LOCUS       DAUGFP010000001        85177 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.001, whole genome shotgun sequence.
ACCESSION   DAUGFP010000001 DAUGFP010000000
VERSION     DAUGFP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 85177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85177
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.001"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            227..1462
                     /gene="arcA"
                     /locus_tag="VH663_000001"
     CDS             227..1462
                     /gene="arcA"
                     /locus_tag="VH663_000001"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEQ8173240.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            1562..1990
                     /locus_tag="VH663_000002"
     CDS             1562..1990
                     /locus_tag="VH663_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8173241.1"
                     /translation="MTDKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQSYGPKCLEDG
                     EYCQSLAKTILKWEKNMDIAMLIAIVGGLLGCYLYLTKNN"
     gene            2030..3043
                     /gene="argF"
                     /locus_tag="VH663_000003"
     CDS             2030..3043
                     /gene="argF"
                     /locus_tag="VH663_000003"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEQ8173242.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMDLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            3213..4706
                     /locus_tag="VH663_000004"
     CDS             3213..4706
                     /locus_tag="VH663_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEQ8173243.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            4723..6054
                     /locus_tag="VH663_000005"
     CDS             4723..6054
                     /locus_tag="VH663_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEQ8173244.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPKGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTEPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGSDKLSLEIGQAYNVVPARASYQG
                     DKLEALQKELDKLGFEYVVKEGELTVYGLAQHAKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAAQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            6074..7024
                     /gene="arcC"
                     /locus_tag="VH663_000006"
     CDS             6074..7024
                     /gene="arcC"
                     /locus_tag="VH663_000006"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEQ8173245.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALISTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKTLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYVNFNKPDQAKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
     gene            7337..8329
                     /locus_tag="VH663_000007"
     CDS             7337..8329
                     /locus_tag="VH663_000007"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEQ8173246.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            8452..8991
                     /gene="rsmD"
                     /locus_tag="VH663_000008"
     CDS             8452..8991
                     /gene="rsmD"
                     /locus_tag="VH663_000008"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEQ8173247.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            8981..9472
                     /gene="coaD"
                     /locus_tag="VH663_000009"
     CDS             8981..9472
                     /gene="coaD"
                     /locus_tag="VH663_000009"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEQ8173248.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            9459..10496
                     /locus_tag="VH663_000010"
     CDS             9459..10496
                     /locus_tag="VH663_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEQ8173249.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDADFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            10918..11895
                     /locus_tag="VH663_000011"
     CDS             10918..11895
                     /locus_tag="VH663_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ8173250.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDRVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTTYVCDSQNFDFESIQ
                     LEASSNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERETIRKR"
     gene            12319..12963
                     /locus_tag="VH663_000012"
     CDS             12319..12963
                     /locus_tag="VH663_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEQ8173251.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPK
                     SKSKRVSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            12967..14046
                     /gene="rlmN"
                     /locus_tag="VH663_000013"
     CDS             12967..14046
                     /gene="rlmN"
                     /locus_tag="VH663_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEQ8173252.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILDDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(14128..14754)
                     /locus_tag="VH663_000014"
     CDS             complement(14128..14754)
                     /locus_tag="VH663_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEQ8173253.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQLQNHFITSQKIFFSLEISLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            14982..15509
                     /locus_tag="VH663_000015"
     CDS             14982..15509
                     /locus_tag="VH663_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEQ8173254.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            15651..15854
                     /locus_tag="VH663_000016"
     CDS             15651..15854
                     /locus_tag="VH663_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEQ8173255.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            15859..16830
                     /locus_tag="VH663_000017"
     CDS             15859..16830
                     /locus_tag="VH663_000017"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEQ8173256.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            16840..17229
                     /locus_tag="VH663_000018"
     CDS             16840..17229
                     /locus_tag="VH663_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEQ8173257.1"
                     /translation="MSPITLILWLLLVGIVGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRAKHILGARNFSAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            17411..19252
                     /gene="typA"
                     /locus_tag="VH663_000019"
     CDS             17411..19252
                     /gene="typA"
                     /locus_tag="VH663_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEQ8173258.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            19374..19625
                     /locus_tag="VH663_000020"
     CDS             19374..19625
                     /locus_tag="VH663_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEQ8173259.1"
                     /translation="MFYLIMAVLIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQTPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
     gene            19981..21339
                     /locus_tag="VH663_000021"
     CDS             19981..21339
                     /locus_tag="VH663_000021"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEQ8173260.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDEDFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISAILAKTTKATVIPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYSNFEVRGDEFLATFDCLRG
                     DA"
     gene            21339..22421
                     /locus_tag="VH663_000022"
     CDS             21339..22421
                     /locus_tag="VH663_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEQ8173261.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFILAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            22422..23570
                     /locus_tag="VH663_000023"
     CDS             22422..23570
                     /locus_tag="VH663_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEQ8173262.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKTKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLI
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMYDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQAPPSPETVLEQAHG"
     gene            23781..25145
                     /gene="ftsA"
                     /locus_tag="VH663_000024"
     CDS             23781..25145
                     /gene="ftsA"
                     /locus_tag="VH663_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEQ8173263.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            25169..26488
                     /gene="ftsZ"
                     /locus_tag="VH663_000025"
     CDS             25169..26488
                     /gene="ftsZ"
                     /locus_tag="VH663_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEQ8173264.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            26491..27161
                     /locus_tag="VH663_000026"
                     /pseudo
     CDS             26491..27161
                     /locus_tag="VH663_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
     gene            27173..27829
                     /locus_tag="VH663_000027"
     CDS             27173..27829
                     /locus_tag="VH663_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEQ8173265.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMS
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            27834..28088
                     /locus_tag="VH663_000028"
     CDS             27834..28088
                     /locus_tag="VH663_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEQ8173266.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            28088..28879
                     /locus_tag="VH663_000029"
     CDS             28088..28879
                     /locus_tag="VH663_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEQ8173267.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVEDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            28889..29647
                     /locus_tag="VH663_000030"
     CDS             28889..29647
                     /locus_tag="VH663_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEQ8173268.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSMTEPEVNLSETQTFKLNI"
     gene            29920..32721
                     /locus_tag="VH663_000031"
     CDS             29920..32721
                     /locus_tag="VH663_000031"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEQ8173269.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQESN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRNDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMMENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNTKENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEHESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGIAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESGK"
     gene            complement(32986..33288)
                     /locus_tag="VH663_000032"
     CDS             complement(32986..33288)
                     /locus_tag="VH663_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEQ8173270.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(33339..33794)
                     /locus_tag="VH663_000033"
     CDS             complement(33339..33794)
                     /locus_tag="VH663_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ8173271.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLGWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(33922..36204)
                     /locus_tag="VH663_000034"
     CDS             complement(33922..36204)
                     /locus_tag="VH663_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ8173272.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPTNSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            36503..36733
                     /locus_tag="VH663_000035"
     CDS             36503..36733
                     /locus_tag="VH663_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEQ8173273.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            36860..37546
                     /locus_tag="VH663_000036"
     CDS             36860..37546
                     /locus_tag="VH663_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ8173274.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISISVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMITTVMAQLLAVL
                     ERHMAQGGNH"
     gene            37546..38280
                     /locus_tag="VH663_000037"
     CDS             37546..38280
                     /locus_tag="VH663_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173275.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
     gene            38429..39838
                     /locus_tag="VH663_000038"
     CDS             38429..39838
                     /locus_tag="VH663_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEQ8173276.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQNHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKYLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            40016..41710
                     /locus_tag="VH663_000039"
     CDS             40016..41710
                     /locus_tag="VH663_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ8173277.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYEEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            41917..42771
                     /locus_tag="VH663_000040"
     CDS             41917..42771
                     /locus_tag="VH663_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEQ8173278.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKDGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            42924..44264
                     /locus_tag="VH663_000041"
     CDS             42924..44264
                     /locus_tag="VH663_000041"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEQ8173279.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQLLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHALANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            44242..44457
                     /locus_tag="VH663_000042"
     CDS             44242..44457
                     /locus_tag="VH663_000042"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEQ8173280.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            44457..45329
                     /locus_tag="VH663_000043"
     CDS             44457..45329
                     /locus_tag="VH663_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEQ8173281.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNA"
     gene            45322..46149
                     /locus_tag="VH663_000044"
     CDS             45322..46149
                     /locus_tag="VH663_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEQ8173282.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKFKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVFASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQKVVAKAHKEFEKHEKE"
     gene            46136..46606
                     /locus_tag="VH663_000045"
     CDS             46136..46606
                     /locus_tag="VH663_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ8173283.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            46628..48289
                     /gene="recN"
                     /locus_tag="VH663_000046"
     CDS             46628..48289
                     /gene="recN"
                     /locus_tag="VH663_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEQ8173284.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIVKEY
                     QLLTGDELSSGDLEAELKALEKQLVAAASELSVSRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISKKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTSEERVEEIARMLAGADMTEAALTQARELLAKH"
     gene            48497..49444
                     /locus_tag="VH663_000047"
     CDS             48497..49444
                     /locus_tag="VH663_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ8173285.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFNDDSFISQNETDKMFENLTDDR
                     LLNELDELDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPH
                     LRSLSDTALRSGEIRGHLDNKLDTLSVTATKLALTMAQKFDLTTHVYSIGESFSEVLA
                     AHYEDRKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAV
                     AKRKIALPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            49660..50511
                     /locus_tag="VH663_000048"
     CDS             49660..50511
                     /locus_tag="VH663_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ8173286.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            50504..51346
                     /locus_tag="VH663_000049"
     CDS             50504..51346
                     /locus_tag="VH663_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEQ8173287.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLKKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            51324..51911
                     /locus_tag="VH663_000050"
     CDS             51324..51911
                     /locus_tag="VH663_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEQ8173288.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            52010..52285
                     /locus_tag="VH663_000051"
     CDS             52010..52285
                     /locus_tag="VH663_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEQ8173289.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            52619..54481
                     /gene="cadA"
                     /locus_tag="VH663_000052"
     CDS             52619..54481
                     /gene="cadA"
                     /locus_tag="VH663_000052"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEQ8173290.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYTSAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAEAASTHPISKAFLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYTLLDDIKI
                     ESKRAIKSLHDMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(54781..55716)
                     /locus_tag="VH663_000053"
     CDS             complement(54781..55716)
                     /locus_tag="VH663_000053"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEQ8173291.1"
                     /translation="MVSTATQISHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLFFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
     gene            55955..56650
                     /locus_tag="VH663_000054"
     CDS             55955..56650
                     /locus_tag="VH663_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEQ8173292.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
     gene            56757..57278
                     /locus_tag="VH663_000055"
     CDS             56757..57278
                     /locus_tag="VH663_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEQ8173293.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILYKGI"
     gene            57439..57981
                     /locus_tag="VH663_000056"
     CDS             57439..57981
                     /locus_tag="VH663_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173294.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            58105..58884
                     /locus_tag="VH663_000057"
     CDS             58105..58884
                     /locus_tag="VH663_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEQ8173295.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFATNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            59681..60277
                     /gene="recR"
                     /locus_tag="VH663_000058"
     CDS             59681..60277
                     /gene="recR"
                     /locus_tag="VH663_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEQ8173296.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            60378..61424
                     /locus_tag="VH663_000059"
     CDS             60378..61424
                     /locus_tag="VH663_000059"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEQ8173297.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEMFDQRESHLI"
     gene            61615..63006
                     /locus_tag="VH663_000060"
     CDS             61615..63006
                     /locus_tag="VH663_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEQ8173298.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGKPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDICLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKE
                     LARLASQVYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVD
                     RLVTDKNCDSYQP"
     gene            63086..63781
                     /locus_tag="VH663_000061"
     CDS             63086..63781
                     /locus_tag="VH663_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEQ8173299.1"
                     /translation="MDFFAIDPIGLPHISLIFYLLSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLAFAFSKEK"
     gene            64029..65573
                     /locus_tag="VH663_000062"
     CDS             64029..65573
                     /locus_tag="VH663_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEQ8173300.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLTKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTSKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            65880..67499
                     /locus_tag="VH663_000063"
     CDS             65880..67499
                     /locus_tag="VH663_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8173301.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            67626..69626
                     /locus_tag="VH663_000064"
     CDS             67626..69626
                     /locus_tag="VH663_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEQ8173302.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWL
                     IIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNMLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLTVYVVILATLAAIIASQALISGSFTLVSEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLYFRTSAHMEAAYGLAITITMLMTTILLNYYL
                     IKEGVKPFLAHLVMTFFALVEFIFFWASAVKFMHGGYVVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVRFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDKPYTAKYKVDMMGTDYMVRVELYLGFRMPQTVPRYLRTIVHD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVSNVRQLTNVERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(69650..69928)
                     /locus_tag="VH663_000065"
     CDS             complement(69650..69928)
                     /locus_tag="VH663_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEQ8173303.1"
                     /translation="MTTKKAYMYVLECADKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(69918..70772)
                     /locus_tag="VH663_000066"
     CDS             complement(69918..70772)
                     /locus_tag="VH663_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEQ8173304.1"
                     /translation="MKVDFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIVEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGAAS
                     KGKPNHDH"
     gene            70812..71552
                     /locus_tag="VH663_000067"
     CDS             70812..71552
                     /locus_tag="VH663_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEQ8173305.1"
                     /translation="MFYAYLRGLVVFLLWIVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKMAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTMLFSYIASFVWNPDKHRH"
     gene            71752..72414
                     /locus_tag="VH663_000068"
     CDS             71752..72414
                     /locus_tag="VH663_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEQ8173306.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            72395..74638
                     /locus_tag="VH663_000069"
     CDS             72395..74638
                     /locus_tag="VH663_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEQ8173307.1"
                     /translation="MTFSWTKLVPLSKIQFAFLILVFFYQIHSPSWLTFLLSLSLICL
                     LVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLEIQPVQITSVALVPDSIRINGDQ
                     LAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
                     FLAYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLL
                     FGYLDKSFGEMTDYYSQLGIIHLFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELP
                     FASFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLT
                     FSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTGLLSYLF
                     DLFILPLLCLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLIL
                     VISFAIFYDYRQVRQRVITCGLVIALTLLSVKYPLTNEVTFIDIGQGDSILVREWTGK
                     NLLIDVGGRPFFSSKEHWRREYHVANAQKTLIPYLKSRGIHTIDQLLVTHADTDHMGD
                     IEVVAKAIRIKEILTSQGSLSHPIFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPW
                     QLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTS
                     SSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDRQITYYRTDTQGAIRLTGWTS
                     WHLETVR"
     gene            74709..75749
                     /locus_tag="VH663_000070"
     CDS             74709..75749
                     /locus_tag="VH663_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEQ8173308.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSYQ
                     LGLGFESGAFEQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKVDVARDLIHDLRLSGEDDIKLIAIMLGQFRLYLQLAILAKEGKSEQQL
                     VTSLSEILGRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            75846..76451
                     /locus_tag="VH663_000071"
     CDS             75846..76451
                     /locus_tag="VH663_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEQ8173309.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            76618..76851
                     /locus_tag="VH663_000072"
     CDS             76618..76851
                     /locus_tag="VH663_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173310.1"
                     /translation="MLREKKTTIVGIILGMMIVTLLVGCLTGKKKGFPPKAGERQQTA
                     DLKMKSKIRKTVQKPLPEKSKEAKQAVMDADLA"
     gene            77076..77666
                     /locus_tag="VH663_000073"
     CDS             77076..77666
                     /locus_tag="VH663_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEQ8173311.1"
                     /translation="MTERFDITETDYEYDQEHHAYVAQFNGAMSIPDMQEYSLVCSEN
                     TPAYALAERLGGMNKAYQLFDRYGKVSGAITTIDRNGNKITTAYYLQVLDYLWQHQDK
                     YKDILYYIGESFPDLYYKTYLPHVKVYQKPGYVREALNVGAIVCEESPYLIALYSSGL
                     GGATQASEEVNGLGYVQLVQLTYVINEWHRVNHNMI"
     gene            complement(77820..79040)
                     /locus_tag="VH663_000074"
     CDS             complement(77820..79040)
                     /locus_tag="VH663_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ8173312.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQDLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSALIQACYVNLEHIPYSRFNRKIHQLRYLISTQQTEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLIHHY
                     QETRGDNKTSKTQH"
     gene            complement(79047..80075)
                     /gene="queA"
                     /locus_tag="VH663_000075"
     CDS             complement(79047..80075)
                     /gene="queA"
                     /locus_tag="VH663_000075"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEQ8173313.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSG
                     WTNIFIKPGYQFNVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            80277..80981
                     /gene="nagB"
                     /locus_tag="VH663_000076"
     CDS             80277..80981
                     /gene="nagB"
                     /locus_tag="VH663_000076"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEQ8173314.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYDQIIAEHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIEANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            81100..81816
                     /locus_tag="VH663_000077"
     CDS             81100..81816
                     /locus_tag="VH663_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ8173315.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            82112..83074
                     /locus_tag="VH663_000078"
     CDS             82112..83074
                     /locus_tag="VH663_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEQ8173316.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFRCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     CLSFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            83087..84892
                     /gene="pepF"
                     /locus_tag="VH663_000079"
     CDS             83087..84892
                     /gene="pepF"
                     /locus_tag="VH663_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ8173317.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEIDAAKDFS
                     GHLLDSSANLLTITDTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSIRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
BASE COUNT        26584 a        14794 c        17809 g        25990 t
ORIGIN      
        1 aatttataag tataaatttt tacactaaga tgtaaaaaac atacaagtaa acgaatacaa
       61 atctataaaa ataaaaacaa cgctcaaaaa atgtaagcga ttactcaaaa tagctgtgag
      121 aaatacccta tttttatctt ttttaaggag tacactagga atataagcaa gagttaagaa
      181 tcttgatggt gaattcacaa aaaccaatta gaaagaggta attcttatga ctgctcaaac
      241 accaattcat gtttattctg aaattggaaa attaaaaaaa gtgttgctcc acagacccgg
      301 taaagaaatt gaaaatctta tgccagacta cttggaacgc ttgttgtttg atgatattcc
      361 attcttagag gatgcccaaa aagaacatga tgcttttgcc caagctttgc gtgacgaagg
      421 tattgaagtc ttgtaccttg agaccttagc tgcagaatct cttgtcactc cagaaattcg
      481 cgaagctttc atcgatgaat acctaagtga agctaatatc cgtggaagag ctactaaaaa
      541 agccattcgt gaattgttga tggctatcga agacaatcaa gaattgattg aaaaaaccat
      601 ggcaggagtt caaaaatctg aacttccaga gatcccagca tctgaaaaag gcttgactga
      661 cttggttgaa tccaattacc catttgccat cgacccaatg ccaaaccttt atttcacacg
      721 ggacccattc gcaactatcg gtacaggtgt ttctcttaac cacatgttct cagaaacccg
      781 taaccgtgaa accctttatg gtaaatacat tttcactcat cacccaattt atggtggtgg
      841 caaagtgcct atggtttatg accgtaatga aaccactcgc attgaaggtg gggacgaact
      901 tgttctttca aaagatgtgc ttgcggttgg tatttctcaa cgtacagatg ctgcttctat
      961 tgaaaaattg ttggttaaca tctttaaaca aaaccttggc ttcaagaaag tattggcctt
     1021 tgaatttgca aataaccgta aatttatgca cttagacact gtctttacca tggttgacta
     1081 tgacaaattc accattcacc cagaaattga aggcgatctt cgtgtttact ctgtcactta
     1141 cgacaatgaa gaacttcata ttgttgaaga aaaaggtgat ttagcagaac ttcttgctgc
     1201 taaccttggt gttgaaaaag ttgaccttat ccgttgtggt ggtgacaact tagtagcagc
     1261 aggtcgtgaa caatggaacg atggttctaa taccttgacc atcgcaccag gtgtggttgt
     1321 ggtttataac cgtaatacca ttaccaatgc cattcttgaa tctaaaggct tgaaattgat
     1381 caagattcac ggaagtgaat tggttcgcgg tcgtggtgga cctcgttgta tgtcaatgcc
     1441 atttgaacgt gaagatattt aataagctat ggtaaaggtg gttgtaggtc agaagccttt
     1501 ttaaagggca gctagtgttt atcttagctt ctgtgtcttt gtccttcaat gaaattttgt
     1561 catgacagat aaatttgatg ccaatgacga aacaagaacg gtttatgcag tcgtttatga
     1621 caatgaccag cccgtttcaa caggacaatt tttagctgaa acgaaaatag aagcacgatt
     1681 gacacgcatt gtaaccttag cagattattg tggttgcggt tatggtgcca aagtcactga
     1741 agcgctagaa acttatacca gacgagaagg cttttaccaa ctaaccattc acagtgaact
     1801 gacagcacaa accttttatg aaaacctagg ttatcagtcc tatggtccca agtgtttaga
     1861 agatggtgag tattgtcagt cccttgcaaa aaccattctt aaatgggaga agaatatgga
     1921 catagcaatg ctaattgcga ttgttggtgg tctattaggc tgctatctct atctcacaaa
     1981 aaataattaa tcaaaacaga ttacagttaa cactcgaagg agacaataga tgacacaagt
     2041 atttcaagga cgtagcttcc tagcagaaaa agattttaca cgcgctgaat tagaatacct
     2101 tattgatttt tcagcacatt tgaaagattt gaaaaaacgt ggtgtgcctc atcactactt
     2161 agaaggtaaa aacattgccc tcttgtttga aaaaacatca actcgtacgc gtgcagcttt
     2221 tacaacagca gccattgacc taggtgctca cccagaatac ctcggtgcca atgacatcca
     2281 acttggtaaa aaagaatcaa cagaagacac tgctaaagta ttgggtcgta tgtttgatgg
     2341 gattgaattc cgtggcttta gccaacgcat ggttgaagaa ttggcagaat tctcaggtgt
     2401 gccagtttgg aatggtttga ctgatgaatg gcacccaacc caaatgcttg ctgactattt
     2461 aacagtgaaa gaaaactttg gcaaacttga aggccttacc cttgtttact gtggtgatgg
     2521 gcgtaataac gttgccaatt cccttcttgt gactggtgct atccttggtg ttaatgttca
     2581 tatcttctca ccaaaagaac tcttcccaga agaagagatt gtgacattgg ctgaaggcta
     2641 tgctaaagaa agtggcgctc gtatccttat cacagaagat gctgacgaag ctgttaaagg
     2701 cgcagacgtc ctttacacag acgtttgggt atcaatgggt gaagaagaca aattcaaaga
     2761 acgtgttgaa ttgcttcaac catatcaagt caacatggac cttgttcaaa aagcaggaaa
     2821 tgacaagctt atcttcttgc actgcttgcc agctttccat gacacaaaca ccgtttatgg
     2881 taaagacgtt gctgaaaaat tcggcgtgaa agaaatggaa gtcactgatg aagtcttccg
     2941 cagcaaatac gctcgtcatt ttgatcaagc tgaaaaccgt atgcacacta tcaaagcggt
     3001 tatggcagca acccttggta acctctttat tccaaaagtg taataaggac tggtactcct
     3061 taactcattt cacatatcaa tgtgatccct ttaatttaac aatttaacta cttaaaaacg
     3121 tactttaact acatgacaag tcgttctggt tttcgcgtcc ttgaccggaa ctagaacggc
     3181 ttatttttat gaaaaggtaa gaggtaatca ttatgacaga agaaaaaaaa cggggtttta
     3241 gaattccttc ttcttacacc gttcttttta tcattatagc catcatggca gtgttaactt
     3301 ggtttattcc agctggcgcc tacgaaaccg ctaagggcgg tggtgttatt tcaggaacct
     3361 acaaaacagt agcatccaat cctcaaggat tttttgacat cttgatggct cccgttcgag
     3421 gcatgttagg tgttgaaggc actgatggtg ctattcaagt gtctttcttt atcttaatgg
     3481 ttgggggctt tttaggagtt gtcaacaaaa caggtgctct tgacacagga attgcgtcag
     3541 tggttcgtaa aaacaaaggt agagaaaaaa tgctgattgc tattttaatc cctttgtttg
     3601 ctctaggtgg aacgacctat ggtatggggg aagaaaccat ggccttttac ccacttttaa
     3661 ttcctgttat gattgcggtt ggttttgaca gtattgttgc tgtagccatt attttgattg
     3721 gttctcaaat tggatgtcta gcctccacta ttaacccatt tgcaactggt gtagcagctg
     3781 atgctgctgg tgtcagcatt gcagatggaa tgatttggcg tgttatccaa tgggttattc
     3841 ttgtcggcat gtctatttgg tttgtttaca attacgctag taagattgaa gaagacccaa
     3901 gtaaatcatt ggttgcagat aaggaagaag agcataagga actcttccag ttgcaaaatt
     3961 ctggagaaga cttaaacaaa cgccaacgaa acgttttgac catttttact ttgacctttg
     4021 tcattatgat tcttagtttg attccgtggg aagattttgg tatcaaattc tttactaata
     4081 tcaatacttg gttaaccaca atgcctatct tagggggagt tattgggaaa accatgggag
     4141 catttggtac ttggtatttc ccagaaatca ccatgctctt tatcatgatg ggtgtcttag
     4201 ttgctattgt ttatcgtatg agtgaagaag acttttttag ctcttttttg actggtgcag
     4261 gtgaattttt aggtgttgcc atgatctgtg ctattgcacg cggtattcaa gttatcatga
     4321 atggtggtat gattacagcc actatcttac acttaggtga aacaagtctt tctggtttat
     4381 cttctcaagt ttttgtgata ttagcttata ttttctacct cccaatgtcc ttcttgattc
     4441 catcaacatc aggacttgct ggggctacaa tgggaattat ggcaccgctt ggacaattct
     4501 caaatgtccc tgctcacctt gttattacag ccttccagtc agcttctgga atcttaaaca
     4561 tgatttctcc aacttcagca atcgttatgg gagcacttgc gcttggtcgc gttgaccttg
     4621 gtacttggtg gaaattcatt ggtaaattta ttgtaatggt gatgcttgta agcgtgctat
     4681 tacttgtagt tgcaacattc ttttaacaga taggaggttc ctatggaatc ctatatcaca
     4741 cctaaacatc aagaggcttg tgtagctgcc attaaacaga tcgtttctta cccttctgtt
     4801 tgtcatgaag gagaaaatgg cacacctttt ggacaggcca ttcaagatgt tctcgaagca
     4861 accttagact tatgtcaagg tcttggcttc catacttata ttgatcctaa aggttactat
     4921 ggttatgctg agttaggtga ccaaaaagag gtcttggcta ttctgtgcca tttggacgtt
     4981 gttccagaag gagatcgtca gttatggaag acagagcctt ttgactgtgt tgaggcagac
     5041 ggctgtttat ttggtcgagg cactcaagac gataaagggc ctactatgat ggcactattt
     5101 gccaccaaag ccttgataga tgcaggcgtt acctttaata aacgcattcg ttttattttc
     5161 ggaacagatg aagaaacttt gtggcggtgt atgaatcgct acaatgatgt agaagaacag
     5221 gctacctttg ggtttgctcc tgattcaagt ttccctttaa cctatgccga aaaaggccta
     5281 cttcaggcaa aattagtggg gaaaggttct gataaactgt cacttgaaat cggacaagcc
     5341 tacaatgttg ttcctgcaag agcctcttat caaggtgata agcttgaggc tttacaaaaa
     5401 gaactagata agcttggctt tgagtacgtc gtcaaagagg gagaattaac ggtttatggt
     5461 ctcgcccaac atgctaaaga tgctcccgat ggcatcaacg ctatgattag gcttgccaaa
     5521 gctttagtgg tccttgaacc taacaaaacc cttgattttc ttgctaatgt cattgatgaa
     5581 gatggcagag cacttaatat ttttggcaga atcgaagatg aaccttcagg caaattaagc
     5641 tttaatgccg cagggcttac cttaaccaaa gacaaggcag aaattcgcct agatattcgg
     5701 attccagttt tggcagataa ggaaaaatta gtccaacaat taagccaaaa agcacaagaa
     5761 tacggcctaa cctatgaaga atttgattat ttggctccat tatatgtgcc attagacagt
     5821 gaattggtaa caaccttgtt atctgtctat cggaaaaaaa caggtgatca aagccctgcc
     5881 caatcatcag gaggggcaac ctttgctaga acaatgaaga attgtgttgc ctttggtgcc
     5941 cttttccctg atgctgctca aacggaacac caagaaaatg aacatattgt tttagcggat
     6001 gcttaccgcg ctatggatat ttatgcggaa gctatttacc gactaacgcg ataacaagat
     6061 atgaggagta attatgacga aacaaaaaat cgtagtcgca cttggaggaa atgctattct
     6121 ttcaacagat gcgtcagcaa aagcacaaca agaagccttg atttccacat caaaatcatt
     6181 ggttaaatta attaaagaag gtcatgaagt catcgtgacc cacggcaatg gcccacaagt
     6241 tggtaatctc ttattacaac aagcagcggc agattctgaa aagaatccgg ctatgccttt
     6301 ggatacttgt gttgccatga cagaaggtag catcggtttt tggttagtta atgcccttga
     6361 taatgaattg caagcccaag gtattcaaaa agaagtggca gctgttgtca ctcaagtgat
     6421 cgtggatgcc aaagacccag cttttgaaaa cccaacaaaa ccaattggtc catttcttac
     6481 cgaagaagat gccaaaaaac aaatggcgga gtctggtgca agtttcaaag aagatgcagg
     6541 tcgtggctgg cgtaaggttg tgccatcacc aaaaccagtt ggtattaaag aagccaatgt
     6601 catcagaagt ctcgttgatt ctggcgttgt tgtggttagt gctggtggtg gcggtgtgcc
     6661 cgttgtggaa gatgctacta gcaagaccct gactggtgtg gaagctgtta ttgacaagga
     6721 ctttgctagt caaacccttt cagaattggt ggacgcagac ctctttatcg ttttgacagg
     6781 agttgataat gtttacgtca acttcaacaa acctgaccaa gccaaattgg aagaagtgac
     6841 tgtctctcaa atgaaagaat acatcacaca agaccaattt gcaccaggta gtatgttacc
     6901 aaaagtggaa gctgccattg cttttgttga aaacaaacca aatgccaaag ccattatcac
     6961 atcgctagaa aatattgaca atgtactatc agcaaacgct ggaacacaaa ttatcgcagg
     7021 gtaatcaaga ggatttttag ttctgtttaa aagactcatt taatggtatt ttctgaagag
     7081 tacgactgac ataaagagtt gatactgttt ttaaaggaaa agagtgtgcc ataataaaaa
     7141 acaataatag tattagaaaa ggctaatttt attgagaatt agccttttct tttagctttg
     7201 ataggtgtat aataaccata ctatttttta tttttcaaaa tattagaata aacaaaagtg
     7261 gacaacgtat aaagagaact gctaagtcgt tgagctcatt agtcccaaaa agattaccaa
     7321 aaaagaagga agaactatga agaaaagttt tattcatcaa caagaggaga tttcctttgt
     7381 taagaatacg ttcactcagt atttgattgc taagttagat gttgttgaag tgcaaggacc
     7441 cattttaagt cgggtcgggg acggtatgca agataatttg tccggtactg aaaatccagt
     7501 atctgtcaat gtcttgaaaa ttcctaatgc aacctttgaa gttgtgcatt cattagcaaa
     7561 atggaaacgt cacaccttag cccgttttgg gtttaatgaa ggtgaaggcc ttgttgttaa
     7621 tatgaaggct ctccgacctg atgaggattc acttgaccaa acccattctg tctatgttga
     7681 ccaatgggac tgggaaaaag tcatccctga tggcaaacgt aatctcgctt acctcaaaga
     7741 aaccgttgaa accatttaca aggtcattcg gctaactgaa ttagctgtag aagctcgtta
     7801 tgatattgag gctgttttac ctaagaaaat aaccttcatt cataccgaag aattggtagc
     7861 taaataccct gatttgacgc ccaaagaacg tgaaaatgcc attactaaag aatttggagc
     7921 agtcttttta attggtattg gcggggtttt acctgatggt aaaccacacg atggccgcgc
     7981 tcctgactat gatgactgga caacagaaac tgaaaatggc tatcatgggc ttaatggtga
     8041 tattttggtt tggaatgacc aacttggttc agcctttgaa ttatcctcaa tggggatccg
     8101 tgtggatgaa gaggccctaa aacgtcaggt ggaaatgact ggtgaccaag accgcttagc
     8161 ctttgattgg cacaaatctt tgttgaatgg acttttccct ctaacgattg gtggaggaat
     8221 cggtcaatca cgtatggtca tgtttttgtt gcgtaaaaaa catattggag aagttcagac
     8281 ctccgtttgg ccccaagaag ttcgtgatag ctacgataat attttataag taatcacatc
     8341 attttgctaa gttttccact tgtataagag actttaaata aatgatcaga aatattgaaa
     8401 aaagaaatca tttttaaggt ttagagctag aaatttgcta taataaatgt tatgagagtt
     8461 gtatcaggtg aatttggtgg gcgtccttta aagacgctcg atggaaagat aacacgccct
     8521 acttcagaca aagttagagg tgcgattttt aatatgatag gaccttactt taatggtggt
     8581 cgtgttttag atttgtttgc aggttcaggt ggattagcca ttgaggctgt ttctcgtggg
     8641 atgtctgctg cagttttggt tgagaagaat cgtaaagcgc aagctattat tcaagacaat
     8701 attataatga ctaaagctga gaatcggttt accttgttaa aaatggaagc tgagcgagct
     8761 attgattgct tgactggtcg atttgactta gtctttttag accctcctta tgccaaagaa
     8821 acaattgtgg caactattga agctttagca gctaaaaact tattgagtga acaggttatg
     8881 gttgtctgtg agacagataa aacagtcttg ttgccaaaag agattgcaac gttaggtatt
     8941 tggaaagaaa aaatttatgg aattagtaag gtaacagttt atgttaacta aaataggact
     9001 ttatacaggg tcatttgatc ctgttacaaa cggtcatctc gatattgtta aacgggcaag
     9061 tggcctattt gatcaaatat atgttgggat ttttgataat cctaccaaaa aaagctattt
     9121 taaactagaa gttcgtaaag caatgcttac tcaagctctg gctgatttta caaatgtaat
     9181 tgtagtaact tcgcacgaac gtttagctat tgatgtagct aaagaattac gagtaactca
     9241 cttgattaga ggcttgcgaa atgcaactga ctttgagtat gaagaaaatt tggaatattt
     9301 taatcacctt ttagctccaa atattgagac cgtctatttg atatcaagaa ataaatggca
     9361 ggcccttagc tctagtcgtg tgagagagct aattcatttt cagtcttcct tagaaggttt
     9421 ggttcctcag tcagttattg ctcaagtgga gaaaatgaat gaaaagactt aaaaaaatca
     9481 aatggtggtt agtgggtctg ctagctttaa tctctttatt gctagcgtta ttttttccgc
     9541 taccttatta tattgaaatg cctggaggcg cttacgatat tcggactgtc ttacaagtca
     9601 atggcaaaga agacaaacga aaaggagctt accagtttgt tgcagtgggc attagtcgtg
     9661 ccagcctcgc gcagctatta tatgcttggc tgacaccgtt tactgaaatt agtacagcag
     9721 aagatacaac aggcggatac agcgatgctg atttccttcg aattaatcaa ttctacatgg
     9781 aaacatcaca aaatgcagct atttatcaag ctttatcctt agctggaaaa ccagttacat
     9841 tagattataa aggcgtatat gttttagacg taaacaacga atctactttt aaaggaacgt
     9901 tacacttagc agatactgta acaggtgtaa atggtaaaca gtttactagt tcagcagaac
     9961 ttattgacta tgtttctcac ctaaaactag gggatgaagt tacggttcag tttacgagtg
    10021 ataataagcc taaaaaagga gttggccgta ttatcaaact gaaaaatggg aaaaatggga
    10081 ttggcattgc cttgactgat catacaagtg tcaattcaga agacacagtg atctttagta
    10141 ctaaaggagt aggaggacct agtgctggtc taatgtttac tcttgatata tatgatcaaa
    10201 taactaaaga agatttacgc aagggccgta caattgcagg tacaggaact attggcaagg
    10261 atggcgaagt aggagatatt ggtggtgcag gtcttaaagt agttgcagca gctgaagctg
    10321 gtgcagatat attttttgtt ccgaataatc ctgttgataa ggaaattaaa aaagttaatc
    10381 caaatgctat aagtaattac gaagaagcca aacgggcagc caaacgacta aagaccaaaa
    10441 tgaagattgt tcctgttacg actgttcaag aggcactggt ttatcttcgc aaataaaata
    10501 actatgaatg tattatattc aattatattc aattatattc acgctattaa ctcataaaaa
    10561 aacggttgaa ccagatagac agatccttga ccagtctttt gggttcaatc attttttaca
    10621 taaaacataa ctaacttgac agcgtaaaaa agatgttata aaagtctagt ggtaaaaagg
    10681 caatagagaa ttgagtaaac tttagtcgat tatctggaaa tagagcatac taaaaacacc
    10741 tagtgaaaat tgattttttt cactaactca tccaagaact tgcgcagata atcatgagtc
    10801 cattccatgt ctctattatt caaagccttg caatagacaa gttaacgtta taacaactaa
    10861 attaaaaact tgagattgaa aaatccccat aaacttattt tacaaggagg taaggctatg
    10921 gtaaccatta aacaagttgc tgaagaagca ggtgtttcta gatcaacagt ctccaggtat
    10981 atttctcaaa aaggatatgt atctgatgat gcaaggcata aaatcaaagc agcgatagca
    11041 aaattacatt acactccaaa tgtcttggcg cagtcattga agacgaaaaa aaatcaacta
    11101 gtcggtttat tattaccaga catttccaat cctttttttc cgaggttagc aaggggagca
    11161 gaagaatatt taaaagaaaa ggggtatcgg gttatgctag gaaatatttc agacagcgaa
    11221 gctcttgaag aagaatacgt ccatgtcctt cttcaaagca atgctgctgg cattattact
    11281 acccatgact ttacaaagcg gtatcctact cttgctattc cagttgtagt ggtagatcgt
    11341 gttgaccaag agacccagta cggtgtcttt tcagataata gggcaggggg cttattggcg
    11401 gctcagactg tctggcaagc aggggcaaaa gaagttttgt taataagagg tccgctggat
    11461 aatgctgaaa acattaatga gcggtttgaa gctagctttt cttaccttca aaaacaagac
    11521 gtgactacgt atgtgtgtga tagtcagaat tttgattttg aaagtattca gctagaagct
    11581 agttccaacc tcaagtgtta cccaacaata gacagtatta tagcaccttc tgatatccat
    11641 gctattgctt atattcatga actgcattct caaggtaaaa agattcctca agatgttcaa
    11701 ataataggtt atgatgacat tttaatgagt caatttattt atccttcttt atcgactatt
    11761 catcaatcct cttatttgat gggccgttat gccgccgaat tagtttatac tattgctagt
    11821 caattaactg tgaaagcaaa tcggattaaa ttgcctgttc actacgttga gcgagaaaca
    11881 ataaggaaaa gataagtaat aatattagtc cagattttag caggtatgat gaagatttat
    11941 tgggatttac cttctacttt aaaagatgtt atctgtcatt actttcaccg ctatcagtca
    12001 aaacgcattc ttatgcaatt cttaacctta agcttaaggt ctttttgata acatagagga
    12061 atgttacacg acgatgacgc aataaagcct aaatatagta attgtcgtga ttttatagct
    12121 agtgagtaat gagatagcaa gtctctcatt tacttacaaa agtcaaaaaa tagtgtataa
    12181 tggttgagtt acagataaca ctgcaaaaaa gataacaagt gtccataatt atctaagact
    12241 ataaaggcga tatagacaga tataaaaaag atgacgaatc agttatcact ggacaattaa
    12301 ccgatttagt tacaactaat ggatttttca gaaaggtgtc aaatgaaaaa agaaatttcg
    12361 ccagaaatgt acaactataa taaatttcca ggtcccaaat ttattcattt tgaagagcaa
    12421 gttaaggctg aaggcattga tttgttactc ttagaagatg ttaagaacgc ttttgatacg
    12481 actagctttg gtcaacgtta tacagaagtt ctcctaaagt atgactatat tgttggcgat
    12541 tggggaaatg aacagcttcg tcttaaaggg ttttataagg atagtgacga tatcaagaaa
    12601 acgaatcgca tctcacgttt agaagattat attaaagaat tttgcaattt tggttgtgct
    12661 tattttgttt tagaaaatct tcatccacaa gatattaaat ttgaagagga gcgccaacca
    12721 agacgaaaga aatcacctaa atcaaaatca aatcgtcgca agccaaacta ttcaaatcag
    12781 cagcccgcaa cacctaagag taaatcgaag cgggtatcaa aagaaaagca acctgaaaac
    12841 caagcgttta ccagtcaaaa acgtcgaagt aatactaagc ataaagaaaa gtcaaaacgt
    12901 aatcagacta gtcaacttaa taccaaaatt agtcatttta tcatcagaaa gaaagataaa
    12961 taatccatga aaccatcgat ttatagctta acacgcgatg aactgatcgc gtgggcagtt
    13021 gaacgcggcc aaaaacagtt tagagctact caaatttggg attggctcta taaaaaaaga
    13081 gttcaatctt ttgaggagat gacgaatatt tctaaagatt ttgtttctat tttagatgat
    13141 agtttctgtg tcaatccttt aaaacagcga gttgtacaag aatcagctga tggtacagta
    13201 aaatatttat ttgaactgcc cgatggtatg ttaattgaaa cagttttaat gcgtcaacat
    13261 tatggtcatt ctgtttgtgt gacgactcaa gttggctgta acattggctg tactttttgt
    13321 gctagtggat tgatcaaaaa gcaacgtgat ctaaacagtg gggaaattac agctcaaatc
    13381 atgttagtcc aaaaatattt tgacgataga aagcaaggtg agcgggttag ccatgttgtc
    13441 gtaatgggca ttggtgaacc ttttgataac tacaaaaatg tcatgtgctt tcttcgcgtc
    13501 atcaacgatg ataatggctt agcaattggt gcccgtcata ttacagtctc tacctcaggg
    13561 ttagctcaca agattcggga ttttgcaaac gagggagtac aggttaatct agcagtctcg
    13621 ttgcatgcac caaataatga tttacgttca agtattatgc gagtaaatcg ctcttttccg
    13681 ctggaaaagc tcttttcagc tatagaatac tatattgaaa aaacgaatcg tcgtgtcact
    13741 tttgaataca ttatgctaaa tgaagttaac gatagcatta agcaagctca agaattagca
    13801 gacttaacta aaactattcg taagttatct tatgttaact tgattccgta taatcctgtt
    13861 tcagaacatg atcagtatag tagaagtcct aaagagcgag ttctagcgtt ttatgatgtg
    13921 ctaaagaaaa atggagttaa ctgtgtggtg cgtcaagagc acggaacaga tattgatgca
    13981 gcctgtggcc agttacgttc taaaaccatg aaaaaagatc gcgaaaaggt tacagcgaca
    14041 aaataaagcc caaaaaatat ctttgattga caatgcttga tgacaaaata ttcctagtca
    14101 ctaaaatgac taggtttttt atctggccta aaaatgctct agaagcacaa tccaaaataa
    14161 tccaaaacta agataaggaa taaaaggtat gcgcttgatg cctaatagta agcaagcagt
    14221 aatacctgct aaactagcaa gttgaattag ccaaatcaac gacgttagat ccaatactaa
    14281 agctaagcta gcaaggtaga aaaaatcgcc tgctccaatt gaaaaaggtc ttaaatgagc
    14341 taataaacct agtagcagta atatcaatga taaataattg atcgaataaa acaggagtag
    14401 acttacaaag ctaaaaagcc acaagataaa aggatattct tgagaacgcc aatcaaataa
    14461 actaaataat aaagaaaaga gtaacaagta acagtaacta gcactaaccc aatgtaccat
    14521 actagcccat aagataatta ttagacttat ctctaagcta aagaagattt tttggctagt
    14581 tataaaatgg ttttggagct gccgaaaaac gcgtttggca aaagttagtt ggtcacaaac
    14641 aatgacagtt tgtttactta gctgttcttg accattatca ggaaaataaa gtagtaaaca
    14701 gtaaaaaaga tgggtcaata agatgcccga acatatacta aaaaaagcta tcatacttat
    14761 ttattcgaaa agaaatcaaa tacttgcaat ctttccttaa tcattgtaga atatagatat
    14821 aaaacgataa tgattctaat taagtatcta atcatccaat taggattatt ccgttattgt
    14881 cgaataggat taggtcctta accctttctt atgtttatca cgttagggtt agttccccct
    14941 ccattcttgc cctttaagct gaaaagtaag gagatgttat tatgacaaac acactcgttg
    15001 aaaatattta tgcttcagta actcacaata tttctaaaaa agaagcatca aaaaatgaaa
    15061 aaactaaagc tgttttaaac caagctgtcg ctgacttatc tgtggctgca tctattgtgc
    15121 accaagtaca ctggtatatg cgtggtccag gtttcttgta ccttcaccca aaaatggatg
    15181 aattacttga tagcttgaat gctaatttag atgaaatgag cgagcgtttg atcactattg
    15241 gcggagcgcc ttattctacc ctagctgagt tttctaaaca ctctaaattg gacgaagcaa
    15301 aaggtactta cgataaaaca gttgctcagc atttagcaag attggttgaa gtttaccttt
    15361 acctcagcag cctttaccaa gttggtttgg atattaccga tgaagaaggc gatgctggaa
    15421 caaacgattt gtttacagca gctaagacag aggctgaaaa aaccatttgg atgctccaag
    15481 cagagcgtgg acaaggccca gcactctaat ggattatgcc ttaggataaa agaagtcaat
    15541 gataaaattg gcttcttttt tatggaatca tcgattcatt ttcagaaaca tagaaagcga
    15601 ttccataatt gtcttgaaat ctgccgtaga atttgctaaa atctacttat atgaaaacac
    15661 tatatgatgt gcaacaattg ctaaaaaact ttggcatttt tgtttacctt ggcaaacgtc
    15721 tctatgatat tgaaatgatg aagattgaac ttcagcgtct ctatgatagc ggcttattgg
    15781 ataagaggga ttacttaaat gcggagttaa ttttgcgacg tgagcataga ttagaattgg
    15841 aaaaagaagg atagacaaat gagtcaaaaa ttactgggga ttgatttagg tggtaccacc
    15901 attaaatttg gaattttaac agcagcagga gaagtccaag aaaaatgggc aattgagact
    15961 aatattttag aaggaggcaa acatattgtc cctgatatca tcgcttccat caaacatcgc
    16021 ttagacttgt atggtctaag tagtgctgat tttgtgggaa ttggtatggg atcaccagga
    16081 gcggttgacc gtgatactaa tacagttact ggggcattca accttaattg gaaagaaacc
    16141 caagaagtcg gttccgttgt tgaaaaagaa ttgggcattc cttttgccat tgacaatgat
    16201 gccaatgtgg ctgcccttgg tgaacgttgg gtaggtgctg gtgaaaataa cccagatgtc
    16261 gtcttcatga cacttggaac aggtgttggt ggaggcatta ttgctgatgg taacttgatt
    16321 catggtgttg caggagcagg tggtgaaatc ggccacatga ttgttgagcc agaaaatggc
    16381 tttgcttgta cttgtggctc acacggctgt ttggaaacag tagcttcagc aacaggagtt
    16441 gtcaaagtgg cacgtttact ggcagaagcc tacgaagggg attcagccat caaagcagct
    16501 attgacaatg gtgaaggtgt taccagtaaa gacattttca tggcggctga agcaggggat
    16561 tcctttgctg attctgttgt ggaaaaggtt ggttactacc ttggccttgc ttcagcaaat
    16621 atttccaata tcttgaatcc agactcagtg gttatcggtg ggggtgtgtc agcagcagga
    16681 gaatttctac gctcacgtat tgaaaaatac tttgtgacct tcactttccc acaagtgagg
    16741 tattcaacta aaattaagat tgcagagctt ggcaatgatg ctggaattat cggtgcagct
    16801 agccttgctc gacaatttat cacaaaatag gagggggata tgtctccaat cacacttatc
    16861 ttatggttac ttcttgtcgg cattgttggt tattatacat ggaactattt ctcttttcga
    16921 aaaatggcaa agcaggttga taacgagact tttaaagacg tgatgcgtca aggtcaattg
    16981 attgatttac gagagccagc ggcttttaga gctaagcata ttttaggagc tcgaaatttt
    17041 tcagcgcaac aatttgatgc tgctatcaaa ggtcttcgca aggacaaacc tgttcttatt
    17101 tacgaaaata tgcgtcccca gtaccgtgtg cctgcggtca aaaaacttaa aaaagctggc
    17161 tttgaagacg tttatgtctt aaaagacggt attgattatt gggatggcaa agttaaacaa
    17221 acaacctagt taagagtaac gtggagttaa ggggaataaa ggcagtcact gtctcaaaaa
    17281 ccttaattcc ttttttgctg tatccagact tgctgaaagt ctgaaaacat ttacaattga
    17341 ttaaaaccag ttttttaaaa cattttgtgt tatacttatc tagttaaaat atatttactt
    17401 agaggaacaa atgactaact taagaaacga tatccgtaac gtagcgatta ttgcccacgt
    17461 tgaccacgga aaaacaacac ttgtagatga attattaaaa caatcccata ctcttgatga
    17521 gcgtaaagag cttcaagagc gtgccatgga ttccaatgac cttgaaaaag aacgtgggat
    17581 tacaatcctt gcgaaaaata cggcagtagc ctataacgat gttcgtatta acatcatgga
    17641 taccccagga cacgcggact tcggtggtga agttgaacgt atcatgaaaa tggttgacgg
    17701 ggttgttctt gttgtggatg cctacgaagg aacaatgccc caaacgcgtt tcgtattgaa
    17761 aaaagcactt gagcaaaacc ttatcccgat cgttgtggtg aacaagattg acaaaccttc
    17821 agctcgtcca gcagaagttg tagatgaagt gcttgaatta ttcatcgaac ttggtgccga
    17881 tgatgagcaa ttggaattcc cagttgttta cgcatcagct attaatggca catcatcatt
    17941 atcagatgat cctgctgacc aagagcatac catggcacct atctttgata cgattattga
    18001 tcatattcca gcgccagttg ataattcaga tgagcctttg caattccaag tgtcactttt
    18061 ggattacaac gatttcgtag gtcgtatcgg tatcggtcgt gtcttccgtg gtactgttaa
    18121 agttggtgac caagtaactc tttcaaaact tgatggtacc actaaaaact tccgtgttac
    18181 aaaactgttt ggtttcttcg gtttggaacg tcgtgaaatt caagaagcta aagcaggtga
    18241 cttgattgct gtttcaggta tggaagatat ctttgttgga gaaaccatta caccaactga
    18301 ttgtgtggaa gctctgccaa ttcttcgtat tgatgagcca acgcttcaga tgactttctt
    18361 ggtcaataac tctccttttg caggtcgtga aggtaaatgg atcacgtcac gtaaggttga
    18421 agaacgtctt ttagcagaat tgcaaacaga cgtgtcactt cgtgttgacc caacagattc
    18481 gccagataag tggacggttt cagggcgtgg agaattgcac ttgtcaattc ttatcgaaac
    18541 catgcgccgt gaaggctatg aacttcaagt atcacgtcca gaagttatca tcaaagaaat
    18601 tgatggtgtc aaatgtgaac cgtttgagcg tgttcaaatt gatacaccag aagaatatca
    18661 gggtgcaatc atccagtctc tttcagaacg taaaggggat atgcttgata tgcagatggt
    18721 tggtaatggt caaacgcgtt tgattttctt gattcctgca cgtggtttga ttggttattc
    18781 aacagagttt ctttcaatga cacgtggata tggtatcatg aatcatactt ttgatcagta
    18841 tctaccggtt gttcaaggag aaattggtgg tcgtcatcgt ggtgccttgg tttctattga
    18901 aaatgggaaa gcaacgacat attcaatcat gcgtattgaa gaacgtggaa ctatctttgt
    18961 caacccaggt acagaagttt acgaagggat gattgttggt gaaaactcac gtgataacga
    19021 ccttggtgtt aacattacaa cagctaagca aatgacaaat gtccgttcag cgactaaaga
