttt tgatatttta
     4441 tgtctaattt gacccgctaa gtcagatttg caaacgatac caagtgattt tcttctaatc
     4501 attatttttt agatggatat gttaattgta ttagagacgt attaatctta aaccattgtc
     4561 ataagcgaac attatttttt agcgccccgt ctctgtaaaa ttccacaaac tcaagcaagg
     4621 ctcttttctt agattcataa taccagcttt gacttctgtt aagttcttcc ataatgtctt
     4681 gttgagtttt ttcctcactg atcaagtaac actcaattag tatttgtcta tattctactt
     4741 tagacagttg attaatggca tacctaatag cgtctagttc ctctagggcg cattctcggc
     4801 ttatttcaag gtgttttctg cgcgtgggat ggtactctat atcaaactgg taaagctcgg
     4861 tataagttaa atcaaggcta ttagcgatac gttgccatct atggaattct ttcagcttac
     4921 gaatagcgtt cttcttgctc atctaacacc tctaaagctt ctctatgtag tttgaacaca
     4981 gtgtttcttg agtaacctag tttatcgagt atctcgtccc atgataagtc atccacgtat
     5041 ctagccttaa taacagctat ctgtctttca tgttgcaatg tggcaatcat ggccagtctc
     5101 ttgtcacgtt cctagctaca atatactcct ttgggagggg acataataaa aagtaagtag
     5161 ctatgatagt caagtgctta ttttacaatt tcaattgatt tgatttctga ttcattaaaa
     5221 ccaatatatg ggttcttatc agtttttatt gtgatttcat catacaactc atcttccgta
     5281 tcaggttttc ctgtgaatgt attacaatga ccttcaagga cttgaccatc aacaaaagta
     5341 actttgatat cttttcctaa atactggcat aaattcatta ttggtctcct ttcttaggta
     5401 ctgtatgggt tctactttta taatattagt tcctatagtt caaatctcac ttttattgaa
     5461 ctacctcaga taagtgtttt atagcctcac gaagctttct gtagactgtg gcctctcctt
     5521 tactgagttg ttcagctatt ttccaaactt ctaagtgatt aacataatac attcttaaaa
     5581 ctgttctttc aagaggagcg tctaacaaat taattaattc agtgattttt aatctttcct
     5641 ggatcaattt atggatagat tcagtgattt tatctttgag ttctaattga ttaagtagat
     5701 gtttctctat accgttgtct tgacttgttt ttacttttga cggagttagt gtcgatttct
     5761 gaaaaagact atcatctata tactgaagtt cgtaataaag acctttaatc tcaatgtcta
     5821 aatgtttgat acgttccaat ttagatttta tttgctcagg taacattcta accctccaat
     5881 tatgatatac tataatcaaa tctgatatta ttcaaggatc ttccttgtac ccaagagtcc
     5941 tttccctaca aagttttgta ggattttttt gttattctaa cgtttttagg acactttcaa
     6001 caaaatcttc atcatgaact tcaataccgt taacgatatg aattccagta aatccccatt
     6061 gccttgttca atgtcattca aacgcttttg ctctgctttg cgattataga ttagcacttt
     6121 gtcatcaagc atgagcgata cgccttccaa tacgttgaaa atttcctgtg tgattgcttc
     6181 aaactgttcg cgatctgcat ttggtacttt gtcagcgtaa ccctgtgcta gctcagctaa
     6241 atcaacacct tcatcaatcc atttcttcaa gtctttgtaa gttgttgctt tcataatcat
     6301 ttctccttta tccacgcgca tcactgcgct ttttttattg tgttttggtt atagatagct
     6361 tcagatacgc taaaatttaa gccgtatttt tctttaacct tgattagttc aaccgtttca
     6421 tcaaggatag gctcacggtc tcgcaacatg ttttctgtca tctcgttttt actaaccatc
     6481 ttaggataac catataggtc agaaacggct ttgtttgcga tagtgtttgc tttgataagg
     6541 tctttcttag ttgcattttg taagcaattg acaagcctat tcattgcctg cttctgatgt
     6601 tctttatcaa acattctaaa tacttggaag ccctctaggc ctatgctttg tcttaactgt
     6661 ttaatggttt caaataccca ttcttggaaa gcttccgcct caggttttcg acttttgaat
     6721 actaatctgt aaatatcttt ttcattgata atagaaagct cttgctcccc accttttgta
     6781 agggtcttac ttttagtaac cccctttaga gtcgatatag ctcttgacgg ttgttttaat
     6841 cccaaagcat ttgtaatatc cttggcaaca gcccaccatt cgccttggtg ctctacaaat
     6901 cggatagtat atccgttcca tgtttctgtt ctcaataagt tttcctttct agtcttcaac
     6961 tgttaataat tcatcaagtg taacttctaa atagtcagca accttttgaa gtgtttcaat
     7021 gtctgggcgc tttgttcgct cgtaatataa agatattaaa gtgccacttg ataacccagt
     7081 tgattttgct acgtctgaaa cttttttgcg tctttttgct aaa
//
LOCUS       DAUELZ010000083         7027 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.084, whole genome shotgun sequence.
ACCESSION   DAUELZ010000083 DAUELZ010000000
VERSION     DAUELZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.084"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            31..702
                     /gene="sdaAB"
                     /locus_tag="VHI09_001346"
     CDS             31..702
                     /gene="sdaAB"
                     /locus_tag="VHI09_001346"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEQ6121219.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGVSIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
     gene            714..1586
                     /gene="sdaAA"
                     /locus_tag="VHI09_001347"
     CDS             714..1586
                     /gene="sdaAA"
                     /locus_tag="VHI09_001347"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEQ6121220.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKKQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIESQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(2000..2614)
                     /locus_tag="VHI09_001348"
     CDS             complement(2000..2614)
                     /locus_tag="VHI09_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEQ6121221.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNDDTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKEKAKSIASEPIPTVENSVAPTVTEEAPVVQQEETQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIAHESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
     gene            complement(2985..3785)
                     /locus_tag="VHI09_001349"
     CDS             complement(2985..3785)
                     /locus_tag="VHI09_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ6121222.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
     gene            complement(3778..4620)
                     /locus_tag="VHI09_001350"
     CDS             complement(3778..4620)
                     /locus_tag="VHI09_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ6121223.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELREV
                     LKHG"
     gene            complement(4596..5435)
                     /locus_tag="VHI09_001351"
     CDS             complement(4596..5435)
                     /locus_tag="VHI09_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ6121224.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPIDYGYLTEKELENQLCQLI
                     SKM"
     gene            complement(5437..5979)
                     /gene="pgsA"
                     /locus_tag="VHI09_001352"
     CDS             complement(5437..5979)
                     /gene="pgsA"
                     /locus_tag="VHI09_001352"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEQ6121225.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVVSNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMLGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            complement(5993..7018)
                     /locus_tag="VHI09_001353"
     CDS             complement(5993..7018)
                     /locus_tag="VHI09_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ6121226.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSKFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIVILGTIAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTNGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
BASE COUNT         2273 a         1333 c         1180 g         2241 t
ORIGIN      
        1 taaacataga aataaaaaac ggagttgcct atgaatacac aaaaatttca atcagttttt
       61 gacatcattg ggccagtaat gattggtcca tcaagtagtc acacagctgg tgccgtcaga
      121 attggtaagg tagtccattc catctttgga gatattcctg atgaagtgac gtttcacctt
      181 tataattcat ttgctaaaac ctacagagga catggtactg ataaggctct tgttgcagga
      241 attatgggta tggatactga taatcctgat attaaaaatt ctctagaaat tgctcatcaa
      301 aaagggataa aaatctattg ggatatctta aaagatagta atgctcccca tcccaatact
      361 gttaaaatca gtgtcaaaaa agctgacaaa acacttagtg ttactggtgt ttcaattgga
      421 ggaggaaaca ttcaagtaac agaactaaat ggtttttctg tctcgttatc catgaatact
      481 cctactatag ttactgtaca caaagatatt cctggaatga tcgcaaaggt aactgatatc
      541 ctatcttcca ataatattaa tatcgccaca atgaatgtta cacgtgaatc cgctggtgaa
      601 aaagctacta tgattataga agtcgattct agagagtgcc aagaagcagc caaccaaatt
      661 gcaaaaattc ctcatattta taatgtcaac ttctttgact agaaaggaaa gctatgtttt
      721 atactattga agaacttgtc aaacaagctg accaacaatt taatggaaat attgctgaac
      781 tcatgattgc cacggaagtg gagatgtctg gaagaaatcg agaagatatt atcaaaatca
      841 tgtcacgcaa tttacaagtt atgaaagcag ctgtaacaga aggactaacc tcaaccaaat
      901 ctatcagtgg tttgacaggg ggcgatgcgg tcaaaatgga taactacatt aaaaaaggga
      961 acagcctttc tgatactact attcttaatg ctgttagaaa tgctatagct gtaaacgaat
     1021 taaatgctaa aatgggactt gtctgtgcaa cgcccactgc aggaagtgca ggatgcttac
     1081 cagcagtgct cgcaacagct attgaaaaac ttgacttatc agaaaaaaaa caattagagt
     1141 ttttatttac tgcaggagct tttggtcttg ttattggaaa taacgcctct atttctggtg
     1201 cagaaggtgg atgccaagca gaagttggtt ctgcagctgc tatgagtgca gctgctcttg
     1261 ttaaggcagc tggtggaaca tctcatcaag ctagtcaagc tattgcgttt gttatcaaaa
     1321 atttattagg acttgtctgt gatcctgttg ccggcctagt agaagtgcct tgtgtcaaac
     1381 gcaatgccct aggagcaagt ttcgcccttg tcgcagctga tatggcttta gctgatattg
     1441 aatctcaaat tcctgttgac gaagttattg atgctatgta tcaagtcggc tctgccatgc
     1501 caactgcttt ccgtgaaaca gcagaaggtg gtttagccgc tactccaact ggacgacgtt
     1561 acagcgtaga aatatttggc gaataaatag atagttagaa aaattcttag aaatatttga
     1621 aagcgtttta tttttctgct atatattcac taaaatgact aatagatagc tatcaagcta
     1681 tgttgtttcc ccttagaaac aactcctagt aaattgttga aactcaaaag tgtagtagtg
     1741 taaaatactg aaaattgcta atgaccttat aaacatttaa gagttttttt cctgcttgtt
     1801 atcagtataa ctgccttaaa tatcagaggc acttaagtgc tggtcagttt tcctaaataa
     1861 tacgagctat attccctgat aaggcaatgt tttgttttga gactaattca tcactacaaa
     1921 tcataaagac aattaaatac caaaaaccta ttgttagtaa atactaacaa taggttttat
     1981 ttcttccagc tatgtttatt tagtaacccc aagctgataa accttgtgca ctataggctt
     2041 tcaaggctgc attgacttga tcttcaaccg ttgctgtaga accccaacct ggcattgtct
     2101 ggaacaaccc tgatgcccca gaagcattag ctgcgttagg atttccatta gattcatgcg
     2161 caattatatg ttcccaagtt gattgtggaa cacctgttgc tgctgccatc tgtgccgccg
     2221 cttgacttcc tacaatacca gcagtatttc cattagaaag taccacattg tttggattat
     2281 aggctactga agatacctgc tgaacagttt gagtctcttc ttgctgaaca accggtgctt
     2341 cctctgttac tgtcggagct acagagtttt ctactgtggg aataggctcc gaagcaatgc
     2401 tctttgcttt ttctttgctt tcttctttag taatagcatg attatttttt ggagccttgt
     2461 cgattgttac cattttactt tctgacttct tagcataaga cttagtatca tcatttttac
     2521 ttgagaaggc aaaaatgatg gctaatattg ttagagctaa cgctactaat ccaaaattaa
     2581 aataacgttg ttttaaattt tctttcttaa acattcaata atacctcact tttctccgtt
     2641 atccataata tcttttaatt attacctcgt tattgaagat atattaaaaa tattacaact
     2701 acattgatat tataagaagt tgtaaaaaat cataaaactc ttcttcttag cgatattaaa
     2761 aagcgggata atatcattaa actaaggatt tagttctgta ttgaacagga ctcagtcctt
     2821 ttagatttcg ttcgatttgt tattgttggg atgattaata tagttgagat agcggttttc
     2881 aattcctcaa atgaagaaaa gtacctcaat cattagatgt tgtatctaac ttttgaggta
     2941 caattcactt cttataataa agaaaagttt tgtgctaaaa aatattacaa agaggttttc
     3001 aagaaaaaaa gtagcaaacc caataaagaa actattccaa tagctaaact atcttttagc
     3061 tgccagtcta attgacgata tttagttcgc ccttctcctc cctgataacc tctagcttcc
     3121 atagcaatag ctaatgcgtc ggctctttta aagcttgaag cgaaaagagg gattaaaata
     3181 ggaataatgg atttaacttt ttgaattaaa tttccctctc caaaatcaac tccacgggcc
     3241 ctttgtgcat tcataatacg cgtggtatca tccattaaag taggcacaaa acgtaaactt
     3301 aaagaaagca ttagtccgat ctcgtgagct ggaactttaa atcttgttaa aggttttaat
     3361 aatgattcaa ctgcatcaga taggcttaaa ggtgttgtcg tcaacgttaa cagcgttgaa
     3421 aagaaaataa ttagaacgaa acgcataaaa attaaaatag cttggctcaa tcctaaatca
     3481 gttatgctaa tgaaccacca actaaaaatt acttttcctc cttgtgagaa aaacatctga
     3541 aataaagtcg tgaagaggat aataccaatc attggtttaa ccccatttaa aaagaaagac
     3601 aacttgattt ttgacaaaaa aacaactgct aaagtaaaag tcaacatcaa caaatttgtc
     3661 acaacattgt tagcccaaaa gataatgaca atataaataa tcatcgctaa taatttactt
     3721 cttgggtcaa gacgatgaat gagagaatct ccgggaatat aacgaccaag aatcagctta
     3781 tccatgtttt agtacctctc ttagctcctc taaggtaata ggcaaagaag agataggtat
     3841 tcctctatca acaagccttt gagcaagttt agtcacctta ggaactccta actgtttttt
     3901 ctcaagcaaa ctgacttgtt gaaaaattgt ttttggtttt cctgataaaa taattttacc
     3961 tttgtcaaga acatatacaa aatctgcgta attggccaca tcatccatca gatgggttac
     4021 taaaacaata gtcatgccag attggtgtaa ctttttaaaa atagtcatta attctttacg
     4081 ccctttagga tcaagaccag cagtcggttc atctaaaact aaaacctttg gctgcatagc
     4141 aaggataccc gcaatagcaa cacgacgcat ttgaccccct gagagttcaa atggattctt
     4201 ttcaaaaaga ttttctgaaa tcccaacaag agctagtttt tcacgcgcta aagcttctgc
     4261 ttcctccgga gagaccccaa aattttgagg gccaaaagca acatctttca aaactgtctc
     4321 ttcaaatagt tgactttctg gaaattgaaa aactaatcct acatgttttc gaatagattt
     4381 aatctcttta tttttagagt gattcgttat atcttgttta tctacgctga caattcccgt
     4441 tgtcggaacg tgtaaaccat ttagcaattg cataatagtc gatttcccgg atccagtatg
     4501 tccgataaaa gccgtataag aaccatctaa aatatctaaa ttaatgttaa aaagggcacg
     4561 cccttcaaaa ggagtccctg cttgataagt ataacttaca ttttggagat taattgacat
     4621 aactgatttt ctaattcctt ttctgtgaga tatccatagt caataggata accttcttct
     4681 tgaagcatct gtacaacaga tgttgtaaaa gggatatcaa gacctaattg gagtagttca
     4741 tcccctcttg caaataattg ttctggtgtt gatgttgatt ccacttgacc atctttcatc
     4801 actaaaactc tatcactaag agcaacttca tctaagtcat gagtaatgga aataacagtc
     4861 agctggtagt cgtcacggat gttttttata gttttaatta actctaatcg tcctttaggg
     4921 tcaagcatac tagtagcttc atctaaaata ataattttag gcttcatagc aactgcacct
     4981 gcaatagcta cgcgctgttt ttggccacca gataaacggg ctggttcttt ttctttaaag
     5041 ttctgcatgc cgactaactc taaagcatga ttaaccctct cttttatatc ttcatgtgca
     5101 atacctttat tttcaagccc aaaagcaaca tcatcttcaa cagttgctcc aacaaactga
     5161 ttatcagggt tttgaaagac cataccaatt ttatgacgaa tttcccaaac attagttatg
     5221 gttagtagat ctccatcaac aataatagac cctgattctg gttccaataa tccatcaatt
     5281 aaccgaatag tcgttgattt accagatccg ttatggccaa tgatagacag ccactcacct
     5341 tgtttcacat gaaacgatac gccatctaat gttggttttt cttggtcttt atggtaatta
     5401 aatgtaactt ttttaagttc aataatagct gacatgttat ttaaacgtat ccttaaaaag
     5461 aaagcttgct cctttaaaat aatcatatcc agaataaata gtgaaaaaaa gagcaatata
     5521 gagtaagacg tttcctagga atatccaatg gcaaagtaat aaaataatag acaacatttg
     5581 tgtcgctgtt ttgatttttc ccagcatagc agctgcaagg acctttcctc cggtttcaac
     5641 aagtagtaat cgaagaccag ttactgccaa ttctcggcaa ataataacag ctgataccca
     5701 cgcaggaact aagccaagtc caactaacat gataaaggca ctcatgacaa gcatcttatc
     5761 tgctagagga tcggcaaact ttccaaaatt actgactaca tgccacttac gcgcaagata
     5821 cccatctaga taatcggtaa aactggcaat tgcaaaaatc acagctgcaa aaatatgcca
     5881 ccccacctta ttagatgagg aagtaataaa aagaaagaaa ggaatcattg cgattcgaac
     5941 aagagttaat agattaggaa tattttcttt ttttatcatt agtctacctt aattattgga
     6001 tcttaaacgt gatataactt aagtcagtac ttgttaaagg tgatagatca acaggttgtc
     6061 catcaattgt tacagataca ccatttgtca caccaagcgt caacaatgat tctgttattt
     6121 ctgcaggtaa agtcgctgta taagttggcg agtcttgagt caatgttata ccaccttcac
     6181 caatttctga atttgttaga gaaatccaac tactttgtgc ctcggttaaa gacacactga
     6241 tatcaacggt ctctttagat ttagtaacag tcgctattaa ataattcccc gacccctcag
     6301 ttgtgacttg agttttgcga tcatttgaat tattttttaa accgttagta gactgctcag
     6361 aggctttaag caaagcaact tcagcttctt tagctctttg actcttttcg aattgttgcc
     6421 aaacacctaa aatgataaca ctgataattg caaaagcaat cgttcctaat ataactatag
     6481 gaaatagtga cgttttagaa cgactgtcag tgctatttcg cctcatacgt gacacatttt
     6541 ttttaggaat taacctgtta tctgatttaa atttgctaac aggcgatatc gtttttagtg
     6601 gcgatttgct atctaaacca acagtcttta cagggataac taaggtttct ttgtttttag
     6661 aaacttctaa cggttgacca taccccatgc tctgatgtga taacaacttc tcttgtaatc
     6721 gtttcttact taacttctct tcaacaatct cagttacaga ttttatatca ctgtcttttt
     6781 tggaattaac ttgataacgg tatcgtcttt ttaaagtatt gaaatctaac tcaacaatgt
     6841 cagcatattc ttttaaaaat tgatcaaatt tttcttccgg aataatttta aactgatcca
     6901 attccattgc cagcaagtaa tgggaagaaa tccctgtctt caattctata tcatctagtg
     6961 tgatattttt tccaacacgc gattctctta acacttcacc aagacttttt tcactcatac
     7021 aggtacc
//
LOCUS       DAUELZ010000084         6972 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.085, whole genome shotgun sequence.
ACCESSION   DAUELZ010000084 DAUELZ010000000
VERSION     DAUELZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.085"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            48..3263
                     /gene="rexB"
                     /locus_tag="VHI09_001354"
     CDS             48..3263
                     /gene="rexB"
                     /locus_tag="VHI09_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEQ6121227.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRTLMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVVVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFLKKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELINFGLPLLDKGKNYLSYDNSDIANYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIQVIDQE
                     KNFQLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLVTYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMGQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            3224..6892
                     /gene="addA"
                     /locus_tag="VHI09_001355"
     CDS             3224..6892
                     /gene="addA"
                     /locus_tag="VHI09_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEQ6121228.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERCSDQSQKRTAQ
                     QIEAIYTSGQNILVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLCQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQGVLKQEVFSKLFSEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKYTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTGLIPSGNYVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDIAHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGDVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYGIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNVFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQE
                     ITDPKKLDKLLPLALREQLLTFQDWLLAIADIFSTEDLYFDVRFIEDSDLTQESVGRL
                     QTPQLLNPDDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKAAYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKAKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALRGIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
BASE COUNT         2336 a         1194 c         1293 g         2149 t
ORIGIN      
        1 ttgctataat ataacaaggg tagattcagt ttgtataaag gagtcatatg aaattaattt
       61 acacagagat gagttactcg atgacagaga ttttggtgaa tgaagcacga aaagctgctg
      121 accaaggtta tcgagtgttc tatattgctc caaattctct ttcatttgaa aaggaaagag
      181 aagtcttaac cttattgcct gaacgaggga ctttttcgat aatagtcacc cggttcgttc
      241 aaatgtcaag gtattttaca gttgagagct ctccttctaa gcaacatcta gatgacacga
      301 ctttggctat gattttttat cggaccttga tgcaattaaa accagaagat ttgccgagtt
      361 atggtcgttt gcaaaataat tcggttttta ttgaacaact agttgagtta tataaggaat
      421 taaaaaacgc acaattaagc gtacatgatt taacagggtt agaccatcct caaaaacaag
      481 aagatcttat taaaattatt gagcttgcag agacgatcat gattcagcaa gattataatc
      541 aagatagtcc tctacaatct tttgcaagag caattaagtt aggactttta aataaccaac
      601 tatcaaaaac agttgttgtt attgatggtt tttcacgttt ttcagctgaa gaggattatt
      661 tattatcact tttaaataac aactgtcaag aggtcatcat tggcagttat gtgagtcaaa
      721 aagcttatca aaaatctttt attaagggaa atatctatga ggctagcctt cattttttgc
      781 aagatttggc tcaaaaatac catatcaagc cggtatttgc aacctctaat caagtgttta
      841 aaccagcctt ttcacggcta acacaattat ttgaagcaac tcatgatttt tcacaagtag
      901 actggcaact ccaaaagaat gatcttgatc actttagcct ttggcaatgt catcatcaaa
      961 aagaggaaat tgaacatgta gcaaaatcca ttcggcaaaa gctatatgaa ggttaccgtt
     1021 ataaggatat cttagtgttg ctaggagata tggatgctta tcaattacaa attgggccta
     1081 tctttgataa gtttgaaatc ccctactatt taggaaaagc agagcctatg gctgcccatc
     1141 cattagtcca atttatagaa tcgctcgaac gaagtcagcg ctataattgg cgtcgtgaag
     1201 acattttaaa catgttaaaa tcaggacttt ttggttgttt tgatgatagt gatattgatc
     1261 gctttgagga atatactcag tttgcagata ttaaagggtt tactaaattt tcaaagcctt
     1321 ttaccattaa ctcttctagg cagtatcctc ttgacttttt aaatgaaatg agacaagaca
     1381 tcgttttacc tttgcaagaa ttgtttaaga gtcaaaaaca acttggtgct tcacttgttg
     1441 acaaattaat acttttttta aaaaagatta gattagctga gaatatgcaa ggtttagcac
     1501 agtcccaatt agaggtggaa aaaaacgaag aagtttggaa aaggtttaca gatattttga
     1561 cttcttttca tcatatcttt ggtcaagaaa aattacggct atcagattgc cttgctttaa
     1621 tcaaaacggg aatgaaatca gctcagtatc gtgttgttcc agcgacatta gatgttgtca
     1681 ctattaaatc ttatgacctt gttcaaccac attcaaaacc tttcgtttat gcaattggtc
     1741 taactcaatc acattttcct aagcaaattc accattcagg actactttct gatcaagagc
     1801 gtgctagaat taatgaaata agaaattatc gtcattttga tattgctagt gccgaaaata
     1861 gtaaaaaaaa ccatcaaact gcgttatctc tgtttaatgc tgctactaaa gagttagttc
     1921 ttagtgttcc aacggtaatc aacgagacat tcgatgatct atctccttat cttaaagaat
     1981 taataaactt tgggttacct cttttggata aaggcaaaaa ctatctatct tatgataata
     2041 gcgatattgc taactataaa gctctcttgt cacaaattat tgctattaac cgacaagatc
     2101 ttatagaaat gtcagatcaa gataagatgt tttggactgt tgtcttacgt tatctcagga
     2161 aacagttaag aaagcaacag ctagagctac ctacttcaga ttatcgttta tcaacaaagc
     2221 ctttgtctaa agaagttatc gaggtatgtt ttccaaaggg aatcccttta aagctatctg
     2281 caactgcttt aaccgttttt tataataatc agtacaacta ttttttgaaa tatgttttaa
     2341 atttaaacaa aacagaatcc attcacccag attccagaat tcatggtcaa tacttgcatc
     2401 gtgtctttga gcggttaatg aaagatcaca cgcaagaacc gtttgataat aagttaaaac
     2461 aagcaatcta ccataccaat caagaaagtt tttttcagca ggtttaccaa gataatgccg
     2521 aggctgagta cagtttagct atactagaag atattgtcag atcaacagca cctatcttgc
     2581 aacttaacca aaacatacag gtcattgatc aagaaaagaa ctttcagctg gatatgggaa
     2641 atgagatatt agtccacggg atcattgatc gtattgacca gctgagcgac ggtagcttgg
     2701 gcattgttga ctataaatca agcgcgaatc aatttgatat tgggaccttt tataatgggt
     2761 tgagtccaca actagtgact tacctcgcag ccctaaaaca gatcgcaccg cacgatataa
     2821 accaattatt tggggctatg taccttcatt tgcaagatcc taaattggat ctagtaacat
     2881 ttaaacaaat tgataacaca ttagtggaga gtatctataa agcgttgacc tataaaggta
     2941 ttttttcaga agtcgaaaaa gagcatctat ctactggtgc ttaccaaacc aaaaatgctc
     3001 tttattccaa tgacgaattg gaaacacttt taaattataa caagtacctc tatcttaaag
     3061 ctgctaaaca cattaaaaag ggacattttt tgattaatcc ttatacaagc gatggtaaga
     3121 cagttcaagg agatcaatta aaagccatta cccgttttga agcagatctt gacatgggac
     3181 aggcaaggcg actagtgact ctccctgcta aggagaaaaa agagtgcttt ttaacattaa
     3241 tgagaaagga gagccacttg tgatttcttt tgccccattt ttaagccccg aagctattaa
     3301 acatttacaa gaaaacgaaa ggtgcagtga tcagtctcaa aaacgcacag ctcaacaaat
     3361 tgaagcaatt tatactagtg gccaaaatat acttgtatca gcttctgctg gttcaggaaa
     3421 aacctttgta atggtcgaac gcatacttga taaaattttg agaggtgttt caattgatcg
     3481 gctttttatc tcaaccttta ctgttaaagc agctacagaa ctgcgtgaac ggattgaaaa
     3541 caaattatac tcacaaattg ctcaaactac agattttcaa atgaaagttt atttaacaga
     3601 acaattgcaa tctctttgtc aagctgatat tggtactatg gatgcttttg cacagaaagt
     3661 agtaagtcgc tatggttata gcattggcat ttcatcccaa tttcgtatca tgcaggataa
     3721 agcagaacaa ggtgttttaa agcaagaggt gtttagcaaa ctctttagtg agtttatgaa
     3781 tcaaaaagag gcaccggtgt ttagggctct tgtgaaaaat ttttctggta actgtaaaga
     3841 cacttcagct tttagagagt tagtttatac ttgttattct tttagccaat cgacagaaaa
     3901 tcccaaaata tggttgcaag aaaattttct aagcgctgct aaaacttacc aaagacttga
     3961 agatatcccg gatcatgata ttgaactctt acttttggca atgcaagaca ctgcaaatca
     4021 gctaagagat gtgactgata tggaagatta tgggcagctg actaaggcag gtagccgatc
     4081 tgctaaatac actaaacact taacgatcat agaaaagttg tctgattggg tgcgtgattt
     4141 taaatgtttg tatggaaaag ccggattgga tcgattgatc agagatgtga caggccttat
     4201 accatctggg aattatgtta cagtctcgaa ggtaaaatac cctgttttta agaccttgca
     4261 tcaaaaatta aaacaattta ggcatttaga aacaatttta atgtatcaga aagactgttt
     4321 tcccttattg gaacagttac aagattttgt gcttgcgttt tcagaagctt atttagctgt
     4381 caaaatacaa gaaagtgctt ttgaattttc agatattgca cactttgcaa tcaaaatttt
     4441 agaggaaaat acggatattc gccaatccta tcagcaacac tatcatgagg tgatggttga
     4501 tgaatatcaa gataacaatc atatgcaaga gcgactcctg accttactat cgaacggtca
     4561 taatcgcttt atggtaggag atatcaaaca atcgatctat cgatttcggc aagccgatcc
     4621 tcagattttt aatcaaaagt ttagagacta tcaaaaaaaa cctgagcagg ggaaagtgat
     4681 tttactcaaa gaaaactttc gtagccaatc agaggtgtta aatgtcagca acgctgtttt
     4741 tagtcactta atggacgaat cagtaggaga cgtcttatac gatgagcaac atcagttaat
     4801 agcaggtagt catgctcaaa cagtccccta tctagaccgt cgtgctcagt tattgctata
     4861 taatagcgat aaagatgatg gcaacgcccc ttcagatagt gagggtattt catttagcga
     4921 ggttacaatt gttgccaaag aaattattaa gcttcacaat gataagggtg tcccttttga
     4981 agacattacg ttactcgttt cttcaagaac aagaaatgat atcatttctc atacattcaa
     5041 tcaatatggt attcctatag taacagatgg tgggcagcaa aactatctta aatctgttga
     5101 agtgatggtt atgttagata cattacgcac cattaataac ccaagaaatg attatgccct
     5161 tgtggcttta ctgcgctcac cgatgtttgc ctttgatgag gatgatttag caagaatagc
     5221 acttcaaaaa gacaatgagc tagataaaga ttgcctatat gacaagatac aaagggctgt
     5281 gattggaaga ggtgctcatc ctgaattgat tcacgatacc ttgcttggca agttaaatgt
     5341 ttttttaaag acgttgaaaa gctggcgtcg atacgctaag ctagggtcgt tgtatgactt
     5401 gatttggaaa atttttaatg atcgttttta ttttgatttt gtagctagtc aagcaaaagc
     5461 agaacaagca caagctaatc tatacgcatt agctctacgt gctaatcagt ttgaaaaatc
     5521 gggctataaa gggctatacc gttttattaa aatgattgat aaggtacttg agacgcaaaa
     5581 tgacttagct gatgtggaag tggctgctcc taaacaagct gttaatttaa tgaccattca
     5641 caagtctaaa ggtttacaat ttccgtatgt atttatcctt aattgtgaca agcgcttctc
     5701 aatgacagat attcataaat catttattct gaatcggcag cacggtatcg gtatcaagta
     5761 ccttgcagat atcaaaggtt tacttggtga aacaacactc aattctgtta aagtaagcat
     5821 ggaaacctta ccttatcaat tgaacaaaca agagttgcgc ttagcaactt tatcagaaca
     5881 aatgcgctta ctgtatgttg ctatgacacg agctgaaaaa aaagtttatt ttattggtaa
     5941 agctagtaag agcaaaagtc aagaaatcac agatcctaaa aagttagaca aacttttgcc
     6001 gctggcttta cgagaacagt tattgacatt ccaagattgg ctattagcaa tagcagatat
     6061 attttcaact gaagatcttt attttgatgt tcgctttatt gaagatagtg atttgacaca
     6121 agagtcagtc ggacgacttc aaacaccaca gttattaaat ccagatgatc ttaaagataa
     6181 tcgtcaatca gaaacaattg cacgggcttt agatatgtta gaagcagtgt ctcaattgaa
     6241 tgccaattat gaagcagcta ttcatttgcc aacagttcga acgcctagcc aacttaaggc
     6301 agcttacgag cctttattag aacccattgg tgtagatatt atagagaaat cttctcgatc
     6361 gctatctgat tttactttgc cacatttttc aaaaaaagca aaagttgaag caagtcatat
     6421 tggatcagct cttcatcagt tgatgcaggt gctccctttg tcaaaaccga taaatcaaca
     6481 aacgctttta gacgctttaa gaggaattga tagtaacgaa gaggtaaaaa cagctcttga
     6541 tctcaaaaaa atagagtcgt tcttttgtga tacaagccta ggccaatttt ttcagactta
     6601 ccaaaaacac ttgtatcgag aagcaccatt tgctatttta aaagttgacc ctattagtca
     6661 agaagaatat gtcctacgtg gtattataga tgcctacttt ttgtttgatg atcatattgt
     6721 attagtggac tataaaacag ataaatacaa gcagcccatt gagttaaaaa agcgttacca
     6781 acaacagttg gagttatatg cagaagctct cactcaaacg tataaacttc ctgtgactaa
     6841 gcgctatctt gttttaatgg gaggtggaaa gccagaaatt gtcgaagttt aaggttgttg
     6901 tgacacaagg gtattttgca aatgataata gtagagttga ggattttacg aaatgcttga
     6961 aaagctattg ac
//
LOCUS       DAUELZ010000085         6947 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.086, whole genome shotgun sequence.
ACCESSION   DAUELZ010000085 DAUELZ010000000
VERSION     DAUELZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.086"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            300..377
                     /locus_tag="VHI09_001356"
                     /pseudo
     CDS             300..377
                     /locus_tag="VHI09_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(475..2352)
                     /locus_tag="VHI09_001357"
     CDS             complement(475..2352)
                     /locus_tag="VHI09_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ6121229.1"
                     /translation="MSHFLVEKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDASSVLDTVLSSDVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKPVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDAANLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRIFYGNYSDYLDEKAFEKETVEADLAKAIVTEEVPLSPKEEKKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(2349..3557)
                     /locus_tag="VHI09_001358"
     CDS             complement(2349..3557)
                     /locus_tag="VHI09_001358"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEQ6121230.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQREAGLNHLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDH
                     IEALDQALAIHNKHDIVLNGSHLIKDFGMEPGPQLGLMLEKVELAIVEGCLDNDFTTI
                     EAFVREELAT"
     gene            complement(3682..4530)
                     /locus_tag="VHI09_001359"
     CDS             complement(3682..4530)
                     /locus_tag="VHI09_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ6121231.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            complement(4530..4910)
                     /locus_tag="VHI09_001360"
     CDS             complement(4530..4910)
                     /locus_tag="VHI09_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEQ6121232.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            complement(5064..5314)
                     /locus_tag="VHI09_001361"
                     /pseudo
     CDS             complement(5064..5314)
                     /locus_tag="VHI09_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5813..6832
                     /gene="ideS"
                     /locus_tag="VHI09_001362"
                     /pseudo
     CDS             5813..6832
                     /gene="ideS"
                     /locus_tag="VHI09_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin G-degrading enzyme IdeS"
BASE COUNT         2169 a         1451 c         1126 g         2201 t
ORIGIN      
        1 ttgacttaac ttttttactt gatttttcct cacgtaataa tagaaaagaa gcgccataat
       61 gtaaactaca ccccaaacgt tagataaaaa aatctaacgt ttggggtgtt tttgcatgac
      121 attaagctat gaagataagc tagatattta gtgctaaaaa aaccgaggaa actccgtttg
      181 aaagaagcat ctctgaaaag aagaagacaa aaaatcatta taaagaatgg acaaagacga
      241 gttctgatta gattttcttt taagaacggc taaattgact cgttctacct actattatta
      301 tcgattattg caacaataac cgtatcaaaa taaaacgaaa aggacttagt cctctacaac
      361 acagaactaa atcctttact tcaaatagag tagccaactt ttggggctca gtgcaagagc
      421 acttcttttc tattatcact tgtttattat aaacataaca ttaccatagt atacttatat
      481 gtctagtcca ctcaaatact caaaccgttc atacgccaat aataaatcat tattgcgctg
      541 atcgagttcc ttttgtaatt gagctaactg gccataatca ctaactacag ttaacatttg
      601 gttttcaatt tcttcaatat tagcctcaat agttgctatt ttatcttcta tctgagccca
      661 ttcttgtttt tcaaggtagg acatgcgctt tttctcctct ttcggagaaa gtggcacttc
      721 ttcagtgact attgctttag ctaagtcagc ctcaactgtt tctttttcaa aagctttttc
      781 atcaaggtaa tcactgtaat tcccataaaa aatacgaata tcgccttctt caaaggctaa
      841 aatcttagtg gctactttgt ctaggaaata acgatcgtga ctaaccgtaa tgactggtcc
      901 tgcaaagtta gctaaaaagt tttctaagac cgtcaaggtg gcaatgtcta aatcatttgt
      961 tggctcatca aggagaagaa catttggctt ttcaatgagc agttttagca agtagagccg
     1021 ttttttctcc ccaccagata atttagcaat cagcgtgcca tgacttgatc tcggaaaaag
     1081 aaattgctct aaaagctcac tgatactcgt tgttcccacg cttgttttaa cctcatcagc
     1141 cacttcttga aggtaattaa tgacccgttt ggtctcatcc atatctttga gctgctgaga
     1201 gaaataacct acacgaatag tatctccaat atcaagcttc ccagaagttg gtttgagatc
     1261 accgttgatg atatttaaca gggtagattt tccgacgccg ttatctccaa caattccaat
     1321 acgatcttta ttttgaataa tcaagttaaa atccttaatc aactgtcggt cgccataagc
     1381 aaaagaaagg tcttcaaagt gaatcacctt cttcccaatt cggctcgttt cgaaagccat
     1441 ctccaactta tccgcagaac tgtcttggtg aacttctttt ttcaagtcag tgaaacgatt
     1501 gattctagcc tgctgtttag ttgcacgcgc ctgtggctgc gtcctcatcc aagctaattc
     1561 ttgtttatag agttgcttct tcttgtgtaa attagcagca tctcgttcgt cttgttctgc
     1621 ttttaagcgc acatagtctt ggtaattgcc ttgatattct gtcagtcccg ctttatccaa
     1681 ttcaaaaata cgggtagcca catgatcaag gaaataacgg tcatgggtaa taaaaaggac
     1741 gggtttttta gctgtcttga gataggttgt taaccaagcg atggtgtcaa tatcgagatg
     1801 gttggtcggc tcatccaaca agagtaagtc agcagctcct agcaatactt gagctaactg
     1861 gacacgtctt ctcataccac ctgataggtc tcccactttt tgttccaaat ccgtgatgcc
     1921 taattttgat aggactgttt tgacatcact ttcaatagac caagcatcta agcgatccat
     1981 ttcagacatg agggactcta aggtctcttg tttatcttca gtataattcg ccatcaatag
     2041 ttcatactga cgaatcagct gaatcgcctt aacatcagat gacaataccg tatctaaaac
     2101 agaggatgca tcgttaaatt caggatcttg tgttaagtaa gcgattttgt aatcattggc
     2161 tttagaaaat ggtgaatggt ctccgtcaaa accaagcctt cccgacaaga catctaaaag
     2221 agttgttttt cctgttccat taaccccaat aatcccaata cggtcaaagt catgaataat
     2281 aaaggaaata tcctgaaaga cggtcttatc tcccaccgtt tttgtcaatt tctccaccaa
     2341 aaaatggctc atgttgctaa ttcctctcta acaaatgctt caattgtagt gaaatcatta
     2401 tctaggcatc cctctacaat cgctaattca actttctcaa gcataagccc tagctgagga
     2461 cctggttcca tcccaaaatc cttaatcagg tgagaaccat taaggacgat atcgtgtttg
     2521 ttatgaatgg ctagcgcttg atctaaagct tcaatgtggt ccatgtcaac agataagctt
     2581 tgagcttttc ggaggccttc cactagactt gccatttcct tgccgtactg gtaaatatcc
     2641 aatttttcaa aagatctttc ctgacgaatt ctataaagag caatcaactt tgtgacataa
     2701 cgctgaaaat catttgaagt cttccatgct tttaggaagc ttttaggatc ctcaatggct
     2761 aaactaatca tcacataagc ccaagcttgg tggtgatcac taaaggtaaa gttatctttc
     2821 aaagaaacta tcagatgatt caagcctgct tcccgctgct tcaagcctgg taaatagtca
     2881 taagcctgac aagcaatcat ggcagaaatc ccttttcgcc agtgcggtgc catcaataat
     2941 ttatcaaatt cagtaaaaga acgttccaca gaaatctttt ccaatagagg tgaatggctc
     3001 ctcatagctt caaaagttgc agcttcaatg tcaaaatcca gactcgccgc aaatcgaaag
     3061 cctctcataa tgcgtagtgc atcttcttca aaacgttcct ctgctttacc cactgctcgt
     3121 aaccgttttt gctccaaatc aatgagtcct ctaaatttat caataacttg accattttca
     3181 tccaaggcca aggcattgac ggtaaagtct cttcgtttta agtcttcctc taaagagcgc
     3241 acaaaagaaa cctgactagg acggcgataa tcaacataga catcttctgt cctaaaggtt
     3301 gtaatttcat attcaccacc attttccaaa accaaaacag tgccatgttc aatgccaaca
     3361 tcgacagttc tgttaaaaat cgctttagtc tcttcaggat aggaactagt tgcaatatct
     3421 acatcatgaa tgggacgctc gagtaaaaca tctctgacgc ttcctcccac aaaataggct
     3481 tcataaccag cttcttttat tttagtcaat atgggtaaag ccttctgaaa ctcagaaggc
     3541 attgtcatta atttcatacg ttgttttcta acctatcatc caactagccc aatctaaagt
     3601 gtgattaagc tacttgggtt ctatcacatt ttgttattgt tttagtgatt cttgcaatta
     3661 actatttttt ctaatcgaca attaaagcct cggtgttacg ccaaatgcaa tggctccttc
     3721 tcctaaatga gtagcaatca ccgctccaaa atgaacctct tcaatgtcat attgataacc
     3781 actgtcctgt aagagtcgtt tcaaataatc agctttgtct tgtgcttttg agtgaataat
     3841 agacacatta tactgaccgt ctgctatcaa gtcatttaaa atctcaacca agcgtttcat
     3901 ggctttcttt tctgttctca ctttttcata aacaacgatt tttccctctt catcaaagcg
     3961 taaaataggt ttaatactca aaagatttcc aagaagagca gacccatttg acaggcgtcc
     4021 gcctttaacc agatgattaa ggtcatcaac cataataaag gctgtcgtcc catcaatctg
     4081 ttcttgcagc ttgtttaaaa tagcttgaaa tgtcattcct tgacgtgacc aatctagcac
     4141 attttttacc atactaccta aaggagcaga tgtgatctta ctgtctggaa atgccatttc
     4201 aatctcagga tgttcttcag ctaaaaattg aatattttgc caaaagcctg agattcctcc
     4261 agctaagaac aagccaataa catgtgtata acccttagaa gacaaaagtc ctaaaagatt
     4321 atctagttca gataaacttg gctgacttgt cttgggaaga ttttgggagt cagccatctt
     4381 ttgataaaaa tcatctatac tcaaatttcg accttcaaaa taggtctcat cgtcaatgat
     4441 aactggtata tcaaggctaa aaatagcttt atcttgtttc agatctgttg gtagtgttgc
     4501 tgtactgtca gtaattactg ctaatttcat taatttttaa actccaaatg aatacctgga
     4561 cgatccaagg caatttctgt gacttcataa gtcaatcgct taaccatatc tagcaaagga
     4621 gcagctaagg attcaacttc ctcttgcgta aattcgcttg gtttatccac taaacgatct
     4681 aaaatacgaa ccatttgtga gatgacccca ctaataacaa attcttcttt gacaataaca
     4741 aattgcaata cagatacaat tactgtttct ttttgctgct catctttatc aatcaactga
     4801 aaagtcactt caaaattggt ctctggtgtt ccgttttctt tttcccactc taaatttcta
     4861 gcatcataat ggtactggtt gacaaattct ttttcacgaa ctaattgcat tttttattct
     4921 ccttggtatc aatatgatcc attatagcat agatttgaaa attattaaag tagcccctaa
     4981 actactattt ttgcataacg ttttaaacag tgctatctct cgaacagata tctatttttt
     5041 cacctattta tatttagtcg tccttatact gacattatat caaaaaaaca cccgttagtg
     5101 gtcttgtact gcacctcaat taaaacagaa ccactggtca ggtaagtttt aagtccagga
     5161 taaaaaggtt tatcaccaag gcctcatcat gcttagccaa gtatttcgaa ctagcttgtc
     5221 ttagctctag tcgctcaaac gtaaatccta ggcgttcaat ccgccgcaaa acctctccaa
     5281 ctaacccata cttaaaccca tagggcttaa tcatcgctcc gcccttcttc ttttatccat
     5341 tataaaataa aaacgtttta acagacaaaa aataggaaag taccagacta ttggatttat
     5401 ttttttatct caaactccat taacgatgtt gagttaaagg cattcaaaac aacatagatc
     5461 gtaagctaac aagatttttt atcaattgaa cctatcgtcc ccagttatac cacgacctct
     5521 tctggatgag gtcttttttt atttgtctaa tttcatcaac gcttcttccc ttctttcttc
     5581 tatcacagct ctcctattgc tcacttcctc atgcagcaac atatcatcaa aaaaccgttc
     5641 atttttaagc acaaccacta ctgtatctct tgtgatgtct ccattgcaat cgcttgttta
     5701 attttattaa aaattattta aaaaattaaa tattatttta agaacttgca ataatatatg
     5761 tctatattat ataatttatt tatcataaaa ttatatagaa aaaggtattc ttatgagaaa
     5821 aagatgctat tcaacttcag ctgcagtatt ggcagcagtg actttatttg ctctatcggt
     5881 agatcgtggt gttatagcag atagtttttc tgctaatcaa gagattagat attcggaagt
     5941 aacaccttat cacgttactt ccgtttggac caaaggagtt actcctacag caaacttcac
     6001 tcaaggcgaa gatgtttttc acgctcctta tgttgctaac caaggatggt atgatattac
     6061 caaaacattc aatggaaaag acgatcttct ttgcggggct gccacagcag ggaatatgct
     6121 tcactggtgg ttcgatcaaa acaaagacca aattaaacgt tatttggaag agcatccaga
     6181 caagcaaaaa ataaacttca atggcgaaca gatgtttgac gtaaaagaag ctatcgacac
     6241 taaaaaccac cagctagata gtaaattatt tgaatatttt aaagaaaaag ctttccctta
     6301 tctatctgct aaacacctag gagttttccc tgatcatgta attgatatgt tcattaacgg
     6361 ctaccgcctt agtctaacta accacggtcc aacgccagta aaagaaggta gtaaagatcc
     6421 ccgaggtggt atttttgacg ccgtatttac aagaggtgat caaagtaagc tattgacaag
     6481 tcgtcatgat tttaaagaaa aaaatctcaa agaaatcagt gatctcatta agaaagagtt
     6541 aaccgaaggc aaggctctag gcctatcaca cacctacgct aacgtacgca tcaaccatgt
     6601 tataaacctg tggggagctg acttcgattc taacgggaac cttaaagcta tttatgtaac
     6661 agactctgat agtaatgcat ctattggtat gaagaaatac tttgttggtg ttaattccgc
     6721 tggaaaagta gctatttctg ctaaagaaat aaaataagat aatattggtg ctcaagtact
     6781 agggttattt acactttcaa cagggcaaga tagttggaat cagaccaatt aaagcgtatc
     6841 aataagaaag aataccttca acagatgatg gtattctaag gactaatact attgcaggga
     6901 atttttttat agatgggcaa cagcaaaaaa cattaataaa tcctata
//
LOCUS       DAUELZ010000086         6895 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.087, whole genome shotgun sequence.
ACCESSION   DAUELZ010000086 DAUELZ010000000
VERSION     DAUELZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.087"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..1132
                     /gene="dnaN"
                     /locus_tag="VHI09_001363"
     CDS             <1..1132
                     /gene="dnaN"
                     /locus_tag="VHI09_001363"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEQ6121233.1"
                     /translation="QFSINRTLFIQALNATKRAISTKNAIPILSSIKIEVTPTGVTLT
                     GSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQHQV
                     VLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRPIL
                     TGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDDIE
                     TVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAMERA
                     FLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYLIE
                     SLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN"
     gene            1207..1404
                     /locus_tag="VHI09_001364"
     CDS             1207..1404
                     /locus_tag="VHI09_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEQ6121234.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            1734..2849
                     /gene="ychF"
                     /locus_tag="VHI09_001365"
     CDS             1734..2849
                     /gene="ychF"
                     /locus_tag="VHI09_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEQ6121235.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMIYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            2919..3488
                     /locus_tag="VHI09_001366"
     CDS             2919..3488
                     /locus_tag="VHI09_001366"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEQ6121236.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVISHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            3491..>6895
                     /gene="mfd"
                     /locus_tag="VHI09_001367"
     CDS             3491..>6895
                     /gene="mfd"
                     /locus_tag="VHI09_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEQ6121237.1"
                     /translation="MDILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLRNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GIFINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFADNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYERLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEHEIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQIGSLSKYVSADGKEPKINKLNDGRFQKTKQ
                     KVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVAELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKILTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTIRQKGNTEINLQIDAYLPDDYIADERQK
                     IDIYKRIREIQSREDYLNLQDELIDRFGEYPDQVAYLLEMALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDV"
BASE COUNT         2330 a         1064 c         1328 g         2173 t
ORIGIN      
        1 tcaattttca attaatcgca cattatttat tcaggcttta aatgcaacta aacgtgctat
       61 tagcactaaa aatgccattc ctattctttc atcaataaaa attgaagtca ctcctactgg
      121 agtaacttta acagggtcta acggtcaaat atcaattgaa aacactattc ctgtaagtaa
      181 tgaaaatgct ggtttgctaa ttacctctcc aggagctatt ttattagaag ctagtttttt
      241 tattaatatt atttcaagtt tgccagatat tagtataaat gttaaagaaa ttgaacaaca
      301 ccaagttgtt ttaaccagtg gtaaatcaga gattacctta aaaggaaaag atgttgacca
      361 gtatcctcgt ctacaagaag tatcaacaga aaatcctttg attttaaaaa caaaattatt
      421 gaagtctatt attgctgaaa cagcttttgc agccagttta caagaaagtc gtcctatttt
      481 aacaggagtt catattgtat taagtaatca taaagatttt aaagcagtag cgactgactc
      541 tcatcgtatg agccaacgtt taatcacttt ggacaatact tcagcagatt ttgatgtggt
      601 tattccaagt aaatctttga gagaattttc agcagtattt acagatgata ttgagaccgt
      661 tgaggtattt ttctcaccaa gccaaatctt gttcagaagt gaacatattt ctttctatac
      721 acgtctctta gaaggaaatt atcccgatac agaccgttta ttaatgacag aatttgagac
      781 ggaggttgtt ttcaataccc aatcccttcg ccacgctatg gaacgtgcct tcttgatttc
      841 taatgctact caaaatggta ctgttaagct tgagattact caaaatcata tttcagctca
      901 tgttaactca cctgaggttg gtaaggtaaa cgaggattta gatattgtta gtcagtctgg
      961 tagtgattta actatcagct tcaatccaac ttaccttatt gagtctttaa aagctattaa
     1021 aagtgaaaca gtaaaaattc atttcttatc accagttcga ccattcaccc taacaccagg
     1081 tgatgaggaa gaaagtttta tccaattaat tacaccagta cgaacaaact aagtaagaaa
     1141 aagctccctt ttaggagttt ttttgttatt ataaatatta acgataatat aagtggagaa
     1201 aagggaatgt atcaaattgg atcatttgtt gaaatgaaaa aacctcatgc ttgtgtaatt
     1261 aaagaaactg gtaaaaaggc taatcaatgg aaggtactga gagtaggagc tgatattaaa
     1321 attcagtgta ctaactgtca gcatgtgatt atgatgagtc gttatgattt tgagcgaaaa
     1381 ttaaaaaaag tactgcaacc atgaaagtcc ttttaaataa ataattctag ctatatttgc
     1441 aaccaatact ttcctaaaaa attgttagta tgccgttgga aaattagcta ttctaatgtt
     1501 atcgaaagaa gaaaggtggc tattgacaat gacgaaagtt gtagcacagt tacttagttt
     1561 ctcggcaaac tatatcaaaa tgagaataat acaccaaact ttgataattt aaatgaaggt
     1621 gaaagagtgg cataatgata agcaaatctg aaattttctt cttattcata ccgctttttc
     1681 ttctattttt tggtataata atcttgattg aaatttgaat ggagattcgc taaatggctt
     1741 taacagcagg tattgtaggc ttaccaaatg ttggtaaatc aaccttattt aacgcaatta
     1801 caaaagcagg agcagaagct gctaattatc cttttgcgac tattgatccc aatgttggta
     1861 tggtagaggt tccagatgag cgtctgcaaa aattgacaga attgattaca cctaaaaaaa
     1921 cagttccgac aacctttgaa tttactgata ttgcaggtat cgttaaaggt gcttctagag
     1981 gggaaggtct aggaaataaa tttttagcta atattcgtga agtcgatgct attgttcatg
     2041 tggtacgtgc ttttgatgat gaaaatgtca tgcgtgaaca aggtcgtgag gatgctttcg
     2101 ttgatcctat agcagatatt gacactatta atcttgaatt aatcttagct gatttagagt
     2161 caatcaataa acgttatgcg cgtgttgaaa aaatggcacg aactcaaaaa gataaagaat
     2221 cagtagcaga gttcaatgtt ttacaaaaga ttaaacctgt tttggaagat gggaaatcag
     2281 ctaggacaat tgagtttaca gaagaggaag caaaagttgt taaaggtctc tttttattaa
     2341 caactaaacc tgttttatat gtagctaatg tcgatgaaga taaagttgct aatccagatg
     2401 gtattgatta tgtcaaacaa attcgtgact ttgcagctac tgaaaatgct gaagtagttg
     2461 ttatctcagc gcgtgcagaa gaagaaattt cagagcttga cgatgaggat aaagaagaat
     2521 ttttggaagc tatcggtctt actgaatcag gcgttgataa attaaccaga gcagcttatc
     2581 atctcttagg ccttggaacc tattttacag caggtgaaaa agaggttcgt gcttggacgt
     2641 ttaagcgtgg tataaaagct ccacaagctg ctggtattat ccattcagat tttgaaagag
     2701 gttttattcg tgcagtaacc atgtcttatg atgatctaat gatctacggt tcagaaaaag
     2761 ccgtcaaaga agctggacgc ttgcgtgaag aaggaaaaga atacgttgtt caagatgggg
     2821 acatcatgga attcagattt aatgtgtaat tatattaaaa caatatcaga aggttggaag
     2881 aacattccag cccttttggc attttagaaa gagaaaatat ggtaaaaatg attgttggtc
     2941 tgggaaatcc aggctctaaa tatgaaaaaa caaagcacaa tattggtttt atggctattg
     3001 acaatattgt caagaacctt gacgttacct ttacagatga taaaaatttt aaagcacaga
     3061 taggaagtac ttttattaat catgaaaaag tttactttgt gaaacctact acttttatga
     3121 ataatagcgg catagcagta aaagcattac taacctacta taatattgac ataacagatt
     3181 taattgttat ctatgatgat ttagacatgg aagtcagtaa attacgttta cgtagtaagg
     3241 gttcggcagg aggacataat ggcattaagt caatcattgc ccacattgga actcaggaat
     3301 ttaaccgaat caaagttggt attggacgac ctttaaaagg tatgactgtt attagccatg
     3361 tgatgggcca attcaatacc gaagataata ttgctatttc gttaactctt gacagagttg
     3421 tcaatgctgt caagttttat ttacaagaaa atgattttga aaaaacaatg cagaaattta
     3481 atggataatc atggatattt tagaattatt tagtcagaat aagaaagtcc aatcctggca
     3541 ctctggatta accaccttag gaagacaact ggtaatgggg ttatcgggtt caagtaaagc
     3601 attggctata gcttccgctt atttagatga tcaaaaaaaa atagttgtgg ttacatcaac
     3661 tcaaaatgag gttgaaaaat tagccagcga tttatctagt ttacttgatg aagaacttgt
     3721 tttccaattt tttgcagacg atgtggctgc agcggaattt atctttgcgt caatggataa
     3781 agctctatca agaatagaaa ccctgcaatt tttaaggaat cctaaatctc agggcgtttt
     3841 aattgttagt ttatcaggct taagaacttt attgccaaac ccagatgttt ttacaaagag
     3901 tcagattcaa ctaacagttg gagaagatta tgatagtgat actcttacta aacaactgat
     3961 gacaattggc tatcagaagg tctcacaggt tattagtccg ggagaattta gccgtcgagg
     4021 ggatatttta gatatctatg agattacaca agaattgcct tatcgattgg aattttttgg
     4081 cgatgatatt gatagtatta ggcaatttca tccagaaact caaaaatctt ttgaacaact
     4141 agaaggtatt tttattaatc cagcaagtga tcttattttt gaggctagtg attttcaacg
     4201 tggcattgag caattagaaa aggctctaca aacagcacaa gatgataaaa aatcttattt
     4261 agaagatgta ttagctgttt caaaaaacgg ttttaaacat aaggatatcc gtaaatttca
     4321 atcattattt tatgaaaaag agtggtcatt attagattat attcctaagg gaacgccaat
     4381 cttttttgat gattttcaaa aactagttga taaaaatgca agatttgatt tagagattgc
     4441 taatctcttg acagaagatt tacagcaagg aaaggctctt tccaatctta actactttgc
     4501 agataattat cgagagctta ggcactataa gccagcgacc ttcttttcaa attttcataa
     4561 gggacttgga aatatcaaat ttgatcagat gcatcagcta actcagtatg ccatgcagga
     4621 attttttaat caatttcctt tgttgattga tgagattaaa cggtatcaaa aaaatcaaac
     4681 aacggttatt gtacaggtag agtctcagta tgcttacgaa cgacttgaaa aatcttttca
     4741 agattaccaa tttcgccttc ctttagtgag tgctaatcaa attgtttcac gtgaatcaca
     4801 aattgtaatt ggagctatct ccagtggttt ttattttgct gatgaaaagt tagcgttgat
     4861 cacagagcat gaaatttatc ataaaaagat caaacgacgc gctagacgat ctaatattag
     4921 caatgctgag cgtttgaaag attacaatga gttagcagta ggtgattacg tggttcataa
     4981 tgtccatggt atcggtcgct ttcttggaat tgaaacgatt cagattcagg gaatccatcg
     5041 agattacgtt actattcaat atcaaaattc agaccgtatt tctcttccaa ttgaccaaat
     5101 tggtagctta tcaaagtacg tttctgctga tggaaaagaa cctaaaatta ataaactcaa
     5161 tgatggtcgt tttcaaaaga caaagcaaaa ggtcgctaga caagtagagg atattgctga
     5221 tgaccttcta aaattatatg ctgaaagaag tcagcaaaaa ggattttcat tttcaccaga
     5281 tgatgacttg cagcgcgctt ttgatgatga ttttgctttt gtagaaacag aagatcaact
     5341 taggtctata aaggaaatta aagctgatat ggagagcatg caacctatgg atcgtctttt
     5401 agtaggcgat gtaggatttg gtaagacaga agtagctatg agggcagcct ttaaagcggt
     5461 gaatgatcac aaacaagtag ctgtcttagt tccaaccaca gtcttggccc agcagcatta
     5521 tgaaaacttc aaagcacgct ttgaaaatta ccctgttgag gttgacgtct taagtcgttt
     5581 ccgtagtaaa aaagagcaag ctgaaacact agaacgtgta cgaaaaggtc aaattgacat
     5641 tattatcgga acccatcgac tcctatcaaa agatgtggtc ttttctgatt taggattaat
     5701 tgtgattgat gaggaacaac ggtttggtgt taagcataaa gaaactttaa aggaattaaa
     5761 aactaaggtt gacgtcttaa ccttaacagc tactccgatt cctagaacct tacacatgtc
     5821 tatgttaggt atccgagatt tatcggttat tgagacccca ccaaccaatc gttatcctgt
     5881 tcaaacctat gttttggaaa ataatccagg tctcgttaga gaagctatca ttcgtgaaat
     5941 ggatcgtgga ggacaaattt tttacgttta caataaagtt gacactattg aaaagaaagt
     6001 tgcagagcta caagaattag tcccagaagc ttctatcggt tttgttcatg ggcaaatgag
     6061 tgaaattcaa cttgaaaata ccttgattga ctttataaat ggtgattatg atgtccttgt
     6121 ggctacaaca atcatcgaaa caggagttga catttctaat gtaaatactt tgtttattga
     6181 gaatgctgat catatgggat tgtcaacttt atatcaactg agggggcgcg tcggaagaag
     6241 taatcgtatt gcctatgctt acctgatgta tcgtcctgat aagatcctaa cagaagtctc
     6301 tgaaaaacgt ttagaggcta ttaaaggctt tactgaatta ggttcaggct tcaagattgc
     6361 tatgcgagat ttgtctatac gaggagcagg taatatttta ggagcttctc aaagtggctt
     6421 tattgattcg gtcggttttg aaatgtattc tcaattatta gaacaggcta ttgctagcaa
     6481 gcaaggaaaa acgactattc gccaaaaagg taatactgaa atcaatcttc agattgatgc
     6541 ttatttacca gatgattata ttgcagatga gcgccaaaaa attgacattt acaagcgtat
     6601 tcgagaaatt caatcaagag aagattatct caatttgcaa gatgagctga tagatcgttt
     6661 tggagagtat cctgatcaag ttgcctattt gttagagatg gctctgctaa aacattatat
     6721 ggacaatgcc tttgcggaat tagttgaacg caaaaataat caggtcattg ttcgatttga
     6781 agtaacttct ttaacttatt tcttaactca ggattatttc gaagccttat cgaaaaccca
     6841 tcttaaagct aaaattagtg aacatcaggg gaaaatcgat atcgtctttg acgtt
//
LOCUS       DAUELZ010000087         6569 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.088, whole genome shotgun sequence.
ACCESSION   DAUELZ010000087 DAUELZ010000000
VERSION     DAUELZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.088"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            517..1044
                     /locus_tag="VHI09_001368"
     CDS             517..1044
                     /locus_tag="VHI09_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEQ6121238.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            1044..2162
                     /gene="yqeH"
                     /locus_tag="VHI09_001369"
     CDS             1044..2162
                     /gene="yqeH"
                     /locus_tag="VHI09_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEQ6121239.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIERINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGDKDVITTSRFPGTTLDKIEIPLDDGSFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNSEQTLFLGGLSRFDFINGERQ
                     GFTAFFDNQLELHRTKLDGADAFYDKHVGTLLTPPGKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            2187..2495
                     /gene="yhbY"
                     /locus_tag="VHI09_001370"
     CDS             2187..2495
                     /gene="yhbY"
                     /locus_tag="VHI09_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEQ6121240.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            2564..3196
                     /locus_tag="VHI09_001371"
     CDS             2564..3196
                     /locus_tag="VHI09_001371"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEQ6121241.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDFYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            3193..3786
                     /gene="yqeK"
                     /locus_tag="VHI09_001372"
     CDS             3193..3786
                     /gene="yqeK"
                     /locus_tag="VHI09_001372"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEQ6121242.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECSDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLQDKEILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAQLDLDKA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEK"
     gene            3813..4022
                     /locus_tag="VHI09_001373"
     CDS             3813..4022
                     /locus_tag="VHI09_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEQ6121243.1"
                     /translation="MIGMLTNYCMDMTVRVAFELGYEVSVIEHGSTTFDDEDIQASLL
                     IDYHESLWDGNFARVEPLDVILNEE"
     gene            4024..4377
                     /gene="rsfS"
                     /locus_tag="VHI09_001374"
     CDS             4024..4377
                     /gene="rsfS"
                     /locus_tag="VHI09_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEQ6121244.1"
                     /translation="MKKEELLEIVVKAADEKRAEDMLALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHIEGDSQAGWVLLDLTDVVVHIFSEDERYHYNLEK
                     LWHEAPAVALDAYLA"
     gene            4425..5168
                     /locus_tag="VHI09_001375"
     CDS             4425..5168
                     /locus_tag="VHI09_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ6121245.1"
                     /translation="MEHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLTIAEKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEPEVGDVFKEVYDVLADDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEAPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTETSKRWFFVAYK"
     gene            5422..6528
                     /locus_tag="VHI09_001376"
     CDS             5422..6528
                     /locus_tag="VHI09_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEQ6121246.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTENVID
                     YQKLIKVYEKKSKQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILIYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKKERLN"
BASE COUNT         2047 a          988 c         1441 g         2093 t
ORIGIN      
        1 gtaatcattt tttgatttac agttattatc atgtgttatc tggcgactgt cctgtttaca
       61 agtaagttca caagtatggg ataaaatagt ataaactata gtaaatactg aatagtcata
      121 gaaaaatatc ggcataggtc agaagagctg atatgtttaa cactttttgc ggatatctac
      181 tgtatttaaa tgtatttgat atccttgtca aggatatacg tctaaacaag ttcaaaacga
      241 aattatttgt gattatctta tcaggagttg actcttgata agatttttgt atattagtac
      301 tgtggcacgt ttagatagtt tggcagtgaa tttatagagt gtcaaacaac gagtgttttg
      361 tgttacacta accattagaa gagtctattg ttgagcatat caaggtgtta ccttttcgag
      421 ttgaggtatc atatctagta tatttaacta tggttttaaa tgctatttca aaaaaaatat
      481 gataaaatag aaagtactaa ttattttgga gatgacatga gtatcgatga ttataggccg
      541 acctatatgg tagaggctat ttatgattta agagcaaatg atttgttgcg tcatggtatt
      601 acggctgttt tagtggattt agataatacc ttaattgcat ggaataatcc tgatggcaca
      661 ccagaggttc gcgcttggct tgatgaaatg actatcgcgg atattagcgt ggttgttgtt
      721 tcaaataata aacattctcg tgttgagcgt gccgtttcac gctttggagt tgactttatt
      781 agccgggctt tgaagccatt tgcttatggt attgaaaaag ccattgctcg ttatggattt
      841 gatcgcaacg aggttatcat ggttggagat cagctaatga ctgatatcag agctagtcat
      901 agagctggaa taaaatcagt tttagtcaaa ccacttgttg cttctgatgc ttggaatact
      961 aaaattaacc gctggcgtga acgccgtgtg atggcaaaat tagaagagaa atatggtaaa
     1021 ttatcatatc aaaaaggaat ttaatggaag aattattttg tataggctgt ggtatccaga
     1081 ttcaaactga agacaaagaa aaagctggtt ttacgccagc tgctgctttg aaaaagggta
     1141 tggaaacagg agaactctat tgtcagcgct gctttagatt gaggcattat aatgaaatta
     1201 cagatgtcca tattaccgat gatgaatttt taagacttct tcacgaagtg ggtgatagtg
     1261 atgctcttgt ggttaatgtg attgatattt ttgactttaa tggttctatt attccaggtc
     1321 tatctcgctt tatttctggt aatgatgtct tactggttgg gaataaaaaa gatattttgc
     1381 caaagtctgt taaggatggt aaagttactc aatggttgac ggaacgtgca cacgaagaag
     1441 gtcttcgccc tttagacgtt atgttaacga gcgctcaaaa taaatatgcg attaaagact
     1501 tgattgagcg tatcaatgag ttacgtaatg gtcgagatgt ttatgttgtg ggggtcacta
     1561 atgttggtaa atcgacctta attaatgcca ttattcaaga aattacaggg gataaagatg
     1621 tgattacgac gtcccgcttc cctggaacga cccttgataa aattgaaatc ccactggatg
     1681 acggtagctt catatttgat acgccaggta ttattcatcg tcatcaaatg gctcattatt
     1741 tatcccctaa agagctaaaa atagtgagtc ctaagaagga aatcaagcct aagacgtatc
     1801 aattaaactc agaacaaact ctctttttag gtggtttgtc tcgctttgac tttatcaatg
     1861 gggagcgtca agggtttaca gctttttttg acaatcaact agaattacat cgtacaaaat
     1921 tagatggagc agatgctttt tatgataagc atgttggaac tttgttaact cccccaggta
     1981 agaaagagtt gacagcattt cctaaattag ttcgtcatga atttacaatt gatcagaaaa
     2041 tggatattgt tttttctggt ctaggatgga ttcgtgttaa tggtcaaaaa gactcaaaag
     2101 cgatagttgc tgcatgggct cctgaagggg tcgctgtcat tgttcgtaaa gcgattattt
     2161 aaaaataaaa aagaagttgg aaaaaaatgc ttacaagtaa acaaagagct ttcttaaaat
     2221 ctgaggctca tagcttaaaa ccgattgtac aaattggcaa aaatggatta aatgaccaca
     2281 ttaagactag tattcgtcaa gctttggatg ctagagaatt gataaaggtc actttgttgc
     2341 aaaatactga tgaagatatt catgaagtag cagagatttt agaagaagaa attgggtgtg
     2401 atacggtttt gaaaattggt cgtatcttga ttctctataa ggtttctgct aagaaagaaa
     2461 accgcaagtt gtcccctaaa gttaaagcaa tttagtcctt ttatttgtat aggggctggt
     2521 cttatgagac gaggactatt gtcgcgagaa ttttggagaa agtatggcat tagaattgtt
     2581 gacgcctttt acgaaggtgg aattagagga agaaaaaaaa gaaagcaacc gcaaacagat
     2641 tgggatttta ggagggaatt ttaatcctat tcacaatgca caccttgtgg ttgcagatca
     2701 agttcgccag caattaggat tagatcaggt gctgttgatg cctgaatgta aaccaccgca
     2761 tgtggatgcc aaagagacta ttgatgagaa gcatcgtctt cgcatgttgg aattggctat
     2821 tgaagatgtt gaagggctag ctattgaaac ctgtgaactt gaacgtcagg gaatcagcta
     2881 tacctatgat acgatgcttt atttgactga gcagcatcct gatgttgatt tttattttat
     2941 catcggagct gatatggttg attatttgcc aaagtggcac cgtattgatg aattggtcaa
     3001 actcgttcaa tttgttgggg ttcaacgtcc aaaatataaa gctggaacct cttacccagt
     3061 catttgggta gatctacctt tgatggatat ttcttctagt atgattagag attttattaa
     3121 aaaaggtcgt cagcccaatt atctcttacc taagcgggtt ttagactaca ttactcaaga
     3181 aggtttgtat caatgacata tgaagattat cttccttata gcaggaccga actgttagca
     3241 aaaattgcag agcagatgag ccctaaacgg tttaaacatg tacttggcgt ggaaaaggca
     3301 gccttgtcct tggcagagtg ttatggttgt aacccagaca aagcagggtt agcagctctt
     3361 ttacatgatt atgccaagga atgctctgac caggtttttt tagacttgat tgacaagtac
     3421 caattatctc cagaattagc taaatggaat aataatgtct ggcatggtat ggttggtatt
     3481 tataagattc aagaagacct agggttgcaa gataaagaga ttttgcgagc tattgaaatt
     3541 cacaccgttg gtgctgctga gatgaccttg ctggataagg tcttatacgt tgctgattat
     3601 attgaagaag gtcgcatctt tcctttggtt gatgacgcta gaaaaatcgc gcagcttgat
     3661 ttggataagg ctgttgccta tgaaacagtg aacactgtcg cctatttagc ctcaaaagcg
     3721 cagcctattt ttccacagac cttagataca tataatgcgt tttgcagtta tttaaaggag
     3781 aagtaattga aaaaggcatt gataacttgg taatgatagg aatgctcact aattattgta
     3841 tggatatgac agtgagggtt gcttttgaac tgggctatga ggtgtcagtg atagaacatg
     3901 gttcaacaac ttttgacgat gaggacattc aggcttcact cctgattgac taccatgaga
     3961 gtttgtggga tggtaatttt gctcgagtag agcctttaga tgtgatttta aatgaggaat
     4021 aaaatgaaaa aagaagaatt attagagatt gtggttaagg ctgcagatga aaagcgggct
     4081 gaagatatgt tagccctcga tttagaaggt ttaaccagtt tgactgatta ttttgtgatt
     4141 gcgagtgcaa caaatagtcg tcaattagag gctattgcgg ataatattcg ggaaaaggta
     4201 aaagaggctg gtggagatgc tagccatatt gaaggtgata gtcaagcagg ctgggtcttg
     4261 cttgatttga ccgatgtggt ggtacatatt ttttctgagg atgagcgtta ccactataat
     4321 cttgaaaaat tgtggcatga agcgcctgct gtggctctag atgcctattt agcttaacgt
     4381 tacaaagaga ccttgtcaat acgacagggt ttttgaggat gataatggaa cataattatg
     4441 aaaaatttgc atcagtttat gatgccgtga tggacgactc tttatatgac ttgtggacag
     4501 atttttcact tcgacattta ccaaagtcta agggtcgaaa tcgtttgtta gagttggcct
     4561 gtggaactgg tattcagtca gttcgttttg ctcaagcagg ttttgacgtg actggtttag
     4621 acttaagtca agacatgttg actattgctg aaaaacgggc tcagtctgct aaaaagaaaa
     4681 ttgattttat tcaaggcaat atgctggatt tatcacaggt ggggcagttt gattttgtca
     4741 cttgctattc ggattccatt tgttatatgc aagatgagcc tgaggtaggt gatgttttta
     4801 aagaagtcta tgatgtttta gcggatgatg gcatctttat ttttgatgta cattcaacct
     4861 atcaaacgga tgagtgtttc cctggttatt cctatcatga gaatgcagat gattttgcaa
     4921 tggtttggga tacctatgct gacgaggctc ctcactctgt tgttcatgaa ctcactttct
     4981 ttatccaaga agacgatgga cgtttttctc gctttgatga agtgcatgag gaacgaacgt
     5041 atgagttatt gacttatgat attttgttgg aacaagcagg gttcaagtca ttcaaggtct
     5101 atgcggattt tgaggataag gagccgactg agactagcaa gcgttggttt tttgtggcat
     5161 ataaatagaa gaaaaaagta gcctttttag ctataggatt taatgaatta acttgaatgt
     5221 tacttatatg aaaagagttt attaggcatt aaatgctatt atcaaagctt agaagtattg
     5281 gagacattac gattgacact atgtctgaag gttgctacca actttaaaga aagcattttc
     5341 tattatacgt tgtgcttaag catttgattt aactcttatt agccctataa aagattattt
     5401 agtacaattt gaggagattc tatgactgtt accggaatta tagctgagtt taatccgttt
     5461 cacaatggac acaaatattt gctagaaaca gcagagggac taaaaattat tgctatgtca
     5521 ggaaatttta tgcaaagagg agagcctgct cttattgata aatggattcg atcagagatg
     5581 gctcttaaaa atggtgcgga tattgttgtg gaattgccct ttttcgtttc agttcaatca
     5641 gcagactatt ttgctcaagg agctattgat attctttgtc aattaggaat tcagcaatta
     5701 gcatttggga cagagaatgt gattgattat caaaaactga ttaaggtcta tgagaaaaaa
     5761 tcaaagcaaa tgactgccta tttatcgacg cttgaagata cacttagcta tccacaaaaa
     5821 acacaaaaaa tgtgggaaat ctttgctgga gttaaatttt cagggcaaac gccaaatcat
     5881 attttggggt taagttatgc aaaggctagc gctggaaagc atatacaact atgtcctatc
     5941 aagcgtcaag gggctgccta ccattcgaaa gacaaaaatc atctgctagc ttctgcaagt
     6001 gccattagac aacacctgaa tgattgggat ttcataagtc attcagtacc aaatgcaggt
     6061 ttacttataa acaatcccca tatgtcatgg gaccattatt tttcattctt aaaatatcaa
     6121 attctgaacc attctgattt aactagtatt tttcaggtca atgatgaatt ggcaagtcgt
     6181 attaaaaaag cgataaaagt tagtcaaaat attgaccacc tagttgacac tgttgctacc
     6241 aaacgctata caaaagctcg tgtaagacgt attttaatct acattttggt taatgctaag
     6301 gaacctactt tacctaaggg aattcatatt ctagggttta cctcaaaagg acaagcacat
     6361 ttaaaaaaat taaaaaaaag taggcctctg attactagaa ttggagcaga aacttgggat
     6421 gagatgacac aaaaagcaga cagcatctat caacttgggc atcaggatat cccagaacaa
     6481 agttttggaa gaatacctat tataataaaa aaagagaggc tcaattaaac tctaattgag
     6541 tttttttggg ggcttgactc taacattac
//
LOCUS       DAUELZ010000088         6428 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.089, whole genome shotgun sequence.
ACCESSION   DAUELZ010000088 DAUELZ010000000
VERSION     DAUELZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.089"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..447)
                     /locus_tag="VHI09_001377"
     CDS             complement(<1..447)
                     /locus_tag="VHI09_001377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121247.1"
                     /translation="MLKLTPPCFSVKDILDELLDGVTKSKEFTDDQGNRIDSKKYTEL
                     FNDSVKNRIFEFEVIYIKLAESGTLHSLRQSDCRVSEEIGKEEMEFLYKQKLVTKYKD
                     SYYLRLRTNENKNFNQCVYCERDLVSDLDHLLPQSEFPIFAVTPANL"
     gene            complement(440..1975)
                     /locus_tag="VHI09_001378"
     CDS             complement(440..1975)
                     /locus_tag="VHI09_001378"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ6121248.1"
                     /translation="MTFKVVETYWDIQNDGYYLVHSNWDDWFKYETTYLFYIKKNNQT
                     TNVGSIKIGQFSMVEGQRVPNLPEEFDSLTDDFFSVATDESFYYEINRLESEKREYIL
                     TALKDVAFDNDLYHKAVKTDVFNVSLTRDLLPQVIVNKYRRLANGNTKLSNYDFSFKM
                     SDACTLNFKVSPTEFPASNIHAIIGRNGVGKSYLLTEMVESAIQNDGRFSFNTEFDDN
                     FFSNIISVNFSSFENKKLFEENHEASKGYLYYHLSNRTTAKESKNYKLYKEEDGEIVE
                     YVPEGWDAVFIESVSTALRKKPKLWRDCIKILYSDPIFSQIGIDTLVETFQQEDDIRK
                     FYKEAYKIFRGLSSGHKIVILTITKLVEMVSEKTLVILDEPELHLHPPLLSSFIRSLS
                     QLLIKANGVAILATHSPIILQEVQKECVYKLSREMSTDFMQAVRPSNETFAEDIGSLT
                     FDIFELEAIESGFYTLINQIVKENLSFEDSLQRFDGRMGTLGYKLLLTEILKRDKEEG
                     RNA"
     gene            complement(1978..3075)
                     /gene="purK"
                     /locus_tag="VHI09_001379"
     CDS             complement(1978..3075)
                     /gene="purK"
                     /locus_tag="VHI09_001379"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEQ6121249.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSACQLPQGTDLLRISQNR
                     IVEKDFLANKAGVTVAPYKVMTSSLDLGGLDLTKTYVLKTATGGYDGHGQKIIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTVFPVQENIHCNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVTENPSAHLHMYGKLE
                     AKHNRKMGHVTVLKEDEVEFGEEINWKYGEI"
     gene            complement(3062..3553)
                     /gene="purE"
                     /locus_tag="VHI09_001380"
     CDS             complement(3062..3553)
                     /gene="purE"
                     /locus_tag="VHI09_001380"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEQ6121250.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKYAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            complement(3831..5096)
                     /gene="purD"
                     /locus_tag="VHI09_001381"
     CDS             complement(3831..5096)
                     /gene="purD"
                     /locus_tag="VHI09_001381"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEQ6121251.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVRPVLEGMVAEGRPYLGVLYVGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQNDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENSKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDPTGLFYRNDIGSKAIRE"
     gene            5349..6428
                     /locus_tag="VHI09_001382"
                     /pseudo
     CDS             5349..6428
                     /locus_tag="VHI09_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
BASE COUNT         1838 a         1454 c         1193 g         1943 t
ORIGIN      
        1 caaattagct ggggtaacag cgaaaatagg aaattcagat tgtggtaata aatggtctaa
       61 atcgcttact aagtccctct cgcaatacac acattgatta aaatttttat tttcatttgt
      121 tctcagtctg aggtaatacg agtctttata tttcgtaact aatttttgct tgtagagaaa
      181 ttccatttct tccttaccaa tttcctctga tactctgcaa tcgctttgac gtaagctatg
      241 tagagtacct gattcggcaa gttttatgta tattacttca aattcaaata ttctattttt
      301 tacactatca ttaaataatt cagtatactt tttagaatct attctattac cttgatcatc
      361 cgtgaattct ttgctttttg ttacaccatc taataactca tccaatatat ctttcacact
      421 aaaacaaggt ggagtaagtt taagcatttc taccttcttc cttgtctctt tttaaaattt
      481 cagttagtaa caatttatat cctaatgtcc ccattctacc atcaaatcgc tgtaatgaat
      541 cttcaaatga aagattttct tttactattt gatttattaa agtataaaat cctgattcta
      601 ttgcttccaa ctcaaatata tcaaacgtta aactccctat atcttcagca aaagtttcat
      661 tacttggtct tactgcttgc ataaaatcag tgctcatctc cctagataat ttataaacac
      721 attccttttg tacttcctgt aagataatgg gagaatgcgt tgctagtatc gcaacaccat
      781 tcgcttttat aaggagttga gataaacttc tgataaagct actcaacaaa ggtggatgca
      841 agtgtaactc tggctcatct aaaataacaa gtgttttttc tgagaccatc tctactagct
      901 tagttatggt taaaattaca attttatgac ccgagctcag acctctaaaa attttatacg
      961 cctctttata aaacttacga atatcatctt cttgttgaaa tgtttcgact aatgtgtcaa
     1021 ttcctatttg actaaatatt ggatcactat ataaaatttt tatgcaatct cgccataatt
     1081 ttggtttctt tcgtaatgcc gtactcacac tttcaataaa tactgcgtcc catccctcgg
     1141 gtacgtactc tactatttca ccatcttctt ctttgtatag cttataattt ttagactctt
     1201 ttgcagttgt tctgtttgat aggtgatagt acagataccc cttactagct tcatgattct
     1261 cttcaaaaag ctttttgttc tcaaagctac taaaattaac agagataata ttcgagaaaa
     1321 aattatcgtc aaactcagta ttaaaactga aacgaccatc attttgtatc gcgctttcta
     1381 ccatctcagt taacaaatat gatttaccta caccatttct accgataatg gcatggatat
     1441 ttgaagcagg aaattcagtg ggactcactt tgaaattcaa tgtacaagca tcagacattt
     1501 tgaaagaaaa atcataattt gataatttgg tatttccatt agctaatcgt ctatacttat
     1561 taacaattac ttgaggtaag agatccctcg ttaaagacac attaaaaaca tcagttttta
     1621 cagctttatg atacaaatcg ttatcaaagg caacatcttt taaagcagtt aaaatatact
     1681 ccctcttttc actttcaagc ctgttaatct cgtaatagaa actttcatcc gtggctactg
     1741 aaaaaaaatc atcagttaaa ctgtcaaatt cttcaggtaa attgggaact cgttgtcctt
     1801 cgaccataga aaactgacca atcttaattg aacccacatt tgtagtttga ttattctttt
     1861 ttatataaaa tagatatgtt gtttcgtatt taaaccaatc atcccagtta ctatgtacca
     1921 aatagtaacc gtcattttga atatcccaat acgtctccac tactttaaaa gtcatattta
     1981 aatttctcca tacttccaat taatctcttc cccaaactct acttcatctt ctttcaacac
     2041 cgtcacgtgt cccattttgc ggttatgttt cgcttctagt ttaccataca tgtggaggtg
     2101 ggcgctaggg ttttcagtca catagtcaat tgcctgctgg acgtgttgtc cgaggacatt
     2161 aagcatgacg gctggagcat ggaggtggat ggttgggagc ggcgctccta gcacgcccaa
     2221 aatgtgggtg tcaaactgtg aaaagtcgca agcctcgatt gagtagtgcc ctgagttgtg
     2281 gggacgtggg gcaatttcat tgacaatgat atcatctgcg gtcgcaaaca tttccacaca
     2341 gagagttcct gacagctgga gtttcttggc aatctgcacc gccattttct tagccttatc
     2401 agccagttga tctgagatgc gagctggcac gatagttttt gacaggatat tattgcagtg
     2461 gatattttcc tgaacgggaa agaccgtcac atcctctccg tttccagaca cgataactga
     2521 gatttcaagg tcgaaattga caaactcttc caagacacac tgagctgagt tagctaattg
     2581 ctgcgcctct ggcaaatctt ctgctgagcg gataatcttt tgcccatgac cgtcataacc
     2641 acctgtcgct gtcttgagga cataggtctt ggtcaaatca agccccccta ggtcaaggct
     2701 agacgtcatc accttatagg gagcgactgt cacaccagcc ttatttgcca gaaagtcttt
     2761 ttcaacgata cggttttggg aaatgcggag cagatcggtc ccctgcggta gctgacaagc
     2821 tgacacaacg gcatccaaac cgtccgcatc aacattctca aactcataag tcaatacatc
     2881 gcaacgcgca gccaattgtc ccaaagcctc aacatcatcg taaggcgcca caattacctc
     2941 gctaacgcgg gaggcagggc agtcgctagc aggatccagc gtgatgacct tgtggcccat
     3001 gtagatagcc gcaatcgcca tcatctgccc cagctgacca ccaccaataa tcccaatggt
     3061 cttaaatgag ttcaccactt gactcctctg cgatttttcc ttgttcctca tggaagtgag
     3121 ctagcgcatc agccagattt tgatcctcga tggagagaat acggagggct gtcagggcag
     3181 cgttagtcgc tcccgcttct ccgatagcca tggttgccac aggcacaccg ccaggcatct
     3241 gcacaatcga atagagtgaa tccaaacctg aaagggcacg tgatttgaca ggcacaccga
     3301 taacaggcag cgttgtttta gctgccacca taccaggcaa gtgagctgcc ccacctgctc
     3361 ctgcgatgat gattttgatg ccacgaccac gtgcttcctc agcatacttg aacatgaggt
     3421 cgggcgtgcg gtgagcagag acgactttct tctcgtaagc aatgccaaag ttgtctagaa
     3481 cttcagccgt tttttgcatg gttgcccagt cagatttgga gcccatgata atagaaataa
     3541 ttggagtttt cataattaag atacaaaggg gcgtttcgac ttgtcgaaac tttccgtaga
     3601 aaaatatgcg gtaagtcaga aatctttgat ttcgttgacg cacccacgtc aggacgacta
     3661 ggtgctttag catcagacgc ctacgacgca gaaaactaac gatgacgctt gcgtccaggt
     3721 agttttattt ttttcctagg aaattagtcc cgtgttcagt tcctttcttc tttctttttg
     3781 gagcgacctt cggtcgttga ttgctgacgc taatatttga atggttagtc ttattctctt
     3841 atagctttgc ttccgatgtc atttcggtag aagagacctg ttgggtcttg ttgggccagt
     3901 tgggtataga ttttgtcctg ccctgctttg acgctgtctt ctgtggtgac aagcatatag
     3961 acgcgtcctc cgtttgagag tagcgctttg ctattttctg caaacttagc tccggcatag
     4021 taggtgatga tatcgccgtc ggtcttttca ggcagtggca cacctttctc ataatcgagt
     4081 ggatagcctt ctgaggcaac gacaacaccc aaagtcacac cgtcattctg ccaagtgatg
     4141 taaggctcaa tgcccatcat gatatcgtca atattctgag cgaaatcgga agtcagacga
     4201 gggaggataa tctgagtttc agggtcaccg aaacgtgagt tgaactcgat aaccttaggt
     4261 ccatcagctg tcaggataag ccccacataa aggacaccta gataaggacg cccttcggca
     4321 accatgccct caaggacagg cctaacgatc gtctcaaccg ctgtgtctac cacgctctga
     4381 ggtagatgag gaactggcgc ataggcaccc ataccaccag tgtttggccc cttgtctccg
     4441 tcaaaggcac gcttgtgatc ctgagctgtc ggcatgatgt agaacttatc accattaaca
     4501 aaggcaaaga gggaaaactc ctcgccatcc aagaattcct cgataaccac acgcgctcct
     4561 gagtcgccaa acttattgtc cagaagcatc tcttgcgccg cctctaccgc ctgctcaacg
     4621 gtttcagcca cgaccacacc cttacctagc gccaagccat cagccttgac cacgataggt
     4681 gcgccctgct cttcgatgta ggctttggct ttttcaaagt ctgaaaatgt gccataggct
     4741 gctgttggga cattgtattt gaccatgatt tccttggcaa agtcttttga ccactctagc
     4801 tccgctgctg cctttgttgg accaaaagct ctgagtccag cactgttgaa atcatcaacg
     4861 ataccagctg ctagcgcatc atcaggacca ataaaggccc aagaaatctc attctccttc
     4921 gcaaaggcaa tcaacctgga atgttcggaa actactatat tcaccaagtc cagaccatcc
     4981 aaggtcatac catcattacc aggtgccaca aaaacctgat ccacaccctt agacgctaac
     5041 aacttcttag caatcgcatg ttcacgacca cctgaaccaa caacaagtaa tttcaaaaca
     5101 cccacctctt tcgtattata attagatatt atatcataaa cgttcgcctt ttgatacaat
     5161 gtattaatta tcaataattg aaacattgta aaaatgtgat agtagaccac aattcttgaa
     5221 ataaggaaca ctctattagc caatcaaata gttatttaat atcaagtaaa gccaattttc
     5281 aaaataatca tctaaaaata acattttttg tttcttgctt tcgaataata agttacagga
     5341 ggtaattaat gaaaaaattt catcgttttt tggtctcagg agtaatcctt ttaggtttta
     5401 atggtctagt acctactatg ccatctacac ttatttcgca acaggaaaat cttgttcatg
     5461 cagctgtttt aggcgataac tatccgagta agtggaaaaa aggcaatgga atcgattcgt
     5521 ggaacatgta tatccgccaa tgcacttctt ttgcagcttt tcgtttaagc tctgctaatg
     5581 gttttcagtt acctaaaggc tacggtaatg cctgcacgtg gggacatatc gcgaaaaatc
     5641 agggttatcc tgtgaataag acaccaagca taggggctat cgcttggttt gataaaaacg
     5701 cttatcagtc aaatgctgct tacggtcatg agcatgggta gctgatatcc gtggagacac
     5761 tgtcactatc gaagagtata attacaacgc tggacaaggc cctgaaagat accataagcg
     5821 tcaaattcca aaatctcagg taagtggtta tatccatttt aaagacttat catctcagac
     5881 aagtcattcc tacccaagac aactaaaaca catttctcaa gcttcatttg acccctctgg
     5941 aacttatcac tttacaacca gattaccagt caaaggacaa accagtatcg atagtcctga
     6001 tcttgcttac tatgaagcag gtcaatctgt ttattacgat aaagtcgtga ctgctggagg
     6061 ttatacatgg cttagctacc tcagtttttc tggaaaccga cgctatattc ccattaaaga
     6121 gcccgcacag tctgtggttc aaaatgacaa tacaaaacct tccattaagg tcggtgatac
     6181 tgttaccttc cctggcgttt ttcgtgtaga tcagcttgtt aataatttga tcgttaataa
     6241 agaattagcc ggaggagacc caactccact aaactggatt gatcccacac cattagatga
     6301 aacagataac caaggaaaag ttttaggaga tcaaattctc cgtgtgggtg aatattttac
     6361 cgtcactggt agttataaag tattaaaaat tgatcaacca agtaatggta tttatgttca
     6421 aatcggat
//
LOCUS       DAUELZ010000089         6273 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.090, whole genome shotgun sequence.
ACCESSION   DAUELZ010000089 DAUELZ010000000
VERSION     DAUELZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.090"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            6..299
                     /locus_tag="VHI09_001383"
     CDS             6..299
                     /locus_tag="VHI09_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEQ6121252.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            296..613
                     /locus_tag="VHI09_001384"
     CDS             296..613
                     /locus_tag="VHI09_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEQ6121253.1"
                     /translation="MIRPTSYEPLAALIEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFVCVNRDQFIEVAQKWNIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            631..1257
                     /locus_tag="VHI09_001385"
     CDS             631..1257
                     /locus_tag="VHI09_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEQ6121254.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEASSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPIVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMQNGSLIFPGK
                     LRGEESYGMMCSPRELALPNAPQKRGIIELDESAVVGEAFDPAKHWKG"
     gene            1409..1804
                     /locus_tag="VHI09_001386"
     CDS             1409..1804
                     /locus_tag="VHI09_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ6121255.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISVVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(2064..2705)
                     /locus_tag="VHI09_001387"
     CDS             complement(2064..2705)
                     /locus_tag="VHI09_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEQ6121256.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYKVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(2725..3702)
                     /gene="dusB"
                     /locus_tag="VHI09_001388"
     CDS             complement(2725..3702)
                     /gene="dusB"
                     /locus_tag="VHI09_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEQ6121257.1"
                     /translation="MTKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELADLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFETVR"
     gene            complement(3689..4561)
                     /gene="hslO"
                     /locus_tag="VHI09_001389"
     CDS             complement(3689..4561)
                     /gene="hslO"
                     /locus_tag="VHI09_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEQ6121258.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYASTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISHLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNESDLEALINDKA"
     gene            complement(4708..6201)
                     /gene="rofA"
                     /locus_tag="VHI09_001390"
     CDS             complement(4708..6201)
                     /gene="rofA"
                     /locus_tag="VHI09_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEQ6121259.1"
                     /translation="MIEKYLESSIESKCQLVVLFFKTSYLPITEVAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSHDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHTRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFNLQHFIPE
                     TNLFVSPYYKGNQKLYTSLKLIVEEWMAKLPGKRYLNHKHFHLFCHYVEQILRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSIDFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVQHELTKGIAVAEISFDESILSIQELMYQVKEEKFQADLTKQLT"
BASE COUNT         1951 a         1107 c         1343 g         1872 t
ORIGIN      
        1 ataaaatgag ggatatgagt aaaaagaaaa taggtatgat ttcaggtatc tttggattta
       61 gtttagctat tggcctagga atagttatca aagattattg ccaagacagg cagcgtcgac
      121 aaatgacaag ggatttacgc accttttttt cacctttagg acaaatcgaa gttttatata
      181 tcaatccttg tcaggtcaaa caagattata tttcaggtgg ggttgttatg tcaaacggca
      241 aacagtacca attcacctat cacagtcgac aaattagttt tgaggaggac aagggatgat
      301 cagaccgact tcttatgagc cattagcggc tcttattgaa aaagaagata agctggtgtt
      361 attttttacg gcagactggt gtccagactg tcaatttatt tatcctatta tgccagaaat
      421 tgaagcagaa cttacagata tgacttttgt ttgtgttaat cgtgatcagt ttatagaggt
      481 agcacaaaag tggaatattt ttggtattcc tagttttgtc gtgattgaaa aaggtcagga
      541 agtagggcgt ctggttaaca aaatgagaaa gacaaaaact gagattatgc attttttagc
      601 tgcttatcaa taagaaaaga ggaagaataa atgatttttg catacaataa agaacaagtt
      661 ggcgatgtct tgatggttat cttacaagac accaaagata tcaaacgtca agtagaacga
      721 aaaggcaaag tagcccgtgt ttttgcagaa gaaagcggca aaacccttgc ttggaatatc
      781 tttgaagcgt caagcttgat tactattgaa ggcaatggac agattttttt gacagacgag
      841 gaccttgcaa gattaaatgc agagcttgct aaggaaggat tttcagaaag gcttgaaccg
      901 attgtgggac ctgtttttgt ggttggtcaa attgttgaga tggtggctca tccagatagc
      961 gaccatctta atatctgcca agtggctatt ggtgaagatc aaacggttca aatcgtagcg
     1021 ggcgcaccaa atgctgcgct tggtttaaaa acgattgttg ccttaccagg tgctataatg
     1081 caaaatggta gtctgatttt cccaggaaaa ttgcgtggtg aagaaagtta tggtatgatg
     1141 tgctctcctc gtgagttagc cttgccaaat gcaccgcaaa aacgtggcat tattgaactt
     1201 gatgagtcag ctgtggtagg agaagctttt gacccagcca aacattggaa aggttagaaa
     1261 aaccagctga aataagactg tatttaacta aatgaccaga aaaaaaggat taggagccct
     1321 taatcctttt ttcttatgtt ttcaaaaacg tttaaaatga aacttatttt accacaattt
     1381 ttacgaataa taagataagg aggtcagtat gtataataaa gtgatagcaa tcggtcgttt
     1441 ggtagctaaa ccagaattgg taaaaacagc tacggataag catgtagcac gtctctcttt
     1501 agctgttaat cgaagattta aaaatgcttc tggagagcga gaagctgatt ttatttcagt
     1561 tgttgtttgg ggaaagttag cagaaactct ggtttcttat gctagcaaag gtagtttgat
     1621 gtctattgat ggcgaactta ggacccgcaa gtatgataaa gatgggcaag tgcattatgt
     1681 gacagaagtt ctctgccaat catttcaact gcttgaaagt cgtgctcagc gcgctatgag
     1741 agaaaataat gttactaatg atctagttga tttagtctta gaagaagata ctcttccctt
     1801 ttgatagggg ataatgcttc tcatcacggt tcagctgatt actgaaatat atgatgaggt
     1861 gatacccgag caacaaaagt gtgactagat acttctgata aaaataaaaa aacaccttac
     1921 gagtatgaac aaagtttcaa gtgctagatg ttggaacttt gttcatcata ggaatagtag
     1981 ggtgtttttt agtgtattac ttctttttgt tgctagtgtt aaggtttact tgcttacaag
     2041 cggttatcag gccactagaa ctcttataat aaatggagct ctttgagttt ggtgtcaatc
     2101 atttttaaga cagtcgccaa atcttgtggg ttttgaacga agtcaaggga attgccatca
     2161 attttcattt ttggagagac cttatagtct tcgtaccagg ttgggtattc accgtggact
     2221 tgatggtaat attgctcaag actaggattg ccgtcgacct gctcaaaact tcggccacgt
     2281 ttttcaatgc gctcaagcat tttgtcaaaa gacacatcaa tgtagatgag aagatcaggg
     2341 cgttttttag gcatgccttc tagttcttct aacatgttgg cgagaagttc ctgatagatg
     2401 tcaagttctg tcttagtgac attgccattt ttgtagttaa gttttaaaaa gagttcgtct
     2461 tcaaaaatag agcggtcaag aatgttgtta tcggcttgat aagcttcttt gatggactta
     2521 aaccgtttat tcaaaaaata aatttgaagt aaaaaggcgt actttttagg gtcttggtag
     2581 tagaggtcta gcacggggtt attatcaaca gcttcgtaaa aaacgtcagt tcctaaatgc
     2641 tctccaagtg cagcagcaag cgagctcttg ccagccccaa ttgttccagc tagtacaatc
     2701 aacatgcgtc tccttctaat atcattagcg tactgtttcg aagatggctt caacctcagc
     2761 tagagtctca gcgcgtgaaa cagcaccacg aactttggca gcacctgctg ttccgcgaag
     2821 atagtgtggt gctaagcccc gaaattcccg aacagcaatg gtttcacctt tgaggttaat
     2881 caggcgttta aggtggtctt tggcgatgtc taattttttg gcaaagggaa gatcagccaa
     2941 ttcttgacca gtttcaaaga aatgattgat ttgggtaaaa agataaggat tgttcatggc
     3001 tgcgcggcca atcatcacgg catctacacc gatttcttca atcataaatt tggcgtcttg
     3061 caccgaacgc acgtccccat ttccaataaa gggaatctta gtgatagcct tagaaactcg
     3121 ggctaatgtc tcatggtcgc aggttcctgt atacatttgt tcacgggtac gaccgtgcat
     3181 ggcaagagca gatacacctg ccgactcagc ggcaagggca ttctcaacgg caagtgaact
     3241 atccgcccaa cctgtacgca ttttgacagt cagtggaata tctaaaacag acgtgacttc
     3301 cttgacaatg tggtaaatct tatcagggtc acggagccac ttggcaccag cctcgttttt
     3361 gacaacttta ttaacagggc agcccatatt aatatcaaca atatcggcct tggtattggt
     3421 ttgaatgaaa tcagctgcac gtttcaaccc ttctgcatca ccaccaaata attgaatgga
     3481 catagggtgc tcattctcgt caatatggag catatggagg gtcttttcgt tgttgtagag
     3541 cagccctttt tcggaaatca tttccataac caccaaacca gcaccaaatt ctttggcgat
     3601 ggtacgaaag gcagagttgg tcacgccagc catgggagca aggacagtgc ggtgagggat
     3661 ttccacatca ccaatcctaa aggaagaatt aagctttgtc attgatgagg gcctccaaat
     3721 cactttcatt aaattggtac ttggtaccac aaaattggca aacaatctca gcacccttat
     3781 cttcgtcaat catggcctgt aaatctgctt ttggcaaagt cattagggct gcttcaaaac
     3841 gttctctaga acagtcacac tgaaaactca gaggttcttc agaaagacgc ttgtaaggtt
     3901 catctccata gatggcctcc aaaagggcat ccacatgatt tttggaagct agtaggtgag
     3961 aaatggcagg catttcttgc aggcgttttt catagcgagc aatctcttct tcagaagctc
     4021 ctggtagcac ctgcaccata aagccaccag caaccttgac cttatcattt tcatctaaaa
     4081 gcacattaag tccgattgca gatggtgttt gctctgattc ggttaagtag taggcaaaat
     4141 cctcaccaat ttctccagta ataagaggcg ttgttgaggc gtaagggtta cctgtgccat
     4201 aatcaataat cgtcacaaaa tgaccattcc ccataaaagg cccaacaagg acttcacctg
     4261 ttgcggtctt tttaatatcg acaccagtat tttgaatgta acctttcaca tgtcctttgg
     4321 tatctgccac agagataatg tgtccaaaag aagaatcacc aatcactttg acggtgattt
     4381 tactatcgcc tttttgatta gccgccaaaa tctggttggc gatcaaggta cggccaagcg
     4441 caacagtaga cgatgacagc gtgttgtgtt tttcttgagc taatgcaacc gtctcagtgc
     4501 tatcaaggac ataagcccta aaagcccctg attgtgctat tgatttaatt attttatcca
     4561 taacttagat tatagcataa agtgtaaaac ttgtattgac aggattgctt gaaaaatcaa
     4621 tacagataga cagtaaaaaa tcccagagaa tgactcggga ttaagttaag gtgtggtcta
     4681 aagtcgcgca atgtggtgac atggagctta tgttaattgc ttggttaaat cagcttggaa
     4741 tttttcctct ttaacttgat acatcaattc ttggatagac agaatcgatt catcaaaaga
     4801 tatttcagca acagcaattc cttttgtaag ctcatgctga acaaaaggaa tcagttgact
     4861 gtgagtgatg accaaatctg gctttaaatc aggaatttga taaacattat cttgcaatag
     4921 ataataggaa tgaaaatcaa tgcttttatc cgagaaatac cttggaaaag aatctgttag
     4981 gagatgagca ttgataaaat tactggctac gaaaacaaca actaaaggag gttggatatt
     5041 tcttagaatt tgctcgacat agtggcaaaa aagatgaaaa tgcttatggt tcaagtaacg
     5101 cttaccagga agtttggcca tccactcttc gacaattaac tttaaggacg tatagagttt
     5161 ttggtttcct ttatagtacg gagaaacgaa taagttggtc tcaggaataa aatgttgcag
     5221 attaaacaag aatgattttg aaaaaaacat aagagctttt actaaactag ccttttgctc
     5281 ttttaggtta ggtaaaagag tgatgatagg atttaaaagc aggcgaaaag tatcattttc
     5341 ttcaaaaagt tgacaacatt gtctggtatg ctcaggtgtc cattgtaagc tcgcaaaaga
     5401 attattagcg gtgatataaa ttaaatagag gtagtccaaa tctcctgctg aaaagtttag
     5461 ttggcagtaa gtttcgataa tatcatggct actttttttc aaagaatcgt agacaaaaag
     5521 tttttttaat tgttgaaaaa ttctggtttg gggaatagtt accgaaaatt gatgccgctt
     5581 ccacgataaa gccaataaaa tgtcatagaa agaaaacgat tccgataacc aaggagaggt
     5641 tttaaggtgg gtggaactat gggataaaaa gctatgaata gtgtttttgt cttgctgcgt
     5701 caagtcataa actttaatgc caaacttact atatagcaga gcgatgaggt aacggatgcg
     5761 atattcctca ccgacaatct tgttcttaga gagttttaat tcaaagtttc ttaataaagg
     5821 aatcaatgct tcgcgcatcc gataagctga ggagtttgat aaaaaatgac ttctcgcaaa
     5881 atccgtaagg ggacgagagt gggaaccatt ttttattaaa aaagctagta attgtaagac
     5941 attagatgat gcatagagtt ggtaaagata agtttcttta aaaggatgtg taaattggca
     6001 agatatcatc cttttttgga tggtcataga cagactacca gggaaaaagg cattcagttc
     6061 ctcacaataa tggtttagtt gtaaaaaggt taagccagtt ttttctgcta cctcagttat
     6121 tggcaaataa gatgtcttaa aaaacaagac aactaactga cattttgatt cgattgatga
     6181 ttccaagtat ttttctatca agtaaagggg tcctctcttc ctacacttag aaagccaaat
     6241 aagtctttga taagtgtatt ctcctatttc aat
//
LOCUS       DAUELZ010000090         6071 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.091, whole genome shotgun sequence.
ACCESSION   DAUELZ010000090 DAUELZ010000000
VERSION     DAUELZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.091"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            10..699
                     /locus_tag="VHI09_001391"
     CDS             10..699
                     /locus_tag="VHI09_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6121260.1"
                     /translation="MFSHGRLKQRRLEIKLSQTAIANQLNVHRSAYHNWENGRNTPNQ
                     KNLTALAAILDVPVTYFESEYNIVNNYLQLSPDNQLKAEEYVEELLLSQQTSNVIPLF
                     SVQVLSDIQLSAGLGEGFFDEFETETIYSDEEQYGYDIAAWIEGDSMEPVYKSGEVAL
                     IRSNGFDYDGAVYALSWNDSVYIKKLYRDEDGFRMVSLNKDYPEKFIPYEDEPRIVGL
                     VVGHFMPVEGV"
     gene            702..929
                     /locus_tag="VHI09_001392"
     CDS             702..929
                     /locus_tag="VHI09_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121261.1"
                     /translation="MKLKDILELGTYGFNPNCKVEIFCMDNFEERLENEGFDEILIPQ
                     NDDAKIYPYAFLIEDSILIAMTEVDDDINEC"
     gene            1052..2467
                     /locus_tag="VHI09_001393"
     CDS             1052..2467
                     /locus_tag="VHI09_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEQ6121262.1"
                     /translation="MGYIDYSIEPQSDIAFLDMKSFYASVECVDRGLHPLYTSLCVMS
                     RADNSAGLILASSPMFKKVFGKANVGRSYDLPFDINTRKFSYQNAWKQGLEVTPKYKS
                     FIEHWAKRTLIVPPRMDRYIEKNLEIQHIFQDYAAPDDILPYSIDEGFIDLTSSLSYF
                     ISDKSMSRKDKLDNVSAMIQRDIYSKTGIISTVGMSNSNPLLAKLALDNEAKKTATMR
                     ANWSYEDVETKVWAIPKLTDFWGIGSKTEIHLQKLGIHSIKELANFNPDILKKEFGKV
                     GVQLWFHANGVDESNVHEPYKPKSRGLGNSQVLPRDYRTQREIEIVLAEMADQVANRL
                     RSAHKKATVVSIHIGYSRTEMKKSINAQKKIDPANLPKTMVGHVLELFRKKYTSGAVR
                     QIGVSYGGFVDENFTLLSLFDDVEQIEKENRLQTAIDVVREQFGFLAIQKGTVLTEGS
                     RNIERSKLIGGHSAGGLEGLK"
     gene            2464..2831
                     /locus_tag="VHI09_001394"
                     /pseudo
     CDS             2464..2831
                     /locus_tag="VHI09_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2818..3117
                     /locus_tag="VHI09_001395"
     CDS             2818..3117
                     /locus_tag="VHI09_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121263.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVRTLEENPLIYHYTYKKTTTYLAEK"
     gene            complement(3235..4698)
                     /gene="msr(D)"
                     /locus_tag="VHI09_001396"
     CDS             complement(3235..4698)
                     /gene="msr(D)"
                     /locus_tag="VHI09_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HEQ6121264.1"
                     /translation="MELILKAKDISVEFKGHDVLDINELEVYDYDRIGLVGANGAGKS
                     TLFKVLLGELIPPGCKMNHLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKRQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEGGGRLAHQKSIGSKEKKMHNAAKSLENRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGTG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKIKLKH"
     gene            complement(4818..6035)
                     /gene="mef(A)"
                     /locus_tag="VHI09_001397"
     CDS             complement(4818..6035)
                     /gene="mef(A)"
                     /locus_tag="VHI09_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HEQ6121265.1"
                     /translation="MEKYNNWKLKFYTIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLLGFLPYAVFGPAIGVLVDRHDRKKIMIGADLIIAAAGSVLTIVAFYMELPV
                     WMVMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAAL
                     LYSVWELNAIIAIDVLGAVIASITVAIVRIPKLGDRVQSLDPNFIREMQEGMAVLRQN
                     KGLFALLLVGTLYMFVYMPINALFPLISMDYFNGTPVHISITEISFASGMLIGGLLLG
                     LFGNYQKRILLITASIFMMGISLTISGLLPQSGFFIFVVCCAIMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLTGSIMSLAMPIGLILSALFADRIGVNHWFLLSGTLIICIAIV
                     CPMINEIRKLDLK"
BASE COUNT         1934 a         1238 c         1056 g         1843 t
ORIGIN      
        1 gaggggaaca tgttttctca cggaagatta aaacaacgaa gacttgaaat aaagttatca
       61 cagactgcaa tcgctaatca attaaatgtt catcgttccg cttaccataa ttgggaaaac
      121 ggaagaaaca cacctaatca aaaaaacctc accgctctcg ctgctattct tgatgtacca
      181 gtcacttatt ttgaatctga atacaatatc gtaaataact atcttcagtt atctcccgat
      241 aaccagctaa aggcagagga gtatgttgag gaacttcttc tttcccaaca aacttcaaac
      301 gtcattcctc tcttctcagt ccaagtgcta tcagatatcc aactctcagc tggtcttgga
      361 gaaggattct ttgacgagtt tgaaactgaa acaatctact ctgatgagga acaatacggt
      421 tacgacattg ctgcttggat tgagggagat tctatggagc ctgtatataa gagtggagaa
      481 gtcgcactaa ttcgttcaaa cggtttcgac tatgatggtg cagtctatgc attatcatgg
      541 aatgactcag tctatatcaa aaaactctac cgtgatgagg atggatttag aatggtatcc
      601 ttgaataagg actatccaga gaagttcatc ccttacgagg atgaacctag aattgttggt
      661 ctagttgtag gtcactttat gcctgtcgag ggagtatagt catgaagcta aaggatattt
      721 tagaacttgg gacgtatggt ttcaatccga actgtaaagt tgaaatattc tgtatggaca
      781 actttgaaga gcggctagag aatgaaggat tcgatgaaat tcttattcct caaaatgatg
      841 atgcaaaaat ctatccttac gcttttttga tagaggattc tattttgatt gctatgaccg
      901 aggtggatga cgatatcaat gaatgttaaa gaaatgattt acatcaaaga cgaacgtatt
      961 atcttcaccc ctgacaaatt tgaatacgac atcactgact acatcggaga acttatcgaa
     1021 gagctagaga aactaaaaag gagataatcc tatgggctat atcgactatt ctattgaacc
     1081 tcaaagtgac atagccttcc tcgatatgaa gtccttctac gcttctgtag aatgtgtgga
     1141 tagaggttta catccactct acacatcact gtgcgtcatg agccgtgcag acaactcggc
     1201 aggattgatt ctcgcttctt ctccaatgtt taaaaaagta ttcggtaaag caaatgtagg
     1261 tcgttcctac gacttgccat ttgatataaa cactcgaaaa ttcagctatc agaatgcgtg
     1321 gaaacaaggt cttgaggtaa caccgaagta taaatctttc atcgaacact gggcaaagcg
     1381 tacactcatc gttcctccgc gaatggacag atatattgag aagaatctag agattcagca
     1441 tatatttcaa gactatgctg ctccagatga cattctcccc tattcaatcg atgaaggctt
     1501 tattgacctt actagctcac tttcttactt tatttctgat aagtcaatgt caaggaaaga
     1561 taagttagat aatgtttcgg ctatgattca gagagatatt tacagtaaaa caggtattat
     1621 ctcaactgtt ggaatgagta attccaatcc tcttctagct aaactagctc tagataatga
     1681 agctaaaaaa actgctacaa tgagagcgaa ctggtcatac gaagatgtag aaaccaaggt
     1741 atgggccatt ccaaaactaa cagacttttg ggggattggt agtaaaaccg agattcattt
     1801 acaaaaactt ggtattcatt caatcaaaga actagccaac ttcaatcctg atattctcaa
     1861 aaaagaattc ggcaaagttg gtgttcagct atggtttcac gcaaacggtg ttgatgagag
     1921 caacgtccat gaaccctata aaccaaaatc acgaggattg ggtaactcac aagtacttcc
     1981 tagagattac agaacccaaa gagaaattga aatcgtatta gctgaaatgg ctgaccaggt
     2041 tgctaaccgt cttcgctcgg cacataaaaa agctactgtc gtttctattc atattggcta
     2101 ttctagaact gagatgaaaa aatcaattaa tgctcagaaa aaaattgacc cagcaaatct
     2161 tccaaaaaca atggttggtc atgtacttga attattccga aagaaataca cctctggtgc
     2221 agtgaggcaa atcggtgtgt cttatggtgg ctttgtagat gaaaacttta ctctactgtc
     2281 actttttgat gatgtagaac aaattgaaaa agaaaataga cttcagacag caattgatgt
     2341 tgtcagagaa cagtttggct ttttagctat tcaaaaagga accgtcctaa ccgaaggttc
     2401 cagaaatatt gaacgtagta aacttatcgg cggtcattcc gctggtggat tggagggatt
     2461 aaaatgattg atcgttcata cttgccattt cagtcagcaa gagagtacca ggatactaag
     2521 atgcaaaaat ggatgggctt tttcctatct gagcatacat cagcactcac tgacgatgca
     2581 aacaaagtaa cgtatatgtc agatttgtca ctggagaaga aattattact cctcagtcag
     2641 gtgtatgctg ggcaactgaa cacgcgcatt catgttgtta aaaaaacaat caagtttcct
     2701 atactggaac aataccaagt ctgaccaaag atttcatttt gataaaaact acaacaggtc
     2761 acattaattt gaaattgaaa gacattgtta gtattgaact tgtagaggag gtgctctatg
     2821 aatcagcttg aatttcagcg taatcacctg caaatggact attacagcga gagctaccaa
     2881 gattttgaac gtgacttcta ccgctactcc aacatgaata ttccattgac cttcctgacc
     2941 gatgatatcc tcaaaacaat ggcgacttca cgtaagaatt actttgtcct caataaggaa
     3001 aagtccagag ataaccgcga tcacttcttc atatttgaag taagaacctt agaagagaat
     3061 ccactaatct atcattatac atataagaaa actacaacat atttagcaga aaaataggag
     3121 cagttcgatt tactgttcct atttttaatc ttcataaaat ctcaagtctt tatactcttt
     3181 aacaatggag tcgccagcca gaacagacta tactgaccag cgactaccta aaatttaatg
     3241 tttcagctta attttcttat ctctaatttc ataaactaca tcagctacat tttcgagtaa
     3301 tcgtttatcg tgggtgataa acacgatagt tccggtgtac tccttcatta gtatttccaa
     3361 agcctctaaa cttggtatgt caaggaagtt actgggttca tccattatta ggatgttata
     3421 tctacccatg agcattttag caagcaacaa ttttataatt tctccaccgc ttaaaacaga
     3481 taagcttttt ccaatatcgt tctgtttgaa ccccatagat gctagcacag aacgaatttc
     3541 tgatatattg taatcacaat ccttctgcat aaactccata acattctgat tactgttgta
     3601 cttgtaacca ttctgtgcaa agtaacctat ttttgcctta ggtgaaatag aaattccttc
     3661 ttcatggttt aagatcattt ggattaaagt tgtttttccg gttccattac cacccgttaa
     3721 tgccactttt gctcctagcg gaatttgaaa agatgcattt tcaaacagtg ccttatcccc
     3781 aaatacttta ttaatttccg caccgactat agggtatgga ttatggagct ccaatgcttt
     3841 actttgcctg aaacgaattc tgcgaatgcc ttccggagct tctacttttc ctaatgccgc
     3901 aatcctgttc tctagggatt tagcggcatt atgcatcttt ttttccttac ttcctattga
     3961 tttttgatga gctaaacgcc ctccgccttc agtacttttt ttctttgaag aaccttttgc
     4021 cttctgttct attttacgag cctgttttcg cttttcctcc gcagcccttt ccaatcgagc
     4081 acgttccgca ataaattgtt cgtattctgc agcttgtctc ttacgttctt cctctttctg
     4141 acgaagataa tcagaatagt ttccccaata ctcagtgatt ttgccatctt tcagttccca
     4201 tattttatct actatttcat caagaaaata gcggtcatgg ctaataacta acagtgcacc
     4261 tgtaaaatat tttagctgtc ctattagaaa atcaattcct tcacggtcta aatggctcgt
     4321 aggttcatcc gctaaaatac catgaacctg tgccgataag gcctgtgcta ttttaagcct
     4381 tgtttcttca ccaccgctca tggtctgtat atttaattgc tcaacaccta gcttgcctac
     4441 aagcgcaaaa tctttttcct cctgcagagt tacttcgtcc aactggggaa tataggcaag
     4501 ttcacccaga tgattcattt tacatcctgg gggaattaat tctcctaaaa gtaccttgaa
     4561 taaagtgctt tttcctgcac catttgctcc tactaaacca atacggtcat aatcatatac
     4621 ttctaattca tttatatcta aaacatcgtg tcctttgaat tccacactaa tgtcttttgc
     4681 ttttaatatt aattccataa catttcctcc tgtctataat cgcatgcttt catttgcttg
     4741 tacgcaggaa aaaccctgcg attttagcag gaagaattac atgaaaataa gatacataaa
     4801 tatccctcca atattgttta ttttaaatct aattttctaa tctcatttat cattgggcaa
     4861 actattgcaa tgcaaataat taaagtacct gatagtaaaa accaatgatt tacaccgatt
     4921 ctatcagcaa agagtgcaga aagaattaat ccaattggca tagcaagaga catgatactt
     4981 ccagttaaag aaaatacacg tcctaaatat tcaggcttaa ttttctcctg aaaaagagct
     5041 gtttgcacac cgctgtaaaa cggaacagaa agccccatta ttgcacagca gactacaaaa
     5101 atgaaaaatc cactttgggg aagtaatcct gaaatggtta agcttatccc catcataaaa
     5161 atggatgccg ttattaataa gattcgcttt tggtaattcc caaataaccc taataataga
     5221 ccccctatca acattccaga tgcaaaggaa atttccgtaa tagaaatatg cacaggtgtt
     5281 ccattaaagt aatccatgct aattaaaggg aatagtgcat taattggcat ataaacaaac
     5341 atatataatg ttccaacgag taataaagca aataatcctt tattttgccg tagtacagcc
     5401 attccttctt gcatttctct tatgaaattt gggtccaaac tttgcacgcg atcacccagc
     5461 ttaggaatac gtacaattgc taccgtaata gatgcaatca cagcacccaa tacatcgatg
     5521 gcaataatag catttagttc ccaaacggag tataagagtg ctgcaaccgc cggactaaca
     5581 atatagctta tagactgcaa agactgacta tagcctgcac atttcgtaag ctgttcttct
     5641 ggtactaaaa gtggcgtaac cgcattgaga gccggggtgt gaaaagctgt tccaatgcta
     5701 cggataaaca atactatcat aaccatccag acaggtagct ccatatagaa tgcaacaata
     5761 gtaagcaccg aaccagctgc tgcgataatt aaatcagcac caatcattat cttcttccta
     5821 tcatgacgat ccactagcac accaattgca ggtccaaaga ccgcataggg taaaaaacct
     5881 aatagtgaag ccatagacaa gaccatcgcg gatccagttt tttctgtaag gtaaaaaata
     5941 atcgccattt gcaagatggc actagtgatt aatgatactg cttgccctgc ccatattgta
     6001 taaaacttaa gtttccaatt gttgtatttt tccatttata ttatctcctg catattattt
     6061 tgcttaaatt t
//
LOCUS       DAUELZ010000091         5933 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.092, whole genome shotgun sequence.
ACCESSION   DAUELZ010000091 DAUELZ010000000
VERSION     DAUELZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.092"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(417..1400)
                     /locus_tag="VHI09_001398"
     CDS             complement(417..1400)
                     /locus_tag="VHI09_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ6121266.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGMQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKLVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTAPQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDYEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIVELVLKTVEHT"
     gene            complement(1431..2681)
                     /gene="dltD"
                     /locus_tag="VHI09_001399"
     CDS             complement(1431..2681)
                     /gene="dltD"
                     /locus_tag="VHI09_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEQ6121267.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(2674..2913)
                     /gene="dltC"
                     /locus_tag="VHI09_001400"
     CDS             complement(2674..2913)
                     /gene="dltC"
                     /locus_tag="VHI09_001400"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEQ6121268.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(2931..4187)
                     /gene="dltB"
                     /locus_tag="VHI09_001401"
     CDS             complement(2931..4187)
                     /gene="dltB"
                     /locus_tag="VHI09_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEQ6121269.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(4184..5722)
                     /gene="dltA"
                     /locus_tag="VHI09_001402"
     CDS             complement(4184..5722)
                     /gene="dltA"
                     /locus_tag="VHI09_001402"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEQ6121270.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGERRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQARMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELASGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(5734..5877)
                     /locus_tag="VHI09_001403"
     CDS             complement(5734..5877)
                     /locus_tag="VHI09_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEQ6121271.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
BASE COUNT         1991 a         1178 c         1002 g         1762 t
ORIGIN      
        1 gatcatatca gtagttttcc tgctaattga aaacaaggca tttactgtca cacaagtctg
       61 tcctttcttt ttttctacaa ctttatttcg tcagcgtaaa taaaacaaaa gctctataat
      121 ctcttgaatg ggtaaatcca ctgtagagat tatagagctt tttgaatatc aaaaaagtcc
      181 cataagattt ataatgaaaa gcaaccaaac tctcattaga aagactctta tgaaacatat
      241 caatcatacc ttacaacaca tcggaattaa agacaaaaat atcactctaa acaatgtttt
      301 gaaggatgaa tctcctactg agctcgttac tacttatcag aaaggtagat aggaaatcct
      361 gatgaagagt gtgtccaagg gattatcaat taggtcttag attacaaaat agtaattcag
      421 gtatgctcta ctgttttcaa cactagttct acaatttgtt ttcgagcttg cttagcttct
      481 cttattggaa aaataacata cgcatgaatc attttttctg caataatcag ctgattagca
      541 attcgttgtc ttgttaattg atcatgtaaa gacataatgt ctgggtaaaa aatctctttt
      601 gtcccaacaa aggtcgttat ttcaggcaaa tcctcataat caccatacaa aggactgaca
      661 taaggaagtt gcatattacc ttgaccacct gcccatagtt cacctatatc acgaagacgg
      721 cgagcatcta ggtaagcttc ttctgcttca aaatccttgt aggcttgatt tttaaaacta
      781 acatctaacc aaggagatat aagaattaat tgctttggct gtggtgctgt tttagtatct
      841 cgtatatact tagcaaacgc caaagccata cccccaccag aagaatcacc aatgacacta
      901 acttgactcg gtccattaga caagcaaaat tgctggtaag cttcaactaa tttaggatag
      961 acatcttgat aagtagcata cggtacttta ggaaagttag gaaaaacaag cttggcatta
     1021 gtcagctgta taagatgctt aaccgtctta aaatgaagtt tttcgggtcg tcgtaaatag
     1081 acactgccat aaagataata aataacaggc tgatgacttt gcttttgatc attaataata
     1141 aaaacttgca tcccgttcac atcgtattct tgagttgaaa aacctactat ctcttcagga
     1201 ttatctagat gataagggat attacgatta cgctgcgtct caaccccctt ataattatca
     1261 acttttttag ataacttata ctttctataa gcaaacattt tctcaaccaa ccaacttgaa
     1321 aaggagcgct tttgaccata ttcaactatc aaaataccta gtatcactcc aaaaaaaatc
     1381 accacagcaa taatgaccac ttattatcct cgttttctaa tattatttta ctattgcaag
     1441 tctttcctat ttgcccaaat tttactataa aaataagggt tcattttata gttgggtact
     1501 ggctgcttcg tttctagaaa tggttgcact ttcttatcaa aagctaacca gccattccaa
     1561 ccgagatgga tggtatcttg cataaagtag gactcaccac catcttttga gaagtcagca
     1621 atgcgatgaa atccttgtga ctttaactgg aattttattt tacgaactgc cgcttgatac
     1681 ttatcttgat ttaagccggt ataatccgcc caagctttat taacaggagt tataacaaag
     1741 agtacatctg tttttcgttt agcaaattct gataacaaaa gctgaaaatc attgtattct
     1801 ggtgacgcca ggtaactata attaacttgg aaattcttat aaagattgta tttaggtgct
     1861 attcgtttac gataaaatgt atttttaatc ccaaaacggt tgttggttgt tgctaattgg
     1921 cctctcttag ttgctaacgc attcaattgt ttatacgaaa atactttagg taatccctta
     1981 acgcgaggca aaattctttt ttcatagtta gtagatttgc ctaaaaaact aaaaagggac
     2041 tcttctctca atgctacttg atgttgacat ttcaaaatag ctctgtctaa ccgactaaga
     2101 gacttaccct tacttacttt tttcaataag tttgatttag acacaccagg gttaagctca
     2161 agcaaacgct ttgctgcaaa ctgtgattct ttatcagttc tagcttttag taaaaattca
     2221 atcacttgag tgttagacaa gtacatctga accgcactag gattaatccc ttgagcagta
     2281 aaccattgag gagatactac aaagatggct tttttctttt gcatttcatt ggtaatttgt
     2341 tgcataccat aataatgcga caaagatgct gatcctctct taccaattaa aaatggtcta
     2401 tagctccgct tgtagcgctc tgcaagcacc gaagggtgca tactatccat tcgactccat
     2461 tcgctagaac caaaaaaagg cacaaaacga caagtctcat ctgataaagc ttgtctttta
     2521 atcaaaccat ttttaaaaga actatctgtg atcgcaacgg catttgcttt ttcctgagct
     2581 atggaatgat caagttgtgt aggaaaacta aaaatagtaa tcactactaa aacaaaggca
     2641 ataagaagag gacctagaat taaccagagt ctcttaagca tgttgcaatt cctctacacc
     2701 ttgaacaatc ttagttactg tgttccaatc atcacgacca aattctgaaa taggtacttt
     2761 aatattgaac gttgactcta actcaacaat caattcgact gtcccaagac tatccaacac
     2821 acctgcatca aacaagtctt cgtccatcat ctctgaaaca tcttccataa acaaacggtc
     2881 aaacaattca attacagttt cttcaataga catttttata cttcctttta ttaatgtagt
     2941 ggtctgttaa accaaaggtc atttaaaaaa ccagagaata acagaagtga aaacatgaca
     3001 acgtggaaag ttaccacgat acataagaca tggaatgccc tgctttgaaa gaggggtgat
     3061 aagccttttt tcttacggtg acggttgatt tcttttttct tacgaatcca agcatcatta
     3121 ataataagtc ctataccatg gaaaagccca taagcgatat aataccaggt taagccatgc
     3181 caaaatccca tgactagcat attaaccaga taggcaacac ctgaagtgac atttcggttt
     3241 ttaaaggttt tatgcttaat caatagatga accaatcgca taaacacata atctctaaac
     3301 cagaaggaca aactcatgtg ccatctattc caaaaatctt tcaagcttgc agataagaaa
     3361 ggcatgttaa agttttctgg tgttcgaata cccaataggt aggaaatacc aatcgcaaac
     3421 atcgaataac cagcaaagtc aaaaaagaga ttcaagccat acacgtacat aaccagaata
     3481 gtttccttat taaagtagcc accaaccatc aaagccttat tttcaaggag aggaagtaag
     3541 atgccaccca aacaatagga aatgatatgc ttgtatagga aacctaacat gagatacatg
     3601 actgccttat tgagcattgc aaaataatca tcacgttcag gaagtttatg ataatcttcc
     3661 tgaaaacgtc tgaagcgatc aatggggcca cttgagaaag tggggaagaa aatcatgaag
     3721 cgaataaaat ccggtaaacg aacctcttgg agagtcccgt cacgcatctc aataatcatt
     3781 ccaatacttt taaaggtcaa atatgaaatc cctagaaaac taaagagtgt ttgagaagaa
     3841 ggacctgtta ggggagcaac tttgacccag ataaggggga ataaggccat cgcaatagct
     3901 aagtaaaaca ctcctgcact attagctttc tcacggtatt tctggtaagc aaaaacactg
     3961 atgatttgcc acaaaagata aaagagaaaa gcaactaatt gttgataatg atctcctcca
     4021 aacataaaga gaataaatac caaactcacc aaggtttcat aagtgcttaa gcgcttttgt
     4081 ttaaaaatcc caatgatgac tggtaagaaa gccaaaataa gatagacaaa gtaaatgggg
     4141 ttaccgtaag gttccatgta gggaatgtgg ctaaaaaaca tcatcatcgg ttattgacct
     4201 cattaatcaa agttttaata tctatcttac catttggagt caatggtaga ctgtctcgat
     4261 aaagaaactt agaaggcatc atataactca tcatatgatc cttaacagag gctttgatag
     4321 ctttggttaa ctccaattcc ctatcaaaac gctctttaac cccatctttg acaacgatat
     4381 aggccaagag attttgaacc ttatgttctt tattgtacct tggaacagcc acagccgatg
     4441 ctaccattgg tgattgattc aactgttggg aaacatcttc tagttcaata cgataaccag
     4501 catatttaat ctgaaaatcc aaacgcccac catacagcaa aatattatct tctgtcagtg
     4561 agccaatgtc ccctgtatga taagcaggct gtcctttaaa agtaaagaaa gcttctgcgg
     4621 ttttctcagg attgttgagg taaccttttg acacagcagg ccctgtaaca ataatttcac
     4681 cctgctcccc tgaagctaac tccttaccat cttcatcaat aatataggtc ggagaatctg
     4741 gtttggggta gccaatcggc aagcgggtat aattatccac catttctctg gtaatttcaa
     4801 tagcggataa ggctacagtc gcttctgttg gtccataggc attgataatc ttggcactcg
     4861 ggaaacgttc aaagagtttt cgagccgtag aaacggttaa ttcttcgcca tcaaaataaa
     4921 aatgcgttaa agctggcatc ctagcctggc aaaaatcatc gctaagcatg gccatatcag
     4981 caaatgaagg agtcgatgtc caaatgccaa ctggaagttg cgcaatcgtt gtaaataact
     5041 gtttaaagtc tgccaccaat tcttttggaa gtgcaaataa ggttccacct aaagccaagg
     5101 taggtgccca gtacatgaca gaaaggtcaa aggaataggg tggctgcgcc agcatttgtg
     5161 gctgtttagg tacatcaaat gctgcatctt cgatcatcca gttggtaaag ctcaaaagat
     5221 tgtcatgtga aatttgcacc ccttttggtt ggcccgttgt tccagaggtg aaaataatgt
     5281 aataattatc atctccttta acagaatgtg ttcgctcata gggcatttct gctaattttg
     5341 ctgactcaat ctctgatagt gagacaaggg aaatcccttc aatagtaaga gggaattcct
     5401 caatagcaat gattaaactg ggctttgcaa tctcaataat cgctaaaata cgttctggtg
     5461 ctgaatgcac atctactggg atataggcat gaccagattt ggttaaagct acaaaagtag
     5521 ctaacatatc ataagtttgc gccccaaaaa ccaaaactgg agattttgcc agtaaagcta
     5581 agctatctat aaatgcagca atgctatcag aatctctttt gagttgtccg taagttcggc
     5641 gttcccctaa acaatcataa actgggaaat cagcctgtgt ctgagcaaac tgctcaatac
     5701 tgtcaatcat atccttaatc atcagtcaat ctcctaaaat tcattataaa taaagttact
     5761 ttgaccatgt cctagataac caaagaaata gaccagcaac attaaaatca gataaaataa
     5821 aattgtcttg ccaataaaaa cagcaattcc tctcattctt gatttgtttt ttatcatact
     5881 ttaccttctt gtattagtga aataatgtcc taaaaaattc tactcgaaaa aac
//
LOCUS       DAUELZ010000092         5920 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.093, whole genome shotgun sequence.
ACCESSION   DAUELZ010000092 DAUELZ010000000
VERSION     DAUELZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.093"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            4..684
                     /locus_tag="VHI09_001404"
     CDS             4..684
                     /locus_tag="VHI09_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ6121272.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            complement(806..1453)
                     /locus_tag="VHI09_001405"
     CDS             complement(806..1453)
                     /locus_tag="VHI09_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ6121273.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(1456..2115)
                     /locus_tag="VHI09_001406"
     CDS             complement(1456..2115)
                     /locus_tag="VHI09_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ6121274.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLTLICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVALIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQAREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(2127..3311)
                     /locus_tag="VHI09_001407"
     CDS             complement(2127..3311)
                     /locus_tag="VHI09_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ6121275.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLVDQD
                     EYPKFGTTVDKLAKLRPAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            3432..4343
                     /locus_tag="VHI09_001408"
     CDS             3432..4343
                     /locus_tag="VHI09_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ6121276.1"
                     /translation="MNISSLAYFIEIVNCDFNLTKAAQKSHISQPALSSYIKTIEKEE
                     GVVLFQRQKGRLVGLTSVGERFFENAKVVIASHEQLMADLRSESKKYKGEISIGIPQL
                     ISGIVFSELIPKLIEENPEVKFHIVETGAYELQKKLFLKEVDFAILLTPTTLDATIAD
                     IDILLRDQLCLYMSHKTFQETFDSNPNISIKALHRQNLVTFDKTFMINHQLTTLFEEA
                     NSLPVVKCYSASWDFLLNCTRYSSYLTILPRPITHFAHMDGLVEVQLTEHPKWEVVLA
                     SLKHNKTSHLKHYIKHTILDYFTNQRF"
     gene            complement(4411..5817)
                     /locus_tag="VHI09_001409"
     CDS             complement(4411..5817)
                     /locus_tag="VHI09_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEQ6121277.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSLVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKGIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFTLLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFSVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLYSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
BASE COUNT         1886 a         1306 c          985 g         1743 t
ORIGIN      
        1 gacatggata aagaaacact aaactactgg aaaacggtta tcacatttct tcacgatgtc
       61 ttaggtgata actatgaaat tattttacat gtgattgata aaaatgatat ctatattgga
      121 gaactcgtta atagtcatat cagtggacgg tctaaacagt ctcctctcac cacctttgct
      181 cttgatttga ttaccaataa agtctataag gaaaaagatt ttgtgaccaa ttacaaagca
      241 atcgttagtc ctcagcacaa agaagttcgg ggatctactt tttttattaa agataaaaag
      301 ggtaatctcg agggaatgct atgtattaac ttagatattt cagcctatca aggagtagct
      361 agggacctct taaaacttgt caatcttaat ttagaacatt tcatcccaac tgctaaagag
      421 cctaaaacag tcacacccca gccagaagaa gctgttgaaa ttttaacctc taacatccaa
      481 gatattatcg gtcaaattat tgatccctcg ttactgagac acaatgtaca tcttagccaa
      541 gatgtcaaaa tagacattgt tgccaagctt tatgaaaaag gagtctttca actaaaagga
      601 gccgtctcaa aagtcgctga tattctatgt atttccgaac caagtgttta ccgctacctt
      661 aaaaaaattg aagccgatca ataactaaat caccataaaa cgccagttaa aagtatttca
      721 gataagcatc acaatctctg atataccgat gactgacgtt ttttttactc aatatgcctt
      781 tatggcaaga aaaggctact ggtcattaac cttcagctat gcttagaggt acacctgtcg
      841 cagcttgaac ctcatcaaaa gtaaaccctt cactgatttc aattacttgg ataccatctg
      901 gcgttacttt aaacacaccc atttctgtga taataaggtc aaccacacct tttgctgtta
      961 aaggtaaggt acattctttt aggagcttat gctttccttt attggtatgc tccatagcta
     1021 cgatgacttt tttagcacca actaataaat ccatcgctcc tcccatacca ggaaccattt
     1081 tgcctggaat caggtaattg gcgatatctc cattttctgc aatttctaaa gcacccaaga
     1141 cggtcaagtc aacatgacca ccacgtataa tgccaaaaga atctgcacta ttaaagaaag
     1201 cggctcctgg ataaaccgtc acaggctgtc ctcctgcatt aacgatagtc gaattcggat
     1261 catctgactt aggacctagt ccaataaacc cattttcaga ttgaaaatac actgtcattt
     1321 cttcaggcac aaaatttgct actttggttg gtagaccaat acctaaattt accaaagtac
     1381 catcttctaa ttctttggcc acacgctttg caatacgatt ttgaatctct tctttactta
     1441 atgcaaccgc cataactata ctccttcttt aacaatgtaa tctacaaaaa tgtgtggtgt
     1501 atgaacgcat tctggttgta tagtgccgac tggtactatt tctcgcgcct gaacaattgt
     1561 tgtttttgca caagctgcca tcaattgatt aaaattattt tcagaaccac tatactgtaa
     1621 atttcccatt tcatcagctt tattagcaaa tatcaaggcg acatctgctt caaatgggta
     1681 ttctaagaga taggtttttc ccttgatggt ttttgtttcc ttgccttctg ccactaaggt
     1741 tcctacccct gttggcgtgt aaaagccacc aatgccataa gctccgatgc gaattttttc
     1801 ggcgaaggtt ccttgaggaa tgagctcaat cgtcgtttca ccggcagtca tttggcgccc
     1861 agcttcttta tttaatccaa tatgtgttgc ataaatggtt ttgaattgat gattagccac
     1921 catttttcca acaccgcgat caacaaaacc agcatcatta cagatcaagg ttaagtcttt
     1981 tgtgcctttg tcaaccagtg catcaattaa cgcttctggt gttccattag ccataaaacc
     2041 accgaccata atcgtgtcac catctttaat atgactgata gcttctgcta ttgctattct
     2101 tttattctct ttcatacggt taccttctat cttgttacga tgatactttg accttggcca
     2161 ccaccaatac aaagcgttgc taagccatgt ctcacatcac gcttagccat ttctgctaat
     2221 aaggtgacta aaatacgagc acctgatgct ccaatcgggt gacccaaagc aatagctcct
     2281 ccattgacat taactttttc attgtcaaaa ccaaggtctc gtgaaaccgc taaagcctga
     2341 gcagcaaaag cttcgtttgc ttcaatcaaa tcaatgtcat caattgtcag ctgagccttt
     2401 gcaagagcct ttttcgtagc aggtattggt ccgcagccca taatacttgg gtctacacct
     2461 gcacttgcat aactagtgat tttagctaaa atagggagcc ctaattcttc agctttttct
     2521 ttactcatca ataaaattgc tgctgctcca tcattgattc ctgaagcatt accagcagtt
     2581 actgtcccct catctttgat aaaagcaggg cgtaactttg ctaacttatc cactgtcgtt
     2641 ccaaatttag ggtattcatc ttgatcaacg agtaaaggtt cacctttacg ctgaggaata
     2701 gtgacaggtg caatctcttc tttgaaacga cctttttcta tggcaagttg ggctttacgt
     2761 tggctatcag ctgcaaaggc atcttgttct tctctggtca gtccatactg ttgcacaata
     2821 ttttcggctg ttatacccat gtgataacct gcaaaagcat cactcaaccc atctttaagc
     2881 atggtatcta ctactttact atctcccata cgagatcccc aacgttggcc ttgtaaaacg
     2941 taaggtgctt gactcatgtt ttcggtacca ccaactaaaa caatatcagc gttgcccgtt
     3001 tgaatctttt gagcacctaa ctcaactgct tttaagccag aaccacagac catatcaatc
     3061 gtaaaagcag gggtatcata agaaagcccc gcatttagcg ctacttgccg agcaacattt
     3121 tgacccagac ctgcatgtaa cacattccca aagatcactt catcaatggc atcactttta
     3181 agaccggtat cagctaaaat ttttgtaaca actgtcaccc ccaaatctac ggctgataaa
     3241 gatttaaaga ctcctccaaa atttcctatc ggggtacgat aagcagatgt gatcaccact
     3301 tctttagtca tagaaacttc tcctttttca ttattgaaac gcttacattt ttattatata
     3361 atgtgaacta taataaacaa atatcgtttt tttatatatg ctataactaa acgttatcga
     3421 aagaggaaaa tatgaatatc tcgagcttgg cttattttat tgagattgtt aattgtgact
     3481 ttaacttaac caaagcagcc caaaaaagtc atatttcgca acccgccctt agtagttata
     3541 tcaagactat cgaaaaagaa gaaggagtcg ttttattcca gcgtcaaaaa gggcgtctag
     3601 ttggactaac ctcagtcggc gaacgctttt ttgaaaatgc taaggtggtt atcgcatcac
     3661 atgagcaatt aatggctgat ttgcggagtg aatccaaaaa atacaaagga gaaatttcaa
     3721 tcggtattcc tcaactcatc tctggcattg ttttttctga gctcattcca aaactcatcg
     3781 aagaaaatcc cgaagttaaa tttcatattg tagaaacggg tgcttatgaa ctgcaaaaaa
     3841 aactgtttct aaaagaagtc gattttgcga ttttattaac acccactaca ctagatgcta
     3901 ctattgctga tatcgacatt ctcctaagag atcaactctg cctttatatg agtcacaaaa
     3961 cctttcaaga gacgtttgat agtaatccta acatctctat caaagcatta catcgtcaaa
     4021 acttagtaac ttttgataaa acctttatga ttaatcatca gcttaccacc ttatttgaag
     4081 aagcaaatag tcttcctgta gtcaaatgtt attctgcctc gtgggatttt ttattaaatt
     4141 gcacgcgcta ttcctcttac ctaacaatcc taccaagacc tattactcat tttgctcata
     4201 tggacggttt agtggaggtc caactgaccg aacatccaaa atgggaagtt gttctagcct
     4261 ctctcaagca caataaaaca agccatttaa aacattatat caagcacacc atcttagatt
     4321 atttcacgaa tcagcgcttc taattgcttg actcagacct taaaaaaacg cctgaaggaa
     4381 acaatcccaa gcgtttcata tagttatttc ctaaaataca aataggtaaa tgatggcaat
     4441 acctgtgacc accaagacac aaggaatagt ataagccaca atttgattga attttagttt
     4501 gaagtttgag agataagata aggcccaaaa aggaactatc aagtttgttg tggcatctcc
     4561 taattgataa gcctgcaaaa tcttacctag gtcattacca acttcgatgg ttgcagggac
     4621 gatataggga gcttcaatca caaattttga gcctcctgat ggtaccgcaa tattcagcaa
     4681 agccgagtaa agataagcaa agaccggcca tgttgtccca tttgaaatag cgacaaagaa
     4741 atgggaaatc tctagtccta gacctgtact ttgaatgatt ccaaaaatac cagcgtaaaa
     4801 tggaaactga agcaccaagc cccaagatga catgacccca tctttgaata aggaaccata
     4861 atattctggt ccttgttgac cacaaagaag caaccctaag cctaaaaata aaaagttaaa
     4921 actattgaga tctagaccag acaagcccac caccgaaaaa tgataaaggc tatagctaac
     4981 aatggcccca ccaacaagat acatcactaa gcgagatgcg ttcatctttt cagcaggtgt
     5041 gtttcttgca ataacaacat gtgaagcggt atcaagacta tggtgataga tctcatcatc
     5101 aagttccatg attttagttt catcttttgg atgcgccaat aaagtaaagc aaagcggtac
     5161 cagcatacac accactaaaa gactgataaa tggaagattc aaaactgatt ctgtcagagg
     5221 aacactttca ggaacaacct gtttataagc atctgcgaca ctatttcgca aataatctgg
     5281 agtagccgaa taaagcaccg cagcaccaga taaaccagaa gtagctggta aaaaggtaaa
     5341 aaataaagta gcaacaaaca aaggtgtatg caccttaatc cctttttgcc tagcctgaac
     5401 aagcaactct ttcccaaaca caatggcaac catcatacca agaccccagt gtaaaaagcc
     5461 aaagatagag cctactacaa gagaaaaaat aaaaatctga gtcctggttt gaggcagttt
     5521 ggcaagggac ttaaacatct tatgagcagg tggagaactc gctacagcat tccctgtcgc
     5581 aagtaaaata gtcatctgca tggcaaagga caacagcccc caaaaaccat ttacccagcc
     5641 atagataatc ccgcccgtct tttccgtacc aataaaggga ctatccgtca tcaacacaga
     5701 catagtaacg actaaaaagg ttaaaataaa acagataaaa agcgattctg gcatccattt
     5761 cataaagcca tcaatatagc gatccataaa cctttttttg gttttgatgt tattcatgtt
     5821 gtcaattcct ttcacgttgt ccaaaagcac ttttgttctt ttgatcagta acctgtcgat
     5881 aatactaata agcgtttctt tagttcattt agttatcaat
//
LOCUS       DAUELZ010000093         5609 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.094, whole genome shotgun sequence.
ACCESSION   DAUELZ010000093 DAUELZ010000000
VERSION     DAUELZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.094"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..1779)
                     /locus_tag="VHI09_001410"
     CDS             complement(<1..1779)
                     /locus_tag="VHI09_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ6121278.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATNSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKERQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQTQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGFNIMDRIAPYFRPE"
     gene            2157..2840
                     /gene="rpiA"
                     /locus_tag="VHI09_001411"
     CDS             2157..2840
                     /gene="rpiA"
                     /locus_tag="VHI09_001411"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEQ6121279.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEGLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEAPKAG"
     gene            2917..4128
                     /locus_tag="VHI09_001412"
     CDS             2917..4128
                     /locus_tag="VHI09_001412"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEQ6121280.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     TAGLSVPNMAKIGLGNISRPIPLKTIPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIIANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDTA
                     MIGESFLGHLK"
     gene            4147..4587
                     /gene="arsC"
                     /locus_tag="VHI09_001413"
     CDS             4147..4587
                     /gene="arsC"
                     /locus_tag="VHI09_001413"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEQ6121281.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLTDPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYIKEDGEIVNPIEVKGEKDVIDD"
     gene            4571..5380
                     /locus_tag="VHI09_001414"
     CDS             4571..5380
                     /locus_tag="VHI09_001414"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ6121282.1"
                     /translation="MSLMTKINETKDFLVAKGIEAPEFGLILGSGLGELAEEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
                     VYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQHIKEDFKGLVKAILSEL"
BASE COUNT         1643 a         1050 c         1190 g         1726 t
ORIGIN      
        1 ctctggtctg aaataaggtg caatgcgatc cataatgtta aacccagaag tttccgtatc
       61 ttgttggcca aaaccggcat ttgatgtagc aataataacg gtatttttaa agttaatagt
      121 atttccttgt ccatctgtca atcgtccatc atctaacact tgtagaagaa gtgtaatgat
      181 ttgaggatca gctttttcaa tttcatctag taagacaata gcataagggt tacgacgaac
      241 acgctcagtt agggtattat tattatcatc gtagccaaca taaccagccg ttgtcccaat
      301 caattttgac acagcagtgc gatcgttata ttcggacata tcaagtcgaa taatagcatc
      361 tttggaacca aataaatcaa gtgctaattg tttagctaac tctgttttac caacaccagt
      421 tggacccaca aaaaggaaag aacctattgg acggttacca tcatcaaaac ctgctcgatt
      481 tcgtcgaata gcgcgtgcta ctgctgcaac agcctcatct tgtccgatga catggctttt
      541 taagcggctg ctaattcctt tgagcctttc taaatcatta gtccccatgt tagagacagg
      601 aataccagtt aagcgctcta cagactctgc gatatcattg actgtagcag tgacattttg
      661 ggtttggtta tgttgctcaa tttgcttttg taatgtttca atccgtgttt ttgctgctaa
      721 agctttttca aagtcttctt tggcgacagc tttttcttgc ctctcttttt gtttagctat
      781 ttcagtttcg agtgttttta agtcagtgac cgggtgttgc gcagctaaat gagcagctgt
      841 catatccagt aagtcaattg ctttatctgg caggctacgc tgtgggatgt attgaataga
      901 ataatcaaca gcagctttta gaacattatc aggaagggta atatggtggt gttgttcata
      961 aagattacga atacccatta aaatgtgaaa agtatcttca gctgatggtg cattcacttt
     1021 aacttcatta aagcgacgag cgagagcagc attttttaaa atagtattgc ggtattcgtc
     1081 ttgagtagta gcaccaatca gtgttaattc tcctcgtgac aaggctggtt taagaatatc
     1141 tgcaagtccc tttgagcctg aatcactgct tgtggcacca gcaccaacta tttgatgaat
     1201 ttcatcaaaa aataagatga tattcccagc ttcttttact tcttgaatca agttttgaat
     1261 ggtctcttca aaggaaccac gatactgtgt accagcttca agactagaaa tatcaattga
     1321 tacgatttcc ttgtttttaa tagcagctgg gacatctcca ttaacaatgg cttgagctaa
     1381 accttcaatg actgcagttt taccgacacc agcatctccg acaaggacag gattattttt
     1441 agttcgtcgt gccaagattt cagcggtatc ttgaatttct ttgttacgac cgatgacggg
     1501 atcaagatgc ccttgacgag cttcttgtgt taagttagtt ccgagttgag tcaatacact
     1561 atcagcttta ggtgttaaca tttgagaatt tgttgctttg gttgtcgcta cagggagttg
     1621 tcctgtttga cgatagtgtt ggaattcttc gggagttatt tcgcgaccgt taacgagata
     1681 gcggggattt tcgctacgat aaccacccat gtttgccatt aattggttga aaatatcatc
     1741 catatttaca aaaggatctt taccagaaaa atgtgtcata tcagttacct cgcaataaaa
     1801 aatagactat aagatgtttg taagcagtcg cagaatcgtt gttaggattt cacatgatag
     1861 gaaacccttt gctaataaaa tgattcagac attattttta ctcatcttat agtattatta
     1921 taatcaaaag gtcagtaaag gtcaaacaaa aacgcttgat tttcaaaaag ctaaataaac
     1981 acgaaaagta agtgcttata tgatgacttt agaataaatc ataagtttta aaaagatcat
     2041 cctattctat agcacttagg ctgtcaatga gagaaaaaca aatgttttca ttaacaagtt
     2101 ggcgctttca tggtataata aagtgctaag acgcaattct attataaggg ggaatcatgg
     2161 aagcactcaa aaaaattgcc ggtgttaccg ctgcgcaata tgtgactgat ggcatgacca
     2221 ttgggcttgg aactgggtca acggcttact attttgttga ggaaattggc cgtcgcgtaa
     2281 agcaagaagg actgcaagtg gttggtgtga ctacgtcaag tgtgactagc aagcaagcag
     2341 aaggcttagg cattccttta aaatcgatag atgatattga tagcattgac ctcaccgtag
     2401 atggtgctga tgaagtggat aaggatttca atggaatcaa gggtggcggc gcagccttac
     2461 taatggaaaa aattgttgca acaccaacaa aagaatacat ctgggttgtt gatgcttcta
     2521 aaatggtaga gcatctgggt gcctttaagt taccagtcga agtcgttcaa tatggtgctg
     2581 atcgtttgtt ccgtgtcttt gaaaaagctg gttacaaacc gtcttttcgt atgaaaggtg
     2641 atagtcgttt ggtgacagac atgcaaaact atattattga tcttgatttg ggttgtatta
     2701 aagatcctgt tgcttttggt cacctgctag atggaacagt tggtgttgtg gaacacggtc
     2761 tctttaatgg tatggttgat aaggtgattg ttgctagtaa agacggcgtt actgttttag
     2821 aagcgcctaa agcaggctag tcttagcata taacagtgtt aatatatttt ataggtattg
     2881 gggagtcaac cgtaacctcc cctagattgg agacttatgt caaaatttaa tcgtattcac
     2941 ttggttgttt tagactctgt cggtattggt gcagcgccag atgctgacaa attctttaat
     3001 gctggtgtag ctgatacaga ttctgacacc ctcggtcata tttcagaaac agcaggctta
     3061 tcggttccaa atatggcaaa aattggactt ggaaacattt ctcgtccgat accactaaaa
     3121 actattccaa cagaagacaa tcctactggt tacgtgacca aattggaaga agtatctctt
     3181 ggaaaagata ccatgacagg ccattgggaa attatgggct tgaacattac ggaacctttt
     3241 gacactttct ggaatggttt cccagaagag attttaacta agattgaaga attctcaggt
     3301 cgcaaaatta ttcgtgaagc taataaaccc tattcaggaa ctgcggttat tgatgatttt
     3361 ggtccgcgtc aaatggaaac aggtgagttg attgtttaca cctcagcaga cccagtgctt
     3421 caaattgcag cccatgaaga cattattcct gtggaagagc tttacaagat ttgtgaatac
     3481 gctcgctcca tcacgcttga acgccctgcc cttttaggcc gtattattgc gcgtccttat
     3541 gtaggcgaac caggaaactt tacacgtacg gctaaccgtc atgactatgc agtgtctcct
     3601 ttccaagata ccgttttgaa caaactggct gacgcaggtg tcccaactta tgctgttggt
     3661 aagattaatg acatctttaa tggttcaggt attacaaacg acatgggaca caataagtcg
     3721 aacagccatg gtattgacac gcttattaag actcttcaat tacctgagtt tactaaggga
     3781 ttctccttta ctaacctcgt tgattttgat gccaactttg gtcatcgtcg tgacccagag
     3841 ggttatcgcg actgcttgca tgaatttgac aatcgcctac ctgaaatcat cgcaaatatg
     3901 aaagaagacg atttgcttct catcactgct gaccatggta atgacccaac ctatgcaggc
     3961 accgaccaca ctcgtgaata cattccattg cttgcctact ctgcatcctt tactggaaat
     4021 ggtcttattc cacaagggca ttttgcagat atttcagcga ctgttgcaga aaattttgga
     4081 gttgatacag ccatgattgg ggagtcattc cttggtcact taaaataaga aagttagtaa
     4141 ttgataatgg aagaaatcac gatttatcat aatccaaact gtggcacttc acgtaatgtt
     4201 cttgccatga ttcgccatgc aggtattgag cctactatta ttgagtattt gcagacgcca
     4261 cctaatcggg agactttaat tgagctctta caatcgatgg gaatctctgc gcgagaactc
     4321 ttgcgaacca atgttcctga atttgaggct catggattag caaatcaagc agtagctgag
     4381 aaagacgtta ttaatgccat gttgacggat ccaattttga tcaatcgtcc tattgttgtg
     4441 actcgcaaag gagtaaaact ttgccgtcct tctgagacct tactggatat tcttcctgtt
     4501 cccctgccta gtccttatat taaagaggat ggcgagattg tgaaccctat tgaggtaaaa
     4561 ggagaaaaag atgtcattga tgactaaaat taacgaaaca aaagactttt tagttgctaa
     4621 aggaattgaa gctccagaat ttggattaat tctgggttca ggccttggtg aacttgctga
     4681 agaagtagaa aatgctattg ttattgatta tgcagatatt ccaaactggg gcaaatcaac
     4741 agttgttggc catgctggaa aattagtcta cggtgattta gctggtcgta aagtacttgc
     4801 cctacaaggt cgtttccatt tttacgaagg aaatccgctt gaagtggtga ctttccctgt
     4861 tcgtgtcatg aaagctcttg gatgtgaagg cgttcttgtc acaaatgcag ctggtggtat
     4921 cggctacggc ccaggtacct tgatggcaat cacagaccat atcaatatga ccggaaataa
     4981 cccactaatc ggtgaaaact tggacgagtt tggtccacgc ttccctgata tgtctgatgc
     5041 ctatactaag gtttatcgca ataaggcaca tgaagtggct gaaaaaatga acatcaagct
     5101 agaagatggt gtttatatgg gcttaactgg tccaacttat gaaacaccag cagaaatccg
     5161 tgctttcaaa gtattaggag cagatgctgt tggcatgtca actgtaccag aagttattgt
     5221 ggctgctcat tctggactta aagtacttgg tatttcagct attacaaact ttgcagctgg
     5281 ttttcaatca gaattgaacc atgaagaagt tgtggaagtg acccaacata tcaaagaaga
     5341 tttcaaaggt cttgttaaag ctattttatc ggagttgtag gacgaacaaa atcaactgga
     5401 gcattattac aggaatgtta ttttatgtcg tcagtttggc acatctgttc gcaataaaaa
     5461 aatcaaaaaa taatcacggg gtgtgagttc catgaatgtt gaactcagat tctagctttt
     5521 ctaactaaca taaccaacga agggaaagag acgtattcgg tttaaattga gtatggactc
     5581 gaacatttct tcgtattaat ttttaaata
//
LOCUS       DAUELZ010000094         5579 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.095, whole genome shotgun sequence.
ACCESSION   DAUELZ010000094 DAUELZ010000000
VERSION     DAUELZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.095"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(264..1406)
                     /locus_tag="VHI09_001415"
     CDS             complement(264..1406)
                     /locus_tag="VHI09_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEQ6121283.1"
                     /translation="MTINRVLKDDNLLQYTEKEFIEDMKILVAIDSFKGSVTSPELNT
                     SVAQALLSVDKQLVIETRAIADGGEGSLAALSQTVAGRWHQVKTIDLLRRPIKVAYYR
                     HAKQAFIESASIIGIDKITPNSVSYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTS
                     DGGKGFLESLNYDFMTGRSYLDTLASPVTLLGPTDVTNPYHGPQGFAAVFGPQKGGSL
                     SQIEETDQIASNFAKKVFCQTTIDLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAEL
                     LNLDNSLQSCDLVITGEGCLDTQSQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLA
                     AEDFLAVFSIQQQPISLEAAIDKTTTLSNIKILAANLMLLIAQFNK"
     gene            1687..4665
                     /locus_tag="VHI09_001416"
     CDS             1687..4665
                     /locus_tag="VHI09_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEQ6121284.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTTSAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVITTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRHLTNTFRNSRWYGFTGTPIFMENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLNSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLIQKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDKALSLYSNGGENDVLAPSWEEEKARFFEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAFQAFDKLFASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKESKAYEGKSIAYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGMSTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            4678..>5579
                     /locus_tag="VHI09_001417"
     CDS             4678..>5579
                     /locus_tag="VHI09_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ6121285.1"
                     /translation="MTKSKQPQYRFDGFEGEWEEKELGDIVQITMGQSPSSQNYTTNP
                     SDYILVQGNADIKNGYVFPRVWTTQITKQADKGDIILSVRAPVGDVGKTNYHVIIGRG
                     VAAIKGNEFIFQILKYLKEIGYWKRISTGSTFDSISSSNIKYAKIQIPSLPEQEAIGE
                     LFQTVDQLIQLQDQKLATLKEQKQTFLRKMFPAQGQKVPEIRLQGFDGEWEEKKLGEI
                     SRMFSGGTPSVGISEYYNGNIPFIRSSEINSDQTELFITNKGLSNSSAKIVEKNTLLY
                     ALYGATSGEVGLSRISGAINQAILA"
BASE COUNT         1846 a         1090 c         1018 g         1625 t
ORIGIN      
        1 acgtctccta agagacaact tctttattct atcaggtttc tcatagcctg tcaactactt
       61 tttcatcttt tttcaataag tgtgttacag gaacctctct gacagcttta ttagtatacg
      121 atatctttac tgtcattgtc aactcttttt tacaaaatgt ttctgttttt catactatgc
      181 taatgagtta gctcgtgcgt ctccttcttc ataggttact tttaaccgcc aaatgaatgc
      241 tacacaatcg aaaatagatg agtttactta ttaaactgag ctatcaatag cataaggtta
      301 gctgctaata ttttgatatt agaaagtgtt gttgttttat ctatcgctgc ttctaaggaa
      361 attggctgct gttgtattga aaaaacagcc aaaaagtctt ctgctgctag tcctgtctca
      421 atctttacag aaccacatag agcaatcgtt ggaacttgat attttttagc cattcttgca
      481 atcgcaacag gtaccttgcc agattgtgat tgtgtatcca ggcatccttc tccagtaata
      541 actaaatcac aagactgaag ggaattatcc agatttagta gctctgcaat cctgctaaaa
      601 ccactagtta aggtgcctcc taataggaca atggcacctc ctaatcctcc agctgctcca
      661 cttcctggta ttgtctgtaa atcaatggtt gtttggcaaa aaactttttt agcaaagtta
      721 cttgcaattt gatcagtttc ttcaatttga ctaagactcc cacctttttg agggccaaag
      781 accgcagcaa atccttgagg gccatgatat ggattggtca catcagtcgg ccctaatagc
      841 gtaacaggtg atgctaaagt atcaagataa gatcttcctg tcatgaaatc atagtttaaa
      901 ctttccaaaa accctttgcc accatcagaa gtgcctgttc ctcctagcat gatttcgatt
      961 tgagtagctc ctttttgaat ggcatcttta actgccaaac ccaaaccata ggaagtggct
     1021 tgtgcatagc ttactgaatt gggggtgatc ttatcaatac caataatact cgcagattca
     1081 ataaaagctt gctttgcgtg acgataataa gcgactttaa tcgggcgtct taatagatca
     1141 atggttttta cttgatgcca cctccccgcc acggtttgtg agagggctgc taaacttcct
     1201 tctcctccat cagcgatagc tcttgtttca ataactaact gcttatcgac agaaagaagg
     1261 gcttgagcta cactagtgtt aagttctgga gacgttaccg atcccttaaa agaatctatg
     1321 gctaccaaaa ttttcatatc ttcaataaat tccttttctg tatattgtag cagattatcg
     1381 tcctttaaaa ccctattaat agtcaagcta gatctgtctt aagtttttta tctattttga
     1441 gaactcgacc tttttactaa tgttgtctta ttactttctt caccttaaat tattgtcaag
     1501 caaactcaaa aatacaagaa ctttctcgat tgcatttaca tagtcatagc tttgtttagt
     1561 tgctaggatc ttaagcattc aactaatcaa aaaaagactg tttggaaggt ttcaatagct
     1621 ttgccattct aaaaaggatc cgccaatgat ataatattac tatatcatgt cgaatgggga
     1681 ttaaaaatgg gaaatcaaac taaaacggaa cttgaacttg aaaaagagtt gatccatctc
     1741 ttagaaacag gtgaaagtca atggacatat cgtaaagaat taaaaactga agatgcttta
     1801 tgggataatt tctttaagat cttagcgcaa aataacacgc aatatctgaa cgaggagcct
     1861 ttaacagcta gtgaaaaaga gcagattaag aatcaactta actttgtcaa ttactatgaa
     1921 gcggctaaat ggttagctgg tgaaaacggc attgctaaag tacaagtgca acgcgaagat
     1981 gcaaaactag gaacgattcg tttagaagtc gttaaagcag acaatgtggc tggtggaact
     2041 tctgtttatg agattgccaa tcaagttgct ttctctggtt ctcgtgatag acgaggtgat
     2101 gtgacacttc tcatcaatgg cttacctatg atccaaattg aattaaaatc tcagaatcat
     2161 caatgcattg aagcttttaa ccaagtcaaa aagtatgata aagaaggcca gtttagaggt
     2221 atcttttcaa ccttacaaat gtttgtggtc tcaaataaaa cagatacaag gtatatcgct
     2281 gcagctaaag aaaataaatt aaaccctaac ttcttgaccc aatgggtaga tcaaaacaac
     2341 aaaccacaaa aagatctctt tgcctttgcc aaagaagtcc tatctatccc tcgtgctcac
     2401 caaatggtca tgacttactc tgtcattgat gatgataaaa aagcactaat tctccttcgt
     2461 ccttatcaga ttcatgcaat cgaagccgtc gctgaagcga gtcgtcaccg caagtctggc
     2521 tatatctggc acacaacagg ttctggaaaa accttaactt cctataaagt ggctcgcaat
     2581 atccttcaaa taccggccgt tgaaaaatcc atctttgtca ttgaccgaaa ggatttagat
     2641 aatcagacca cttctgcttt tcaatcttat gcgcaaaatg atatctttga tgtcgatgag
     2701 acagaagata cgagacaact catcaaaaat ttagaatcca gtgatcgtcg tgttgtgatc
     2761 accaccattc aaaaactcaa tgctatgatc agtcagatgg aaagttatga cactccaaaa
     2821 ttcaagaaac taaaagagcg tcttgcacac ctaaatgtcg tctttgttgt agacgaatgc
     2881 caccgtgctg tcacgccaga acgtcaacgt catctcacga atacttttcg aaacagtcgc
     2941 tggtatggct ttacaggtac tcctatcttt atggaaaata agcgtgctca attaggtgat
     3001 ttagcccaaa caacggaaca gcaatacggc aaatgtctcc atcaatacac tgtcaaagaa
     3061 gccattcacg ataaagcggt tctaggcttc caagttgagt ataagacaac cattcctgat
     3121 atgcctgaag acagtattcc tgaagaagct tatgatcatg aagaacatat gttagctgtc
     3181 ttaaatagta tcattaatca atcacgtaag aagcttggct ttaataatgg gattggtcag
     3241 acattcgaag gcttattaac cgttaagagt attgctcgtg cccaagccta ttatgatctg
     3301 attcagaaag ttaaagctgg tgaaacggat ttagtcattt ctaagaaggt caaagaaaaa
     3361 ctaccagact ttccaaaggt agctattact tattctatca ctgaaaatga caatgcgtct
     3421 atcagccgac aagataaaat gaccaaaaat ctagaagact ataatcacct ctttggcact
     3481 aactttacca tcgataactt acaaggctat aacagagatt taaatgatcg cttagcccgt
     3541 aaaaaagata aattcaaaga ccgtcatgaa caattagacc tcgtgattgt ggttgaccgt
     3601 ctcttaacag gttttgatgc gccttgttta tcaacgatct ttattgatcg ccagcctatg
     3661 aaacctcagc acattattca agctttctca cgaaccaacc gtatctttga aagtagaaaa
     3721 cattacggtc aggttgtgac tttccaaaca ccacttcgtt ttaaagaagc tgttgacaaa
     3781 gcgcttagcc tctactctaa tgggggtgaa aacgacgttt tggccccttc ttgggaagaa
     3841 gaaaaagcaa gattctttga aaaagtaaca gtattaaaaa atatagttcc tgatccagac
     3901 gctttcccaa ctattgagag cgcacagaca gcttttctca aacaatacgc caaagccttt
     3961 caagcatttg ataaactctt tgcatctgta caagtctata gtgactttaa tgaaacactg
     4021 ttgagtgagg ttgggttatc agatgaagtg attgatactt ataaaggcac ttaccaaaat
     4081 gtcattgcag aaatacgaaa acgaagagaa gatgacgaag ccatcccaga gatcaatatc
     4141 gactatgagc tagaaagtgt tcagatggat gatatcaact atcactatat cttgacctta
     4201 attcaagcct ttgtcgatca agaacaagaa gctcttcaag aaaggctcaa tgacaatccc
     4261 atggatcaat acatccaaga tttggcaaaa tcaaaccctg ctatggctga ctcactagct
     4321 gagttatggc aagatatcca aaaagaatcc aaagcctatg aagggaaaag cattgcttat
     4381 gagttggata acctcattgg tgataagatc caaagggcca tcaaacactt tgcggaccaa
     4441 tggaaggcag atcctgacaa attagccttt gtggcgacta attatcatag tgctaactct
     4501 accaaacaag tgggcatgtc aaccttaaaa gaatccctag actaccaagc ctataaagaa
     4561 aaacaaggtg attctgccat gaataaactc aaatacaaga gccaatttga acgggagtta
     4621 gttcaattca ttagagatca aatccaaccc ttaaaagggc actaggaaag gaaagagatg
     4681 acaaaatcta aacagcctca atatcgcttt gacggttttg aaggggagtg ggaagaaaaa
     4741 gaactagggg atattgttca aattacaatg ggccaatctc catcaagtca aaattatact
     4801 acaaaccctt ctgattatat tctagtacaa ggaaatgccg atataaaaaa tggatatgtt
     4861 tttccgcgtg tctggacaac acaaattaca aaacaagctg acaaaggtga tattattctt
     4921 agtgttaggg ctccagttgg tgatgttgga aagacaaatt atcatgttat tattgggcgt
     4981 ggagttgctg ctataaaagg taatgaattt atctttcaaa tattaaaata cttgaaagaa
     5041 attggttatt ggaaaagaat tagtacaggt tcaacttttg atagcattag ctctagtaat
     5101 atcaaatatg ctaaaattca aattccctcc ctccccgaac aagaagctat tggcgaactt
     5161 ttccaaacgg ttgatcaatt gattcagttg caggatcaaa agctagcaac tctaaaagaa
     5221 caaaagcaaa ccttcctcag aaagatgttc ccagctcaag gacaaaaagt ccctgaaatc
     5281 cgtttacaag gttttgatgg tgagtgggaa gagaagaaat tgggagagat atctcggatg
     5341 ttttcaggtg gtactcctag cgtaggtatt tctgaatact ataacggtaa tattcctttc
     5401 attagatctt ctgagattaa tagtgatcaa acagagttat ttatcacaaa caaaggtcta
     5461 tcaaattcat cagcaaaaat agtggaaaaa aatacattat tatacgctct ctacggtgca
     5521 actagtggag aagttggact ttcaagaata agtggagcta ttaatcaagc aattttagc
//
LOCUS       DAUELZ010000095         5361 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.096, whole genome shotgun sequence.
ACCESSION   DAUELZ010000095 DAUELZ010000000
VERSION     DAUELZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.096"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            74..595
                     /locus_tag="VHI09_001418"
     CDS             74..595
                     /locus_tag="VHI09_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ6121286.1"
                     /translation="MTKDRQIKKTKTAIYSAFIALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFVETLKWWLHQRQRMS
                     ANELLKYYLELIK"
     gene            810..1328
                     /gene="rimM"
                     /locus_tag="VHI09_001419"
     CDS             810..1328
                     /gene="rimM"
                     /locus_tag="VHI09_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEQ6121287.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DRFVQEVTIVSHRKQKHFDIIKLKDMYHINAIEKYKGYTLKVSKDNQGDLQEGEFYYH
                     QIIGMAVYEKDRLIGYVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            1318..2037
                     /gene="trmD"
                     /locus_tag="VHI09_001420"
     CDS             1318..2037
                     /gene="trmD"
                     /locus_tag="VHI09_001420"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEQ6121288.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTIVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALG"
     gene            2049..3041
                     /locus_tag="VHI09_001421"
     CDS             2049..3041
                     /locus_tag="VHI09_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ6121289.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGVELTENLIKQLSRFEDRTTICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLKASTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGVFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            3219..4277
                     /locus_tag="VHI09_001422"
     CDS             3219..4277
                     /locus_tag="VHI09_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEQ6121290.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNKFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            4290..5213
                     /locus_tag="VHI09_001423"
     CDS             4290..5213
                     /locus_tag="VHI09_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEQ6121291.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     IVTGDVEETVKLPIMKSTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPNNQDAGHQV
                     ADLLNKANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
BASE COUNT         1701 a          854 c         1143 g         1663 t
ORIGIN      
        1 agttaaaata caattactat aaccaaaaat gagcacctgt tcaatagtga acaacattta
       61 gaaagtgtcc gatatgacaa aagacagaca aatcaaaaaa acaaaaacag ctatctatag
      121 tgcttttatt gctttattac aaaaaaaaga atattcgaaa ataactgttc gagatatgat
      181 tacacttgct aatgtaggga gatcaacctt ttatgcacat tatgaaagca aagaaatgtt
      241 actaaaagaa ctctgtgaag aactttttca tcatttgttt aggcaaaaaa gaaatgtgac
      301 ttttgaagac tatttggtac atattttaaa gcattttgaa caaaataaag acagcattgc
      361 gacgttgtta ctttctaatg atccctattt tttattaaga tttaaaaacg aactagaaca
      421 tgatgtttac cctaacttgc gctgcaagta tatcgacaaa acgactattc cagaagtatt
      481 cctgaaacaa tttgttctat ctagttttgt agaaacctta aaatggtggc tccatcaaag
      541 gcaaaggatg tctgcaaatg aactattaaa gtattattta gagctcataa aataatgact
      601 agtgagtcat cttgccaaat tcaatttttt aatacagacg tattttaaca atttaacaga
      661 gaaagacaaa actgtaagtg caaaatagac tataacgtga taaaataata ggtaatttgg
      721 ttctgaaaaa gaagtgtcta tcaaacttta gaatcaagag aggtcgctaa agtgacgtga
      781 gataaagagt taacgattta ggagctacca tggaatattt taatgttgga aaaattgtca
      841 acacgcaagg cctgcaagga gagatgcgcg tgttgtctgt cagtgatttt gctgaggaac
      901 gctttaaaaa aggctcacaa ttagctttat ttgatgataa agatcggttt gttcaagaag
      961 tgacaattgt tagtcaccgt aaacaaaagc attttgatat tatcaaattg aaagacatgt
     1021 atcatattaa tgctattgaa aagtacaagg gatacacttt aaaggtctca aaagacaatc
     1081 aaggagattt acaagaagga gaattttatt accatcagat tattggcatg gctgtttatg
     1141 aaaaggaccg gttgattggc tatgtcaagg aaatcttgca gccaggtgct aatgatgttt
     1201 gggtagtgaa acgccaagga aaacgagact tgctcttacc atatattccc ccagtagttc
     1261 ttaacgtaga tgttcctaac aagcgcgtgg atgttgaatt gatggaaggc ttagacgatg
     1321 aagattgata ttttaaccct ttttcctgaa atgtttgctc ccctagaaca ttccattgta
     1381 ggcaaggcaa aagaaaaagg tctgttagac attcactacc ataactttcg agattatgct
     1441 gaaaaagctc gtcatgtgga tgacgaaccc tatggtgggg gccaaggcat gttattgaga
     1501 gcgcagccca tttttgacac cattgaacag attgaagcca aaaaaccaag gattattctt
     1561 ttagatccag caggtaaacc ctttactcag gcttatgctg aagaattagc tcttgaggaa
     1621 gagttaattt ttatttgtgg tcattatgaa ggttatgatg agcggattaa gaccttggtg
     1681 acggatgaaa tttcattagg tgactttgta ttaacaggag gtgagttggc ggccatgact
     1741 atagtggatg cgaccgtcag gttaattcca caggtacttg ggaaagaaag tagccatcag
     1801 gacgattctt tttcatcagg acttttagaa taccctcaat acacaagacc ttatgattat
     1861 cgtggtatga cagttccaga tgttctaatg agtggtcatc acgaacgtat tcgcttgtgg
     1921 cgtctagaag aaagtctcag aaaaacctat ttgagaaggc cagatttgtt agagcggtac
     1981 gatttttccg aggaagagcg caagctattg gataagatta aagaagcatt gggttaagga
     2041 gaagattagt gaaagataaa gcatatgata ttacgattat tggtggtggt ccaataggtt
     2101 tatttgcagc cttttatgca ggtcttaggg gcgtaacagt aaagattatt gagagtttgt
     2161 cagaacttgg tggacagcct gccattcttt atcctgagaa aatgatttat gatattccag
     2221 cttatccatc tttaacaggt gttgagttga cagaaaattt aataaaacaa ttaagccgtt
     2281 ttgaagatcg tacaaccatt tgtttaaaag aagaagtatt aacctttgac aaggttaaag
     2341 gtggtttttc gattaggact aataaggcag agcatttttc aaaagccatt atcattgctt
     2401 gtggaaatgg cgcttttgca ccaagaactt tagggttaga aagtgaggaa aactttgctg
     2461 atcataacct tttttataat gttcaccagt tggaccagtt tgcgggacaa aaagttgtga
     2521 tttgtggtgg tggagattca gcagttgatt gggctttggc tttagaagat attgctgaga
     2581 gtgtgacggt tgtgcaccgt cgtgatgctt tcagagcaca tgaacacagt gtggaattat
     2641 tgaaagcatc aacggtcaac ctcttaacac cttatgtgcc aaaagcattg aaaggaattg
     2701 gaaacctggc tgaaaaatta gttattcaaa aagtgaaaga agatgaagtg ttagaacttg
     2761 aattggacag tcttattgtt agttttggtt tttcaacttc taataagaat ttgaaaaatt
     2821 ggaatctcga ctataagcgt tcaagtatta ctgtttcacc actttttcaa acaagtcaag
     2881 aaggtgtttt tgccattggt gatgcagccg cttataatgg gaaagtggat ttgattgcta
     2941 ctggttttgg cgaagcacca acagcagtta atcaggctat taactatatt tatccagatc
     3001 gagataaccg tgtggtgcat tcaacctctt taattgacta agggagtcgg aagtttcaag
     3061 agcagtagca acagctaata ttcagtttgt caggatagga caaatggttt ttacctaagt
     3121 cttcgtgaca agttttatac tttgtgataa aatagtcatt gtgaaaaaag tgtgatggtt
     3181 ttcacagatt aacacattac aatgaaaagg ggtctatcat gacaacaaca gataaagaga
     3241 catttagttc ttttatgaat aaggtcttgg ctggtacagc gattgctatc gttgtggcac
     3301 tcattccaaa tgctatttta gcgactttct taaaaccgct tttgccaaat atggcagctg
     3361 ctgaattttt acacattgtg caagtcttcc aattcttcac gccgattatg gctggtttct
     3421 tgattgggca acaatttaag tttaatccta tgcaacagtt ggctgttggt ggagctgctt
     3481 atatcggttc tggagcctgg gcttatacag aagtcattca aaaaggtgtg gcgacaggaa
     3541 cattccaact tagaggtatt ggggacttaa tcaatatgat gattacagct agccttgcgg
     3601 ttttagctgt gaaatatttt gggaataaat ttggctcttt aaccattatc ttgttaccta
     3661 tcactatagg aactggcgta ggttatattg gttggaaatt tttgccttat gtgtcttacg
     3721 tgacaaccct gatcggacaa gggattaatt cttttacaac cttacaacct attttaatgt
     3781 ctattttaat tgcagttgct ttctcgctta tcattgttag tccgatttca actgttgcaa
     3841 tcggtttagc tattgggctt aatggaatgg ctgcgggagc agcttctatg ggaattgcat
     3901 cgacagcagc agtcttagtg tgggcgacgc ttaaagttaa taaatctggt gtaccaattg
     3961 ctatcgcact tggggctatg aaaatgatga tgccaaactt cttgaaacat cctatcatgg
     4021 ctattccgat ggtgttcact gcagcaatta gctcattaac agtaccattg tttaatcttg
     4081 ttggaacacc agcttcatct ggttttggct tagttggtgc agtaggtccg attgcttctt
     4141 tagcaggtgg tagctcaata cttattatta tccttgcttg gatcattgtt ccgtttgcgg
     4201 ttgcttttgc ggcgcataag gtttctaaag atattctaaa actttataaa gaagacattt
     4261 tcgtctttga aggctaatag gaggaaaata tgttagttta tattgctggc tcaggagcca
     4321 tggggtgccg ttttggttac caaatttcaa aaacaaataa cgatgtgatt ttgttagata
     4381 actgggaaga tcatatcaat gccattaaag aaaacgggtt gattgtaaca ggtgatgtgg
     4441 aagaaacagt taagcttcct attatgaaat cgactgaagc cactcaagaa gccgatttga
     4501 ttatcttgtt tacaaaagca atgcagttgc cacaaatgct tcaagacatt aaaggcatta
     4561 ttggaaaaga aaccaaagtg ctttgccttt taaatggtct tggacacgaa gacgttattc
     4621 gtcagtacat tccagagcac aacattttga tgggggtcac tgtctggact gctggtctag
     4681 aaggaccagg tcgtgctcat cttcaaggag ttggtgctct taacttacaa agtatggatc
     4741 caaacaatca agatgcggga catcaagtgg ctgacttgct aaataaagct aacttaaatg
     4801 cgacttatga tgagaatgtt gtgccaaaca tctggcgaaa agcttgcgtg aatggtacca
     4861 tgaattcgac ttgtgctctt ttagattgta cgattggcga attatttgcc agtgaagatg
     4921 gtctcaaaat ggtaaaagaa atcatccatg agtttgttat cgttggtcaa gcagaaggtg
     4981 tcgaactaaa cgaagaagaa atcacacaat atgtaatgga tacatctgtt aaagctgctc
     5041 accattaccc atcaatgcac caagaccttg tgcaaaatca ccgattgact gaaattgatt
     5101 ttatcaatgg tgcagtcaat accaaaggtg aaaagcttgg tatcaatact ccatactgcc
     5161 gtatgattac agagctagtt cacgctaaag aagcagtgtt gaacatccaa taaacgctat
     5221 caaatcgtta cactcaatat tatggctaaa gctttaatat tgagtttttt gttatgctga
     5281 tagtattttt cttaggcatc attatttgtg agcctagatt atgagttaat aaaagaaata
     5341 ataattggta gaatttgata g
//
LOCUS       DAUELZ010000096         5274 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.097, whole genome shotgun sequence.
ACCESSION   DAUELZ010000096 DAUELZ010000000
VERSION     DAUELZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.097"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            214..537
                     /locus_tag="VHI09_001424"
     CDS             214..537
                     /locus_tag="VHI09_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121292.1"
                     /translation="MSKMKELNRLIHDMEETAKYYLRLVDEFKKLLTTEDETAPKSNP
                     PKNEPQKEIQLEDVRAVLATKAKDGYKEDVRALLNAYGASSLSVLDPKHFAAVLEEAG
                     GIGND"
     gene            530..1651
                     /locus_tag="VHI09_001425"
     CDS             530..1651
                     /locus_tag="VHI09_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEQ6121293.1"
                     /translation="MTNHAILSASASHRWLNCPPSARLTEDLPDTTSDFAIEGSDAHE
                     LCAYLVEKALGRNARDPTEDLTFYNDEMQECAEEYRNYVMEQVEKARGYSRDPTVLIE
                     QRLDFSKWVPEGFGTGDCIIVADGRLQVIDYKHGLGVQVDADHNPQMMCYALGALEMF
                     DGLYNFDNVTMTIFQPRKHNISTFKIEKTELLEWAENVLTPKAELAFKGEGEMQSGKH
                     CQFCKLKNVCRKRAEDNLALAKMEFADPASLDNEDIAEILPKLDLLISWANDIKAYAL
                     NQATDGHPIPGYKLVEGRSVRKFSDESAVSQAVIEAGYDPYEKKLLTITAMTKLLGKK
                     TFNDLLGGLIIKPSGKPTLVPIDDSRQEMNLAKNEFKEE"
     gene            1656..2204
                     /locus_tag="VHI09_001426"
     CDS             1656..2204
                     /locus_tag="VHI09_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEQ6121294.1"
                     /translation="MTTKVITGPNTRFSYLNANEPKSINGSTPKYSASLIIPKEDTAT
                     INKIKAAIEQAYKEGESKLKGNGKSVPALSTLKTPLRDGDLERPDDEAYKNAYFVNAN
                     SPHKPGVVDGNRQEIIDTSELYSGIYGRASITFYAFNSNGNKGIACGLNNLQKLRDGE
                     PLGGRTRAEDDFATEDDDDFLN"
     gene            2223..2390
                     /locus_tag="VHI09_001427"
     CDS             2223..2390
                     /locus_tag="VHI09_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121295.1"
                     /translation="MMYTILTCTIMGLWVLIGLYFGYMTIRDDIRNEVERKAKQNKEK
                     LSQTPLSRKNK"
     gene            2439..4394
                     /locus_tag="VHI09_001428"
     CDS             2439..4394
                     /locus_tag="VHI09_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEQ6121296.1"
                     /translation="MPIKELSIDIETYCEIDLRKSGVYRYAEDDSFELLLIAVSVDNG
                     PVTVYDFNKEKLPQDILEALVEDRVIKWAFNASFERICLSNWLKKHHPEFLSDGFLSP
                     VSWRCSMIWSAYLGLPLSLEGVGTVLKLKDQKMREGADLIRYFCLPCKPTKVNGGRIR
                     NFPHHAPNKWSTFIDYNRRDVEVELAIKERVKNFPVPSFVWNEYHQDQIINDRGIGID
                     VDFVKAALKIDGESKAKIQAELKTLTGLEKPNSVLQMISWLREHGVATDSLDKKAVKE
                     LLKTVDETTAQVLKLRQQAAKSSVSKYQAMMNCVCKDGRARGMFQFFGANRTGRWAGR
                     LVQLQNLPQNHLPDLKEARELFRTGDLESTELLYDTQDTLSQLIRTAFVPSKGKKFIV
                     CDFSAIEARVLSHLAGERWRSKVFEQGKDIYCMSASQMFGVPVEKHGQNSELRQKGKI
                     AELACGYGGSVGALKAMGALDMGLSEEELQPLVNSWRQANPNIVLYWWDVDNAVKTAV
                     KELLPTSTQNIQFEVKSGILFITLPSGRKLAYIKPRIGENQFGGESVTYEGTGTAKRW
                     ERLESYGPKFVENIVQAISRDILAFSMKQLNDFKIVGHVHDEVIIECPIEQKLDEVAS
                     LMGIAPDWLSDINLRADGYECFFYQKD"
     gene            complement(4433..5002)
                     /locus_tag="VHI09_001429"
     CDS             complement(4433..5002)
                     /locus_tag="VHI09_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121297.1"
                     /translation="MTINVNFFNYCQTQSSSTGVQPTESEVLAPMFVQDYYMDSRHSG
                     LLRGTFRQCRIMGIPILTAFVPVAEEDYEQMVWWYNNSVNDYLKDYRKPSKNAPKISS
                     WEAFTEKQDLPMIDDDIELYLFMDQFEFLKAKLAESNFQAPDILEMLFDGYENKEIFE
                     KLGVQKSAGYKKVKNTQKEGLELYNKFNK"
BASE COUNT         1698 a         1066 c         1081 g         1429 t
ORIGIN      
        1 aaacacctct tagatctact tagtaagatg taagggataa aaatattcct tacaaaacgg
       61 aaaaatcatt cgaaacctta cttagtaaga taggaggacc aaatatggta aacgaaccat
      121 acatcgaacc tgatgatgat gtagcagata ctctcatcgc catcagcgtc atctcaaaac
      181 tactcgctag aaaaattatg gaggaagaaa acaatgagca aaatgaaaga actgaataga
      241 ctgattcatg atatggaaga aaccgcaaag tactaccttc gcttggtgga tgagttcaag
      301 aaactcctca ctactgagga tgaaacagca cctaaatcaa atccaccaaa aaatgaacca
      361 caaaaggaaa ttcaattgga ggatgtccgt gctgtacttg ccacgaaagc aaaagatggc
      421 tataaggaag acgtccgtgc tcttctaaat gcttacggtg cttcttcact ctcagtactt
      481 gacccaaaac actttgcggc agtacttgaa gaagctggag gaattggtaa tgactaacca
      541 cgccattcta tccgcatctg catcacatcg ttggttgaat tgcccaccgt ctgctcgtct
      601 caccgaagat ctgccagaca caacatctga ttttgctatc gaaggttcgg atgctcacga
      661 gttgtgtgct tatctagtcg agaaagcctt gggtaggaat gcgcgtgatc caactgaaga
      721 tttaacattc tacaacgatg agatgcagga atgtgcagaa gaatatcgca actatgtcat
      781 ggaacaagtc gagaaagcta gaggttactc tcgtgaccct acagttctta tagaacaacg
      841 actggatttt tctaagtggg tacctgaagg ctttggaact ggagattgca tcatcgtggc
      901 agacggacgt cttcaggtta ttgattataa acatggactt ggtgttcagg ttgatgcaga
      961 ccacaaccct caaatgatgt gttatgcact tggagcactt gagatgtttg atggacttta
     1021 taatttcgat aacgttacca tgacaatctt tcaaccacga aaacataaca tttctacctt
     1081 taagatagaa aagactgagc tgcttgaatg ggctgaaaat gtactcactc caaaagctga
     1141 acttgcattc aagggtgagg gggagatgca atctggtaaa cactgccaat tctgtaaact
     1201 caagaatgtc tgtcgcaaac gtgctgagga taatttggca ctagccaaga tggagtttgc
     1261 ggatccagct tcccttgaca acgaggacat tgcagagatt ttgcctaaac tagacctgtt
     1321 gatttcatgg gcaaacgaca tcaaagctta tgctttaaat caagcaacag atggacatcc
     1381 tatcccagga tacaaactgg ttgaaggtcg ctctgttcgt aaattctcag atgagtcagc
     1441 cgttagccaa gcagtgattg aagcaggcta tgacccttat gagaagaaac tgctcactat
     1501 cactgccatg actaagttac ttggcaagaa aacttttaat gacctgcttg gtggtcttat
     1561 cataaaacca agtggaaaac cgacactcgt tcctattgac gatagccgtc aagagatgaa
     1621 cctagcaaaa aatgaattta aagaggaata actatatgac aaccaaagta ataacaggac
     1681 caaacactcg cttcagctac ttaaatgcca acgagccaaa atctataaat ggtagcactc
     1741 ccaagtacag tgcctcactc atcatcccaa aagaggatac tgccaccatt aacaaaatca
     1801 aggctgctat tgagcaagca tataaagaag gtgagtcaaa gctcaaaggc aatggcaaat
     1861 cagtacctgc attatctact ctgaaaactc ccctacgaga tggtgacctt gaacgccctg
     1921 atgatgaagc atacaaaaat gcctacttcg taaatgccaa ctcgccacac aaacctggtg
     1981 tggttgatgg caaccgccaa gaaattatcg atacgtctga gctatactca ggtatctatg
     2041 gtcgtgcttc tatcaccttc tacgcattta attccaatgg caataaaggg attgcttgcg
     2101 gtttgaataa cttgcaaaaa ttgcgtgatg gtgaacccct cggaggacgc actcgtgccg
     2161 aagatgattt tgcgacagaa gacgatgatg actttttgaa ctagaaatgg agaatttggt
     2221 agatgatgta tacgatttta acttgtacaa ttatgggtct ctgggtgctt atcggtctat
     2281 acttcgggta tatgaccatt agagatgaca ttcgaaatga agtggaacga aaggcaaagc
     2341 aaaacaaaga aaaacttagc caaacaccac tcagtcgaaa aaacaaatag aactttaggt
     2401 ggcagtactt ctgtcacctt tttcagaaag gacaaactat gccgattaaa gaacttagca
     2461 tcgacattga aacctattgc gaaattgacc tacgaaaatc tggtgtctat cgctatgcgg
     2521 aagatgacag ttttgaactc cttttaattg cagtttctgt cgataatgga ccagtgactg
     2581 tttacgactt taataaagag aagttaccac aagatattct tgaagcttta gtagaagata
     2641 gagtcatcaa atgggcattc aacgcttcat ttgagcgaat ttgcctatcc aactggctca
     2701 agaaacatca tcctgaattc ttatcagatg gatttctctc cccagtttcg tggcgatgta
     2761 gcatgatttg gtccgcctat ttaggacttc cactctccct tgaaggagtt ggaacagttc
     2821 tcaaactcaa agaccaaaag atgagagagg gggctgacct tattcgctac ttctgcttgc
     2881 cttgtaagcc aaccaaagtc aatgggggac gaattcgtaa ctttcctcat cacgcaccta
     2941 acaagtggtc tacatttatc gactacaaca gacgtgatgt tgaggtcgaa ttggccatca
     3001 aggaacgggt gaaaaacttc ccagtaccta gctttgtttg gaatgagtac caccaggatc
     3061 agattatcaa cgaccgcggt attggcatag atgttgactt tgtaaaagct gctttaaaaa
     3121 tagacggaga gagcaaagcc aaaattcaag cagagttaaa aacattaacg ggtcttgaaa
     3181 aacccaactc tgttctgcag atgattagtt ggttgcgaga acacggagta gcaacagatt
     3241 ctctagacaa aaaagctgtg aaagaattac tcaaaacggt tgatgaaaca actgctcaag
     3301 ttctcaaact tcgtcagcaa gctgccaaat caagtgtctc caaataccag gctatgatga
     3361 actgtgtttg taaagacggt cgagcaagag gaatgtttca atttttcggg gctaatcgta
     3421 caggtcgttg ggctggtcgc ttggtacaac ttcagaattt accacagaac catcttcctg
     3481 acctaaagga agctagagaa cttttcagaa caggtgactt agaatctact gagctactct
     3541 acgacacaca agatacctta tctcaactta tccgaacagc ctttgtccct agtaaaggaa
     3601 agaaattcat tgtttgcgac ttttcagcta tcgaagctcg tgtactctcc cacctggcag
     3661 gagagagatg gcgcagtaaa gtatttgaac aagggaaaga catctactgt atgtccgctt
     3721 ctcagatgtt tggagtacca gttgaaaaac atggacaaaa ttctgaactc aggcaaaaag
     3781 gaaaaattgc agagcttgct tgcggctatg gtggttcagt cggtgcattg aaagccatgg
     3841 gagcacttga tatgggacta tcagaggaag aactccaacc acttgttaac tcatggcgac
     3901 aagcaaatcc gaatatcgtt ctctattggt gggatgtcga taatgctgta aagactgctg
     3961 taaaggaact acttccaaca tctactcaaa atattcaatt tgaagtcaaa agtggtatat
     4021 tgttcatcac ccttccttct ggtcgcaaac tagcgtatat caaaccaaga attggcgaga
     4081 accagttcgg tggagagtcc gtcacttacg aaggaactgg aactgccaaa cgttgggaga
     4141 ggttagaaag ctacggtcca aaatttgtag agaacattgt tcaagctatc agtagagaca
     4201 tacttgcgtt ctctatgaag caactaaatg atttcaagat tgtaggccat gtccacgatg
     4261 aagtaataat cgaatgtcca attgaacaaa aacttgatga agttgcatca ttaatgggga
     4321 tagcacctga ctggttgtct gatattaatc ttagggctga tggatatgaa tgctttttct
     4381 atcaaaaaga ctagcaaaaa atcgccacct cgtaattgag atggcgattc tgttatttat
     4441 taaatttgtt gtaaagctct aacccttctt tctgagtatt ttttactttc ttatatccag
     4501 cagatttctg tactcctagt ttttcaaaga tttccttatt ctcgtaacca tcaaataaca
     4561 tttccaaaat atctggtgct tgaaaatttg attctgcaag ttttgctttc aaaaactcaa
     4621 actgatccat gaatagataa agctcaatgt catcatctat cattggtaaa tcttgtttct
     4681 ctgtgaatgc ttcccaagat gaaatcttag gagcattttt acttggctta cgataatctt
     4741 taagataatc attaactgag ttattgtacc accagaccat ctgttcatag tcctcttctg
     4801 cgactggaac aaaggcagtt agtattggaa tacccataat acgacattga cgaaacgtac
     4861 cacgtagcaa acctgaatgg cgtgaatcca tgtaataatc ctgcacgaac atgggtgcta
     4921 gtacttcact ttcagttggt tgtactccag ttgatgaaga ttgagtttgg cagtagttga
     4981 aaaagttgac attgattgtc atgataaatt ggcttcccag ttaataccga aagcacacat
     5041 gacaaatcaa ggcatgcaaa aaatattctt gaccgcatag ttactttcct ctatgttatc
     5101 ggtcagccaa tttcacaagc tgaaaaaagt aatcaatacc aaaacatctg aaattatggt
     5161 cgtcgtcacg actccgtttt tgtaatgata atcgtttaga gatatctcat ctcttagtta
     5221 aattgtaata gaaaataatc cgtaattgaa gttcacgatt aaaacgatta ctat
//
LOCUS       DAUELZ010000097         5249 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.098, whole genome shotgun sequence.
ACCESSION   DAUELZ010000097 DAUELZ010000000
VERSION     DAUELZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.098"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            73..786
                     /locus_tag="VHI09_001430"
     CDS             73..786
                     /locus_tag="VHI09_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ6121298.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKAIAQRASQLIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGVDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            783..1694
                     /gene="pfkB"
                     /locus_tag="VHI09_001431"
     CDS             783..1694
                     /gene="pfkB"
                     /locus_tag="VHI09_001431"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEQ6121299.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQVTRINVKIKSQEETELN
                     GQGPIISQEQLEELKTKLSQLTAEDTVVFAGSAPANLGNAVYKELLPLVRQSGAQVVC
                     DFEGQTLIDALTYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNAIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            1691..3637
                     /locus_tag="VHI09_001432"
     CDS             1691..3637
                     /locus_tag="VHI09_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ6121300.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKTVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSET
                     AVSTEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAVFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAALPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            3730..4329
                     /locus_tag="VHI09_001433"
     CDS             3730..4329
                     /locus_tag="VHI09_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ6121301.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDATFRSYGSLTACFLDYARVLNDPLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYHTKLNELIEWYQLTNFDGLMK"
     gene            4481..5188
                     /locus_tag="VHI09_001434"
     CDS             4481..5188
                     /locus_tag="VHI09_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ6121302.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYHNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
BASE COUNT         1587 a          892 c         1142 g         1628 t
ORIGIN      
        1 atagtataat aattgtaaac gatttcagtt aggggtggtt ctatcttaaa atcaaaacga
       61 aaacaattaa tcatggcaaa gatcactgaa gaaaactatg tttctttaga ggatttaatg
      121 caattactca actcctctga gtctactatc cgtagagatc taggagagtt ggagcaagaa
      181 ggacggctac atcgtgttca tggaggtgct gaactttttc actctttaca agaggaactt
      241 tcgaaccaag aaaagtctgt caaaaacagt catattaaaa aagctattgc gcaaagagcc
      301 tctcagctta tctatgacaa cgatgtgatc tttattgatg ctgggacaac gactgaattt
      361 ttattacctt ttttacaagc taaaaattta actgtagtta ctaattctat tcatcatgct
      421 gctagattag ttgaactctc tattgagacg attattgtag ggggttatgt caaacaaacg
      481 accgatgcta gtattggtaa tgtagctttg gaacagatta ggcagatgaa ttttgacaag
      541 gcatttcttg gaatgaatgg tgttgatgat tcttacctga cgactcctga tatggaagaa
      601 gcagttatta aaaaagcggt tctgtcaaat gcaaaattag cttacatttt agtagatggc
      661 actaaaattg gtcaggtatc gtttgtaaaa gtagctccta ttaatgatgt gactattatt
      721 acattaggag gatcagcaag tattcttaaa caaataaagg aaaaggcaaa ggtgattgaa
      781 ttatgattta taccgtgacc ttaaaccctt ctattgactt tattgtcaga attgaccaaa
      841 taaatcttgg gtcagtgaat cgcatggcga gtgatgataa gtttgctgga ggtaaaggca
      901 ttaatgtgag tcgcatcttg caacgtttag acattgctag cacggcgaca gggttcttgg
      961 gtggttttac aggacgtttt atcgaagaaa gtttgagtgc agaaggcgtt aaaactgatt
     1021 ttgtaaaagg tgatcaagtt actcgaatca atgttaaaat caagtcacaa gaagagaccg
     1081 aattaaatgg tcaaggacct atcattagtc aggaacaact agaggagttg aaaactaagc
     1141 tttcccagct aacagcagag gatacagttg tctttgcagg ttcagcgcca gctaatcttg
     1201 gcaatgctgt ttataaggaa ttacttcctt tagttaggca aagcggtgct caggtggttt
     1261 gtgattttga aggacagact ttaatagatg cgcttacata taatcctctg ttggttaaac
     1321 ctaataatca tgaactagaa gctatttttg gtacgattct gactagtctt gatgatgttg
     1381 aaacctatgc ccgtcgtctt ttagagatgg gtgctcaaaa tgccatcatt tcaatggctg
     1441 gtgatggtgc tttgctggtt actaaagaag cgacttattt tgctaaaccg attaagggag
     1501 aagttaaaaa ttctgtcggt gctggtgatt cgatggttgc tggctttaca ggcgaattta
     1561 tgaagagtca aaatccaata gaagccttaa aatggggcgt ggcttgtggt acagcgacgg
     1621 ctttctctga tgacttggcg acaattgcat ttattaagga aacatatcat aaagttgagg
     1681 tagaaaaacg atgaaaattc aagatttatt gagaaaagat attatgattc tcgatttaca
     1741 ggctatttct aaagaagttg ccattgacga gatgattact aaattagttg aaaaagatat
     1801 tgtacatgat tttgatgtct ttaaaaagag tatcatgaca cgtgaagaac aaacatcaac
     1861 agggcttggt gatgggattg ccatgcctca ttctaaaaat attgtagtag ataaaccagc
     1921 ggtcttgttt gcgaaatcaa ataaaggcgt ggattataag gctttagatg gtcaaccaac
     1981 ggatctcttc tttatgattg ctgcgcctca aggagcaaat gatactcact tggctgcgct
     2041 agcagaatta tcacaatacc ttttgaaaga cggctttgct gataaacttc gtgctgcagc
     2101 gacaccagaa gcagtcattg ctgtttttga tgaagcgtca acagctaaag aagaagtggt
     2161 tgctccaaca agtggtcaag actttatcgt tgctgttaca gcttgtccga caggaattgc
     2221 tcacacttat atggcagaag aggctttgaa aaaacaagct gcagaaatgg gagtagctat
     2281 taaagttgaa acaaacggtg cttctggtgt ggctaatcgt ttaaccgctg aagatattca
     2341 gagagctaaa ggagttattg ttgcagctga taaaacggtt gaaatggacc gttttgatgg
     2401 taaacaattc attgctcgcc ctgttgcaga tggtatcaag aaaagtcagg aattgatttc
     2461 tttgatttta aacaatgaag gaaacactta tcatgctaaa aatggaaaat ctgaaacagc
     2521 agtatcaact gagaaaacaa gtttaggcgg tgctttctat aaacacttga tgggcggtgt
     2581 ctcacaaatg ttgccatttg ttattggtgg cgggattatg attgctctag catttttatt
     2641 ggataacatg cttggtgtgc caaatgatca gcttggaagt cttggatcct atcatgaaat
     2701 agcagctatt tttatgaaca ttggtggagc agtcttttcc ttcatgttac cagtactagc
     2761 aggttatatc gcttattcta ttgctgaaaa accgggctta gtagctggtt ttgtggcagg
     2821 tgccattgcc tcaaatggtc ttgcttttgg taaggtacca tttgcagcag gaggagaagt
     2881 gagcttgggc ttaacaggcg tgccatcagg tttccttggg gcgcttgtgg gtggtttcct
     2941 tgccggtggt gttattcttg cccttcgtaa attgttggca gccctaccac gttctctaga
     3001 aggggttaaa tctatcctcc tttacccatt acttggcgtc cttgtcactg gtttcttgat
     3061 gctttttgtc aatattccaa tggcagctat taacacagct cttaatgatt tcttacaagg
     3121 tctttcagga agctctgctg tccttatggg acttcttgtt ggtggaatga tggctgttga
     3181 catgggtggt cctgttaata aggcagctta tgtttttggt acaggaactt tagcagctac
     3241 tgtggcaaat ggtggttcgg tcgttatggc cgctgttatg gcaggtggta tggtacctcc
     3301 tcttgctgtt tttgtagcca ctctcttgtt taaagataaa tttaccaaag aagaacgtga
     3361 atcaggcttg acaaatattg ttatgggact ttcattcatc accgaaggag caattccgtt
     3421 tggtgcagct gacccagcac gtgcgattcc tagctttatc gcaggttctg ccttgacagg
     3481 agctcttgtt ggtttggctg gtattaaatt aatggcgcca catggtggta tcttcgtaat
     3541 tgctttaaca agcaatccaa tcttatatct tgtctttgtt gtgattggtg cccttgtatc
     3601 aggtatctta tttggcgctc ttcgcaaaaa agcctaaaag gtaaagaact tttttcttac
     3661 taaaactaga atctgtcaag gttctagttt tttgctatac tagagttaaa ggataagaaa
     3721 tgaggacctc tgaaggtgaa aaaaaggaga agaagggcta aatcatccgt aaatcgactt
     3781 gtcttagggt tagtattact taatctgatt gttagtatgt ggacattaaa attgggaaac
     3841 cagcgattag ctccgtatgc ggatcacgaa actctgacat ttgtcaggaa gattagtcat
     3901 gctgctcaat cagtcgctca aaagaaacag ttatacagtt cggtgatgat ggctcaggcc
     3961 attttagaat ccaataatgg caagtcacaa ctaagtcaaa aaccttatta taattttttc
     4021 gggattaagg ggagctataa agaacggtca gtcatttttc caactttaga agacgatgga
     4081 caagggaatc tttatcaaat tgatgctact tttcgttcct atgggagcct gacagcttgt
     4141 tttttagatt acgcaagagt tttgaatgat ccactttatg acaaaaccca caaaaagttt
     4201 tggtctcatt atcaagatgc tactgcaacc ttaacaggca cttacgccac tgatacaact
     4261 taccatacca aattaaatga attgattgaa tggtatcaac tcactaattt tgatggtcta
     4321 atgaaatagt tatccacaat tttaaaaaaa atcagaagaa atatgtcgat ttcaagacaa
     4381 aagttatcaa tctcattaaa ttatctgtgg aaaagttaat ttaaacgttc gtgtgctata
     4441 atagaaacat agaaaatgta taaaaaagga tatgtgaata atgacaaaaa agaaaggtaa
     4501 gcttgtactt atcagtcttt ttgttctagc agcttgttta ggagcttata gtgcaatgag
     4561 acaatctcat aaaacttcaa acgtgtcagc tgagactatc gccagttctt caacccgaca
     4621 ttttattgat gaaattggtc caactgctag tactattggt caagaacgtg atctttatgc
     4681 gtctgttatg atagcacaag cgattttaga atcaagtaat ggtaaatcaa gtctaagtca
     4741 agctccctat cataacttct ttggcatcaa aggggcctat aatggttcgt cagttaccat
     4801 gtcaacttgg gaagatgatg gtaatggcaa tacctatacg attgatcaag cctttagagc
     4861 ttatccaagt attgcagact cccttaatga ctatgcggac ttgctaagtt ctagcactta
     4921 tataggtgct agaaaatcaa acacgctatc gtatcaagat gctacagcag ctctaactgg
     4981 cttatacgct acagacacca gttataactt aaaactaaat aatattattg caacctatgg
     5041 tttgacggct tatgatgttg ctaatagctc agctcaagaa actggtctag caacttcggg
     5101 ttatgtttgg aatgagtatc gtcgtaatta cactgatgcg gagaccctag cagttgatga
     5161 ggcttgggct aaacgcatga cttattaatg taacactaac aacaatctgc ttgcactgac
     5221 cccaaaaaag ctggatactc tattttaag
//
LOCUS       DAUELZ010000098         5213 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.099, whole genome shotgun sequence.
ACCESSION   DAUELZ010000098 DAUELZ010000000
VERSION     DAUELZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.099"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..784
                     /locus_tag="VHI09_001435"
     CDS             <1..784
                     /locus_tag="VHI09_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ6121303.1"
                     /translation="KALDITVVPLSVMIDSKLYSDNDLKEEGHFLSLMKASKSLPKTS
                     QPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTIEASRQGAEIAEAPVTVLDSGFTD
                     QAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTELYIGVSTLENLVKGGRIGRVTGV
                     LSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLDSYLAKNSHRPIAEIAISYAGEAS
                     LALTLKERIAAYYNHSISVLETGSIIQTHTGEGAFAVMVRYE"
     gene            777..1619
                     /locus_tag="VHI09_001436"
     CDS             777..1619
                     /locus_tag="VHI09_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEQ6121304.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLKKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            1597..2184
                     /locus_tag="VHI09_001437"
     CDS             1597..2184
                     /locus_tag="VHI09_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEQ6121305.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            2283..2558
                     /locus_tag="VHI09_001438"
     CDS             2283..2558
                     /locus_tag="VHI09_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEQ6121306.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(2647..3789)
                     /locus_tag="VHI09_001439"
     CDS             complement(2647..3789)
                     /locus_tag="VHI09_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ6121307.1"
                     /translation="MWIEETDNGKFKFRENYKDPYTGTWKPVSVTMEKDNSRAYKAAR
                     KILEQKITEKIAQLKASELLFTELLDEWWAFYKKELKRSSVASLRGNIEEIRETFGIG
                     VKVVNIDPKYVQNYLDNLDCSRNKKERNKSMLNLAFDYAVGLDIIQDNPARRAKLPRV
                     KKTLEDWKKAEEKYLEEDEIKPLLKELYRRPSTYRLGLLAEFISLNGCRIGEAVSIEP
                     CNYESKSRILQLHGTFDHTEGYRNGEKTAPKTLASYRETIMTSRELEILQELEFMNEL
                     EKNTNHRYRDMGYLFTTKNGVPIQTNSFNLALKKANERLEDPITKKLTSHIFRHTLIS
                     RLAENNVPLKSIMERVGHADAKTTAQIYTHVTKKMKSSVADIMENY"
     gene            complement(3913..4431)
                     /locus_tag="VHI09_001440"
     CDS             complement(3913..4431)
                     /locus_tag="VHI09_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEQ6121308.1"
                     /translation="MAKVIFQDNFLLMGTNYHEKEANKVMAEIGKKSPYWDKDKDFIS
                     DYIKSNFKDIYKYYRVSTKDVEVVREPLNRHDPNAIKVMVNKTFVGYFPADLAKRLTP
                     YVKKSSHYQMEATLTGRGGQYKTLKNDLKTVVTKKKDITYKLRLTILKVDRVSKSKNA
                     GLLESIASWFLN"
     gene            complement(4443..5198)
                     /locus_tag="VHI09_001441"
     CDS             complement(4443..5198)
                     /locus_tag="VHI09_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6121309.1"
                     /translation="MEQLGDRIRKLREGRNMTQTELSEILGMKTYTTVSKWEKNENFP
                     KGKDLKKLAEIFNVTSDYLLGLTDSKLGKITIQNEQPEIVSIYNQLEQPRQEKVLNFA
                     NEQLEEQNKTVSIFDKKSEETEDYITDYVEGLVAAGLGAYQEDNLHMKVKLRSDDVPD
                     EYDTIAKVAGDSMEPLIQDNDLLFIKVSSQVDMNDIGIFQVNGKNFVKKLKRDYDGAW
                     YLQSLNKSYEEIYLSKDDDIRTIGEVVDIYREY"
BASE COUNT         1549 a          911 c          937 g         1816 t
ORIGIN      
        1 taaagcttta gatattactg tagtaccttt atctgtcatg attgatagca aattatattc
       61 tgataatgat ttaaaagaag aaggtcattt tttgagcctt atgaaggcta gcaaatccct
      121 tcccaaaacc agtcagccac cggttggact ctttgctgaa acttacgaaa atttggtcaa
      181 aaaaggagta actgacattg ttgctattca cctctcacca gctttatcag gtactattga
      241 agcttcccgt cagggggctg aaatcgctga ggcacctgtt acagttttag attcaggatt
      301 tactgatcaa gccatgaaat ttcaggtcgt tgaggcagca aagatggcaa aagcgggtgc
      361 aagtttgaat gagattttgg ctgctgttca agctatcaaa tcaaaaacag agctctacat
      421 tggtgtatct accttagaaa atttagtaaa aggaggtcgt attggtcgtg tgacgggtgt
      481 tttgagctcg ctacttaatg tcaaagttgt tatggcactt aaaaatgatg agttaaaaac
      541 gcttgtcaaa ggtcgaggta ataaaacatt tacgaaatgg ttagatagtt acctcgctaa
      601 aaatagtcat cgcccgattg cagagatagc tatttcttat gctggtgaag ctagcctggc
      661 attaacctta aaagaaagaa tcgcagctta ttataaccat tctatttcgg ttttagaaac
      721 aggctctatc atccaaacac atacaggaga aggagctttt gcggttatgg ttcgttatga
      781 ataatcgtca tttatttagt gggatatttt tctttgttat tagtttatgt ctggcctttt
      841 tattgttaaa tattattatc cctaagtcaa attcacgttt gaaaaagagt gattttctga
      901 aaaaagaaca agtagctatc caatatgttg ctataggaga ttcattgaca gaaggagtag
      961 gtgatctaac tcatcaaggt ggttttgttc ctttgttaac gaatgatctc agtgaatatt
     1021 ttaaggctaa tgttaatcat caaaattacg gcgtatctgg tgataccagt caacaaattc
     1081 ttgataggat gataaaacaa aagcagatac agttatcttt aaaaaaagca gatataatga
     1141 cgttaaccgt tggtggtaat gatgttatgg cagttattcg gaagaattta gcggatttgc
     1201 aagtttctag ttttagaaag ccagctcgtc agtatcaaaa acgattaaga cagattatcg
     1261 agttagccag aaaagataat aaagatcttc ctatttttat tttaggcatc tataatccgt
     1321 tttatttgaa ttttccagaa ctaactgata tgcaaaaagt gattgatgac tggaatacca
     1381 aaactaagga ggttgttgga gaatacgatc gtgtgtactt tgtgccaata aatgacctct
     1441 tgtataaagg gataaatgga caagaaggaa ttgtccattc ttcaggagat caaactacaa
     1501 ttgtcaatga tgccttgttt actggggacc attttcaccc aaataatact ggctatcaaa
     1561 tcatgtcaaa tgcagtaatg gagaaaataa aaaagcatga aaaaaaaatc aaaccttaat
     1621 tggtggaagt ggagcttctt atgtctgctt gcttttaata ccgccttttt gatggttatt
     1681 gcaagtcgtc tcattcaagt aagagaacct gagtcagaac tgattgctaa aaaaccagtt
     1741 aaaaatatca aaattggtac atttgtcaca accagagaac agcttaacga aactgtagca
     1801 agttatctta aagattatca gactaaaaaa atgtcttata aattttatgc gacatcttct
     1861 tctattttgt ttgaaggaac ttatcaatta ttagggtatg aagtcccttt atatatctat
     1921 tttcaacctc atcgtctaga aaatggagct gtccaactac aggtgatctc tttttcagtg
     1981 ggaactctgc cactcccaga aaaagatgtt ttgcagtatt tgaaatcaag ctacaaattg
     2041 ccaagctttg ttaaagtgat gccaaatcaa tcagctatcg ttgttaattt acaagatatc
     2101 caaaatgatg ctaaggttta tttaaaggcc aagaaaattg atttatttaa tgatgaaatc
     2161 agttttaaca tctataagaa ataagctcaa ataacgttaa atacttgacc taatcgcttt
     2221 ttttagatat gataatatct gttaaagcat taagcttact aattatttgg aggatttgtt
     2281 aaatggctaa caaacaagat ttaatcgcaa aggttgcaga agcaactgaa ttgactaaaa
     2341 aagattcagc agcagcagtt gatgccgtgt tttctacaat cgaagctttc cttgctgaag
     2401 gtgaaaaagt acaattgatc ggttttggta acttcgaagt acgcgaacgt gcagctcgta
     2461 aaggtcgtaa cccacaaact ggtgcagaaa ttgaaattgc agcttcaaaa gttccagcct
     2521 tcaaagctgg taaagctctt aaagacgctg ttaaataatt gcatatataa aagcccattg
     2581 tacaagcgtt gtagcctgtg cagtgggctt ttttgtgttc aggggggtaa aaaaggggta
     2641 aaaaatttaa tagttttcca taatatcagc tacacttgat ttcattttct ttgtgacatg
     2701 agtataaatt tgggcagtag ttttagcgtc tgcatgtcct acacgttcca tgattgattt
     2761 tagcggcacg ttattctctg ctagacggct aataagagtg tgacggaaga tgtggctagt
     2821 aagttttttt gtaattgggt cttccaatct ttcattagct ttttttaaag ctaagttgaa
     2881 tgagttagtc tgaattggaa caccgttttt tgttgtaaaa aggtatccca tatctctata
     2941 tctatgattt gtattttttt ctagttcgtt cataaattct aactcttgta gaatttcaag
     3001 ttctctactt gtcatgatgg tttcacgata agaagctaat gtctttggtg ctgtcttttc
     3061 cccgttacga tatccttctg tatgatcaaa tgttccgtgc aactgcaata ttcttgactt
     3121 agattcgtag ttgcacggct caatgctgac agcttcaccg atacgacaac cgttcaaact
     3181 gataaactca gccaacaaac caagtcggta agtgctaggt cttctgtaca attctttcaa
     3241 taatggttta atttcatctt cttcaagata tttttcttca gcctttttcc agtcttcaag
     3301 agttttcttt acccttggga gtttagcacg tcttgcagga ttatcttgga taatatccaa
     3361 accaacagca taatcaaatg ctaagtttag catagactta ttacgctctt ttttattcct
     3421 agagcaatct aggttatcga gataattctg aacgtattta ggatcaatat tcactacttt
     3481 aacacctatt ccaaaagttt cccttatctc ttcgatatta cctctcagag aagctacaga
     3541 cgacctttta agttctttct tataaaacgc ccaccattca tctaaaagtt ccgtgaaaag
     3601 taactcagag gcctttaatt gcgctatttt ttctgttatt ttttgttcaa ggatttttcg
     3661 agctgcttta taagctcttg aattatcctt ctccatagta acggatacag gtttccatgt
     3721 tccagtgtaa ggatctttat aattttcacg aaatttgaat ttaccgttat cagtttcttc
     3781 tatccacatt tgttttttac ctcattttct gttaaaatag gtatagtaaa gagacctact
     3841 gcgaagcagg tttttactat actagattcg cctcacgctc tcctcgccaa atttgagcgt
     3901 ggggcttttt tgttagttga gaaaccaaga tgctatcgac tctaataatc cagcattttt
     3961 actttttgat actctatcta cttttaaaat agtcaatcgt aacttataag ttatatcttt
     4021 ctttttagta accacggtct ttaaatcgtt tttgagtgtt ttgtattgtc caccacgacc
     4081 agtaagggtt gcttccattt ggtaatgact agattttttt acataagggg ttaatcgttt
     4141 agctaagtct gctggaaagt agccaacaaa agttttgttt accatgactt ttatggcatt
     4201 tggatcgtga cgattaagag gttctctgac aacctctaca tcttttgttg agaccctgta
     4261 atacttgtag atatccttga agtttgattt tatgtagtca gaaataaaat ctttatcctt
     4321 atcccagtat ggtgatttct taccaatttc agccataact ttattagctt ctttttcatg
     4381 ataatttgtt cccattaaga gaaagttatc ttggaatatg acttttgcca taacagtctc
     4441 ctttaatatt ccctataaat atccacaact tcaccgattg tgcggatgtc gtcatccttt
     4501 gatagataga tttcctcata gcttttattt aaactttgca agtaccaagc gccatcataa
     4561 tcacgcttga gtttttttac aaagttttta ccattgactt ggaatatccc tatatcattc
     4621 atatcgactt ggctagatac cttgatgaat agcaaatcgt tgtcttgaat aagtggttcc
     4681 attgaatcgc cagctacttt tgcaatagta tcatactcat cggggacatc atcagaacgt
     4741 agtttaactt tcatgtgtaa attgtcttct tggtatgccc caagaccagc ggcaaccaat
     4801 ccctcaacgt agtcagtgat ataatcttct gtctcctcag attttttatc gaatatagaa
     4861 acggttttat tttgttcttc taattgttcg ttagcgaagt tgaggacttt ttcttgtcta
     4921 ggttgttcta gttggttgta tatagaaacg atttcaggtt gttcgttctg tattgtgatt
     4981 ttcccaagtt tactatctgt gagtcctaaa agatagtcag aggtaacgtt gaaaatctct
     5041 gctagttttt ttaggtcttt accttttgga aaattttcat tcttctccca ctttgaaaca
     5101 gtagtatagg ttttcatgcc caatatttct gaaagttcag tctgagtcat gttgcgccct
     5161 tctctcaact ttcttattcg gtcccctaat tgctccatag gatacctcct ttt
//
LOCUS       DAUELZ010000099         5131 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.100, whole genome shotgun sequence.