    19081 tcaaactgca gttatcaaga cgcctcgtat cctaacgctt gaagaatcat tagagttctt
    19141 gaatgacgac gaatacatgg aagtaacgcc agaatctatc cgcttgcgta aacaaatctt
    19201 gaataaagct gcgcgtgata aagctaataa aaagaaaaaa tcagccgaat agcttttgaa
    19261 gttaaactaa ctaaataaaa tagcaaaaag atatttaaga aaagtcattt tttaaaggtc
    19321 tttttgcgtt tacaagttcc tatggaatat aaaccaaaat aaaggaggac ggaatgtttt
    19381 accttattat ggctgtcttg attatatctt attatctata catggctcct aaaagtgtcc
    19441 gtaacaccct tgggatgatt ggtttagttg gcttggttgc tctgcttatt gttttagcgg
    19501 gcttgagttt tatcaaaatc atgcaaaccc ctcctgaatt ttttattggg atgggaatgg
    19561 tggcattggg ctattttgct ttaaaagatg tgcgtaaaat gaccaaaaaa ccgagagtta
    19621 aataaaagta cttaaagatt gatgattttg gtttttatac gataaaagga ctgatttgtt
    19681 ttagacaata tcagtccttt tttaatagtt cattttttct gaaagaaagc ttttagccga
    19741 ttttcattga gtgtgcaact aaaaagagat gcttggtagt gacagttaag acctctgact
    19801 agtattttat cttattttac ttaaaaaaac gtaccaataa tctcacaaat atgggcatga
    19861 tgtttgtata ttggaaacgg attctaattc taaactcaag aacggtattt ttaaaatgtt
    19921 gaaaaagcat ctaaataaag gtaaaataga aatcaaatag aatggatagt gtgagcttag
    19981 atgaaagtga taagtaattt tcaaaacaaa aaaatattaa tattggggtt agccaaatca
    20041 ggcgaagcag cagcaaaatt attgactaaa cttggtgctt tagtgactgt taatgatagt
    20101 aaaccatttg accaaaatcc agcggcacaa gccttgttgg aagaggggat taaggtcatt
    20161 tgtggtagcc acccagtaga attattagat gaggactttg agtacatggt taaaaaccct
    20221 gggattcctt atgataatcc tatggttaaa cgcgcccttg caaaggaaat tcccatcttg
    20281 actgaagtag aattggctta tttcgtatct gaagccccta ttatcgggat tacaggatca
    20341 aacgggaaga caaccacaac gacaatgatt gccgatgttt tgaatgctgg cgggcaatct
    20401 gcactcttat ctggaaacat tggttatcct gcttcaaaag ttgttcaaaa agcaattgct
    20461 ggtgatactt tggtgatgga attgtcctct tttcaattag tgggagtgaa tgcttttcgc
    20521 cctcatattg ctgtcatcac taatttaatg ccgactcatc tggactatca tggcagtttt
    20581 gaggattatg ttgctgctaa atggatgatt caagctcaga tgacagaatc agactacctt
    20641 attttaaatg ctaatcaaga gatttcagca attctagcta agaccaccaa agcaacagtg
    20701 attccttttt caactcaaaa agtggttgat ggagcttatc tgaaggatgg aatactctat
    20761 tttaaagaac aagcgattat agctgcaact gacttaggtg tcccaggtag ccacaacatt
    20821 gaaaatgccc tagcaactat tgcagttgcc aagttatctg gtattgctga tgatattatt
    20881 gcccagtgcc tttcacattt tggaggcgtt aaacatcgtt tgcaacgggt tggtcaaatt
    20941 aaagatatta ccttctacaa tgacagtaag tcaaccaata ttttagccac tcaaaaagct
    21001 ttatcaggtt ttgataacag tcgcttgatt ttgattgctg gcggtctaga tcgtggcaat
    21061 gaatttgacg atttggtgcc agacctttta ggacttaagc agatgattat tttgggagaa
    21121 tccgcagagc gtatgaagcg agctgctaac aaagcagagg tctcttatct tgaagctaga
    21181 aatgtggcag aagcaacaga gcttgctttt aagctggccc aaacaggcga tactatcttg
    21241 cttagcccag ccaatgctag ctgggatatg tattctaatt ttgaggttcg tggggatgaa
    21301 tttttggcaa cctttgattg tttaagagga gatgcctaat gcctaagaag attttattta
    21361 caggtggtgg aactgtaggt catgtcacct tgaacctcat tctcatacca aaatttatca
    21421 aggacggttg ggaagtacat tatattggtg ataaaaatgg cattgaacat acagaaattg
    21481 aaaagtcagg ccttgacgtg acctttcatg ctatcgcgac aggcaagctt agacgctatt
    21541 tttcatggca aaatctagct gatgttttta aggttgcact tggcctccta cagtctctct
    21601 ttattcttgc caagcttcgc cctcaagccc ttttttccaa aggtggtttt gtctcagtac
    21661 cgccagttgt ggctgctaaa ttgcttggta aaccagtctt tattcatgaa tcagatcggt
    21721 caatgggact agcaaacaag attgcctaca aatttgcaac taccatgtat accacttttg
    21781 agcaggaaga ccagttgtct aaagttaaac accttggagc ggtgacaaag gttttcaaag
    21841 atgccaacca aatgcctgaa tcaactcagt tagaggcggt gaaagagtat tttagtagag
    21901 acctaaaaac cctcttgttt attggtggtt cggcaggggc gcatgtgttt aatcagttta
    21961 ttagtgatca tccagaattg aagcaacgtt ataatatcat caatattaca ggagaccctc
    22021 accttaatga attgagttct catctgtatc gagtagatta tgttaccgat ctctaccaac
    22081 ctttgatggc gatggctgac cttgtagtga caagaggggg ctctaataca ctttttgagc
    22141 tactggcaat ggctaagcta cacctcatcg ttcctcttgg taaagaagct agccgtggcg
    22201 atcagttaga aaatgccact tattttgaga agaggggcta cgctaaacaa ttacaggaac
    22261 ctgatttaac tttgcataat tttgatcagg caatggctga tttgtttgaa catcaggctg
    22321 attatgaggc tactatgttg gcaactaagg agattcagtc accggacttc ttttatgacc
    22381 ttttgagagc tgatattagc tccgcgatta aggagaagta aatggcaaaa gataaagaga
    22441 aacaaagtga tgacaagctc gttttgacag agtggcaaaa gcgtaacatt gaatttttaa
    22501 agaaaaagaa gcagcaagct gaggaagaaa aaaaactcaa agagaaatta ttgagtgata
    22561 aaaaagcgca gcagcaagct caaaatgctt ctgaagcagt tgagcttaaa actgatgaga
    22621 aaactgatag tcaggaaatt gagtcagaaa cgacgtcaaa acctaaaaaa accaaaaaag
    22681 ttagacaacc caaggaaaaa agcgcgacac aaatcgcttt tcaaaaatcc ttgcctgttc
    22741 ttttgggggc gctcttactc atggcggtgt ctatttttat gatcactcct tatagcaaaa
    22801 agaaagagtt ttctgtaaga ggaaaccatc aaacgaacct tgacgaatta atcaaagcta
    22861 gcaaagtcaa agcatctgac tattggttaa cgttgttaat ttcgcctggt cagtatgaac
    22921 gaccgattct tcgtactatt ccatgggtga aatctgtaca tctctcttac caatttccta
    22981 atcactttct atttaacgtt attgaatttg aaatcatcgc ttatgcacaa gtcgaaaacg
    23041 gttttcagcc tattttggag aatggaaaac gtgtggacaa ggtcagggca tcagaactac
    23101 cgaaatcttt cttgattctt aatttaaaag atgagaaagc gatccaacag ttagttaagc
    23161 aattaacgac attacctaaa aaattagtca agaatatcaa gtcagtgtct cttgcaaatt
    23221 ccaaaacgac agcggattta ctacttattg aaatgtatga cggtaatgta gttagagtac
    23281 cgcagtcaca actcacattg aaacttccct attatcaaaa attgaaaaaa aaccttgaaa
    23341 atgatagtat agtggatatg gaagttggaa tttatactac aacacaggag attgaaaatc
    23401 aacctgaagt tcctcttacg ccagaacaaa acgcagctga taaagaagga gataagcctg
    23461 gtgaacatca ggaacagaca gacaatgatt cagaaacgcc agcaaatcag agtagtcctc
    23521 agcaagcacc accatcccca gaaacggtcc tcgaacaggc ccatggctag ctaatatcta
    23581 agttgaaaaa gcaatgaaaa cgttagaaat tcaacgattc taacccataa tgaattgcct
    23641 aaaaaaatta agtttatata acaaaaaacg taaaatgata acattttacg tttttttatg
    23701 gtataatatt ttctgaatga ttctgttttt tagcagtttt tagaatagca aaagtttgga
    23761 aagtaagtga ggtcaagtga atggctagaa atggcttttt tactggtttg gacattggaa
    23821 caagctcgat aaaagtttta gtagcagaat ttatttctgg tgagatgaac gtcattggtg
    23881 ttagtaatgt tccaagtacc ggcgtaaaag atggcataat aatcgatata gaggcagctg
    23941 cgactgccat caaaactgcg gtagaacaag cagaagaaaa agcagggatg acaattgaaa
    24001 aggttaatgt tgggctaccg gcaaaccttc ttcaaattga accaacacaa ggaatgattc
    24061 ctgtcccaag tgagtctaaa gagataaaag atgaggatgt tgatagcgtt gttaaatcgg
    24121 ctttaacaaa aagtatcaca ccagaacgag aggttatctc tttagttcca gaagagttca
    24181 ttgtggatgg ctttcagggc attcgagatc cacgtggtat gatggggatt aggttagaga
    24241 tgcgcgggct tatttatact ggaccaagca ccattttaca taatctgcgt aaaacggtag
    24301 aaagagcagg cattaaagtt gaaaacatca ttatttctcc gttagctatg gctaaaacca
    24361 ttttaaacga aggtgagcgc gagtttggag ctactgtaat tgatatggga ggtggacaga
    24421 caactgtcgc ttctatgcga gcacaagaat tgcagtatac caatatatat gccgaaggcg
    24481 gcgaatacat tactaaagat atatcaaaag tattaaaaac gtctttggct attgcagaag
    24541 cacttaagtt taattttggt caagcggaga tatcagaagc tagtataact gaaacagtaa
    24601 aagttgatgt ggtaggtagt gaagagcctg ttgaggtaac tgaacgttat ttatctgaaa
    24661 ttatttcagc gcgtattcgt catattttag atcgtgtgaa gcaagatttg gaaagaggtc
    24721 gtttactaga cttaccagga ggcattgttt tgattggtgg cggtgcaatc atgcctggag
    24781 tggtagaaat tgcacaagaa atctttggag taactgtaaa gctccatgtt ccaaatcaag
    24841 tcggtattag aaatccaatg ttttcaaacg ttatcagttt ggtagaatat gttggtatga
    24901 tgtctgaagt agacgtttta gcacaaactg cagtttcagg agaagaactt ttgcgacgca
    24961 agcctatcga tttcagtggt caagaatctt atttaccaga ttatgatgat tcaagaagac
    25021 cagaatcgac cattggctat gaacaacaag cgtcacaaac agcatatgat tcacaagttc
    25081 cgagtgatcc taaacaaaaa atttcagaac gtgttcgtgg catatttggg agtatgtttg
    25141 attaaaagta ataaagtgag gaaataaaat ggcattttca tttgatactg catcaattca
    25201 aggtgcaatt ataaaagtaa ttggagtcgg cggaggtggc ggaaatgcca ttaatcgtat
    25261 gattgatgaa ggtgttgctg gtgttgagtt catcgcagca aatacagaca ttcaggcatt
    25321 aagctcatca aaagctgaaa cggttattca actaggccct aaattaactc gtggacttgg
    25381 tgctggagga caacctgaag taggacgtaa agctgctgaa gaaagcgaag aaattttaac
    25441 agaagctctt acaggagcgg acatggtatt tattactgcc ggtatgggtg gtggctctgg
    25501 gacaggggct gcaccggtta ttgctcgtat cgctaaaagt ttgggagcct tgacagtagc
    25561 tgttgttact cgcccgtttg gttttgaagg taacaaacgt ggtaattttg ctattgaagg
    25621 tatcgaagaa ctacgtgaac aagttgatac tttgttaatt atttcaaata ataaccttct
    25681 tgagattgtt gataaaaaga cacctttatt agaagcactt agtgaagctg ataatgtttt
    25741 acgtcaggga gttcaaggga taaccgactt aattactagt cctggcctta tcaatctcga
    25801 ttttgccgac gtgaaaacag ttatggcaaa taaagggaat gccttgatgg ggattgggat
    25861 tggttctgga gaagagcgca ttgttgaggc ggcgcgtaag gcaatatatt cacccctatt
    25921 agaaacgact attgatggtg cacaagacgt tattgtgaac gttacaggag gtctcgacat
    25981 gacacttaca gaagctgaag aagcctctga aattgttggg caagctgctg gtcaaggcgt
    26041 taacatttgg ttaggaacat ctattgatga tactatgaaa gatgacatcc gtgtgactgt
    26101 cgtagcaact ggagtgcgcc aagaaaaagc cgaacaagtt tcaggttttc gtcagcctag
    26161 gacttttacc caaaccaacg cgcagcaagt agcgggtgca caatatgcat cagatcaagc
    26221 aaaacagtcg gttcaaccag ggtttgatcg tcgctcaaat tttgattttg acatggggga
    26281 gtctcgcgag ataccaagtg cacaaaaggt aatttctaat cataatcaaa atcaaggttc
    26341 agcttttgga aattgggatt tgagacgtga taatatttct cgtccaacag aaggtgaatt
    26401 agataaccat cttaatatgt caacgttctc agctaacgat gacagtgatg atgaattaga
    26461 aacgcctcca ttctttaaaa accgttaata atggatttac tgacaaataa aaagaagatt
    26521 tttgagacta tccgcttatc tacagaggca gcaaatagga ctaatgatag tgtttcagtt
    26581 attgctgtaa caaaatatgt ggatagtaca attgcaggtc agcttatcga agcaggaatt
    26641 gagcacattg ccgaaaaccg tgttgataaa tttcttgaaa agtatgatgc gttaaagtat
    26701 atgccagtaa agtggcattt aatcggtacc ttacaacgtc gtaaggttaa ggaagttatc
    26761 aattatgttg attattttca cgctctagat tctgtgagat tagctttgga aatcaacaag
    26821 agagctgacc atcctgtgaa gtgttttcta caagttaata tttctaaaga agagagtaaa
    26881 catggtttta acatttctga gattgatgaa gcgattgaag aaataggtaa gatggagaag
    26941 atacagttag ttggtttaat gactatggca ccagcaaatg ccagtaaaga aagtattata
    27001 actatttttc gacaagcaaa tcaattaaga aaaaacttgc agttaaaaaa agaaagaata
    27061 tgccttttac agaattgagc atgggcatga gtaacgatta tccaattgct attcaagaag
    27121 gctcaacttt tattcggatt ggtagagctt tctttcacta atggagaata agatggcttt
    27181 taaagataca tttaacaaga tgatttctta ttttgacacg gatgaggtta acgaagttga
    27241 agaagatgtt gcagcatcaa ctgataacgt tattccaaga tcacaacaat cagtcagagc
    27301 aagtagtcat ccaaaacaag aacctagaaa caatcacgta caacaagatc atcaagcgag
    27361 atcccaagaa cagacaaggt cacaaatgca tccaaaacat ggtacttctg aacgctatta
    27421 tcagcagtct cagccaaaag aaggccatga aatggttgac agaagaaaac ggatgagcac
    27481 ttctggtatt gcaaatcgcc gtgagcagta tcaacaatca acttgttcag atcaaacaac
    27541 tattgcctta aaatatcctc gtaaatatga ggatgctcaa gaaattgtgg atcttttaat
    27601 agttaatgaa tgcgttttga ttgattttca gtttatgcta gatgctcagg ctagacggtg
    27661 tttagatttt attgatggtg ctagtaaagt gctctatggt agcttacaaa aggtcggctc
    27721 ttcaatgtac ttactggctc cgtcaaatgt atccgtcaat atagaagaaa tgactatccc
    27781 acatactaca caagatattg gctttgattt tgatatgaaa aggcggtaaa taaatgatat
    27841 taatactatc tattcttctt cgtctgatca aagtttacac ttatttattg attgcttacg
    27901 cattaatgtc atggtttcct ggggcatatg attcaaaaat tgggcgtttg attagtggta
    27961 tcgttgaacc aattttaaaa ccttttagag catttaattt acaatttgca ggtcttgact
    28021 tcactatttt tgtcgtcatt attagtttga attttttagc tcaagttttg gtccgtgtgt
    28081 ttatttaatg gttagtcata gtaagattta tcagcatttt caccaagaag aatatccttt
    28141 tattgataga atgtctgata tgattaatag agttgaagat tactatcttt tagaagttac
    28201 tgagttttta aatcctagag aagtgatgat tttaaaaagt ttgattgctt taacagatct
    28261 aaaaatgttc gtatcaacag attactaccc aagcgaatat ggtcgtgtca ttattgcacc
    28321 tggttactat gacttagaac aaagtgattt tcaaatagct ttagtagaga taagttatca
    28381 ggcaaagttt aatcagttga cacatagtca aattttagga actttaatta atgaattagg
    28441 agtaaagcga aatttatttg gagatgtttt tgttgaaatg ggatatgccc agctcatgat
    28501 taagcgggag ttattggatt attttttagg aacaattact aaaatagcta aaactagtgt
    28561 gaaattaaga gaagttaact ttgatcagtt aattaggtct attgataaca gccagaccct
    28621 ggatattcta gtttctagtt ttcgattaga tggtgtagtt gctactatct taaaaaaatc
    28681 tcgaacgcaa gttatagcat taattgaagc aaataagatt aaggtaaact atcgactcgc
    28741 taataaagct tcagataatc tagtcatagg ggatatggtg agtatcagag gtcacgggcg
    28801 ttttactctt ttagcagata atggagtgac caaacatggc aaacaaaaaa taacactaag
    28861 taaaatgata cataaataag gagatacaat ggcacttaca acgctagaaa ttaaagataa
    28921 aacttttaag acaaaattcc gaggatactg tgaagaagaa gtcaatgaat tccttgatat
    28981 tgttgttgat gattacgaag ctcttgtacg taaaaatcgt gataacgaag caagaattaa
    29041 agatcttgaa gaaaaattat cttactttga tgaaatgaaa gagtcactta gccagtctgt
    29101 aatcttagct caagaaacag ctgaaaaagt gaaagcaacg gctaatgcgg aagcaacaaa
    29161 cttggttagt aaagcgactt atgatgctca gcatttatta gatgaatcta aagctaaagc
    29221 caatcaaatg ttgcgtgatg caactgatga ggctaagcga gttgcgattg aaacagaaga
    29281 actaaagcgt caaacacgtg tgttccacca acgtttaatc tcatctattg agtcacaatt
    29341 aagcttatca aattcacctg aatgggatga actgctacaa ccgactgcaa tctatcttca
    29401 aaattctgac gatgctttta aggaagtcgt gaagaccgtt cttaatgaag acattcctga
    29461 atctgatgat agtgcctctt ttgatgcaac acgtcagttt actccagaag agttagaaga
    29521 attgcaacgt cgtgttgatg aaagcaataa ggagttagag gcttaccaac ttgactctca
    29581 atctgattct atgactgagc cagaggtaaa tctcagtgaa acacaaacgt ttaaattaaa
    29641 tatctaaata aagaaaaaca agatgatttg cttataatca cagcaagcgg gagatgatga
    29701 gagtctcgta ttccctaagt attatcatta tcattttcta aaattttcgc tgaactacag
    29761 taagcgagga ggtttgttca ccttacgagc aaagagggag cggtaagtct tatgatatat
    29821 tatcgctctg aactaaggtg gtaccacgag ctttcgtcct ttttgagacg aggctttttt
    29881 cgttatagtc gcaagtttgt ttaaaaggag tttgaaataa tgaaattaaa agagacactt
    29941 aatctaggaa aaacagcctt tccaatgcgc gcaggtcttc ctaataaaga gccacaatgg
    30001 caagcagctt gggaacaagc agagctttac aaaaaacgtc aagaatcgaa tgcaggtaag
    30061 cctgcctttc atcttcatga tggacctcca tacgctaacg gcaatattca cgttgggcat
    30121 gcccttaata aaatttcaaa agatatcatt gtgcgttcta agtccatgtc tggctttcaa
    30181 gcgccttatg tacctggttg ggacacacac ggccttccaa tcgaacaagt cttggctaag
    30241 caaggaatca aacgtaagga gatggattta gcagaatacc ttgagatgtg tcgtcaatac
    30301 gctttaagtc aggttgataa acagcgaaat gatttcaaac ggttaggtgt ttcggcagac
    30361 tgggaaaatc cttatgtcac tttggaccca cagtttgaag ctgatcaaat tcgtgttttt
    30421 ggagcgatgg ctgaaaaagg ttacatctat cgtggtgcta aacctgttta ttggtcatgg
    30481 tcttcagaat cggccttggc agaagctgaa attgaatacc atgacatcga ttcaacctct
    30541 ttatactacg ccaataaagt taaagatggt aaaggcatcc ttgatactaa tacgtatatc
    30601 gttgtttgga caacaacacc atttacggtt acagcatcac gcggtttgac tgttgggcct
    30661 gatatggatt atcttgtggt gaaacctgct ggttcagacc gtcaatatgt tgtggcagaa
    30721 ggtcttttgg atagccttgc tggaaaattt ggctgggaat cttttgaaac tttagccagc
    30781 cataaaggag ctgatttaga gtacattgtg acagaacacc catgggatac tgacgtagaa
    30841 gaattggtta tccttggtga ccatgttacc cttgagtcag ggacagggat tgtccacaca
    30901 gcaccaggtt ttggtgagga tgactacaat gttgggacaa aatacaaact ggaagttgct
    30961 gtgactgttg atgaacgtgg cctaatgatg gaaaatgcag gtccagattt ccacggacaa
    31021 ttttataaca aggtaacgcc gattgtcatt gataaacttg gtgatctttt attggcacaa
    31081 gaagtgatca atcactctta cccatttgac tggcggacga aaaaaccaat catctggcgt
    31141 gctgtaccgc aatggtttgc ttctgtttct gatttccgtc aagatatttt ggatgaaatt
    31201 gaaaaaacaa ccttccatcc atcatggggt gaaacacgcc tttacaatat gattcgtgat
    31261 cgtggcgact gggttatttc tcgtcaacgt gcttggggag ttccgcttcc tatcttctat
    31321 gccgaagatg gcacagctat tatgactaaa gaagtgacag atcatgtggc tgatttgttc
    31381 caagaaaatg gatcaatcat ttggtggcaa aaagaagcta aagacctatt accagagggc
    31441 ttcactcatc cagggtcacc aaatggcgag tttacaaaag aaactgatat catggacgtt
    31501 tggtttgact ctggttcttc ttggaatgga gtcatgaata ctaaagaaaa tctttcctat
    31561 ccagcggatc tctacttaga aggctctgac cagtatcgtg gttggttcaa ttcatcctta
    31621 attacatctg tggctgttaa tgggcatgct ccatataagg ctattttatc gcaaggcttt
    31681 gttcttgatg gtaagggcga aaaaatgtct aaatcaaaag gcaatatcat ttctcctaat
    31741 gatgtggcta aacaatatgg tgcagatatt cttcgacttt gggtagcttc tgtggatacc
    31801 gataatgatg tccgtgtttc tatggagatc cttggtcaag tctctgaaac ttaccgtaaa
    31861 atccgtaaca cccttcgctt cttgattgct aatacatctg atttcaaccc tgctacggat
    31921 acagtagctt atgcagattt aggagctgtt gataagtaca tgacaattgt ctttaatcag
    31981 ttggtagcaa cgattactga cgcttatgag cgttatgact ttatggctat ttacaaagca
    32041 gtggtgaact ttgttacggt tgatttatca gccttctatc ttgattttgc taaagacgtt
    32101 gtctatattg aagctgccaa tagcttagaa cgtcgtcgca tgcagacagt cttctatgat
    32161 atcttggtta agattaccaa gttattgacg ccaatcttgc ctcacactac tgaagaaatc
    32221 tggtcttatc tagagcatga gtcagaagca tttgttcaat tggcagaaat gcctgtggca
    32281 gaaactttct cagctcaaga ggatatttta gaagcttggt ctgctttcat gactttgcgt
    32341 acccaagctc aaaaagcttt ggaagaagcg cgaaatgcta agattatcgg taaatcattg
    32401 gaagcccatt tgaccatcta cgctagcgaa gaagtgaaaa ccttattgac tgctttagac
    32461 agcgatattg ctttgctttt gatcgtgtct caattaacca ttgctgactt ggcagatgcg
    32521 cctgcggatg cagtggcatt tgaaggtatt gcctttatgg tagaacatgc cataggtgaa
    32581 gtttgtgagc gttcacgccg catcgaccca actactcgca tgcgttctta caatgcattt
    32641 gtctgtgatc acagcgctaa aatcattgaa gaaaatttcc cagaagctgt ggctgaaggg
    32701 tttgaagaga gtggcaaata agtcttatca agacttgaag tgacactgag taagaagaca
    32761 accatcgggt cttaggacct gatggttttt tagtagagct gattacgtcc attagccagc
    32821 tttaatgaga gacaaagtag gagaaattta gactactttt tttatttctt ttaagaaaat
    32881 tgctggcttc atataaggac tattaagata gagttcacct ttcgctaata aaaaaactta
    32941 gctcttttag aagggctaag ttttgtctga atagctagct gctgtttata cagaagtttg
    33001 aaatgaaatt tcaataaggt tattcgtgtg aagtgttttt gattgtccag gaataacaat
    33061 tttaggatca attttacgtt ttaaaaaaaa ttcgcgaatg atatcaagtt catcctcctt
    33121 gatggcacgg tatttatttg aaataagtag ttcgtaatgt tttggtgctt gggtaaaaat
    33181 gacatctgta tttcctgctg agtatacttc gactaaatag gcatcggtgt tttttagctg
    33241 atttctgatg agttctgggt gactgctagt ggtattgatg agtctcataa atgtcctcct
    33301 tagtattcct gtacctatta tagcactaat ttaaagcctt agttagtaga atgatgcttt
    33361 ttttgccatt ccttgactcc ccattgatgg ctctctcgtt ttaatttagc aattgcctcg
    33421 ataggagaaa accaacctaa gttattaaaa tcttctaagg gcttagaaac ggcttgaaat
    33481 gcagttactt cataaagata ggcaggatgg tagaagtggg tatcacgatg gcgagagtag
    33541 aaatattcat ctgcctgacc gtaatatgaa ccaatagtag cagaaaaacc aagttcttca
    33601 atcagttcac gctctagggc ctgaagctga ccttcacctg cttcaatttc accaccaggt
    33661 aaaaaccagg aaccattagg agcttgaaca agaataattt ttgtttgttc gtgattggga
    33721 atgatagcat agacaccgta acgtgtgaca tagtctttat gtgcattttt atgtccaaat
    33781 gttggaatca tcattttatt tacctttcct ttttcttcat tatactaaat ttttgctaat
    33841 tattcttatc aatttgttta gtttaataaa aaattgatag aaaaagagag agtctcctta
    33901 tagaaactct cttagctaac gttaatcgag agactcttcg ttaagaaaag aatctccctc
    33961 tttaacagct ttaatcgtga tgttttcact gtttgtcatc agtgcacgta actgtttttc
    34021 agttgggtgt tctaaataat agtctgtaat ggcatcttcg atataatctt ggatagtacg
    34081 acggagcggt cgagcgccca ttttaggatc atagcctaaa tctactaatt tttctttgac
    34141 cttttgtgtc acatcaagat gaatcccatt atagcctaag cgctcattaa catcttccag
    34201 cattaaatca acgatatgca gcaaatgctc tttagataaa gctttgaatt caataatacc
    34261 atcgaaacga ttcataaact ctgggctgaa gaagttgctt aattcaccaa gaacagaact
    34321 tgtccgtcct tctctagcag caccaaatcc gacagaagct tcgcttttac ctgttccggc
    34381 attagaggtc atgataataa tggtgtcctt gaagctgact gttcgtcctt gaccatctgt
    34441 taaacggcca tcatcaagaa cttgtaagaa catgtgcatg acgtcaggat gggctttttc
    34501 cacctcatct aagagaataa gtgaatatgg attgcgacga acttgttcgg ttagctgtcc
    34561 agcttcttca tagccgacat aacctggagg agccccgact aatttggcga cagcgtgttt
    34621 ttccatgtat tcggacatgt caaagcgaat catattgttt gtcgaaccaa agagttcaat
    34681 ggctagttgt ttagacaatt cagttttacc aaccccagtc ggtccaacga ataagaaaga
    34741 accaataggg cgatttggag ttcctaatcc cacacggtta cgacgaatag ccttagcgat
    34801 tttatcaaca gcatcgtctt gcccgatcac gtgtgctttc agatcattgg ctaagtttac
    34861 gagttgagac tgttcctttt cttttaagtc cccaactgga atattagttt tttgctctac
    34921 aatagcttca atggtttttt cagtaatgat gggaatatct tgctcatcga ctttttgagc
    34981 ctgcatttct ttgtattttg taatttgatc gcggaaataa gctgcgcgtt catagtcttc
    35041 gtctcgagta gcttgcgcct tgagattctc agcttcaata agacgtttgt caatttcttt
    35101 aggatcaaca aagttgaggg ttagattcat tttggaacca gcttcgtcca gaagatcaat
    35161 agccttatca ggaagaaaac ggtcttgaat atagcggtta gataaatgag cggcagcttc
    35221 aatagcggct gggctatatt tgacatgatg atagtcttcg tatttcggtt ggataccttt
    35281 taaaatcgtg atggtttcct caacagaagg ttcatcaact ttaacgggtt gcatacgtcg
    35341 ctccaaggca gcatcttttt caataatacg gtattcattt aaggtagtag caccaacgag
    35401 ttgcaactca ccacgggcca aggctggttt taaaatatta ccagcatcca tattgccgtc
    35461 tcctgcagaa ccagcaccga caatctcatg aatttcatca ataaagagaa tcacatcctt
    35521 gcgattgcga atttcttcca ttaatttttg catgcgctct tcaaactgac cacggatacc
    35581 tgttccctga acgaggctaa ccacatcaag acgaatcact tgcttgcctt ggagtttttg
    35641 aggaacagta ccatcaataa ttttttgagc caaaccttct acaacagcag ttttaccaac
    35701 tccaggctca ccaattagca caggattatt tttagtacgg cggttgagga tctcgataac
    35761 gcgtgtaatc tcttcgtcac gaccaataac aggatcaata ttaccatttc ttgcaatatc
    35821 tgtgacatta atcccaaact cttctaataa gccctttgct tgctgatttg gtgtttgtgg
    35881 ctgagcaggt cgttgtccgt tgtagttacc accatagcgt cctccgccat ttccattttg
    35941 ccctgcctga gtaggtgggg tatttggaag attaccaaaa gctctaaaat tattcaaatc
    36001 accaaaaaag tcatcaaaga aaggacttgt ggatctatct tgtgctctat atcctggggt
    36061 taggccattt aaaatagaat tagtaggatc ggatttcatg atttgataac aattttgaca
    36121 gagatcaacc tgtctttgtt ttccattaac acttgtataa agatgaatag tagattcgtt
    36181 taaattacaa ttttgacaca gcataatata cctccgaggg tttatctaaa agtcaaagtc
    36241 atttagtcaa aaatggtcaa actttatatt catattatag caccacggct tgggaaatca
    36301 agtaaaaagt ttatcgcttt agcgttagct tttagagagt aacatgacag agtaacttta
    36361 ttaagtttcc aatgagcaaa tatttaaaat cattgaaaag agtaaaaaaa agtaaaatat
    36421 caataaatat attactttcc ttgttagttt cccttgtttt gtgttaaaat aagagggata
    36481 aaaaacgaat aggagaaacg aaatggaatc acatttggtg agaatcatca atcgtttgga
    36541 gttgatggtg acagatggcg gtaatctcaa gcgaaatttt gaacgcgatg gtgttgttgt
    36601 tgcagaagtt gccttttcaa atgatcctga aaatggacct gttttcacat tgcgtgatgt
    36661 tgaagcgcgt gagtcatatt cctttgatag cattgacttg attgcaatgg aaatctacga
    36721 tttattatat taagcttgac atagtaaaga gacttgcctg aggcaggttt ttttattgtc
    36781 aaaaagtatt gtattaatat tcatcagatg gtataattat ctttattgta atgaacctag
    36841 gacttatgag gagatataaa tggatttgtc atttttgccc aaatactggg cctactttaa
    36901 ctacggtgta cttgtcacca ttatgatttc aatcagcgtt gtcttttttg gaacccttat
    36961 tggtgtcttg gtaaccctga ttaagcgtag tcatgtgaag ccgttgacct gggtcgttaa
    37021 tctttacgtt tggatctttc ggggaacacc tatggtggtt caaatcatga ttgcctttgc
    37081 ttggatgcat tttaacaata tgcctactat tggttttggg gttttagact tggacttttc
    37141 aagactactt cctggaatta ttatcatttc attgaatagc ggtgcttata tttcagaaat
    37201 tgttagagca ggtattgagg ctgtaccaaa agggcaatta gaagcagctt attcactagg
    37261 tattcgtcct caaaatgcca tgcgttatgt gattttgcct caggccttta aaaatatttt
    37321 gccagcctta ggaaatgaat ttattactat tattaaggat agtgctcttt tacaaaccat
    37381 tggagtgatg gagctttgga atggtgccca atcagtggta acggctactt attctccaat
    37441 ttccccttta ctggtggctg ctttttacta cttaatgatc acaacagtga tggcacagtt
    37501 attggcagtc ttagaacgtc acatggcgca aggaggtaat cattgatgac tgaggctatt
    37561 atttcaatta aagatttgca caagtattat gggcacaatg aagtgcttaa gggaattgat
    37621 ttagacatta tgccaggaga ggttgtggtg attattggcc cttctggttc tggaaaatcg
    37681 accttacttc gaactatgaa tcttttggaa gtaccaacta agggacagat taggtttgag
    37741 gggattgata ttaccgataa gaagaatgat attttcagca tgcgtgaaaa aatgggaatg
    37801 gttttccagc agtttaacct ctttcccaat atgactattt tagaaaatat cactttatcg
    37861 ccaatcaaaa ctaagggaat ggctaaagca gaggctgaca aaacagcctt gagcttgttg
    37921 gacaaagttg gattatcaga aaaagccaag gcttatcctg ctagtctttc tggtgggcaa
    37981 cagcagcgga ttgcgattgc gcgtggactg gctatggatc cagatgtttt actctttgat
    38041 gaaccaactt cagctctaga cccagaaatg gtgggtgagg tcttggctgt catgcaagat
    38101 ttggctaaat ctgggatgac tatggttatt gtgactcatg aaatgggctt tgctaaagaa
    38161 gtcgcagatc gtgtgatttt tatggatggc ggcgttattg tggaagaagg aagtcctaac
    38221 caactttttg atctgactaa agaagaacgt acaaaagatt ttttgagtag agtattataa
    38281 atctgttcta tgaattataa aaggaagtcc taaatcattt aggacttcct tttataattt
    38341 gctaaggcat tttataggag attacttttc tttttgttaa aataaaaaga aatagttgca
    38401 ttatttgcat agaagaaagg gatctgttat ggtttcagtg atgtggtttc gtagagattt
    38461 aaggattgat gatcaaaaag ctttagctca tgctattgct agtcaatcac caaccttatg
    38521 tgtgtttcaa tttaacaagg agcaattaaa tctgctacat agtcggaatc aatctagttt
    38581 tattactagc gttttggctt ttagaaagtt attaaaatgt gaaggtattg acttatatct
    38641 aatgtatgga gacttaatga cctgttttga gcaattattg acacaattaa aagactggac
    38701 agatgtgtat tttaactatg atgaatctgg atatggtcgt ttacgtgatc aaaaagcagc
    38761 acaatttttc aaaaaaaatg ggattgctgt tcatacctac caaaatcact atttacatgg
    38821 tagtcaagag atcattaacc aatcaggtca gccttataaa gtttttacgc cctactatag
    38881 gatttggcaa aattatccga aagagactcc tattaaagta gaactatcgc aaggcaggtg
    38941 gttaaatctt gagacaccag atgatgtttt aaggactgtt gagagtttta aagatgaaaa
    39001 atatcaggat gttgcaactt ttgatgaagc gtcaaaacaa ctaaatcgat ttattcaaga
    39061 ccaattagca gcttatcacg ctaatcgaga ttttccagcg cagctaggga cgagtcggtt
    39121 gtctcctttt ttaagaatag gagctattgg cattcgcacg gtatatcacg ctgtgcgtca
    39181 agcaccaaat agtctagggc aagctacttt tttaaaagaa ttagcttgga gagactttta
    39241 caatatggtc tatgtggctt atcctgatca gaaaactcag cctatccaaa aagccttttc
    39301 tcaaatcgaa tgggttaata atcctgactg gtttcagttg tggaaagaag gtaaaacggg
    39361 ttaccctatt gttgatgctg ccatgcttca gttacaaaag acaggatgga tgcataatcg
    39421 tttacgaatg atagtagctt cttttttaac taaagatctt ttatgtgatt ggcgtttagg
    39481 agagcagtat tttcaacaac aactaattga ttatgatgct gccagtaata tcggaggatg
    39541 gcagtgggct gcttccactg gaacagatgc tgttccttat tttagaatat ttaatcctgt
    39601 tactcaaggt aagcgttttg atcccaaagg cgagtttatt aaagcgtatt tgcctcaact
    39661 agaacacgtt ccagagaagt atctccacga accgtggaag atgccaaaat accttcaaga
    39721 gagtgtatcc tgtattattg gcacagacta tcctcaacca attgtcgatc atgctaaaca
    39781 acgtgaacaa gctattgcaa agtatgagtg ggcaaaagaa aaagcaaaaa tagaatgact
    39841 gttctcatta taagagttta aacaaaaagt tatcatgaat taagattatt taatttaaaa
    39901 aataagttga ctttatttag attatttgat actattcagt tgtctccctt agagaaagcg
    39961 atttcttgtt ttggagctga tttttaccat ttggtcttag aataggatga gtaatatgac
    40021 ttacaacgag gtatatcaag aatggttgca caataatgat cttagtgatg atattaaagc
    40081 agatttagca gccataaaag acaatgaggc tgagattcaa gatcgttttt acaaaacact
    40141 tgaatttgga acagcagggc taagaggaaa acttggagca ggcaccaatc gcatgaatac
    40201 ctatatggtt ggtaaggcag cacaagcttt agctaatact attattgatc atggacctga
    40261 agcggttaaa aagggcattg cggttagtta tgatgttcgc tatcaatcta gaacatttgc
    40321 agaattaaca tgctctatta tggcagctaa cggtattaag gcgtaccttt ataaagggat
    40381 tcggccaaca ccaatgtgtt cgtacgctat tcgtgctttg ggatgtatct ctggtgttat
    40441 gattacagct agtcacaatc cccaagctta caatggttat aaagcttatt ggcaagaagg
    40501 ttctcaaatt ttggatgaca ttgcggatca aattgcacaa cacatggctg cactaactca
    40561 gtatgaggaa atcaaacaaa tgccttttga aaaggctctg gacagtggac ttgttactta
    40621 tattgatgag agtattgaag aagcatataa aaaagaagtg cttggtttaa cgattaatga
    40681 tactgacatt gataagtcag tccgagtagt ctatacccct ttaaatggtg ttgggaatct
    40741 accagtgcgt gaggttttaa gacgccgcgg ttttgaaaat gtttatgtgg tacctgagca
    40801 ggaaatgccc gatcctgatt ttacaacagt tggctatcct aatcccgaag tccctaaaac
    40861 ttttgcatat tcagaaaaat taggtaaggc agttgatgct gatattttga ttgcaactga
    40921 tccagattgt gaccgtgtgg ctttagaagt taaaaatgca gtcggtgact atgtttttct
    40981 caatggcaat aaaatcggag cgcttttatc ttactatatc ttttcgcaac gatttgactt
    41041 aggcaattta ccggctaatc ctgttttagt gaaatccatt gtaacaggag acttgtcacg
    41101 ggctattgct agccattatg gtattgaaac cgttgaaaca ttgactggtt ttaagaatat
    41161 ttgtggcaag gcaaacgaat atgatgtgac gaaacagaaa aactacctgt ttggttatga
    41221 agaaagtatt ggtttttgct atggcacttt tgttcgtgac aaagacgctg ttagcgcctc
    41281 tatgatgatt gtagaaatgg cagcttacta caagaaaaag gggcagaatt tactagatgt
    41341 tttgcagact atttatgcga catttgggta ttataatgaa cgtcaaattg ccttggagct
    41401 tgaggggata gaaggacaaa aacgcattgc gaggataatg gaagatttca gacagacacc
    41461 aatagctagt gttgcagaga tggcgttaga caagacaatt gattttattg atggctatca
    41521 agattttcca aagcaaaact gtctcaaatt ttatttggat gatggttctt ggtatgcact
    41581 tagaccatca ggaacagagc ctaaaataaa attttattta tatactattg gccaaacaca
    41641 agaaaatagt gcaacaaaat tagatgctat tgaagcagcg tgtcgcacaa aaataaatca
    41701 ggttaattaa acatcaaaaa tggataacac ttttacacta ttttccatat aaacaactac
    41761 aaaaggaggt gagactttta ggcaccttct ttttcttgta ggtgccacta atcaatttaa
    41821 tcgactcaaa tcaatactaa aaaataagta agagtataaa gaacaggcat ctattttttt
    41881 gttataatag aaaaatgaaa ttaaagggag atgtcaatga cagaactaat tgatggtaaa
    41941 gccttagctc aaaagatgca acaagagtta gcagctaaag tcaacaatct aaaacaaaaa
    42001 aaaggaattg taccaggctt agccgttatt cttgtaggtg atgatcctgc tagtcaggtg
    42061 tatgtccgta ataaagagcg tgcagctctt actgtaggtt ttaaaagtga gaccgttaga
    42121 ttatcagaat tcatttgtca agaagagctt attgcagtaa tcgaacgtta caatgcagat
    42181 aacactattc atggtatttt agtgcagcta cccctgccaa atcatattaa tgataaaaaa
    42241 attattctcg ccattgatcc caaaaaagat gtggatggtt ttcacccgat gaatacaggt
    42301 cacctttggt caggacgtcc cttaatggtt ccttgtactc catcagggat tatggaattg
    42361 cttcgagaat ataatgttaa ccttgaaggt aaacatgccg tcattattgg cagatcgaat
    42421 atcgttggaa aaccaatggc acagctctta ctagacaaaa atgcaacagt cacgttgaca
    42481 cattcaagaa cacgtcaatt agaagaagta tgtcgctgtg cagatgtgtt gattgtggca
    42541 atcggacaag gtcatttcat aacaaaacaa tatataaaag atggtgcgat agtgattgat
    42601 gtgggaatga accgtgatga taatggcaag ctaattggag atgtggcctt tgatgaggtg
    42661 gcagaagttg cagcgaaaat cacccctgta ccaggaggtg tcggtcctat gacgattgct
    42721 atgttgctag agcaaactta tcaatctgct ctccgtagta ctcataaata gttatcatta
    42781 gacaattcaa cataagagaa tataagaagt cagcaatggc ttcctttttt gtttttctgc
    42841 tcactattta tccagaaaaa tggtataatt gcattagtat gcaaagggac tatgtcaggt
    42901 ccaactaagt gaaaggtggc ttgatggcag attatttaac cgtcactcat ttgacgaaat
    42961 atttaaaatt aaaatttgac cgtgaccctt atttggaacg ggtttatctg actggtcaag
    43021 tgtccaattt tcgaaaacga ccaactcatc aatatttttc cttaaaagat gaaagtgctg
    43081 tgattcaagc caccatgtgg gcaggagtct ataaaaaact aggatttgac ctagaagaag
    43141 gcatgaaaat taatgtgatt gggcgagtcc aactttatga accgagtggt tcctattcta
    43201 tcgtgattga aaaggcagag ccagacggca taggtgcctt ggctttgcag tttgaacaat
    43261 taaagaaaaa attaactgca gaaggttatt ttgagcaaaa acacaagcag ctcttaccac
    43321 agtttgtgtc aaaaattggg gtcattacaa gtccaagtgg cgctgtgatt cgagatatta
    43381 tcacaactgt ttcacggcgt tttccagggg ttgagatttt attattccca actaaagtac
    43441 aaggtgatgg agccgcccaa gaagtagtgg cgaatattcg aagagccaac caaagagagg
    43501 atttggactt gctcattgtt ggccgtggag gtggctcgat agaagacctt tgggctttca
    43561 acgaggaaat agtagttcag gctatctttg aatcgcaact tccagtgatt tcgagtgtgg
    43621 gtcatgaaac tgatacaacc ctggcagatt ttgtggcaga tcgcagagca gccacaccaa
    43681 cagcagcagc agagttagca acgcctatta caaaaacaga tcttatgtct tggatagtag
    43741 agcggcaaaa tcgttcttat caggcctgct tgaggcggat taagcaacgg caagagtggg
    43801 tggataaact atcacaatct gttattttta ggcaaccaga acgattatat gatgcctact
    43861 tgcaaaaaat tgatcgcctt agcatgaccc tgatgaatac gatgaaagat cgtctgagct
    43921 ccgccaaaga aaacaaggtt cagttggacc atgccttggc caatagtcaa ttacagacaa
    43981 aaatcgagcg ataccaagac cgtgttgcta ccgctaagcg tttactgatg gctaatatgg
    44041 ctagtcaata tgatagtcag ctggctcgct ttgaaaaagc acaggatgct ttgctatcac
    44101 tggatgccag ccgaattatc gcccgtggtt atgcaatgat tgaaaaaaat caagcactgg
    44161 tagcctctgt aagtcagata acaaagggtg atcagttaac cattaagatg cgtgatggac
    44221 aattagatgt agaggtaaaa gatgtcaaaa acgaaaacat ttgaagaaaa tttacaagat
    44281 ttagagacta ttgtgaacaa acttgaaaat ggggatgtcc ctttggaaga agctatttca
    44341 gaatttcaaa aaggaatgct tctctcaaaa gaacttcaaa agaccttaca agcggcagaa
    44401 aaaacactcg tcaaagtgat gcaagctgat ggcacagaag tagatatgga tgattaatgg
    44461 acaaattagc tagaattgac gaagccattc gtcgctacta taagacgact agtaacggtg
    44521 tatctgaaga gttgattgat gctatcttgt attctgttga cagtggtggc aagcgcattc
    44581 gaccacttat cttattagag atgattgagg gatttggggt atcgttacag aatgctcatt
    44641 ttgatttggc tgcagccctt gaaatgattc atacaggaag tctgattcac gatgatttgc
    44701 cagccatgga taatgatgat taccgacgtg gtagactgac caatcacaaa caatttggag
    44761 aagctacggc cattttggcg ggagatagct tatttttaga cccctttggg ttaatagctc
    44821 aagctgaatt aaatagcgag gttaaagtag ccttaatcca agaactttcc ctagcttcag
    44881 gaacttttgg catggttggt ggtcagatgc tagatatgaa aggggaaaat caagctttga
    44941 gtctccctca gttgtcactg attcacctca ataaaaccgg aaaattatta acttttcctt
    45001 ttaaagcagc agctcttgta acagaacagg ctattactgt tcgtcaacaa ctagagcagg
    45061 cgggaatgct catcgggcac gcctttcaga ttagggatga tattttagac gtgacagcta
    45121 gctttgaaga tcttggaaaa acgcctaaaa aggatttatt tgcagaaaaa gctacttacc
    45181 caagtttact ggggttagaa gcctcctacc aactgttgac agagagttta gatcaggctt
    45241 tgacgatttt tcagacactc gaaagcgatg taggctttaa gcctcaaata attacaaaac
    45301 tgatagaagg gttacgactt aatgcctaaa gaaagagtag atgtgttagc ctataagcaa
    45361 ggattatttg agaccagaga gcaagctaag cgtggtgtta tggcaggctt agtggtctct
    45421 gtgattaatg gccaacgcta tgacaagcca ggtgacaaaa ttgacgatgg gactgagtta
    45481 aaacttaaag gtgaaaaatt caaatacgtc agtcgtggtg gattaaaact ggaaaagggg
    45541 ctgcacgttt ttggtgtatc ggttgctaat caaattggga ttgatattgg cgcttcaaca
    45601 ggtggtttta ccgatgttat gttacaagat ggggccaaac tagtctatgc cgtcgacgtt
    45661 gggactaacc agttggtatg gaaactcaga caagatccac gagtaagaag tatggaacag
    45721 tataactttc gttatgctca gccagaggac tttaatgagg gacagcctgt atttgccagt
    45781 attgatgtgt cctttatttc tctcagcttg attttgccag ccctgcataa cgttttgtca
    45841 gatcagggac aggttattgc tctcattaaa ccgcaatttg aagctgggcg cgagcagatt
    45901 ggtaaaaaag gcatcgtcaa agacaaacag attcatgaaa aagtgattca aaaggtcatg
    45961 gattttgcct caggttacgg atttacagtt aagggacttg atttttcccc tattcagggt
    46021 ggtcatggca atatcgaatt tttagctcat cttgctaagt cacagacacc tgaaacgtta
    46081 gccccgcatt tgattcagaa agttgttgcc aaggcacata aggagtttga gaaacatgaa
    46141 aaagagtgaa cgacttgaat taatcaaaaa aatggttttg acgcatccaa ttgagacgca
    46201 acatgatctc ttaagattat tggcagagca tgggttagaa ttaacccagg ctactatttc
    46261 aagagacatg aatgaaattg gtatagtaaa gattccttct ggcagtggac gttacatcta
    46321 tggtctttct caagatagtg gaaaaaagat cgttcaagga cctagatcga taaagagcac
    46381 tattttagct gtatctgaca aaacaaaagg tttagaacaa cacctctatt taaaagtcgt
    46441 acctggcaat agtaaattaa tcaaacgtta tttattagca gatttttcaa aagctatttt
    46501 tagtcttatt gctgatgatg atagtttact gttaattgca aagtctcctt cagaagcaga
    46561 tatgatacgc caagaaattt tgctttggat gcagggtata acctagaaaa aaatagggga
    46621 gaaagtgatg ttattagaaa tttctattaa aaattttgct attattgatg aaatttccct
    46681 aaattttgaa aatggtatga cggttttgac gggtgaaact ggagcaggaa agtctattat
    46741 cattgatgct atgaatatga tgcttggtgc gcgtgctagt acagaagtga ttcgtcgtgg
    46801 agctaataaa gcagaaattg aggggttctt ttcggtagat gctaccccag aactggttgc
    46861 gtgtctggaa tcatcaggta ttgctatgga agaagaactg attattcgcc gagatatctt
    46921 tgccaatggc agaagcgtga gccgcattaa tggtcagatg gttaacctag ccaccttaaa
    46981 acaggtcgga cagtttttgg tagatattca tgggcaacat gaccaagaag aattaatgcg
    47041 accacagctc caccagcaaa ttttggacgc ttttggtgat aaggcttttg agcaattgaa
    47101 agagaactat cagcttattt ttgatcgtta taaaagcttg cgtcgccagg ttattgacaa
    47161 acaaaaaaat gaaaaggaac acaaagatcg tattgatatg ttggcctttc agatagcaga
    47221 aattgaagct gctgccttga gtcgaggtga ggacgaccgg ttaaatcaag aacgtgatcg
    47281 cttaatgaac cacaaacaaa ttgctgatac cctgaccaat gcctacgtca tgctagataa
    47341 tgacgatttt tcaagtctat ccaatattcg ctctagcatg aatgacttac tatcaattga
    47401 gcagtttgat tcagaataca aagggatgtc gacttcgatt tctgaagcct attatattct
    47461 tgaagaagtg agcaagcaat tatcagatac cattgaccaa ctggattttg atggtgggcg
    47521 attacaggaa attgaatttc gcttggatac actcaatagc ttaacccgta aatacggtgg
    47581 taatgtgaat gatgtgcttg actactacga taatatcgtc aaagaatacc agctattaac
    47641 aggcgatgag ttgtcttcag gggatctaga ggcagaattg aaagccttag aaaaacaatt
    47701 ggttgctgct gcttcggagt taagtgtctc tcgccatcaa ttggcggagc aattagaagc
    47761 tgagattaaa gctgaactta aggaacttta tatggaaaaa gcagatttca aagttcactt
    47821 taccacatca aaattcaatc gtgatggtaa tgaaagcttg gaattttaca tctccaccaa
    47881 tccaggtgaa ggattcaagc cccttgtcaa ggtggcttcg ggcggcgaat tatcacgctt
    47941 gatgttggcc atcaaggcag ctatctctaa aaaagaagat aaaacgagta ttgtttttga
    48001 tgaagtagat acaggtgttt ctggtcgagt tgctcaagcc attgcccaaa agatttataa
    48061 aattgggcga catgggcaag ttctcgctat ttcacactta cctcaagtca ttgccatcgc
    48121 tgattaccag tattttattt ctaaagagag taaagaagag tccactgttt cgaaggtaag
    48181 gttgttaact tctgaagaac gtgtggaaga aattgccaga atgcttgctg gtgcagacat
    48241 gacagaagct gctctcactc aggctcgtga attactggcc aaacattaat aaacagaaaa
    48301 aactgtgact attcaataga atagtctttt gataagaaag agaaaccgtc ggattagcct
    48361 gttgttagat tctggagcct tagcgctccg atgccatcta gcaggacgtt tggcggtttc
    48421 tttttgctca actgtcttga caaacccctg ataaatagta gaatatataa gagaatttta
    48481 agctgagggg atagaaatga ctagtaaaaa agcgtgttta tcaagtatca ttgtgttagc
    48541 aagtttaacg tgtggaaatg atactgttag tgccaatcat ctctcagcaa ctggagataa
    48601 gtttgatgat tgctcaacac ttgttgaaaa agatgtggcc cctaaagatg aacttgagat
    48661 gttagcatgg tcctcgtctc aaacaactga tgatgctgac agagactatg aagattttct
    48721 cgatgatgat tcttttaatg atgattcttt tatttctcaa aatgaaactg ataagatgtt
    48781 tgagaattta actgatgata ggttattaaa tgaattagat gaattagatg aattagatga
    48841 agaagataca attgagccag agcaaaatgt aataatgcct agtgacgatg agctatttga
    48901 tttaactgat gctgttgaga cacgccttac tgtttctagt gctccccatt tagaggctga
    48961 attgccgaaa ccacatttga ggagcctatc agatacagca ctgcggtctg gtgaaattag
    49021 aggacattta gataacaaac tggacacttt gtctgtaaca gctacaaagt tagcattaac
    49081 gatggctcaa aaatttgatt tgacaacgca tgtctattct ataggtgaaa gctttagtga
    49141 agtattagct gctcattatg aagacagaaa agcagaatca gctttttcta agaaaaagag
    49201 atttcacctt cctattgcta ctccagatgt tgttatagag gagttaaggc gcctagtctc
    49261 ttctattgga agttcaaaag aagatgtttc agttccttat aatcggaagc taggtatggc
    49321 agttgcaaaa agaaaaatag ccctgccaca aacgggagag gagttctctt attatccagc
    49381 tttacttggt ttaatgatat taggattaac gccgattatg ataccaaaga agataaataa
    49441 ttaggtaata agttattgta gttgtaaaag acggggcaaa tgttcaagcg tgaaaaggtt
    49501 gacttttgcc tcgttttgta tctcgttatt gctaaggcat tttcttcgag cagctagtgc
    49561 tcgccatacc tatctaccga gtgattattc aatagcacta ccagttttgc tttatttagt
    49621 aaggagtgtt agaataaaga ggtgagaaac tagtttagaa tggaaaaata catgggaact
    49681 ataaaaattg ttacagattc atcaataact attgaaccag aattaattaa agctttagat
    49741 attactgtag tgcctttatc tgtcatgatt gatagcaaat tatattctga taatgattta
    49801 aaagaagaag gtcatttttt gagccttatg aaggctagca aatcccttcc caaaaccagt
    49861 cagccaccgg ttggtctctt tgctgaaact tacgaaaatt tggtcaaaaa aggagtaact
    49921 gacattgttg ctattcacct ctcaccagct ttatcaggta ctattgaagc ttcccgtcag
    49981 ggggctgaaa tcgctgaggc acctgttaca gttttagatt caggatttac tgatcaagcc
    50041 atgaaatttc aggtcgttga ggcagcaaag atggcaaaag cgggtgctag tttgaatgag
    50101 attttggctg ctgttcaagc tatcaaatca aaaacagagc tctacattgg tgtatctacc
    50161 ttagaaaatt tagtaaaagg aggtcgtatt ggtcgtgtga cgggtgtttt gagctcgcta
    50221 cttaatgtca aagttgttat ggcacttaaa aatgatgagt taaaaacgct tgtcaaaggt
    50281 cgaggtaata aaacatttac gaaatggtta gatagttacc tcgctaaaaa tagtcatcgc
    50341 ccgattgcag agatagctat ttcttatgct ggtgaagcta gcctggcatt aaccttaaaa
    50401 gaaagaatcg cagcttatta caaccactct atttcggttt tagaaacagg ctctatcatc
    50461 caaacacata caggagaagg agcttttgcg gttatggttc gttatgaata atcgtcattt
    50521 atttagtggg atatttttct ttgttattag tttatgtctg gcctttttat tgctaaatat
    50581 tattatccct aagtcaaatt cacgtttgaa aaagagtgat tttctgaaaa aagaacaagt
    50641 agctatccaa tatgttgcta taggagattc attgacagaa ggagtaggtg atctaactca
    50701 tcaaggtggt tttgttcctt tgttaacgaa tgatctcagt gaatatttta aggctaatgt
    50761 taatcatcaa aattacggcg tatctggtga taccagtcaa caaattcttg ataggatgat
    50821 aaaacaaaag cagatacagt tatctttaaa aaaagcagat ataatgacgt taaccgttgg
    50881 tggtaatgat gttatggcag ttattcggaa gaatttagcg gatttgcaag tttctagttt
    50941 tagaaagcca gctcgtcagt atcaaaaacg attaagacag attatcgagt tagccagaaa
    51001 agataataaa gatcttccta tttttatttt aggcatctat aatccgtttt atttgaattt
    51061 tccagaacta actgatatgc aaaaagtgat tgatgactgg aataccaaaa ctaaggaggt
    51121 tgttggagaa tacgatcgtg tgtactttgt gccaataaat gacctcttgt ataaagggat
    51181 aaatggacaa gaaggaattg tccattcttc aggagatcaa actacaattg tcaatgatgc
    51241 cttgtttact ggggaccatt ttcacccaaa taatactggc tatcaaatca tgtcaaatgc
    51301 agtaatggag aaaataaaaa agcatgaaaa aaaaatcaaa ccttaattgg tggaagtgga
    51361 gcttcttatg tctgcttgct tttaatactg cctttttgat ggttattgca agtcgtctca
    51421 ttcaagtaag agaacctgag tcagaactga ttgctaaaaa accagttaaa aatatcaaaa
    51481 ttggtacatt tgtcacaacc agagaacagc ttaacgaaac tgtagcaagt tatcttaaag
    51541 attatcagac taaaaaaatg tcttataaat tttatgcgac atcttcttct attttgtttg
    51601 aaggaactta tcaattatta gggtatgaag tccctttata tatctatttt caacctcatc
    51661 gtctagaaaa tggagctgtc caactacagg tgatctcttt ttcagtggga actctgccac
    51721 tcccagaaaa agatgttttg cagtatttga aatcaagcta caaattgcca agctttgtta
    51781 aagtgatgcc aaatcaatca gctatcgttg ttaatttaca agatatccaa aatgatgcta
    51841 aggtttattt aaaagccaag aaaattgatt tatttaatga tgaaatcagt tttaacatct
    51901 ataagaaata agctcaaata acgttaaata cttgacctaa tcgctttttt tagatatgat
    51961 aatatctgtt aaagcattaa gcttactaat tatttggagg atttgttaaa tggctaacaa
    52021 acaagattta atcgcaaagg ttgcagaagc aactgaattg actaaaaaag attcagcagc
    52081 agcagttgat gccgtgtttt ctacaatcga agctttcctt gctgaaggtg aaaaagtaca
    52141 attgatcggt tttggtaact tcgaagtacg cgaacgtgca gctcgtaaag gtcgtaaccc
    52201 acaaactggt gcagaaattg aaattgcagc ttcaaaagtt ccagccttca aagctggtaa
    52261 agctcttaaa gacgctgtta aataattcgt ctagaaaaat cttgttgcta tcgatgttta
    52321 ttgatagcga caaggttctt tttttatatt tggggcataa agttagtagt tttctcttca
    52381 gttaagccaa gttctaaagc atgcactcta taatggtcag tcatccttct attagtatat
    52441 catttccgca ggtaggggga gtttttttca tatttgcaaa tagttgaagt taggattttt
    52501 gaaagcaaaa ggtaagttta tttagaatgt ttctaaatta cttgacttat ttatttagaa
    52561 ttattataat taatatatct taaagattga ataactttta attgcgaggg aactaaaaat
    52621 gacaatcaga caatggatgg cagaccatct tcatttgatg gagactttag cgtgtttggt
    52681 attaataata attggcctag cctttttaca ctcatttcca caagtggctt ccgctatttt
    52741 cattacagcc ttcttgattg gaggatatac gtctgcaaaa acgggtatat tggatttggt
    52801 gaagaacaaa cacttgtcag tggatatttt gatgattttg gcagctatcg gtgctggaat
    52861 tatcggctat tggctggagg gtgctctgct tatttttatc ttttcgttgt ccaatacgct
    52921 tgaagaaatg gccatggaaa aaagtaagga tgctatttca gccttgatgt ccttgacgcc
    52981 agatacagct cgtcaatacc aagaagatgg ccatatttta gaagttgaga ccagatcctt
    53041 gagtgttggt gaccgtttac aagttcgtaa aggcgaagct gttccaattg acgggcagtt
    53101 gcttagtcct tttggtcaat ttgatgaatc tatggtcact ggtgagccta tcactgttga
    53161 taaggcagaa ggccaggatc tgattggggg aaccattaac caaggacaaa ccatagatat
    53221 gttggttact attgaaaatg acgatactct ctttgccaag attattaatc tggtggaatc
    53281 tgcccaagaa aagaaaagca aaactgccac ctttatcgaa agcttagaag atggttatgt
    53341 caaatttgtg ctcgtcctta ttcctgcctt tatcctcttt agccactttg tgctttcttg
    53401 gacttggttg gctgcttttt accgagggat gattctctta acagtagctt caccatgtgc
    53461 cctaattgcc agttccacac ctgctagctt ggctgctatt tctcgtgcag ccaggaaggg
    53521 attgattatc aaaggagggg atattgtcga taacatggga gatattaagg ctgttgtcat
    53581 ggataaaacg ggaacgctca cccaaggaaa accttctgtt gtgaatgctc attatttgga
    53641 agatgagttg ctcgtgaata gactggtaaa aggagcagag gctgctagta cccatcctat
    53701 ttctaaagcc tttcttgaat acactgaaaa attggagcca ctgacctttg accacttaga
    53761 agaaatttct gggaaaggtt ttcagggctt ctatcaaggg caagaatggc gaattggcaa
    53821 gaaaacattc attttggaaa aggttcaaga cctatcagct tttgaagaaa ctattcaagt
    53881 ggaagaaaat caagggaaaa ccctaatctt tgtttcacgt gaccatcaat tgatagctta
    53941 ctataccctc ttggatgata tcaaaataga atcaaaacgt gctattaagt ctcttcatga
    54001 catgggaatc aaaacagtca tgttaacagg tgaccaagaa cgaaccgcca attatgtggc
    54061 acaaaaactt ggtattgatg aagtggtagc caactgtatg cctcaagata aggtggctaa
    54121 gttagcagaa ttaaagacca aatatggttt tgtggccatg gtaggagatg gtattaatga
    54181 tgctcctgcc cttgctcaag cagatgtttc ttatgctatt ggatcaggaa cagatattgc
    54241 aatggaaagt gcagacagtg tgattatgga tgacttgact cgtattccat tttcgattca
    54301 actttcccgc aaaatgaaga ccattatcaa acaaaatatt gtttttgcct tatctgtgat
    54361 taccttattg attttagcca atgtttttca ggtagttaac ttgccgcttg gtgttgttgg
    54421 acacgaaggc tcaacgattt tagtgatttt aaatggcttg cgtttacttt cttttaaata
    54481 atttagaaac ttgcttgaaa acttgaaagt gtttataata atagtaagac ttatgaaaga
    54541 gaaaaccgcc gtatgtttac tattatgatc attggtttag ctgtattttc aataattaaa
    54601 atcattctat ttttctattt ttatcagcaa tttgagcgca agcaaaaaag aaaagttgaa
    54661 tcaaacaacc agcaaagaca gtcccaagaa agtgacgctg tcccttcaaa aaaattccca
    54721 ttttaaaaag cttggaaagt gtacaaagtg cacatttcca agcttttttg actattaagg
    54781 ttaatctttg taacgtaagt tcccacgaaa atcgtctaag ctttggtagc ctttttctac
    54841 cataatcgtt tttagttcct tagtgacccg ttcaaagata gctggccctt cttgatgaag
    54901 ggctgttcca atttggacca tgcttgcgcc acaaagaata tgttcaaagg catcgcgtcc
    54961 tgttttaact ccccctgtac caataatgtg gatagaaggt tttaagcgtt tgtaaaacgc
    55021 atggacattg gctagagcag taggtttgat gtaatctcca cctataccac caaagccatt
    55081 tttaggcttg atgaggactt gctcatcctc aatgaccagc ccatttccga tagaattgac
    55141 acagttcaca aagaaaaggg ggtatttatt gaaaatcgca gccgcttgat caaaatgcac
    55201 aatgtcaaaa taaggaggta atttgatacc aagaggttta gtgtaatagg taaagatgtt
    55261 ctctagaagt tgatcagtgg tttcaaaatc ataagcaatc tgtggttttc ctggaacgtt
    55321 cgggcaagaa aggtttagtt ccactagacc ctcataatct gaagccatga tggcttttaa
    55381 aatggtttcg gtttcagtag gagatagccc gacaacggag agaaaatgag gtttatgttg
    55441 cccggttttg gcaagatcac tgacaaagtc taagtagtag cgaaaaccat tattagggag
    55501 tcccattgaa ttgatagaac caagacgagt atctgcgtag cgaggttctg gattacctgg
    55561 acgaacttca agcgtccctg ttttggtgac aaaggaagcg gcttgagact tttcaacctc
    55621 cataagctct tcttttgtca tgcagtaaac accagcagca ttcatcaaac agttatcaaa
    55681 agagaaatga ctaatttgtg tagcagtaga aaccattatt tcctccatat tttagacaaa
    55741 ttttttctat tctaccacta ggcatgaata atagcaagct atttttacaa aaatgtaaat
    55801 atattactgt tattgacgaa catttgaaat tttcatgata gttacattat gattttcaat
    55861 aaagtcagaa tagactagct tttttgctat attttttgta aaataggaat tgtaaaaagg
    55921 tagcaacctt taaattaaaa ggagatatta actcatggta aaattggttt tcgctcgcca
    55981 cggtgagtca gaatggaaca aagctaacct cttcactggt tgggcagatg ttgatctttc
    56041 agaaaaaggg acacaacaag cgattgatgc aggtaaattg atcaaagaag caggtattga
    56101 atttgacctt gctttcactt cagtattgac acgtgctatc aaaacaacta accttgccct
    56161 tgaaaatgca ggtcaattgt gggttccaac tgaaaaatca tggcgcttga acgagcgtca
    56221 ctatggtgct ttgactggca aaaacaaagc tgaagctgca gaacaatttg gtgatgaaca
    56281 agttcatatc tggcgtcgtt cttacgacgt attgccgcca gctatggcta aagatgatga
    56341 gtattcagca cacaaagacc gtcgttatgc tgatcttgac ccagccctta ttccagatgc
    56401 tgaaaactta aaagtaactc ttgaacgcgc aatgccttac tgggaagaaa aaattgctcc
    56461 agctcttctt gacggtaaaa acgtctttgt tggcgcacat ggtaactcaa tccgcgctct
    56521 tgtgaaacac attaaaggtc tttcagatga cgaaatcatg gatgtggaaa ttccaaactt
    56581 cccaccactt gtttttgaat tagatgaaaa acttaacatt gttaaagaat actaccttgg
    56641 tggtgaataa cttaaaaacc agactttggt ctggtttttt gattctatat cctaagaaga
    56701 aagaaaaccc tgcttaaaaa tatgatatga tagaaataaa aaaggataga tagattatga
    56761 aagcagaaga tcgtcgtcaa aaaattattg agtgcttaaa tagtgaacaa aaagctgtat
    56821 ctgctactcg tttaggtaag ttattaggag tgagtcgcca agtaatcgtt ggagatatag
    56881 ctcttcttcg agctcagcag attgatatta tttcgactcc aaaaggttat attatgtcaa
    56941 cagcgcttta tagtcaccaa ttttgtgcac gtattgtgtg ccaacataat gtagaagaaa
    57001 ctaaaaaaga attagaaatt attttagctc atcaagggat tattacgacg gtagaggtag
    57061 aacatcctat ttacggtatg ataacagccc ctttaaatat taagacacac tctgatgtaa
    57121 ctaatttcat gtccaaactc agtcaatcta aagcagagtt attgtcttcg ttgacagaag
    57181 gactacatag tcacttaatt tcgtgtccta gtcaagaagc ctttttagct atcaagcatg
    57241 atttagaatt agcaggtatc ttatacaaag gaatataact acagtatatt atgccattga
    57301 tggttgttat gaataccatg ctaaaatatt ctattgatta tttatcagca acaaggtatc
    57361 gtttaaaaat accaaaagat acaggtgtct tgatagttat tggtttttaa ggtatactat
    57421 ttttgaaaag gagataaaat gactaaaaaa ccaacacaac taatagctta tacctctatt
    57481 ttggtcgcat ttgcgatttt gattccgata atcatgcctc ttaagcttat tattggacca
    57541 gcttccttta ctctggcgag ccatgttcct ttgtttttgg ctatttttat gtcgattcca
    57601 gtagcaattt tggtcgctct tggtaccaca ttaggttttt tattagctgg acttccttta
    57661 attattgttt taagagcact ctctcattta ttgtttgcta ttttggcagc gtggtggtta
    57721 agtagaaaac cacaactgat gacatcggca gtaaaatgtt ttagttttgc gtttttcatt
    57781 aatgtcattc atggcttggc tgaattttta gttgtctata ttttaacagc aaccacagca
    57841 acttctatgt cttatttttg gtctatgcta ggcttgattg gcttaggttc gcttattcat
    57901 ggcatattgg atttctatct tgctttagtt ctatggcgtt ttttagcaaa aaacttaaaa
    57961 cttcccacca taaatgccta agactaaaat agagatagtt atatgataat gacgagccct
    58021 ttcttgttga aagggcttgc attttttagg aaaattgata tactaaagtg tgtgtttgtg
    58081 aatacagtaa caaaaggaga tatcatgaaa acaccagaac agattttaga agcaactatt
    58141 catattgggg aacataaggt taccaaaact tttttggcta aatctatctt aggctttatt
    58201 ggtggagcga tgattagcct tggttattta ctctacgtga gaattgccgc cagtggttta
    58261 gaaacttttg gtgctttttc cagtatcgta ggagcttgtg cttttccgat tggtctaatt
    58321 attattttaa tggcgggtgg agaattaatt acagggaaca tgatggctgt ttctgcagct
    58381 ttgttggcca aaaaaattaa gtttagcgag cttgccaaga attggcttat cattaccttg
    58441 tttaatgtga ttggagctgt ttttgtagct tttgtgtttg gtcattttct aggtttgaca
    58501 tcagctggta tttttaaaga agaagtgatt gaggtggccc atgctaaaat agcagcgagc
    58561 cctttgcagg cactagtatc aggaattggt tgtaactggt ttgttggttt ggccttgtgg
    58621 ctatgctatg gggcaaatga tgctgctggt aaattccttg gtacttggtt tcctgtcatg
    58681 acctttgtag cccttggttt tcagcatagt gtagcaaatg cttttgtcat tcctgctgct
    58741 atttttgaag gaggagcaac atggttggat tttgccacta attttatttt tgtctatagt
    58801 ggtaatatta tcggtggagc catttttgtt agttttttat attttaaagt gtactatcat
    58861 ccgcaaaaga gtaaaactca atgagaaaat atttttttag actgagggcg tcctattctc
    58921 agtcttcttt ttataattta tggtaaaata agaagcgaag tttaaaaaat ttagctaaat
    58981 aatggttagt ttattcccta aaaaaggagt attcccaaaa aatccgtaaa aagaatttgc
    59041 tatgggagta gttcccaaaa taatgcagat cattagttgt ctttcaaaaa tagccttgat
    59101 agggtaaaaa gtggacagtg atgcagcaac agattcatca ggcgtacaaa ctatcttatg
    59161 gtttgtgctt agagatttct taataatgct tttggtaata cacttttagt gaagaaagtg
    59221 tagcatgaaa gtttaaagat aaccatgagt ttgctttatg cactattgtt gagaaaatag
    59281 catagtcaat tgagtaaaca tcatttctgc atggaatgaa acaattccta taaacttata
    59341 tcataaggat ggtttgcggt ttctaaaaaa gtaacatgaa tcaaaaaaca aaagccaaat
    59401 atcccaaggc caaggtaaca agtcgagtaa aaaagttttt acttggctaa tccaaacggt
    59461 tctgtcacga ttcacgctaa aattggaact gctgagactt gtatcacaga caattcagat
    59521 cagaccttag taacttacaa cttaaatgta gtagcttata atacaaataa cgaagataat
    59581 attgcggttg ctgttatgta tccacatatt acgaatatat cggcaaagat gtgagggcgc
    59641 tttatgtggc gatatatgga aaacaataaa ggagaactta gtgctttatc caacacccat
    59701 tgcaaagtta attgacagtt actctaaact tccaggaatt ggtatcaaga cggcgacgag
    59761 attagccttt tatactattg gaatgtcaaa tgaagatgtc aatgattttg ctaaaaactt
    59821 attagcagct aaaagagaac tgacctattg ttcgatttgt ggaaacctta ccgatgacga
    59881 tccttgtcac atttgcacag acacgagtcg tgatcagacg accattctgg tagtagaaga
    59941 tgctaaagat gtttctgcca tggaaaaaat ccaagagtat catggctatt atcatgtgct
    60001 tcacggcttg atttcgccta tgaatggtgt ggggccagat gacatcaacc ttaaaagttt
    60061 aattacccgt ctaatggatg gtaaggtgag cgaagttatc gtagctacca atgccacagc
    60121 agatggggaa gcaacgtcca tgtatatttc acgtgtcttg aaaccagccg ggattaaggt
    60181 aactcgtttg gcaagaggtc tcgcagtagg ttcagatatt gagtatgctg atgaagtgac
    60241 attattgaga gctattgaga atcgtactga actttaatga tcatgttggt gcctttactc
    60301 gcaaagagag gttttgaaaa ggctacatct ttttgttata ataaaaaaac attcaattca
    60361 gataagaaag gttagctatg tccaaacaaa cgttagttct attatacgga ggccgctcag
    60421 cagaacgtga agtctcagtg ctttcagcag agagtgtgat gcgagcagtc aactatgata
    60481 agttcttagt aaaaacttat tttattactc agatgggcca atttatcaaa actcagcaat
    60541 tttcagaaaa gccatcggaa tcagaacgct tgatgacaaa tgagacgata gagctaactc
    60601 aaaaaataaa accaagtgat atctatgaag aaggtgcagt tgtatttcct gtattacatg
    60661 gacctatggg agaagatggc tctatccaag gtttcttaga agtgcttaga atgccatata
    60721 tagggactaa tgtcatgtct tctagcatag ctatggataa aattactact aaacgtgtct
    60781 tagaatcaat tggaattcca caagttgcct atactgttta tattgacgga caagatttgg
    60841 aggcttgttt agttgaaact ttagcaagac ttacttttcc tatcttcgta aaaccagcta
    60901 atatgggttc ttctgtgggg atttccaaag cccaaacaaa agtagaatta agaaaagcga
    60961 ttcagttagc cctgacctat gacagtcgtg tcttgattga acaaggcgtg gttgccagag
    61021 aaattgaagt tggtctcctt ggaaatgata aggttaaatc aactctacca ggagaagtaa
    61081 tcaaggacgt tgatttttac gactaccaag caaaatatgt tgacaataag attacgatgg
    61141 ccattccagc agatgtagac cagagtattg tgacagaaat gcgttcttat gctgaagtag
    61201 cctttaaagc cttaggtggt tgtgggttgt cccgttgtga ctttttcttg acccaagacg
    61261 gacaggttta ccttaacgaa ttaaacacca tgccaggctt tacccaatgg tctatgtatc
    61321 cactcttatg ggaaaatatg ggattggctt atccagattt gattgaagag ctggtgacac
    61381 tggcacaaga aatgtttgac cagcgtgaga gccacctgat ttaaacctat gagatgaata
    61441 aacattgact tggatggaac ggcaaacatc gtcaagatgt gacgttcgtc ctttttttgt
    61501 tgctctttat agtagagcca ttaagggagt ttgacatttt agaaattttt ctaatgtttt
    61561 gtgctataat agataggaca tcgtaaatac tagtgttctt agaaagaaaa caaaatgaaa
    61621 ttaaccttac acgaagtggc taaaatagtt gacgcccaaa ataatgtgtc tgacttagac
    61681 gacgttcctc tgcatcacat tgaatttgat agccgaaaga ttacaaaagg agacctcttt
    61741 ttacccttaa aaggtcaaag ggatggtcat gagtttattg atttagcctt tcaaaatgga
    61801 gctgtcgcta ctttttcaga aaaagaattg ccggggaaac ctcaccttct tgttgaagac
    61861 tgcctaaaag cttttcaaaa gctagcccat tattatattg ataagatgcg cgtggatgtt
    61921 attgctgtga caggctcaaa tggtaaaact tccaccaaag atatgatagg agctgtccta
    61981 tcaaccactt acaaaactta caaaacgcaa ggaaattaca ataatgagat tggtttgcct
    62041 tacaccgttc tccacatgcc tgacgatacg gaaaaaattg ttcttgaaat gggacaagat
    62101 cacatgggag atatttgtct tctgtctgaa attgcaaggc ctcgtattgc agttttaacc
    62161 ttagtcggcg aagctcattt agaatatttt ggaagccgag acaaaatagc ccaaggcaaa
    62221 atgcagattg ttgacggcat gaattcagat gggattttaa ttgctccggg agatccgatt
    62281 attgacccat atcttccaga aaatcagatg gttatccgtt ttggaaatca gcaggaaatt
    62341 gacgttactg gtattcaaga agacaaagat agcttaacgt ttacaacaaa tgtgttagca
    62401 acacctgtgt ccctacctct accaggaaaa tacaacgcga ccaatgctat ggtagcagcc
    62461 tatgtaggaa aactattggc agtaacagac gaggatatta tagctgcttt acagactgtg
    62521 acgttgacag gtaatcgtac ggagtggaaa aaagcagcca atggtgctga tattttatca
    62581 gatgtctaca atgccaaccc aacagccatg cgtctgattt tagaaacatt tgctaacata
    62641 gcaaagaacc ccggaggaaa aaagattgca gtgctagcag atatgaaaga acttggaaag
    62701 gattcagtga tacttcattc tcagttaatt gacagcttaa catcaggtaa tattgatcag
    62761 cttgtttttt atggggatca tattaaagaa ttagcacggt tggcaagtca ggtttatcct
    62821 gctgagcaag tacattattt tttaaaaact gaacaagaag accagtttga agccatggca
    62881 caatatgttc aaaacattct taatcccttt gatcaaattc ttttaaaagg cagccactcg
    62941 atgtcgctag aaaagttagt agatcgatta gtgactgaca agaattgtga cagctatcag
    63001 ccttgatttt tataaaactt tcagagaagg gcttgttctc tcatgaagat taaggtataa
    63061 tgaaaaaata tagaaaggga cgattatgga tttttttgca attgacccca tcggactgcc
    63121 tcatatctca cttatctttt acttgttgtc actcttgata gcgcttttgc ttgttttttt
    63181 gacttttcaa gcatataggt taaaatctta tcgttatttt ttcttgtttt tacagttatc
    63241 tcaggtgatt ggcttgtaca catggtacgt gttaaggggg tttcctttag atgaagcttt
    63301 gccgttgtat cattgtcgca tcgctatgtt agctatcttt tttctacctg atcgtaacaa
    63361 gtttaagcaa ttatttatgg ttttggggat aggtggaaca ttccttgcac ttttatcgcc
    63421 agatctttat ccgtttagat tatggcatgt agcaaatgtc tcgttttatc ttggtcatta
    63481 tgctttgtta gtgaatggtt tgatttacct cttgcgtttt tacgatgcca gtcagttaag
    63541 actgctatca gtagtacgct atttagcaac tgttaacttt cttcttctgt tggtcagttt
    63601 agccacgaaa ggaaattatg gttttgtgat ggatattcca gtgattcata cgcgtcattt
    63661 gctacttaat tttgtgatcg ttacaagtgg cttgactttt atggttaaaa taaccgaata
    63721 tttttacctt aaatttggtg aagctcaaca gctggcattt gctttctcta aagaaaagta
    63781 ggtcgttgaa aaagcactta aaatcagcta taataagaag agtctgggat gatttccagg
    63841 ctcgattttt ttaaaaaaga cactcattga tagcccaaga taagttataa taccagtttt
    63901 gaagaacttt agaggctaat cgtatatgga gaaacccaat gtagcccaac tcttttgata
    63961 aacaaataat acctaacgaa aataactcag agaaaagctc tgccatttta ctatacaagg
    64021 aaaaaagaat gtcacttaca gaagaaatta aaaaacgccg tacttttgcc atcatctctc
    64081 acccggatgc tggtaagacg actattactg agcaactatt atattttggt ggtgaaatta
    64141 gagaagcagg gacagtaaaa gggaaaaaat caggtacgtt tgcaaagtcc gactggatgg
    64201 atattgaaaa gcaacgtggt atctctgtta cttcatctgt tatgcaattc gattacgcgg
    64261 gtaagcgtgt taatattttg gatacacctg gacatgagga tttctcggaa gatacatacc
    64321 gtaccttaat ggctgtggat gcggctgtca tggttgtaga ttcggctaaa ggtattgagg
    64381 cgcaaacgaa aaaacttttt gaagttgtta agcatcgtaa tattccggtt tttacgttta
    64441 ttaataaatt ggaccgtgat ggacgtgagc ctttagaact cttggaagaa ttagaagaag
    64501 ttttagggat tgcttcttat ccgatgaact ggccgattgg tatgggacgc gcctttgagg
    64561 gcttatatga tttacataat aaacgtttag aactctataa gggagacgag cgttttgcaa
    64621 gtattgaaga cggtgatcag ctgtttgcca ataatccctt ttatgaacaa gtcaaagaag
    64681 atattgaact tcttcaagaa gcaggtaatg acttttcaga acaagctatt cttgatggtg
    64741 acctaactcc agtctttttt ggctctgccc ttacgaactt tggagttcag acttttcttg
    64801 atactttctt ggagtttgcc ccagaacctc atggtcacaa gactacagaa ggtaatgttg
    64861 ttgacccctt aactaaagat ttttcaggtt ttgtctttaa aattcaagct aacatggacc
    64921 ctaaacaccg tgaccgtatt gcttttgtgc gtattgtttc tggtgaattt gagcgcggta
    64981 tgggagttaa tttgacacgc actagcaaag gcgctaagct ctccaatgtt actcaattta
    65041 tggcagaatc acgggaaaat gtgacgaatg ctgttgctgg ggatattatc ggggtttatg
    65101 atacaggaac ttatcaagtt ggggatacct taacagttgg gaaaaataaa tttgaatttg
    65161 aacccttacc aacttttacc ccagaaatct tcatgaaggt ttctccgaaa aatgttatga
    65221 agcagaaatc cttccacaaa ggtattgaac aattagtaca agaaggagca attcagcttt
    65281 ataagaatta tcagacaggc gaatacatgc tgggtgctgt gggtcaactg cagtttgaag
    65341 tctttaaaca tcgtatggaa ggtgaataca acgctgaagt tgtgatgaca ccaatgggta
    65401 aaaagactgt tcgctggatt tcagaagatg atttggatca acgcatgtcc tcaagccgaa
    65461 acattttagc taaggatcgt tttgaccaac cagttttcct atttgaaaat gattttgccc
    65521 ttcgctggtt tgcagacaaa tacccagatg tcactttaga agagaaaatg taaccgaaac
    65581 atgattttca acagtctaac gttttggaga tcaatttact gagtaacttt ttaaaagaaa
    65641 tgagtggcta gttatctctt ttgcagattt aagattctat caaaaaacga tatcttcaca
    65701 actgtaagca tatttatgat ttagcggata atcgtgaaag cgtgttattg cttatattag
    65761 ccttatttcc tatctttttg attctaattg tgttacacta gttaggttgt taatcaaacg
    65821 acgaaaatta aaaggtttat aaggcagctt cgcactgcct ttttagaaaa gagaaacaat
    65881 tgaaatttac agaatttaac ttatcacaag acatccaatc agctgttgtt accgctggtt
    65941 ttgagaaagc atctcctatc caggaaatga cgattcctct cgcactagaa gggaaggatg
    66001 ttattggtca agcacaaaca ggaactggta aaacagcagc gtttggtcta ccaaccttga
    66061 ataagattcg tactaacgaa aatatcatcc aagcattggt tattgcacca acgcgtgaat
    66121 tggctgtaca aagtcaggaa gaattgttcc gttttggacg tgaaaaaggg gttaaggttc
    66181 gctctgttta cggtggatca agtattgaaa aacaaatcaa agcccttaaa tcaggtgccc
    66241 acattgtcgt gggaacaccg ggtcgcttgt tagatttgat taagcgtaag gctcttattt
    66301 tagatcatgt ggaaacctta atccttgatg aagcagatga aatgcttaac atgggattct
    66361 tggaagacat tgaagctatt attagtcgtg tcccagcaga ccgtcaaacc ttgctcttct
    66421 ccgcaacgat gccggcacct attaaacaaa tcggggttaa attcatgaaa gaccctgaac
    66481 atgttcaaat taagaacaaa gaattaacaa acgtcaatgt tgatcaatac tatgttcgtg
    66541 tgaaagaaca agaaaaattt gatactatga cccgtctgat ggatgttaac cagccagaat
    66601 tatccatcgt ttttgggcgg actaagcgcc gagttgatga aattacacgt ggcttgaaac
    66661 ttcgtggttt ccgtgccgaa ggaatccatg gtgatcttga ccaaaataaa cgcttacgtg
    66721 ttatccgtga tttcaaaaac gaccaaattg atattcttgt tgccactgat gtagcagcgc
    66781 gtggccttga tatttcagga gttacccacg tttataacta cgatattacc caagatccag
    66841 aaagttatgt tcaccgtatt ggtcgtacag gtcgtgccgg taaatctggt gagtctatca
    66901 cttttgtctc accaaatgag atgggctacc tctcaatgat tgagaactta acgaaaaaac
    66961 agatgaaacc tcttcgccca gcaactgctg aagaggcctt tcaagccaag aaaaaagtag
    67021 ccctcaagaa aattgaacgc gactttgcgg atgagactat tcgttcaaac tttgataaat
    67081 tcaaaggcga tgctgtccaa ttggctgccg aattcactcc tgaagaatta gctttatata
    67141 ttttgagctt aacagttcaa gatccagata gcttaccgga agttgaaatt gcacgtgaaa
    67201 aaccattacc attcaaatat gtgggtggtg gtcacggcaa taaaaatggc aaaggtggtc
    67261 gtggacgtga caaccgtaac cgtggtgatc gccgtggcgg ctatcgtggt gaccgtaaca
    67321 gagatgagcg tgatggtgac cgtcgtcgtc aaaaacgtga taagcgtgat ggtcacgatg
    67381 gcagtggcaa ccgcgatttc aaacgtaaat caaaacgtaa tagtaaagac tttttcaata
    67441 aagagaaaaa atcaagtgct aaaaatactg gttttgtgat tcgtcacaaa ggtgaataag
    67501 cttaaagaga gttgatgaga gtcagctctt tttaattttc tgaaaaagtt gcttttataa
    67561 tggaatctgt tataatggtt tcagttattt tgttataatt tttaaaaaat taggaggagt
    67621 ataagatgtc tgatagccat cttactgcct ttgacaaagc ttcaaaagca ggttttatta
    67681 ttgctttagg tattgtttat ggagatattg gaacaagccc cctttatacg atgcagtctc
    67741 tagttgaaaa tcaaggaggg gttaatcagg tttctgaatc ctttatttta ggatcaatct
    67801 ctttaattat ttggacactg accttaatca caacaatcaa gtacgttttg attgctctaa
    67861 aagcggataa ccaccatgaa ggtgggattt tttctttatt caccttggta cgtaaaatgt
    67921 caccgtggct cattattccg gcgatgattg gaggtgcaac acttttgtct gatggggctc
    67981 ttacacctgc ggtaacagtt acttcagcta ttgagggctt gaaagccgtt cctggtctga
    68041 gtcacatcta ccaaaatcaa accaatgtca ttatcacaac tctagtgatt ttaatagtct
    68101 tgtttggtat tcagcggttt ggaacaggtt ttattggtaa aatttttgga ccagttatgt
    68161 ttatctggtt tagttttcta ggtgttagtg gtttttttaa tatgttgggg catctggaaa
    68221 ttttcaaggc tatcaatcct tactatgctc tgcatttgct gtttagtcct gaaaaccatc
    68281 gtgggatttt tatcttgggg tccatttttc ttgcgacaac aggagctgaa gccctttatt
    68341 cggatttagg acatgttggt cgaggaaata tttatgtgag ctggcccttt gttaagatgt
    68401 gtattgtgct gtcttactgt ggccaggctg cgtggatttt agcgaataaa cactcaggta
    68461 ttgaactaaa ccctttcttt gctagtgtac cttcccaatt aacggtgtat gtggttattc
    68521 ttgccacctt agcagccatc attgcctcac aagccttaat ttcaggttct tttactttgg
    68581 tttcagaagc catgcgcttg aaaatattcc ctctatttcg agtgacttat cctggtgcta
    68641 atttggggca attgtatatt ccggtgatta actggattct ctttgcagtc acgtcctgta
    68701 cggttttgta ttttagaacc tctgctcata tggaagcagc ctatggcctg gccattacca
    68761 ttaccatgtt aatgaccact atattattga attactattt gattaaagaa ggtgtaaaac
    68821 ctttcctagc tcatttagtg atgactttct ttgcccttgt agaattcatt ttcttctggg
    68881 cctccgctgt caaattcatg cacggtggct atgtagtggt tattttagcg ctagcaattg
    68941 tctttgtcat gtttatttgg catgcgggca cacgcattgt tttcaagtat gtgaaatctc
    69001 tcaatttgaa tgattacaag gagcagatta agcaacttcg tgacgacgtt cgttttgacc
    69061 tttaccaaac caatgtggtt tatctctcca atcgcatgca ggatcacatg attgaccgtt
    69121 ctattttgta ttccatttta gataagcggc ccaagcgcgc tcaagtttat tggtttgtga
    69181 atgttcaggt aactgataag ccttatacag ccaagtataa ggtggatatg atgggaacag
    69241 attatatggt tcgagtcgaa ctttatctcg gttttagaat gccacagact gtgccacgct
    69301 atttgcgtac gattgttcat gacttgatgg aaagtggtcg tttgccaaag caagaacaag
    69361 aatataccat cacacctggc cgtgatgttg gtgattttcg ctttgtttta atagaagagc
    69421 gggtctccaa tgtacgtcaa ctcactaatg ttgaacgctt tattatgcag actaaagcga
    69481 gcatcaaaca tgtcactgcc agtccaatgc ggtggtttgg tcttcaatat tccgaagtaa
    69541 cccttgaagt ggttcccttg attctatcag atgtcttgaa gttaccaatc aaagaattgg
    69601 tgcctgtgga agacagtgaa gcataaaaaa atcattgaga tgataagtct caatgatttt
    69661 tcttttcttt ttgatgagta gcaatatagg ccaatttctg gcttcttgtt tttcgttttt
    69721 taaagagtgc ttcagcgctc atcgcagcct ctttgctatc aaacacttcg taataaagta
    69781 aagaaacagg taaccggtag cgagtatatt tagccccctt tcctgcgtta tgtgtagcta
    69841 agcgtttttt caaatcagtt gtataaccag tgtagagggt tttatctgca cattctaaca
    69901 catacatata agctttttta gtggtcatga ttaggttttc ctttggaagc agcaccaaaa
    69961 taaatctcaa aaatatgatc agtgtattct ccgttttcct tgtggacaac taaaggaggt
    70021 aaaatagtca tcccttcaat agaaccgtct ttgattgctt caataagtaa catattagct
    70081 gatttaccta atttaggata gacaaattgt acccttttag gggcaagacc attagctcta
    70141 aggctatcta taatttccaa gaatcgatct ggtcgatgaa ccattgccag acgaccattt
    70201 gatttaaggg catgacgggc tacctgacaa atctcttcta aattagtggt cacttcatga
    70261 cgagccagta aataatgttc agagacgttc ttcttagaac tttcgtgact tttaaagtaa
    70321 gggggattgc agagcatcaa atcaactcct gaacgtggca catggtttag gaggtttttt
    70381 aaatcatcac aaatcatggt tacttggtct tccagttgat tgagttggat agagcgttgt
    70441 cccatatcag caagccgttc ttgtaattct acttcaacga tggctgcctt ggttctagtg
    70501 ctagcaaata agccaaccgc cccatttcct gagcacaaat ccacaattaa ccctttggaa
    70561 ggcatcttgg ggaagcggga taagaggacg ctatcaatag agtaactaaa gacatccttg
    70621 ttttgaataa tgccaacatc acttgaaaag agttgatcaa tacgttcgcc ttcttttaag
    70681 attgctttta tcatacctgt tattttaaca taaaataggc ttgaggtgaa tgtttgaaga
    70741 gtgattttga ctttcgaaaa gtcaactttc atgatacaat aagtaagatt attctattaa
    70801 aggagaagcc tgtgttttac gcttacttac gcggtttagt agtcttttta ttatggattg
    70861 tcaatggcaa tgcccactac catcatgaag aaaagatgtt agatgcttct gagaattata
    70921 ttctggtagc accccatcga accttttggg atccagttta catggcattt gcagcacgtc
    70981 ctaagcagtt tatttttatg gctaaaaaag agttgtttgc aaatcgtctc tttgcttggt
    71041 ggattaaaat gtgtggtgct tttcctattg atcgtgacaa accgagtcca gatgctattc
    71101 gttaccctgt taatatgctc aaaaagagca atcgttctct tcttatgttt ccaagtggaa
    71161 gtaggcattc gcaagaggtc aaaggagggg tcgctgtgat tgctaaaatg gctaaggtca
    71221 agattatgcc agcagcctat caggggccta tgtcggttaa gggattacta gcgggagaac
    71281 gggttgacat gacttttggg aatcccattg acgtctcaga tattaaacgc atgaatgatg
    71341 aagggattgc tgaggtggca aaccgtattc aggcagagtt tgatcggatg gatgacgagt
    71401 tggcaccttt ccaaccaggc aaagcaagga accctttaac ttatctctac cgtttaccat
    71461 taggattggt tttggtggtg gtattattat taaccatgtt attttcctat attgctagtt
    71521 ttgtttggaa tccagataaa catcgccatt aagaacatga gggctaacat gtgtctgaaa
    71581 taagaagtga ttgagattag tcttctattg aaagaagccg ctaatgctta atcacttttt
    71641 tcatttctaa aaaagatatt cgatgatacc cttaactctt ttaagcacaa aaaatgacaa
    71701 tactactata cttttggata tcatttacga ataaaaagat gaggtaaaaa aatgttagac
    71761 gaattattaa ttaaaggaaa atcgtttttg agagagcggt attttctgcc atacctaatt
    71821 agtcttttat taggactttt tctaatacta agcttcagtt ttttgttctg gggaaatagg
    71881 cagagcaagg cagccgttcc tgctttgaga gaaatatcgc ctgtgaaaca gcaggttagt
    71941 gaagaaaaaa aggaaattca agaagatagc agtattttag tggatttaaa aggcgccgta
    72001 caaaaagagg gagtttataa gctaactgct agcagtcgtg tgcgagatgt cattgaacta
    72061 gctggaggcc taacttcaga agctgataaa catgctatta attttgcaga aaaactaact
    72121 gatgagcaag ttgtttatgt acctaaacaa ggagaagaga tttctgtgct accaagatca
    72181 ttagtttctg gtaaaaaaga aactgccagc aaggatcagt caaaggttca tattaataag
    72241 gccagcttag aagaactaca acatatccca ggtattggtg ctaaaagagc tcaggatatt
    72301 attgatatga gagataagtt aggtggtttt aaagcgttag aagatctccg tcaggtttcg
    72361 gggattggcg aaaaaacgct cgagaaatta aaagatgaca ttttcttgga ctaagctagt
    72421 gcccctgtca aagattcaat ttgctttttt aatcttggtt ttcttttatc aaatccatag
    72481 ccctagctgg ttaacgtttc tccttagcct tagtttgatt tgcttacttg tcaagcgttt
    72541 gtctaaaaaa gaatttctcg gtgtctttgc catccttagt ttctgtgctt tgtttttgct
    72601 gtatcagaaa cagcagttgg ttcaaaaact ggaaattcag ccagtacaaa taacctctgt
    72661 agcattagtt cctgatagta ttcggattaa tggtgatcaa ttagctgttc ttggacgtca
    72721 tgggaaacat tcttatcagt tattttaccg tttgaaaagc caagcagaag cccaattgtt
    72781 caagaaagag caccgttggt tagtgatgca cgcaaaagtg acattggaaa aagctgagga
    72841 agttcgaaat tttaaaggct ttaattatca gacttttttg gcttatcaag gaatttacag
    72901 gatagggaag gtagagcaaa tagaacagct tgaagtgatt tcacccgagt caatatgtga
    72961 ctatttgtcc tctttaagac ggcgagccat tgttcattgc cagcagcatt ttccaagacc
    73021 tatgtctcac tacctgacgg gcctcctttt tggttattta gataagtcgt ttggtgagat
    73081 gacagattat tatagtcagt tgggtatcat tcatctcttt gctttatcag ggatgcaggt
    73141 tggcttcttt ctcacttgtt ttagaagggt gttgctatta ctcgcagttc ctttggaatg
    73201 gataaaatgg atagagcttc cttttgcttc cttttatgcc gcgttgacag gttatagtat
    73261 ttctgttatt cgaagcctcg tgcagtcgca gttgcgtcat ttaggaataa aaggattaga
    73321 caatctggct tgtacttttt tacttgtctt tttatgggat gctcatttct taatgactgt
    73381 tggtggcgta ttaacgttta gctatgcttt tttgttaaca gtggttactg ttgaagaatt
    73441 atcaggagct aaaagacagt tggtacaagt cttgaccatt tccctaggaa tattaccatt
    73501 tttgctcttt tatttttcca gttttaaccc catgtcaatg gtcttaacag ggctcttgtc
    73561 ttatctcttt gaccttttta ttttgcccct tctttgtctg gttttttgtc tatccccttt
    73621 ggtgactgtc tcaatatgta accatctttt tattttatta gaaaaagtca ttcaatttct
    73681 cggaaatact ttcaatagtt cccttgtttt tggaagcccg accagttggc acttgcttat
    73741 cttggtgatt agcttcgcta ttttttatga ttatcggcag gtgcgacaac gagttatcac
    73801 ttgcggtttg gttatagcgt tgacgctttt gtctgtcaaa tatcctttga ccaatgaagt
    73861 gacctttatc gacattggcc agggagatag tattttggtg agggagtgga cagggaaaaa
    73921 tctcttaata gatgtaggtg ggcgcccttt cttttcttca aaagagcact ggcgacgaga
    73981 atatcacgta gctaatgctc agaaaacctt aattccttac ttaaaaagta gaggtattca
    74041 cacaattgac caactattag taactcacgc agatactgat catatggggg atatcgaagt
    74101 ggttgccaaa gctattcgga taaaggagat tttgactagt caaggcagtt taagccatcc
    74161 aatctttgtt aggcggctaa ggcgtttaaa atgtcacgtt agggttttag cagctggaga
    74221 ccagttgcct attatgggaa gtgtcttaca ggtactctac ccttggcagt taggggacgg
    74281 gaaaaataac gattctttgg tactttatgg caggctctta aatcgaactt ttcttttcac
    74341 aggtgacttg gaaaaagagg gggaaaacga gatcataaaa cgttatcctc aactaagagt
    74401 agattattta aaagcaggtc accatggatc aaatacctct tctagtgcag cttttttaga
    74461 ccatattcag cctaaagttg cctttatttc tgctggaaaa aataaccgtt atcagcatcc
    74521 tcacagagaa accttagccc gtttggaaga cagacaaatt acttattacc gaacggatac
    74581 acaaggagct attcgtttga ctggttggac aagctggcat cttgaaacgg tccgttagtt
    74641 aaaatttcag cctgacgaag aaacaagcag tcgctttcct tttaggagaa agatgatata
    74701 ataaaagcat gattgcgata gaaaagattg aaaaactgag taaagaaaat ttgggtctta
    74761 taacccttgt cacaggagat gacattggtc agtatagcca gttgaaatcc cgcttaatgg
    74821 agcagattgc ttttgataag gatgatttgg cctattctta ctttgatatg tctgaggccg
    74881 cttatcagga tgcagaaatg gatctagtga gcctaccctt ctttgctgag cagaaggtgg
    74941 tcatttttga ccatttgtta gatatcacga ccaataaaaa aagtttctta aaagaaaaag
    75001 acctaaaggc ctttgaagcc tatttagaaa atcccttaga gactactcga ctaattatct
    75061 ttgctccagg taaattggat agtaagagac ggcttgttaa gcttttgaaa cgtgatgccc
    75121 ttgttttaga agccaaccct ctgaaagaag cagagctaag aacttatttt caaaaataca
    75181 gttatcaact gggtttggga tttgagagtg gcgcttttga acaactgctt ttaaaatcaa
    75241 acgatgattt tagtcaaatc atgaaaaaca tggctttttt aaaagcctat aaaaaaacgg
    75301 gaaatattag cctaactgat attgagcaag ccattcctaa aagtttacaa gataatattt
    75361 ttgacttaac cagacttgtc ctaggaggta aagttgatgt ggctagagat ttgattcatg
    75421 atttacgctt atcaggggaa gatgatatta aattaattgc tattatgttg gggcaatttc
    75481 gcttatattt acagcttgcc attcttgcta aagaaggaaa atcagaacag cagcttgtaa
    75541 ccagtttgtc agagatactt ggtcggcggg ttaatcctta ccaggtcaag tatgcgttaa
    75601 aggattctag gaccttatct cttgcctttc taacaggagc ggtgaaaacc ttgattgaga
    75661 cagattacca gataaaaaca ggactttatg agaagagtta tctagttgat attgctctct
    75721 taaaaatcat gactcactct caaaaatagg cgttggaccg ctttccaaga gataatcaaa
    75781 ttagttgaaa gctcttattt tttgcgataa gatagacgta tcataaagga aaagaggaca
    75841 ggaaaatggc tattatttta ccagaacttc catacgcgta tgatgctctt gaaccacagt
    75901 ttgatgcgga gacaatgact cttcatcatg ataaacacca tgccacttat gtcgctaata
    75961 ccaatgcggc tttagaaaaa cacccagaga tcggagaaaa tctggaggaa ctattggcag
    76021 atgtcactaa gattccagaa gatattcgtc aagccttaat taataatggt ggcggacacc
    76081 taaaccatgc gcttttctgg gaactattat cacctgaaaa acaagacgtc actcctgatg
    76141 ttgcacaagc cattgatgat gcttttgggt catttgacgc ctttaaagaa caatttacag
    76201 cagctgctac tggacgtttt ggttcaggtt gggcatggtt agttgttaat aaagaaggac
    76261 aactcgaaat cacatcaacc gctaaccaag atactccaat ttcagaaggc aaaaaaccaa
    76321 ttctagcgct tgatgtgtgg gagcatgctt actacttgaa ttatcgtaat gtacgtccaa
    76381 attatatcaa agctttcttt gaaatcatta attggaaaaa agtatcagaa ctttaccaag
    76441 ccgctaaata agaaaagggt ttccaatctc aaaaagagat tggtttttta agtgcttttg
    76501 tgaataacag tgtggggaca tcatttttta gaaaagataa aaatagtttg tctttttaat
    76561 gtaattcctt gatttttagc taaatttagc tagaatagaa tggataggag gttttaggtg
    76621 ttaagagaga agaaaactac cattgtaggg attattttag gaatgatgat agtgacctta
    76681 cttgtcggct gtttgactgg taaaaagaaa ggctttcctc ctaaagcagg tgagcggcag
    76741 caaacggctg acttaaaaat gaaaagcaag attagaaaaa ctgttcaaaa accgttacct
    76801 gaaaaatcta aagaggctaa gcaagctgtt atggatgcag atttagcgta aatcgatgcc
    76861 ttagggctct attacgatga caaccatttg agtttggtca atacggtcaa ggcgtattta
    76921 aaggaatttg gcattggtga atcccaaatt gccttttcct acaaagacct caattcaggt
    76981 aggactgctg ccatgaatga ccatcagcca atgattgcag gaagcaccta taagctgcca
    77041 ttaaacatgc cggtggtaga taaggcaaat tatccatgac agagcgtttt gacattacag
    77101 aaactgatta tgaatatgat caggaacatc atgcctatgt tgctcaattc aatggtgcca
    77161 tgtctattcc ggatatgcaa gaatattcct tggtgtgctc agaaaacacg ccagcttatg
    77221 ctctagcaga acgattgggt ggcatgaata aggcttacca attatttgac cgctacggga
    77281 aggtatccgg ggctattacg accattgatc gtaatggcaa taaaatcacc accgcttact
    77341 atttgcaagt tttggattac ttgtggcagc atcaggataa atataaggat atcttgtact
    77401 acattggtga atctttccct gatttgtatt acaagactta tttgcctcat gtaaaggttt
    77461 atcaaaaacc gggttatgtc agagaagcct taaatgtagg tgccattgtt tgcgaagagt
    77521 ctccttattt gattgctctt tattcatctg gtttgggtgg agcaacacaa gcatcagagg
    77581 aagtcaatgg ccttggttat gtgcagcttg ttcagctaac atatgtaatt aatgagtggc
    77641 atagagtcaa tcacaacatg atctaatact cagttgacat tagtctgata gtagacatac
    77701 ttactgatga cagtcactaa aataagtttt tgctcattaa aaaagcttga cctgaagaaa
    77761 caggtcaagc tttttgagat taatgctgtg tttttgagat taatgctgtg tttttgagat
    77821 taatgctgtg tttttgaggt cttattgtca cctcttgttt cttgataatg gtgaatcaat
    77881 tttttgaatt ttttaacggc agcgcgtgtt agctgcttag atgatttgcc atttctgctg
    77941 taaatgggat tgtgcttatc cctttgctgt tctaaatcgg gaactagaaa agttgcatca
    78001 ggactattaa ttgctttttc aataaaagcc ggatcaaaaa gcagaatagg gtcaatatca
    78061 agcagacggt gcaagtcatc gttttggttg ccataattga aagaagaacc attgacaata
    78121 ctatttcgcg ttaggttatc aggatctagt gctactaaaa attgtccaga ttctgacaaa
    78181 ataaactctg agtgaaaatg aatcaaaatc ttatagttgg cttgaggaat attgtctgta
    78241 tagactgcct gatgattgcc attagcatct aaaatactag caaccttatt atggtatcta
    78301 gaagattcat aaagagcata atcgtcatct tctagagtag ccaaatattt ggctaaggca
    78361 tccgcatcgg ttttcccttt attcccataa tgtgagcgaa cccactccgt ttgctgggtt
    78421 gatatgaggt aacggagttg atgaattttc cgattaaaac ggctataagg aatgtgttcg
    78481 agattaacat aacaagcctg aatcagggca gataattggt cccaaaaagg atctgttggc
    78541 gatagctttg gacttaattg tgccaatact tgctgtgttt gttttgattg tcctgtcaac
    78601 tcgtctggca tctttaaata cgtcaagagc agctgtagcc gtttttgagc ttcttgaggc
    78661 gtctgtgcca tctcttcttg ttgccagagc ttttgaaagt caggtgatcc tatcaggcga
    78721 atgcgactat aaagttgttc gaaatgataa gagtttcctc taacagcgag ttgttcaagg
    78781 tatccttttt ggatggcttt ataggaccat ccgtccttat cgagttttcg aacataatca
    78841 agcaagtcgt ggcgtaattt aatagcttct gaaagctgga gctctcgtaa tttaggatag
    78901 ttttggtaaa aaggtgccac taaatcttga taaagtttgt gttctttttc aaaggcaatc
    78961 cgctgatagt ctttctgcca aaaactgacg caaaaagtca gaaacatcaa gaagaatagc
    79021 cattttccaa gtaatttcat aggtttttag ttgacaaaca tggcatcgcc aaagctaaag
    79081 aaacgatact tttcgtcaac cgcgtgtctg taagcttcaa ggacaaagtc acgccctgca
    79141 aaggctgata ctagcatcac aagggttgat tttggaagat ggaaattagt tgaaaaagca
    79201 tcaactacgt tgaattggta gcctggttta ataaaaatat tggtccaacc tgaatcagct
    79261 tgaatgtccc cttggaactt acttccgatg gtttccaacg tacgaataga ggtggttcct
    79321 acagcgacaa cacgaccgcc agcttgtttg acgtctctta gggtttgcgc tgcttcttct
    79381 gagagggaat aaaattccga atgcatatca tgctcatcga ggttatccac ggaaacaggt
    79441 ctaaaagtcc caagccctac atgtagggtc aaataaacca aatgtacccc tttggcttcg
    79501 atttttttga gcaagtctgt tgtgaagtgg agaccagcag ttggggcggc cgcagagcca
    79561 ttttctttag cataaacggt ttggtatcgc tcagcatcct ctaatttttc gtgaatataa
    79621 ggaggcaatg gcatttcgcc gaggctctct aaaacttcta agaaaatccc atcatagctg
    79681 aattcaacaa ttcgtccacc atgttctaat tcgtcgataa tggtggcttt gagacgccca
    79741 tctccaaaat tgacttggct accaaccttg agccgtttgg ctggcttggc taaaacttcc
    79801 cattgatctc cttgtgtatt tttaagcaaa agcaattcca catgaccatg agtatcgggt
    79861 ttttctccat aaagtctggc aggcaagaca cgggtgttat tcatgacgag ggcatcccct
    79921 ggatttaatt gatcaatgat atggtcaaaa tggctatcaa ccatagtttt ttgacggtga
    79981 tcaataatca atagttttga actgtctctt ttttcgagtg gggtttgggc aatcaattct
    80041 tctggtaact caaaatcaaa atcgttagta ttcattgggg ctcctttaaa gccactagta
    80101 aatctcttcc tagtaactct ttcatgtaag ataaatcttt tctattatat cacttgtcgt
    80161 ctaacccgtc atctttaatc tttcatcaga aagtaaagga taggcttgac aaaaattggt
    80221 atataccata taataaaaac acgaaaggtc tataccaatt agaaaaggag aatgacatga
    80281 aaattattcg ggtccaagat cagattgaag gtgggaaaat cgcttttacc ttattgaaag
    80341 atagcttagc taagggagct aagacgttag gattagcaac aggaagttca ccaattagtt
    80401 tttaccaaga aatggtcaaa agtcctttgg atttttcgga cttaacaagt attaacttgg
    80461 atgaatatgt cggtctttca gtagagagcg atcagagtta tgattatttt atgcgtcaaa
    80521 acctttttaa tgcgaagcct tttaaaaaga attacttgcc caatggttta gcaactgata
    80581 tcgaagctga agctaaacga tatgaccaaa tcattgcaga gcatccgatt gattttcaag
    80641 tgcttgggat tgggcgtaat gggcacattg gttttaacga acctgggacc tcttttgaag
    80701 aagaaacgca tgtggtggat cttcaagaat caaccattga agccaatagt cgtttcttta
    80761 caagtattga ggatgtgcca aaacaagcta tctctatggg aattgcctcc atcatgaagt
    80821 ccaaaatgat tgttcttctt gcctttggac aagaaaaagc tgatgcgatc aagggaatgg
    80881 tttttggacc aatcacagaa gacctcccag caagtattct ccaaaaacat gatcatgtta
    80941 tcgtcattgt ggatgaagct gctgcgtcac aattagacta acctaaccag tcgttaaacc
    81001 ttgtaagaac aaaaatgagg agatagtcaa cctcatttaa gaagaagtct tgtaacttct
    81061 atttcttttg caagggtaaa tttatgttac actactaata tgcgtttaga taaattatta
    81121 gaaggtacaa aagttggctc tagaagtcaa gtgaagaaac tgattaaggc tcaaggggtt
    81181 tgggttgatc acatgcctgc cagaaatggt cgacaaaatg ttgatcctgg cttacaactg
    81241 attgaggtga ctggacagcg agtgacgcat cctaaacatt cttacattat cctcaacaag
    81301 ccatcagggg tggtctcagc taagaaagat actaactatt taaccgttat tgatcagctt
    81361 gctgaagaag ataaaagtcc agacttgtat cctgttggca gattggatag agataccgaa
    81421 gggttggttt tactcactga taatggtccc ctagggtttc ggatgttgca ccctagtcat
    81481 catgtttcta aaacgtatct tgtaaccgtt aatggcttgt tagcagaaga tgcctcagat
    81541 ttttttgcag ctggtatttg tttcccaaca ggtgaacaat gccaaccagc tcaattgact
    81601 attcttaaag ccgatacaga ccagtcacag gctagtttga ccatttcaga aggaaaattt
    81661 caccaagtga aaaaaatgtt tttgacctat ggtttgaaag tgacctctct gaaacggacg
    81721 cactttgcag ggctggaact cggtcattta gccagcggag aatatcgtta tctaacagcg
    81781 tcagaaaggc aactcattaa aacgtatctg gactgagcaa aacatgcttt caaaaagaag
    81841 ctattcttgc ttaatcaata aagtgtccat tgactattat agtcatgatg agtccgactt
    81901 ttgctatgtt tagtcatctt gttgttacaa aatcagataa gcaaaaagat tcaattccca
    81961 ctatctcctt aacctgaagc gttataaaaa cccttgtgtt aaactctcat aaaattggaa
    82021 atgggagtgt tttttgatgg ctaaaaaaac taaagctact taaagtttgg tacttagttt
    82081 tcgaataata aggtggaaga tctaatagga ggtgacaaaa atattaaccg ctttagatgg
    82141 taagaatcag ctcatttcgc tagtaactca gcctatttca acgaaaccgc cttttcgttg
    82201 cccagcttgc aaatctcctg ttcgcttgcg tcaggggaca attagacgac ctcattttgc
    82261 ccatgtacaa ctagctcatt gtcaattcca agcagaaaat gaatctgagg aacatttgac
    82321 gttgaaggca aaactttaca ccagtttagt ccgaacagaa gctgtctgta ttgaaaaata
    82381 tcttcctgag ctgcaacaga tagcagacct ttgggtgaat gataagttag ccttagaaat
    82441 tcagtgcagt cccttgccag ttgagcgtct aaagaaacgc accaaagcct atcaagaaaa
    82501 ggggtatccg gtacgatggt tactgggaag aaaattgtgg cttaacactc acttaactgc
    82561 tttgcaaaaa cagtttcttt acttttcatc ttgtttgagc tttcatttgt gggagctgga
    82621 tgcagctgca aatctgctac gtctcaaata tttaatccat gaagacttat ttggtaaggt
    82681 aagttaccta accaaaacca tttctttaga ccacaatatt atggaaatgt ttcgattacc
    82741 ctatcagcaa gaaatccttt attcttatca gaaaaaaatg actgtgaacc tatctaaaag
    82801 aatacaaaga gctcttttag ctagacaccc gaaatggcta cgacgacaag aaaaggctta
    82861 cttatcaggc tataatttac ttatgttaac aactgatgct ttttatcctc aatggagacc
    82921 agttcaatct tccagcggtt tttgtcaaat taaaggaaat cttcgccctt attatgaaag
    82981 ttttaaagtc tattataaaa aagaaaagga taagaaggtg caaacgcttt tttcaccaaa
    83041 atattatgtt aaaatggata gtaatagaaa atgagaagga gatgttatga ctgataatcg
    83101 cagccattta gaagaaaaat acacttggga tttgagcact atttttgcaa cagataagga
    83161 ttgggaagct gaagtcagtg acttagctac tgaaattgac gcggctaagg atttttcagg
    83221 tcatttacta gattctagcg ctaatctgtt gacaattaca gatacttatt tagagttagc
    83281 tcgtcgtgtt gagaaagtct atgtttatgc tcacatgaaa aatgaccaag acactactgt
    83341 tgccaagtat caagaatacc aagctaaggc ttcaggcttg tatgcaaagt ttagcgaagt
    83401 gttttcattc tatgacccag aagttatggc attgagtcag gaagattacc aagcttttct
    83461 tgcggaaaca cctgatttaa aaaggtatca tcatttcttt gacaaattgt tccagacacg
    83521 tgaccatgtg ctcagccagg ctgaagagga attattagca ggagcccaag aaattttcaa
    83581 cggcgctgaa gaaaccttta gtattcttga taacgctgat attgtctttc cagtagttaa
    83641 aaatgataag ggggaagatg ttgaattaac ccatggtaac ttcattagcc taatggaatc
    83701 taaggaccgt agcattcgcc aagctgctta tgaagccatg tatagtactt atgaacagtt
    83761 ccaacatact tacgctaaaa ccttgcaaac caatgtgaaa gtgcaaaact ataaggcgcg
    83821 tgttcataaa tacgattcag cacgtcaagc tgccatggct gctaacttta ttccagaagc
    83881 tgtttacgat accttgttag aaacggttaa taaacatttg ccactcttac accgttattt
    83941 gaaattgcgt caagaggttc ttggtttaga cgatttgaag atgtatgatg tttacacacc
    84001 attatctgaa acagatttag ccattggtta tgatgaggct ttggaaaagg ctgagaaggt
    84061 tttggctgtt tttggtaagg attacgcgga tcgtgtacat cgtgctttta ccgaacgttg
    84121 gattgatgtt cacgtcaata aagggaagcg ctctggggct tattcaggtg gttcctatga
    84181 taccaatgcc ttcatgctgt tgaactggca agatacactt gataatcttt acacacttgt
    84241 ccacgaaaca ggccacagtt tgcattctac tttcactcgt gaaacacagc cttatgtgta
    84301 tggggattat agtatcttct tggcagaaat tgcatccaca acgaatgaaa acatcatgac
    84361 agaagctctc ttaaatgaag tgcaagatga gaaagaacgc tttgccattt taaaccatta
    84421 cctcgatggt ttccgtggaa cagttttccg tcaaacccaa tttgctgagt ttgaacatgc
    84481 cattcatcaa gctgatcaaa aaggagaagt gttaacaagt gaatacctta accagctata
    84541 cgctgattta aatgaaaaat attatggttt aagtaaaaaa gataatcatt ttattcagta
    84601 tgaatgggca cgtattcctc atttctatta caattattat gtttaccaat atgcaacagg
    84661 gtttgcagct gcaagctatt tagcggataa gattgtgcat ggcacacaag atgatatcga
    84721 tcattacttg gcctacctca agtcaggaaa ttctgattac ccacttgaag tgattgctaa
    84781 agcaggcgtt gatatggcaa aaggtgatta tttggaagct gcctttaaag tctttgacga
    84841 acgtctaaca gaacttgaag tactcgtgtc caaaggcata cacaaaagct aaaatcacaa
    84901 aagtcaagga caaaaagagt ttaggattga tagaaatgga gattcacagt tggtgtgaat
    84961 ctccatttct atcggtcatt ttctttttat attttagtgg attatcagtc ctgaaaagca
    85021 ggcttcattg accttagttt gacggtttct tttttgtctt ttttattgtg tggtcactca
    85081 cttaaataaa accaatagtt gagtaaggcg cttcagatta acgccattcc attgttttag
    85141 agataacgtt cgtataaaac atgtttttat tatattt
//
LOCUS       DAUGFP010000002        80301 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.002, whole genome shotgun sequence.
ACCESSION   DAUGFP010000002 DAUGFP010000000
VERSION     DAUGFP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 80301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80301
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.002"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            30..2447
                     /gene="hylA"
                     /locus_tag="VH663_000080"
     CDS             30..2447
                     /gene="hylA"
                     /locus_tag="VH663_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEQ8173318.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQTPTTTDSEKKVVQSQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASANITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTTPIEKFVPDPTRFRVRAANFSPFEANSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSPIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     SDTFVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYCSYVNNQPVDLNNQLVDFTNTKSIFLESDDPAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYEKKATTFSNHSLSHQGFYSFPH
                     PVKQNQQ"
     gene            complement(3122..4885)
                     /gene="lpdA"
                     /locus_tag="VH663_000081"
     CDS             complement(3122..4885)
                     /gene="lpdA"
                     /locus_tag="VH663_000081"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEQ8173319.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAM
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            complement(5212..6621)
                     /locus_tag="VH663_000082"
     CDS             complement(5212..6621)
                     /locus_tag="VH663_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEQ8173320.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPTVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKVAAEMGIDLGQVPGTGPKG
                     RVHKEDVENFKGAQPKASPLACKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLPEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            complement(6806..7804)
                     /locus_tag="VH663_000083"
     CDS             complement(6806..7804)
                     /locus_tag="VH663_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEQ8173321.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            complement(7863..8831)
                     /locus_tag="VH663_000084"
     CDS             complement(7863..8831)
                     /locus_tag="VH663_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEQ8173322.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
     gene            complement(9116..11023)
                     /locus_tag="VH663_000085"
     CDS             complement(9116..11023)
                     /locus_tag="VH663_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ8173323.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDQTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMTDYDLLTEHFRQQGGFTYESDIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFMTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLTVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVEIG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQEALME
                     EWETIAEQIES"
     gene            complement(11069..11395)
                     /locus_tag="VH663_000086"
     CDS             complement(11069..11395)
                     /locus_tag="VH663_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173324.1"
                     /translation="MKQLLAIILWLPKLIVKLFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            complement(11425..12210)
                     /locus_tag="VH663_000087"
     CDS             complement(11425..12210)
                     /locus_tag="VH663_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEQ8173325.1"
                     /translation="MANIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPKTLLEGN
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFAANEKAIADF
                     RAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            complement(12343..14004)
                     /locus_tag="VH663_000088"
     CDS             complement(12343..14004)
                     /locus_tag="VH663_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ8173326.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKDKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            complement(14208..14966)
                     /locus_tag="VH663_000089"
     CDS             complement(14208..14966)
                     /locus_tag="VH663_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173327.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            complement(14963..15832)
                     /locus_tag="VH663_000090"
     CDS             complement(14963..15832)
                     /locus_tag="VH663_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173328.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCLPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERFFKGVRLTR"
     gene            complement(16177..17175)
                     /locus_tag="VH663_000091"
     CDS             complement(16177..17175)
                     /locus_tag="VH663_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ8173329.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANRQNITIGIL
                     QFVTHEALDDIKRGIEDQLKKQMPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAAASKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKKLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVVDVPVKVVDIGQPLINKNVAAELGIAIKREDFPSASFIEN"
     gene            17529..19181
                     /gene="fbp54"
                     /locus_tag="VH663_000092"
     CDS             17529..19181
                     /gene="fbp54"
                     /locus_tag="VH663_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEQ8173330.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRVQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSEMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLS"
     gene            complement(19240..20487)
                     /locus_tag="VH663_000093"
     CDS             complement(19240..20487)
                     /locus_tag="VH663_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEQ8173331.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDLEGLTEVAREKLLQAVAISSEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLMYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(20477..21658)
                     /locus_tag="VH663_000094"
     CDS             complement(20477..21658)
                     /locus_tag="VH663_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ8173332.1"
                     /translation="MPLIPDKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINRLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            complement(21716..22192)
                     /locus_tag="VH663_000095"
     CDS             complement(21716..22192)
                     /locus_tag="VH663_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEQ8173333.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            complement(22413..23453)
                     /gene="rfbB"
                     /locus_tag="VH663_000096"
     CDS             complement(22413..23453)
                     /gene="rfbB"
                     /locus_tag="VH663_000096"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEQ8173334.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKTDAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(23697..24290)
                     /locus_tag="VH663_000097"
     CDS             complement(23697..24290)
                     /locus_tag="VH663_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEQ8173335.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYALVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            complement(24290..25159)
                     /gene="rfbA"
                     /locus_tag="VH663_000098"
     CDS             complement(24290..25159)
                     /gene="rfbA"
                     /locus_tag="VH663_000098"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEQ8173336.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHKLAQSLKKN
                     EYGQYLLRLIGEA"
     gene            complement(25217..26323)
                     /locus_tag="VH663_000099"
     CDS             complement(25217..26323)
                     /locus_tag="VH663_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEQ8173337.1"
                     /translation="MKIAIVGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGNDFKGLIGFDNCLYASGAARVEGAALCETLL
                     KASGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLRPLGYQVDVRPQKGQ
                     LLDYDVHHIISDTYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKTCRVGIRAYTSDYSPFYGQVPGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLWSAGKA"
     gene            complement(26363..27151)
                     /locus_tag="VH663_000100"
     CDS             complement(26363..27151)
                     /locus_tag="VH663_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEQ8173338.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMEGDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISIYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            complement(27141..27827)
                     /locus_tag="VH663_000101"
     CDS             complement(27141..27827)
                     /locus_tag="VH663_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEQ8173339.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIA
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            complement(27899..28555)
                     /gene="nth"
                     /locus_tag="VH663_000102"
     CDS             complement(27899..28555)
                     /gene="nth"
                     /locus_tag="VH663_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEQ8173340.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            complement(28552..29235)
                     /locus_tag="VH663_000103"
     CDS             complement(28552..29235)
                     /locus_tag="VH663_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ8173341.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFHLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            complement(29316..29834)
                     /locus_tag="VH663_000104"
     CDS             complement(29316..29834)
                     /locus_tag="VH663_000104"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEQ8173342.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            complement(29985..32195)
                     /gene="recJ"
                     /locus_tag="VH663_000105"
     CDS             complement(29985..32195)
                     /gene="recJ"
                     /locus_tag="VH663_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEQ8173343.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYDRGIRTE
                     EALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DERLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKAWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            complement(32192..32956)
                     /locus_tag="VH663_000106"
     CDS             complement(32192..32956)
                     /locus_tag="VH663_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEQ8173344.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKIVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAKEIADM
                     FQVNTLASIHFACLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFNQADPSGHYLESVGKFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            complement(32956..33885)
                     /locus_tag="VH663_000107"
     CDS             complement(32956..33885)
                     /locus_tag="VH663_000107"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEQ8173345.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(33900..34535)
                     /locus_tag="VH663_000108"
     CDS             complement(33900..34535)
                     /locus_tag="VH663_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEQ8173346.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(34528..35766)
                     /gene="hflX"
                     /locus_tag="VH663_000109"
     CDS             complement(34528..35766)
                     /gene="hflX"
                     /locus_tag="VH663_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEQ8173347.1"
                     /translation="MVETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(35839..36738)
                     /gene="miaA"
                     /locus_tag="VH663_000110"
     CDS             complement(35839..36738)
                     /gene="miaA"
                     /locus_tag="VH663_000110"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEQ8173348.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLIDIREVTESYSAYEFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGTVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDHMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            36872..37048
                     /locus_tag="VH663_000111"
     CDS             36872..37048
                     /locus_tag="VH663_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEQ8173349.1"
                     /translation="MVKKYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(37166..38074)
                     /locus_tag="VH663_000112"
     CDS             complement(37166..38074)
                     /locus_tag="VH663_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEQ8173350.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFNYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FWGNLVLIAYYQYRFVFSFSWDNVSPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
     gene            complement(38311..38730)
                     /locus_tag="VH663_000113"
     CDS             complement(38311..38730)
                     /locus_tag="VH663_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEQ8173351.1"
                     /translation="MPFIAKQTLKSQLIPQDNLLADSRFNEIMDYLTGDFPFVFRPMF
                     NPHRYTISQDNQALEKVKQASYKRMGIAMTHLDGLIGESGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLAAFMAKIVEDSAVQQVLNAAH"
     gene            complement(39041..40027)
                     /locus_tag="VH663_000114"
     CDS             complement(39041..40027)
                     /locus_tag="VH663_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEQ8173352.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPEFVGV"
     gene            40223..40525
                     /locus_tag="VH663_000115"
     CDS             40223..40525
                     /locus_tag="VH663_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173353.1"
                     /translation="MTYELCLEYGTYPLSRVDAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVATILKCKYKDYPIKIETFLL"
     gene            complement(40888..40959)
                     /locus_tag="VH663_000116"
     tRNA            complement(40888..40959)
                     /locus_tag="VH663_000116"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40924..40926),aa:Arg,seq:ccg)
     gene            complement(41072..42277)
                     /gene="rpsA"
                     /locus_tag="VH663_000117"
     CDS             complement(41072..42277)
                     /gene="rpsA"
                     /locus_tag="VH663_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEQ8173354.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
     gene            complement(42404..42475)
                     /locus_tag="VH663_000118"
     tRNA            complement(42404..42475)
                     /locus_tag="VH663_000118"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42441..42443),aa:Gln,seq:ttg)
     gene            complement(42526..42606)
                     /locus_tag="VH663_000119"
     tRNA            complement(42526..42606)
                     /locus_tag="VH663_000119"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42570..42572),aa:Tyr,seq:gta)
     gene            complement(42667..42897)
                     /locus_tag="VH663_000120"
     CDS             complement(42667..42897)
                     /locus_tag="VH663_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEQ8173355.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
     gene            complement(42961..44028)
                     /locus_tag="VH663_000121"
     CDS             complement(42961..44028)
                     /locus_tag="VH663_000121"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEQ8173356.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVRLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            complement(44106..46565)
                     /gene="parC"
                     /locus_tag="VH663_000122"
     CDS             complement(44106..46565)
                     /gene="parC"
                     /locus_tag="VH663_000122"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEQ8173357.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKEADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAGKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            complement(46656..48605)
                     /gene="parE"
                     /locus_tag="VH663_000123"
     CDS             complement(46656..48605)
                     /gene="parE"
                     /locus_tag="VH663_000123"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEQ8173358.1"
                     /translation="MTKNEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGSVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVDSIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGANFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVETGHVYIALPPLYKMSKGKGKTEKIAYAWTDGEL
                     EDLRREFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTINDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            48740..49381
                     /gene="plsY"
                     /locus_tag="VH663_000124"
     CDS             48740..49381
                     /gene="plsY"
                     /locus_tag="VH663_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEQ8173359.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            complement(49439..50707)
                     /locus_tag="VH663_000125"
     CDS             complement(49439..50707)
                     /locus_tag="VH663_000125"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEQ8173360.1"
                     /translation="MILIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVEVVAFAQRLGAKVTAEVSPQHFSTTEDLLLTAGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(50839..51492)
                     /locus_tag="VH663_000126"
     CDS             complement(50839..51492)
                     /locus_tag="VH663_000126"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEQ8173361.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPSRENVFK
                     ALQVTPLEKTKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESPHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGVDWLK"
     gene            complement(51625..52275)
                     /locus_tag="VH663_000127"
     CDS             complement(51625..52275)
                     /locus_tag="VH663_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ8173362.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFFTGLFLGLGLALLGR
                     SRQPLIHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWIIAFLLERYQEFLENKLALV"
     gene            complement(52304..53167)
                     /locus_tag="VH663_000128"
     CDS             complement(52304..53167)
                     /locus_tag="VH663_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ8173363.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWETIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DVQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPVIDTKDVN"
     gene            complement(53285..54739)
                     /locus_tag="VH663_000129"
     CDS             complement(53285..54739)
                     /locus_tag="VH663_000129"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEQ8173364.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGKPFAGVPIFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVK
                     RLEDLGFIILGRSNTPEFGFKNISDSSLHEPVNLPKDNTRNAGGSSGGAAALVSSGIS
                     ALAPASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKDSIYQSLQRPLTIAFYQRLNDGSPVSLDTAKALRQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            complement(54936..55565)
                     /locus_tag="VH663_000130"
     CDS             complement(54936..55565)
                     /locus_tag="VH663_000130"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEQ8173365.1"
                     /translation="MTLASQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLNAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            complement(55623..56315)
                     /gene="pyrF"
                     /locus_tag="VH663_000131"
     CDS             complement(55623..56315)
                     /gene="pyrF"
                     /locus_tag="VH663_000131"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEQ8173366.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRYIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            complement(56592..57128)
                     /locus_tag="VH663_000132"
     CDS             complement(56592..57128)
                     /locus_tag="VH663_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173367.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     SAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDVLYEHIQRVNLEE
                     EAFIKYYQSPKVPTGEFE"
     gene            57350..58255
                     /locus_tag="VH663_000133"
     CDS             57350..58255
                     /locus_tag="VH663_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ8173368.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHNKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            complement(58335..59123)
                     /locus_tag="VH663_000134"
     CDS             complement(58335..59123)
                     /locus_tag="VH663_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEQ8173369.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRTIEDQYHCSLWLNQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWHFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRREPFYFDDMNAIIKKELSLWRQSIV"
     gene            complement(59116..59829)
                     /gene="deoD"
                     /locus_tag="VH663_000135"
     CDS             complement(59116..59829)
                     /gene="deoD"
                     /locus_tag="VH663_000135"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ8173370.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVGLETLIAND"
     gene            complement(60492..61301)
                     /locus_tag="VH663_000136"
     CDS             complement(60492..61301)
                     /locus_tag="VH663_000136"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ8173371.1"
                     /translation="MSLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
                     VYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQHIKEDFKGLVKAILSEL"
     gene            complement(61285..61725)
                     /gene="arsC"
                     /locus_tag="VH663_000137"
     CDS             complement(61285..61725)
                     /gene="arsC"
                     /locus_tag="VH663_000137"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEQ8173372.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLTDPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYMKEDGESVNPIEVKGEKDVIDD"
     gene            complement(61744..62955)
                     /locus_tag="VH663_000138"
     CDS             complement(61744..62955)
                     /locus_tag="VH663_000138"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEQ8173373.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     AAGLSVPNMAKIGLGNISRPIPLKTVPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIIANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDTA
                     MIGESFLGHLK"
     gene            complement(63032..63715)
                     /gene="rpiA"
                     /locus_tag="VH663_000139"
     CDS             complement(63032..63715)
                     /gene="rpiA"