ACCESSION   DAUELZ010000099 DAUELZ010000000
VERSION     DAUELZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.100"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(141..449)
                     /locus_tag="VHI09_001442"
     CDS             complement(141..449)
                     /locus_tag="VHI09_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121310.1"
                     /translation="MTRLISQQILSEYVKSLEVEADKVVIPGQNTPVFSWDRDGNLSI
                     DTPLLKVRGESLATQADLTTISLTPGPKGELWQTSVERLDIKVDKHISLTFKNGVRID
                     "
     gene            complement(514..964)
                     /locus_tag="VHI09_001443"
                     /pseudo
     CDS             complement(514..964)
                     /locus_tag="VHI09_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1069..1289)
                     /locus_tag="VHI09_001444"
                     /pseudo
     CDS             complement(1069..1289)
                     /locus_tag="VHI09_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1308..1613)
                     /locus_tag="VHI09_001445"
     CDS             complement(1308..1613)
                     /locus_tag="VHI09_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ6121311.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQGM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(1613..1915)
                     /locus_tag="VHI09_001446"
     CDS             complement(1613..1915)
                     /locus_tag="VHI09_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEQ6121312.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLITSFEDAKTGNRQDVREFLFQLKENA"
     gene            complement(1970..2257)
                     /locus_tag="VHI09_001447"
     CDS             complement(1970..2257)
                     /locus_tag="VHI09_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121313.1"
                     /translation="MVPFGQGYKDMSPPSKELYKLMMVGKIQHVGHPVLKWMGQNVVM
                     RQAPAGNIKPDKEKSVEKIDGIVGLILGLDRCIRRQGDEASVYDERGILSF"
     gene            2162..3055
                     /locus_tag="VHI09_001448"
     CDS             2162..3055
                     /locus_tag="VHI09_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121314.1"
                     /translation="MTNMLDFPYHHEFIEFLGRWTHILITLPKRHHGQVDTAIPRYLL
                     VTIREKTGIVKLTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCVCPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEALNLIVLCPDCFLTYQADSRKKIVTALKNVKKILVSAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTLYQLVKNPVI
                     DNYLTIQKIIVNLDKQGKIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHMATL
                     QDIYFCQMIVSYFIQKCEVLE"
     gene            3055..3279
                     /locus_tag="VHI09_001449"
     CDS             3055..3279
                     /locus_tag="VHI09_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121315.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            3270..3998
                     /locus_tag="VHI09_001450"
     CDS             3270..3998
                     /locus_tag="VHI09_001450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEQ6121316.1"
                     /translation="MLIEMWSPVFKKHGEIREPICFHHGLNVIMGMDLADNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPIYFCFQFEKKFYFSRDTANPDIILVCDKSYSP
                     TGETMDLGIFLNRLKKRYHLDSPELSFRLAMSGFFRIAGKSNQNTDFPLQVYSSQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYIFISNLVGGKKQFEANVSE
                     IKRLEYDLAHLQDTQQDKIDSDDI"
     gene            4083..4928
                     /locus_tag="VHI09_001451"
     CDS             4083..4928
                     /locus_tag="VHI09_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEQ6121317.1"
                     /translation="MDISIEFGLYPTESDLTELQQYFPDTNLKKLYEVEAYHKKLETI
                     LDSEFSTERESLIAEIDELESQLTTLNQELQELGNIPNLSSEYLESYSKLTATINALK
                     EQNEAYLKESGLPKEKSGADADLKRSTEDILLDLEVKINSKMREFNNILYPDIRKAPQ
                     INLNAHNSYSFYTPDDDGTGTKFKGMIVFDLVMLYLTNLPALAHDSLLLSNVSYQATE
                     ALLKLYDQSRSLNKQVFLAFDKASSYSPDANQLLSENTVLRLSSNGNELYGISWNKGE
                     NSDEI"
     gene            4918..5127
                     /locus_tag="VHI09_001452"
     CDS             4918..5127
                     /locus_tag="VHI09_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ6121318.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIEDIVEIVDNNI"
BASE COUNT         1672 a         1081 c          886 g         1492 t
ORIGIN      
        1 catgaaatct aacctttacc ctttttaggg agtgcaaaat ccaaatgaat cgtgcatctc
       61 aactcccaat ataacttctt tcattatacc acagataaac taaaaaagga cgtgccgaat
      121 tgatacgccc ttgcctttta tcaatcaatc cgtacgccat ttttgaaggt taggctgatg
      181 tgcttgtcta cttttatatc caacctttca acacttgtct gccagagttc tccctttgga
      241 ccaggagtta aagagatggt tgtaaggtca gcttgtgtcg ctagactttc ccctctcacc
      301 tttaggagcg gtgtgtcaat ggaaagattc ccatccctat cccaactaaa gactggagta
      361 ttctgtcctg gaataacgac cttatcagct tcaacttcta ggctcttcac atactctgat
      421 agaatctgtt gggagataag ccttgtcatc catctgaact agagacggtc aactcatcaa
      481 tcttagcttt ttgaagtgct gcaacttgtg cttctaaggc atccgttgcc agataatcaa
      541 gagcagcttt cttatcagaa cgctcgcctt ctgctttagc agtagcgata ccataccact
      601 tttatctcgt ataatctgca cataggcatt tccccatatc aaaagatggc tcatgattgc
      661 ctctcgaaag ataaaggagg acatctctgg attgggttca tcatgaagaa gaaagtacag
      721 tgggtggtcc agctttttct ccttcccatt ttctgtccgt tcatagatgt gaataggaaa
      781 tgacgccacc gcttcagcaa gaatacgcac acaggcatag actgccgtcg tgtgcatagt
      841 cttaaactca tccacattct cgccactagt cgtgcgacca aagaggtagg aaaagtcctg
      901 accttcataa ctgttttgag gcttatctct agcccttttt tctaattaaa tcaagtagtc
      961 ccataagccc tccttatttt ggggtacaaa aaaagcacct cctttgaagt gctttcggta
     1021 tatttctttc aaagtaatca acagtctttc ttacaaaacc tagtattctc attctgtcaa
     1081 ttccttaaat gcgtcaagat gtcgtgataa tactgaagtc gcaatctcat ctaaccttgc
     1141 ttgttcaaca actgttgtca tctgctcatc accaaagttc catcagttga tttataaagg
     1201 ttaaactcct gattctcttg aatgccaaat aggtcaggta tcggaggata gatgtcatca
     1261 ccaatttgta tcgtctttac gagttccata catcactctc ccttacttta ccaattattc
     1321 atatgacgtg atccatagac gaccgcaaca attataactt catcttccag aacatgatat
     1381 acaatgcgat attttttgac aatcagttgt ctgaaggtat agccttttcc aatcagatcc
     1441 tcaatgatgg cacaacgttc aggaaaaatg gatagtgata acattccttg ggataacctc
     1501 tcaagaagat tatccgctgc ctgtggtgca cagagttcat cacgaacata gtgatagatg
     1561 ctcagcaaat ctgctttagc atcatccgac atggtgacct gatattcttt cattaagcat
     1621 tttccttcaa ttgaaataga aattctctga catcctgacg gttgccagtt ttcgcatctt
     1681 caaagctggt gataagctta tcataaaact tctcctgact catgtaatca acattaactc
     1741 gttgaggcgc ttcaggtaga gaaacatcaa atgggatgcc cccagtcaaa ataatctgat
     1801 tcaaaaacat atcaatcgca gttgacatag gaatacctaa gcgtttcaaa atatcatctg
     1861 ccgcactttt tactgaatca tcaactcgta aattcaaagt tcccgtttta gccatggtta
     1921 ttctccttat ttgtaacgac attgtatcac atttttggag ttgattcaac taaaaactca
     1981 atatccctcg ctcatcatag acgctagctt catcaccttg gcggcgaatg caacggtcaa
     2041 gccccaggat aagacctaca ataccgtcaa tcttctcaac agatttttcc ttatctggct
     2101 tgatgttgcc tgcaggggct tgtcgcatca ccacgttttg tcccatccat ttaaggactg
     2161 gatgaccaac atgttggatt ttccctacca tcatgagttt atagagttcc ttggacggtg
     2221 gactcatatc cttataaccc tgcccaaaag gcaccatggt caagtcgaca ccgcaattcc
     2281 aaggtatctt ctggtaacca tcagagagaa gacagggatt gtaaaattaa ccactgtgca
     2341 aaatacagcg ttcactaccc agtcgaatca actcaaaaaa caatacgggc agttcttgtt
     2401 aacagaatct aataactgtt gtgtatgccc aggctgtgat agaccactca ttctaaccag
     2461 tggcaactta gcagcagaaa attacgaggt ttcagccatt gaaaaagata aggacgctga
     2521 agcactgaat ctgatagtcc tctgcccaga ttgtttttta acctatcaag cagatagtcg
     2581 caaaaaaatt gtaacagctc taaaaaatgt taaaaagatt ctagtctctg cacataacag
     2641 ccaacaagcg atttctgata tgaagttaga cagtggaatt gtttcagttt tgactagtct
     2701 caataaacta aaatgtgatg aatatgatat ctcctatgac tcgaagcaat tgactgataa
     2761 gatatcacct gagaacaatc gtacgcttta tcaattggtt aaaaacccag ttattgataa
     2821 ctacttaacc attcaaaaaa tcatcgtaaa tttggacaaa caaggaaaaa ttgattacga
     2881 ggaaatccaa tatcaaacgc gatcgatgta taaaaagtta aaagcagcca agcataataa
     2941 tctggctatt ttcaatacta tttcggaaaa actccatatg gctacactac aggacatcta
     3001 cttctgccag atgattgtct cttactttat ccaaaaatgc gaggtacttg aataatgcaa
     3061 ttaccaaata aactttactc ctatcaaaaa agcactctgg cctatttacc tagagtgcta
     3121 aatgaaatca aaagtggaaa ctcaaacgtc aaagatatct ttcacgctat ctcagaagag
     3181 ttggatgatc ctacagattt cctatccata atggattgct tgtatgcatt gaacgctatt
     3241 gaaatgtcta atgaaggaga agtaaaatta tgcttataga aatgtggtct ccagtattta
     3301 agaaacacgg agaaattcgg gaaccaatct gctttcacca tgggctaaac gtgattatgg
     3361 ggatggattt agctgataac tctatcggca aatcctcctc actattagcc attgatttca
     3421 tctttggagg taatagctat ctaaaatcaa ttgcggttaa aaaattaggt gatcatccta
     3481 tctacttctg ctttcaattt gagaaaaagt tttatttttc aagagatacg gccaacccag
     3541 atatcattct agtgtgtgac aagagctact ctccaaccgg tgaaacaatg gacctaggaa
     3601 tttttcttaa cagacttaag aaacgatatc atttagattc tccagagcta tcattccgat
     3661 tagctatgag tggcttcttt aggattgcag gaaaaagtaa tcaaaatacc gatttcccct
     3721 tacaagttta ttcgagccaa aaatcttctg aatctattac tactctcatc caactgttta
     3781 atttgtatga taacattgca cgatacaaag aacgtctgaa agataagagc gaccagttaa
     3841 caacttttcg taatgctcga aagtatatct tcatctccaa cctggttggc ggtaaaaagc
     3901 aatttgaagc aaatgtctca gaaataaaaa gattggaata tgatttagca catctccaag
     3961 atactcagca agataaaatt gactctgatg atatttaaaa aaatcaacaa aaactccaac
     4021 ttagaaatac taagctggag ttagaaagca gtcttcggga caagcaaaga cgtcttaagc
     4081 tattggacat cagtatcgaa tttggcttat atcctactga atctgactta acagagcttc
     4141 aacaatactt cccagatact aatctcaaaa aattatatga agttgaagct taccacaaaa
     4201 aattggaaac tattttagat tccgaattct caactgaacg tgaatcccta atagctgaga
     4261 tcgacgaatt agagagtcaa ttaacaactc taaaccaaga acttcaagaa ctaggaaaca
     4321 tcccaaatct ttcaagtgag tacttagaaa gctactctaa actaacagct acaatcaatg
     4381 cactaaaaga acaaaacgag gcctacttaa aggagtctgg tctacccaaa gaaaaatcag
     4441 gagcggacgc tgacttgaaa cgcagtacag aagatatcct tctagatcta gaagtaaaaa
     4501 tcaacagtaa gatgcgtgaa ttcaataaca ttctatatcc agatattcgc aaagctcctc
     4561 aaattaacct caatgctcac aatagctact ccttctatac accagatgat gatggtacag
     4621 gaactaagtt caaagggatg attgtattcg acttagtaat gctctacctt actaatctgc
     4681 cagcactcgc acacgattca ttactcttga gtaatgttag ctaccaagcg actgaagcac
     4741 ttttgaagct gtatgaccag tcaaggtcat tgaacaaaca agtcttttta gccttcgata
     4801 aagccagctc ttattctcca gatgctaatc aactcttgtc agaaaatacg gtattgagac
     4861 tctctagtaa cggaaatgaa ctctacggta tttcatggaa caaaggagaa aactcagatg
     4921 agatttagtt acaataaatt atggaaactt cttattgata aaggttggac caaaacaaag
     4981 ctcaggcaag aagctgggat tagttcttcc acgatcgcaa aattgggaaa aggggaaaat
     5041 atcactactg atattttatt ggaaatttgt attgcacttg attgtaagat tgaagatata
     5101 gtagaaattg tagataataa tatctagaaa t
//
LOCUS       DAUELZ010000100         4982 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.101, whole genome shotgun sequence.
ACCESSION   DAUELZ010000100 DAUELZ010000000
VERSION     DAUELZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.101"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..2233)
                     /locus_tag="VHI09_001453"
     CDS             complement(<1..2233)
                     /locus_tag="VHI09_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEQ6121319.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLTSMP
                     DAIYLFTLIKFGLIGLAACYSFHRLYPKISAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVVLGLNKLITENKTRTYYLSISLLFIQNYYFGYMIALFCILYALVCLLSLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPVKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLTEVTQIKAGFAFIFLIIL
                     TSLPYSFSQQYNFLPLTLFLLSVFLLLGYTISLFSFRNSQIPSTFISAFILIFSLLES
                     GLNTYYQLQGINKEWGFPSRQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDS
                     MKFNYYGISQFSSVRNRLSSSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSE
                     GPLNKFGFTKLKTSGNTTLYQNHYSSPLAILTRNVYKDVNLNVNTLDNQTKLLNQLSG
                     KSLTYFNLQPAQLISGANQFNGQISAQASDYQNSVTLNYQINIPKHSQLYVSIPNIIF
                     SNPDAKEVRIQTDNHNFIYTTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHF
                     YSLS"
     gene            complement(2316..3935)
                     /locus_tag="VHI09_001454"
     CDS             complement(2316..3935)
                     /locus_tag="VHI09_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ6121320.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(4014..4886)
                     /locus_tag="VHI09_001455"
     CDS             complement(4014..4886)
                     /locus_tag="VHI09_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ6121321.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
BASE COUNT         1751 a          822 c          871 g         1538 t
ORIGIN      
        1 tagacaaact ataaaaatga ggttccttaa aactaatttg tttatttttt ggaaaaacaa
       61 acgaaaatgt agcaactttg gcatcggcga aatatcctaa atcaaaaaaa gagtaagcgt
      121 tatctgtagt atatatgaaa ttatgattat ctgtctgaat acgcacctct ttagcatcag
      181 gatttgaaaa tataatattg ggtatgctaa catagagttg actatgttta gggatgttaa
      241 tttgataatt aagggtaacg gagttttgat aatcagaagc ttgtgcagat atttgtccgt
      301 taaattgatt agcaccagaa ataagttgag ctggctgtaa gttaaaatag gttaaagatt
      361 tcccacttag ttggttaagt aatttggttt ggttatcaag ggtattgaca tttaggttga
      421 catctttgta aacattacgt gttaatatag ctaaagggct actatagtga ttttgataaa
      481 gagtagtatt cccgctagtt tttagttttg taaatccaaa tttatttaga ggtccttcgc
      541 taagattata ttttatacca agtagactgt ccataataat agtattgttt tggtatctaa
      601 ggtttaaatt cgtgccttta gactgaaatc ccaatcgatc caataaagaa ctagatagtc
      661 tatttcttac agaggaaaat tgtgaaatgc cgtaataatt aaatttcatg ctatcgttcc
      721 ctgtttgggg aagtagtctt tccattctaa aaaaaggttg actatttttt gacacagagt
      781 tgacaaggtt gttaatatcc tttaattgac tattatatat ctgtcgtgat gggaatcccc
      841 actccttatt aattccttga agttggtagt aggtgtttaa ccctgattca agaaggctaa
      901 agataagtat gaaagcagaa ataaaagtag atgggatttg agaatttcta aacgaaaata
      961 gtgaaatagt ataacctaat aataaaaaaa cactaagtaa aaaaagagtt aaaggtagaa
     1021 aattatattg ttgagaaaag ctataaggaa gagatgtcag tataatgagg aaaataaaag
     1081 caaaacctgc ttttatttga gtcacttctg ttagtcgaga gagagtctca catgcgagta
     1141 ataggataac tatggaaaaa gaccaagcgt agcgatgcaa aaacatattt ggtgagtgca
     1201 ttccctgcca aaaaagatca agtggctgta ggtaaaaact tattataata aaagttaaca
     1261 agcaggcatt ggctaatttt atttttaaag ggatactttc taaagtaaaa taaataacac
     1321 taagcattag gggaaataat cctacgtaaa tcataggcag agcattaaac ttggtagtat
     1381 cgtacactcc tattgagagc ttagcaggta tatccaaaaa ccaagcattg ttcgtaacta
     1441 actgttttac cggggataga ttctctccat aagttgacaa atctagatag gtaggaagta
     1501 ttactagagc acttgttaaa gcagcacata ttgacacagc tgtaaatcta acaaaagcga
     1561 taaacatttt gttaaaatca ttgagactta aaagacaaac taaggcgtaa agaatacaaa
     1621 aaagagcaat catgtagcca aagtagtaat tttgaatgaa taataatgat atcgaaagat
     1681 aataagttct ggttttattt tctgtgataa gtttatttaa tccaagtaca acaagtggaa
     1741 gaagaatgaa aacatctaac caagaattta gttccatttg acttgtcaag aagctcatta
     1801 agctataaaa aactgagatg gaaatcatca agaaagcact gatttttgga tataatctat
     1861 gaaaagaata gcatgcagct aatcctatta acccaaattt tatcaaggta aacaaataga
     1921 tagcatctgg catagaggtt aaattaaaaa agaaaagtaa tggagaaaag aaactgccaa
     1981 gataataaca cattaaagca taaaaattta ttcctagtcc gcttgtaaag gtataaaaaa
     2041 aactatcaga accgtgcatg atgttacgaa agttctgcgc aaaaataaca tactgatgaa
     2101 aagcatcact agctagaatt gttttatcac tattataata aatgcccatc gatagaagga
     2161 tgataaatat aatactaaga gggaacaaaa aactagcaag tccagcaatt atccatttat
     2221 tattattttt cataaattat atggtctttc gtttacaaat tttaaaaaag aggctaggaa
     2281 gagatcttct tagccacctc atttatcata aggttttatt tccaaagttc agcaactcgg
     2341 gcttggactt ctggattgtc aaggaattcg tcataagttt catctatacg gtcgataaca
     2401 ccattttttg aaataacaac gatgtggtta gcaatcgttt gaataaattc atggtcatga
     2461 ctggcaaaga taacagattc tttgaagtct ttgatcccat cattaagact tgaaatagat
     2521 tccaaatcca agtgatttgt tggatcatca agaataagaa cgtttgattt caatagcatc
     2581 aacttagaaa gcatgacacg cactttttcg ccaccggaaa gaacgttaac agatttttta
     2641 acttcgtctc cagagaaaag catccgtcct aggaaaccac gtaaaaaagt atcatcatct
     2701 tcaccttttg aggcaaattg acgtaaccat tcaagaatag attcttccgt ggcaaaatct
     2761 tttgaattgt ctttaggcaa gtaagaacga cttgttgtta ctccccattt aatggttcct
     2821 tcgtagtcaa tatcatctgc taaagcacgc ataagagcag ttgtttgaat atcattttga
     2881 ccaataatag cagctttgtc acctggacgt aaaatgaaac tgatattatc aatgattttc
     2941 tctccatcga tcgttacaga aagattttca acagtgagga agtcattccc catttcacgc
     3001 tctgctttaa aattaataaa tgggtattta cggcttgagg gaacaatttc ttctaattca
     3061 atcttatcaa gcattttttt acgagaggtt gcttgttttg atttagatgc gtttgctgag
     3121 aaacgggcaa cgaattcttg taattctttg attttttctt cggctttagc attgcggtca
     3181 gcttgtaaac gagcggccag ctcagaagat tgtttccaga aatcgtagtt acccacaaag
     3241 agtttgattt taccaaagtc aaggtcggcc atgtgagtac atactttatt caagaagtga
     3301 cggtcatggg aaacaacgat gactgtattt tcaaaatcaa tcaagaaatc ttccaaccat
     3361 gaaatagatt gaatatcaag accattggtt ggctcgtcaa gtaataaaac gtcaggctta
     3421 ccaaagagcg ctttagcaag aaggactttc actttatctc cattagccag ttcactcata
     3481 ttttggtagt gaaggtcttc tggaatattt agattttgga gaagttgaga agcttcactt
     3541 tctgcctccc agccacctaa ttcagcaaag ataccttcta gctcagcggc acgaacccca
     3601 tcttcttctg aaaaatctgc tttcatatag atagcgtctt tttctttcat gatattgtaa
     3661 agttgctcat tacccatgat tacaacatca atcgcacgtt cttcttcata atcaaaatga
     3721 ttttggcgaa gaacagataa gcgttcgtca ggtcctaaag agatatgacc agtacttggt
     3781 tcaatatctc ctgcaaggat tttaagaaaa gttgattttc cggcaccatt cgcaccgatt
     3841 aagccgtaag tattgccggc tgtgaatttg atattaacat catcaaatag ttttcgatca
     3901 ctaaagcgta gtgatacatc agaaactgta agcaatttgt cacctcattt tatttatcta
     3961 agcttcattg tacctaaaat agtccccttt ttcaatcaga aaagggctaa tcactacata
     4021 ggcaataaaa cgtctttgtc ttcacgtttg tattgtttag tcaaactaaa tcctcttccc
     4081 ataacttgag ctgctggcat gacaacaata aaggctgtat cgtcaatttc gaggattttt
     4141 tcttggagag taggtaattc gtgacttgat acaatagcca ttaacataat tttatcagaa
     4201 gtagtataac caccgcgaat aggaagttta gtcactccac gatccataac agttgtgata
     4261 tattctcgaa tagcttgata atctttggaa ataatcataa catttttgga tgagtcaaag
     4321 ccattttcca taacactaat cacataacca attacgaaaa gtccaatcgt agaatacatc
     4381 acgtcatctg cagaaagtgc aataaatccc ataaggacac taataccatc tacaattgtc
     4441 attgcaactc caagagataa aggagagtat ttatgaagta tttgtgttag aattccagtt
     4501 cctcctgtcg aagagtttcc ccaaaaaacc attcctaaac caatgccaca gataatacca
     4561 ccaaaaatag ctgctaacaa ctgattgtgt gtcagagtag gtaatgaatt ggttgaacgg
     4621 atggcaatag gatatatcca agaaccataa agtgttttaa taaaattttg ttttccaaga
     4681 aagaagtaac acattaataa taagggaata ttactagcca ttaaaaaaag cgacggacta
     4741 atgccaaata gagcttttat aacaactgca ataccaacca taccccctga tgcaatatga
     4801 ttgtcgacaa acattgtatt gaaaccaata gctgttatga atgagccaat tgtgacaagt
     4861 agtaaatcaa tccctcgttt tttcatgctt ttaatctcct ttataactgg ttaaaaatag
     4921 tagactcatt atctcttaaa tgtactgaga ccgtcaagtc atattttcaa aaaatctggt
     4981 ga
//
LOCUS       DAUELZ010000101         4959 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.102, whole genome shotgun sequence.
ACCESSION   DAUELZ010000101 DAUELZ010000000
VERSION     DAUELZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.102"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            18..860
                     /locus_tag="VHI09_001456"
     CDS             18..860
                     /locus_tag="VHI09_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEQ6121322.1"
                     /translation="MLSLLALSTFFLSACSSWIDKGESITAVGSTALQPLVEAVADEF
                     GSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVDHQVAVAGL
                     AVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGSRATFDSVI
                     MKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFKANAKNVAT
                     NDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDMEVVKSHDGK
                     VTKR"
     gene            871..1806
                     /gene="pstC"
                     /locus_tag="VHI09_001457"
     CDS             871..1806
                     /gene="pstC"
                     /locus_tag="VHI09_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEQ6121323.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTILSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATRWQTIWRVVLNAARP
                     GIFTAIIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            1796..2683
                     /gene="pstA"
                     /locus_tag="VHI09_001458"
     CDS             1796..2683
                     /gene="pstA"
                     /locus_tag="VHI09_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEQ6121324.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSKMTAAK"
     gene            2699..3502
                     /gene="pstB"
                     /locus_tag="VHI09_001459"
     CDS             2699..3502
                     /gene="pstB"
                     /locus_tag="VHI09_001459"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121325.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            3515..4273
                     /gene="pstB"
                     /locus_tag="VHI09_001460"
     CDS             3515..4273
                     /gene="pstB"
                     /locus_tag="VHI09_001460"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121326.1"
                     /translation="MTEPILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            4341..>4959
                     /gene="phoU"
                     /locus_tag="VHI09_001461"
     CDS             4341..>4959
                     /gene="phoU"
                     /locus_tag="VHI09_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEQ6121327.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWI"
BASE COUNT         1452 a          922 c         1039 g         1546 t
ORIGIN      
        1 tgaaaaaaaa attctttttg ttaagtcttt tagccctatc aactttcttt ttatccgcat
       61 gttctagctg gattgataaa ggcgagtcaa taaccgctgt aggatcaaca gcactacaac
      121 ccttagtaga agcagtagct gatgaatttg gaagcagtaa tctaggcaag actgtcaatg
      181 ttcaaggtgg tggttcaggt acagggttgt ctcaagttca atcaggagct gtccaaattg
      241 gaaatagtga tgtctttgcg gaagaaaaag atggtattga tgcttctaaa ttagttgatc
      301 atcaagtagc tgttgcagga cttgcagtta ttgccaatcc taaagtcaag gtttccaatc
      361 tcagtagtca gcagttgcaa aagatttttt caggagaata taccaattgg aaacaagttg
      421 gaggagaaga tcttgcgatt tcagtgatca accgagcagc aagttctggc tcacgagcaa
      481 cctttgacag tgttatcatg aaaggggtca acgctaaaca aagtcaagag caagactcca
      541 atgggatggt taaatcgatt gtttcacaaa caccaggtgc catttcttac ctttcctttg
      601 cctacgttga ttcatctgtt aaatctttgc aattaaatgg gtttaaggca aatgctaaga
      661 acgtggctac aaatgattgg ccaatctggt cctacgaaca catgtatacc aaagataaac
      721 caacagggtt gaccaaggaa tttcttgatt atatgttttc agatgaagta caacagaaca
      781 ttgttacaca tatgggatat atttcgataa atgatatgga agtggtcaaa tctcatgatg
      841 gaaaagtaac aaaaaggtaa tggaggaatt atggaaaatc aggaattggc taaaaaatta
      901 gcctcaccat caaagaattc aagacttgaa acctttggcc gcaccattac gtttttgtgt
      961 ctggctttga ttgtttttat cgttgcgatg attctgatct tcgttgctca aaaagggttg
     1021 tcaaccttct ttgttgacaa ggttaatctt tttgatttct tgtttggaaa ggagtggcaa
     1081 ccaagtgtga aaaatgcggc cggcattcct tatcttggtg ctctgccaat gattacagga
     1141 tcctttttgg ttaccatttt atctgctatt attgccaccc catttgccat tggcgcagcc
     1201 gtttttatga ctgaaatctc acctaaatat ggcgctaaat tattacagcc tgcggttgag
     1261 cttttggttg gaattccttc ggttgtttat gggtttatcg gtttgcaagt gattgttcct
     1321 tttatgcgct ctatctttgg tggcacaggt tttggaatcc tatctggggt ctgtgtcttg
     1381 tttgttatga ttttaccaac agtgactttt atgacaacag acagtctgcg ggcggtgcct
     1441 cgtcattacc gcgaagcgtc tatggctatg ggagcaaccc gttggcaaac catttggcgt
     1501 gttgttctta atgcagctcg tccaggtatt tttactgcca ttattttcgg gatggcgaga
     1561 gcttttggtg aagccttagc cattcaaatg gttgtcggta actctgctgt aatgccaagt
     1621 tcactaacga caccagccgc aaccttaacg tctgtcttga caatgggtat tggtaacacg
     1681 gttatgggaa cggtccaaaa taatgtgctt tggtcccttg ccctagtctt attattaatg
     1741 agtctggcct tcaattccct tgtcaaatta atcacgaaag aaagaaagag aaattatgaa
     1801 cgctaaaaaa gtcgataaag tagcaactgg tactctttat accattgctg gaattattgt
     1861 agctatttta gcttccttaa ttctatatat ccttgtccgt ggcttgccac acatcagctg
     1921 gtccttttta acaggaaaat cgtcttcgta cgaagctggt gggggaattg gtatccagtt
     1981 gtataattcc ttcttcctat tgattgttac cctgattatt tccattcctt tatcaactgg
     2041 agcggggatt tacttggctg aatatgccaa aaaagggcct gttaccaact ttattagaac
     2101 ctgtattgag attctgtctt ccctaccatc tgtggttgta gggctctttg gttacttgat
     2161 tttcgttgtg cagtttgaat atggcttttc tattatttca ggggctcttg ctttgacggt
     2221 ctttaatctt cctcaaatga cccgtaatgt tgaagatagt ttactgcatg ttcatcatac
     2281 ccaaagagaa gcaggattag ctttaggcct gtcacgctgg gaaacagttt tttatgtggt
     2341 tatcccagaa gctctcccgg ggatggtaac aggtattgtc ttagcttcag gtcgtatttt
     2401 tggtgaagcg gcagcgctta tctatactgc tggtcaatca gcaccagctc tggattggtc
     2461 aaattggaat ccacttagtg ttactagtcc aatctctatt ttccgtcaat ctgagaccct
     2521 tgctgttcat atttggaaag tcaatagcga agggacaatt ccagatgcta ctcttgtatc
     2581 cgcaggaagt gcagcagtgc tattaatttt tatccttatt ttcaactttt cagctcgctt
     2641 tattgggaag aaacttcatt ctaaaatgac agcagcgaaa taagatattg gagaaatcat
     2701 gacagaatat aattggaatg aacgtcatat catcactttt cctgaagaaa cgcttgctct
     2761 agctaccaag gatttacatg tttactatgg tgcgaaagaa gctatcaaag ggattgatat
     2821 gcagtttgaa aaacataaaa taacagcctt gattggtcca tctggttgtg gcaaatcaac
     2881 ttatcttcgt agtcttaacc gcatgaacga taccattgat attgctcggg ttacaggtga
     2941 gattctttat cagggcattg atgttaatcg aaaagacatg aatgtttacg agattcgcaa
     3001 gcacttagga atggtttttc aaagacccaa tcctttcgct aaatccattt ataaaaacat
     3061 tacctttgcc catgaaagag ctggtgtgaa agacaaaaag gttttagatg aaattgttga
     3121 gacttcactg aaacaagccg cgctttggga tcaagttaaa gatgaccttc acaaatcagc
     3181 cttcacctta tcagggggtc agcaacaacg cttgtgtatt gctagagcca tttctgtcaa
     3241 acctgatatt ctattaatgg atgaaccagc ctctgccctt gacccgattg caacgatgca
     3301 gctggaagaa accatgtttg aattgaagaa gaattacacg attattattg tgacccacaa
     3361 tatgcaacaa gcagctcgtg ccagtgatta cactgccttc ttctatttag gcaacctcat
     3421 tgaatatgat aagacacgta atattttcca aaatgcgcaa tgtcagtcaa caaacgacta
     3481 cgtttcaggg cactttggtt agaaaggtaa tatgatgaca gaacctattt tacagatcag
     3541 agacctttct gtttactaca atcaaaagaa aacgttaaaa gatgtctctt tggaccttta
     3601 tccaaatgaa ataacagctt taattggtcc ttctggatct gggaaatcaa cgctcttgcg
     3661 ttctatcaac cgtatgaatg atttgaatcc tgaagtaacc attacaggct ctatcgtcta
     3721 taacggacac aatatttata gtccaaggac agatacggtt gatctacgca aagaaattgg
     3781 tatggtattc caacaaccca acccatttcc gatgtctatt tatgagaacg tggtttacgg
     3841 ccttcgctta aaaggtatca gagataaatc aatcttagac catgctgttg aaagctctct
     3901 taaaggggct tccatttgga atgaagtcaa agatcgttta catgattctg cggttggctt
     3961 atcaggcgga cagcaacaga gagtttgcat agctagagtt cttgctacca gccccagaat
     4021 aatcctttta gatgaaccta cctcagctct tgatcctatc tcagctggga aaattgaaga
     4081 aacattattg ttattaaaaa aagattatac actggctatt gtgacccgct caatgcagca
     4141 agcctcacgt ttatcagatc gaacaggttt tttcttggag ggtgacttac ttgagtgtgg
     4201 tcctacaaaa gctatgttta tgaatcctaa gcgcaaagaa accgaagact atatttctgg
     4261 taaatttggt tgagaaagac gtatatggtt gacccctctg tgtttttatt ataatgacta
     4321 gattgtagga agaaaaaatc atgttaagaa cgaaatttga agaagaatta gataaattgc
     4381 ataaccagtt ttattcaatg ggaacggaag tattggcaca aatcaataaa actgttcgtg
     4441 cctttgttag tcatgatcgt gaattggcca aagaagttat cgaagaagat gacactatta
     4501 ataattttga gacaaagctt gaaaagaaat cgctggaaat tattgcccta caacagccag
     4561 tttcgaatga tcttagaatg gttattactg ttttaaaggc atcaagtgat attgaacgta
     4621 tgggtgacca tgctgcttcg attgccaagg ccactattcg tatgaaaggt gaggagcgta
     4681 ttccagtggt tgaagaacaa atcaacctta tgggaaaagc tgtgaaacag atggtagaag
     4741 aagcccttaa tgcatatatc aatgctgacg atacaaaagc ttatgaaatc gcagcttctg
     4801 atgagattat cgatcaatat ttccgtaata tccaaacctt agctgttgaa gaaatccgta
     4861 agtctccaga tgctgttttt gcaggaaaag aatattttca agtattaatg tatttggaac
     4921 gtattggtga ctatgctcgc aacatttgtg aatggattg
//
LOCUS       DAUELZ010000102         4916 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.103, whole genome shotgun sequence.
ACCESSION   DAUELZ010000102 DAUELZ010000000
VERSION     DAUELZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.103"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            3..515
                     /gene="lepB"
                     /locus_tag="VHI09_001462"
     CDS             3..515
                     /gene="lepB"
                     /locus_tag="VHI09_001462"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ6121328.1"
                     /translation="MVIGAILLRIFVFSTFKVSPETANTYLKSGDLVTIKKNIQPKYK
                     DFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYLEKMKAHYLNHAPFGT
                     LYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLINASQIKGLVTFRVLP
                     LSDFGFVEVE"
     gene            691..2505
                     /gene="glmS"
                     /locus_tag="VHI09_001463"
     CDS             691..2505
                     /gene="glmS"
                     /locus_tag="VHI09_001463"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEQ6121329.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLATTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2701..3036
                     /locus_tag="VHI09_001464"
     CDS             2701..3036
                     /locus_tag="VHI09_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEQ6121330.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVA
                     LDSNGVRLSDGDTITVIKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            3143..3784
                     /locus_tag="VHI09_001465"
     CDS             3143..3784
                     /locus_tag="VHI09_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ6121331.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKVPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            3794..4423
                     /locus_tag="VHI09_001466"
     CDS             3794..4423
                     /locus_tag="VHI09_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121332.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKADAKEKALDLLARLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVESLIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            4439..>4916
                     /locus_tag="VHI09_001467"
     CDS             4439..>4916
                     /locus_tag="VHI09_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ6121333.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQNKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGY"
BASE COUNT         1508 a          798 c         1048 g         1562 t
ORIGIN      
        1 ttgtggttat tggggctatt ttgttgagaa tctttgtttt ttcaactttt aaagtttctc
       61 cagaaacagc taatacttat ttaaagagtg gtgatttagt cacaatcaaa aaaaatattc
      121 agcccaaata taaagatttt gtggtttata gagttggaaa aaaagattat gtcagtcgag
      181 tcattgctgt tgaaggcgat agcgtgactt atatggatga tattttttac ctcaataata
      241 tggtagagtc acaggcttac cttgaaaaga tgaaagcaca ttacttgaat catgcaccgt
      301 ttggcacatt gtatacagat gattttacag ttgctaccat cacagctgat aagtatcaaa
      361 aagttcctaa ggggaagtat cttcttttga atgataaccg gaaaaatacg aatgacagtc
      421 gtcgatttgg gttaataaat gcctcgcaga ttaaaggttt agtgaccttt agagttttgc
      481 ctctcagcga ttttggattt gtagaagtag agtagttgtc aggctactct acttttttta
      541 taattagact ataccaattt tatgtttgac aagacattgt gggtattata tactgtaaac
      601 ataatgtttt tatcggtata atatgactat accaattatt gaaattatga gagaaagtag
      661 caagttaacg aacttgttaa ggtaattaag atgtgtggaa ttgttggagt tgttggaaat
      721 cgcaatgcaa cggatatttt aatgcaaggc cttgaaaagc ttgaataccg gggttatgat
      781 tcagcaggaa tttttgtggc taatgccaat caaacaaact tgattaaatc agtggggcgg
      841 attgctgatt tgcgtgccaa gattggcatt gatgttgctg gttcaacagg gattggtcac
      901 acccgttggg caacgcatgg ccaatcaaca gaggataatg cccatcctca cacgtcacaa
      961 actggacgtt ttgtacttgt tcataatggt gtgattgaaa attaccttca cattaaaaca
     1021 gagttcctag ctggacatga ttttaagggg cagacagata ctgagattgc agtacacttg
     1081 attggaaaat ttgtggaaga agacaagttg tcagtactgg aagcttttaa aaaagcttta
     1141 agcattattg aaggttccta tgcctttgcg ttaatggata gccaagcaac tgatactatt
     1201 tatgtggcta aaaacaaatc tccattgttg attggacttg gtgaaggtta caacatggtt
     1261 tgttcagatg ccatggccat gattcgtgaa accagtgaat ttatggaaat tcatgataag
     1321 gagctagtta ttttaaccaa agataaggta actgttacag actacgatgg taaagagctg
     1381 atacgagatt cctacactgc tgaattagac ttatctgata ttggcaaagg gacttatcct
     1441 ttctatatgc tgaaagaaat tgatgagcaa ccaaccgtaa tgcgtcaatt aatttcaact
     1501 tatgcagatg aaactggtaa cgtacaggtt gatccagcta tcattacctc tatccaagag
     1561 gctgaccgtc tttatatttt agcggcaggg acttcctacc atgctggttt tgcaacaaaa
     1621 aatatgcttg agcaattgac agatacacca gttgagttgg gagtggcttc tgagtggggt
     1681 taccacatgc ctctgcttag caagaaacca atgtttattc tactaagcca atcaggagaa
     1741 accgcagata gtcgtcaagt tttagtaaag gcaaatgcta tgggcattcc gagtttgaca
     1801 gtaactaacg ttccaggatc aaccttatca cgtgaagcaa catacaccat gttgattcat
     1861 gctggacctg aaattgctgt tgcgtctaca aaagcttaca ctgcacaaat tgctgccctt
     1921 gcctttttgg ctaaggcagt tggtgaggca aatggtaagc aagaagctct tgactttaac
     1981 ttggtacatg agttgtcatt ggttgcccaa tctattgagg cgactttgtc tgaaaaagat
     2041 ctcgtggcag aaaaggttca agctttgcta gctactactc gtaatgcttt ttacatcggg
     2101 cgtggcaatg attattacgt tgcgatggaa gctgctttga aattaaaaga gatttcttat
     2161 attcaatgcg aaggctttgc ggctggtgaa ttgaaacatg gaaccatttc attaattgag
     2221 gaggacacgc cagtaatcgc tttaatatcg tctagtcagt tggttgcctc tcatacgcgt
     2281 ggtaatattc aagaagttgc tgcccgtggg gctcatgttt taacagttgt ggaagaaggg
     2341 cttgaccgtg agggagatga cattattgtc aataaggttc atcctttcct agccccgatt
     2401 gctatggtca ttccaactca actgattgct tactacgctt cattacaacg tggacttgat
     2461 gttgataagc cacgtaattt ggctaaagct gtaacagtag aataaatgat tataatggat
     2521 aaggcaacca tctgttaggt ggttgctttt tgtttttagg agaatagcag agcttagaaa
     2581 atggccttga gaaaagctat acaagaagaa tagtcatctt ctgatgagaa cgtattcatc
     2641 ttgtcaaatc attgattcta aagtaaaatg gtaacattaa gaaatgttta ggagtgtaag
     2701 atgtcattac caaattgttt gcaatgccag tctgaatatg tttacgaaga tggtctttta
     2761 ttaatctgtc caatgtgtgc ttttgagtgg acaccaggtg aggaagcgac tgaagaagaa
     2821 ggacttgttg ctttagatag caatggtgtt cgattgtctg atggcgatac tattactgtt
     2881 attaaagatt taaaagttaa aggtgctcca aaagacctga aacaagggac tcgtgttaaa
     2941 aatattcgcc tggtggaagg tgatcataac attgattgta agattgatgg gtttggcgcg
     3001 atgaaattga aatcagaatt tgtcaagaaa atctaataat aatacgcaac cagctgatct
     3061 ggttgtattt ttataaaaaa atctctcccc aaatacgttc aaatttggta taatagtaaa
     3121 atattcagtt ttaggaggca acatgaccta tattcagcaa gttttaccca gcttattaga
     3181 tggtgccttg gtgaccttac aagtattctt tattgttatc attctttcta tacccttagg
     3241 ggctatttta gctttcttga tgaaggttcc ctttaaaccg ctacagtggt ttttgaccct
     3301 atacgtgtgg atgatgcgag ggacaccatt actacttcaa ttgatttttt tctattatgt
     3361 tttgccaagt gtggggatta gttttgatcg aatgccagct gctattttgg cgtttacttt
     3421 gaattatgct gcctactttg ctgaaatttt tagaggtggg attgaggcta ttccaaaagg
     3481 tcaatatgaa gcagctaaag tattaaagtt aaaacctctt caaaccattc gttatattat
     3541 tttgcctcaa gtgtttaaaa ttgtgttacc aagtgttttc aatgaagtca ttaatttggt
     3601 caaagattct tcccttgtct atgtactcgg tgtaggagat cttttattag caagtaagac
     3661 ggcagccaat agggatgcaa ccttagcccc tatgtttatc gctggtctta tctatttgct
     3721 tttaattgga ctggtcacga ttatttcaaa acaagttgaa aaacggttta attattatca
     3781 gtaaggaggc ttcatgttag aactcaaaaa tatttccaaa cagtttggtc aaaaaaccat
     3841 ttttgatggc tttaatctaa ccgttcaaga cggagaagtg ctctctctgg taggcccatc
     3901 aggaggcggt aagacaacac tcttacggat gttagcaggt cttgaatcta ttgattcagg
     3961 tcaagtcttt tacaatggag aagatgttgg tattgatcat ttggaaaatc gtaacttgct
     4021 tggatttgtt tttcaagatt ttcaattgtt tcctcatctt accgttttag acaacctaac
     4081 gctgtctcca acaattacaa tgggcaagca aaaggctgac gctaaagaaa aggctttgga
     4141 tttactggca cgtttaggat taaaagaaca tgcccaagtt tatccctatt cgttatctgg
     4201 aggtcaaaaa caacgggttg ctttggcaag agccatgatg attgaccctc aaattatcgg
     4261 ttacgatgaa ccgaccagtg ctctagatcc tgaattacgt cagacggtag aatccttgat
     4321 tgttcaaaac cgtgaaatgg gaataactca aattgtagtc actcacgatc ttgtttttgc
     4381 cgaagccatc tcagatcgta ttattagggt taatcctaaa tagaagagga cccaaaagat
     4441 gattataaaa aaaagaaccg tagcaatttt agccatagct agtagctttt tcttggtagc
     4501 ttgtcaagct actaaaagtc ttaaatcagg agatgcttgg ggagtttacc aaaagcaaaa
     4561 aagtattaca gttggttttg acaatacgtt tgttcctatg ggctataagg atgaaagcgg
     4621 caaatgcaaa ggttttgata ttgatttggc taaagaagtt tttcaccaat atggactcaa
     4681 ggttaatttt caagctatta attgggacat gaaagaagca gaactaaaca atggtaaaat
     4741 tgatgtaatc tggaatggtt attcaataac taaggagcgt cagaataagg ttgcctttac
     4801 tgattcttac atgagaaatg aacaaattat tgttgtcaaa aaaagatctg atattaaaac
     4861 aatatcagat atgaaacata aagtgttagg agcacaatca gcttcatcag gttatg
//
LOCUS       DAUELZ010000103         4914 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.104, whole genome shotgun sequence.
ACCESSION   DAUELZ010000103 DAUELZ010000000
VERSION     DAUELZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.104"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            122..1670
                     /locus_tag="VHI09_001468"
     rRNA            122..1670
                     /locus_tag="VHI09_001468"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1722..1794
                     /locus_tag="VHI09_001469"
     tRNA            1722..1794
                     /locus_tag="VHI09_001469"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1755..1757,aa:Ala,seq:tgc)
     gene            2093..>4914
                     /locus_tag="VHI09_001470"
     rRNA            2093..>4914
                     /locus_tag="VHI09_001470"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1389 a         1019 c         1422 g         1084 t
ORIGIN      
        1 aagacctttg agaactgaat aagacgaacc aaacgtgagg gtgatatgga gacatattac
       61 ccgtcaagaa acgagaaata aatctgtcag cgacagaagg aacgagtaag ttcaaacgca
      121 aattaaagag agtttgatcc tggctcagga cgaacgctgg cggcgtgcct aatacatgca
      181 agtagaacgc tgagaactgg tgcttgcacc ggttcaagga gttgcgaacg ggtgagtaac
      241 gcgtaggtaa cctacctcat agcgggggat aactattgga aacgatagct aataccgcat
      301 aagagagact aacgcatgtt agtaatttaa aaggggcaat tgctccacta tgagatggac
      361 ctgcgttgta ttagctagtt ggtgaggtaa aggctcacca aggcgacgat acatagccga
      421 cctgagaggg tgatcggcca cactgggact gagacacggc ccagactcct acgggaggca
      481 gcagtaggga atcttcggca atgggggcaa ccctgaccga gcaacgccgc gtgagtgaag
      541 aaggttttcg gatcgtaaag ctctgttgtt agagaagaat gatggtggga gtggaaaatc
      601 caccaagtga cggtaactaa ccagaaaggg acggctaact acgtgccagc agccgcggta
      661 atacgtaggt cccgagcgtt gtccggattt attgggcgta aagcgagcgc aggcggtttt
      721 ttaagtctga agttaaaggc attggctcaa ccaatgtacg ctttggaaac tggagaactt
      781 gagtgcagaa ggggagagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag
      841 gaacaccggt ggcgaaagcg gctctctggt ctgtaactga cgctgaggct cgaaagcgtg
      901 gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag tgctaggtgt
      961 taggcccttt ccggggctta gtgccggagc taacgcatta agcactccgc ctggggagta
     1021 cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcgg tggagcatgt
     1081 ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatcccga tgcccgctct
     1141 agagatagag ttttacttcg gtacatcggt gacaggtggt gcatggttgt cgtcagctcg
     1201 tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccctattgtt agttgccatc
     1261 attaagttgg gcactctagc gagactgccg gtaataaacc ggaggaaggt ggggatgacg
     1321 tcaaatcatc atgcccctta tgacctgggc tacacacgtg ctacaatggt tggtacaacg
     1381 agtcgcaagc cggtgacggc aagctaatct cttaaagcca atctcagttc ggattgtagg
     1441 ctgcaactcg cctacatgaa gtcggaatcg ctagtaatcg cggatcagca cgccgcggtg
     1501 aatacgttcc cgggccttgt acacaccgcc cgtcacacca cgagagtttg taacacccga
     1561 agtcggtgag gtaacctatt aggagccagc cgcctaaggt gggatagatg attggggtga
     1621 agtcgtaaca aggtagccgt atcggaaggt gcggctggat cacctccttt ctaaggaaat
     1681 ggaacacgtt tatcgtctta tttagttttg agaggtcttg tggggcctta gctcagctgg
     1741 gagagcgcct gctttgcacg caggaggtca gcggttcgat cccgctaggc tccatcagga
     1801 tacaatccta ctaaacttaa tacaagtgaa gttgaacacg caactcactt cctaggaaaa
     1861 tagacaatct tcgcatgtgt gcaaggcaca catggtcaga ttcctaattt tctacagaag
     1921 tttcgctaaa gcgagcgttg cttagtatcc tatataatag tccattgaaa attgaatatc
     1981 tatatcaaat tccacgatct agaaatagat tgtagaaaag taacaagaaa ataaaccgaa
     2041 aacgctgtga ctatttaata agttttctag tttaaaaaaa ctaggttaat aaggttaagt
     2101 taataagggc gcacggtgga tgccttggca ctagaagccg aagaaggacg tgacaaacga
     2161 cgaaatgctt tggggagctg taagtaagcg ctgatccaga gatgtccgaa tgggggaacc
     2221 cactaactaa tggttagtat ccataactgt taaggttatg agaaggaaga cgcagtgaac
     2281 tgaaacatct aagtagctgc aggaagagaa agcaaacgcg attgccttag tagcggcgag
     2341 cgaaacggca ggagggcaaa ccggagtgtt tacacttcgg ggttgtagga ctgcgacgtg
     2401 ggactttaaa aggatagaag aattacctgg gaaggtaagc caaagagagt aaaagcctcg
     2461 tatttaaaat tcttttgagc cctagcagta tcctgagtac ggcgagacac gtgaaatctc
     2521 gttggaatct gggaggacca tctcccaacc ctaaatactc tctagtgacc gatagtgaac
     2581 cagtaccgtg agggaaaggt gaaaagcacc ccgggagggg agtgaaatag aacctgaaac
     2641 cgtgtgccta caacaagttc gagcccgtta atgggtgaga gcgtgccttt tgtagaatga
     2701 accggcgagt tacgatatga tgcgaggtta agttgaagag acggagccgt agggaaaccg
     2761 agtcttaata gggcgattta gtatcatgtt gtagacccga aaccatgtga cctacccatg
     2821 agcaggttga aggtgaggta aaactcactg gaggaccgaa ccagggcacg ttgaaaagtg
     2881 cttggatgac ttgtgggtag cggagaaatt ccaaacgaac ttggagatag ctggttctct
     2941 ccgaaatagc tttagggcta gcgtcgatgt taagtctctt ggaggtagag cactgtttgg
     3001 gtgaggggtc catctcggat taccaatctc agataaactc cgaatgccaa cgagatatca
     3061 tcggcagtca gactgcgagt gctaagatcc gtagtcgaaa gggaaacagc ccagaccacc
     3121 agctaaggtc ccaaaataac tgttaagtgg aaaaggatgt ggggttgcac agacaactag
     3181 gatgttagct tagaagcagc tattcattca aagagtgcgt aatagctcac tagtcgagtg
     3241 accctgcgcc gaaaatgtac cggggctaaa acagtttacc gaagctgtgg atgacacaaa
     3301 agtgtcatgg taggagagcg ttctatgtgt gaagaaggtg taccgtgagg agcgctggaa
     3361 cgcatagaag tgagaatgcc ggtatgagta gcgcaagaca ggtgagaatc ctgtccaccg
     3421 taagactaag gtttccaggg gaaggctcgt ccgccctggg ttagtcggga cctaaggaga
     3481 gaccgaaagg tgtatccgat ggccaacagg ttgatattcc tgtactagag tatatagtga
     3541 tggagggacg cagtaggcta actaaaccgg acgattggaa gagtccggct aagcagtgag
     3601 gtgtaagatg agtcaaatgc ttatctttat aacattgagc tgtgatgggg agcgaatttt
     3661 agtagcgaag ttagtgatgt cacactgcca agaaaagctt ctagcgttta atgatactct
     3721 acccgtaccg caaaccgaca caggtagtcg aggcgagtag cctcaggtga tcgagagaac
     3781 tctcgttaag gaactcggca aaatgacccc gtaacttcgg gagaaggggt gctgacttag
     3841 gtcagccgca gtgaataggc ccaagcaact gtttatcaaa aacacagctc tctgctaaat
     3901 cgtaagatga tgtatagggg gtgacgcctg cccggtgctg gaaggttaag aggagggttt
     3961 agcgcaagcg aagatctgaa ttgaagcccc agtaaacggc ggccgtaact ataacggtcc
     4021 taaggtagcg aaattccttg tcgggtaagt tccgacccgc acgaaaggcg taatgatttg
     4081 ggcactgtct caacgagaga ctcggtgaaa ttttagtacc tgtgaagatg caggttaccc
     4141 gcgacaggac ggaaagaccc catggagctt tactgcagtt tgatattgag tatctgtacc
     4201 acatgtacag gataggtagg agccattgac ttcgggacgc cagtttcgaa tgaggcgttg
     4261 ttgggatact acccttgtgt tatggctact ctaacccaga taggttatcc ctatcggaga
     4321 cagtgtctga cgggcagttt gactggggcg gtcgcctcct aaagagtaac ggaggcgccc
     4381 aaaggttccc tcagattggt tggaaatcaa tcgcagagtg taaaggtata agggagcttg
     4441 actgcgagag ctacaactcg agcagggacg aaagtcgggc ttagtgatcc ggtggtaccg
     4501 aatggaaggg ccatcgctca acggataaaa gctaccctgg ggataacagg cttatctccc
     4561 ccaagagttc acatcgacgg ggaggtttgg cacctcgatg tcggctcgtc gcatcctggg
     4621 gctgtagtcg gtcccaaggg ttgggctgtt cgcccattaa agcggcacgc gagctgggtt
     4681 cagaacgtcg tgagacagtt cggtccctat ccgtcgcggg cgtaggaaat ttgagaggat
     4741 ctgctcctag tacgagagga ccagagtgga cttaccgctg gtgtaccagt tgtcttgcca
     4801 aaggcatcgc tgggtagcta tgtagggaag ggataagcgc tgaaagcatc taagtgcgaa
     4861 gcccccctca agatgagatt tcccatgatt ttatatcagt aagagccctg agag
//
LOCUS       DAUELZ010000104         4717 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.105, whole genome shotgun sequence.
ACCESSION   DAUELZ010000104 DAUELZ010000000
VERSION     DAUELZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.105"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            2..1198
                     /locus_tag="VHI09_001471"
     CDS             2..1198
                     /locus_tag="VHI09_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ6121334.1"
                     /translation="MKKRILSAVLVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTE
                     QKAAQNQVSALQAQVSSLQSEQDKLTARNTELEALSKRFEQEIKALTSQIVARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEE
                     KQAANQTAINTIAANMAMAEENQNTLRTQQANLEAATANLALQLASATEDKANLVAQK
                     EAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPAAPSARPQTSYDSSNTYPVGQCTWGAKSLAPWAGNNWGNGGQWAYSAQAAGYR
                     TGSTPMVGAIAVWNDGGYGHVAVVVEVQSASSIRVMESNYSGRQYIADHRGWFNPTGV
                     TFIYPH"
     gene            1451..2413
                     /locus_tag="VHI09_001472"
     CDS             1451..2413
                     /locus_tag="VHI09_001472"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ6121335.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMGIQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            2599..3354
                     /gene="recO"
                     /locus_tag="VHI09_001473"
     CDS             2599..3354
                     /gene="recO"
                     /locus_tag="VHI09_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEQ6121336.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            3457..4464
                     /gene="plsX"
                     /locus_tag="VHI09_001474"
     CDS             3457..4464
                     /gene="plsX"
                     /locus_tag="VHI09_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEQ6121337.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMKKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            4457..4699
                     /locus_tag="VHI09_001475"
     CDS             4457..4699
                     /locus_tag="VHI09_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ6121338.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHIAIPDEDVEDMVFMEDVLDYLVQRLT"
BASE COUNT         1533 a          730 c          982 g         1472 t
ORIGIN      
        1 tatgaaaaaa agaattttat cagcagttct tgtaagtggt gttaccctcg gagcagctac
       61 aactgtagga gcggaggatt tgagtactaa gattgctaag caggattcta ttatctcaaa
      121 tctgactaca gagcaaaaag ctgcacagaa tcaagtttca gcgttacagg ctcaagtaag
      181 ttcactacaa tctgaacaag ataaactgac cgcaagaaat acagaacttg aggcactttc
      241 aaagcgattt gagcaagaaa ttaaggctct aacaagtcaa attgttgctc gtaatgaaaa
      301 attaaaaaat caagctcgta gtgcttataa aaacaatgaa acttctggtt acattaatgc
      361 acttttgaat tctaaatcaa tttctgatgt tgtaaaccgt ttagtagcaa ttaatagagc
      421 tgtctctgct aacgctaaat tgttagaaca acaaaaagct gataaagttt cccttgaaga
      481 aaagcaagct gctaaccaaa cagctattaa taccattgcc gctaatatgg caatggctga
      541 agaaaaccaa aatacattac gtactcaaca agctaatttg gaagctgcaa ctgcaaattt
      601 agctctccaa ttagcatctg ctactgaaga taaagctaat ttggtagctc aaaaagaagc
      661 tgcagaaaaa gctgctgctg aagccttagc acaagaacag gctgctaaag ttaaggcaca
      721 agaacaggct gcacaacaag cagcatctgt tgaagcagca aaatctgcta ttactccagc
      781 accacaagct actccggcag cgcaaagtag taatgctatt gaaccagctg cactcacggc
      841 tccggcagct ccttctgcaa gaccacaaac atcatatgat tcttctaata cttatccagt
      901 tggacaatgc acatggggag ctaaatcttt agctccttgg gcaggaaata attggggaaa
      961 tggtggtcaa tgggcttata gtgctcaagc agctggttat cgtactggtt caacgccgat
     1021 ggtaggtgcg attgccgttt ggaacgatgg tggttatgga catgtcgccg ttgtagttga
     1081 ggttcaaagt gcctcaagta ttcgtgtgat ggagtctaac tacagtggta gacagtacat
     1141 tgctgatcac cgtggttggt ttaatccaac aggtgttaca tttatttatc cacactaaca
     1201 tattatttag gttagaacag ttgttctaac ctttttgatt attcctattg tggaatagtt
     1261 ctgagagtat tgacgatata gttaatttat aagagagttg tttggccaat cgcttatgta
     1321 taatataaga gaagtatata aagtcgataa aagatatatt taatacattc tttaacggtt
     1381 tttcaatttc atttgataat tcacttgaaa agcgttaaaa tggtatagga ttaaaacttt
     1441 ggaggaaaaa atgtcttatt ctgatttaaa attgtttgct ttatcgtcaa acaaggaatt
     1501 agcagaaaaa gttgcgtctg caatgggtat tcaacttgga aagtctacgg ttcgtcagtt
     1561 ttcagatggt gaaatccaag taaatattga agaatcaatt cgtggtcatc atgtctttat
     1621 tttgcaatca actagctcac ctgtcaatga caatttaatg gaaattttga ttatggtcga
     1681 tgctttaaag cgtgctagtg ctgaaaaaat ttcagttgtg atgccttact acggctatgc
     1741 acgccaagac cgtaaagcgc gttctcgtga acctattact tcaaaattag ttgctaatat
     1801 gttagaagtt gctggtgtag atcgcttatt aacggttgat ttacatgctg ctcaaattca
     1861 agggttcttt gatattccag ttgatcactt gatgggtgca cctttaattg cagactattt
     1921 tgatcgtcat ggtttagtgg gtgaagatgt tgtcgttgtt agtcctgacc atggtggtgt
     1981 gactcgtgcg cgtaaattag cacaattcct ccaaacacca attgcaatta tcgataagcg
     2041 tcgtagtgtg gataagatga acaccagtga agtgatgaat attatcggaa atgtttcggg
     2101 caagaaatgt atcttgattg acgatatgat tgataccgca gggactattt gccatgcagc
     2161 agatgcgctt gctgaagcgg gggcaacagc tgtgtatgcg tcatgtacgc atcccgttct
     2221 ttctgggcca gcacttgata atattcaacg ttcggctatc gaaaaactaa ttgttcttga
     2281 tactatttat ctgccaaaag aacgtcttat tgataaaatt gaacaaattt caattgctga
     2341 tttggttgca gaagcgatta ttcgtattca tgaaaaacgt ccgttgtcac ctttatttga
     2401 gatgggcaat taagcttaac gaagcctgag ataatagatc tcgggctttt tgacttgaat
     2461 aaaatgtaag ggtaatgtgg tattaattat tgtgggatga actatgcaat tctaaaggat
     2521 tttgggtata ataagaaaga taacaacagt ttattgtaaa ctaagataaa aggtaagaga
     2581 aaaatacgac gaggtaatat gcaactaaca gaatcactag gcattgttct ttttaatagg
     2641 aattatcgag aagatgataa attagtcaaa atatttactg aagtagcagg taagcagatg
     2701 tttttcgtga aacatattag tcgttccaaa atgtcctcaa tcattcaacc actaacgatt
     2761 gctgatttta ttttcaagtt aaatgataca ggcttgtctt atgttgttga ctatagtaac
     2821 gttaacactt atcggtatat taataatgat atttttcgat tagcctatgc tagttatgtc
     2881 ttagcattag ctgatgctgc gattgcagat aatgaatcag attcgcattt gtttacgttt
     2941 ttgaaaaaaa cacttgattt gatggaagag ggcctagatt atgaaatttt gacaaatatt
     3001 tttgaaattc agatattaga tcgttttggt attagtctaa actttcatga gtgtgccatt
     3061 tgtcatcgca ctgatttacc gcttgatttt tcccatcgtt tttcagctgt actttgttct
     3121 gaacattatt acaaagacaa ccgacgtaat catttagatc caaatgttat ctacttgttg
     3181 agtcgatttc aaaaaatcac atttgatgat ttgagaacta tttcattgaa taaagacatc
     3241 aaaaagaagc ttcgtcagtt cattgatgag ttgtatcacg actatgtagg aatcaaatta
     3301 aaaagtaaaa cattcattga taatttagtt aagtggggag atattatgaa atgatagggc
     3361 ctctttcgtt tatgggaatt ggtttcagaa tatgctataa taaaaatact attaatagat
     3421 tgtgtaagaa cacaagatat aattaaagga gtggaaatga agagaattgc aatagatgct
     3481 atgggtggag ataacgctcc taaagctatt gttgagggtg ttaatcaagc aatagaagca
     3541 ttttcagata tcgaaattca actttacggt gatcagacaa agattaactc ctatcttatt
     3601 caatcagatc gtgttgctat tattcataca gatgaaaaaa ttatgtcaga tgatgaacct
     3661 gcaaaagctg ttcgccgtaa aaagaaggct tcaatggttt tagcagctaa ggcagtgaaa
     3721 gaaggaaaag cagatgccat tatttcagca gggaatacag gtgctctttt agctgtaggt
     3781 ttgtttgttg tggggcgtat caagggagtt gatcgtccag gattattatc aactattccc
     3841 acagtaaccg gattaggatt tgatatgttg gatcttggag ctaatgctga aaatacggca
     3901 aaacatttac atcaatatgc tattttaggc tctttctatg ctaagaatgt acgtgggatt
     3961 gctaatccac gtgttggtct attaaacaat ggtacagaag aaacaaaagg agatcccctt
     4021 cgtaaggcca cttatgaact tttaacagca gataatacaa tatcttttgt tggtaatgtc
     4081 gaggcgcgag aacttatgtc aggtgttgca gatgttattg tatcagatgg tttcacggga
     4141 aacgcagtgt tgaaatcaat agaaggaaca gctattagca ttatggggca actgaaacag
     4201 ataattaata gcggcggtat taagactaaa attggagcta gtctacttaa aagtagtctt
     4261 tacgagatga agaagactct tgactactct agtgctggag gtgcagtttt atttggttta
     4321 aaggcacctg ttgtaaagtc tcatggttct agtgatgtaa aagctatttt ttcaacgata
     4381 aaacaagtac gtacgatgct agacactaac gtggtcggac aattagtaga agaatttgct
     4441 aaggagactc aagtaaatga ctagacagga gatttttgaa aggctgatta acttaattca
     4501 gaagcaaaga agctatttga gtgtagctat tacagaacaa acacatttaa aaaatgattt
     4561 agcggttgac tctattgaac tagttgaatt tattattaat gttgaagatg aatttcatat
     4621 tgctattcct gatgaagatg ttgaggatat ggtatttatg gaagacgttt tagattattt
     4681 agttcagcgt ttgacttagt tagaataata aaaagag
//
LOCUS       DAUELZ010000105         4555 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.106, whole genome shotgun sequence.
ACCESSION   DAUELZ010000105 DAUELZ010000000
VERSION     DAUELZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.106"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            246..1091
                     /locus_tag="VHI09_001476"
     CDS             246..1091
                     /locus_tag="VHI09_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ6121339.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGNAKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            1341..2405
                     /locus_tag="VHI09_001477"
     CDS             1341..2405
                     /locus_tag="VHI09_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121340.1"
                     /translation="MNEAIIQLDHIDITFHQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKIQLSAEALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEQKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGPVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            2406..3098
                     /locus_tag="VHI09_001478"
     CDS             2406..3098
                     /locus_tag="VHI09_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121341.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSIFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(3152..4522)
                     /gene="brnQ"
                     /locus_tag="VHI09_001479"
     CDS             complement(3152..4522)
                     /gene="brnQ"
                     /locus_tag="VHI09_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEQ6121342.1"
                     /translation="MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIG
                     FCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGA
                     TSFSVGIAPILGDNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
                     VIASFVHPAGNYGDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEAT
                     KQFGAKTDKEVTKITLISGAIAILLLALVYIFVGRIGATSQSLFPFIDGSFTLHGNPV
                     NGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWA
                     TIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIG
                     LTFIPAIFDALLTLSQMTGLFHLPEDVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLIL
                     SKTKKS"
BASE COUNT         1537 a          756 c          924 g         1338 t
ORIGIN      
        1 aaaaattgca aatagcctta atcaaaatga gatgttaaag tgctttttga gaagtgaata
       61 ttgtaagaag tatgctttgg gttttagtcc tcaaatacca agttttttgt tgactaaaca
      121 aataaggatg gttagcatag catatagttt aaaactatgg tatatgctag ccattatcaa
      181 tttgaaaact atatctaatt aagatacaat aatgatcgta aacaaaatga atcgagggta
      241 acgctatgtc ttttaaaaaa tggattggaa tagtcggaat cgctttggca tcaactgtat
      301 tggttgcttg ttctgaaaaa caagatgata aaaacactct aactattggg gtgatgacaa
      361 aaacagaatc tgatcaagcg agatgggata aagttgaaga attattaaaa aaagacaata
      421 tcactttgaa gtacaaagaa tttacagatt actcgcaacc taataaggca gttgctaatg
      481 gtgaagtcga tattaatgcc ttccaacact ataatttctt aaataactgg aataaggaaa
      541 ataaggaaca tttggtggcc attgctgata cctatatcag cccaattaac cttttctctg
      601 gaaccagtca agatgggaat gctaaataca aatcagtagc tgatctgcca aatggtgctc
      661 aaattgcagt accaaatgat gcgaccaatg aaagccgtgc cctctatgtg ctgcaatcag
      721 ctggcctcat taaattgaat gtgtctggtg atcaattagc aaccattgcc aatatctcag
      781 agaacaaaaa gaaattagat atcaaggaat tagatgctag ccaaacggca cgtgctttag
      841 tatctgctga tgcagctgtt gtcaataata gttacgctgt tcctgcaaaa attgactaca
      901 aaacctctct tttcaaagaa aaagcagatg acaattcaaa acaatggatt aacattattg
      961 caggtcaaaa agattgggaa aaatcagaga aggctgatgc tattaaaaaa ctcatcaagg
     1021 cctaccaaac tgatgaggtg aagaaagtcg tggaaaaaac ttcaaatggt atagatgtat
     1081 ccgtatggta aattttagcc attgttataa caagagtgtg gtcgtgttta aaacacatgc
     1141 tttcttgcat gcttgaggaa gagtttacga ttaatttacc tttagaaagc tatttaagac
     1201 cttttccctt ttaggattta gaaaccttgg gaagactatt gcatgttatc ttaatagtca
     1261 tgattatgca ggcgatgaaa acattgctat cgtagattac tgtcgccata tcgtactaat
     1321 agaggagtta ttaaaatctt atgaatgaag cgattattca attagatcat attgatatta
     1381 cctttcatca aaaaaaacgg gttattgagg ccgttaaaga tgtcacggtt catattaatc
     1441 agggagatat ttatggcatt gttgggtatt caggtgctgg gaagtcaacc cttgtgcgtg
     1501 tgattaatct tctacaagca ccaacaaatg ggaaaatcac cgtagatgga gacgtaacct
     1561 ttgaccaagg gaaaattcaa ttgtcagctg aggcccttcg tcaaaaacga cgtgatattg
     1621 gcatgatctt ccaacatttc aatttgatgg cccaaaaaac agccaaagaa aatgtagcat
     1681 ttgcgctccg ccattcgtcg ttaagtaaga ctgagaaaga acagaaagtt atcgaactgc
     1741 tagaacttgt gggtttatca gagagagcag ataactaccc tgcccagtta tcgggtgggc
     1801 aaaaacaacg agtggctatt gcgcgtgctt tagccaatga tcctaaaatt ttaatttcag
     1861 atgaggccac gtcagcacta gatccaaaga ccaccaaaca aatcttggcg ctcttgcaag
     1921 aattaaaccg caaacttggc cttaccattg ttatgattac tcatgaaatg caaattgtta
     1981 aggatatttg taatcgtgtt gctgttatgc aaaatggtgt tttgattgaa gaaggtcctg
     2041 tccttgatat cttttcaaat ccaaaagaag ccttgacaca ggaatttatc acaacagcta
     2101 cagggattga tgaggcttta gaaaaaatta accaacaaga cattgttaaa catttgcctg
     2161 caaatgccct tctagctcag ttgaaatatg caggaacttc tacggatgag cctctactta
     2221 atagcattta ccgtcagttt gaagtgacag ctaatatttt gtatggcaat attgagattt
     2281 tagatcatat tccagttggt gacatgattg ttgtcttgga agggcaagct gaaaatattc
     2341 ttgcggctga gaaagccttg catgaagctg gtgttgatgt tagtatttta aagagaggag
     2401 cctagatgtc acagttaatt caaacttatt tgccaaatgt ttatgaactc ggatggtctg
     2461 gagatgctgg ttggggctta gcgatttgga atactttata catgaccatt gtgcctttta
     2521 ttgttggggg tgctattggt ctcttattag gcttgttatt agttttaaca ggaccggacg
     2581 gtgttattga aaacaaaacg atttgttggg tcattgataa agttacctct atttttagag
     2641 ccattccttt tgttattttg attgctattt tagctagctt tacctatttg cttttaagaa
     2701 caactttagg agcaacagcg gccttggtac ctttaacctt tgcgactttt cctttttatg
     2761 cccgtcaggt ccaagttgtt ttttcagaat tagacaaggg tgttattgaa gcagcgcaag
     2821 cttcaggtgc tactttctgg gatattgtca aagtgtatct cagtgaaggt ttaccagatc
     2881 ttattcgtgt ttctacagtc accttaattt ctttagttgg ggaaactgcc atggcaggag
     2941 ctatcggtgc tggaggatta ggaaatgtag cgatttctta tggttataat cgttttaaca
     3001 atgatgtgac ttgggtagcg actattatta ttcttttaat tatttttgct atccaattta
     3061 ttggtgatag cttgaccaga cggtttagtc ataaataaac gaaacaacac cctaagtaaa
     3121 aacttagggt gttgttgttt agatgatgtc attagctttt cttcgtttta cttagtataa
     3181 gccctattaa aaaaccaata gcagcaaata taatccatcc cattgagaat tgccctagtg
     3241 gaacagtttt ttggaaaaaa gttacaacgt cttctggtaa atgaaataat ccagtcattt
     3301 gtgatagtgt taggagtgca tcaaatatgg caggaataaa ggttagacca attgtagttc
     3361 gatagacaat aggatcatta ttgaagcatt tttgtgccaa aacaaggaaa attaaatcaa
     3421 ctgttaatgg gtataaaagg aataaaacag gagctgacca gttgataatg actgataagc
     3481 caccaaaata aaagaaagct gatagtaaag taaagatagt tgcccaagca atatgagata
     3541 aagcaggcac taatttatgg aagtattcag cgcttgacgt gattaagcct gttgaagtgg
     3601 ttagacaggc caggaaaatc acaacagcta gaaatgcttg tccgatgcca cctaggtaaa
     3661 aacgagaagc atgacttaag atttgaccgc cattaactgg attaccatga agggtaaagc
     3721 tgccatcaat aaaaggaaat aatgattgtg atgttgctcc aatacgaccg acaaagatat
     3781 agacaagtgc taatagcaaa atggcaatag ccccagaaat aagtgttatt ttggtcactt
     3841 ctttgtccgt cttagcgcca aattgtttgg tagcctcaat gactaaaata gcaaaaacaa
     3901 gagaagctag tgcatccata gtgccataac cttgaattaa tcctgctata aaaggaaaat
     3961 ctttaaaggc attattaaca ccgaccccag cgttaaaagc atcaccgtaa tttccagcag
     4021 gatggacaaa ggacgcgata accaaaatag aaataacaac taacaacgtt ggcgttaaaa
     4081 attttccgat attttcagct agcttactag gtttgatagc aaggaagtag gacagaccaa
     4141 aaaatagtat agcataaata gctttattgg ttgtattatc tcctaagata ggagcgatac
     4201 cgactgaaaa agaagtggcc cctgttcttg gaatagcaaa aaatggacca atagataaat
     4261 acaaaatgga agaatagaag attgcatacc atttagagat ggggcgtgcc aaactttcaa
     4321 catcgcctga tccagattta gcaacagcaa tgacaccgag taaaggcaag gagacaccag
     4381 ttaaacaaaa accaatgata gaccatagaa tctgatgacc tgagtagatg cctaaaaaag
     4441 ctgggtaaat gaggtttgct gctccaaaaa atagcgcaaa tagcatgaat ccaatgacga
     4501 tatatacatt tttttgtttc atttataaat agtctttcta ttgatactaa tccga
//
LOCUS       DAUELZ010000106         4493 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.107, whole genome shotgun sequence.
ACCESSION   DAUELZ010000106 DAUELZ010000000
VERSION     DAUELZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.107"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(293..694)
                     /locus_tag="VHI09_001480"
                     /pseudo
     CDS             complement(293..694)
                     /locus_tag="VHI09_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1117..1650)
                     /locus_tag="VHI09_001481"
     CDS             complement(1117..1650)
                     /locus_tag="VHI09_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEQ6121343.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            complement(1730..2506)
                     /gene="recX"
                     /locus_tag="VHI09_001482"
     CDS             complement(1730..2506)
                     /gene="recX"
                     /locus_tag="VHI09_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEQ6121344.1"
                     /translation="MKITKIEKKKRLYLIELDNDESLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIPDIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIENLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            2621..3976
                     /gene="rlmD"
                     /locus_tag="VHI09_001483"
     CDS             2621..3976
                     /gene="rlmD"
                     /locus_tag="VHI09_001483"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ6121345.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITSVKRNFAEAKLLMVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLARFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFTFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKIYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
BASE COUNT         1353 a          714 c          936 g         1490 t
ORIGIN      
        1 ctatttagta actttggtta aagtaatcga actaacttag ttgattgtat tttaatgata
       61 aaaagcttaa atttgttgta taacaaattt aagcttttta ttgtttcttt ttaattacag
      121 attgaggttt aaaagacact ttacaatgtt ctcaagtcat gcgttgttgt aggttgtatg
      181 cacatattac aaatcccatt ataaaagaaa ttgtttaatt aatggatagt aatggatggt
      241 atggagatct taatgtggtt ttaatggtaa ttttagcagt tatagtaaga caaaaagtga
      301 cttggaaaag cttatacaga cgattcttaa ctcccacaat atcttctgtc agattttatt
      361 agacttcttc ttggacatga gttggtttac gttttagtac aaattgagat tgagctagtg
      421 tttcagtgtc aattttgtat gatttatgaa cgtgcaggct attcaattgc ttcttagctt
      481 ctaaaggatt aaaccgcatt atctgcgaaa aaacctgaag acgacgagaa tagacattaa
      541 tcgctttaag gataatctca gggttatgga cggttttcaa gtttccaaga agacgattga
      601 ggccgatggt atcgttttgt cttgaatgat tccttatttt agtggtttca ttttcaatac
      661 acgacgtcaa gcgtcctaca tactttgata aaattctacc taaaagaggt gtcttgtcag
      721 ttttgattac aacatcaagt gccaaaagac tttgcgactt aacaagacac ttctacttta
      781 acgcaaagaa aaagtagtga gtactctctt ccgtcggaga tttcctcgct actaaactta
      841 gtattttttg atactttcct taggtagtgc ttaaggcagc gacttccttt ggaattccat
      901 gccttatttt tgagatccta ggctcaaaaa taccaagtgg cgtcaatgtt agtgttgacg
      961 ccacttttac cacaacgaaa aacatctgtt ttggctcact ccacacgcag aaataggaaa
     1021 ttggatgtca aatcagcaga gagctgttca tgctttttga gcctaaaact gagtttgtct
     1081 gcgttctgag cactccttgg gagtgctgga caactcttaa cgattcttca gttcaagata
     1141 acgtttgtac caaatagtga tataggcctt ggaaaatggt cctttttcat gattaatcca
     1201 atcaaccaat attttgacat tttcttttag gataaagtcc atatcggctg aatactgcat
     1261 ctctttttta tgaatctcgt attcatcaac atcaagtaat ctcttttcac catccgcaaa
     1321 gactttgaca tctaaatcgt aatcaatgta tttgagggcc tcagtgtcca tcatatatgg
     1381 actagctaag ttacagtaat aagagacgcc attatctcga atcatggcta taatgttaaa
     1441 ccagtatttt ttatgaaagt aaacgattgc aggttcgcgg gtcacccatc gtctaccgtc
     1501 actttctgtg acaagagtgt gatcattaac tccaatgagg gcattttcag ttgtttttag
     1561 taccatagta tcgcgccaag tacggtgcaa actaccatca tgcttataac tttgaattgt
     1621 aataaagtcg ccttctttag gtaatttcat gccttaccaa ctttctacaa ttaaacttat
     1681 cctctatagt ctatcataaa tgtgtgaaaa aatcacttct tttgtcagtc tataaataat
     1741 ttcttaactt gcaattaata tcgtcgctgt tgtagccttt tcggtagaga gcctgataga
     1801 gcttttgctt taaagtataa ccatcatatt tgcgactgag tttacgatat tgtttgtcta
     1861 attctttgtc aagaagattt tctatttctt gattatcttg gtcaaaatta aggtgattga
     1921 ggctatgttt agctagatcg tatgaaaagc ctttggtcag taatgcttgg gtgattttat
     1981 ctttcaaggc ttttggtggc aatttttctt gatatttgtc aaagagtttt tggcttactt
     2041 tttgagcgag ttggctaaag tcagtttgag ataagatagg atcaatgtca tgacttgcaa
     2101 tgcctttttg taataatttt tgttttaata cttggggacc tttatcacca tttaactgat
     2161 tttggcgaat gtaggtatca gccaatttgg tgtcgtctat ccattgttct tcttggagtt
     2221 gagtgatgat gtcaggaata atgtgttctt caatctcatg cttgcgcagg taatcagcaa
     2281 cttgcttgtt gctgcgttgt tgaaaggaaa gaaaataaag ggctaaattt ttgccgtagg
     2341 acagttgggc aaaatgtttc atgtcttcaa gctgatcatt gtcaaggact ttatctttac
     2401 tcaacataaa ccgaacaata gtatcttctg ttacataaag ggattcgtca ttatccaatt
     2461 cgataaggta caggcgtttt ttcttttcaa ttttagtgat tttcatactt tgattatatc
     2521 aaaaatggta aaatagaaga cgaaaatact gctaaaaatg tgttgcttgt gaatgctttt
     2581 ggtggtgaga ttaggatatt agaggaagta aggggaagag atggtcgtaa aggttaaaca
     2641 gaaaattcct ttaaaaatta agcgaatggg aattaacggt gaggggattg gtttttatca
     2701 gaaaactttg gtatttgtcc ctggtgcttt aaagggagaa gatatttttt gccaaatcac
     2761 gtcggttaaa cggaattttg cagaggccaa actgttgatg gtaaacaagg cttctaagaa
     2821 tcgtgtcaaa ccagcttgtt ctgtttatga aacttgtggt ggttgccaaa tcatgcatct
     2881 agcctatcct aaacagctag atttcaaaga tgatgtgatt aggcaggccc ttaaaaagtt
     2941 caaaccaact ggctatgaac aatttgagat tagaccgact ttaggaatga aaaagccaga
     3001 tcactatcga gccaaattgc agtttcagct gcggtcattt ggcgggacgg tcaaggctgg
     3061 tttattttcc cagggaagtc atcgcttggt tcctattgac aattgcttgg tacaggatca
     3121 actaacgcaa gatattatca ataagattac gcagttagtg gacaagtaca agttgcccat
     3181 ttacaatgag cgaaagattg ctgggattcg gacgataatg gttcgtaagg cacaggctag
     3241 tgatcaggta cagattattg ttgtgagtag caaagaagtt aggttagcga ggttcattgg
     3301 cgaattgact aaggcatttc ctcaggttaa aacggttgcc ttgaatagta atcgttctaa
     3361 atcgagtgag atttatgggg acgaaacgga aattctatgg ggacaagagg cgattcatga
     3421 agaagtttta gactatggat ttgcgttatc accgagagct ttttaccaac ttaatccaca
     3481 gcagacagaa gttttgtatg gtgaggtggt gaaggccttg gatgtaggga gcaaagatca
     3541 tattattgat gcctattgtg gggttggctc gattggcttt acttttgcag ggaaggtcaa
     3601 gtctgtgcgg gggatggata ttatcccaga agcgattgag gatgcacaaa aaaatgccaa
     3661 agccatgggc tttgacaacg cttactatga ggcagggaag gcagaggata ttattcctaa
     3721 atggtataaa caaggttacc gcgcggatgc catcattgtg gacccaccaa gaactggttt
     3781 agatgataaa ttattaaaaa cgattcttca ttaccagcct aaacagatgg tgtatgtgtc
     3841 ttgtaatacc tccactttgg cccgcgactt ggtgcagcta acaaagattt acgatgttca
     3901 ctatatccaa agtgtagaca tgttccccca tacagctagg accgaggctg tcgtgaagtt
     3961 acaaaaacgt gtatgattga gtgccaataa caactcaagt tttataagct caggtaatgg
     4021 gtttgagcaa tagcttgaca tagtagggat gtcaaaaacg gtttagtaag catagtcata
     4081 ttattgataa ctctatgttt attttagaaa cattcatggt taaaaaagtt tgttagtttg
     4141 tcactaagta gggtctattt gaagagcaag tacacacaga caatattagg gtaattggtt
     4201 gttgaaaaaa agctgttttt tgctataaat tagaaaactg agtggtcagg aggttacatt
     4261 accagaagtt attttagtat gctaatatga acgatgatat agaggattta tcctgattta
     4321 atcttttaag ctgtaagaaa cctttgcaaa aactgtaggc tgtgtgagtc ttgatgtgtt
     4381 agatattaaa gataaatgca acactttttt ataatgagat caacaactga taaatctaag
     4441 ttgataacca gtaaaatgat cggaatgtag aaaagttact taatcaagct ttt
//
LOCUS       DAUELZ010000107         4442 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.108, whole genome shotgun sequence.
ACCESSION   DAUELZ010000107 DAUELZ010000000
VERSION     DAUELZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.108"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            1..846
                     /locus_tag="VHI09_001484"
     CDS             1..846
                     /locus_tag="VHI09_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEQ6121346.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFD
                     GTLHFIPNRSITVVSNKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIV
                     GKPNILGPQNNSNGQFTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYS
                     ITSKS"
     gene            complement(911..1471)
                     /locus_tag="VHI09_001485"
     CDS             complement(911..1471)
                     /locus_tag="VHI09_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEQ6121347.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQLMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(1485..1955)
                     /locus_tag="VHI09_001486"
     CDS             complement(1485..1955)
                     /locus_tag="VHI09_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ6121348.1"
                     /translation="MQNSKIRQMSLTGILTALVVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGRKRWFGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            complement(1945..2709)
                     /locus_tag="VHI09_001487"
     CDS             complement(1945..2709)
                     /locus_tag="VHI09_001487"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEQ6121349.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHVGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            complement(2699..3448)
                     /gene="truA"
                     /locus_tag="VHI09_001488"
     CDS             complement(2699..3448)
                     /gene="truA"
                     /locus_tag="VHI09_001488"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEQ6121350.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            complement(3635..4216)
                     /locus_tag="VHI09_001489"
     CDS             complement(3635..4216)
                     /locus_tag="VHI09_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ6121351.1"
                     /translation="MHNEKNNRTLVSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKV
                     KPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYL
                     KDLLRRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQER
                     MQFQALIRGERFKGRRALLRKISPYFKEFAQQL"
     gene            complement(4212..4285)
                     /locus_tag="VHI09_001490"
     tRNA            complement(4212..4285)
                     /locus_tag="VHI09_001490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4249..4251),aa:Arg,seq:acg)
     gene            complement(4365..4438)
                     /locus_tag="VHI09_001491"
     tRNA            complement(4365..4438)
                     /locus_tag="VHI09_001491"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4402..4404),aa:Asn,seq:gtt)
BASE COUNT         1406 a          984 c          654 g         1398 t
ORIGIN      
        1 atgacattta tattacatta cctagagcag tctcatatcg aaaatattgg cttaacgatt
       61 tttaaaaagc tagtgtctct cattattcta ttactctttt ttgctatttt aaaacgtgtt
      121 actaactatc tttttgaaaa aaccattaac aaatcgtttg cttattcaag acaaagtgaa
      181 gctcgcaaaa aaacactctc taagctcact cacaacattt taaattactt attgtatttt
      241 cttctcattt attggatact tagcttattt ggcatacctg tctctagtct tctagcagga
      301 gctggaattg ctggagtagc aatcgggtta ggtgcacagg gatttttatc tgacgttgtc
      361 aatggctttt ttatcttatt tgagaatcaa tttgaagtag gagataacgt tactatctct
      421 gacatagaag gcagtgtctt tggtgttgga attagaacta ctcaaattcg tggattcgat
      481 ggaacactac atttcatccc aaaccgtagc atcaccgttg tcagcaacaa atctcgtggt
      541 aatatgcgtg ccttaattga aattcctctt tattccactg ttaatttgag ccaagtcact
      601 cgaatcattg atgaagtgaa tcaaaaggaa ttgccaaacc atcctcaaat cgttggcaaa
      661 ccaaatattt tagggcctca aaataactct aatggacagt tcacttttag aattgctatt
      721 ttcacagaaa atggagaaca atttaagatt taccatactt tttatagact ttatcaagaa
      781 gctttactca aagaaggcat tcaattaccg actgcaatca gctattctat cacctcaaaa
      841 tcttaatata atcttaaaaa atcctttttt ctagaaaaaa caccaattgt tttattccag
      901 aaaaaagggt ttaggattta cggataatat ccatattgta atcagcccta gctccaccaa
      961 ttaattttgg tcgactagcc aaagctgtca catgagcacc tcctagctct cgttgacaag
     1021 gaatcagagg gatctgaaca cgcttaatat gcattcctat agcagtatca cctatatcaa
     1081 gtccagcgtg tgcaataact tcttctactt cgacagggtc tgacataagt tgaaaagcag
     1141 ccatctgagc actgccacca gcatgaagat tcggaaccac attgacaatc tctaactgct
     1201 tagattctgc cacatgcctc tctactacca aggcacgatt gacgtgttca caaccttgta
     1261 cagccaaatg aacaccacgc ttatttaact catctaacac aacttcaaca actatttgac
     1321 ctacttctag actagattgt tttccaattc tactacccaa aatttcgcta gatgaaagac
     1381 ctagaacaaa aagattacct ggttgaatag cagagcgttc tacaacatca ataacgattt
     1441 cccttgtttg tttctctaaa ttattcaaca acttctcact ctccttatct ctttttttgc
     1501 cttaatatgg ccttaaagat aataaaacca acaaaaagtc ctaatgtatt ttgcatgacg
     1561 tttccccaaa taccggctaa agatcccgaa aggccataac caagcataag agagcctaaa
     1621 aaataccaaa aaatcatgat aaaagaacct atcacaacac caaaccaacg cttgcgtcct
     1681 ctccatcccg caaaataccc ttggacactg tgagcaatca aactatgaaa catccactga
     1741 gggtatcctg ccactaaatc gattaaaaaa cctgataacc ctcccacaat agctccttga
     1801 gctgatccaa aggaaaagct gacagcataa attcctgcat ccaacagcgt taaaaaacct
     1861 gttggagtag gtagcatgac aaatcttcct aaaacaacaa ccaatgctgt caatatccct
     1921 gttaaactca tttgtcgtat cttactattt tgcataactt tgttttactc cgtatctatc
     1981 agattgtaaa atagcttggt agactaactc ttttgagttt ttcacagctt ctagtggcgt
     2041 ttttttcttt actaactgac tagcgatact agatgcaaaa gtacagcctg cgccaatatt
     2101 atttttctca agtacaggac attccaaggt aacaatctct ttaccgtcat aaaaaagatc
     2161 aatagccttt ttctgactaa atcgattacc acccttgata accacctgtt ttgccccaag
     2221 ttgataaaaa tatttagcag cttcttgcat atcttttaaa gaaacaatct ctttttgaga
     2281 taatagctga gcctctacca aatttggagt aacaacagtt acataaggca ataattgtaa
     2341 aatctcctgt cttagaggaa ctattttaac atcatctatt tctttacatg ctagaacagg
     2401 atctaaaacc actgggattc ccacatgccc ctttataaaa tccaaaacta tttcacacat
     2461 ttccgcatta ggtaacaaac caattttaat agcagaaatt ggagcgttcg taaagctgtt
     2521 caactgatca cggaaaattt ctttagctac cggaaataga ctaaagccct cttcagatct
     2581 agtagttaaa caagttaccg ctacaaaggc ctgtaggtca taccttatgt aagtcgctaa
     2641 atcagcatag aggccacctc cacttaaaat atcgttccca gatatagtca cgatataatc
     2701 agttttcata acaaatctcc ttcaagtaaa gcccatttcc agatatcgtt ggtccagcta
     2761 gctgacgatt tttggagctc aaaatcactt taacttgttc aactggcatt tgaccattac
     2821 caattttcag caaagttccc accatattcc tcacctgctt atagagaaaa ccatttcctg
     2881 aaaaagtaaa caccaaaaaa tcagtttttt catcccgaga taccgttgct ttggtaatcg
     2941 ttcgcacctt attttgtacc gaagttcctg ctgctgtaaa accagtaaaa tcgtgtgtgc
     3001 ctaccagacc gttaatagct tcttgcataa gtttaatatt aagagtataa gggtaatgag
     3061 tggtatagtg tcgcatcatc ggatttttag gtcgaccatt atcaactaaa aattcataag
     3121 tttttaaatg cttctgataa cgacaatgaa aatcatcagc cactttttct atattaacaa
     3181 catcaatatc ttcaggagtc tgtgtatcaa gagcaaatcg taatttttca acttcttgtt
     3241 cttgaggcaa atcaaaatgg atcacctgtc cataagcatg tactcctgca tccgtacgtc
     3301 ctgcaccatg aattataatt tttgtaccgt tatttaattt atagagtgtt ttttcaatct
     3361 cttcttgaac cgtacgtaaa tgtctttgtc tttgaaaacc cgaaaaaaga gtcccatcat
     3421 aggaaatcgt tgctttgtat cttaccatgt tactatttta acatttctta aacgtaagag
     3481 aaactatttt taatcaaact gtacccaaac aaatgaaaga cgtattgaag ctaactctaa
     3541 gctactaacg atttaaaagc tataagaaag ctatttcctc caaactctct ctcctttttt
     3601 tatagattta cccccaattc cttataggtc ttgcttacaa ctgctgtgca aattccttaa
     3661 aatagggact gatcttccta agtagagcac gtcttccctt gaaacgttcg cccctgataa
     3721 gtgcttgaaa ctgcatacgc tcttgcgaat ttaattggtt ctctaagcta gctattacct
     3781 cttgataagc cacatagtcg tctagccata accctctcga tggaatggca tgtgcaacct
     3841 cccctatctc ttcatatgct aacttatgga actgacgctt ttgactttct tgacggcgta
     3901 ataaatcttt caaataagat gaaaattttg ttttaaaata gcgatataaa cgctcttctt
     3961 cctcaattaa ctctgggtac ctttctagta actgtaataa gataatatgt ccttcttgta
     4021 accagtcatc tctatcccac aattgaatat aatagtgtcg ctttaatttc aaaataatgg
     4081 gcttaacttt ttcaaacgct gctcttgtct ctatcgacat tttgagtctc cttttctttt
     4141 taactatccc tagtataaag agaaccacaa aaaaacaaca ggacactaat gtcctgttgt
     4201 ttttttcatt atgcacccta gaggagtcga acctctaacc gcctgattcg tagtcaggta
     4261 ctctatccag ttgagctaag ggtgccaacg catatcttgc ctctatctta ccacactctt
     4321 ctctaaaatg caaaaaaaga taggacgctt cctacctatc tctactccgc cagtaggact
     4381 cgaacctacg acatcatgat taacagtcat gcgctactac caactgagct atggcggata
     4441 aa
//
LOCUS       DAUELZ010000108         4434 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.109, whole genome shotgun sequence.
ACCESSION   DAUELZ010000108 DAUELZ010000000
VERSION     DAUELZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.109"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..244)
                     /locus_tag="VHI09_001492"
     CDS             complement(<1..244)
                     /locus_tag="VHI09_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121352.1"
                     /translation="MATELIFGVGGFILAIVTTYNIFNAKSIKHATDITLLQSEVEHL
                     KIVTRQNARRLEEHDEQNKTLITMTEQIKNLNREVRE"
     gene            complement(254..661)
                     /locus_tag="VHI09_001493"
                     /pseudo
     CDS             complement(254..661)
                     /locus_tag="VHI09_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(691..1947)
                     /locus_tag="VHI09_001494"
                     /pseudo
     CDS             complement(691..1947)
                     /locus_tag="VHI09_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            complement(1949..2098)
                     /locus_tag="VHI09_001495"
                     /pseudo
     CDS             complement(1949..2098)
                     /locus_tag="VHI09_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            complement(2370..2552)
                     /locus_tag="VHI09_001496"
     CDS             complement(2370..2552)
                     /locus_tag="VHI09_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121353.1"
                     /translation="MEKLTLKKEKENTATLPFFVGIDLHAQIKEISTETGIPMRRIIE
                     RMIRFSLDNLEIMEGD"
     gene            complement(2540..2752)
                     /locus_tag="VHI09_001497"
     CDS             complement(2540..2752)
                     /locus_tag="VHI09_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121354.1"
                     /translation="MDQYLINDNEFLRDENTRLNKEFSELNHAVAKANLLDVIIEDGY
                     ILQSTLDKCTAKLDEIDQKELKTWKN"
     gene            2837..3313
                     /locus_tag="VHI09_001498"
                     /pseudo
     CDS             2837..3313
                     /locus_tag="VHI09_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527534.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
     gene            3464..>4434
                     /locus_tag="VHI09_001499"
     CDS             3464..>4434
                     /locus_tag="VHI09_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ6121355.1"
                     /translation="MRKVAIYSRVSTINQAEEGYSIQGQIEALTKYCEAMEWKIYKNY
                     SDAGFSGGKLERPAITELIEDGKNNKFDTILVYKLDRLSRNVKDTLYLVKDVFTANNI
                     HFVSLKENIDTSSAMGNLFLTLLSAIAEFEREQIKERMQFGVMNRAKSGKTTAWKTPP
                     YGYRYNKDEKTLSVNELEAANVRQMFDMIISGCSIMSITNYARDNFVGNTWTHVKVKR
                     ILENETYKGLVKYREQTFSGDHQAIIDEKTYNKAQIALAHRTDTKTNTRPFQGKYMLS
                     HIAKCGYCGAPLKVCTGRAKNDGTRRQTYVCVNKTESLARRSVNNYN"
BASE COUNT         1309 a          893 c          795 g         1437 t
ORIGIN      
        1 gttctcttac ctcacgattg aggtttttaa tttgctctgt cattgtgatg agcgttttgt
       61 tttgctcatc atgctcctca agacgcctag cattttgccg agtgacaatt tttaaatgct
      121 ctacctcaga ctgcaacaaa gtaatatctg tcgcatgctt gatggatttt gcattaaaaa
      181 tattgtaagt cgtgacgata gctaaaataa agccaccaac gccaaatatc aactctgttg
      241 ccataaacca cctctaatct tgtttaacca aatcagcgta cttgataact gttactttgg
      301 cttctgactc tagctcctct aaggtttgtt tgtcatactc aaatgcttcg ttaacgtgta
      361 cgaagactag gttcccttcg cctgcttcac cgtcttcctc tttagtactg tcgaccaccg
      421 taaagacatc atacgcttga tactcacctt ttttggctgg ctcgattagc tcaagcatgc
      481 ctttataaat gtcagaatca atcttgccgc cgctcattaa catgtggatc gtttgtaagt
      541 taatcatctt ttgcgtgcgc tctgcggaca ccttagctag tccagcagct gtttgagcag
      601 ttttggcagt cttagcggtt tcctgtgaga ttttttcaag gtcgtctagt tccgttaaac
      661 tcaatgtatc tcactaagct aacctccctt agcggtatta cgtgataaaa ctttcaacgt
      721 ttcaccggat ttttcggtat agtaagccat tttgcgataa gcgtcatcat agctaatgcc
      781 aagcttctga gccatatctg acgccatttg acttatgccg cggtggattt cctcttgact
      841 tttaccagat tcttttaacc gttggactcg ctccctctgg atagcaagat actgatcatt
      901 caaagctttg gtttttaggt tgtgctgcat attaagttcg gtaagtctat tcaaaacttc
      961 ctgtctagca gccaactctt cagcactcaa tttactagtg tcgctgccaa attgcttcaa
     1021 aatctctttt aactctgctt tttttgtttt atatgcagaa ttttctttat cgagcatctt
     1081 aacagtgttt tcagatactt tttccaactg ccttgcattt agcgcatcta actcgttatt
     1141 caaagctgct gttaaagctt tcttttctga tgcagaaaac ttcattaaat caagttgagc
     1201 aataatcatt tctctctgat ttcgcaaaac aagttctttt tcagcgcttg acaactggct
     1261 catgtcacca ttgtgcttct ccatgattgc tttaatctgg gtgttcatgc ttttaacatt
     1321 gttaactact gattcagttt tggatttcaa agcatttatg gtttcttctg acaatcctaa
     1381 ttttttagct aagtttatat tcttatctaa ttttgattga gctaactttt caatacttcc
     1441 gaccaaatca tcaaaagctt cagtcacttt cttaacgcta cctgcgcctg cctcgaacat
     1501 ttgcaaagaa gtgttagttt cgtcaacttt tttcttaaag ttacttagtg cagttgcttc
     1561 tgtcgcggaa acagcggttc cccactcttc cgtgcgtttt tgggctttat acatgctatc
     1621 agcaattaaa ccaataccaa taacagctgc gccacctagc aaaacacccc acgttaccgg
     1681 gttgcttaat aaggcaaccg cgccagtcaa actgcccata gaagctgtgg cagcttcagc
     1741 tccaacactt gctgctactg caccggcttt aaacgtgcgt agccaaccag ataaagcacc
     1801 aatccctttg ctcatcttgc cgatagcttg aatggttccg ccgataacac caataccctt
     1861 acccaaaata gataaagccg gcccagcggc ggctgcaata agtccccact tgataatttg
     1921 ctgttgttgc tctttgtcga gcgagttatt ttaagtgtgg tgggtcgaag acaacaaggt
     1981 taaagctatt actttcaaaa ggcatatcac gaaaatcacc aattacatca gggttgacat
     2041 tgattttttt gccgtgcatc tcaaattttt cttgtctgac gtccataaaa gttgtgtgag
     2101 gttcgccgac ttcttgttct atggcttctg ttaattttcc agttcttaat tggatagttg
     2161 tatttgatat accaccgtat ctatcattgt aatctggtgc gataatcatt tttttatgtt
     2221 tgttaatgta gattctcaat cgtaagccct cgcttcccat gtgatttgcg gatcgggttc
     2281 tttacagcaa gggcattcca agtccttgta tgctgatatg tttgaccatt ttttgccgca
     2341 attttcgcaa taatattcaa agctatacat taatcccctt ccatgatttc taggttatca
     2401 agagaaaacc taatcattct ttcaataatt cttctcattg ggatacctgt ttctgtcgag
     2461 atttctttga tttgggcgtg taaatcaatc cctacaaaaa acggtaatgt tgcagtattt
     2521 tctttttctt tttttaaagt tagtttttcc atgttttcaa ctccttctga tcaatctcat
     2581 caagcttagc agtacatttg tccagcgtgc tttgcaggat atatccatcc tcgatgatga
     2641 catctaataa atttgcttta gcaaccgcat gatttaactc gctaaactct ttgttgagtc
     2701 gtgtgttttc gtctcttaaa aattcgttat cattgattag gtattgatcc attctttcct
     2761 cctaccaaat ttgtttagca ggcaaaccgt gtttttggtt atatctacgt gtattggctt
     2821 cccaaccgtt gttttcaaag aagtaaaagc tttgccagaa attttgtcag atgacgaaat
     2881 tatccaatac gcaacatctg ggtttgtaaa cggcaatact gtccttgttg tattgaccca
     2941 aaaacgaatt ttatttatcg ataagggaat gttatatgga attagatcga cagaaattcc
     3001 tcttgatatg gttaatagcg tctcatactc taaaggttta attcttggcg caatagctgt
     3061 tatgaacgga gccacaaaaa ttgaaatatc taatattgcc aaggacactt gccctgtact
     3121 taccgaaaaa ataaaagatt gtgtagaaca atacaaatat aatctgtacc aacatcaatc
     3181 gcctcagaaa acagcaactg taggctcgga agatgccgtt gctgaaatcc gtaagtttaa
     3241 aaaactttat gatgaaggca tccttacaga ggaagagttt atagctaaga aaaaaataat
     3301 tttagggatt taaggtgtaa agttaaccgt taaaaaataa aaaagcccca cgctctcaaa
     3361 ctttggcgag tctgagcgtg aggcgaattc tagtatagta aaaacctgct ttaagtaggt
     3421 ctctttactg tactcatttt aacaaaaaat gaggtaaaaa acaatgagaa aagtagctat
     3481 ttactctaga gtatcaacaa taaatcaagc cgaagaagga tattccattc aaggacagat
     3541 tgaagcttta acaaagtatt gtgaggcaat ggaatggaaa atttacaaaa actactctga
     3601 tgcaggtttt tcaggcggta aacttgaacg accggcaata acagaattga ttgaagacgg
     3661 taaaaacaat aaatttgata ctattttagt ctataaacta gacaggctat caagaaatgt
     3721 aaaagacaca ctttacttag tcaaagatgt atttactgct aacaatatcc attttgtcag
     3781 cttaaaagaa aatatcgaca cttcttcagc aatgggaaat ctctttctca ctctcttgtc
     3841 agctattgca gagtttgaaa gagaacagat taaagagcga atgcagtttg gtgttatgaa
     3901 tagagcaaaa tctggaaaaa caacggcttg gaaaacacca ccttatggat atagatacaa
     3961 caaagacgaa aaaacattgt cggtcaacga gttagaggct gctaatgtca ggcagatgtt
     4021 tgacatgata atctctggct gctcaatcat gtcaattaca aattacgcga gagacaattt
     4081 cgtcgggaat acgtggacac atgtaaaagt aaaaagaata ttagaaaacg aaacatataa
     4141 aggattagtg aagtataggg aacaaacttt ctcaggtgat catcaagcca ttatcgacga
     4201 aaaaacatat aataaagcgc aaatagcttt agcacatagg acagatacga aaacaaatac
     4261 tagacctttt caagggaaat atatgctttc tcacatagca aaatgcggtt attgtggtgc
     4321 ccctttaaaa gtatgcacag gaagagctaa gaacgatggt acgagaaggc aaacttacgt
     4381 ttgtgttaat aaaacagaaa gtttggctag acgaagtgtt aataattata ataa
//
LOCUS       DAUELZ010000109         4418 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.110, whole genome shotgun sequence.
ACCESSION   DAUELZ010000109 DAUELZ010000000
VERSION     DAUELZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.110"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            5..2278
                     /locus_tag="VHI09_001500"
     CDS             5..2278
                     /locus_tag="VHI09_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEQ6121356.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQTLASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEELSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     SLQSLSQEDQSKQLEQLKEAVAQIANQSNQALPGASSALTELSTGLAKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTAIGSGVIKLSEGTNALSSKSGELLDGSHQLSEGATKLADS
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(2394..3473)
                     /locus_tag="VHI09_001501"
     CDS             complement(2394..3473)
                     /locus_tag="VHI09_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEQ6121357.1"
                     /translation="MKNISRKCFITSVLCIILGGILLGAGYATGGLQDIKHQTAPKKV
                     IKTFDQITALDIDSSASTITVETGPVQRPTVTYYTHPKFIDPIVTTVTGKTLSLSQKP
                     KDVVITGGIEILGFTLNNSRQEKNYRSITITVPEKTSLNEVKGSNVPHTTLSNLTVQD
                     MQFDGNLTLLHTKVKKATITGMLEATKSQLTNLELKADYSFSNLTDSSVENGTISLGN
                     GQLTTKDTTLKAVNIQSLHSGGIEAERTTLENVTFTVSKSKEEEENDYYDNDAIFTAH
                     ALTLKGTNTITGGDIDVDITLTKAKAIAYRARTENGKVSLGSQLTPAKIGKESTSDVI
                     SYVAENKAATGNLTVNLNKGDITIK"
     gene            complement(3470..4057)
                     /locus_tag="VHI09_001502"
     CDS             complement(3470..4057)
                     /locus_tag="VHI09_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEQ6121358.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEAMEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGLAGILVAGVTLFESLTLIGSSIPAQAMGIGSALLCFG
                     GGLLIWIIATAILRFSGRAFVAFIKWISNKKGAQA"
     gene            complement(4044..4370)
                     /locus_tag="VHI09_001503"
     CDS             complement(4044..4370)
                     /locus_tag="VHI09_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEQ6121359.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
BASE COUNT         1318 a          846 c          894 g         1360 t
ORIGIN      
        1 aaatatgtta gaagaattga aaacacttat taaaaatcca aaattaatga ttacaatgat
       61 tggtgtggcc ctagtgcctg ccttatataa tttatccttt ctaggctcaa tgtgggatcc
      121 ttatggtcgg gtcaatgacc ttcccattgc tgttgttaat catgataagc ctgcaaagag
      181 agctgataag tcattgacaa ttgggaatga tatggtggac aagatgtcta aaagtaaaga
      241 tttagagtat cattttgtct cagctaagca agctcaagag ggacttaaag aaggtgatta
      301 ttatatggtt attactttac ccgaagatct ttctcaacgg gcagcaacct tattaaatcc
      361 cgaaccccaa aaattaacta tccgttacca aacgagtaaa ggacatggaa tggtcgctgc
      421 taagatgggg gaaacagcga tggctaagtt gaaagagtct gtttcgcaaa acattacgaa
      481 gacttatacc tcagcagttt ttagcagtat gacagacctc caatcaggat taaaagaagc
      541 ctcagctgga agtcaaacac ttgcttcagg agcgaagacc gctcaggcgg ggagtcaaac
      601 actttcgacg aacttagcag ccttaacggg tgctagccaa cagtttcaac aaggtactgg
      661 tcgattgaca tcaggtttga ctacctatac agatggtgtc aaccaagtca agaatgggtt
      721 aggaacatta tcaacggaca tccccaatta tctgaacggg gtttctaggt tatcccaggg
      781 agcttctcag cttaatcagg gtctttcaca gttgacacaa gcaacaacac tttctgacga
      841 gaaagctaag ggaattcaat ccttaattgt aggactacca gtcctaaatc aaggcattca
      901 gcaactaaat acagagctat caacattgca accccctaac cttaatgctg atgagttagg
      961 taatagctta ggagctatcg ctcaagctgc caaacaagtc attgctgaag agactgccgc
     1021 tcagaatgaa gaactatcgg ctctccaagc tactagcgtt taccaatcat taactgctga
     1081 acaacaagga gagttagctg cggccctcag tcaatctgat aaaagtcaga ccgtatctgc
     1141 agcccaaact attttaagtt ctgttcaaac tttgtcaaca agtttacagt ctctctctca
     1201 agaagatcag tcaaaacagt tggagcaact taaggaagct gttgcacaga ttgctaatca
     1261 atccaatcaa gctttgccgg gagcaagttc tgctttaact gaattatcaa cgggattagc
     1321 aaaggtaaat ggtagcttaa atcaacaagt tctaccagga agtaatcaat tgacaacagg
     1381 attagcacaa ttaaacaggt ataatactgc cattggttct ggggtaataa aactctcaga
     1441 aggtaccaat gccttgtcat ccaagtccgg agaattacta gatggtagcc atcaattatc
     1501 agagggtgct actaaactag ctgatagtag ttctcaattg agtcagggtg gtcatcaatt
     1561 aacgagcgga ttgactgaat tatcaacagg attatcaacc ttaaatggtt ccttagccaa
     1621 agcctctcag cagttatcgc ttgtttctgt gactgataaa aatgctaaag ctgtcgcaaa
     1681 acctcttgtg ttaaatgaga aagacaaaga tggtgttaag acgaatggga tcgggatggc
     1741 accttatatg attgctgttt ctctaatggt tgtggccctt tcaaccaacg tcatttttgc
     1801 taattcttta tctggtcgtc cggtcaaaga taaatgggat tgggctaaac aaaaatttgt
     1861 tattaatggt tttatttcga ctatgggatc cattgttctc tacttagcta ttcaattatt
     1921 agggtttgaa gcccgttatg gtatggaaac cttaggattt attatgctaa gtggttggac
     1981 gtttatggct cttgtcacag ctttggtcgg ttgggatgat cgatatggct cttttgcttc
     2041 tttggtcatg ttattgcttc aggttggctc ttcaggtggc tcttacccca ttgagttaag
     2101 tggagcattc ttccaaaagt tacatccttt cttaccaatg acttatgtgg tatctggttt
     2161 acgacaaacc atttcattat caggtcatat tggagtagaa gtgaaagtct taactggttt
     2221 cttactggca tttatggtat tagcactact catttatcgt cctaagaaaa cagtctaaat
     2281 aaaataacct caaaaaaaac agcatctaag gcagttacac tagatgctgt tttttgtctt
     2341 attttgacaa taacttgaca atttctgtca agttttttat tgtcttgtta agatcatttg
     2401 atagtaatgt ctcccttatt gagattaacc gttaaatttc cagtagctgc tttattctca
     2461 gccacataag aaataacatc tgaagttgat tccttaccaa tcttagctgg tgtcagctgt
     2521 gagccaaggg agactttacc attttcggtt cttgctctgt aggcgatggc ctttgctttt
     2581 gtcaaggtta tgtcaacatc aatatcacca ccagtaatag tattagtccc tttaagggta
     2641 agggcatgag cggtgaagat agcatcattg tcatagtagt cgttctcctc ttcttctttg
     2701 cttttagaaa cagtgaaggt cacattttca agggttgttc tctcggcttc tataccacca
     2761 gagtgtaatg attgaatatt gacagctttt agagtggtat ctttagtagt tagttgtcca
     2821 tttcctaagc tgatggtccc attttccaca ctagaatcag tcaggtttga aaaagaatag
     2881 tcagctttta actcgagatt tgttaactga cttttagtgg cttccaacat accagtgata
     2941 gtagctttct tgactttggt atgtaaaaga gtaagattgc catcgaattg catatcttgg
     3001 acagttagat ttgacaaagt ggtatgtggg acattacttc ccttaacttc attgaggcta
     3061 gttttttcag gaacagtaat ggtaatagaa cggtagttct tctcttgacg actattattg
     3121 agagtaaaac ctaaaatttc aattccacca gtgatgacaa cgtctttagg tttttgcgaa
     3181 agagataggg tcttacctgt tactgttgtg acgatagggt caataaattt aggatgtgtg
     3241 taataggtca ctgttggtct ttgaacgggt cctgtctcta ctgtaatagt tgaggcagaa
     3301 ctgtcaatat caagagcagt tatttggtca aatgttttga tgaccttttt gggagctgtt
     3361 tggtgtttaa tgtcctgaag tcctccagtt gcatagccag cccctagaag aatgcctcct
     3421 agaataatac ataacacact ggttataaag catttgcgtg agatattttt catgcttgag
     3481 ctcctttctt attactgatc cacttaataa aggcaacaaa agcccgtcct gaaaaacgaa
     3541 ggattgctgt ggcaataatc caaataagaa gaccaccacc aaaacacaac aaggcgctgc
     3601 cgatgcccat agcttgtgca ggaatagatg agccaatcaa ggtcaagctt tcaaacagag
     3661 tcactcctgc gacaagaata cctgcaagcc caaaaatgta ggcggcaaag atagctgtga
     3721 gcccgagcat taggcaggca ataataaaga gaatcaacgc cagcaatacg ggtaaagcaa
     3781 tcggagcagc aaagagggac aaaatggtta atccaataat tgttgcccga tttttgggag
     3841 ttttctcggg agttgccaag tgttttccta gaacatttga aatgatttca ctggcagctt
     3901 ccttaggatt tcctaaatcg tcgataacct ggctttcttt gtcaggacca gcttcatcaa
     3961 aatattcaat ataatattcc atcgcttcat ggtagtcttc tctaggtagt ttgcgcaagt
     4021 atttatctag ttctgctaga tactcagtcc ttgtcatgtc ttaagctccc ttcaataatc
     4081 ccgtctaagg caaatttata gctctgccat tcttttttta gaaaaatcag ctgtgctctc
     4141 cccgaagaag tgacagcata atatttacgc tttcgtccct gatgttcttg actataagtt
     4201 gtcaaaaaac ctgctttctc caatttttta agaataggat agagagtgga ttctttgata
     4261 ttagccacta gtttaatggt ttgactaata tcgtaaccgt aagaatcatt tttttcgaca
     4321 atggctaaaa tcagaaattc aatcaaggta gccgatactg gaaaatacat tttaggctcc
     4381 tttcatatat aaaaatttta tatataatta ttttacat
//
LOCUS       DAUELZ010000110         4126 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.111, whole genome shotgun sequence.