                     /locus_tag="VH663_000139"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEQ8173374.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEGLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEAPTAG"
     gene            64093..66192
                     /locus_tag="VH663_000140"
     CDS             64093..66192
                     /locus_tag="VH663_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ8173375.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATNSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKESQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQSQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGSNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNHAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILKEGQLVIKQKDQ"
     gene            complement(66250..66993)
                     /locus_tag="VH663_000141"
     CDS             complement(66250..66993)
                     /locus_tag="VH663_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173376.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKSPSSV"
     gene            complement(67141..67740)
                     /locus_tag="VH663_000142"
     CDS             complement(67141..67740)
                     /locus_tag="VH663_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEQ8173377.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPQDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDDKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTTRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDKSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            complement(67750..68979)
                     /gene="clpX"
                     /locus_tag="VH663_000143"
     CDS             complement(67750..68979)
                     /gene="clpX"
                     /locus_tag="VH663_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEQ8173378.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIKILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            complement(69299..69796)
                     /locus_tag="VH663_000144"
     CDS             complement(69299..69796)
                     /locus_tag="VH663_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEQ8173379.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAIL
                     MGRMTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNLYDFTVT
                     ALKHK"
     gene            complement(69876..70715)
                     /locus_tag="VH663_000145"
     CDS             complement(69876..70715)
                     /locus_tag="VH663_000145"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEQ8173380.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
     gene            70924..72099
                     /locus_tag="VH663_000146"
     CDS             70924..72099
                     /locus_tag="VH663_000146"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEQ8173381.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRAKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTADPRILALNEDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAVCFHIPFPKLALKGLNNIMNNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHQRIYHTNDKN"
     gene            72086..73363
                     /locus_tag="VH663_000147"
     CDS             72086..73363
                     /locus_tag="VH663_000147"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEQ8173382.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDHDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQAGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(73593..74582)
                     /locus_tag="VH663_000148"
     CDS             complement(73593..74582)
                     /locus_tag="VH663_000148"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEQ8173383.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQQVN"
     gene            complement(74569..75582)
                     /locus_tag="VH663_000149"
     CDS             complement(74569..75582)
                     /locus_tag="VH663_000149"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEQ8173384.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILIPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFSHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHPAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQDARDTLISKWQEADIALLYQERWGEN
                     DQP"
     gene            complement(75575..76519)
                     /gene="mvaD"
                     /locus_tag="VH663_000150"
     CDS             complement(75575..76519)
                     /gene="mvaD"
                     /locus_tag="VH663_000150"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEQ8173385.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSVSFLPDTATSDQFYINGVLQNDEEHTKISTIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFDTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDEWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            complement(76501..77379)
                     /gene="mvk"
                     /locus_tag="VH663_000151"
     CDS             complement(76501..77379)
                     /gene="mvk"
                     /locus_tag="VH663_000151"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEQ8173386.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLIIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            complement(77673..78905)
                     /locus_tag="VH663_000152"
     CDS             complement(77673..78905)
                     /locus_tag="VH663_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ8173387.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPDTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSMMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
     gene            complement(78914..79582)
                     /locus_tag="VH663_000153"
     CDS             complement(78914..79582)
                     /locus_tag="VH663_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8173388.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYC
                     LLEK"
     gene            complement(79585..80235)
                     /locus_tag="VH663_000154"
     CDS             complement(79585..80235)
                     /locus_tag="VH663_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ8173389.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETQFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
BASE COUNT        24228 a        16957 c        14210 g        24906 t
ORIGIN      
        1 tctcattttt atattggagg aatttatcag tgaatactta tttttgcaca caccataaac
       61 aattactact ttattcaaac ctattcctta gctttgctat gatgggccaa ggaactgcca
      121 tttatgccga tacactgact tcaaattcag aacctaataa tacttacttt caaacgcaaa
      181 cgcccactac tacagatagc gaaaaaaaag tagtacagtc acaacaaaaa gactactata
      241 ctgaattgtt agaccaatgg aacagtatta tcgcaggcaa cgatgcttat gataaaacca
      301 atcctgacat ggtcactttt cataataaag ctgaaaagga tgctcaaaac attattaaaa
      361 gctatcaagg gcctgaccac gaaaatagaa cttacctttg ggaacatgca aaggattatt
      421 ccgcttctgc taatatcacg aaaacttacc gcaatattga aaaaatagca aaacagatca
      481 ctaatcctga atcatgctat tatcaagata gtaaagctat tgctattgta aaagacggta
      541 tggccttcat gtatgaacac gcttataatc tagatcgtga aaatcatcaa acaactggaa
      601 aagaaaacaa agaaaattgg tgggtttatg aaattggaac tcctcgtgct attaataata
      661 ccttatcctt gatgtatcct tattttactc aagaagaaat tcttaaatac acaactccaa
      721 tcgaaaaatt tgtgcctgac cctactcgtt ttagggttcg cgctgccaat ttttcacctt
      781 ttgaagccaa tagcggaaat ttaattgata tgggacgtgt taaactcatt tccggtattc
      841 ttcgtaaaga tgatctcgaa attagtgata caatcaaagc aattgagaaa gttttcacgc
      901 tagttgacga aggaaatggt ttttaccaag acggttcttt aattgatcac gtagttacta
      961 acgctcaaag tccactttat aaaaaaggca ttgcttacac tggagcttac ggtaatgtgc
     1021 ttatagatgg cttatcgcaa ttaattccta ttattcaaaa aacaaagtct cctatagaag
     1081 cggataaaat ggctactatc tatcattgga ttaaccattc ttttttccct atcatcgttc
     1141 gtggagaaat gatggatatg actcgagggc gttctatcag tcgttttaat gctcaatctc
     1201 atgttgctgg cattgaagca cttcgtgcta ttttacgtat tgctgacatg tctgaagagc
     1261 ctcaccgttt ggcacttaaa acacgtataa aaacactcgt cacacaaggg aatgcttttt
     1321 acaatgtcta tgataatttg aaaacctatc acgatatcaa acttatgaaa gaactactaa
     1381 gtgatacttt tgttccagtc caaaaacttg atagttacgt agctagtttc aatagtatgg
     1441 ataaattggc actatataat aataaacacg attttgcttt tggcctatca atgttttcga
     1501 atcgaactca aaattatgaa gctatgaata atgaaaatct tcatggctgg tttacttctg
     1561 atggaatgtt ttacctatac aataacgatt taggacacta cagtgaaaac tattgggcaa
     1621 cggtaaatcc ctaccgctta cctggaacca cagaaactga gcaaaaacca ctagagggaa
     1681 ctcctgagaa tattaaaacg aactatcaac aagttggcat gactagtctc tctgatgacg
     1741 cttttgttgc aagtaaaaaa cttaataaca caagtgctct agctgctatg accttcacta
     1801 attggaataa aagcctcact ctcaataaag ggtggtttat cttaggaaac aaaataatct
     1861 ttgttggtag caatatcaaa aaccaatcat ctcacaaggc gtatacaact attgaacaac
     1921 gaaaagaaaa tcaaaagcac ccttactgtt cttatgttaa caatcaaccc gttgacttga
     1981 ataatcagct agttgatttt acaaacacta aaagtatttt ccttgaaagt gatgatcccg
     2041 ctcaaaatat tggttactac ttcttcaagc caacaacact tagcataagt aaggcacttc
     2101 aaacagggaa atggcaaaac ataaaagctg atgacaaatc accagaagcc atcaaagaag
     2161 tttcaaatac ctttatcact atcatgcaaa accatactca agatggcgat cgttatgctt
     2221 atatgatgct tccaaatatg actcgtcaag aatttgaaac ctatattagc aagcttgata
     2281 tcgacttgtt agaaaacaat gacaaactgg ccgctgtcta cgatcatgat agtcaacaaa
     2341 tgcacgtcat tcactatgaa aaaaaagcaa cgacgttttc aaatcatagt ctttctcatc
     2401 aaggctttta tagttttcct catcctgtca agcaaaatca gcaataaaag cttgctcatc
     2461 aaggaattgc agctaaaaat aatactctta actctcataa aattccccac aaaagacaac
     2521 gacgcttacc gcatactggg tatcagtcct cctcattaga attcttaggt ggagctttag
     2581 ttgcttcttt taaccatatc acaaaaccct ttcgtaagaa agacttaagg atttagctgt
     2641 tattggaaag taagggttaa caatgaaacg ttattaaaga caaaaaaact ggaaactgca
     2701 ctgagcccca aaagttggct actctatttg aagtaaagga tttagttctg tgttgtagag
     2761 gactaagtcc ttttcgtttt attttgatac ggttattggt gcaataatcg atgataataa
     2821 taggtagaac gagtcaattt agccgtcctt aaaagaaaat ctaatcagaa ctcgtcttta
     2881 tccattcttt ataatggttt tttgtcttct tcttttcaga gatgcttctt tcaaacggag
     2941 ttctctcggt ttttttagcg ctaaatgtct agcttatctt catagcttaa tgtcatacaa
     3001 aaacacccca aacgttaggg ttttatatct aacgtttggg gtgcagttca tagttgtgct
     3061 agtctttttt gtgatctgtt atgtgatgat atttttcatt agttaataag acaaagacgt
     3121 tttacttacg ttttggtggg ttatggatag cttcgccaag cacatctgca aaggcttcgt
     3181 acatcacttc agagaaagtt gggtgtccat gaattgataa taacaattca tcaacggtta
     3241 attcagattc cataatagta gctgcttcgt taatcatctc cgctgcagct ggaccaataa
     3301 tatgaacacc taagatttcg tggtatttgg catcagcaat aactttgaca aaaccatggg
     3361 cttcatttga agcaatcgcg cgtccgttac cggtaaagct attttttccg ataagcacat
     3421 cgccatattg ctcacgcgct tgttcttcag ttaaaccaac cattgccact tcagggtgtg
     3481 tgtaaacagc cgctggtgtg tatttaaggt tagctttacg agttgtgttg ccatgcatag
     3541 cattttctgc agccacttca cccatacggt aagcagcatg agcaagcatt ttcgttccgt
     3601 taacgtcacc tggtgcatag atacctggaa ttgatgtctc ttggtagtca ttaactttga
     3661 tacggttacg atccatttca aggttaaggt tttccagacc gttcatttgt ggtacacgac
     3721 cgatagaaag aagagctttt tcagcgacaa cttcttcacc attgttaagt ttcaaggtca
     3781 attggttatt tgcttcaaca atttcagaga caccaacaga tgttttgatc ttcatgcctt
     3841 tcttagaaag gattttttga agttcaagag agacttcctt atccatagct gggataatgc
     3901 ggtcagccat ttcaataacg gtaacatcaa caccgtaaga tgcccaaaca agtccaagct
     3961 cgataccaac aacaccaccg cccataactg ctagtgattt tggcatttca cgaaggtcaa
     4021 ggatatcgtc agacgttaag acaagtttag agtcaattcc tgggatattg atacgtgata
     4081 ctttagaacc tgtagcaagg ataacgttgc gacccttaat ggtttgtgac ccaatggtta
     4141 cagtcttatc agggttgact tgaccaagcc cattaaagat agtgacttta ttagctttta
     4201 gaagaccttg aacaccacct gttaaggttt taacaacggt gtttttaaag tcaactgttt
     4261 tgtccatgtc aatggtatag ttagttgagg caaggttaat cccacgtcct gctgcaatct
     4321 taataccatc aaggatttca gcatttttaa ggtaagtttt ggttgggata caccctacgt
     4381 tcaagcaagt tccaccaaat tcagatttct caacgatggc aattttacca ccaagttgag
     4441 caccacgaat agctgcataa taacctgcag ggccaccacc aacaacgatg atgtcatact
     4501 catcatcagc aagtgcagct tttggtgcag ctgaagcagc ttgagctggt gcttttggca
     4561 cttcaagtcc agcagcttct aaatcttctg ttgtacgagc aacattcaca tctgaagcag
     4621 ctgggctaga aacctcaaca gattcacctt cagcaccgat atatccgata acttctgtta
     4681 caggtactgt ttcacctgct tgacgtgtaa ttttaagaag aacacctgag tcttctgcct
     4741 caagttccat attggtttta tctgacatga tttcaagaag aatatcgcct tcattgacag
     4801 tatcaccttc ttgttttttc cactcgatga tttcaccttc ttgcatgtca acaccgagtt
     4861 ttggcataat aatttcaaca gccataattt aagataccaa gagcaaggag aaagcgattg
     4921 aatttttgcg gtaagtcaga aatctttgat ttcgttgacg cacccacgtc aggacgacta
     4981 ggactttccg aaacaaaaat tgaggaaagt tcagacgtct acgacgctaa gattagccaa
     5041 ctcttaccat ttttatccga gcttttcgct cggattcaat tgcttatcac tcatttgcat
     5101 gagagctcct cgttctacat cctttctata tttttgagaa aagggcacca agtctaaact
     5161 tagcaaaatc ccttttatta ttgaatgctt ttcttgttta aaactgattg atcagattaa
     5221 caattcaaat ggattttcca tcaatttctt gagatcaacc atgaattttg caccgttcat
     5281 accatcaact aaacggtggt cgatggttag gcacatcgcc ataattggac gggcaacaat
     5341 ctcaccatcc acaaccgttg gagttgggat agttgctcca acaccaagaa ttgctgagtt
     5401 tggttggtta ataattgggt taaatgtctt cgtaccaaac attcccaagt ttgtgataga
     5461 gaaggttgat ccagacattt cagctgcctt caacttacca gtttgcgctt ttttaatcac
     5521 atcttttgaa gcaagcacaa aatctgacaa gctcatcttg tcagccccat ggataacagg
     5581 aacgataaga ccatcatcaa gacctacggc aataccaagg ttcacaaagc ggtgcaattc
     5641 aatatcatta gcatcattga tgagggaagc attcatgtac tcatgctcag gtttcatcaa
     5701 ggttttaacc actgccatac caatcaagtc tgtgaagctg actttaaggc ctgttttagc
     5761 catgattggg tcaattaact tcttacgaag ggcaatcatc tctgtcatgt caatatcgta
     5821 gttaagcgta aaggtaggag cagtaaggta agagtttgtc atacctttag agattgcctt
     5881 acgcatagct gacattggct tgtgttccac accttctgga agatcaacaa ctttttcttc
     5941 tttagctgct ggggcttttg cttcagctgg tttagccgct tcaagaatag ccatgatatc
     6001 ttctttcatg accttaccgt taaagccagt acctgatacg gttgccaaat caataccttt
     6061 gtctgctgca attttacaag caagtggtga agctttcggt tgagcacctt taaagttttc
     6121 aacatcttct ttatggacac gtccttttgg tcctgttcct ggaacttggc caaggtcaat
     6181 gcccatttct gctgctacct tacgtgcagc tggagtagca cgaacttttc cgccatttcc
     6241 ttgcggaata gctgtcgctg ccacttgagg agctggactt gccacatttt cttttggagc
     6301 aactgttggt ccagcatctg ctgaagcagg aactggaatt tctgttgttt tttcacttga
     6361 agcaatagta tctactgatt caccctctgc accaatgtaa ccgataactt cagttacagg
     6421 tacagtatca ccagctggac ggacaatttt caatagcaca cctgagtctt ctgcctcaag
     6481 ttccatattg gttttgtctg acatgatttc aagaaggata tcaccttcat tgacggtatc
     6541 accttcttgt tttttccact cgatgatctc gccttcttgc atgtcaacgc ccagctttgg
     6601 cataataatt tcaaatgcca tttctacatc ctttcacgat ttgataagcg ccatcaacat
     6661 ccccaccttg ctacgaaaac atacgaaagg tgaggtgccg cgacacggga taagacaact
     6721 cagttcttct aaaaactttc aaaatagttt ttagctgaga cgttactgat aaacatagcc
     6781 tttatcggac cacttgcctt atcttctagt tgcctttgtt tgccattttc acaatagcag
     6841 ctttgatttt ctcaacgtct ggtaagatag cttgttcaag tacacgtgca taaggtactg
     6901 gaacatcttc acttgcaaga cgaacgattg gatgatcaag gtaatcaaag gcttcacttt
     6961 cggtaatcat ggttgcaatt tccccaatga atccaccggt cttgtaggca tcgttgacaa
     7021 gcatcaattt acctgttttc ttaacagatt caataatcaa ctctttatca agtgggatga
     7081 gggtacgtgg gtcaacaact tcaacgttaa taccatccgc tgctacttct tcagccgctt
     7141 gaaggacacg ctccaacata cgaccatatg acacaattgt caaatcagtt ccttcacgtt
     7201 tgatgtcacc tttgccaagt gggatataga agtcaggatc ttggttaact tcttcttttt
     7261 taccataaag cgcttttggt tccatgaaaa gaacaatgtt attatcacgg atagctgatt
     7321 ttaataagcc tttagcatcg ttggcattac caggagcaac aactttgatt cctggaatgt
     7381 gagtcaacca tgcttcaaga gattgtgagt gttgcgcagc ggaaccaata ccagaacctg
     7441 atgccacacg gaaggttaca ggggtaataa ggcctccacc gaacatgtaa ttatttttag
     7501 caccattatt cacgatagcg tccatcatga tggtaaggaa atccatgaag gtaacgtcga
     7561 caattggacg tagtcctgta atcgctgcac caatagccgc accagagata gcagcttctg
     7621 aaattggggt atctttaaca cgtttaggac caaattcttc aatcattcca acagaagtcc
     7681 cgaagtctcc tccgtaaacc cctacgtctt ctcccataag gaaaatgttt tcgtccttgc
     7741 gcatttcctc agtcatagca aggttgactg cttcacgcaa agccattaat tttgtttctg
     7801 acatcatttt tacttctcca tttattattc aaatacgatt gcttcttact gacaaggtga
     7861 tcttagtcaa cccaaacgtc ttcgaaggct actgaaagtt ctggatctgg actgttttgt
     7921 gcaaattcat aagcatcgtc aacttctttt ttgacttgtg cttgaatagc atccaattcg
     7981 tcatcagtgg caatgccttc gcttgtcaga taagtacgat atttgatcat tggatctttt
     8041 tctttccact catccacttc ttctttagta cggtatttac cagcatctgc agtcgagtga
     8101 ccaaaccaac ggtatgattc tacctcaaca atagctggac catttccacc acgaacatgt
     8161 tcaacggctt tgcccattgt ttcatagaca gccatcacgt cattgccatc ttcacagtag
     8221 aaaccaggaa ttccgtaagc ttctgcacgt gtgtataaat gtggtgtatt tgttgcatta
     8281 ttgatgctca ttgaaattcc ataacggttg ttgatgatga agaaaataac aggaagtttc
     8341 catgttgcag ccatattaac agactcgtgg aatgatcctt cgttagtagc gccatcacct
     8401 gagaaagcaa cagctatatt gttagttcct ttgtattgtt gagtgagggc agcaccaact
     8461 gcaagagcgt aaccaccacc aacgatacca ttggtaccat agtttccttt ttcaaaatca
     8521 gccaagtgca ttgagccacc acgcccttta gaaacaccag ttgctttacc agcaagctca
     8581 gccatcattt tattaaggtc catgtcttta gcgattgatt gtccatgtcc acggtggttt
     8641 gaaaaaataa tgtcatcgta agacaagtga gccaccgctc caacattggc tgcttcttca
     8701 ccaactgaga agtgagtcat cccttgtaca aatccacgac ggacgagttt attaatacgt
     8761 gaatcaaatt cacgaatacg ttccatttta aggaacatat ctaaatgttg ttctttagaa
     8821 actgttacca tttcggcctc catattatca tattactttt ttatgataaa actttttttc
     8881 acaatttgca aataattaaa tcgctttcta ccttacctta tttggttttt tagaaatgca
     8941 atagtttgtg aaattacaaa caaaactgta ctaaaaaatt taaaaatact atataacagt
     9001 ttcacgtttt accgaccttg acataaaaaa cacatcattt taaaacctct ttcacacctt
     9061 caaacaagta gcaaagcgat aaaaataagc ttagcaaaac tgctaaagct tatttttatg
     9121 attctatttg ttcagcgatg gtttcccatt cttccataag agcttcttgt tcttgatcca
     9181 attggtccca ttccttttgc caagcgatta actgcgccgt atcattggag gcatgcatgt
     9241 cttcttgaat agcaccaata cgctcttcaa tggtttcaag tcttgcttca atctcctcat
     9301 atcggcgggt caagcgacga cgctcttttt gattagcttt ttgtagctga taatcagtgg
     9361 cagaggcttc ttttgtttcc tcaactgtct ctcctgctgc taggcgggct aattcttcta
     9421 gctctgcctt tttctcaagg taataatcat agtcacctaa gtaaagggtt gagccatttt
     9481 cggtaatttc caaaacctta gttgccaaac gattaatgaa atagcggtcg tggctgacaa
     9541 aaagcaaggt accgtcaaag tcaatgaggg cattctctag cacttcttta ctgtcaatgt
     9601 ctaggtggtt agtcggctcg tccagaacaa gaaaattatt gttttccata gataacttcg
     9661 caagaagcag acgggctttt tctccacctg acagcatagc caccgatttt ttgacatcat
     9721 ctccagaaaa gagaaaagcc cctaatcggt ttctgatatc gacttctggc gtagttgaaa
     9781 aatcctgcca taattcttcc aaaactgtgt tgctagacgt caaatgtgat tgggtttggt
     9841 catagtaacc tatttcaaca ttggcaccgt atttcacttc tcctttgatg aaaggaatct
     9901 gtccaataac agatttaatc agagtggatt taccaatacc atttggacca acaatagcaa
     9961 tcgcatctaa tttgtgaacg tctaggttaa taggttcaga taagacatgt tggttataac
    10021 caatagcagc tttttcaacc gtcaacacca cattgccaga cggcttatct gcctgaaaag
    10081 tcatgtgagc tgattttcta gcactagtcg gtttgtctaa gcgttctatt ttttccaatt
    10141 gcttacgtct cgcctgagca cgttttgtag ttgaagccct gacaatattt ttttgaacaa
    10201 aatcttctaa cttggcaatt tctttttgct gtttgtcaaa ctgcttttct tcggctacta
    10261 atttttcagc ttttaaggtc ataaaacgag aataattgcc aacgtagcga tccaatccat
    10321 gaggagttaa atccaaggtc actgttgcca ccttatccaa aaaataacgg tcgtgactca
    10381 caatgattag agctccttga taattggcca agtagttctc taaccaggca atggtttcaa
    10441 tatctaagtg gtttgttggc tcatcaagga ccaatagttc tggcttttct aaaagcattt
    10501 tagccaaggc cagacgagta ttttgacccc ctgacagctc agcgatagtc atctgccaca
    10561 tgctctcatc aaacttaaac ccatttaaaa tggctttgat gtcagattca tacgtaaagc
    10621 cgccttgctg acgaaaatgc tctgtaagca gatcataatc tgtcatcagt cgggttaaat
    10681 cttgacctga aacggttgcc atatccattt ccatctgacg caggcgcttt tcatcttggc
    10741 gtaatgcttc gaaaaccttg agcatttcct catatattgt ttgatctgat tcaaaacggc
    10801 tattttgagc cagataagac aaggttaggt cttttttagt attaacttct ccgctcgttg
    10861 gggtttcttc cccaaccaat agcttgagta aggttgactt accagctcca tttggcccta
    10921 ctaatgcaat tctgtctctt tcatcaactt gcagtgatat attttgaaaa agaacatctc
    10981 ctgagaaaga gcgttctaac ttgtttcctt gtaaaataat catactttca ttctaacaaa
    11041 aaaatgccct tttgaggaca ttttcagcct agtttccaaa agaaatcgaa ctctttttaa
    11101 gtcgttgatt accttcttta gcgctaacaa ggaaagtgga atgactatta ccactcccat
    11161 gacgcgtctt tgcagatggc ttttgtgtta agatatcaaa aatttgcacc acttgctcag
    11221 taactatcga gatcttattg gctaaaacgg aatcactgtg attagaataa tacatcagcc
    11281 ctataataat aatcgttact aataagatag tttgtaaaaa ccctttgata agatgccaaa
    11341 acagtttcac tattagctta ggaagccaca aaatgatcgc cagtaattgt ttcattcgtc
    11401 acctcatgtt gtactaggaa atggttaaga taacctttct tctttttggt agtttatcgg
    11461 taaccactct aacagaactt ctaattgttg gttccaatag taccactcat gtttaccatg
    11521 acctttatga tagtcaatgt caagaccttg agctcgaaaa tcagcaatag ctttttcatt
    11581 agcggcaaaa aggaaatcct cataaccaca ccaagcataa aatttggttt ttccatcact
    11641 ttctgccacc atatttttaa ggtaatgctt atcaaggtca gggtcatcaa actggccaaa
    11701 aacgccctgc caataggcta attctcccag gtttccctca agcaaggtct taggagaaaa
    11761 atccaaagcg cctgaaaacg atgccgcata agaaaaccga ttgcttttga gtgcccactt
    11821 aaaggcacca tagccaccca tagagagccc agcaacaaag gttttttctc gtttctgggt
    11881 catattagga aagaaagctg ctaagacctg cggtaactct tgtgacaacg cccgataata
    11941 gttaagtcca taagccgtgt ctgtatacca acctaggtct gtcgaaggca taacgactat
    12001 caaattggta tgccgcaaca agcgttcaat agctgttcgt ttttgccaag agttttcatt
    12061 gcctcccata ccatgtaaca aatataaaac aggaatatcc ttatcccctt gatctttttt
    12121 agggatttca gactggtcag gataaatcac atttacctta cgctccattc ctaaaactac
    12181 cgaatggtat tcaattgcaa tatttgccat tcattacact cctttatacc aaaataatcc
    12241 tatcacgtaa atctactgta tttgtgggtg aacagtttcc tggtctacca gttgttaaaa
    12301 tcaaagaagg taggcagttt ttgcctaacc ttcctatttt cactaacgaa cttccattac
    12361 aaccggcaga atcgctgggc gccttttggt ttgttcaaat aagaatttag acaaatcatc
    12421 tctgacattc ccttttaatt cgccccagtc aaaggtatct ttttttaagt aattgccaac
    12481 agttgtatta actaattctg cactttctct taaaatatca tgacttttct tgacatacac
    12541 aaacccacgc gtattaactt tagccttaga aataatgcgt ttttcttttt tagaaacagt
    12601 aatagcaaca atgaaaatac catcttctga caagacttta cggtctcgta aaacgatatt
    12661 acccacatca ccgatagcat taccgtcaat catgacatca ctggcaggta cacctccttc
    12721 atggagaaaa ccttcgtcat taagcaccat gatatcgcca cgcttcagaa tatgaatatt
    12781 ctctggaaaa ataccaactt cttcagctag ctttgcatgg gcagctaaat cacgatattc
    12841 tccttgtact ggaaaaagat attggggttt taacaaattc atcaatagtt gcaaatcacg
    12901 cccattagca tggcctgaaa cgcgtaagtt ttgagtgatt aatttaacag aaccaccggc
    12961 cttgtaaatc aagttttcaa cacgcgccac catagcttct ttagccgtac ttggtgttgt
    13021 tacaatataa acaaggtctc cttctttgat ttgaacataa cggtgacgtc ccgctgccat
    13081 tttttgtaag ctattaattg gctcacccat acgaccagct tcaaggatga ttaattcatg
    13141 gtcttcgaat ttagacatat ccttaggttt aattaaaaga cgctcatccg taatcattaa
    13201 tttttctaga cgtaaagctg ttctgacaat gttttctgca tctgttccgg ttaaaacaac
    13261 acgacgccca tgggcagtgg cggaatcaaa tacttgttga atccgaacaa gatttgaggc
    13321 tactgcagct acaatgacac ggccatcagc atcagagata acactgtcca tttcttcacc
    13381 aacttcagat tcgctagcaa tctgatcgtt gcttgtcgca ttgactgaat ctgataaaag
    13441 agctagaacc ccttctttcc caatctcagc taaacgtagc aaatccgtct gataaccttc
    13501 tcttgctgct tgatcaaatt tgaagtcacc ggtataaaca atgtttcctt tatctgttcc
    13561 aataacaata cccatacttt ctgggattga atgcgttgtt ctaaagaacg aaactaggcc
    13621 atctttaaat tcaatttcag tatcactgtc taccacatga aaattattaa atttcttagt
    13681 gctgttatta cttttcacaa atagcttggc taattcaata gtcaattcag atccaaatac
    13741 aggagctgat acttcagcta acaaataagg aagagctcca atggcgtcag catggccatg
    13801 actcaagaaa atcccttgta ccttatcttt gttttcaatt acataatcta aattggggat
    13861 gactaaatct accccaagtt gctcattttc ggggtacttg agccctgcgt ctaaaataaa
    13921 catagaatcg ttaatttcta ccaaatagaa gttttttccg tattcacgga cccctcccaa
    13981 ggcaatcatt ttaatatcag tcatgatact cctttaaata ttttattatt acaaacggtc
    14041 cttacaacgt aagtcgaatt actgttatgt agctatgcta ctatcttcta tagtatatca
    14101 tatttgaggc gttttttctc taaatagcct tgatactttc agattatttt cagtacagaa
    14161 atcaaaaaat cactgtctta ttattagtca atgatttaga tcgttgacta atcaaatagt
    14221 tggtaatagt cagtaatcgt cagttgttct ttttccattt gatttaaatc tttaataatg
    14281 tttccatctt tcattacaat cagacgattc ccataagtta atgcatcttc catatggtgg
    14341 gtaatcatca aagctgttaa gccatctttt gtcacaaact catctgtcaa ttgcattaaa
    14401 gattgactcg tcttagggtc aagggcagcc gtatgttcat ctaataataa gagagctggc
    14461 tttttcaacg ttgccattaa taaacttaaa gcctgtcttt gtccaccaga caaaagacct
    14521 gctggtgttt ctaaatgctt ttctaggcca tttcctgttc gtttaaccaa atcctcaaaa
    14581 ctagctaagt gctctgttat ttttcgagaa gccaaggagc gtttttctcc tctttgacgt
    14641 gcgattaaga gattttcagc cactgtcatc cgaggggctg ttcccatttt gggatcttga
    14701 aagactcgcg ataggtaaag agctcttttt tctgcaggcc aatgcgtcac atcctgacca
    14761 agaatcctaa tttgtccccg tgtcaaactt agcgttcctg caattacatt aaaaagggtt
    14821 gattttccag ctccatttcc tcctaaaata gttaaaaaat catgttcata gatagttagt
    14881 gtcacattgt ccaatattgt tttagcatct tcaaaaccat tatctacgtc aactgttgca
    14941 ttgatcaatt caataatttt tgtcatcgtg ttaacctcac tcccttaaag aagcgctctt
    15001 ttaccactgg tatcatgagg cacagtgcta aaacaagagc actaattaat tttaaataac
    15061 ttgtattaaa acctagcgta ataactactg aaattaaaaa ttgataaaga atcgagccta
    15121 ttacaatagc aatcaaacgt tctaaaagag tcagtccagt tgagtatagt acttcaccaa
    15181 caattatgct cgccaaacca ataacaataa ctccaatccc ttttgagaca tctgcatagc
    15241 catcttgttg actcactaga gcacctgata aagctattaa actattagaa accacaagtc
    15301 ccataacttc catccaatcc gtgttaatac caaaactctt agccatatct ttattgtctc
    15361 ccgtagcgat ataagcttga cctagattag tgtaaaggaa atagatcaaa acgctaataa
    15421 caatcacaac agttatcaga cctgttaaaa ggctatttaa atcaattgaa aaaggtaaac
    15481 aatcctgaat acgtttatga tcatgtaaac ccaagtttgc tcttcccatt accattagca
    15541 taatggagtt acatgacgtc atcaccaaaa ttccagctaa aagtgttggc atttttcctt
    15601 tcgtatataa cagccctgtt agaaaacctg ctaaagcacc tgataacata ccaagtaagg
    15661 ttgatagaaa tggattccac cctaagctaa tagcggtgac cgcaacagct cctcctaaag
    15721 gaaaagaacc ttctgtagtc atgtcaggga agtttaaaat cctaaaggtc aggtagattc
    15781 ctaatcctaa aactccccat ataaggcctt gtgaaactga tgaaataatc atatttttcc
    15841 tcatatttat attagcgggt aactctcgcc agtataaacc ctttctttac attaattttt
    15901 agatttgttt tgtacttagt tcagcaccgt ggcttagtct ttttttcaat cactttgacg
    15961 aaactaagac agaaaacaaa aaagtcgaga atcatgttta aacttactca aaaggcaaga
    16021 ttttttacgt gtctaaaata gagcatttta aaactaaaaa tgataaaaat aacccttaca
    16081 tcctcaagac gattgtctga cttggcatcc aaattcatct cagtgagggt gttttttatg
    16141 gttttttctt gaccttttat atcacatgtc attgtattaa ttctcaataa aagaagctga
    16201 aggaaaatct tcccttttaa tagcaatacc taattctgct gctacattct tattaattag
    16261 cggttgacca atatccacaa ctttgacagg aacatctaca acacgctttc ctttgattaa
    16321 ttttaacacc tgattagctg tttgaactcc caaatcgtac tgattttgag aaatagctgc
    16381 caaaccacct ttttcaacca ttcgatcaac gctagtgtag acaggaatct ttgcagcctt
    16441 actggttgtc atgactgatg aaaatgcact tgctatagta ttgtcttggg ggataaatac
    16501 cgcgtctact ttacctaaca taactgacat cgtggcagga acttcgttag tagaaggaac
    16561 cgcgtatgaa ataacttgat agcccttttt gcgagctaac cgcctaaact ctttaacttg
    16621 agataaggaa ttatcctcat tacttgcata taaaattcca actgttttga catgtggtgt
    16681 cagctttttc attaaatcaa ttgtctgttt aacaggcact ttattagata gaccagttac
    16741 attagcttca ggttgatcca attgcatcac taatctagat cctactggat cagatactgc
    16801 tgacatgaca actgggatat ccttgctggc agctgctaag ccttgcgccg ccggggttgc
    16861 gataccaatc acgatatcac ttcctgattg cactaattgc ctactcattg tttggatttt
    16921 actctgatcc ccctcagcat tcataacctt aatcactaca ttttgttttt gaggcatctg
    16981 ctttttgagt tgatcttcaa tacctcgctt aatatcatca agagcctcat gagtaacgaa
    17041 ttgtaagata ccaattgtaa tgttttgtcg gttagccaaa tcacggtttt cctttgatac
    17101 tccttttgat aatagagagc ctatcactat tacagttaaa acaaggaggg tagctattaa
    17161 actcttgttt ttcatctgaa ttcctcacat ttcatgtcaa tctctttaaa acgttgatga
    17221 tgagaatact tctcaaaaga aaaagcactt agtaatgatt actaggcgct cttgtgaaat
    17281 ccaatgtcag cgcatagata tctttttaga agtactctaa aagtcgtatc tatgccaatt
    17341 cataaacaca acttatctat gccagctttg ccaatttact aagtgttgtg tttttaccat
    17401 cattgacttc ctttctcgtt tttttaacat tataacctaa atcaaaaaaa ttgcaagctc
    17461 tttcgtccat atttttagtc aagtatttgt ggtataatga agagattaga attcagaaag
    17521 gagaaaccat gtcttttgac ggctttttct tacaccattt aaccaacgaa ttaaaagaga
    17581 acttgcttta cggtcgtatc caaaaggtca atcaaccctt tgaacgggaa cttgtcttaa
    17641 ccattcgtaa ccatcgcaag aattataaat tgctattgtc tgcccatcct gtttttggaa
    17701 gggtacaaat cacccaagca gactttcaaa atcctcaggt acctaatacc tttaccatga
    17761 ttatgcgtaa atacctccag ggtgcagtca ttgagcagtt agaacagatt gataatgacc
    17821 gcattatcga aatcaaggta tctaataaaa atgaaatcgg agatgctatt caggcaaccc
    17881 taatcattga aatcatgggg aagcacagca atatcatctt ggtggaccgt gcagaaaata
    17941 aaatcatcga atccattaaa catgttggct tttcacaaaa ctcttaccgc accattttac
    18001 ctggctctac ctacattgag ccccctaaaa cagctgctgt taatcctttt acaattacag
    18061 atgttcctct atttgaaatc ctccaaacac aagaactaac agttaaaagc ttgcaacagc
    18121 attttcaagg tttggggcga gacaccgcaa aagagttggc cgagcttctg accacagaca
    18181 aattaaaacg gttccgtgag ttttttgcta gacctactca ggcaaatctg accacagctt
    18241 cttttgcgcc tgttcttttc tcagatagcc atgcgacgtt tgagacttta tcagagatgc
    18301 ttgatcactt ttatcaagat aaggctgagc gtgaccgtat caatcagcaa gccagtgatt
    18361 tgattcaccg tgtgcaaact gaactggaca aaaatcgcaa caagctaagt aagcaagaag
    18421 ctgagctgct agccactgaa aatgctgaat tattccgtca aaaaggagaa ttattgacca
    18481 cctacctcag tctggtccca aacaaccaag actctgtgat tttagacaat tattatacag
    18541 gggaaaaaat tgagattgcc ttagacaagg ctctgacacc aaatcaaaat gctcaacgtt
    18601 attttaaaaa ataccaaaag ctaaaagaag ctgtcaaaca cttatctgga ttgattgccg
    18661 ataccaagca aagtattact tactttgaga gcgtagacta caatttatct caagcaagta
    18721 tcgacgatat cgaagatatt cgcgaagaat tatatcaggc tggctttttg aaaagtcgcc
    18781 aaagagacaa acgccataaa cgtaaaaagc ctgagcagta cctggcttca gatggaaaaa
    18841 ccattctcat ggtgggacgc aataacttgc aaaatgaaga actcaccttt aaaatggcta
    18901 aaaaaggaga actctggttc catgctaagg atatccctgg aagtcatgtc atcatcaaag
    18961 acaatcttga cccaagtgac gaggttaaaa ctgatgctgc cgaactagct gcttattatt
    19021 cgaaagctag gttatcaaat ctcgttcagg tcgatatgat tgaagctaaa aaattacaca
    19081 aacctagtgg cgccaagcca ggatttgtga cttatactgg tcaaaaaacc ttacgggtta
    19141 ctcctgacca agccaaaatt ctctctatga aattatcctg ataagcaact aaaaaacagc
    19201 tctcctagga aagttatttc ccctaggaga actgtttttt tattctgatt gttggtgctt
    19261 gatatggtca agaaagtctt gcatattcac atcatcaggc acttgtctga ggtaagcggt
    19321 agccatttgg atagccttgt gaaactggcc aaactccctt aataaatagg cataatcctg
    19381 taaaaactct ggattttctg ccaaatcagc cgaaatctca ttataaagag ccaaagcaac
    19441 ttcttcttgt tccaaggcgt ggtaagcctt tgcaatggtc catttggtta agacattatc
    19501 aatcgtctca cgattaagag caataacctc ctcaaaacgc tcagcatcaa aataaagggt
    19561 cactaaacgc atgagaatct cttcgtcatc aacagctact tcctttgctt gaagcaggta
    19621 attttcagca ttctgcctat cgtgtaattc ataagaaagc tgcgatgcta gtaataataa
    19681 ttgactgtca aatgcatttt tacgtaggcc ctgttgaacc aagcgcaaag cttctgacgt
    19741 cttatgctct tcatgaagac tcaaagcata accatattca taacctggat aatctggatt
    19801 gatggtttcc aactgtttaa agtaaagatt ggctttttga taattttctt ggtcatacat
    19861 gagggtcgcc aattcaaaaa cagtttcatc ttcgtattca atggccacgg ccttttccaa
    19921 gaattcgatg gctgcctcaa atttccctaa gctagcatag gctcgtccaa tgcgttgata
    19981 agtggaaata cctgtcaatt caaggatttg acgattgtct aactgtgcat agtaatcaat
    20041 agcttcttta aaatactgca aggacatgtc gatttctgcc aaaccaaaaa tgaccaatgg
    20101 ctctgagctg atggccactg cttgaagcaa tttttcacgc gccacctctg tcagtccttc
    20161 caggtcatag aggtcagcca tgactaagag agctgacaaa taattagggc tatcttttga
    20221 aaccttatcc aaatacaaga aagcttcttc aatggcgtcg tcttctgctg caatctgagc
    20281 taaatttata ttgagttctg ggtaatcatc agctaactgc aaataaatac gcttggcatg
    20341 tggcaaaaaa cctatacttt ctaagtattc tcctaaggcg attaaactat ctgcatcatc
    20401 ttcttttaaa gctttttgga aatatttttc agcgtgcgct aggtcttgct gatccaagga
    20461 agcaatcatt ttttcactat ttaacatggt ccttaacctc ctcgatcacc aatacttctt
    20521 cgtcatacaa accgctaact tttttgtacc agtcaaataa ttctttaatc acgactttga
    20581 cagaagcgta gattgggatg acaaggaaaa caccccagac accaaacata gagcctgcag
    20641 tcaataacaa gaacataatg gtaataggat gaatgctcag cttatttccc aaaacaagcg
    20701 gtgctacaaa acgcccttcg atggtttgct caatcataaa gataacaagc accttaacca
    20761 acataaatgg gccttgtacc atagccataa tcacaacagg tatcatggca aggaaacttc
    20821 ctaagtaagg tatcatgtta agaaagccag caataatccc aaaagtgatg gcatatttca
    20881 gtcctacgag gctaaacatg atagaaaaca taaagccgac aacaattgca accgtaacct
    20941 gcccttggac atagcctgat aattgcttgt taatatctcc caagactcga accataggca
    21001 cccgtaattt gagaggtaag acattcacaa ggccattttt catgtgacta ctgtctcgta
    21061 aaaaataaaa gagaataaag ggcgaaataa taatagccac ggtaatccga gcaatggcac
    21121 cagcaaaatt accagcccag tcaatagcat tttttgaaaa actttgcgcg taatccaaag
    21181 ccttttgact tgtatgggta agcatgtctt ggagttgggg cttgtagctg actaaccatt
    21241 cattttcaag aagtttattg gcttcttcat tgaccttgcc cacgtatttg ggcagatcct
    21301 caataaaact ggtaagttgg gtctgtacca tgggaacaaa gcctgaaatc gcccagacaa
    21361 aaagtaaggt aatcaaacca aaaacaataa aaatagaggt ggtacgattc gggcccaagc
    21421 gattaataag gtcaactaag ggtttggtca gataatacag aatggtcgaa ataaccaagg
    21481 gcaacatgat gaccgcaaaa aaagaaatca ctggcataaa taaaaaagag attttagtaa
    21541 aaacaaaaat cgttaaaaag gctaaaagtg ttattactaa agccatggta gcttgattat
    21601 ttaaaaacca tttgtaaaat aagctatctg ttctagactt gtcaggtatt aagggcatta
    21661 agatagggtt cctttctttg ggaaaatgat aagttttact ggtaacctgg gtaaatcaat
    21721 aaaaggtcac agatttatcc atgagttggt tgttttggtc ataggtaaat ttggcagaga
    21781 aaaaaggacg gtcttctaaa atccatttaa aaatgtcata atcaccttcc caagttggct
    21841 tttccaacac ttgatcgtaa ggtacccatt ccaaggttcc ttctcgtgac tcttcatctg
    21901 aaatgaggtc tccttcaaat cctgttactt taaaaacata ggtataccag tcatgaccag
    21961 gggtaaattc tggaaaagta atgattcctt taaaggccat ctctgtaaca gtcaaatggg
    22021 tttcctctaa aatctcacga cgggcacact catctggggt ctcaccagcc tcgagcttac
    22081 ccccaactga aatccacttg cctttgtgga catcattttc ttttttattg cggtgtaata
    22141 atagcaaaga atcgccatta tcaatgtaac aaatggttgc taattgagtc atctatctct
    22201 ttcctttcca tctttgtttc tattatacca atctctagaa aatatgctat cattttatga
    22261 gtcaaagtgt gaatacttag tcacgctcaa aaccttctaa agatgcctta aaaaagtgta
    22321 gataagagcc tacaatgaaa aacatcaatt taaactatca aaagaacctt gtcatatcaa
    22381 cgataattga tatgacaagg ttctttaatg ttttatttaa tcacttcttg agttttagca
    22441 tacttagctt ctacagcatc tttttctgct ttccaccatg tctcattttc tgtgtaccac
    22501 ttaatagttt cttccaaacc ttctgaaaag tttgtaaatt gtggttccca gccgagttct
    22561 tcacgcaatt ttgtagaatc aatagcgtaa cgaagatcgt gaccagcacg gtcagttacg
    22621 tgatcataag catcttttgg ttgacccatt ttctcaagga taagctcaag aacttccttg
    22681 ttatttttct cgccgtcggc accaataagg tatgtttcac cgatacgacc cttagtcaaa
    22741 atagcccaga cacctgttga atggtcattt gtgtggatcc agtcacggac gtttttacct
    22801 tcaccataaa gttttggttt gattcctgcc aagatatttg taatttgacg tggaatgaat
    22861 ttttcgatgt gttggtatgg tccatagtta tttgaacagt tcgaaatggt cgctttcaca
    22921 ccgaaggaac gtacccatgc cttaacgata aggtcagaag ctgccttagt tgatgagtaa
    22981 ggtgatgatg gattgtattt tgtttcagca gtgaattttt cacctggtcc ttcaccctgt
    23041 cctggaaggt cttcacgaag tggaagatct ccataaactt catcagttga cacgtggtgg
    23101 aaacggatat cgtatttacg agctgcttca agcaaagtgt aagttccgat aaagtttgta
    23161 tggataaatg gacttggatc ttccaatgag ttatcgttgt ggctctcagc cgcatagtga
    23221 acaatagcat ccgttttggc agccaatttg tctaccaatt cagcgtcagc gatatcacca
    23281 acaactaact caacacgatc accaagaata gcttcaatgt tagcacggtt acctgcatat
    23341 gtcaatttat caaggacagt tacatgaaca tctgggtggt tattgtagac atagtgtaca
    23401 aagttagaac cgatgaaacc agctccacca gtcacgataa tatttttata cataagatat
    23461 aaagggacct ttcgtttccg aaaaaatcta ttaaaagtaa taactctaac aaagacgttt
    23521 taggcctaag aaaacatcgc tttctaagat ttgagtccca tgttcagctc aaagagagaa
    23581 actgaacttt cctttcatca tttttagttg ttacttgtta cctgaagtag agaagaataa
    23641 ctcccctcat atccacctca aatgattaat cccaatcatc tgagtatgtt gtttccctat
    23701 aggtcttttg gtttcagtgg tttgacatca ctaagaagag gatggttttt atctgcttca
    23761 gaaacttctg cagctgctag attttcccaa gtaatcccaa gacttgggtc agcatagttt
    23821 accaaagcat acttaggttt caagtcaaga gcccagtagt cattgacaag ataactgtaa
    23881 gagacagtct ctgaaagaac ttgaaagcca ttagcaaccc ctctaggaac aaacattcct
    23941 tttgaagcat caatcactgt ctggtagacg tttccaaaag tctctccctc acgaagatca
    24001 acccaagctc ctaaaacctt gccatcatca gcaactgaga tgtatttatc ccaaggttca
    24061 gcatgaagtc cacgcagcac atgctgacgt gaaaaagaaa cgttgttttg tagttttcct
    24121 tcctcaaaaa aacgttctgg aaagccaatt ggcaacattt tttctttttg gaaattttct
    24181 ttaaaccagc cacgattatc cccacgaaca ggaatatcaa actctagtaa gcctgggatt
    24241 tctttgattt ctcggcaagc caatggtttg tcaaaaaaag tttctgtcat taagcttctc
    24301 caatcaaacg aagcaagtat tgcccgtatt cgtttttctt taaagattgc gccaatttat
    24361 ggacatcttc tttactgatg taacccatgc gataggcaat ttcttccaga tttgccactt
    24421 gagcgttttg caaacgttga acagtttcaa tatattgagc cgcttcaagt agactttcat
    24481 gcgttccagt gtctagccat gcaaaaccac gccccatcaa ttcaacggag aggtcaccac
    24541 gatccaagta agccttgttg acgtctgtaa tttccaactc accacgcgcg cttggtttaa
    24601 tgtttttagc aatctctacc acgtcattgt catagaaata aagtccagtc actgcaaagt
    24661 gggacttagg cacttctggt ttttcttcga tagagatagc attcatgttc tcatcaaatt
    24721 ccaccactcc aaagcgctca gggtctttca cttgataacc aaaaacggtg gctccttttt
    24781 ctttggcagc tgccttttga agcattttag tgaggccatt accgtggtaa atattatctc
    24841 ctaaaataag agcaacacgg tcatcaccga taaactcctc tccaataatg aaagcttgtg
    24901 ctaaaccatc tgggcttggt tgttccttat aggataagct aatgccaaac tctgacccat
    24961 ctcctagtaa ttcttcgaaa cgaggaagat cttggggggt tgaaatgatt aaaatatctt
    25021 taatccctgc caacatcaag gttgacaatg ggtaataaat cattggtttg tcataaatgg
    25081 gcatcaactg tttagatgcg gcacgggtca gtggatacaa gcgtgtccct gaacctcctg
    25141 caagaataat acctttcata gtagtgctcc tattcttcta tataccctat cattctatca
    25201 ttttttgaaa gcagtgtcac gctttaccag cagaccacaa aaggtaaggc tcaggggaat
    25261 agtcgctagg cgttaaaagt ccttcgtgtc ctagcaataa ttgagctaat tcatagccaa
    25321 tcaaaggtcc aactgttaat cccgatgacc ctaaaccact agctgtataa aggttcttca
    25381 atcctggaac ctgaccataa aaaggagaat aatcactggt ataagctctt attcctacac
    25441 gacacgtttt ttgggtagct tcttttaata acggcaaata agtaagagct tgcatttcaa
    25501 gtttttctaa aacctgccaa tcaggctcca aatcataacc tttatcattt tcgtgactcg
    25561 caccaacaga aatcttacct tggttaaaag gaataagatc tatttcacct tctggcataa
    25621 caactggata agtgtcgctg atgatgtgat gaacgtcata atccaagagt tgtccctttt
    25681 gtggtcgcac atcaacctga tagcctaaag gcctcaataa gtcaggtagc catgctcctg
    25741 ctgccagaat gacctgatca aaataatgac cagcaatctc atacccactt ccttgctgtt
    25801 tcagagtgac tttttggcga ataactggat aaccactggc cttgagtaac gtctcacata
    25861 aagcggctcc ctccactcta gcagctcctg aagcgtacag acagttatca aagcctatca
    25921 atcctttaaa atcattcccc agttctttac gatttttaat ggctaactca ccaataatcg
    25981 gtgattctac ctttcttgct aacgctaatt cataaagatc tcttagtttt tcttcctgct
    26041 tttttaaaac atagatgcca ttttgctgat aaaaatctgt tgcaaaacca tcttctttga
    26101 ggtcattgat taattgctga taaaagtccg ctcctaaacg agccatgcga taccaaactt
    26161 tattgcgacg ctttgaaaac caaggactaa taataccagc agcagcctta gtggcttgtc
    26221 cttgaccatg gtcaaaaatg gtaacttctt tttgaccact ttgttgtaaa taataagcgg
    26281 cggttgatcc tacaatcccc gctccaacaa ttgctatttt cattcctggt atccttttat
    26341 cttattttaa gaccttaaca gcctataaat ggctaaatgg attggtcgag gctttgcttg
    26401 aaataatgct gacatcccaa ccgttctctt ctttccaccc ttggagtttc tcctttaatt
    26461 tctccgtaaa aagcacctca atatgatgcc ccgggtcaac cgcaaatagt ccttctgtca
    26521 gcatctcctg tgctgtatgg taatagatat cccctgtaat ataaacgtct gcgccttttt
    26581 gcacagcatc ctgataaaat tcaccaccac ttccgccaca gatagcaatc tttgaaatca
    26641 aagggttttc cttatcgtaa cggataagtc gcacggtatc taaatcaaac acccttttga
    26701 ccttacttgc taactcttct aacgcttgtt ctttaactgt tccaatacga ccaatgccaa
    26761 agccttcttt agtttcagac aagtaagttg cttccttgat ttctagcagg tcacaaaacc
    26821 aatcatttag tccgcctggt acaatgtcaa tattggtatg gctcacatag atagaaatgt
    26881 catgcttgac caaatctaga aggatatcac gctgagggga tgaaaccaga tctttaagcg
    26941 gtttaaagat gggagcatgc ttagttataa tgagatcaac ttcatttttg atggcttctg
    27001 ctactgttga ctctcggata tctaaagtaa tcataacctt acgaatgtct ttatccaatg
    27061 aacccatttg aagaccttta acgtctcctt ccatagataa atcaagtggg caaaaggctt
    27121 cataggcatc aattagggtt ttagctttca tgaacaatca cctcttttat ttgttgtatt
    27181 tttgtcaaga gaagctgacg ctcttgagtt ttttcttctg ggatgcagga caaggcataa
    27241 gctaacttat ccatttctcg ttgccacttc tctttaaaaa cgactgattt ttcctgagaa
    27301 aggtatggac caaaacgcaa ttctgtcgcg gataaggcct tttcaccatg ctcagcaacg
    27361 atgatttcat aatatttgtc attttcagcc atgatggttt ctgcaacaat tttaaaagcg
    27421 ttaaccgata accaagcccg taaatcatcc tcacgattgt tgggctgtaa aaccagacgt
    27481 tcaatgccct gcaatttttc cttacctgcc tctaaaatat cagcaatcaa acggccaccc
    27541 atgccacaaa tggtaatggc tgtcacatca tcagcttctt caaaggctgc aaggccattg
    27601 gctaagcgca cctgaatatg ctcggcaagg ccagactgtg tgacattttt aagagcagat
    27661 tcgtagggac ccctaaccac ttcgccagca atggctgctg aaatctgatt agtttctacc
    27721 aaaaatatgg gaagataggc atgatcactg ccaacatcta ataatttgac accttttgga
    27781 acataggccg caacttgggc taaacggttt gataattgac tatccatctt gatttctttc
    27841 taaaacgtaa tggttctatt atagcaaaat tacctccata aaaaaagaaa accaaaggct
    27901 aggctttcga ttttccataa gtattttgat agtacttgca gtaagactga acgggacaaa
    27961 tctcacattt tggttttttg gctaaacaat ggtagcgtcc aaaaaaaatc aatcgatggt
    28021 gagtaataat ccaatctttc tttggaattt tagccatcaa atctgcttca atctgcttga
    28081 catcggcatc tggtgatgaa atattgagcc tctttgagac tctagccaca tgagtatcaa
    28141 ccgcaatagc tggaacacca taaacctctg ccaataccac attggctgtt tttcggccaa
    28201 cacccggtaa actttcaagc tctttgtggg tttttggcac ttgaccctta aaatcatcac
    28261 gaatagcctg agcagtttta atgatatttt tggccttatt tttatagagt ccaatcgttc
    28321 tcagagcatt ttcaacatca gaaagttcag caaaagctaa gtcttctatt tctggataag
    28381 actgccatag tccaggagtt accttattga cggccttatc tgtcgtttga gctgataaaa
    28441 tcaccgcaat caacaattga aaaggtgttt cccagtctaa ctctccctta gcctcaggaa
    28501 acatttgacc aatgatggtc aggacttttg ctaatcttgc ttttccaatt ctcatgaatc
    28561 actccataaa ttcatcgcag atagaaaatc ctctgatatc gtcacttgag aaagatcttc
    28621 gccttctcta acacgtctac gttcttctac ttgtcgaaga ttaacaatac cttcttttcg
    28681 ccaattacgt aagatagcct ggatatattt ccaatttgtt ttgccgttga agacagcttc
    28741 ttttaaagct tctcgtatca aatcaggatc tgttttatca tcacgaagcg ttttttcaag
    28801 atcttctaac tcaaaaggac tcaagaatcg tcctaattca cgttcaaatt catcaactaa
    28861 tcgtttgaaa tggtttggtg tttcttgttt gtcaatttca gttgccgttt gactgacaaa
    28921 caattgatct aacttggcta acacaggact cgcatcaaaa ataatttcaa tttctccagt
    28981 aagttcaatt gtccgcatat ctaaaagccc ttgactagtc aaagatgaaa tgattttatt
    29041 aatatcagca actgattttc caagcgcatg agcaatttga ctgggtgcta agtcatctcg
    29101 ctttgtcgta ttttgaagat ggaaaaactg ccagaccaaa aaatcatctg agctcttaaa
    29161 aagatctttg taatgaaaaa gcaaggcact tgggataacc aaattacctg acttataatg
    29221 ttctaaaaaa ctcataatcc ctcaatcaaa aggctaaaat cgaatctata gcctttacta
    29281 ctttaaatcc cctaatcaga tgctgattaa tgtgcttacc caggaaactg cattaagact
    29341 ttatagtcat agttcctaat agcatggcga ccatttaaac cttctaattc aataaggaag
    29401 gcacaaccag ctacaatacc accaagtttt tcaatcattt caatagttgc ttttaccgta
    29461 ccgccagtcg ctagtaaatc atcgacaatc aaaacacgtt gtccaggctt aatagcatct
    29521 gcatgcatag ttaaggtatc aagaccatat tctttttcgt aatcagcaga gacaacatct
    29581 cttggcagtt ttcctggttt acgaacggga gcaaaaccaa ttcctaactc aactgcaaca
    29641 ggacaaccaa tgataaaacc acgcgcttca ggtccaacaa ccatatcaat atctttatca
    29701 caagcgtact gtgctatttc acgaatagca tagctatatg ctttaccgtc agccattaaa
    29761 ggtgagatat ctcgaaacgt aataccagct ttagggtaat ctttgatcga tgcaatgtaa
    29821 tttgttaaat ccatagttaa ttctttctag tggcaaattg ccaaatataa aatactcttc
    29881 attatatcac gaaacatatt tttcgtaaaa taagacttct gacattattg tttatctgat
    29941 acttcattcg taactacctc aataactact gtcaactgct ttacttattg atcgtttgat
    30001 ttcaccaaat aatcatacat ctctttcggg ctagccaaag ccatgatttc ttgaaatttc
    30061 accaattctt ttaagtcttg gtaaatgtgg ctctctgaaa tctctcgttt ttgcgcttgg
    30121 gggttaaccg tcatcagccc atcgtcaatc gtcacaaaag acaactcttc aaaaatttga
    30181 atcaacttaa tcagtaaaag tttttctatg tttaaatagt gactcaactc tgtcaattta
    30241 tgccgtaaat caaattcagg aaactgatag atggttttgt agagcttggc aaactgctct
    30301 ctgctgccaa atcctgttaa ataataagga tgtttcatct gatttttaaa atagatggcg
    30361 tcaaaatcct tgtgaacaaa ctggttgcgc caggctttga aatcttcagg gatgtcatta
    30421 atcagaatga ctctggcatt tgggtcttct tcaatagtcg gaataccttc aggaactttg
    30481 gctgtttttg tccttaagtc aagaagttgc actccgtcaa cacgagcatc tactaacatg
    30541 aattggagac tcgtgttccc attccaatgg ttgacagata aggtaactgc taattctaaa
    30601 ccagtagctt gtcggaactc ttgcagttga cttccttgac caaaagccag tacgtcaaag
    30661 gaagctttgc cctgactgac tttaaattta agatggctct ggtcttgccc tatggtacgc
    30721 gcttgactaa cccttatgtc cttgacataa aagaccggct tttgatgatc catcccataa
    30781 ggggctaatt tatctaaact ctttaaaata tcaaggctaa gatcgtccaa agacagtctt
    30841 tcatcaatag ttaacgtatt tttggcagtc tgatctaagc cacgttctat cacaaactga
    30901 caaagaaagt ctgagagagc ttccaagttg tcaacgggca gggtcattcc tgctgcacca
    30961 gcatgtcccc caaaggctgt aaataactca cgcttgccat ttaaggcttc aaaaatatta
    31021 atagcttcta aacttctggc cgagcctttt gcaaagccat tatcaatagt caaaacgacg
    31081 accgtttgtc caatagtttc catgatacga ccagcgacaa tccccagaac accaggatgc
    31141 cagccagctt gtgccaagac ttgaacaggt ttttgaggat caaccatagc catcgcttga
    31201 tcaaaaatgt cttggactaa ggccttacgc tcttcatttt ttttcttaat catcaaggca
    31261 atcgcttgag cttcttggtc atcaaagccc gtcagtagct caatggctgg attagggtca
    31321 tctaagcgcc ctaaagcatt taattgcgga gcaatctgaa agccaatggc gtcttcatta
    31381 aaatgttcca aatcaatagg agacaaggac atcaactcct gcaagcccac tctttcggtg
    31441 tgtttgagca tggccagacc atttttgact aaaacacggt tttcacctgt caagcttacc
    31501 atatcggcaa tggttccaat agctaccaag tctagacagt ctgttggtaa actttctagc
    31561 aaagctgttg ccaatttaaa ggcaacgcca cagccagcta agtgtttaaa gggataatca
    31621 gcatcaggat gctcaggatg aatgatggca aaggcttctg gcaattcttc aggcaggcta
    31681 tgatggtcag ttacaatgac atcaacttcc tgttcttgag catatctaat cgcttcatgt
    31741 ccggccacgc cattatcaac cgtgataatt agggacacag cttcctgctc aataaaatat
    31801 ttatagacgc tctggttggg gccatagcca tctgtaaagc ggttgggaag ataaaccaaa
    31861 ggctctgctc ccatcatgtc tagggtttct ttgacaatag aagccgacgt catgccatca
    31921 gcatcatagt cgccgtaaat gagaattcgt tcgccctctt caatagcttg acgaattctt
    31981 ggaacagctt ttgccatgtc atgtagcaag taaggatcat gcagttgaga caagtccgct
    32041 gttaaaaact catctaaggc ttcttcagtc cttatccctc gatcataaat caactgagcg
    32101 gcggtctggg ttagaccttt tgtctttgct agcttaaaga agccatcatc tggcttcttg
    32161 tcttttattt tccagctata tttggattta atcatttata attaaatacc tttcttgcaa
    32221 gataatcaga taatttaggg aaaagggtgt aaaattgatg ggtcaccgct aaactaaagg
    32281 gcaaattcaa ttctcgttta tttttcccga taatagaaac caaacgctta gccacttgat
    32341 ttggttggag agtaaattta ccaacgcttt ccaaataatg tccagacggg tcagcttggt
    32401 taaaaaactt ggtggcaatg ggacctggat tcacggtggt cacgtaaacc cctttatccg
    32461 ctaattctaa gcgaagggca ttggaaaatc cgataagggc aaacttggtg gctgaataaa
    32521 tgctcgattt ggctgacgca atcaagcctg ccatggacac aatattaata agatgacctt
    32581 gcccctgctc tgccattttc tgaccaatca agcaagcaaa gtgaatgctc gctagggtgt
    32641 taacctgaaa catatcagct atttctttgg cagaaaactc ttcaaagcct ttgaaagctc
    32701 cataaccagc attattaatc aagacatcaa tacggccata gcgctggtaa atctgggcga
    32761 ctattttctc aatggctact ggattggtaa tatccaactc aaggcattct ttgttgtcaa
    32821 tatgctgata acagtgttct aggcgttctt tgttacgtcc taataaaatc aagctgtctt
    32881 ccttgggtaa ctgcttaaca attgcctgag ccagtcctcc agaagctccc gtgataacaa
    32941 tgattctttg tgccattaaa taatcacttc ctctaaatct tgtaccattt taacattttc
    33001 aaatatagtg gcggcatctt tttccatctg gcgacaatca cgccctaaaa agcgagctga
    33061 tacatgattg agcaagaggc gttttgcgcc tgcttcatga gcgatttgtg ctgcctgcat
    33121 gttggtagaa tgcccgtgat ttctcgcaat ccgctcgtca cccttaccat aggtggattc
    33181 atgaaccaaa acatctgcat ctttagcgag tttaacactg gcagacgttt tgcgcgtatc
    33241 gccgataatg gtgatgattt tgcctttttt aggggcagaa atataatctt tggcacaaat
    33301 caggcggccg tcttccaact caacgtcttg accattttta attttgccaa agagtggtcc
    33361 aaaaggaaca cccgctgctt ttaaggcttc tgcgtctagg gttccttcta agtctttttg
    33421 aactactcga taacccatgc aaaaaatggt atgcgccaga cgctctgcat agacctcaaa
    33481 cttatctgtt tccattattt tacctaatga tttgtcatca aattcatgaa aatgaatctg
    33541 ataaggtaca cgcgctccag aaacttttag actagttaac acgtaagttt taatgccaat
    33601 tggtccataa atatcaagat ctgtctgttc ttcactagct tgaaaagacc gacttgataa
    33661 aaatcctggt aatccaaaaa tgtggtctcc atgcaaatga gtaatgaaaa tctttcttat
    33721 ttttcgtggc ttaatcgtcg tttctaaaat ttgtctttgc gtcccttcac cgcaatcaaa
    33781 catccagact tcattgattt cgtctagtaa tttcaaggca agacttgaca cattgcgctg
    33841 cttggctggt tgaccagctc cagttcctaa aaattgtaat tccattcaat ctattctttc
    33901 tattgggtga tgtaaatcat aaattctcgt tgggcaaaat cataatactt ttgaccagaa
    33961 aatgtttggc taagagccca taactcctct acattaaacc ccttaagcat ggtgataccg
    34021 tttgccagac gataaatctt cgacaagagt gtgtcatcag gcgtaatgtc ttcacattct
    34081 ccttgctcaa actgcttagc ctgcatttta agctggcctt tgtcttcata aaccatatat
    34141 tgtgggaaaa tttgtgttaa ttcaaagaag agttcgggtt tcttaggctc tgctttttca
    34201 gaaaatacca atgcaacttc ttggtcattt tggtaagcaa aaccataagc ttttccagac
    34261 aaattgaggc gtacaaaggg cttttcttcc tcaaaagatg gttggtcagt aaaaagcccc
    34321 aaagccttag aaagattaaa ataataatca gtcgcagctt gcggtgactg tttctggtag
    34381 atttcagcaa agtaggcatt aataacactt ggtggtggtg tgataaaagc tagtttttgc
    34441 tggtctgtca aactggctaa cagatggttt aggtagttgg tatccagact agtaaatcca
    34501 ccataggttt ttgctaaatc aatataatta ttcataaaaa tcgtcaagtc tccattgctg
    34561 ttttggggaa atgtagcccg aaatatcctc tatttcttgg tcaaacgtgt agtggtccaa
    34621 aagagcaact cggttcaaat catacagctt gtacgccttg tcttgatgca ctttgattct
    34681 aaaaggaaca aactggtctc gaatttggtc aatgattaaa cgtctcaaga gaattttgct
    34741 atcctttgaa cgggctgaaa tcctgatatt tggaaaagca gtagctgtga attgttctgc
    34801 tatatccacc ttgttataaa tagctaaacg tggaatatta agcatgtcta gctcttttag
    34861 aaggtttaaa accacttttt cttgctcact atgattaggg tcgctagcgt caatcacatg
    34921 taacagcaaa tccacatatt tgctttcttc tagggttgat ttaaaagcag ccactaattc
    34981 cgttggcaag tcttgaataa agcctacggt gtctgttaag gttgcttgaa attgattttc
    35041 taaatagagt tgctttgtag ttgcatctaa ggtagcaaat aattcattgg cttcatagtg
    35101 gctgtcatct gtcaacaagt tcataatggt tgacttgcca gcattggtgt agccaatcaa
    35161 gccaatttta aaggtatctg aacccacacg acgatctcta atggtttgac gatttttctc
    35221 tacctgagtt aattggcgtt caatatcagc aatctgatga cgaatcgaac ggcgatttaa
    35281 ctctagttgg ctttcacctg gtccacggct cccaatacca cctgcttgac gacttaacat
    35341 gatgccctgt ccaaccaaac gaggtaacat atatttcaat tgggcaaggt gtacttgtaa
    35401 tttgccctca tggcttctgg cacgcatggc aaaaatatct aagattaact gcatacggtc
    35461 aatgactttt acctctaaaa cagcttctag attagcattt tgcctagcag tcaaccgatt
    35521 gttcacaata actgcatcaa tctcatcagc ttccacaatc gccttaatct catccagttt
    35581 ccctgagccg ataaaggttt tgctgtcgta acgttctcgt ttttggctga aacttgccat
    35641 aactttgacg ccagctgtct tggcaagatt agctaattct gtcatggaca tgtcaaaatg
    35701 ttctgttgtc tgaagctcaa ctcctaaaag aatcactctt tcttgctgac gctttgtctc
    35761 aaccatcacc tttaccaatc ctccaaattc cgtaaattct attataacat atcttcaaca
    35821 gattctgcta attttgcgtc atgacttctc tttctgacca agaaaatccc tgaccctatc
    35881 atgtactacc tgtgggtaat caggagccgt tatggcagtg aagctaacag ccattcgatt
    35941 gcggaaccaa gttagctgtc gcttggcaaa acgtctcgtg ttttgtttta actgatcact
    36001 agcttctgcc aaagtcattt cacctacaaa ataggggaat aattccttat aacctatgcc
    36061 acgactagcc tgaacagtcg ggtaatgctc atataaccat tttgcttctt ccaacaagcc
    36121 gttttcaagc atatgatcca cgcgctgatt gatacggtca tagatgactt ggcggtcatc
    36181 attcaagccg ataataagcg gctcataagc cgtctcggca ttctctaatt catcagcaaa
    36241 ctgagccaat tcaagggctc ggatcgcccg cctgcgattc acctgctcaa tggtaatgtt
    36301 attgacttgc aagcgttcat acaagtcgtg atcgtctagt tgctccaact cattccgata
    36361 ggcttttaca gcctcttggt ctactgttcc tcctaagtgg taaccttcga gtaaactttg
    36421 taagtaaaga ccagttccac ctacaataat tggtaatttc ccacgactaa caatgtctga
    36481 aatagctttt tgagcatctt ggacaaactc gtaggccgaa taagactccg tcacctcacg
    36541 aatatcaatt aagtgatgaa ccgctgcttc ttgctcttct tgagtggcct tggctgttcc
    36601 aatatccaac tgtcgataaa cttgctggct atctcctgaa ataatttctc cattaaaagc
    36661 cttggctaaa ctaatcccaa gtgctgtttt tccaaccgct gtcggaccaa caatcacaac
    36721 tatttttatt tttgtcattt tcttgtcttt ctattagaaa agcgtgtcca aaaacgcctt
    36781 ttacttgaaa attagcttat ttttcgctac catatattat aacataagat taaggaccac
    36841 tgaggtcctt cactagaaat ggagaataat catggttaag aaatatcaat ttgttaaagg
    36901 tcttgctact ggcgtcctag caactgctgc tactgttgct ggtgctgtct ttgctgtaaa
    36961 aaaaacaatc attgaccctg aagaagaaaa agctgctttc attgaagaaa accgcaaaaa
    37021 agctgcgcgt cgtcgtgtag ctcgctaacg tgacaatgga tcaatttaaa agagactagc
    37081 acattaaagt gccggcctct tttttagtaa tctcgataac tagttagtag taactgtctc
    37141 ttgatgagcc aagatgttag ctgctttact tattttgaaa tagaaatctc aggtaagccc
    37201 cataaacata cgtgactaaa gctgtcgcca acaatacctc tcctagcaaa agaacctgcc
    37261 aacctaaacc ttgccaacct aaaaaagata aggtcatttt taaggaagtg gcactaatca
    37321 caaacggaaa ggtaaaagct gaaaaacctg ggttaaaggg acgattgagt aagcgaggaa
    37381 gctgtacaac cacaaaagca tataacagtt gtgagctgat gagaagaaag ataaccattc
    37441 ctacttgtgg tctaggaaag gttgctaaat aagacgctga aagtaaggac agaggggcac
    37501 aaaacgttga aatattgggc attactgctt gacctaaacc aatcccataa gtctttttgg
    37561 ccataaaagg taaaatcaca gcagttaaga gaagacaaac ccaaaaaatc acctgtccta
    37621 ataaaaactg cctactagca ggtgctgtta atgccgccat tgcaattcca acaaaaagca
    37681 cggaccagga aggagaaacg ttatcccatg aaaaagaaaa gacaaaacga tactggtaat
    37741 aagcaatcag gacaagattt ccccaaaaga atagccacca agtgagaaag cctaaccaac
    37801 accctcctgt cactttgaga aagagtgaag acaacaacat ccctgtcata aaaaaagttg
    37861 gaaaaacaga ggcaattaga ggttgtcgta actgcatttt ggtatcgtta aggttcctaa
    37921 ccatgccaac aagcaagata ccatagataa aaagagctgc cagaattccc aaataattaa
    37981 aaatcgaaac ataagtagcc aacaaattgc caaatgacaa cgttcctaat gccaatcctg
    38041 acatgaccaa tggtgggttt ttaaggtgtt tcataagtgt ccttttctgc ctattaacag
    38101 ctatttttag tgacataatc ttgtttattt tatcacattt cgagcatttt tgacaccacc
    38161 cgcgaaagaa aatttaaggc tcttgccatt gtttttttac attctcgcaa aactagcaaa
    38221 ctgcttaaaa agctaaggag taaccactct tctctagaaa aatgaaagag tagagaagac
    38281 ttggtctgat ctttacttct tctttttgat ttagtgagct gcattaagca cttgttgaac
    38341 agctgaatct tccacaattt ttgccataaa ggctgccaga tgcggataat tctcgtaaga
    38401 ttttggtgtt ttttgagacc atagcgccat ggcgtaagca taagcatctg caatggtttg
    38461 ttgatctcga taaacatgcc cactctcccc aataagacca tctaaatgcg tcatagcaat
    38521 acccatccgc ttgtaagaag cttgtttgac tttttcaagc gcttgattgt cttgagaaat
    38581 agtgtagcga tgagggttga acattggcct aaaaacaaag ggaaaatcac ctgttaagta
    38641 gtccatgatt tcgttgaacc gactgtctgc caaaagatta tcttggggaa tgagctgaga
    38701 tttcagggtt tgtttcgcta taaagggcaa gataatacct gcctgtgtga caatttgacc
    38761 atcatcaagc tgtaaagctt aaaccattcc caaaggattg atggtaaggc agtcttcaga
    38821 atccagtttt accttctcta cctcgtaata cagaaactca caattctttt gaaaaggttc
    38881 tagacagagt tggtgttgac gtcttcgctg ttgatggctc tgcatgacaa aatgagcttg
    38941 gtgtccacgt tcacttgtat cttgttgcaa agtctttgca atttggtctc atcatctgta
    39001 tcacaaaaaa caaaccagtt tcctgatttg ttttgagata ttagacacct acaaattctg
    39061 gcaaggattc tccgatagct tctaaacgtt ctgcccattc ttgacgtgat aaaccatttt
    39121 cttgcacata gcgattacgt tcatgtgcac gacattcagg cgagcaccct cgaacatact
    39181 tcgtttcgtt ttcttctgaa gcaaaaattt gtttgttaca aaacggattt gcacaattga
    39241 cataacgttc acaaggtgtt ccatcaaaat aatctttact aatgacagtt ggattaacat
    39301 gattaattgg caccgagata cgatcatcaa aaacatacat cgcaccatcc caaagttcac
    39361 cttgaacttc cggatctttt ccatatgttg caataccacc gtgtaattgt ccaacatctt
    39421 taaagccttc tcgaaccatc caacccgaga atttctcaca gcgaacacca cccgtacagt
    39481 acacgacaac acgtttttcc ataaacttat ctttgttgtc acgtacccat tgtggtaact
    39541 cacggaaatt gcgaatatca ggacgaattg ctccacggaa gtgacctaag tcatactcat
    39601 aatcattacg cgtatctaaa acaacagtat cttcatcaag caaggcttct ttaaattgtt
    39661 taggatttaa atactcacct gttgtttcca aaggattaat atcactatca aagttattgt
    39721 cttctaaccc aaggtgcaca atctctttct tataacgaac aaacatttta cggaaagctt
    39781 gttgattttc ctcgtcaatc ttgaaccaaa gatcagcaaa acgctcatcg ctgtggaccc
    39841 aatccatata cttctgagtt gtttcataat ctccagaaac agttccatta atcccctcat
    39901 ccgcaatcaa aatacgacct ttaagcccaa tagatttaca aaattctaga tgtttcgcag
    39961 cgtactcttg cgcattttca attgaaacat acttataata gagtaaaact ctgatttttt
    40021 ctgacatttt ttctccttca ttgttattca gttacttcct attatatcta ggaaaaacaa
    40081 gtctaggcaa gccatttgtt tgtctttttt ttcacaaaat agatctcaat ttatttttga
    40141 tgagatggca atccattact cctttaagca aatcattaga agctttattt agaaaatgct
    40201 aagataaaac aaggaggcca aaatgactta tgaactttgt ttagaatatg ggacttatcc
    40261 gttgtcacgt gttgatgctt actggggaga agatcaaaat cccccaacat tcattcaaga
    40321 agatcgactg ctttgccata aactagaaac tatgaatcac ctgttccatg atttatttgt
    40381 aactattgaa tcacaattcc attatgttgg ttttaacatg cctgaaaaaa gagcgcagat
    40441 tcggatacta tatcaagaag tagctactat attaaaatgc aaatataagg attatccaat
    40501 taagattgag acgtttcttc tttaaaaatc taatactaaa aaactaactg tgcttagaca
    40561 gatgacacag ctagtttttt aggtaaaact cttcttaaat tttaaaacgt ttcgaaaaat
    40621 cactaaatgc tttaatagca cttgctaaaa atggtttcac catctcatca ataattaacc
    40681 ttgattactt acaatcttat gaatctgagt caaataaaat tcaggctgtg gcattacaga
    40741 gacatttaaa acctatactc atcgttttca aggtcagaaa gagaatgatt ttgaaaaggt
    40801 tcgtcaactc ttctagataa ttttggacaa attttgatca agaaacaaaa agagcctatc
    40861 aaataaggct tttttatatg atttctatcc accttgaggg aatcgaaccc ccatctcaag
    40921 aaccggaatc ttacgtgata tccattacac taaaggtggt cacaactttg tcaataccct
    40981 tatagtatat cataaaatga aataatatga aataactttt ctaaccagaa gacaataaaa
    41041 agttgaggaa aacctcaact ttttagattt attacaattc aatatcaccg aaaagatcag
    41101 ccattgagaa tccagtttgt gtttctggga gttcgtagtc acgtcttgat tcacgttttg
    41161 gacgacgtgg gcgtgattga cgtttttctt ctttgttgtc tccttcagct tgcgctgggc
    41221 gttcttcaag agctttgatt gataatgata cgcgctcatc agctgcattc acttcaagaa
    41281 ctttaactgt tacttcttgt cctacagaaa gtacatcttt tggattttca acacgtttgt
    41341 gtgaaatttg tgaaatatga acaagtccat caatacctgg taatacttca acaaaagcac
    41401 cgaagtcagt caagcgtttt actttacctt caacaacatc accttgagca agtttttgtt
    41461 caacgccgtc ccatggtcca ggtgttgttg ctttaagtga aagtgacaca cgaccagctt
    41521 cttcgtcaat tgaaagaact ttaacttcaa cttcttcacc aactgaaaca actgatttag
    41581 gtgacacgtt acgttcgtga gacaattcag tcacgtgaac aagtccgtca acaccaccaa
    41641 ggtcaatgaa agcaccaaaa cttgttaaac gcgctacagt acctgtaacg actgcacctt
    41701 ctgaaatctt agagaacact tcagcacgag cttctttagc ggcctcttcg ataacttcac
    41761 gacgtgatag gatgaaacgg ttttctgctg catcaacttc tttgatttta gcatcaaact
    41821 cttgtccaac aaatttttca gtgttgcgaa caaaacgagt gtcaatcatt gaagcaggga
    41881 taaatccacg aagtccttca aattcaactg aaagaccacc tttaacagca cgggtacctt
    41941 taacagtaac aacttcgcct tcacgaccaa caagtttgtc ccaggcttta cgagcttcca
    42001 aacgtttttt agatactagg aaagtaactg tatcagtatc tttacctact acttgacgaa
    42061 gaacaagtac ttcaactgtg tcgccagctt taacaaagtc gttaatatca gcatcgcggt
    42121 cgttagtcaa ttcacgaagt gtcaagacac cttcaacacc tgttccttcg ataacaacgt
    42181 ttgcttgacc gttatcaaca gttaaaactt ccgcagtgac aacatcacca gggttcactt
    42241 cgctaacact gtttagcaaa tcttcaaatt cattcattct aaaaaatcct ccaacaaaag
    42301 ctagccaatg ctagcttttg ataacaatat attttctcaa ggtaacccgc aacgacaaca
    42361 actcttatct ttgacggtct cagggagcac ctgatacctc tgactggggt agctggattc
    42421 gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata ccccaaaaat
    42481 attttttaca gaaaactaat tttctaatta gattagtttc cgaaatggaa agagagggat
    42541 tcgaaccccc gaacccgaag gagcggattt acagtccgcc gcgtttagcc tcttcgctat
    42601 ctttccgtaa caacaaaaac tattctatca tagtttttgt aagctggcaa gtgtttttgt
    42661 ctaattttaa tggttcaaat tgtagttttc aatgatattt tcaaccgctt gttcgagcgt
    42721 tttgaagaag caatctactc catcattttt taagacagca aatttctcat caagttctgc
    42781 aatttctcca accacacgtt taccaatttg taattgatag ccatcaatct tagtttcatt
    42841 aaccataact ttgtgatcaa tcagttggat ttcaattttt ttatcttttt tactcatcac
    42901 tttacctcac tttgtctatt ttacacaaaa tcaaaaaagc ttgcaagagc aagctctatt
    42961 ttaatctact tttacaatcc aaccttctgg tgcttcaata tccccaaatt gaataccgac
    43021 caactcatcg tataaacggc gtgtcacagg accaacttct gtttcactgt aaaagacatg
    43081 taagttatcc ttgtactgga taccaccaat tggagagata acagctgctg ttccacaagc
    43141 tcctgcttcc acaaatttat ccaattcatt aattggaaca tcaccctcaa ttactgtcat
    43201 accaagtctt tcttcagcta gttgtaagag tgaatacttc gtaatagatg gcaagataga
    43261 tggactaagt ggcgtaacaa attcattatt tgcagtaatg ccaaaaaagt ttgcagctcc
    43321 gacttcttca attttagtat gggttgctgg atctaagtaa atcacatcag aaaagcctgc
    43381 tgatttagcg gccttacctg gtagcaaact tcctgcatag tttcctccca ccttagcagc
    43441 acctgttccg taaggagcgg cgcggtcaaa cgcttcagaa acaataaaat tagtcggagc
    43501 aagtccacct ttgaaatagt ttcctactgg catggcaaaa atggtgaaaa tatattcttc
    43561 ggctggttta accccaataa tatctccaac tccaattaat aacggtctca ggtaaagcgt
    43621 tgcacctgtt ccgtaaggtg gtacaaagtc ttcattggct ttaaccactt gcttagctgc
    43681 atcaataaat tgctctgttg atacttgtgg catcaacaag cggtctgctg tggcttgtaa
    43741 acgcactgca ttacggtctg gtctaaatag ttgaatagag ccatctttgg tgcgataagc
    43801 ctttaaacct tcaaaagctt gttgcccata atgaagcgct ggagcgcttt ctgagatatg
    43861 taaggtggca tcttctgtta attgaccttt atcccattga ccatttttgt aataagaaat
    43921 gtaacgaaat ggcaacttat gatattcaaa tcctaggttg tcccaatcaa ttgctattgt
    43981 catcatcgta cctcttttct gcttttttct attgtaactt tttttcaata attttcaagt
    44041 ttattctact cttttgcatt aatattctaa atttaccata aaattaaaaa gagttttcac
    44101 tctttttaac gtgttctagc taccagtact tcttgatccg aaatagtgtc tgaaataaag
    44161 gagccattgc tggttctttc cgataacgaa agcgtttcta aagcaatctc gtaggtttga
    44221 ccagtcttac taatgacttc aagcatttgt tgattagttt cttcttcagg tatatcagtg
    44281 aacaaatcaa actgctcagc tgaagtatct gattgaacgc ctccggccag aaaaactcta
    44341 tgaggtttgg tttttaactc tcgtaggacc aatagtccac gattggcacg acttgtctga
    44401 ggaatatcat cgacagccat ccgtttgaga gaacccctct gagttaagac aaagaaagag
    44461 ttacttgtca cagcaaaggc tgaggcaagg ctgtcatcat ttttcaagtt aatgccttta
    44521 acaccagcag atttcaaccc ttgaataggc acttcctgac tggagaatcg caaagcataa
    44581 ccatttttag tcactaatag gaggtcttcc ataataactg gtgacaaggt aaccacgcgg
    44641 tctttgtcat cctttagctt cacatatttg gttgatttag atttataagt tcgccaagga
    44701 cttagttctt tacgatcaaa gcgtttgata aaaccatttt gtgtgacagc cacatacaag
    44761 ccctggtcaa aggaagtcac aatatcagcg tagagaatct cttcttctgt cgcgaagttt
    44821 gaaatggttt gtgataaatg ttcaccgata tccttccaac gtaaatcggt caattcatgg
    44881 attggtcggt aaatgacatt gcctaaagtt gtaaacaaca aaaggtgttc tgttgttttg
    44941 gcctgtttaa cgaagatgag ttcgtcatca tctctcttgc caacttcttc taaactagac
    45001 gcattgaagg atcgtgggct ggttcttttg aggtatccgc cccgtgtcac actaacaaaa
    45061 gtttcttcct ctgcaatcaa gctggcagtg tcaatctcaa tgatttgact ttccgcttgt
    45121 aactctgata aacgaggatt ggcgaatttc tttttcacct ctcttaattc acgtttcatg
    45181 acgttataca tggttgcttc gtcaccgata atagcggaaa gggtcgtgat taagtctctt
    45241 aaatcatttt cttcattttg gagagtaaca atatcggtat tagttaaacg ataaagctgc
    45301 aaggttacaa tcgcttctgc ttgttcctcg gagaaatcat agctgacttt cagattttct
    45361 ttagcatcag ccttattgtc agacgatcta atcaaagcaa taatctcatc taagatggac
    45421 aagacacgaa tcaatccttc aacaatgtgg aggcgttttc cagctttggc tttatcgaat
    45481 ttagaccgct caataataat atctttgcgg tggctgatat agcttgataa gattttttga
    45541 agacctactt ggcgtggcgt aaagtgatca atagcaacca tattgaaatt gtaattaact
    45601 tgaagatctg tgtatttcaa caaataattc aaaatggttt ggctatcagc ctcttttttc
    45661 aactcaatag ctatgcgaag acctgtgcga tcagattcat cgcgtacttc tacgatgcct
    45721 gggactttat tgttaacacg cacatcgtca atctttttga ccaagactgc tttgttaact
    45781 tcataaggaa tctcagtgac aataatttgt tgtttaccac ctttaagttc ctcgatttct
    45841 gtcctagagc gaacaacgac acgtccctta cctgtttcgt aggctttttt gatttcgtca
    45901 gctccttgga taatgcctcc tgtcgggaaa tctggcccag gcaaaaattc catcaatttt
    45961 tctaaactag ctttgggatg atcaatcata tacacaacag catcaatcac ttctgacaaa
    46021 ttgtgcggtg gaatatctgt cgcatagcct gcggaaatac cagaagaccc attgacaagt
    46081 aagttaggaa aagctgctgg taaaactgtc ggttcttttt ctgtgtcatc aaagttccaa
    46141 gcaaagggaa ccgtattttt ttcaatgtct tggagaagat aacccgcaat ttcagacaag
    46201 cgagcttctg tataacgcat agccgcaggc ggatcaccat ccattgaacc attatttccg
    46261 tgcatttcca caagaatttc acggtttttc caattttgac tcatccgtac catggcatca
    46321 taaatagagg aatccccatg cgggtggaaa ttccccatga tattaccaac tgatttggct
    46381 gatttacggt aacctttttc aaaagtattg ccatccttat tcatcgaata aagaatccga
    46441 cgctgaacgg gtttcaatcc atctcgaata tcaggaagtg cccgttcctg aataatatac
    46501 ttggaataac ggccgaagcg ttctcccata atatcctcaa gggacatgtt ttgaatgttt
    46561 gacatattca aatagctttc tttttcatta tttattaatt acctaaaaaa caatcgtctt
    46621 tagcacttgt aattatctgt gagaaacttg tatgactaaa acaccgtatt ttcttctaag
    46681 gtaaacttga cgttatcctc gatccactga cggcgtgggg ctgctttgtc acccatcaac
    46741 acagaaacac gtcgctctgc tcttgccaag tcgttaatag taacacgaat aagggttctg
    46801 gtttcagggt ccatagtggt ttcccataat tggttagcat tcatttcacc cagtccttta
    46861 tagcgttgaa gaatagcccc cttgccaaat tctctgcgca gatcttccaa ttcaccatca
    46921 gtccaagcat aagcaatttt ttcagttttg cctttacctt ttgacatttt gtaaagcggg
    46981 ggaagcgcaa tataaacgtg acctgtttct actaatggtc gcatataacg gtaaaagaag
    47041 gtcaataaga gggtttgaat gtgggcacca tcggtatcgg catcggtcat gataataatc
    47101 ttatcatagt tgatatcctc gagattaaag ttagctccca ctccagctcc aatggtataa
    47161 accatagtgt taatttcttc atttttcaag atgtcagcca ttttagcttt ttcggtattt
    47221 aaaactttgc cacgtaatgg taaaatagcc tgaaatttgc ggtctcgtcc ttgtttggct
    47281 gaacctcccg cagaatctcc ttcgaccaga tacaattcat ttttcttggc atttttggac
    47341 tgggctgggg ttaattttcc cgacaacaag cctttgtctt ttttgttttt cttaccatta
    47401 cgtgagtcat cacgcgcttt tcgagcagct tcgcgagcat ctctagcttt aatggctttg
    47461 cgaactagat gagaagctac ttctccattt tcgagtaaaa agaaggttaa tttttcagaa
    47521 acgatactat cgacaatcgg tctcgctaag ggacttccca atttgtcttt ggtctgtcct
    47581 tcaaattgca agtgctgctc aggaactaag atagataata ctgctgagag tccttctcga
    47641 taatctgagc cctctagatt tttatccttt tcttttaaga gattggtttt acgagcatag
    47701 tcattcatcg ctttggtaat agctgacttt agtcccgttt catggcttcc accatctttg
    47761 gtgcgcacat tgtttacaaa agataagata ttgtcggaga aaccatcgtt atactgcaaa
    47821 gcaacctcga cttgaaagtc ttggtcttga ccttccatgt aaataacagg tgtcaaggtt
    47881 tccttatctt cattgaggta ttcgacgaaa tcttgaaccc cattttcata atgaaactct
    47941 tcaataatcg ggtcatctcc acgaagatct gtcaagctca tcttgacatt tttcaaaaga
    48001 aaagcagatt ctttcaatcg ttctgaaatg gtattgaact taaagtcaat agttgaaaaa
    48061 atcttatcat caggcataaa tgttacaacc gttcctgatt tagacttagg agctgtgcca
    48121 acttttttaa gggttgtaac aggtttcccc ccattttcaa agcgctgacg atacactgac
    48181 ccgtctcttg tgatttcgac ttctaaccag gcagacaaag cattgacaac agaggagcca
    48241 acaccgtgca aaccacctga cgttttgtag ccaccctgac caaatttacc acctgcatga
    48301 agaatcgtaa aaataacttg aacggtggga atccccatgg catgctggcc tgttggcatt
    48361 ccacgtccgc tatctgccac actgaccgag ccatctttat tgataacaac cttaatatca
    48421 tctccaaaac cagacaaggc ctcatcgaca gcattatcaa caatttccca aatcaggtgg
    48481 tgcaatcccg tagcatctgt tgaaccgata tacatccctg gacgcttgcg aacagcatct
    48541 aacccttcta ggacttggat agcatcgtca ttataattat taattgtaat ttcgttctta
    48601 gtcaacaccg acctccagac agtttcatct tttttatctt acaagttttt taccaatttt
    48661 gcaaaataat ttgctaattt ttcccgaatt cttttccaga tctagttctc caatctatga
    48721 atatgttata ataaggatta tgaaattact actttttatt accattgcct atttacttgg
    48781 ttctattcca actggactat ggattggaca gtacttttac cacatcaact tacgagagca
    48841 tggatcagga aatactggaa ccacaaatac ttttcggatt ttaggtgtca aggcaggaac
    48901 agctacctta gctattgata tgtttaaagg gacactttca atattgttac caattatttt
    48961 tggtatgact tcaatttcat ccattgctat cggctttttc gcagttttag ggcatacttt
    49021 tcctattttt gccaacttta aaggtggtaa ggccgtagca acaagtgctg gtgtattgct
    49081 aggctttgct ccgttatatc tctttttttt agcatctatc tttgttttag ttctctattt
    49141 atttagcatg atatctttag ctagtgtggt ttcagctatc gttggtgtgt tatctgtttt
    49201 aacatttcct gccattcatt ttcttttacc aaattatgac tactttttaa cttttattgt
    49261 gatattactt gcgttcatta ttattataag acacaaagat aacattagtc gtatcaaaca
    49321 tcatactgaa aatctaatac cttgggggct aaatttaagc aaacaagtgc ctaaaaaata
    49381 aatttactaa aagcctgact ttctctgaaa gtcaagcttt tttgttttaa ctcatttgtt
    49441 aattagggta aacaacctct ccgtctgcaa tagtgtattt cacgacacct ttgagtttgt
    49501 taccaataaa tggggaatta ctagctttag aagcaaaatt ctcagtgatc agacgttctt
    49561 gcttgtctgc aaaaataacc aaatctgctg gaccattttc tgccaaataa cccgcatcaa
    49621 aaccataaag caaagctgga ttaagcgtca ttttttctaa tagtgacata agtgtaagat
    49681 gacctggttc aaccaaatgc gtcaaaccta atgacaaaga agtttcaagt cctgtcatac
    49741 cagatggggc tttagtcata tcatcaacag ctttttcatc cttgtgatga ggagcatgat
    49801 cagtggcaat gactgtgata acacccgatt tcaagccttc tatgacagct aagcgatctc
    49861 tttgtgtacg aaggggagga ttcatcttgg cgctggtacc tgcggtcaat aagagatctt
    49921 cagttgttga aaaatgttgt ggggacactt cagcagttac cttagcaccc aaccgctgag
    49981 caaaagccac tacttcaacc gattctgctt tagataaatg ttggatatga acatgagctt
    50041 gcctgtcata agcaatcatg acatctcttg caatcatact atattctgct acaccagttg
    50101 ccccacaaaa atgaaaatgc tcctcagcaa taccttcatt aaaaccaaga accccattta
    50161 actgaggatc ttcctcatgc aaagaaatga aagtttggtt ggcattagcc aaatcaaagg
    50221 cttcttttaa aactttagaa ctttctaagg gaataccatc atctgaaaag cttactgctc
    50281 ctgcttccaa caaagcttta aaatcagtca cgtcttttcc attaaaggct tgggtgacac
    50341 tggcattggt gtaaatatga atcttttctt tagctgcgct agctaacact tcctgtaaag
    50401 tttcgacatc tgaaatgaca ggattggtat tagccatcat gacaaccgta gtcacaccac
    50461 ctgctgctgc tgccaaggct cccgtgtgaa tatcttcttt gtgcgtttga ccgggttctc
    50521 taaaatgcac atgaatatcg accaaacccg gagcaacaat aagaccacta gcatcaatca
    50581 cttgagcttc ttggcattca atagctgaag ctatctttac aatctgttta ccatcaatca
    50641 agacatctgc cacttgatct ctctgcgatt tcgggtccat aacgcgtcca tttttgatga
    50701 gtatcatgtt ttctccttta tttatctgtt ttaaccataa gaccttacgt tctttaatag
    50761 gaaaaatcta ctgcgctcat ttcacttatt aaaggtatca aaatgtctag cttaagctat
    50821 ttagttgaaa tggctggctt attttagcca atctacgcca gtcatgccct ctttttctaa
    50881 aatagcgttg gttcttgaaa agggtttgct gccaaaaaag ccacgataag atgacaaagg
    50941 gcttggatga gggctctcga tgatgtggtg ctttggatta gtaatgaagg cttttttctt
    51001 ccttgcataa gctccccaca aaatgaaaac tacagggctg tctttctcat tcagcacttt
    51061 aataacagcg tctgtaaatg gctcccatat taagcctgca tgcccattag cctggcctgc
    51121 tggcactgtt aagcaagcat tcaaaagcag aaccccttga ctagcccaag tgcttaaatc
    51181 atgatggtca cgaggaccaa tgtcatctgc taattctttt aaaatattaa taagggatgg
    51241 cggagcagaa atctcttctg gaactgaaaa tgataagccc tgggcctgtt tggggccatg
    51301 ataagggtct tgtcctaaaa tcaacacttt ggttttttcc aaaggagtga cttgtaaggc
    51361 tttaaagaca ttttctcgtg aaggatagac aagaccggaa gcataggctt catctaaaaa
    51421 gtgattgatg cgaccgtagt aatgctccgg caaaaatgat ttgattttct catgccaaat
    51481 tgaatgagcc ataagattcc tttctaatca cgtcagtttc taagttaaat acgatgtcct
    51541 tttctatcta gtttaccaca ttttaaatag aaagaccctg aaaatatgat atttccaggg
    51601 ttaatattcg ttttttacag gatattaaac aagtgctagc ttattttcta agaattcttg
    51661 gtaacgttct aacaaaaaag caataatcca atagataaag gctactaaaa tgtacatgga
    51721 catgtagtcc cactccctac caccgataat tttggcattt tgaaaaatat ctggtacggt
    51781 aatcatggca gctagcgacg aacttttaac catatcaata atgacattgc ctagaggagg
    51841 aactgcaatt cgaaaggctt gaggaagaat aattttctta accataagag catatggcaa
    51901 ccctaaagcc ttagctgctt cccattgtcc cttatcaata gcttctatag aagagcgaaa
    51961 aacttcagaa atgtaggcag cgctgaccat agaaaaacca agataagcgc aaagtaaagc
    52021 tggcaattct aaaccataat aaggcaaccc gaaataaaga acaaagagca ccacgatcat
    52081 cggcacacct cgcataatag agatgtacgc tctaacaaga taatgaatca acggctgacg
    52141 tgaccttccc agaagggcta ggcctaaacc taaaaacaga cccgtaaaaa aactgagcaa
    52201 cgaaatacca agggtataag gtaatcccga taacacaaat cctaaactat ctttcattaa
    52261 tgggatatta atcatgtggt taggactcct ttaacatcaa gtattagtta acgtctttcg
    52321 tatcaatgac gggaattttt ttgtctttgc caaaaggttt tgtcaaatct tgaccagcat
    52381 agtaggtttc agatattttc ttcaagctac catcttttat catgtcttta ataacagcat
    52441 ctattttttt cttgaggcta tcgtcttttt tgttcataac aattccttgt tcggttgggt
    52501 tgtactgaac atcgctcatt ttgacattta gatttggatt ttgagataac atgtaatcca
    52561 caaataattt ttgagctggg taatcatttg gaatgaaatc tgttcgaccg ttagccacat
    52621 cgtttagata aacatctcct gtaacattat cgtaggtcac cagttcagct ccctgttttt
    52681 gtgctacttt catgtactct gttccagaag cgccggctgc tttttttcct ttccagtcgc
    52741 taagatcttt tttagccatg tttgagctgc catcagcacg gacaatcatg cctcctaccc
    52801 cgtatttata aggcgtagag atattgtatt ttttctgacg ttttggggta atgtcaaagt
    52861 tgttcaaaga aatatccact cgaccactat caactgaggt aaaggcttgg tcaaatcctg
    52921 tttcaacaaa cttgacttta atgttaagtc gtttaccgat ttccttgacc atatctactt
    52981 cgtaacctac taactcatta ttatcgttgt aaaaagaagt tggctgatag gttcctgggg
    53041 tagctacttt taacacccct ttttctttga tagtctccca acttgatttt tgagatttat
    53101 gtggcgcaca tgctgccaat aaaaggctgg caagtccgat agacagtaaa gtaactgatt
    53161 tcttcatagt aactccttta ttaagataag aatagtataa cattttgatt aagttgtgtt
    53221 atcagtgagc gctcaatctc tttaaaaagt taatctataa tagttaaact gttttataag
    53281 attattattg aggaatcttc aataatcctg ctgcctcaaa ctgctctgca attcctaata
    53341 ataggtcctc tctacctttg gctgcaatta gttgaatgcc cattgggagc ccttccttag
    53401 tttcgtaagt aggtaaacta atagctggct ggccagttag attaggcaga gctgtgtaag
    53461 gattaatagc cagactctta ccaaacatgg tttcgaccaa attgaattgt tcttcactgc
    53521 tgaaaatttc agcttgagcc aagttagcca tgagtttcga atctgggaca agttctccgt
    53581 gcttaggagc tggtgtgttg gttgtgaacg tcaacaacag gtcataagtc tcatgaaaac
    53641 tagccatggt tgcactgtac gtatcccatt tttgaaggac ctgtgagtaa cgccaagctg
    53701 ggatgtcttt acctgattgg taaatagccc aggtcatggt ttccatatcg tctttggtca
    53761 tcggacgtcc aaaagtgtct tcaatatcag caaacatagc agccgtttcc acactattca
    53821 taatataata atgacgaatc acttctgtca tatttacagg aaactcttcc aattcaacta
    53881 gctgatgccc ttgttctctt agccaagtta ctgcttgtct taaagctttt gcagtatcta
    53941 aagatactgg gctaccgtca tttaaccgtt gataaaaagc aattgtcaag ggacgttgta
    54001 gcgattgata aatactatct tttgttaagg tggctagggg gaaggggctt tccatttgtt
    54061 ccatctgtaa atagtagagt agatttctag tatctctaac ggatttggtc aaggcaaaat
    54121 gaactgaggc tccttgccag cttcgataag aaccaggtcc gacaggcatg cgcccgcggc
    54181 taggtttgag cccaattaag ccattaaacg aggctggaat ccgaatagag ccacccccat
    54241 cactagctgg tgctagtgca ctaattccag atgacactag agcagcagct cccccagatg
    54301 agccaccagc atttcgtgta ttatcctttg gtaaattcac tggttcgtgc aaactgctat
    54361 cgctaatatt tttaaagcca aattcaggtg tgttagaacg ccctaaaata ataaatccca
    54421 agtcttctaa ccttttcacg aataaatccg ttttagtcgc ttgatactct ttaaacaatc
    54481 tggaacctga ggttgatgag tgacctttta attcttgtcc caaatctttc aaaaaaatgg
    54541 gaactcctgc aaaaggctta cctgaaaaat ccctttgttt ggcttcttct agggctgctt
    54601 caaagcgttc tgaggtaata gcatttaagg tagggttcaa ctttttagcc ttataaatag
    54661 cctgggttac taattctagt ggtgtggtct gaccggtctg aacagcaata gccatagccg
    54721 tcgcatcttg ataagtcata gctactcttc ctttctaaga tatgaatcct aaaacattct
    54781 tttttattaa aacacaaata aaagaaaacc accattaaaa ggttagaaat tagaatgtga
    54841 tgtgtaagaa atgtagacaa aacaatcaaa acataacatt ttgaattggg gttaaagaga
    54901 ttttttcatt aaaaataaac ctggctgata gttccttatt gttgccagtt tacctggtct
    54961 tctttaaatt ttttaagtaa atgtaaacca tcattagtaa tatagccttg caactttgca
    55021 actgcaatta gctcagtata attagaaagc gtaatcaact tgataccagc ttctttaaaa
    55081 ttctgactag cttttggtaa ctcataagtg aaaatcgcaa caactcccag aacgtcagct
    55141 ccctcacgac tggctgctgc ggctgcattt agtaccgaac cacctgttga aatcaaatct
    55201 tcaatgataa ccattttttg gcctttaagc acacgcccct caatctggtt gcctgctcca
    55261 tgatcttttg gcttactgcg aatataagca aaaggaagag tcattttatc tgctataatg
    55321 gcaccgtgag gaatacctgc tgtagctgta ccagcgatga cctcaacttc tgggaaatga
    55381 gccctgatag tttccacaaa cccattctca attaaatcac gagtcttagg ataagaaagg
    55441 gtgacacgat tatcagtgta aataggtgat ttaatgccag atgcccacgt aaaaggatct
    55501 tcaggcttta aataaacagc tttaatatca agtaattgag ttgcaatttg gcttgctagt
    55561 gtcatttctt attctccttt ttcttcttta acagacaagt gttggtttcc taatcttatc
    55621 tgttagcctg cccactcagc ttttatggct tggtaagcag ccactgggtc ttttgcctgg
    55681 gtaattggtc gccctaccac aatataatca cttccaatgc gtcttgcttg gtttggagtc
    55741 atcactcgtt tttgatcgcc aatattactg cctttaggac gaatgccagg agttaagcaa
    55801 gtaaatcccg taggtgtcac cgccttgata gcttctactt cttgtgccga acaaaccacc
    55861 ccatctagct gagctttagc tgcccccttg ctgtaatgca aaacagactc taaaagactc
    55921 gtttgaatat tttgatcacc acgcatttgg tcttcactgg ttgaagttag ctgcgttacc
    55981 gcaattaagg tagggccttg acctagtcct tctcgagctg cttttagcat ctctacacca
    56041 ccagctgcat gaacagttgc catatcaata tctaattctt taagaactgc catagcagca
    56101 cgaacagtat ttggaatatc atgtaatttt aaatccaaaa atacattatg ccctaacgat
    56161 ttgatatacc gaacaatatc tggtccttgc gcataatata attccatacc aattttcacg
    56221 tatagctttt cttctgctgg aaataagtct aaaaatgctt tagtctcctc aaatgatgaa
    56281 aagtctaaag caataattgg gcgttcttct ttcataactt atcctttcta taatgtgtca
    56341 atatctcatt aagcaccaaa aagcctcgct accaaagaag cgaggctaca gcaaactaaa
    56401 agggaattcc ctttttttat cttgtatacc ttgtttgcct ctctggactc acttaaaggt
    56461 gtctaaattt aaactattat agctttttta aaacaagatg tcaaaacttt ttgttatttg
    56521 aacacatctt tcctactata tctttttttg tatacttacg tcaactcatt ttatcatttt
    56581 tacccaatca tttattcaaa ctcacctgtg ggtaccttag ggctttggta atattttata
    56641 aaagcttctt cttcaagatt aaccctctgg atatgttcat agagcacatc cggaatagga
    56701 gcttccttaa gtgacaaagc actcactttt aaagatgaaa aaacatcact taagacccca
    56761 aaaactcgaa tgcgtttatc tccaataata atttgaaaga cttctttgga ctggcataaa
    56821 tctatcagat gatccactcc tttaatccaa ttatcgacaa tatccaaagt tttgttcatc
    56881 aacttaaaag ctgattcttc ataccaaaag gtagactcaa tcaagcgttg ctgcatatcc
    56941 aaggtatcaa tggccgcaac aatatctccc aactcagact gtactaaatg agctgaaaat
    57001 tcccctccaa gtatagaagg agttcgttct acaagcttca gttcatctcg aaaaagagtc
    57061 aaatagtgga tactgaccaa taatttttgg attaagattt gatgactatt ttgttcagta
    57121 acattcacac gaccaccacc ctttgattag aagaatacct atttgttaat actattaata
    57181 taacacaaga aaaataaaag ggcaatgaaa ttgatagatt caactctcaa aattgaatca
    57241 tatttatcct aggccatatt gagaaaagac tccagcgctt tttcaaagtt ctaaacccat
    57301 gatgacgttt gtgcttgcgt ctttattttt tttgagctag actgtactta tgagaattca
    57361 acaactgcat tatattatca aaattgttga gtgcggctct atgaatgaag ctgctaagca
    57421 attatttatc acacaaccaa gtctatccaa tgcggtcaag gatttggaaa tggagatggg
    57481 cattaccatt ttcaaccgaa atcctaaagg gatcaccttg acaaaagatg gggtggaatt
    57541 tttatcttat gctcgccaaa ttatagagca aacatcactg ttagaagacc gctataaaaa
    57601 ccacaatact ggccgagagc tctttagcgt ttcatctcaa cattatgcct tcgtagtcaa
    57661 tgcatttgtc tcactcctca agcggaccga catgactcgg tatgaactct ttttgcgcga
    57721 gacaagaact tgggaaatca ttgatgatgt caaaaacttt cgatctgaaa ttggtgttct
    57781 ctttatcaac gactataacc gagatgtctt gaccaaatta tttgatgaca atcatttgac
    57841 tgcctctcca ttatttaagg cacagcctca tatttttgtc agcaaatcaa atcctctagc
    57901 aactaaaagc ctattaagca tggacgactt gagagatttt ccttacctga gctatgacca
    57961 agggattcac aactcctttt atttttcaga agagatgatg tcccagatgc cccacaacaa
    58021 atcaattgtg gtcagtgacc gtgccaccct ctttaactta atgattggtt tagatggcta
    58081 caccgttgcc agtggtattc taaatagcaa tctaaatggt gaccagattg tggctatccc
    58141 gctagatgtc cctgacgaaa ttgacattgt ttttatcaag catgaaaaag ccaacttgtc
    58201 caaaatgggg gaacgtttta tcgaatacct tctcgaagaa gtaacctttg attaatgtct
    58261 ttttcaatcg tcatactaaa aaaacacggt agtaagacct gtatcggtca agttatcgtg
    58321 ttttttgata aatatcagac aatagattgt ctccaaaggc tcaactcctt tttaataatg
    58381 gcattcatgt catcaaagta aaaaggctca cgtcggtaga ttacaccaga aaaagtcgcc
    58441 aaggtgataa tgttttccaa aaaagcctga gaagaaaaat gagtggtgcg atgcagttcc
    58501 tcttcatcaa aaggcttaat caaatgagcc agaggattac ggacagcctt ttcaaaatgt
    58561 cgtaacaaaa aaaccaagtc cttgatttcc tgaggtaagg aggtcaacgt caacatgtct
    58621 gctaagcttt tggtcgtcac attgcgagac tgcttttgag acaggtaagc cttgaaaacc
    58681 tcgtgatcag attccaacat ggcctgaaaa tgccaggtat catattggtc attctttgta
    58741 tcaaaaatat aagccttgaa gtttggaatt tctgacgtga ttagtctgag aaaaagtcgg
    58801 taaagaatag gcgacactga cctgacgaaa tcaataatag caccgttttt tactttaact
    58861 tctaaatcca aaatgtaatt ggcaatctgc tcatcttcaa gcgattggtt aagccataat
    58921 gaacagtggt actggtcttc aatggtccga ttctctgctg catgttccgc cagaaaggca
    58981 aggttcaatt ctctgcgctc ttttaacatc tctagtaaat agagggcagt tttagggata
    59041 tttggcaagg ctttagcaat tctaaaggcg tgattataag catagacatc caaaaggatg
    59101 tctagtgttt gattattagt cattggcaat taaggtttcc aagccgacct tcatcatatc
    59161 agtgaaagtg gtttggcgct cttcagctgt agtatcttcg gttggatcat tcaagttatc
    59221 tgagatcgtc atgattccaa gggctttgac atggtgctga gcagctaaat agtaaagagc
    59281 tgctgcttcc atttcgatgg ctttaacacc caatttacca agagccatat tacgctcagg
    59341 catgttagtg taaaagacat cagaagaaag cacattccca acgtgagttg ttactcccat
    59401 ttctctggcg atatggtaag ccttgtcaag taaaccaaaa tcagcaattt gtgggaaatc
    59461 aaattctgga aagtcattac ggataatatt tgagttggtt gctgctgctt gggcaagcac
    59521 caattcacgc acatgaactt ctgggtcaat cgcacccgca gtaccaacac ggatcaatgt
    59581 tttgacaccg taatcaacga ttaattcacg agcatagata gagatcgaag gcattcccat
    59641 accagttccc atgacagaaa cgcggtgccc tttgtaagtt cctgtgtagc caaacatgtt
    59701 acgcacttcg ttaaaacaca ctgcatcttc taggaaattt tcagcgataa atttcgcgcg
    59761 caaagggtct ccaggtagaa gaattttatc agcaatatcg ccttttttgg cagaaatatg
    59821 gatagacata aattatgata ctgagggcat taaaagaacg atggcaaaat aggaagttgg
    59881 cagaaaatcc tgattttcta gacaacttat ctttttgacc aagtccttag cccgaattca
    59941 aattaatgaa ctcagccctc acctttctta tttttttatt ttttataagg aagaaaaaca
    60001 gttctccgcc cgtattcaat tcaatgaata cagttagccc tacctttcct tttattttgt
    60061 agaaggtcag agggcggaca gaaagcgact gaaatttagg gaactaacgc catgtgtgag
    60121 ctcaaaagtt agtttatcta gtttccgagc tttccgcccg tattcaattg aatacaaccc
    60181 cattcctttc ataattgata ttgaatagaa gagagctaaa aatcgagttc aaataagaaa
    60241 ggactaactc tctttccttt attatttaaa aattaatacg aagaaatgtt cgagtccata
    60301 ctcaatttaa accgaatacg tctctttccc ttcgttggtt atgttagtta gaaaagctag
    60361 aatctgagtt caacattcat ggaactcaca ctccgtgatt attttttgat ttttttattg
    60421 cgaacagatg tgccaaactg acgacataaa ataacattcc tgtaataatg ctccagttga
    60481 ttttgttcgt cctacaactc cgataaaata gctttaacaa gacctttgaa atcttctttg
    60541 atatgttggg tcacttccac aacttcttca tggttcaatt ctgattgaaa accagctgca
    60601 aagtttgtaa tagctgaaat accaagtact ttaagtccag aatgagcagc cacaataact
    60661 tctggtacag ttgacatgcc aacagcatct gctcctaata ctttgaaagc acggatttct
    60721 gctggtgttt cataagttgg accagttaag cccatataaa caccatcttc tagtttgatg
    60781 ttcatttttt cagccacttc atgtgcctta ttgcgataaa ccttagtata ggcatcagac
    60841 atatcaggga agcgtggacc aaactcgtcc aagttttcac cgattagtgg gttatttccg
    60901 gtcatattga tatggtctgt gattgccatc aaggtacctg ggccgtagcc gataccacca
    60961 gctgcatttg tgacaagaac gccttcacat cccagagctt tcatgacacg aacagggaaa
    61021 gtcaccactt caagcgggtt tccttcgtaa aaatggaaac gaccttgtag ggcaagtact
    61081 ttacgaccag ctaaatcacc gtaaactaac tttccagcat ggccaacaac tgttgattta
    61141 ccccagtttg gaatatctgc ataatcaata acaatagcat tttctacttc ttcagcaagt
    61201 tcaccaaggc ctgaacccag aattaatcca aattcgggag tttcaattcc tttagtaact
    61261 aaaaagtctt ttgtttcgtt aattttagtc atcaatgaca tctttttctc cttttacctc
    61321 aatagggttc acactctcgc catcctcttt catataagga ctaggcaggg gaacaggaag
    61381 aatatccagt aaggtctcag aaggacggca aagttttact cctttgcgag tcacaacaat
    61441 aggacgattg atcaaaattg gatccgtcaa catggcatta ataacgtctt tctcagctac
    61501 tgcttgattt gctaatccat gagcctcaaa ttcaggaaca ttggttcgca agagttctcg
    61561 cgcagagatt cccatcgatt gtaagagctc aattaaagtc tcccgattag gtggcgtctg
    61621 caagtactca ataatagtag gctcaatacc tgcatggcga atcatggcaa gaacattacg
    61681 tgaagtgcca cagtttggat tatgataaat cgtgatttct tccattatca attactaact
    61741 ttcttatttt aagtgaccaa ggaatgactc cccaatcatg gctgtatcaa ctccaaaatt
    61801 ttctgcaaca gtcgctgaaa tatctgcaaa atgcccttgt ggaataagac catttccagt
    61861 aaaggatgca gagtaggcaa gcaatggaat gtattcacga gtgtggtcgg tgcctgcata
    61921 ggttgggtca ttaccatggt cagcagtgat gagaagcaaa tcgtcttctt tcatatttgc
    61981 gatgatttca ggtaggcgat tgtcaaattc atgcaagcag tcgcgataac cctctgggtc
    62041 acgacgatga ccaaagttgg catcaaaatc aacgaggtta gtaaaggaga atcccttagt
    62101 aaactcaggt aattgaagag tcttaataag cgtgtcaata ccatggctgt tcgacttatt
    62161 gtgtcccatg tcgtttgtaa tacctgaacc attaaagatg tcattaatct taccaacggc
    62221 ataagttggg acacctgcgt cagccagttt gttcaaaacg gtatcttgga aaggagacac
    62281 tgcatagtca tgacggttag ccgtacgtgt aaagtttcct ggttcgccta cataaggacg
    62341 cgcaataata cggcctaaaa gggcagggcg ttcaagcgtg atggagcgag catattcaca
    62401 aatcttgtaa agctcttcca caggaataat gtcttcatgg gctgcaattt gaagcactgg
    62461 gtctgctgag gtgtaaacaa tcaactcacc tgtttccatt tgacgcggac caaaatcatc
    62521 aataaccgca gttcctgaat aaggtttgtt ggcctcgcga atgattttgc ggcctgagaa
    62581 ttcttcaatc ttagttaaaa tctcttctgg gaaaccattc cagaaagtgt caaaaggttc
    62641 cgtaatgttc aagcccataa tttcccaatg gcctgtcatg gtatcttttc caagagatac
    62701 ttcttccaat ttggtcacgt aaccagtagg attgtcttct gttggaacag tttttagtgg
    62761 tattggacga gaaatgtttc caagtccaat ttttgccata tttggaaccg ataagcctgc
    62821 tgcttctgaa atatgaccga gagtgtcaga atctgtatca gctacaccag cattaaagaa
    62881 tttgtcagca tctggtgctg caccaatacc gacagagtct aaaacaacca agtgaatacg
    62941 attaaatttt gacataagtc tccaatctag gggaggttac ggttgactcc ccaataccta
    63001 taaaatatat taacgctgtt atatgctaag actagcctgc tgtaggcgct tctaaaacag
    63061 taacgccgtc tttactagca acaatcacct tatcaaccat accattaaag agaccgtgtt
    63121 ccacaacacc aactgttcca tctagcaggt gaccaaaagc aacaggatct ttaatacaac
    63181 ccaaatcaag atcaataata tagttttgca tgtctgtcac caaacgacta tcacctttca
    63241 tacgaaaaga cggtttgtaa ccagcttttt caaagacacg gaacaaacga tcagcaccat
    63301 attgaacgac ttcgactggt aatttaaagg cacccagatg ctctaccatt ttagaagcat
    63361 caacaaccca gatgtattct tttgttggtg ttgcaacaat tttttccatt agtaaggctg
    63421 cgccaccacc cttgattcca ttgaaatcct tatccacttc atcagcacca tctacggtga
    63481 ggtcaatgct atcaatatca tctatcgatt ttaaaggaat gcctaagcct tctgcttgct
    63541 tgctagtcac acttgacgtg gtcacaccaa ccacttgcag tccttcttgc tttacgcgac
    63601 gaccaatttc ctcaacaaaa tagtaagccg ttgacccagt tccaagccca atggtcatgc
    63661 catcagtcac atattgcgca gcggtaacac cggcaatttt tttgagtgct tccatgattc
    63721 ccccttataa tagaattgcg tcttagcact ttattatacc atgaaagcgc caacttgtta
    63781 atgaaaacat ttgtttttct ctcattgaca gtctaagtgc tatagaatag gatgatcttt
    63841 ttaaaactta tgatttattc taaagtcatc atataagcac ttacttttcg tgtttattta
    63901 gctttttgaa aatcaagtgt ttttgtttga cctttactga ccttttgatt ataataatac
    63961 tataagatga gtaaaaataa tgtctgaatc attttattag caaagggttt cctatcatgt
    64021 gaaatcctaa caacgattct gcgactgctt acaaacatct tatagtctat tttttattgc
    64081 gaggtaactg atatgacaca tttttctggt aaagatcctt ttgtaaatat ggatgatatt
    64141 ttcaaccaat taatggcaaa catgggtggt tatcgtagcg aaaatccccg ctatctcgtt
    64201 aacggtcgcg aaataactcc cgaagaattc caacactatc gtcaaacagg acaactccct
    64261 gtagcgacaa ccaaagcaac aaattctcaa atgttaacac ctaaagctga tagtgtattg
    64321 actcaactcg gaactaactt aacacaagaa gctcgtcaag ggcatcttga ccccgtcatt
    64381 ggtcgtaaca aagaaattca agataccgct gaaatcttgg cacgacgaac taaaaataat
    64441 cctgtccttg tcggagatgc tggtgtcggt aaaactgcag tcattgaagg tttagctcaa
    64501 gccattgtta atggagatgt cccagctgct attaaaaaca aggaaatcgt atcaattgat
    64561 atttctagtc ttgaagctgg tacacagtat cgtggttcct ttgaagagac cattcaaaac
    64621 ttgattcaag aagtaaaaga agctgggaat atcatcttat tttttgatga aattcatcaa
    64681 atagttggtg ctggtgccac aagcagtgat tcaggctcaa agggacttgc agatattctt
    64741 aaaccagcct tgtcacgagg agaattaaca ctgattggtg ctactactca agacgaatac
    64801 cgcaatacta ttttaaaaaa tgctgctctt gctcgtcgct ttaatgaagt taaagtgaat
    64861 gcaccatcag ctgaagatac ttttcacatt ttaatgggta ttcgtaatct ttatgaacaa
    64921 caccaccata ttacccttcc tgataatgtt ctaaaagctg ctgttgatta ttctattcaa
    64981 tacatcccac agcgtagcct gccagataaa gcaattgact tactggatat gacagctgct
    65041 catttagctg cgcaacaccc ggtcactgac ttaaaaacac tcgaaactga aatagctaaa
    65101 caaaaagaga gtcaagaaaa agctgtcgcc aaagaagact ttgaaaaagc tttagcagca
    65161 aaaacacgga ttgaaacatt acaaaagcaa attgagcaac ataaccaaag ccaaaatgtt
    65221 actgctacag tcaatgatat cgcagagtct gtagagcgct taactggtat tcccgtctct
    65281 aacatgggga ctaatgattt agaaaggctc aaaggaatta gcagccgctt aaaaagccat
    65341 gtcatcggac aagatgaggc tgttgcagca gtagcacgtg ctattcgacg aaatcgagca
    65401 ggttttgatg atggtaaccg tccaataggt tctttccttt ttgtgggtcc aactggtgtt
    65461 ggtaaaacag agttagctaa acaattagca cttgatttat ttggttccaa agatgctatt
    65521 attcgacttg atatgtccga atataacgat cgcactgctg tgtcaaaatt gattgggaca
    65581 acggctggtt atgttggcta cgatgataat aataataccc taactgagcg tgttcgtcgt
    65641 aacccttatg ctattgtctt actagatgaa attgaaaaag ctgatcctca aatcattaca
    65701 cttcttctac aagtgttaga tgatggacga ttgacagatg gacaaggaaa tactattaac
    65761 tttaaaaata ccgttattat tgctacatca aatgccggtt ttggccaaca agatacggaa
    65821 acttctgggt ctaacattat ggatcgcatt gcaccttatt tcagaccaga gtttttgaac
    65881 cgttttaata gtatcattaa atttaatcat ctgcaaaaag aaaatttaga agaaattgtt
    65941 gatttaatgc tagctgaagt aaatcaaacc accgcaaaaa aaggtattag tttaacgatt
    66001 gctgatgacg ctaaagctca tttgatagat ttagggtaca atcacgctat gggagctcgt
    66061 ccattacgac gtattatcga acaggaaatt cgtgaccgta taactgatta ttatcttgac
    66121 catccagaag tcaaaaaact tcaggcaatc ctaaaagaag gacaactagt gattaagcaa
    66181 aaagatcagt aataaacttc ttcaattgaa taattttcaa aacatgaaaa aatccgtctt
    66241 gtgactacgt tatacacttg acggactttt ttttagcgct tttttaaaaa taattttttc
    66301 atcgcctaat aactcaagtt tctttccaaa tctggtgtaa tcaaaataac cattaggtgt
    66361 gagcaatcct tttgaaaaag aagtatgtcc cgctgctact tcttctttga tatcaatggt
    66421 gtgcaaaata cgattaactt ttcctttgag aacaacagca gataaatgtc ctgttttttg
    66481 attatatttg cctccgatct gagaagctaa tttctcaaaa gcttgatcta aaatcatttc
    66541 taattcatgg ctagctggca tactattctc aataatttgg cgttctttac tactcaagtc
    66601 accaacaact aaatgatttt cctttaatgt ctttaataat tcattttggt gataagcagc
    66661 taaacgtttg acaaaaatgt ctcgttcaac ttgaacacta aaactcaagt caacgttgtt
    66721 atttttaact gttagtatca tctgagatga cgttaataaa ctatcatcaa taagagggct
    66781 ctgatgcaaa ccagtaagac gtttagcaaa atcatcgcct aacttctgat caatgcttgt
    66841 tgttcgccag ataccttcaa tagagctatt acgatatcga acaccaaaaa gtacagcaag
    66901 caagataaga aaaataataa atacaaaaag ggcattgcat tgatggcata acaatgactt
    66961 tcctttagag cgcccttggg ctacttgtgt catgactcat ctccttaata taaaatacta
    67021 gcatgtcatc attaaggcgt caagtaaaat cggtaaacct aactatgatt ttttatgttt
    67081 tcgataagaa aaacaccctc tactgagaat gttttagcat agataaatca acacctatcg
    67141 ctagacctgc tctaaaatag catcccaaga atcatcgata ccaatacgtt ctaccgaaga
    67201 aaatactata aaagtatcag atttgtcaaa attcaaagct ttcttgacca cagactcatg
    67261 tttgttccat ttgccacgag gaatcttatc tgctttggtt gccacaacaa taacaggaat
    67321 gtcatagtac ttcaaaaagt catacatctg aatatcttct tttgaagggg catgacgtaa
    67381 atcaaccaaa ctcacgacag cacgcagatt atctctggtg gttaagtatt cttcgatcat
    67441 ctttccccat ttggcccgtt cacttttaga aaccttggca tagccataac cagggacatc
    67501 cacaaatcta agcttgtcat caatattgaa aaaatttaat agttgggttt ttcctggctt
    67561 gcttgaggtt cttgccaaat ttttgcgccc caatatggta ttaataaaac ttgatttacc
    67621 aacatttgaa cgtccagcga gggcaatttc tggcaaatca tcttgggggt aatgagattt
    67681 attggcagca ctcaataaaa ttgaggcgtt atgggtattt aagacttgtt cttcagccat
    67741 tatttctcct taagctgtct ctaaaacggg ttttgacttg ccttcgacag cagccttggt
    67801 gatacgcact tttgtgacat cttcttggct tggaatttca aacataatgt ctaacatcgt
    67861 ttcttcaata atagaacgaa gaccacgtgc tcctgtctta cgttcaatgg ctttattggc
    67921 aatggcttct aaggcttcct tatcaaatgc caattcgacg ccatcgtaag aaagaagggc
    67981 ctgatattgc ttgaccaggg catttcttgg ttctgtcaaa attttaatca aatctgatgt
    68041 gtttagttgt tcaagggctg ccacaactgg taagcggcca ataaattctg gaataagccc
    68101 aaacttttga atatcttctg aaataatttc ttgcatataa gaagcattgt catcaatttt
    68161 acggctgttt tgaccaaaac caataacttt ttcacctaaa cgttgtttaa caatctcctc
    68221 gatgccatca aaagccccgc caacaataaa caagatattt tttgtatcaa tttggatcat
    68281 ttcttgatta gggtgcttcc gcccaccttg tgggggaaca cttgctaccg taccttcaat
    68341 aattttaaga agggcttgct gtacaccttc accagataca tcacgtgtga tagatacgtt
    68401 ctcacctttt ttagcaatct tatctatttc atcaacataa ataataccac gctctgctcg
    68461 ttcgacatta tagtcagcag cctgaatcag tttgagtaaa atattttcca catcttctcc
    68521 aacataccct gcttcagtca aagaagttgc atctgcaata gcaaatggta cattgaggct
    68581 tttagccaga gtttgtgcta agaaggtttt tcctgaacct gttggtccaa tcatcaaaat
    68641 gttggacttt tgtaaatcca catcatcgtc atcacgactt tctgtaaacg aaacacgctt
    68701 ataatgattg tacacagcaa ctgataaagc acgtttagca cgatcttgac caaccacata
    68761 ttgatttaaa acatcaagga gctcttttgg ttttggaact tctgtcaaat cggcaaggac
    68821 ttcttcagcc aattcttctt ttataatttc ttgtgataaa gctacgcatt cattgcagat
    68881 gaaaacatta tttcctgcga taattttttt aacgtcatcc tgacttttac cacaaaatga
    68941 acagtatact ttaatatcgt tagttctact tcctgccatt tcttataatt ctcttacttt
    69001 ctcttatctt ttactgaatt ttaatattag tcaatgtgtt gtattggttt ttggtgattc
    69061 ttgcttgata gctaaatctt caccagtgct gtctttgcaa gtcacatctc aaaacaatgt
    69121 accatagaag gcgtgaatac tattaaccag attagtaaac gcatttaata agaacagact
    69181 tcctaagcca taacgattct ttttaaaagc tgggatggca caaagaacac aaatcacgca
    69241 caaaaaagcc gtaaatactc caaaaatact acgttgcatc gcttatcatc tcctcagact
    69301 atttgtgttt taaagcagta acggtaaaat cataaagatt tttagcgtct cgagcataga
    69361 acgtttggct ttcttgcgtg aaatgactaa aatctagatt tggtcggtag gtatctcctt
    69421 taaaacgatg atggataact gtttttatga tacgatcgaa ataaccatta aacgcttcta
    69481 aaaccttatt cccaccaatg atataaagtg ttttatcctg cgcatggtac cactccaaga
    69541 ctttttcaat actagtcatt gtcaacacct catctacttg ataatctcta tttcttgtca
    69601 tgacaagggt ttgacgttga ggaagtcttt tgcaattcat tccttcaaaa gtcatacggc
    69661 ccattaagat tgcttgatgt agcgttgtct ttttaaaatg ttctaactct tttggtaaat
    69721 accatggtaa cttacccgca acaccaataa gccctgcttc atcctctgcc caaatagcaa
    69781 taatttcctt tgtcattaat tatttccttc attttaaact ctatttattt taccaaattt
    69841 atgaacaaaa tgctcacaaa aaatctaaat aaagtttaaa tagcaaggtc aaagtgcaac
    69901 tgaggcttca ctggatcata attttgtaat tcaaaatcat ctggtttaat atcaaagaaa
    69961 ttagtcccat ctggaacatt taaaacaagc ttaggctgac tggcaactgg ctgtcgtttt
    70021 agtaattctt gagcctgatc aaattggttg tcataaatat gtaaattgtt aacaaaataa
    70081 aagaacttac ctattttcca tccaaaatgt ttagcaatca tcatctgcaa ggcaacgtat
    70141 tgcatggcat tgatgtgatg agcaactaat atgtcattag aacgttgcgt caaactagca
    70201 tctaaataaa gatcttcccc tacacgtcta acatcaaaca tgatttgaaa ggcacatggc
    70261 aacaaccctt ttgtctcctc aaatgcttca tagtcccaga gagaaatgac attacgacga
    70321 ttccaaggat tttctgctaa ttgctttagt atttttgaga tgatatcatg ttttttgact
    70381 acagcaccat aacgttgccc aatagtacgt gtctggtcaa cttcccattc attccaataa
    70441 tgaacattat atttagcttc tagcacatct aaactgttag actgatcttg atatatccaa
    70501 aataattcct ttatagcaga tttaattgga atgggtcgta aagttgtgat gggaaactct
    70561 cctttagcta aatcatattc agcaaacgcc ccagtaatat acttagaatg agctgttcga
    70621 ccatctttat atttaggacg tgcttgctca ctcaatgatc cttcatttat aattttttga
    70681 atattagctt taaaaatctg gtcagctttt gtcatctcta ataacctttc tgtaagcata
    70741 cttcatacta ttctaactaa ttttaccaaa aaataccaga ataaataaac attcgaaaaa
    70801 cttcatgtgg tcatgaacat tttaactatt aaaggataaa acaaataact ttatcagtct
    70861 aattttgcag taaaatgtga tagaatgggt aagactataa aaataactaa aagaaggaca
    70921 aatatgacaa ttggaattga taagattggt tttgcaacca gtcaatatgt cttgaaattg
    70981 gaagatttag cgcttgcacg ccaagtggat ccagcaaaat ttagtcaagg gctactcatt
    71041 gaatctttta gtgtggcacc aatcactgaa gacattatta ctttagctgc ttctgcagca
    71101 gatcaaatct taaccgacga agatcgagct aagattgata tggttatttt ggcaactgaa
    71161 tcgagtactg atcagtcaaa ggcatcagct atctatgtac atcacttagt tggtatccag
    71221 ccttttgcac gttcctttga agtaaaacaa gcctgctata gcgcaactgc tgctctagac
    71281 tatgctaaac tgcatgtggc ttctaagcca gattctcgtg tccttgttat tgctagtgat
    71341 attgctagat acggtgtagg atctcctggc gaatcaactc aaggatctgg tagtattgct
    71401 cttttggtaa ctgctgaccc tcgtattctt gctctaaatg aagataatgt ggctcaaact
    71461 agggatatta tggacttttg gaggcctaac tatagtttca caccttatgt tgatggtatt
    71521 tactctacca agcaatatct caattgctta gaaaccacgt ggcaagctta tcagaaaaga
    71581 gaaaaccttc agttatctga ttttgctgca gtttgtttcc atattccatt tcccaagtta
    71641 gccctaaaag gtctaaataa cattatgaat aacacagtac ctcctgaaca cagggaaaaa
    71701 ctaatagaag cctttcaagc ttctattact tatagcaaac aaattggaaa tatttatact
    71761 ggctcacttt atctaggatt gttatcttta cttgaaaata gtaaagtatt acaatctgga
    71821 gataaaattg gtttttttag ctatggttcc ggtgccgtaa gtgaatttta ttccggtcag
    71881 ttagttgctg ggtacgataa aatgttaatg actaatcgac aagctttact agatcaacga
    71941 acacgtcttt ccgtttctaa atacgaagac cttttctacg aacaagtcca attagatgat
    72001 aatggtaatg ccaattttga catttactta actggaaaat ttgctctaac agccatcaag
    72061 gagcatcaaa ggatctatca taccaatgac aaaaactaat cttaactgga gcggtttttc
    72121 aaagaaaaca ttcgaagaac gcctccaact tatcgaaaaa tttaaactac ttaatgctga
    72181 aaacttaaat caactcaaaa cagacgttct tttgcctatc caaacagcta atcaaatgac
    72241 tgaaaatgtc ttaggacgat tggctttgcc ctttagcata gctcctgatt ttcttgtcaa
    72301 cggttcaact tatcagatgc cttttgtcac ggaagaacct tctgttgttg ctgcagcatc
    72361 tttcgcagca aaactaatca aacgctcagg tggttttaaa gctcaaaccc taaaccgaca
    72421 aatgattggt caaattgttc tttacgatat cgaccaaata gataacgcta aagccgccat
    72481 ccttcataaa acaaaaaagc taattgcatt ggcaaataaa gcttatcctt ccattgttaa
    72541 aagaggtgga ggcgctagaa ccattcattt ggaagaaaaa ggagaatttt tgattttcta
    72601 tctgactgtt gatacccaag aagctatggg agcaaatatg gtcaatacta tgatggaagc
    72661 tcttgttcct gatttaacaa gactgtctaa ggggcattgt ctaatggcga ttttatctaa
    72721 ttacgcaaca gagtcgcttg ttactactag ttgtgagatt cccgtgcgcc ttttagatca
    72781 cgataaaaca aaatccctac agttagctca aaaaatagag ctagccagcc gactagctca
    72841 agtagatcct taccgggcta ctactcataa taaaggtatt tttaatggca ttgatgcagt
    72901 ggtaatagcc acaggaaatg actggcgtgc tattgaagca ggggcccatg cttatgcctc
    72961 aagaaatggt agctatcaag gacttagtca gtggcatttt gaccaagata aacaagttct
    73021 gcttggccaa atgaccctcc ctatgcctat tgctagtaag gggggatcta tcgggcttaa
    73081 ccctactgtt tctatcgcac atgatcttct taatcaacct gatgccaaaa cattagccca
    73141 attgattgca tctgtggggt tagctcaaaa ctttgctgca ctaaaagctc tgacctcatc
    73201 tggcatccaa gctggtcaca tgaaactaca tgcgaaatca ttagctcttt tggcgggggc
    73261 aacccaagac gaaattgctc ctttagttaa tgctttacta gctgataaac caataaatct
    73321 agaaaaagca catttttact tatctcagct aagacagtct tagatttaaa caactaatat
    73381 aaggcaatca cccatgtaaa ccaattgttt acatgggtgt ttaatctttg tttctataca
    73441 tacaagtctt agaaatagtc tccataacat actctattta ctcatctact ctattttgag
    73501 tttgatgagg tatttataaa tattttatat tatgttctaa aaaagctcta tcatctttgt
    73561 agtaggtgac actattgtag aggtgatgaa gcctaattga cctgctgcaa gcgtccatat
    73621 agtaagtagt cgactccctt taattcttta atagttttac agtcaagagc acacataatg
    73681 atttttaatt cttctttcca gccgttaacg atagcaatca cacgctcggt tgggtatttt
    73741 tcgaccaatt ctaaaacggt gcgtgagagt cccactgcac gtgctcctag gacgaaacat
    73801 ttaatcatgt ccaaggggtg tctgacacca cccgaagcta agatttccac ttggtccatc
    73861 agtccttgtg catttagcaa gcactgaacg gtggtttgtc cccaatcgtt taagtaagag
    73921 cggtctcctc ctctttgatt ttcaatatag gcaaatgagg tccctcctct tcctgaaata
    73981 tcaaaggttt gaatgcctag gtcatgtgct agcttgatac tattgacatc catgccaaaa
    74041 ccaacttctt tgagaatgac aggaactggt atttgactag cgtattcagc aaggtgtttt
    74101 ttccaggtat gaaagacgcg ctcaccctct ggcatcagca actcttgcat cacattcaca
    74161 tgaacctgta aaaagagggg ctgcatttct tgaaccgttt gttgtcctag cgccacaggt
    74221 ttatctagac caatattcgt ggctagtttc aagttatctg ccacctcatg taaacgatag
    74281 gaatcgtcgt taggattttt taaagcagcg ctataagacc ctgtcaccat gacaatccct
    74341 gttgctgctg ctactttggc caatttttca ttgacagctt tgcctttttg acttcctcct
    74401 gtcatggcat tgatgtaaaa gggaaagtcg aagtcttgcc cggcaaaatg agtactgaga
    74461 tcaatatcag acaaatcata gctaggtaag gaatgatgta tgagttctat gtcatcaaaa
    74521 gcattataag gcgattggta cttgagggca tatttgatgt gatcatcttt acggttggtc
    74581 attctctccc cacctttctt gataaagtaa tgcgatatca gcttcttgcc atttggaaat
    74641 aagggtatct ctagcatcct gattaaaggc aagcgcgatg ccacaatctc caccaccaga
    74701 acctgaagat ttagcaacag catcttgctt ctgacaagca gctaccaagg ttactagctt
    74761 aggatggtag atagctggat gaagctcttt taggagatga cttgctcctg ctaaactttt
    74821 cttgagttct tccttatgac cttcttgcaa agctaccata gctgattgcg ttagctggta
    74881 acttgttctt aagaaagctg gggtaatgct cgctgtcact tgttgaatca tctgccttga
    74941 aatagcaggt attttagtcc agccaaccaa aaagtcacaa ggcagggctg gttgaatgac
    75001 ttggatatgg taaccccaat ctttgacgag aagttttttt aagggcatgg tctgcagcca
    75061 gttactgacc tgttctcggt caaaggaggt ataggcgact aaagtctggt aagcgataca
    75121 ggcaatatcc cccatagacc cattatctcc ttgcttaagc aaggtataag ctgctagttt
    75181 aaagagtaac tctggggtta aagtgatctg ataataggcc gataaggcct ttaaggttaa
    75241 gagggtgacg ctaccacttg aaccaatgcc aaattttttg ccatcgcgct ccatttttcc
    75301 tgttatgatt agggaaaatg gctccagttg gtcaattgac tgtcctagat aatcagcaaa
    75361 ggttttaacc gttgcttgaa tcagtgcata agaggcatcg ggtgtcatgc ccgctttatg
    75421 ggaaaacata tctgaagcta attgaatatg ggctgctggg ctaatttctg ctgtcatcat
    75481 cagtggaata aagtgaatta aggctttttg tcctggtatt aagatagcat attcgcctgt
    75541 gaggtatagt ttcccacctg tttgcacaca ataattagac atctggtaaa tcctttgttt
    75601 ttgaaacgat aatacggtag tttttgccaa gtcgttctgc tagttgagcc aagtcttttt
    75661 ctaaacatag gaccttcaca tttggacctg cgtccatggt aaaataacaa gcaaaacctt
    75721 cttgacgcag ttctttcaca gcctccatag cctggtaaga ctcttttgtt aggtaagaaa
    75781 agggaggatt ggctgtcttt gtcgtggcat gcatggccaa ggcattagct tctgttaact
    75841 gaccaacttt ctcaaaatta ttagttttga gataggtaag catatgttgg taatcgattg
    75901 ccgattgttc tacccattca tcaaatgtag ttgaggtatc gcggcataac ttcatgccct
    75961 ctcggctaga aattggcttt ttcgcagcat ttaagaccag cataatcatg gccattttca
    76021 agtcagtctc aaccttgtaa atagcaccac tatctttgtc ccaagcagca actgggccaa
    76081 aaaaagaacg agaagatgat cctgaggcga acttggcctt ttgtgctaaa gctttctgat
    76141 ctagctgagt gtcaaataat tgatcacagg ctttaaccaa ggctgataag ccactggaac
    76201 ttgatgataa acctgcagct gttggcatat tattttgagt ttccatcttt acaaaagcct
    76261 gaccaggttg gcgaaattgg tcaatgatag tagaaatttt ggtatgttct tcgtcatttt
    76321 gcaagacacc gttaatgtaa aactgatcac tggttgcagt atctggtaag aaggaaacgc
    76381 tggttgtggt gaacatgttt tccaaagtca aagaaatgct agaggtcgaa ggaatcatct
    76441 tagcttgatt ctcttttccc caatatttga taatagcaat gttagcatag gatgttacgg
    76501 ttataacatt tggatccacg tgtttacggc tccttcttct tttaatctgt gactgatttt
    76561 ttcggccatg tctttggtat ctgctagggc aatcatacag ccacccaagc caccgcctgt
    76621 catcttagcg cctaaagcac ctgctcttag ggcagcctcc acaagttggt cagctttact
    76681 gatgctaacg ccaatggcct ttaaagcaga gtgcgcttgt gtcatgagct gaccgatagc
    76741 caccttattt ttttggttaa tcgctctttc aagggcttgt gtcaaagctc ctagtttagc
    76801 cagatagggc aaattcgttt cctcaaactg tgctacctta ttgacggcct cacgtgtatg
    76861 accgtgaatc cctgtatctg caatgataag ataaccattt aaggcgattt cgatagtttc
    76921 aaagccaatg tttcggataa acttaatggc atggtcacta aggcatgtct tggcatctaa
    76981 accactagga ttggtgtggg caataatttc tgccttgttg actaaaattt ccaacaaatc
    77041 atccgagagg ggttcttgac aataagaaaa aacggctcta atagctgcaa tagaaacggc
    77101 tgccgaagat cccataccac gcttttgtgg cacttgtgaa acaatctcat aagcaattgg
    77161 ttcttgtagt cgctgcaaat aatccaaggc agcataaatg gctgttgata gagtatcata
    77221 aaaatcaaag accaatggtt tatcagctgg aaaaatatga caaaccacct caatatctgt
    77281 taaaggcaaa gcaatagctg ggtagccata cacaacagca tgctcaccta tcaaaatgat
    77341 cttactgtgt gccttaccat atccaatgtt ttcgttcatt ttttatcttt tcattactaa
    77401 ttcttctaaa cttttacctt actattttac actaaatgat tagaaaatgc gatgatatat
    77461 ctttttaaaa tcatttcttc accgaaatta acaataaaga ctatctactc tttttaggat
    77521 cataacaaaa attttcttat ttcaaactac cttctttaat cactcttcta ctaaattaaa
    77581 cgttaaaaac cagcctcatg ggctggttaa gcatcgttta ttacctttac ctagacatta
    77641 ttactagcta aaggtactga aaaatgttta agtcaaatca ccactgtaaa aacaatagca
    77701 tcgtttttat aatttacagt tatcgttcct ttaaataact taaccatact ttctgccata
    77761 gataaaccaa ttccataacc tttttctttg ctattatggg attcgtcttc gcgataaaag
    77821 cgttcaaaaa agcggctgta atcaatggat tttccttcca aataggtatt agaaacttct
    77881 aatttcgctc gttttcgtct tcttccaata gtggttaaag ataccttgac caagccttta
    77941 ggatcacaat atttattggc attatcaact aaaattgtca caagttcaaa taatgacttt
    78001 tcctccgctt taatcataat attcggctga atcgtcaaat caaaacgttt accatctttc
    78061 aaaaccagac tcttaaaatc ttcagctgca tcttgagcaa tagctgaaaa gtctaccata
    78121 tgtaatacca catctggctg ttcctccaaa cgagccaaag taatcatttg attaatcaag
    78181 cctgtcaatc gcttcacctg atcacttgta ctcttggtcc attctgattc tccagtcatt
    78241 aattcgacca attcattatt ggctgagata atggctagtg gtgtttttaa ctcgtggcct
    78301 gcattagtaa taaagcggcg ctgcttctca taattttgga caaaaggttt gatgaccata
    78361 ccggaaaaaa gactcactaa aacaacaaaa aagataaatc ctccaaaggc caacatcatt
    78421 gaaacagcta ataaatctcc tacactacga aaataaaagg tagtatctaa aataacaacc
    78481 agtttttctt catttggcag ctgagtaatc aagtacgaat aaacactatc ttggtgacgg
    78541 taactgcctt tttcttcccc agacttagca aaaagtctag caaaatattg ggcttcttct
    78601 ctatctaaag ctgaaatatt agcagtatta gaagaaagta gatgtccatt agcattaaaa
    78661 atgacactat aataccgaaa ctggcttagg ctatcttcag acagtttcgt tccaagtcgt
    78721 ttagagcttt ccgtagtgtc aggtaattta cctttatttg aggaaattaa atgtaaaata
    78781 cggttaattt cttgttgact ttggtaacag cgtgccgtgt taataatacc aacaattgaa
    78841 cttaaaataa taaaaatggc aatagaagca atcatgataa aacgaatacg gatacgatta
    78901 aacatgtcac ctcctatttt tctaataaac aatagctagt atctttatca cctgcaataa
    78961 tgacatttgc ttgaatagct aataattttt gacgaagata agaaatataa acccaaacaa
    79021 tagaagtatc ataatcttct tggtctgacc aaacatgttg gtaaagtgtt ttagtatcca
    79081 agtactttcc ttgatttaac attaaaaagg ccattaattt accttcttta gaagctaatc
    79141 gtatagcatt accggcactc agttcttgct cattaatatt taatgtgaca ccagcaaatt
    79201 gtaatacctg aggagtaaaa ctttctactc gtctttccat cgaccttaat ctagctaaaa
    79261 gttcttttaa tgagaaaggt ttagtcagat aatcatcagc tcctgcatct agtccggtaa
    79321 cacggtcatt aatttctgcc atagccgtca gcataataat atgtgagcaa tttcctgaag
    79381 cgcgaatctc ctttaaagcc tcaatgccag acttaatcgg catcatgata tctaaaatca
    79441 taatatcata tgcattgtct ttggctttat caattgcctc ttgaccgtta aaaaccacat
    79501 caacgtcata accttgatga gtcatggcag ttgtcaaaac attagacatc tgccattcgt
    79561 cttcagctaa taaaattttc atggttactc ttcctccgtc gtttcttgaa tcaattgacg
    79621 aatggtttgc atggtaacat cacaagatgc caactcgtct gcacaacgct ttaactctct
    79681 tgtaatcatt tctgcattag caacttgatg tttgtacttc atttgatgtt ccaagctagc
    79741 ccaagagtct tgagcaatgg ttcgcaactg cacttctata aaataacatc cttttttttc
    79801 ccctaaaata tctggaaatt gtgtttctaa ttccaaaatg agatgatatg atctatagcc
    79861 attaggcttg gcatttaaaa tataatcttt ttcagtgtta actgacatac caggaatgct
    79921 tttgagcaag tctaccatcc gataaatatc atcaataaaa ccacaaataa tgcgaatacc
    79981 gatactatct ttaatgattt ttaacgcaga tttgcttgtt agaggaagct gtttgcgttg
    80041 acatttttca atcatcgaag cttccgattt aatacgggat gtaaagtgtt cgtatagttt
    80101 aaaccctgtt tcttttttgc ttttgatatt ctcagcaata atcacatctg ttattgtctg
    80161 tagtaccaac ggcaagtaca cttcaaatcc gctatatata gagctggtag aataatgttc
    80221 tagtctttct ttcatacgct tattataaca aattttctca acaaatgctt aaatctctgc
    80281 aatgatcggt tatctttatt t
//
LOCUS       DAUGFP010000003        75954 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.003, whole genome shotgun sequence.