ACCESSION   DAUELZ010000110 DAUELZ010000000
VERSION     DAUELZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.111"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(150..2414)
                     /gene="glgP"
                     /locus_tag="VHI09_001504"
     CDS             complement(150..2414)
                     /gene="glgP"
                     /locus_tag="VHI09_001504"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEQ6121360.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNTA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNYWIEDDSWLVPTDI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNKDIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     RLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
     gene            complement(2449..3942)
                     /gene="malQ"
                     /locus_tag="VHI09_001505"
     CDS             complement(2449..3942)
                     /gene="malQ"
                     /locus_tag="VHI09_001505"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEQ6121361.1"
                     /translation="MNKRASGILIHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEAADLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
BASE COUNT         1243 a          800 c          728 g         1355 t
ORIGIN      
        1 tccatagatc gataaacaga cttgctttac gcttaatgta ataattaacc taagtctgaa
       61 gtaaaatcat aaaacagcag aaactactgt cttataattt agtttaagca tgttacggta
      121 ccgctacccc acattgcaat tattattttt tatcgtgaat gccaaatatc ttcgttatac
      181 tgttcaatgg tacggtcaga tgagaagaat ccagctttgg caatgttgtg aaccactttt
      241 gtcatccaca aatcttggtc ttcgtagtct gctaacattt tttctttcac ttcaatgtac
      301 tcttcaaggt caatcaaagt catgaaccag tcttttgaaa tcaattcttt ataaagacga
      361 tcaagacgtt cttcgttgcc aaatgctagc aattctggac tgataataaa gttcactgct
      421 gctttaatag cagggtggtt atcatagtaa tcttttgcta cataagaagc agtcgcataa
      481 aggttgatga tggtgtcaga atctttacca aaggtataga tattctccat gcctgcaagc
      541 tcagcgattt ctacgttagc accgtccatt gtaccaagtg ttaaagcacc gttaagcatg
      601 aatttcatat taccagttcc agaagcttct ttggatgcta gtgaaatttg ctcagaaata
      661 tcagttgctg gaatcaagtg ctctgctact gtcacgttgt aattttcaac tagatgcaca
      721 ttaaggtatg ggcttacttc agggtcattg ttaatcaatt cagacaagca aaggatcaag
      781 tgaatgatgt cttgagcaat gatgtaagca ggcgctgctt tacctccaaa gataacagtg
      841 attttacgtt ttggaaggtt gcctttttta atttccaaat atttgtgaat cacgtaaaga
      901 gcattcatct gttgacgttt gtactcgtgg aaacgtttga tttgggtatc aataatagaa
      961 tgttcatcaa gctcaatgtc tttattgtct ttaaggtaac gtttaagagc taatttgttg
     1021 ttgaatttga tttcagccaa tttagcatga acagctttgt catctgcaaa ggccatcaat
     1081 ttttctagtt ttgttgcgtc agtcaagtac tcatcgccaa taagttcttt aatgtaatca
     1141 gccaagtctt ggttagcaaa ttctagccaa cgacggaaag taatcccgtt ggttttgttg
     1201 ttgaattttt ctgggtaaag gtcatagaaa gcttttaatt cgctgttttt caagatttct
     1261 gtgtggagtg ctgctacccc attgacactt gttgcaaaat gaatatccat atgggccatg
     1321 tgcacacgac ctgattcgtc aataatttga acagctgggt cagacacttc tgaacgaaca
     1381 agagcatcca atttttcaat gatggttacc aagtgtggca ctacttcgtt taagtaagca
     1441 gttggccatt tttcaagggc ttctgcaaga atagtgtggt tagtgtaacc aaccatattt
     1501 ttcacaacag ctaccgcttc atcaaagtca aagccatgtt tttcagtcaa gagacgaatt
     1561 aattcaggaa taaccattga tggatgcgtg tcattaattt gcacgtaagc gtagtctgca
     1621 aggtcatgaa ggtttgaacc acgttcgata gcttcatcaa tgattaattg cgctgcattt
     1681 gacaccataa agtactgttg gtaaatacgg agcaattccc catttttgtc agaatcgtct
     1741 gggtagagga acaaggtcaa gtttttagca atttgcgttt tatcaaatga aataccgtct
     1801 ttgattaacc cgtaatcaac gccctcgata tcaaacaagt taaggtagtt tttagtgtca
     1861 cgtttgtaac ccaaaacatc aatacgatca agacgagatt ttaaggtaaa gtttttgaag
     1921 ggcacatcgt aagaaatgtc tgttggaacc aaccatgagt catcttcaat ccagtagttt
     1981 ggctcagctt cttgctcatt gtgtttgaag acttgcttaa acaacccaca atgatagttt
     2041 aaaccaaccc cttcaccatt aataccaaga gatgaaatag agtcaatgaa acatgatgcc
     2101 aaacgtccta aaccaccatt ccctagtgat ggttctaatt caacatcttc gacttctgcg
     2161 atagatttgc cagcagctgc caattcttct ttgatatctt tgtaaattcc taggttaatc
     2221 aggttgtttg atagtaattt accaatcaaa aactctgctg agatatagta aactttgcgt
     2281 ttggcagtat ttttagcttt gtggctagct tcttctttga caaagtttaa taatgataga
     2341 taaatctctt cattgctggc ttgcgtaagt gattttccta gtttagtttc aacatattct
     2401 gtaaagcgtg tcatgtcttc ctcttttcaa taatgtaaca tttaaatatt atcttgcgcg
     2461 accataaaga tctgttaaat aaataaggta gtctttgtga tcttggttca attcaccgtc
     2521 aagcatacgc cattgccaat tacctccaat tgtatttggc atattcatac gactcttacc
     2581 atctttatct aataaatctt ggatgcaaag aatggctgta tcacaaacag aagcaaagat
     2641 tgttcttagc atggcttttg taatactttc atctgcatgt ttgtgaagat agttatttac
     2701 aaagtgtact tgttcttcac taagattgtc ataccatccg ttaaccacct cgttatcatg
     2761 agtccctgtg taagcgacac aattacggtc atagtaatgt ggcaaatcta tactttgact
     2821 cgttatatca aataaaccaa attctaatat cttcatgcca ggaaagcctg ttgatgctaa
     2881 tagctgctct gctttttcat caatataacc aaggttttct gcgatgatag gaagctctcc
     2941 taaggcctca cgaaccgcag aaaataacgc tatacctggg gctgaggccc aatggccatt
     3001 tctagcagtt ttatcaccag caggaatttc ccaaaaatct gagaagcctt taaaatgatc
     3061 gattcttagc tgatcatata gtttgaaact ttcttgaata cgataaatcc accatgcaaa
     3121 attagacttt tcatgagcag accaattgta ggttggattt ccccacagct gaccatcttc
     3181 actaaagcca tctgctggta cacctgcaat aaacaatggc tttttatcac tatctacttt
     3241 aaatagctca ggcatcgtcc aaacttctac actgtccgca gaaacataaa taggcatatc
     3301 accaataatt tctatccctt tatggttagc gtaagttttc aaggctgacc attgttggta
     3361 aaagaagtat tgacagactt tatgataggt aatctttttt gcaagcaact cacggtaatt
     3421 attaagtgag tcttcttggc gtttaatgac agtttcgtcg tcccaatctt gtaaggcttt
     3481 attgttaaaa tattctttta aagccataaa ttcagcaaaa tcagttaacc agctagaagc
     3541 tgtttcaaaa gcttctaatt tacacacatt ttcttgttca gcaacaaaag ctctcactgc
     3601 tttttctaaa agtggacgcc taacttgaaa aagctgagca tagtctactg cttcaggatt
     3661 tgtgccgaat gtaatatcac acaaatctgc tgcttctaaa agttcatcat ctactagcaa
     3721 ttcaaaatca atgaaatgtg tgttcccagc aatagctgaa aatgactgat aaggagaatc
     3781 tccaaaactc gtcgttgtta aaggcagaat ttgccaatag gtttgtttcg tctctgctag
     3841 aaaatcaaca aattcaaaag ctgattttcc aaaagtgcct atcccaaatt tcccaggcag
     3901 ggaactaata tgtattaaga taccgcttgc acgtttgttc atgatttttc tccttgtgaa
     3961 atctattcat gatgagatta taacgcaagc gcttgcgtaa gtcaaccagt ttcctaaaaa
     4021 aagtaacatt ccttaaaaat gctacttttt ttgttcttaa tgactaatat cacggacgct
     4081 ttctctttgt tttaaactaa aaggaacaaa aatagtctga ctaaga
//
LOCUS       DAUELZ010000111         4008 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.112, whole genome shotgun sequence.
ACCESSION   DAUELZ010000111 DAUELZ010000000
VERSION     DAUELZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.112"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            108..2594
                     /gene="gyrA"
                     /locus_tag="VHI09_001506"
     CDS             108..2594
                     /gene="gyrA"
                     /locus_tag="VHI09_001506"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEQ6121362.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDILAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELVSTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIRDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIAEINTDRENSISKNKDN"
     gene            2614..3363
                     /locus_tag="VHI09_001507"
     CDS             2614..3363
                     /locus_tag="VHI09_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEQ6121363.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIAVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQVST"
     gene            3446..3859
                     /locus_tag="VHI09_001508"
     CDS             3446..3859
                     /locus_tag="VHI09_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ6121364.1"
                     /translation="MKLNAIHHVAIIVSDYNLSKNFYVNKLGFEIIRENYRPDRHDYK
                     LDLSCGRIELEIFGKVTSDPYYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KNLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
BASE COUNT         1306 a          665 c          844 g         1193 t
ORIGIN      
        1 cagaaaaata atgaaaatag tttactgata acgcttccaa taagaaaaaa taaggaattt
       61 atggtataat gaaacctagt attgactaga aaaggagcat tccttaaatg caagatcgaa
      121 atttaattga cgtcaatctg acaagtgaaa tgaaaacgag tttcattgac tatgccatga
      181 gtgtcattgt ggcaagagct ttgccagatg tgcgtgatgg tctgaaacct gtgcatcgtc
      241 gtattctcta tgggatgaat gaactcggtg tgacacctga taaaccgcat aaaaaatctg
      301 ctcgtattac gggtgatgtc atgggtaaat atcacccgca tggggattct tctatttatg
      361 aagctatggt tcgtatggct cagtggtgga gttatcgcca tatgcttgtt gatgggcatg
      421 gcaattttgg ttctatggat ggtgatggtg ctgccgctca acgttatact gaagcgcgca
      481 tgagtaaaat tgcgcttgaa ttattaaggg atatcaataa aaataccgtt aattttcaag
      541 ataactacga tggaagcgaa agagaaccag tagtattacc agctcgcttt cctaatttat
      601 tagtcaatgg agcgacaggg attgccgttg ggatggcaac taacattccg cctcataatt
      661 tggcagagtc tattgatgct gtcaagatgg taatggaaca tccagactgt acaacaagag
      721 agttgatgga agtgatccct ggacctgact ttccgactgg tgcccttgtt atggggcgct
      781 caggtattca tagggcttat gatactggta aaggttctat cgttttacgc tctcgaacag
      841 aaattgaaac aactcaaaca ggtcgggaac gtattgtggt tactgaattt ccctatggcg
      901 tcaataaaac gaaagtgcat gaacacattg ttcgtttagc acaagaaaaa agactagaag
      961 gtattacagc agttcgtgac gaatctagcc gtgaaggagt gcgttttgtt atcgagattc
     1021 gtcgagaggc atccgcgaca gttatcttaa ataacctctt taaattaaca agccttcaga
     1081 cgaatttcag ttttaatatg ttggctattg aaaatggtgt gcctaaaata ttgtcactca
     1141 gacaaatcat tgataattac attagtcatc aaaaagaagt tattatacgt cgcacccgat
     1201 ttgataaaga taaagctgaa gccagagctc atattttaga aggcttactg attgcccttg
     1261 atcatttaga cgaagtgatt gctattattc gtaacagcga gacagatgtc attgctcaga
     1321 cagaattgat gtcacgtttc gacttgtctg aacgccaaag ccaagctatt ttggatatgc
     1381 gtttgcgtcg tttgactggt ttggaacgtg ataagattca atcagaatat gatgatttgt
     1441 tggccttgat tgctgactta tctgatattt tagccaaacc agaacgtatt atcactatca
     1501 tcaaagaaga aatggatgag atcaagcgta aatacgctaa cccacgccgt acagaattaa
     1561 tggtcggtga agtcctttct ctagaagatg aagacctaat tgaagaagaa gatgttttga
     1621 ttaccttgtc aaataaaggt tatatcaagc gtcttgctca agatgaattt cgtgcccaga
     1681 agcgtggtgg acgtggcgtc caaggaactg gggtcaacaa tgacgacttt gtccgtgaat
     1741 tggtatcaac aagtacccat gatactttgc tcttctttac gaattttggg cgtgtctatc
     1801 ggttgaaagc ttacgaaatt ccagaatatg gtcgtacagc aaaaggtctt ccaattgtta
     1861 atctattgaa attagaagat ggtgaaacca ttcaaactat tatcaacgca cgtaaggaag
     1921 aaacagcagg taaatccttc ttctttacaa cgaaacaagg cattgtcaaa cgtacagaag
     1981 tttcagaatt taataatatc cgtcaaaacg gtcttcgtgc tcttaagttg aaagagggag
     2041 atcagctgat taacgtcctt ttgacaagcg gtcaggatga cattatcatt ggaactcatt
     2101 ccggatattc tgttcgtttt aatgaagcaa gcatccgcaa catgggccga tcagctacgg
     2161 gtgttcgcgg ggttaagctt cgtgaagatg atcgcgttgt tggcgcgtca cgtattcgag
     2221 ataaccaaga agttcttgta attaccgaaa atggctttgg taagagaaca agtgctacag
     2281 actatcctac aaaaggtagg gggggaaaag gaataaaaac agctaatatt acccctaaaa
     2341 atggtcaatt agctggattg gttaccgtag atggtacaga agatatcatg gtgattacaa
     2401 ataaaggtgt cattattcgc acaaatgttg caaatatttc gcaaacaggt cgtgctactt
     2461 tgggtgttaa aattatgaaa ctagatgctg atgctaagat tgtaacattt accttggttc
     2521 agcctgaaga cagtagtatt gctgaaatca atactgatcg tgaaaatagt atttcaaaaa
     2581 ataaagataa ttaattataa caaggagtaa aaaatggtaa aaaaacaaaa gcgtcgaaaa
     2641 ataaaatcca tgtcttgggc aagaaaacta ctaatagctg tacttttaat tctaggttta
     2701 gcattgcttt ttaacaaacc tatccgaaat acattaattg ctcgaaactc taataaatat
     2761 caagtaacga aagtttcgaa aaaacaaatc aaaaagaata aagaggctaa gtcaacattt
     2821 gatttccagg ccgtagagcc agttagtaca gagagtgtct tgcaagcaca aatggcggct
     2881 cagcaacttc ctgttatagg gggcattgcc ataccagagc ttggcattaa tttaccaatt
     2941 tttaaaggtt taggaaatac tgagcttatt tatggcgcag gaacgatgaa agaagaacaa
     3001 gttatgggag gagaaaataa ttattctctt gccagtcatc atatttttgg aattacaggt
     3061 tcatctcaaa tgctcttttc gccgcttgaa agagcacaaa atgggatgtc catctattta
     3121 acagataaag aaaaaattta cgaatacatc ataaaagatg ttttcacggt agctcctgaa
     3181 cgcgttgatg ttatcgatga tacagctggt ctcaaagaag tgactttagt gacttgtaca
     3241 gatatcgaag caacagaacg tattattgtc aaaggagaac taaaaacaga atacgatttt
     3301 gataaagcgc ccgccgatgt attgaaagct tttaatcatt cttataacca agtatctacc
     3361 tagatatatc ttaaatataa tatttaaaaa gtaggaccat agagaaaatt tatctcatcc
     3421 tacttttttt gaaaggagaa atattatgaa actaaatgcc attcatcacg ttgctattat
     3481 tgtatctgat tataatctgt caaaaaattt ttatgtgaat aaactcggat ttgaaattat
     3541 cagagaaaat tatcgtcctg acaggcatga ttacaaatta gatttatctt gtggtcgtat
     3601 tgaattggag atttttggta aggtgacaag tgacccctac tatcaagctc ctcctaaacg
     3661 tgtaagtcaa ccagaattta aaagtgaggc ctgtggtttg aggcaccttg cttttcgtgt
     3721 taccaacatt gagagttatg tagatgatct aaagaactta ggaattccag tggagcctat
     3781 caggcatgat gattacacag gtgagaaaat gactttcttt tttgatccgg atggtttgcc
     3841 tttagaatta catgaatagt cttattaggt taacgactgg atcgtgctat taaactcgtt
     3901 aaaaatagtt atttgttgca tcttcttttg cttgattaat ctcttttaat cgttaaatga
     3961 tttggttaac tttcaaagtg aagagcaatt tttggagtaa tcttgtcg
//
LOCUS       DAUELZ010000112         3996 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.113, whole genome shotgun sequence.
ACCESSION   DAUELZ010000112 DAUELZ010000000
VERSION     DAUELZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.113"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            101..1099
                     /gene="ruvB"
                     /locus_tag="VHI09_001509"
     CDS             101..1099
                     /gene="ruvB"
                     /locus_tag="VHI09_001509"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEQ6121365.1"
                     /translation="MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            1237..1674
                     /locus_tag="VHI09_001510"
     CDS             1237..1674
                     /locus_tag="VHI09_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEQ6121366.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     KPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMNKEY"
     gene            1698..2099
                     /locus_tag="VHI09_001511"
     CDS             1698..2099
                     /locus_tag="VHI09_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121367.1"
                     /translation="MIDMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            2096..3871
                     /locus_tag="VHI09_001512"
     CDS             2096..3871
                     /locus_tag="VHI09_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEQ6121368.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLLWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNFSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMVGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGALALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPKGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
BASE COUNT         1221 a          625 c          832 g         1318 t
ORIGIN      
        1 ctatctttta aagtagttca agagcttagt gctgttgact ggtggcgatt ataataaaag
       61 taggacactg ctctctttta ctttatgtta taatagtcct atggctagaa ttttagacaa
      121 taatgtaatg ggaaatgagg agttttcgga tcggactctt cgccctcaat atctacacga
      181 atatattgga caagataaag ttaaggaaca attcgcaatt tttatagaag cagctaaaag
      241 acgtgacgaa tctttagacc atgttttatt atttggacct ccaggactgg gaaaaacaac
      301 aatggcattt gtgattgcca atgaattggg agttaattta aagcaaactt caggtcctgc
      361 cgttgagaaa gctggagatt tggtagctat tttaaatgaa ttagaaccag gggatatttt
      421 gtttattgat gaaattcacc gcatgccaat gtctgtcgag gaagtactat atagtgctat
      481 ggaagacttc tatattgata ttatgattgg cgctggagat actagtcgta gtattcattt
      541 ggatctgcct ccatttacat tgattggtgc tactacacgt gcaggcatgt tatctaatcc
      601 cctcagagct cgttttggta tcactggtca catggaatac tatcaagaaa aggatttgac
      661 agagattgtg gagcgtacag ctactatttt tgaaataaaa atagaccatg aagctgcacg
      721 gaaattagct tgtcgcagta gaggaacgcc ccgcattgct aatcgattac taaaacgagt
      781 gcgtgactat gctcaaatta ttggtgatgg catcataacg gctcaaataa ctgatagagc
      841 tttgactatg ttagatgttg accgcgaagg tctcgattac attgatcaga aaattttacg
      901 aacgatgatt gagatgtatc agggaggacc agttggtttg gggacattat ctgtgaatat
      961 tgccgaggag cgtaatacag ttgaagagat gtatgaaccc tacttaattc aaaaaggctt
     1021 tctcatgcga acacgaacag gtcgtgtagc gacgcaaaag gcttatcgtc atcttggcta
     1081 tccctatcaa aacacttaat gatttttcaa atctaagaaa attaatctga acctgctttt
     1141 tgtgttcgga ttttttgata agttgacaga aaattattag attgatttct ccgatatcag
     1201 ttagaaattt gatataataa ttcaaaggag tatttaatga aaaaagtatg ttttgtctgt
     1261 ctgggtaata tttgtcgaag ccccatggct gagtttgtaa tgaagtcaat agtatcaagt
     1321 gatgttatga tgatagaaag tcgtgctact tctgattggg agcatggaaa acctattcac
     1381 tcaggtacac agtccatatt aaaaacttac caaattaatt atgatataac taaatgctcc
     1441 aagcagataa ctataactga ttttaacacc tttgactata ttattggtat ggatagcgat
     1501 aatgtgaaaa atctcaaaga gatgtcgcaa catcaatggg actctaaaat ctatctgttt
     1561 agagaagggg gtgttcctga tccttggtac actaacgatt ttgaagaaac ctaccaatta
     1621 gttagaaaag gatgccagga ttggctttct cgtttaatga ataaagagta ttgataatta
     1681 gaagttgagt aaaagaaatg atagatatgc aagatatagt aaaaaaatgg agtattacac
     1741 gggcgaagtt ggaaattgtc tcggtgattg tcatattggt ttgtgcaatt tctgttttta
     1801 gcgtaagaat ttcgaataag acaagtttga cctatgataa agggcgtatg cactatactg
     1861 gttacgtgat taaccataaa atgaatggag aagggaagct tgtctacccc aacggtgata
     1921 tctatgaggg tacttttaag aatggcctat ttgaaggaaa aggaactttt actgctaaga
     1981 cgggctggtt atataacggg gagtttcata aaggacaagc aaatggcaaa ggtgtgttaa
     2041 aagcaaagaa taataaagtc tataaaggga tttttaaaca ggggatattt caaaaatgag
     2101 aataaaatgg ttttcgtttg ttagggtaac aggactttta ctggttttac tctaccactt
     2161 ttttaaaaat gtatttccag gaggttttat tggtgttgac attttcttca ccttttcagg
     2221 atatctaatc acagccctct tgattgatga atacacaaaa aaagagtcta tcgatattat
     2281 cggtttttta aaaagacgct tttatcgaat tgttcctcct ttggtgttaa tgattttatt
     2341 gaccattcct tttacgtttt taattaagaa ggatttcatt gctaatatag gaagtcagat
     2401 tacagctgtt ttaggattta ccactaatat ttatgaaata ttgacgggaa gtagttatga
     2461 aagccaattt attccacatc tctttgttca tacgtggagc ttagctattg aagttcattt
     2521 ttacttgttg tggggtgtat ttgtttggtt attagctaga cgcaaggaga ctcagaaaca
     2581 actgagagga ctactgtttt tgatttcttt gggaatattt gctattagtt tcttgagcat
     2641 gtttataaga tcttttatga cctcaaattt ttccctgatt tatttttcga gtctttctca
     2701 cagttttccc ttctttttag gggcgatgtt tgccacgata acaggtatta acgagactac
     2761 tgttcgattt caaaaaaatg tacgtttatg gccgcgccag tatgtccttg ctgctatggt
     2821 tggagcattt acacttttgc ttgtgctaac ggtcacattg gattttaacc atattacgac
     2881 ctatctattt ggctttgctt tggctagctt attcgcatca attatgattt atgcagcgcg
     2941 tgttcttcat gagcaaacac cagatgtaca agagcctaaa gctatcactt atatagctga
     3001 tattagctat gggatttacc tcttccattg gccattttac attattttta gccaattaat
     3061 gtctcatatt ttggcagtta ttctaacagt tttcttttca atcttgtttg ccactgtgtc
     3121 ttactatatt gtggaacctt tggtccaagg gagaaaacca aacctgcttg gtttagaaat
     3181 tgactgttct ccttactata agtggattgt aggaggggct ctcgccttag cattattaac
     3241 tttaggaact tgtatgatag cgcctaaagt tggtaagttt gagaaacaat tgttggtcag
     3301 ttccctacaa caggctcaaa gtaatatgga gcggacacat acactagctg caggagacgc
     3361 taatgctttg agtgacgtag gtattattgg tgattctgta gcattacgct ctagtgcggc
     3421 attctcaaaa attatgccac aagcgcaact ggatgcagcg gtcagtcgga attttaaaga
     3481 agctttcgat ttatttaata accaaattaa gtctaagagt ctatctaaga ctgttgtctt
     3541 agcagtaggt gttaactcgc ttgacaacta ttctcaagca gttcagtctt ttattgaggc
     3601 attgccaaaa ggtcatcgtt tagttttggt ttctccatat aatgccaaaa atgccagtca
     3661 ggttgcagaa gctagagact atggtcttaa attgtccaaa aaatacaagt atgtcactat
     3721 tgcagactgg tataaagttg ctgttgaaca tccagatatt tggtatggca gtgacggtgt
     3781 tcattatagt gaagatagtc aaggagcaga actatatgtc tccacaatcc agactgctgt
     3841 tgagaagtca gctaaaaaac cagctaaata aagttaagaa atgtctaaga gataagtcag
     3901 atccattgtg ctctggcttt ttcttgtgaa caaatgatga tggtaaaata gtagtacttt
     3961 gaggttttta aaagaaagcg gtttcagaaa taaaac
//
LOCUS       DAUELZ010000113         3977 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.114, whole genome shotgun sequence.
ACCESSION   DAUELZ010000113 DAUELZ010000000
VERSION     DAUELZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.114"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..2449
                     /gene="ppc"
                     /locus_tag="VHI09_001513"
     CDS             <1..2449
                     /gene="ppc"
                     /locus_tag="VHI09_001513"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEQ6121369.1"
                     /translation="LGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTILE
                     LTTHIHKLLRKYRDAKTGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVMQ
                     YYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCLS
                     ATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRRA
                     FHYIQSRLKQTQIQLTNQPAARMSSSVGLNTSAWSSPASLENPILAYDSPVDFKADLK
                     AIEQSLLDNGNFALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQEACVAELLKGANI
                     VDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGEE
                     VIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAAY
                     LSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKIT
                     FFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEML
                     ISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQG
                     NLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEWQ
                     LTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDEK
                     LIHTTINGIATGLRNSG"
     gene            2589..3893
                     /locus_tag="VHI09_001514"
     CDS             2589..3893
                     /locus_tag="VHI09_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEQ6121370.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILV
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
BASE COUNT         1173 a          697 c          832 g         1275 t
ORIGIN      
        1 tttagggaaa ttagccctaa ccatcaagga tcttgctgga aaagataatg gtaaagacat
       61 tttggagcag gttaatgtgg ttcctgtttt aacagcacat ccgacgcaag tacaacgtaa
      121 aacgattctg gaattgacaa ctcacattca taaacttctg cgcaaatacc gtgatgctaa
      181 gactggtgtg attaatcttg agaaatggcg tcaggagcta tatcgttata ttgaaatgat
      241 catgcagaca gatattattc gcgagaaaaa gcttcaggtg aaaaacgaga taaaaaatgt
      301 catgcagtat tacgatggtt ctctcattca agctgtgacc aagttgacaa cagagtacaa
      361 aaacttagct caaaaacacg gtctagagtt agacaatccc aaacccatta ccatggggat
      421 gtggattggt ggggaccgtg atgggaaccc ctttgtcaca gctgaaacct tgtgcttgtc
      481 agctacggtt caaagtgagg ttatcctcaa ttactacatt gacaaattag cagcccttta
      541 ccgaacattt tcactgtcgt caaccttggt acaacccaac tcggaagtgg aacgtttagc
      601 cagtttgtct caggaccaat cgatttaccg tggaaatgaa ccttatcgca gagcttttca
      661 ttatattcaa tcgcgtctta aacaaacaca aattcaacta actaatcagc cagcagctcg
      721 tatgagtagt agtgtgggac taaacacatc tgcttggtcc tcaccagcta gtttagaaaa
      781 tcccatctta gcctatgact ctcctgttga ttttaaagct gatttaaagg ctattgagca
      841 gtcgctactt gataatggca acttcgcttt gattgagggg gatttgcgag aagtcatgca
      901 agctgttgat atttttggct tcttcttagc aagcattgac atgaggcaag actccagtgt
      961 ccaagaagct tgtgtggcag agttattaaa aggagccaat attgttgacg attatagctc
     1021 tctttcagaa actgaaaaat gcgatgttct cttacaacaa ttaatggagg agccaaggac
     1081 tttatcctca gcggcagttg ctaaatcaga cctattagaa aaagaactgg ctatctatac
     1141 tacggcacga gaattgaaag ataagcttgg agaagaggtt ataaagcaac atattatttc
     1201 acatacagaa agtgtctctg atatgtttga gttggccatt atgcttaaag aagtgggatt
     1261 ggttgaccag cagcgggctc gtgtgcaaat tgttcccctc tttgaaacca ttgaggattt
     1321 agataatgcc cgtgacatca tggcagccta tttaagccat gacattgtca aatcttggat
     1381 tgcaacgaat cgtaattacc aagaaatcat gttaggctat tctgacagta acaaagatgg
     1441 tggctacttg gcatcgggtt ggactcttta taaagctcag aatgaattaa cggctattgg
     1501 tgaggaacat ggagtgaaaa tcaccttctt ccatggtcgt ggaggaacag ttggtcgagg
     1561 aggtggccct tcttatgatg ccattacttc acaacctttt ggttccatca aagatcggat
     1621 tcgtctgaca gagcaagggg aaattattga aaacaaatac ggcaataaag atgtggctta
     1681 ttaccattta gaaatgttga tttcagcttc tatcaatcgt atggtgactc aaatgataac
     1741 agatcctaat gagattgata gttttcgaga aatcatggat agcattgttg ctgatagcaa
     1801 taccatttat cgaaaattgg tttttgacaa tccccatttc tatgattatt tctttgaggc
     1861 aagcccgatt aaggaagttt ccagtctcaa cattgggtca agaccagcag cccgtaaaac
     1921 gattacggaa attacaggct tgcgtgccat tccttgggtc ttttcatggt cacaaaatcg
     1981 catcatgttc ccaggttggt atggggtagg ttcagccttt aaacgttata ttgaccgagc
     2041 acaaggtaac cttgaacgcc tccaacacat gtatcagacg tggccttttt tccattcctt
     2101 gttatcaaat gttgatatgg tattatctaa atcaaacatg aatattgcct ttcagtatgc
     2161 acaactagct gaaagtcagg atgttcggga tgttttttat gaaattttgg atgagtggca
     2221 attgacaaaa aatgttattc ttgctatcca agaccatgat gatctattgg aggataatcc
     2281 ctcccttaag catagcttga agtcacgctt gccgtatttt aatgtgttga actatattca
     2341 aattgaattg attaaacgtt ggcgtaataa tcaacttgac gaaaatgatg aaaaattgat
     2401 ccatacaact atcaatggca tagcgactgg tttacgtaat tcaggctaat gctcttgagt
     2461 attaattggt atttatagaa gttttcaagt gactaatcag aattttaaag gttagccctt
     2521 ggagcttctt tttttgagga ttcttgactt tttcttttgt ttgatataga aagatgttat
     2581 aatgctaaat gattatctca agaagtagag ggaaaactat gaagattgat aaaaggcacc
     2641 tcttaaatta ttctatatta ttgccttatt taattttatc ggtgataggc ttaataatgg
     2701 tttactcaac cacaagtgtc agcttgattc aagcacatgc taatccattt aaatcggtga
     2761 ttaaccaggg agttttttgg ataataagtc tagtggctat tacatttatc tataaattaa
     2821 aattaaattt tttaacgaat acaagggtat taactgtggt tatgttagga gaggcctttc
     2881 ttttaattat tgcccgcttc tttacaactg ctatcaaagg agcgcatggt tggattgtga
     2941 tcggaccagt cagctttcag cctgctgagt atttaaagat cattatggta tggtatttgg
     3001 cattgacctt tgctaagatc caaaaaaaca ttagccttta tgattatcaa gcactaactc
     3061 gtcgaaaatg gtggccaaca caatggaacg atttacggga ctggcgtgtt tattctttgt
     3121 tgatggtact tttggttgct gctcagcctg acttgggaaa tgcttccatt attgtattga
     3181 cagccattat tatgttttct atcagtggca ttggttaccg atggttttcg gctatcctag
     3241 tcatgattac cggtctatcc acggtctttc ttgggactat tgccgttatt ggggtagaaa
     3301 gagttgctaa gattcctgtt tttggctatg tcgctaaacg ttttagtgcc ttttttaatc
     3361 ctttccatga cttgaccgat tcaggtcacc aattagccaa ttcttactat gctatgagta
     3421 atggtgggtg gtttggccaa gggttaggaa attccattga aaagcgtggt tatttgcccg
     3481 aagcacagac agactttgtc ttttcagttg tcattgaaga gttaggtcta ataggagcag
     3541 ggtttatttt ggcacttgtt ttcttcttaa ttttacgtat tatgaatgtt ggtattaaag
     3601 caaaaaatcc ttttaatgcc atgatggctt taggagtagg cggtatgatg ctcatgcaag
     3661 tctttgttaa tattggtggt atctcagggt taattccatc tactggtgtt accttccctt
     3721 ttttgtctca aggtggaaat agcttacttg tcttatctgt tgcagtgggt tttgtactaa
     3781 atattgatgc tagcgaaaag cgagatgata tatttaaaga agctgaatta tcttatcgaa
     3841 aggacactcg aaaagaaaat agtaaagttg taaatatcaa gcaatttcaa taatattcta
     3901 aaatctcctt taaggagatt ttagaatata gacaatccca gtttgatgct caaaaaactt
     3961 gaactgctct gctgatt
//
LOCUS       DAUELZ010000114         3844 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.115, whole genome shotgun sequence.
ACCESSION   DAUELZ010000114 DAUELZ010000000
VERSION     DAUELZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.115"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..1057)
                     /locus_tag="VHI09_001515"
     CDS             complement(<1..1057)
                     /locus_tag="VHI09_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ6121371.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKIFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQTSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFVKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNDI
                     KMGRLSEQLIKKTRSMLL"
     gene            1367..1780
                     /gene="yaaA"
                     /locus_tag="VHI09_001516"
     CDS             1367..1780
                     /gene="yaaA"
                     /locus_tag="VHI09_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEQ6121372.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRIGDKISLPDQDLIIIIVEPNQEEKEQFAEEMAEKTRVAALVKQMNQANKK
                     TSSKHNNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
     gene            1782..2888
                     /gene="recF"
                     /locus_tag="VHI09_001517"
     CDS             1782..2888
                     /gene="recF"
                     /locus_tag="VHI09_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEQ6121373.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLFELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTSIYQQFLHQLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQVDSDIH"
     gene            complement(2944..3807)
                     /locus_tag="VHI09_001518"
     CDS             complement(2944..3807)
                     /locus_tag="VHI09_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEQ6121374.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIVGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLDETKTKKEMRWVVTGIICFI
                     IGAILLGVVKS"
BASE COUNT         1329 a          712 c          617 g         1186 t
ORIGIN      
        1 tcaacaacat cgacctggtc tttttaatca attgttctga taatcgtccc attttaatgt
       61 catttaattc ttggatgatt agttgtaacg tttttgtgcg atgttgactg tcaattccag
      121 tataaatttc aaataaacct gtataactat caaaacgaca ccctatacta taagcaagtc
      181 cttcttcttc tctaattttt acaaagagac gggaatgtgc aaaagagcct aacaacccat
      241 ttaaaagaac taaagcataa tagtcacgtt gtccaaaaac tgaagggaag tgataagcta
      301 attgtaaaat agattgatga actgctcttt tttcaataga ttctttaata atattgacag
      361 aagtttgtaa atgaaagaaa tttaggtttt tatttcggtt atcaagtgga aattgatgaa
      421 ttagctgaac aacgcgataa tcatcaaaat ctcctaaaat gaaaatatca atctgatctt
      481 cattgagcat tttatgaaat tcttgatagc tagtataggc agtctctttt gcaattagtt
      541 ctgggctacc atattctgac atttgtagat tcttattaca ataaaacaat tcttttacct
      601 taagagagct gtagtaaaaa gaatcctctc tatctgactc gatgtagtta atcaaattat
      661 ttttttcagt ctcaaaaatt ttaggctgat actgggcgat ggataatagg ggtgagaaca
      721 agatatcttt taaaaactga atcatttcat ctagtatttt ttccccattg caagcatatc
      781 tatcttgaat aaaagtaata tcgatatcca caatatgaac taaccctttt gttaacacgt
      841 tagttgacaa gctagctcca taaagtcgtg ctaatttttc tctaaactgc ctaacagttg
      901 gataacactc attagctgtt gctaacatct gtgccactaa gactcttttt gctactgttt
      961 tttggttcaa atcacctgaa aaacgaaagg taatatgatt ggttttaaac tgtttggtct
     1021 taatcagatg aagttgtact ccctgaacaa ttttcataat gtttccttaa taaatagtgt
     1081 gatccttacg attataccat ttttattcag gttaggtgag tgataacttt ttaaattcct
     1141 tacggttaaa caaaaaaaga agcaattgac ttgctcctat gatttactgc aaaataaagc
     1201 cagtaatacc tctaaaaagt ttcaaccaca ctttatactt aaaaatacag attatattat
     1261 atcaaataac gtctcacttt ggaatagtca aatagctctt attcagctat tattatttat
     1321 ttctgtttta tgctataatg acttgaatga caaaatggag atgacaatga tatacaaatt
     1381 atttacagaa tttatcaccc tacaagccct tcttaaagaa ttaggaatta tccaaagtgg
     1441 tggcgctatc aagggatttc tcgctgaaac aaccgttcta tttaatggtg aagatgagaa
     1501 acgtcgaggc aaaaaaatca gaatagggga taagatttct ctacctgacc aagacttaat
     1561 tatcattatt gttgagccta atcaagaaga aaaagaacaa tttgccgaag aaatggctga
     1621 aaagacacgt gtagcggctc tagtgaaaca aatgaaccaa gctaacaaaa agacttcttc
     1681 taaacataac aatcgtcaat ctactaccaa aaaatcacta agagcaacta agaaaactaa
     1741 aggtaaacca actgcacctg tccgcttccc aggtatctag tatgtggatt aaagagttag
     1801 agttaaaaca ttatcgcaat tatgaccacc tgcttgcctc attttcgtcg ggtttaaatg
     1861 tctttatcgg taataatgct caaggaaaaa ccaattttct cgaggcaatc tattttctat
     1921 cacttactag aagtcatcgc acgagggctg ataaagaatt gattcatttt gatcactcaa
     1981 ctgtttctct tactggtaag attcagcgta ttagtggtac tgttgattta gaaatcaatc
     2041 tatcagataa aggacgtgtg acaaagatta atgcgctaaa gcaagcaaaa ttatctgatt
     2101 atattggaac gatgatggtt gtgcttttcg caccagaaga tttgcaacta gttaagggag
     2161 cacctagcct tcgtcgcaaa tttatcgaca ttgaccttgg tcaaattaag cctgtttact
     2221 tatttgagtt atcccattat aaccacgtgc ttaaacagcg taatagctat ttaaaaagtg
     2281 cccaacaaat tgatgccgct tttctagctg ttctagatga gcaattagct agctatggag
     2341 cccgtgtcat ggaacatcgt attgatttta tcaatgcgtt agagaaagaa gcaaatactc
     2401 atcaccaagc catctcaaat ggtttagaaa gtctctctct ctcctatcag tcgtcagttg
     2461 tctttgacaa aaaaactagt atttaccaac aatttttaca ccaacttgaa aaaaatcatc
     2521 agaaagattt cttccgtaaa aacactagtg ttggtccaca tcgcgatgac ttagcctttt
     2581 acattaacgg gatgaatgct aactttgcta gtcaaggtca gcaccgcagc cttattcttt
     2641 ccttaaaaat ggctgaagtt agtttaatga aggccttgac tggagataat cccattcttt
     2701 tactagacga tgtcatgagc gagctagaca atacacgaca aacaaagtta cttgaaacag
     2761 taattaaaga aaatgtccaa acctttatca caacaacaag tttggatcac ctgtcacagt
     2821 tgccagaagg tatccgtatt tttcacgtga caaaaggaac cgttcaagtt gattctgaca
     2881 ttcactaata acatcaaaaa agggttcagt taaactgagc ctttttttga tgttatgtgg
     2941 cgattaagat ttgaccacac ctaataaaat cgcacctatg ataaaacaga taatacctgt
     3001 gacaacccaa cgcatttctt ttttggtttt ggtttcgtct aggaataaaa tgcctccgac
     3061 aatcgaaatg atagcaccta attgtgagaa actaaaggca atagcaagtc ctgcttttga
     3121 tgcagctaaa agcataaaaa tattcccaat gccccaaagt aatccaacaa cgctattttt
     3181 aatcacatac tggtcaatgg aaattttaaa tgacatgaaa gttatagctc ctaaaaccat
     3241 acctactgcc atgggtaaaa tgactgacaa aacttcaaat ttcatgatgt tattaaataa
     3301 tacagcatac atcacgtaac caattgttga ataagtgagc gccctaaatc cttttgaaaa
     3361 attatgaagg tgattgactt gtgcattagc atcatcttgt ttactagaaa agtaaaaacc
     3421 aacaatcaga agtaatagtg ctagacttcc tactacaaat tgcataggtc ttgtccattc
     3481 atgaaaaacg agcacaccaa tcaaacttcc cagaacaagt tgagagccac ttgacaaagg
     3541 attggcaacg gagactccca tgtattgcat ggcatgaaat tgaccagttt gaccaattga
     3601 ccagataaaa ccacccaata ttccaaaaag ccaaagttgc agtgtcatct caggtctaac
     3661 aattaaccaa acagctaatg aaaataataa agccccaaaa gtcattccta gagtttgttg
     3721 cgaaggtttt cctccaattt tgttactaac aaatccaatg cttccccatg taaacatggg
     3781 aataagtgca taaaaaatac cttccaaaat gttatctcct ttatagtaaa cctttaaaga
     3841 ttat
//
LOCUS       DAUELZ010000115         3843 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.116, whole genome shotgun sequence.
ACCESSION   DAUELZ010000115 DAUELZ010000000
VERSION     DAUELZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.116"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            9..506
                     /locus_tag="VHI09_001519"
     CDS             9..506
                     /locus_tag="VHI09_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEQ6121375.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAIL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPHLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            526..696
                     /locus_tag="VHI09_001520"
     CDS             526..696
                     /locus_tag="VHI09_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121376.1"
                     /translation="MQRSIFGVFTAFLCVICVLCAIPAFKKNRYGLASLFLLNAFTNL
                     VNSIHAFYGTLF"
     gene            826..2055
                     /gene="clpX"
                     /locus_tag="VHI09_001521"
     CDS             826..2055
                     /gene="clpX"
                     /locus_tag="VHI09_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEQ6121377.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIQILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            2065..2664
                     /locus_tag="VHI09_001522"
     CDS             2065..2664
                     /locus_tag="VHI09_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEQ6121378.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPQDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDNKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTSRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDKSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            2812..3555
                     /locus_tag="VHI09_001523"
     CDS             2812..3555
                     /locus_tag="VHI09_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121379.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKVLKKSPSSV"
     gene            complement(3613..>3843)
                     /locus_tag="VHI09_001524"
     CDS             complement(3613..>3843)
                     /locus_tag="VHI09_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ6121380.1"
                     /translation="NQTTAKKGISLTIADDAKAHLIDLGYNRAMGARPLRRIIEQEIR
                     DRITDYYLDHPEVKKLQAILKEGQLVIRQNDQ"
BASE COUNT         1254 a          620 c          784 g         1185 t
ORIGIN      
        1 aataattaat gacaaaggaa attattgcta tttgggcaga ggatgaagca gggcttattg
       61 gtgttgcggg taagttacca tggtatttac caaaagagtt agaacatttt aaaaagacaa
      121 cgctacatca agcaatctta atgggccgtg tgacttttga aggaatgaat tgcaaaagac
      181 ttcctcaacg tcaaaccctt gtcatgacaa gaaatagaga ttatcaagta gatgaggtgt
      241 tgacaatgac tagtattgaa aaagtcttgg agtggtacca tgcgcaggat aaaacacttt
      301 atatcattgg tgggaataag gttttagaag cgtttaatgg ttatttcgat cgtatcataa
      361 aaacagttat ccatcatcgt tttaaaggag atacctaccg accacatcta gattttagtc
      421 atttcacgca agaaagccaa acgttctatg ctcgagacgc taaaaatcct tatgatttta
      481 ccgttactgc tttaaaacac aaatagtctg aggagatgat aagcgatgca acgtagtatt
      541 tttggagtat ttacggcttt tttgtgcgtg atttgtgttc tttgtgccat cccagctttt
      601 aaaaagaatc gttatggctt agcaagtctg ttcttattaa atgcgtttac taatctggtt
      661 aatagtattc acgccttcta tggtacattg ttttgagatg cgacttgcaa agacagcact
      721 ggtgaagatt tagctatcaa gcaagaatca ccaaaaacca atacaacaca ttgactaata
      781 ttaaaattca gtaaaagata agagaaagta agagaattat aagaaatggc aggaagtaga
      841 actaacgata ttaaagtata ctgttcattt tgtggtaaaa gccaggatga cgttaaaaaa
      901 attatcgcag gaaataatgt tttcatctgc aatgaatgcg tagctttatc acaagaaatt
      961 ataaaagaag aattggctga agaagtcctt gccgatttga cagaagttcc aaaaccaaaa
     1021 gagctccttg atgttttaaa tcaatatgtg gttggtcaag atcgtgctaa acgtgcttta
     1081 tcagttgctg tgtacaatca ttataagcgt gtctcgttta cagaaagtcg tgatgacgat
     1141 gatgtggatt tacaaaagtc caacattttg atgattggac caacaggttc aggaaaaacc
     1201 ttcttagcac aaactctggc taaaagcctc aatgtaccat ttgctattgc agatgcaact
     1261 tctttgactg aagcagggta tgttggagaa gatgtggaaa atattttact caaactgatt
     1321 caggctgctg actataatgt cgaacgagca gagcgtggta ttatttatgt tgatgaaata
     1381 gataagattg ctaaaaaagg tgagaacgta tctatcacac gtgatgtatc tggtgaaggt
     1441 gtacagcaag cccttcttaa aattattgaa ggtacggtag caagtgttcc cccacaaggt
     1501 gggcggaagc accctaatca agaaatgatc caaattgata caaaaaatat cttgtttatt
     1561 gttggcgggg cttttgatgg catcgaggag attgttaaac aacgtttagg tgaaaaagtt
     1621 attggttttg gtcaaaacag ccgtaaaatt gatgacaatg cttcttatat gcaagaaatt
     1681 atttcagaag atattcaaaa gtttgggctt attccagaat ttattggccg cttaccagtt
     1741 gtggcagccc ttgaacaact aaacacatca gatttgattc aaattttgac agaaccaaga
     1801 aatgccctgg tcaagcaata tcaggccctt ctttcttacg atggcgtcga attggcattt
     1861 gataaggaag ccttagaagc cattgccaat aaagccattg aacgtaaaac aggagcacgt
     1921 ggtcttcgtt ctattattga agaaacgatg ttagacatta tgtttgaaat tccaagccaa
     1981 gaagatgtca caaaagtgcg tatcaccaag gctgctgtcg aaggcaagtc aaaacccgtt
     2041 ttagagacag cttaaggaga aataatggct gaagaacaag tcttaaatac ccataacgcc
     2101 tcaattttat tgagcgctgc caataaatct cattaccccc aagatgattt gccagaaatt
     2161 gcccttgctg gacgttcaaa tgttggtaaa tcaagtttta ttaataccat attggggcgc
     2221 aaaaatttgg caagaacctc aagcaagcca ggaaaaaccc aactattaaa ttttttcaat
     2281 attgataaca agcttagatt tgtggatgtc cctggttatg gctatgccaa ggtttctaaa
     2341 agtgaacggg ccaaatgggg aaagatgatc gaagaatact taaccagcag agacaatctg
     2401 cgtgcagtcg tgagtttggt tgatttacgt catgcccctt caaaagaaga tattcagatg
     2461 tatgactttt tgaagtacta tgacattcct gttattgttg tggcaaccaa agcagataag
     2521 attcctcgtg gcaaatggaa caaacatgag tctgtggtca agaaagcttt gaattttgac
     2581 aaatctgata cttttatagt attttcttcg gtagaacgta ttggtatcga tgattcttgg
     2641 gatgctattt tagagcaggt ctagcgatag gtgttgattt atctatgcta aaacattctc
     2701 agtagagggt gtttttctta tcgaaaacat aaaaaatcat agttaggttt accgatttta
     2761 cttgacgcct taatgatgac atgctagtat tttatattaa ggagatgagt catgacacaa
     2821 gtagcccaag ggcgctctaa aggaaagtca ttgttatgcc atcaatgcaa tgcccttttt
     2881 gtatttatta tttttcttat cttgcttgct gtactttttg gtgttcgata tcgtaatagc
     2941 tctattgaag gtatctggcg aacaacaagc attgatcaga agttaggcga tgattttgct
     3001 aaacgtctta ctggtttgca tcagagccct cttattgatg atagtttatt aacgtcatct
     3061 cagatgatac taacagttaa aaataacaac gttgacttga gttttagtgt tcaagttgaa
     3121 cgagacattt ttgtcaaacg tttagctgct tatcaccaaa atgaattatt aaagacatta
     3181 aaggaaaatc atttagttgt tggtgacttg agtagtaaag aacgccaaat tattgagaat
     3241 agtatgccag ctagccatga attagaaatg attttagatc aagcttttga gaaattagct
     3301 tctcagatcg gaggcaaata taatcaaaaa acaggacatt tatctgctgt tgttctcaaa
     3361 ggaaaagtta atcgtatttt gcacaccatt gatatcaaag aagaagtagc agcgggacat
     3421 acttcttttt caaaaggatt gctcacacct aatggttatt ttgattacac cagatttgga
     3481 aagaaacttg agttattagg cgatgaaaaa attattttta aaaaagtgct aaaaaaaagt
     3541 ccgtcaagtg tataacgtag tcacaagacg gattttttca tgttttgaaa attattcaat
     3601 tgaagaagtt tattactgat cgttttgcct aatcactagt tgtccttctt ttaggattgc
     3661 ctgaagtttt ttgacttctg gatggtcaag ataataatca gttatacggt cacgaatttc
     3721 ctgttcgata atacgtcgta atggacgagc tcccatagcg cgattgtacc ctaaatctat
     3781 caaatgagct ttagcgtcat cagcaatcgt taaactaata cctttttttg cggtggtttg
     3841 att
//
LOCUS       DAUELZ010000116         3808 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.117, whole genome shotgun sequence.
ACCESSION   DAUELZ010000116 DAUELZ010000000
VERSION     DAUELZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.117"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            245..2977
                     /locus_tag="VHI09_001525"
     CDS             245..2977
                     /locus_tag="VHI09_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEQ6121381.1"
                     /translation="MINKKYIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTPLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLDVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVTKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYADIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      3142..3257
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            3255..3755
                     /locus_tag="VHI09_001526"
     CDS             3255..3755
                     /locus_tag="VHI09_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEQ6121382.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
BASE COUNT         1218 a          632 c          758 g         1200 t
ORIGIN      
        1 catgtatcga atgctataaa gctgtcttgt tcgctatcag gggtgcttgt gatgctatgt
       61 cagatcaaac aagtctttaa ttgggtgact taacagatta aaagaaaaga tctaatcgtg
      121 ttacttgata gtataattaa tgcaaacaga aagtgtatcc caattcatag tctcaggtgg
      181 aggttaatat tcaaaaataa aatcttgttg ttgtcttaaa ctaatcacat taaaaggaga
      241 tatcatgatt aacaagaaat atataatacc tgtttcattg ttgacactag ctattacgct
      301 tactagtgtt gaagaagtta cttcacgtca aaatttgact tatgccaatg aaatcgtaac
      361 acaaaggcca aagagagaat ctgttattag tgataaatcg aattttcccg tcatatcacc
      421 ttacctagca agtgtggatt ttggtgagag aaaaacacct ttgccaacac ctgataaagg
      481 agtaaaagta actactgaac agtctattgc tcaagtaaga aaggggcctg aagaaagaac
      541 ctatactgtt actggcaaga ttacgagtgt gatcaatggc tggggaggct atggctttta
      601 tattcaagat agtgaaggta ttggacttta tgtttatcct caaaaagatt taggatacag
      661 taagggagat attgttcaat taacaggtac acttactcgc tttaaaggtg atttacaact
      721 ccaacaggtg actgcacaca aaaagttaga gttatctttt ccgacttctg ttaaagaagc
      781 agtaatatca gaattagaaa caacaacacc ctcaacatta gttaagttat ctcacgtgac
      841 agttggagaa ttatcaactg atcaatataa caacacatct ttccttgtaa gggatgatag
      901 tggtaaaagt atagttgttc atatagatca tcgtacaggg gttaaagggg ctgatgttgt
      961 tactaaaata agtcagggtg atttgattaa cctcacagcc atattgtcta ttgttgatgg
     1021 tcagttacaa ttaagaccgt tttctcttga acaattggat gtggttaaaa aggttacaag
     1081 ctcaaatagt gatgcttcat ctcgtaatat tgtgaaaata ggcgagattc aaggagctag
     1141 tcatacgtcg ccacttctca aaaaagcggt caccgtagaa caggttgttg tcacttattt
     1201 agacgattcc actcattttt atgttcaaga tcttaatggt gatggtgatt tagcgacttc
     1261 agatggtatt cgtgtttttg ctaaaaacgc taaggttcaa gtcggcgatg ttttgaccat
     1321 ttcaggtgaa gtggaagaat tctttggtcg tggttatgag gaacgtaagc agactgacct
     1381 taccatcacc caaattgtgg ctaaagcagt gaccaaaaca gggacagctc aagttccatc
     1441 accgcttgtt ttagggaaag atcgtatcgc gccagccaat attattgata atgatggctt
     1501 gcgtgtgttt gatccagaag aagacgctat tgattattgg gaatcaatgg aaggcatgtt
     1561 agtggcggtt gatgatgcta aaatccttgg tccaatgaaa aataaagaaa tttatgtctt
     1621 acctggctct agtacaagac cgttaaataa ttcaggtgga gtattacttc cagctaattc
     1681 ttataacaca gatgtgattc ctgttctttt caaaaaaggc aaacaaatta ttaaagcagg
     1741 agactcttac aaaggaagat tagctgggcc agtatcttat agctatggta attacaaggt
     1801 ctttgttgat gacagcaaaa acatgccaag tttaatggat ggtcatctaa aacctgaaaa
     1861 aacaaacttg caaaaagacc ttagcaagtt aagcattgct tcttacaata ttgaaaactt
     1921 ctcagccaat ccttcttcaa ctaaagatga gaaggtcaaa cggattgccg aatcctttat
     1981 tcatgatctg aatgctccag acattattgg attaattgaa gtccaagata ataatgggcc
     2041 gactgatgat gggacaacgg atgcgacaca aagcgcacaa cgcctcattg atgctattaa
     2101 aaaactaggt ggcccaactt atcgttatgc tgatattgct ccagaaaata atgttgacgg
     2161 aggtcaacca ggtggtaata ttcgaacagg attcctttat caaccagagc gcgtcagcct
     2221 ttctgataag ccaaaaggcg gtgctcgtga tgctctaact tgggttaatg gagaattaaa
     2281 ccttagtgtt ggtcgaattg atccaactaa cgccgcttgg aaagatgttc gtaaatcact
     2341 agcagcagaa tttatcttcc aaggtcgtaa agtcgttgtt gttgcaaatc atttgaactc
     2401 taagcgtggg gataatgctc tttatggtcg tgtgcaacca gtcactttta aatctgagca
     2461 aagacgtcac gtcttggcta atatgctagc acaatttgcg aaagaagggg caaaacacca
     2521 agctaatatt gtgatgctag gtgactttaa tgattttgaa ttcacaaaga cgattcaatt
     2581 aatcgaagaa ggtgatatgg ttaacttggt gagccgacat gatatttcag atcggtattc
     2641 ttattttcac caaggcaata atcagaccct tgataatata ttagtttcac gccatttact
     2701 tgatcactac gaatttgaca tggttcatgt gaattcccca tttatggaag cgcacggacg
     2761 cgcatcagat catgatccat tgttacttca attatcattt tctaaagaaa atgataaggc
     2821 agagtcttct aaacaaagtg taaaagctaa aaaaacttca aaaggaaaac tgttgccaaa
     2881 aacaggagat agtcttgttt atgtgataac gctactagga acggctagtt tattagtgcc
     2941 tattttatta ttgactaaag gcaaaaagga atcatagcga tttgaagaat tggagttcat
     3001 aaagagtcgt taaaggcttg atatacttac gttagacctt aagctaaaat ggatgactat
     3061 ataaaaacta agattgataa caaaagaaga taacaataag atagctttac ttgctaaaga
     3121 cttttgtcaa gctaattgtt ttatggtaca atgtaaccgt tgctttgcaa cccttggtgc
     3181 ttagttccct ttcaccaagc atattagaaa cgtagatagc ttttgagagt tatccagacg
     3241 ttaaaggaga aaaaatgaca aaattgactg tacacgacta tgttcacatt gggctagtag
     3301 ctgccttgta tgttgtctta actattaccc cacctttaaa tgcgatttct tatgggatgt
     3361 atcaatttag aatatctgaa atgatgaatt tcttagcttt ttatcatcgc aagtatatta
     3421 ttgcggtgac tcttggatgt atgattgcta acttttatag tttcggactg attgatgtct
     3481 ttgtaggcgg cggttcaact cttatttttg tgaccttagg tgtgattttg tttagtaagt
     3541 atcaaaaaga ttatcttttt aatggtattt ttaataaagc ttttgtttac ttttctttct
     3601 tttttgcaac ttccatgttt actgtagcaa tcgaacttta cttctttgga gctccatttt
     3661 tattaacatg gtttacaact gcgttaggag aattagtatc tctgctcata ggatcactta
     3721 ttattgataa gttgtctcaa agaatttcct tttagtaatg catcatactc aaaagagttg
     3781 ggtcaatacc cttctctttt ttatgttg
//
LOCUS       DAUELZ010000117         3734 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.118, whole genome shotgun sequence.
ACCESSION   DAUELZ010000117 DAUELZ010000000
VERSION     DAUELZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.118"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            74..337
                     /locus_tag="VHI09_001527"
     CDS             74..337
                     /locus_tag="VHI09_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEQ6121383.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            342..2075
                     /gene="ptsP"
                     /locus_tag="VHI09_001528"
     CDS             342..2075
                     /gene="ptsP"
                     /locus_tag="VHI09_001528"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEQ6121384.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNGNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            2260..3687
                     /locus_tag="VHI09_001529"
     CDS             2260..3687
                     /locus_tag="VHI09_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ6121385.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALIAGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLALAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAVKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
BASE COUNT         1130 a          698 c          838 g         1068 t
ORIGIN      
        1 tgctataatc ggttgttgta agggttatca tgtgatggct caaaaataca caacttaaaa
       61 ggagaaccat actatggctt caaaagactt tcacattgtt gcagaaacag gtattcatgc
      121 gcgtccagcg actttgcttg ttcaaacagc tagcaaattt gcttcagaca tcactcttga
      181 ctacaaaggt aaagcagtaa atctaaaatc aatcatgggt gttatgagtc ttggtgttgg
      241 tcaaggtgca gatgttacta tctcagctga aggtgcagat gctgaagatg ccattgcagc
      301 aattgaagaa acaatgacta aagagggatt ggcataagac tatgacagaa atgcttaaag
      361 gaattgcagc ctcagacggc gttgctgttg ctaaagcata tctactagtt cagccggatt
      421 tgtcatttga gactgttaca gtcgcagata caaatgcaga agaagctcgc cttgatgttg
      481 cactccaagc tgcacaagac gagctttctg ttatccgtga aaatgcagta gaaagcttag
      541 gtgaagaagc agcagccgtt tttgatgccc atttgatggt tcttgctgat ccagaaatga
      601 ttagccaggt taaagaaacg attcgcgcaa aacaaacgaa tgcagaaaca ggtcttaaag
      661 aagtgactga catgttcatc accatctttg aaggcatgga agataaccca tacatgcaag
      721 aacgtgcagc ggacattcgc gacgttgcaa aacgtgtgtt ggctcacctt ttaggtgtaa
      781 aacttccaaa tccagctaca atcaatgaag aatccatcgt tatcgcacac gatttgacac
      841 cttcagatac tgctcaactt aacaaacaat ttgtaaaagc atttgttaca aatatcggtg
      901 gtcgtacaag tcactcagct atcatggcac gtacacttga gatcgctgcg gtacttggaa
      961 caaatgatat tacaaaacgt gttaaagatg gtgatgtgat tgccgttaat ggtatcactg
     1021 gtgaagtgat tatcgatcca agcgaagatc aagtacttgc ttttaaagaa gctggtgcgg
     1081 cttatgccaa acaaaaagca gagtggtctc tccttaaaga tgcgcatact gaaacagctg
     1141 atggcaaaca ctttgaattg gctgctaata tcggtacacc taaagacgtt gaaggtgtta
     1201 atggcaatgg cgctgaagct gttggccttt accgtactga gttcttgtac atggattctc
     1261 aagacttccc aactgaagac gaacaatacg aagcttacaa ggcagtgctt gaaggcatga
     1321 atggcaaacc tgttgtggtt cgtacgatgg atattggtgg cgacaaggaa cttccttact
     1381 ttgaccttcc aaaagaaatg aatccattcc ttggtttccg tgctcttcgt atttccatct
     1441 ctgaaactgg ggatgccatg ttccgcacac aaatgcgtgc gcttcttcgt gcctctgttc
     1501 acggacaact tcgtattatg ttcccaatgg ttgcccttct taaagaattc cgtgctgcaa
     1561 aagcagtctt tgatgaagaa aaagcaaact tgcttgcaga aggcgttgcg gttgctgatg
     1621 acatccaagt tggtatcatg attgagattc ctgcagctgc tatgcttgca gaccaatttg
     1681 ctaaggaagt tgatttcttc tcaattggaa caaacgacct tatccaatac actatggcag
     1741 cagaccgtat gaacgaacaa gtatcatacc tttaccaacc atacaaccca tcaatattac
     1801 gtttgatcaa caatgtgatc aaagcagcgc acgctgaagg taaatgggca ggtatgtgtg
     1861 gtgagatggc aggtgaccaa caagctgttc cacttcttgt cggaatgggc ttggatgagt
     1921 tctctatgtc agcaacttca gtacttcgta cgcgtagttt aatgaagaaa cttgactctg
     1981 ctaagatgga agaatatgca aatcgtgcgc ttacagaatg ttcaacagca gaagaagttc
     2041 ttgaactttc taaagaatac gtttctgaag attaacgggt taaagagttg gtttaaacca
     2101 gctctttttt gctagctaaa aatacaatta ctttttggtg tgaaatcatt tgagttatca
     2161 aataattatc agaattttca gatatttagt gtagccaaat cttgaattat atcggaaaag
     2221 tgatagaata agattactat cataaaagga gataaaacgt tggcaaaaca atataaaaat
     2281 ttagtgaacg gtgaatggaa actatcagaa aacgagatta ccatttacgc accagcaaca
     2341 ggtgaagagt taggatcagt tccagcgatg acgcaggcag aggtagatgc tgtttacgct
     2401 tcagctaaaa aggctctatc agattggcgc gccttgtctt atgtggaacg tgcagcttac
     2461 cttcataaag cggctgatat tttagtacgt gatgctgaaa agatcggcgc gattctttca
     2521 aaagaagtag ccaaaggtca caaggcagct gtcagtgaag ttattcgtac cgctgaaatc
     2581 attaattatg cagcagaaga agggcttcgt atggaaggtg aagttcttga aggtggtagc
     2641 ttcgaagccg caagtaagaa gaagattgct attgttcgtc gtgaaccagt tggtttagtt
     2701 cttgccatct caccttttaa ttatcccgtt aacttggcag gttctaaaat tgctccagcc
     2761 cttattgcag gaaatgttgt tgctcttaaa ccaccaacac aaggctctat ttctggtttg
     2821 ttactagcag aagcttttgc agaagctggt attccagcag gtgtctttaa taccattaca
     2881 gggcgaggtt ctgttattgg ggattatatc gttgagcacg aagcggttaa ctttatcaac
     2941 tttacaggtt ctactccaat tggggaagga attggtaaat tagcgggtat gcgaccaatt
     3001 atgcttgagc ttggtggtaa ggattctgct atcgttttgg aagatgcaga tcttgcttta
     3061 gcagcgaaaa atattgtagc cggtgctttt ggttactcag gacaacgttg tacagcggtt
     3121 aaacgcgttc ttgtgatgga caaggtggcg gatcaattgg cggctgagat taaaaccctt
     3181 gttgaaaaac taagtgtcgg aatgcctgaa gacgatgctg atattacacc attaattgat
     3241 acatcagctg ctgattttgt tgaagggttg attaaagatg caactgataa gggagctact
     3301 gctttgacag cctttaatcg tgaaggcaat cttatttcac ccgttctctt tgatcatgtg
     3361 acaactgaca tgcgtttggc atgggaagag ccgttcggcc cagtattacc aattattcgt
     3421 gtaaccactg tagaagaagc cgtcaagatt tctaatgagt ctgaatatgg tttgcaagct
     3481 tctattttta caactaattt cccaaaagct tttggcattg ctgagcaatt agaagttgga
     3541 actgttcacc ttaacaataa aacacaacgt ggaacagata atttcccatt cttaggcgct
     3601 aaaaaatcag gtgcaggggt acaaggagtt aaatattcta tcgaagctat gacaactgtt
     3661 aaatctgttg tatttgatat ccagtaataa aaaaataaac aaaaagttag gttaacttaa
     3721 ctttttgttt attt
//
LOCUS       DAUELZ010000118         3727 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.119, whole genome shotgun sequence.
ACCESSION   DAUELZ010000118 DAUELZ010000000
VERSION     DAUELZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.119"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..1526
                     /locus_tag="VHI09_001530"
     CDS             <1..1526
                     /locus_tag="VHI09_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEQ6121386.1"
                     /translation="GIFYFFDSFVGNRHLADASMLRPVVGIISVDNYDDITDDLSDAD
                     TSKINSFVANFIDEFMESKRIFYRRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEA
                     QDAQRPLTLSIGISFGEENHSQIGQVALENLNIALVRGGDQIVIRENADHTNPIYFGG
                     GSVSTVKRSRTRTRAMMTAISGRIKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIE
                     NSFAVYNPDEMSPDIERAIERLQADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTL
                     SKEFYEQFQNVIVVDHHRRDDDFPDNAILTFIESGASSAAELVTELIQFQNAKKRLNK
                     IQASVLMAGIMLDTKNFSTRVTSRTFDVASYLRSKGSDSVEIQNISATDFEEYKQINE
                     IILQGERLGDSIIVAAGEKNHLYSNVIASKAADTILSMAHVEASFVLVETASHKIAIS
                     ARSRSKINVQRVMEKLGGGGHFNLAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            1523..1975
                     /gene="rplI"
                     /locus_tag="VHI09_001531"
     CDS             1523..1975
                     /gene="rplI"
                     /locus_tag="VHI09_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEQ6121387.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLAITEA"
     gene            2005..3372
                     /gene="dnaB"
                     /locus_tag="VHI09_001532"
     CDS             2005..3372
                     /gene="dnaB"
                     /locus_tag="VHI09_001532"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEQ6121388.1"
                     /translation="MRLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDD
                     FYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSA
                     NAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
                     FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGK
                     TAFVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWN
                     NVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKP
                     ENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDA
                     DIVAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIA
                     QFEER"
     gene            3389..3661
                     /locus_tag="VHI09_001533"
     CDS             3389..3661
                     /locus_tag="VHI09_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEQ6121389.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
BASE COUNT         1252 a          573 c          789 g         1113 t
ORIGIN      
        1 caggaatttt ttattttttt gatagctttg tgggaaacag gcacttagct gatgctagta
       61 tgttaagacc ggttgttggc attatttcgg ttgataatta tgatgatatc actgatgatt
      121 tatcagatgc agatacctca aaaattaatt catttgtagc taacttcatt gatgagttta
      181 tggagtcaaa acggattttt taccgtcgtg ttaatatgga tcgctactat ttctttacag
      241 actttaaaac attaaatgat ttgatggata ataaattttc tgtcttagaa gagtttcgta
      301 aagaagctca agatgctcag cgccctttaa cactcagcat aggaatttct tttggtgaag
      361 aaaatcatag ccagattgga caggtagctc ttgagaatct taatattgcg cttgttcgtg
      421 gtggtgatca gattgtcatt cgtgaaaatg cggatcatac gaatccaatt tatttcgggg
      481 gagggtctgt ttctacagtt aaacgttcaa gaacacgtac ccgtgctatg atgacagcta
      541 tttcaggtcg aatcaagatg gtggacaatg tttttattgt cggacatagg aaacttgata
      601 tggatgcact aggttcagct gttggtatgc aatttttcgc aggtaacatt attgaaaata
      661 gttttgccgt ttacaaccca gatgagatga gtccagatat tgaaagggct attgagcgat
      721 tgcaggctga cggaaagact cgtctaatca gtgtttctca agcaatgggt ttagtgactc
      781 caagatcact tttagtaatg gttgatcatt ctaagatatc tttaacactt tctaaagaat
      841 tttatgaaca gtttcaaaat gttattgtag ttgatcatca cagaagagat gatgattttc
      901 ctgataatgc tatcttaact tttattgaaa gtggagcaag tagtgctgcg gagctcgtca
      961 ctgagttaat tcaatttcaa aatgctaaaa aacgtttaaa taagatccaa gccagtgttt
     1021 taatggcagg tattatgctt gatactaaaa atttttctac gcgagtgaca agtcgtacct
     1081 ttgatgtcgc tagttaccta agaagcaaag gaagtgatag tgttgaaatt caaaatatat
     1141 ccgcaacaga ttttgaagaa tataagcaaa taaatgaaat tattttacaa ggagaacgtc
     1201 ttggtgacag tatcattgta gctgcaggag aaaaaaatca tctttatagc aatgttattg
     1261 ctagtaaagc tgcagatacg atactgtcaa tggctcacgt tgaggctagc tttgtattag
     1321 ttgaaacagc ttctcataag attgctattt cagctagaag tcgtagtaaa atcaatgttc
     1381 aacgtgtgat ggaaaaatta ggtggaggag gccactttaa tcttgctgcc tgtcagttaa
     1441 cggatatcag tcttccccaa gcgaagcact tattattgaa aactattaat atgacaatga
     1501 aagaaacagg agaagtagaa tcatgaaagt tattttttta gcagatgtaa aaggaaaagg
     1561 taaaaaagga gaaatcaaag aggtcccaac aggctatgct caaaatttcc ttattaagaa
     1621 aaatttagct aaagaagcaa ctagtcaaag catcggtgaa ttaaaaggaa aacaaaaagc
     1681 tgaagaaaaa gctcaagctg aaatattagc agaagctcaa gctgtcaagg ctgttttgga
     1741 tgaggacaaa acacgtgttc aatttcaaga aaaagttggt ccagatggac gtacatttgg
     1801 ttccatcaca gctaaaaaga tttcagaaga attgcaaaag caatttggtg taaaagttga
     1861 taaacgtcat attgttttag atcatcctat ccgcgccatt ggtctaattg aagttcctgt
     1921 gaaattacat aaggaagtaa cagctgagat taaattggct atcacagaag cataatataa
     1981 aaaacatcac aagatttgga ggttatgagg ttgcctgaag tagctgaatt acgagttcaa
     2041 ccccaagatt tactagcaga gcaatctgtt cttgggtcaa tctttatctc acctgataag
     2101 ctgattgcag tgagagaatt tatcagtcca gacgattttt ataagtacgc tcataaaatt
     2161 atctttcggg caatgattac tctcagcgat cgtaatgatg ccattgatgc aaccactata
     2221 agaacaatcc tagatgatca agatgatctg caaagtattg gtggcttatc ctatattgtt
     2281 gaactagtta atagtgtccc aactagcgct aatgcagaat attatgctaa aattgtagct
     2341 gagaaagcta tgttgcgtga tattattgct aggttgacag aatctgtcaa cctagcttat
     2401 gatgaaattt taaaaccaga agaggttatc gctggagttg agagagcttt aattgaactc
     2461 aatgaacata gtaatcgtag tgggtttcgc aaaatttcag atgtgttaaa agttaattac
     2521 gaggctttag aagcacgttc taagcagact tcaaatgtta caggtttacc aactggtttt
     2581 agagaccttg acaagattac aacaggttta cacccagatc aattagttat tttagctgct
     2641 cggccagcag tggggaagac tgcctttgtt cttaatattg cgcaaaatgt ggggactaag
     2701 caaaaaaaga ctgttgctat tttttctttg gaaatgggtg ctgaaagttt agtagatcgc
     2761 atgcttgcag cagaaggaat ggttgattcg cacagtttaa gaacagggca actcacagat
     2821 caggactgga ataatgtaac aattgctcag ggagctttgg cagaagcacc gatttatatt
     2881 gacgatacgc ccgggattaa aattactgaa atccgcgcaa gatcacggaa attgtctcaa
     2941 gaagtggatg gtggtttagg tctcattgta attgactact tacagttgat tacaggaact
     3001 aaacccgaaa atcgtcagca agaggtttca gatatttcaa gacagcttaa aatcctagct
     3061 aaagaattga aagtaccagt tattgcccta agccagcttt ctcgtggcgt tgagcaaagg
     3121 caggataaac gaccagtttt atcagatatt cgtgaatcag gatctattga gcaggatgcc
     3181 gatattgtag ccttcttata ccgggacgat tattaccgta aagaaggtga tgatgctgaa
     3241 gaagctgttg aagataacac aattgaagtt atcctcgaga aaaatagagc tggggcgcgt
     3301 ggaacagtca aactgatgtt ccaaaaagaa tacaacaaat tctcaagtat agcccagttt
     3361 gaagaaagat aagaaagaga gacaaaaaat gagcgatgca tttacagatg ttgcaaaaat
     3421 gaaaaaaatt aaagaggaca taagagccca tgaaggacag ttagtagaat taacccttga
     3481 gaatggacgg aaacgggaaa aaaataaaat tggtaggctt attgaagttt acagttcact
     3541 ctttatcatt gagtatagtg atagctctga tactccagga gcaattgata attcttatgt
     3601 cgaatcatac acctattcag atattttgac agaaaaaaca ttaattcgtt atttagattg
     3661 atttttgcct catagaggct ttttctttac aataaattga tataattatt agcgatagaa
     3721 gattatt
//
LOCUS       DAUELZ010000119         3583 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.120, whole genome shotgun sequence.
ACCESSION   DAUELZ010000119 DAUELZ010000000
VERSION     DAUELZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.120"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            114..1385
                     /locus_tag="VHI09_001534"
     CDS             114..1385
                     /locus_tag="VHI09_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ6121390.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVTVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            1385..2095
                     /locus_tag="VHI09_001535"
     CDS             1385..2095
                     /locus_tag="VHI09_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121391.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            2107..3327
                     /locus_tag="VHI09_001536"
     CDS             2107..3327
                     /locus_tag="VHI09_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121392.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGEGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
BASE COUNT         1254 a          539 c          729 g         1061 t
ORIGIN      
        1 ataagtcatt atcttgtggc ttgttttcga ctaagggcta agaatttctt gacaacaata
       61 taaaaaggtc atatacttgt cttatacaaa tagtacaaaa ggggaggata gaaatgtcta
      121 aaagaggaaa aattaaaata acaacgaaaa caaagcttat tacagctagt gttataacgc
      181 tagtattaat tataactgga gtagtcttgt ggaaacaaca acaaaatacg ctaacagctg
      241 atatcgctaa agaaccttac tctactgtta gtgtaactga agggagtatt gcttcttcga
      301 ctttactctc aggtactgta aaggctttat cagaggaata tatttatttt gatgctaata
      361 aaggaaatga tgcaactgtt acagttaaag taggtgatca ggtaacgcag ggccagcaat
      421 tagttcaata taatacaaca acagctcagt cagcttatga tactgctgtt aggagtctta
      481 acaagattgg ccgacaaatt aatcatctta aaacatacgg agttcctgct gttagtacag
      541 aaactaataa agatgaagct accggtgaag agacgacaac aacagttcaa ccatcagctc
      601 agcaaaatgc taattataaa cagcagctgc aagatttaaa tgatgcttat gcagatgcac
      661 aagcagaagt aaataaagcg cagatagcgt taaatgatac agtagttatc agtagtgtct
      721 ctggaactgt tgtggaagta aataatgata ttgatccttc ttcaaagaac agtcaaacac
      781 ttgttcacgt agcaaccgaa ggacagcttc aggtgaaagg aacattgaca gagtatgatt
      841 tagcaaacgt taaggttggt cagtctgtaa aaattaagtc taaagtttat tctaatcaag
      901 aatggactgg aaaaatatca tatgtttcaa actatccaac tgagtctaat gcaggttcaa
      961 caacgccagc aggtagcact ggagcgggaa gttctacagg agctgcctat gattacaaga
     1021 ttgatattat aagtcctctc aaccagctta aacaaggctt cactgtttct gttgaggttg
     1081 ttaatgaagc aaaacaggcc ttagttcctt taacggctgt tattaagaaa gataaaaaac
     1141 actatgtttg gacttatgat gatgctactg gcaaagccaa aaaagtagag gtgacacttg
     1201 gaaacgcaga tgcacaacaa caagaaattc ataaaggagt aactgttggt gacattgtta
     1261 ttgccaatcc agataaaaat atcaaaccgg ataaaaaact agaaggggtt atttcaatag
     1321 gtaccaacac aaaaccggaa aaagattctc aatcaaagaa taaaaaatca ggggtggata
     1381 aatagtgtcg catgaaaaaa aacaattaat gcaattatca aatattgtga agagctatca
     1441 aaatggggat caagttctta aagttttgaa aggtattaat ttaacagtat atgagggtga
     1501 atttttagct attatgggtc cgtctggttc gggaaaatca acactaatga atattattgg
     1561 tttattagat cggccaacat ccggagatta cacattacat aatactaaaa tagaaatcct
     1621 taatgatcgt gaattagcaa aagtacgaaa tgatgaaatc ggctttgtct ttcaacaatt
     1681 ctttctgtta gctaaattaa ctgcattaca aaatgttgag ttgcctctta tttatgctgg
     1741 tgtaaacgtc tcaaaaagac gtgaacaggc taagcaattt cttgaaaaag taggacttgg
     1801 acgacgtatc aaacatctac cgtcagaact gtcaggaggt caaaagcaaa gggtggctat
     1861 cgcacgagct ttagtaaatg acccatctat tattttagcg gatgaaccaa caggtgcact
     1921 agatactaaa acaggtcagc aaattatgga actcttgaca gagttaaaca aagaaggtaa
     1981 aacaattatt atggttactc atgaaccaga aattgctgat tttgcaactc ggaaaattat
     2041 tattcgtgat ggtgatatca caacggatac tacagctagt gttgttattg actaggagga
     2101 aaaataatgg aaaattggaa attcgctctt agctcaattt gggggcataa gatgcgctca
     2161 atcctgacaa tgttgggaat tatcatcggt gtagcagcag ttgtgattat tatggggttg
     2221 ggaaatgcta tgaaaaatag tgtaacaagc acattttcta gcaagcaaaa agatattcaa
     2281 ctctattttc aagaaaaagg agaagaagaa gacctgtatg ccggattgca cacgcatgaa
     2341 aataatcacg aagtgaagcc agagtggtta gaacaaattg ttaaagatat cgatggtatt
     2401 gattcttatt actttaccaa ttctgctact tctactattt cgtatgaaaa aaagaaagta
     2461 gacaatgctt cgatcatagg ggtaagtaaa gattatttta atataaaaaa ctatgatatc
     2521 gtagctggtc gtacactaac agataatgat tattctaatt tttcgcgtat cattctttta
     2581 gatacagtat tagccgatga cctatttggc gaaggtaatt ataagtcagc tttaaataaa
     2641 gtggtaagtt tatctgataa agattattta gttattggtg tttataaaac tgatcagacg
     2701 ccagtttctt ttgatggatt atcaggcggc gctgtcatgg caaatactca ggtagcttca
     2761 gagtttggca ccaaagaaat aggatctatc tacattcatg tgaacgatat tcaaaacagt
     2821 atgaacttag gaaatcaagc agctgatatg ctaaccaaca tatcgcatat taaagatgga
     2881 cagtacgctg ttcctgataa tagcaaaatc gttgaagaaa tcaatagtca gttcagtatc
     2941 atgacaacag tcataggttc aattgcagcg atctctcttt tagtgggagg aattggtgtt
     3001 atgaatatta tgttggtatc tgtcacagag cgaactaggg aaattggttt acgtaaagca
     3061 cttggtgcaa cacgcctgaa aattttatca caatttttga ttgaatccgt tgttctaaca
     3121 gttcttggag gcttgatagg attattatta gctcaattaa gtgtaggggc tttaggaaat
     3181 gcgatgacat taaaaggagc ttgtatttct cttgatgtgg ccttaatagc agttttattt
     3241 tctgcctcaa taggtgtctt ttttggaatg ttgccagcta ataaagctag taagttagat
     3301 ccaattgagg cattacgtta tgaatagaat agttcgtatc aattaatcta tagacgctga
     3361 ctagtttaac gtctgattat atctattttt tgtattagca cagtagttat atcattgaag
     3421 tgttagtgca ttttttgagg tgataatatc tagcaaagct atcattggta tgatgatgtg
     3481 gatagcgtat aagtgataca tctcttattt ctagctgaac ttcggttcgg ctaggaagtt
     3541 gtcaggcaat gagttgttta aaataaagga gattagttca atg
//
LOCUS       DAUELZ010000120         3464 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.121, whole genome shotgun sequence.
ACCESSION   DAUELZ010000120 DAUELZ010000000
VERSION     DAUELZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.121"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            77..736
                     /locus_tag="VHI09_001537"
     CDS             77..736
                     /locus_tag="VHI09_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ6121393.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            758..3040
                     /locus_tag="VHI09_001538"
     CDS             758..3040
                     /locus_tag="VHI09_001538"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEQ6121394.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKL
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDRYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYTHGLQDWNVKPRQVYEIFNALPSTINKHLFLHQGEHVYMHNRQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRAFKAALFEGKANQALVDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSRESLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(3120..3341)
                     /locus_tag="VHI09_001539"
     CDS             complement(3120..3341)
                     /locus_tag="VHI09_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6121395.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
BASE COUNT         1105 a          681 c          609 g         1069 t
ORIGIN      
        1 caaagggcat tagcaaatgt taatttctct tgaagataca aattatacat tggtaaaaga
       61 atatttggag aaaagcgtga atcatcacat tttgcaacgg tatattgata atcataactt
      121 tcctatcatt gaaaaaagtt atcacaagta cttgactttt gaaagtctcg aagaggactt
      181 cacttatatt ttaaaagacg gcattgtgaa acagagtgtt ctttcaaaat atggaatgga
      241 atttaatttg agatatgtta caggacttga aattacttct gttctcaaca ctggttattc
      301 aaaagatatg ggagagcctt ataatgttcg catcgagtca gagaaagcta gtttttacaa
      361 ggttcgccgc tcagcatttt taaaagatat aaacgaagat attgaactac aaggatatgt
      421 gaaggacttt taccataatc gtcttcaaaa atccatgaaa aaaatgcaat gtatgttaac
      481 taacggccgt atcggtgcca tttctactca aatatatgac ttaatgaccc tatttggcga
      541 agaactacca aatggtcaaa ttttaattaa ttttgtcatt actaatgagg aactcggtaa
      601 attctgtggc atttctaccg ctagcagcgt cagccgtatt ctaaaacagt tgaaagaaaa
      661 aaacatcatt cgcattgata aacaacatat tatcattacc aatcttgata aattaaaaga
      721 taatatcgtt ttctaagcca ctagaaagga aactcctatg cgctataatc aattttccta
      781 tattccaact agtctagaaa gagcggctga agaattaaaa gaattgggct ttgatctgga
      841 tctgcaaaaa actgccaaag ctaacctaga aagcttttta cgtaaactct tcttccacta
      901 ccctgatagt gattatcctt taagtcatct aatcgccaaa aatgacatgg acgcattgag
      961 cttttttcaa tcagagcaag agttatccaa agaggttttt gatttactgg ccctgcaagt
     1021 ccttggcttt attcctggag ttgattttac agaggccgat gctttccttg ataaactggc
     1081 ctttcctatc cactttgacg aaacagaaat tatcaagcat atccaccact tattagcgac
     1141 ccgttgtaag tctggcatga ccctgattga tgatttggtc agccaaggaa tgcttactat
     1201 ggacaatgat taccacttct tcaacggtaa atcattagct acctttgaca cgtcacagct
     1261 gattcgagaa gttgtttacg ttgaagcacc tcttgatacc gatcaagatg gccaacttga
     1321 tcttattaag gttaatatca ttcgtcctca atctcaaaaa ccattaccaa ctttgatgac
     1381 acctagtcca taccatcaag gtatcaatga agtagccaac gacaaaaaac tttatcgtat
     1441 ggaaaaagag ttagtcgtta aaaaaaggag acaaatcact gtcgaagaca gggattttat
     1501 tcctcttgag acacagcctt gcaaacttcc tataggtcaa aatctagaaa gctttagcta
     1561 cattaactcc tatagcctca acgattattt cctcgcacgt gggtttgcta atatctatgt
     1621 ttcaggtgtg ggaactgctg gttccactgg ctttatgacg agtggagact atgctcaaat
     1681 tgaaagcttt aaagccgtta ttgattggct aaatggaaga gccacagcct acaccagcca
     1741 ctcaaaaacc caccaagtga gagctgattg ggcaaatggt ctagtttgta cgactggaaa
     1801 gtcctacttg ggaactatgt ctactggttt agccactaca ggcgtagacg gattagccat
     1861 gattattgca gaatcagcta tttcttcttg gtataactac taccgcgaaa atggcttagt
     1921 ttgtagtcct ggtggctatc ctggagagga tctagatgtc ctaactgagc tcacctactc
     1981 gcgtaattta ttggctggcg attatcttcg ccataatgat cgttatcaag aattgctaaa
     2041 ccaacagtct caggcgttag accgacaatc tggtgattat aatcaatttt ggcacgaccg
     2101 caattactta aaaaatgctc atcaaataaa gtgtgatgtc gtttatactc atggcttaca
     2161 agactggaat gtcaaaccaa gacaggtcta tgagattttc aatgcgcttc cttccacaat
     2221 caacaagcac ctctttttac atcaaggtga gcatgtttac atgcataatc ggcaatccat
     2281 tgattttcgc gaaagcatga atgccctttt atgtcaaaaa ctcttaggcc tagctaatga
     2341 ttttagcctt cctgaaatga tctggcagga taacacctgc ccacaaaact ggcaggagcg
     2401 taaagtcttt ggaacctcaa ccatcaaaga gcttgacctc ggtcaggaac tgcttttaat
     2461 tgataatcac tatggtgaag atgaatttaa agcttacggt aaagatttcc gtgcgttcaa
     2521 agcagctctc tttgaaggca aggccaatca agccctggtt gatattttac tggaagagga
     2581 cttacctatt aacggtgaaa ttgtcctaca gttaaaagtg aaatcaagtg aaaataaagg
     2641 acttctatct gcacaaatcc ttgactatgg taagaaaaaa cgcttgggcg atcttcctat
     2701 tgctctcacc caatcaagta ttgacaatgg gcaaaatttc tctagagaat cccttaaaga
     2761 gctaccattt agagaagatt cttaccgtgt tatttcgaaa ggtttcatga atttacaaaa
     2821 tcgcaataat ttgtcgtcaa ttgaaactat tcctaataat aaatggatga ctgttagact
     2881 tcctttgcaa ccaaccatct atcatctaga aaaaggagat acccttcgag tcatcttata
     2941 cacaaccgat tttgaacata ctgtccgtga caacagcaac tatgctttaa ctatcgatct
     3001 cagtcagtct caattgattg tgccaatagc atcaaattaa caaagaaaag tgcttagcaa
     3061 tataactaag cacttttttg ttaattggtc ttactaatag caaacagaaa acgatcgtct
     3121 tagttcataa attccgaatc tattcctaaa aaaatatcat caacggaaac cgctaatgct
     3181 tgtgcaatac cttttagctt ctcatatttt gctcgcctta gctttttagt atctttttca
     3241 tacctagcaa tcgttctgac agaaatacct gttaagtgag acaattcctg ctgtgtcatt
     3301 ttctttgttg ttcttaactc ttttaaagtt gatttggtca caattttccc tccacactaa
     3361 aatgagatta gttaatcatg ttaagtttat tataaacttc ggtttttatg aagtcaagtt
     3421 tttagagagt ttttagacca tcttttacga taccttttgc ttta
//
LOCUS       DAUELZ010000121         3312 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.122, whole genome shotgun sequence.
ACCESSION   DAUELZ010000121 DAUELZ010000000
VERSION     DAUELZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.122"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            522..1871
                     /locus_tag="VHI09_001540"
     CDS             522..1871
                     /locus_tag="VHI09_001540"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEQ6121396.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKDEFARILTAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDK
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
     gene            complement(2220..3312)
                     /locus_tag="VHI09_001541"
                     /pseudo
     CDS             complement(2220..3312)
                     /locus_tag="VHI09_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1088 a          591 c          680 g          953 t
ORIGIN      
        1 gcttaaaata atagctctgc cgtatgtaaa ataatgttca atacatggca caaagcatct
       61 tttggcgagc ttttaaaagg gcttgctttt taagtattca aatgattgcc atttttagat
      121 aatgagaata agtaatgtta agaagatagc atgagaaagt atttacaggt gattggggct
      181 tcaattgcct ttgcgggtgt ggctttatgc attgatcata tgtatgatgg ggttattacc
      241 cgtattgaaa tgataaaatc cttaaaataa gctattaagg taaagcggcc tcagggcctt
      301 ttttcatgat atttttaggg aatatgaaat cctattttct cttaaaaaac cactatgatg
      361 agaatatgct tgcattgaca cattttttat gtctgaaaaa acgaaaacct tttcaaaaaa
      421 cctagctttt tcacaggtga aaaccactaa aaaccattga aatattagat agaaaaggtt
      481 atcatactaa tgaaacttaa aattttaagg gagtgaaact catgtcacat attacatttg
      541 attattcaaa agtcctcgag tcatttgcgg gacaacatga aattgatttt ttacaaggcc
      601 aagtcacaga ggccgacaag ttattgcgtg aaggaactgg accaggttca gatttcttgg
      661 gctggcttga tcttccagaa aactatgata aagatgaatt tgcacgtatc ttgactgctg
      721 ctgaaaaaat taagtcagac agcgaagttc ttgtggtgat cggtattggt gggtcatacc
      781 ttggtgccaa agctgctatc gatttcttaa accatcactt tgctaacttg caaactgcca
      841 aagagcgcaa agcgccacaa attctttacg ctggaaactc tatttcatca acttacttag
      901 cagaccttgt agagtacgtt caagacaaag agttctcagt aaacgtgatt tctaaatcag
      961 gaacaactac tgagccagcg attgctttcc gtgtcttcaa agaacttttg gttaaaaaat
     1021 atggccaaga agaagctaac aagcgtattt acgctacaac agacaaagtg aaaggtgctg
     1081 ttaaggtaga agctgacgct aacaactggg aaacatttgt ggtgccagac aacgttggtg
     1141 gtcgtttctc agtcttgact gctgttggtt tgcttccaat tgctgcatca ggtgcagata
     1201 tcacagctct tatggaaggt gcaaatgcag cgcgtaaaga cctttcttca gacaaaatct
     1261 ctgaaaacat tgcttaccaa tacgctgctg ttcgtaatgt gctttaccgt aaaggctaca
     1321 tcactgaaat cttggctaac tacgagccat ctcttcaata ctttggcgaa tggtggaaac
     1381 aattagccgg tgaatcagaa ggaaaagacc aaaaagggat ttacccaact tcagctaact
     1441 tctcaacaga cttacactca cttggtcaat ttatccaaga aggttaccgt aacctctttg
     1501 aaacagttat tcgtgttgat aaacctcgta aaaatgttat cattcctgaa ttagcagaag
     1561 acctagatgg acttggttac cttcaaggta aagacgttga tttcgttaac aagaaagcta
     1621 cagatggtgt gcttcttgcc cacacagatg gcggcgtgcc aaacatgttt gtcactcttc
     1681 cagctcaaga cgaatttact cttggttaca ctatttactt ctttgagctt gctatcgctg
     1741 tttcaggcta catgaatgct gttaacccat ttgatcagcc aggtgttgaa gcttacaaac
     1801 gtaacatgtt tgcgcttctt ggaaaaccag gttttgaaga attatcagca gagcttaacg
     1861 ctcgcctcta ataaacagat agtcaaaaag acttcttcct tgtacaagag cttaaaagtt
     1921 agccaagcaa tctaagaatt ggggtggcac ttccatcatc aagtggatac aaggttaagt
     1981 aaaagcgata ataaagtgcg cttccagaac atgggagcgc actttattat gccaaatttc
     2041 agataaaacc ttcaaaaaag ggaagaagat cctacaagag gagttgaacc gctaaaacag
     2101 ttccagtgct gtttctaggc gaatagcaat ggttaaaaag agagcttatc aaggttgcaa
     2161 gtgacaacct taaagacccc cctagactgt gaaagtatct aggagggact gtcaaaaaat
     2221 taagaaggaa ctctccaaag ttcttctaaa cgtttttggt agtggttctg gtgcaaggct
     2281 aagatggttt tgattaaatc ttcgcgctgc tgttggatac agtccaaact caaaccaaac
     2341 aagcgggtct ctttgggaaa aacaccgagt tcttttaaga aaggcaagag cttctgactg
     2401 gtcacaacca gatctacagg tttatcatag ggataaatat tatcagataa gatattgacc
     2461 cgtgacagtt caactttgta agaagggata gttgaggtga tgaaattagc tataacgtta
     2521 ccaacagttt cttggctctc aataacagta atattgattg gagcaataga gctttccaag
     2581 atctgctcaa ggtataaaca catcaagtgt aagtgaccag aactaatact gccttgacgg
     2641 cctgtgtcat aaagccaatc tttgataacg gtttcaagct tatctagtag tagcatgttg
     2701 ccttgatatt gatcaaaata ataaccatca aaggtaatta aagtttgtag attgtagaga
     2761 gttcttttga aaaaaggaat taagttaggg atatgctcat gaatatcgga atactttcca
     2821 actagtgcct gaagacgatg gattagtaaa tgataatcgg gatctttggt gattaaatgg
     2881 taaagttgtt gtcgctgatg acttgtccag tacccactca agaaggagtt atcagtcgtt
     2941 aaataaatta aaaagagata gtcaaaatca tcagacgaaa atgtgacacg agttcgaggt
     3001 gctagctcat ccacggcaat ggttttgata ttgtcaaaaa taggcaagga tttgagatgt
     3061 tcaaaaagag ctaaatgagg caaatgaaca ggatagcgat ggcgcttcca agtcaacatc
     3121 aacagcacat caaaaaacaa gaagcgtcta tctaaaaagg cagaaggttg taagtttttt
     3181 gtgctgcaaa gataaaagca tgaatcgttt caatgtctgc aggttgtata tcatataata
     3241 taatgccgta ctctttatgt aaaagagcaa tcaaataccg tattttaagt tcatctccga
     3301 taacagtatt gt
//
LOCUS       DAUELZ010000122         3240 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.123, whole genome shotgun sequence.
ACCESSION   DAUELZ010000122 DAUELZ010000000
VERSION     DAUELZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.123"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            89..1507
                     /locus_tag="VHI09_001542"
     CDS             89..1507
                     /locus_tag="VHI09_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ6121397.1"
                     /translation="MDKKIQRFSCTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMI
                     VVKPEDQPRHYRSVQSSFEMDLPDNPMTYTSVPDALGEDGIWAEAGVNEANVAMSATE
                     TITTNSRVLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVA
                     FSDKHDIWWLETIGGHHWIARRVPDDAYVTNPNQFGIDHFEFNNPEDYLCSADLKDFI
                     DTYHLDLTYSHEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPRSFALAWC
                     QKPYRKITVEDVKYVLSSHYQDTVYDPYGSEGTPVSKKIFRPIGINRTSQTAILHIRP
                     NKPQEIAAIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAAL
                     ADPHYNHHETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQ
                     TLLNKILFDASNLMTNRFSLSD"
     gene            1659..3206
                     /locus_tag="VHI09_001543"
     CDS             1659..3206
                     /locus_tag="VHI09_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEQ6121398.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGIVGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDADAFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGAGHDHHHEEADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAESFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
BASE COUNT         1070 a          610 c          679 g          881 t
ORIGIN      
        1 gataaggagg tgctgctgga attatgaaaa tttttgtatc cagttcaatg gatatttgat
       61 ataatcgtct taaaaaggag gtcgtgaaat ggataaaaaa atacagcgtt tttcatgtac
      121 aactatactc gtcggtaaaa aggcatctta tgatggctca accatggttg ctcgaacaga
      181 agattctcaa aatggagatt tcacgcctaa aaaaatgatt gtggtgaaac cagaagatca
      241 accgagacat taccgttcgg tgcaatcttc ctttgaaatg gacttaccgg acaatcccat
      301 gacttacacg tctgttcccg atgcgcttgg tgaagatggt atctgggcag aagcaggcgt
      361 gaacgaggct aatgtggcca tgtcagcaac tgaaaccatc actaccaatt ctcgtgtttt
      421 aggggcagat cctctagtag cttctggtat tggtgaggag gacatggtaa ccttagtgct
      481 tccttatatc cgttctgcgc gtgaaggggt tttgcgcctt ggtgccattt tagaagacta
      541 tggcacttac gagtcaaatg gtgtggcttt ttcagataaa catgacattt ggtggctgga
      601 aacgattggt ggtcatcatt ggattgcaag acgggtgcca gatgatgctt atgtcaccaa
      661 tcccaatcaa tttggcattg atcattttga atttaacaat cctgaagatt acctttgctc
      721 agctgattta aaggacttta ttgacacgta tcacttagat ttaacctata gccatgagca
      781 ttttaatccg cgttatgcct ttggtagtca acgtgacaaa gaccgtcact ataatacgcc
      841 acgtgcttgg atcatgcaaa aattcttaaa cccagaaatt gtgcaagacc cgcgcagctt
      901 tgcccttgct tggtgtcaaa aaccttaccg taaaataaca gtagaagatg tcaaatatgt
      961 attgagtagt cactatcagg acacggttta tgacccttac ggttcagaag gaactcctgt
     1021 tagtaagaaa atctttcgcc ctattggcat taaccgtacc agccaaaccg ctatcttaca
     1081 tattcgtcct aataaacctc aagaaattgc ggctatccag tggatggctt atgggtcaat
     1141 gccttttaat accatggtgc cttttttcac acaggtgaag actattccag attattttgc
     1201 caacacttac gaaaatgtgt ctaccgacaa tttctattgg accaaccgtc tgattgcggc
     1261 tttggcagat cctcactaca atcatcatga aactgatttg gacaattact tggaagaaac
     1321 catggcaaaa ggtcatgcca tgttacatgc tgtggaagca cagttattgg ctggtgaaac
     1381 agtcgatttg gaagaggaaa atcaaaagat gagtgactat gtgcaaggag aaacgcagac
     1441 gctgttgaac aagattctct ttgacgctag caatctaatg accaatcgtt tcagtttgag
     1501 cgattaatag tgaagctaag gctgagcaac tgcttagcct tttttacgat aactttttag
     1561 gaaaagtaat cgcaaaaaga caaaaacttg gaaaaaaaat caaagtatgc tatacttaac
     1621 tggtcaattt ttaaccagtt aataacaaag gaggaatcat gaaaaagaaa attcttttaa
     1681 tgatgagttt aatcagtgtc ttttttgctt ggcaacttac tcaggcaaaa caagtcttag
     1741 cagagggtaa agtgaaggtg gtgacaactt tctatcctgt ttatgaattt acaaaaggga
     1801 ttgttggtaa tgatggcgat gtttccatgc ttatgaaagc aggaacggaa cctcatgatt
     1861 ttgagccttc tacaaaagac attaaaaaaa tccaagatgc agatgcattt gtttatatgg
     1921 atgacaatat ggaaacttgg gtttctgatg tgaaaaaatc attgacatct aaaaaagtga
     1981 ccatcgttaa gggaactggt aacatgctct tggtagcagg agctggacat gaccatcacc
     2041 atgaggaagc tgacaaaaag catgagcata ataaacatag cgaagaagga cacaaccatg
     2101 cttttgaccc acacgtgtgg ttgtcaccat accgtagcat tacggtcgtt gaaaatattc
     2161 gcgacagtct ttcaaaagct tacccagaaa aagcagagag cttcaaagcc aatgccgcta
     2221 cttatattga aaaattaaaa gagcttgaca aagactatac ggcagcactt tcagatgcta
     2281 agcaaaagag ctttgtgaca caacacgcag cttttggtta tatggcactt gactatggct
     2341 tgaaccaaat ttctattaat ggtgtcacac cagatgcaga accatcagca aaacgtattg
     2401 ctactttgtc aaaatatgtt aaaaaatatg gtatcaaata catttatttt gaggaaaatg
     2461 cgtcaagtaa agtcgcaaaa accctagcta aagaagcagg agttaaagcg gctgtgctta
     2521 gtccgcttga aggtttgact gaaaaagaga tgaaagctgg ccaagattac tttacggtca
     2581 tgcgtaaaaa ccttgaaacc ttacgcttaa ccactgatgt ggctggtaaa gaaattcttc
     2641 cagaaaaaga cacgactaag acagtttaca atggttattt caaagacaaa gaagtcaaag
     2701 atcgtcaatt atctgactgg tcaggtagct ggcaatctgt ttacccctat ctacaagatg
     2761 gtactttaga ccaagtttgg gactacaagg ctaaaaaatc taaaggtaaa atgacagcag
     2821 ccgagtacaa agattactac actactggtt ataaaactga cgtggaacaa atcaaaatca
     2881 atggtaagaa aaagaccatg acctttgttc gtaatggtga aaagaaaacc ttcacttaca
     2941 catacgccgg caaagaaatc ttgacctatc caaaaggaaa tcgtggagtt cgtttcatgt
     3001 ttgaagctaa agaaccaaat gctggcgaat tcaaatacgt tcaatttagt gaccatgcca
     3061 ttgctcctga aaaagcagag catttccacc tgtactgggg tggtgacagc caagaaaaat
     3121 tacataaaga gttagaacat tggccgactt actacggttc agacttatct ggtcgtgaaa
     3181 tcgcccaaga aatcaatgct cattaatgct aaaaaagcaa acctaagaaa gattaggttt
//
LOCUS       DAUELZ010000123         3212 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.124, whole genome shotgun sequence.
ACCESSION   DAUELZ010000123 DAUELZ010000000
VERSION     DAUELZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.124"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..158
                     /locus_tag="VHI09_001544"
     CDS             <1..158
                     /locus_tag="VHI09_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ6121399.1"
                     /translation="VKGLELYLPSLPEQEAIGDFFQTLDQQMSQIEDKLTELKALKQT
                     LLNRLFV"
     gene            171..1751
                     /locus_tag="VHI09_001545"
     CDS             171..1751
                     /locus_tag="VHI09_001545"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEQ6121400.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLLAVCDNLEKHFNTFTEAQKIFEDAYQDEGLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGREDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDSHIKKILDAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(2308..2913)
                     /locus_tag="VHI09_001546"
     CDS             complement(2308..2913)
                     /locus_tag="VHI09_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ6121401.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDIILLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(2894..>3212)
                     /locus_tag="VHI09_001547"
     CDS             complement(2894..>3212)
                     /locus_tag="VHI09_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121402.1"
                     /translation="MVVPSPYGDIVYRFIKELINNAIKYGDGKDIRLSLTIQSDIIII
                     EESNQVVEKVHSISYGRGLKSFQETLAAFDGDLELQMDTKQFTIRILLPIDWKLCYED
                     FIN"
BASE COUNT         1078 a          610 c          490 g         1034 t
ORIGIN      
        1 ttgtgaaagg attagagctt tatctaccct ccctccccga acaagaagcc atcggtgact
       61 tcttccaaac cctagaccaa caaatgagtc aaatcgaaga taaattaaca gaacttaaag
      121 cactcaaaca aacattactc aatcgtttat tcgtataaga aaggactcac atggcagaga
      181 aaacaacttc actccgtcaa gcactatggc attcggccga ccaattacgt ggccaaatgg
      241 atgccaatga ctataaaaac tatcttcttg gacttatttt ttacaaacac ttatctgata
      301 aattactctt ggctgtttgt gataatctag aaaaacactt caataccttt acagaggctc
      361 aaaaaatctt tgaagatgct tatcaagatg aaggcctaaa agatgacctc atttctgttg
      421 ttactggtga tttaggttac tttatcgaac caacactgac ctttgaaaaa ctcatccaag
      481 atgtttatca caatactttc caattagaat cattagctca aggttttcgc gatattgaac
      541 aaagtggtga agactttgaa aacttatttg aagatattga cctctactct aaaaaattag
      601 gctctactcc tcaaaaacaa aatcaaacca tttctaatgt catgaaaaca ttaaatgaaa
      661 ttgactttga ggcagtagat ggggatactt taggcgatgc ttatgagtac ttgattggtg
      721 aatttgccag tgagtcagga aaaaaagcag gtgagttcta taccccacaa gctgtctctc
      781 acttgatgac tcagattgtc tttttaggtc gtgaagatca aaaaggaatg accctctatg
      841 accctgctat gggatctgga tcactccttt taaatgctaa gaaatatagt aaccaatccg
      901 ataccgtttc ttactatggt caagaaatca atacttctac ttataacttg gctcgtatga
      961 acatgatgct tcatggcgtt gccatcgaaa accaacactt aagtaacgct gataccttgg
     1021 atgcagactg gccaacagat gagccaacca actttgatgg ggtgttgatg aacccacctt
     1081 attctttgaa atggtcagcg actgctggtt tcttaacgga tccacgtttt tcaagttatg
     1141 gtgtattagc accaaaatca aaagctgact ttgccttttt acttcatggt ttctatcatt
     1201 taaaaaatac aggaactatg gcaattgtct taccacatgg tgttcttttc cgtggagctg
     1261 cagaaggcaa gattcgccaa aaactactcg aacaaggggc tatcgatacc attatcggac
     1321 tcccatcaaa tatcttttat aatacaagca tccctacgac tattatcata ttaaagaaga
     1381 acagaaccaa caaagatgtc ttctttatcg atgcttcaaa agagtttgat aaaggcaaaa
     1441 atcaaaacac aatgacagat agccatatca aaaagatcct tgatgcttat aagagtcgtg
     1501 ataattctga taagttttca tatttagcct catttgatga aatcattgaa aatgactata
     1561 acttaaatat cccacgctat gttgatacct ttgaggaagt cccagttaaa ccactgccag
     1621 aattagctaa acagctcagt gacattgatc aagaaattgc caaaacaaat gccaaactag
     1681 atcaactcat gaaacaacta gtcggaacaa ccaaagaagc acaagacgaa ttggatactt
     1741 ttagaaacta aaagacaaaa aaacaccaca gtagatgatt actactgtgg cgttttattt
     1801 aaaattgagt agtgaacctt tattgagtac ttatcaaaca agattctcct tcttcaccta
     1861 taaatcaaaa tagaatcatc tcatctaact ttactttgtc cgtttctttg ttttatttaa
     1921 aatagttatt tgaagttaca atgatttctc aaacgtttag aaagcttaaa cacggttaag
     1981 agaaattttc aggacaactt ttgatccttc cacaaaaacc atggtgcgaa ccataaatta
     2041 gttccaatcg ttgatatata agcatatatt aacgttctgg gagttgtgaa gctttacgag
     2101 tttttttaga cctggtttaa acaacttttg tttgactaaa tttaaaaagc ttttcatatc
     2161 aggcattgcc ctacttttta taaaagcgtt atctcaatca atttcaatca aaaagaactc
     2221 aaaaacaatg agtttaaatc tgggtattta acaggaaatc cctagcaacg acacaagtaa
     2281 aacttatgtc gtttgattat ctgcaactca gaaatccata aaatacccta attgttcggc
     2341 tttgtagata gcctcttgct tattatttac caaaagcttt gagtagatgt gggtcaaatg
     2401 attaaatact gttctcctac taatttgtaa cgtttcctct atttctttta tagagaatcc
     2461 tttcttactc aaatttaaga tagctatttc ctggtcagtt aaagattcaa cataatcctg
     2521 agattcgatt tgttcaaaat acttttttcc agaatccact ttttttaaga tagaaatgag
     2581 ttgtttaggg tctatatttt tatctacaaa accgtatgct cctacttttt ttgccctctc
     2641 tctatagatt gatttaacat atcctgtcaa cataacaact ttcaaatgag gagtagactg
     2701 tatgagttct tttgctatct ccaatccatt ttctttagaa atatttgtta aattaatatc
     2761 aagcaatata atatcgtact tagacaaatc aatcgtcaca tcgttaaaat gagaagtaat
     2821 cgtatctatg acatctactt catcatactg ttggaataac agttggatac tctttgcaaa
     2881 tagtctgtgg tcatcaatta ataaaatctt cataacacaa tttccaatct atgggaagta
     2941 atattctgat agtaaattgt ttagtatcca tttgtagttc tagatctcca tcaaaagctg
     3001 ctagtgtttc ttgaaatgat ttcaatcctc tgccataact tatagagtgt actttttcca
     3061 ctacttgatt gctctcctca atgataataa tatcagattg aatcgttaaa gataaacgaa
     3121 tgtcttttcc atctccatat tttatagcat tgtttattag ctcttttata aacctataaa
     3181 caatatctcc atatggggat ggcactacca ta
//
LOCUS       DAUELZ010000124         3199 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.125, whole genome shotgun sequence.