ACCESSION   DAUGFP010000003 DAUGFP010000000
VERSION     DAUGFP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 75954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75954
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.003"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            75..2594
                     /gene="secA"
                     /locus_tag="VH663_000155"
     CDS             75..2594
                     /gene="secA"
                     /locus_tag="VH663_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEQ8173390.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMASLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVNEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAVDAIVTFARTSLVPEESISAKELRGLKDE
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
     gene            2710..3066
                     /locus_tag="VH663_000156"
     CDS             2710..3066
                     /locus_tag="VH663_000156"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEQ8173391.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWSGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            3063..4163
                     /locus_tag="VH663_000157"
     CDS             3063..4163
                     /locus_tag="VH663_000157"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEQ8173392.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKEVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLPKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            4250..5779
                     /locus_tag="VH663_000158"
     CDS             4250..5779
                     /locus_tag="VH663_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ8173393.1"
                     /translation="MDKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSDNKA
                     EAENQVDDKSTDHSKSTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSKQLPEARTPI
                     QSLSPYVSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKS
                     TGIAYDSTRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAF
                     DLDPTQASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFT
                     DTTGSGKRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLG
                     YQASSYAWGQCTWYVYNRAKELGYQFDPFMGNGGDWKYKVGYALSKTPKVGYAISFAP
                     GQAGADGTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSK
                     N"
     gene            6270..10097
                     /gene="shr"
                     /locus_tag="VH663_000159"
     CDS             6270..10097
                     /gene="shr"
                     /locus_tag="VH663_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEQ8173394.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEPLVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKPVPQN
                     QGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDITITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLEKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     IEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKASNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVENGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVPYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENS
                     HLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKVTGQSTQETSKTDDTDKA
                     EKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIV
                     ALGFIIGRKTLFK"
     gene            10294..11169
                     /locus_tag="VH663_000160"
     CDS             10294..11169
                     /locus_tag="VH663_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEQ8173395.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTNLVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDSQKPVDTKLGESQDEPHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            11153..12037
                     /gene="isdE"
                     /locus_tag="VH663_000161"
     CDS             11153..12037
                     /gene="isdE"
                     /locus_tag="VH663_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEQ8173396.1"
                     /translation="MIKRCKGIGLVLMAFFLVACVNQHPKTAKETEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSVNPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHP"
     gene            12039..13061
                     /locus_tag="VH663_000162"
     CDS             12039..13061
                     /locus_tag="VH663_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ8173397.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYEAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYALAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            13058..13894
                     /locus_tag="VH663_000163"
     CDS             13058..13894
                     /locus_tag="VH663_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173398.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQIWLEQEELATISIKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDIQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGQLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNGIRTQRRDRNK
                     NR"
     gene            13891..15654
                     /locus_tag="VH663_000164"
     CDS             13891..15654
                     /locus_tag="VH663_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEQ8173399.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLPLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQATERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLSQETINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLNTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            15647..17317
                     /locus_tag="VH663_000165"
     CDS             15647..17317
                     /locus_tag="VH663_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173400.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGIYHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     EACGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LYEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGFLKEVNKK"
     gene            17314..17907
                     /locus_tag="VH663_000166"
     CDS             17314..17907
                     /locus_tag="VH663_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEQ8173401.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPILLMWLLKAAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSVFAHYLLKKHFIKSGLLS"
     gene            17904..18584
                     /locus_tag="VH663_000167"
     CDS             17904..18584
                     /locus_tag="VH663_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ8173402.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            18521..19975
                     /locus_tag="VH663_000168"
     CDS             18521..19975
                     /locus_tag="VH663_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173403.1"
                     /translation="MERLGGADLDYCYHNHHDTSMISAEQLVFTYHDQKNPACQVPTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQAGRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQNLLALTDEQQQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            20350..22365
                     /gene="recG"
                     /locus_tag="VH663_000169"
     CDS             20350..22365
                     /gene="recG"
                     /locus_tag="VH663_000169"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEQ8173404.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPDTLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAALKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSVIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     HKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAKNIRKKEIYD"
     gene            22658..23560
                     /locus_tag="VH663_000170"
     CDS             22658..23560
                     /locus_tag="VH663_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ8173405.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSNISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIHEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
     gene            23573..24277
                     /locus_tag="VH663_000171"
     CDS             23573..24277
                     /locus_tag="VH663_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173406.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(24626..25591)
                     /locus_tag="VH663_000172"
     CDS             complement(24626..25591)
                     /locus_tag="VH663_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEQ8173407.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            25663..27051
                     /locus_tag="VH663_000173"
     CDS             25663..27051
                     /locus_tag="VH663_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8173408.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGVAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDVEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            complement(27174..27626)
                     /locus_tag="VH663_000174"
     CDS             complement(27174..27626)
                     /locus_tag="VH663_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEQ8173409.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNIKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            27857..29071
                     /locus_tag="VH663_000175"
     CDS             27857..29071
                     /locus_tag="VH663_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ8173410.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISMSLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDGK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            29288..30070
                     /gene="codY"
                     /locus_tag="VH663_000176"
     CDS             29288..30070
                     /gene="codY"
                     /locus_tag="VH663_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEQ8173411.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDDEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            30217..30771
                     /locus_tag="VH663_000177"
     CDS             30217..30771
                     /locus_tag="VH663_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEQ8173412.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSYMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            31215..31533
                     /locus_tag="VH663_000178"
                     /pseudo
     CDS             31215..31533
                     /locus_tag="VH663_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dikinase"
     gene            31765..32067
                     /gene="gatC"
                     /locus_tag="VH663_000179"
     CDS             31765..32067
                     /gene="gatC"
                     /locus_tag="VH663_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEQ8173413.1"
                     /translation="MKISEEEARHVAKLSKLSFSESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDIAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            32067..33533
                     /gene="gatA"
                     /locus_tag="VH663_000180"
     CDS             32067..33533
                     /gene="gatA"
                     /locus_tag="VH663_000180"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEQ8173414.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFI
                     TVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIEDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            33533..34972
                     /gene="gatB"
                     /locus_tag="VH663_000181"
     CDS             33533..34972
                     /gene="gatB"
                     /locus_tag="VH663_000181"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEQ8173415.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGAIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKALSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            34969..35184
                     /locus_tag="VH663_000182"
     CDS             34969..35184
                     /locus_tag="VH663_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173416.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTAYKKTIIYAKI
                     RLTGIALEPSGVSDGYLMNHHVCPHYY"
     gene            35403..35642
                     /locus_tag="VH663_000183"
     CDS             35403..35642
                     /locus_tag="VH663_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ8173417.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIITGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            36034..36741
                     /locus_tag="VH663_000184"
     CDS             36034..36741
                     /locus_tag="VH663_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ8173418.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEVDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFLWLIDPDRDKQYIDNGSVTVVEFDDGQFTIKTIGDM
                     SYRYRGREIIEERSDEI"
     gene            36731..37474
                     /locus_tag="VH663_000185"
     CDS             36731..37474
                     /locus_tag="VH663_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEQ8173419.1"
                     /translation="MKYNSLNYLVQLLIVIVFLGGLYLFIKPEESVTPTQLNKKEIQQ
                     KDIKKTDRLRALPKVSVEDWELVLVNRDHITKEMSPELADINGISVDKRIEQATSDFL
                     AAAQAIDSQEHLISGYRSVDYQTELYQSSIKKEMANDPTLTQEAAEALVQTYSQPPGA
                     SEHHTGLAIDMSTVDTLNASDPSVAKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            37471..38040
                     /locus_tag="VH663_000186"
     CDS             37471..38040
                     /locus_tag="VH663_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ8173420.1"
                     /translation="MRKRLKFPYFLTLLACFLLLLVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            38175..39209
                     /gene="hrcA"
                     /locus_tag="VH663_000187"
     CDS             38175..39209
                     /gene="hrcA"
                     /locus_tag="VH663_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEQ8173421.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKAFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDNSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            39212..39784
                     /gene="grpE"
                     /locus_tag="VH663_000188"
     CDS             39212..39784
                     /gene="grpE"
                     /locus_tag="VH663_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEQ8173422.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            39965..41791
                     /gene="dnaK"
                     /locus_tag="VH663_000189"
     CDS             39965..41791
                     /gene="dnaK"
                     /locus_tag="VH663_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEQ8173423.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAAAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            42072..43208
                     /gene="dnaJ"
                     /locus_tag="VH663_000190"
     CDS             42072..43208
                     /gene="dnaJ"
                     /locus_tag="VH663_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEQ8173424.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYNLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            43428..44219
                     /locus_tag="VH663_000191"
     CDS             43428..44219
                     /locus_tag="VH663_000191"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEQ8173425.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGLDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            44293..44727
                     /locus_tag="VH663_000192"
     CDS             44293..44727
                     /locus_tag="VH663_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ8173426.1"
                     /translation="MEYDKINHYLVDIFNRILVIEEMSLKTSQFNDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            44728..45702
                     /locus_tag="VH663_000193"
     CDS             44728..45702
                     /locus_tag="VH663_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEQ8173427.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLANIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVRI"
     gene            45763..45987
                     /locus_tag="VH663_000194"
     CDS             45763..45987
                     /locus_tag="VH663_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ8173428.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            46189..47160
                     /gene="fabK"
                     /locus_tag="VH663_000195"
     CDS             46189..47160
                     /gene="fabK"
                     /locus_tag="VH663_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEQ8173429.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            47185..48123
                     /gene="fabD"
                     /locus_tag="VH663_000196"
     CDS             47185..48123
                     /gene="fabD"
                     /locus_tag="VH663_000196"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEQ8173430.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            48113..48847
                     /gene="fabG"
                     /locus_tag="VH663_000197"
     CDS             48113..48847
                     /gene="fabG"
                     /locus_tag="VH663_000197"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEQ8173431.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            48862..50094
                     /gene="fabF"
                     /locus_tag="VH663_000198"
     CDS             48862..50094
                     /gene="fabF"
                     /locus_tag="VH663_000198"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEQ8173432.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMIDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            50096..50596
                     /locus_tag="VH663_000199"
     CDS             50096..50596
                     /locus_tag="VH663_000199"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEQ8173433.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNPTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            50593..51015
                     /gene="fabZ"
                     /locus_tag="VH663_000200"
     CDS             50593..51015
                     /gene="fabZ"
                     /locus_tag="VH663_000200"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEQ8173434.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            51047..52411
                     /locus_tag="VH663_000201"
     CDS             51047..52411
                     /locus_tag="VH663_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEQ8173435.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAY
                     GNIIHLGERDCSLQRHNQKVLEESPSIAIGNTLRHEMGQAAVRAAEAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAINQEDITITGH
                     AIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            52420..53286
                     /locus_tag="VH663_000202"
     CDS             52420..53286
                     /locus_tag="VH663_000202"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEQ8173436.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKGQQIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            53283..54053
                     /locus_tag="VH663_000203"
     CDS             53283..54053
                     /locus_tag="VH663_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEQ8173437.1"
                     /translation="MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYQMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYKRFRKY"
     gene            complement(54276..55553)
                     /gene="serS"
                     /locus_tag="VH663_000204"
     CDS             complement(54276..55553)
                     /gene="serS"
                     /locus_tag="VH663_000204"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEQ8173438.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGETVISPK"
     gene            complement(55945..56304)
                     /locus_tag="VH663_000205"
     CDS             complement(55945..56304)
                     /locus_tag="VH663_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEQ8173439.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGMGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            complement(56418..57329)
                     /locus_tag="VH663_000206"
     CDS             complement(56418..57329)
                     /locus_tag="VH663_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ8173440.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            complement(57346..58155)
                     /locus_tag="VH663_000207"
     CDS             complement(57346..58155)
                     /locus_tag="VH663_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEQ8173441.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAAAEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGIIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            complement(58244..59236)
                     /gene="manX"
                     /locus_tag="VH663_000208"
     CDS             complement(58244..59236)
                     /gene="manX"
                     /locus_tag="VH663_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEQ8173442.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
     gene            complement(59588..60400)
                     /locus_tag="VH663_000209"
     CDS             complement(59588..60400)
                     /locus_tag="VH663_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8173443.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKTIKAVQDKGHHVIISTG
                     RPYRMALDYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTHHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
     gene            60664..62124
                     /locus_tag="VH663_000210"
     CDS             60664..62124
                     /locus_tag="VH663_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEQ8173444.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSGVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDMSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIIVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            62278..62739
                     /gene="tsaE"
                     /locus_tag="VH663_000211"
     CDS             62278..62739
                     /gene="tsaE"
                     /locus_tag="VH663_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEQ8173445.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHGSAKSRY"
     gene            62714..63238
                     /locus_tag="VH663_000212"
     CDS             62714..63238
                     /locus_tag="VH663_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8173446.1"
                     /translation="MVQQSLVIEEGVPQDAKAVLSLVTAAAKETDFITGVETILEATP
                     QELSGFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQAEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDVYHMRKSVSQ"
     gene            63247..64530
                     /locus_tag="VH663_000213"
     CDS             63247..64530
                     /locus_tag="VH663_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ8173447.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     KLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDYTASSH
                     TPTTPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(64641..64997)
                     /locus_tag="VH663_000214"
     CDS             complement(64641..64997)
                     /locus_tag="VH663_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEQ8173448.1"
                     /translation="MKVKTVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISRDLAHKWRYFNQETQAHLTEIQNRMAKYQENSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(64994..65413)
                     /locus_tag="VH663_000215"
     CDS             complement(64994..65413)
                     /locus_tag="VH663_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ8173449.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKIARAIQSATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEEDGISIQYTTHEPDFPVLEKLARQINQEVSS"
     gene            65485..66210
                     /locus_tag="VH663_000216"
     CDS             65485..66210
                     /locus_tag="VH663_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173450.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            66213..67247
                     /locus_tag="VH663_000217"
     CDS             66213..67247
                     /locus_tag="VH663_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173451.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGFAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAVLLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISIKVKERAYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNMLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            67311..68102
                     /locus_tag="VH663_000218"
     CDS             67311..68102
                     /locus_tag="VH663_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEQ8173452.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            68102..68737
                     /gene="trmB"
                     /locus_tag="VH663_000219"
     CDS             68102..68737
                     /gene="trmB"
                     /locus_tag="VH663_000219"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEQ8173453.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFFNKGQVIYRVEANF"
     gene            68802..68888
                     /locus_tag="VH663_000220"
     tRNA            68802..68888
                     /locus_tag="VH663_000220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68836..68838,aa:Ser,seq:gga)
     gene            68996..69532
                     /gene="rimP"
                     /locus_tag="VH663_000221"
     CDS             68996..69532
                     /gene="rimP"
                     /locus_tag="VH663_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEQ8173454.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            69707..70864
                     /gene="nusA"
                     /locus_tag="VH663_000222"
     CDS             69707..70864
                     /gene="nusA"
                     /locus_tag="VH663_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEQ8173455.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATVVEEPVVDDVMTAEEV"
     gene            70880..71176
                     /locus_tag="VH663_000223"
     CDS             70880..71176
                     /locus_tag="VH663_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEQ8173456.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            71169..71471
                     /locus_tag="VH663_000224"
     CDS             71169..71471
                     /locus_tag="VH663_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEQ8173457.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAVADAGFSKKMRTLME"
     gene            71491..74352
                     /gene="infB"
                     /locus_tag="VH663_000225"
     CDS             71491..74352
                     /gene="infB"
                     /locus_tag="VH663_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEQ8173458.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            74558..74908
                     /gene="rbfA"
                     /locus_tag="VH663_000226"
     CDS             74558..74908
                     /gene="rbfA"
                     /locus_tag="VH663_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEQ8173459.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRELDKKD"
     gene            complement(74961..>75954)
                     /locus_tag="VH663_000227"
     CDS             complement(74961..>75954)
                     /locus_tag="VH663_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ8173460.1"
                     /translation="TLKSTLILLGLTLAIGASTQVDASSRSWKSWFIEKYFWLKGDKG
                     YYSEQDETKFDQYVNSAGEASNKGFDLKEEQVNGTLVTKTVDNMQVHTWNENPSNKAN
                     QKVIFYLHGGSYLNNPTTYHIDMLKTLSTSLDAKIILPIYPKAPTYSYSDAMPKLVDL
                     YRDTLKETTAQNITIMGDSAGGGLALGLALALKNESLKQPKDIVLLSPWLDVTMSNPD
                     IESYESNDPILSAWGLRKVGEIWARRDPNNTKHPYVSPINGDVSGLAPITMFVGTHEI
                     FYPDVKKFDDILTAKGHQHELIVADKMNHVYPIYPIEEAKTAQYQIIDTIQKAY"
BASE COUNT        23618 a        13145 c        16089 g        23102 t
ORIGIN      
        1 ctttaccaac ctttcttgaa ctgcctgcat atttatgtta aaatgaaatg attagatgat
       61 cgaaaggaaa gagaatggcc aatattctac gcaaagttat cgaaaatgac aaaggcgaac
      121 taagaaaatt agaaaaaatt gcaaaaaaag tagaatccta cgcagaccaa atggcgtcat
      181 tgtcggatag agatctacaa ggaaaaacac ttgaatttaa agaacgctat caaaaaggtg
      241 agaccctcga acaactatta cctgaagctt tcgcagttgt tcgtgaagca gcaaaacgtg
      301 tgttgggctt gttcccttat cgtgttcaaa ttatgggagg aattgttctt cataacgggg
      361 atgtccctga aatgcgtact ggtgaaggaa aaacccttac agccaccatg ccagtttatc
      421 tcaatgctat tgctggtgag ggtgttcacg ttattactgt taatgagtat ctttcaacgc
      481 gtgacgctac cgaaatgggt gaagtgtata gctggttagg tctttcagtt gggatcaacc
      541 tagctgctaa gtctcctgct gaaaaacgtg aagcttataa ttgtgatatt acttattcaa
      601 ccaactctga agtcggtttt gattaccttc gtgacaacat ggttgttcgc caggaagaca
      661 tggtacaacg cccactcaat tttgccttgg ttgatgaagt ggactcagtc ttaattgacg
      721 aggcaaggac acctttaatt gtttctggtg ctgttagctc tgaaaccaat caattgtata
      781 ttcgtgctga catgtttgta aaaactctga cgtcagtgga ttatgttatt gatgtgccca
      841 ctaaaacgat tggattaagc gattcaggta ttgataaagc tgagagttat tttaacttga
      901 gcaatcttta cgatattgaa aatgtagcct tgactcactt tatagacaac gcccttcgtg
      961 caaactatat tatgttgtta gatatcgatt atgtggtcaa cgaagatggt gagattctta
     1021 ttgttgacca atttacaggt cgtacgatgg aagggcgtcg cttctcagac ggcttgcacc
     1081 aagctattga ggcaaaagaa ggcgttcgta ttcaggaaga atcaaaaacc agcgcctcaa
     1141 tcacctacca aaatatgttt cgtatgtaca aaaaattggc aggtatgact ggtactgcga
     1201 agaccgagga agaagaattt cgcgaagttt ataacatgcg tattattcca attccaacca
     1261 atcgtccaat tgcccgtatt gatcacactg atttgcttta cccgacctta gaatcaaagt
     1321 ttagagcagt tgtggaagac gttaaaacgc gtcatgcaaa aggacaacct atccttgtag
     1381 gtacggttgc tgttgaaact agtgatttga tttcacgcaa gctggttgaa gcagggattc
     1441 ctcatgaggt tttgaatgct aaaaatcact ttaaagaagc tcaaatcatt atgaatgctg
     1501 ggcaacgtgg tgctgttaca atcgcaacta acatggctgg tcgtggtact gatatcaagc
     1561 ttggtgaagg tgttcgtgaa cttggtgggt tatgtgtcat tggtacagag cgtcatgaaa
     1621 gccgtcgtat cgataaccag cttcgtggac gttcaggtcg tcaaggagat ccaggtgaat
     1681 cccaattcta tctttcatta gaagatgatt tgatgagacg tttcggctct gatcgtataa
     1741 aagccttttt ggatcggatg aaactagatg aagaagacac tgttattaaa tcaggtatgt
     1801 tagggcgtca agtggaatct gctcaaaaac gggttgaagg aaataactac gatacccgta
     1861 aacaggtctt acaatacgat gatgtgatgc gtgaacagcg tgaaattatc tatgctaacc
     1921 gtcgtgatgt cataactgct aatcgagatc ttggcccaga aattaaagct atgatcaagc
     1981 gtactatcga ccgtgctgta gatgctcatg cacgtagcaa tcgtaaggat gctgtggatg
     2041 ccattgtgac ctttgcacgc acgtcacttg ttccagaaga gtctatcagt gctaaagagt
     2101 tacgtggttt aaaagacgaa caaatcaaag agaagcttta tcaaagagcg cttgctatct
     2161 acgaccagca actatcaaaa cttcgtgatc aagaagctat tattgaattc caaaaagtac
     2221 tcattttgat gattgtggat aacaaatgga ctgaacacat tgatgcctta gatcaacttc
     2281 gtaatgctgt tggacttcga gggtatgctc aaaataaccc tgtggtggaa taccaagcag
     2341 aaggtttcaa gatgttccaa gatatgattg gtgctatcga gtttgatgta acacgtacca
     2401 tgatgaaagc acaaattcac gaacaagaac gtgaacgtgc aagtcaacgt gccacaacag
     2461 cagcacctca aaacatccaa tcgcagcagt cagctaacac cgatgattta cccaaagtgg
     2521 agcgtaacga agcttgtcca tgtggttcag gcaaaaaatt caaaaattgt cacggtcgta
     2581 aatccttctc ataagaacga gacacaacag gacaaagtta ggtatataga aagaagctga
     2641 gaataattct cagcttctca ctgtttaaat cttctaacat taagatgaaa taaggtttgg
     2701 tggaaagtaa tgattgttgg acatggaata gacttacaag agatttcagc tattgaaaaa
     2761 gtttatcaaa gaaatcctcg ctttgctcaa aaaattttaa ctgagcagga attggctatt
     2821 tttgagagtt tcccttacaa acgtcgactc agctatttgg caggacgctg gtcaggtaag
     2881 gaagcttttg ctaaggctat agggacaggt attgggcggt taacctttca agatattgag
     2941 attttaaatg atgttagagg atgtcccatt ctgacaaaat ctccgtttaa aggaaatagt
     3001 tttattagca tctcacatag tggcaattat gtacaggcta gtgttatttt ggaggataaa
     3061 aaatgatttc aagtttccat cgcccaacag ttgcaagggt caatttacaa gctattaagg
     3121 agaatgttgc cagtgttcaa aagcatattc cactaggggt aaaaacgtat gcagttgtca
     3181 aggctgatgc ttatggtcat ggtgctgtcc aggtgtcaaa agcactccta cctcaagtgg
     3241 atgggtactg tgtgtcaaat cttgatgagg ctttgcaatt acgtcaagca ggtattgata
     3301 aagagatttt aattcttgga gtcctcctgc caaatgaatt agagttagca gtcgctaatg
     3361 ctattactgt tacaatcgct agtttagact ggatagcttt agctagactg gagaaaaaag
     3421 aatgtcaagg cttaaaagtt catgtaaaag ttgattctgg tatgggacgg atcgggcttc
     3481 gttcttcaaa agaagtcaat ttattgattg atagtctaaa agagttgggt gctgatgtag
     3541 aaggtatttt cactcatttt gccacagctg atgaggcaga tgatactaaa tttaaccagc
     3601 agttacagtt ttttaaaaag ctgatagctg gacttgagga taagcctcgt ttagtacatg
     3661 ctagtaattc agccacaagt atctggcata gtgataccat ttttaatgct gttcgtttag
     3721 gaattgtcag ttatggtttg aatccaagtg gttctgatct aagcttaccg tttccactgc
     3781 aagaggcttt atctctagaa tctagcttag tgcatgtcaa gatgatttca gctggtgata
     3841 cagtcggtta tggagctact tatactgcca aaaagtctga atatgtaggg accgtcccaa
     3901 tcggttatgc agatggctgg accaggaaca tgcaaggctt ttcggtgtta gttgatggac
     3961 aattctgtga aattataggg cgtgtatcga tggatcaact gaccatacga cttcccaaag
     4021 catatccttt aggaacaaaa gtcactttga ttggcagcaa tcagcaaaaa aatatttcta
     4081 caacagatat cgcaaattac cgtaatacaa tcaattatga agttctatgc cttttaagtg
     4141 accgtattcc tcggatatat taagaaaatc atgtaaaaat aattgcattg tgcttatctt
     4201 taatgttata ataaagtgag gacattagaa agaagtgact aattaatata tggataaaaa
     4261 caaactatta agagttgcca tgctactaag tctcttagcc ccgacagcag aaagcatgac
     4321 agtgttggct caagatgtaa tgcttgagac gcataaagca actacaaatg aaaccagtga
     4381 ttcttcttca aaagaggaaa ataataaaaa tgcagcacct acaacatcag ataaaactga
     4441 ccaaggtccc cttgatgctt ctgcagaaac aaactctaat agtcttgtta acgcggatga
     4501 taaaaaaaga agcgattcta gtcagtctgc tgtaggttct tcggacaaca aggcagaagc
     4561 agaaaaccag gtagatgata aatcaactga tcattcgaaa tcaactgatc attcgaaacc
     4621 gactgaccag ccaaaaccga ctgatcagcc caaaccatca ccatctaaag ttgatacggc
     4681 acctgcttct tcattgtcga aacaactgcc agaggcaaga actcccattc agtcgttgtc
     4741 accttacgta tcagatttag atttgagtga gatagatatc ccttctgtca acacatacgc
     4801 ggcatatgta gagcattgga gtggtaaaaa tgcctatacc caccatcttt tatctcgccg
     4861 ttatggtatt aaagctgacc agattgatag ttacttaaaa tcaacaggca ttgcctatga
     4921 cagcacacgt attaatggtg agaagctatt gcaatgggaa aagaaaagtg ggctggatgt
     4981 tcgagctatc gtagctattg cgatgtctga gagttcttta ggaactcaag ggattgcaac
     5041 tttgcttgga gctaatatgt ttggctatgc agcttttgat ctagatccga ctcaagcaag
     5101 taagtttaat gatgatagtg ctattgtcaa aatgacacaa gacaccatta ttaaaaacaa
     5161 aaatagcaat tttgcacttc aagatttaaa agcggctaag ttttcacgag gtcaattaaa
     5221 ctttgcaagt gacgggggtg tttattttac tgatactact ggtagtggta aacgtcgcgc
     5281 acaaattatg gaagacctgg ataagtggat tgatgaccat ggtggcacac cagctattcc
     5341 agccgaattg aaagtgcagt catcagctag ttttgcatct gtgccagcag gttataagct
     5401 ctctaagagt tatgatgtct tgggttatca agcttcgagt tatgcttggg gacaatgcac
     5461 ttggtatgtg tataatcgcg ccaaagaatt gggttaccaa tttgatcctt ttatgggaaa
     5521 tggtggagat tggaagtata aagtagggta tgccctttca aagactccaa aagtaggtta
     5581 tgctatttca tttgcaccag ggcaagcggg cgctgatggc acttatggcc acgtatcaat
     5641 tgtagaagat gttagaaaag atgggtctat tcttatttca gagtctaact gtatcggctt
     5701 aggcaagatt tcttatcgta cctttacagc tcagcaggct gaacagctaa catatgttat
     5761 tggcaagagt aaaaactaat aactaatgac ttttcttaag gcttaatttt cttagcggga
     5821 gttaggcctt ttaaactgtt ggtgtaacac gataaagttt caagtaaata aatgattttg
     5881 agacaatagc acgattaggg ctattgtctt ttttttattg tgatgaatat gcagtagtga
     5941 catctcatgt tgtctcctaa cacaattatg gagcttataa cgtgttacat gttttgtgaa
     6001 tgaagtaatg attgaatcag ccttagtaat aactatccta aggctagctt tatctcagta
     6061 atagtgttgt agaggaattc ttttatcaga gatatcctca ttatcaatcc aggcttatta
     6121 ctagtgtctt agctcacata gtggagctat ttaacagaca attggaccat tggaaacaat
     6181 cttgacaaaa taatcacaaa gtgatagatt aagttcagtt aataaaaaag ttagcttatt
     6241 cataaaaatt tttagaaagt tgattgaaaa tgaaaaaaat cagcaaatgt gcgtttgtgg
     6301 caatatctgc ccttgttctc attcaggcta ctcaaactgt aaaatcacaa gagcctttag
     6361 ttcagtcaca actcgtgaca acagtagctt taactcagga taatcgactt ttagttgaag
     6421 agataggccc ttacgctagt caatcagctg gaaaagagta ttataaacat attgaaaagg
     6481 ttattgttga taatgatgtc tatgaaaaaa gcctggaagg cgagcgaacc tttgatatta
     6541 actaccaagg gattaagatc aatgctaacc ttattaaaga cggtaagcat gaattgacta
     6601 ttgttaataa aaaagatggt gatatcctaa ttacctttat taaaaagggc gataaagtga
     6661 cgtttatttc agctcaaaaa ttaggaacaa cagatcatca ggattcatta aaaaaagatg
     6721 tgctcagtga taaaccagtg ccacaaaacc aaggcacaca aaaagttgtt aaatctggga
     6781 aaaatactgc taacttgtca ttaataacaa aattgagtca agaagatggt gcaattttat
     6841 ttccagaaat tgatcgttat tctgataaca aacagataaa agcattgact cagcaaatca
     6901 ccaaggttac agtcaatggt acagtttata aagatcttat ttcagattct gtaaaagata
     6961 ctaatggctg ggtctcgaat atgacagggc ttcatcttgg aacaaaagct ttcaaagatg
     7021 gagaaaatac aatcgtgata tcctcaaaag gatttgaaga cattaccatt accgttacca
     7081 agaaagatgg tcaaatccat tttgtatctg ccaaacaaaa acaacatgtg actgctgaag
     7141 acagacaatc aacaaagttg gatgtcacca ctttggaaaa agctatcaaa gaagcggatg
     7201 cgattattgc taaagaaagc aacaaagacg cggtcaaaga tctggctgag aaacttcaag
     7261 tcatcaagga ttcttataaa gaaatcaaag atagtaagct actcgccgat actcatcgac
     7321 tgttaaaaga taccatcgag tcttatcaag caggtgaggt ttctattaac aatcttacag
     7381 aaggaaccta tacgctaaac tttaaagcta ataaagaaaa ctcagaagag tcctccatgc
     7441 ttcaaggtgc ttttgataaa agagccaaat tagtggttaa agcagatggt acaatggaaa
     7501 tttccatgct taatactgct ttgggacaat ttttgattga cttttctatt gaaagcaaag
     7561 ggacctaccc agcagcagtg cgtaaacaag ttggccaaaa agatatcaat ggtagctata
     7621 ttcgaagcga atttaccatg cctattgatg atttggataa attacacaaa ggtgctgttt
     7681 tggtatcagc catgggaggt caagaaagtg atttaaacca ctatgacaaa tacaccaaac
     7741 ttgacatgac ctttagtaag accgttacca aaggctggag tggttatcag gtagaaactg
     7801 atgataaaga aaaaggggtt gggactgaac gtcttgaaaa agttttagtt aaacttggca
     7861 aagatttaga cggcgatggt aaattatcaa aaacagaatt agaacagatt cgaggtgagt
     7921 tgcgtctaga ccattacgag ttaactgata tttctttatt gaaacatgct aaaaatatta
     7981 cagaactaca tctggatgga aaccaaatta cggaaattcc aaaagagtta tttagtcaaa
     8041 tgaagcaact tcgatttctt aacttaagaa gtaatcattt aacttatcta gacaaagata
     8101 catttaaaag caatgctcaa ttaagagaac tctacttatc aagtaacttt attcactctc
     8161 ttgaaggagg actattccag tcgcttcatc acctggagca acttgatctt tccaagaatc
     8221 gtattggccg actttgtgat aacccatttg aaggattgtc tcgtctgact tcattaggtt
     8281 tcgcagaaaa tagtcttgag gagatacctg aaaaagcgct agagcctcta acatcactta
     8341 attttatcga cttatctcaa aataatctag cgctactgcc aaaaacaata gaaaaattgc
     8401 gcgccttaag cactattgtg gcaagtagaa atcatattac tcgtattgat aatatttcat
     8461 ttaaaaatct tcctaaatta tctgtactcg atttatcaac taatgaaatt tcaaatcttc
     8521 caaatggtat atttaaacag aataaccaat taacaaaact tgattttttc aataacttgc
     8581 ttactcaggt tgaagaatca gtatttccag atgttgaaac gcttaattta gatgtgaagt
     8641 tcaatcagat aaaaagtgtg agtccaaaag taagagctct tatcggacaa cacaaactga
     8701 ctccacaaaa acatattgca aaacttgaag cttccttaga tggcgaaaaa ataaaatatc
     8761 atcaagcttt cagtctttta gatttgtatt attgggagca aaaaacaaat tctgccattg
     8821 ataaagaact agtgtctatt gaagaatatc aacaattgtt acaagaaaaa ggttcagata
     8881 ctgtttcttt acttaatgat atgcaagtcg attggagtat tgtgattcag ttgcaaaaaa
     8941 aagcttccaa tggacagtat gtgacggttg acgaaaagct tctctcaaat gatccgaaag
     9001 atgacttaac gggagagttt tctttaaaag atccaggtac atatcggatt cgcaaagctt
     9061 taataacaaa gaaatttgct actcaaaaag aacatatcta tttgacatct aatgatatcc
     9121 ttgtggcgaa aggaccacat tcacatcaga aagatttagt tgagaacggc cttagagcat
     9181 taaatcaaaa acaattgcgt gatggtattt actatttaaa tgccagcatg ttaaaaactg
     9241 acttagcatc tgagtccatg tcaaacaagg ctattaatca tcgggtgact ttggtagtta
     9301 aaaaaggtgt tccctattta gaggttgagt ttagagggat aaaggttggt aaaatgttag
     9361 gctaccttgg tgaattgagc tatttcgtag atggttacca aagagattta gctggtaaac
     9421 cagttggtcg aacaaaaaag gcagaggttg tgtcttattt cacggatgta actggcctac
     9481 cattggcaga tcgttatgga aaaaactatc caaaagttct gcgtatgaaa ttgattgaac
     9541 aagcgaagaa agacggactt gtgccattac aggtctttgt gcctatcatg gatgccattt
     9601 caaaagggtc tggccttcaa accgttttta tgcgtttaga ctgggcaagc cttacaacag
     9661 agaaggcaaa ggttgtcaaa gaaactaata atccacaaga aaatagccat ctaacttcaa
     9721 cagatcagtt gaaaggacct caaaatcgtc aacaagaaaa aacacctaca agtcctcctt
     9781 cagcagctac tggtattgct aacttaactg atctcctggc taaaaaagta accggccaat
     9841 caactcaaga aacttctaag acagatgata ctgataaggc agagaaattg aagcagttag
     9901 tgcgtgacca tcaaacatca attgaaggta aaacggcaaa agatactaag actaaaaaat
     9961 ctgataagaa acatcgttcc aatcaacaat caaatggtga agaaagtagc tctcgttatc
    10021 acttaattgc aggtctatct agctttatga tcgtagctct gggattcatt attggtcgaa
    10081 agacattatt taaataagaa acttagtacc aagtgaaata acaaaaaagc tagtcagcaa
    10141 gagctaaaaa catacctatt tccgacagga ttcctgatac aggttgaagt ggtcgcaaga
    10201 taggtgtgtt tttcacattt ggagatgtta taaaatggga atcatttaaa aaatgaaata
    10261 agatgtttaa gaaaagatag acaagaggag catatgacaa aggtagtaat aaagcaattg
    10321 ttgcaggtaa tagtagtgtt catgataagt ttatcaacaa tgacaaatct tgtgtatgca
    10381 gataaaggtc aaatttatgg atgtattatt caaagaaatt atcgtcaccc gatatctggg
    10441 cagatagaag atagtggagg ggagcattcc tttgatattg gtcaaggtat ggttgaaggg
    10501 actgtctata gtgatgccat gctagaagta tctgatgctg gaaagatcgt tcttactttt
    10561 cgtatgagct tagctgatta ttcgggtaat tatcagttct ggatacaacc tggaggaact
    10621 ggaagctttc aggcagttga ttacaatatc acccaaaaag gtactgatac caatgggaca
    10681 acactagata ttgctattag tttgcctact gtaaatagta tcattcgagg aagcatgttt
    10741 gttgaaccta tgggcagaga agtggtcttt tacctgtcag cttcagagtt aatacaaaag
    10801 tattcaggta atatgttggc ccagcttgtt actgaaacag acaattctca aaatcaagaa
    10861 gtaaaagaca gtcaaaagcc tgttgataca aaactaggag aaagtcaaga tgagcctcac
    10921 acaggtgcta tgataactca gaataagcct aaagccaact catcaaataa caagagtcta
    10981 tcagataaaa aaattcttcc ttctaagatg ggcttaacga cttctttaga gttaaaaaaa
    11041 gaagataagt ttcggtctaa aaaagactta tcaatcatga tctactactt tccaactttt
    11101 tttctgatgt taggagggtt tgctgtatgg gtttggaaaa agaggaaaaa aaatgataaa
    11161 acgatgtaaa ggaattggtc tagtcttaat ggccttcttt ttggtagctt gtgtgaatca
    11221 gcaccctaaa acggctaaag agactgaaca gcagagaatt gtagccactt cggttgctgt
    11281 ggttgatatc tgtgaccgtt taaatttaga cctcgttggg gtttgtgata gtaaattata
    11341 tacccttcct aaacgctatg atgctgttaa gcgtgtgggt ttacccatga atcctgatat
    11401 agagttgatt gcttctttga aaccaacttg gattttgagt cccaattctt tacaagaaga
    11461 tttggaaccc aagtatcaaa aattggatac tgagtatggt tttttgaact tacgaagtgt
    11521 tgagggcatg taccagtcta ttgatgattt agggaacctt ttccaacgtc aacaagaagc
    11581 aaaagaattg cgccagcaat accaggacta ttatcgtgct ttccaagcta aacgtaaggg
    11641 gaagaaaaag cctaaagtgc ttattcttat gggcttgcca ggtagttatt tggtggcgac
    11701 gaaccaatct tatgtaggga atcttttgga cttggcaggt ggtgagaatg tttatcagtc
    11761 agatgagaaa gaatttctat cagttaatcc tgaagacatg ctagctaagg agcctgactt
    11821 gattttacga acagctcacg ccattccaga caaggtaaaa gtgatgtttg acaaagaatt
    11881 tgctgaaaat gatatttgga aacattttac ggcagtcaag gaagggaaag tctatgattt
    11941 ggacaatacc ctgtttggca tgagtgctaa attgaactac ccagaagcct tggacacctt
    12001 aacacagctt tttgaccacg tgggagatca tccgtaacat gacagcatca gatttacaaa
    12061 gggatcaatc cctattggct aaaaaacgtt tcagaagtgg tctttattta gttcttttaa
    12121 ttctcagcct cctatttttg gcggttattg ccttatccct tggtggtttg gcggtttctt
    12181 atgaggcaat tgtcaaggga ctgtttgtgg cttatgaccc ccaagtggct ttgatttatg
    12241 atttgcgctt tccaagaatt gtcattgctt tattagcagg agcagggata gctgtttcgg
    12301 gagttctttt tcaagcagtg ttgaaaaatc ctatttcaga ccctgctatt atagggattt
    12361 gtagtggtgc tagttttatg gttttagtct ctagtttact tctaccgcag ttgctgctgt
    12421 atggtccaat cgtatccttt ttaggaggcg gagtgtcttt cctgctgatt tatgctttag
    12481 catggaagaa gggtcttaat cctattcggc ttattctaac agggattgct attaatgctt
    12541 tatttatggg gttatcaact gctttaacca gctttttcac ctcagctagt cccatggtca
    12601 atgctctttt agcaggtcat attagtcaaa aaacatgggc tgatgtaggg gttttattcc
    12661 cttatacatt cattggcttg ttactagctt tgttattgtc aaaaacttgt aaccttcttc
    12721 ttttagatga tcaggtgatt cgtcatttag gaatcgatgc tacagcgtta cggttgggaa
    12781 tttccttggt tgctgtttta ttggcttcgg tagccacttc gatcgttgga gtggtttctt
    12841 tcctaggctt gattgtccct catatgagcc gcttgctggt tggaagtaag caccaaatct
    12901 taattccttt ttcagctttg cttggagcct ttgtcttttt gctagccgat actttaggaa
    12961 gaagtcttgc ttacccattg gaaattagcc ctgcgattat aatgagtatt gtaggcgggc
    13021 cttactttat ttacctgcta aggaggtctg atattatatg agtaagaaca gtagtcatat
    13081 tcaggtgcaa aacctttcct ttgcttatga ggaaaaactt gttttagatg acctgacctt
    13141 tagcattcca aaagggaaaa ttaccactat tatgggggca aatggcagtg gcaagtcaac
    13201 cttattacaa ttattaacca aaaaccttcc gttaaaacaa gggcagattt ggcttgagca
    13261 ggaagaatta gcaacgattt ctatcaaggc ttttgcgaaa aaagtggcag tggtacacca
    13321 ataccatcaa gtggtcgaag gtttgttagt gggtgaattg gtagccatgg ctaggcttgc
    13381 ccgtcgttct ttttttaaaa ctactaccaa agaagatgag gaacgccttg cttgggcctt
    13441 agaagtaacg gctttgagtg attatgttta tcgtgacatc caacaccttt ctggtggtga
    13501 acagcagagg gtttggattg ccatggccct tgcccaggat acagatatta tttttttaga
    13561 tgagccaacg acttatttgg atattaagta ccagcttgac attctcaagc tgattactaa
    13621 aatcaaccaa gagctaggcc taacgattgt gttagtatta catgacatca atcaggcctt
    13681 gtctctttca gaccatctta ttggactgaa agatgggcaa ctttatgccc aagggcaacc
    13741 aagagacctc cttgatcaac agtttatcag agatgtcttt ggtgttgaat tgccctttgt
    13801 tgagagaaat ggtcaacagt ttgtcctcac ttcattggtc aatgaaacca atggtattag
    13861 aactcaaagg agagatagga acaagaacag atgatggaaa cagaagatac aatatcaagg
    13921 ggaaaaagga aaagactgtt aaaacgatta cgtgaacgaa ttgcaccaaa aaggtatttg
    13981 ctttatgtgt cagccttttt atcttggtta caatttgtga tgaggatgat cagtttttac
    14041 ctcattgcca aaacgttttc gacctttatt ttgggtcatg ctattgcctt aggacgcttg
    14101 gcaggtttac tcctactttt aaatgtggta ggctttgttc ttgctatatt aggcaagcaa
    14161 ctgcaaggga tcgcttcaca atttgctaga gattcactca aacaatcttt ctttgaggct
    14221 tttattgatt tagacgggca atttgatgct catgccagtg atgctgatat tttaacgttg
    14281 gcttcacagg ggattgatag tttggatact tattatggtt actatttgtc tttgtctatg
    14341 agaaccaagt ggaactgcac aactattatg atactagtct ttttaattta tccattggca
    14401 ggccttgtct ttttaggagt tctgcctctg attccacttt caattgttgc tatgcaaaag
    14461 cggtctaagc aaattatgag tcattattgg tcttcttata tggatgttgg taatttattt
    14521 atggatgact taaaaggtct taatacactg tattcttatc aagcaacaga acgttacgag
    14581 caagaatttt cgggtaaggc agaacagttt cgaaaggcta ccatgtcttt gcttggcttt
    14641 cagttacaag cagttggtta tatggatgca gttatgtatc tgggaattgg tttgtcaggt
    14701 tttttagccg ttcaagcttt ggcaacaggc cagttatctt tttttaattt cctctttttt
    14761 ctcttgattg ctacagaatt ttttacaccg attcgtgagc agggttatgg catgcacttg
    14821 gtcatgatga ataccaaaat ggctgaccgt atctttagtt tcttggatag tgttccagca
    14881 aggaaagata acaagagtaa aacagcaata aactttaacc agattgacat ccaaaatatt
    14941 tctttagctt atgagaaaaa aacagtgctt agtggggtta ccatgacatt aacgaagggc
    15001 caactaacag ctatcgcggg ggtgtcagga caaggaaaaa caagtctagc tcaactcctc
    15061 ttgaaacgcc agtctgcaac gactgggcat attttatttg acggtttaga cagtgacaat
    15121 ttgtcgcaag aaactatcaa ccagcaggtt ctttatgtat cggaccaatc taccttattg
    15181 aaccgtagca tttacgataa tttaaggtta gcagcaaatc tctctaaaaa agaaatctta
    15241 gattggatag atcaacatgg tttgttaagc ttcatcaact ggctaccaga tggtcttgac
    15301 acaatagttg gtgaaaatgg taacctgtta tcaccaggcc aaaagcaaca ggtgatttgc
    15361 gcaagagctc tattaagtaa gaggtctctt tatatctttg atgaggcaac atctagtcta
    15421 gatgcagaaa atgaacgtat tattgacaat ttaataacga gattagctaa gacagcaata
    15481 gttattgtga ttactcataa gatgtctcga ctgaaaggag ctaaccaagt actcttttta
    15541 aacacagggc agcctgcttg tttaggcaaa ccttgcgacc tttatcggga ccaaccaact
    15601 tatcgtcacc tagttgatac tcaagcaaga ctggaggcaa gtatttatgg ctaaacctaa
    15661 cagatctacc ttaagcctgg tttggcgttt attaacccta atgaaaactc ttctaccttg
    15721 gattatcttg gctgttggct ttgctgttat gggttttgtc atcacggtaa gtattccaac
    15781 tggaatagct tatttaggac tactagctat tagacaagaa gtgattccaa tattagcttt
    15841 atatcttttg attgctctag cctttttaag aggttttgtt cgttatggag agcattattt
    15901 tggtcatttt gttgcttttc atagtttagc agcttttagg aacttgatct ttaagaaact
    15961 aagagctctt tctccagctt gcttggatag tcaagacagt ggttatttgc ttaaaatgat
    16021 tggagaagat attgaggcat tagaagtttt ttttgcccac acaatcgcac ctatttgtac
    16081 agcaatattg tctgcaggtt taatgttctg gtacttttgt caaagtagtt ggcagttagc
    16141 cttattagct ttggcaacct atgcttgttt agccattgtt atacccattt attttgctaa
    16201 tattttacag gttttactaa aaagccaaaa tgaaggtcga aaagattacc tgtcttattt
    16261 tttagagagt ttacgatcag tcaaagatct tttgcaattt caggtattag acgagcaatt
    16321 tgaacgatta ataaaaaaaa gtaatcatgt aaatgctctt gatcgtaacg tagcgcaagc
    16381 ccaatttttg caaatggcat tgactttttt ctggcttggc ttaatgatct tagcattttc
    16441 ttatatggtt tttgatggga tataccatga cagcttatca tttgataaag gcttattaac
    16501 ttttattgct ttcactgctt cgttttctcc ttttttagaa ctaggacgtt tgccattagg
    16561 ttttaagcgt gctatgaatg cggctcgaaa tatttttgac cttctagacg aaaatgtcat
    16621 agtagacgaa ggcacaaaac agatagatcg tttaaggagt gtttcttttg aggacctcag
    16681 ttttgcttat ccaaagcgcc aagagctggt ttttaaggat ttatcagtta cctttcaaga
    16741 aaaggggatt attggcatta aaggcgaatc aggatctggt aaatcaactt tggtgaaatt
    16801 aattatgaaa tggtataatt ggaagacagg agatattttt cttaatgata ggaatagttg
    16861 cttactaaat gctgctaaat tacaagatac tattgcttat gtgccacaaa cagcgcagct
    16921 tttccaacag tctatccgtg aaaatcttac ctttggccgt caagatattt cagatgagtc
    16981 catttggaat ttagctgagg cttgtggtat gaaagacaga ctgttagctt gtaaggaagg
    17041 tttggatacg attatcaaga gtccttctga tttttcagct ggagaaggtc aacgtttaga
    17101 gttgatgcga gctttattga aagatgcgag ttgctatatt tttgatgaac ccacgtcaaa
    17161 tttagattct ctatatgaag ctatcttgct tgatttaatt aaaacacatt gtcaaggatt
    17221 ggtcttctta atttctcaca ggccatcaac cctagcttgt gctgatcatc tattttgcgt
    17281 aaaaaatggt ttcctaaaag aggtgaataa aaaatgaaac agttaacaat aaaagatatt
    17341 atgataacag gcgcatttgc cgcgctctac tttatttgtg ttggtctagg cacgtttatt
    17401 aacatctttt ttgatcgctc aggaaatatg ttatatgccc ctgcctttgc agctttactg
    17461 gggggaacaa tttattttct tttgattgct aaattgcaaa catttgggcc aataacatta
    17521 ttaggattga tgatgggagg ttttttcttc cttagtggtc acttttttgc cgcgatgttt
    17581 cccagcatta ttttcggttt aatagcggat attattgctt ctttaggaaa atacaagtca
    17641 aggtttttta atgacctatc ttatatggtt tttgcttttg tcaatagtgg ccctattttg
    17701 ctgatgtggt tgttgaaagc tgcctatgtt gaaagtttgt tagcccgcgg taaaagtatg
    17761 acctatgtca atcgcattat gtatgatttt agtttagggc atgttatttg gtttgtagga
    17821 attgttcttg caggagcctt attagggtca gtttttgcac attatttact aaagaaacat
    17881 tttatcaaat caggattgtt atcatgaggc ttgatgttcg caccaaactg cttttgttgg
    17941 tacttgccaa tgcctgcttc ttttttcgtg ttgatggctt tttagaattt ataattgtta
    18001 tttttctctt attacttttg tctgctttaa ataagaaaaa gctagcgttc aagttggcgg
    18061 ttgtttatct gttaatgatc ggtctatctg taatcccgct atcgattttc ccttcttatc
    18121 ttgaccactt attgagtttt gtctcaatag caggaaggtt agttttccct tctttactag
    18181 caggtttaat tactattaag acaactacca tctacgaatt ggttcatgga ttacgcaaat
    18241 ggcatttccc agaagtctgg ctcttgacct tagccgttat gtgccgtttc ataccaatga
    18301 ttagacaaga atgttgtgtt attcatcgct ctttaacaat cagggggatt attttaacaa
    18361 aatggtctat tttgatcaga cctaaacaat acttggagta tttaatggtt ccattgcttt
    18421 tatctttgat aagaagtagc caagaactaa ctatcgctag tctaactaaa gggctagcag
    18481 tcaacaaagg caccagtgaa tgctttagct cacacttaac atggaaagat tggggggtgc
    18541 agatttggat tactgttatc ataatcatca cgatacttca atgatttcag cagaacaact
    18601 tgtttttacc tatcatgatc aaaaaaatcc agcttgtcag gtaccgactt gtcaaattgc
    18661 ctctggacaa ttcattgttt tgtgtggacc ttctggttca ggtaaatcta cctttttaaa
    18721 acttcttaat ggcataattc ctgattatta tgctggaaaa tatgaaggta gactagatgt
    18781 ggctgattgc caagcaggca gagacagtgt tgaaacattt tctagatctg tggcctctgt
    18841 ttttcaaaat ccagccagtc agtttttcta tcgcgaagtc caacacgaac tggtctttcc
    18901 ctgtgaaaat caaggtctag atgcaaaggt tatcatgaag cgtctttgga ctttagcaga
    18961 agattttgct tttgcagagc tccttaacaa ggatatgttt ggattgtcag gagggcaaaa
    19021 acaaagagtt gctattgcaa cggccattat gcaaggtact aatattatgc tttttgatga
    19081 gccaactgct aacttagata gtgcaggcat agctgctgtt aaagcctatc ttacccaatt
    19141 aaaagcagca ggaaaaacca ttattgtagc cgagcatcgc ttacattact tgatggattt
    19201 ggcagataac tttttctact ttaaaaatgg tagattaaca gataagttaa caacacaaaa
    19261 tttgctagcc ttgacagatg aacaacagca agatatggga ctacgtcgct tagacttgtc
    19321 tgacctaaaa cctgtgttag ctggtaagat tgagagtcaa cattatcgcc cagatgatag
    19381 cctgtgtatt gagcatctaa cagttagagc gggaagcaaa atacttagat gtattgaaca
    19441 gcttagtttt gctgcaggaa gtatttctgg cattacagga tcaaacgggc ttggaaaatc
    19501 gcaattagtt tattatatag ctggtatctt agacgataaa aaagcaacta ttaagtttca
    19561 aggcatacct ttatctgcca agcaacgttt atcaaagact agtattgtct tacaagaagt
    19621 ttctttgcaa ctatttgcag aatcggtttc aaaggaagtc aacttgggcc atgaacgaca
    19681 tccacggacg acagaagtaa tagagcggtt atctttaaca actcttttag aacgtcatcc
    19741 agctagtttg tcaggaggtg agcaacaaag agtgatgatt gccgctagtc ttttggctga
    19801 taaagatatc ttaatttttg atgagccaag tagcggactt gatttgttac aaatgaaggc
    19861 tttagctaat cttctcatgc agctgaagac ccaacataag gttgtcatcc tcatttcaca
    19921 tgatgaagaa cttctaagta gtgtctgcga taccatttat caaatgctac cttaacgagc
    19981 ccctagaact gtttaaaagt actaaaaggc ttttatctga aaataaatag gaactcttgt
    20041 ataatagata caagagtcag aacgtagaca accccagttt tatgctcaaa aagcatgaat
    20101 agctctgctg atttgacatt cggtttccga tttttacaca caaagtgagc caaaacagat
    20161 gctttttact gtggtaaaag agtggcgtca atacgagatt gacgccactc gggatttttg
    20221 agacctcagg ctcaaaaata aaggcatgga attccaaaga acgtcgctgc cgtaagcacc
    20281 acctaaggaa agtatcacga caaactttgt cttcaatctg acaagagttt ttgtgaaggg
    20341 aaaaagataa tgatattaac agctcctatg tccaacttaa agggatttgg accaaaatca
    20401 gcagaaaaat ttcagaaatt agatatttat acagtagaag atttactgct ttattatccg
    20461 tttcgctatg aagattttaa atcaaaatct gtttttgatt tagtggatgg tgaaaaagca
    20521 gtcattacag gcttagtcgt tactccagct aatgtacaat attatggttt taaacgtaac
    20581 cgtttaagtt tcaaattgcg tcaaggggaa gctgtcttaa atgttagttt ttttaatcaa
    20641 ccctatttag ctgataaaat agaacttggt caagaggtag ctgtttttgg taaatgggat
    20701 gccactaaat cagctattac tgggatgaaa gttttagctc aagttgaaga tgacatgcaa
    20761 cctgtttatc gcgtagctca gggaatttca cagtctactt tgattaaagc tattaagtca
    20821 gcttttgaaa tcgatgcgca tttggaattg aaggaaaatt taccagatac tttattggaa
    20881 aaataccgat tgatgggtcg tagtcaggct tgtttagcta tgcatttccc aaaagatatc
    20941 acagagtata agcaagcgct ccgtcggatt aaatttgaag aattatttta ctttcaaatg
    21001 aaccttcaag ttttgaaagc cgaaaataaa tctgaaacaa atggtttgcc tattctttat
    21061 agtaaacatg ctatggagac aaagatttcc tctttacctt ttattctaac gaatgctcaa
    21121 aagcgctctt tagatgacat attatctgat atgtcatcgg gagctcatat gaatcgttta
    21181 ttgcaaggag atgtaggatc aggaaagaca gtcattgctg gtctatcaat gtatgcagct
    21241 tatacagcag gttttcaatc ggctttgatg gttccaacgg aaatcctagc tgaacaacac
    21301 tacattagtc tgcaagagtt atttccagat ctatcaattg ctatattaac ttcgggtatg
    21361 aaagccgctc tcaagcgtac ggttttagca gctattgcaa atggctcggt tgatatgatt
    21421 gtaggaactc atgctcttat ccaagactcg gtacagtacc ataaactggg gcttgtcatt
    21481 acagacgagc aacatcgttt tggtgttaaa cagcgtagaa ttttccgtga aaagggagaa
    21541 aatcctgatg ttttaatgat gacagccacc ccaattcccc gaactctagc aatcacagct
    21601 tttggagaaa tggatgtttc tgttattgat gaattacctg ccggtcgtaa acctattatg
    21661 acacgctggg tgaaacacga gcagctaggg actgtgttgg aatgggtaaa aggtgaattg
    21721 caaaaagatg ctcaagtgta tgtcatttca ccgttgattg aagaatcaga agctttagat
    21781 ttaaagaatg cagtagcatt gcatgctgaa ttatctactt attttgaagg aattgctaag
    21841 gttgctcttg tacatggacg tatgaaaaat gatgaaaaag atgccataat gcaagatttc
    21901 aaggataaaa aaagtcatat tttagtatcc acaacagtta ttgaagtagg ggtaaatgtc
    21961 ccaaatgcaa cgatcatgat tattatggat gccgatcgtt ttggactaag tcagttacat
    22021 caacttcgtg ggcgtgttgg tcgtggacat aaacaatcat acgctgtttt agtggctaat
    22081 cccaaaacgg attcggggaa aaaacgaatg acaatcatga cagaaacgac agatggtttc
    22141 gttttggctg agtcggattt aaaaatgcgt ggttctggtg aaatctttgg tactcgtcag
    22201 tctggaattc cagaatttca agtagctgat atcgttgagg attatcctat tttagaagaa
    22261 gcacgcaaag tttctgcagc gattgtttct gatcctaact ggatatatga aaaacagtgg
    22321 cagttagtag caaaaaatat tagaaaaaaa gaaatttatg attaacttaa accaagtcgt
    22381 tgttttacaa atatttagtt agttttgaga taaactgtta taaagtataa agaggtcact
    22441 gtttctggcc ttttttactt atgctttacg atgagactgc tatgtgaagc agcattaacc
    22501 cattttcagt ataacttttt tattgctaaa ataatcatat tattaaaatt cttatttata
    22561 tttttttaat aaaaagaaga acttgtcaca accgtttaca gatgttatta tgtgtaagat
    22621 attgtattaa tcgataagta caaaaaagga gcggaaaatg acacagcaat caatgttact
    22681 aagtattgag caagtaaata aatcctatgg taaaaatcag gttttaagta atatctcttt
    22741 tgatatttac aaaggagaaa tttgcggcct tgtgggtcaa aatggtgctg gaaagacaac
    22801 gttaatgcgt attttatctg gcctaattgg aaaagattca gggcaaatca aacagttaca
    22861 accctatcga atgggatcaa ttattgaatc accgacactt tatcccaata tgacagctca
    22921 tgataatctt tactatgcag cgttgcaatt gagattagct gatgcaaaag agcgcataca
    22981 tgaggttttg gaacttattg ggttagaaaa agtgagcaaa aagaaaaagg tgaaagatta
    23041 ttctttgggg atgcgccaac gtttggctat tggtctttcg atactagatt ttccagaatt
    23101 tttaatctta gatgaaccca ttaatggtct tgatccagca ggaatcaaag aaatgcgtca
    23161 gattattttg aacctaagag attgctatgg tattacgatt ttgatttcca gtcatatttt
    23221 atcagaatta gacttagttg ttgatcgtta cgtgattatg cataaaggca aagttattaa
    23281 gagtatggac aaagcagagc ttaaagctca agtaaaagta caaattgccc ttcatacaag
    23341 cgatgatcag ctggtaaaaa atagactttt agaactgggg cttagagttg agactgatgg
    23401 tcagatgcta ctaataaaac caactttatc agtaatggag cttatcaaaa ttgtgctaga
    23461 tctgcctgtt gagatttttg acatttatca tcatcaagtg agctttgaac attattacct
    23521 cgatttgtta ggtaaagatg cagcgtcacc attaatttag aaagaggttt ttatgatttc
    23581 ctatcttaaa gcagatttat ttcggatacg ccaagagcgt ttagctctat tttcttgtat
    23641 gctacttgtt gtcattgctg ttggtgtagc ctatagtttt cgcaagacac ctaaccaagt
    23701 catcatgata caaatcatta atattattac tgattttgtg acgcttttct ttttaacacc
    23761 agcccgtatc ttttttagtg atgactataa ctaccgtacg attaataatg ttgtggtcaa
    23821 acaacaaaat cgtactttaa ttataatcta taaactgcta gttagttttg gcttatctct
    23881 tttatttatt agtttaactt atcttcttag tgctttagca tttcatttat ttactggtca
    23941 agcatactat ggaatagtca tagatcggat gattaaacaa ctgccttttt atattgctat
    24001 tattgctttg tgtcaatttt tgtttaatat acttcgtaaa acttatcagt caatcacagc
    24061 ttatatactt tatgtccttc tttttgataa agtatctgct actttagcaa accatttttt
    24121 taagttagat attagcccag ttttgatgtt taaacaccta tccttgtctc ttggtgataa
    24181 acacctttct agtactagca tattagtggc ctttggttta actctttgtt atcttttagg
    24241 caatgtcttt cttttttcca aacgagaact aaaataaagg gtaatatgtg ctttgtttat
    24301 tttaaaagac aaagaagttc ttagaaaaag gtcgtcttca tcattactaa aaaaggatga
    24361 gcaagtagtt ttgttattat tcaagtaagc tcatacttaa tgagataaaa taaagacttg
    24421 tttacccggt atctgattgc taagagttgg atttattgga atactggtct caaactgtta
    24481 tagaaatgat tatgagtgca caatctcata gtttgaaaca tagttgttta ttatagacat
    24541 ttaggagatg tcctaattta aagcttaaag tactagaaat gtcccactaa tatgttttag
    24601 tgggacattt ctacaggaag ctagattatc cttcaatata atcttttagc tcttgaccag
    24661 taagtccggc gttgagagca attaataact ttagacgggc ttttggagca ttcaattctt
    24721 ttacaaacat aacacctgct tcttgtagca tcgcaccacc accttcataa gcatagacag
    24781 gttctgccat accattgaaa caacgggaaa caagaacgac aggaatacct aaagtaatta
    24841 agtgctcgat ctccccaact gctaatggtg ggacatttcc tgcccctaga gcttcgataa
    24901 ctagaccttg aatgctacct ggagttaata gactcaagat actaccatcg cccatacctg
    24961 cgtatgcttt gataatgggg atggttccag aaatgcatct caagtcaaaa cgaatacgag
    25021 gttcagcagt tttgaaaaaa agtaggtcat ttttcatgat gataccaagc ggtccgtgtg
    25081 tgggtgtttg gaaagttgaa atattggttg tatgtgtttt agtaacatat ttagcagcat
    25141 ggatttcatc gttcataaca acaagtaccc ctttttcttt agctttatca ctacttgcaa
    25201 ctctaagtgc ggtaaggtaa ttatatatgc catcactacc aacttcatta gaagacctca
    25261 tagcacctgt taggacaaca ggtatattag taggcaatgc catggtatct aaaaaataag
    25321 ctgtttcttc taaggtatct gttccatgtg tgatgactat accatcaaag acatcagttc
    25381 tttcttgaat atgatgataa atagataaca tgtggtgagg cgtgatgtga ggactcggaa
    25441 gatttagaaa atcactaacg gtaagctgaa tatcttgggc tgcagcatgt attttcgtca
    25501 taggattatc ttgatttgga acaaccctac cagagttatc agcttgcata gaaatggttc
    25561 caccagtgtg taagactagg atttttttca tttgattgtc aaacttcctt ttatttatga
    25621 tataatctat tctatcataa acacaggagg caaaagaagg ttttgacagt aaaagcagtc
    25681 ttttttgata ttgatgggac ccttctcaat gatcgtaaaa atatccaaaa aacaacgcaa
    25741 aaagccatcc aacaactcaa aaaacaaggc attatggtcg gtttagcaac tggtcgtgga
    25801 ccgggatttg tgcaaccttt tctagaaaat tttggattag attttgccgt tacttacaat
    25861 gggcaatata tcttaacaag agataaggtc ttatatcaga atcaattgcc aaagtcgatg
    25921 atttacaaag tgattcgcta tgccaacgaa aaaaagcgag aaatttctct tggaacagct
    25981 tctggattag caggatcgcg tattattgat atggggacta gcccatttgg acaagttatt
    26041 agtagttttg tgcccaaaag ttgggcaaga acagttgagg gtagttttaa gcatttgatt
    26101 cgccgaatca agcctcagag tttcagaaat ttagtaacta ttatgcggga gcctatttat
    26161 caggttgttc tggtagcgtc acaagctgaa actaagaaaa tacaagagaa gttccctcat
    26221 ataaaaatta caagaagtag tccttattcc ttggatctga tctctgttga tcaatcaaaa
    26281 ataaaaggaa ttgaacgttt gggagagatg tttggatttg acttgtctga agtgatggct
    26341 tttggagatt cagataatga ccttgaaatg ttatcaggag ttggtatcgg tgttgctatg
    26401 ggaaatgctg aaactgtggt aaaagatgga gcccatttca ctaccgactc taataataat
    26461 gatgggattt ccaaagcctt agctcactac ggtctcatcc attttgatgt agagaagagc
    26521 tttaaaagcc gtgatgaaaa ttttaataaa gtcaaagatt ttcatcgtct gatggactca
    26581 gatactattg aaacaccgcg tagctatacc atttcagaag ctggttatag gtcaggcttc
    26641 aaagtagaag aaattgttga gtttctttac gcagcaagca agggagacca acaacaattt
    26701 acacaggcta ttttcgattt gcatggtgct gttgaccaag cagctaataa ggtacaagct
    26761 aaaaaacatg ttgaaacgcc cctgattggg caagttgatg ccttagcgga tttgctttat
    26821 tttacttatg gttcttttgt actgatggga gtagatccac aacctatatt tgaagcagta
    26881 catgaagcaa atatggctaa aatttttcca gatggcaagg cacactttga ccccgttacc
    26941 cataaaattc aaaaacctga ctattggcaa gagcgtcacg cacctgaagt agccataaaa
    27001 aaagagttag acaaacaatt acaaaaatca ctacaacgat tcgataaata gaggtttact
    27061 cttttaaata tcaagaaata aaaagatatt gactagcctt agctcagcac aaaaaaacaa
    27121 gcaatttcgt tgtagatgct gcttgttttt tggtgtttga aaattgtaat agactaaagt
    27181 gttttagtgc tatctcggac tacaagtaag tcaatcttag cgtgtcgcat aatgtactca
    27241 gaagatgagc caataaggag gcgttcaaag gtattaagac cagtagctcc taccataatt
    27301 aaatcagcgt tctcacgatc tggaatatcg tgagctagta agtttttggg attgccaaat
    27361 tcaataattt gtttgatgtt tgtaattcct gctatctgtg cttgtttttc aaaatcatct
    27421 aaaacgtcct tagcttcttg ttctaatttt tcatagatgt aagtatcaaa agtagctacg
    27481 ctttgcaagg cacgggtgtc aataacgtgt actagtagta aggtagcgtc gttacgtagt
    27541 gctacgttaa cgcctttatt gaaagctaat tcagactcat aagagccatc aatagcaacg
    27601 aggatacgtt tatattttag tgacatagaa ctgtcctcct ttaacagttt gacaagaata
    27661 gcttttattc tttttttcat tatactcttt tttgagtcat tttgaaagcg atccattatt
    27721 ttggctaaac taaagactgt attatcgtct cataagacct gtgttaaaac gattagctgt
    27781 cctaagcttt gatggataaa ttctttctat tttaagtatt ttggtttata atatggaata
    27841 cgaggtgtat gagactatga aaattattga aaaatcatca aaattagaac atgttgccta
    27901 tgatattcga gggcctgttt tggatgaagc taatcgcatg attgcgagtg gtgaaaaaat
    27961 tctgcgttta aatactggca atcctgctgc ctttggtttt gaagcaccag atgaagtgat
    28021 tcgtgattta attgtcaacg cgaggctaag tgagggctat tctgacagta aagggatttt
    28081 ttcagcgcgt aaggctatca tgcaatattg ccaactcaag ggcttcccag atgttgacat
    28141 tgaagatatt tatttgggaa atggcgtgtc agagttgata tccatgtccc ttcaagcttt
    28201 gctagataat ggcgatgaag ttttagtacc aatgccggat tatcctttat ggacggcttg
    28261 tgtgagtctt ggtggaggaa aagcggtcca ttatctttgt gatgaagaag ctggttggta
    28321 tcctgatatt gctgatatca aatcaaaaat cacgagtcgc accaaagcta ttgtggtcat
    28381 caatcctaat aacccaacag gagcccttta tcctaaggag attttagaag acatcgttgc
    28441 tttggcaaga gaacaccagc tgattatttt tgccgatgaa atctatgatc gtttggtgat
    28501 ggatggtaaa gagcatattg ctattgcaag tttggcacct gatgtttttt gtgtatctat
    28561 gaatggtctt tccaaatctc atcgtattgc tggttttcgt gttggatgga tggtcttatc
    28621 aggtcctaaa gaccatgtta aaggttacat tgaagggtta aacatgcttg ccaatatgcg
    28681 gttatgctcc aatgttttag cccaacaagt tgttcaaact tccttaggag gtcatcaatc
    28741 agttgatgca ctgttactgc caggagggcg tatttttgag caacgtaatt ttatttatga
    28801 agctattaat gctatccctg gcttatcggc agttaaacct gaagcaggtc tttatttatt
    28861 tccgaaaatt gatcgtcaga tgtatcgtat tgatgatgat gaagaatttg tcttacagtt
    28921 attaaagcaa gaaaaagtga tgttggtaca tggacgtggt tttaactgga aagatccaga
    28981 tcacttccgt attgtttacc taccaagtgt agaagaatta gaagatgtcc aagaaaagat
    29041 tacacgtgtc ttataccagt atagacgtta atgcaatgct catctttgat taataaaaat
    29101 aacgtttctt aaaagaatgt gaccctatgc tctaacaagc tagtgcttat ctcctttaaa
    29161 tgaagtgata ataaggcatc aatagttgga attgtagtaa gaatagaaaa ttttctgata
    29221 attccttgca aaagcgagtt agctttgtta taatgaaagc gcaaataatt caaaggaaaa
    29281 ggaaaaaatg cctaacttat tagaaaaaac tcgtaaaatc acatctattt tgcagcgttc
    29341 cgtagatagc ctagaaacag aattaccgta taacacaatg gcatctcgcc tagcagatat
    29401 cattgactgc aatgcttgta ttatcaatgg cggcggcaca ctgcttggtt atgccatgaa
    29461 atataaaact aacactgatc gtgttgagga attttttgaa gctaaacaat ttccagatac
    29521 ttatgtaaag gctgctagcc gagtttacga tacagaagct aacctttctg tcgaaaatga
    29581 attgactata ttccctgttg agtctaaaga cacttatcca ggaggtctaa cgactattgc
    29641 gccgatttat ggtggaggga tgcgccttgg atcactcatt atctggcgta atgacgatga
    29701 gtttagtgat gatgatttga ttttggttga gatctcaagt actgttgtgg ggattcaact
    29761 attaaatctt cagacagaaa acttagaaga caccatccgt aaacaaacag cggtcaacat
    29821 ggcaattaat acgctttctt attcagaaat gaaagctgtt gcagcaattc ttggagagtt
    29881 agatggtaat gaaggacgat tgacagcttc tgttattgct gatcgtattg gtattacccg
    29941 ttctgttatt gtcaatgctc tgcgtaaact agaaagtgca gggattattg aaagtcgttc
    30001 tcttggtatg aaagggacat acctcaaagt tatcaacgaa ggtatttttg ctaaattaaa
    30061 agaattttaa atagaaaaag acaacatctc ttttcaagag atgttttttg ctgttaatgg
    30121 tagatgcaaa tagatgtctt gatacctgta ataaaaactg atacaatgcc atatataaga
    30181 gataagggga gaggtttccc tgtttaggag atagtgatga gagcattgat ttcaattgat
    30241 tacacaaatg attttgtagc agatgatggt aaattaagtg caggtaaatc tgcccaggct
    30301 attgccacta aaatagcaga ggtaactaaa acggcatttg atcaaggaga ctatattttt
    30361 tttgctattg attgtcatga tcaaaatgat ccttggcatc ctgagagtaa gttatttgca
    30421 gcacataata tcaaaggaac tactggacgc catctttatg gtccactagc tgaagtttac
    30481 agttatatga agcaacatcc cagagttttt tggattgata agcgttatta ttcagctttt
    30541 tcggggactg atttagatat tagactacga gaaagaggta tcactcaatt agtgttaaca
    30601 ggagttttat ctgatatctg tgtcttacat actgctattg acgcttatca tcttggttat
    30661 caattagaga ttgttaaatc ggctgtcgct agccttacaa aagaatctta tgagtggtct
    30721 ttggctcact ttgagcaggt tcttggtgct aagcttatta acgattacta aaagatccta
    30781 ccaaactatt gattcagtga ctaagagttt ggtaaccgta atcattgctc tatgtggttt
    30841 taccgtatgt atgggttagg tagatgaggt atgtgacatg ctggtcagaa ttagtcctga
    30901 tgattcttgt gctgtctaaa aggcaactaa actttggact gtgtcagata gaacacatgt
    30961 ttttttgaag aagacaggat tttagctgtt tgagaaatga tttgagttga tggtttagag
    31021 gatcgtctca cagccattag acaaactcta gtcttttcag cattagaata taaataaagc
    31081 ttcaaactct tgaatgctag aggctgtacc atttttatct taggcttatc acatcacatg
    31141 ttgtcacaat cgttgaggat ttggtaaatt aggataaagc acccctttat taccttcgta
    31201 aacatattac cgacttgaaa gaacgttact ttttctgtga tgttgtagga aaatgcttgg
    31261 aagaatacgt tgacaggggt atttttgata aggagccgtt ccagaccttt gatcagaaag
    31321 ggattggccg tctctttagc ccgttcggtt aagcctgagt tgaaacttgg tatttgtggg
    31381 gaacatggtg gcgatcctgc ttccattgac ttttaccaca gccaaggcct gacctacgtt
    31441 tcttgttcgc catttagagt gccgcttact cgcttggcgg ctgctcaggc tgccatcaaa
    31501 gcttcaggcc acagtcttac ccaagacaaa tagagtttag tttgtcacac ttcctgacta
    31561 aggttttctg accttagtca ggaagttttt tgctgtatag ctttctagaa ttgcattagt
    31621 cacctatggt aaatgggcgc aattgtgata taataattga gactatgtaa aaacgatgtg
    31681 aggagttttt ccgctacctt tggagaatag cagagctgtt ctttggaatt ggaatacttc
    31741 tgatcatctt ggatgggaga aatcatgaaa atttctgaag aagaagctcg ccatgtcgcg
    31801 aagctatcaa aattatcatt ctcagaaagt gagacaacaa cctttgcaac gaccttatcc
    31861 aaaatcgttg atatggttga attgctgaac gaagttgaca ccgaaggtgt cgccattaca
    31921 acaaccatgg cagataagaa aaatgtgatg cgtcaagata ttgctgagga aggaacagac
    31981 cgtgccttgc tctttaaaaa tgttcctgaa aaagaaaatc actttatcaa ggtacctgct
    32041 attttggatg acggaggaga tgcctaatgt cttttaatca taaaaccatt gaagagttgc
    32101 atgacctcct tgtagctaag gagatttccg caaccgaatt gactcaaaaa acacttgaag
    32161 acatcaagtc tcgtgaggag gctgtcgggt cgtttatcac tgtttcagaa gaagctgctc
    32221 ttaaacaagc tgcggcaatt gatgccaaag gtattgatgc tgataacctc atgtcgggta
    32281 ttccactagc tgttaaggat aatatctcaa ccaagggcat cctaaccaca gccgcttcaa
    32341 aaatgttgta taactacgag ccgatttttg atgccacatc agtagcaaat gcctacgcta
    32401 aagacatgat tgtcattgga aaaaccaaca tggacgaatt tgccatgggt ggttcaacag
    32461 aaacctctta tttcaagaaa acgaaaaatg cttgggacca cacaaaagta cctggtggct
    32521 catcaggtgg ttcggcaaca gcagtcgctt caggacaagt ccgtttatct cttggttcag
    32581 acactggtgg ttctattcgg cagccagctg catttaatgg tgttgtcggt ttgaaaccaa
    32641 cctatggcac tgtttctcgc tatggtctca ttgcttttgg aagttcactt gatcagattg
    32701 gaccttttgc tccgacagtt aaagaaaacg cccaattatt aaatgtgatt gcaagtagcg
    32761 atgtcaagga tgccacttca gcgcctgtcc gcattgcaga ctacacatct aagattggtc
    32821 gtgacattaa aggcatgaag attgcccttc ctaaagagta tcttggcgaa gggattgatc
    32881 ctgagattaa ggaaacggtt cttgcggcag ctaaacaatt tgaagcctta ggagctactg
    32941 ttgaggaagt tagtcttcca catagcaaat atggtgtggc tgtttattat attattgctt
    33001 cgtcagaagc gtcttcaaac ttacaacgtt ttgatggcat tcgctatggt ttccgtgctg
    33061 acgatgctaa aaacttggat gaaatctatg ttaacactcg tagccaaggc tttggtgatg
    33121 aggtgaaacg tcgtatcatg cttgggacct ttagtttgtc atcaggttat tatgacgctt
    33181 atttcaaaaa ggccggtcaa gtgcgtacct tgattattga agattttgac aaggtctttg
    33241 cggattatga tcttattcta ggcccaacaa caccgacggt tgcttttggc ttagacacac
    33301 tcaaccacga tcctgttgcc atgtatttgg ccgacttatt gaccattcca gttaacttgg
    33361 ctggtctgcc aggaatttcc attccagcag gattcgtcga tggcttgcca gtagggcttc
    33421 aattaattgg tccgaaatac gctgaagaaa ccatctatca agcagcagca gcctttgaag
    33481 cggtgacaga ttatcacaag caacaaccga ttatttttgg aggtgacaag taatgaattt
    33541 tgaaaccatt attgggcttg aagtccatgt ggaattaaat accaattcca aaattttctc
    33601 accttcgtca gctcactttg gtgaggatcc aaatgccaac acgaatgtga ttgattggtc
    33661 tttcccaggg gtcttaccag tcatgaacaa aggggtgatt gatgcgggca taaaagctgc
    33721 gcttgccctc aatatggaca tccataaaga gatgcatttc gaccgcaaaa attatttcta
    33781 cccagataat cctaaagctt atcaaatttc acagtttgac gagccgattg gctacaacgg
    33841 atggattgag attaaattgg aagacggttc aaccaagaaa attcgtattg aacgcgcaca
    33901 cttggaagaa gatgctggta aaaacactca cggcacagat ggttattctt atgtggacct
    33961 taaccgccaa ggagttcctt tgattgagat tgtgtcagaa gccgacatgc gttctcctga
    34021 agaagcctat gcttatttga cagctctgaa agaaatcatc cagtacacag gcatttctga
    34081 cgtcaagatg gaagaaggct ctatgcgggt ggatgccaat atttcccttc gtccttacgg
    34141 gcaagaacaa tttggaacaa agactgaatt gaaaaacttg aactccttct caaatgtccg
    34201 taaagggctt gaatttgagg tggaacgtca agccaaactc ttgcgttcag gtggggcgat
    34261 acgccaagaa acgcgccgct atgacgaagc gaataagggg actattttga tgcgggtcaa
    34321 agaaggggca gcggactatc gttatttccc agaaccagac ctacccttgt atgagattga
    34381 tgatgcttgg attgacgaga tgcgtgctca attacctcaa ttcccagctc aacgtcgtgc
    34441 taaatacgaa gaggaacttg gtctctcagc ttatgatgct agccagctaa cagctaccaa
    34501 agccttgtca gatttctttg aaactgcagt aagtcttggt ggcgatgcca agcaagtctc
    34561 taactggttg caaggagaag ttgcccaatt cttaaacgca gaaggcaaaa ccattgaaga
    34621 aatcgctctg acaccagaaa atttggtgga gatgattgct atcattgctg atggtacaat
    34681 ctcttctaaa atggctaaga aggtttttgt gcatttggct aaaaacggtg gctcagcacg
    34741 tgcttatgtg gaaaaagcag gactcgttca gatttctgat cccgctgttt tggtaccaat
    34801 tattcaccaa gtctttgctg ataatgaagc tgcagtggct gatttcaaat caggcaaacg
    34861 taatgctgat aaggctttca caggcttctt gatgaaggca accaaaggtc aagctaatcc
    34921 acaagtcgct caacaattat tggcacaaga attgcaaaaa ttgcgagatt gatagttgct
    34981 gatagcaata agttaaagta ttttgtctct gaaaaaaagg cggtcggtct ttcacaggct
    35041 gcctttttgt atgtcatgtt agtgaccgcc tataaaaaga ccatcatcta tgctaaaata
    35101 agactgacag gtattgcttt agaaccttca ggagtttctg acgggtattt aatgaaccat
    35161 cacgtttgtc cacattatta ttagcgatta gctgtgtgat aaagtttgtt aagttattta
    35221 gtgtgtgaag acttctagtc gttctcggtc tatctatctt aatagaagga attgtttttt
    35281 ctattttact caggcacgga aacacttact aaagaagaag gtaaaggaat caactaaagt
    35341 taagaacatt taatggatag cagttcttct aaaaggattt tacaagaaag gaggtgttaa
    35401 cgatgatatg gtctttaatt gtaggtgcct ttatcggcat gatagcaggt aaaatgactg
    35461 aaaaaagcca gtccatgggt tgttttacta atattatcac aggtctcata gggtcagctg
    35521 ttgggcaaag gctttttggg gattggggaa ttcagttggc aggtatggct attttcccgt
    35581 ccattttggg agcagcaatt gtaattgtac ttgtttcagc tatttttgga aaaaggtatt
    35641 aaaaaaaggc tgcatggaag tttccaagca gccttttttg caacataaaa tgtcttatct
    35701 ttttattggt tgcatcctca ttataagata gttgtgtgaa aatttaatat cgttttcttg
    35761 acaaatagtt agattttgtt actgttttga catcattttg taacgtcagt ttagttgtag
    35821 tgtgggtctg ttgttatata tatttgagcg aaacacacaa atgtaattgt ttggaaattt
    35881 atgtgatgat ttgaattgta gcaatagcat tgtttttaat aaaatagaca ataagtcagt
    35941 ggtctatttg atctcaaata gccatcagga agtgttttgc aagtgttttc tttaaatgct
    36001 ataataatta ataagacagt ttaggaggtt gaaatgacaa aaacaaggtt gtatattgcc
    36061 agacatggaa aaacgatgtt taataccatt ggtcgcgctc agggttggag tgacacacct
    36121 cttacaaaaa aaggagaaga aggtattcga gaacttggcc taggtttaaa agatgcaact
    36181 ataccattta aggctgcgtt ttcaagtgat tcaggtcgaa ccatgcagac aatagaaatt
    36241 attttaagag aatcagaaaa cgaatttttg ccttatacta aagataatcg tattcgtgag
    36301 tggtgttttg ggagtctaga agggacttat gattcagaat tatttctagg tgtccttcca
    36361 agaacaaaag cctttgaaaa tcgtgataac ctacgtgatg ttccctattc tgaactggca
    36421 gaaagcatcg tggaagtaga taccgctaat tgggcagaac catgggaagt tcttcgtaag
    36481 cgcatttggg aggggtttga agctattgcc ttgtccattc aaaatgctgg cggcggaaat
    36541 gctcttgtgg ttagccatgg tatgacaatt ggaacgtttc tgtggttgat tgatccagat
    36601 cgagacaaac agtatattga taatgggagc gttacagttg ttgaatttga tgatggccaa
    36661 tttacgataa agactattgg agatatgagt taccgctacc gcggtcgtga aataatagaa
    36721 gaacggtcag atgaaatata attcgttaaa ctatttagtg caattgctaa ttgttattgt
    36781 ttttttagga gggctttacc tctttataaa accagaagag tcagtaacac caactcagtt
    36841 gaataaaaaa gaaatccaac aaaaagatat caaaaaaacg gaccgtttac gagctttacc
    36901 taaggtatct gttgaagatt gggagttggt tttagtaaac cgcgatcaca ttacaaaaga
    36961 aatgagtcct gaattagctg acattaatgg tatttctgtg gataaacgca ttgagcaagc
    37021 cactagtgac tttttagcag cagcacaagc tatcgactcg caagaacatc ttatctctgg
    37081 gtaccgttcc gtagactacc aaacagagct ttaccaatct tctattaaaa aagagatggc
    37141 taacgatcca acattgacac aagaagctgc ggaggctttg gtgcaaacct attcacagcc
    37201 gccaggggct agtgaacatc acactggtct tgctattgac atgagcactg tcgatacttt
    37261 aaatgccagt gatccatcag tagctaaagc agttcaaaaa attgcacctg attatgggtt
    37321 tgtcttgcgc tttccagaag gaaaaaagac tagtacaggg gttgattatg aggattggca
    37381 ttaccgctat gtaggcaagg cgtctgctcg ttatatggct cagcacaacc taacgttgga
    37441 agagtacatt gctgctttaa aggagaaacg atgagaaaac gactaaagtt tccgtatttt
    37501 ttaacgcttc tagcttgctt tttattgctg cttgtttgtc ccttactgag tagccaaagg
    37561 atagctagtg cagataaaga agtaagggtg aactacagcc aaaaacaatt tattacaaaa
    37621 atgggtaaag aagttaaacc attggcaaaa tattatggca ttagaccatc tattttgatt
    37681 gctcaaattc ttttggaaac ccatgatgga aaaacattac tagcgtctaa gtatcataat
    37741 ctttttagca agaaagcaac tccaggacaa gcagccatta ccctaaagtc ccctaaacaa
    37801 accaaccaaa acgtgagata tgctatttat aaagatgacg ctagtgcaat tagagattat
    37861 ttacgaatgc ttcggcaggg caaagaagtc gataagcgtt tgtatcgtaa tcttgctaca
    37921 gaaaaaggct ataaagcacc agctaaaagt ttacaaaagt atttgcatta tactgataaa
    37981 acctatgcta ggcgactaat tcaggttatt gaaagtaacg atttaacaaa ctatgactga
    38041 aattagcact ctttataaaa gagtgctaat ttttttgagt ttttttcttg acatgtctcc
    38101 cataaagagt ataatagaat tataaattag cagtctacta aatagagtgc taaacgaaag
    38161 ggagtggttg gtttgtgatt acacaacgtc aaaatgatat tttgaattta atcgttgagc
    38221 tatttacaca aactcatgaa ccagtagggt ctaaagcttt acagagaacg attgattcta
    38281 gtagcgccac tattcgtaat gatatggcaa aactagagaa gttgggattg ttggaaaaag
    38341 cgcacacttc tagtggacgg atgccaagtc cagctggttt taagtatttt gtagagcact
    38401 ccctcaggct agatagtatt gatgagcaag atatttatca tgtcataaaa gcttttgatt
    38461 ttgaagcatt caaattagaa gacatgcttc aaaaagctag tcacatttta gcggagatga
    38521 cgggctatac atccgttatt ttagatgtgg agccagctcg tcaacgttta acagggtttg
    38581 atgttgttca gcttagcaac catgatgctc tagcagtcat gactttagat gaatctaagc
    38641 cagtgacagt gcagtttgct attccaagaa actttttgac acgtgacttg atagcattta
    38701 aagctatcgt agaggagcgc ttgctggata atagtgtcat agatattcat tataagttga
    38761 ggacagaaat tcctcagatc gttcaaaagt attttgtgac tacggataat gttttacaac
    38821 tatttgatta tgttttttca gagctgtttt tggaaacggt atttgttgct ggcaaggtaa
    38881 attctttaac gtattcagat ctatcaacgt atcagttttt ggataatgaa cagcaagtag
    38941 caatttcttt gaggcaaagt ctgaaagaag gtgagatggc ttctgtccaa gtggcagata
    39001 gtcaagaagc cgcacttgca gatgtgtcag ttttaactca caaatttttg attccttacc
    39061 gtgggtttgg actcctgagt ttgattggtc ctattgatat ggattatcgt aggtctgtta
    39121 gtttggtaaa cattattgga aaggtattgg ctgctaaatt aggtgattat tatcgatacc
    39181 taaattctaa ccattatgaa gttcactgat aatggcagta ttcaataagt tatttaaaag
    39241 gaggcactca gtgtcagaag aaattaaaaa agacgattta caagaagaag tggaagccac
    39301 tgaaactgaa gagacagttg aggaagttat tgaagaaact cctgaaaaat cagagttaga
    39361 acttgcaaac gaacgtgctg atgagtttga aaataaatac ttgcgagctc atgcagaaat
    39421 gcagaatata cagcgtcgct ctagtgaaga acgtcaacaa cttcaacgct atcgttctca
    39481 ggatctggca aaagcaatct taccatcgct tgacaatcta gaacgtgcct tggctgttga
    39541 agggttgaca gatgatgtta aaaaaggttt agaaatgact cgtgatagcc ttattcaagc
    39601 actaaaggaa gaaggcgtag aagaagtaga agtcgactcg tttgaccaca actttcatat
    39661 ggcagttcag accctcccag ccgatgatga acatccagcc gatagcattg cagaagtctt
    39721 tcaaaaggga tataaattgc acgagcgtct gctaagacca gcgatggtag ttgtttataa
    39781 ctaaggtaaa tgagttttcg tttttgtccg taatgacagt aaactagata gcaagttaga
    39841 agctatttcg cttgctgatt aaactatagt gattgcttag aattggaagt aaaataattc
    39901 gagtgcttac taagataaat tgaaataaaa agtaataaag tattataaaa taagaggtat
    39961 taacatgtct aaaattattg gtattgactt aggtacaaca aactcagcag tagcagttct
    40021 tgaagggact gaatcaaaaa tcattgctaa cccagaaggc aatcgtacaa ctccttcagt
    40081 agtatcattc aaaaatggtg aaattatcgt gggtgatgct gcaaaacgtc aagcagtgac
    40141 aaacccagaa acagtaatct ctattaaatc taaaatggga acttctgaaa aagtttctgc
    40201 aaatggtaaa gaatatactc ctcaagaaat ttcagcaatg attcttcaat accttaaagg
    40261 ttatgctgaa gactatcttg gagaaaaagt agaaaaagca gttattactg ttccagctta
    40321 cttcaacgat gcacaacgtc aagcaactaa agacgctggt aaaattgcag gtcttgaagt
    40381 agaacgtatc gttaacgaac caacagcagc tgcacttgct tatggtatgg acaagactga
    40441 caaggatgaa aaaatcttag tttttgacct tggtggtggt acatttgacg tatcaatcct
    40501 tgaattaggt gatggtgtct tcgacgttct tgcaacagca ggtgataaca aacttggtgg
    40561 tgacgacttt gaccaaaaaa ttattgattt cttagtggct gaatttaaga aagaaaatgg
    40621 tattgactta tcacaagata agatggcact tcaacgcttg aaagatgctg ctgaaaaagc
    40681 taaaaaagat ctttcaggtg tgacacaaac acaaatttca ttaccgttca tcactgctgg
    40741 ttctgctggt cctcttcact tagagatgag cttatctcgt gctaaatttg acgatctcac
    40801 tcgtgacctt gttgaacgta cgaaaactcc agttcgtcaa gctctttcag atgcaggatt
    40861 gtcattgtca gaaattgatg aagttatcct tgttggtgga tcaactcgta tcccagcagt
    40921 tgtcgaagct gtaaaagctg aaactggtaa agaaccaaat aaatctgtaa accctgatga
    40981 agtggttgct atgggtgctg ctatccaagg tggggttatc actggggatg tgaaagacgt
    41041 tgtccttctt gacgtaacac cattgtcact tggtattgaa acaatgggtg gtgtcttcac
    41101 taaattgatc gaccgcaata caactatccc aacatctaaa tcacaagtct tctcaacagc
    41161 agcagacaac caaccagccg ttgatatcca tgttcttcaa ggtgaacgcc caatggcagc
    41221 agataacaag actcttggtc gcttccaatt gactgatatc ccagctgcac ctcgtggaat
    41281 cccacaaatt gaagtaacat ttgatatcga taaaaacggt attgtttctg taaaagctaa
    41341 agaccttggt acgcaaaagg aacaacacat cgttatcaaa tcaaacgacg gactttctga
    41401 agaagaaatt gatcgcatga tgaaagacgc tgaagctaat gccgaagccg atgcgaaacg
    41461 taaagaagaa gttgacctta aaaacgaagt tgaccaagct atctttgcta ctgaaaaaac
    41521 aatcaaagaa actgaaggta aaggctttga tacagaacgc gatgcagcgc aatcagctct
    41581 tgacgagtta aaagctgcgc aagaatctgg caaccttgac gacatgaaag ctaaacttga
    41641 agcattaaat gaaaaagcgc aagctttggc tgttaaaatg tacgagcaag ctgcagcagc
    41701 tcaacaagca gcacaaggtg cagaaggtgc acaagctaat gattcagcaa ataatgatga
    41761 tgttgtagat ggcgaattta cagaaaagta atgatttagt tatctagtaa cattaatatc
    41821 cgaattcaga ggttgcacca aacctctgtt tttggctaaa taaaatgtaa aaatgctgac
    41881 gtcaaaatat tttaagaaag gaatacaagt tcgattattc gaacacaggc taaagcgtgt
    41941 aaagactctt ttgcggattt gaacacgcct acgaccctgc gaaaaaagag aaatacctcc
    42001 tagatacgag agtatctttg tcggatttcc tatttttcat tgagtcgttt tacggcttgg
    42061 tatcttacat tatgaacaat acagaatatt acgatcgtct gggggtttct aaggacgctt
    42121 ctcaggacga tatcaaaaaa gcttatcgaa aaatgtctaa aaaatatcat ccagatatca
    42181 ataaagaagc tggagcggag caaaagtata aggatgttca agaagcttac gaaaccttaa
    42241 gtgattcaca aaagcgtgct gcttatgatc aatacggcgc tgcaggtgct caaggtggct
    42301 ttggtggtgg agcaggcggc tttggtggtt ttgacggagg tggctttggt ggttttgaag
    42361 atatcttctc aagcttcttt ggtggcggtg gaagccgaaa cccaaatgcc cctcgtcaag
    42421 gtgacgattt gcaatatcgt gttaatttat cttttgaaga ggctgtattt ggtgttgaaa
    42481 aagaagttag ctataatcgc gaagcaacct gcggcacttg tttaggctca ggtgcaaaac
    42541 ctggtaccgc tccagtaact tgtcgtaagt gccatggttc aggtgtaatg acaattgaca
    42601 cccaaacacc acttggtatg atgcgtcgac aagtcacctg cgatatttgt catggtagtg
    42661 gtaaggaaat taaagaaccc tgtcaaacct gtcacggtac tggtcatgaa aagcaagctc
    42721 ataaagtgtc tgttaagatt ccagctggtg tagagactgg tcagcaaatc cgtttacaag
    42781 gtcaaggaga agctggcttt aatggcggac cttatggtga tttatttgtt attttgaatg
    42841 tcttaccaag taagcaattt gaacgcaatg gttccactat ttactacaac ttagatattt
    42901 cttttactca agcagcctta ggagatactg ttgaaattcc aacggttcat ggcgatgttg
    42961 agatggctat ccctgctggg acacaaacag gtaaaacctt ccgcctcaaa ggcaaaggag
    43021 caccaaaact ccgtggtgga ggtcaagggg atcaacatgt taccgttaat attgtgaccc
    43081 caaccaaact caatgacgct caaagggaag ccttgcaagc atttgcagaa gcgagtggtg
    43141 aaaaaatgtt acatccaaag aaaaaaggtt tttttgataa agtgaaagat gccttagagg
    43201 atatttaaag atgtcaaacg tataatctca ggataatttt cccgaagtta tacgtttttt
    43261 tacgtcaaaa cagttagaga aaacgttttc ttttatagat atcgtttttt tctcttgtca
    43321 aaatggttta tttagtatat cataattagg taattgattt gattatcaaa tgattttaaa
    43381 gagaaataat ttcttagtca aataaattat cagaaaagag gattgttatg cagtttaaac
    43441 acatcatctt tgatgttgta gatgatcttg cgacattgac tttaaaccgt ccagaggttt
    43501 caaacggttt taatattcca atttgtcaag agattctggt agcattagca gaagttaaac
    43561 gcgatacatc tgtgcgtttt ttattaatta aagccgttgg aaaagtgttt tcagttggtg
    43621 gtgacctagt tgaaatgcaa gaagctgttg caaaagataa tgtgcagtcg ctagtcaaga
    43681 ttgctgaact ggtacaagaa atttcttttg caatcaagca tttgccaaaa ccagtcatcc
    43741 tttgcgccga tggagctgta gctggagcgg cttttaatat agctttagct gttgactttt
    43801 gtatcgctag tactcaaaca aaatttatcc aagcttttgt aaatgttgga ctagcaccgg
    43861 atgccggtgg tttatttcta ttgactcgtg cagtaggact taatcgtgca actcaccttg
    43921 tgatgacagg tgaaggaatt actgctgata aaggtctcga ttatgggttt gtctaccgta
    43981 cggcagaatc tgataaacta gacaaagttt gtcttcaact tttaaagcga ttgagacgtg
    44041 gctcttcaaa ttcttatgct ggaatgaaat cattggtctg gcaaagcttt ttcacaggat
    44101 gggaagatta tgctaaggca gagcttgcta ttcaagagga gctagctttc aaggaagatt
    44161 ttaaagaagg tgtcatagct tttggagaaa ggcgccgacc aaattttcaa ggtaagtaga
    44221 ctcatgcttg caaagtgctt ttaactctga tataatttga ctgtcaaagt tttattgtta
    44281 ggaggtattt gcttggaata tgacaaaatc aatcactact tggttgatat cttcaaccgt
    44341 attttggtaa ttgaagagat gagtttaaaa accagtcaat ttaatgatgt ctctttaaaa
    44401 gagatgcaca ctattgaaat tatcggcaaa tatgatcagg tgactccaag tgatatcgcc
    44461 agagaattaa tggtaacgtt agggacagta accactagct taaataaatt agaagcaaaa
    44521 ggttatatcg caagaacgcg atcacgctca gataggcgag ttgtttacct gtcattaaca
    44581 aaaaggggtc gacttttgga ccgcctccat gcaaagttcc ataaaaatat ggttggacat
    44641 gtcattgctg atatgagtga tgaagagatg caggcattgg tgcgtggact agggaatcta
    44701 catcaatttt tggaggattt ggtttaaatg attttttcta aaatcagtca ggtagcccac
    44761 tatgttccac agcagttagt gactaataat gatttagcta acataatgga cactagtcat
    44821 gagtggatct tttctcgaac aggaatagca gaacgccata tttctagaga tgaaatgact
    44881 agtgacctag ctattcaggt tgctgatcag cttttaactc agtctgggtt aaaagctgat
    44941 gctattgatt ttattattgt tgcaactatc agccctgatg ccactatgcc atcaacagct
    45001 gcgaaagttc aagcagctat tgcggctaca agtgcttttg cttttgatat gactgctgct
    45061 tgtagtggct ttgtctttgc cttggctatg gcagataagc ttattgcatc aggtgcctac
    45121 caaaacggaa tggtaatagg tgcagagact ttatctaagt tagtaaactg gcaggatcga
    45181 gcgacagccg ttctttttgg agacggagct ggtggcgtgc tactggaagc cagtaaagat
    45241 aagcatgttt tagcggaaac attacacaca gatggtgcga gatgtcagag tttaatttca
    45301 ggagaaacta gtctttcatc tccttactca attggcaaaa aagccatagc gactattcag
    45361 atggatggac gtgccatatt tgattttgcg attagggatg tgtcaaaaag catcctcaca
    45421 ctaatggcgc aatcagatat tacaaaagat gatatagact attgtttatt gcatcaagct
    45481 aatcgtcgta tacttgataa aatagcacgc aagattgatg ttcctcggga aaaattcctt
    45541 gaaaacatga tgcgttatgg taatactagt gctgctagta tcccaatcct cttgtcagag
    45601 gctgttcaaa agggacagat taggttagat ggtacccaaa aaatcttact ttccggtttt
    45661 ggtggaggtt tgacatgggg aagtctaatt gttagaatct agttattcat gtgcctagca
    45721 catttataaa aacaaattta tattttttaa ggagaaataa acatggcagt atttgaaaaa
    45781 gtacaagaaa ttattgttga agaacttggt aaggagacag aagaagtaac actagaaact
    45841 acatttgatg atttagatgc agattcactt gatgttttcc aagttatttc agaaattgaa
    45901 gatgcttttg atattcaaat tgaaacagaa gaaggattaa atactgttgg tgaccttgtt
    45961 gcttacgttg aggaaaagtc aaaataagga taaaagtgac aagtaacatc agaagtctat
    46021 gaaaagctta ctgctgttat gttcatgttc atcactaaaa aaaacaataa gtattaatca
    46081 aaccattagg gtgggattag actcttttta ggatctagct cgctctttct tcttactata
    46141 atagtttgat tatcaaatta ttctccttat tttttataga aagttattat gaaaacacgt
    46201 attacagaat tacttaatat tgattacccc atttttcaag gaggaatggc ttgggttgct
    46261 gatggtgatt tagcaggtgc agtttctaat gctggtggtt taggcattat aggtggtggc
    46321 aatgctccca aagaagtcgt taaagctaat attgatcgtg tcaaagctat tactgataga
    46381 ccttttgggg ttaatatcat gcttttatct ccttttgctg atgatatcgt tgatttggtc
    46441 attgaagaag gtgttaaagt agtaacaaca ggcgcaggaa atccaggaaa gtatatggaa
    46501 agactgcacc aggcgggtat aatcgttgtt cctgttgtcc caagcgttgc gctagccaaa
    46561 cgtatggaaa agctaggggt agatgctgtt attgctgagg gtatggaagc tggaggacat
    46621 attggcaagt taacgactat gtctttagta agacaagttg ttgaagcggt ttcgattcct
    46681 gtcattgcgg caggtggtat agctgatggt catggtgcag cagcagcatt tatgttagga
    46741 gcagaggctg ttcaaattgg aactcgcttt gttgttgcta aagaatccaa tgcccaccaa
    46801 aattttaaag ataaaatctt agcagcaaaa gatattgata cggtgatttc tgcgcaggtt
    46861 gtgggccacc ctgtccgttc tattaaaaat aaattgacct cagcttacgc taaagcagaa
    46921 aaagcatttt taattggtca aaaaacagct actgatattg aagaaatggg agcaggatcg
    46981 cttcgacatg ctgttattga aggcgatgta gtcaatggat ctgttatggc tggccaaatt
    47041 gcagggcttg tgagaaaaga agaaagctgt gaaacgattt taaaagatat ttattatggt
    47101 gcagctcgtg ttattcaaaa tgaagctaag cgctggcaat ctgtttcaat agaaaagtag
    47161 ataaacacat gaaggataat gataatgaca aagacagcct ttttatttgc cggtcaaggt
    47221 gcccaaaaat tagggatggc aagggatttt tatgataact ttgctattgt aagaaaaacc
    47281 tttgatcaag ctagtcaagt attgggatac gatttgcgcc gtttgattga tagtgacgag
    47341 ttaaaactta atcaaactag ctatactcag ccagctattt tgacatcatc aattgctatt
    47401 taccgtgttt tgggcttaca tcacgttaaa ccggatatgg tagctggtct ttccttagga
    47461 gagtactcag ctttggtagc atcaggggca ctctcttttg aagataccct atccttagta
    47521 gctaagagag ggcgcttgat ggaggaagca gcaccacaag gatctggaaa gatggttgcc
    47581 gttatgaata cagacgtgca agtcatcgaa gaagtctgtc aaatagctgc taaacatgga
    47641 gtggttgctc cagcaaacta taatactcct agtcaaattg taattggtgg tcagacagat
    47701 gctgtgaacg tggcagttga acttttaaag gaaaggggag ttaagcgttt aatcccttta
    47761 aacgtgtcag gtcctttcca cactgctttg ttagaaccag ctagccgctt gttggctaaa
    47821 gagttggaaa gatacaactt cagtgacttc aagattcctt tggttggtaa taccgaagct
    47881 aatattatgg aaaaagaccg tatcccagaa ctattagccc gtcaagtcat ggagcctgtt
    47941 cgtttttatg acagtgttgc gactttagta gaaagtggca taacacaatt cattgaggta
    48001 ggaccaggta aggttttgac aggttttgtg aagaaaattg ataaaaattt actatgtact
    48061 agtgttgaaa acatggtaag cttacgttta tttttagata gagaggacat tcatggaaat
    48121 taaaggaaaa aatatattta tcacaggctc aacgagaggt atcggtttag caatggctca
    48181 tcaattcgcc agtttaggag ctaatattgt cttaaatggt cgctctgcta tctctgagga
    48241 gcttgttgct agttttactg actacggcgt tacagttgtt accatctctg gggatgtttc
    48301 agaagcatca gaagctaaac gtatggtcaa tgaagctatc gaaagtttag gttcgattga
    48361 tgttttagtt aacaatgctg gcattactaa tgataaattg atgcttaaaa tgacggaaga
    48421 ggattttgag cgcgtcttga aaatcaattt gaccggtgct tttaacatga ctcaatcagt
    48481 gttaaaacca atgatcaagg cccgccaagg agcaattatt aatgtctcta gtgtggttgg
    48541 tttaacaggc aatattgggc aagcaaacta tgccgcttct aaggcaggaa tgattggttt
    48601 taccaagtct gttgcccgtg aagttgctgc tcgtaacatt tgcgtgaatg ctattgctcc
    48661 aggatttatc gagtccgaca tgacaggtgt ccttcctgaa aaaatgcaag agcaaatctt
    48721 aagtcaaatc ccaatgaaac gtattggaaa agctcaagaa gttgctcact tagcaagctt
    48781 cttggtggaa caagattata ttacgggaca agttatcgct attgatggcg gtatgacaat
    48841 gcaataagga gacgtagtga aatgacattt aaaagagtag tggtaacagg ttatggtcta
    48901 acatctccaa tcgggcatga tcctgaaacg ttttggaaca atctgaaagc tggccaaatc
    48961 ggtattggcc ctattactaa atttgacacg actgattatg cggtcaagaa tgctgctgaa
    49021 attcaggatt ttccatttga caaatacttt gtgaaaaaag atttgaaccg tttcgataga
    49081 tattctcttt atgctcttta cgcagcgaaa gaggctatta atcatgctga tttaaatatt
    49141 gagatgattg attccgatcg atttggcgtt attgtcgctt caggtattgg tggaattgcg
    49201 gagattgaag agcaggtgat tcgcttacat gaaaaaggac caaaacgtgt gaagccaatg
    49261 acgttgccaa aagcgctacc aaatatggct gcaggaaatg tcgccatgtc tttaaaagct
    49321 caaggagtat gtaaatcaat taatactgcc tgcgcttctt caaatgatgc gattggggat
    49381 gcctttcgtg ccattaaatt tgggactcaa gatgtgatga tagttggtgg gtcagaggcg
    49441 gccataacca agtttgccat tgctggcttc caaagtttaa cagctctctc aacaactgaa
    49501 gatccaagca gaagctctat tccatttgat aaagaccgaa atggttttat catgggagaa
    49561 ggatcaggaa tgcttgtttt agaaagccta gaacatgcgc aagaacgcgg ggcgacaatc
    49621 ttagcagaaa ttgtgggtta tggaaatacc tgcgatgctt accatatgac atctccaaat
    49681 cctgagggat taggagctcg taaagccatc cacttagcct tacaagaagc tggtattgaa
    49741 gccagtgcta ttaattatgt caatgcgcac ggaacttcaa ccccagctaa tgaaaaagga
    49801 gagagtcaag caattgtagc cgttcttggt aaggatgttc cggtgtcttc aacaaaatcg
    49861 tttacgggtc accttcttgg agcagcaggc gctattgaag ctattgccac tatcgaggct
    49921 atgcgtcata actatgttcc gatgacggct gggacacaag ctttatcaga ggatatcgaa
    49981 gcaaatgtca tctttggaga aggaaaagaa acagctatca actatgccat ttcaaatact
    50041 tttggatttg gtggtcataa tgctgtatta gctttcaaat gttgggagga gtaggttgaa
    50101 tattcaagaa atcaaagatt tgatggctca gtttgatacc tcaagcctac gtgaattttt
    50161 attcaaaaca aatgaaggtg agctaatctt tagtaaaaac gaacaacacc tcaatgcatc
    50221 gacatcaaat caagaacatg ctgtgcctgt accacaggta caacttgttc cgaatccaac
    50281 tgcttcggaa gcgtctagtc cagcatcagt taaggatgtt cctgtagagg agcaaccaca
    50341 agcagaatct tttgtagcag aaggtgatat tgttgaaagt cctttagtcg gtgtagccta
    50401 tcttgcagct agccctgaca agccaccatt tgtggcagtc ggagatactg taaaaaaagg
    50461 acaaaccctt gtgattatcg aagccatgaa ggtgatgaac gaagtgccag cgccatgtga
    50521 tggtgtgatt acagaaattt tggtatcaaa tgaagatgtc attgaatttg gacaaggatt
    50581 ggtgcggatc aaatgatgga tattagagaa attcaagcgg ctttgcctca tcgctaccca
    50641 atgttattgg ttgatcgtgt attagaagtg tcagacgacc atatcgttgc cattaaaaat
    50701 gtgactatta atgaaccttt ctttaacggg cattttcctc actatcctgt gatgccaggt
    50761 gttctgatta tggaagcttt ggctcaaaca gctggtgtct tagaattgtc aaaagaagaa
    50821 aataaaggca agttggtctt ttacgctgga atggataaag tgaagttcaa aaaacaagtc
    50881 gtccctggtg atcaacttgt gatgacagca acctttatca agcgtcgcgg taccattgct
    50941 gtcgttgaag ccagggcaga agttgatggc aagctagcag caagtggaac attaaccttt
    51001 gcctgtgggc aatgagaatg attgttctgc ggaggaagaa acccttatgt ttaaaaaaat
    51061 cttaattgct aatcgtggtg aaattgcagt gcgtatcatt cgtgctgcac gagaattagg
    51121 gatttccaca gttgcagttt attcggaagc ggataaggaa gctctgcaca cgatattggc
    51181 ggacgaggct atctgtattg gtcctgcccg ttccaaggaa tcctatctta atatgaattc
    51241 cgtcttgtca gcagctattg tgacaggagc gcaagcgatt cacccagggt ttggcttttt
    51301 aagtgaaaac tcaaagtttg cgaccatgtg tgaagagatg aatattaaat ttatcggtcc
    51361 atccgcatcg gttatggata aaatgggtga taaaatcaat gctcgttctg aaatgattaa
    51421 ggcaggagtg cccgttattc cagggtcaga tggtgaagtt tataacgctc aagaagcctt
    51481 agctattgcc aataagattg gttatcctgt catgctaaaa gcatcggcag gtggtggagg
    51541 taaagggatt cgtaaagtgg agacagaagc agatttggag gcggctttca atgctgccag
    51601 tcaagaagct ttgggagctt tcggaaatgg cgccatgtat ttggaaaaag taatttatcc
    51661 agctagacat attgaggtgc aaattttagg agacgcttac ggtaacatca ttcacttggg
    51721 agaacgggat tgctcgttac agcgtcataa tcaaaaagtc ttggaagaaa gtccatccat
    51781 tgctattggc aataccctca gacacgaaat ggggcaagca gcggttagag cagctgaagc
    51841 tgtagcctat gaaaatgcgg gaaccattga gtttttgttg gacgaagaca gcgagaaatt
    51901 ctatttcatg gaaatgaata cccgtatcca agttgagcat cctgtcaccg aatttgtgac
    51961 aggagtggat attgtcaaag agcaaatcaa aattgcagca ggtcagcctt tggcgattaa
    52021 tcaagaagac atcactatca cgggacatgc tattgaatgt cgtatcaatg ctgaaaatac
    52081 tgcttttaac tttgcaccga gtccaggaaa gattacagac ttgtatatgc caagtggtgg
    52141 tgtaggacta cgagttgatt cagcagttta caatggctat gccattcctc cttattatga
    52201 cagcatgatt gctaaaatca tcgttcatgg aagcaatcga tttgacgctt tgatgaaaat
    52261 gcaacgtgcc ttagttgaat tggaaattga aggcattatc accaatacag acttccagct
    52321 cgatttgatt tcagataagc gagtgattgc aggagattat gatacctctt tcttgatgga
    52381 aacgttcctg ccacattaca ataaagatta ggagaaaata tggctttatt tcgtaaaaaa
    52441 gataaataca tccgtattac tcccaataat tccctcaaag gatctgtcag ccataatgtc
    52501 ccagaagttc cagatgaact ctttgcgaaa tgcccagctt gtaagcacat gatttataag
    52561 aaagatttgg gattggcaaa gatttgccca acctgttctt acaattttag gatttctgcc
    52621 caagagcggc tgaccttaac agttgatgaa ggctctttcc aggaattatt tacaagtatt
    52681 gaaaccaagg atccgttacg ttttccaggt tatcaggaaa aattacaaaa agccaaggaa
    52741 acaactggtc tgcatgaagc tgttttgaca gggaaagcta tggttaaagg acaacaaatt
    52801 gcacttgcta ttatggattc tcactttatt atggctagta tgggaactgt tgttggggaa
    52861 aaaattaccc gattgtttga attggctatc gaagaaaacc tgccggtggt gatttttacg
    52921 gcttcaggag gtgcccgtat gcaagagggt atcatgagcc ttatgcaaat ggccaaggtc
    52981 tctgcagctg taaaaagaca ttctaatgca ggcctctttt acctcactat tttgacagat
    53041 ccaactactg gtggggtaac agctagtttt gccatggaag gggatattat tttagcagaa
    53101 cctcaatcct tggttggatt tgctggacgc cgtgttattg aaaccacagt acgtgagaat
    53161 ttgccggatg attttcaaaa agcagaattt ttacaagacc atggttttgt ggatgctatt
    53221 gtgaaacgaa cagagttgcg agacaagatt gcccacttag tagcatttca tggaggtggc
    53281 caatgacaga tgtatcaaga gttttaaaag aagcgcgtga tcaagggcgt ttaacaactt
    53341 tggattacgc caaccttatt ttcgatgact ttatggaact gcatggcgat cgccattttt
    53401 cagatgatgg tgctattgta ggtggcttag cttatttggc gggacaacct gttacggtca
    53461 ttggtattca aaaaggtaag aatttacagg ataatttggc aaggaatttt ggccagccca
    53521 atccagaagg ttatcggaaa gccctacgtt taatgaaaca ggcagaaaaa tttggacgac
    53581 ccgttgtcac ctttatcaat actgcaggag cttatccagg agttggtgcg gaggaacgag
    53641 gacagggtga ggccattgct aaaaatttga tggaaatgag tgatctcaag gttcccatta
    53701 tcgccatcat tattggtgaa gggggctctg gtggtgcatt agccttagcg gttgccgatc
    53761 aggtctggat gcttgaaaat accatgtatg cggtccttag cccagaaggc tttgcttcta
    53821 ttttatggaa agatggttca agagcgacag aggctgctga attgatgaaa attaccgcag
    53881 gcgaattgta tcaaatgggc atagtagatc gtattatccc agaacatggt tatttttcaa
    53941 gtgaaatcgt tgacatcatc aaagctaacc tcatcgaaca aataaccagt ttgcaagcta
    54001 agccattaga ccaattatta gatgagcgct acaaacgctt tcgtaaatat taacaacatc
    54061 cttttggagt ctccctacgt gtttatcaag ctaagggagg cctttccaaa agggttttta
    54121 actagtcttg taaataagga aatgggtata ctttgataaa ctagtgtgaa gaaaagtgtt
    54181 gttaaagggg caatgtagtg agtttcaagt gatgtggtgg ctctttaaaa tagtaaaccc
    54241 cagaaatcac tttaatgatc tctggggtga gtttattatt taggtgaaat gacggtttcg
    54301 ccacccatgt aagggcggag tacttcaggg atggtaacag aaccatcttc attttggtag
    54361 ttttctaaaa tggctgctac ggtacgaccg acagcaagtc ctgacccatt aagggtgtga
    54421 agaagtttga ccttaccgtc agcctcatca cggtaacgga tttgagcacg acgtgcttgg
    54481 aaatcttctg tatttgaaca tgatgaaatt tcacgatagg tattttgtgc aggaatccag
    54541 acttcaaggt cataggtttt agcagctgag aagcccatgt ctccagtaca aagggaaatg
    54601 acgcgatatg gcaaaccaag tttttgaaga atattttcag cattagcagt cattttttct
    54661 agttcttggt aagattcttc tggtttagca aatttaacca tttcaacttt gtggaattgg
    54721 tgtaggcgga taaggccacg tgtgtcacga cccgcagaac ccgcttcaga acgaaaagat
    54781 gggctcattg cagtaaagta aattggcaat tctttgccgt ctaggatttc cccacggtaa
    54841 taattggtca aaggcacttc agcagttgga atcaggacaa agttggtatc tgctaattca
    54901 aaggtgtcct ctttgaactt tggatattgt cctgtaccaa acatggaatc gtggttaacc
    54961 atgtaaggtg tgatgatttc ttgataacct tctttgatat gttcatccag catgaagtta
    55021 taaagagcac gctccaaacg agcaccaaga tttttataga ataagaaacg agcaccagtt
    55081 acctttgccc cacgttccca gtctaaaata tcaagatctt cgcctagatc ccaatgagcc
    55141 ttaacttcga agtcaaagtc acgaggcgtt ccccaacggc gaatttcgac attgtcttct
    55201 tcgtcagctc caacagggac agaatcgtga ggagtattag gtaaaacagt gatgatgtca
    55261 gtaacttgct ggtcgatagc tactagttga ttgtcaatag ttttgatatc tgcagagact
    55321 ttttgcatat cagcaatttg ttgagtggca tcttcttttt gacgttttgc ttgggcgatg
    55381 gctgcagaag caatattacg ctctgctttt aattcttctg attgcactaa tagagcacga
    55441 cgtttttcat caagttcttt aagatgggtc agggtgtctt cagaaacacc gcggtttttt
    55501 aatttggcag cgacagtgtc aaaatcggta cggatacgtt taagatctag catacagaag
    55561 ctcctttaag atagttatta gtagctgtca gtgaaacaag gctaagtaag acagttggat
    55621 cttgtgattc tctgacgata gaaaaagcac agcttatgaa gatgctggag cgctttggcg
    55681 cggttccatc cagcttcaca atctgtgcac ttaatctata ttttattgat gacacggtag
    55741 aatttcatat cattttccct atctgttcac agcatccaca gactctctga atgaaaaaaa
    55801 ggtattactt atccgttagg actattataa caaaaaacaa gaaaaagtaa actcaaatga
    55861 gggaatcttt tccactaaat agcaatttag agctactatt ctttgctttc ttgaaatgat
    55921 aaatcggcac tgcgcattat ttttttaatg tcgaaaaaat ttgttaataa aggtttgcat
    55981 caaggttggt aatgtaatca ctttctcatt accaatatgt tgacgagtga tttttaagat
    56041 ttttccagag tctccagaag caaataaaaa cttgccttgg tccgtaaaaa tttcaaagtg
    56101 gcggctaact ttttttcttg aaacattagc cccgatgtga ttgatagctg tccaaggaat
    56161 ttgaatataa tcattcacat ttttatcgtt gtaaaattca agggccttgt caccgagtaa
    56221 aatatgacca actttgcctc ccattccaag gtaggagaca gcttttgtct tgtattcgac
    56281 actagtgttt aaagattgag ccattttagt tcctttcatt gcgtagttgt agttattata
    56341 ccatatttta agtgggattt tcctgacata aagcataaaa aagaagcctg agttcatctc
    56401 agacttccat tggttgatta cataatacct gcaaggtgag caaggatacc aaatgcaaac
    56461 aagccgatga tgattgcgat tggagaaact tttttcttca ataaccacat gcaaaggaat
    56521 gtaaggagta atccagctaa acctgggata agagagtcca attgtccttg aagggtttga
    56581 gcttggactt tatcaaggct cataccacca atagcgttgg caaggatagt tttcaactca
    56641 gcacctttaa cagcgccatc tgggaagacc acataggcac catctgacaa ttgttttcca
    56701 ggaaggtcga tagtaaagtt gatagatacc caacgttgaa caagcactgc aaggataaac
    56761 attccaagga ttgaagcacc tttagtaatg tcttggagga taccgcctga catgtcttta
    56821 gtaatttcag aaccagcttt gtaaccaaat tcttgagtat accataagaa ggccatacgg
    56881 ataaggttcc atccaaagaa gaacagaagt ggaccaacga tgttacctgt tgaagccaaa
    56941 gaagcaccaa gtgccccaag gataggacga accgtaaacc agaatactgg gtcaccgata
    57001 ccagcaagag gtcccatcat accgatttta accccttgga tagccttgtc atcaattggc
    57061 gtaccatttg cgcgttcttc ttcaagggca agtgttacac caatgattgg agctgcaacg
    57121 tatgggtgag tgttaaagaa ttccatgtgg cgctcaagag cagctgcacg gtcttcagga
    57181 gacgtataca attttttaag agcagggata agtgcataag cccagcccat gttttgcata
    57241 cgttcgtagt tccaagaacc ttgtaagaat tgtgaacgcc accaaacacg ttgacggtct
    57301 gatttagtta atttaatttg ttcagtcatt tgttcttacc cctttctagt agtcttctaa
    57361 gatgtcgccg attggatcag ctgaacctgc tgcattacca ccgttaccac cttttttaga
    57421 aagattaagg tagatgaagg caattgctac accgataata cccatagcga taagggttaa
    57481 ttggctgata gctgcaagag caaaacctag ggcgaagaat ggccatactt cacgagtagc
    57541 catcatgttg ataaccatag cataacctac agcaacaacc atcgcaccac cgatttgcat
    57601 accttcgttc aaccagtttg gcatagcatt caaaacagat tgtacggctg aagtaggaac
    57661 agcaacaaga agagctgctg ggacagcgat acgcaatcct tgaagaagaa gggcaataaa
    57721 gtggaagcgt tcaatacctg cgaaattacc ttcagcggca gctttgtcac cagcgtgtac
    57781 aagggcagtt gacaaagtac gaacgatcat tgtaaggaaa agaccagcaa cagcaagtgg
    57841 gatagctgtt gagtaagcaa aggtaattcc tttttgagta aagtcgccac ttttgaccat
    57901 gatgatagca gctgcaacag aggcaagggc agcatcaggg gcaacggcag caccgatgtt
    57961 tgcccaacca agagcaagca tttgaagtgt accaccaagg atgacaccag cttcaaggtg
    58021 tccagttaca agtccaatca aagtacaggc aacaagtggt tggtgcattt gaaattggtc
    58081 gaggatacct tcaagaccag cgaagaaggc aatgataacg accaaaattg cagaaataat
    58141 cgaaatatct gacatgttgt aatcctttca aagtatagta aacgaggcct ttttgctaat
    58201 aagttagcta gtcatcgttt taaaacctta tagcgtttga aacttattta atgttagctt
    58261 tttggataag ctcaaataag tttttcttag aatcgttagg aactttacgg acgtcgaatg
    58321 taacacctag gtcgcgtaat ttttcaaaag tagcaacgtc ttctttgtcc attgataaaa
    58381 cgttgttaac cattgtttta ccagttgagt gagccattga accaacgttc aattcgttga
    58441 tttcaacacc accttcaacc gctcgaaggg catcttgagg ggtttggaaa agaataagtg
    58501 cgtgtgtgtt accaaaacga ggatctttag aagcttcaat caatttgcta attggaacaa
    58561 cgttagcttt gacaccacca ggtgcagcct gtttgatcaa ttgtttgcga aggtcatctt
    58621 gagcaacctc atcagaagca acaatgatac gatcagcctt agaagctgga gtccatgctg
    58681 ttgcaacttg accgtgtaaa agacgagtgt caacacgagc aagattgatc tttaatttac
    58741 catctccgat gacagttcca gcaggaatag ctccttgaag tgcgttaaca actttttcag
    58801 ttgcagcagt ttcttcaact gggttaaggt cttcaggaag ggctttgata ccgtccttag
    58861 attccttaat gatgtttgca gcaacttgtt caacacctgc accagcatcc ataagacgtt
    58921 cagtgtaagc ttgaataagc ataggcaagt tgagaccagt gatgatcgcc attttgcggt
    58981 caggattttc acctgccaca cgactagctt ggttaaatgg agaaccactc caaaggtcag
    59041 ctagcacaag gatttcatca tcagcatcaa attgttggat agcattgttg aagtgtccat
    59101 acaaatcatc tggtccttcg tttggcatga aagtaacaac ttgaactttc tcttgttcac
    59161 caaaaatcat agaaccagat tgatgaatac cttcagcaaa tttaccatgg ctggcaataa
    59221 taataccgat acccattctt gttcttcctc ctttaaaata ttcttcgttg aaatgaagaa
    59281 gaataatctc cttccaaaac taaattttgg caattctttt tcacttaaac gaaattcaag
    59341 aacgtttcta ttttaaaacg ttttcactaa aaaatcaagc cctttcggaa aaatagtaca
    59401 aaacatgaaa aaattttcag aattcagtat taaataggca gattattgat attaaaggtg
    59461 ttttcaagct gtttaaaaca aaaatatgct gaaaaatgtt gaaattatta aaatgatttt
    59521 cattttttga tagctgttga cagaagaaaa accaccattt tctgaaaaat agtggtttcg
    59581 tttgctacta caagaaaagt tcttccaatt ttttggcaac accgtcttct tcgttggaaa
    59641 aattaagctg ctgatcagca taaggcagta gcacagggct agcatttttc atagcataac
    59701 cagttccagc aaaagctagc atctcagtgt cattgtgttc atcaccaaag gcgattaaat
    59761 cctttttatc catattgtaa attccaagga gatagttcag agcgtaagct ttattgacat
    59821 tcttagacga aatttcaaga atgttgagag ggcctcccca agaatcaatt tcaatttcat
    59881 ctttgaagaa agcacgcatg tttttagcta aggcatactt gtcttcgtga tgcgtttgca
    59941 tcaataaggc gtttggattt cgagtgattt tggtaatctc taatgccatg tcctgagtaa
    60001 tttcatcaac accaaagagt tgcgggtcga tactttctgg attggtcatg gtgatgtaga
    60061 cattttttcg atactcactg gcaataaaat ccatctggaa atcatcctga tattttagta
    60121 accttagtag gtagcctttg tccaaagtga cattatgttc ataggcccat ttttgttcag
    60181 gcatgtgggt caaggcccca ttaaaagtaa tgattggggt ctttaaattg agctgcagat
    60241 agtagtccag tgccatgcgg taaggacggc ctgttgaaat aatgacgtgg tggcccttgt
    60301 cttggacagc tttgatggtt ttttgagtat aggtagagat ggtgttgtca tgatgtaata
    60361 aggttccatc caaatcaatg gcaatcaatt ttttagtcat aattaaatta tagcaaattt
    60421 tgctccaaga taaagagaaa agataagtta agcgtattca ttagtaaaaa agataggata
    60481 actgtttttt taatgaataa atgaagttat agacgttgaa aagtgaacaa aaaaatggta
    60541 acatagtgtt attgttcatt ttaatacgaa caataacaat aataatgagg tgtcaaaaag
    60601 acttatctag tgataagcgc tttttgattt tacctactat ttaattaagg agactccatt
    60661 tcaatggaaa agttttttaa gttaagcgaa aatgggacaa ctgtctcaac tgagattatg
    60721 gctggtttaa cgaccttttt tgccatgtcc tatattttgt ttgttaaccc aagtatttta
    60781 ggtgcagcgg ggatgccctc taatgctgtc tttttggcta cgattatcgc agcagccata
    60841 tcaaccttaa ttatgggact atttgccaat gtgccttatg cgttggcacc aggaatggga
    60901 cttaacgctt ttttcactta tacagttgtt tttgctttag ggttttcatg gcaagaagcc
    60961 ttggcaatgg ttttcatttg tggattattc aatattttta ttaccgtaac caagtttcgt
    61021 aaaagtatca tcaaggcgat tccagttagt ttacagcatg ctattggtgg gggaattggt
    61081 gtctttgtag cttatttagg atttaaaaac gcaaatatca ttactttttc tatctctgct
    61141 gaaaatatag taatggtaaa tggtgttgaa ccggctaaag catcggctaa aacatttgca
    61201 gatggtctat tatttgtaga cgccaatggt ggagttgtac ctacgatttc tagttttacg
    61261 gattccggtg tattacttgc tatttttggt ttacttttaa cgacagctct tgtgattcga
    61321 aattttagag gtgctatttt aattggtatt gtcgcaacaa ctcttgtagg tattccttta
    61381 ggaatagtgg atatgtccaa cctcaatttt gggatcagcc atattggtga agcttggact
    61441 gaattaggta caactttcct tgcagctttc gatggtttga gttctctttt tagcgattca
    61501 agtcgtttac cgctagtttt catgactatt tttgctttta gtctatcaga tacttttgac
    61561 acaattggta cctttatcgg aactggtcgt cgaacaggta ttttctctca agacgatgag
    61621 aatgctttgg aaaatagtat aggctttagt tcaaaaatgg atcgtgcgct ttttgcagac
    61681 gctatcggta cttctattgg ggctttggtt ggaacttcaa atacgactac ctatgttgaa
    61741 tcagcagcag gaattgctga aggtggacgt actggactaa cagcagtctc caccgcagta
    61801 tgcttcttat tatcaatatt gctattaccg cttgtaggta ttgtcccagc tgctgctacg
    61861 gctccagctt taattattgt gggtgtcatg atggtgtctt cttttcttga tgttaattgg
    61921 agtaaatttg cagatgctct tccagctttt tttgcagctt tctttatggc gctgtgttac
    61981 tctatttcct atggtattgc cgctgccttt attttctatt gtctagtaaa agttgttgag
    62041 ggaaaaacaa aagatattca ccctattatt tggggagcaa ccttcttgtt cattgtaaat
    62101 ttcatcatat taactatctt ataaccaaat aatttactta ttttgctact aactgccgct
    62161 tctttaattg attttaacct ttaatataaa gaagcgtttt tactttacga ataccttatt
    62221 ggagtcatct tctaggtagt gcttggaagt atgttttttt tctgatataa tgttgctatg
    62281 ttttatagtg aaaatgaata tactcttaaa gcttacggag aaaccctagg tacttatttg
    62341 tctattggag atgtgattgt attgtctggc gacttaggtg ccggtaaaac aacattggca
    62401 aaaggtattg ctaaagggat ggggattagt caaatgatca aaagcccaac ttatactatt
    62461 gttagagaat atgaaggacg tctacctctt tatcacttag atatttaccg tgttggtgat
    62521 gatccagact ctattgattt agatgatttt ctttttggta atggtgttac cgttattgag
    62581 tggggagaat tattaggaga gggattatta caagactatt tgcaaattac cattactaaa
    62641 cgagacaaag gtcgtcaact agacttgttg gcgcatggtg agcgctctcg tcagcttctg
    62701 gagataatgt cacatggttc agcaaagtct cgttattgaa gaaggcgtac ctcaagatgc
    62761 aaaagctgta ctttccttag tgacggcagc tgccaaagaa acagatttta taacaggcgt
    62821 agagactata ttagaggcta ctcctcaaga attgtcaggc tttttatcaa gaagtcaaac
    62881 ctcttttatt gatttttgcc ttctagctag attagatgaa aaagtagtag ggcttttaaa
    62941 tctttctggg gaagtattat cccaaggcca agctgaagca gatgtattta tgttagttgc
    63001 taagacttac cgtggttatg gtatcggtca attattgtta gagattgctc ttgattgggc
    63061 tgaagaaaac ccttatatcg aaagccttaa gttggacgtt caagtaagaa atactaaggc
    63121 tatttacctg tataaaaagt atggttttcg tattgaaagt atgcgaaaaa acgacatcaa
    63181 atcaaaaaat ggagatgact tagatgttta tcatatgcgt aaatctgtaa gtcagtaagt
    63241 gaatcgatga aaattggaaa aaaaatagtt ttaatgttca cagctattgt gttaacaact
    63301 gtcttggcat taggtgtcta tctaactagt gcttatacct tctcaacagg agaattatca
    63361 aagaccttta aagatttttc gacatcttca aagaaaagtg atgccattaa acaaacaaga
    63421 gctttttcta tcttgttgat gggtgttgat acaggctctt cagagcgtgc ctccaagtgg
    63481 gaaggaaaca gtgattcgat gattttggtt acggttaatc caaagaccaa gaaaacaact
    63541 atgactagtt tagaacgaga taccttaacc acgttatctg gacccaaaaa taatgaaatg
    63601 aatggtgttg aagctaagct taacgctgct tatgcagcag gtggcgctca gatggctatt
    63661 atgaccgtgc aagatctttt gaatatcacc attgataact atgttcaaat taatatgcaa
    63721 ggccttattg atcttgtgaa tgcagttgga gggattacag ttacaaatga gtttgatttt
    63781 cctatctcga ttgctgaaaa cgaacctgaa tatcaagcta ctgttgcgcc tggaacacac
    63841 aaaattaacg gtgaacaagc tttggtttat gctcgtatgc gttatgatga tcctgaggga
    63901 gattatggtc gacaaaagcg tcaacgtgaa gtcattcaaa aggtattgaa aaaaatcctt
    63961 gctcttgata gcattagctc ttatcggaag attttatctg ctgtaagtag taatatgcaa
    64021 acgaatatcg aaatctcttc tcgcactatc cctagtctat taggttatcg tgacgcactt
    64081 agaactatta agacttatca actaaaagga gaagatgcca ctttatcaga tggtggatca
    64141 taccaaattg ttacctctaa tcatttgtta gaaatccaaa atcgtatccg aacagaatta
    64201 ggacttcata aggttagtca attaaaaaca aatgctactg tttatgaaaa gttgtatggg
    64261 tcaactaagt ctcagacagt aaacaacaac tatgactctt caggccaggc tccatcttat
    64321 tctgatagtc atagctctta cgctaattat tcaagtggag tagataccgg ctcatcttat
    64381 cagagtgcta gtacagacca ggactatact gcttcaagcc atacgccaac tacgccgtct
    64441 tcttcatcgg atgctttagc agctgatgag tctagctcat cagggtctgg atcattagtt
    64501 cctcctgcta atatcaaccc tcagacctaa cgttctgagg gtttttatgt ttaaaatatc