ACCESSION   DAUELZ010000124 DAUELZ010000000
VERSION     DAUELZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.125"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            37..1899
                     /gene="cadA"
                     /locus_tag="VHI09_001548"
     CDS             37..1899
                     /gene="cadA"
                     /locus_tag="VHI09_001548"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEQ6121403.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAEAASTHPISKALLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYALLDDIKI
                     ESKRAIKSLHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANIFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(2199..3134)
                     /locus_tag="VHI09_001549"
     CDS             complement(2199..3134)
                     /locus_tag="VHI09_001549"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEQ6121404.1"
                     /translation="MVSTATQIGHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
BASE COUNT          959 a          584 c          681 g          975 t
ORIGIN      
        1 aagattgaat aacttttaat tgcgagggaa ctaaaaatga caatcagaca atggatggca
       61 gaccatcttc atttgatgga gactttagcg tgtttggtat taataataat tggcctagcc
      121 tttttacact catttccaca agtggcttcc gctattttca ttacagcctt cttgattgga
      181 ggatatgcgt ctgcaaaaac gggtatattg gatttggtga agaacaaaca cttgtcagtg
      241 gatattttga tgattttggc agctatcggt gctggaatta tcggctattg gctggagggt
      301 gctctgctta tttttatctt ttcgttgtcc aatacgcttg aagaaatggc catggaaaaa
      361 agtaaggatg ctatttcagc cttgatgtcc ttgacgccag atacagctcg tcaataccaa
      421 gaagatggcc atattttaga agttgagacc agatccttga gtgttggtga ccgtttacaa
      481 gttcgtaaag gcgaagctgt tccaattgac gggcagttgc ttagtccttt tggtcaattt
      541 gatgaatcta tggtcactgg tgagcctatc actgttgata aggcagaagg ccaggatctg
      601 attgggggaa ccattaacca aggacaaacc atagatatgt tggttactat tgaaaatgac
      661 gatactctct ttgccaagat tattaatctg gtggaatctg cccaagaaaa gaaaagcaaa
      721 accgccacct ttatcgaaag cttagaagat ggttatgtca aatttgtgct cgtccttatt
      781 cctgccttta tcctctttag tcactttgtg ctttcttgga cttggttggc tgctttttac
      841 cgagggatga ttctcttaac agtagcttca ccatgtgccc taattgccag ttccacacct
      901 gctagcttgg ctgctatttc tcgtgcagcc agaaagggat tgattatcaa aggaggggat
      961 attgtcgata acatgggaga tattaaggct gttgtcatgg ataaaacggg aacgctcacc
     1021 caaggaaaac cttctgttgt gaatgctcat tatttggaag atgagttgct cgtgaataga
     1081 ctggtaaaag gagcagaggc tgctagtacc catcctattt ctaaagccct tcttgaatac
     1141 actgaaaagt tggagccact gacctttgac cacttagaag aaatttctgg gaaaggtttt
     1201 cagggcttct atcaagggca agaatggcga attggcaaga aaaccttcat tttggaaaag
     1261 gttcaagacc tatcagcttt tgaagaaact attcaagtgg aagaaaatca agggaaaacc
     1321 ctaatctttg tttcacgtga ccatcaattg atagcttact atgccctctt ggatgatatc
     1381 aaaatagaat caaaacgtgc tattaagtct cttcatgcca tgggaatcaa aacagtcatg
     1441 ttaacaggtg accaagaacg aaccgccaat tatgtggcac aaaaacttgg tattgatgaa
     1501 gtggtagcca actgtatgcc tcaagataag gtggctaagt tagcagaatt aaagactaaa
     1561 tatggttttg tggctatggt aggagatggt attaatgatg ctcctgccct tgctcaagca
     1621 gatgtttctt atgctattgg atcaggaaca gatattgcaa tggaaagtgc agacagtgtg
     1681 attatggatg acttgactcg tattccattt tcgattcaac tttcccgcaa aatgaagacc
     1741 attatcaaac aaaatattgt ttttgcctta tctgtgatta ccttattgat tttagccaat
     1801 atttttcagg tagttaactt gccgcttggt gttgttggac acgaaggctc aacgatttta
     1861 gtgattttaa atggcttgcg tttactttct tttaaataat ttagaaactt gcttgaaaac
     1921 ttgaaagtgt ttataataat agtaagactt atgaaagaga aaaccgccgt atgtttacta
     1981 ttatgatcat tggtttagct gtattttcaa taattaaaat cattctattt ttctattttt
     2041 atcagcaatt tgagcgcaag caaaaaagaa aagttgaatc aaacaaccag caaagacagt
     2101 cccaagaaag tgacgctgtc ccttcaaaaa aattcccatt ttaaaaagct tggaaagtgt
     2161 acaaagtgca catttccaag cttttttgac tattaaggtt aatctttgta acgtaagttc
     2221 ccacgaaaat cgtctaagct ttggtagcct ttttctacca taatcgtttt tagttcctta
     2281 gtgacccgtt caaagatagc tggcccttct tgatgaaggg ctgtcccaat ttggaccatg
     2341 ctggcgccac aaagaatatg ttcaaaggca tcgcgtcctg ttttaactcc ccctgtacca
     2401 ataatgtgga tagaaggttt taagcgtttg taaaacgcat ggacattggc tagagcagta
     2461 ggtttgatgt aatctccacc tataccacca aagccatttt taggcttgat gaggacttgc
     2521 tcatcctcaa tgaccagccc atttccgata gaattgacac agttaacaaa ggaaaggggg
     2581 tatttattga aaatcgcagc cgcttgatca aaatgcacaa tgtcaaaata aggaggtaat
     2641 ttgataccaa gaggtttagt gtaataggta aagatgttct ctagaagttg atcagtggtt
     2701 tcaaaatcat aagcaatctg tggttttcct ggaacgttag ggcaagaaag gtttagttcc
     2761 actagaccct cataatctga agccatgatg gcttttaaaa tggtttcggt ttcagtagga
     2821 gatagcccga caacggagag aaagtgaggt ttatgttgcc cggttttggc aagatcactg
     2881 acaaagtcta agtagtagcg aaaaccatta ttagggagtc ccattgaatt gatagaacca
     2941 agacgagtat ctgcgtagcg aggttctgga ttacctggac gaacttcaag cgtccctgtt
     3001 ttggtgacaa aggaagcggc ttgagacttt tcaacctcca taagctcttc ttttgtcatg
     3061 cagtaaacac cagcagcatt catcaaacag ttatcaaaag agaaatgacc aatttgtgta
     3121 gcagtagaaa ccattatttc ctccatattt tagacaaatt ttttctattc taccactagg
     3181 catgaataat agcaagcta
//
LOCUS       DAUELZ010000125         3189 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.126, whole genome shotgun sequence.
ACCESSION   DAUELZ010000125 DAUELZ010000000
VERSION     DAUELZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.126"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..418)
                     /locus_tag="VHI09_001550"
     CDS             complement(<1..418)
                     /locus_tag="VHI09_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEQ6121405.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYQLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVS"
     gene            complement(508..2406)
                     /gene="mnmG"
                     /locus_tag="VHI09_001551"
     CDS             complement(508..2406)
                     /gene="mnmG"
                     /locus_tag="VHI09_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEQ6121406.1"
                     /translation="MTHEFTENYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDADYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            complement(2530..2847)
                     /locus_tag="VHI09_001552"
     CDS             complement(2530..2847)
                     /locus_tag="VHI09_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEQ6121407.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRAKVNQLAFVVENHFDIDDVFWHNIEFHYLASPLEDPKLEMIENASDRFVNGFQSIN
                     WIK"
BASE COUNT          981 a          673 c          507 g         1028 t
ORIGIN      
        1 cagaaacttc aaatgtttga gaaatatctt cacgacgttt ttccgtaata atttgctgaa
       61 tcaaactgtt ttgtataaaa ccctcttcag ttgtaaaaat taattctgca taaggattat
      121 accattctac agcgttggtc tcttgatcaa attgaactac tccaacaggc atattatcaa
      181 gtagcgtttt tagattatct tctgtttgtt catttaagag ctcaatatga gctaagtctg
      241 ataattgata agcttctttt tgataccata aaagagcaac aacaaatagc aaaacgagaa
      301 aaatagctaa taatattaat atttttgatt gcataatact gacacaaagt gccagtagtc
      361 caaataaaat caatcccatc ataatcaaat gaatggtttc aaaacgaaat tttttcatct
      421 tataacctct taagcacaca ttataccata aaaaagccct gaaatacaga gcttccactt
      481 gctttgagat tacaatttca tttccattta atattttcga tgagcttttc cgtttccttc
      541 taaataaatc atcaagatac tgatgtccgc aggattgact cctgaaatac gactagcttg
      601 tccaattgtt tcaggattaa ttttcttaaa tttctgacgc gcctcagttg caatagaatc
      661 aatagcatcc caatcaatat ttgcaggaat acgtttttct tccatgcgtt tcatcttagc
      721 aacctgatct aaagctttgc gaatatagcc ttcgtacttg atttctgttt ctaaaagttc
      781 aatgatttta gcatctaaat cttcagccgc aggaccaaca aaagagactg cagtcgcata
      841 atcaatttct gggcgacgca taaattcttt agctgtcata gcatccgtca atggtttaaa
      901 accaagttcc tgcacacgat cgttggttgc ttttactggt tttaatttga tactgttcag
      961 acgttttaat tcgttatcaa attggttttt cttaatttcg aaggccttcc aacgctcatc
     1021 atcaaccaac ccaatgtcgc gaccaatctc tgtcaaacgc atatcagcgt tgtcatgacg
     1081 taaaatcaaa cgatactcag ctcgcgatgt caacaatcga taaggttcca aggttccttt
     1141 ggtaaccaaa tcgtcaatca taacgccaat ataagcatcg ctccgtttca aaataagttc
     1201 tggcttacct tgaactttca aagcagcatt gattccagca ataagtcctt ggccagcagc
     1261 ctcttcatac ccagaagttc cattagtttg cccagccgta aagagtcctg aaattagctt
     1321 ggtttcaagt gtggcacgta gctggtgagg taaaacaata tcatattcaa tagcataacc
     1381 agtgcgcatc atttcagcct tttcaagtcc tttgatcgaa tgaatcaaat ctttttggac
     1441 atcttctgga agactcgttg acaacccttg aacataaact tcttctgtat cacgcccctc
     1501 tggctctaag aaaagttgat gacgttcttt atcagcaaag cgaacaatct tatcttcaat
     1561 agatgggcag taacgagggc ctacaccttt aacaatgccg gaaaacatcg gtgctctgta
     1621 aaggttctga ttaatgatat catgactagt ttgattcgta taagtcaacc agcagggaat
     1681 ttgatctttg agataatcag catcttttga cataaatgaa aaatgattag gtttgtcatc
     1741 acctggctga atttccgttt gatcgtaatt aatagaagat gctttgacac gaggaggcgt
     1801 tcctgttttg aatctgccaa tttctaagcc gagttttttt aaattatctg ctaaggttac
     1861 tgatgccaaa ctattattag gcccagaaga atatttgagt tctcctaaaa taatttcgcc
     1921 acgcagagct gttcctgttg tcactacaac tgccttagct gcaaattttt gtcctgtggc
     1981 tgttaaaaca cctacaactc gtccatcttc aactagaata tcatcaatca tcgtttggcg
     2041 aagagtcaaa ttagcctgtt tttcaaccgt atgtttcatt tcacgcgcat acagagattt
     2101 atctgcttga gctcggagag cgcgtacagc tggtccctta cctgtattaa gcattttcat
     2161 ctggatatag gttttatcaa tatttttacc catttcgccg ccaagagcat cgatttctcg
     2221 tactactatc cctttggcag atcctccaat agaaggatta cacggcataa aagcaagcat
     2281 atcaaggttg attgttgcca ataaagtttt acaacccata cgactagtgg ccaaactggc
     2341 ttcaacacca gcatgacctg cacctatgac aatgacgtca tagttttctg taaattcatg
     2401 tgtcattttt ttaaaatcct ttatttcagt ctattcattt aaaatcatac gctttagttt
     2461 agccttcaat gcggacaata tgtcctggcc atttagccaa ctcggtttgt aaaaactcag
     2521 gaacaagatt catttgatcc agttgattga ttggaatcca ttcacaaacc tgtcgcttgc
     2581 attctctatc atttctagtt tagggtcttc taaggggctt gccaaataat gaaattcaat
     2641 attatgccag aaaacatcat cgatatcaaa atgattctca accacaaaag ccagttgatt
     2701 aaccttagct cgaataccaa cttcctctaa ggtttcacgt aaaacagttt catgcgtttt
     2761 ttcacctacc aaacttgtac caccaatggt gtaatattgg tcagctgcat ctctagttaa
     2821 aaaattctta ccatttctaa caatcaagca ctagctcgta aactaaagtt atctgacctc
     2881 tagtgattct aaatcccagt cctattagcc atctcatttc tagagctaaa ccataaaaag
     2941 ggccaaaacc ctagaagatt gcaggacaaa aaaacctaca actttcatga gctttgacca
     3001 tcattccgat tgttactatt ttaaaaaaat gattagtgat tgtcaacatt gatttcatca
     3061 cagaccttta cagtaatgcc aatgttttta cagctctgtc aatctattgt aatgttttta
     3121 aaatgattgc aaaagtatca ctatgtctaa tctagcgagg cgagaaataa aaatgtcatc
     3181 agtattatc
//
LOCUS       DAUELZ010000126         3175 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.127, whole genome shotgun sequence.
ACCESSION   DAUELZ010000126 DAUELZ010000000
VERSION     DAUELZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.127"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            220..1236
                     /locus_tag="VHI09_001553"
     CDS             220..1236
                     /locus_tag="VHI09_001553"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ6121408.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     RNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            1269..2168
                     /gene="galU"
                     /locus_tag="VHI09_001554"
     CDS             1269..2168
                     /gene="galU"
                     /locus_tag="VHI09_001554"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEQ6121409.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(2268..2939)
                     /locus_tag="VHI09_001555"
     CDS             complement(2268..2939)
                     /locus_tag="VHI09_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEQ6121410.1"
                     /translation="MTRLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLTPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGALLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
BASE COUNT         1053 a          658 c          584 g          880 t
ORIGIN      
        1 tctttatttt taaaaataag tttccgaact taaataacta aaaagaacag ggcaaagcca
       61 tgacaaccgc tcacctgatg aagcttatta aacaacatta ttaatatagc tagctctctt
      121 gggaacgcct tattagccaa gaccatatac cttttctaat agagctaatc aaggtgttat
      181 gttataatag agtgactagc tcttgaagga gaatttagaa tgacaaaaca aaaagtagct
      241 attttaggac ctggttcttg gggaactgct ctttctcagg ttcttaatga caatggccat
      301 gacgtccgtt tatggggaaa tattcctgat caaattgaag aaataaatac caagcatacc
      361 aatagacact acttcaaaga catcgtgtta gacaaaaaca ttacggcaac tcttgattta
      421 ggtcaagccc tatcagatgt tgatgcggtg ctttttgttg tgccaactaa agtaacacga
      481 ttagttgcta gacaagttgc cgccatttta gatcataaag tagtggtcat gcatgcctca
      541 aaaggtctag aaccagagac ccacgaacgt ttatcaacta tcctagaaga agaaattcca
      601 gctcattttc gtagtgaagt ggtcgttgta tcaggtccta gtcatgctga agaaactatc
      661 gttcgcgata ttactctcat cacggcagcc tcaaaagata ttgaagctgc aaagtatgtt
      721 cagtccctct ttagtaacca ctacttccga ctttatacca ataccgacgt catcggagtt
      781 gaaacagctg gtgccctcaa aaacattatt gctgttggag caggagctct ccatggactt
      841 gggtatggag ataatgccaa agcagcggta attactcgtg ggttagctga aatcacccgt
      901 ctaggcgtca agcttggtgc tgacccgtta acctacagtg ggttatctgg tgttggtgat
      961 ctaattgtaa caggtacttc tgtccattca cgtaactggc gggctggagc tgcccttgga
     1021 cgtggtgaaa aattagaaga tattgaacgc aacatgggta tggttatcga aggcatcgca
     1081 acaactaagg tagcctatga aattgcccaa gacctcggag tttacatgcc aattacaaca
     1141 gctatttata agtccatcta cgagggcgct gatatcaaag aaagcatcct cggcatgatg
     1201 tcaaatgaat ttcgttctga aaatgaatgg cattaagcca tgcgtcacta aaataaaagg
     1261 aggtgctgat gaccaaagtc agaaaagcta ttattcccgc agcaggttta ggcacacgtt
     1321 ttttacctgc aaccaaggcc cttgccaagg agatgctacc tattgttgat aaaccaacta
     1381 ttcaatttat cgttgaagaa gctctaaaat caggtatcga agaaattctt atcgttactg
     1441 gaaaatccaa acgctccata gaagatcatt ttgattctaa ttttgaatta gaatataatt
     1501 tacaggctaa aggaaaaatc gaacttctaa agttagtaga cgagaccact tctattaacc
     1561 ttcactttat tcgacagagt catccaagag gattaggaga cgcagttctt caagcaaaga
     1621 cctttgttgg caatgaacct tttgttgtca tgctaggtga tgatctgatg gatattacaa
     1681 atccaaatgt aaaaccatta accaaacaat tgatcgatga ctatgaagaa actcatgctg
     1741 caactattgc cgtcatgagg gttcctcacg aggatgtctc taactacggt atcattgctc
     1801 ctcaagcaaa agcagtaaaa ggactctata gtgtggatac ttttgttgaa aaaccacaac
     1861 ctcaagatgc acctagtgat ttggcaatta ttggacgtta cttgctcaca ccagaaattt
     1921 ttagtatttt agaaaaacaa gaacctggtg caggcaatga agttcaactg actgatgcta
     1981 ttgataccct caataagaca cagcgtgtct ttgctcgtga gtttaaaggg aaacgctatg
     2041 atgttggcga taaatttggg tttatgaaaa cttctttgga ttatgccctg aaacaccccc
     2101 aagtcaaaga tgacttgaaa gcctacatca tccaactggg caaagctcta gagaaaacaa
     2161 aaccataaaa ataaggaggg aaaatccctc cttatttttt atggtttttg agtcatttca
     2221 tcctactagc catctgagaa gtaacagtgc taaccactca cttattttta taaaaaccca
     2281 agcactaaca caccaaccaa aataatacca tagctcacta aagccaacat tcgcttggtt
     2341 ttcttcactg ttatcactct catcttagtt ggaaaaacta tactcagtaa cgcaccaccc
     2401 acgacaccgc caatatggcc tgccatactc acgtttggca taaaaagatt catcagtaaa
     2461 ttgactacaa ttaacgtttg gtatgacttg cccaaatctt tcaacgcttg gttcttaccc
     2521 aaaaaactca atactacaat agcagcaaaa agtccaaata acgacgtcga tgctcctgca
     2581 gctactgttt caggagtcaa ccaaaaagta aaggcattgc ccataacacc tgataaaaca
     2641 taaagtaata aaaaacggcg tgagccccag aggtcttcga cgatttgacc aacaaaatat
     2701 agtgttaatc cattgacaaa aaaatgacca aagccgatat gaataaatat cggagtcacc
     2761 aatcgccaca actggtctgg cattgctttt actagtaatc caaacatacc tccaacttga
     2821 taaatagctt gagcaccagt tgctaaatgt ccatatacca cttgcattgc tataaaaatt
     2881 aagcctgtta accctaaaag gaaaatagta ataggatatc gttttaataa ccgtgtcatc
     2941 taagataaac ctcctaaaca gcaatatcat aaatcgtttg atttggcaat cgtattggcc
     3001 cgctgataag cttgattgct aacaacatcc actaataaag cttgatcatt ttgacgtttt
     3061 aagtctacat ccatagttcg taaagactag aagagttctt tgagaatctc ttttttcatc
     3121 atcttcttat cataccataa attaggaaaa aggttcaagg ctgacaagaa actag
//
LOCUS       DAUELZ010000127         3168 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.128, whole genome shotgun sequence.
ACCESSION   DAUELZ010000127 DAUELZ010000000
VERSION     DAUELZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.128"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..1676
                     /locus_tag="VHI09_001556"
     CDS             <1..1676
                     /locus_tag="VHI09_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEQ6121411.1"
                     /translation="DNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSF
                     EASDDLEEIKEGAFMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAF
                     RQNGANNLIFMGSKVKTIGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLP
                     ASLKTIREEAFKKNHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALA
                     DGEHFIVDPDKLSSTMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSKL
                     RQGEKQKFLQEAQFFLGRVDLDKAIAKAEKALVTKKATKNGQLLGRSINKAVLAYNNS
                     AIKKANVKRLEKELDLLTGLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDK
                     SGKLIYALDMSDTIGEGQKDAYGNPILNVDEDNEGYHSLAVATLADYEGLDIKTILNS
                     KLSQLTSIRQVPTAAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRG
                     LQSNPKTNRGRHSAILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
     gene            1696..2097
                     /locus_tag="VHI09_001557"
     CDS             1696..2097
                     /locus_tag="VHI09_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEQ6121412.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAQGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEINSESN"
     gene            complement(2277..3152)
                     /locus_tag="VHI09_001558"
     CDS             complement(2277..3152)
                     /locus_tag="VHI09_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEQ6121413.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYCALHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLRESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSGIEVN"
BASE COUNT         1109 a          501 c          610 g          948 t
ORIGIN      
        1 gtgataatgc ttttcgcaat gttgattttc aaaataaaac tttacgtaaa tatgatttgg
       61 aagaagtaaa gcttccctca actattcgga aaataggtgc ttttgctttt caatctaata
      121 acttgaaatc ttttgaagca agtgacgatt tagaagagat taaagaggga gcctttatga
      181 ataatcgtat tgaaaccttg gaattaaaag ataaattagt tactattggt gatgcggctt
      241 tccatattaa tcatatttat gccattgttc ttccagaatc tgtacaagaa atagggcgtt
      301 cagcatttcg gcaaaatggt gcaaataatc ttatttttat gggaagtaag gttaagacca
      361 taggtgagat ggcattttta tcaaatagac ttgaacatct ggatctttct gagcaaaaac
      421 agttaacaga gattcctgtt caagcctttt cagacaatgc cttgaaagaa gtattattac
      481 cagcatcact gaaaacgatt cgagaagaag ccttcaaaaa gaatcattta aaacaactgg
      541 aagtggcatc tgccttgtcc catattgctt ttaatgcttt agatgataat gatggtgatg
      601 aacaatttga caataaagtg gttgttaaaa cgcatcataa ttcctacgca ctagcagatg
      661 gtgagcattt tatcgttgat ccagataagt tatcttctac aatggtagac cttgaaaaga
      721 ttttaaaact aatcgaaggt ttagattatt ctacattacg tcagactact caaactcagt
      781 ttagagacat gactactgca ggtaaagcgt tgttgtcaaa atctaaactc cgacaaggag
      841 aaaaacaaaa attccttcaa gaagcacaat ttttccttgg ccgcgttgat ttggataaag
      901 ccatagctaa agctgagaag gctttagtga ccaagaaggc aacaaagaat ggtcagttgc
      961 ttggaaggag tattaacaaa gcggtattag cttataataa tagcgctatt aaaaaagcta
     1021 atgttaagcg cttggaaaaa gagttagact tgctaacagg attagttgag ggaaaaggac
     1081 cattagcgca agctacaatg gtacaaggag tttatttatt aaagacgcct ttgccattgc
     1141 cagaatatta tatcggattg aacgtttatt ttgacaagtc tggaaaattg atttatgcac
     1201 ttgatatgag tgatactatt ggcgagggac aaaaagacgc ttatggtaat cctatattaa
     1261 atgttgacga ggataatgaa ggttatcatt ccttggcagt tgccacttta gctgattatg
     1321 aggggctcga catcaaaaca attttaaata gtaagcttag tcaattaacg tctattcgtc
     1381 aggtaccgac tgcagcctat catagagccg gtattttcca agctatccaa aatgcagcgg
     1441 cagaagcaga acagttattg cctaaaccag gtacgcactc tgagaaatca agctcaagtg
     1501 aatctgctaa ctctaaagat agaggattgc aatcaaaccc aaaaacgaat agaggacgac
     1561 actctgcaat attgcctagg acagggtcaa aaggcagctt tgtctatgga atcttaggtt
     1621 acactagcgt tgctttactg tcactaataa ctgctataaa aaagaaaaaa tattaaattg
     1681 atttaaggag aaaaaatgat aacacaagaa atgaaagatc ttattaataa ccaattagca
     1741 atggttgcga cggttgatgc caaaggtcaa cccaatattg gtccaaaacg ttctatgcgt
     1801 ctctgggatg ataaaacatt tatttataat gaaaatacag atggtcaaac tcgtattaac
     1861 attgaagata atgggaaaat tgaaattgcc tttgttgatc gtgagcgcct tttaggttac
     1921 aggtttgtag gaactgctga aattcaaaca gaaggtgctt attacgaagc agcgaaaaaa
     1981 tgggcacaag gacgcatggg ggtaccaaaa gcagttggta ttattcatgt cgagcgtatt
     2041 tttaatttac aatctggtgc taatgcaggt aaagaaataa acagtgaaag taactaaaat
     2101 taactgtaac agtatttagg acccttattt taaacgctaa aaaacaagca tactcttttt
     2161 tacagcatag tgactatgtt gtaaaaaggg gtatgcttat taggtgctct taatatttct
     2221 agataactgc acctaataag ttttcaatag acttatagca cattagttga cttcaattag
     2281 ttgacttcaa taccagaaag agaagaacag tgatgttggt gttcgtttaa agaagtatcg
     2341 atttccacag tgactgagtt tatattgtac ctttgacaaa tcagtctaat agattttttg
     2401 caatgttttt cagtggtaga ttctctcaag cagcaatgta tagttgctct atggtcaata
     2461 ccatccatcg accaaacatt aagctgatta acagacacta tatgaggaag ttggctgagt
     2521 tcatggtgca aagcgcaata gtctatcgaa tcaggaacac catctaaaaa aatgttagcg
     2581 gttgctacta actttggaag agctttagat aatataaaag aagcaatggc aattgataat
     2641 aaaggatcaa gaatgtacca aggtttccat tttaagatca gtgacaatat gataatggct
     2701 aaccacccaa ggatatcttc taaaaagtga aggcttaata tttcttcatt ttttgtctgg
     2761 ttagagtgaa tgatgaagct agcaaatcca ttgattataa ttgcaaaaat agctaaaacg
     2821 aacataccgt cataattaac aatagtagga tgccataatt ttggtatcgt ttcaatcatt
     2881 actaataatg acccgctaat aagtattaga tttgttgtta gtgctcctaa aaggctaaat
     2941 cgcttatagc ctagtgaaaa atttggactc tcttttttaa cagcctttct ttctaagata
     3001 gctgagatac caatagcaat ggcatctcca aaatcgtgga cagcatctgc taaaatagca
     3061 cccgaaaaga ataatgttcc aaaaataaat tcaatgaggg aaaaactaag atttaatata
     3121 aaaataatgg tgactttttt acttgctggc ataattttct ccttttgt
//
LOCUS       DAUELZ010000128         3085 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.129, whole genome shotgun sequence.
ACCESSION   DAUELZ010000128 DAUELZ010000000
VERSION     DAUELZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.129"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..464)
                     /locus_tag="VHI09_001559"
     CDS             complement(<1..464)
                     /locus_tag="VHI09_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEQ6121414.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKD"
     gene            complement(627..2159)
                     /locus_tag="VHI09_001560"
     CDS             complement(627..2159)
                     /locus_tag="VHI09_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEQ6121415.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVMVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPILA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGSWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            complement(2337..3068)
                     /gene="aphA"
                     /locus_tag="VHI09_001561"
     CDS             complement(2337..3068)
                     /gene="aphA"
                     /locus_tag="VHI09_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEQ6121416.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
BASE COUNT         1059 a          626 c          469 g          931 t
ORIGIN      
        1 aaatctttat taatagcatc aatcatagca tttgttctta ttttgacagt tttctgatat
       61 cggggattgt tggaaactgg taataaagat aagagataaa tctgactgta gacacaatga
      121 ctacgaatct ggctaataag ctctacaata gtcttgacaa tatgacattt atcatatcca
      181 agcccgatat cattacaacc aattaataaa aaaatatgtt taggctctaa atcagtaatt
      241 tggacagaaa aatggcgcaa taaccactga ctatctatcc cagcaatacc tcgattaata
      301 ataggcaaac atgaaccaaa agcttttttt aaaggaaaaa attcaatgag agaatcacct
      361 gcaaatacaa tgcctccttt tggggctaac tgatttttgt tacggtactc aatcaatttt
      421 tgttcttgat agtgacgtag ctcttctgaa acaatttcta acatctcagc ctccttagct
      481 tatctttatt gttagtataa caaaaagcct acttgacttc atcaaaagcg tttcactttg
      541 gttcagatga aactctagca gtaaaacgtt cgaagactta gtctaaaaga caagctcgaa
      601 ctttccgaat tgtgataaat taatacctac atcaatacct cacggacaaa acctgtatca
      661 gcttcattaa cgaccaaaaa gatagttgct cctgacaaaa ggcgcgtgtt acctgacaca
      721 acttgagatt tttgatggtg aatttgggtt gtaattaaga cattttctgg aagctttaac
      781 tctttaacat atttccctgc aatcttatca ccaacagtca attcaattaa tgttggttca
      841 acaagattag ttggatgctt cattgccatc ttatctagca tagcttcgta aattggctgt
      901 ccatttaaca aatccatcac taaataggag acaaaagtaa ctacagctat ggccattaag
      961 ggtttcaaat cacctaccat ttcagttacc aaaatcatgc ctgtcagtgg tgcttttgaa
     1021 attgccgcaa aataccctgc catccctaat atcaaaaaaa gagaaagaaa agattggttc
     1081 aataaaccta attgagaagc tatttgccca aataataggc ctgccaaagc acctaaagtt
     1141 aaaataggta aaaaaatacc tccaggtaag ccacttccat aactaaacat actaacaata
     1201 aatcgaataa taaaataaaa gaagatagtc atcaaaggca attgttgatt ggataaagaa
     1261 agtattaaac catgccctcc tccaagtaac tgtggtaaat aatagccaat cggtaaaatt
     1321 agaaaaacca taataatacc gtaaaaataa gctggcagat gtaaccaact ccctaaaata
     1381 acataaagtt tattaaaatt aaggataact atctcatata aatagcctaa gcagcctaga
     1441 aataatccaa gtaaaagcaa caaccaatat tgattgagtc ctaaaaaagg catcgcctct
     1501 gacattgcta agattggttt caatccaaaa atattcagag aaataaaatt agccactaaa
     1561 ctagccacta gcgctgtaat ccaaattaaa cgcgaaaaat gatggtaaat ttcttcaaca
     1621 acaaacaaga ggcctgctat tggagcatta aaagctgctg atagccctgc tgctgctccg
     1681 ctggcaatta gcactcgctt ttccaaacga ctcgatttta aaaacttagc taatcctttg
     1741 gctgacatag cacctaactg aatcgaagga ccctcacgac cgagcataaa tcccatagaa
     1801 atagccatga ttcctcctaa aaattttttc caaaggacac tccaccaatc tggggacatt
     1861 agtcccttta gttctccttc gacatggggg atccctgatc cttttatatc agaatcagac
     1921 tttactaaaa aacctacaaa aagaacagct aataaactga cacttagtat aggaagtagc
     1981 aacagcgaat tgatatgagc gtatcggtac cattcaatca cccagtcagc tgtcacttca
     2041 attaacaacc ggaataaact aacaataaca ccagcaataa taccaaccat aacaccacgc
     2101 caaacatagg caataattga tttgttggaa aaggtgaatt catttttatg gttttccatg
     2161 ttatcctctc tttcttttaa atacactgtt accattgtat caaagacaac cttaagttac
     2221 cagtgattct tttttagaac tgatttctgt aacccaaaaa acccactggg ctattggtta
     2281 ctcagtgggg tgatgaaaga aattactgtt acttcctttc ttacacaaat atttgactaa
     2341 taagccgaat tttctaaaac ctcttctccg tatcctccag cttttggcaa aggaagatta
     2401 gttgaattag gagctcttaa aatgcgaata ggtcttgcac ctgcctcttt ggcagcattt
     2461 atatcttcat cactatctcc gtaatgaatt tgtgagccgt ttttctttat ataatatgtt
     2521 ttatcatact gatacggctt cacagcttta tctcctgtat aattgatagc tattggcttg
     2581 tctaacttaa aatctttagc cagtgactta gctgttttat caatctcacc ttttttatac
     2641 atcgatcctc gagttcggcc agtaataaag acaattttat ctcctcgttt ttgatgcatt
     2701 gcgattagtt gctttgcata ctcttttgga atagaatcct gatctccccg tttagcgacc
     2761 aagtcccaaa atttttgctt atgcaaaaaa tcaaatgagc ctggagttat atattctttg
     2821 ccgtattgaa aatattggct agtaaataac aaagtatcat cgatatcaaa actaaccgtt
     2881 ataggttttt gtccttctaa actgctcgcg atatcagcaa tagaaatcaa ttttactgtt
     2941 gcctgatttg aaatggccgt tataccttct tgtgtataag gaactttagg gccactagct
     3001 tcgacaggca atgctatgat tccacaaaaa ctaactgtaa acaggatact ggtaaattct
     3061 tttttcatct aaaaacctcc ttaat
//
LOCUS       DAUELZ010000129         3048 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.130, whole genome shotgun sequence.
ACCESSION   DAUELZ010000129 DAUELZ010000000
VERSION     DAUELZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.130"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..224)
                     /locus_tag="VHI09_001562"
     CDS             complement(<1..224)
                     /locus_tag="VHI09_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ6121417.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLETIPNSGDGFPLKITINKELTGFKLAIT"
     gene            complement(214..1341)
                     /locus_tag="VHI09_001563"
     CDS             complement(214..1341)
                     /locus_tag="VHI09_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ6121418.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            complement(1338..2318)
                     /locus_tag="VHI09_001564"
     CDS             complement(1338..2318)
                     /locus_tag="VHI09_001564"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ6121419.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAADVLETALIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            complement(2458..3036)
                     /locus_tag="VHI09_001565"
     CDS             complement(2458..3036)
                     /locus_tag="VHI09_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEQ6121420.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLFQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFVNSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHITFKYAKSKVARCINTLKKFNDK"
BASE COUNT          936 a          538 c          579 g          995 t
ORIGIN      
        1 tcggtgatag ctaatttaaa acctgtgagt tctttattaa tagtgatttt taaagggaaa
       61 ccatcccctg aattaggtat tgtttcgagt aaacgtatta attggtagtg ccctacttta
      121 gtttgactaa atgtagtatc tcttagggta tattttttaa tgtttggact gattgtataa
      181 aggtatttac agtctttaag cttaatttct ttttcaaaag ccatctgttc ctcctaaaat
      241 attttttaat gtttgagcta attgattaac atcttcaatg ctattttgat ctgaaaaact
      301 gatacgaatt gattctttta aacgagaaga gtcatcccca tagtacgcag ctaaaacatg
      361 acttggatta acagctccag cagtacaagc agaaccagtt gatactgcaa tgcctgctaa
      421 gtcaagttgt gttagtaaaa tagtattttg gtagcctaaa aatccaatat tgagaacatg
      481 aggaagataa tgagtacctt gattaatata gtagggtagt ccttctagga ggctaatcaa
      541 gtgatgacgt aaactgataa tgtgatcagt tgattggtca agacaagtca tagcatcagt
      601 caaagcttgt gccataccaa taatacctaa catattttca gtacttgcgc gtcgctttcc
      661 ttcttgatca cccccgtgaa gaagagggtc gattggttgt ccgttactgt ataaaaagcc
      721 gcatccttta ggaccatgaa atttgtgagc agaagcagat aaaaagtcaa tcccaagttc
      781 gctaggaata atcttgagtt tgccaacagc ttgaacggca tcaacatgaa aagcagcttg
      841 atggtctttg agcagattcc caatgtcctt aataggtaac aggtcacctg tttcgttatt
      901 ggcatacatt atgcttacca aaatggtgtc atctctcaag gcttgtttta agtcagataa
      961 atttatttgt ccattttgac aaggcaaata agtgacctca aaaccaaatc gctcttcgag
     1021 ataggccatg gtatgaagaa cagagtggtg ctcaatagta gttgtgatga gatgtttgcc
     1081 ctttgcttga tgagctaaag catagccttt tatggccata ttattgcttt cagttccacc
     1141 ggacgttact ataatttgct gttcacttgc ccccaagttt ctagcgatag cttgtcggca
     1201 ttcacgaagg attttattgg ctcggcgccc gtagaaatga atactagagg ggttaccaaa
     1261 gttatcttgc atagctgctg tcatcgccct aatcacatta ggactaagtg gtgtggtggc
     1321 ggcgttatca aagtaagtca tgcgttattt ttgcctttag gttgataaga aaaaagtgga
     1381 cttaagggtc ttctttcatg gatacgaata atagcttctg cgattaaatc actggcgcta
     1441 agataagtaa cgttttctgg cacacgattt ttagttttaa cagaatctgt aacgataatt
     1501 tctttgatta gagctgtttc taatacatca gcagctccac cagcaaataa gccatggcta
     1561 gcgaccgcgt aagtatcagt agcgcctgag cgttcaagaa ttttagcagc ttctgcaaag
     1621 gttttcccag tatttaaaat atcatcaatt aaaatggctt ttttaccaga aacatcacca
     1681 ataatataac cttgttcacg ttcagaatca tcttgtgcat agtcgataat agcaatcggg
     1741 gaatcaagat attcagccaa gcttctagca cgtttaattc cagaattttt agggctaacg
     1801 acaacaacat cagaacctga taagcctaat ttactgtaac gttctgcaaa aagagggact
     1861 gtaaagaggt tatccactgg aatatcaaaa aaaccttgta cctgaacagc atgtaagtca
     1921 agcgtcacta cacgatcaat accagcttta gtcagcatat tagctactag tttagcagta
     1981 atcggttcgc ggggcttagc gacacggtct tgacgagagt aaccaaagta tggcaataca
     2041 atattgacgg tattagcact tgcacgttta caagcatcaa tcatgatgag taattcccaa
     2101 agattatcat tgacaggaaa actagtggat tgaataatat agatatcatc tccacgaact
     2161 gtttcttcaa tattgatcat aatttctcca tcggaaaatt gacgtgaaga catttttcca
     2221 agagggattc cagcagcttt agcaattttt tcagcaattg gaagatttga tgtgagtgag
     2281 aacagtttga tttgcttgtc agcatatcgt tcagtcatga tagatttagc tccttaactt
     2341 caataaaatt gggtaaactg ttttattaat gagtcacatt acccaaaagt gtttaattga
     2401 catccctatt ttaacagaaa aacagaaaaa tttcagtttt tctgtcgctt ttagccatta
     2461 tttatcgttg aactttttta aggtattaat acaccgagct actttgcttt tagcatattt
     2521 gaaagtaatg tgatgttcag ataaaaaaga atcaaaatca atctttcctt gaagagcgtc
     2581 acttacttct aattctaatt catagtcttt ggtattggca tataggttat agtctaaggc
     2641 caatttacct atcggtatca ctgtctcgcg acgaacggta gttaaattgc caaaagttac
     2701 cagtgcagat ggtttgatac cttttgaaat aataatatca agagcagttg attcaggaag
     2761 attcccatgt ttaatcatat cttttgcttg ttcaagaaaa agagggacat tgtactcacg
     2821 attccccact ttttctggca cttttaaggt taattcggcg ctatttacaa atgttcgaat
     2881 acgtaatgac attttgttag cttttaaatc aaatgctttt gtatcaatat agtaattagt
     2941 ttgtgtaaca ggcgtcacat gtttcatttg aaataaaaga cggttatact cattttttgt
     3001 aagtagagtt ttatattcga tttctaggtt agtcatggga aattcctt
//
LOCUS       DAUELZ010000130         3045 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.131, whole genome shotgun sequence.
ACCESSION   DAUELZ010000130 DAUELZ010000000
VERSION     DAUELZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.131"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(320..2857)
                     /locus_tag="VHI09_001566"
     CDS             complement(320..2857)
                     /locus_tag="VHI09_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEQ6121421.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQPTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
BASE COUNT          985 a          610 c          483 g          967 t
ORIGIN      
        1 agtaactatc acttcattta acttagtctc atttctgata acaatttcta acgagactat
       61 actacaaaaa gcttaaggtt tactaaaata ttaatactcc tttaaggcat ttaaagcata
      121 atgattaaat catttataat tgttcttatt caccacctgg taaaaaagta tttaagaggc
      181 taatagcctt cgctttaaag tgcaatctcc ttttgaatta aactatggat aatcatttag
      241 ttatagacgg aactaagaaa ggaagctagc tcactacaaa aaagagctgg aaagtccccc
      301 caactctttt gcttatggtc tagtaatgac tgagagctgc aataactgct tctttttctt
      361 tagtgatcaa agcaacacgt gcttcaattt cattaattcc catggtaata ttacggctaa
      421 tagccatatc atctagctta ggttcaaaga aagatttata ttctgctaga tgttttggtg
      481 ttttaaagtt acttgctgga taaataacaa atttatcaaa gctcatatct cctccaagtg
      541 cagatttaat ccaatcccaa ttttctctag cccattccca aatgatatct tgagtaaagc
      601 tttgtccaag caaagcattg taccaataag ataaatcctg aggtttaata atatctttat
      661 cttttaaact aacaagaata cgtctaatag tagttggttg ttttgtttga ctaaaagcaa
      721 ccgttaaatc actgcgtaaa ttattatcag tagttgctac ataagtctca aaataaatat
      781 caaccaagct atctgtttca aaatatttca tttggttggc taaaaccaag cgacgaacag
      841 ctgctggaat acttgcaata ttgttttgat atgcttcaaa aatagttctc aatccgtcaa
      901 taatcgtctg attttctgct agccacaagc ggcccaaggc aacctgacgt accatctcat
      961 cttcatctga ttcgtcagct tttttctcaa aaccatgctg attaaagtct tcttgataga
     1021 tggtggttac caagcgatta aaggattttt ctgctaagct tccttcatca atgaagcgtt
     1081 tcaaaccaga gaccacttgc tgaaccgctt ctgctaccat ataagactta gaatcgtcca
     1141 attgagcaat taagtcaacc aattcagcat aggaaatcaa gccactatct gccaataaac
     1201 gacgttcttg aataacttgt aaagcagaag tattatcaag ttgggcaagt tcagtgacta
     1261 aggcatctaa taatgatcct tggtaatctg taatgtaatg agctgtatta tcaatattga
     1321 aacgtagagc tccttcattt tctgctgcca attgactaaa gtttggaatg accatttctg
     1381 cctctgtcaa agtttcagga aggccatgcc aattagtgtt taaaggaatc ggccaaaggc
     1441 gacctttttc ttcacctttt ccaataaaaa attgtttttg actcaaaatc aattgatcgt
     1501 tttcaaggcg agctgttaaa actggataac ctggttgttc aagccaagca tccataaaag
     1561 cagctacgtc tttgcctgaa gtatctgata aaatattcca taaatcacgc ccgacagtat
     1621 ttcgatattg atatttttca aagtagtgat gtaagccaat ggcaaaatca cgatcaccaa
     1681 taaaacggcg taacatatgc atcaaacgac taccttttgc atagacaata gcaggatcaa
     1741 acaatgtatt tatttcatct ggatgattga cttcaacatg cactgattga acaccatcag
     1801 tagcatcacg ttttaaagca agaggaatac cgcctgtttg gaaatcttct attattttcc
     1861 aagacggctc aatagcctcg atagagacat attccatcat gttggcaaaa ctctcattta
     1921 accacagatc gtcccaccat ttcattgtaa ccaagtttcc aaaccattgg tgtgctatct
     1981 cgtgggcgat tacaagtgcc acttgttgtc ggctttgaac agtcgaattt tcatctacta
     2041 ataaataaat ttcacgataa gtgattaaac cccaattttc catagcacca gatgaaaaat
     2101 ctggaagcgc aatattaaga gattgcggga ttgggtaatg tactccaaaa tagtcttcat
     2161 aaaagttaat aacacgaact gcaatatcca atgaaaaatc aagcgagcta agaggatgcg
     2221 ctgttgtcgc atacacacca acagttgttc cttttttgct ttctacagtc tttccatgaa
     2281 gttctcctag cgcaaacgct agcaagtaag atgacattct tggtgtcgta tcaaatgtcc
     2341 aaagaccagt ctcctcgcga cggtcaatat tgacttctgg catattcgat aacgctatct
     2401 caccaatttc ttgatcaaag gtcaagctta aatcaaaagt agcttttgct tgtggttcat
     2461 cgatacatgg gaaagcttca cgtgcaaaat ggctttcaaa ctgagttgaa atgacttctt
     2521 ttttctcacc atttttagtg taatatgaag ggtaaatacc tgtcatatta tctgtaatgc
     2581 tacctgaaaa ttcaatgaca agcgtcatca ttcctgtttc aggtaattca acacgcacaa
     2641 cttcattgtc gttatccact tgataaataa cagcttcatt atcgagcaaa atagacttaa
     2701 tgtccaaatc tttttggtgg aaagccacat gattatctaa agcttcacca ttaatcgcaa
     2761 cattccccgt aaatgtttta gtctgacgat taatatctaa aaagatgtta taattttcag
     2821 gcacaaatgt ttcgatgaga tgttctactg tcttcatata actccttaat tctaataaca
     2881 aatcataata gatcaacaga tgatacctaa gtatcatctc tgtctacata tatttgttaa
     2941 cctaacaaaa tcagattcag caaagaaaac ttttcttgtt agttcatttt ctaacttgca
     3001 caatttcgta taaaaccagg gactggcaag ctgtttccca gtctc
//
LOCUS       DAUELZ010000131         2996 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.132, whole genome shotgun sequence.
ACCESSION   DAUELZ010000131 DAUELZ010000000
VERSION     DAUELZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.132"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..2253
                     /gene="cas9"
                     /locus_tag="VHI09_001567"
     CDS             <1..2253
                     /gene="cas9"
                     /locus_tag="VHI09_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEQ6121422.1"
                     /translation="TLTLFEDKEMIEERLKTYAHLFDDKVMKQLKRRHYTGWGRLSRK
                     LINGIRDKQSGKTILDFLKSDGFANRNFIQLIHDDSLTFKEAIQKAQVSGQGHSLHEQ
                     IANLAGSPAIKKGILQSVKVVDELVKVMGHKPENIVIEMARENQTTQKGQKNSRERMK
                     RIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDH
                     IVPQSFIKDDSIDNKVLTRSDKNRGKSDDVPSEEVVKKMKNYWRQLLNAKLITQRKFD
                     NLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVI
                     TLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYK
                     VYDVRKMIAKSEQEIGKATAKRFFYSNIMNFFKTEITLANGEIRKRPLIETNEETGEI
                     VWDKGRDFATVRKVLSMPQVNIVKKTEVQTGALTNESIYARGSFDKLISRKHRFESSK
                     YGGFGSPTVTYSVLVVAKSKVQDGKVKKIKTGKELIGITLLDKLVFEKNPLKFIEDKG
                     YGNVQIDKCIKLPKYSLFEFENGTRRMLASVMANNNSRGDLQKANEMFLPAKLVTLLY
                     HAHKIESSKELEHEAYILDHYNDLYQLLSYIERFASLYVDVEKNISKVKELFSNIESY
                     SISEICSSVINLLTLTASGAPADFKFLGTTIPRKRYGSPQSILSSTLIHQSITGLYET
                     RIDLSQLGSD"
     gene            2253..>2996
                     /gene="cas1"
                     /locus_tag="VHI09_001568"
     CDS             2253..>2996
                     /gene="cas1"
                     /locus_tag="VHI09_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ6121423.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLE
                     TTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLWKQMSWPEAVK
                     SEVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTL
                     FGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFELKHANQFNQFNFASDI
                     MEPFRPLVDKIVYENRNQPFPKIKRELFTF"
BASE COUNT         1023 a          466 c          595 g          912 t
ORIGIN      
        1 acattgactt tatttgaaga taaggaaatg attgaggaac gacttaaaac atatgctcac
       61 ctctttgatg ataaggtgat gaaacaactt aaacgtcgcc attatactgg ctgggggcgt
      121 ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggtaaaac aatattagat
      181 tttttgaaat cagatggttt tgccaatcgc aattttatac agctgattca tgatgatagt
      241 ttaacattca aggaagctat tcaaaaagca caggtgtctg gacaaggcca tagtttacat
      301 gaacagattg ctaacttagc tggcagtcct gctattaaaa aaggtatttt acagtctgta
      361 aaagttgttg atgaactggt caaagtaatg gggcataagc cagaaaatat cgttattgaa
      421 atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa
      481 cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa
      541 aatactcaat tgcaaaatga aaagctctat ctctattatc tacaaaatgg aagagacatg
      601 tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga tcacattgtt
      661 ccacaaagtt tcattaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa
      721 aatcgtggta aatctgatga cgttccaagt gaagaagtag tcaaaaagat gaaaaactat
      781 tggagacaac ttctaaacgc taagttaatc actcaacgta agtttgataa tttaacgaaa
      841 gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt
      901 gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa
      961 tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta
     1021 gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat
     1081 catgcccatg atgcgtatct gaatgccgtc gttggaactg ctttgattaa gaaatatcca
     1141 aaactagaat cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt
     1201 gctaagtctg agcaggaaat aggcaaagca accgcaaaac gtttctttta ttctaatatc
     1261 atgaactttt tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta
     1321 atcgaaacta atgaggaaac cggagaaatt gtctgggata aagggcgaga ttttgccaca
     1381 gtgcgcaaag tattgtccat gccacaagtc aatattgtca agaaaacaga agtacagaca
     1441 ggtgcactga ctaatgaatc aatttatgct agaggaagtt ttgataaatt aatcagtcgt
     1501 aaacatcgtt ttgagtcctc taaatatgga ggatttggca gcccgactgt tacttatagt
     1561 gtgttagtag ttgcgaaatc caaagttcaa gatggaaaag taaaaaaaat aaaaactgga
     1621 aaagaattaa ttgggattac attattagat aaactagttt ttgaaaaaaa tccattaaaa
     1681 tttattgaag ataagggata cggaaatgtt caaattgata agtgcattaa gttacctaaa
     1741 tatagcctct ttgaatttga aaatggcact cgaagaatgc ttgctagtgt tatggcgaat
     1801 aataatagca ggggagattt gcaaaaagca aatgaaatgt ttttaccggc gaagttggta
     1861 acactgctat accatgcaca caaaattgag agttctaaag aattggagca tgaagcatac
     1921 attttggacc actataatga tttatatcaa ttgctgagct atatagaacg atttgcttca
     1981 ttatacgtgg atgttgaaaa gaacatttct aaagtaaaag aacttttcag caacattgaa
     2041 agctattcta tttctgaaat ttgttcctcc gttattaatt tattaacact tacagcatcc
     2101 ggtgctcctg cagattttaa attcttaggc acaacaattc cacgaaaacg ttatggttct
     2161 ccccagtcta ttctcagttc tacccttatc catcaatcca tcactggtct ctatgagacc
     2221 cgtattgatt tgagtcagct aggaagtgac tgatggctgg ttggcgtact gttgtggtaa
     2281 atacccactc gaaattatcc tataagaata atcatctgat ttttaaggat gcctataaaa
     2341 cggagctgat ccatttatca gaaattgata ttttgttatt agaaacgacc gatattgtct
     2401 tgtccactat gctggtaaaa cggctagtgg atgagaatgt ccttgtcata ttctgtgatg
     2461 ataaacgatt accaacagct atgctgatgc ctttttatgg tcgtcatgat tcgagtctac
     2521 agctttggaa acaaatgtcc tggccagaag cagtcaaatc ggaggtttgg acgacgatta
     2581 ttgctcaaaa gattttgaat caatcttgct atctaggagc atgctcctat tttgaaaaat
     2641 cccaatctat tatggattta tatcatggtt tggaaaattt tgatccgagt aatcgagaag
     2701 ggcatgcagc gagaatttat tttaatacac tttttgggaa cgatttctca agagatttgg
     2761 agcatccaat caatgcaggt ctggattatg gttatacttt attattgagt atgtttgcgc
     2821 gtgaagtggt tgtgtctgga tgtatgactc agtttgagct taaacacgct aatcagttta
     2881 atcagttcaa ttttgctagc gatattatgg aaccatttag gcctttagtg gataagattg
     2941 tttatgaaaa tcgaaatcag ccttttccca aaataaagag agagttattt actttc
//
LOCUS       DAUELZ010000132         2880 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.133, whole genome shotgun sequence.
ACCESSION   DAUELZ010000132 DAUELZ010000000
VERSION     DAUELZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.133"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            15..419
                     /locus_tag="VHI09_001569"
     CDS             15..419
                     /locus_tag="VHI09_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEQ6121424.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDIDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            421..699
                     /locus_tag="VHI09_001570"
     CDS             421..699
                     /locus_tag="VHI09_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEQ6121425.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLDS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSTYQVVKKAKAIEKGFFSLGN"
     gene            689..1477
                     /locus_tag="VHI09_001571"
     CDS             689..1477
                     /locus_tag="VHI09_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEQ6121426.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVTLEDQ"
     gene            1480..2790
                     /locus_tag="VHI09_001572"
     CDS             1480..2790
                     /locus_tag="VHI09_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEQ6121427.1"
                     /translation="MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALVMLKSGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLTKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKSDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
BASE COUNT          939 a          500 c          594 g          847 t
ORIGIN      
        1 gatagaggaa aaatatggtt accttatttt tatcacctag ctgtaccagt tgtcgtaaag
       61 caagggcatg gttagtaaaa cacgaagttg actttcaaga gcataatatt attacaagtc
      121 cattaagccg tgatgaatta atgtcaatcc tctcttttac agagaatgga acggaggata
      181 ttatttcaac acgttcaaag gtttttcaaa aacttgatat tgatgtagaa gagctttcaa
      241 tttcagattt aattgattta attgctaaaa atccaagtct tcttcgtcgc cctattatca
      301 tggatcaaaa acgcatgcaa attggtttta atgaagatga aattagagcg tttttatcga
      361 gagattatcg caagcaagaa ctacgtcaag caacgataaa agcagaaatt gaggggtgat
      421 atgtcaggta attattatta tccacttgac ttaagttgga gcactgaaga aatatcctca
      481 gtgcttcatt ttttgaataa agtggagtta gcatatgaaa aaaaagtaga tgcaaaacaa
      541 ctactagata gttataagac ttataaaacc attgtcaaaa gtaaagctca ggaaaagcaa
      601 atcgaccgtg attttcaaaa agttagtggt tattcgacct atcaagttgt taaaaaagcg
      661 aaggcaattg agaaaggatt cttttccctt ggaaactaaa tacgcatttg caaggcaaat
      721 cattaaagaa gcagggcttt ttatcaagtc aaaaatgtca gagcagctag atattcaggt
      781 gaaaacacaa tttgatgatc tggttaccaa tgttgatcaa gaaactcaac agttactgat
      841 ggatcgtatt catcagacct atccttgtga tgccatttta gctgaagaaa atgacgttcg
      901 tcaccccatt aatcaaggaa atgtttgggt catcgatccc attgatggaa cggttaactt
      961 tattgtgcag ggatcacagt ttgcagtgat gattgcctat tatgaacaag gaattggtca
     1021 atttgggctt atttatgatg tgatggcaga tcaattacta gctggtggtg gtgattttga
     1081 agttacttta aacggtgata agcttccagc ttaccaagag aaacctttag agcgtagctt
     1141 aattggctgc aatgcaggca tgtttgccag aaatgatcgt aaccttgctc atctaattgc
     1201 aaagacttta ggtgtaaggg tttatggtgg tgcaggtatc tgcatggtta aggtcatgaa
     1261 gcaggaatta ctggcctatt tttcatttat ccaaccatgg gattacgcag cagcaaaagt
     1321 actaggcgat aaactgggtt atgttttact aactattgac ggatatgagc ctgattttca
     1381 aacacgtcaa aaaatcatgt ttgttcctaa atgccagctc actaggatag cgtcttttct
     1441 aacaaaggac tctgccgtca cacttgaaga tcaatagaaa tgatgtcatt accaaaagaa
     1501 tttattaaca cttatcaagc aatactaggc aaagaattag aagatttctt ggcaagtttt
     1561 aaccaagagc cggtcaatgc ttttcggatt aaccccttaa agaatcaact aaaaaccttt
     1621 gaacacccca ttccaaatac cttatgggga tattatggta aactatctgg caagtctcca
     1681 gagcatgtgt cggggttggt ttattcccaa gaaccagcgg ctcaaatggt ggcacaagta
     1741 gcggctcctc aaaaggggag tcgtgtttta gacttggcag ctgcaccagg aggaaaatca
     1801 actcatttat tagcttattt agacaataca ggtcttttag tcagcaatga aatttctaag
     1861 aagcgcagca aggttttagt tgaaaatatc gaacgttttg gcgctagaaa tgttgtggtg
     1921 actaatgaat ccgcagatcg tttagccaag gtattttctc attattttga tactatcgtc
     1981 tttgatggac catgttctgg tgaaggaatg tttcgcaagg accctgatgc tattcaatat
     2041 tggcatcacg ggtatcccgc agaatgcgcc aagttgcaaa aatctatttt agaagacgcc
     2101 ttagtcatgt taaaatcagg aggagaattg atatactcca cctgtacttg ggctcctgaa
     2161 gaaaatgaag atgttgtgca gtggctctta gagacttata cctttttaga attggtcgat
     2221 gttccaaaac taaatggaat ggttagtggt atcggacttc cagagactgc tagaatgtac
     2281 cctcatcgtt atcaagggga agggcaattt gtggccaagc taaaagataa gcgtcaagaa
     2341 ggacaaagca ctaaactaaa agcacctaaa tccaacttga ccaaagacca gttacgtctt
     2401 tggaaaatgt ttgaaaaaga ccatctaaac cttaccctat caggaacact acaaaccttt
     2461 ggtgattacc tatacctctt gccagatggt cttcctgctt tagatggcct taaaattgct
     2521 cggaacggtt tagaattagg cgtcttcaag aagaaacgct ttgaaccctc ttacgccttg
     2581 ggattagctt taaagtctga tgaggtccga tcatctattg agcttagaga ggaagacttt
     2641 agaccttatg tgtcaggtaa cgtcattact ttgccccaat cttatccaac aggctggtac
     2701 caattactca ttaatggcaa tggtctagga tttgccaagg tcgttgacaa gaccatcaaa
     2761 aataatttcc ctaaagggtt aagattttag cgtaaaaaat aagattaaaa tgttaaatat
     2821 ttaaagaaaa aatgctttca aagcataggt agatatgtta ctataaagaa tatgtggcac
//
LOCUS       DAUELZ010000133         2822 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.134, whole genome shotgun sequence.
ACCESSION   DAUELZ010000133 DAUELZ010000000
VERSION     DAUELZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.134"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            2..1981
                     /locus_tag="VHI09_001573"
     CDS             2..1981
                     /locus_tag="VHI09_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEQ6121428.1"
                     /translation="MLTSFTLLVTDVQADQVDVQFLGVNDFHGALDNTGTAYTPSGKI
                     PNAGTAAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANSALLQDEPTVKVFN
                     KMKFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEHEASHQTIVIANVI
                     DKKTNDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQNYEHYQFLDVAET
                     IAKYAKELQEQHVHAIVVLAHIPATSKDGVVDHEMATVMEKVNQIYPEHSIDIIFAGH
                     NHQYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFIKTPSANVVAVAPGIKTENS
                     DIKAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNLVTTAQLTIAKKTF
                     PTVDFAMTNNGGIRSDLVVKNDRTITWEAAQAVQPFGNILQVIQMTGQHIYDVLNQQY
                     DENQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLTTTYTVVVNDFLYG
                     GGDGFSAFKKAKLIGAINTDTEAFITYITNLEASGKTVNATIKGVKNYVTSNLESSTK
                     VNSAGKHSIIIISKVFRNRDGNIVSSEIISDLLTSTENTNNSFGKKEITTNKNTISNS
                     TLPITGDNYKMSPIMTILALISLGGLNAFIKKRKS"
     gene            complement(2063..2473)
                     /locus_tag="VHI09_001574"
     CDS             complement(2063..2473)
                     /locus_tag="VHI09_001574"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ6121429.1"
                     /translation="MIREIITDHFLLKQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLNLTGLTAQICQHELDHLEGILI"
BASE COUNT          958 a          531 c          461 g          872 t
ORIGIN      
        1 tatcctgact agttttactc tattagttac agatgttcaa gcagatcaag ttgatgtgca
       61 attccttggc gtcaatgatt ttcacggcgc tcttgataat accggaacag cttacacacc
      121 aagtggtaaa ataccaaatg ctgggacggc tgctcaatta ggtgcttata tggatgacgc
      181 tgagatagat ttcaagcaag caaatcaaga cggaacaagt atacgtgttc aagctggaga
      241 tatggtcgga gccagtcctg ctaactctgc acttttacaa gatgagccta ctgtgaaagt
      301 ctttaacaaa atgaaatttg aatatggcac tcttggtaat catgaatttg acgaaggact
      361 agatgaattt aaccgtatca tgacaggtca agcgcctgat cctgaatcaa caattaatga
      421 tatcaccaaa caatatgagc acgaagcttc gcatcaaacc atcgtcattg ctaatgtcat
      481 tgataaaaaa actaatgata tcccctatgg ttggaaacct tatgctataa aagacatagc
      541 cattaatgac aaaatcgtta agattggctt cattggtgtt gtgactacag agattccaaa
      601 tctcgtttta aagcaaaact atgaacacta tcaattttta gatgtagctg aaaccattgc
      661 caaatatgct aaagaactgc aagaacaaca tgttcatgct attgtggttt tagctcatat
      721 tcctgcaaca agtaaagatg gtgttgttga tcatgaaatg gctacggtta tggaaaaagt
      781 gaaccaaatc tatcccgaac atagtattga tattattttt gcaggacata atcatcaata
      841 cactaatgga actatcggta aaacacgtat cgttcaagcc ctctctcaag gaaaagctta
      901 tgcagatgtc cgtggtacgc tagatactga taccaatgat tttattaaaa ctccatcagc
      961 aaatgttgtt gctgtagcac caggtatcaa aacagaaaat tcagatatca aagctataat
     1021 aaatcatgct aatgatattg ttaaaacagt tactgaacga aaaatcggaa ctgcaactaa
     1081 ttcttcaact atttctaaaa cagaaaatat tgataaagaa tctcctgtcg gtaacttagt
     1141 aacaacggct cagcttacta ttgctaagaa aacttttcca actgttgact ttgctatgac
     1201 caataatggt ggtattcgaa gtgacctagt tgtcaaaaat gaccggacca tcacctggga
     1261 agctgcacag gctgtacaac catttggtaa tatccttcaa gtcattcaaa tgactggtca
     1321 acacatttac gatgtcctaa atcagcaata cgatgaaaac cagacctatt ttcttcaaat
     1381 gtcaggttta acatacactt atacagataa tgatcctaag aactctgata cccccttcaa
     1441 gatagttaag gtttataaag acaatggtga agaaattaac ttaacaacta cttacaccgt
     1501 tgttgtcaac gactttcttt atggtggtgg tgatggcttt tcagcattta aaaaagctaa
     1561 attaatcgga gctattaaca cagatactga agctttcatc acatatatca caaatttaga
     1621 agcatcaggt aaaactgtta atgctactat aaaaggggtt aaaaattatg taacttcaaa
     1681 ccttgaaagt tcgacaaaag ttaatagtgc tggtaaacac agtatcatta tcattagtaa
     1741 ggtttttaga aatcgtgatg gcaatatagt gtctagtgaa atcatttcag accttttgac
     1801 ttctactgaa aacactaata acagctttgg caaaaaagag ataacaacaa acaaaaatac
     1861 tatctctaat tccactcttc caataacagg ggacaattat aaaatgtctc ctattatgac
     1921 aatccttgcc ttgataagct taggtgggct aaacgctttc attaaaaaaa gaaaatccta
     1981 gcgtttacta gtaattgatc atccttatat aatcaggcat tttcgccaaa aaaacctaaa
     2041 gtgtcctact ctaggttttt tgttaaatta aaataccttc tagatgatct aattcgtgct
     2101 ggcatatttg agcagttagg cctgttaagt ttaaacgctt tggtcgccaa ttatggtcca
     2161 aatactcaac agtaatctta tcataccgct gtgttttgcg atagccagat aatgacaaac
     2221 aagattcttt agtttggtaa attccctttt tagaaaccat cacaggatta aacatcacca
     2281 aatcaataaa tcccatactc acaataacaa tgcgtttttg ttcaccaatc atattggcag
     2341 ccatcccaag acattcctgc ctataaaagg ctaaggtatc ttgtaaatct tggccaatcc
     2401 atagatcttc tttcttggcg acttgtgctt tttgctttaa taaaaaatga tctgttatga
     2461 tttctctaat cattcttatc tccttaaact ttttttaatt cataaactac atgttcaatg
     2521 gactattatc accatatgat agtcttagta tcatctgttt aaaacatcag atgattcctt
     2581 atttcaagat atcttgggca actttatgaa attctacaat atgagtacca aataaaaatt
     2641 gacctgcatc agcagattgt tgctaagttc tcatcacaat attatcgagt ttactgtctg
     2701 cttcttcaag tgtctaagag agatagttat tgctttgtga gattttcaaa ccagatactg
     2761 ctttctcttg catttgcagc cttaacaggt ccaaataaaa cacctgctgt ttgaaatgca
     2821 tt
//
LOCUS       DAUELZ010000134         2732 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.135, whole genome shotgun sequence.
ACCESSION   DAUELZ010000134 DAUELZ010000000
VERSION     DAUELZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.135"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..472
                     /locus_tag="VHI09_001575"
     CDS             <1..472
                     /locus_tag="VHI09_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEQ6121430.1"
                     /translation="QEASLQIFNIKDYNLDNLLENPNKFDDEKYWITVDTYSAQGERA
                     TAFSNTLNNITSKYWRVVFDTKGDRYSSPVVPELQILGYPLPNADTIMKTVTTAKELS
                     QQKDKFSQKMLDELKIKEMALETSLNSKIFDVTAINANAGVLKDCIEKRQLLKK"
     gene            736..1620
                     /locus_tag="VHI09_001576"
     CDS             736..1620
                     /locus_tag="VHI09_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ6121431.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIART
                     IAYFKAFEADLAGIAIGSFGPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     PHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMAQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            1715..2668
                     /gene="manA"
                     /locus_tag="VHI09_001577"
     CDS             1715..2668
                     /gene="manA"
                     /locus_tag="VHI09_001577"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEQ6121432.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATMVLDNMVATTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
BASE COUNT          839 a          474 c          611 g          808 t
ORIGIN      
        1 tcaggaagca agtctacaaa tttttaatat caaagattat aatctagata atttgttgga
       61 aaatcccaat aaatttgacg atgaaaaata ttggattact gtagatactt acagtgcaca
      121 aggagagaga gctactgcat tcagtaatac attaaataat attactagta aatattggcg
      181 agttgtcttt gatactaaag gagatagata tagttcgcca gtagtccctg aactccaaat
      241 tttaggttat ccgttaccta acgccgacac tatcatgaaa acagtaacta ctgctaaaga
      301 gttatctcaa caaaaagata agttttctca aaagatgctt gatgagttaa aaataaaaga
      361 gatggcttta gaaacttctt tgaacagtaa gatttttgat gtaactgcta ttaatgctaa
      421 tgctggagtt ttgaaagatt gtattgagaa aaggcagctg ctaaaaaaat aaacaaagta
      481 actttcttag atagcaacat tcagattaaa ttaacaaaat gtgactatga taaaggtttg
      541 ctggaattga ttaaccaaaa gactaaaaat ctgagatgaa tagtcccaga tttttagtct
      601 tttataggtt ttgatgacat aaagctaaat aatcgttaga ctaccagaaa gtggcgcttg
      661 tccttgagac atggctgtct tgataagatc ctgtgtcatc ataaagataa aaaagaacaa
      721 gaaaaaggag aaaacatggg aaaattatat ggaagcattg aagctggtgg gacaaagttt
      781 gtctgtgccg tgggtgatga ggagtttaca gtggtggata aaacacaatt tccgaccacc
      841 acaccagaag aaaccatcgc gcgaaccatc gcttatttta aagcttttga ggcagatttg
      901 gctggtattg ctattggttc ttttggacca attgatattg atccgtcgtc agagacttac
      961 ggttacatca caaccactcc taagtcaggc tgggctaatg tggatttgtt aggacaactt
     1021 tcggctgctt ttaagattcc ttttgatgtg acaacagatg ttaatagctc tgcttatggt
     1081 gaggtgcttg ctcgtcctgg tgtggaaagt ttggtctatt acaccatagg tactggtatt
     1141 ggggcgggag ctattcaaca tggccacttt attgggggtt taggtcatac agaagcgggt
     1201 catacctatg tcatgcccca tcctgatgat atggccaagg gatttttagg tgtttgccct
     1261 tttcataaag ggtgtctgga agggatggca gcaggtccaa gtattgaggc cagaacggga
     1321 gtgagaggtg aacggcttga tcaggaagct gatgtttggg acattcaagc cttctacatt
     1381 gcccaagcag cgctgcaagc aactatgctt taccgtcctc aagtgattgt ttttggcgga
     1441 ggtgtcatgg cgcaagaaca tatggtgttg cgcgtgcatg ataaattcac taccctttta
     1501 agcggttatt tgccagtccc tgatttgaca gactacattg ttactccagc tgttgctgat
     1561 aatggctccg ctactctggg aaattttgct ttggccaagt tggctgcaca aggaaaatag
     1621 gatctgtttt tgcctgcttg tctagtaaaa gaaagcgcag tcagctagaa catgctataa
     1681 taaacagaga ttaacatcag aagcgaggaa tattatgtca gaaccattat ttctaaaatc
     1741 aactatgcac gacaggattt ggggtggcac caagttgaga gatgtctttg cttacaacat
     1801 cccaagtgac accacaggtg agtattgggc tatttctgct catcctaatg gtgtttcgac
     1861 agttactaat ggacgttatc aaggacaacc tttaaacaca ttatatgcgc aagagccggc
     1921 cttgtttggg aatccaaagg aagaagtttt tccactgtta actaagattt tagatgctaa
     1981 tgactggcta agtgtccaag ttcatcctga tgatgcttat ggacgtgaac atgaaggaga
     2041 attaggcaag acagaatgct ggtatattat ttctgccgaa gagggttctg aaattgtata
     2101 cggccatcag gccaagtcca aagaagacct tcgtgccatg attgaagcag gcgcttggga
     2161 tgatttgctg actcgtgtcc cagtcaaagc aggcgatttt ttctatgttc caagtggcac
     2221 catgcatgcg atcggcaagg gaattcttat tttagaaacc cagcagtcat ctgataccac
     2281 ttatcgggtt tatgattttg accgtaagga cgttaatggt aatcttcgtg accttcatat
     2341 tgaaaagtct attgatgtct taacgattgg taagccagaa aatagcgtgc cagccactat
     2401 ggttttggat aatatggtag caacaacttt agtctcaaca ccatttttca ctgtttacaa
     2461 atgggtaact agtcaaatgg ttgacatgaa acaagctgcg ccttatcttt tagtgagtgt
     2521 tttaaaaggt caaggaaaac tctatgtgga ccagaaggct tatgagctag aaaaaggcat
     2581 gcactttatc ttgcctaacg atgttaaatc gtggtcattt gatggtcaat tagaaatgat
     2641 tgtgagtcac cccaatcaac tcgtttaata ccatggtatc aatatgagaa aaaaggataa
     2701 aattatgact ctctgtttct ctgataactt ta
//
LOCUS       DAUELZ010000135         2720 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.136, whole genome shotgun sequence.
ACCESSION   DAUELZ010000135 DAUELZ010000000
VERSION     DAUELZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.136"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            31..1578
                     /gene="sfbI"
                     /locus_tag="VHI09_001578"
     CDS             31..1578
                     /gene="sfbI"
                     /locus_tag="VHI09_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein SfbI"
                     /protein_id="HEQ6121433.1"
                     /translation="MVSSYMFVRGEKMNNKMFLNKEAGFLAHTKRKRRFAVTLVGVFF
                     MLLAYAGAIGFGQVAYAADEKTVPNFKSPDPDYPWYGYDAYGKGYPGYDISKYYHDLR
                     VNLNGSQVYQVYCFNIQKIFPYNVKSVTQKWFKKVEGNSDTFGLYAMNPRVQGEELSQ
                     KLRSVMYNAYPKNANNIMDGLDTLNAIKVTQSAVWYYSDKSEFEVDKQWESELNNHEI
                     DQEQVTLMREALRKLISSNLEETVEKKLPENYKLNIFNPQDTSIQHLLSAEFVPENPP
                     RPGETPEYGPKTPELDGSPIPEDPKHPDDNLEPTLPPVMLDGQEVPEVPSESLEPALP
                     PLMPELDGQEVPEKPSIDLPIEVPRYEFNNKDQSPLAGESGETEYITEVYGNQQNPVD
                     IDKKLPNETGFSGNMVETEDTKEPEVLMGGQSESVEFTKDTQTGMSGFSETATVVEDT
                     RPKLVFHFDNNEPEVEENREKPTKNITPILPATGDIENVLAFLGILILSVLSIFSLLK
                     NKQNNKV"
     gene            2376..2516
                     /locus_tag="VHI09_001579"
                     /pseudo
     CDS             2376..2516
                     /locus_tag="VHI09_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          960 a          459 c          580 g          721 t
ORIGIN      
        1 aatcaaaaaa atatctttct tgacaataac atggtaagct catatatgtt tgtgagagga
       61 gagaaaatga ataacaaaat gtttttgaac aaagaagccg gttttttggc acacacaaaa
      121 agaaaaaggc gatttgctgt cactttagtg ggagtctttt ttatgctttt ggcatacgcg
      181 ggtgctatcg gttttggtca agtagcctat gctgcggatg agaagactgt gccgaatttt
      241 aaaagcccag atccagatta tccctggtat ggttatgatg cttatggtaa ggggtatcct
      301 ggatatgata tcagtaagta ttatcatgat ttaagggtaa atctaaacgg aagtcaggtc
      361 taccaagttt attgctttaa tattcaaaaa atttttccgt acaatgtgaa aagcgttact
      421 caaaaatggt ttaaaaaagt tgaggggaat tctgatactt ttggacttta tgcaatgaat
      481 ccaagagtcc aaggagaaga acttagtcaa aaacttcgaa gtgtcatgta taatgcttat
      541 cctaagaatg ctaataatat tatggacgga ttggacactt tgaatgccat aaaagtaacc
      601 caaagtgcag tgtggtatta ttctgataag tcagagtttg aggtagataa acagtgggag
      661 tctgaattaa ataatcatga aattgatcaa gagcaagtaa ctttgatgcg tgaagctcta
      721 agaaaattaa tttcttctaa tctagaagaa acagtagaaa aaaaattgcc tgaaaactat
      781 aaactgaata tttttaatcc acaggatact tcaattcaac accttttaag tgctgagttt
      841 gtaccagaaa atccccctag acctggagaa acccccgaat acggacctaa aactcccgag
      901 ttggatggta gtcctattcc cgaagaccca aaacatccag atgataattt agaacctaca
      961 ttgccgccag tgatgctaga tggtcaagaa gtcccagaag ttccaagcga gagcttagaa
     1021 cctgcgcttc ccccattaat gccagaacta gatggtcaag aagtccctga aaaacctagt
     1081 attgacttac ctattgaagt tcctcgttat gagtttaaca ataaagacca atcgcctcta
     1141 gcgggtgagt ctggtgagac ggagtatatt accgaagtct atggaaatca acagaaccct
     1201 gttgatattg ataaaaaact tccgaatgaa acaggttttt caggaaatat ggttgagaca
     1261 gaagatacga aagagccaga agtgttgatg ggaggtcaaa gtgagtctgt tgaatttacg
     1321 aaagacactc aaacaggcat gagcggtttc agtgaaacag cgactgttgt tgaagatacg
     1381 cgtccgaagt tagtgttcca ttttgacaat aatgagcccg aagtggaaga gaatcgggaa
     1441 aaacctacaa aaaatataac acctatcctt cctgcaacag gagatattga gaatgttttg
     1501 gcctttcttg gaatccttat tttgtcagta ctttctattt ttagcctttt aaaaaacaaa
     1561 caaaacaata aagtctgacg taaaagtatc ccataagaaa tagtgcgcaa atatgctgaa
     1621 tagtggataa ctgctacagt caagacaagc gttaattcaa aaagtactac ctatacacct
     1681 gataataata aggtgacaga taattcagag caagtaggat ggctaaaaaa tggtgtattc
     1741 tatgtataat agacttacaa gaaatcaagt tattgttaaa cgtcgtaaag cttaacgctt
     1801 tcaaaagtcg aaatcaatcg agactgtctt tatgcagtct cgatttttaa gtacgacaga
     1861 catgttgtac gtctgaggtg aaaatcctcc gtggacaccc gctaaaggtg aatccaaaag
     1921 cgatgcccaa gtctgactat cgtgagtagt ggggagagga agggagagtg aaatcgtggc
     1981 tctacaaaca gaaacgtgat atcaaggcgt atatagcgga tgagtctaaa gtccaaaaaa
     2041 gtagtagtaa cctataggcg taaatatgag agtaaacgag aaaagatgta caccttatcc
     2101 tttgaggtct catgagcgtt ctagacgtag taacaacgaa tgatgagaag tcagccgatg
     2161 ccatagtagc gatgaaactt ctgtaatgga agtagagcga agggctaaac aattaaccga
     2221 atatgcctac ttcacttgtg tatgtaaaac tagcggtctg atagaactgg atgcggtcac
     2281 gtattgacta gaggaaagtt cactaatata agatgtccct cagacaccaa aataagaaag
     2341 gaatacgcaa agaggagtgg aaaagtcgtc cacaatcaag atggagcgac cattgtcaat
     2401 tagtagctta aaattcagtt ctaaaacaag ctatatcaaa aacagtcctc gcaaaacggg
     2461 gactgttttc ttgcttggat gactacttag aacgacatgc gttaaaagtt cattgaaccg
     2521 ccgtgtgccg aacggcacgc acggtggtgt gagaggggct agaaattagc ccctactcga
     2581 tttctatcta agctaaaaat agaaatcaaa ttaaaagact agaaaataaa tttcagagtg
     2641 cttggaaata tctccaaata aacatttttc tcaaaaatga ggttttcatg taagaaatat
     2701 ggagaaaaaa atgagcttac
//
LOCUS       DAUELZ010000136         2703 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.137, whole genome shotgun sequence.
ACCESSION   DAUELZ010000136 DAUELZ010000000
VERSION     DAUELZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.137"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            68..610
                     /gene="hpt"
                     /locus_tag="VHI09_001580"
     CDS             68..610
                     /gene="hpt"
                     /locus_tag="VHI09_001580"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEQ6121434.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
     gene            632..2611
                     /gene="ftsH"
                     /locus_tag="VHI09_001581"
     CDS             632..2611
                     /gene="ftsH"
                     /locus_tag="VHI09_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEQ6121435.1"
                     /translation="MKNNKNNGFVKNSFIYILMIIVVITGFQFYLKGTSTQSQQISYS
                     KLIKHLKAGDIKSLSYQPSGSIIEVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSL
                     VLPSDTILKEMTAAADKNGTELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGG
                     GGARGAMSFGKNKAKSQSKGDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFV
                     GADLENVLNEAALVAARRNKIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGR
                     VAEEIVFNAQTSGASNDFEQATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEK
                     AYSAQTAQMIDDEVRELLNQARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIY
                     ETGKMPVDLETDDNEAHALSYDEIKNKMTESESAE"
BASE COUNT          921 a          394 c          567 g          821 t
ORIGIN      
        1 aaagcttgta aacatgatat aatgttggct aaactgtata ttgataaatt agaaaaaggg
       61 tgacagcatg ctcgagcaag atattcaaaa aattctttac tctgaaaatg atattattcg
      121 gaaaaccaaa aaattaggag aacaattaac aaaagattat caagaaaaaa atccgcttat
      181 gattggtgta ttaaaaggat cagttccttt tatggcagaa ctaatgaaac acatcgatac
      241 tcatgttgaa attgatttta tggtagtatc aagttatcac ggcggtacat ctagcagtgg
      301 tgaagttaaa attttaaaag atgtagatac caatattgaa ggaagagata tcataattgt
      361 tgaagatatt attgacaccg gtcgcacctt aaaatattta cgagatatgt ttaagtaccg
      421 taaagctaat actatcaaaa ttgcaacact gtttgataaa cctgaaggtc gtgttgttaa
      481 aattgaagca gattatgttt gttacaatat tccaaatgaa tttatcgtag gttttggttt
      541 agattacgca gaaaattatc gcaatcttcc ttacgtaggt gtattaaaag aagaagtcta
      601 ttcaaaatag aaaaaggtca atgtttactg tatgaaaaat aataaaaata atggttttgt
      661 taaaaattct tttatatata tattgatgat tattgttgtc atcacaggat ttcaatttta
      721 tcttaaggga accagcacac aaagccaaca gattagttat tccaaattaa ttaaacatct
      781 taaggctgga gatataaaat ctttaagtta tcagcctagc ggtagtatta ttgaagttaa
      841 agggaaatat gagaaacctc aaaaagtgac agttaattca ggactatcat ttttaggtgg
      901 aagagcttca acacaggtaa cagagttctc atctttagta ttacctagtg ataccatctt
      961 gaaagaaatg acagcagcag ctgataaaaa tggaacagag cttacagtta agcaggaaag
     1021 ttcaagtggt acttggatta ccttcttaat gagcttttta cctattgtta tttttgctgc
     1081 tttcatgatg atgatgatga atcaaggtgg tggaggagct cgtggtgcta tgagtttcgg
     1141 caaaaataag gcaaaatctc aatcaaaagg tgatgttaaa gtgagattta cagacgttgc
     1201 tggtgccgaa gaagaaaaac aagaattagt agaagttgtt gattttttga aaaatcctaa
     1261 aaaatataaa tcactaggag cacggattcc agcgggagtt cttctagagg ggcctccagg
     1321 tactggtaaa acattactag ctaaagctgt agcaggtgaa gctggagtgc catttttcag
     1381 tatttcaggt tcagattttg tcgaaatgtt tgtcggtgta ggtgctagtc gtgttcgttc
     1441 tttatttgag gatgctaaaa aagctgagcg tgcgatcatc tttatcgatg aaattgacgc
     1501 tgtaggtcgt cgtcgtggtg ctggcatggg aggcggtaac gatgaacgag aacaaaccct
     1561 taaccaatta ctcattgaaa tggatggttt tgagggtaat gagaacatta tcgttattgc
     1621 tgcaactaac cgtagtgatg ttttagatcc agctttacta agaccaggtc gttttgatcg
     1681 taaggtttta gttggtcgcc ctgatgttaa aggacgtgaa gccattttac gtgttcatgc
     1741 taaaaataaa ccacttgcaa atgacgttaa tttgaaagta gtagcccaac agactccagg
     1801 ttttgtaggt gctgatcttg aaaatgtttt aaatgaagct gcgttagttg ctgctcgcag
     1861 aaataaaatt aaaattgatg ctagtgatat tgatgaagct gaagatcgtg ttattgctgg
     1921 accatcaaag aaagatcgca ctatttcgca aaaagagcga gaaatggttg cttatcacga
     1981 agctggtcat actattgttg gtttagtttt atctaatgcg cgtgtagttc acaaggtaac
     2041 tattgtacct cgtggacgag caggtggtta tatgatcgct cttccaaaag aagatcaaat
     2101 gcttctttct aaagaagatc tgaaagagca attagcagga ttgatgggtg gtcgtgttgc
     2161 tgaagaaatt gtttttaatg ctcaaacatc aggtgcgtca aatgactttg agcaagctac
     2221 tcagatagca agggccatgg ttacagaata tggtatgagt gaaaagcttg gaccagttca
     2281 atatgaaggt aaccatgcga tgatgccagg acagatctct cctgaaaaag cttactctgc
     2341 ccaaacagct caaatgattg atgatgaggt tcgtgaactc ttaaatcaag ctcgtaatca
     2401 agcagctgat atcatcaatg agaatcgaga tactcataaa ttgattgctg aagctcttct
     2461 caaatatgaa actctagacg ctgcacaaat taaatcaatt tatgaaactg gtaaaatgcc
     2521 agttgattta gaaactgatg ataatgaagc tcatgcgtta tcttatgacg aaattaaaaa
     2581 taaaatgaca gaatctgaat ctgcagaatg atataaaaaa gctgaactta tagattaagt
     2641 tcagcttttt tatcaacaca atatccttgt aaacgttatg aattgtaata ggtgcctaat
     2701 tgc
//
LOCUS       DAUELZ010000137         2680 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.138, whole genome shotgun sequence.
ACCESSION   DAUELZ010000137 DAUELZ010000000
VERSION     DAUELZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.138"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            54..1037
                     /locus_tag="VHI09_001582"
     CDS             54..1037
                     /locus_tag="VHI09_001582"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEQ6121436.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            complement(1196..2566)
                     /locus_tag="VHI09_001583"
     CDS             complement(1196..2566)
                     /locus_tag="VHI09_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEQ6121437.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDAKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIELFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDVSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
BASE COUNT          780 a          554 c          505 g          841 t
ORIGIN      
        1 aaaataagac cgtatgagct tttgctctaa taaaattaag gagatgttta gaaatgactg
       61 caactaaaca acacaaaaaa gtaatcttgg tcggtgatgg tgccgtcggt tcatcatacg
      121 cttttgcatt ggtaacacaa aatattgctc aagaacttgg tattattgat atttttaaag
      181 aaaaaactca aggagatgca gaagatttaa gccacgctct agcttttaca tcacctaaaa
      241 aaatctatgc agctgactac tcagactgtc atgatgctga tctcgttgtc ttaacagctg
      301 gtgctcctca aaaaccaggt gaaacacgcc ttgaccttgt tgaaaaaaac cttcgtatca
      361 ataaagaggt tgttacacaa atcgttgcat ctggtttcaa aggaatcttt cttgttgctg
      421 ctaacccagt tgacgttttg acttactcaa catggaaatt ctcaggtttc cctaaagagc
      481 gtgttattgg atcaggtaca tcactagatt cagcccgttt ccgtcaagca ctagctgcta
      541 aaattggggt tgatgcgcgt tcggttcacg cttacatcat gggagaacac ggtgactctg
      601 aatttgctgt ttggtcacac gctaacgttg ctggtgtagg tctttacgac tggctccaag
      661 ctaatcgtga cattgatgag caaggcctag ttgatctttt catttctgta cgtgatgctg
      721 cttactcaat catcaataaa aaaggtgcta ctttctacgg tattgcagtt gccctagctc
      781 gtattacaaa agctattctt gatgatgaaa atgctgttct tccgctttct gttttccaag
      841 aaggccaata cgaaggtgtt gaagattgct atattggtca accagctatc gttggagcgt
      901 atggaattgt tcgtccagta aacattccac ttaacgatgc tgaattgcaa aaaatgcaag
      961 cctctgcaaa tcaattgaaa gctatcattg atgaagcctt tgctaaggaa gaatttgctt
     1021 ctgctgctaa aaactaatca caaaagagac ttcggtctct ttttgttttg cctgatattc
     1081 cttttccaac gtgctataag aggataatca taagtttaga acttttcagg ttgcaacaat
     1141 taaaaagcaa aaaagccagt gctgctgaac tgctctggct ttttcctatc agatactagt
     1201 ctttggcacc aagtgctgcc attgtgatgt agttgtaagg tttgttgaaa tgtggtaaga
     1261 agaaaatatc agttaatgcc aacttttcaa tggttacgcc ttcttggata gcaagtgaga
     1321 acatgtgaat tcccattgac acatcttcac gggctgccat ttgagcaccc aagatacgac
     1381 gtgagtcttt atcgtaaaca atcttaatgg ttactgggaa gttaccgtgt tcgataaatt
     1441 caggtttttg gttatcagta tactcagtca ctgcagcatc aaaaccaagt cgttttgctt
     1501 tttcaagtgt caaaccagtt gataccatgt gcaagccgta aatggaaata ccgtttgagc
     1561 cttggacacc gattccttca aggtctgtac cacaagcgtt atgtgctgct acaattcctg
     1621 tacggacggc atttgaagct aaagcaatgt agtttgtatc gcgagtagca ttatcgtaga
     1681 tagttgcaca gtcaccgata gcataaacac ctggaataga agtttcttgg cgtttattga
     1741 caaggaaagc accattacgg aaaagctcaa tcttaccatt tccaagagtt gtgtttggac
     1801 ggaaaccaac ggcgaggata accatatcca catcgtactc atttttgtca gtaatgatct
     1861 tttcaacctt accattacca gctacttctt taactgtttc accaaaggct agttgaatac
     1921 catgttcttc catgttttta gccattaagt cagtcaaatc acggtcgtaa taacctgcca
     1981 agcaagtgtc cacgacatca atcagaacca cttctttacc tttacgttgg aaagcttcag
     2041 ctaactcaac accaatgtaa ccagcaccaa ctacggctac acgtttaatg tctttgtttt
     2101 caagctttgc aatcacatca gctgagtttt ggtataactt cacaaattga agattttcaa
     2161 gagttgcttc aaattcaagt gatccttctt tgatttcggc acctttaatc ggtggtaaga
     2221 taggttgtga gccagttgca aaaatcaact tgtcgtaagt ttccacgtgg tttttaccat
     2281 caacaagcgc tgtcactgtt ttggcatcgt agtcgattga ttgaacaggt gattccatgt
     2341 aaactttagc ccctaaggac tctaattctt ctttatctga atagaaaagt ccttctggtc
     2401 cagcaatttg ctcaccaatc caaagtgcca taccacagcc taaaaatgaa atatttgagt
     2461 tttggtcaaa tacaacaatc tcattagcat caccgtagtt tgttaacata gtcttgatac
     2521 aggccgtacc agcatggttt gcaccaacaa caacgatttt actcatatat tttgttcctc
     2581 atttcacaat attatatagg gctctattat accacagtca aaaaacgctt ccaaatatat
     2641 tgctcacttt tttgagttat tttttcttta gtcaactgtt
//
LOCUS       DAUELZ010000138         2670 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.139, whole genome shotgun sequence.
ACCESSION   DAUELZ010000138 DAUELZ010000000
VERSION     DAUELZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.139"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(12..2171)
                     /gene="nrdE"
                     /locus_tag="VHI09_001584"
     CDS             complement(12..2171)
                     /gene="nrdE"
                     /locus_tag="VHI09_001584"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ6121438.1"
                     /translation="MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYHPEFITELASIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKTNPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYADGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(2191..2409)
                     /locus_tag="VHI09_001585"
     CDS             complement(2191..2409)
                     /locus_tag="VHI09_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEQ6121439.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIETDNLVFSGFQPAKLKELI"
BASE COUNT          860 a          452 c          517 g          841 t
ORIGIN      
        1 ttcttaacca attaaatgac acaagattca cattggtttg cgcccacttc ttccccatca
       61 tccgtaaagg tacggatata gtagatagat ttaatgcctt tattgaaagc gtagtttcgt
      121 aagatggata aatcacgagt ggtttgtttg ctttgtgttt tccactcata aagctccata
      181 ggcaactcac tacgaaggaa tagagttaat gacaagcctt ggtccacatg ttcggtcgca
      241 gcggcataga catcaataac tttgcgcatg tccatatcgt aagcagatgt atagtaagga
      301 atggtatccg tagacaaacc atttgcagga tagtagattt taccaatttt cttttcttga
      361 cgctcttcga tgcgttgtgt gattgggtga atagaagcag agcagtcatt gatataagaa
      421 atagagccat ttggagcaac tgctagtcgg ttttgatgat aaagaccatc tttttgaacg
      481 gcgtcgcgaa gagcctccca atctgaagct tgcggaataa aatggtcttt aaagagactt
      541 ttgaccaaat cagattttgg aacaaagtgt cctgtaacgt atttatcaaa gtaactacca
      601 tcagcgtact tagagttctc aaagccaaca aaggtagttt ggcgctcacg agcgatgtta
      661 ttggattcga ccaaggtcca ataattcagg agcataaagt aaatatcagt aaactcgatg
      721 gattctggac tgccatattc aatatgatgt tgagcaaggt aagaatgtag tcccatagct
      781 ccaaggccaa aagtatgagc ttggctattg ccatgtttaa tggttggaac agcttcaatg
      841 cttgatgaat cagtaacaaa agttagggca cgtgtcatgg tcttaataga acggccaaag
      901 tctggtgagg tcatcatgtt caggatatta gtggaaccta agttacatga aatatcagtt
      961 cccatttcta caaactcttg cgcatcattg ataaggctag gtgtttgaac ttgtaaaatt
     1021 tcagaacaca agttgctcat gatgattttt ccatcgatag gattagtttt attagctgta
     1081 tcaatattga tgatataagg gtaaccagat tcttgttgta attttgaaat ctctgtttca
     1141 agatcacgag ctttaatttt agtcttagta attttagggt tcgccactaa ctcatcgtac
     1201 atattggtaa tgtcgagata gttaaaggga atgccatatt ctttttcaac attgtaagga
     1261 ctaaagagat acatgtcctc gtttttacga gctaattcgt agaatttatc aggaacggta
     1321 atccctagtg acaaggtttt aacacgcacc ttttcatcgg cattttcttt tttagtagat
     1381 aagaaagcaa tgatatcagg atgaaaaaca tttaggtaaa caacaccagc tccttgacgt
     1441 tgcccaagtt gatttgaata agaaaaacta tcttcaaata atttcataac aggaacaact
     1501 cctgaggctg caccagcata gcctttgatt ggtgcgccag cttcacggag gttagacaag
     1561 gtaatcccaa cacctccacc aatacgggat aattgcaagg cagagttaat ggaacgtccg
     1621 atagaattca tgtcatcagt tacttgaatc aagaaacaag agaccaattc accacgacgg
     1681 cttcgaccag catttaaaaa ggaaggagta gccggttggt aacgttggtt aatcatttca
     1741 acggctaaat cttttgctaa gtcttcttga ccatctgcaa aatacaaagc attaaacaag
     1801 acacggtctt caaggttttc taaataatgc tctccatcat ttgtttttaa ggcgtattgc
     1861 tggtagaact tgtaggctgc cataaatgat ttaaagcgaa aattttctga tttgattatg
     1921 ctagctaatt cagtaataaa ttcagggtgg tatttctgaa taaaagctga ttcaatgtaa
     1981 tcatttgaga ttaaatactc aattttttcc gtaatggaag taaaagacat ggtgtttggc
     2041 agcacatttt cagcgaaaaa agcttttaaa gcttctttgt ctttatgaag tggaatttta
     2101 ccattaacag gacggttaat ttcattattt aggcgaaaat atgaaatatc gccaagatct
     2161 ttgagactca tattattttt ccttgtctta ttaaatcaat tcttttaact ttgcaggttg
     2221 gaagccagaa aatacgaggt tatctgtttc gataacagga gcagatgtga agccaagact
     2281 tttcacataa tccacttttt caggatgttc atcgatatta atttcctgga agttggctcc
     2341 gtgttgttct aaaaatttct tagtcatttt gcactgcatg caattatttt tggaatatac
     2401 tgtaatcatg tcttgtcctt ctaaaattag taatggttcc atcttaaatg gaattactat
     2461 aatagcatat aaaaaataaa gctaaaaagc aagctataaa cttatctaaa acacaacata
     2521 ttgtgttgaa ataaaaaaca gaaacctaaa tattgtgtca atctggattc ttatggttag
     2581 agctctagcg ttttggtgat agaagctaag cagttttaat aaagtattaa agtttgaaaa
     2641 ataaattgtg aaaaacgtga aaataaaaga
//
LOCUS       DAUELZ010000139         2632 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.140, whole genome shotgun sequence.
ACCESSION   DAUELZ010000139 DAUELZ010000000
VERSION     DAUELZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.140"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            131..937
                     /locus_tag="VHI09_001586"
     CDS             131..937
                     /locus_tag="VHI09_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEQ6121440.1"
                     /translation="MFLIISRNSLMTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATYLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLK"
     gene            1170..2525
                     /gene="dnaA"
                     /locus_tag="VHI09_001587"
     CDS             1170..2525
                     /gene="dnaA"
                     /locus_tag="VHI09_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEQ6121441.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNPKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQTQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
BASE COUNT          957 a          456 c          424 g          795 t
ORIGIN      
        1 agaggaacta caaagaaaaa agcagatatc aaattaacaa aaactactca agatttgact
       61 aaaacgcagt aaacaagcta cctaaatact attacatttt atcacataaa acagtccaaa
      121 ggactgtttt atgtttctta ttatatccag gaactccctt atgacaaaag aattattgat
      181 agacctacca attgaagata ttgttacaaa tccctatcag ccaaggatac aatttaatca
      241 aagagaactc caagacctag caacttctat taaatcaaac ggcctcattc aacctattat
      301 tgttcgaaaa tctgacattt ttggctatga gttagttgct ggagaacgac gacttaaagc
      361 ttcaaaaatg gctggtctaa aaaaagttcc agctatcatc aagaagatct ctacactcga
      421 gagcatgcaa caagctatag ttgaaaattt acaacgttct aaccttaacg ctatcgaaga
      481 agctaaagcc tatcagttat tggttgaaaa aaaacacatg actcacgatg agattgctaa
      541 atatatggga aaatcaagac cttatattag caatacctta cgtctgttac aactcccagc
      601 acctatcatt aaagcaattg aagaaggaaa aattagtgcg ggacacgcgc gtgctctttt
      661 aactttgagt gatgataagc aacaactgta cctcactcat aaaatacaaa atgaaggcct
      721 aagtgttagg caaattgagc aactggttac ttctactcca agttcgaagc tatctaaaaa
      781 aactaaaaat atatttgcca cttatttaga gaaacaattg gctaaatcat tgggactctc
      841 tgtcaatatg aagctgacag caaaccatag tgggtacctt cagatatctt tttccaatga
      901 tgatgaatta aacagaatta tcaacaagct aaagtagctt gttgatattc ttttttttct
      961 tttttagttt tccacatgaa aaatagttga aaacaatagc ggtgtcacct taaaatgtct
     1021 tttccacagg ttgtggagaa ctcaagttaa cagtgttaat ttattttcca cagattgtgg
     1081 aaaaactaac tattatccat tgttctgtgg aaaactagaa tagtttatgg tagaatagtt
     1141 ctagaattat ccacaagaag gaacctagta tgactgaaaa tgaacaaatt ttttggaaca
     1201 gggtcttgga attagctcag agtcaattaa aacaggcaac ttatgaattt tttgttcatg
     1261 atgcccgtct attaaaggtc gataagcata ttgcaactat ttacttagat caaatgaaag
     1321 aactcttttg ggaaaaaaat cttaaagatg ttattcttac tgctggtttt gaagtttata
     1381 acgctcaaat ttctgttgac tatgttttcg aagaagacct aatgattgag caaaatcaga
     1441 ccaaaatcaa tcaaaaacct aagcagcaag ccttaaattc tttgcctact gttacttcag
     1501 atttaaaccc gaaatatagt tttgaaaact ttattcaagg agatgaaaat cgttgggctg
     1561 ttgctgcttc aatagcagta gctaatactc ctggaactac ctataatcct ttgtttattt
     1621 ggggtggccc tgggcttgga aaaacccatt tattaaatgc tattggtaat tctgtactat
     1681 tagaaaatcc aaatgctcga attaaatata tcacagctga aaactttatt aatgagtttg
     1741 ttatccatat tcgccttgat accatggatg aattgaaaga aaaatttcgt aatttagatt
     1801 tactccttat tgatgatatc caatctttag ctaaaaaaac gctctctgga acacaagaag
     1861 agttctttaa tacttttaat gcacttcata ataataacaa acaaattgtc ctaacaagcg
     1921 accgtacacc agatcatctc aatgatttag aagatcgatt agttactcgt tttaaatggg
     1981 gattaacagt caatatcaca cctcctgatt ttgaaacacg agtggctatt ttgacaaata
     2041 aaattcaaga atataacttt atttttcctc aagataccat tgagtatttg gctggtcaat
     2101 ttgattctaa tgtcagagat ttagaaggtg ccttaaaaga tattagtctg gttgctaatt
     2161 tcaaacaaat tgacacgatt actgttgaca ttgctgccga agctattcgc gccagaaagc
     2221 aagatggacc taaaatgaca gttattccca tcgaagaaat tcaaacgcaa gttggaaaat
     2281 tttacggtgt taccgtcaaa gaaattaaag ctactaaacg aacacaaaat attgttttag
     2341 caagacaagt agctatgttt ttagcacgtg aaatgacaga taacagtctt cctaaaattg
     2401 gaaaagaatt tggtggcaga gaccattcaa cagtactcca tgcctataat aaaatcaaaa
     2461 acatgatcag ccaggacgaa agccttagga tcgaaattga aaccataaaa aacaaaatta
     2521 aataacatgt ggaaaagaat atcttttatg aaatagttat ccacatgttg tgaacaacca
     2581 tttagtcttg cattctctcg tttatttaga gttatccact atatacacaa ga
//
LOCUS       DAUELZ010000140         2605 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.141, whole genome shotgun sequence.
ACCESSION   DAUELZ010000140 DAUELZ010000000
VERSION     DAUELZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.141"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..323
                     /locus_tag="VHI09_001588"
     CDS             <1..323
                     /locus_tag="VHI09_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121442.1"
                     /translation="FELDGNGIPSTFRQENIDPLVNKWWYDNVSTEIIPNLIKKLKEN
                     PLFNIGFLVDDILERMYKENIPKSYLTSVPLVISKKARSTLSIKSNGSQKINKSKFAQ
                     ILSL"
     gene            389..1141
                     /locus_tag="VHI09_001589"
     CDS             389..1141
                     /locus_tag="VHI09_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ6121443.1"
                     /translation="MAIPDLDKFSSSLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIREAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            1138..1740
                     /locus_tag="VHI09_001590"
     CDS             1138..1740
                     /locus_tag="VHI09_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121444.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(2216..2497)
                     /locus_tag="VHI09_001591"
     CDS             complement(2216..2497)
                     /locus_tag="VHI09_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ6121445.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGT
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRSALRIERFKKTYDVQTYR"
     gene            complement(2504..2554)
                     /locus_tag="VHI09_001592"
                     /pseudo
     CDS             complement(2504..2554)
                     /locus_tag="VHI09_001592"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT          847 a          534 c          418 g          806 t
ORIGIN      
        1 tatttgaatt agacggaaat ggcataccct caacatttag gcaagaaaac attgatcccc
       61 tcgtcaataa atggtggtat gataatgttt cgacagaaat tatccctaac ctaataaaaa
      121 aactcaaaga aaatccacta tttaatatcg gattcctggt tgatgatatt ctagaacgaa
      181 tgtataaaga aaatatccca aaatcttacc ttacgtccgt cccattagtt atttccaaaa
      241 aagcaaggtc aactttatcg attaagtcaa acggaagtca aaaaataaat aaatcaaaat
      301 ttgcacagat tttgtccctt tagagacgga tgacttacac attcaacgtg gattattctt
      361 cccttggaga aaggggattt cagaacgctt ggcgattcct gatttagata aattttcatc
      421 tagcttacaa caggggaaaa ttcctgtcat aaagcacttc gaactcaatt tagataaggg
      481 gagtcactgg gctattgctg gaaattcagg ttctgggaaa ccgtacgctc tcacttactt
      541 cttgagtgtc ctgaaaccta tgtccggcct aattatcatt gacccaaaat tcgatacccc
      601 tagccaatgg gcaagagaaa ataaaattgc cgttatccac ccagtagaaa accattcaaa
      661 atctgatttc gtttcacaag tcaatgaaca attaaaccag tgtgcaactc tcattcagaa
      721 acgacaagct atcttgtatg ataatcccaa tcatcaattt acccatctaa ctattgttat
      781 tgatgaagtc ctcgctctat cagagggagt caataagaat atcagagaag cttttttctc
      841 cctactatca caaattgcct tgttgggaca tgctactaaa atccatctat tcttgggaag
      901 ccagcgtttt gaccataaca ctattcccat ttcagtgagg gaacagctaa acgtattatt
      961 gcaaattgga aatatcaatc agaaaaccac tcaattctta tttccagacc tagatccaga
     1021 ggggattgtc attccaacag gacacggaac aggtatcatt caagttgtcg ataatgaaca
     1081 ctcctatcaa gttctaccct tactctgtcc aacctactat actaagcgag gtatcctatg
     1141 accactaaaa cctacttcat gagaagcttc aatctcatat tatacttact gcttattgct
     1201 ccaatctgct atgtgttcgg ttggctgttc aatcttgttt cagttcaatt atctaccgtc
     1261 acatttgtca atctggatac ggttaaaaac gttactgata ccattagttc tgtatgttat
     1321 ggattcgctc ttatttcttt acttctagcc ttattatctg gattaaagat gatacaacgc
     1381 ttgaatacag atagccttgg gaactactgc caatctattt atcgcacctt tctattaaga
     1441 cacttccttt tccagcgtga acgagtacag aaggtaagca actttgaaca ccagaccgtt
     1501 accactatca atccaatcca taacgggttt aaccgagctg tacgtaagtg tatcgtagat
     1561 atacgaaaag atgccattac tgtatttcta aaagttccta gagaccaaca ggggcaaaaa
     1621 atcctaaagg atacgggagt acaactgaaa gaagagattg tcatccaaca cacagactat
     1681 tatttctctt ctcctgttcg agttcgtaac caattgtggt ttattggtca aaagcgttaa
     1741 ttccttgggg ctgtggcttg cgttaagcaa aagccagaca gccccttttt tatattctta
     1801 tctatcatga cctattgggg ttactacgac aacgaaagtt agaagctgtg ggtatttatt
     1861 tatcttgaaa agattgctga actaacgcaa taactactct accattcact ctacctttta
     1921 ttaagcaact atcaataaat attgatacaa taaggtttat aacagctaaa atagaaaata
     1981 cttaaccttt acagaagcaa aataattgag atgagaaaag cctatgaaat gaacgtttca
     2041 aaggcttttt tcgtgtttaa aaaaacaaat gaaaaaacaa aatagagagt agggttatta
     2101 acactgtcaa tcctgtgtac gcaataacaa tccatgcttt ttgataagga tattttgctt
     2161 tttctttaat cttatcaatc tgatctaagc tctcaccctt tgctatagct tcaagttatc
     2221 ggtaagtctg aacatcatat gtctttttaa atcgctcaat tcttaaggcg ctacgcatga
     2281 ttatcaacca taagatagca accggaccat atccccacca acctaaccaa gacattatag
     2341 gataaatact gatagcaatt aggagtgtcc caaataacat aatatgcgta ttcctatcac
     2401 attgtttaat ctcctcatcc ttaataatta aagtcatttt tgggatatct tctgctatta
     2461 gttgctctaa aggaacctgt aaggacttac ttaacatcaa aagattttgc aggtctggat
     2521 agcttttacc gttttcccag tttgagatag tcttcttgaa acatacatca tttcagcaag
     2581 ctcttcttgt gacatctgta atttt
//
LOCUS       DAUELZ010000141         2587 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.142, whole genome shotgun sequence.
ACCESSION   DAUELZ010000141 DAUELZ010000000
VERSION     DAUELZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.142"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            22..804
                     /locus_tag="VHI09_001593"
     CDS             22..804
                     /locus_tag="VHI09_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ6121446.1"
                     /translation="MLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAVSPDYAPFEFK
                     ALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKADLAISGISHTK
                     ERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLSGKKVAAQKGSIEEGLVKIQLPK
                     ANLISLTAMGEAINELKAGQVYAVTLETPVAAGFLAQHKDLALAPFSLKTSDGDAKAV
                     ALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
     gene            945..1121
                     /locus_tag="VHI09_001594"
     CDS             945..1121
                     /locus_tag="VHI09_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEQ6121447.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(1249..1611)
                     /gene="mscL"
                     /locus_tag="VHI09_001595"
     CDS             complement(1249..1611)
                     /gene="mscL"
                     /locus_tag="VHI09_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEQ6121448.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAA
                     APTQEELLTEIRDLLAQK"
     gene            1783..2587
                     /locus_tag="VHI09_001596"
                     /pseudo
     CDS             1783..2587
                     /locus_tag="VHI09_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          884 a          431 c          493 g          779 t
ORIGIN      
        1 aaaaaaataa gatttggttt tttgctttgc ctcctacttt atcctttagc agcctgtaac
       61 aagtctgaac aacttaatca ctacgaaaga attaaaaaaa caagaaaatt agtcgttgct
      121 gtaagccctg attatgctcc ttttgaattt aaagctttag ttaatggaaa agatactatt
      181 gttggagcag atgttcaatt agctcaggct attgctgatg aattagacgt tgatcttgag
      241 ctttcaccaa tgagttttga taatgttttg tctagtttac agactggtaa agctgatctt
      301 gctatttcag gaatctcaca tactaaagaa agagctaaag tatatgattt ttcaattcct
      361 tactatcagg cagaaaacgc tatagtgatg agagcatctg atgctaaagt gaccaaaaat
      421 attagtgacc taagcggtaa gaaagtagcc gctcaaaaag gtagtatcga ggaaggttta
      481 gttaaaatac aattaccaaa ggccaatctg atttctttga ctgctatggg agaagccatt
      541 aatgaactaa aagcaggaca agtctatgca gttaccttag aaacacctgt agctgctggt
      601 tttttagccc aacataagga tctggcttta gcacccttta gcttaaaaac cagtgatgga
      661 gatgccaaag cagtggccct tcctaaaaat agtggtgact taaccaaagc cgttaataag
      721 gttattgcta agttagatga gcaagaacgg tacaagtcat ttattgcaga gacaatagca
      781 ctaacgaaaa atactatgaa gtaataaggt taataaaaac tttattagta tccgttggaa
      841 aagtgtttgg aataaacaat aaaagaggtt tgactttttt taaaaaataa tgtaatataa
      901 ttgggaacgt gaaaatgtta caatatcttg aggaggtgaa acacatgtca aaaacagtag
      961 tacgtaagaa tgaatctctt gacgatgctc ttcgtcgctt caaacgttct gttactaaag
     1021 ctggtactct tcaagaatca cgtaaacgtg aattctacga aaaaccttct gtaaaacgta
     1081 aacgtaaatc agaagcagct cgcaaacgta aaaaattcta atggtaaaaa ataaaaacag
     1141 ttctcacaat tagaactgtt tttatttttt atccagaaga agcaagggag cttttttgat
     1201 atcaaaaaaa gcagttagac atgcctaact actttatcaa aaatcgcctt atttttgagc
     1261 caacaaatca cggatttctg tcaataattc ttcttgagta ggagctgcag cttctttttc
     1321 ttttttaggc atagcttttt cagcggcttt tacaacaaag aaaagactgg taccgatgat
     1381 aagaaaatta ataacggctc ctaaaaagct accatattta acaccattcc atgacaattg
     1441 agtgatattt tcaacatttg cagctttcag agctgggttt aaaataagcg gtgtgataat
     1501 atcgttcaca aaagaagtga caatagcacc aaaagctccc ccgatgataa ctgctacagc
     1561 aagttcaata atgtttcctc taaacaaaaa tgcttttaat tctttaacca ttaaatattc
     1621 ccccttttaa tatctctaag ttaagcatag cataattagt aaacaaaatc caatggaatc
     1681 acttcagcac gccaaacgca tgaagaaact tttgttttca gaaaactaga ttcataatga
     1741 caaaatcaat caaagtagta cactatagat gaggtgacta cgatgattga ttttattatt
     1801 tctattgatg attgcgcagt tgaattggat agtcgtcaat cttggaaaat tcgctccccc
     1861 ttatcaacca ttctatttct tgtcttcgtt tgtcagttag ctagcattga aacctggaag
     1921 gagatggaag attttattga aatgaatgaa ccattgtttg cgacctacgt tgatttgagt
     1981 gaaggttgtc cgtctcatga taccttagag cgtgtgatta gtcttgttaa ttcagaccgt
     2041 ttaaaagagc ttaaagttca atttgagcaa tcattgacaa gcttagatgc cgttcatcaa
     2101 ctgatttcag tggacggtaa aacgattcga ggcaatcgag gtaaaaatca gaagcctgtt
     2161 catattgtaa cggcttatga tgggggtcat catcttagtt tgggacaggt agcggttgag
     2221 gagaaaagta atgaaattgt tgccattcct cagttattgc ggacattgat atccgtaaaa
     2281 gcattgtaac gatagacgca atgggcacgc agacggctat cgttgatacg attattaaag
     2341 gtaaagcaga ctattgctta gccgtcaaag gaaatcaaga aacactttat gatgatattg
     2401 ctctttattt tagtgatgtc aacttattgg aagaactcca agaaaatgca cagtattatc
     2461 agactgttga aaaatctagg ggacagattg aagttagaga atactgggtg tcttccgata
     2521 tcaaatggtt gtgtcaaaac catcccaaat ggcataagtt acgtggtatt gggatgactc
     2581 gtaacac
//
LOCUS       DAUELZ010000142         2408 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.143, whole genome shotgun sequence.
ACCESSION   DAUELZ010000142 DAUELZ010000000
VERSION     DAUELZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.143"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            37..594
                     /locus_tag="VHI09_001597"
     CDS             37..594
                     /locus_tag="VHI09_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ6121449.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRVPVGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            728..2272
                     /locus_tag="VHI09_001598"
     CDS             728..2272
                     /locus_tag="VHI09_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ6121450.1"
                     /translation="MSILEVKQLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGYLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DFSKLT"
BASE COUNT          780 a          355 c          488 g          785 t
ORIGIN      
        1 taactaaatc gtttaaaaag ttgttggagg aaaaaaatgt catctcaaaa attagctaat
       61 tatattgaat tgagtatgta tgccgcttta atttttattt cagtacaagt gttgcgtgtc
      121 cctgtcggta tgcagtttat tcatcttggc aatgcattag ttgttattgc agtcttaatc
      181 tatggtagta aaaaaggagc tctagttgct agtcttggtt tagggatctt tgatgttttg
      241 aacggctatg cttcagtcgt ttggataact atcttagaat ccttggctat ttgtttaatt
      301 ttgcatttgg tatatgaaag agctatgcaa tctagtaaaa aaccaaaagc cattataaca
      361 ataacagtta ttgctgcatt gtcaaaatta actattaacc ttataaaata cttactgtta
      421 gcttatatag gtactcagct accactttcg gtatcttttt gggtagctct tggaaaaatt
      481 ggtggtagtt tcggaacatc ttttgtgact ttagttgctg ttcctctttt gtatcctgtt
      541 atcaaaaact taaaagaaaa ggttacaatc ggacaagcaa aaagaggttt ttgatacaag
      601 taccttatcg ataaaaaagc aaggtacact tttagtcaaa tattgacaaa aggttacaat
      661 tttgccataa tgaaataact ataaagccgt tttgacggct tttttgatga cagttaagga
      721 agaaacgatg agtattttag aagttaagca gttgagtcac ggttttgggg atcgggctat
      781 ttttgaaaat gtgtcatttc gcctcttaaa aggtgaacat attggactag ttggggcaaa
      841 tggtgaagga aaatcaacct ttatgagtat agtcacagga catttacagc ctgacgaagg
      901 aaaagtagag tggtcgaagt atgtcactgc aggttacctg gatcaacata cagtgttgga
      961 atcaggacaa accgttcgtg atgtcttgcg aactgctttt gatgagttat ttaagaccga
     1021 gaatcgtatt aatgagattt acgcgtcaat ggcagatgat aaagctgata ttgctgtttt
     1081 gatggaagaa gtaggtgagc ttcaagatcg tttagaaagt cgtgatttct atactttgga
     1141 tgctaagatt gatgaagtag cgcgtgcgct tggtgttatg gattttggaa tggagtcaga
     1201 tgttacatcc ttatcaggtg ggcaacgaac aaaggtttta ttagccaaat tactattaga
     1261 aaaacctgat attctgctat tagatgaacc aactaaccat ttggatgctg agcatattga
     1321 atggttaaaa cgctatttac aacattatga aaatgctttt gtgttgattt cgcatgatat
     1381 ttctttctta aatgatgtga ttaatattgt ttatcatgtt gaaaatcaaa gtttagttcg
     1441 ctatactggg gattactacc aatttcaagc tgtttatgag atgaaacaat ctcaacttga
     1501 agcagcctat gaacgtcaac aaaaagagat tgctaacttg caggattttg tcaaccgaaa
     1561 taaggctcgg gtagcgacac gtaacatggc aatgtctcgc caaaaaaaac ttgataagat
     1621 ggatataatt gaacttcaag ctgagaaacc aaaaccaaat tttgaattta agcaagctag
     1681 aactcccagt cgattcattt ttcaaacaaa aaatcttgtg attggttatg attacccatt
     1741 gaccaaagaa cccttaaata taacgtttga aagaaatcaa aaaattgcta ttgttggggc
     1801 caacggtatt ggaaaatcta ctttgctaaa aagtttatta ggtgttattg agcctttaga
     1861 aggtcatatt gtcacagggg attttttaga agttggctac tttgaacaag aagtgacagg
     1921 tgttaaccga caaactccgc tagaagtagt ttgggatgct tttcctgcct taaatcaggc
     1981 agaagttcga gcggcactag ctcgttgcgg actaacatca aaacatatcg aaagtcaaat
     2041 tcaagtactt tcgggtggtg aacaagcaaa agttcgtttt tgtttgttga tgaatcgtga
     2101 aaataacgtg cttattttag acgaaccaac aaatcatctt gatattgatg ctaaaaatga
     2161 gctcaaacgt gctttaaaag catataaggg ttctatttta atggtttgtc atgaacctga
     2221 tttctacaat gggtgggtaa ccgatacttg ggattttagt aagttaacct aaactattcc
     2281 ttaatttttt acacagaaat tactaaaatc agtaaagata tagaataaat actgctagat
     2341 atgtgatggt ttaggtaact gatcaccaac caaaaaaaga tgtgactaca tcttttttta
     2401 tctaatgg
//
LOCUS       DAUELZ010000143         2233 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.144, whole genome shotgun sequence.
ACCESSION   DAUELZ010000143 DAUELZ010000000
VERSION     DAUELZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.144"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            39..518
                     /gene="rlmH"
                     /locus_tag="VHI09_001599"
     CDS             39..518
                     /gene="rlmH"
                     /locus_tag="VHI09_001599"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEQ6121451.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTP
                     DKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            complement(675..748)
                     /locus_tag="VHI09_001600"
     tRNA            complement(675..748)
                     /locus_tag="VHI09_001600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Arg,seq:tct)
     gene            954..1934
                     /locus_tag="VHI09_001601"
     CDS             954..1934
                     /locus_tag="VHI09_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEQ6121452.1"
                     /translation="MSNINSTRKSSYSHLSATERGEIAAYLKMGKKPVEIARLLGSHR
                     STICREIKRGSVDQVKDKNGKQTFFNAYFADSGQRVYETNRQKSSYLKLNGCSARFIA
                     QLESALTANIRLHSVDSFVQTYKVNHPEEVVPSTKTIYRYIKEGVLAIKPIDLPKMVS
                     IRKRSKKVMKTNKKTLGKSIEERPEYINDRSEFGHWEIDLVLGKKTKGEAVMLTLVER
                     QTRHALGVKLEDKQSQTINRAVTHLISQYPIASITADNGSEFSLLSTLEAVDVYFAHP
                     YSSHERRTNENFNGLLREYVPKGVSLNPLTSEELDNDITAINERLRRLLQ"
     gene            2130..2201
                     /locus_tag="VHI09_001602"
     tRNA            2130..2201
                     /locus_tag="VHI09_001602"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2163..2165,aa:Glu,seq:ttc)
BASE COUNT          762 a          408 c          408 g          655 t
ORIGIN      
        1 ttgtgtaaaa agtaatgttc ttgtgataca attaagctat gaaagtaaaa ctaatttgtg
       61 ttggaaaact aaaagaaaga tacctcaaag atggtatttc agaatatcaa aaaagacttt
      121 cccgtttttg tcaattcgaa atgattgagt taactgatga aagaacacca gataaagcaa
      181 gttttgctga taatcaattg attatgagta aagaagctca gcgaatccat aaaaaaattg
      241 gggaacgtga ttttgtaatt gcattagcta ttgaaggaaa acagtttcct tcagagacat
      301 ttagtgaatt aatatcaggg gttactgtta agggatattc aactatcacg tttattattg
      361 gtggaagtct tggattagat tcaattatta aaaaaagagc taatatgcta atgagttttg
      421 gattgttaac cttacctcat cagttaatgc gtctagtcct tactgagcaa atttatcgtg
      481 cttttatgat tacacaaggc agtccttatc ataagtaaga gagtgtgaac tggaataact
      541 aactcttacc actttattag ataatagttt ggtataatta aaagcaaata ttaaaaaaag
      601 acagactaaa tcctatcttt tcaatacact aaggtaaata caaaaaaagc actaaaacag
      661 tgctttttta tctttgatcc cagcaggatt cgaacctgcg accgttcgct tagaaggcga
      721 atgctctatc cagctgagct atgagacctt aaccacatca ttttaacaaa aattatctaa
      781 aactgtcaat atttatttta aaaaaactgg aaaataacat tatatcccgc gttgtaaaat
      841 gaagtgcaac aaaaaagaca tgatatcccg cgttgtaaaa tgaagtgcaa caaaaaagac
      901 atgttcgtct gatatactag aattccccaa ttcagtatgg aaagcagatg aacatgtcta
      961 atattaattc taccagaaaa tcatcctatt ctcatctttc agccacagaa cggggtgaaa
     1021 ttgctgctta tcttaaaatg ggaaagaaac ccgttgagat tgctcgactt ctaggtagtc
     1081 accgttctac aatctgccga gaaataaaac gtggttcagt agatcaggta aaggataaga
     1141 atggaaaaca aaccttcttc aacgcctatt tcgctgatag tggacaacgt gtctatgaaa
     1201 ccaatcgtca aaagagttct tacctcaaat tgaatggctg ctccgctagg ttcattgcac
     1261 aattagaatc tgccttgacg gctaacattc gtctccatag tgtggatagt tttgttcaga
     1321 cttacaaagt gaaccatcct gaagaagtcg tcccctctac caaaacgatt tatcgttata
     1381 tcaaagaggg ggtgttagct attaaaccaa tcgatttgcc taagatggtt agtattagaa
     1441 aacgttctaa gaaagtcatg aagacgaata agaagacttt aggtaaatct atcgaagaac
     1501 gtccagaata tattaatgat cgttctgaat ttgggcattg ggagattgat ttggttctcg
     1561 gcaagaaaac caaaggtgaa gctgttatgt tgactttggt agagcgccag acacgccatg
     1621 cactaggagt taagctagaa gacaagcagt cccaaaccat taaccgtgct gtcacgcatc
     1681 tcatcagtca gtatcctatt gcatccatca cagcggataa tggttctgag tttagtttac
     1741 tctcaacctt agaagctgtt gacgtttact ttgcacatcc ctattcttca catgagagaa
     1801 gaacaaacga gaacttcaat ggcctcctca gagaatatgt ccctaaagga gtctcactta
     1861 atccactaac ctctgaagaa cttgacaatg atattactgc catcaatgag cgcctaagac
     1921 gacttcttca atagcaatcc tcaaaattcc tgtttgagct atcccgaaca gcttaacctc
     1981 tggaactcta gttatctatg aactcgttgc acttgacttg acaacttgcg gtttaaaaaa
     2041 aatcaaattt tttttaaaaa aagtatagac aaaaaaaatc aatctgctat aatagtaaga
     2101 gtgttaagga gatatcctcc aaaacttatg gtccgttggt caaggggtta agacaccgcc
     2161 ttttcacggc ggtaacacgg gttcgaatcc cgtacggact atcgcaattg tcattttggc
     2221 aatttttttc ttt
//
LOCUS       DAUELZ010000144         2215 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.145, whole genome shotgun sequence.
ACCESSION   DAUELZ010000144 DAUELZ010000000
VERSION     DAUELZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.145"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            194..838
                     /locus_tag="VHI09_001603"
     CDS             194..838
                     /locus_tag="VHI09_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEQ6121453.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQKVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
     gene            848..1543
                     /locus_tag="VHI09_001604"
     CDS             848..1543
                     /locus_tag="VHI09_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEQ6121454.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTVAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(1545..2192)
                     /gene="radC"
                     /locus_tag="VHI09_001605"
     CDS             complement(1545..2192)
                     /gene="radC"
                     /locus_tag="VHI09_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEQ6121455.1"
                     /translation="MPRERLMRLGAESLSNQELLAILLRTGNKEKHVLELSSYLLSHL
                     DSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTDSVLTSVQVAEK
                     MMAALGDKKQEHLVVLYLDNQNRIIEEKTIFIGTVRRSLAEPREILYYACKNMATSLI
                     VIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYSFREKSTLF"
BASE COUNT          688 a          413 c          376 g          738 t
ORIGIN      
        1 tattgctatt aattcatgtt gttcattttg actgtcataa cttactttcc taatgaatac
       61 tattatatag tgctaaaact aattttagct tttttaactc atacaaaaac aatcaaaatg
      121 ttgcattttt cacaatcttt ttgttacaat aaagacagac atatttaacg agaaatctac
      181 aaggagtaaa atagtggtta ttgacaaatc tattccaaaa gcgactgcaa agcgcttatc
      241 actttattac cgtattttta aacgattcca tgctgaccaa gttgaaaaag ccagttctaa
      301 acaaattgca gatgcaatgg gaattgactc tgctactgta cgccgtgact tttcttattt
      361 tggagaattg gggcgtcgtg ggtttggcta tgatgttacc aaattgatga atttttttgc
      421 tgacttgctt aatgatcact ctacaacaaa cgttatctta gttggatgcg gtaatatagg
      481 gcgtgctcta ttacattacc gcttccatga tcgtaataag atgcaaattg ccatgggttt
      541 tgataccgat gataacgctc ttgttggtac taaaacagct gataatattc cagtccatgg
      601 tatctcatct gtcaaagaac ggattgcgaa cactgacatt gaaacggcta tcttaacggt
      661 tcccagcatt catgctcaaa aagttactga tcaacttatt gaagcaggaa tcaaaggaat
      721 cttgagtttt gctcctgttc acctacaagt tcctaaagga gttattgttc aatctgtcga
      781 tttgaccagt gagctccaga cactactcta ttttatgaat caaaatcatc tagattaagg
      841 aggtataatg accaaaccga ttattggaat aactgcaaat cagagactaa atatggcttt
      901 ggataatctc ccatggtcat atgcacctac tggatttgta caggcagtca ctcaatctgg
      961 ggggctgccg cttcttttac ccattggtga tgaagccgcc gctaaaactt atgtatctat
     1021 ggttgataaa atcattttaa ttggtgggca aaacgttgac ccaaaatatt atcaagaaga
     1081 gaaggctgct tttgatgatg atttttcacc agagcgagac acatttgagc tagctatcat
     1141 caaggaggct ataacattaa aaaaaccaat cttagggatt tgtcgtggta cacagttaat
     1201 gaatgttgcc ttaggaggaa atcttaacca acacattgac tcacattggc aagaagcgcc
     1261 ttctgatttc ttatcacacg aaatgattat tgagccagac tctatcttat atcctattta
     1321 tggtcataag acattaatta attcttttca tcgccaaagt ttaaaaactg ttgctaagga
     1381 tttaaaagta atcgctagag atccacgtga tggtaccatc gaagcagtca tatcaaccaa
     1441 cgatgccatt ccttttttag gtgttcagtg gcatcctgaa cttctccaag gtgttcgaga
     1501 tgaagatcta caattgttcc gcttattcgt caatgacttc tgacttaaaa aagggttgat
     1561 ttttctcgaa aactataata atctttatag ctaacgataa tgtgatctag acagataatg
     1621 cctaaatctt cacatgatcg ttttattttt tcagtaaagc aataatcgtt agaactaggt
     1681 tcaatatttc ctgaaggatg attatgaata acaatgagac tagtcgccat atttttacag
     1741 gcatagtata aaatttctct tggttctgca agtgaacgtc ggacagtccc aataaaaata
     1801 gttttttctt caataatacg attttgatta tctaaataca atacgactaa atgctcttgt
     1861 tttttatctc ctaaagctgc catcattttt tcagcgacct gaacactggt taatacgcta
     1921 tcagttaatg tcttatctgt cgctaaaatt cgggaaacca actcaatcat agctttaatt
     1981 tcaatcgctt taacttttcc tatacctgcc aaatgttgta attcttgcaa agacatcttt
     2041 ttaaaatctg ccagactgtc taaatgcgat aaaagatagg atgacagctc taagacatgc
     2101 ttttctttat tacctgttcg taataaaatt gctaataatt cttgattact tagagactct
     2161 gctcctagtc gcatcaaacg ttctcttggc atggctttat tatcatcaca tttta
//
LOCUS       DAUELZ010000145         2175 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.146, whole genome shotgun sequence.
ACCESSION   DAUELZ010000145 DAUELZ010000000
VERSION     DAUELZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.146"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            357..1073
                     /locus_tag="VHI09_001606"
     CDS             357..1073
                     /locus_tag="VHI09_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ6121456.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            1276..2118
                     /locus_tag="VHI09_001607"
     CDS             1276..2118
                     /locus_tag="VHI09_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ6121457.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYKVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLTKEHLGGDYVPSDKELKL
                     PTAN"
BASE COUNT          763 a          342 c          444 g          626 t
ORIGIN      
        1 gaacaggcta acgtttttac aagattatat cagtaatcat tttgacaatt gtagtaatgg
       61 aatgttgcca aaatttagaa cgttgtgtgt tatgaatgac aagattttaa aggaatacta
      121 attaaacggg aggtcgtgaa ccggaagctg ttgtcactca agcgctaaaa tctgtctgta
      181 acataatatt gatgtatggg aacgcaatta tttggttttt attgtgatgc ttaaaacgat
      241 tttatatttc tattttacca aagcttttca aagtgctata ataagggagt tagagtcaat
      301 attagtaacg ttaatgttga caatattcaa taaaatacta ttaaaaggag acaaaaatgg
      361 gacgtaaatg ggcaaatatt gttgctaaaa aaactgctaa agatggagca acatcaaaga
      421 tctatgccaa atttggtgtt gaaatttatg tggctgccaa acaaggcgag ccagatccag
      481 agttaaatac agctcttaaa tttgtcattg atcgtgcaaa gcaagcacaa gtgccaaagc
      541 atgttattga taaagcgatt gataaagcta aaggaaacac agatgaaact ttcgtagagg
      601 gacgctatga aggttttggt ccaaatggtt caatgattat tgtggatact ttgacatcaa
      661 atgttaaccg tacagcggca aatgtacgta cggcttacgg taagaacggt ggcaatatgg
      721 gagcttcagg atctgtatcc tacttatttg ataaaaaagg ggtcatcgtt tttgctggtg
      781 atgatgctga cagtgtcttt gagcaattac ttgaagcgga tgtagacgta gatgatgttg
      841 aagcagaaga gggaacaata acagtttaca ctgccccaac agatcttcat aaaggtatcc
      901 aagcacttcg cgataatggt gtagaagaat tccaagttac tgaacttgaa atgattcctc
      961 aatcagaagt agtattggaa ggtgatgacc ttgaaacttt tgaaaagctt attgatgcac
     1021 tcgaatcaga tgatgatgtt caaaaagtgt atcataatgt agcagatttt taaattgaca
     1081 tagaataata aaaagagttg aaatctattt cagctctttt taagtgatgc ttttataagt
     1141 aagtaccagg taacatagtc gttgttagaa gataatgaac aatagtaaat cagtatcaac
     1201 ttgataggaa tcaattatta gtgaactcta attaaaaatg gtaaactaga cacaagaaca
     1261 aaaaggagag ataggatgat taaaaaacag tatttaggaa gggcaacaat tgccctagca
     1321 tcgacccttg tgctagcagc ttgtgggtca tcaaaaacag ctgagtcagg aaatcagggg
     1381 agttctaaag aagtcttatt tgcaacggtt ggaacaacag ctcctttttc ttatgaaaaa
     1441 ggtggtcaac taactggcta cgatatcgag gtggctaaag cagtctttaa aggatcggat
     1501 gattacaagg tgtcctttaa aaaaacagaa tggtcttcta tctttacagg tttggactca
     1561 ggaaaatacc aaatgggtgg taacaacatt tcctttacca aagagcgttc agctaagtac
     1621 cttttttctt atccaatcgg atcaacacct tctgttttag ttgttccaaa agacagtgat
     1681 atcaagagct ttgatgatat ccaagggcat acgacacaag ttgttcaagg gactacaagt
     1741 gttgcacagt tagaagactt taataagaaa cacagtgaca acccggttac tctaaaattc
     1801 accaatgaga acattactca aatgttaacc aatttgagtg aaggcaaggc agatttcaag
     1861 atttttgacg ctccaacggt taacgccatt attaaaaatc aagggttaga taatctcaaa
     1921 acaattgaac tcacctcaac agagcaacca tttatttatt ttatctttag tcaagatcaa
     1981 gagaaattgc aaagctttgt taacaagcgt attaaagagt tgactgctga cggaactctt
     2041 agcaagctta ccaaagagca ccttggcggt gattacgtgc catcagataa agagttgaaa
     2101 cttcctacag ctaactaaac catctattaa ccagttattc atctagaaga ctggtttttc
     2161 tgatcggaaa caata
//
LOCUS       DAUELZ010000146         2109 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.147, whole genome shotgun sequence.
ACCESSION   DAUELZ010000146 DAUELZ010000000
VERSION     DAUELZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.147"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(1..1334)
                     /locus_tag="VHI09_001608"
                     /pseudo
     CDS             complement(1..1334)
                     /locus_tag="VHI09_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
     gene            complement(1345..>2109)
                     /locus_tag="VHI09_001609"
     CDS             complement(1345..>2109)
                     /locus_tag="VHI09_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ6121458.1"
                     /translation="VDSEFQEAGTLVNQELLHGNIQFEELSYKYGFGRDTLSNINLTI
                     KQGDKVSLVGISGSGKTTLAKMIVNFFEPYNGRITINHNDLKMIDKKSLRQHINYLPQ
                     QAYIFNGSILENLTLGANDCTSHEDILRACEVAEIRQDIEQMPMGYQTELSDGAGLSG
                     GQKQRIALARALLTKAPVLILDEATSGLDVLTEKRVIDNLLAMTDKTIIFVAHRLSIS
                     ARTNQVIVLDEGKIIETGSHQELMTKQGFYHHLFSG"
BASE COUNT          610 a          340 c          389 g          770 t
ORIGIN      
        1 tagtaattga aatagctttt ccgtgtaatg acacttgtta cacgtccttg caatccatac
       61 tgtacgacgt cactgtcctt attagaaagt ttggagagtg cagtaacctt gaagagatta
      121 ccttgatcag tctttgtggc agatttatca atagtctgaa tcgttccgtt aattgttaga
      181 gtttggtttc caactttttc aagactaaga cgaacacctt gcttttcttt taacaatgag
      241 acatgctctg aagtgacgta gtaggtaatg aatacttcct ttattttttg gatatctggg
      301 tagatttgag ccacctcact tccattagga atgagagatt tcccttcaaa ttcactgttt
      361 agatgtatcg ttcctgtttc aggagctata attatattgt tctttagctg aatattggct
      421 tgctcaattt gatctttaag ttcagtaatt tgattttcaa ctgtggtaag ttgttgggaa
      481 gcattttgca gaaattgtgt ttttaaaata tctattttga tcctatgact agtgtctgga
      541 agagataaat ttcctgtact agcacgttgg atactaaggt ttgccattga agattcaagt
      601 tctgagatat tttgtgagat ttgagaaagg tattcttctt ttaaagctga gttatcttct
      661 tgtgcttgaa attgagtttg atattttttt agggcagttt gatgtggatt ctcaggtgaa
      721 agcttatatt cttgattatt aatagctcgc tttagctctt cataatctgt aattttttga
      781 tgaagttcgt caagctgact agtaataact gaaatggcat gattagcaag acttgcttga
      841 ttattgactt cagcattttt tttagcaaac ccaatgatta tatcttgggt ttgctaaaaa
      901 aaatgattaa acgtatcgct atagccgaat tcatcttctc ccttaaataa gttagtttct
      961 tgttctagac tggttttgag tatttggagt ccattttttt gtttgttaag agtggctaat
     1021 tgtttttcta aagctttttt ttgagaattt tccattgttt ttgagtattg gataataaca
     1081 tcacctttct taataaattt attgtttttt aaattgttga cgacaatagt atggtcacta
     1141 gttgattgga ctgaagcaat aacttttgtt ggcgtcattt ctccttgtga agtaatggta
     1201 atttcttttt tagcaaagag agaaaaaaga actaagaagc tcactaacaa aacggatggc
     1261 aaaatcaaga aagttgcaaa gttatgatga cgtcgatgat aaaattccgc acttttaaag
     1321 aggtttggat tcatgttatt ctccttaacc actaaataaa tgatgataga aaccttgttt
     1381 agtcattaat tcttgatgag atcctgtttc aataattttg ccctcatcaa gcacaatgac
     1441 ttgattggtc cgcgctgaaa tgctgagacg atgagctacg aaaataatag ttttatccgt
     1501 catagccaaa agattatcaa taaccctctt ttcagttagt acgtcaagac cgcttgtagc
     1561 ctcatctaga ataagcacag gcgccttggt taggagagct ctggcaagcg caattcgttg
     1621 cttttggcct cctgagagtc cggcaccatc agaaagctct gtttgatagc ccataggcat
     1681 ttgttcaata tcttgtcgaa tttctgcaac ctcacaagct cttagaatat cttcatgact
     1741 agtacagtca ttagcaccta aggttaaatt ttctaaaata gaaccattaa aaatataggc
     1801 ttgttggggg agataattaa tatgttggcg aagacttttt ttatcaatca tttttaagtc
     1861 attatgatta atggtaattc ggccgttata aggctcaaaa aaattaacaa tcattttagc
     1921 tagagtcgtt ttaccagatc cactaatacc aacaaggcta actttgtccc cttgcttaat
     1981 agtgagatta atatttgata gagtatcgcg tccaaagcca tatttatagg ataactcttc
     2041 aaattgaatg tttccgtgta ataattcttg attgactaat gtcccagcct cttgaaattc
     2101 agaatcgac
//
LOCUS       DAUELZ010000147         2073 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.148, whole genome shotgun sequence.
ACCESSION   DAUELZ010000147 DAUELZ010000000
VERSION     DAUELZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.148"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            82..2073
                     /gene="uvrB"
                     /locus_tag="VHI09_001610"
     CDS             82..2073
                     /gene="uvrB"
                     /locus_tag="VHI09_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEQ6121459.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRSSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGIFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
BASE COUNT          686 a          353 c          456 g          578 t
ORIGIN      
        1 ttttttacgc ttattggagg tgattctggc ttatctatgt taaaatagtt tataagagat
       61 aagactatta gagggtataa aatgattgat aaacgtgatg ataaaccatt taaactaaaa
      121 tcaaagtata aaccgtctgg tgatcagcca caagctattg aaagcctagt tgataatatc
      181 gagggcggag aaaaagctca gattctccta ggtgctacag gaacgggaaa aacatacacc
      241 atgagccaag taattagtaa agtaaataaa cctaccttag tgatagctca caataaaacc
      301 ttagcaggac aattatatgg tgaatttaaa gaattttttc cagacaatgc tgtggaatac
      361 tttgtttcct attatgacta ctaccaacct gaagcatatg tcccatcaag cgacacctat
      421 atcgaaaaag atagttcagt caatgatgaa atcgataaac ttcgccattc agccacctct
      481 tctttacttg agcgtaacga tgtcatagtg gtagcttctg tatcttgtat ttatggacta
      541 ggttcgccaa aagagtacgc agattcagca gtcagcctac gtccaggtca ggagatatca
      601 cgtgacactt tgctaaatca attagtagat attcaatttg agcgtaatga tattgatttt
      661 caacgcggct gttttcgtgt acgtggcgat gttgttgagg tctttccagc atccagggat
      721 gaacatgctt ttcgggtgga atttttcggg gatgagattg accgtatatg tgaaatcgaa
      781 agtttaactg ggaaaactat tggagaagtt gaccatttgg ttttatttcc agctactcac
      841 tttgtaacaa atgatgagca tatggagcaa tcaatagcca aaattcaagc agagttagca
      901 gagcagttac agttatttga atctgaaggt aaactactag aagcacagcg attacgccaa
      961 agaacggaat atgatattga aatgttacgt gaaatgggct atactagcgg agtcgaaaac
     1021 tattcccgtc atatggatgg caggtcacca ggagaacctc cttatacctt gttagatttc
     1081 ttcccagaag attttttaat tatgattgat gaaagtcaca tgacaatggg acaaatcaaa
     1141 ggcatgtata atggtgacca agccagaaaa caaatgctag ttgactatgg ttttcgattg
     1201 ccatcagctc ttgataatcg tcccttaaga cgcgaggaat ttgaaagtca tgtgcaccaa
     1261 attgtctatg tttcagcaac gcctggtgaa tacgaaatgt cacagactaa tacaattatt
     1321 gagcaaatta ttcgtccgac tgggctactt gatcctgaaa ttgatgtaag gtcaagtatg
     1381 gggcaaatgg atgatttgtt gggagaaatt aaccagcgcg tagcgcgcga tgaacggacg
     1441 ttcatcacaa ccttaactaa aaaaatggca gaagatttga ctgattatct aaaagagatg
     1501 ggtgtcaagg tcaagtatat gcacagtgat attaaaacct tagagcggac agaaattatt
     1561 cgtgatttac gacttggtat ttttgatgtc ttgattggaa tcaatctgct ccgtgaaggg
     1621 attgatgtgc cagaagttag tcttgtagcc attttggatg ctgataaaga gggtttcctc
     1681 cgtaatgaac gcggcttgat tcagaccatc ggtagagcag ctcgaaacgt agatggtcat
     1741 gtcattatgt atgcggacaa gatgacagat tctatgcagc gggctattga cgaaacggct
     1801 cgtcgtcgtg agattcaaat agcatataac aaagcgcacg gcatcgttcc acaaactata
     1861 aaaaaagaca tcagaggcct tattagtata agtaaaacaa gccacaatga tatatcaaaa
     1921 gaagagatgg attatgaaag tatgtcaaga ggagaacgaa aagaagcgat caatgctctg
     1981 caaaaacaaa tgcaagaggc agctgaatta cttgatttcg aattggcagc tcaaatgcga
     2041 gacttgattt tagaacttaa attaatggac tag
//
LOCUS       DAUELZ010000148         1994 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.149, whole genome shotgun sequence.
ACCESSION   DAUELZ010000148 DAUELZ010000000
VERSION     DAUELZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.149"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..290
                     /locus_tag="VHI09_001611"
     CDS             <1..290
                     /locus_tag="VHI09_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ6121460.1"
                     /translation="IQKAPIFGIMKAQGISNKTITTAVLMQTFFLSFLGSGLGLLGTW
                     LTSLLLPTVVPFQSNWFLYLAIFVSMICFALLGTLFSVFNIIRIDPLKAIG"
     gene            300..968
                     /locus_tag="VHI09_001612"
     CDS             300..968
                     /locus_tag="VHI09_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121461.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGCLQTPSSGQLIIDGTDYTHLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGITGLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLKYCDKVYRMQDGEL
                     CQER"
     gene            complement(1070..1987)
                     /locus_tag="VHI09_001613"
     CDS             complement(1070..1987)
                     /locus_tag="VHI09_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEQ6121462.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSGVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRTVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
BASE COUNT          580 a          413 c          405 g          596 t
ORIGIN      
        1 ctattcaaaa ggcacctatt tttgggatta tgaaagcgca aggaatttct aacaaaacga
       61 ttacgaccgc tgtgcttatg cagacattct ttttgagttt tttaggtagt ggtttagggt
      121 tgctaggtac ttggctgaca tcattattat taccaacagt agtacctttt caaagcaatt
      181 ggtttttgta tttggctata ttcgttagta tgatctgttt tgctctatta ggcaccttat
      241 tttctgtttt taacattata cgaattgatc ctttgaaagc aattggatag gaggtagtga
      301 tgtcggtttt aacatttaaa caagtgacca aaacatttca agatggtcat cacgaaatca
      361 acgcattaaa ggcaactgac ttttccattg aagctggaga atttgtggct attattggac
      421 catcaggttc tggaaaatca acctttttga ccattgctgg ttgcttacag accccaagtt
      481 ctggtcagct aatcattgat ggcactgatt atacgcattt atcagaaaaa gagcgctcac
      541 gcttgcgctt caaatccgtt ggttttatct tacaagcttc caatctgata ccttttttaa
      601 cggtccagca gcagttggaa ttggtcgatc atttgacagg aagcaaagaa aaagccaaag
      661 ccaatcagtt atttgacgat ttaggcatta cagggctaaa acatcagtta cctcaagaat
      721 tatccggcgg agaaaggcaa agagcggcta ttgcccgtgc tctttaccat gatccagcac
      781 tgatattggc agacgaacct actgcaagtt tggatactga aaaagcctat gaagtggtca
      841 aactgttggc taaggaaagt aaagaaaaaa acaaggccat tattatggtc actcatgatg
      901 atcggatgct aaaatattgt gacaaagtct atcggatgca agatggagag ttatgtcaag
      961 agagatagta gactggtaag agtttatgat attagaaaaa aaccaaacaa gcacagcact
     1021 tgtttggttt ttttctaagt gtgactatta tcaatacgtc accatttccc taaagcttac
     1081 tatacgggat gctaaaggta tcaccatgct caaagtcgcc atattggtaa cctcggtcaa
     1141 accaacgttg gcgttgctta gaagtgccat gtgtaaaact atcaggaacg gttctgccat
     1201 aggtttcttc ttgcagagta tcatccccaa ctgcgtgagc tgctgccatt gcctcctcaa
     1261 tgtctccttt ttccaaaagg ccttgccctt gaacataatt agcccaggca ccagcatagt
     1321 aatcagcttg taattccaac ttgacgttga gttgattggc tttagccttt gattttcctt
     1381 gtctagcgct agcgtaatta tccataattc ccaattcatt ttggatgtgg tggccaactt
     1441 catgagcaat cacataggcc atagcaaaat caccttttgc gccgtacttg gttgatagtt
     1501 cattataaaa agaaatatct aaatacactt tttgatcgcc tgggcaataa aaaggaccag
     1561 aagaggcttg ccccctgcca caagcggttt gagttgctcc agtatataga actaatgttg
     1621 gtttatgata agttaaccct ttctcacgaa atgtcttagt ccaatagtcc tcggtagagg
     1681 cgaagacctt actgacaaaa gaaacctgtt cttgactagc cttgtcacca tttgtacgcg
     1741 ttacttggct tgattgatag gcattgttgt tggtagaaga aggcttgcct cctaaaacac
     1801 cagataggcc acctcctccc ataactagga gaaggagaag aataaccagc ttggttttcc
     1861 aacctcctcg tgaaaagagt aattgaagca ggagaccgcc ccctaggccg ccgcccccaa
     1921 aagaaccaga cgattgacca cgtctatctt caacctgttg actttctctc aaatcatctg
     1981 ttttcataag tttc
//
LOCUS       DAUELZ010000149         1991 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.150, whole genome shotgun sequence.
ACCESSION   DAUELZ010000149 DAUELZ010000000
VERSION     DAUELZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.150"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..766)
                     /locus_tag="VHI09_001614"
     CDS             complement(<1..766)
                     /locus_tag="VHI09_001614"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEQ6121463.1"
                     /translation="MKRIIVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKR"
     gene            856..>1991
                     /locus_tag="VHI09_001615"
     CDS             856..>1991
                     /locus_tag="VHI09_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEQ6121464.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYSTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPHFLTQKSLIDLDTNLLMQKVLALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTSDSY"
BASE COUNT          655 a          371 c          312 g          653 t
ORIGIN      
        1 ctctttttaa tgtaagggta ttttgcaata ctttttcaag aaatgaaaca acaagatcta
       61 gaatttgtgt tagtggtagc tgataataga atcctgctgt tataatagct gatgctatat
      121 ctcctgtacc atagaaatgg taagggaatt gcttcccaaa atggaatgtt aattgatctg
      181 aatctgactg atagtaagca aaaccaatgc tgccctcctc aaaagagatt ccgctaatga
      241 caacctgttt aggtccaagc ttagctaatt ttctagccac ctgtctaata tcttcttcat
      301 cataattttt tgctaaataa gctgtttgtg ttaatagaca agcctcacta atattaggta
      361 aaacaagctc agctttttga gtaagtttta tcataatctc tatatgtttt tgactgaagc
      421 cttgataaag ttttccatta tctgccataa ttggatctaa aattaaggga agttgctttc
      481 ttgaaagcca ttctagcagc ttttgcccat ctcttgttgt cgtaaaataa cctataaaaa
      541 tgccgtcaaa cttaattctt ttgttgtccc aatctgttaa aaaaacgtct aatatacttg
      601 atgtagtatg cttcacaggg ctctgtcctc cgccagtatg gcttgataaa aggcaagttg
      661 gtaataaaac ttgctctaac aaacaagaag ccattaaagg aatactagca gacaaagcta
      721 ccttaccaac accaaccaga tcatttgcca caataatccg tttcatagct attacctctt
      781 ttctataata actattgtaa gaaaaagagg cataagatac aatagttata acacttaact
      841 gtatggggac aatttatgac aacaaaatat caaacaatca tttccaatat cgaacaagat
      901 atccagaaac agcgactaaa aaaaggggac aagctacctt caattagggt attaagtaag
      961 gtttatcatt gtagcaaaga tactgttcag cgggctcttt tagaattaaa atatcgtcac
     1021 ttaatttatg ctgttcctaa aagtggttat tatgttttgg gtaacgtaag catgcccgat
     1081 aatgttctta atctaagtct tgaagattat aataatatgg cttatgaaga tttccgactc
     1141 tgcttaaatg aggctttaag tgctaaagac aaatacctct ttcattatta tcacaaaacc
     1201 gaaggactag aagaactaag ggaagctttg cttctttact tagctgaaaa tagtgtttac
     1261 agtaacaaag accaactatt aatcacctct gggacgcagc aagcgcttta tattttatca
     1321 caaatgcctt ttcctaatgc gggcaaaaca attctccttg aaaagcctac ttatcatcgg
     1381 atggaagcta ttgttgctca gttaggatta ccctatcaaa ctatctctag acactttaat
     1441 ggccttgatt tagaactctt agaatcttta tttcaaactg gtgatattaa atttttttac
     1501 acgatttcac gattttcaca tcctctggga ctttcttata gcactaagga aaaggaagct
     1561 attgttcgtc tagcacaacg ataccaggtt tatattttag aagatgatta tttaggagat
     1621 tttgtgaaac ttaaagagcc accaattcat tattatgaca ctcatcaccg aatcatctac
     1681 ctaaaatctt tttcaatgag tgtttttcct gcactccgta tcggagctct agttttaccg
     1741 tcaggtttaa aacctcattt tttaacccaa aaatcactga ttgatttaga cacgaactta
     1801 ctaatgcaaa aagtattagc tctttattta gaaaatggga tgtttcaaaa aaatcttcgc
     1861 tttattaagc gttatctaaa acagcgggaa cgtcaattgg ctctcttttt aaaacaaaat
     1921 tgccctgata ttcactatca actaacccct actcatttag tgattgatta cacgacatca
     1981 gactcttata g
//
LOCUS       DAUELZ010000150         1906 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.151, whole genome shotgun sequence.
ACCESSION   DAUELZ010000150 DAUELZ010000000
VERSION     DAUELZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.151"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            371..1366
                     /locus_tag="VHI09_001616"
                     /pseudo
     CDS             371..1366
                     /locus_tag="VHI09_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1347..>1906
                     /locus_tag="VHI09_001617"
     CDS             1347..>1906
                     /locus_tag="VHI09_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LlaJI family restriction endonuclease"
                     /protein_id="HEQ6121465.1"
                     /translation="MLTVPNKSLLDMCRVATNMEGDTFVGIKAEIIEGVQEVTVNFPL
                     GFEIAKDVETARRDIIALVSVLQAHNDEESRLRTVNVNQALKTLNFPMEAYTFVIHDF
                     INRGTYYKETEEYYIQTLGGRINWARTIKRVQPVVQGNGFKFLKMESKKQSDTDITLL
                     TEINKFCVYEAFLNMGWLYQLPQPEKA"
BASE COUNT          659 a          269 c          380 g          598 t
ORIGIN      
        1 gttggacaca ctatcgggcg atgattttaa agaattttgc caaacagcta gaaatggtga
       61 attagatgaa aatttatttg atactatctc agtagatctt actgaaactg atacgttatt
      121 tctaaagaaa tttgttaccg attatcattg gtggcatggc gggaaggaaa tccacagggg
      181 ggatagtttt atatcaccaa ttttagcttt attaaagtta attaatgctt cttctgaaca
      241 tgtagcaata attgtaaagt atctcgttga tgcagatttt gatttaagtg atgtaaataa
      301 gtactttgaa cttgttgaaa tggacagccg aagtgtagtt actaccaatt atgacaatac
      361 gaaccttgtg ggtgataata ttcttctcta tggggttcct gggtctggaa aaagttttaa
      421 aattgctacc gaatattgca atgatgaaaa tagaatggag cgattagtct ttcatccgga
      481 ctatatgaat actgactttg tcggacaaat aatgcctact gtaaaagaaa atggagatat
      541 tacctatctt ttttcacctg gaccttttac aagaattgtg aaaaaggcaa ttgataaccc
      601 tagtgaacac tattatcttg taattgagga gttaaatcga ggcaatgctc ccggaatatt
      661 tggagaaatc tttcaattat tggatagaag tgctagcggt gaaagcgtct acggtattaa
      721 caattcaata cttgctacgg aaatttatgg tgataagtct catcaaatta aaatcccttc
      781 taacttatcg ttacttgcaa caatgaatac cgcagaccaa aatgtattca ctcttgatac
      841 agcatttcaa cgtcgatgga caatggaaat gattgaaaat gatgttgatt tatcggaata
      901 tgggaattat tttattgctg atacacaata ttcttggaaa gtattcaaca aagttatcaa
      961 taaaaaaatt cttgagacta gtagccagac tctaacttca gaagataagc gaatgggagc
     1021 atattttatt tctggtaatg tattgaatat tgaacggata catcaagtac cagcaggtga
     1081 ttccggtaat gaattcaata aaaaaaataa acgctttgca gaaaaagttt tgaaatatct
     1141 ttgggatgat gccttcaagt ttaatcgtga agatatcttt gatgttgctt tgtatccaag
     1201 tttagaggca attattaagc gattcacgga taatgataat cctgaaaatg ggcgttttga
     1261 tgttttcgcc aaagatatca aagaggagtt agaaaatctt aaggaagcaa ctttaataga
     1321 taataacaac gacacaggag aagaagatgt tgacagtacc gaataaatcc ttactggata
     1381 tgtgtcgtgt tgcaacaaat atggaaggtg atacgtttgt tggtattaaa gctgagataa
     1441 tagaaggtgt acaagaagtt actgtaaatt ttccattagg gtttgagatt gcaaaagatg
     1501 tagaaacagc aagaagagac ataattgccc ttgtttccgt cttacaagct cataatgatg
     1561 aagagtctcg cctaagaact gtaaacgtga atcaagctct aaaaacgtta aactttccta
     1621 tggaggctta tacatttgtt atacatgatt ttattaatcg tggtacatat tacaaggaga
     1681 ctgaagagta ctatattcaa actttgggtg gccgaattaa ttgggcgaga actataaaac
     1741 gtgttcaacc agttgttcaa ggaaatggat ttaaattttt gaaaatggaa tctaagaagc
     1801 aaagtgatac tgatattact cttttaacgg aaataaacaa attctgtgta tatgaagctt
     1861 ttctaaatat gggatggctg tatcagcttc ctcaacctga aaaagc
//
LOCUS       DAUELZ010000151         1868 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.152, whole genome shotgun sequence.
ACCESSION   DAUELZ010000151 DAUELZ010000000
VERSION     DAUELZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.152"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..484
                     /locus_tag="VHI09_001618"
     CDS             <1..484
                     /locus_tag="VHI09_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA helicase"
                     /protein_id="HEQ6121466.1"
                     /translation="HRQKLPPKILKYLKKQRKTGYPLLIFLPTILMAESVTAILKELL
                     PAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYS
                     SQSLVQIAGRVGRSIDRPDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN
                     "
     gene            456..1121
                     /locus_tag="VHI09_001619"
     CDS             456..1121
                     /locus_tag="VHI09_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEQ6121467.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVNPGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            1201..1749
                     /gene="raiA"
                     /locus_tag="VHI09_001620"
     CDS             1201..1749
                     /gene="raiA"
                     /locus_tag="VHI09_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEQ6121468.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT          691 a          293 c          339 g          545 t
ORIGIN      
        1 acatcggcaa aaacttcctc ctaaaatttt aaaatactta aaaaaacaaa gaaaaacagg
       61 ctatcccctt ttaatttttc tacccactat tctaatggca gaatctgtta cagcaatctt
      121 aaaagagtta ttgcccgccg aacaaatagc ttgtgtttct agtcaatcac aaaacaggaa
      181 agaagatatc acagcttttc gtcaagggaa aaaaacgatt ttaattacaa caacaatttt
      241 agaaaggggt gtgacatttc cccaaattga tgtttttgtt cttgggtctc atcatcgtgt
      301 ctatagttcc caaagtcttg ttcaaattgc tggacgagtt ggaagatcta tagacagacc
      361 agacggaacg ttatatttct ttcatgaagg tatcagtaaa gcaatgctac tagcaagaaa
      421 agagattaaa gaaatgaatt ataaagggta ttcgcatgat ctgtctacta tgtcaacaaa
      481 ttagtcaaac accaataagt attacagaaa tcatcttttt aaggcgtatc tcttcaccga
      541 tttgtcaaca atgtcaaaaa agctttcaaa agataggaaa aagtgtttgt gcgacatgtt
      601 gtgcaaactc agatataata gcttgtcgag attgtctaaa atgggaaaac aaaggataca
      661 atgtaaatca tagaagctta tattgttata atgctgctat gaaagcatac ttcagtcaat
      721 ataagtttca aggagactat ttattaagaa aagtttttgc agtagaactt gccgatgtta
      781 ttaccaagta ctataaaggc tatatcccag tcccggttcc tgtaaatccc ggttgttttc
      841 gagaaagaca atttaatcaa gtgagcgcta ttcttgaggc agctaatgtt agctaccttt
      901 ctctttttga aaagctagat aatactcacc aatcttccag aacaaaaaaa gagcgattat
      961 tagtagaaaa atcttatcga ctactaaaag tatcaaacat tcctgataaa atccttatag
     1021 tagatgatat ttatactact ggtagtacaa ttatcgctct tagaaaacaa ttggctaaag
     1081 tagcaaatag tgacattaaa agtttgtcaa ttgcacgtta aaacttgcaa attttcatga
     1141 aagcagtata atagtattaa agataaagta agcgcttcaa gcgcgagaaa gaggtatcct
     1201 atgattaagt tcagtattcg tggagaaaat atcgaagtga cagaagctat tcgcgattat
     1261 gtagaatcaa agcttactaa aatcgaaaaa tattttgcta aagaccaaga aatagatgca
     1321 cgtgttaatt taaaagttta tcgggagaga tcttcaaaag ttgaggtaac gatcccatta
     1381 gattcagtaa cccttcgtgc tgaagatgtt tcacaggata tgtacggctc tattgattta
     1441 gttgttgata agatcgaacg tcaaattaga aaaaataaaa ccaaaattgc taaaaaacat
     1501 cgcgaaaagg ttccaactgg tcaagtcttt acaactgagt ttgaagctga agaagtcgat
     1561 gagataccag aagtacaagt tgtacgtact aaaaatgtga cattaaaacc aatggatgtt
     1621 gaagaagcgc gtttacagat ggaactattg ggacatgatt tctttatcta cactgattct
     1681 gaagatggtg caacaaacat tttataccgt cgcgaagatg gaaacctagg tttaatagaa
     1741 gctaaatagt tcacaattaa aacctacagg gatgattata cgtattttaa aagtgggaaa
     1801 aagctattca tgtttagagg tgacatgatg aaaatgagat taagtgatag atacgacagt
     1861 cattatac
//
LOCUS       DAUELZ010000152         1834 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.153, whole genome shotgun sequence.
ACCESSION   DAUELZ010000152 DAUELZ010000000
VERSION     DAUELZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.153"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(321..392)
                     /locus_tag="VHI09_001621"
     tRNA            complement(321..392)
                     /locus_tag="VHI09_001621"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(357..359),aa:Arg,seq:ccg)
     gene            complement(505..1710)
                     /gene="rpsA"
                     /locus_tag="VHI09_001622"
     CDS             complement(505..1710)
                     /gene="rpsA"
                     /locus_tag="VHI09_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEQ6121469.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
BASE COUNT          583 a          404 c          292 g          555 t
ORIGIN      
        1 agctagtttt tttaggtaaa actcttctta aattttaaaa cgtttcgaaa aatcaccaaa
       61 tgctttaata gcacttgcta aaaatggttt caccatctca tcaataatta accttgatta
      121 cttacaatct tatgaatctg agtcaaataa aattcaggct gtggcattac agagatattt
      181 aaaacctata ctcatcgttt tcaaggtcag aaaaataatg attttgaaaa gattcgtcaa
      241 ctcttctaga taattttgga caaattttgg aaaagaaaca aaaaaagcct atcaaataag
      301 gcttttttat atgatttcta tccaccttga gggaatcgaa cccccatctc aagaaccgga
      361 atcttacgtg atatccatta cactaaaggt ggtcacaact ttgtcaatac ccttatagta
      421 tatcataaaa tgaaataata tgaaataact tttctaacca gaagacaata aaaagttgag
      481 gaaaacctca actttttaga tttattacaa ttcaatatca ccgaaaagat cagccattga
      541 gaatccagtt tgtgtttctg ggagttcgta gtcacgtctt gattcacgtt ttggacgacg
      601 tgggcgtgat tgacgttttt cttctttgtt gtctccttca gcttgcgctg ggcgttcttc
      661 aagagctttg attgataatg atacgcgctc atcagctgca ttcacttcaa gaactttaac
      721 tgttacttct tgtcctacag aaagtacatc ttttggattt tcaacacgtt tgtgtgaaat
      781 ttgtgaaata tgaacaagtc catcaatacc tggtaatact tcaacaaaag caccgaagtc
      841 agtcaagcgt tttactttac cttcaacaac atcaccttga gcaagttttt gttcaacgcc
      901 gtcccatggt ccaggtgttg ttgctttaag tgaaagtgac acacgaccag cttcttcgtc
      961 aattgaaaga actttaactt caacttcttc accaactgaa acaactgatt taggtgacac
     1021 gttacgttcg tgagacaatt cagtcacgtg aacaagtccg tcaacaccac caaggtcaat
     1081 gaaagcacca aaacttgtta aacgcgctac agtacctgta acgactgcac cttctgaaat
     1141 cttagagaac acttcagcac gagcttcttt agcggcctct tcgataactt cacgacgtga
     1201 taggatgaaa cggttttctg ctgcatcaac ttctttgatt ttagcatcaa actcttgtcc
     1261 aacaaatttt tcagtgttgc gaacaaaacg agtgtcaatc attgaagcag ggataaatcc
     1321 acgaagtcct tcaaattcaa ctgaaaggcc acctttaaca gcacgggtac ctttaacagt
     1381 aacaacttcg ccttcacgac caacaagttt gtcccaggct ttacgagctt ccaaacgttt
     1441 tttagatact aggaaagtaa ctgtatcagt atctttacct actacttgac gaagaacaag
     1501 tacttcaact gtgtcgccag ctttaacaaa gtcgttaata tcagcatcgc ggtcgttagt
     1561 caattcacga agtgtcaaga caccttcaac acctgttcct tcgataacaa cgtttgcttg
     1621 accgttatca acagttaaaa cttccgcagt gacaacatca ccagggttca cttcgctaac
     1681 actgtttagc aaatcttcaa attcattcat tctaaaaaat cctccaacaa aagctagcca
     1741 atgctagctt ttgataacaa tatattttct caaggtaacc cgcaacgaca acaactctta
     1801 tctttgacgg tctcagacaa catctgatac ctct
//
LOCUS       DAUELZ010000153         1820 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.154, whole genome shotgun sequence.
ACCESSION   DAUELZ010000153 DAUELZ010000000
VERSION     DAUELZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.154"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..227
                     /locus_tag="VHI09_001623"
     CDS             <1..227
                     /locus_tag="VHI09_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEQ6121470.1"
                     /translation="NRIQSIHSSLAIEQNSLTVEQITAIIDGKRVLGNPREIREVKNA
                     YEAYEEILTLTPYDESHFLKMKEFQQYIYR"
     gene            251..1075
                     /locus_tag="VHI09_001624"
     CDS             251..1075
                     /locus_tag="VHI09_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine ligase"
                     /protein_id="HEQ6121471.1"
                     /translation="MGLGSDSLPVKISLFDVDTYLADSMQFHLEYILRLFPKSGVHYL
                     MPTFRGEAVDHRHLSQFYHSECEIEGDLHDVMNLVDRYIKFLVSEILRECRDSIFAIT
                     KDVSHLESFIDRKIPIITFEEACTILENEIDSLEYNDEHHFYVLTDVGEKKLMEHFEG
                     VVWVKNYPCKSVPFYQRFSTDHLFAYNADLLLGIGETVGCGERCETYEEVVENMNYLQ
                     VDASEYSWYLEMKKTRPMKSSGFGLGVERLIAWILKCDDVRKVQLISRENGIDYLP"
     gene            1089..1820
                     /locus_tag="VHI09_001625"
                     /pseudo
     CDS             1089..1820
                     /locus_tag="VHI09_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285438.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
BASE COUNT          655 a          218 c          364 g          583 t
ORIGIN      
        1 aaaatcgtat tcagtctatt cactcatcac tcgctattga acaaaattct ttaacagtag
       61 aacaaataac agccattatt gatgggaaaa gagtgttagg aaacccccgt gagataagag
      121 aagtaaaaaa cgcttatgaa gcttatgaag aaattttaac gctaactccc tatgatgaat
      181 cacatttttt aaaaatgaag gaatttcaac agtacattta ccggtaacaa catcgtcaat
      241 cacaagtcct atggggttgg ggagtgattc gctacctgta aaaatttcct tatttgatgt
      301 tgatacctat cttgcagatt cgatgcagtt tcacttagag tatatactta gactttttcc
      361 aaagtcagga gttcattact taatgcctac ttttagagga gaagcagtag atcatcgaca
      421 tttatcgcaa ttttatcata gtgagtgtga gatagaggga gatttacacg atgtgatgaa
      481 tttagtggat agatatatta agtttctggt tagtgaaatt cttagagagt gtcgagatag
      541 tatttttgct ataacaaaag atgtaagtca tttggaaagt tttattgatc gaaagatacc
      601 aattattacg tttgaagagg cttgtactat attggaaaat gaaatagatt cattggaata
      661 taatgatgaa catcattttt atgtgttaac agatgttggg gaaaagaaat taatggaaca
      721 ttttgaggga gtagtttggg taaaaaatta tccctgtaaa agtgtccctt tttatcaaag
      781 gttctcaaca gatcatctat ttgcctataa tgcagatttg ttgttaggaa taggcgaaac
      841 tgtaggttgt ggagaaagat gcgaaacata tgaagaagtt gtagaaaata tgaattattt
      901 acaagtagat gcttctgaat atagttggta tttagaaatg aaaaaaacaa gacctatgaa
      961 atcctcagga tttggtttag gagtagagcg tttaatagcg tggatattga agtgcgatga
     1021 tgttcgaaaa gttcaattaa tcagtagaga aaatgggatt gattatttac cataaaggag
     1081 gaagaaaaat gaaaaagaat attttaattg taggtggagg tccagagata actatatgtt
     1141 atgatttaat taacaatagt acggagtata atttcttttc agtatcagaa attcctacga
     1201 tgtatccaga gttgtttgta gaaacaattg aaaccagctt tagaaatttt ggaaagacga
     1261 ttgaacaaat taaagagctc aaatattcta ttgatgctgt tgcttgttgg gatgaagctt
     1321 taacgcatat agctgatgat atttcaaaag aattaggatt gaaccctatt ttatcgctag
     1381 attctcaatc attcaggttt aaagatcgaa tgagaatggt atgtgaagct ggtggactaa
     1441 aaatgcccaa atataaaatt atcaatcaat tttcagatac aaataaaata atcaattgga
     1501 aatatccatt aatagtgaaa ccaacctctt ttttagcaag tataggagtg aaaaaagttt
     1561 ataatttttc agaattacaa caagcagttt ctcaaatgct taatgtaaaa tttccggttt
     1621 acatagcttc aggggtatat gaattgggga attatataat ttagaaccta gagttttggt
     1681 ggaagagttt attgatggag aagaatatag cttagagagc gtggtaagaa atggaatcta
     1741 tacacctcta gggataacta aaaaatagtg gatgaaaaat tatttatgga tgaaataggc
     1801 catattttcc cttccaattt
//
LOCUS       DAUELZ010000154         1806 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.155, whole genome shotgun sequence.
ACCESSION   DAUELZ010000154 DAUELZ010000000
VERSION     DAUELZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.155"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..>1806)
                     /gene="lanM"
                     /locus_tag="VHI09_001626"
     CDS             complement(<1..>1806)
                     /gene="lanM"
                     /locus_tag="VHI09_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEQ6121472.1"
                     /translation="REENGISNFFDRYPYLLKQINREVGLVVESYSLLFDRFLKDLSE
                     IRSCFNITESLSNVEFSLGDSHSQKQTVVKIEFKGKSVYYKPKSYDSYNILLELISLL
                     KSNNIPSFSLPESLIKADYCWQLGVAYTSSNKDEVAKIYFKYGVLAAFSEIFSITDLH
                     MENVIVSGGDLYLIDVETFFQRKLNVQNQNFEGITVDTYQRIYKTSLSNGLFPVQFEK
                     NSAPNVSGISGKGGKRKKGKYELINKNRGDMKLVKTDYFQEDGYNILTLNGKVVEPLD
                     YANEVIAGFRECYTFLMSQRAKVKKILEDFPKLKTRAIFRNTSDYGKFLQASTNPKYL
                     FSEKKRENLFSILHESKHIEQFIVVSEIKDLMNGDIPYFSMDTSGNVYNSLGTVIGNL
                     GETTSLFDNIVTLNDERMKFTCELLGIVLKKPIKHWEREKGKSYQFLSISSEHNFSEE
                     ILDSIRRIFIDADKNSFSSEEEITWLNIDITETEQWVISPQNITLYNGLIGNALGYLY
                     AYQILGEEQYLVSLNKILKTLETTKNLIETSDMSVFLGKGGLIYLYFSLWKRLKLPQY
                     QKLYLDIIKEFSSQSLEEQNIDYISGVSGLLVVLCN"
BASE COUNT          588 a          342 c          230 g          646 t
ORIGIN      
        1 gttgcacaga acaactaata aaccagacac tccagaaata taatctatat tttgctcctc
       61 tagtgactgg ctggaaaatt ctttgattat gtctaaatat aatttttggt attgaggtaa
      121 tttaagtctt ttccacaaac taaagtatag ataaatcaaa cctccttttc ccaaaaaaac
      181 tgacatatca ctagtttcta taagattctt tgtggtttcc aatgttttca atatcttatt
      241 taatgatact agatattgct cttcaccaag aatttgataa gcatataaat aacctaaggc
      301 attcccaatc aatccattat acaaggtaat gttctgagga gatattaccc attgctcagt
      361 ctctgtaata tcaatattga gccatgttat ctcctcctct gaagaaaagc tattcttatc
      421 tgcatcaata aatattcgtc taatgctgtc taagatttcc tcactaaaat tgtgttctga
      481 ggagattgat aaaaactgat aagatttgcc cttttccctc tcccaatgct taatcggttt
      541 tttcaagact attccaagca attcgcaagt gaacttcatt ctttcatcat tcagggtaac
      601 tatattatca aataatgagg ttgtctctcc tagatttcca attactgttc ctaaagaatt
      661 atatacattt ccactggtat ccattgaaaa gtaaggaata tcaccattca tcaaatcttt
      721 aatttcacta acaacaataa attgttcaat atgttttgac tcatgtagta tcgaaaataa
      781 attctccctc tttttttctg agaaaagata ttttggattc gtagaggctt gtaaaaattt
      841 tccatagtcg gaagtatttc tgaatatggc tctagttttc aattttggga aatcttctaa
      901 aattttttta acctttgcac gttgcgacat aagaaatgta tagcattctc taaaacctgc
      961 aattacctca ttagcatagt cgagaggttc caccactttc ccatttaatg tcagaatatt
     1021 ataaccatct tcctgaaaat agtcagtttt cactaacttc atatctccac gatttttatt
     1081 tatcagttca tactttccct tttttctttt cccacctttt ccacttatcc cagaaacgtt
     1141 aggagcgctg tttttctcaa attgtacagg aaataaacca tttgaaagtg atgtcttata
     1201 aattctctga taggtgtcta cagtaatccc ttcaaaattc tgattttgta cattcaactt
     1261 tctttgaaaa aaggtttcaa cgtcaattaa atataaatct ccgcctgaaa caatcacatt
     1321 ttccatatgt aagtccgtaa tagaaaatat ttcagaaaat gctgctaaaa caccatactt
     1381 aaagtaaatt ttcgctactt catctttatt ggaacttgta taagctacac caagttgcca
     1441 acaataatcc gctttaatta agctttcagg aagactgaac gaaggtatat tattagattt
     1501 caatagacta ataagttcca acaagatgtt gtatgaatca tagcttttag gcttgtagta
     1561 aacggatttc cctttaaatt caatcttaac gactgtttgt ttttgagaat gactgtctcc
     1621 aagtgaaaat tcaacattac ttaaggactc agtaatatta aagcatgacc ttatttcaga
     1681 caaatctttt aaaaacctat caaatagcaa agagtatgat tccactacca atccaacttc
     1741 tctattgatt tgttttagca aatatggata tctgtcaaaa aaattactta tcccattttc
     1801 ttccct
//
LOCUS       DAUELZ010000155         1751 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.156, whole genome shotgun sequence.
ACCESSION   DAUELZ010000155 DAUELZ010000000
VERSION     DAUELZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.156"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..>1751
                     /locus_tag="VHI09_001627"
     CDS             <1..>1751
                     /locus_tag="VHI09_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEQ6121473.1"
                     /translation="FLKTNWLNISHHITLLVFGFYFSFYSLAKELVSSTAQPVNYYTH
                     LLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLNDKRFD
                     IWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLGYQGNTFSVALIL
                     AVVPISDIHLLKLISSRFSELVRAGNSQIALLKISGLLIVLLVIFATIIYVVLNALKH
                     LKSNKPSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAITLVAL
                     LAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVTSFVKK
                     SVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLVIVSCSMLIPFSYEK
                     EGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNYSQETI
                     ASIAQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPNIKAIK
                     NSTTAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHTISEFY
                     QGKNRIAMHPASANNFN"
BASE COUNT          555 a          263 c          311 g          622 t
ORIGIN      
        1 tttttaaaaa ccaattggtt aaatattagt caccatatta cccttcttgt tttcggtttt
       61 tatttcagtt tttatagttt ggcgaaagaa ctagtaagct ccacggcaca accggtaaac
      121 tattatactc atttactaaa tgtttctttt gtgggatata ttatatcact gattggatta
      181 tcttattatt tgagtcgcca agttagtcga cagttgtttt tgaaaactag ttttattgtg
      241 atatcttatc taattgtcag ctattgggta caaataacac agcacctgaa tgataaacgg
      301 tttgatatct ggtcattaac taaaaatcaa ttttatcaat ttcaagctct accttcttta
      361 ctcattattt tagtgatggc cactttaata aaaatattgg tagcatattt tgcaatagaa
      421 aaagatagat ttgggctatt aggctatcaa ggtaatactt tttctgtagc tctgatttta
      481 gcagttgtgc caattagtga catacatctg ttaaaactaa taagttctcg attttctgaa
      541 ttagtaagag caggtaatag ccaaattgca ctgttaaaaa taagtggact gttgatagtt
      601 ttacttgtca tatttgcaac aatcatatac gtggttttaa atgctctaaa acaccttaag
      661 tcaaataaac cttcattttc agtagcagct actactagtt tgtttttagc attagttttt
      721 aactatacgt tccagtatgg agtaaaaggt gatgaagcat tgctaggata ttatgttttc
      781 cctggagcta ctctttttca gatagtagct attacactag ttgctctttt agcatacgtg
      841 ataacgaata gatattggcc aactaccttc tttttgctta ttctgggaac aattatttct
      901 gttgttaatg atttaaaaga atcaatgaga agcgagccgt tattagtaac tgattttgtt
      961 tggttacaag aattaggttt agtgacaagc tttgttaaaa aatcggtgat tgtagaaatg
     1021 gttgtaggac ttgctatttg tattgtggtg gcttggtatc tacatggccg agttttagca
     1081 gggaaattat ttatgagccc tgtcaaacgg gcaagtgctg tattaggttt agttattgta
     1141 tcctgtagta tgttaatacc attttcttat gaaaaagaag gtaaaatatt atctggtctt
     1201 ccgattattt cggctttaaa taatgataat gatataaact ggttaggttt ttcaacaaat
     1261 gctaggtaca aatctttagc atatgtttgg acaagacagg tgaccaagaa aataatggaa
     1321 aaaccgacaa attatagcca agaaacaata gcgagtatcg ctcagaagta ccaaaaatta
     1381 gcagaagata ttaataaaga cagaaaaaat aatattgctg accaaacggt tatttatctt
     1441 ttaagtgaaa gtttgtcaga tcctgataga gtatcaaatg ttactgttag ccacgatgtt
     1501 ttacctaata tcaaggcaat caaaaatagc acaactgctg gactcatgca gtcagactcc
     1561 tacgggggtg gaactgctaa catggagttt caaacgttaa caagcttacc tttttataat
     1621 ttttcttctt cagtatctgt tctttattca gaagtctttc ctaaaatggc caaacctcat
     1681 acgattagtg agttttacca aggaaaaaat cgtattgcga tgcatcctgc tagtgctaac
     1741 aattttaata g
//
LOCUS       DAUELZ010000156         1722 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.157, whole genome shotgun sequence.
ACCESSION   DAUELZ010000156 DAUELZ010000000
VERSION     DAUELZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.157"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            83..703
                     /locus_tag="VHI09_001628"
                     /pseudo
     CDS             83..703
                     /locus_tag="VHI09_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            883..1193
                     /locus_tag="VHI09_001629"
                     /pseudo
     CDS             883..1193
                     /locus_tag="VHI09_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            1191..1722
                     /locus_tag="VHI09_001630"
                     /pseudo
     CDS             1191..1722
                     /locus_tag="VHI09_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
BASE COUNT          565 a          271 c          384 g          502 t
ORIGIN      
        1 ttttaataag tgttctcgtt tttttatgtt caggaggaaa atcatgcaaa tgaatgaaca
       61 gtcatcacga caaatggtga ttgccatcta tctcatttat tccaactcaa aatgatagta
      121 ctccttggct gttccaagtc gaaattgtta gtgattaaga aaacctacga gactatggtt
      181 tgatcgatga aggccagcag tctcccagtg aaaaagggcg tttggctaat aagatttatt
      241 taggaaagtt ggaacatgat tccaccccag tcttaagttc agacgggggt agtctaaaaa
      301 aacacgggac cagtctcaaa aacagacaag ccccgtgtta aattcagccc ctagtgagac
      361 tgaaaagagt gaaactgaag gtagtgaaac taaagagagt aatttagtca ttgaggacga
      421 ggaggaaaga aaggagtgca ctagtgtgaa aaagactgag ggtcacttta cccgtcaagt
      481 tgatcaggtc accaaatatg acaaagatta tatttggagt ttggttcatt cccaattacg
      541 tgaaggtggt ttatcacaag ctgctagtga cttagtcatg agctattttg aagaacgcta
      601 tgcttatgcg ctggaacaca tacggttcgc aagaacagct gaagctattg cggaatatgt
      661 ctttaatggt gtcttatccg aatggaccaa gcaactaagg tgacaagaag cgaagtgagg
      721 tgagtaagat gttttggtgg ctattattag gtgtttgggg tctaaggaca atttggttga
      781 ttattgaagt tatcactgca ccagaaatgg aagattatag ttgagtaagg acgttacata
      841 agcagtaagg tccttttatt ttgtcagaaa ggaatgagag atatgaagtt tttagatttg
      901 tttgcaggaa ttggaggctt tcgattgggt ttaattaatc agtgccatga gtgtattggc
      961 ttttgtgaga ttgataagtt tgcgaggcaa tcttacaaag ccatctatga gaccgaagga
     1021 gaaatagaat ttcatgacat tagacaagtc acagaccaag actttagaca acttagaggg
     1081 caagtggata tcatctgcgg tggtttttcc cttgtcaagc attttcactc gcaggcagac
     1141 gactgggatt tgagggcact aggagaactc tcttctttga gattactcga gcgactcata
     1201 cagacaaaga tcatattcat aatcacatct taatcaacgc cattgactgt aattcagata
     1261 aaaagttgat atggaattat gccttggaac gaaaccttcg tatgatttca gaccgcattt
     1321 ctaaaatggc tggggcgaaa attattgaaa agcatttctc ttaccgtgac tatcaaaaat
     1381 atagagcgac tagccataag tttgaactaa agcaacgtct ttattttttg atgcagcagt
     1441 caaagttctt tgatgatttt ttagaaaaag cagagtaatt acatgttcat attgatttta
     1501 gtcagaagca tagcagattc atgatgacgg atcgagtaat gaaaaaacca attcgaggtc
     1561 gtcaactcag taaacgagat ttatatgatg aagaattttt tcgtacacat tttgccaaga
     1621 tagaaattga aagtcgatta aaatttttgt ttaaccgtgc caattcatta gaggagctac
     1681 tattaaaagc aaaagaagtg aatctaacca ttgacttaaa ac
//
LOCUS       DAUELZ010000157         1709 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.158, whole genome shotgun sequence.
ACCESSION   DAUELZ010000157 DAUELZ010000000
VERSION     DAUELZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.158"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            1010..1318
                     /locus_tag="VHI09_001631"
     CDS             1010..1318
                     /locus_tag="VHI09_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ6121474.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSDVPYCLLSGCAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST
                     "
     gene            1373..1585
                     /locus_tag="VHI09_001632"
     CDS             1373..1585
                     /locus_tag="VHI09_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ6121475.1"
                     /translation="MVAAIEAGNYNDGILTEMNNLLEDIFIAKRPFIQKIKEKTLQAG
                     AATVLMTGSGPTVFALCQTEKQTIIV"
BASE COUNT          491 a          209 c          422 g          587 t
ORIGIN      
        1 tatattactg ataaggtcta tttaatgcgc aagatttgtg gtatgatgaa tatgattgag
       61 tattgatgct ttatgggatt accggtaagg ttttgaaaaa ggtttgtcac tactaggtgc
      121 ttttttaact atagaaagac agttatggat tgatgtattg taactaatgt tggaggttaa
      181 tggtcgttga gttttgagat atttacactg ataatcgggt ggaaactggt cgggtatcgg
      241 gtaatggagt gtggttcttc gtttaagcaa ggagattatc gtttggtggt tcatatttgc
      301 ttgttaaatg ttagggatga gatgctgagt cagcaaggcc atgaagataa agctgactgg
      361 ctagagcctt gagatgtgac gtttggtgga agtccttttt ggtgagatgg cacagcatat
      421 agtaatatta gatcctgagg aagaattggg actttcatta ggcttgactg gggtatgacc
      481 tcactttaca attcatttcg atgatgggtt tgatgatacg tttttagttc aagactttgt
      541 agagttggag aatttggttc tttatgagga agaagtgcag gtaattcgtt gagccagtcg
      601 tgaagatatt ttaggtatga ttgatgaagg ttcgttcatt ccttatctga agtataaaat
      661 aaatttgtgc tttgagatgg ttggtcaata taaagtccat caatcttaga agtaataatt
      721 tttgaatttt ttctgaggtt tgtgtatatt tgatagtgta ctgatgtaag aagtaaggag
      781 tactatgatg gcaatcaatg agagagcttc tgcaaaaatc aagttaggcc ttgatactaa
      841 gaataagcga tgagatggtt accacgattt atcgatggtc atgatgagta ttgatctgtg
      901 tgactatgtg acggttgacc atatagatga caataagatt gtctttgcct caaactgtcc
      961 gaaaatacct attaactaag ataatgatgt ttataagata gttcagctaa tgaaacatcg
     1021 atttcaagtt aaaagaggag tttccgttta cttggaaaag cggattccta tgtgtgctgg
     1081 tatgggagga ggttctagcg atgctgttac tatcagagca ttaaatcagt tatggctcct
     1141 tactcttagc cgaaaagata tgatggatat tggtataccg attggtagtg atgtacctta
     1201 ttgcttgctt tcaggttgtg cccaagtcac tggtaaaggt gaagtggttt gtcgcatttt
     1261 ggggctgtta tcgtcttggg tggttttggt taaaccagat tttggtatct cgacttgaac
     1321 aatttttcta gatattaatt gtaaaaccat ttcaagagtg tctactactc atttggttgc
     1381 ggcgattgaa gcgggtaact ataatgatgg gatattgact gaaatgaaca atttgctgga
     1441 agatattttc attgccaagc gaccctttat tcaaaaaata aaagagaaaa cactacaagc
     1501 tggtgctgct actgttttga tgacaggaag tggaccgact gtttttgctt tgtgccaaac
     1561 cgaaaaacaa actataatag tttaaaaggc ctttgcaagg aagtttttaa ggtgagaatg
     1621 ttgtaatatt ttgttgggat tgcttctttg gaagcttcat gttataatta actggttaat
     1681 gaaagaggag atttatgggg actttagaa
//
LOCUS       DAUELZ010000158         1706 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.159, whole genome shotgun sequence.
ACCESSION   DAUELZ010000158 DAUELZ010000000
VERSION     DAUELZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.159"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            196..1677
                     /gene="guaB"
                     /locus_tag="VHI09_001633"
     CDS             196..1677
                     /gene="guaB"
                     /locus_tag="VHI09_001633"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEQ6121476.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     KYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
BASE COUNT          494 a          290 c          386 g          536 t
ORIGIN      
        1 atttttgatt aaaaataaga cccaaatgaa ttttttgatt aataaaaaac ggataatact
       61 gatatattat gctgatagtg acttcagctg taggaatatt ttattgacaa atgattttag
      121 cgtgatatga tagtaacaat aaaaaataga ctagggtaat attccctaaa aataaaagaa
      181 aagaggatta tctggatgtc aaattgggac actaaatttt tgaaaaaagg ctacactttt
      241 gacgatgtct tgcttattcc ggcagagagt catgttcttc caaatgaagt tgatttaaag
      301 acaaagctcg cagacaattt gacattaaat attcctatta ttacagcagc tatggatacg
      361 gttactggta gtaaaatggc tattgcaatt gcgcgtgcgg gtggtttagg tgttattcat
      421 aaaaacatgt ctattactga gcaagcagaa gaagttcgca aggtaaaacg ttctgaaaat
      481 ggtgtcatca ttgatccatt cttcttaaca cctgaacaca aggtttcaga agctgaagaa
      541 ttaatgcagc gctaccgtat cagtggtgtg ccaattgttg aaactttagc aaaccgtaag
      601 ttggtcggta tcattactaa tcgtgatatg cgttttattt ctgattacaa tgcaccaatc
      661 tctgaacata tgactagtga gcacctcgtt acggctgctg ttggtacaga tcttgaaact
      721 gcagaacgta ttcttcatga acaccgtatt gaaaaacttc ctttggttga taatagcggt
      781 cgtctgtctg gtttaatcac tatcaaagat attgaaaaag taatcgaatt cccacatgct
      841 gctaaagatg aatttggtcg tcttctggtc gctgctgcgg taggtgttac ctcagatact
      901 tttgagcgtg ccgaggcctt gtttgaagct ggtgcagatg cgattgttat tgacacagct
      961 catggtcatt cggctggtgt tcttcgtaaa attgctgaga ttcgtgctca tttcccaaat
     1021 cgtactttga ttgctggaaa tattgccact gctgaaggtg cgcgtgctct ttatgatgca
     1081 ggtgtcgatg ttgttaaagt gggtattggt cccggatcta tctgtaccac tcgtgtggtt
     1141 gcaggtgtcg gtgttcctca agtgactgct atttacgatg ctgctgctgt tgctcgtaaa
     1201 tatggcaaaa cgattattgc tgatggtggt atcaagtatt ctggcgatat tgttaaggct
     1261 cttgcagccg gcggtaatgc tgttatgctt ggatcaatgt ttgcaggtac tgatgaagct
     1321 ccaggtgaaa ctgaaatcta ccaaggccgt aagttcaaaa cttatcgtgg aatgggttcc
     1381 attgcagcta tgaagaaagg atcaagcgat cgttacttcc aaggttccgt aaatgaagct
     1441 aataaactag ttccagaagg tattgaaggc cgtgttgctt ataaaggagc tgcttcagat
     1501 attgtcttcc aaatgttagg tggtatccgc tcaggtatgg gctatgttgg cgcaggtgac
     1561 attcaagaat tacatgaaaa tgctcaattt gttgaaatgt caggtgcagg cttgattgaa
     1621 agtcatccac atgatgtcca aatcacaaat gaagcaccaa actattctgt gcactgataa
     1681 tttaatttag taactttaag ggctga
//
LOCUS       DAUELZ010000159         1688 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.160, whole genome shotgun sequence.
ACCESSION   DAUELZ010000159 DAUELZ010000000
VERSION     DAUELZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.160"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            421..>1688
                     /gene="purB"
                     /locus_tag="VHI09_001634"
     CDS             421..>1688
                     /gene="purB"
                     /locus_tag="VHI09_001634"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEQ6121477.1"
                     /translation="MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIRKKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEQYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIGELFNPIYYTKRVD"
BASE COUNT          507 a          327 c          394 g          460 t
ORIGIN      
        1 ttaactggaa ataaatagat ttgtttgaat ttagttgttt atgcaaattt aaaaatgtat
       61 ctttcggaaa gttcggaaga aattagcata ctcattacta agtgctaaag caattgaaat
      121 aataaataga gagaggaata gggatatcgt attctcttaa ttgaatacgg actactaact
      181 tccttaattc acaaaatttt aagaaatata agaaaggagt gaggatgtta ccgagtattt
      241 gaactcgggc taacttatct cctcaataca catcaaacaa cagaaaggaa atgggcacta
      301 gtttcactaa actgaacccg agcggaaagc tcggaattta gataaaccgc ctaggacgca
      361 agcgtccatt gtcggttttc taaaattcag tcgctttctg gtcgcccttg gtatcttaaa
      421 atgatcgaac gttattcacg ccctgagatg gcggcaattt ggacagagga aaataaatac
      481 cgtgcttggt tggaggttga gattctggct gacgaggcat gggcagagtt gggtgagatt
      541 cctaaggagg atgtggctaa gattcgtaag aaggcggatt ttgacattga ccgtattctt
      601 gagattgagc aggacacgcg tcacgatgtg gtggctttca cgcgtgcggt ttctgagacg
      661 cttggtgagg agcgcaagtg ggtgcactac ggtttgacgt caactgacgt ggtggacact
      721 gcctacggtt acctctacaa gcaagctaac gacattattc gtcgcgatct tgagaatttc
      781 accaatatcg tggcagacaa ggcgcgtgag cacaaaatga ccatcatgat gggtcgtacc
      841 cacggtgttc atgccgagcc aacgactttc ggtctcaagt tggcgacttg gtacagcgag
      901 atgaaacgta atattgagcg ttttgaacat gctgccgcag gtgtggaagc tggtaagatt
      961 tcaggtgccg ttggtaactt tgctaatatc cctccatttg tggaacaata tgtctgtgac
     1021 aaattaggca ttcgtccaca agagatttca acacaagttc ttccacgtga cctccacgca
     1081 gaatattttg cagtgcttgc aagtattgca acttctatcg aacgtatggc gacagagatt
     1141 cgaggtctgc aaaagtcaga acaacgtgaa gttgaagaat tctttgccaa aggtcagaaa
     1201 ggtagctctg ctatgcctca caaacgcaac ccaatcggtt cagagaacat gacagggcta
     1261 gcgcgcgtga ttcgtggtca catggtgaca gcttatgaga acgtgtcact ttggcatgag
     1321 cgtgatattt cacactcatc agctgagcgt atcatcacac ctgacacaac tatcttgatt
     1381 gactacatgc tcaaccgctt tggcaatatc gttaagaact tgactgtctt cccggaaaat
     1441 atgatgcgca atatggaatc aacttttggt ttgatttata gtcaacgtgt tatgctcaaa
     1501 ttgattgaaa aaggaatgac acgagaagaa gcttatgact tagttcaacc taagacagct
     1561 tattcctggg acaatcaagt ggatttcaaa ccacttttag aagaagacac caaagttacc
     1621 tcttgtctta cacaagaaga aattggtgaa ctatttaatc cgatttatta cacaaaacgt
     1681 gttgatga
//
LOCUS       DAUELZ010000160         1684 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.161, whole genome shotgun sequence.
ACCESSION   DAUELZ010000160 DAUELZ010000000
VERSION     DAUELZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.161"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            5..>1684
                     /gene="cas9"
                     /locus_tag="VHI09_001635"
     CDS             5..>1684
                     /gene="cas9"
                     /locus_tag="VHI09_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEQ6121478.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASRVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDATLLSDILRVNSEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVT"
BASE COUNT          573 a          270 c          340 g          501 t
ORIGIN      
        1 aaaaatggat aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc
       61 ggtgatcact gatgattata aggttccgtc taaaaagttc aaggttctgg gaaatacaga
      121 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc
      181 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat
      241 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca
      301 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt
      361 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg
      421 aaaaaaattg gcagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc
      481 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag
      541 tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa
      601 ccctattaac gcaagtagag tagatgctaa agcgattctt tctgcacgat tgagtaaatc
      661 aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gattgtttgg
      721 gaatctcatt gctttgtcat tgggattgac ccctaatttt aaatcaaatt ttgatttggc
      781 agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt
      841 ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc
      901 tactttactt tcagatatcc taagagtaaa tagtgaaata actaaggctc ccctatcagc
      961 ttcaatgatt aagcgctacg atgaacatca tcaagacttg actcttttaa aagctttagt
     1021 tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata
     1081 tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat
     1141 tttagaaaaa atggatggta ctgaggaatt attggcgaaa ctaaatcgtg aagacttgct
     1201 gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct
     1261 gcatgctatt ctgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa
     1321 gattgaaaaa atcttgacct ttcgaattcc atattatgtt ggcccattgg cgcgtggaaa
     1381 tagtcgcttt gcatggatga ctcggaagtc tgaagaaacg attactccat ggaattttga
     1441 agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga
     1501 taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac
     1561 ggtttataac gaattgacaa aggtcaaata tgttactgag ggaatgcgaa aaccagcatt
     1621 tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt
     1681 aacc
//
LOCUS       DAUELZ010000161         1678 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.162, whole genome shotgun sequence.
ACCESSION   DAUELZ010000161 DAUELZ010000000
VERSION     DAUELZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.162"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            622..1233
                     /gene="rpsD"
                     /locus_tag="VHI09_001636"
     CDS             622..1233
                     /gene="rpsD"
                     /locus_tag="VHI09_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEQ6121479.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1388..>1678)
                     /locus_tag="VHI09_001637"
     CDS             complement(1388..>1678)
                     /locus_tag="VHI09_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEQ6121480.1"
                     /translation="LEVFEFLQREQLLSSLLSANGTKEIQAFIINKVRLLITTDLQDK
                     FRTEELSQTEKEYRSIYLAHAFFGVCQSWIAKGKKESPQEMTQFVLKMLTST"
BASE COUNT          540 a          299 c          307 g          532 t
ORIGIN      
        1 gataatattt ttaggttaga ttgaggtaaa aaagaggtct tcaccatggt taagcaaatg
       61 atcttagtac taaggttaaa agattatcgt gaaataaagt tttgacacaa gtgtaattaa
      121 tagaggaact tgaagtatcc gataaaacta tcttgaagtg aaaaaacgag taaaactccc
      181 ctgataaata caatatacta gccatttcag agcaattaag agtttccagt gaagtgcttt
      241 tggtagagag ctaagaaaaa acagtcaagg tttaagaact gcaaattagc atttaaatta
      301 ttttttgtta atgacagttg tctttattac cataactata acttcttata atatagtctt
      361 cttattcatc agtggtggtt cgtctcatat ctgaccttaa aatattaaac tgatcatcag
      421 atgatagata gactgacttt acaaaacaac tttcttatgg taaaatgttc tttgtgcgaa
      481 ataacagcag aaaaatatga agctcgtcaa caggtggtta tctattcaag taacctttgc
      541 tgtttaggcg aaaaccccct gcacgaatca ggatttttta aaccgttatt tcctcaaaaa
      601 atttagagga ggacatgtaa tatgtcacgt tatactggtc catcatggaa acaatcacgt
      661 cgccttggtt tatcacttac aggcacaggt aaagaattgg cacgtcgtaa ctacgttcct
      721 ggtcaacacg gtccaaacaa ccgtagcaaa ctctcagaat atggtttgca gttagctgag
      781 aaacaaaaac ttcgtttctc atacggttta ggtgaaaaac aattccgtaa cttgttcgtt
      841 caagctacaa aaatcaaaga aggaactctt ggtttcaact ttatggttct tttagaacgt
      901 cgtcttgaca acgttgttta ccgtctaggt ttagcaacta ctcgtcgtca agcacgtcaa
      961 ttcgttaacc acggtcacat ccttgttgac ggtaaacgcg ttgatatccc ttcataccgc
     1021 gttgatccag gtcaagtgat ttcagttcgc gaaaaatcaa tgaaagtacc tgcaatcctt
     1081 gaagctgttg aagctactct tggacgtcca gctttcgtat catttgatgc tgaaaaactt
     1141 gaaggttcat tgactcgcct tccagaacgc gatgaaatta acccagaaat caatgaagca
     1201 cttgtcgttg aattctataa caaaatgctt taattttaag attaaactta caaaaagcct
     1261 atttaacagg ctttttttgt atctcataac aaatttatcc tgatcccttc attttactat
     1321 ggcttctaaa aaaagttatc actaaggctg atgacttgta aaatgatcct ttcgtaaggg
     1381 ttactattta ggtactcgta agcattttga gaacgaattg tgtcatttct tgaggtgatt
     1441 cttttttacc tttagcaatc caactttgac agacaccaaa aaaagcatgg gctaagtaga
     1501 tacttcggta ttctttttcc gtttgagata actcttcggt gcggaacttg tcctgtaaat
     1561 cagtagtgat gagtaggcga actttattga tgataaaggc ctgtatttct ttggtaccgt
     1621 tagcagacaa aagggaagag agaagctgtt ctctttggag aaattcaaat acttcaag
//
LOCUS       DAUELZ010000162         1671 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.163, whole genome shotgun sequence.
ACCESSION   DAUELZ010000162 DAUELZ010000000
VERSION     DAUELZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.163"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(74..379)
                     /locus_tag="VHI09_001638"
     CDS             complement(74..379)
                     /locus_tag="VHI09_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEQ6121481.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            complement(391..810)
                     /gene="ruvX"
                     /locus_tag="VHI09_001639"
     CDS             complement(391..810)
                     /gene="ruvX"
                     /locus_tag="VHI09_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEQ6121482.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            complement(807..1076)
                     /locus_tag="VHI09_001640"
     CDS             complement(807..1076)
                     /locus_tag="VHI09_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEQ6121483.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            complement(1190..1588)
                     /gene="spx"
                     /locus_tag="VHI09_001641"
     CDS             complement(1190..1588)
                     /gene="spx"
                     /locus_tag="VHI09_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEQ6121484.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
BASE COUNT          501 a          339 c          245 g          586 t
ORIGIN      
        1 gcttatttat tcgaaaataa cattaaaaaa aatagccctc acggctactt tttaacatag
       61 tgtcactaac aatttagttc tcatctagaa aactattaaa tacctcttca atcatatccc
      121 attcagcatc tgaatcctca ggaatcggtt gtaaatcccc ttcagtacca tcttcatttt
      181 cagtaaatga ataggcttgg atttcgattt caccagactc atcttcttca gatcccgctg
      241 gaaccaagag aacataattt ttaccaaact cttcacgacc atcaattgtt aataaaattt
      301 caaacaaggt ttcatttcct tgttcatcca caagtgtgat aacttcgtgt tggtggtcat
      361 tttcatgatt atgtgtcatt ttttatctcc ttaaaaattc cgatttaaat agttctgtaa
      421 aatgagttgt gcagctaatt tatcaatgac tttttttcgc ttaccacgac taatgtcagc
      481 ttgctcaata agcatacgct ctgcctcaac agtcgtcaat cgttcatctt gataatggac
      541 tggaagtcca aaaaggtgct ctatgtggtt tccataagtt atacttgcat caacacgtgg
      601 accattggtg ttattcatat tttttggcaa tccaataaca aactgttcaa cttggtactg
      661 cttaacaagc tcttctaaac gtgtaaagcc aaattctgct ttttcctcat caatcttgat
      721 aatttcaagt ccttgggctg taaagccaag aggatcacta atagctacgc caaccgtttt
      781 cgagccaaca tcaagtccca ttattctcat ttaacatcaa ttccatttcc ttgtaaatag
      841 tagcgaacaa gttcttctac aatttcatca cgttcatatt tacgaatctg atttcgagca
      901 tcattataac gaggaacata agctgggtcc ccactcaaga cataaccaac aatttgatta
      961 attggattat aacctttttc atcaagtgaa tgatagactg ctgttaatgt ttcactaatt
     1021 tgtcgtttgt caccatcatc caacttaaag cggactgttt catctgtaaa tcccatactt
     1081 acaccttctt tctaaaatta agtgctatgc tttattatag cttatttcta ttaaataaac
     1141 aagaatttca agcgaagaag gtatttaaaa aaaagctcta caagagcttt tagagtgcag
     1201 cacgtaatcg tgcttcagta ttttcaatat tacgaataga gcgaggtaaa aaagcacgaa
     1261 tatcgtcttc tttgtagcct acttgcagac gtttatcatc aatcaaaatg ggacttttaa
     1321 ggatgcgcgg gttatcttga atcaaatcaa taacctcact aacacttaat tcttcaatat
     1381 cgcaatcgag agctttggca taacgatttt tagatgaaac aatgctctcc actccatttt
     1441 cggtttttga taaaattgct aaaatttctt ctttagttag cggttctttt cctaagttct
     1501 gttctttata agcaagctta tgggcattta gccaagtttt ggctttcttg cagctcgtac
     1561 aacttgaaat cgtgtaaatt ttaatcatgt tgctgactca ctttctattt cgtgatagct
     1621 ttattgtagc acagtttctt atctataaat ataggtcttt ggacctattt t
//
LOCUS       DAUELZ010000163         1670 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.164, whole genome shotgun sequence.
ACCESSION   DAUELZ010000163 DAUELZ010000000
VERSION     DAUELZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.164"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            88..729
                     /locus_tag="VHI09_001642"
     CDS             88..729
                     /locus_tag="VHI09_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEQ6121485.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGRVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWESIQPNEEVDKVFTVPLRQLLVTDPVYYHLEATPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWGMTAQFTKCLTDILNDESIAKKNPLDIEKSKGR"
     gene            complement(776..1618)
                     /locus_tag="VHI09_001643"
     CDS             complement(776..1618)
                     /locus_tag="VHI09_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEQ6121486.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVIVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKVDWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMAVVLFALGIWLGLWMSQLE
                     EKYK"
BASE COUNT          593 a          298 c          333 g          446 t
ORIGIN      
        1 cactaactag gagtaataag ttgaccaaaa agctaggatt ggttatgatg aagataagat
       61 taacggaaag agaaattagg gaaggctatg aaggacttat taaaacagta tcaggcaaaa
      121 ccactaggag aggagaagcg gtacgccgta tttttacctc ttattcttgt caatgatgac
      181 tggcatgtct tatatgaagt cagaagtcag catatttcac aaccggggga agtttctttc
      241 ccagggggac gagtagaaaa tcaggagaca cttcaagaag ctgctatccg agaaacagtt
      301 gaggaattga ctgttgatgc gtcccagatc cagctatggg gtgaaattga ttatttagta
      361 cagtcttcta gaactattca ttgtttcgtt ggtcaattag tcgttgatga ctgggaaagc
      421 atacagccta acgaagaagt tgacaaggtc tttacagtac ctttacgtca gctacttgtc
      481 actgatcctg tctattatca tttagaagcc actcctattg agacaacgga ttttcctttt
      541 gaccgtattc gtaatgggaa atactaccaa tttagtcaag aataccgtag tattccgttt
      601 tatgaaaatc tagaagaaac catatgggga atgactgccc agtttactaa gtgtttaacc
      661 gatattctaa atgatgaatc aattgcaaag aaaaacccct tagatattga aaaatctaag
      721 gggagataga gcactagttt gttggcgtca actttttgtc aacaacctgt aaaggttatt
      781 tgtatttttc ttctaattga ctcatccaaa gtccgagcca aataccaaga gcaaataaca
      841 caacggccat aagccaggtt aagatacctg catgactgta ggaaatggat tcgggactac
      901 tactatttgg aataagaatt aatagcgtgc tggataatac aataccaatg ataaagtggt
      961 aaacttttga atgatggtgc tgtaaagcat aatccatact tttagaaaaa gccaagatgg
     1021 ctaggactcc tccgatagca ataggtaaga aagtccctaa taaatccaaa gatttgaatc
     1081 ctattaacat aggaccatag agtcctaaga taagtaagag atttgacggg cttagtccag
     1141 ggaccagtac tcctaaagca attaaggcac ctgctaaaat aaaggttaaa aagttagctg
     1201 gtaaggtacc gattaagtca tttaagaagt aaagacctag tcctgaaata acaaaggtaa
     1261 ggactagcca tagccagtca actttgtctc tttgagactg cttggttgac tccttaatca
     1321 gactaggaat agtgccgaca atagcgccag caaatcccca taagacgata acttgatagt
     1381 gcttaagcaa aaactcaacg gggaaagaaa agagagcaat acctaaaata ccaccaattc
     1441 ctactggtaa gaaaaataag acattttcaa taaagttatc cctcatatga gctaaaaagg
     1501 aaatcattcg ctcatagatt cctaaaatgg ctgcgagaac accaccggat acaccaggta
     1561 aaataaaacc tagtgcaata atcatgcctt taaaaactct tgaaataaat gaaaccattg
     1621 ttgcctcctg aaaagaatga ttttattgta tcataaattt aaagaattgc
//
LOCUS       DAUELZ010000164         1604 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.165, whole genome shotgun sequence.
ACCESSION   DAUELZ010000164 DAUELZ010000000
VERSION     DAUELZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.165"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            111..1502
                     /locus_tag="VHI09_001644"
     CDS             111..1502
                     /locus_tag="VHI09_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ6121487.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLVFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAITIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPILAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLAYGYTHSIENK"
BASE COUNT          505 a          235 c          273 g          591 t
ORIGIN      
        1 agaagaacag aagatagcaa aagtttcagt caaccaataa gatgaaattt acaatcatta
       61 ctaactatga tacacttagt agaaaatcat ttactataga aggtgataac atgactattt
      121 ttcgtaaaaa aaagaaatat tccaataaaa cagaaatgca aaggcacttc aaagttattg
      181 atttagtttt tttaggccta ggttcaatgg ttggaacagg tatctttaca gttactggca
      241 taggcgctgc taagtatgca ggtcctgctt taacaatatc gattatcatt tccgcgatta
      301 ctattggaat tttagcctta ttttatgcag aatttgcgtc tcgtatgcct tctaacggtg
      361 gtgcttatag ttatgtttat gcaaccttgg gagagtttcc agcatggctt gttggctggt
      421 atattattat ggaattttta acagctatat ctagtgttgc tgttggctgg ggaagttatt
      481 taaaaggatt acttgccaat tataatatag tattaccaaa cgctttaaat ggcacgttca
      541 atcttaagaa tggaacttac atagatattc ttcctgtttt agtgatgttt ttcgtgacag
      601 ggattgtcct aatgaattcg aaattagctc tgagattcaa tagtttttta gttattttaa
      661 aattttcagc actggcttta tttatctttg taggcatttt ttttattgat cacaataatt
      721 ggtctcattt tgctccatat ggtgtaggac aaattactgg aggtaaaaca ggtatctttg
      781 ctggtgcttc tgttatgttc tttgcatttt taggatttga atctatttca atggcagttg
      841 atgaagtgaa agaaccacaa aaaacaattc ctaaaggtat cattttatct ttgattattg
      901 taacagcact ctatatagtt gtcaccacta ttttaacggg aattgtacac tacactaaat
      961 taaatgttcc agacgcggtg gcttttgctt taagaaatat tagactttat tgggcagctg
     1021 actatgtgtc gatagttgct attttgactc ttattacggt atgtatttct atgacttatg
     1081 cattagctag aacaatttac agtattagta gagatggttt attacctaaa tcactttata
     1141 ctctcactaa aaaaaataaa gtgcctcaaa atgctactct tgtcactgga ctacttgcta
     1201 tgatttgtgc gggaatattt cctttatcga gtctagcaga gtttgttaat atttgtactt
     1261 tagcatattt aattattctg tcgggtgcaa ttattaaact cagaagaata gaaggagaac
     1321 caaaagcaaa tgaatttaaa actcctctag taccattcct accaatattg gcaattatca
     1381 tttgtctatc atttatgagc caatataaag cttttacttg gattgctttt gctattgcca
     1441 caattattgg aacattaatc tacttagctt atggttatac tcattcaata gaaaataaat
     1501 aatgttttga taataagatt tgacattaca ttatagacta taatgtttag aagtaaatga
     1561 gtagtaaaat caatacctac ttacttatca aagttgtaat attt
//
LOCUS       DAUELZ010000165         1590 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.166, whole genome shotgun sequence.
ACCESSION   DAUELZ010000165 DAUELZ010000000
VERSION     DAUELZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.166"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..203
                     /locus_tag="VHI09_001645"
     CDS             <1..203
                     /locus_tag="VHI09_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEQ6121488.1"
                     /translation="VDFEKQNQQVVKLKKEYNELSKSTKKEKQLAERLKDDNFVKKYA
                     RAKYYLSREGEMIYPIPGLLPK"
     gene            338..>1590
                     /locus_tag="VHI09_001646"
     CDS             338..>1590
                     /locus_tag="VHI09_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEQ6121489.1"
                     /translation="MRKLLVAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQLPSNPNLYQETCAYKDSDLTLPAGRLGVNQPLPIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDNYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGK
                     ISKIADNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFRSEIKALSGIDWDME
                     QRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHD
                     VAIVYGNTPFILSIFTNKSTYEDITA"
BASE COUNT          608 a          240 c          251 g          491 t
ORIGIN      
        1 atgttgattt tgaaaagcaa aatcaacagg tggttaaatt aaaaaaagag tataatgaat
       61 tgtcaaagag tacaaaaaaa gaaaaacaat tagcagaacg actaaaagat gataattttg
      121 tcaaaaaata tgctagggca aaatactatt tatcgcgtga aggagaaatg atttatccta
      181 ttccaggact attaccaaaa tgatgatgga caatattgta aaaaaaatag aagcatttct
      241 tgctttttct gataaaaaat tagcagagct gcaacaggaa aatcaaaaag ttaaagaaga
      301 aagtcagtat attcaaaaaa actagaaagg agtcaatatg agaaaattat tagtggctat
      361 gttaatgact ttttttctga ctcctttacc agtgattagt acagaaaaaa aacttatatt
      421 ttcaaaaaat gctgtttatc aattgaaaca agatgtcgtt caatcaacac aattctataa
      481 tcaactaccc tctaatccaa atctttatca agaaacgtgt gcttataaag acagtgattt
      541 aactctacca gcaggaagat taggtgtaaa tcaaccatta cctattaaat cgcttgtgct
      601 taacaaagaa tctttaccgg tttttgagtt agctgatggt acctatgttg aggctaatcg
      661 acaattgatt tatgacgata ttgtacttaa tcaagtagat atagataact atttttggac
      721 acaaaagaaa cttaggcttt attcagcccc ttatgtttta ggtacgcaaa caattccttc
      781 ttctttttca tttgctcaaa aagttcatgc cactcaaatg gcacaaacaa accatggaac
      841 ttattatctt attgatgata agggctgggc atcacaagaa gatctagttc aatttgataa
      901 ccgcatgtta aaagtccagg aaatgctctt acaaaaatat aataacccaa attattcaat
      961 ttttgtaaag caactcaaca cacaaacaag tgctggtatt aatgctgata aaaaaatgta
     1021 tgctgcaagt atctcgaagt tagcaccact ttatattgtt caaaaacaat tacaaaagaa
     1081 gaaattagca gagaataaaa ctttgactta tactaaagat gttaatcatt tttatggaga
     1141 ctatgatcca ttgggaagtg gtaaaattag taaaatagct gataataaag attatcgtgt
     1201 tgaagaccta ctgaaagctg tagcacaaca atcggataat gtagcaacta atattttagg
     1261 ttattatcta tgtcatcagt atgataaagc tttccgctca gagataaaag ctttatcagg
     1321 tatcgattgg gatatggagc agcgattatt aacttctcgt tcagctgcaa atatgatgga
     1381 agctatttat catcaaaaag gccaaattat ttcttacctt tcaaataccg aatttgatca
     1441 acaacgtatc acaaaaaata ttactgttcc agttgcacat aaaattggtg atgcttatga
     1501 ttataaacat gacgttgcta ttgtttacgg taatactcca tttattttgt ctatttttac
     1561 aaataaatca acctatgaag atattacggc
//
LOCUS       DAUELZ010000166         1567 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.167, whole genome shotgun sequence.
ACCESSION   DAUELZ010000166 DAUELZ010000000
VERSION     DAUELZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.167"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..561)
                     /locus_tag="VHI09_001647"
     CDS             complement(<1..561)
                     /locus_tag="VHI09_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ6121490.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHKKILVDSVCLEDKIKHLINDPKCIGLNITLPYKREVMRYIDNLDRVAEIT
                     GSVNNIYKSGNIITGTNTDWKGIYNTLVFFNISHITKCCVLGTGGTARAAIFTLQKLQ
                     ISPTVVYREPISENTRSLIKSFPDLKY"
     gene            complement(630..701)
                     /gene="shp3"
                     /locus_tag="VHI09_001648"
     CDS             complement(630..701)
                     /gene="shp3"
                     /locus_tag="VHI09_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEQ6121491.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            781..>1567
                     /gene="rgg3"
                     /locus_tag="VHI09_001649"
     CDS             781..>1567
                     /gene="rgg3"
                     /locus_tag="VHI09_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEQ6121492.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     TLLDNELNFYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDI"
BASE COUNT          545 a          268 c          218 g          536 t
ORIGIN      
        1 atactttaaa tcagggaaac tctttatcaa acttcttgta ttctcactaa ttggctccct
       61 gtatacaact gttggtgaga tttgtagttt ttgtaaagtg aaaatagcag ctctagcagt
      121 accgccagtg cctaaaacac aacattttgt tatatggcta atattaaaga aaactaatgt
      181 attataaata cctttccaat cagtattagt tcctgtaatt atatttccag atttataaat
      241 attgttaaca gatccagtta tctctgctac tctgtctaaa ttgtcaatat acctcattac
      301 ctctctttta taaggtaaag ttatatttaa tccaatacat tttggatcat ttatcagatg
      361 tttgatttta tcttctaaac acactgagtc taccaaaatt tttttatgtt tatattcatc
      421 cagaactcct atgttgtgat ttagatattc aaataattca tctgatatag aatgttcaac
      481 tggattacca agcaatgcag aatacataaa tttcatcctc ctacgtttaa ttagtttact
      541 actaacataa agtacaagca ttacacagag ctaaccatta cctataagtc tttagagtat
      601 ctaaagactt aggtatacac ctaagtaact tacccaccaa caataatgat aatatccatt
      661 gctaaaatta aaatcggcaa aaattttgaa attttcttca taagtggact tcctttcagt
      721 ttttatttat tgtacaataa aaaaaaactg aattgatttt tgttgggaaa gtgggaaaat
      781 atgaaatcaa aacttggtgc cacacttaga aaagttcgaa aaggaaagca aattagtctg
      841 tgcttcgtgg cagatgaaca cctttcaaaa tcacaaatct ctcgttttga gcgcggggag
      901 tcagagatat cgtgtatccg actgatcaat attctagaca aattacacat tactttggat
      961 gagtttctta ttttacatga tgaagactat accaattccg aaacatttgc taacctggta
     1021 cagtatattc gtaagcaata ttcatcacaa aatatcaata atatagcatg tttactttcc
     1081 gacacttctg attacactct gaattctttt gagaaaacaa tggtaaagtc cattttacac
     1141 acaatggatt caagcatcat tccatcagat gatgaattac ttcaactaac agattatctc
     1201 tttaaagttg aaaaatgggg ctactatgaa atccttttgt tgggtaactg tgtacgaaca
     1261 attaactaca actcctactt cctattgaca aaggagatgt taaataatta catttactct
     1321 tcacggaaca aaactaataa acgaattgtc actcagcttg ctataaattg tttcattctt
     1381 agcatcaata aggaggaatt ttctaattgc tcctatttaa ttgatgagat aaaaaccttg
     1441 ttagataatg aattaaactt ctatgaacag acagtttttc tctatgcaac aggttacttt
     1501 gaatttaagc gccaattaga aactggagtt gaaaagatga aacaagccat acaagttctt
     1561 gatattt
//
LOCUS       DAUELZ010000167         1562 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.168, whole genome shotgun sequence.
ACCESSION   DAUELZ010000167 DAUELZ010000000
VERSION     DAUELZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.168"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..562
                     /locus_tag="VHI09_001650"
     CDS             <1..562
                     /locus_tag="VHI09_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEQ6121493.1"
                     /translation="SQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLTSYARL
                     LSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYFIWSNG
                     TNERKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVKAIQND
                     LKNIQYDVTIGKGYLLKHKTFFKISR"
     gene            741..1436
                     /locus_tag="VHI09_001651"
     CDS             741..1436
                     /locus_tag="VHI09_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ6121494.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYHYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            1438..>1562
                     /locus_tag="VHI09_001652"
     CDS             1438..>1562
                     /locus_tag="VHI09_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEQ6121495.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFILA"
BASE COUNT          527 a          228 c          286 g          521 t
ORIGIN      
        1 aagccaattt ttctcagtta ttacaatgca aaatcatatt ccttggtcat ccgattatcc
       61 tgaagaaatt gttgctgaag gaaaaaattt cacggaagaa gaaaatcaca acctaacaag
      121 ttatgctcgg ttattatcgt ttactgataa ggaaacaaga gcatttttag aaaaattaac
      181 acaaattaac aagcctatca cagtggtgtt ttacggagat catttacccg gtttatatcc
      241 tgatagtgct tttaacaagc atattgaaaa taaatacctt actgattatt ttatttggag
      301 taatggtact aacgagagaa aaaatcatcc gcttatcaac tcaagtgatt ttactgcagc
      361 tttatttgag catactgatt caaaagtatc accttactat gctttgttaa cagaggtact
      421 gaataaagct agtgtcgata aatcaccaga tagtcctgaa gttaaagcta ttcagaatga
      481 tttaaaaaat atccaatacg atgtgactat aggaaaaggt taccttttga aacacaaaac
      541 tttttttaag atatcacgtt aagaaaaatg ctaggctgtc cgagtataga cgagcatcaa
      601 ggtttggtaa tttgctcaaa tgattgtcaa cagagtgtct aggaccaggt ttaaaagatt
      661 ataattctta aggtcttatt cgtttataaa aaatgaatac tcttttaaaa tcttaattga
      721 aatgaatagg agaagttttc gtgaaaaagt taatcattat tcctgcttac aatgaaagca
      781 gtaatattgt caatactata cgtactattg aatcagatgc cccggatttt gactatatca
      841 ttattgatga ttgctcaacg gataatacgt tagcaatatg tcaaaaacag gggttcaatg
      901 ttatttcttt gcccattaac ctgggaattg gcggtgcggt gcaaactggc tatcattatg
      961 cacaaagatg tggatatgac gttgcagttc aagtagatgg agatggtcag cacaatccat
     1021 gctatttgga aaaaatggtt gaggtattag ttcaatcttc agtaaatatg gtaattggat
     1081 cacgatttat cacaaaagaa gggtttcagt catcatttgc tcgacgtatc ggtataaagt
     1141 attttacttg gcttattgcg ctactaacag gaaaaaaaat aacagatgca acgtcaggtc
     1201 taaggttaat tgaccgttca ttgattgaac gttttgctaa tcattatcca gatgattatc
     1261 ctgaacctga aacagttgtt gatgtattgg ttagtcattt taaagtgaaa gaaattcctg
     1321 ttgtaatgaa tgagcgacaa ggcggtgtgt catctatctc gttgacgaaa tcggtctatt
     1381 atatgattaa agtgacttta gctattttag ttgttagatt gaaaggaaat cgttaaaatg
     1441 acggtaacag ccagtttatt tataagtttt attgtattaa cctttgtttt ctttttgatt
     1501 aacttaatca aaaaagacaa actagcgatt aagtactctt tattgtggtt tattttagct
     1561 tt
//
LOCUS       DAUELZ010000168         1555 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.169, whole genome shotgun sequence.
ACCESSION   DAUELZ010000168 DAUELZ010000000
VERSION     DAUELZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.169"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..216
                     /locus_tag="VHI09_001653"
     CDS             <1..216
                     /locus_tag="VHI09_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEQ6121496.1"
                     /translation="KLRLQNKVTLMAILGAIFLLAQQLGIKLPSNIADIANTAVTLLV
                     LLGVVTDPTTKGLSDSEQALTYHEPKK"
     gene            332..1537
                     /locus_tag="VHI09_001654"
     CDS             332..1537
                     /locus_tag="VHI09_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEQ6121497.1"
                     /translation="MTFLDKIKQGCLDGWAKYKILPSLTAAQAILESGWGKHAPHNAL
                     FGIKADASWTGKSFNTKTQEEYQAGVITDIVDRFRAYDSWDESIADHGQFLVDNPRYQ
                     SVIGEADYKKACHAIKDAGYATASGYAELLIQIIEENNLQKWDKEAIVRKEKQMINSQ
                     CREVIEFFINLANAGMGVDKDSFAGWQCADVPCYAAKHWFDVDLWGNAIDLLDSAAAV
                     GWEVHRMPTDANPLTGAFFVQSVPYHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALY
                     VGAPARFNTRDFTGVIGWFYPPYQGDTVTQPVSTEPQTSETIVETAKTGTFTLDVAEI
                     NIRRWPSLASEVVGSYKQGDTVSFDNEGYANGYYWISYVGGSGMRNYLGIGQTDKDGN
                     RISLWGKLN"
BASE COUNT          455 a          300 c          379 g          421 t
ORIGIN      
        1 aaattacgac tacaaaacaa agtaaccttg atggctattt tgggagctat ctttttgtta
       61 gcgcaacaat taggtattaa attaccatca aacatcgcgg atattgccaa cacagctgta
      121 acgcttttgg tgttgcttgg cgttgtcaca gatcctacta caaaaggctt atctgacagc
      181 gaacaagcat tgacttacca tgagcccaaa aaataggagg agccatgcga gcaatcacac
      241 gattagcatt agttatagca atcgcaatac tgtatgtgcc attatctgtg gttgctctta
      301 tcttttatcc gtttttagat aaggaggaca gatgaccttt ttagataaaa ttaaacaagg
      361 ctgtttagat ggctgggcta agtacaaaat cttgccatcc ttgaccgcag cacaagctat
      421 cttagagagc gggtggggta aacatgcacc acataacgct ttgtttggta ttaaggcaga
      481 cgcaagctgg accggtaagt cgtttaacac caaaacccaa gaggaatatc aagctggtgt
      541 tattactgac attgttgaca ggtttagagc ctatgacagc tgggatgaat ctatcgctga
      601 ccatggacaa tttttagtag ataatccacg ctaccagtct gttattgggg aggctgatta
      661 taaaaaagct tgtcatgcta taaaagatgc aggctatgcg actgcgagtg gctatgcgga
      721 gctacttatc caaattattg aggagaataa cttgcagaaa tgggataaag aggctattgt
      781 cagaaaggag aagcaaatga ttaattctca atgtcgagaa gtcattgaat tttttattaa
      841 tttggccaat gctggtatgg gtgttgacaa agatagtttt gcgggctggc aatgtgcaga
      901 tgtgccttgt tatgcagcaa agcactggtt cgatgtcgac ctttggggaa atgcgattga
      961 cttactagat agcgctgctg ccgtaggttg ggaagtccat cgcatgccga cagatgcaaa
     1021 tccactgacg ggagcatttt ttgtccaatc agtgccgtat caccaatttg gacatacggg
     1081 aattgttatc gaggatagtg acggttacac catgcgcact gtcgagcaaa acattgatgg
     1141 caatcctgat gctttgtatg tcggtgcacc agctcgtttt aacactcgtg actttactgg
     1201 cgtgataggt tggttttacc caccatatca aggggataca gtcacgcaac cagtcagcac
     1261 cgagccgcaa acttctgaaa cgatcgtgga gacagcaaaa acaggcacct ttacccttga
     1321 tgttgcagag atcaatatca gacgctggcc aagcctagcc agtgaagtag taggtagcta
     1381 taagcaaggt gatactgtca gctttgacaa tgagggttat gccaacggtt actactggat
     1441 tagctatgtt ggcggctcag gtatgcgtaa ctacctaggt attggacaga ctgataaaga
     1501 tgggaatcgc atcagccttt ggggtaaatt aaattaggag gtaaagctcc gagat
//
LOCUS       DAUELZ010000169         1547 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.170, whole genome shotgun sequence.
ACCESSION   DAUELZ010000169 DAUELZ010000000
VERSION     DAUELZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.170"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            18..938
                     /locus_tag="VHI09_001655"
     CDS             18..938
                     /locus_tag="VHI09_001655"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEQ6121498.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            992..1240
                     /locus_tag="VHI09_001656"
     CDS             992..1240
                     /locus_tag="VHI09_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEQ6121499.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(1365..>1547)
                     /locus_tag="VHI09_001657"
     CDS             complement(1365..>1547)
                     /locus_tag="VHI09_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ6121500.1"
                     /translation="TDEEKKKIFDRFYRVDKARTRQTGGFGLGLALAQQIVMSLKGNI
                     TVKDNDPKGSIFEVKL"
BASE COUNT          542 a          292 c          224 g          489 t
ORIGIN      
        1 aatgattagg agctaccatg tcaaatgaat ttatcaactt tgaaaaaatt tctagagagt
       61 cttggaaaac attacatcaa aaagcaaaag ctctcctcac acaagaggaa cttaaaagta
      121 ttactagctt aaatgataac atcagtatta acgatgtcat tgatatctac cttccattaa
      181 ttaatctgat ccaggtttac aaaattgcgc aagaaaatct ttctttttct aaaagccttt
      241 ttctaaaaaa agacattcaa ttaaggcctt ttattattgg aatttctggt tcagtagctg
      301 ttggtaaatc aacaactagt cgtcttttac aacttttatt atcacgaacg caccccaata
      361 gtcaagtcga actggtaacg actgatggtt ttttatatcc taatcagttt cttattgaac
      421 aaggactgtt aaatcgtaaa ggatttccag aatcttataa catggaactt ctcttagatt
      481 ttctagattc catcaaaaat ggtcaaacgg cttttgcacc cgtctattct catgatattt
      541 acgatattat cccaaatcaa aaacaatcat ttaataaccc tgacttcctt atcgtcgaag
      601 gtattaatgt ttttcaaaat caacaaaaca atcgtttata tatgagtgat tactttgatt
      661 tttctattta tattgatgca gacagcagtc acatcgaaac ctggtacatt gaacggtttc
      721 taagcatttt aaaattagca aaacgtgacc ctcataatta ttatgcccaa tatgcacaat
      781 tacctcgatc tgaggctata gcatttgcgc gcaacgtttg gaaaaccgtt aatttagaaa
      841 atcttgaaaa attcattgaa cctacacgta accgtgctga attaatttta cataaaagtg
      901 ctgatcataa aattgatgaa atttacctca aaaagtgata aagggttgcc aaacgcatat
      961 attttctata taataatgta gttagattaa tttggaggtg aaaacattgg caaatattaa
     1021 atcagctatt aaacgcgctg aacttaacgt taaagcaaac gaaaaaaact cagcacaaaa
     1081 atcagctatg cgtactgcta tcaaagcatt tgaagctaac ccatctgaag agcttttccg
     1141 cgctgcttct tcaagcatcg ataaagctga atcaaaaggt ttgattcaca aaaacaaagc
     1201 aagccgcgat aaagcacgcc ttgctgcaaa acttggctaa taaatatcca atgaaaactc
     1261 cagctggagt ttttttatac ggtaaaaact caaaaaatac aataaagcgt tgacagttaa
     1321 aaataacttt aaaagggctg accttattgg tcgccctttt tagcttatag tttgacttca
     1381 aaaatactac ctttaggatc attatccttt actgtaatat ttccttttaa agacatcacg
     1441 atttgctgag ctaaagccaa ccccaagcca aatccacctg tttgccgtgt tctagctttg
     1501 tcaactcgat aaaaacgatc aaaaatcttt ttcttttctt catctgt
//
LOCUS       DAUELZ010000170         1540 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.171, whole genome shotgun sequence.
ACCESSION   DAUELZ010000170 DAUELZ010000000
VERSION     DAUELZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.171"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..1485)
                     /locus_tag="VHI09_001658"
     CDS             complement(<1..1485)
                     /locus_tag="VHI09_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEQ6121501.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDAFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTGFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMENVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKESHRKILEDELLENKAAYIVVNRYKNLPKIIQ"
BASE COUNT          584 a          275 c          246 g          435 t
ORIGIN      
        1 ttgaatgatt ttaggcaggt ttttatagcg attaacaacg atataagcag ccttattttc
       61 taatagttca tcttctaata tttttcgatg actctctttt tgcgtattga tgtcaggaga
      121 actaaattga gaaacagatt tagctttact gtccaaataa attttagaag tatcatccca
      181 aacataaata gattgagttt tatttagttt ctgttctaaa taactagcaa tatgatcacg
      241 ctcctgatta agaggaatac tactaatgaa acaataagta gaacagatta gtattgtccc
      301 tacaattaaa attggcaaat aaaagtgtct cttaagataa atcatcatta ctcgaccaac
      361 accattttta ccatgacgtc tgcgatgact actctgacct aatcctatac catactgata
      421 gccaacagga atagcagtca aaattaatcc aaaaggtaaa agaggtaaaa gatgataggg
      481 gcgatagtct tgtgaaaata tagccacaag tatagattct agaatgacca tgacaaatag
      541 ccacttgacg accctatcag aattgttttt aaatcgccta atgacattct ccattcccgt
      601 caaaagacca agccccaaag cgaagaacaa ctgaatagcc agtccaaaaa gcaacgataa
      661 gtttcctgat ttaaaaaaag taaaaggata aatcatcgtt tgtgataaat aaggatttag
      721 cacttgtaag ttcaaaatga aatatcctgc tgtgtaaaaa actaaaatca ttccaaaaat
      781 cgaagctagc agttgataaa aacctcttgc aagatgcttt tggcttatat tataagaaaa
      841 aactgtcaca caagcaaaag accaaaagat aagggtactc gggtcaatta gcattgcaag
      901 agcacctaca aagccaaaaa gaataaatgc ctcatctttc actaaacagg caaaatactt
      961 agttaaaaac caagccgata ttaatataaa tggcatagcc agttgagtag gatacaatcc
     1021 cccaaaacca agactaacag ataacaaata gtagctaatt gaaaaagtta aagccacttt
     1081 ttgaaagcct gtcatataat taatgagttt ataaagatat aaaccagata agtaaaaaca
     1141 aaacacctga acaaagacta gccaaagtgt cgatcccaaa taataactta atgcaatgat
     1201 aacaaagtaa aagagccctc ctgttgtaaa agcatcacta tagggaagtt gccccttcgt
     1261 taacatcatt cctatatata agttttggga ctgaagacca ttggctgcat ctgttaaaaa
     1321 aggtactgca attgttaaag aactgataat aatgctaaag aaaagcagat agcatttatg
     1381 aagccgaggt actttagttt catattgagt ggtgtgtact cgtgggccat cgttggccag
     1441 ttctatcgtc tcttttcgga tgaattcatc gtttaactcg atcactattt ttctccttga
     1501 aatatcatat tctctaacga ttcaataaaa gcaatatttc
//
LOCUS       DAUELZ010000171         1521 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.172, whole genome shotgun sequence.
ACCESSION   DAUELZ010000171 DAUELZ010000000
VERSION     DAUELZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.172"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..>1521)
                     /locus_tag="VHI09_001659"
     CDS             complement(<1..>1521)
                     /locus_tag="VHI09_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEQ6121502.1"
                     /translation="TGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYR
                     IIADQEISFYLFRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKL
                     YLPEFIVGELSKITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQK
                     TKKKHVLLWMKLFAINGLTLVVLSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELI
                     FFTTIFFKLCSAMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFD
                     TSHSKTVIAHRGLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKR
                     LAGVNKTIRNLTLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEP
                     GNYVDLFLETYHRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFV
                     DFYVIEDFSYRSYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTN"
BASE COUNT          558 a          265 c          236 g          462 t
ORIGIN      
        1 gattagttaa agctggttga tctgtaataa ttccctgaat aggctttagg agataatgct
       61 ctatgcgctt gggatcatta atagtccaaa cgtaaatttc tttattattc caaaaagctt
      121 gggacgacaa ataagaccga taagaaaagt cttcaatgac atagaaatca acaaattcat
      181 ctccaaaaaa gccaaattga attggtatga tataaccagt cgtaattgat ggattttttt
      241 tcttgatagc ttctattact tctaaatcta aagacatgac tttattatct ttgcttatac
      301 caagtcgatg ataagtttct aaaaacaaat cgacataatt tccaggttct gtaccaattg
      361 gcttgagttc aataagtaat ggcatattca acttcttagc cttttgataa aaagtgtcaa
      421 aagaaacaaa acgccctgaa aaatgtcctt gatgactcgt tagatgttca acttctttta
      481 aggttaagtt gcgaatcgtc ttatttactc cagctaaacg cttcaatcga ttatcatgag
      541 acaccacaaa gtgattatcc ttagtcaaga ttagatccag ttctacataa tcacttcctg
      601 ctttcttagc accttcaagg gcttccagag aattttctac acctgctgat acaagtcctc
      661 tatgcgcgat aactgtctta gagtgagatg tgtcaaaaaa agttaaacgt tcaagagatt
      721 gataagcaaa acctactgtc accacgataa agattacaac atatgcctta ttacttcttc
      781 ttggctcatc atattgcttt tgtggttcaa ttgcctcttt taaaatcatt gctgaacaga
      841 gtttaaaaaa aatagtagta aaaaaaatga gctcccatgt aaaggtcaaa gctcccagct
      901 gaacaataat attattcccc ttaggattaa acatatcaac aaaaataaga atcatggaaa
      961 tagctagcga taagactaca agcgtaagac cattgattgc aaaaagtttc atccataaca
     1021 atacatgttt ctttttagtc ttttgccaac tctctctcca tgcttgagca accgtacgat
     1081 ggttgattgc tatcaatggt aaaaaatata ctaatcttag gttaaggtaa aacacaagaa
     1141 taagactgcc ataaagcaag tactttgtgc tagttatctt tgataattcc ccaacaataa
     1201 attctggaag ataaagcttt tgagtaatca cagaagataa accaatatgt aagactggaa
     1261 tagtcagtag gatataaagc aaaaaaagta ataattggta accagaaaat gttttccaaa
     1321 gcccccttag gtaataagaa aactgttttc taaaaagata gaaactaatt tcttgatccg
     1381 caataattcg atatagagca aaaaactcaa ggtgaataaa agcagctaat aaaaataaat
     1441 aggcacctaa taaagcaagt gctataggat tttttaaaaa agtcacaaca ttgtctttat
     1501 ctaaatgata ttgaccagta a
//
LOCUS       DAUELZ010000172         1496 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.173, whole genome shotgun sequence.
ACCESSION   DAUELZ010000172 DAUELZ010000000
VERSION     DAUELZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.173"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            158..>1496
                     /locus_tag="VHI09_001660"
     CDS             158..>1496
                     /locus_tag="VHI09_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEQ6121503.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQITSGSQLEQSKESLSLNKTVLNKTVPSTSNWEICDFITKGNTLVGLSKSGVE
                     KLSQTDHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKE
                     IINEGEVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLAL
                     KQIDLPDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVI
                     GEASFQDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENT
                     YVNPDKSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQ"
BASE COUNT          540 a          237 c          280 g          439 t
ORIGIN      
        1 attaccttaa aacaggaagt actagagtat ttaaaataag tgatagactt ggatattatt
       61 aggtcgacta gtgttctttt caataattta atatcgttat tattagtatc agccaatcaa
      121 atgtggataa gttggctttt agaaaggttt taccacaatg aagaaacatc ttaaaacagt
      181 tgccttgacc ctcactacag tatcggtagt cacccacaat caggaagttt ttagtttagt
      241 caaagagccg attcttaaac aaactcaagc ttcttcatcg atttctggcg ctgactacgc
      301 agaaagtagc ggtaaaagca agttaaagat taatgaaact tctggccctg ttgatgatac
      361 agtcactgac ttattttcgg ataaacgtac tactcctgaa aaaataaaag ataatcttgc
      421 taaaggtccg agagaacaag agttaaaggc agtaacagag aatacagaat cagaaaagca
      481 gatcacttct ggatctcaac tagaacaatc aaaagagtct ctttctttaa ataaaacagt
      541 tttaaataaa acagtgccat caacgtctaa ttgggagatt tgtgatttta ttactaaggg
      601 gaataccctt gttggtcttt caaaatcagg tgttgaaaag ttatctcaaa ctgatcatct
      661 cgtattgcct agtcaagcag cagatggaac tcaattgata caagtagcta gctttgcttt
      721 tactccagat aaaaagacgg caattgcaga atataccagt agggctggag aaaatgggga
      781 aataagccaa ctagatgtgg atggaaaaga aattattaac gaaggtgagg tttttaattc
      841 ttatctacta aagaaggtaa caatcccaac tggttataaa catattggtc aagatgcttt
      901 tgtggacaat aagaatattg ctgaggttaa tcttcctgaa agcctcgaga ctatttctga
      961 ctatgctttt gctcacctag ctttgaaaca gatcgatttg ccagataatt taaaagcgat
     1021 tggagaatta gctttttttg ataatcaaat tacaggtaaa ctttctttgc cacgtcagtt
     1081 aatgcgatta gcagaacgtg cttttaaatc aaaccatatc aaaacaattg agtttagagg
     1141 aaatagtcta aaagtgatag gggaagctag ttttcaagat aatgatctga gtcaactaat
     1201 gctacctgac ggtcttgaaa aaatagaatc agaagctttt acaggaaatc caggagatga
     1261 tcactacaat aaccgtgttg ttttgtggac aaaatctgga aaaaatcctt atggtcttgc
     1321 tactgaaaat acctatgtta atcctgataa gtcactatgg caggaaagtc ctgagattga
     1381 ttatactaaa tggttagagg aagattttac ctatcaaaaa aatagtgtta caggtttttc
     1441 aaataaaggc ttacaaaaag taaaacgtaa taaaaactta gaaattccaa aacagc
//
LOCUS       DAUELZ010000173         1381 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.174, whole genome shotgun sequence.
ACCESSION   DAUELZ010000173 DAUELZ010000000
VERSION     DAUELZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.174"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(75..1097)
                     /gene="trpS"
                     /locus_tag="VHI09_001661"
     CDS             complement(75..1097)
                     /gene="trpS"
                     /locus_tag="VHI09_001661"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEQ6121504.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPRQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
BASE COUNT          416 a          294 c          205 g          466 t
ORIGIN      
        1 aaatgacaaa ttaggtcctg ctttgataat agtaattatc ttatcattac taacttagct
       61 ttttctctaa aacattaaaa ataattaata cccattgctg acttcacttc tgataaagtc
      121 ttggctgcca cagttcttgc tttttgacta ccttcttgta acatacggaa cacctctccc
      181 atatctttag cgtactccaa acgtctttca cgaataggtg ctaattcacg ttctaaaata
      241 tctagaaggt agcgtttcgt tttcacatct cctaaaccac ctctttgata atgctctttc
      301 attgcttcga tatcagcttg atcttctttt ctagcaaaaa tatccaaata atgaaagacc
      361 atattccctt caatttgacc aggatcttct attttaatat gatttggatc agtatacatg
      421 ctcatcactt ttttgcgaac ggtatctgca tcatctgaaa gatagattcc atttcccaat
      481 gatttagaca ttttggcatt gccatcaaga ccaggtaagc gtccagcttt ttcattttct
      541 ggataaatac cttcaggttc tactaaacag tctgtatggt aagtatgatt aaaacttctc
      601 acaatttcac gtgtttgttc aatcatcggt ttctggtcgt tacctacagg tactaaattc
      661 gctttaaatg ctgtaatatc agcggcctgt gatactggat aaaccaaaaa accagatgga
      721 atactttcgc caaaaccttt ctgagcaatt tcggttttaa cagtgggatt tctttccaaa
      781 cgtgctaatg ataccagatt catataatac atgctcaatt cagctagctc tggaatctga
      841 ctttgaataa aaatcgttga ttgcctcgga tccaaaccga cagagaggta atccaaggca
      901 acatttccaa tagattcttg aattaattcg gattctttag catgatccgt cagtgcctgt
      961 tgatcggcca agaaaacaaa catcttatac ttgttttcat tttgtaaaaa gacacgattt
     1021 ttaagacttc cgacataatg acctaaatgt aattttccag ttggtctatc tccagttaaa
     1081 ataataggtt ttgtcatcat tttctcccta actatctctt gatatagcaa aaagtcccca
     1141 cgctattaaa acgtgagggc gttattacaa gtaatacgcg gtaccacctc aattagcatc
     1201 tgctttatca aaacgagata ctatctcaat cttctcaatg aaagaagctg cacggtaagg
     1261 ggtgccaacc tgaaagttat ggtttccttt caaataatca ttagtccatt tcaccttttc
     1321 tgttgttagc ttgcaccgac cactaactct ctaaaaacat gaaaagaata ctcttctaat
     1381 t
//
LOCUS       DAUELZ010000174         1381 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.175, whole genome shotgun sequence.
ACCESSION   DAUELZ010000174 DAUELZ010000000
VERSION     DAUELZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.175"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            218..1339
                     /gene="mnmA"
                     /locus_tag="VHI09_001662"
     CDS             218..1339
                     /gene="mnmA"
                     /locus_tag="VHI09_001662"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEQ6121505.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSQVTVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT          426 a          226 c          307 g          422 t
ORIGIN      
        1 tttataataa aaaattattg agtaataaaa tgattggcat tctacgcagt aattgttata
       61 ataaggtttg cgctgatggc gcttcaaaat cgaccttcaa atcgactttt gaaaacgacg
      121 accttcaatc gtacgttaac tgataagaaa aagaatcata atattcataa tatgatgatt
      181 atctttttct atcttattat tgatgggaga attttttatg acagataact caaagatacg
      241 tgttgtcgtt ggtatgagtg gtggcgttga ttcatctgta acagctctac ttttaaagga
      301 gcaaggttat gatgttattg gtgtcttcat gaaaaactgg gacgatacag atgaatttgg
      361 tgtttgcaca gctactgaag attacaaaga tgtagcagcc gttgctgatc aaatcggtat
      421 tccttactat tcagtcaatt ttgaaaaaga atattgggac agagtatttg aatatttctt
      481 ggccgaatac cgagcaggtc gtactccaaa tcccgatgtc atgtgtaata aggaaataaa
      541 atttaaagct ttccttgact atgccatgac tttaggtgca gattatgtag caacgggaca
      601 ctatgctcaa gtcaagcgtg atgaaaatgg tacggttcac atgttacgtg gagctgataa
      661 tggcaaagac caaacctact ttttaagtca actatcacag gaacaactac aaaagacttt
      721 attccccctt ggtcatttgc aaaagtcaga agttagagaa attgctgagc gagcagggct
      781 tgcaacggct aaaaagaaag attcaacagg tatttgtttt attggtgaaa agaatttcaa
      841 acagtttttg agccagtact taccggcaca aaaaggtcgc atgatgacta ttgatggtcg
      901 cgatatggga gaacatgccg gtcttatgta ttatactatt ggtcagcgtg gtggtctggg
      961 cattggtggt cagcatggtg gggataatca accttggttt gttgtcggta aagatttgtc
     1021 gcaaaatatt ttatacgttg gtcaaggttt ctaccatgaa gccctaatgt caaatagtct
     1081 tgacgcctca gtcatccatt ttacacgtga gatgccagaa gagttcacat ttgaatgtac
     1141 tgctaaattt cgctatcgtc aacccgattc acaggtgacg gtgcatgttc gaggagataa
     1201 ggcagaagtt gtttttgcag agccacagcg cgcgattact cctggtcaag ctgttgtttt
     1261 ctatgacgga aaagaatgcc ttggtggtgg tatgattgac atggcttata aaaatgggca
     1321 gccttgtcag tacatctaat gaatctttta ttaagataaa aagaaaggga cattcaacaa
     1381 t
//
LOCUS       DAUELZ010000175         1315 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.176, whole genome shotgun sequence.
ACCESSION   DAUELZ010000175 DAUELZ010000000
VERSION     DAUELZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.176"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(38..1264)
                     /locus_tag="VHI09_001663"
     CDS             complement(38..1264)
                     /locus_tag="VHI09_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEQ6121506.1"
                     /translation="MKRHFLLLTFYLFLTGLTAGLVAFILTKAIHLIQSLSFGFSQGS
                     FSTMIASVPPQRRALSLLFAGLLAGLGWHLLAKKGKDIQSIQQIIQDDISFSPWTQFW
                     HGWLQLTTVSMGAPVGREGASREVAIALTSLWSQRCNLSKADQKLLLACASGAALGAV
                     YNAPLATILFILEAILNRWSLKNIYAACLTSYVAVETVALLQGRHEIQYLMPQQHWTL
                     GTLIWSVLAGLILSLFAHAYKHLLEHLPKADAKSQWFIPKVLIAFSLIAGLSSFFPEI
                     LGNGKAGLLFFLHEEPHLSYISWLLVAKAVAISLVFASGAKGGKIAPSMMLGGASGLL
                     LAILSQYLIPLSLSNTLAIMVGATIFLGVINKIPLAAPVFLVEITGQSLLMIIPLALA
                     NLIFYFSYQFYRFILK"
BASE COUNT          454 a          247 c          294 g          320 t
ORIGIN      
        1 tcatccgaac ctttttatta aaaaagtaat taactaatca tttaaggatg aaacgataaa
       61 actgatagct aaaataaaaa atgaggttag ctaatgctaa agggataatc attagtaacg
      121 attggcctgt aatttcaact agaaaaactg gtgccgccaa gggtatctta ttgatcacac
      181 ctaaaaagat tgttgcacca accataatgg ctaacgtatt tgatagagac aaaggaataa
      241 ggtactgact aaggatagct agtagtaagc cactagcacc tccaagcatc atagaaggag
      301 caattttacc cccttttgct ccagaagcaa aaactaaaga gatagcgaca gctttggcaa
      361 caagtagcca gctaatatag ctcagatgtg gttcttcatg gagaaaaaag agtaatccag
      421 ctttgccatt tcctaaaatc tctgggaaga aactgctgag tcctgcgata aggctaaaag
      481 cgatgagaac tttcggaata aaccactgac ttttggcatc agccttagga aggtgttcca
      541 gaagatgttt gtaagcgtga gcaaagaggg aaaggataag accagccagg acggaccaaa
      601 taagggttcc tagcgtccaa tgttgctgag gcattaggta ttgaatctca tgtcggcctt
      661 gtaataaagc aactgtttcc acagccacat agctcgttag gcaggcagcg tatatatttt
      721 taagggacca acggttaaga atagcttcca agataaataa aatagtggct aagggagcat
      781 tgtagacagc accaagggca gcgccagaag cgcaagctaa taaaagtttt tgatcggctt
      841 ttgacaagtt acatctttgg ctccaaagag acgtcaaggc aatagcgact tcccgcgaag
      901 ctccttctcg gccgacagga gctcccatag atacggtcgt taattggagc caaccatgcc
      961 aaaactgtgt ccagggacta aatgagatgt catcttgaat gatttgctga atagattgaa
     1021 tgtcctttcc ttttttagcc aataggtgcc acccaagacc tgcaagaagt ccagcaaaga
     1081 ggagtgaaag agctcgtctt tgtggtggaa cactagcaat catggtgcta aaagaacctt
     1141 gactgaatcc aaaactgagg gactgaatca ggtgaatggc ttttgttaag ataaaagcaa
     1201 caaggccagc tgttaaacct gttaaaaaga ggtaaaaggt caagagtaaa aagtgtcgtt
     1261 tcataagact attataacaa aagttaagaa aagagactga tagattatca gtctc
//
LOCUS       DAUELZ010000176         1314 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.177, whole genome shotgun sequence.
ACCESSION   DAUELZ010000176 DAUELZ010000000
VERSION     DAUELZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.177"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            22..>1314
                     /locus_tag="VHI09_001664"
     CDS             22..>1314
                     /locus_tag="VHI09_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEQ6121507.1"
                     /translation="MISYRKTFVAQIDARDCGVAALASIAKYYGSDYSLAHLRELAKT
                     NKEGTTALGIVKAAKLMGFETRAIQADMTLFDIEDVPYPFIVHVNKEGKFQHYYVVYQ
                     NKKNYLIIGDPDPTVKVTKMTKERFTSEWTGVAIFLAPEPSYKPHKDKKNGLINFLPL
                     IFKQRSLIFYIILASLLVTLINIVGSYYLQGILDDYIPNQLKSTLGIISIGLIITYIL
                     QQMMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANAII
                     DALASTILSLFLDVSILTIVGTVLLVQNTNLFLLSLVSVPLYIVIIFIFMHPFEKMNN
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQKIDSEFVDYLDKSFKLSKYSILQS
                     SLKQGAQLILNVIVLWFGAKLVMGGKISVGQLITFNTLL"
BASE COUNT          403 a          246 c          225 g          440 t
ORIGIN      
        1 agatagaaag gtcttttaca aatgatatcc tatcgaaaaa catttgttgc tcagattgat
       61 gctagagact gtggtgtggc tgctcttgct tccatcgcta aatattacgg ttcagactac
      121 tctttagctc atttaagaga gttagctaaa acaaataaag agggaacaac agcgcttggc
      181 atcgtcaaag ctgccaaatt aatggggttt gaaacaagag ctattcaagc agacatgacc
      241 ctctttgata tagaagatgt cccttatcct tttattgttc atgttaataa agaaggtaaa
      301 ttccaacact actatgtcgt ctatcaaaac aaaaaaaatt atctgataat cggcgaccct
      361 gacccgacag taaaagtcac taagatgaca aaggagcgat ttacttctga atggacgggt
      421 gtcgctattt ttctagcacc cgagcccagc tacaagcctc ataaggacaa aaagaacggt
      481 ctaataaact ttttacctct tatttttaaa cagcgatctc tgattttcta tatcatatta
      541 gctagcctac tcgttactct gattaatatt gtaggttctt attacctaca aggtatatta
      601 gatgactata ttccaaatca actgaaatca acactcggca ttatttcaat tggtcttatc
      661 attacctata tcctccaaca gatgatgagt ttttcgcgag attatctcct gactgtactg
      721 agtcagcgat taagcattga cgtcatttta tcttacattc gccatatttt tgaattaccc
      781 atgtcttttt ttgcaactcg cagaacagga gaaatcattt cacggtttac agatgctaat
      841 gctattattg atgcactcgc ttctactatt ttgtctcttt ttcttgatgt cagtatttta
      901 actatcgtag ggacagtcct gttagtgcaa aataccaatc tttttcttct atctctggtt
      961 tcagtccctc tatatattgt tattatcttt atatttatgc acccatttga aaagatgaat
     1021 aatgatgtca tgcagagtaa ctctatggtt agctcagcca ttatcgaaga tatcaatggc
     1081 attgaaacta ttaaatcact gaccagtgaa gaaaatcgtt atcagaaaat tgatagtgaa
     1141 tttgtggact atctggataa atctttcaag ttaagtaagt actctatttt acaaagcagt
     1201 ctcaaacaag gggcgcaact aatccttaac gtgatagtct tatggtttgg ggccaaatta
     1261 gttatgggag gaaaaatttc tgttggtcag ttgattacgt ttaatactct ctta
//
LOCUS       DAUELZ010000177         1282 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.178, whole genome shotgun sequence.
ACCESSION   DAUELZ010000177 DAUELZ010000000
VERSION     DAUELZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.178"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..704
                     /locus_tag="VHI09_001665"
     CDS             <1..704
                     /locus_tag="VHI09_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEQ6121508.1"
                     /translation="PYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIASKVVSVAEGQV
                     MSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNYIGCRLENGLKL
                     TSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSDGREDKRGATQV
                     AIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGTGIPAVLIKGLD
                     YKWFENTSIKEALNE"
     gene            697..>1282
                     /locus_tag="VHI09_001666"
     CDS             697..>1282
                     /locus_tag="VHI09_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEQ6121509.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLMLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVGSHPHLAKKMFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVISYLGISLEEFKK"
BASE COUNT          469 a          160 c          262 g          391 t
ORIGIN      
        1 ttccttattt gaaagaaggg gatagtatag aaaagcttat tgaagaaagc attaaaactt
       61 cagaattttt tatcgaagac aatgatgtgc tttgcatagc ttctaaagtt gtatctgtcg
      121 cagaaggcca ggtgatgtct ttaaacgaga tacaagtgag tgatgtagca aaagaaatcc
      181 acaggaatat tcctcgtaaa gatcctagaa taattgaaat tatgctaaat ttagtcaata
      241 gagatctgtc acgtttagat atcaaaaaaa attatattgg ttgtcgcttg gaaaatggtt
      301 taaagttgac aagtggtgga atcgatagaa aatcagtaga tgaagtattt cttttaccta
      361 ataatccgga tgcttctgca aaaagaattt cggaatatct taaaaaatca cttggaaaaa
      421 atgttgcagt tgttataaca gatagtgatg gtagagagga taaaagaggt gctactcaag
      481 tagcaattgg tatttatggt attcatcctt tgagaaaaac agaagtaata gattcacaag
      541 gagagacaat aaaattccaa gaagagactt tgtgtgatat gattgcagct tgtgctggct
      601 tagtaatggg gcaacgtgga actggaattc cagctgtcct cattaagggc ttggattata
      661 aatggtttga aaatactagt attaaggaag cgttaaatga gtagaaatca ggactgtaag
      721 aatattctaa tcattggttt tggaaaaatt gggaaaatta aagcgtacaa gtggctatca
      781 cgcggatatg aggtatatgt caaagattta aaattctcca gcatgaaata ccttaataca
      841 aatagtgatg tgttggatga tttatcacga caatatttta ctattgagat ttgtactccg
      901 acaaatcacc accttatgct attgaaaacc gttataacaa ggtatgtcta ttcttatatt
      961 agcattgaaa aaccactgtg taacaggtta gaagatttag aagaaatgaa gtcgctagta
     1021 ggaagtcacc cacatctagc aaaaaaaatg tttgccagtg aacaatactt cttttcgaaa
     1081 attttggaaa aactgttaca agtagattgt ttctctctag ataaaatcaa ggaaataaca
     1141 gttaattttt ctaaagatag gattgaggat aacgagaatg ggagattttt agataaagag
     1201 atattgggct atggaataga gattcctcat atcattgctg tcatttctta tttaggaatt
     1261 tcattagaag aattcaaaaa ag
//
LOCUS       DAUELZ010000178         1250 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.179, whole genome shotgun sequence.
ACCESSION   DAUELZ010000178 DAUELZ010000000
VERSION     DAUELZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.179"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(135..452)
                     /locus_tag="VHI09_001667"
     CDS             complement(135..452)
                     /locus_tag="VHI09_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="role in replication"
                     /protein_id="HEQ6121510.1"
                     /translation="MLTLYKRQIAEVWEIGRETPQPEWVRDAFRKNYICWLDEHVRIL
                     MTGLNPSLTTNLKISATGTVGGGFAGYGMYVLGYPGDFLDVTNHRVISAKKFHKEYQI
                     IDG"
     gene            complement(501..728)
                     /locus_tag="VHI09_001668"
     CDS             complement(501..728)
                     /locus_tag="VHI09_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEQ6121511.1"
                     /translation="MNTKTMEQFEVMNSEMLAGVEGGWGGWGEAIAGLLGGFAPSPTL
                     DQLNGKWPIRKPSKPCSPYGTGGTPNTCNGL"
BASE COUNT          393 a          289 c          171 g          397 t
ORIGIN      
        1 tatctttgta ccaaatagat gatgaataaa tgaaaaccga caaagaaaat acttcctaca
       61 atcttccaaa gagctccttt ttgttgtaaa ccctcaaaat taaaccagtc ataaaagttc
      121 atagtctact ttttctatcc atcaataatc tgatattctt tatggaattt tttggcagaa
      181 ataactcgat ggttggtcac atccaagaaa tcacctggat aaccaaggac atacatacca
      241 tatccagcaa atcctccacc tactgttcct gttgcactaa tctttaaatt tgttgtcagc
      301 gaaggattta gtccagtcat gagaatacga acatgctcat ccaaccaaca gatgtaattc
      361 ttgcgaaaag catcacgtac ccactctggc tgaggcgttt ctctcccaat ctcccaaact
      421 tctgcgattt gtcttttgta tagagtcagc atatctacct ccacttcctt tattgcataa
      481 tcacttaaat atgtcctaat ttataaacca ttacacgtat taggtgtccc accagttccg
      541 taaggactac aaggtttgga aggttttcta attggccatt tgccatttaa ttgatctaat
      601 gttggtgaag gagcaaagcc tcctaataga ccggcaattg cttctcccca accgccccag
      661 cctccttcaa caccagcaag catttcagag ttcatcactt caaattgttc cattgttttc
      721 gtattcatca tgaatatctc cttatataat ttttatattt tgtccagttg caaccttgac
      781 atattcagta tagcgcttct taaaaaaatc ctgcactatt tcctaaacag tcatttaact
      841 ttcctaaatg ggaagtcttt ttaacattca taatttcaaa aacacccata ttatcttaat
      901 tttatatcca tgagtccaag actcactcta ccattcaaga tgtttcgatg tccgtttaag
      961 atatagtcat aaacaaaatg caaagccagc gaaatgcctc taagttctct ctcctattcc
     1021 ctagaaagca aggtcagtat acatagcttg gctttgacat aaaatgctta ttatactaac
     1081 gaagctacaa atccgacaac atagtcaacc gcatctttaa taacattaac ggaaagtgta
     1141 atctgaaact acaatttttt gcccaagaag cttcttcttc cagatgcgtc ctgtaatcaa
     1201 accaacacaa taactttttt gggagccacc tgaaatactt gttagctcag
//
LOCUS       DAUELZ010000179         1244 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.180, whole genome shotgun sequence.
ACCESSION   DAUELZ010000179 DAUELZ010000000
VERSION     DAUELZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.180"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(573..>1244)
                     /locus_tag="VHI09_001669"
     CDS             complement(573..>1244)
                     /locus_tag="VHI09_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ6121512.1"
                     /translation="KAYRPGEGVKARKEAAKANLEKVAKETKALISGDRYLSETEKAV
                     QKQAVEQALAKALGQVEAAKTVEAVKLAENLGTVAIRSAYVAGLAKDTDQATAALNEA
                     KQAAIEALKQAAAETLAKITTDAKLTEAQKAEQSENVSLALKTAIATVRSAQSIASVK
                     EAIDKGITAIRAAYVPNKAVAKSSSANHLPKSGDANSIVLVGLGVMSLLLGMVLYSKK
                     KESKD"
BASE COUNT          376 a          254 c          214 g          400 t
ORIGIN      
        1 gtaaacaatc aagtagaaat gtctttatta agcgaatcaa tcaaggttgt agagtaaata
       61 ctggactaga tttcatgtag aaattagtaa aagttaggag attttttggt ggtttcaaac
      121 gataacatca tcttcttata acaagaactt cactcagaga taaactattc taatacttta
      181 gtagcctctt ttgagcttgt agcacgaaaa ttcgctcgaa ctgatttaaa ataggcattt
      241 tatctgctcg cttgatccct actgcgcgac caagaaacct tttattcact agaacaatta
      301 acccttaaaa ccaaggagga agtccactgg tagaactacc atcaccttca cagtaatctt
      361 aattatcaaa cacccataac taaatgagtc atcgtttgaa caaaaaacgc cttataaatt
      421 ctgtacagaa aactgttgcc ttttcaattg aactttgaaa tgttttcaat acaatccgtt
      481 cttgggccct tacgatccaa aaaaccaagg tgatttcaaa atcaccttgg tttgaaaata
      541 tagtagaaag caatttggat aaagccaggt tcttagtctt tactttcttt tttcttgcta
      601 taaagcacca tacctaaaag aagagacata actcctaagc caacaagaac aattgagttt
      661 gcatcacctg attttggaag atggttcgct gacgatgatt ttgcgactgc cttattaggc
      721 acataggctg cacggatagc agtaatacct ttatctattg cttctttcac agacgcaata
      781 gattgtgctg aacgaacagt cgcaatagcc gtcttaagcg ctaatgatac attttctgat
      841 tgttcagctt tttgagcttc agtcaattta gcatcagttg taatcttagc aagtgtttct
      901 gccgcagctt gtttaagagc ttcaatagca gcttgtttcg cttcgttaag agcagctgtt
      961 gcttgatcag tatctttagc taaaccagca acatatgctg aacggatagc tacagtacca
     1021 agattttctg ccaacttaac agcttcaact gtcttagcag cctcaacttg accaagtgct
     1081 ttcgcaagag cttgctcaac agcttgtttt tggactgctt tttcagtttc gctcaagtaa
     1141 cggtctcctg aaataagagc tttagtttct ttagctactt tttcaagatt agctttagca
     1201 gcttctttac gtgctttaac accttcacct ggacggtaag cttt
//
LOCUS       DAUELZ010000180         1241 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.181, whole genome shotgun sequence.
ACCESSION   DAUELZ010000180 DAUELZ010000000
VERSION     DAUELZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.181"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..>1241
                     /locus_tag="VHI09_001670"
     CDS             <1..>1241
                     /locus_tag="VHI09_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ6121513.1"
                     /translation="LLTLENLVILVLSYLVSLLLGPTLYFLAVLAITHLLELTLEVQW
                     FITANEIIESLGILVVVFLINVITNGLIISKQSLIEFVNFSRKAEKKIKIRKVRAITA
                     ITALLLSYILCLATVFSPTRNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFVVSLL
                     KGNKKRLYRPLSNIIYPKFNYRIATKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGI
                     ERLTPSAIEYNVESENGQVNVTTILENDQVSLVDVDLLRLNTNPEVTITESGQTIPYF
                     DIINYSDYKELMKAQGRINSIEGSKSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVS
                     THNVFSFSTSVTTLVVSDKLYAKLSSCFPEKEMTIRTFNGTSIRSSEAFYNQFSMVPD
                     VISSYSKEYTVKTANIATYIF"
BASE COUNT          407 a          173 c          222 g          439 t
ORIGIN      
        1 aattactaac tttagaaaat cttgttattc tagttctgag ttacttggta agtttattgc
       61 tgggaccgac tttatatttt ttagctgtac tggcaattac tcatctattg gaattaacat
      121 tggaagttca gtggtttatt acagctaatg agattataga gtctttagga atattagttg
      181 tagtttttct gattaatgtc atcacaaatg gacttatcat tagtaaacag tctttgattg
      241 aatttgttaa tttctcaaga aaggctgaga aaaaaattaa gataagaaaa gtcagagcta
      301 ttactgctat tactgcattg ctattatcat atattttatg tttggcgaca gtattttcac
      361 ccacacgaaa tatgctatta agcataggga tggtaccggt ttctctattg ataattgttt
      421 tagttgtttt gggaacagtg tttaccatca gatatggatt ggcttttgta gtttcgttat
      481 taaaaggaaa taaaaaaagg ttataccgtc ctctgtctaa tatcatctat cccaaattta
      541 actatcgtat tgcaacaaaa aataaattat taacagtctt gggaggtctt ttaacagtaa
      601 ccgtttcagt tgccggaatg atggtaatgc tctatgctta ttctcttaat gggatagaga
      661 ggttgactcc atctgccata gaatataatg ttgaatcaga aaacggtcaa gtcaatgtta
      721 caactatttt agagaacgac caagtgagct tggttgatgt tgacctattg agattgaaca
      781 ctaacccaga agtaactatc acagaatctg ggcaaacaat tccttatttt gatataatta
      841 attacagtga ttataaagag ttaatgaaag ctcaagggag aataaattct attgaaggta
      901 gtaagtcact cccattgtta ataaattatt atccaacaga aattagcctt ggaaaaacct
      961 ttaacttagg aaatgcatat gatgttactg taaaacaagt atcaacgcat aatgttttta
     1021 gtttttctac aagtgtcacg accttggttg tttctgataa attatatgct aaacttagtt
     1081 cttgttttcc agagaaagaa atgacaatta ggacttttaa tggaacttcg attaggtcaa
     1141 gtgaagcatt ttacaatcag tttagtatgg ttcctgatgt tatcagtagt tatagtaagg
     1201 aatacacagt aaagactgct aatattgcga cttatatctt t
//
LOCUS       DAUELZ010000181         1222 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.182, whole genome shotgun sequence.
ACCESSION   DAUELZ010000181 DAUELZ010000000
VERSION     DAUELZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.182"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            196..>1222
                     /locus_tag="VHI09_001671"
     CDS             196..>1222
                     /locus_tag="VHI09_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEQ6121514.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTDAIVQLVKTTICGTDLHI
                     LGGDVPACKEGTILGHEGIGIVKEVGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSS
                     HCEDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVL
                     PSHVKPGDNVCIVGAGPVGLAALLTVQFFSPANIIMVDLSQNRLEAAKTFGATHTICS
                     GSSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFN
                     LDELWIKNITLNTGLVNANTTEMLLNVLKTGKIDATRLITHHFKLSEVEKAYETFKHA
                     GVNNALKV"
BASE COUNT          394 a          214 c          236 g          378 t
ORIGIN      
        1 tttaagtaaa gcggtttcaa attcttataa tagtgccctt taagttctga ataaggatga
       61 agttattcaa caaagatgat ctaaaaaatt atcataatcg atttaaaaaa tattgaaaac
      121 cctttcttaa actattataa tatctgtgta aaaatgaatt ggttgattac ctctcaacct
      181 tataaggagt taaatatgaa agcagcaact tacctttcaa ccggaaatct acagctcata
      241 gacaaaccaa aaccagtcat tataaagcct acagatgcta tcgtccagtt ggtaaaaacc
      301 actatttgtg gaactgattt gcatatctta ggtggggatg ttcccgcatg taaagaagga
      361 acaatccttg gtcatgaagg aattggaatt gttaaggagg tcggtgatgc ggttactaac
      421 tttaaaatcg gagataaagt gattatttct tgcgtcacat catgtcatac ctgttattat
      481 tgtaaacgag gcctttcgtc acattgtgaa gatggcggtt ggattcttgg ccatttaatt
      541 aatggaacac aagcagaata tgtccatatc ccacatgcag atggtagtct ctatcatgct
      601 cctgatacga ttgatgatga ggcacttgtc atgctttctg atattttacc aacttcatat
      661 gaaattggtg tcttgccatc acatgtcaag cctggagata atgtttgtat tgttggggcc
      721 ggtccagtag gactcgcagc cctactaacc gttcaatttt tctctccagc caatattatt
      781 atggtcgact tatctcaaaa tcgccttgaa gcagctaaaa catttggagc cacacacacg
      841 atttgttcag gaagtagtga agaagtcaag gctatcattg acgatataac caatggacgt
      901 ggtgtcgata tttccatgga gtgtgttggt tatcccgcga catttgatat ttgtcaaaaa
      961 attatatcag taggtgggca tattgctaac gttggggttc atgggaaacc agttgatttc
     1021 aacctggatg aattatggat taaaaatatc accttaaaca ctggacttgt taatgccaat
     1081 acaactgaaa tgctactaaa tgtcttgaaa actggaaaga ttgacgcaac aagacttatt
     1141 acacatcatt ttaaactttc agaagttgaa aaagcttatg aaacctttaa acatgctggt
     1201 gtaaataacg ccttgaaagt ta
//
LOCUS       DAUELZ010000182         1214 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.183, whole genome shotgun sequence.
ACCESSION   DAUELZ010000182 DAUELZ010000000
VERSION     DAUELZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.183"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..359
                     /locus_tag="VHI09_001672"
     CDS             <1..359
                     /locus_tag="VHI09_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HEQ6121515.1"
                     /translation="KETRRSFLFENYTGKYKKDFKNLPIDLPIEYMKILNGVTSALDR
                     YDFFQEMDRYPMSYMKEYNRFGGTNDIYNTKFNLVGSFLHLSMNRLNGIDREFEEKVY
                     CFAYYTLNAQQYIEWE"
     gene            365..1054
                     /locus_tag="VHI09_001673"
     CDS             365..1054
                     /locus_tag="VHI09_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ6121516.1"
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIM
                     KILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVS
                     ESTILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIG
                     IQELREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIILEKEYDGSENLEEL
                     FNNQILFEKRR"
BASE COUNT          443 a          148 c          225 g          398 t
ORIGIN      
        1 ataaagaaac aaggcgctct ttcttatttg agaattatac cggaaagtat aagaaagatt
       61 tcaaaaattt gccaattgat ttacctattg agtatatgaa gattttgaat ggagtcacct
      121 cagctcttga tagatatgat tttttccaag aaatggatag atatcccatg tcttatatga
      181 aagagtataa tcgatttggt ggtacaaatg atatctataa tactaaattc aatttagtag
      241 gaagtttttt acatctatca atgaatcgat taaacgggat tgatagagag tttgaagaga
      301 aagtgtattg ttttgcttac tatacactaa acgctcagca atatatagaa tgggagtaac
      361 taatatgttg aaaatacaaa atctaaaaaa atcttatggt aagagaacca tattgaataa
      421 tgtcaatatg aatattccta agggaaaagt gtatgcttta atcggtccaa atggtgctgg
      481 aaaatcaact attatgaaaa ttttgacagg cttagttagt aagacaagtg gttctattat
      541 ttttgaaggt agagaatggt cacgtcggga tctgcgaaaa atcgggagta ttattgaaga
      601 accaccactt tataaaaatt tgagtgctta cgataatatg aaggtagtta caacaatgct
      661 tggtgtttca gaaagcacta tacttccatt attaaataaa gttggtctag gaaatattga
      721 caagagacca gtaaaacaat tttctcttgg aatgaagcaa cggttaggta tagctatctc
      781 tttaataaat tcacctaaac tacttatatt agacgaacct actaatggct tggacccaat
      841 tggaattcaa gaattaaggg aaattataga gtcatttaaa tcagaaggaa tgacaattat
      901 gatttcaagc catatactgt cagaagttga acatctagct gattttattg gatttatcta
      961 tgaaggaaag attattctgg aaaaagaata tgacggctct gagaatcttg aagagttatt
     1021 caataatcaa attttatttg aaaagaggag gtaggggcta tggtacaaat ttttttatca
     1081 aatttattaa aaataaaaaa tacgagtatt agcaagttga ttcttatttt ccctattatt
     1141 tgtgtgatgt tatctttatt gttttcagca ttaggtggtg aaaatatcct acgtttaact
     1201 gcggaaacaa cagt
//
LOCUS       DAUELZ010000183         1194 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.184, whole genome shotgun sequence.
ACCESSION   DAUELZ010000183 DAUELZ010000000
VERSION     DAUELZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.184"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            60..461
                     /locus_tag="VHI09_001674"
                     /pseudo
     CDS             60..461
                     /locus_tag="VHI09_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
     gene            complement(519..965)
                     /locus_tag="VHI09_001675"
     CDS             complement(519..965)
                     /locus_tag="VHI09_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ6121517.1"
                     /translation="MSYKYGFGRDTLSDISLTIKEGEKVSIVGISGSGKTILAKMIVN
                     FFEPYKGQISINHQNLKMIDKKVLRQHINYLPQQAHIFSGSILENLTLGANHSTSQED
                     ILINIRNDIHILKFDTYFQTGQNIKKEKRIKRKIFHSYFIYIYFSM"
BASE COUNT          387 a          200 c          189 g          418 t
ORIGIN      
        1 tatcatatat cctaaaagag aacgatgtaa gacttgaata actatcataa ttttctccac
       61 gtattgaaca accaattgac tatgtccatc atcaacaagg tcaaacgtta gctgaagact
      121 cctttatttt taaagatgcc aagactcagg ttcagattcc attcagattc catttgcaga
      181 cttactttat attgaaacgt catatattgc tcataaattg gttctttatt ctactagaga
      241 aaggattgaa ttttatgggg agttatctga aatcgttaag caagaaccaa gattatttca
      301 atgccatcga tcatttgttg ttaatcccta caatatatct tcaatacaac gattagaacg
      361 taagatttat ttaaaaaaac ggttcatctt gtctagtatc aaggctaaaa gttactgctc
      421 taaaagaagc agttgaaaat ctacataata aggagactta gatgagtagc atcttctttc
      481 tagcacttac ttttactcat gtcattattt tgataatttt acatgctaaa gtatatgtat
      541 ataaagtaac tgtgaaaaat ctttcgcttt atacgctttt ctttttttat attctgccca
      601 gtttggaaat aggtatcgaa tttcaagata tgaatatcgt ttctgatatt tatcagaatg
      661 tcctcttgac tagtggagtg attagcacct agggttaagt tttctaagat agatccacta
      721 aagatatgag cttgttgggg gagatagttg atatgctgac gaagcacttt tttatcaatc
      781 attttaagat tttgatgatt aatagaaatc tgtcccttgt aaggttcaaa gaaattaaca
      841 atcattttag ctaatattgt cttgccagaa ccactaatac caactatact gactttttca
      901 ccctccttaa tggtcaaact gatatcagat aaggtatcac gaccaaagcc gtatttataa
      961 gacaagtctg caaattggat atctccatat agacaatagg tatctgttaa tgcctgttct
     1021 ttttggaact cagatttaac caggtaaact tcattgagac gattattagc taccttagcg
     1081 gattgcagtt tagtttgaag attaatgata ttttaccatt cataactagt cgtgcaccca
     1141 cccataagat agtaacgttt aaaatgagtt gtgctccttg ctttagacta cttt
//
LOCUS       DAUELZ010000184         1159 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.185, whole genome shotgun sequence.
ACCESSION   DAUELZ010000184 DAUELZ010000000
VERSION     DAUELZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.185"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..1152
                     /locus_tag="VHI09_001676"
     CDS             <1..1152
                     /locus_tag="VHI09_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ6121518.1"
                     /translation="GSLDNKLTVDDESRDAPAGIAHFLEHKLFEDESGGDISLKFTQL
                     GAETNAFTTFNQTSFFFSTASKFQENLELLQYFVLSANITDESVSREKKIIGQEIDMY
                     QDDADYRAYSGILQNLFPKTSLANDIAGSKESIQKITKILLETHHTYFYQPTNMSLFI
                     VGDIDIDETFLAIQRFQTTLSYPDRKRVTVDPLHYYPVIKSSSVDMDVTTAKLVVGFR
                     GYLTLTQHSLLTYRIALKLFLSMLIGWTSKIYHTLYEDGKIDDSFDVDVEIHHNFQFV
                     LISLDTPEPIAMSNYIRQKLATIKISKEFTNEHLNLLKKEMYGDFIQSLDSIEHLTHQ
                     FSLYLSDSDKETYFDIPKIIERLTLKDVVTIGKAFFEKADASDFTVFPK"
BASE COUNT          401 a          195 c          183 g          380 t
ORIGIN      
        1 ggttctcttg acaataagtt gacagtcgat gacgaatcac gagatgctcc agcaggtatt
       61 gcacactttt tagaacataa actctttgaa gatgaatcag gtggagatat ttcattaaag
      121 tttacacaac taggagcaga gacaaatgcc tttacaactt tcaaccaaac gagtttcttc
      181 ttttcaacag ctagtaaatt tcaggaaaat ttggaactcc ttcaatattt tgttctttct
      241 gctaatataa cagatgaatc tgttagtaga gaaaaaaaaa taatcgggca agaaattgat
      301 atgtatcaag atgatgctga ctaccgtgca tatagtggaa tccttcagaa cttatttcca
      361 aaaacaagtt tagctaatga tattgctggc tcaaaagaat ccattcagaa gatcacgaaa
      421 atattattgg aaacgcatca tacttatttt taccaaccta ctaatatgag tctattcatt
      481 gtcggtgata ttgatattga cgaaactttt ttagctattc aaaggtttca aaccacatta
      541 tcctatccag atagaaaaag ggtaactgtt gatcctttac actactatcc tgttattaaa
      601 tcatcttctg ttgacatgga tgttactact gctaagctgg ttgttggttt tcgagggtat
      661 ctgaccttaa ctcagcattc actattaacg tatcgtattg ccttgaagct atttttatca
      721 atgcttatag gttggacttc taaaatctat catacgctat atgaagatgg taaaatagac
      781 gattcatttg atgtggatgt tgaaattcat cataattttc aatttgtatt aatatcactt
      841 gatacaccag agcctatcgc aatgtctaac tatatccgtc aaaaattagc cactataaag
      901 atatcaaaag agtttacaaa tgagcactta aatctattaa aaaaagaaat gtatggtgac
      961 tttattcaga gtttagactc tatagaacat ttaacccatc agttcagtct atacctgtct
     1021 gattctgata aagaaacata ttttgatatt ccaaaaatta tagaaagact cactctaaaa
     1081 gatgttgtta caattggaaa agcatttttt gaaaaagctg atgcttcaga cttcacagtc
     1141 ttcccaaaat agatgaaaa
//
LOCUS       DAUELZ010000185         1153 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.186, whole genome shotgun sequence.
ACCESSION   DAUELZ010000185 DAUELZ010000000
VERSION     DAUELZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.186"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            163..1122
                     /gene="nrdF"
                     /locus_tag="VHI09_001677"
     CDS             163..1122
                     /gene="nrdF"
                     /locus_tag="VHI09_001677"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ6121519.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINNIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
BASE COUNT          361 a          220 c          201 g          371 t
ORIGIN      
        1 ggacaatcgc tttaggcgtt ccgcctaaac cactcactga ctcattcgtt acccttttga
       61 atctatttaa acttattatt accggtagaa ctttggtcgg ttctactggt ttatctcgat
      121 atccttaacg cactttacta ctctttagga gatcattttt ctatgactac atattatgaa
      181 gcaattaact ggaatgaaat cgaagacgtc attgacaagt caacttggga aaagttgacc
      241 gaacaatttt ggcttgatac ccgtatccct ttatcaaatg acttagacga ttggcgcaaa
      301 ctttctcttc aagaaaaaga tcttgttggc aaggtttttg gaggattaac cctacttgat
      361 accatgcaat cagaaactgg tgtcgaagct attcgtgctg atgttcgcac tcctcacgaa
      421 gaagctgtct taaacaatat tcaattcatg gaatctgttc acgctaaatc ttattcgtct
      481 atcttttcaa ccttaaacac caaaaaagaa attgaagaga tctttgagtg gactaataat
      541 aatgagtttc ttcaagaaaa agcacgtatt atcaataaca tttacgctaa tggagatgcc
      601 ttacaaaaga aagttgcctc aacatatctc gaaactttcc ttttttattc tggctttttc
      661 acccctcttt actatttggg aaataataag ctagcaaatg ttgctgaaat cataaaacta
      721 attattcgtg atgaatctgt acatggtact tatatcggtt acaaattcca gcttggtttt
      781 aacgaactac cagaagatga gcaagagaat tttcgtgatt ggatgtatga cctcctttat
      841 cagctgtatg aaaatgaaga aaaatacacc aaaacgcttt atgatggcgt agggtggact
      901 gaagaagtta tgaccttttt acgctacaat gctaacaaag ctcttatgaa tttaggacaa
      961 gatcctttat tcccagatac tgcaaacgat gttaacccaa ttgttatgaa tggtatttca
     1021 acaggaacat caaaccatga ctttttctca caggtaggga atggctatct tcttggttct
     1081 gtagaagcta tgtctgatga cgattataat tatggactct aatagtgctc acgagactga
     1141 taatctatca gtc
//
LOCUS       DAUELZ010000186         1143 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.187, whole genome shotgun sequence.
ACCESSION   DAUELZ010000186 DAUELZ010000000
VERSION     DAUELZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.187"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            36..>1143
                     /locus_tag="VHI09_001678"
     CDS             36..>1143
                     /locus_tag="VHI09_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEQ6121520.1"
                     /translation="MPSMKHILKSLSILLIGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVKISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKTVNSISDLQSS"
BASE COUNT          390 a          192 c          211 g          350 t
ORIGIN      
        1 tataagctat ggacaaataa ttcggaggac aaaacatgcc aagtatgaaa catatcttaa
       61 aatccttaag tattttacta attggatttt taggaggatt aatagcaatt attacattca
      121 ataatctcta cccacattct ccttcaaaaa taaattcagg taaggcaaca actagtaata
      181 tggtttttaa caacacaacc aatactacta aagctgtcaa agctgttcaa aatgcagttg
      241 tatcagttat taattatcaa gataaccctt cttcatccct ttctaaccct tatacaaaac
      301 tctttggaga agggcgttca aaagagaata aggatgctga attatctatt tttagtgaag
      361 gatctggggt catttatcga aaagatggca actccgctta cgttgttact aataaccatg
      421 ttatcgacgg agctaaacga attgaaattc ttatggcaga cggatctaaa gttgttggtg
      481 aattagttgg agctgatact tattcggatt tagctgttgt taagatctct tcagataaga
      541 taaaaacagt agctgaattt gcagattcta caaaactaaa tgttggagaa gttgctattg
      601 ctatcggcag cccactagga acacaatacg ctaattctgt tactcaagga atcgtctcta
      661 gtttaagtcg tactgtaact ttaaaaaacg agaatggtga gactgtctca acaaatgcta
      721 ttcagacaga tgctgctatt aaccctggaa actctggtgg accactaatt aatattgagg
      781 ggcaagtaat cggaattaat tcaagtaaaa tttcttctac cccaactggt agcaacggta
      841 atagtggtgc tgttgaagga attggttttg ctatcccatc tactgatgtt attaaaatta
      901 ttaaacaact agaaactaat ggtgaagtta tcagacctgc tcttggaata tccatggtca
      961 atttaaatga cttatccaca aatgctctta gccaaattaa tattccaact agtgtaactg
     1021 gtggtattgt agtagcagaa gttaaggaag gaatgccggc atctggcaaa cttgctcagt
     1081 acgatgtgat tacagaaatt gatggcaaaa cagttaattc aattagtgat ttacaaagta
     1141 gtc
//
LOCUS       DAUELZ010000187         1136 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.188, whole genome shotgun sequence.
ACCESSION   DAUELZ010000187 DAUELZ010000000
VERSION     DAUELZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.188"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..207)
                     /locus_tag="VHI09_001679"
     CDS             complement(<1..207)
                     /locus_tag="VHI09_001679"
                     /inference="COORDINATES: protein motif:HMM:NF014566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ6121521.1"
                     /translation="MEEILFRGILQEKLSRVSFWLSIIVTSVIFSYIHGNDTILNTQF
                     ISSLIYGMAYQTSKDLRFPIINHSL"
     gene            complement(564..740)
                     /locus_tag="VHI09_001680"
     CDS             complement(564..740)
                     /locus_tag="VHI09_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121522.1"
                     /translation="MGRLVGGNNHGYQKFFAWFSYWFTLPNFRQYIIEYPEKLEKPIE
                     DTKMGLYSVYIGDL"
     gene            complement(718..942)
                     /locus_tag="VHI09_001681"
     CDS             complement(718..942)
                     /locus_tag="VHI09_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEQ6121523.1"
                     /translation="MNTTIKQFEIMDEAMLTGIEGGCSWHGLAQAAVFTGAGNGFRLG
                     VKTRTWQGAAAGAAGGLVIGGVGYGATCWW"
BASE COUNT          397 a          226 c          145 g          368 t
ORIGIN      
        1 cagagaatgg ttaataatag gaaatcttaa atccttactt gtttgataag ccattccata
       61 tatcaaagaa ctgatgaatt gagtattcaa tatagtgtca tttccatgaa tatacgagaa
      121 tattacagat gtcacaatga tgcttaacca aaacgaaacg cgtgataact tttcttgcag
      181 tattcctcga aatagaatct cctccaagat tggttgagaa actacagtcg taaaaaaaat
      241 aatatgaact ttactggctg ataagacatt tcgctaagag tgacagaaaa taaaagcaaa
      301 gtattataaa aataatgcaa attattactc ttaaaaagtc tccattgatc ccttgcctaa
      361 gattaatccg ttcattgtta aatgtcgcaa caactggagt ttgaacaaaa atagtcataa
      421 atatagagaa tataattgac ataataaagg taagacactt cttaaagaca ccactcattc
      481 tatttgctcc tattttttaa catttaccag tatacttttt cttattccaa cgagacctat
      541 cacatccaag ctatgttaaa tccttaaaga tcacctatat aaactgagta aagccccatc
      601 ttagtgtctt ctatcggttt ttccaatttt tcaggatatt caataatata ttgtcgaaaa
      661 ttgggcaaag taaaccaata actaaaccaa gcaaaaaact tttgatatcc atggttatta
      721 ccaccaacaa gtcgccccat aaccgactcc tccaatgacc agtccaccag ctgctccagc
      781 tgcagctcct tgccaagtcc ttgttttaac acctaatcta aatccatttc cagcaccagt
      841 aaaaacagct gcctgagcta acccatgcca tgaacaacca ccttcaatac cagtaagcat
      901 ggcctcatcc ataatttcaa attgtttaat tgttgtattc ataacgaata tctccttata
      961 taatttttta ttatgtcttg ttgcaacctt gacatgttca gtatagcatc ttaagaaatc
     1021 tgtgccccgt aaaatcttca aatgttcttt tttcatcatg aaatgatttt tttcatagtt
     1081 ttttaatcgt ttcgaattag aaaataatca ctagaaactt attactttta atttta
//
LOCUS       DAUELZ010000188         1107 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.189, whole genome shotgun sequence.
ACCESSION   DAUELZ010000188 DAUELZ010000000
VERSION     DAUELZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.189"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..>1107
                     /locus_tag="VHI09_001682"
     CDS             <1..>1107
                     /locus_tag="VHI09_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEQ6121524.1"
                     /translation="RKKYNLTQKELADALGLQKFGDRTIRRWEAEETHPSKLEQSSIQ
                     SFFNSLKNPPFIISENLPFKMIDLFAGIGGTRLGFQLTNEVETVFTSEWDKFAQKTYI
                     TNFGDTPEGDITQIDENTIPDHDILVAGFPCQAFSQAGKRLGFEDTRGTLFFDVARII
                     KAKRPKAFLLENVKNLKTHDKGKTYKIIISTLENLGYSTSTVLFKARDFGVPQNRERI
                     YIVGFDKTCVNNFSEFRFPEPFHLETKLGNILQTDVDEKYTISDKLWEGHQRRKAEHK
                     TKGNGFGYSLFNADSPYTNTLSARYYKDGSEILIEQKGKNPRKITPREAARLQGFPEE
                     FIIPVSDTQAYKQFGNSVCVPTIHEIAKQILAVL"
BASE COUNT          411 a          188 c          186 g          322 t
ORIGIN      
        1 acgaaaaaaa tataatctca ctcaaaaaga attagcagat gcactcggtc ttcaaaaatt
       61 tggagataga accattagac gttgggaagc agaagaaacg catccttcaa aacttgaaca
      121 gtccagtatt cagtcttttt ttaattctct aaagaatccg ccttttatta ttagtgaaaa
      181 tcttccgttc aaaatgatcg atctttttgc tggaataggt ggaactagat taggtttcca
      241 attaacaaat gaagtagaaa ctgtatttac aagtgaatgg gataaatttg ctcaaaaaac
      301 atacattact aattttggag acactccaga aggtgacatc actcagattg atgaaaatac
      361 tataccagat catgatatac tcgttgctgg ttttccttgt caagctttct cccaagcagg
      421 taaacgtcta ggatttgaag atactcgtgg aactttattt tttgacgttg ctagaataat
      481 aaaagctaaa cgtccaaaag cttttttact tgaaaatgtc aaaaacctaa agacacatga
      541 taaaggtaag acatacaaga taatcatttc aactcttgag aatttaggat atagtacatc
      601 aactgtactt tttaaggcca gagattttgg tgtaccacaa aatagagaaa ggatttatat
      661 agttggattt gataagactt gtgtaaataa ttttagtgaa ttccgtttcc ctgaaccgtt
      721 tcatttagaa actaaactag gaaatattct acaaactgat gttgatgaaa agtatactat
      781 ctccgataaa ctctgggaag gacaccaaag aaggaaagct gaacataaaa caaaaggaaa
      841 tggattcggc tactcactat ttaatgctga tagtccatat acaaatacac tttctgctcg
      901 atactacaaa gatggaagtg aaatattaat agaacaaaaa ggtaaaaacc cacgtaaaat
      961 tactccacga gaagctgcta gactccaagg tttccctgaa gagtttataa ttccggtttc
     1021 tgatacacaa gcctacaaac aatttggaaa ttctgtctgt gtaccaacaa ttcatgaaat
     1081 tgctaaacaa atactagccg tcctaaa
//
LOCUS       DAUELZ010000189         1107 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.190, whole genome shotgun sequence.
ACCESSION   DAUELZ010000189 DAUELZ010000000
VERSION     DAUELZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.190"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(1..1039)
                     /locus_tag="VHI09_001683"
                     /pseudo
     CDS             complement(1..1039)
                     /locus_tag="VHI09_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
BASE COUNT          375 a          187 c          193 g          352 t
ORIGIN      
        1 tacttactta acttaaaaga tttatccaaa tagtctacaa attcactatc gattttctga
       61 taacgaattt cctcgctagt caatgatttg attgtttcaa taccattaat atcttcgata
      121 atagcagagc tggccatgga attactctgc atgacattgt tattcatctt ttcaaaaggt
      181 ttcataaaag taaaaatgat gataatgtag attgggatag aaattaatga tagaagaaat
      241 aggttagtgt tttgtacgag taacacacca cctacaatac ttaaaataga gacatctaaa
      301 aatagagata gaattgtaga agcaagtgta tcaataatag cattagcatc tgtaaaacgt
      361 gagatgattt ctcctgtccg acgtgttaca aagaaagaca tgggaagctc aaaaatatga
      421 cgaatatagg ataaaataac atcaatgctt aatcgctgac tcagtacagt caggagataa
      481 tctcgcgaaa aactcatcat ctgttgtagg atataggtaa cgataagtcc aattgagatt
      541 ataccgagtg ttggcttcat ctgatttgga atatattcat cccaaatacc ttgtagatag
      601 taggaaccac caatatttat cagggtaact aagaggctcg ctaaaacgat atagaagatt
      661 aaggactctt gtttaaaaat tagaggtaga aaactcatta agccattctt tttatccttg
      721 tgaggtttat agccaggttc aggtgctaaa aagatagcaa cccctgtcta ttcaggagca
      781 aatttttctt tgctcatttt ggttacttta acggagggat ctggatctcc aatgatgaaa
      841 tgttcttttc aaagcccatc tccttggccg cttttacgat tccaagggtt gttgttcctt
      901 ctttattagt ctttgccaat tctctcagat gtgtcaaaga gtagtcagac ccgtagaatt
      961 ttgcaatcga agctagagca gcaattccac aatctcttgc atcaatttgt ggtacaaacg
     1021 tacgtttaca tgatgtcatt aatatatttc cttccagtaa attacaaatt cattattgca
     1081 aaactactga taagaaattc gaaaatt
//
LOCUS       DAUELZ010000190         1092 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.191, whole genome shotgun sequence.
ACCESSION   DAUELZ010000190 DAUELZ010000000
VERSION     DAUELZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.191"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..1074)
                     /locus_tag="VHI09_001684"
     CDS             complement(<1..1074)
                     /locus_tag="VHI09_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ6121525.1"
                     /translation="MFDFANNTFLAGLNPASLSLVAVYQSLESVIGVLFNLFGGVIAD
                     SFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAIVLTNVILAFMSAFSSPSYKAFTK
                     EIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYKLLGIHGVLLLDGLSFLIAALLIS
                     FILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHKSIFIITVLSALVNFFLAAYNLLL
                     PYSNQMFGEISTGLYGTFLTAEAIGGFIGAILSGFVNKELSSMRLILFLSLSGLMLML
                     APPFYIMFHNAIILALSPALFSLFLSIFNIQFFSLVQKDVDNDFLGRVFGIIFTITIL
                     FMPIGTGFFSVALNPNNSFNLFII"
BASE COUNT          436 a          179 c          160 g          317 t
ORIGIN      
        1 aataataaaa agattaaagc tattgttagg atttaatgct accgagaaaa agccagtccc
       61 aataggcata aaaagaatag ttattgtaaa gataatacca aacactctcc ctaaaaaatc
      121 gttatcaaca tctttctgga caagagaaaa aaattgaata ttaaagatgg aaagaaataa
      181 actgaataaa gctggtgaca aagctaaaat aattgcatta tgaaacataa tataaaatgg
      241 gggagctaac attaacatca gtcctgataa agatagaaac aatatcaatc tcatacttga
      301 taattcttta ttcacaaaac cacttaatat tgccccaata aatccaccaa ttgcttctgc
      361 tgttaaaaaa gtgccataaa gtccagttga aatttctcca aacatttgat tactataggg
      421 caacaataaa ttataagcgg ctagaaaaaa attaacaagt gcagaaagaa cggtaataat
      481 aaagatggac ttatggctgt aaacatattt aaatcctatt tttaaatcgt taaatatttc
      541 tctgattgtc accttctcct ttatcaccac ttcgtcatta acaggtaaaa taaaggaaat
      601 tagtaaagca gctattagaa atgatagtcc atccaatagt aatacaccgt gtataccaag
      661 tagcttatat aagaaaattg ctaccatagg aactgttact ttgattacag tacttgttgt
      721 ctctagcaat gaattaagtt gtgatatact atctttttta acaatttctt ttgtaaatgc
      781 tttataggat ggactagaaa aagcactcat aaatgccaag ataacattag ttagtacaat
      841 ggcataaacc aaccattgct cttttgttag gaacgagagt actaagcaag ctgtgccaca
      901 taagatattt gtagtaataa taattttttt ccgcttgaaa ctgtctgcaa tgactccacc
      961 aaataaatta aatagaacac ctatgacact ttctagagac tgatatactg caactaatga
     1021 tagagacgct ggattaagcc cagcaagaaa agtattgtta gcaaaatcaa acataacatc
     1081 gccaattcga ta
//
LOCUS       DAUELZ010000191         1022 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.192, whole genome shotgun sequence.
ACCESSION   DAUELZ010000191 DAUELZ010000000
VERSION     DAUELZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.192"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(345..>1022)
                     /locus_tag="VHI09_001685"
     CDS             complement(345..>1022)
                     /locus_tag="VHI09_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LlaJI family restriction endonuclease"
                     /protein_id="HEQ6121526.1"
                     /translation="KKMATTFNDMERRLFQSMIDILNYKDRQEEPDKFYFGTNNFQYI
                     WENLIDYTYGIANKTSYFPRTKWDLVFETKTSKGYALEPDTIMVHNDNVFVLDAKYYK
                     FGQTNLMKDLPPSTSINKQITYGEYVANQDLLKKLHGQNSKVYNAFLMPFSKSHEIFQ
                     TDGMYQYIGEALGEWKGQDKTYERVQGILVDVKTLVHNSARNRTQYIQELSDLISEKV
                     DEKLANM"
BASE COUNT          352 a          175 c          132 g          363 t
ORIGIN      
        1 gtgaattgaa aaatgcacta ttaatcttgt caaaggaaat acttgcactg gaaattaata
       61 gaggattagc taaagtccaa cctaaaataa atttagatac tttactacct gattggaatt
      121 taccagattt aatttcagca ttttacttca ctttatttta ctcaaatcct aaaattgcca
      181 tgtataaaat ttgcgagaat ataggatgta acaccccatt ttatgtccaa aggtcaaata
      241 caataaaaaa atattgctct gaaagttgta agaacgcttc ttctcagaga agatatagaa
      301 ataaacaaaa agactttcag tgaaaattat cactgaaagt cttattacat atttgctaat
      361 ttttcatcaa ccttctcgga gattaaatcg gataattctt ggatatactg agtccgattt
      421 ctagcagaat tatgtaccaa agtttttaca tcgactaaaa taccttgaac tcgctcataa
      481 gttttatcct gacccttcca ctcaccaaga gcttctccga tatactgata cattccatct
      541 gtttgaaaaa tttcgtgact ctttgaaaat ggcattaaga atgcattata aacttttgaa
      601 ttctgtccat gtaatttttt aagcaaatct tgatttgcca catactcacc atatgtaatt
      661 tgcttattaa tacttgtaga aggaggcaaa tctttcatta agttagtttg accaaacttg
      721 taatattttg catctaatac aaaaacattg tcattatgaa ccataatagt atccggttct
      781 aatgcatacc cttttgaagt ttttgtctca aaaactaaat cccattttgt tctaggaaaa
      841 tatgaagttt tattggcaat tccatatgta taatctataa gattttccca gatatattgg
      901 aaattattag ttccaaaata aaacttatca ggttcctctt gcctatcctt ataattaagt
      961 atgtcaatca tactttggaa aagtcttctc tccatatcat taaaagttgt agccatcttt
     1021 tt
//
LOCUS       DAUELZ010000192         1022 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.193, whole genome shotgun sequence.
ACCESSION   DAUELZ010000192 DAUELZ010000000
VERSION     DAUELZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.193"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            178..>1022
                     /locus_tag="VHI09_001686"
     CDS             178..>1022
                     /locus_tag="VHI09_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEQ6121527.1"
                     /translation="MFGKQGIRCARKGDERNIKIIVNRVNSERNIKSIGICSPSYPGT
                     FHYQKRYKRGVEFLQNKGFTIVEGNLTGKEGYRGEISGGVQDRAEELNQLIPRVDVIM
                     TSIGGYNSNSILKYIDYDLFKQKSPIFIGYSDTTALALALYNKTGCITYLSQSVISNF
                     GEFEPFNELNYFYFDFMLQSKCETLMVQIPDVWTDEWINWETYEKTKKTNKNEWIIFN
                     KGEFNGTLIGGNLDTIVGIIGTEYMPKITEDTILLLEDVYTDLGRLYRNFTTLALHGI
                     FDKIGG"
BASE COUNT          380 a          131 c          205 g          306 t
ORIGIN      
        1 taagttttca ggaattgtat ttttcagact caatccagag aattacggaa aaatatttaa
       61 tggagtgagt tacgatgcac ctaaaacttc actaatattt gaagaaaaga tttataaaga
      121 ggaaggaact gtattgccaa atccgattaa ttggagtaat gagagagtag gatatgtttg
      181 tttggaaaac aaggaatacg ctgtgctaga aaaggagatg aaagaaatat taaaatcatt
      241 gtcaatagag taaatagtga aagaaacatc aaatctatag gtatttgttc accctcctat
      301 ccggggacat ttcattatca gaaacgatat aagagaggag tagaattttt acaaaataaa
      361 ggatttacta tagtagaggg gaatttaaca ggaaaagaag gttatagagg ggaaatttca
      421 ggaggtgttc aagacagagc tgaagaatta aatcaactta ttccgcgtgt ggatgtcatc
      481 atgacttcca taggtggcta caattctaat tctatattaa agtatataga ctacgatcta
      541 tttaaacaga aatctcctat ctttattgga tattctgaca caacagctct tgctttagcc
      601 ttatataata agactggatg tataacttac ctgtctcaga gcgttatctc taattttgga
      661 gagtttgaac cgtttaatga attaaattat ttctattttg acttcatgct tcagagtaag
      721 tgcgaaacct taatggtaca aataccagat gtttggacgg atgagtggat aaattgggaa
      781 acctatgaaa aaaccaaaaa aacaaataaa aatgagtgga ttatttttaa caaaggtgaa
      841 tttaacggta ctttgatcgg aggaaatctc gataccattg taggaataat aggaactgag
      901 tatatgccaa aaataacaga ggataccata ctcttgttag aagatgtata tacggatcta
      961 ggaagattat ataggaattt cacaacgctt gccttacatg gtatttttga taaaatagga
     1021 gg
//
LOCUS       DAUELZ010000193          972 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.194, whole genome shotgun sequence.
ACCESSION   DAUELZ010000193 DAUELZ010000000
VERSION     DAUELZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.194"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            195..>972
                     /locus_tag="VHI09_001687"
     CDS             195..>972
                     /locus_tag="VHI09_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ6121528.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQL"
BASE COUNT          332 a          139 c          185 g          316 t
ORIGIN      
        1 cttgcagaaa aagtcaagcc tttttttaaa aaaaagtcgt ttttttaaaa aaatagttta
       61 gagaatttga gttaaaatga caagggaatc ttgattgaag ttgatttttt gaaagataat
      121 tgtataatgg aaacgaaata catgacaaac ccatgataag gtttatcttt ggtaaatata
      181 gtaacgaggt taatatggta acaataaaag atgtcgctca aaaggcaggt gttaaccctt
      241 cgacggtgag tagggtatta aaagataatc gatctatttc tatgaaaaca aaagagaagg
      301 ttagaaaagc gatggcagat ttgggatatg ttccaaatgt cgcagcacag attctagcga
      361 gtggtttgac acacaatata ggacttgttt ttcctcctat tacaactagc gatagattaa
      421 gtgaaccttt ctttatggaa attttgtcga ctattactaa cgaagctaaa gaatctcatt
      481 ttactgtgtc gattgctaca ggtatatctc ttgatgacct gatagagcaa gtgaaactga
      541 tgcatcttca aaaacgagtt gatggtttta ttattcttta ttctgatcaa gatgatcctg
      601 tcaaaaaata tttgatgaca aataacttac catttgtcat tgtaggtgct ccacaaggtg
      661 acactaataa aaccacttat attgataatg ataatcagct aatggctaaa acagcagtag
      721 aacaccttta tcaaaaaggc catcacaaca tattatttat aacagatgat ttactatcag
      781 aagtaacttc tgagcgttat cttggctatt tgaagggttg ttttaagcga tctttaaaaa
      841 caaagccaat gctgttattt gatcgtaagg atcctgttag cgtagaaata ttgatggaaa
      901 ctatatcttc ctttaaagca actgccttgg ttgttgttgg tgatgttctg gctattcgta
      961 tggtacagtt at
//
LOCUS       DAUELZ010000194          965 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.195, whole genome shotgun sequence.
ACCESSION   DAUELZ010000194 DAUELZ010000000
VERSION     DAUELZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.195"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            54..791
                     /locus_tag="VHI09_001688"
     CDS             54..791
                     /locus_tag="VHI09_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121529.1"
                     /translation="MKQLVLKDVCKKYPNQLNYALDHINLTVRKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGNIYCADKEISVFSDNEATSFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNIDSSKIDDLINSYAKRFNLYEQLKKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEETMMILSEINKTEKVTILMVTHDVLSASYADRVVLLKDGK
                     LHMEIDKKDCGESFYDVISQALSDRGE"
BASE COUNT          340 a          117 c          185 g          323 t
ORIGIN      
        1 tattgtgact tatatgactc gtatatgaat aaatatcagg aggaagaggc aaaatgaaac
       61 aattagtatt aaaagatgtt tgcaaaaaat atcccaatca actgaattat gctctagacc
      121 atatcaattt gactgttaga aaaggagaat ttgtagctgt catggggcgt agcggtagtg
      181 ggaaaacgac gcttttaaat gttacttcaa ttattgataa aattgatagt gggaatattt
      241 attgtgcgga taaagagatt agtgtatttt ctgataacga ggcaactagt tttagaaaaa
      301 atgatattgg ttttgttttt caagattata tgctgttaga cagtctaacc attagggaga
      361 atatttctgt tgctttgtct ctaaaaaata ttgattcttc aaagattgat gatttgataa
      421 atagctatgc aaaaagattt aatttatatg aacagttgaa gaaatatccg tatcaacttt
      481 ctggaggtca aagacagagg gtcagtatta tcagagcaat cattaaagaa ccagagatta
      541 tttttgctga cgaaccaact ggagcacttg acttgaaatc ttctgaggaa acaatgatga
      601 ttttgagtga aatcaataaa acagaaaagg taactatttt aatggttact catgacgttt
      661 tatcagcttc ttatgccgac agagttgtac ttctcaaaga tgggaaactt cacatggaga
      721 ttgataaaaa agattgtgga gagtcgtttt acgatgtaat tagtcaagct ttatccgata
      781 gaggagagta atatgatttg gtccattaca aaatctaaca ttaaaaaaaa tttttcgtta
      841 tatcgtatct attttctagc tacgattggt ttattaagta tttttatagc ttttctaaat
      901 tttatctcag ataaaatcat tacagaaaaa attggggata gtggtcaagc tctagttatc
      961 gctaa
//
LOCUS       DAUELZ010000195          960 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.196, whole genome shotgun sequence.
ACCESSION   DAUELZ010000195 DAUELZ010000000
VERSION     DAUELZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.196"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(78..209)
                     /locus_tag="VHI09_001689"
     CDS             complement(78..209)
                     /locus_tag="VHI09_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEQ6121530.1"
                     /translation="MTIKNQKKYKGVYCDKNGKIFYQADLGVDPVTGKRVQKKARKN"
     gene            complement(212..433)
                     /locus_tag="VHI09_001690"
     CDS             complement(212..433)
                     /locus_tag="VHI09_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEQ6121531.1"
                     /translation="MNIGFLEHTARDIIREAKRIAIKKFEEAREIDQNAVQLSKSPFD
                     NRRLGIAPKDIVEELIGILLSEESLESEN"
     gene            520..663
                     /locus_tag="VHI09_001691"
     CDS             520..663
                     /locus_tag="VHI09_001691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121532.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            complement(734..952)
                     /locus_tag="VHI09_001692"
     CDS             complement(734..952)
                     /locus_tag="VHI09_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6121533.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFSKLYNVSIDYLLGQTDYPYRFHNK"
BASE COUNT          284 a          210 c          130 g          336 t
ORIGIN      
        1 agattattac taaacccata cctaatccga accagttcat catacgcttc tttcattgtg
       61 ccaaatggct ctccatacta attctttctg gctttctttt gaactcgttt acctgttact
      121 ggatctacac caagatcagc ttgataaaat atcttaccat tcttatcaca ataaacacct
      181 ttgtactttt tttgattttt tatagtcatc tttaattctc actttctaaa ctttcttcag
      241 agagtagaat acctattaac tcttctacaa tatctttagg agcaatacca agtcttctat
      301 tgtcaaaggg cgatttactt aattgtaccg cattttggtc aatttcgcga gcttcttcaa
      361 acttttttat agctattctt tttgcttctc taataatatc tcttgcagta tgttcgagaa
      421 aaccaatatt catcaagctt tttatatgtt actattttca cacctccact cctttcaaaa
      481 aaattgcaac ctccgtgggt atgactggtt aaatcactca tgggaatctc acccactggc
      541 cttttacgct ggcagcctga acgttcagaa gtatcattgt ccctatttgt ggtcatggca
      601 gatagagaac cactctattt tatagctaat aattatcgct cctatcacgg cgtatctcac
      661 taaattgctc gacaaacaag aaagtcccac ctcggtttat tcagttgtca atgaacaaat
      721 caaggcgaga cttttattta ttatgaaaac gatacggata atctgtttgc cctaataaat
      781 aatcaatact gacgttatag agtttagaaa actgcacaag aacatctgcc attaaggcaa
      841 cctctcctct ctcaattttt gcataatttg catgagagaa ggaaagtaaa tttgcaacaa
      901 acttttgagt gaaatcgtta tcttctctca aattcctaat acgcggatac atctaacatt
//
LOCUS       DAUELZ010000196          953 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.197, whole genome shotgun sequence.
ACCESSION   DAUELZ010000196 DAUELZ010000000
VERSION     DAUELZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.197"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            93..932
                     /locus_tag="VHI09_001693"
     CDS             93..932
                     /locus_tag="VHI09_001693"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEQ6121534.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
BASE COUNT          312 a          140 c          181 g          320 t
ORIGIN      
        1 catgaagttt ttcgaatgtt tatttattct ggtatttttt ggtaaaatta gttagaatag
       61 tatgaagtat gcttacagaa aggttattag agatgacaaa agctgaccag atttttaaag
      121 ctaatattca aaaaattata aatgaaggat cattgagtga gcaagcacgt cctaaatata
      181 aagatggtcg aacagctcat tctaagtata ttactggggc gtttgctgaa tatgatttag
      241 ctaaaggaga gtttcccatc acaactttac gacctattcc aattaaatct gctataaagg
      301 aattattttg gatatatcaa gatcagtcta acagtttaga tgtgctagaa gctaaatata
      361 atgttcatta ttggaatgaa tgggaagttg accagacacg tactattggg caacgttatg
      421 gtgctgtagt caaaaaacat gatatcatct caaaaatact aaagcaatta gcagaaaatc
      481 cttggaatcg tcgtaatgtc atttctctct gggactatga agcatttgag gagacaaaag
      541 ggttgttgcc atgtgccttt caaatcatgt ttgatgttag acgtgtaggg gaagatcttt
      601 atttagatgc tagtttgacg caacgttcta atgacatatt agttgctcat cacatcaatg
      661 ccatgcaata cgttgccttg cagatgatga ttgctaaaca ttttggatgg aaaataggta
      721 agttctttta ttttgttaac aatttacata tttatgacaa ccaatttgat caggctcaag
      781 aattactaaa acgacagcca gttgccagtc agcctaagct tgttttaaat gttccagatg
      841 ggactaattt cttcgatatt aaaccagatg attttgaatt acaaaattat gatccagtga
      901 agcctcagtt gcactttgac cttgctattt aaactttatt tagatttttt gtg
//
LOCUS       DAUELZ010000197          944 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.198, whole genome shotgun sequence.
ACCESSION   DAUELZ010000197 DAUELZ010000000
VERSION     DAUELZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.198"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            118..399
                     /locus_tag="VHI09_001694"
     CDS             118..399
                     /locus_tag="VHI09_001694"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ6121535.1"
                     /translation="MTVLNYVNAGDAKSIHNHLFHYTRQVRRHVKVITIGILSAHYYE
                     ITMLFLQAKIILDCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(850..937)
                     /locus_tag="VHI09_001695"
     tRNA            complement(850..937)
                     /locus_tag="VHI09_001695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(901..903),aa:Ser,seq:gct)
BASE COUNT          337 a          199 c          111 g          297 t
ORIGIN      
        1 tgtaaaaagt tccatatgac gatctataat gaaaagcgac caaactatca tttcagaaag
       61 actcatataa aatcatccga ttatagtata atttcactag gaaactaaaa ccttagcatc
      121 acggttttaa attatgtaaa tgctggggac gcaaaaagca ttcataacca tctctttcat
      181 tataccagac aggttagaag gcatgtcaaa gttattacca taggcatact cagtgcccac
      241 tactatgaga ttactatgct ttttctacag gctaaaatta tcctcgattg ttttcatatc
      301 ccccaacgtc ttagacaagc tataactcgc tttcgtatcc gaatcataaa tacttctgct
      361 ataaaaccta tcgcgtatag aactctcaaa ctttactgaa aactcataca gcatgattca
      421 cggaagctca tcaataatag ctttattgcc caatttttcg tacgcccata actaatatct
      481 agaaaagaaa agctcttact cacaagatct ctgaatttca ataattattc ctaactctat
      541 caactcttac tcttcccatt tttcagacaa tttttaaaat ttttctaaaa gaaaagaaca
      601 agattagcaa tatacttaaa ctcaactact ctaaggccaa attagaaaca gtcaacaacc
      661 ttatcaaagc catcaagtta aacgttttac tcctcttagt tatcgaaata atgaacgatt
      721 taaatattct atttagttgg ttttgaagat tacaaaaaaa aagacgataa ccgtcttctt
      781 tcccctttat ttataattca tctattaata tgtttcattt aacatattaa ccaaaatatg
      841 tttacaaaac ggagaataag ggattcgaac ccttgcgcca gttacccgac ctaacgattt
      901 agcaaaccgt cctcttcagc ctcttgagta attctccaaa tata
//
LOCUS       DAUELZ010000198          936 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.199, whole genome shotgun sequence.
ACCESSION   DAUELZ010000198 DAUELZ010000000
VERSION     DAUELZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.199"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(34..720)
                     /locus_tag="VHI09_001696"
                     /pseudo
     CDS             complement(34..720)
                     /locus_tag="VHI09_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(811..885)
                     /locus_tag="VHI09_001697"
                     /pseudo
     CDS             complement(811..885)
                     /locus_tag="VHI09_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
BASE COUNT          279 a          176 c          160 g          321 t
ORIGIN      
        1 gttgtgtaaa cacaagagta gagtttttga atattagtca aataagctat caagtgtgtc
       61 aaaacatact ccgaatcctt tatgaatacg agctccgaac ttaatattat aatcttcgaa
      121 aatacttaca agacatcgtt ctaaggcttc ttcatttgga aaaaccaccc tccttcttgc
      181 tttgtcgctt aatttcttta ttgagtgatt caatcaagtt tgtagagtaa atactagacc
      241 aaatgtaatg tggaaattga tagaaagtta ggagattttc cgttgtttca agtgatgtca
      301 taaccttttt gtaacgaggc ttccactcag aaacaagtgg tgaccataca tgcgctcaat
      361 aatgtcactg atttcgcgtg tcgtaacgcc agtctgatag agtttgatca ccatctcttc
      421 caagtggtca tctcgtctag cataagatgg taataaaact ggtgaaaact cgccgtttcg
      481 atctcttggg atgattaaat tgactaagcc atactttgtt tcaaactgac gagaataagt
      541 cccattacgg ctattacctg tgttgtaacc ttctttttca taaggttcat aaccaagaaa
      601 agctgatagt tcaacttgca agaggtcatt catagctatt tctaaagagg agcgaaaaaa
      661 ttcatcaata tcttgttttt gtgctaggaa gttaagtaat tctgtggtaa actgagtcat
      721 gggaataaat ctctttctag tgaagtgtcg caactctact atacaggatt tattcctttt
      781 tgtgtgttta cacaagatat tttacactac ccaattacca ccctcaacct tggcaagctt
      841 ttctatagtc atttggtgaa atgtttccag tttctttatc tccatatcga ataacttctt
      901 ttcataattc ttaaatttgt ctcgttgcaa cctcga
//
LOCUS       DAUELZ010000199          926 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.200, whole genome shotgun sequence.
ACCESSION   DAUELZ010000199 DAUELZ010000000
VERSION     DAUELZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.200"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..858
                     /locus_tag="VHI09_001698"
     CDS             <1..858
                     /locus_tag="VHI09_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ6121536.1"
                     /translation="TDDLKPFQVKDLVSGHLERIQEEVEKKYPDAGFNSDKYNGLKES
                     NFLLSDGFEVKSGNLIFDKKLTIPLTTLFDVINPDFLANSDRAAYDNYRTYKEQHPKK
                     LVALTFDDGPDPTTTPQVLDILAKYQAKGTFFMIGSKVVNNENLTKRVSDAGHEIANH
                     TWDHPNLTNLSVSEIQHQVNMTNQAIEKACGKKPRYLRPPYGATNATVQQSSGLTQML
                     WTVDTRDWENHSTDGIMTNVKNQLQPGGVVLMHDIHQTTINALPTVMEYLKAEGYECV
                     TVSELYAHQ"
BASE COUNT          327 a          153 c          177 g          269 t
ORIGIN      
        1 acagacgact tgaaaccgtt tcaggtaaag gatctagtat caggacattt agaaagaata
       61 caagaagaag ttgaaaaaaa atatccagat gctggtttta atagcgataa gtataatggc
      121 ttaaaagaat ctaatttttt attaagcgat ggctttgagg taaaatcggg aaaccttatt
      181 tttgataaaa agctaacgat acctttgacg acattatttg atgttattaa tccagatttt
      241 ttagcaaata gcgatagagc tgcgtatgat aattatagga cctacaaaga acagcatccc
      301 aaaaaactag ttgcattaac gtttgatgat ggtccagatc cgacgacgac tcctcaagtt
      361 ttagatattt tggcaaaata ccaggctaag ggaactttct ttatgatagg ttcaaaagtt
      421 gtgaataatg aaaatcttac taaacgtgtt agcgacgctg gccatgaaat tgctaatcat
      481 acttgggatc atcccaatct gactaatctt tcagtcagcg agattcaaca tcaagttaat
      541 atgacgaacc aggctattga aaaagcttgt ggcaagaaac ctcgctattt acgccctcca
      601 tacggagcta caaatgcaac tgttcagcaa tcatcaggat taacacaaat gctttggacg
      661 gttgatacaa gagattggga aaatcatagc actgatggca ttatgactaa tgtcaaaaac
      721 caattgcaac cgggaggagt tgttttaatg catgatattc atcaaacaac tatcaatgct
      781 ttgccgacag ttatggaata tctaaaagca gaaggatacg aatgtgtgac tgtatcagaa
      841 ctctatgcgc atcagtaagt aatatcgtta tgtttcttta gaattgatat actaaacaat
      901 ccaattactt ttgaagtaat tggatt
//
LOCUS       DAUELZ010000200          900 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.201, whole genome shotgun sequence.
ACCESSION   DAUELZ010000200 DAUELZ010000000
VERSION     DAUELZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.201"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(19..92)
                     /locus_tag="VHI09_001699"
     tRNA            complement(19..92)
                     /locus_tag="VHI09_001699"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Ile,seq:cat)
     gene            complement(113..186)
                     /locus_tag="VHI09_001700"
     tRNA            complement(113..186)
                     /locus_tag="VHI09_001700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Met,seq:cat)
     gene            complement(192..265)
                     /locus_tag="VHI09_001701"
     tRNA            complement(192..265)
                     /locus_tag="VHI09_001701"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Pro,seq:tgg)
     gene            complement(272..345)
                     /locus_tag="VHI09_001702"
     tRNA            complement(272..345)
                     /locus_tag="VHI09_001702"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(309..311),aa:Arg,seq:acg)
     gene            complement(362..446)
                     /locus_tag="VHI09_001703"
     tRNA            complement(362..446)
                     /locus_tag="VHI09_001703"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Leu,seq:taa)
     gene            complement(455..526)
                     /locus_tag="VHI09_001704"
     tRNA            complement(455..526)
                     /locus_tag="VHI09_001704"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(492..494),aa:Gly,seq:gcc)
     gene            complement(539..611)
                     /locus_tag="VHI09_001705"
     tRNA            complement(539..611)
                     /locus_tag="VHI09_001705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(576..578),aa:Thr,seq:tgt)
     gene            complement(622..703)
                     /locus_tag="VHI09_001706"
     tRNA            complement(622..703)
                     /locus_tag="VHI09_001706"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(667..669),aa:Leu,seq:tag)
     gene            complement(709..781)
                     /locus_tag="VHI09_001707"
     tRNA            complement(709..781)
                     /locus_tag="VHI09_001707"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(746..748),aa:Lys,seq:ttt)
     gene            complement(813..885)
                     /locus_tag="VHI09_001708"
     tRNA            complement(813..885)
                     /locus_tag="VHI09_001708"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(850..852),aa:Asp,seq:gtc)
BASE COUNT          241 a          261 c          197 g          201 t
ORIGIN      
        1 taatgattat ctatgatatg gaccttgtag gactcgaacc tacgaccgct cggttatgag
       61 ccgagtgctc taaccagttg agctaaaggt cccaagtcat ccataaaaat aatagcggcg
      121 aaggggatcg aacccccgac ctcccgggta tgaaccggac gctctagcca gctgagctac
      181 accgccatta atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta
      241 ctctaccaag ctgagctact tcccgaactg atgcacccta gaggagtcga acctctaacc
      301 gcctgattcg tagtcaggta ctctatccag ttgagctaag ggtgctattt caaaaaatta
      361 atgccgagga ccggaatcga accggtacga tgtaaaccat cgcaggattt taagtcctgt
      421 gcgtctgcca gttccgccac cccggctcct caaaagcgaa cgacgggatt cgaacccgcg
      481 acccccacct tggcaaggtg atgttctacc actgaactac gttcgcaact aatctcaatg
      541 ccggctacat gacttgaaca cgcgaccctc tgattacaaa tcagatgctc taccaactga
      601 gctaagccgg ctaatctttt atgcgggtta agggacttga acccccacgc cgttaagcgc
      661 cagatcctaa atctggtgcg tctgccaatt ccgccaaacc cgcttttatg acccgtactg
      721 ggctcgaacc agtgacccat tgattaaaag tcaattgctc taccaactga gctaacgagt
      781 ctttccttca aaaaatatga ctactctttg aacggtcccg acgggaatcg aacccgcgat
      841 cttcgccgtg acagggcgac gtgataaccg ctacactacg ggacctgtat tttccaatac
//
LOCUS       DAUELZ010000201          864 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.202, whole genome shotgun sequence.
ACCESSION   DAUELZ010000201 DAUELZ010000000
VERSION     DAUELZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.202"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..>864)
                     /locus_tag="VHI09_001709"
     CDS             complement(<1..>864)
                     /locus_tag="VHI09_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEQ6121537.1"
                     /translation="AFNYDFSHLRKKKLNQRTRKIISIANFSMILYFITIPMIDFIEE
                     VENINFTHYQEMITAIYFLLFICFVNLLDRNLRQELQKQVVLQKELQLQNMARYSKQI
                     EGLYQNIRSFRHDYANILTSLKIGIDCKDIEMISSIYHKVLKDSGKVLRGEKFNLARL
                     RNINDLPLKSLLFVKLSEAQRLSIPVSIEIGEPISIKNIELIDFLTLVSILIDNAIEG
                     AAESGIVLCFFEQFGKQILIVQNDTFEKQINLSTIFERGVSSKGNNRGVGLSNVREIL
                     ESYPTVTLKTV"
BASE COUNT          287 a          148 c          115 g          314 t
ORIGIN      
        1 ttactgtttt gagggtaaca gtgggatatg attctaatat ctccctgaca tttgataaac
       61 caactcccct attatttcct tttgaagaaa cacctcgctc aaaaatcgtg gaaagattaa
      121 tctgtttttc aaaagtatca ttttgaacaa ttaatatctg tttaccaaat tgttcaaaaa
      181 aacaaagaac tatccccgac tctgctgcac cttcgattgc attatctatc aagatagaaa
      241 caagggttaa aaaatcgatt aactcaatgt tttttataga aatcggttcc ccaatttcta
      301 tagatacagg tatggataag cgttgagctt ctgataattt tacaaacagt aaacttttca
      361 gaggaaggtc gtttatattt ctcagccttg ctagattgaa tttttctccc cttaaaacct
      421 tccctgaatc tttcaacacc ttatgataaa tgcttgaaat catttcaata tctttgcaat
      481 caatgcctat tttcaagctt gttaaaatat ttgcataatc atgtctaaaa cttctgatat
      541 tttgatacag tccttcgatt tgcttactat agcgagccat attctgtaat tgtaactctt
      601 tttgtagaac aacttgcttt tgtaattctt ggcgtaagtt ccgatccaat aaatttacaa
      661 aacaaataaa taaaagaaaa taaattgctg ttatcatctc ttgatagtgc gtaaaattaa
      721 tattttcaac ttcttcgata aaatctatca ttggaatggt aataaagtac aatatcatgc
      781 taaagttggc aatagatata atctttctag tacgttgatt tagtttcttt tttcttaaat
      841 gactaaagtc ataattaaaa gctt
//
LOCUS       DAUELZ010000202          851 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.203, whole genome shotgun sequence.
ACCESSION   DAUELZ010000202 DAUELZ010000000
VERSION     DAUELZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.203"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            44..>851
                     /locus_tag="VHI09_001710"
     CDS             44..>851
                     /locus_tag="VHI09_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ6121538.1"
                     /translation="MESIENSYGRLLLESQLPDSARQLAQPLKSVVISRGKMICQRCH
                     YQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPPMHFPKGNYLVWSGQLTAYQEMI
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESFPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFHNRPLVIP"
BASE COUNT          281 a          151 c          173 g          246 t
ORIGIN      
        1 caagaaaaga taaaaattag caaaaaaaag cgaataataa agtatggagt ctattgaaaa
       61 tagttatggg cggttgttgc tagaaagcca gttaccagat tcagcaaggc agcttgctca
      121 acctttaaaa agtgtagtaa tatcgagagg aaaaatgatt tgtcaacgtt gtcactatca
      181 gcttgatgaa gaagcacgat tgccaagtgg agcctattat tgccgatttt gtcttgtctt
      241 tggacgaaat caatcagata aattgctcta tgctatccca cctatgcatt ttccaaaagg
      301 taattatctg gtttggagcg gtcaactaac tgcttatcaa gagatgattt cccaacaatt
      361 attaatcaat atgcaaaatc aaaaaacaac attagttcat gctgtaactg gtgcaggaaa
      421 aacagaaatg atttatgctg cgattgaggc ggtaattaac acaggtggct gggtttgcat
      481 tgccagccca agagtagatg tttgtgtaga ggtggctact cgtttatccc aagccttctc
      541 ctgcagcatc tgtttaatgc acgcagagtc ttttccttat caaagagctc ctattatagt
      601 agccacaacg catcagctgc taaaatttca caaggctttt gatctcttaa ttattgatga
      661 agtagacgca ttcccttttg taaataatat tcaacttcat tatgctgcaa gtcaggcttt
      721 aaaagaaggt ggcgcaaaga ttttattaac tgcaacttca actagaactt tagaaagaaa
      781 ggtaaacaag ggagaagtag tcaagctgac attggcgaga cgctttcata ataggccatt
      841 agttattccg a
//
LOCUS       DAUELZ010000203          846 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.204, whole genome shotgun sequence.
ACCESSION   DAUELZ010000203 DAUELZ010000000
VERSION     DAUELZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.204"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..439
                     /gene="entC3"
                     /locus_tag="VHI09_001711"
     CDS             <1..439
                     /gene="entC3"
                     /locus_tag="VHI09_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterotoxin type C3 EntC3"
                     /protein_id="HEQ6121539.1"
                     /translation="YYNCYYSEGNSCKNAKKTCMYGGVTEHHRNQIEGKFPNITVKVY
                     EDNENILSFDITTNKKQVTVQELDCKTRKILVSRKNLYEFNNSPYETGYIKFIESSGD
                     SFWYDMMPAPGAIFDQSKYLMLYNDNKTVSSSAIAIEVHLTKK"
     gene            552..734
                     /locus_tag="VHI09_001712"
     CDS             552..734
                     /locus_tag="VHI09_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEQ6121540.1"
                     /translation="MLTYNEFKQAIDNGYIAGDTVAIVRKDGQIFDYVLPHEKVKNGE
                     VVTKEKVEEVLVELSR"
BASE COUNT          324 a          108 c          168 g          246 t
ORIGIN      
        1 ctactataac tgctattatt cggaaggaaa tagttgtaaa aatgccaaaa aaacttgtat
       61 gtacggaggt gttactgagc accatagaaa tcaaattgaa ggtaagttcc cgaatattac
      121 agtaaaagtt tatgaagata atgaaaatat actttcgttt gatattacaa ctaataaaaa
      181 acaagtcact gttcaagaat tagattgtaa aaccagaaaa atattagtgt ctcgaaaaaa
      241 tttgtatgag ttcaataatt ctccgtacga gacaggctat attaaattta tagagagctc
      301 aggagacagt ttttggtacg atatgatgcc cgcacctgga gcaatatttg atcagtctaa
      361 atacttaatg ctatataacg ataataagac agtgagctct tcagctatag ctatagaggt
      421 tcaccttacc aaaaaataaa agaaaataac tttatgtatc aaatccatga tactagttaa
      481 attcaaaatg attgatacta aaaagttaat ttaagctatg agaatacgaa taaataagta
      541 ggagagtaaa aatgctaaca tacaacgagt ttaagcaagc gattgacaat ggatatatcg
      601 caggcgatac agtagcgatc gtgcgtaaag acggacagat ttttgattat gtgttgccac
      661 atgagaaagt aaagaatgga gaagttgtga ctaaggaaaa agtggaagag gtgctggtgg
      721 agctttcgag ataagtattt tctttgcccc ctgagaagta gttttattaa aggggtaaat
      781 agggggcata agtttaaaac ttacatagtt gattcgaagc aattatataa agtttcgtta
      841 ttttgt
//
LOCUS       DAUELZ010000204          843 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.205, whole genome shotgun sequence.
ACCESSION   DAUELZ010000204 DAUELZ010000000
VERSION     DAUELZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.205"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..>843)
                     /locus_tag="VHI09_001713"
     CDS             complement(<1..>843)
                     /locus_tag="VHI09_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ6121541.1"
                     /translation="VLEIAIQSIIMFLAVREAFLGILLVGNVTTFIRSLSTMQTSTQN
                     IVNNIYSFYNGSLYIELLYDFTHDELVHNPIIESTVDTIQSVEFERVSFSYNGKDNVI
                     NDISFSIKAGERLAIVGENGSGKSTIFKLACGLYDNYEGNIYINGINLRSIQKKSYYK
                     RISALFQDFLKYELTLRENVGLGELSKLYSDEDLISALKYTGVDSIFYFNQEKLNQID
                     LEQQLGNWFEDGRQLSGGQWQKIALSRVYLKEADCYLLDEPSSALDPESETKIFKTFF
                     ELSQ"
BASE COUNT          284 a          165 c           97 g          297 t
ORIGIN      
        1 tttgcgataa ttcaaaaaat gttttaaaaa tttttgtttc agactcagga tctaacgcag
       61 aactaggctc atctagcaaa tagcaatccg cttctttgag atatactctt gataatgcga
      121 ttttttgcca ctgtccacca gataactgtc tcccatcctc aaaccaattc cctaattgct
      181 gttccagatc tatctgattt aatttttcct gattaaagta aaatatcgaa tcaactcctg
      241 tatacttcag tgcacttatt aaatcctcat cactatataa ctttgataat tcacccaatc
      301 caacattttc acgcagtgtc aattcatact ttaaaaaatc ttggaacaat gcggatattc
      361 tcttgtagta tgactttttc tgtattgaac gaagattaat accatttata tatatattgc
      421 cttcataatt atcgtaaaga ccgcaggcta atttgaaaat cgtacttttt ccagaaccat
      481 tttcaccaac aattgctaac ctctctcctg ctttaattga aaaacttata tcgtttatta
      541 cattatcctt accattatat gaaaaagaaa ctctttcaaa ttcaactgac tgaattgtat
      601 caaccgtact ttcgattata gggttatgta ctaactcatc atgcgtaaaa tcatacaata
      661 actctatgta caatgaacca ttatagaaag aatagatatt atttactata ttttgtgtac
      721 ttgtttgcat tgtagataaa gaccttataa aagtagttac atttccaact aataatattc
      781 ctagaaatgc ttctctaact gcaaggaaca taataataga ttgtatagca atctccaata
      841 cac
//
LOCUS       DAUELZ010000205          834 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.206, whole genome shotgun sequence.
ACCESSION   DAUELZ010000205 DAUELZ010000000
VERSION     DAUELZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.206"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(65..>834)
                     /locus_tag="VHI09_001714"
     CDS             complement(65..>834)
                     /locus_tag="VHI09_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEQ6121542.1"
                     /translation="GITNYLLLEQEFFSVELNQIGNYLLKYIETILNKVTNCSEDREA
                     EFDLGIAHGITGPMLILAKLKSEKILSVEVGDILNKAINLMFLFRRDDKLWPGKIYTS
                     NILDLDLNYLPTRMAWCYGTPGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVY
                     SLTMCHGLAGVTFIYDLIGKNYSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGE
                     IEVELESIGILQGVSGIVLFLLNPYSDEELLWQRIFI"
BASE COUNT          279 a          162 c           98 g          295 t
ORIGIN      
        1 tttgcaatgg aatacgaatc ataccagttg gaaatacctt atacattttg ttctcctttt
       61 caaattaaat aaatattctt tgccacaata gctcctcgtc actgtaagga tttaataaga
      121 ataacactat ccctgatacc ccttgcaata tccctattga ctccagttca acttctattt
      181 ctccaaattt ttctctgtca gtatacccaa atacttttgt agttgaaaac gatgtaatta
      241 tttgttggcg aagctcttct gcaaagcgag ttagcctctc acttgaataa tttttaccaa
      301 tcaagtcata tataaaagtt actcctgcca aaccatggca cattgtaaga gaatagacat
      361 ttttgtctga acttctcatc tgagcaatta aactttcatt aatatcgtca ataaaattta
      421 aatctatcaa ttgacacatt ttaaaaaggg caactgcagt tccaggcgta ccgtaacacc
      481 atgccattct agtaggaaga taatttaaat caagatccaa aatattacta gtataaattt
      541 ttccaggcca tagtttgtca tcccttctaa ataaaaacat taaatttata gctttattca
      601 gtatatctcc tacttctaca ctcaaaattt tttctgattt taattttgct aggattagca
      661 taggaccagt aattccatgt gcaattccta gatcaaattc agcttctcta tcttcagaac
      721 aattagttac cttatttaaa attgtctcaa tatatttcaa aagataattt ccaatttgat
      781 ttaactctac agaaaaaaat tcttgttcaa ggagtaagta attagttatt ccac
//
LOCUS       DAUELZ010000206          823 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.207, whole genome shotgun sequence.
ACCESSION   DAUELZ010000206 DAUELZ010000000
VERSION     DAUELZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.207"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            250..>823
                     /locus_tag="VHI09_001715"
     CDS             250..>823
                     /locus_tag="VHI09_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ6121543.1"
                     /translation="MNLKVHSGEFVAIIGKSGSGKTTLAKMLLGYVSPSSGDITYNNI
                     SYSKIDKTDFRNISAFVSQDSPIFDGDVMYNISLGRESVSGEQVIETCKRVSLYEDIR
                     SMPMKFHTPLFRDNPSLSGGQKQRISLARELVTTPRILVLDEPTSALDVKTERIIQKN
                     VEALHCTRVLVTHRLNTVEKADKILIMDNGK"
BASE COUNT          291 a          109 c          168 g          255 t
ORIGIN      
        1 agtagcagtg gtgagcttat tcttctttat atgatggttt taaattttat taatcccatt
       61 aataatattt tgaattcgat tgatgagatt ctatatggtg ttaagcatta cgaaagggta
      121 ttagagatat caactcttga tgatgaggcg aatggcactt ataagctatc agaggataca
      181 gatatagaga taaatttaga aaatataaac tatcactatg agttaaatgg ggcagaggtg
      241 ttaaatggaa taaacctaaa agtccattca ggagaatttg ttgcaattat agggaaaagt
      301 ggaagtggaa agacaactct agctaagatg ttgttaggct atgtaagccc tagtagtgga
      361 gacatcacat acaacaatat atcctactct aaaattgata aaactgattt taggaatata
      421 tcggcttttg tatctcaaga ttctcccata tttgatgggg atgtgatgta taacatttcg
      481 ctagggagag aatctgtttc aggagaacag gttattgaaa cttgtaaaag ggtatcacta
      541 tatgaggata tcaggagtat gccaatgaag tttcatactc cactttttcg agataatcca
      601 tcactatctg gggggcaaaa acaacgaatt tctttagcaa gagagttagt aactacccct
      661 agaatcttag ttcttgatga acctacatca gctttagatg taaaaactga aagaataatc
      721 caaaaaaatg ttgaggcttt acattgtacg agggttttgg ttacccatag acttaataca
      781 gttgaaaaag ctgataagat tttaataatg gataatggca aaa
//
LOCUS       DAUELZ010000207          810 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.208, whole genome shotgun sequence.
ACCESSION   DAUELZ010000207 DAUELZ010000000
VERSION     DAUELZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.208"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            16..>810
                     /gene="sda3"
                     /locus_tag="VHI09_001716"
                     /gene_synonym="mf3"
     CDS             16..>810
                     /gene="sda3"
                     /locus_tag="VHI09_001716"
                     /gene_synonym="mf3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase Sda3"
                     /protein_id="HEQ6121544.1"
                     /translation="MSKSNRRTWQGLVVILIAILTTFTTSTVTAARKIRNFPDTTEIL
                     LGTKATETPGILPFTGSYQLVLGDLDNLQRPTFAHIQLKDQDEPNIKRKGLKFNPPGW
                     HNYKLTDANGKTTWLMDRGHLVGYQFSGLNDEPKNLVTMTKYLNTGFSDKNPLGMLYY
                     ENRLDSWLALHPNFWLDYKVTPVYHKNELVPRQVVLQYVGIDENGDLLQIKLGSEKES
                     VDNFGVTSVTLDNVSPLAELDYQTGMMLDSTQNEEDSNLETEEFEEA"
BASE COUNT          283 a          144 c          155 g          228 t
ORIGIN      
        1 aaggaggaca tggatatgtc taaatcaaat cgtcgtactt ggcaaggttt agttgttatt
       61 ttaatagcta ttctcaccac ttttaccaca agtactgtta cggcagccag aaaaattaga
      121 aatttccctg ataccacgga aattttgtta ggaacgaagg cgactgagac accaggaatc
      181 ttaccattca ctggtagcta ccaattagtt ttgggcgatc ttgacaatct gcaaaggcca
      241 accttcgcac acatccagct aaaagatcaa gatgagccta atattaaacg aaaaggactt
      301 aaattcaatc ctcctggctg gcataattac aaattgactg acgctaatgg aaaaacaact
      361 tggttaatgg accgtggcca tttagttggt taccaattta gcggcttaaa tgacgagcct
      421 aaaaacctag ttacaatgac aaaatatctt aatactggct ttagtgacaa aaatccttta
      481 ggaatgctct attatgaaaa tagattagat agctggttag ctctacaccc taacttctgg
      541 ctagactata aagttactcc tgtttatcat aaaaatgagt tagttcctcg ccaagtagtt
      601 ctacagtatg ttggaattga tgaaaatgga gatctacttc aaattaagtt aggtagtgaa
      661 aaagaaagtg tagacaactt tggagtaaca tcagttacat tagataacgt atctccttta
      721 gctgaattgg attaccaaac aggaatgatg ctagattcaa ctcaaaacga agaagatagt
      781 aatttagaaa ccgaagagtt tgaagaagcg
//
LOCUS       DAUELZ010000208          799 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.209, whole genome shotgun sequence.
ACCESSION   DAUELZ010000208 DAUELZ010000000
VERSION     DAUELZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.209"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            51..>799
                     /gene="mf2"
                     /locus_tag="VHI09_001717"
     CDS             51..>799
                     /gene="mf2"
                     /locus_tag="VHI09_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEQ6121545.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAARVRTYPNVSHANTH
                     YKNTVSSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVAMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINLDYLNGTATP"
BASE COUNT          265 a          148 c          145 g          241 t
ORIGIN      
        1 aatatttgat ccactctaaa agctaaaaat ggaaaggttt aggtgttatc atgaaattat
       61 ctaaacaaaa ggcaagtttg cttaccgctg ttttactgtt actttctctt tctataacga
      121 caataactgt tgacgcagct agggtacgaa cttatccgaa tgtatcccat gcaaacacac
      181 attataaaaa tactgtttct agtaagctct taccttttac tgctaattat cagttacaac
      241 ttggtgagtt ggataacctt aaccgcgcta ccttttcaca tatccaactg caagatagac
      301 acgaaactaa agatgtacgc actaaaataa attatgaccc tgtgggatgg cacaactatc
      361 aatttccata cggagatggc tcaaaatctt catgggtaat gaatcgtggg catctagtcg
      421 gatatcaatt ttgtggatta aacgatgaac caagaaattt agtggcaatg acagcctggc
      481 taaacacagg tgcttattca ggagcaaatg acagcaatcc tgaagggatg ttatactatg
      541 aaaaccgttt agattcttgg cttgcacttc accctgactt ttggctagat tataaagtca
      601 ctcctatata tagtggcaat gaagtagtgc ctcgacaaat tgaattacag tatgttggaa
      661 ttgattcatc tggtgagttg cttactataa ggctaaatag taataaagaa agtattgatg
      721 aaaatggtgt tactacagta atcttagaaa actctgctcc aaatatcaac cttgactatt
      781 taaatggaac tgccactcc
//
LOCUS       DAUELZ010000209          793 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.210, whole genome shotgun sequence.
ACCESSION   DAUELZ010000209 DAUELZ010000000
VERSION     DAUELZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.210"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            284..511
                     /locus_tag="VHI09_001718"
     CDS             284..511
                     /locus_tag="VHI09_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEQ6121546.1"
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAI
                     GFPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            524..721
                     /locus_tag="VHI09_001719"
     CDS             524..721
                     /locus_tag="VHI09_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEQ6121547.1"
                     /translation="MTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHPV
                     LAGGCVVGFTVGGAYLGYTAN"
BASE COUNT          255 a          103 c          182 g          253 t
ORIGIN      
        1 aactctacta tacaagattt attccttttt gtgtgtttac acaagatatt ttacactacc
       61 atcaaaaatc ttaaaaggta tttataaaag catgaatggt attatttaaa cttcttaatt
      121 aactattgtc acagcgtaag tgtagagagg ataaaaagac atttcatgat gaaaaaacgg
      181 ccttttaaga ttttgaagcc atgatactgt tctttatgat attctgtact agtcgaggtt
      241 gcaacgagac aaatttaaga attatgaaaa ggagttattc gatatggaga taaagaaact
      301 ggaaacattt catcaaatga ctatagaaaa gcttgccaag gttgagggtg gtaagaataa
      361 ttggcaggca aatgtcagtg gggttatcgc tgcaggtagt gctggagctg ctataggttt
      421 tccagtttgc ggtgtagcct gtggttatat tggagctaaa acagctatta ctctttgggc
      481 tggtgtaaca ggagctacag gcggcttcta gaggaaagaa aggatgacaa tgaaggaatt
      541 aacaattaat gacatggcta gtataagtgg aggaaacgct ccaggagatg ctgtaattgg
      601 tggtcttgga ggcttagctt ctggattgaa attttgtaaa ctgccacatc cagtattggc
      661 aggtggatgt gttgtaggat ttacagttgg tggagcatat ttgggatata cagcaaatta
      721 aatgatctat acgattttta ttattatgaa tgcgtctata atgtttttaa tcattgttga
      781 tgccattcat aaa
//
LOCUS       DAUELZ010000210          787 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.211, whole genome shotgun sequence.
ACCESSION   DAUELZ010000210 DAUELZ010000000
VERSION     DAUELZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.211"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            124..420
                     /locus_tag="VHI09_001720"
     CDS             124..420
                     /locus_tag="VHI09_001720"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ6121548.1"
                     /translation="MNSHHACQTLQVSRSGFYAYLKRRPSSRHVENEALKEMIKAIFY
                     EHKERYGSVRITQELCRRGIHVNHKRVGRLLHQLGLYAKGSRYQYKYYNRRRSS"
     gene            427..787
                     /locus_tag="VHI09_001721"
                     /pseudo
     CDS             427..787
                     /locus_tag="VHI09_001721"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          267 a          143 c          157 g          220 t
ORIGIN      
        1 aaaaaaactt gaaaaagagc acaaactgct acaggaggaa ttagctcttc taaaaaagtt
       61 ccaggtcttc ttgaagccaa actggaaata aaatttcagt ttctgaagga aaatagtgtt
      121 agcttgaata gtcatcacgc ctgtcaaact cttcaagtct cccgttcagg tttctatgct
      181 tatttaaaac gtcgtccctc ttcaaggcat gttgagaatg aagctttgaa ggaaatgatt
      241 aaagccattt tctatgaaca taaagagcgt tacggaagtg tacgaattac ccaagaactt
      301 tgtagacgtg gcatccatgt caatcataaa cgtgttggta gactccttca tcagctaggt
      361 ctctatgcta aaggaagtcg atatcaatat aaatactata accgtcgacg ttcttcatga
      421 actcgtccca atcttgttaa tcaatgtttc caagcgactg gtaagaataa attatggcta
      481 ggagacttaa cctatattcc tacacaagaa ggaatacttt atgtgtctgt ctttatagac
      541 gtttatagta gaaagattgt tggttgggca atgggccgac gtatgcaaga caagttggta
      601 acagaggctt tcaatcaagc ttataataga gaaaagccta aagaaggggt aatcgtccat
      661 acagatcagg gttctcaata taccggagca cgatttcagg acctacttag acggaaaaaa
      721 tgtaagtcca gtatgagtcg aaaaggtaat ccatacgata atgcactcat ggaatccttt
      781 tataaaa
//
LOCUS       DAUELZ010000211          779 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.212, whole genome shotgun sequence.
ACCESSION   DAUELZ010000211 DAUELZ010000000
VERSION     DAUELZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.212"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            201..779
                     /locus_tag="VHI09_001722"
                     /pseudo
     CDS             201..779
                     /locus_tag="VHI09_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
BASE COUNT          290 a          116 c          162 g          211 t
ORIGIN      
        1 attatatgat gtcaattttt tcaagattac tttaaaaata aggaagtcgg tgtttcagat
       61 cgattagaaa gtctacagaa acagtacatg cttttcaaga agaatgagat aaagaggagg
      121 tatccactga agagagtaag gaagtcttca agaaatacaa ggggcaacga gatgccatcc
      181 atgagtttga agttgaactt gtggaacatc aaattgagag gttaattgct gaggacattt
      241 atatcaaggt gtcttttggt gtaaagcaga gtggtcttat tttcattcct aactatcaat
      301 tggatattag gaaagaagaa aatcataaaa aatataaagt ctatattcgt gagaccgcac
      361 aattcttcat ttacaacaag gaggcgtcta aacttaatcg ctatgtgcga gggcgtgagc
      421 tcatttacca gttaaccaat gatagcaaga gtattccaaa aagaagacga ccaaaaccat
      481 tgatacactt aagaaaaaga ttgaagaaat aagtctcctc attgagttag atacggagaa
      541 taagccttat caagatatta aagatgacat tgttaaagat atggctcaat tagatttgac
      601 cattacagag ttgcaggacc atattgctca tctcaataag gttgcggagg tcttgcttaa
      661 tctgaacaac aacgatatag agaaccgccg tttggccaga tatgactatg ccaagatgaa
      721 cttgactgca gctataaaaa tagaagaagt tgagaaagag attgaaactt ctcaaaatg
//
LOCUS       DAUELZ010000212          776 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.213, whole genome shotgun sequence.
ACCESSION   DAUELZ010000212 DAUELZ010000000
VERSION     DAUELZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.213"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..>776)
                     /locus_tag="VHI09_001723"
     CDS             complement(<1..>776)
                     /locus_tag="VHI09_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ6121549.1"
                     /translation="SIVIPDLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQYIGG
                     DGKLTETIANYSQQLLKQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLC
                     RQGNTLVDTYTGKYAKRIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPF
                     AGTIGVLVAFTALIPVIGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYP
                     KVVGGSIGLPAMWVLMAITIGASLKGIVGMIIAVPLAATL"
BASE COUNT          297 a          146 c          114 g          219 t
ORIGIN      
        1 taaagtgtcg ctgctaaagg aactgcaata atcataccaa ctattccctt taaagaagcg
       61 cctattgtaa ttgccattaa tacccacata gccggtaatc caatcgatcc accaactact
      121 ttcggataaa taaaattacc ttcaatctgc tgcaaaataa ttaaaaaaat gataaaaata
      181 atagcctgtg acatggactg agtcataatt aaaataaatc caatagctgc tccaattgaa
      241 gcacctataa ctggaatcaa tgctgtaaaa gctactaaaa cgccaatagt ccctgcaaat
      301 ggtaatctta atataaacat gccactagca gtcagcgaac ctaaaatcat agcttctaag
      361 gtctgactaa caaaaaatcc gtgaaaacgc tgatgcaaca actctaacaa gtaatgaatg
      421 cgtttagcgt acttaccagt ataagtatct actaaagtat ttccctgacg acaaagatct
      481 tctttacttg ctaaaacgta taacgaaaaa acaaaactaa taaaaagatt aataattgca
      541 gaagcaataa ccgtgacaga tgttaaaata tttgtcaaaa cagtaagaaa ttgctttaat
      601 aactgttgac tatagttagc aatcgtctct gtaagtttgc catcgccacc aatatactga
      661 atagtacgtg ctaacagttt attgtgctca agattgttaa caacttcttg tatcgttata
      721 gtatcaaatt ttgttagagt actaatactt gtaattaagt ctggaatgac aatgga
//
LOCUS       DAUELZ010000213          767 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.214, whole genome shotgun sequence.
ACCESSION   DAUELZ010000213 DAUELZ010000000
VERSION     DAUELZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.214"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..345)
                     /gene="csn2"
                     /locus_tag="VHI09_001724"
     CDS             complement(<1..345)
                     /gene="csn2"
                     /locus_tag="VHI09_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEQ6121550.1"
                     /translation="MNLNFPLLDEPIPLRGGTILVLEDVCVFSKIVQYCYQYEEDSEL
                     KFFDHKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVA
                     TITELIVFECLEN"
     gene            complement(335..676)
                     /gene="cas2"
                     /locus_tag="VHI09_001725"
     CDS             complement(335..676)
                     /gene="cas2"
                     /locus_tag="VHI09_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ6121551.1"
                     /translation="MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSI
                     YSKLLLNNTANNAMIGRLREHNPNKGNITLLTVTEKQFARMIYLHGERNNCIANSDER
                     LVFLGEAFDES"
     gene            complement(673..>767)
                     /locus_tag="VHI09_001726"
     CDS             complement(673..>767)
                     /locus_tag="VHI09_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="subtype II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ6121552.1"
                     /translation="MYLTNIISDYTKKVVKALNNEGKGVPEFRI"
BASE COUNT          248 a          146 c          105 g          268 t
ORIGIN      
        1 attttctaag cattcaaaga caatcagttc tgtaatcgta gcaaccaatt tgtcaatcat
       61 cgatttcact tcgggtttct cattaaattg agattctaaa tctgcatgaa tcaattttaa
      121 aatggttgag gagttaacat caaatcctaa aatatctgtt acaagcatga tttctgattc
      181 tttgatggtt ttcatcttgt gatcaaaaaa tttaagttca gaatcttcct catattggta
      241 acaatattgc actatttttg aaaatacaca gacatcttcg agcacaagaa ttgtaccgcc
      301 tcttaatgga atcggttcat ctagtaaggg aaaattaaga ttcatcaaaa gcctccccaa
      361 gaaatacaag tctttcatcg gagtttgcaa tacaattatt tctttcacca tgtaaataaa
      421 tcattcgtgc aaactgtttt tccgtgactg ttaataaagt aatatttcct ttattaggat
      481 tatgctccct cagccgacca atcatggcat tgttagctgt attattcaat agcaacttac
      541 tataaataga aaattgatgc atgataaacc cttcactaag taaaaatttc cgaaattttc
      601 gataggcttt tcgttcctca gcggtgtccg tcggcatatc aaacataagt atcattctca
      661 tatatctata actcatatcc taaattcagg aactcctttc ccttcattat tcagagcttt
      721 gacaactttt ttagtataat cgctaataat attcgtgaga tacatct
//
LOCUS       DAUELZ010000214          751 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.215, whole genome shotgun sequence.
ACCESSION   DAUELZ010000214 DAUELZ010000000
VERSION     DAUELZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.215"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..645)
                     /locus_tag="VHI09_001727"
     CDS             complement(<1..645)
                     /locus_tag="VHI09_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121553.1"
                     /translation="MTLIDTAFIWLATILRQKQGDIFKRIISIISLTIWQYLVSVLTR
                     GTPLFLSSGLQIILLYCYTVEITNLILYGHKQFKDKLDKGLLIIIFVSITSLFVNRIL
                     FNFLFLMVFTILHLYPLLVIVLYYRSFRQQISVVRRSLILFSLLLLVILGSELYGEML
                     DVNQAFNNLGWYLFPLIMSVIYYFKTIHDKLSFVIQRWLGDYKARIELLFLLLIL"
BASE COUNT          303 a          117 c          110 g          221 t
ORIGIN      
        1 aaggataaga agtagaaata atagttctat acgagctttg taatcaccta accatctttg
       61 gataacaaaa ctcaatttat catgtatggt cttaaaatag tatattacac tcattataag
      121 gggaaacaga taccatccca aattattaaa ggcttgatta acatctagca tttctccata
      181 aagttcacta ccaagtatta ccagcaaaag taaagaaaat agtatcaatg aacgtctaac
      241 aactgatatt tgttgcctaa aggacctata atatagcact ataactagta acggatacag
      301 gtgtaaaata gtaaaaacca tcaggaataa aaaattaaat aaaattctat taacaaaaag
      361 gctagttata gaaacaaaaa taattattaa caagccctta tctaacttat ctttaaattg
      421 tttgtgccca tataatatta aattagtaat ctcaacggta taacaataga gcaatataat
      481 ctgtagtcca ctgcttaaaa agagaggagt tcctcttgtc agcacagata ctaagtattg
      541 ccaaattgtt aaggagatga tagaaataat tcttttaaaa atatcacctt gtttttggcg
      601 taatattgtt gccagccaaa taaaagctgt atcaataagg gtcattacaa gagctacttt
      661 ttcagagaaa agaagagaca ggttctctcg attatagacc aaaattaaca aggctattaa
      721 ttgttgcaac aagattattg cagtcccaaa g
//
LOCUS       DAUELZ010000215          719 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.216, whole genome shotgun sequence.
ACCESSION   DAUELZ010000215 DAUELZ010000000
VERSION     DAUELZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.216"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            399..>719
                     /locus_tag="VHI09_001728"
     CDS             399..>719
                     /locus_tag="VHI09_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HEQ6121554.1"
                     /translation="MKSYLCSNFLKIKNTSYLLIHILAALLFPLLLFFYWSQRGGLQS
                     RTVLFSYFQIVGVLLPFVASIVCIQLKNLEESSGKYKYLLGYSQSNYKPFIVELVFLW
                     LCYCI"
BASE COUNT          217 a          106 c          105 g          291 t
ORIGIN      
        1 gtaattagtt ttgtcctacc tactccaagt cttattagta taggtaggat actattaact
       61 atcttgttaa tttggttgac tacactatgg caaatcccgt ttattttatg gctatcaaga
      121 aaaattaatg tgtattttgc tatgattatt aatattatat ctccactaat tattggtaca
      181 agtttttctc tcttaaataa atggtatttg ttcccttatg attggtcgtt gaagttgctt
      241 gagccaatga caagaatgag aataaatagt ataccttttg gagcggagtt tgttccagac
      301 tactcacaga tttttatatc attgttccta ggaattgctt ttttcatctt actgaccaat
      361 ctatttgcta tctctttcaa aaaacaggtg aaatagtcat gaaatcatat ttatgttcta
      421 attttttaaa aatcaaaaat acttcatatt tactaataca tattttagct gcactattat
      481 ttcccttact tctatttttc tattggagtc aacgtggagg tttacaaagc aggactgtat
      541 tattctccta ctttcaaata gtaggtgtac ttcttccttt tgttgcaagt attgtgtgca
      601 ttcaactaaa aaatttagaa gaatcatctg gaaaatataa atatttattg ggttactcac
      661 agtcgaatta taagccattt attgtagaat tagtatttct atggttatgc tattgtata
//
LOCUS       DAUELZ010000216          715 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.217, whole genome shotgun sequence.
ACCESSION   DAUELZ010000216 DAUELZ010000000
VERSION     DAUELZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.217"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            326..610
                     /locus_tag="VHI09_001729"
     CDS             326..610
                     /locus_tag="VHI09_001729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121555.1"
                     /translation="MQGEYDEEAELSSKDREFGRQNGQKDGKEGKNGFGPTINRDDIQ
                     VPEKVKHQDEYQDGYEQGYREGWQQEHPLLSFLKHILGSMFFLDYHCNKY"
     gene            complement(546..715)
                     /locus_tag="VHI09_001730"
                     /pseudo
     CDS             complement(546..715)
                     /locus_tag="VHI09_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429453.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
BASE COUNT          266 a          111 c          134 g          204 t
ORIGIN      
        1 aaagctgatt aacagcctaa aacaaactct ttctctgaca agaaaaagcg ttatgctttc
       61 cgaaccattt tgtcttttaa ataataaaaa atgagcttga aatgaacttg aaatgaaaca
      121 gtgagaggta agagatataa aataagatcg tcgaaaataa agaaacggag taatttttat
      181 gagaacaaaa tccaactact ttttaaactt agcaactctt tgcctagctc tttttggagc
      241 aattttacta atggcacaac cagcaaatgc tgaggaagta tctccaaaca ttggagttga
      301 gggtacttct tagtattatg gtgaaatgca gggagaatat gatgaggaag cagagctctc
      361 ttcaaaagat agagaattcg ggagacaaaa tggtcaaaaa gatggcaaag agggtaaaaa
      421 tggatttggc ccaacaataa atcgagatga tatacaagtt cctgaaaaag taaaacatca
      481 agatgaatat caggatgggt atgaacaagg ttatcgtgag ggatggcagc aggagcatcc
      541 acttctatct tttttaaaac atattctagg ctcaatgttc tttctggatt atcactgtaa
      601 taaatactaa accctcggtt aagcatctca gcttttagca ttagttccca agcattacat
      661 ataaaaaaac taaagccttc aattcgatat tttattgtcg gcttattata tattt
//
LOCUS       DAUELZ010000217          693 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.218, whole genome shotgun sequence.
ACCESSION   DAUELZ010000217 DAUELZ010000000
VERSION     DAUELZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.218"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..332
                     /locus_tag="VHI09_001731"
     CDS             <1..332
                     /locus_tag="VHI09_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEQ6121556.1"
                     /translation="ENLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNG
                     ERFSDSTLKKGDKLFYTEDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPVRGGASAI
                     ISIKKKI"
     gene            489..629
                     /locus_tag="VHI09_001732"
     CDS             489..629
                     /locus_tag="VHI09_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HEQ6121557.1"
                     /translation="MTNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTT
                     VK"
BASE COUNT          256 a           89 c          129 g          219 t
ORIGIN      
        1 aggaaaatct gataagagct ctttctaacc ttttagttaa tgcaataaga tttatgcccg
       61 tattagataa aaaaatcgaa gttatactgt ctgagtcagg agagcaaatt cattttgaaa
      121 tatggaataa tggtgagcgt ttcagtgata gtacactgaa aaagggagat aaattatttt
      181 acacggaaga ttatagcaga ggtaacaaac attatggaat cggtttggca tttgttaaag
      241 gcgttgctat caaacacggt gggaatcttc aattgaataa tccagttaga ggaggtgcta
      301 gcgccataat ctcgataaaa aagaaaattt aatagatttg attgaaactg gagtatttca
      361 ggcaaaaaat cgtaaatttt ggtgattttt ggctaaaata ctttttttct gaacaaattc
      421 tgaacttggg gtgtcataat aatcatgtaa atatatcgga tgcaaaaacg taagagagga
      481 actagaaaat gactaacaca attaaagact ttgatctcga tttgaaaaca aataaaaaag
      541 acactgctac accttatgtt ggtagccgtt acctatgtac ccctggttct tgttggaaat
      601 tagtttgctt tacaacaact gttaaataat aaagttatca ttatcatcaa cattataatt
      661 tcaaataatt tgatatattt ataaatttag ggg
//
LOCUS       DAUELZ010000218          681 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.219, whole genome shotgun sequence.
ACCESSION   DAUELZ010000218 DAUELZ010000000
VERSION     DAUELZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.219"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..233
                     /locus_tag="VHI09_001733"
     CDS             <1..233
                     /locus_tag="VHI09_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ6121558.1"
                     /translation="FKIRKFLMESKEIHLTKSPYIRGSLEIHSKNRKDEKINLYDAKP
                     NSTRSDVLKKYKDNKTINMKDFSHFDIYLWTK"
     gene            437..681
                     /locus_tag="VHI09_001734"
                     /pseudo
     CDS             437..681
                     /locus_tag="VHI09_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          276 a          102 c          105 g          198 t
ORIGIN      
        1 attttaaaat aagaaaattt ttgatggaat caaaagaaat tcatttgact aagtctcctt
       61 atataagggg gagtttagaa attcacagta aaaataggaa agatgaaaaa atcaatttat
      121 atgacgcaaa acccaatagc accagatctg atgtcttaaa aaaatataaa gacaataaga
      181 ctataaacat gaaagatttc agccattttg atatttatct ttggactaaa taagctaaaa
      241 gctttggata aatatgatca attgacctat aataacattg ataccaaatc taaaactgct
      301 cagattgaat tgcgccccaa aagttagaca aaaaatctaa taattggggt gtttttgtct
      361 ggcattaagt ttagctaata accttgctta aatcacatta aaccaataat ggaaaaagac
      421 atctccaaat aaatgctatg atggaactat atgatagagt gatcaaggtt ggcaatatca
      481 acacaactat tatcatcatt tcctaaactc taaaggaatt cgtccgtcta tgtcacgtaa
      541 aagaaacagt cctgacaatg gcatgatgga atctttcttt ggcatcttaa agtcagaaat
      601 gttctatggt tatgagaata actataaatc actagaagac ttagaacaag cgattacaaa
      661 cggcattgat tattacaacg a
//
LOCUS       DAUELZ010000219          671 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.220, whole genome shotgun sequence.
ACCESSION   DAUELZ010000219 DAUELZ010000000
VERSION     DAUELZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.220"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            100..372
                     /gene="rpsP"
                     /locus_tag="VHI09_001735"
     CDS             100..372
                     /gene="rpsP"
                     /locus_tag="VHI09_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEQ6121559.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            382..621
                     /locus_tag="VHI09_001736"
     CDS             382..621
                     /locus_tag="VHI09_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEQ6121560.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
BASE COUNT          223 a          118 c          120 g          210 t
ORIGIN      
        1 acttgacact ttctttaaac tgagttatac tgtttaacat aggaatttag cgtagctgta
       61 tttcttatac attactaaaa gaaaagagac tatttaaaaa tggcagtaaa aatccgttta
      121 actcgtatgg gttctaagaa aaaacctttc taccgtatta atgttgcaga ttcacgtgca
      181 ccacgtgatg gtcgttttat cgaaacagtt ggaacataca acccattagt ggctgaaaat
      241 caaattacta ttaaagagga tcgtgttctt gagtggttat ctaaaggagc gcagccttct
      301 gataccgttc gtaacatctt gtctaaagct ggtgttatgg ctaagttcca cgaccaaaaa
      361 ttctctaaat aataacagcc tatggatacc attgaaaatc ttattatcgc tattgtgaaa
      421 cctttgattt cacaaccaga taatcttacc attaaaattg aagatactcc tgacttttta
      481 gagtatcatc ttgacttgga tgctcaagac atcggtcgtg ttatcggcaa aaaaggtcga
      541 actattactg cgataagatc gattgtctac tcggttccaa cactaggaaa aaaagttcgc
      601 ttagttattg atgagaaata atcactaact tgagatggat tgaaaagctt gttgacttaa
      661 agtcaacaag c
//
LOCUS       DAUELZ010000220          669 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.221, whole genome shotgun sequence.
ACCESSION   DAUELZ010000220 DAUELZ010000000
VERSION     DAUELZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.221"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            45..>669
                     /locus_tag="VHI09_001737"
     CDS             45..>669
                     /locus_tag="VHI09_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEQ6121561.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEPIKIIFLGRASKKCFNSPIPLF
                     KIQKKAATKYLNRILPNLDVENYVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKAN
                     DTATMISYWPLTISEELALMIEGYFYKLDKNELPTPRFTKMGGDIKVM"
BASE COUNT          241 a           86 c          122 g          220 t
ORIGIN      
        1 acttatattc agaatgtgaa aaaatttatt tatgaggtgt aactatggat ttaaaaatta
       61 caaacggctt ttatgaccca agtcatttat catacgaagt agtagaacgc aaaggcttag
      121 gtcaccctga taccttagca gatggaattg ctgagcaaat tgaaattgat tactcacttt
      181 attgtttgga caagtttggt gttattccac atcataattt tgataagatt attatccgag
      241 ggggacattc agttcaggat tttggtggaa gtgactttat tgaaccaatt aaaattattt
      301 ttctaggtag ggcgagcaaa aaatgtttta atagtccaat tccacttttt aaaattcaga
      361 aaaaggctgc aactaaatat ttaaatagaa ttttacctaa tttagatgtt gaaaattatg
      421 tagaatttga gacattgacc tcagatttta ctacaaaaac taattggttc tcgcctgaag
      481 caatagaaga tttaccggag tatctagatg taccaaaggc aaatgataca gctacaatga
      541 taagttattg gccattaaca atttctgaag aattagcatt aatgattgaa gggtattttt
      601 ataaattaga taagaatgaa ttaccaacgc caagatttac taaaatgggt ggtgatatta
      661 aagtaatgg
//
LOCUS       DAUELZ010000221          662 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.222, whole genome shotgun sequence.
ACCESSION   DAUELZ010000221 DAUELZ010000000
VERSION     DAUELZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.222"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..503)
                     /locus_tag="VHI09_001738"
     CDS             complement(<1..503)
                     /locus_tag="VHI09_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ6121562.1"
                     /translation="MEIRNRDPHAVIVFVTTHSEFMPVSFQYQVSALDFIDKELPEEL
                     FSHRIEKAITYVQDNQGKTLAEDSFVFINVKSQIQVPFSDLLYIETSLIPHKLILYST
                     KQRVEFYGQLSEIVEQDDRLFQCHRSFVVNPYNISSIDRSERLVYLKGGLSCIVSRLK
                     IRSLIKVV"
BASE COUNT          217 a          124 c           98 g          223 t
ORIGIN      
        1 actactttta ttaatgatcg tattttcaat ctcgaaacaa tacaagataa tcctcccttt
       61 aaataaacta accgttcgga tctatctatt gatgatatat tatagggatt aacaacaaag
      121 gaacgatgac attgaaataa tcgatcatct tgctcaacaa tttctgataa ttggccataa
      181 aattctactc tttgtttagt agaatagaga atgagtttat ggggaattag tgaggtttca
      241 atatacagca aatctgaaaa ggggacttgt atctgggact ttacattaat aaaaacaaat
      301 gaatcctccg ctaacgtttt tccttggtta tcttgtacat aagttatagc tttttcaatg
      361 cgatgactaa ataactcctc tggcaactct ttgtcaataa agtctaaagc tgaaacctgg
      421 tattgaaaag ataccggcat aaattctgaa tgtgtggtca caaaaactat cactgcgtgc
      481 ggatctctat ttctaatctc cacagcaatt tccattcctt ttttatcttc tcccttaatg
      541 tcaatgtcaa gaaaaaaagc tgatgtcttc ctctctctga tatatcttca agcagctgat
      601 tgggtttacc ataaacttcc aagtggttaa cctccaactt attatcagtc aaaatcttat
      661 aa
//
LOCUS       DAUELZ010000222          644 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.223, whole genome shotgun sequence.
ACCESSION   DAUELZ010000222 DAUELZ010000000
VERSION     DAUELZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.223"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..590)
                     /locus_tag="VHI09_001739"
     CDS             complement(<1..590)
                     /locus_tag="VHI09_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ6121563.1"
                     /translation="MTIIILQIMRFGVYSSVDSSLVSVSNNASSYANRTMARISSFYF
                     DTENNIIKALPDSDSSKLLGTPAANTDIILFSANGTILNAFDAFSNYQNFHLDKRRLG
                     SIETTSLMNFYGQEEKYHTITVRVHIKNYPAVAYMMAVVNVEQLDRANERYERIIIIV
                     MSVFWLISILASIYLAKWSRKPILESYEKQKMFVENA"
BASE COUNT          237 a          117 c           88 g          202 t
ORIGIN      
        1 gcattttcaa caaacatttt ttgtttttca tagctttcta aaataggttt tctgctccac
       61 ttggctaaat aaatacttgc taaaatagaa attagccaaa aaacactcat aactataata
      121 ataatgcgct cataacgttc attagcgcgg tctaattgtt ccacatttac tactgccatc
      181 atataggcaa ctgcaggata atttttgata tgaaccctta cagttatcgt atggtatttt
      241 tcttcttgtc cataaaaatt cattaaactg gtggtttcaa tactccccaa ccggcgttta
      301 tctaaatgaa aattttgata gttagaaaac gcatcaaaag catttaaaat tgttccatta
      361 gcactaaaca aaatgatatc tgtattagct gcaggcgttc ctaataactt agaactatct
      421 gaatcaggca gcgccttaat aatgttattt tcagtatcaa agtaaaaaga agatattcta
      481 gccatcgtac gattagcata gctacttgca ttattactaa cagaaactaa actactgtca
      541 actgacgaat aaacgccaaa ccgcataatc tgtaagataa taatagtcat aatgacaaaa
      601 atacctgtaa aaaccgcaaa aaaatgaaaa aagtggttat agtt
//
LOCUS       DAUELZ010000223          644 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.224, whole genome shotgun sequence.
ACCESSION   DAUELZ010000223 DAUELZ010000000
VERSION     DAUELZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.224"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..>644)
                     /locus_tag="VHI09_001740"
     CDS             complement(<1..>644)
                     /locus_tag="VHI09_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ6121564.1"
                     /translation="LAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFKGYDL
                     ILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKPFSLK
                     QFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYREYEI
                     LELLSRHLYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKT"
BASE COUNT          211 a          124 c           77 g          232 t
ORIGIN      
        1 tagtttttat tggattaatt ccaaaacttg aaaatttcat tctaatctga tagatatatt
       61 ctgaaataga atgaaacaat gctgaagctt catcgctata tacttgttca tatatctctt
      121 cttttgtgaa aactttatac agatgacgtg acagtaattc taaaatttcg tactctctat
      181 aagtcaggaa taacggctgt ttatcaatat ataacatacg ttcctctaga tagatttcta
      241 taggtggaaa actgcgcttc actctctcct cagcctcatt ctttatcttt gctctctctt
      301 ctctcttcaa atgagagtta actttagcta cgaattgttt taagctaaat ggtttcacaa
      361 taaaatcgtc tccccctata tttaaagctt ggacaatttc attatcgcca tctaaagcgc
      421 taacaaaaat aattggagaa tgaacttctt cccgaatttt ataacaaaac tcagttccac
      481 ttatattagg catcatgata tccaataaga tcaaatcata tcctttaaaa tcatcaaaaa
      541 ctattggtaa ttctatctct tgacaggtta taacatgata attttcaatc tccaaagcag
      601 tcttcataag cttcagaatt tctttatcat catctatggc taaa
//
LOCUS       DAUELZ010000224          632 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.225, whole genome shotgun sequence.
ACCESSION   DAUELZ010000224 DAUELZ010000000
VERSION     DAUELZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.225"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..>632)
                     /locus_tag="VHI09_001741"
     CDS             complement(<1..>632)
                     /locus_tag="VHI09_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ6121565.1"
                     /translation="LVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGIYDLIL
                     LDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKPFYLEE
                     LKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVYLLQNQ
                     NVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRS"
BASE COUNT          210 a          138 c           69 g          215 t
ORIGIN      
        1 gcttcttaaa gtttgaagcc tgttaacaaa acgggtacct tttaattttt tccttatttt
       61 agaaatgtaa acttcaacaa ccgaaatcgt agtatcacta tcgaatcccc ataaacgatc
      121 aaaaatttgt gtctttggta aaataacgtt ttgattttgc aaaagataca ccaacaaatc
      181 aaattctttg ccaagtaatt caactacttt cccttcaacc ttcacctctt ttcgagctaa
      241 gtcgaccact aaattgccaa aactaatatt tttatctgca aatttccctg ttctttttaa
      301 cagtgcttga attcgcattt ttaactcttc taaatagaac ggctttgtca gataatcatc
      361 agcacctaat tcaaatccgt gacctttatc atctaaaccc tctttagctg tcatgatcaa
      421 tacaggaatt ttaatatctt tttctcgcaa ttcttttaag acttgaaagc catttttctc
      481 cggcaacata aggtctaaca aaatcaaatc ataaatacca ctttctgctt cgtataaacc
      541 ttcatcgcca tcaaaaactt gcatgacgtc tgcaaaatca tctaaaaaat caaaaatgga
      601 atttgataag ctcaagtcat cttctactaa ta
//
LOCUS       DAUELZ010000225          631 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.226, whole genome shotgun sequence.
ACCESSION   DAUELZ010000225 DAUELZ010000000
VERSION     DAUELZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.226"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            56..>631
                     /locus_tag="VHI09_001742"
     CDS             56..>631
                     /locus_tag="VHI09_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ6121566.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAE"
BASE COUNT          172 a           96 c          133 g          230 t
ORIGIN      
        1 taacatttat gtttttttaa aaataaacgt tcgtataata aaaaaggagt ttttcatgga
       61 aaaaacaaaa cgttatatca ttgcaacagc agggatactt ctccacttaa tgttgggatc
      121 gacctatgct tggagtgttt atcgtaatcc aattttacaa gagaccggtt gggaccaagc
      181 tcctgtcgct tttgcctttt ctttagctat tttttgttta ggtttatctg ctgcttttat
      241 gggaaattta gttgaacaat atggtcctcg tctaaccgga acggtgtcag ctattcttta
      301 tgcttctgga aatatgctga ctggtttagc gattgatcgt aaagaaattt ggcttttata
      361 tattggttat ggtgttattg gtggcttagg acttggagcg ggatatatca caccgatatc
      421 aactattata aaatggtttc cagataaaag aggcatggca acagggtttg ctattatggg
      481 gtttggtttt gcgtctttat taacaagtcc gattgcccag tggctaattg aaacagaagg
      541 tttggtagca acattttacc ttttagggtt gatttatcta atagttatgc tttttgcttc
      601 tcaattgata ataaaaccaa ctgctgctga g
//
LOCUS       DAUELZ010000226          630 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.227, whole genome shotgun sequence.
ACCESSION   DAUELZ010000226 DAUELZ010000000
VERSION     DAUELZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.227"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            44..>630
                     /locus_tag="VHI09_001743"
     CDS             44..>630
                     /locus_tag="VHI09_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ6121567.1"
                     /translation="MKKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIFGNVEILRK"
BASE COUNT          231 a           87 c          126 g          186 t
ORIGIN      
        1 ttaagaaaca agcgtcttgt aaaccatttc aaagtatgat agaatgaaaa agtaccttaa
       61 aaagatagga aaaatgatga caaagatgta ttatgatgaa aatccagata gcctacacga
      121 tattcatgaa gtaaaagtag agctgcttaa tcatcccttt acttttttaa cggattcagg
      181 tgtcttttca aagaaaatgg ttgatttcgg tagtcaagta ttattagaga cactaaattt
      241 caaggagaac gaacgtgtac tggacttagg ttgcggctat ggtcctcttg ggattagtct
      301 agccaaggtt caaagggtag acgcaacctt agtggacatc aataatcgtg ctcttgattt
      361 agctagaaaa aatgcaacta ataaccaagt ggcagtaact atttttcaat caaatattta
      421 tgaaaatatt agtggccatt ttgagcatat tattagtaat cctcctatta gagcaggaaa
      481 gagagtagtt catagcatta ttgaaaaaag catagatttt ttagtggtta atggtgactt
      541 aacgattgtg attcaaaaaa aacaaggagc tcctagcgct aaagcaaaaa tggcaactat
      601 ttttggtaat gttgagatac taaggaaaga
//
LOCUS       DAUELZ010000227          627 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.228, whole genome shotgun sequence.
ACCESSION   DAUELZ010000227 DAUELZ010000000
VERSION     DAUELZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.228"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..463)
                     /locus_tag="VHI09_001744"
     CDS             complement(<1..463)
                     /locus_tag="VHI09_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEQ6121568.1"
                     /translation="MINPSKQNQTIFLWNMLGSLSTAVISVILLMVVTRLLTSSDSDI
                     YAFAYSFANMMVVVGLFQVRNYQATDINEKYSFSQYLVARLMTCLLMLAITVIYLTLT
                     KTDSYKSTIVFLVCFYRSTDAFSDLYQGMFQQHERLDIAGKSLAYRNTLIFM"
     gene            complement(456..>627)
                     /locus_tag="VHI09_001745"
     CDS             complement(456..>627)
                     /locus_tag="VHI09_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEQ6121569.1"
                     /translation="SHFLGIHSPTNMLFFLGFCLSLAIIFSLTNNISLQNDKVKRLTQ
                     EVALMKKEKTND"
BASE COUNT          229 a          103 c           99 g          196 t
ORIGIN      
        1 ccataaaaat aagagtattt ctgtaggcta gcgatttacc tgcaatatcc agccgttcat
       61 gttgttggaa cattccctga tataaatccg aaaaagcatc tgtcgatcta tagaaacaga
      121 ctagaaagac aattgtactc ttgtagctat ctgtcttagt taacgttaaa taaattactg
      181 ttatggctaa cattaataaa caagtcatca gtcttgccac taagtactga ctaaaggaat
      241 atttttcatt aatatctgtt gcttgataat tacgaacctg aaaaagtcca acaactacca
      301 tcatattagc aaaagaatag gcaaaggcgt aaatatctga atcagacgaa gttagtaacc
      361 tagtaaccac cataagtaat ataactgata ttacagctgt tgataaactt cctaacatat
      421 tccataaaaa aatcgtttgg ttttgttttg aaggattaat cattggtttt ctcttttttc
      481 ataagggcca cttcttgggt cagacgtttt actttatcat tttgaagtga aatattatta
      541 gtcagtgaaa aaataatagc taaactcaaa caaaaaccta aaaagaataa catattggtt
      601 ggtgaatgaa tacccaggaa gtgagac
//
LOCUS       DAUELZ010000228          592 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.229, whole genome shotgun sequence.
ACCESSION   DAUELZ010000228 DAUELZ010000000
VERSION     DAUELZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.229"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
BASE COUNT          193 a           85 c          123 g          191 t
ORIGIN      
        1 caatcaatta tccctttcgg aaaaatcggt aaacatgtta tctatcgtag ctttggtagc
       61 gtcattattt gtttttatcg ataaaagaaa atagtgatta tgccaatgaa tttagatatc
      121 actcttagct ttttaaaata caaaagagtg aaattagaat gaaaaagact tcaaaaatat
      181 ggaagtgcta aaaaaatggt ctgactatca taaatggagg cggaaaagac ggagcaaata
      241 tctttttgac agggatggca ggtgctgcgc agggagtgac agtttgtgct cagacaggcg
      301 tatttatccc ttggcaaggt tatattttgt gtggagcagc tggtgctgct acaaatatta
      361 tctggcctca ctaaatatat aaatgactat ctatcactga tatataagta gtagaggagt
      421 ggtatgaaaa ttttagatat tatttttaac ctatatgata gaaagatgat atttctagaa
      481 aatgttgaaa agctgggagg ttacattgtc ccttttaccc ttatcatctt gagtgtagcc
      541 tacaacttta ggcaccctta tcctatttgg gctgctgtac taggaggtat at
//
LOCUS       DAUELZ010000229          572 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.230, whole genome shotgun sequence.
ACCESSION   DAUELZ010000229 DAUELZ010000000
VERSION     DAUELZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.230"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..>572
                     /locus_tag="VHI09_001746"
     CDS             <1..>572
                     /locus_tag="VHI09_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEQ6121570.1"
                     /translation="PKKLEVATIIFILVFGTISAIISPINDIPDEYVHYSRTVYISEG
                     DINLTNNNKKLRISKDVDKLIKQSGKTFITSNLKATKHSTREYSYPYIKGTNAYYSFS
                     YIPQALGILVGNALDLPILLTYYFGRLCNLISYAMLAFIAIKLSGSFKQVIAVVTLLP
                     MNIYLAASFNQDGFAIGLVLVTIGLFINLLS"
BASE COUNT          190 a           90 c           87 g          205 t
ORIGIN      
        1 ccaaaaaagt tagaggttgc tacgatcatt tttattttag tatttggaac gatatctgct
       61 atcatatcac ctataaatga tattcctgat gaatatgttc attattcaag gacagtttat
      121 atctcagaag gtgatattaa tcttaccaac aataacaaaa agttaagaat atccaaagat
      181 gttgataagc ttattaaaca gtcaggaaaa acatttataa catcaaactt gaaagcaact
      241 aaacacagca caagagagta tagctatcct tacatcaaag gaactaatgc atattatagt
      301 ttttcttata taccacaagc tttgggtatt ttagtaggaa atgcgctgga tttacctata
      361 cttttaacat attatttcgg aagattgtgc aaccttatta gctacgctat gcttgctttt
      421 atagctatca agttgtctgg ttcttttaaa caagtgatag ctgttgttac tttactacct
      481 atgaacattt atttagcagc atcttttaat caagacggct ttgctatcgg tttagtttta
      541 gtaacaattg gattgtttat taacttgctc tc
//
LOCUS       DAUELZ010000230          564 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.232, whole genome shotgun sequence.
ACCESSION   DAUELZ010000230 DAUELZ010000000
VERSION     DAUELZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.232"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..526)
                     /gene="rgg4"
                     /locus_tag="VHI09_001747"
     CDS             complement(<1..526)
                     /gene="rgg4"
                     /locus_tag="VHI09_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HEQ6121571.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
                     LILLELYLAYLDIEG"
BASE COUNT          179 a          115 c           67 g          203 t
ORIGIN      
        1 ttccctcaat atcaagataa gctaaatata attctagaag tattaaatca ttttgcctaa
       61 agcgtacctt agtctttatt tgattaaaat attcttgaag taagtcaatg ccaaagttag
      121 tattctcact caataaagta tctaaagttg cctgtaaaca atcaatgatt attttctctt
      181 cctctggtaa tttatcataa taatcctcaa aaatttcatc aaactgctct tctcgtactt
      241 gtaattttcc atcatccatg taagttggtg tccttaagat aaggtatttt aattctttgt
      301 aacgcttagg tagttctaaa tcagcaccat cagttaagta accgacacta acgtttaagg
      361 cttttgcaat aaaaataacc ttacttaaac ttggtatgga ttgacctagt tctattcgtg
      421 ctaattgacg aacagaaagt tcagactcat ccccacacaa gtcctcgcga ctaatcctct
      481 tttcaagtct taacactttt acttttccac caaaatgttc taacatggtt tctctccaac
      541 aacagttttc tttattatat caca
//
LOCUS       DAUELZ010000231          561 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.233, whole genome shotgun sequence.
ACCESSION   DAUELZ010000231 DAUELZ010000000
VERSION     DAUELZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.233"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..>561)
                     /locus_tag="VHI09_001748"
     CDS             complement(<1..>561)
                     /locus_tag="VHI09_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEQ6121572.1"
                     /translation="GFLIIYIYTQPKYAIELMTTLGEVALGSQTIFNILFMPAFVMNL
                     LILFFRPHITQMAIALIRGQIKEFNKIQVQLFAYLGVFSLIALVGSGLFGIPFLSILY
                     GTNLTDYWVDFMLIMLGGSIGSFATVIDNILTAMRKQQLLLIPYTGGFLISLLITNLF
                     VMKYHILGAALSFLITMLVWLGLSIM"
BASE COUNT          232 a           99 c           74 g          156 t
ORIGIN      
        1 tcataatgga taatcccaac caaaccaaca ttgttattaa aaagctcaaa gcagctccta
       61 aaatatgata tttcataaca aaaagattgg taattaataa cgaaatcaaa aatcctcctg
      121 tatatggaat aagaagaagt tgctgttttc tcatagcggt taagatatta tcgataactg
      181 tcgcaaaact accaatcgag cctcctagca taatcaacat aaaatctacc caataatccg
      241 taaggttagt accatatagt attgataaaa agggaatacc aaacaaacca cttccaacta
      301 acgctatcag tgaaaaaact cctaaataag caaacaattg aacttgtatc ttattaaact
      361 cttttatttg acctctgatt aaagcaatag ccatctgtgt aatatgaggt ctaaaaaata
      421 aaattagcaa attcataaca aaggctggca taaataaaat attaaaaatt gtttgagagc
      481 ccaaagcaac ttcacctaaa gtcgtcatta gttcaatagc gtattttggt tgagtataaa
      541 tatagataat caaaaaaccg t
//
LOCUS       DAUELZ010000232          537 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.234, whole genome shotgun sequence.
ACCESSION   DAUELZ010000232 DAUELZ010000000
VERSION     DAUELZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.234"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..501
                     /locus_tag="VHI09_001749"
     CDS             <1..501
                     /locus_tag="VHI09_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEQ6121573.1"
                     /translation="RISDGNWIDFRNKGKRSELGMPEPIYWCHGATGIGLTRYRESKW
                     LNDKELKNNYEMAKQTVLNNGYLNSDCLCHGKMGNMELFMNLDDSLKNEVDIEGIILN
                     IVRNSQKFGWESGLPQHTRVFNMMVGEIGIAYQLLRYISNYEVPSLLLLDVPKGSIEN
                     EKDYTD"
BASE COUNT          197 a           61 c          112 g          167 t
ORIGIN      
        1 cgtatttctg atggaaattg gattgatttt agaaataaag gaaaacgttc agagttagga
       61 atgccagaac ctatttattg gtgtcatggt gcaacaggta tcggattaac gagatataga
      121 gaatcaaaat ggttaaacga taaagagttg aaaaataatt atgaaatggc taaacaaacg
      181 gtattaaata atggttattt gaattcggat tgcctttgtc acgggaaaat gggcaacatg
      241 gaattattta tgaatttaga tgactccttg aaaaatgaag tagatataga aggtatcata
      301 cttaatattg ttcgtaattc tcaaaaattt ggttgggaat ctggcttgcc acaacacaca
      361 agagtgttta atatgatggt tggtgaaata gggatagcat accagttact tagatatata
      421 tccaattatg aagtaccatc attattgtta ttagatgttc caaaagggag tattgaaaat
      481 gaaaaagatt acaccgattg aacaaacaac tcctactgaa tgtggattat gttgttt
//
LOCUS       DAUELZ010000233          523 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.235, whole genome shotgun sequence.
ACCESSION   DAUELZ010000233 DAUELZ010000000
VERSION     DAUELZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.235"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(82..165)
                     /locus_tag="VHI09_001750"
     tRNA            complement(82..165)
                     /locus_tag="VHI09_001750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(129..131),aa:Leu,seq:caa)
     gene            complement(185..256)
                     /locus_tag="VHI09_001751"
     tRNA            complement(185..256)
                     /locus_tag="VHI09_001751"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(222..224),aa:Gln,seq:ttg)
     gene            complement(265..337)
                     /locus_tag="VHI09_001752"
     tRNA            complement(265..337)
                     /locus_tag="VHI09_001752"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(302..304),aa:His,seq:gtg)
     gene            complement(349..419)
                     /locus_tag="VHI09_001753"
     tRNA            complement(349..419)
                     /locus_tag="VHI09_001753"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Trp,seq:cca)
     gene            complement(426..506)
                     /locus_tag="VHI09_001754"
     tRNA            complement(426..506)
                     /locus_tag="VHI09_001754"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Tyr,seq:gta)
BASE COUNT          150 a          130 c          115 g          128 t
ORIGIN      
        1 gtaatataag agattgtact acgtagctac agcctaagat caaaacaaaa aacaaggttt
       61 acaaaccttg tttttcaagt ataccggcgg ccggggtcga accggcacgt ccttgcggac
      121 actggatttt gagtcctgcg cgtctgccaa ttccgccacg ccggctttaa atataaaatt
      181 taaactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
      241 gcttggctat accccaataa aataggcgag tgatgggaat cgaacccacg aatgtcagag
      301 ccacaatctg atgtgttaac cacttcacca cacccgccat atttctaaca cgggcagtag
      361 gaatcgaacc cacactgaag gttttggaga ccttagttct acctttaaac tatgcccgtt
      421 taggatggaa agagagggat tcgaaccccc gaacccgaag gagcggattt acagtccgcc
      481 gcgtttagcc tcttcgctat ctttccgtaa aatattaaat ttt
//
LOCUS       DAUELZ010000234          473 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.236, whole genome shotgun sequence.
ACCESSION   DAUELZ010000234 DAUELZ010000000
VERSION     DAUELZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.236"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..>473
                     /locus_tag="VHI09_001755"
     CDS             <1..>473
                     /locus_tag="VHI09_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEQ6121574.1"
                     /translation="VIKSTVPIGFTEYLRNRFHYNDIIFSPEFLREGSTIHDQLYPSR
                     TIVGNESRNSQLFLDILTDISVEKDSPSLLVGSSEAEAIKLFSNAYLAQKIAFFNELD
                     TFAEMQNLDSKKIIEAMGYDQRIGNSHNNPSFGFGGYCLPKDIKQLEYHFKEIPA"
BASE COUNT          159 a           73 c           84 g          157 t
ORIGIN      
        1 tgtaataaag tcgacagttc caattggttt tacagaatat ttaaggaatc gatttcacta
       61 caacgatatc attttttcac ctgagttcct tagggaagga tcaactattc atgatcaatt
      121 gtatccttcg agaactatag ttggaaatga atctagaaat tctcaattat tcttagacat
      181 actaacagat atatcggttg aaaaagactc gccatcttta ttagttggct cttctgaagc
      241 agaagcgata aagttatttt cgaatgcata cttagcacaa aaaattgctt tttttaatga
      301 gttggatacg tttgctgaaa tgcaaaattt ggactcaaaa aaaattattg aggctatggg
      361 atatgaccag agaataggaa attcgcacaa taatccttct ttcggttttg gtgggtactg
      421 tcttcctaag gatattaagc aattagagta tcattttaaa gaaattccag cac
//
LOCUS       DAUELZ010000235          430 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.237, whole genome shotgun sequence.
ACCESSION   DAUELZ010000235 DAUELZ010000000
VERSION     DAUELZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.237"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
BASE COUNT          139 a           73 c           66 g          152 t
ORIGIN      
        1 ttgatggtac tggttctaaa acttctagcg aggttataac ttttttcacc aacagtaatg
       61 tcaatgtcat ctttcttgtg aaaagtatcc atttcagttt catcagaagc caaaaaattt
      121 tttctttgaa tattgaaatt tgcgaatgtc ttggaaagtc taagacagct ggattgtatt
      181 gaaattaagt catcgtccga aaaagaatac attcttttat ctacagtttc caaatctcta
      241 aataaaatag agctgaagta gtggtaaaac atattttcaa caaacttggc aatatctcca
      301 aaaataattt ctttatagtc cgagtcggtt tgagtattca aataaagcaa tattgaagcg
      361 gtaacttgtc gctttccctt ttcaacttgg ctaacttgac ctgcattacc taggtcactt
      421 tactttaaat
//
LOCUS       DAUELZ010000236          428 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.238, whole genome shotgun sequence.
ACCESSION   DAUELZ010000236 DAUELZ010000000
VERSION     DAUELZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.238"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(1..428)
                     /locus_tag="VHI09_001756"
                     /pseudo
     CDS             complement(1..428)
                     /locus_tag="VHI09_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
BASE COUNT          150 a           80 c           51 g          147 t
ORIGIN      
        1 acttctggtt tatagctact cacttctatg tcagagataa tacaactctc aatcagttga
       61 ggtaatagat cttgaactac tgacaaatac aaagaaaagt tattttcaat cgaatcacaa
      121 tttatccgaa ttcttatatg atcattggta tcattatatc tcatataaaa catgttaaat
      181 caaccaacaa tctcaaaacc ctgctttggc tatcggaatt tagcatatta ttcatggtaa
      241 atttcaactc atttttccca tcgatagttg taaaatgaaa cttaccatca ctatatctta
      301 tataaatatc ctccaatcgc agttcgttct ctgaatccca tgatgtggta aaaaatgaag
      361 tattataaga ctcatcacta taattttgaa ttacattcgc aagtcttatc tgtttaggaa
      421 caaatgac
//
LOCUS       DAUELZ010000237          402 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.239, whole genome shotgun sequence.
ACCESSION   DAUELZ010000237 DAUELZ010000000
VERSION     DAUELZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.239"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
BASE COUNT          125 a           76 c           63 g          138 t
ORIGIN      
        1 aactagctct ctcaaaaaag attaaaaata aaggcattct aaagaatcat ttaagaaaac
       61 tttaaggtta ctatcgtaga atgaaaacat cctaaaaagc ggtttaccta aaccgttggg
      121 agaataggtt ttccccttgt tttccctttt cataaatctc ctgggcacct atcttcttag
      181 gtgccctccc ttgtttaaac ctgaccagtt aaccggctgg ttaggttttt aaatttaaaa
      241 agtcaacagc aaaaatatga tatcacccgc gtgttcttag ctctctcaat tatgggctaa
      301 aaacacgcat caatcaatct attgatgaag tttgctgctt tttgatgatc tttattgtga
      361 ctatattagt aatcctattg attttagttg acttttacgg ta
//
LOCUS       DAUELZ010000238          398 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.240, whole genome shotgun sequence.
ACCESSION   DAUELZ010000238 DAUELZ010000000
VERSION     DAUELZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.240"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            128..>398
                     /locus_tag="VHI09_001757"
     CDS             128..>398
                     /locus_tag="VHI09_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEQ6121575.1"
                     /translation="MRLDKYLKVSRLIKRRSVAKEVADKGRIKVNGILAKSSTNVKLN
                     DHIEISFGNKLLTVRVIEIKDSTKKEDALKMYEIISETRITLNEEA"
BASE COUNT          169 a           45 c           75 g          109 t
ORIGIN      
        1 atgacaatgg aacgaactag ctctttatac ttttgtcaaa aatctaagat ctttgctgcg
       61 aaattaggat cttttctacc atttaaaaaa taaaaatgat aaaatgaaga ggattgtagg
      121 agaaattatg agactagata aatatctaaa ggtatcgcgc cttattaaac gtcgttcagt
      181 agcaaaagaa gttgcggata agggacgaat taaagttaat gggatacttg ctaaaagttc
      241 aacgaatgta aaactaaatg atcacattga aattagtttt ggaaataaat tactgacagt
      301 aagagtcatt gaaataaaag atagtacaaa aaaagaagat gctcttaaga tgtatgagat
      361 aatcagtgaa acaaggataa cattaaatga agaagcct
//
LOCUS       DAUELZ010000239          386 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.241, whole genome shotgun sequence.
ACCESSION   DAUELZ010000239 DAUELZ010000000
VERSION     DAUELZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.241"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..>386
                     /locus_tag="VHI09_001758"
     CDS             <1..>386
                     /locus_tag="VHI09_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEQ6121576.1"
                     /translation="LKVDSLAEYEGDFNILSETARESIERLAEMFVKLNTNVKRRGGR
                     KNSLEEYQLKFIGKYGENCSIPFVEVINKDAGIGFPEYYKGGEGEAIELSDPIMQMFE
                     KKYEEALLNGGHIEFYSKDLDDFQTD"
BASE COUNT          152 a           41 c           87 g          106 t
ORIGIN      
        1 ttaaaagttg attcattagc agagtatgaa ggcgatttta atattctatc tgaaacggct
       61 cgggaaagta tagaacgact cgcagagatg tttgtaaaat taaatacaaa tgtaaagaga
      121 agagggggga ggaaaaattc acttgaagag tatcagttga aatttattgg caaatatgga
      181 gaaaactgct caattccctt tgttgaagta ataaataaag atgcaggcat aggttttcct
      241 gaatattaca aaggaggaga aggtgaagca atagagttaa gcgatccaat aatgcaaatg
      301 tttgaaaaga aatatgagga agctctatta aatggagggc atatcgaatt ttattcaaaa
      361 gatttagatg attttcaaac agatca
//
LOCUS       DAUELZ010000240          377 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.242, whole genome shotgun sequence.
ACCESSION   DAUELZ010000240 DAUELZ010000000
VERSION     DAUELZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.242"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..>377)
                     /locus_tag="VHI09_001759"
     CDS             complement(<1..>377)
                     /locus_tag="VHI09_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ6121577.1"
                     /translation="FKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKAGDKIAVFS
                     VPFDWNYLSEGKVIAYTYGGMTPYQEEPISKNIPVNLWINGKQISVTYNEISTNKTTV
                     TAQEIDLKVRKFLIAQHQLYSSGS"
BASE COUNT          114 a           62 c           61 g          140 t
ORIGIN      
        1 gaaccagaag aatataattg atgttgtgct attaaaaatt ttctaacctt tagatcaatt
       61 tcttgagctg taactgttgt tttgttagtt gatatttcgt tgtaagtaac agagatctgc
      121 tttccattaa tccataaatt aacagggata tttttagata ttggttcttc ttgataaggc
      181 gtcattccac cataggtata tgctatgact tttccttctg acaagtagtt ccaatcaaat
      241 gggacggaga acacagctat tttatctcca gctttaaaat cattggctgc atattcgtcc
      301 atttcggagt taataaaaaa atctctagca tctctaacat caagtttctt agtcactaag
      361 ttatgactgg ttttaaa
//
LOCUS       DAUELZ010000241          359 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.243, whole genome shotgun sequence.
ACCESSION   DAUELZ010000241 DAUELZ010000000
VERSION     DAUELZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.243"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(61..>359)
                     /locus_tag="VHI09_001760"
     CDS             complement(61..>359)
                     /locus_tag="VHI09_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ6121578.1"
                     /translation="YETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLENYRMIPT
                     TFENEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGNDVATANIKS"
BASE COUNT          107 a           69 c           54 g          129 t
ORIGIN      
        1 gtctagctga ctttgtctgc tagatctcac ctgttaaaca aaaaaggaca agtgtccttt
       61 ttaagattta atattggcag tggcaacatc atttccaaac catttctcag aaatagcttg
      121 aaatttgcca ttttgataaa gcaccctgaa agcacgatta atttttgctt gcaacgtttt
      181 gtcttctttt ctaagtccaa ccgaaaatgc ttcattttca aaggtcgttg ggatcatccg
      241 ataattctcc aattgccctt cttttgctaa atagtaattg gcatatactt tgtcaatcaa
      301 tattccatcg atacgatctg attttaaatc aataaaagct tgtgtaaagg tttcatatt
//
LOCUS       DAUELZ010000242          341 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.244, whole genome shotgun sequence.
ACCESSION   DAUELZ010000242 DAUELZ010000000
VERSION     DAUELZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.244"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            56..286
                     /locus_tag="VHI09_001761"
     CDS             56..286
                     /locus_tag="VHI09_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEQ6121579.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETRIDGYQLQIGKRVVGEIAELDE
                     KFAVIKNDTVDSFFKTLEQAVENIIENYNLNH"
BASE COUNT          131 a           38 c           69 g          103 t
ORIGIN      
        1 ttgctcttgc aagctttttt gattttgtgt aaaatagaca aagtgaggta aagtgatgag
       61 taaaaaagat aaaaaaattg aaatccaact gattgatcac aaagttatgg ttaatgagac
      121 taggattgat ggctatcaat tacaaattgg taaacgtgtg gttggagaaa ttgcagagct
      181 tgatgagaaa tttgcggtaa taaaaaatga cactgtggat agcttcttta aaacccttga
      241 gcaagcggtt gaaaatatca ttgaaaacta caatttgaat cattaaaagt aggtaaaaac
      301 acttgccagc ttacaaaaac tatgatagaa tagtttttgt t
//
LOCUS       DAUELZ010000243          337 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.245, whole genome shotgun sequence.
ACCESSION   DAUELZ010000243 DAUELZ010000000
VERSION     DAUELZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.245"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..>337
                     /locus_tag="VHI09_001762"
     CDS             <1..>337
                     /locus_tag="VHI09_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEQ6121580.1"
                     /translation="GRLKYFKEGISLYYIGEYLAECLLIEYSDTLLIHAAAVYNPLSG
                     HSILLLGDKGAGKTTVAMRLCIEHGYHLIGNDQVIFGSNSGILLTYAGTSFFKIRRTA
                     VLSDNLLFK"
BASE COUNT          109 a           47 c           65 g          116 t
ORIGIN      
        1 atggaagatt aaagtatttt aaagaaggaa tttcattata ctacattgga gagtacttag
       61 ctgaatgtct cttgattgaa tatagtgaca ccttattaat tcatgcagca gcagtttata
      121 atcctttatc tggccattca attttactat taggagataa aggtgctgga aaaactacgg
      181 ttgcaatgag gctttgtata gaacatgggt atcaccttat tggtaatgac caagtaatat
      241 ttggttcgaa ctctggaata ctactaactt atgcagggac atctttcttt aaaataagaa
      301 ggacagctgt tttgtcggac aatttactgt ttaaatt
//
LOCUS       DAUELZ010000244          332 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.246, whole genome shotgun sequence.
ACCESSION   DAUELZ010000244 DAUELZ010000000
VERSION     DAUELZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.246"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            <1..>332
                     /locus_tag="VHI09_001763"
     CDS             <1..>332
                     /locus_tag="VHI09_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEQ6121581.1"
                     /translation="FSIDYHYYLTTTGSCIDFGEEGAVGRGNKTHGIISSFRPNTMEA
                     PAGKNCTYFVGKVWGFLSDTIAKEIYEAFNTPCQIIMQLNIGSKLYRPTHLFIQTEES
                     VDQERVLE"
BASE COUNT          114 a           45 c           62 g          111 t
ORIGIN      
        1 gcttttctat tgattaccat tattatttaa ctactacagg ttcttgtatt gatttcggag
       61 aagaaggtgc agttggtcga ggtaataaaa ctcatggtat catatcaagt tttaggccaa
      121 atactatgga agcaccagct ggaaaaaatt gtacttattt tgtaggaaag gtttggggat
      181 ttttgtctga tactattgcc aaagaaattt atgaagcatt caatactccc tgtcaaatca
      241 ttatgcaatt aaatattgga tcaaaattgt atagaccaac ccatttattt attcagacag
      301 aagaaagtgt tgatcaagaa agagtattag aa
//
LOCUS       DAUELZ010000245          324 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.247, whole genome shotgun sequence.
ACCESSION   DAUELZ010000245 DAUELZ010000000
VERSION     DAUELZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.247"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            complement(<1..>324)
                     /locus_tag="VHI09_001764"
     CDS             complement(<1..>324)
                     /locus_tag="VHI09_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ6121582.1"
                     /translation="IIDQKILLAILLTEKELTMAAACSQTTLTALKIKQYLRQFNALF
                     KGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKHKSIAVYTRK
                     QGISQS"
BASE COUNT          107 a           51 c           55 g          111 t
ORIGIN      
        1 tgactgggat atcccttgtt ttcgtgtata aacagcgatt gacttgtgtt tgggattatc
       61 tagtaataag aatgtcaaca tttttagagt atctgataag gcaaaaatat cataaaaaaa
      121 tgcttctcgc ttatcattta atacctcaca ataaatacga gatttaataa gctcaatacg
      181 taagtaccct ttaaataagg cgttaaattg tcgaagatac tgctttattt ttaaagcagt
      241 caaagttgtt tgagaacaag ctgctgccat cgtcagttct ttttctgtca acaaaatagc
      301 cagtaggatt ttctggtcaa taat
//
LOCUS       DAUELZ010000246          315 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.248, whole genome shotgun sequence.
ACCESSION   DAUELZ010000246 DAUELZ010000000
VERSION     DAUELZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.248"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
BASE COUNT          121 a           61 c           31 g          102 t
ORIGIN      
        1 cctgcaaacc atcatatgtt ttaataagtt caaaagcgga aacaattatc ccattaaaaa
       61 aaatactcat taaaagaacg ataagttcat tgtcataatt aacaatataa attggtatca
      121 cagctaaagc cattccaaca ataacactaa gtaacgatgc ttttttggac cttttcataa
      181 aatcaccctt tttctttcaa tctactatac atattactcg attcaatcat cctcaacaaa
      241 ggccgttaaa acagactaat ttctcaaagt cctatttaag ggactttcta aatagacgtt
      301 gtttttttaa aaaat
//
LOCUS       DAUELZ010000247          313 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.denovo.249, whole genome shotgun sequence.
ACCESSION   DAUELZ010000247 DAUELZ010000000
VERSION     DAUELZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.denovo.249"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
BASE COUNT          119 a           56 c           41 g           97 t
ORIGIN      
        1 ttagatagta tttataactt aaaaaatcat atttattgct aggtaaacaa aatagatgta
       61 tttaactact tgtcaaacag tcttgattcc aacttttaaa tattcgtata tgaagtaaag
      121 ctactgtgca tgaaacaaaa aatataacaa taagtggaac aattgccatt ttctgttggt
      181 tatatagagt atctgatatt cttaaaagcc atgcccatgg aataagaaac cataccttat
      241 caccaaggct cgtttcacaa aatgctgcag caataacacc taccattgaa atacctaatg
      301 ccacacctgt act
//
LOCUS       DAUELZ010000248        13000 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS31078
            SAMN14440436-rid18295183.guided.046, whole genome shotgun sequence.
ACCESSION   DAUELZ010000248 DAUELZ010000000
VERSION     DAUELZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14440436
            Sequence Read Archive: SRR11403327
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001731493.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001731493.2
            Genome Coverage       :: 232x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/07/2023 10:08:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,778
            CDSs (total)                      :: 1,744
            Genes (coding)                    :: 1,677
            CDSs (with protein)               :: 1,677
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 28
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 36 of 67
            Pseudo Genes (incomplete)         :: 30 of 67
            Pseudo Genes (internal stop)      :: 18 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13000
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14440436-rid18295183.guided.046"
                     /strain="MGAS31078"
                     /serotype="emm4"
                     /isolation_source="patient clinical specimen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Iceland: Reykjavik"
                     /collection_date="2001-08"
                     /collected_by="Department of Clinical Microbiology,
                     Lanspitali University Hospital"
                     /note="genotype: emm4"
     gene            123..785
                     /locus_tag="VHI09_001765"
     CDS             123..785
                     /locus_tag="VHI09_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEQ6121583.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDNSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            766..2451
                     /locus_tag="VHI09_001766"
     CDS             766..2451
                     /locus_tag="VHI09_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEQ6121584.1"
                     /translation="MTFSWTKLVPLSKIQFAFLILVLFYQIHSPSWLTFLLSLSLICL
                     LVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLEIQPVQITSVALVPDSIRINGDQ
                     LAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
                     FLAYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLL
                     FGYLDTSFGEMTDYYSQLGIIHLFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELP
                     FACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLT
                     FSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTALLSYLF
                     DLFILPLLCLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLIL
                     VISFAIFYDYRQVRQRVITCGLVIALTLLSVKYPLTNEVTFIDIGQGDSILVREWTGK
                     NLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHAEV"
     gene            2556..3212
                     /locus_tag="VHI09_001767"
     CDS             2556..3212
                     /locus_tag="VHI09_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HEQ6121585.1"
                     /translation="MMKRKIRMTTIFLTRTSCSNCGKQSTFERFDRVYAAKTPEIISA
                     ILDWDFFKFTCHNCNHKVLIDYPTVVVDEEQKTIIQYCADGNVDVLSMQICSLISEGV
                     NLSEYRIRVVSDIESFVEKVQIVSVGYDDRAIELMKYMNSPLEDGDIQFNYEHMVFTK
                     VGHENYQFMFINNQIAVASLDFSQEQYEYYLADVEDLETNTYYIDSRWAESFFRTSLA
                     "
     gene            3341..4453
                     /locus_tag="VHI09_001768"
     CDS             3341..4453
                     /locus_tag="VHI09_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ6121586.1"
                     /translation="MVRRNSKITRQQKKIRDAFVSERTVEIIPAKREFTDVKTKKLRV
                     AAYCRVSTFDESQSGSFELQKQTYTERINSNPDWIMAGIYADQGASGTSIKRREQFQQ
                     MLHDCRCGKIDLIIVKSVSRFARNQLDFISIYRELKALSPPVGIYIEDINLNTLDTNS
                     EFILGIMAIVAQGESEQKSASITWSVIERFKRGIPMIPTHNLLGYTKDQYGRVVIDET
                     EAKIVRLIYDSYIEGMTASEIASTLMTNHIPTVTGLERWTSLAVYNILRNEKYKGEII
                     MQKTYTVDCFSHKTRKNNGEKPKYRLKNGIPSIIPESRWDLVQELLKQPRRKSKSTSE
                     IFVPKLYIKKLKSGKLRDFVVLDPSWKSEDIHEVFK"
     gene            4468..4884
                     /locus_tag="VHI09_001769"
     CDS             4468..4884
                     /locus_tag="VHI09_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121587.1"
                     /translation="MRFKDFNLEVVNVERYSSDYSMTVNKNFVTFSKGIVQALEYPAH
                     VLVAFNKDTKVMGIQVCRAKTRGAFSFSKPVGEQKGIVQVGHKTLKETLLTIMSEWKS
                     DKRYRVEGIHIPEDKAFVFELKDFDELSDFRKNDNR"
     gene            4994..5205
                     /locus_tag="VHI09_001770"
                     /pseudo
     CDS             4994..5205
                     /locus_tag="VHI09_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368983.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
     gene            5714..6931
                     /gene="mef(A)"
                     /locus_tag="VHI09_001771"
     CDS             5714..6931
                     /gene="mef(A)"
                     /locus_tag="VHI09_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HEQ6121588.1"
                     /translation="MEKYNNWKLKFYTIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLLGFLPYAVFGPAIGVLVDRHDRKKIMIGADLIIAAAGSVLTIVAFYMELPV
                     WMVMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAAL
                     LYSVWELNAIIAIDVLGAVIASITVAIVRIPKLGDRVQSLDPNFIREMQEGMAVLRQN
                     KGLFALLLVGTLYMFVYMPINALFPLISMDYFNGTPVHISITEISFASGMLIGGLLLG
                     LFGNYQKRILLITASIFMMGISLTISGLLPQSGFFIFVVCCAIMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLTGSIMSLAMPIGLILSALFADRIGVNHWFLLSGTLIICIAIV
                     CPMINEIRKLDLK"
     gene            7051..8514
                     /gene="msr(D)"
                     /locus_tag="VHI09_001772"
     CDS             7051..8514
                     /gene="msr(D)"
                     /locus_tag="VHI09_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HEQ6121589.1"
                     /translation="MELILKAKDISVEFKGHDVLDINELEVYDYDRIGLVGANGAGKS
                     TLFKVLLGELIPPGCKMNHLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKRQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEGGGRLAHQKSIGSKEKKMHNAAKSLENRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGTG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKIKLKH"
     gene            complement(8632..8931)
                     /locus_tag="VHI09_001773"
     CDS             complement(8632..8931)
                     /locus_tag="VHI09_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121590.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVRTLEENPLIYHYTYKKTTTYLAEK"
     gene            complement(8918..9285)
                     /locus_tag="VHI09_001774"
                     /pseudo
     CDS             complement(8918..9285)
                     /locus_tag="VHI09_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9282..10697)
                     /locus_tag="VHI09_001775"
     CDS             complement(9282..10697)
                     /locus_tag="VHI09_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEQ6121591.1"
                     /translation="MGYIDYSIEPQSDIAFLDMKSFYASVECVDRGLHPLYTSLCVMS
                     RADNSAGLILASSPMFKKVFGKANVGRSYDLPFDINTRKFSYQNAWKQGLEVTPKYKS
                     FIEHWAKRTLIVPPRMDRYIEKNLEIQHIFQDYAAPDDILPYSIDEGFIDLTSSLSYF
                     ISDKSMSRKDKLDNVSAMIQRDIYSKTGIISTVGMSNSNPLLAKLALDNEAKKTATMR
                     ANWSYEDVETKVWAIPKLTDFWGIGSKTEIHLQKLGIHSIKELANFNPDILKKEFGKV
                     GVQLWFHANGVDESNVHEPYKPKSRGLGNSQVLPRDYRTQREIEIVLAEMADQVANRL
                     RSAHKKATVVSIHIGYSRTEMKKSINAQKKIDPANLPKTMVGHVLELFRKKYTSGAVR
                     QIGVSYGGFVDENFTLLSLFDDVEQIEKENRLQTAIDVVREQFGFLAIQKGTVLTEGS
                     RNIERSKLIGGHSAGGLEGLK"
     gene            complement(10820..11047)
                     /locus_tag="VHI09_001776"
     CDS             complement(10820..11047)
                     /locus_tag="VHI09_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ6121592.1"
                     /translation="MKLKDILELGTYGFNPNCKVEIFCMDNFEERLENEGFDEILIPQ
                     NDDAKIYPYAFLIEDSILIAMTEVDDDINEC"
     gene            complement(11050..11739)
                     /locus_tag="VHI09_001777"
     CDS             complement(11050..11739)
                     /locus_tag="VHI09_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ6121593.1"
                     /translation="MFSHGRLKQRRLEIKLSQTAIANQLNVHRSAYHNWENGRNTPNQ
                     KNLTALAAILDVPVTYFESEYNIVNNYLQLSPDNQLKAEEYVEELLLSQQTSNVIPLF
                     SVQVLSDIQLSAGLGEGFFDEFETETIYSDEEQYGYDIAAWIEGDSMEPVYKSGEVAL
                     IRSNGFDYDGAVYALSWNDSVYIKKLYRDEDGFRMVSLNKDYPEKFIPYEDEPRIVGL
                     VVGHFMPVEGV"
     gene            complement(11853..12977)
                     /gene="dcm"
                     /locus_tag="VHI09_001778"
     CDS             complement(11853..12977)
                     /gene="dcm"
                     /locus_tag="VHI09_001778"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEQ6121594.1"
                     /translation="MKYLRKKYNLTQKELADALGLQKFGDRTIRRWEAEETHPSKLEQ
                     SSIQSFFNSLKNPPFIISENLPFKMIDLFAGIGGTRLGFQLTNEVETVFTSEWDKFAQ
                     KTYITNFGDTPEGDITQIDENTIPDHDILVAGFPCQAFSQAGKRLGFEDTRGTLFFDV
                     ARIIKAKRPKAFLLENVKNLKTHDKGKTYKIIISTLENLGYSTSTVLFKARDFGVPQN
                     RERIYIVGFDKTCVNNFSEFRFPEPFHLETKLGNILQTDVDEKYTISDKLWEGHQRRK
                     AEHKTKGNGFGYSLFNADSPYTNTLSARYYKDGSEILIEQKGKNPRKITPREAARLQG
                     FPEEFIIPVSDTQAYKQFGNSVCVPTIHEIAKQILAVLKK"
BASE COUNT         4029 a         2147 c         2568 g         4256 t
ORIGIN      
        1 aatcactttt ttcatttcta aaaaagatat tcgatgatac ccttaactct tttaagcaca
       61 aaaaatgaca atactactat acttttggat atcatttacg aataaaaaga tgaggtaaaa
      121 aaatgttaga cgaattatta attaaaggaa aatcgttttt gagagagcgg tattttctgc
      181 catacctaat tagtctttta ttaggacttt ttctaatact aagcttcagt tttttgttct
      241 ggggaaatag gcagagcaag gcagccgttc ctgctttgag agaaatatcg cctgtgaaac
      301 agcaggttag tgaagaaaaa aaggaaattc aagaagataa cagtatttta gtggatttaa
      361 aaggcgccgt acaaaaagag ggagtttata agctaactgc tagcagtcgt gtgcgagatg
      421 tcattgaact agctggaggc ctaacttcag aagctgataa acatgctatt aattttgcag
      481 aaaaactaac tgatgagcaa gttgtttatg tacctaaaca aggagaagag atttctgtgc
      541 taccaagatc attagtttct ggtaaaaaag aaactgccag caaggatcag tcaaaggttc
      601 atattaataa ggccagctta gaagaactac aacatatccc aggtattggt gctaaaagag
      661 ctcaggatat tattgatatg agagataagt taggtggttt taaagcgtta gaagatctcc
      721 gtcaggtttc ggggattggc gaaaaaacgc tcgagaaatt aaaagatgac attttcttgg
      781 actaagctag tgcccctgtc aaagattcaa tttgcttttt taatcttggt tctcttttat
      841 caaatccata gccctagctg gttaacgttt ctccttagcc ttagtttgat ttgcttactt
      901 gtcaagcgtt tgtctaaaaa agaatttctc ggtgtctttg ccatccttag tttctgtgct
      961 ttgtttttgc tgtatcagaa acagcagttg gttcaaaaac tggaaattca gccagtacaa
     1021 ataacctctg tagcattagt tcctgatagt attcggatta atggtgatca attagctgtt
     1081 cttggacgtc atgggaaaca ttcttatcag ttattttacc gtttgaaaag ccaagcagaa
     1141 gcccaattgt tcaagaaaga gcaccgttgg ttagtgatgc acgcaaaagt gacattggaa
     1201 aaagctgagg aagttcgaaa ttttaaaggc tttaattatc agactttttt ggcttatcaa
     1261 ggaatttaca ggatagggaa ggtagagcaa atagaacagc ttgaagtgat ttcacccgag
     1321 tcaatatgtg actatttgtc ctctttaaga cgacgagcca ttgttcattg ccagcagcat
     1381 tttccaagac ctatgtctca ctacctgacg ggcctccttt ttggttattt agatacgtcg
     1441 tttggtgaga tgacagatta ttatagtcag ttgggtatca ttcatctctt tgctttatca
     1501 gggatgcagg ttggcttctt tcttacttgt tttagaaggg tgttgctatt actcgcagtt
     1561 cctttggaat ggataaaatg gatagagctt ccttttgctt gcttttatgc cgcgttgaca
     1621 ggttatagta tttctgttat tcgaagcctc gtgcagtcgc agttgcgtca tttaggaata
     1681 aaaggattag acaatctcgc ttgtactttt ttacttgtct ttttatggga tgcccatttc
     1741 ttaatgactg ttggtggcgt attaacgttt agctatgctt ttttgttaac agtggttact
     1801 gttgaagaat tatcaggagc taaaagacag ttggtacaag tcttgaccat ttccctagga
     1861 atattaccat ttttgctctt ttatttttcc agttttaacc caatgtcaat ggtcttaaca
     1921 gctctcttgt cttatctctt tgaccttttt attttacctc ttctttgtct ggttttttgt
     1981 ctatcccctt tggtgactgt ctcaatatgc aaccatcttt ttattttatt agaaaaagtc
     2041 attcaatttc tcggaaatac tttcaatagt tcccttgttt ttggaagccc gaccagttgg
     2101 cacttgctta tcttggtgat tagcttcgct attttttatg attatcggca ggtgcgacaa
     2161 cgagttatca cttgcggttt ggttatagcg ttgacgcttt tgtctgtcaa atatcctttg
     2221 accaatgaag tgacctttat cgacattggc cagggagata gtattttggt gagggagtgg
     2281 acagggaaaa atctcttaat agatgtaggt gggcgccctt tcttttcttc aaaagagcac
     2341 tggcgacgag gacatcacgt agctaatgct cagaaaacct taattcctta cttaaaaagt
     2401 agaggtattc acacaattga ccaactatta gtaactcacg cagaagtgtg agttctaaaa
     2461 ttgatacaat acaatttaac ttttttttga aaaaaattct aaaatttatt ttgcattgcg
     2521 aatataataa gtgagagcaa gttgcttact taataatgat gaagaggaaa attagaatga
     2581 caacaatttt tttaacaaga acaagctgca gcaattgtgg aaaacaaagt acatttgaaa
     2641 gatttgatag ggtttatgcg gcgaagactc ctgagattat ttctgctatt ttagactggg
     2701 atttctttaa gtttacttgc cataactgta accacaaggt gctaattgat tacccaacag
     2761 tcgtagtaga tgaagaacaa aaaacaatta ttcagtattg tgcagatggt aatgttgatg
     2821 tactttcaat gcaaatatgc tctttgattt cagagggtgt caatctcagt gagtatagaa
     2881 ttcgagtagt ttcggatata gagtctttcg ttgagaaggt acagattgtt tcagtagggt
     2941 atgatgatag agcaattgag cttatgaagt atatgaatag tcctcttgag gatggtgata
     3001 ttcagtttaa ctatgaacat atggttttca ccaaggttgg acatgaaaac tatcaattta
     3061 tgtttattaa taaccagatt gcagtagcat ctctcgactt ttctcaagaa caatatgagt
     3121 actaccttgc tgatgttgaa gacctagaaa ctaacacata ttacatagac tctagatggg
     3181 cggaatcatt ctttcgtacc agtttggcat aaaattcaaa atctatttat aagtataaat
     3241 ggcctgattt ttggttaggt cattttttat cttctaattt agaaaaacgt agttaatata
     3301 tataattatt atgtagattt ctagaaagga ggtaaaacca atggtaagac ggaattctaa
     3361 aattacccgt caacagaaga aaattcgaga tgcatttgta tctgagagaa ctgttgaaat
     3421 tattcctgca aagcgagagt tcactgatgt taaaactaag aagttacgtg ttgctgcata
     3481 ttgtcgagta agtacttttg acgaatctca gtcaggtagt tttgaacttc aaaaacaaac
     3541 ctatactgaa agaatcaata gtaatcctga ttggataatg gctggaatct atgctgacca
     3601 aggtgcatca ggaacatcaa tcaaacgacg tgaacaattc caacagatgc ttcatgattg
     3661 tagatgtggg aagattgact tgataatcgt taagagtgtt agcaggtttg ctcgtaatca
     3721 gctagatttc attagtattt accgagagtt gaaggcacta tcccctccag ttggaatata
     3781 tatcgaagat ataaatttga atacacttga tacgaatagt gaattcattc ttggtattat
     3841 ggccatagta gctcaaggtg agagtgaaca aaaaagtgcg tcaattacat ggtctgtcat
     3901 tgaacgcttt aaaagaggga taccaatgat tcctacccat aaccttcttg ggtatacgaa
     3961 agaccagtat ggtcgagtag tgatagatga gacagaagca aaaattgttc gcctaatata
     4021 tgattcgtac atcgaaggta tgactgcaag tgagattgcc tctactttaa tgaccaatca
     4081 tataccaaca gttactggtt tggagcgttg gacaagttta gctgtttata atattctcag
     4141 aaacgagaaa tataaaggcg aaatcatcat gcagaagaca tatactgttg attgttttag
     4201 tcataaaaca cggaaaaata atggtgaaaa gccaaaatat cgattaaaaa atggaattcc
     4261 ctctatcata ccagaatcaa gatgggattt agtacaagaa ttactcaagc aacctagaag
     4321 aaaatcgaaa tctacttctg agatatttgt acctaaactc tacatcaaga aactcaaatc
     4381 tggtaagttg agggattttg ttgtacttga tcctagttgg aaatcggaag acattcacga
     4441 agtttttaaa taagaaagag gtaaattatg cgttttaaag attttaatct tgaagttgtt
     4501 aatgttgaac gttactcatc agattatagc atgacggtga acaagaactt tgttacattt
     4561 agtaaaggaa ttgtccaagc tttagaatac ccagcacatg tactggttgc ctttaataag
     4621 gatacaaagg taatgggtat acaagtttgt cgtgcaaaaa ctaggggagc attttctttt
     4681 tcaaaacctg ttggggagca aaaaggtatt gttcaggtag gtcataaaac tttaaaagaa
     4741 acactcttaa caatcatgag tgaatggaaa tctgataagc gttatagagt agaggggatt
     4801 cacattcctg aagataaagc ttttgttttt gaattgaagg actttgatga actatctgat
     4861 tttcgtaaaa acgacaatag atagtaagga acgaagataa aaattattgg taggagtaca
     4921 actattgtat tcctgccatt ttcatatcct tgcaaggggg ttcatgttaa aatatactgt
     4981 aggaggtaac tctatgaacg ataaagttac aaaagtcaga ctagcctatc ctttgggaga
     5041 agacctaatc tatgtattcc tgaaaaatat aatgcctacc gtagattcct actgggatgc
     5101 ttaggaacga atccactcgt ggctatatgt atgaatccct cagcagctaa tgaggactat
     5161 agcgatcgta caatcaaccg aattctaaat gctagtaaaa aaattatatg aagataagct
     5221 caccgttgaa ttcaagtcag ggattgagat tgacgaagag atatagaata ttaaccgcaa
     5281 tttaagggct aacccccttt ggcggttttt aatggatttt tttaatttat aagttgctta
     5341 aaatgactta ttatgttaaa atatatgata atccaaataa gaatttagga ggaaccgaaa
     5401 ctatgacagc ctcaatgcgt ttaagataag ctggcaataa gaaaagcaga atctataccc
     5461 gatgataggc ttttttgttg tgcttattta tacgatattg agcattcatt agttacggtg
     5521 aggatattgg ttatttaact atacctttat ttaactatac ctttatttaa ctatgtcttt
     5581 aatatgaatg tttccaaatt gtatgtatgc agaccaaaag ccacattgtg gatttaggcc
     5641 tgcatttttt tattgcctat aatgctattc aaaatagaaa tttaagcaaa ataatatgca
     5701 ggagataata taaatggaaa aatacaacaa ttggaaactt aagttttata caatatgggc
     5761 agggcaagca gtatcattaa tcactagtgc catcttgcaa atggcgatta ttttttacct
     5821 tacagaaaaa actggatccg cgatggtctt gtctatggct tcactattag gttttttacc
     5881 ctatgcggtc tttggacctg caattggtgt gctagtggat cgtcatgata ggaagaagat
     5941 aatgattggt gctgatttaa ttatcgcagc agctggttcg gtgcttacta ttgttgcatt
     6001 ctatatggag ctacctgtct ggatggttat gatagtattg tttatccgta gcattggaac
     6061 agcttttcac accccggctc tcaatgcggt tacgccactt ttagtaccag aagaacagct
     6121 tacgaaatgt gcaggctata gtcagtcttt gcagtctata agctatattg ttagtccggc
     6181 ggttgcagca ctcttatact ccgtttggga actaaatgct attattgcca tcgatgtatt
     6241 gggtgctgtg attgcatcta ttacggtagc aattgtacgt attcctaagc tgggtgatcg
     6301 cgtgcaaagt ttggacccaa atttcataag agaaatgcaa gaaggaatgg ctgtactacg
     6361 gcaaaataaa ggattatttg ctttattact cgttggaaca ttatatatgt ttgtttatat
     6421 gccaattaat gcactattcc ctttaattag catggattac tttaatggaa cacctgtgca
     6481 tatttctatt acggaaattt cctttgcatc tggaatgttg atagggggtc tattattagg
     6541 gttatttggg aattaccaaa agcgaatctt attaataacg gcatccattt ttatgatggg
     6601 gataagctta accatttcag gattacttcc ccaaagtgga tttttcattt ttgtagtctg
     6661 ctgtgcaata atggggcttt ctgttccgtt ttacagcggt gtgcaaacag ctctttttca
     6721 ggagaaaatt aagcctgaat atttaggacg tgtattttct ttaactggaa gtatcatgtc
     6781 tcttgctatg ccaattggat taattctttc tgcactcttt gctgatagaa tcggtgtaaa
     6841 tcattggttt ttactatcag gtactttaat tatttgcatt gcaatagttt gcccaatgat
     6901 aaatgagatt agaaaattag atttaaaata aacaatattg gagggatatt tatgtatctt
     6961 attttcatgt aattcttcct gctaaaatcg cagggttttt cctgcgtaca agcaaatgaa
     7021 agcatgcgat tatagacagg aggaaatgtt atggaattaa tattaaaagc aaaagacatt
     7081 agtgtggaat tcaaaggaca cgatgtttta gatataaatg aattagaagt atatgattat
     7141 gaccgtattg gtttagtagg agcaaatggt gcaggaaaaa gcactttatt caaggtactt
     7201 ttaggagaat taattccccc aggatgtaaa atgaatcatc tgggtgaact tgcctatatt
     7261 ccccagttgg acgaagtaac tctgcaggag gaaaaagatt ttgcgcttgt aggcaagcta
     7321 ggtgttgagc aattaaatat acagaccatg agcggtggtg aagaaacaag gcttaaaata
     7381 gcacaggcct tatcggcaca ggttcatggt attttagcgg atgaacctac gagccattta
     7441 gaccgtgaag gaattgattt tctaatagga cagctaaaat attttacagg tgcactgtta
     7501 gttattagcc atgaccgcta ttttcttgat gaaatagtag ataaaatatg ggaactgaaa
     7561 gatggcaaaa tcactgagta ttggggaaac tattctgatt atcttcgtca gaaagaggaa
     7621 gaacgtaaga gacaagctgc agaatacgaa caatttattg cggaacgtgc tcgattggaa
     7681 agggctgcgg aggaaaagcg aaaacaggct cgtaaaatag aacagaaggc aaaaggttct
     7741 tcaaagaaaa aaagtactga aggcggaggg cgtttagctc atcaaaaatc aataggaagt
     7801 aaggaaaaaa agatgcataa tgccgctaaa tccctagaga acaggattgc ggcattagga
     7861 aaagtagaag ctccggaagg cattcgcaga attcgtttca ggcaaagtaa agcattggag
     7921 ctccataatc cataccctat agtcggtgcg gaaattaata aagtatttgg ggataaggca
     7981 ctgtttgaaa atgcatcttt tcaaattccg ctaggagcaa aagtggcatt aacgggtggt
     8041 aatggaaccg gaaaaacaac tttaatccaa atgatcttaa accatgaaga aggaatttct
     8101 atttcaccta aggcaaaaat aggttacttt gcacagaatg gttacaagta caacagtaat
     8161 cagaatgtta tggagtttat gcagaaggat tgtgattaca atatatcaga aattcgttct
     8221 gtgctagcat ctatggggtt caaacagaac gatattggaa aaagcttatc tgttttaagc
     8281 ggtggagaaa ttataaaatt gttgcttgct aaaatgctca tgggtagata taacatccta
     8341 ataatggatg aacccagtaa cttccttgac ataccaagtt tagaggcttt ggaaatacta
     8401 atgaaggagt acaccggaac tatcgtgttt atcacccacg ataaacgatt actcgaaaat
     8461 gtagctgatg tagtttatga aattagagat aagaaaatta agctgaaaca ttaaatttta
     8521 ggtagtcgct ggtcagtata gtctgttctg gctggcgact ccattgttaa agagtataaa
     8581 gacttgagat tttatgaaga ttaaaaatag gaacagtaaa tcgaactgct cctatttttc
     8641 tgctaaatat gttgtagttt tcttatatgt ataatgatag attagtggat tctcttctaa
     8701 ggttcttact tcaaatatga agaagtgatc gcggttatct ctggactttt ccttattgag
     8761 gacaaagtaa ttcttacgtg aagtcgccat tgttttgagg atatcatcgg tcaggaaggt
     8821 caatggaata ttcatgttgg agtagcggta gaagtcacgt tcaaaatctt ggtagctctc
     8881 gctgtaatag tccatttgca ggtgattacg ctgaaattca agctgattca tagagcacct
     8941 cctctacaag ttcaatacta acaatgtctt tcaatttcaa attaatgtga cctgttgtag
     9001 tttttatcaa aatgaaatct ttggtcagac ttggtattgt tccagtatag gaaacttgat
     9061 tgttttttta acaacatgaa tgcgcgtgtt cagttgccca gcatacacct gactgaggag
     9121 taataatttc ttctccagtg acaaatctga catatacgtt actttgtttg catcgtcagt
     9181 gagtgctgat gtatgctcag ataggaaaaa gcccatccat ttttgcatct tagtatcctg
     9241 gtactctctt gctgactgaa atggcaagta tgaacgatca atcattttaa tccctccaat
     9301 ccaccagcgg aatgaccgcc gataagttta ctacgttcaa tatttctgga accttcggtt
     9361 aggacggttc ctttttgaat agctaaaaag ccaaactgtt ctctgacaac atcaattgct
     9421 gtctgaagtc tattttcttt ttcaatttgt tctacatcat caaaaagtga cagtagagta
     9481 aagttttcat ctacaaagcc accataagac acaccgattt gcctcactgc accagaggtg
     9541 tatttctttc ggaataattc aagtacatga ccaaccattg tttttggaag atttgctggg
     9601 tcaatttttt tctgagcatt aattgatttt ttcatctcag ttctagaata gccaatatga
     9661 atagaaacga cagtagcttt tttatgtgcc gagcgaagac ggttagcaac ctggtcagcc
     9721 atttcagcta atacgatttc aatttctctt tgggttctgt aatctctagg aagtacttgt
     9781 gagttaccca atcctcgtga ttttggttta tagggttcat ggacgttgct ctcatcaaca
     9841 ccgtttgcgt gaaaccatag ctgaacacca actttgccga attctttttt gagaatatca
     9901 ggattgaagt tggctagttc tttgattgaa tgaataccaa gtttttgtaa atgaatctcg
     9961 gttttactac caatccccca aaagtctgtt agttttggaa tggcccatac cttggtttct
    10021 acatcttcgt atgaccagtt cgctctcatt gtagcagttt ttttagcttc attatctaga
    10081 gctagtttag ctagaagagg attggaatta ctcattccaa cagttgagat aatacctgtt
    10141 ttactgtaaa tatctctctg aatcatagcc gaaacattat ctaacttatc tttccttgac
    10201 attgacttat cagaaataaa gtaagaaagt gagctagtaa ggtcaataaa gccttcatcg
    10261 attgaatagg ggagaatgtc atctggagca gcatagtctt gaaatatatg ctgaatctct
    10321 agattcttct caatatatct gtccattcgc ggaggaacga tgagtgtacg ctttgcccag
    10381 tgttcgatga aagatttata cttcggtgtt acctcaagac cttgtttcca cgcattctga
    10441 tagctgaatt ttcgagtgtt tatatcaaat ggcaagtcgt aggaacgacc tacatttgct
    10501 ttaccgaata cttttttaaa cattggagaa gaagcgagaa tcaatcctgc cgagttgtct
    10561 gcacggctca tgacgcacag tgatgtgtag agtggatgta aacctctatc cacacattct
    10621 acagaagcgt agaaggactt catatcgagg aaggctatgt cactttgagg ttcaatagaa
    10681 tagtcgatat agcccatagg attatctcct ttttagtttc tctagctctt cgataagttc
    10741 tccgatgtag tcagtgatgt cgtattcaaa tttgtcaggg gtgaagataa tacgttcgtc
    10801 tttgatgtaa atcatttctt taacattcat tgatatcgtc atccacctcg gtcatagcaa
    10861 tcaaaataga atcctctatc aaaaaagcgt aaggatagat ttttgcatca tcattttgag
    10921 gaataagaat ttcatcgaat ccttcattct ctagccgctc ttcaaagttg tccatacaga
    10981 atatttcaac tttacagttc ggattgaaac catacgtccc aagttctaaa atatccttta
    11041 gcttcatgac tatactccct cgacaggcat aaagtgacct acaactagac caacaattct
    11101 aggttcatcc tcgtaaggga tgaacttctc tggatagtcc ttattcaagg ataccattct
    11161 aaatccatcc tcatcacggt agagtttttt gatatagact gagtcattcc atgataatgc
    11221 atagactgca ccatcatagt cgaaaccgtt tgaacgaatt agtgcgactt ctccactctt
    11281 atatacaggc tccatagaat ctccctcaat ccaagcagca atgtcgtaac cgtattgttc
    11341 ctcatcagag tagattgttt cagtttcaaa ctcgtcaaag aatccttctc caagaccagc
    11401 tgagagttgg atatctgata gcacttggac tgagaagaga ggaatgacgt ttgaagtttg
    11461 ttgggaaaga agaagttcct caacatactc ctctgccttt agctggttat cgggagataa
    11521 ctgaagatag ttatttacga tattgtattc agattcaaaa taagtgactg gtacatcaag
    11581 aatagcagcg agagcggtga ggtttttttg attaggtgtg tttcttccgt tttcccaatt
    11641 atggtaagcg gaacgatgaa catttaattg attagcgatt gcagtctgtg ataactttat
    11701 ttcaagtctt cgttgtttta atcttccgtg agaaaacatg ttcccctcct ttttgtctag
    11761 ttataactca acaaaattat atcagaatat ttatattttg caactagaat taaaaaagtc
    11821 tatcaattca attgatagac tttatatatt aactattttt ttaggacggc tagtatttgt
    11881 ttagcaattt catgaattgt tggtacacag acagaatttc caaattgttt gtaggcttgt
    11941 gtatcagaaa ccggaattat aaactcttca gggaaacctt ggagtctagc agcttctcgt
    12001 ggagtaattt tacgtgggtt tttacctttt tgttctatta atatttcact tccatctttg
    12061 tagtatcgag cagaaagtgt atttgtatat ggactatcag cattaaatag tgagtagccg
    12121 aatccatttc cttttgtttt atgttcagct ttccttcttt ggtgtccttc ccagagttta
    12181 tcggagatag tatacttttc atcaacatca gtttgtagaa tatttcctag tttagtttct
    12241 aaatgaaacg gttcagggaa acggaattca ctaaaattat ttacacaagt cttatcaaat
    12301 ccaactatat aaatcctttc tctattttgt ggtacaccaa aatctctggc cttaaaaagt
    12361 acagttgatg tactatatcc taaattctca agagttgaaa tgattatctt gtatgtctta
    12421 cctttatcat gtgtctttag gtttttgaca ttttcaagta aaaaagcttt tggacgttta
    12481 gcttttatta ttctagcaac gtcaaaaaat aaagttccac gagtatcttc aaatcctaga
    12541 cgtttacctg cttgggagaa agcttgacaa ggaaaaccag caacgagtat atcatgatct
    12601 ggtatagtat tttcatcaat ctgagtgatg tcaccttctg gagtgtctcc aaaattagta
    12661 atgtatgttt tttgagcaaa tttatcccat tcacttgtaa atacagtttc tacttcattt
    12721 gttaattgga aacctaatct agttccacct attccagcaa aaagatcgat cattttgaac
    12781 ggaagatttt cactaataat aaaaggcgga ttctttagag aattaaaaaa agactgaata
    12841 ctggactgtt caagttttga aggatgcgtt tcttctgctt cccaacgtct aatggttcta
    12901 tctccaaatt tttgaagacc gagtgcatct gctaattctt tttgagtgag attatatttt
    12961 tttcgtagat attttatatc atttcccgaa agattttctg
//