    64561 cgattttgaa tataaaaaag acaggtatca aagatgatag ctgtctgtga tttcaaataa
    64621 agtgtatgag ggagataata ctatctggaa tggttgttag ttaaaatgac ggtttggcaa
    64681 gtaggtgatt cactagaaat tagctcacta ttttcttgat atttggccat acgattttgg
    64741 atttcagtga ggtgtgcttg tgtctcttgg ttaaagtagc gccatttatg ggccaagtcc
    64801 cgactaatat tttgaattac ttttccttga ctgtgaatga ggtcaaggtt tgctttaatg
    64861 ttatctaaat caagttgggc agcatctttt gcttgtctac tgatggataa cagctctttt
    64921 atggtacaac gtttttgata tgctttgtaa gcggtaaaag ataataatcc gacggctatc
    64981 acggttttaa ctttcatgaa ctaacctcct gattgatttg tcgggcgagc ttttccaaga
    65041 caggaaagtc aggctcatga gtggtgtatt gaattgagat accgtcttct tcattataac
    65101 gaggaacgag atgaacatga gcatggaaaa cagtttgtcc cgctaaggct tcgttattgt
    65161 taatgatatt catggcagta gcgccagtag ctgactggat agcacgagca attttaggaa
    65221 tacgagcaaa gagatgacta gctgtctcag cagtcatctc tagtaagtta cgaacatgtt
    65281 gcttaggaat gactagagta tgcccttttg tagtttgtga gatatctaaa aaggcgagga
    65341 cctgctcatc ttcgtagact ttcgaagagg ggatatcccc ttgaatgatg ctgcaaaaaa
    65401 tacaattttc cattttatat caccttctct acttgaattt gttataatat attataagta
    65461 tatcaaaaac tagaaagaaa ttatatgtta aacattaaaa atctaacagg aggatatcac
    65521 aatatccctg ttttaaacga tgtctcattt tctgtagaca atggagagtt agtcggtttg
    65581 atcgggctaa atggtgctgg aaagtcaaca actatcaacg aaattattgg ctttttgaag
    65641 ccttatcaag gaagtatttc tattgatggt ttaactctag cagaaaatgc agttgcctac
    65701 cgtcaaaaaa taggttttat cccagaaact cctagtctct atgaagaatt gaccttatca
    65761 gagcatatca acaccgtagc tatggcttat gatatcgact tagaagtggc acaaaaacga
    65821 gcacaacctt tcttggaaat gtttcgttta actgacaaac ttgaatggtt tcctgtcaat
    65881 ttttctaaag ggatgaaaca aaaagtcatg attatttgtg cctttgtcat tgatccaagt
    65941 ctatttattc ttgatgaacc attcttaggg ttagaccctt tagctatttc agacttaatc
    66001 caaacgctag aagtcgaaaa agcaaaagga aaatctattt tgatgagtac acatgttttg
    66061 gactctgctg aaagaatgtg tgatcgcttt gtgattttgc atcatggaca agttcgtgcc
    66121 caaggaactc ttgctgactt gcaagaagct tttggtgacc gttcagcgag cttaaatgat
    66181 atctatcttg cattaacaaa agaggattaa gtatgaaagc attatttctg aaacgccgtc
    66241 aagactttca aaaacaacaa aacaaatacc ttcgttacgt cttaaatgat cactttgttt
    66301 tggtgttgat gtttttatta ggctttgcta tggtgcagta tggtcagcta ctaaaccatt
    66361 ttccaacgaa tcatctacct attcaggttt gtcttgggat attgatccca ttattgctca
    66421 gtatgggatc tatagcaacg tatctagaag aggcagatca gcatttctta cttccaaaag
    66481 aagaagaagt gatttcttat atcaaacaag ctgagcgact ctcttttttg ctttggggaa
    66541 ctttgcagac tgctgtattg ctctttttat atcctatttt tagacgtcta ggtctgtctt
    66601 tatttatttt tattatctta gttttgatat tattggcact aaaaagagta gtgcttagtc
    66661 gaaagacaag atattttttg agaggaaatc gtttagattg ggcaaaagct gtcgcatttg
    66721 aaagcaatcg caagcaaagt attttaaagt tttattctct ttttacaaca gtcaaaggaa
    66781 tttctataaa agtgaaagaa agagcttatc taaatcctct tttaaaatta gttaagcaaa
    66841 ctccttcaaa cctttggctg tccctttatg ctagagcctt tttacgtagt tcagattatt
    66901 tgggcttatt tttacgacta atgctattaa gctcattatc tgtatttttt atccataatc
    66961 tttatttgtc agtaagttta gcactcattt ttaactattt agttgtattc caattacttt
    67021 cactttatta tcactacgat taccattata tgactagtct ttatcctgaa aatagtcgga
    67081 gcaagaagaa aaatatgtta agttttttac gaggtttaag ctttttaatg ttaatcgtca
    67141 atatgctgtg ttgctcatca gcgcccaaag cactcatttt gattgtaggt atggttttca
    67201 tagcttgtat atatctacct tataagttaa agaagatcat tgactaagta gcctaaaaca
    67261 agtaaaatag gggtaaacga ctataaaaga tagaaaagga gggcgataag gtgacaacga
    67321 cggaacaaga acttaccttg actcccttac gtgggaaaag tggcaaagct tataaaggca
    67381 cttatccaaa tggggaatgt gtctttataa aattaaatac gacccctatt ctacctgcct
    67441 tagcaaaaga acagattgcg ccacagttac tttgggccaa acgcatgggc aatggtgata
    67501 tgatgagtgc ccaagaatgg cttaacggcc gtacattgac caaagaagat atgaacagta
    67561 agcaaatcat tcatattcta ttgcgccttc acaaatctaa aaaattagtc aatcaactgc
    67621 ttcagctcaa ttataagatt gaaaacccat acgatttatt ggttgatttt gagcaaaatg
    67681 cacccttgca aattcagcaa aattcatact tacaagctat cgttaaagaa ttaaaacgga
    67741 gcttaccaga gttcaaatca gaagtagcaa cgattgtgca tggagatatt aaacatagca
    67801 attgggtgat tactactagt ggtatgattt ttttagtaga ttgggattct gttcgtctga
    67861 ctgatcggat gtatgatgtt gcttacctgt tgagccacta tattccacgg tctcgttggt
    67921 cagaatggct gtcttattat ggctataaaa ataatgacaa ggttatgcaa aaaattattt
    67981 ggtatggtca attttctcac ctgacacaaa ttctcaagtg ttttgacaag cgtgacatgg
    68041 agcatgtgaa tcaggagatt tatgccctca gaaaatttag agaaatattt agaaagaaat
    68101 aatgcgagtt agaaaacgaa aaggtgctga ggagcactta gctaataacc cacactatgt
    68161 gatattaaat ccagaagatg ccaagggccg ttggcatgac gtttttggaa atgaccgtcc
    68221 cattcatatc gaagttggtt caggaaaggg cggatttatc acagggatgg ccttgaaaaa
    68281 tcctgatatt aactacattg gcattgatat tcaattatct gtcctaagct acgccttgga
    68341 taaggtatta gcttcagaag tacccaatgt caaacttctg cgagtggatg gatcaagttt
    68401 aaccaactat tttgaagatg gtgaggtgga tatgatgtat ctcaactttt cagatccttg
    68461 gccaaaaacc aagcatgaaa aacgtcgttt gacctacaag gatttcttag acacttacaa
    68521 gcgtatcttg ccagaacacg gtgaaattca ttttaaaacc gataatcggg gcttgtttga
    68581 gtacagttta gctagttttt ctcaatacgg catgaccttg agacagatct ggcttgactt
    68641 acacgctagt aactacgaag gaaatgtcat gacagaatac gaagagaagt tctttaataa
    68701 aggtcaagtc atttaccgag ttgaggcaaa tttctagtag tattttgttt caaaatatga
    68761 taacataaag gagttgatgc agtattaact tctttagtaa tggagaggtc gcatagtggc
    68821 ttagtgcgca cgcttggaaa gcgtgtagtc cttaacgggg ctcgggggtt cgaatcccct
    68881 cctctccttg tgagtaaaac aaagtgcata tccttgcatt cctcttaaat tatggtataa
    68941 taggcttaaa taatacagaa aggtggtggg atatgtctca ccacttttgt attaagtgga
    69001 ttcccaagga ccaattatct tagagaagag cataaaaata gaggaggtaa tcaaaatagc
    69061 aaatacatct attatcgata tcgtaaccaa gacagtaaca cctgagatta aggcacctta
    69121 tgaattagta gacgttgagt atgacaaaat gggtagtgac tacatcctta gtattttagt
    69181 cgataaggaa ggtggaatca cagtggaaga tacatctgac ttgacgaata ttattagtcc
    69241 gctgttagat acgattgatc cagatccttt tccaaaccaa tatatgctag aagtctctag
    69301 tccaggtttg gagcgtccgc tcaagacagc tgacagttta aaagcagcag ttggctctta
    69361 tatcaatgtg agtctttatc aagctattga taaggtgaaa gtttttcaag gagacttatt
    69421 agcctttgat ggtgaaacgt tgaccattga ttatcttgat aaaacacgtc ataagatcgt
    69481 gaatatacca tatcaagcag ttgctaaggt gagaatggct gtcaaattat aacttttatt
    69541 tcaaagcact aaactttttt aggaacgctt aagcaaattt gaaacaacac ctatagagtt
    69601 tatacaagct aaacaaaagg aattaaggga gcctttaggg ggctattgat cgtattaatt
    69661 taagaaagga tttttgccaa gctttacaag caaggtagat aacattatga gcaaagaaat
    69721 gctagaagcc ttccgtattt tggaagaaga aaaacacatt gataaagcag atattattga
    69781 cgcagtgaca gaatcattaa agtctgccta taaacgtcgc tatggtcaat cagaatcatg
    69841 tgttattgaa tttaatgaaa aaacagctga ttttcaagta ttcacggttc gtgaggttgt
    69901 tgaagaagtt tttgacagcc ggcttgagat tagtttgaaa gatgctcttg ctatcagttc
    69961 tgcctatgaa ttgggtgata agattcgctt tgaggaatct gtcaatgaat ttggacgtgt
    70021 cgcagctcaa tcagctaagc agactattat ggaaaagatg cgccgccaaa tgcgtgaagt
    70081 gatgttcaat gaatacaaag agcatgaagg tgaaattatg acaggaacag ttgaacggtt
    70141 tgatcaacgt tttatttatg tcaatcttgg ttccttagaa gcacaattgt ctcaccaaga
    70201 ccagattcca ggtgaaactt tcaaatcaca tgaccgtatt gacgtttatg tctacaaagt
    70261 tgaaaataat cctaaaggag ttaacgtctt tgttagtcgt agccatccag aattcatcaa
    70321 acgtattatg gagcaagaaa ttcctgaagt ctttgatggt actgttgaaa tcatgagtgt
    70381 ctctcgtgaa gcaggtgacc gtaccaaggt tgctgtgcgc agtcataatc ctaacgttga
    70441 tgccatcgga acaattgttg gacgtggtgg aagtaatatt aaaaaggtca tcagcaagtt
    70501 ccatccaaaa cgtgtagatg ctaagacggg gcttgaaatt ccagttgaag aaaatattga
    70561 cgtgattcaa tgggtagatg atccagctga gttcatctac aatgccattg ctccggctga
    70621 agtagacatg gtactttttg acgatgaaga tcttaaacgt gctacggtag tagttcctga
    70681 tagcaaatta tcattagcta tcgggcgtcg cggccaaaac gttcgtttag cggcacattt
    70741 gactggctac cgtattgata tcaaatcagc tagcgaatat gatcgtcttg aggcggaaaa
    70801 agaagcagca acagttgtgg aagagccagt tgttgacgat gttatgactg ccgaggaagt
    70861 gtaagaaaag aggtgtttga tgtctaaagt caaaaaaata cctttacgaa aatcacttgt
    70921 ttctggtgaa attattgcta aacgagatct tcttaggatt gtaaaaacta aggatggtca
    70981 agttttcatt gatccaactg gtaaacaaaa tggtcgtggt gcttatatca aattagacaa
    71041 tcaagaggcc ttaatggcca agaaaaaaca agttttcaac aggagtttct caatggacat
    71101 tccagaaagt ttttacgatg acttgattgc ttatgttgat cacaaaatca aaagaagaga
    71161 gttaggtctt gactaattta gaaagattat ctagtcttat tggtctagct caacgggctg
    71221 gaaaagtgat ttcaggtgaa gaactagtag taaaagctat tcaacaccag caggtaatat
    71281 tagtattttt agccaatgat gctggtccta atgtgacaaa aaaagtgaca gataaaagta
    71341 attattataa tgtagaagtc tccacagtgt tgaatgcact ggaattaagt gctgcactag
    71401 gtaaaccaag gaaggttgct gccgttgcag atgctggatt ttcaaagaaa atgaggactc
    71461 ttatggaata gatgaatagg aggacacaaa ttgtcaaaga aaagattaca tgaaattgcc
    71521 aaggaaattg gtaaaagtag taaagaggtg gttgaacacg ctaaatactt aggattagat
    71581 gtgaaaagcc atgcatcaag tgttgaagaa gcggatgcga aaaagattat ttcaagtttt
    71641 tcaaaggctt ctaaacctga tgtgacagct tctcaaacag tcaagcccaa agaagttgct
    71701 caaccctctg taacagtggt caaagaaaca ggttctgagc atgtagagaa aacacaggtc
    71761 tctaagccaa agagtcgtaa ttttaaagct gaaagagagg ctcgtgcaaa ggaacaggct
    71821 gctcgaaaac aagcaaatgg ctcttctcat cgttctcaag aacgtcgagg aggttatcgc
    71881 cagcccaata atcatcaaac taacgaacaa ggagataaga ggattaccca tcgttctcaa
    71941 ggtgatacaa acgataagcg tattgagcga aaagcatcga atgtatcacc tagacatgat
    72001 aatcatcagc tagttggtga tcgtaaccgt tcctttgcta aggagaatca caagaatggt
    72061 cgattcacaa accagaaaaa gcaaggacga caagagcctc aatcgaaaag tcctaaaatt
    72121 gatttcaaag ctcgtgctgc tgccttaaaa gctgaacaaa atgctgaata ctcacgccaa
    72181 agtgaaacac gttttcgtgc acagcaagaa gctaagcgtt tagctgagtt ggcaagacaa
    72241 gaagctaaag aagcggcttt aaaagcacaa gctgaggaga tgagccatcg tgaagcagca
    72301 ctgaaatcta ttgaagaagc agaaactaaa ttaaagtcat caaatattag tgctaaatct
    72361 actgctgata atcgccgtaa aaaacaagca cgtcctgaaa agaatcgtga gttaactcat
    72421 cattcacaag aaggacaaaa gaaaaataag aagagttgga atagtcaaaa ccaagtgaga
    72481 aatcaaaaaa atagtaactg gaataaaaat aaaaagacca aaaaaggcaa aaatgttaag
    72541 aatactaaca ccgctccaaa accagtaacc gagcgtaaat tccatgaatt gccaaaagaa
    72601 tttgaatata ctgaaggcat gacagttgcc gaaatcgcaa aacgtatcaa acgcgaaccc
    72661 gctgaaattg ttaaaaagct gtttatgatg ggtgttatgg caactcaaaa ccagtcttta
    72721 gatggggata ctatcgaatt attgatggta gattatggta ttgaggctaa ggctaaggta
    72781 gaagtagatg atgctgatat cgagcgcttc tttgaggatg aaaattacct caatcctgaa
    72841 aatattgtag aacgcgcacc tgttgtaact atcatgggac acgtagacca cggtaagaca
    72901 actttgcttg atactttgcg taactcacgt gtggcaacag gagaagctgg aggaattact
    72961 cagcatattg gtgcttacca gattgaagaa gctggcaaga aaattacctt cttggatact
    73021 cccggacacg cggcctttac aagcatgcgc gcacgtggag cttccgttac cgatatcacc
    73081 attttgattg ttgcagctga tgatggtgtt atgccacaaa cgattgaagc gatcaatcac
    73141 tcaaaagctg ctggtgttcc aattattgtt gctatcaaca aaattgataa accaggtgct
    73201 aatccagaac gtgttatcgc agaattggca gaatatggta tcatctcaac tgcttggggc
    73261 ggagagtgcg aatttgtaga gatatctgct aaatttaata aaaatattga tgaactatta
    73321 gaaacagtcc ttttggttgc tgaagtagaa gaattgaaag ctgatccgac ggttcgtgct
    73381 attggtactg tgattgaagc acgcttagat aaaggtaaag gtgctatcgc tacgcttctt
    73441 gtccaacaag gaactcttca tgttcaagac ccaatcgttg ttggtaatac ctttggtcgt
    73501 gtccgtgcca tggtaaatga ccttgggcgt cgtgttaaat ctgcggagcc atcaacacca
    73561 gtttccatta ctggtttgaa cgaaacacca atggctggtg atcactttgc tgtttacgct
    73621 gatgaaaaag ctgcgcgtgc agctggtgag gagcgatcaa aacgtgccct cctcaaacaa
    73681 cgtcaaaata cacagcgtgt tagccttgat aatctctttg atacccttaa agcaggagaa
    73741 atcaagactg ttaatgtcat catcaaagca gacgttcaag gttctgttga agcccttgca
    73801 gcatctcttg taaagattga agtggaaggt gtccgtgtca atgtcgttca ctctgctgta
    73861 ggtgctatca atgagtctga tgtgaccctt gctgaagcat caaatgctgt tattatcggt
    73921 ttcaacgttc gccctacccc acaagctcgt caacaagcag atacggatga tgtggaaatt
    73981 cgtcttcata gcattatcta taaagtcatt gaagaagtag aagaagccat gaaagggaaa
    74041 cttgaccctg tttaccaaga aaaaattctt ggtgaagcca ttattcgtga aaccttcaaa
    74101 gtttctaaag tgggaacaat tggtggcttt atggtaatca acggtaaggt gacacgtgat
    74161 tcgagtgttc gtgttatccg tgatagtgtc gttatttttg atggtaaact ggccagcttg
    74221 aaacattaca aagatgacgt taaagaagtc ggtaatgctc aagaaggtgg gttgatgatt
    74281 gaaaacttta acgatcttaa agttgacgat actattgaag cctatattat ggaagaaatt
    74341 gtgagaaaat aggggctaag aaggtctgag atgtgacgtt tttcctctgt cggaaataat
    74401 gacttgttct accagatgag ctacttgggg cattccttcc agtctcgtca tggaattctc
    74461 agattccctt tagttgtcgt tatcaaaaca ggtagcaatg tcataagctg ggctttcagc
    74521 ctagcttttg ttttaaaaat gtggaaagga aatggctatg gctaatcatc gtattgaccg
    74581 cgttgggatg gaaatcaagc gcgaagtaaa tgatattctt cagaaaaaag tgcgtgatcc
    74641 acgcgttcag ggagtaacga ttacagaagt gcaaatgcag ggtgacttgt cacttgctaa
    74701 agtttattac actattatga gtgacttggc atctgataat caaaaagcac aaacagggct
    74761 tgaaaaagcg actggaacga ttaaacgtga acttggcaaa caattgacca tgtacaaaat
    74821 tccagatttg gtgtttgaaa aagacaattc catcgcctat ggtaataaaa tagatcaact
    74881 gttacgtgag cttgataaaa aagattaaat taaaaacctt taaggtatct tattagagtc
    74941 accttaaggg ttttttgttt ttagtaagct ttttgaatcg tatcaatgat ttgatattgt
    75001 gcagtcttgg cttcttcaat tggatagatt gggtaaacat gattcatttt atcagcgacg
    75061 ataagttcat gttgatgacc tttggcagtt aatatatcat caaatttctt aacatctggg
    75121 tagaaaattt catgtgtacc aacaaacata gtaataggtg ctagccctga tacatctcca
    75181 ttaattggac taacatatgg atgtttggtg ttgtttgggt cacggcgtgc ccaaatttcc
    75241 ccaactttac gtaggcccca agctgaaaga atgggatcat tactttcata gctctcaata
    75301 tctggatttg acatggttac atctaaccat ggtgataaga gaacaatgtc ttttggttgt
    75361 tttaaacttt cattttttag ggctaatgct aaaccaagcg ctagtccacc acctgctgaa
    75421 tcacccataa tagtgatatt ttgtgcagtt gtttctttaa gagtatctct gtaaaggtca
    75481 accaattttg gcatagcatc actgtaagag taagtaggtg cttttggata gattggtaag
    75541 ataatcttag catcaagact agttgataag gtttttagca tgtcgatatg ataagtagtt
    75601 gggttgttta ggtaagagcc cccatgtaaa tagaaaatga ccttttgatt ggctttattg
    75661 ctggggttct cattccaagt atgaacttgc atattatcaa cagttttagt gactaatgtg
    75721 ccatttacct gttcttcttt aagatcaaag cctttatttg aagcttctcc agcagagttg
    75781 acatattgat caaacttagt ttcgtcttgc tctgaataat agcctttgtc tccttttaac
    75841 caaaagtatt tctctataaa ccagcttttc caagatctag aactggcatc aacttgagtg
    75901 ctagctccta ttgctagagt aagtcctagt aaaataagag tgcttttaag tgtc
//
LOCUS       DAUGFP010000004        69266 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.004, whole genome shotgun sequence.
ACCESSION   DAUGFP010000004 DAUGFP010000000
VERSION     DAUGFP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 69266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69266
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.004"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            142..873
                     /gene="aphA"
                     /locus_tag="VH663_000228"
     CDS             142..873
                     /gene="aphA"
                     /locus_tag="VH663_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEQ8173461.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINATKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            1051..2583
                     /locus_tag="VH663_000229"
     CDS             1051..2583
                     /locus_tag="VH663_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEQ8173462.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVVVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGSWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            2746..3360
                     /locus_tag="VH663_000230"
     CDS             2746..3360
                     /locus_tag="VH663_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEQ8173463.1"
                     /translation="MLEIVSEELRRYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQAYI"
     gene            3519..4667
                     /locus_tag="VH663_000231"
     CDS             3519..4667
                     /locus_tag="VH663_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ8173464.1"
                     /translation="MIIKGVTMKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYK
                     TSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGICLLLAYLTFFILITW
                     IISIVIPDLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYS
                     QQLLKQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTG
                     KYAKRIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTA
                     LIPVIGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAM
                     WVLMAITIGASLKGIVGMIIAVPLAATLYQVIKDNIQKRQAIQKKQVS"
     gene            4716..5396
                     /gene="radC"
                     /locus_tag="VH663_000232"
     CDS             4716..5396
                     /gene="radC"
                     /locus_tag="VH663_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEQ8173465.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIIEEKTTFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
     gene            complement(5398..6093)
                     /locus_tag="VH663_000233"
     CDS             complement(5398..6093)
                     /locus_tag="VH663_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEQ8173466.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTVAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(6103..6747)
                     /locus_tag="VH663_000234"
     CDS             complement(6103..6747)
                     /locus_tag="VH663_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEQ8173467.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
     gene            complement(6998..7345)
                     /locus_tag="VH663_000235"
     CDS             complement(6998..7345)
                     /locus_tag="VH663_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEQ8173468.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLETIPNSGDGFPLKITINKELTGFKLSITDQSGLRLVNIFKTKDNQILQDKFYFLMD
                     SLVEREIFSKTVV"
     gene            complement(7335..8462)
                     /locus_tag="VH663_000236"
     CDS             complement(7335..8462)
                     /locus_tag="VH663_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ8173469.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     NTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            complement(8459..9439)
                     /locus_tag="VH663_000237"
     CDS             complement(8459..9439)
                     /locus_tag="VH663_000237"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ8173470.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAAEVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            complement(9579..10157)
                     /locus_tag="VH663_000238"
     CDS             complement(9579..10157)
                     /locus_tag="VH663_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEQ8173471.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLSQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFVNSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHITFKYAKSKVARCINTLKKFNDK"
     gene            10245..10916
                     /locus_tag="VH663_000239"
     CDS             10245..10916
                     /locus_tag="VH663_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ8173472.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            10891..11727
                     /locus_tag="VH663_000240"
     CDS             10891..11727
                     /locus_tag="VH663_000240"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEQ8173473.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEI
                     DS"
     gene            11724..12629
                     /locus_tag="VH663_000241"
     CDS             11724..12629
                     /locus_tag="VH663_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ8173474.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            12633..13628
                     /gene="pta"
                     /locus_tag="VH663_000242"
     CDS             12633..13628
                     /gene="pta"
                     /locus_tag="VH663_000242"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEQ8173475.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKETFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFIFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            13755..14510
                     /locus_tag="VH663_000243"
     CDS             13755..14510
                     /locus_tag="VH663_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEQ8173476.1"
                     /translation="MGNIALVTGASAGFGRAIVEHLIADGIKVIGASRRLEKLKVLET
                     SFGSDNFYPLQMDVTDTKAIDDALTSLPNQWQDITILVNNAGLALGFDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDDGIIINLGSTAGTIPYPGANIYGASKAFVKQF
                     SLNLRADLAGSHIRVTNIEPGLCEGTEFSKVRFNGDEERVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVSRD"
     gene            complement(14704..15393)
                     /locus_tag="VH663_000244"
     CDS             complement(14704..15393)
                     /locus_tag="VH663_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEQ8173477.1"
                     /translation="MKNAISILALLTPENQLEETFIRELSHHFSHLFEVTILTSKANI
                     QSNQLSTFQGIFSFHEHDIDFPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTGF
                     YQKFDGHFLQYLPLQARLSDANGNIIVDNHAFNGSFLPTTDKEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLNQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            15813..16541
                     /locus_tag="VH663_000245"
     CDS             15813..16541
                     /locus_tag="VH663_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173478.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNAPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPEM
                     KQWAPEEVRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVANLFGGDEHA"
     gene            16534..18060
                     /locus_tag="VH663_000246"
     CDS             16534..18060
                     /locus_tag="VH663_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEQ8173479.1"
                     /translation="MPSLFITFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGTAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITFL
                     VLLASYTPMVLSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDIYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAEEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKASLLTKHPELKGILNQLAGKITEKEMQDMNYKVSVKGADANKVARDYLLKAGLIQK
                     "
     gene            18338..19321
                     /gene="guaC"
                     /locus_tag="VH663_000247"
     CDS             18338..19321
                     /gene="guaC"
                     /locus_tag="VH663_000247"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEQ8173480.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAGQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKAYEY
                     EFVTSLKEDAPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVNGETFKEYYGSASAYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     regulatory      19444..19539
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19626..20207
                     /locus_tag="VH663_000248"
     CDS             19626..20207
                     /locus_tag="VH663_000248"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEQ8173481.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     gene            20207..21490
                     /locus_tag="VH663_000249"
     CDS             20207..21490
                     /locus_tag="VH663_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEQ8173482.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSGAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIH
                     VPTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIVAVSISAGLGFNGTNLFAS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(21554..22492)
                     /locus_tag="VH663_000250"
     CDS             complement(21554..22492)
                     /locus_tag="VH663_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEQ8173483.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQAAGVKPVSVHSDLFNLVQIGKAHSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVRVHGPNPKSPDKIFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTAKGKQYHHILDRQTGYPIETDMLSLTIMAPSSFYCDIWTTRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(22545..22730)
                     /locus_tag="VH663_000251"
     CDS             complement(22545..22730)
                     /locus_tag="VH663_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEQ8173484.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            22868..23437
                     /locus_tag="VH663_000252"
     CDS             22868..23437
                     /locus_tag="VH663_000252"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEQ8173485.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            23472..24551
                     /gene="prfA"
                     /locus_tag="VH663_000253"
     CDS             23472..24551
                     /gene="prfA"
                     /locus_tag="VH663_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEQ8173486.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDLNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            24551..25390
                     /gene="prmC"
                     /locus_tag="VH663_000254"
     CDS             24551..25390
                     /gene="prmC"
                     /locus_tag="VH663_000254"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEQ8173487.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLAYVFSEIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            25374..25964
                     /locus_tag="VH663_000255"
     CDS             25374..25964
                     /locus_tag="VH663_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEQ8173488.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITAALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            25982..26434
                     /locus_tag="VH663_000256"
     CDS             25982..26434
                     /locus_tag="VH663_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8173489.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            26424..27680
                     /locus_tag="VH663_000257"
     CDS             26424..27680
                     /locus_tag="VH663_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEQ8173490.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAITDHVGAYLMVDMAHIAGLVAAGVHPSPV
                     PYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALVPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIARLIIKALVN
                     HNQTVILEEVRQEVRQLTDAFPLYKK"
     gene            27687..28664
                     /locus_tag="VH663_000258"
     CDS             27687..28664
                     /locus_tag="VH663_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEQ8173491.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVGQVKEVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            28665..29264
                     /locus_tag="VH663_000259"
     CDS             28665..29264
                     /locus_tag="VH663_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEQ8173492.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIKHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            29274..30998
                     /locus_tag="VH663_000260"
     CDS             29274..30998
                     /locus_tag="VH663_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173493.1"
                     /translation="MKRLRPYLKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLISVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHIGYASLLSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLDPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KEQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEAPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPINDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKVLKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGEQK"
     gene            30995..32722
                     /locus_tag="VH663_000261"
     CDS             30995..32722
                     /locus_tag="VH663_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173494.1"
                     /translation="MIKTDHHLLKRVLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVIIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQMVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLTPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEILRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DVGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRDEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            32963..34333
                     /locus_tag="VH663_000262"
     CDS             32963..34333
                     /locus_tag="VH663_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEQ8173495.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDAKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIELFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDMSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(34492..35475)
                     /locus_tag="VH663_000263"
     CDS             complement(34492..35475)
                     /locus_tag="VH663_000263"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEQ8173496.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIIDKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            35666..38152
                     /gene="gyrA"
                     /locus_tag="VH663_000264"
     CDS             35666..38152
                     /gene="gyrA"
                     /locus_tag="VH663_000264"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEQ8173497.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDILAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELISTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIDEINTDRENSISKNKDN"
     gene            38172..38921
                     /locus_tag="VH663_000265"
     CDS             38172..38921
                     /locus_tag="VH663_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEQ8173498.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIAVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQVST"
     gene            39004..39417
                     /locus_tag="VH663_000266"
     CDS             39004..39417
                     /locus_tag="VH663_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ8173499.1"
                     /translation="MKLNAIHHVAIIVSDYHLSKDFYVNKLGFEIIRENYRPDKHDYK
                     LDLSCGRIELEIFGKVTSDPYYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
     gene            40031..40468
                     /locus_tag="VH663_000267"
     CDS             40031..40468
                     /locus_tag="VH663_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173500.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKNSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEEKWQDTKDAVEVYGGIAAE
                     TVEESLGQAKEKVEGIGEATSQTIQSKMEKLTSDKTDADDDEK"
     gene            complement(40518..41429)
                     /locus_tag="VH663_000268"
     CDS             complement(40518..41429)
                     /locus_tag="VH663_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ8173501.1"
                     /translation="MKTVRIFYNPNSGKKEDQLAGQVKDYFCQHGFSEDSVEVITPKG
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIKQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGDFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(41546..42196)
                     /locus_tag="VH663_000269"
     CDS             complement(41546..42196)
                     /locus_tag="VH663_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEQ8173502.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(42193..42633)
                     /locus_tag="VH663_000270"
     CDS             complement(42193..42633)
                     /locus_tag="VH663_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEQ8173503.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQAGSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLSQEVTQ"
     gene            42852..43700
                     /gene="ylqF"
                     /locus_tag="VH663_000271"
     CDS             42852..43700
                     /gene="ylqF"
                     /locus_tag="VH663_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEQ8173504.1"
                     /translation="MAMIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFREYY
                     PNRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GNMDAD"
     gene            43690..44481
                     /locus_tag="VH663_000272"
     CDS             43690..44481
                     /locus_tag="VH663_000272"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEQ8173505.1"
                     /translation="MPTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKA
                     IQAELAEEERLEAMLSYEKALYKKGYQAIAGIDEVGRGPLAGPVVAACVILPKYCKIK
                     GLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            44546..45382
                     /gene="dprA"
                     /locus_tag="VH663_000273"
     CDS             44546..45382
                     /gene="dprA"
                     /locus_tag="VH663_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEQ8173506.1"
                     /translation="MNHFELYKLKKAGLTNKNILNILDYQKQQEKSLSLRDMAVVSGC
                     KHPSHFIEAYKQLDIQKLKIEFKQFPSISILDKHYPMALKEIYNPPVLLFFQGNLDLL
                     EKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
                     AIIGTGLDRFYPKENRELQTFLGKNHLVITEYGPGEEALSYHFPERNRIIAGLSRGIL
                     VVEAKNRSGSLITCQIGIEEGRDIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILS
                     EY"
     gene            45489..47618
                     /gene="topA"
                     /locus_tag="VH663_000274"
     CDS             45489..47618
                     /gene="topA"
                     /locus_tag="VH663_000274"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEQ8173507.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMAEGETVKKISTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(47693..48577)
                     /locus_tag="VH663_000275"
                     /pseudo
     CDS             complement(47693..48577)
                     /locus_tag="VH663_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            48590..49135
                     /locus_tag="VH663_000276"
                     /pseudo
     CDS             48590..49135
                     /locus_tag="VH663_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            49150..50142
                     /locus_tag="VH663_000277"
     CDS             49150..50142
                     /locus_tag="VH663_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEQ8173508.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESPLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWGLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGRKVVGYDIYQSDAAKAVLDYKESVEEAIKDADLVSLHMPPTAENTHLFNS
                     DLFKSFKKGAILMNMARGAVIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            50247..50912
                     /locus_tag="VH663_000278"
     CDS             50247..50912
                     /locus_tag="VH663_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEQ8173509.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLALRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     ADD"
     gene            50905..51620
                     /locus_tag="VH663_000279"
                     /pseudo
     CDS             50905..51620
                     /locus_tag="VH663_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
     gene            51766..53112
                     /gene="trmFO"
                     /locus_tag="VH663_000280"
     CDS             51766..53112
                     /gene="trmFO"
                     /locus_tag="VH663_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEQ8173510.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAKSVTAELENHPLIEVIRDEITEIPNDAITVIATGPLTSDALAEKIHAVNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPL
                     NAFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRSNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            53265..54671
                     /locus_tag="VH663_000281"
     CDS             53265..54671
                     /locus_tag="VH663_000281"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEQ8173511.1"
                     /translation="MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTIL
                     DKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHY
                     ADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
                     TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSG
                     ISQMTYLAAVEAGADRIDTALSPFSEGTSQPATESMYLALKEAGYDITLDETLLEQAA
                     NHLRQARQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLA
                     EVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPV
                     NEDLKRSQIGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFL
                     TTKYQTDDVIKVTAFIKA"
     gene            54714..55028
                     /locus_tag="VH663_000282"
     CDS             54714..55028
                     /locus_tag="VH663_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173512.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKKTL
                     SQQASSIPQEQEADKLSGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVIAVER
                     KS"
     gene            55028..55378
                     /locus_tag="VH663_000283"
     CDS             55028..55378
                     /locus_tag="VH663_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEQ8173513.1"
                     /translation="MKRYEVTVNGQVYEVSLRELADGETVEVSQPAAPATEKEMNANA
                     AGGGIQVKTPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            55388..56518
                     /locus_tag="VH663_000284"
     CDS             55388..56518
                     /locus_tag="VH663_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEQ8173514.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLACFFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAGLMDHPANGNPGGLLYYLYKGTSLGIYPPLIFLCLGASTDFG
                     PLIANPKTILLGGAAQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLA
                     PDLLSTIALAAYSYMALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVT
                     IFVSLLVPSATTLVGCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANG
                     DLFLSATTLKIIALGLIAFAAGTAGGVLMGKVMYYLSGNKVNPMIGAAGVSAVPMAAR
                     VVQKVGQEEDPSNFLLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(56719..57603)
                     /gene="citG"
                     /locus_tag="VH663_000285"
     CDS             complement(56719..57603)
                     /gene="citG"
                     /locus_tag="VH663_000285"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEQ8173515.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFM
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGCFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(57596..58291)
                     /locus_tag="VH663_000286"
     CDS             complement(57596..58291)
                     /locus_tag="VH663_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8173516.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKKTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQAYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(58449..59852)
                     /locus_tag="VH663_000287"
     CDS             complement(58449..59852)
                     /locus_tag="VH663_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEQ8173517.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYIPQ"
     gene            60232..60555
                     /locus_tag="VH663_000288"
     CDS             60232..60555
                     /locus_tag="VH663_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173518.1"
                     /translation="MQFLQKWFKHSKKEKLKESSPLSTEIEPSENWEKIPAYIPADKS
                     DYKKVTLITSAIAAGDRPNSQFKVKRILKRNSEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
     gene            60612..61007
                     /locus_tag="VH663_000289"
     CDS             60612..61007
                     /locus_tag="VH663_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEQ8173519.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVPVPTEA
                     SPQVGEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VASSAGTITAIHVGPGQVVNPGDGLITIG"
     gene            61052..62173
                     /locus_tag="VH663_000290"
     CDS             61052..62173
                     /locus_tag="VH663_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEQ8173520.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIETELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRMTYKAENVSQMTKILFPIIITLVA
                     GFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQNELVNIISILLGLTISIKMQADLF
                     LNVQTLLIIVFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQ
                     KMATDEDPQNFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            62361..62669
                     /gene="citD"
                     /locus_tag="VH663_000291"
     CDS             62361..62669
                     /gene="citD"
                     /locus_tag="VH663_000291"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEQ8173521.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEQTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGIDQYDWKEIDSWNV
                     "
     gene            62657..63544
                     /gene="citE"
                     /locus_tag="VH663_000292"
     CDS             62657..63544
                     /gene="citE"
                     /locus_tag="VH663_000292"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEQ8173522.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSVMFDLEDSVSLKEKD
                     TSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDIV
                     DVEAVIERVERENGIEVGRTRMMAAIESAEGVLNARDISKASKRLIGIALGAEDYVTN
                     MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDG
                     KSVINPRQIPLVNEIYTPTKKEIDHAKQVIWAIREAESKGSGVISLNGKMVDKPIVER
                     AERVIALATAAGVLSEEDI"
     gene            63547..65079
                     /gene="citF"
                     /locus_tag="VH663_000293"
     CDS             63547..65079
                     /gene="citF"
                     /locus_tag="VH663_000293"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEQ8173523.1"
                     /translation="MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPG
                     DSKLLGSIREAIEKTGLTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSI
                     ANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
                     HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPI
                     SIPQTDVDYVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSF
                     QTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEGLVEKILDVQDFDHP
                     SAVSLGKHAEHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVI
                     RGASGGHCDTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINP
                     NRQDLADHFKSLNVPQFSIEELKEKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVY
                     NV"
     gene            65072..65650
                     /gene="citX"
                     /locus_tag="VH663_000294"
     CDS             65072..65650
                     /gene="citX"
                     /locus_tag="VH663_000294"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEQ8173524.1"
                     /translation="MCKDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEAFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            65665..67059
                     /locus_tag="VH663_000295"
     CDS             65665..67059
                     /locus_tag="VH663_000295"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEQ8173525.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFI
                     QKSIENGIDIVRIFDALNDSRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLADKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEDIKEKII
                     GDEAVITCRPADLIEPQMIYLRDEIAPYARSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(67169..68221)
                     /gene="citC"
                     /locus_tag="VH663_000296"
     CDS             complement(67169..68221)
                     /gene="citC"
                     /locus_tag="VH663_000296"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEQ8173526.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGEGLLNRIVSHLIDEEYTLGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAVVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDRSLIPFSVRKRLIQEGLAHLDNVIYHETGPYLISQ
                     ATFPAYFQKEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDNDWELLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(68310..68792)
                     /locus_tag="VH663_000297"
     CDS             complement(68310..68792)
                     /locus_tag="VH663_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEQ8173527.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
BASE COUNT        21607 a        12348 c        14073 g        21238 t
ORIGIN      
        1 tcgattatga taatttttta gatcatcttt gttgaataac ttcatcctta ttcagaactt
       61 aaagggcact attataagaa tttgaaaccg ctttacttaa accgtgtatt tttgtacaat
      121 aatgattaag gaggttttta gatgaaaaaa gaatttacca gtatcctgtt tacagttagt
      181 ttttgtggaa tcatagcatt gcctgtcgaa gctagtggcc ctaaagttcc ttatacacaa
      241 gaaggtataa cggccatttc aaatcaggca acagtaaaat tgatttctat tgctgatatc
      301 gcgagcagtt tagaaggaca aaaacctata acggttagtt ttgatatcga tgatactttg
      361 ttatttacta gccaatattt tcagtacggc aaagaatata taactccagg ctcatttgat
      421 tttttgcata agcaaaaatt ttgggactta gtcgctaaac ggggagatca ggattctatt
      481 ccaaaagagt atgcaaagca actaatcgca atgcatcaaa aacgaggaga taaaattgtc
      541 tttattactg gccgaactcg aggatcgatg tataaaaaag gtgagattga taaaacagct
      601 aagtcactgg ctaaagattt taagttagac aagccaatag ctatcaatta tacaggagat
      661 aaagctgtga agccgtatca gtatgataaa acatattata taaagaaaaa cggctcacaa
      721 attcattacg gagatagtga tgaagatata aatgctacca aagaggcagg tgcaagacct
      781 attcgcattt taagagctcc taattcaacc aatcttcctt tgccaaaagc tggaggatac
      841 ggagaagagg ttttagaaaa ttcggcttat tagtcaaata tttgtgtaag aaaggaagta
      901 acagtaattt ctttcatcac cccactgagt aaccaatagc ccagtgggtt ttttgggtta
      961 cagaaatcag ttctaaaaaa gaatcactgg taacttaagg ttgtctttga tacaatggta
     1021 acagtgtatt taaaagaaag agaggacaac atggaaaacc ataaaaatga attcaccttt
     1081 tccaacaaat caattattgc ctatgtttgg cgtggtgttg tggttggtat tattgctggt
     1141 gttattgtta gtttattccg gttgttaatt gaagtgacag ctgactgggt gattgaatgg
     1201 taccgatacg ctcatatcaa ttcgctgttg ctacttccta tactaagtgt cagtttgtta
     1261 gctgttcttt ttgtaggttt tttagtaaag tctgattctg atataaaagg atcagggatc
     1321 ccccatgtcg aaggagaact aaaggggcta atgtccccag attggtggag tgtcctttgg
     1381 aaaaaatttt taggaggaat catggctatt tctatgggat ttatgctcgg tcgtgagggt
     1441 ccttcgattc agttaggtgc tatgtcagcc aaaggattag ctaagttttt aaaatcgagt
     1501 cgtttggaaa agcgagtgct aattgccagc ggagcagcag cagggctatc agcagctttt
     1561 aatgctccaa tagcaggcct cttgtttgtt gttgaagaaa tttaccatca tttttcgcgt
     1621 ttaatttgga ttacagcgct agtggctagc ttagtggcta attttatttc tctgaatatt
     1681 tttggattga aaccagtctt agcaatgtca gaggcgatgc cttttttagg actcaatcaa
     1741 tattggttgt tgcttttact tggattattt ctaggctgct taggctattt atatgagata
     1801 gttatcctta attttaataa actttatgtt attttaggga gttggttaca tctgccagct
     1861 tatttttacg gtattattat ggtttttcta attttaccga ttggctatta tttaccacag
     1921 ttacttggag gaggacatgg tttaatactt tctttatcca atcaacaatt gcctttgatg
     1981 actatcttct tttattttat tattcgattt attgttagta tgtttagtta tggaagtggc
     2041 ttacctggag gtattttttt acccatttta actttaggtg ctttggcagg cctattattt
     2101 gggcaaatag cttctcaatt aggtttattg aaccaatctt ttctttctct ttttttgata
     2161 ttagggatgg cagggtattt tgcggcaatt tcaaaagcac cactgacagg catgattttg
     2221 gtaactgaaa tggtaggtga tttgaaaccc ttaatggcca tagctgtagt tacttttgtc
     2281 tcctatttag tgatggattt gttaaatgga cagccaattt acgaagctat gctagataag
     2341 atggcaatga agcatccaac taatcttgtt gaaccaacat taattgaatt gactgttggt
     2401 gataagattg cagggaaata tgttaaagag ttaaagcttc cagaaaatgt cttaattaca
     2461 acccaaattc accatcaaaa atctcaagtt gtgtcaggta acacgcgcct tttatcagga
     2521 gcaactatct ttttggtcgt taatgaagct gatacaggtt ttgtccgtga ggtattgatg
     2581 taggtattaa tttatcacaa ttcggaaagt tcgagcttgt cttttagact aagtctttga
     2641 acgttttact gctagagttt catctgaacc aaagtgaaac gcttttgatg aagtcaagta
     2701 ggctttttgt tatactaaca ataaagataa gctaaggagg ctgagatgtt agaaattgtt
     2761 tcagaagagc tacgtcgcta tcaagaacaa aaattgattg agtaccgtaa caaaaatcag
     2821 ttagccccaa aaggaggcat tgtatttgca ggtgattctc tcattgaatt ttttccttta
     2881 aaaaaagctt ttggttcatg tttgcctatt attaatcgag gtattgctgg gatagatagt
     2941 cagtggttat tgcgccattt ttctgtccaa attactgatt tagagcctaa acatattttt
     3001 ttattaattg gttgtaatga tatcgggctt ggatatgata aatgtcatat tgtcaagact
     3061 attgtagagc ttattagcca gattcgtagt cattgtgtct acagtcagat ttatctctta
     3121 tctttattac cagtttccaa caatccccga tatcagaaga ctgtcaaaat aagaacaaat
     3181 gctatgattg atgctattaa taaagattta gcaatgattc caactataga atttattaat
     3241 cttaatactt gtcttaaaga cgaaaaaggt ggtttatcag atgagaatac tctagatggt
     3301 ttacatctta attttccagc ttatgctaaa ctagctgaaa ttattcaagc ttatatctag
     3361 caaataaatg tactaatagc gatgaggcaa gaagtcagat agcagatggt tcatcaatca
     3421 attaagaaaa aagttatcaa gataccatta aaaatagttg ctcaaagttc tttcaaatca
     3481 aaagattatg atatcgctat gttgagtttt agtgtataat gataattaaa ggagttacca
     3541 tgaaatttga aaaaaaacaa gtcttctatt tagtccttac gtttatctta tgctatggga
     3601 tcctagcaaa ctggcgaaat ggaacggcta tcgtcacgac tatttacaaa acaagccttc
     3661 ctttttttta tggagcagca ggtgcctata ttgttaatat tgtaatgagc gcttacgaaa
     3721 aggtatatgt ttatatcttt aaagactggt cacatgtctt aaaagtaaaa cggggtattt
     3781 gtttactgtt agcgtatttg acatttttta ttttaattac ttggattatt tccattgtca
     3841 ttccagactt aattacaagt attagtactc taacaaaatt tgatactata acgatacaag
     3901 aagttgttaa caatcttgag cacaataaac tattagcacg tactattcag tatattggtg
     3961 gcgatggcaa acttacagag acgattgcta actatagtca acagttatta aagcaatttc
     4021 ttactgtttt gacaaatatt ttaacatctg tcacagttat tgcttctgca attattaatc
     4081 tttttattag ttttgttttt tcgttatacg ttttagcaag taaagaagat ctttgtcgcc
     4141 agggaaatac tttagtagat acttatactg gtaagtatgc taaacgcatt cattacttgt
     4201 tagagttgtt gcatcagcgt tttcacggat tttttgttag tcagacctta gaagctatga
     4261 ttttaggttc gctgactgct agtggcatgt ttatattaag attaccattt gcagggacta
     4321 ttggcgtttt agtagctttt acagcattga ttccagttat aggtgcttca attggagcag
     4381 ctattggatt tattttaatt atgactcagt ccatgtcaca ggccattatt tttatcattt
     4441 ttttaattat tttgcagcag attgaaggca attttattta tccgaaagta gttggtggat
     4501 cgattggatt accggctatg tgggtattaa tggcaattac aataggcgct tctttaaagg
     4561 gaatagttgg tatgattatt gcagttcctt tagcagcgac actttatcaa gtgattaaag
     4621 ataatattca aaaaagacaa gctattcaaa aaaaacaagt ttcctaaaag gaacttgttt
     4681 ttttgtcgaa taaaaagatg gaggtaatat gataaatgta ttctataaaa tgtgatgata
     4741 ataaagccat gccaagagaa cgtttgatgc gactaggagc agagtctcta agtaatcaag
     4801 aattattagc aattttatta cgaacaggta ataaagaaaa gcatgtctta gagctgtcat
     4861 cctatctttt atcgcattta gacagtctgg cagattttaa aaagatgtct ttgcaagaat
     4921 tacaacattt ggcaggtata ggaaaagtta aagcgattga aattaaagct atgattgagt
     4981 tggtttcccg aattttagcg acagataaga cattaactga tagcgtatta accagtgttc
     5041 aggtcgctga aaaaatgatg gcagctttag gagataaaaa acaagagcat ttagtcgtat
     5101 tgtatttaga taatcaaaat cgtattattg aagaaaaaac tacttttatt gggactgtcc
     5161 gacgttcact tgcagaacca agagaaattt tatactatgc ctgtaaaaat atggcgacta
     5221 gtctcattgt tattcataat catccttcag gaaatattga acctagttct aacgattatt
     5281 gctttactga aaaaataaaa cgatcatgtg aagatttagg cattatctgt ctagatcaca
     5341 ttatcgttag ctataaagat tattatagtt ttcgagaaaa atcaaccctt ttttaaatca
     5401 gaagtcattg acgaataagc ggaacaattg tagatcttca tctcgaacac cttggagaag
     5461 ttcaggatgc cactgaacac ctaaaaaagg aatggcatcg ttggttgata tgactgcttc
     5521 gatggtacca tcacgtggat ctctagcgat tacttttaaa tccttagcaa cagtttttaa
     5581 actttggcga tgaaaagaat taattaatgt cttatgaccg taaataggat ataagataga
     5641 gtctggctca ataatcattt cgtgtgataa gaaatcagaa ggcgcttctt gccaatgtga
     5701 gtcaatgtgt tggttaagat ttcctcctaa ggcaacattc attaactgtg taccacgaca
     5761 aatccctaag attggttttt ttaatgttat agcctccttg atgatagcta gctcaaatgt
     5821 gtctcgctct ggtgaaaaat catcatcaaa agcagccttc tcttcttgat aatattttgg
     5881 gtcaacgttt tgcccaccaa ttaaaatgat tttatcaacc atagatacat aagttttagc
     5941 ggcggcttca tcaccaatgg gtaaaagaag cggcagcccc ccagattgag tgactgcctg
     6001 tacaaatcca gtaggtgcat atgaccatgg gagattatcc aaagccatat ttagtctttg
     6061 atttgcagtt attccaataa tcggtttggt cattatacct ccttaatcta gatgattttg
     6121 attcataaaa tagagtagtg tctggagctc actggtcaaa tcgacagatt gaacaataac
     6181 tcctttagga acttgtaggt gaacaggagc aaaactcaag attcctttga ttcctgcttc
     6241 aataagttga tcagtaactt cttgagcatg aatgctggga accgttaaga tagccgtttc
     6301 aatgtcagtg ttcgcaatcc gttctttgac agatgagata ccatggactg gaatattatc
     6361 agctgtttta gtaccaacaa gagcgttatc atcggtatca aaacccatgg caatttgcat
     6421 cttattacga tcatggaagc ggtaatgtaa tagagcacgc cctatattac cgcatccaac
     6481 taagataacg tttgttgtag agtgatcatt aagcaagtca gcaaaaaaat tcatcaattt
     6541 ggtaacatca tagccaaacc cacgacgccc caattctcca aaataagaaa agtcacggcg
     6601 tacagtagca gagtcaattc ccattgcatc tgcaatttgt ttagaactgg ctttttcaac
     6661 ttggtcagca tggaatcgtt taaaaatacg gtaataaagt gataagcgct ttgcagtcgc
     6721 ttttggaata gatttgtcaa taaccactat tttactcctt gtagatttct cgttaaatat
     6781 gtctgtcttt attgtaacaa aaagattgtg aaaaatgcaa cattttgatt gtttttgtat
     6841 gagttaaaaa agctaaaatt agttttagca ctatataata gtattcatta ggaaagtaag
     6901 ttatgacagt caaaatgaac aacatgaatt aatagcaata gaagcgaaat catttattga
     6961 cattgtgata tgactaaaaa acaaaattta aagttgatta gacgactgtt ttgctaaaaa
     7021 tttcacgttc tacaaggctg tccatcaaga aataaaattt atcttgaagg atttgattat
     7081 cttttgtttt aaaaatatta actaatctaa gaccagattg atcggtgata gataatttaa
     7141 aacctgtgag ttctttatta atagtgattt ttaaagggaa accatcccct gaattaggta
     7201 ttgtttcgag taaacgtatt aattggtagt gccctacttt agtttgacta aatgtagtat
     7261 ctcttagggt atatttttta atgtttggac tgattgtata aaggtattta cagtctttaa
     7321 gcttaatttc tttttcaaaa gccatctgtt cctcctaaaa tattttttaa tgtttgagct
     7381 aattgattaa catcttcaat gctattttga tctgaaaaac tgatacgaat tgattctttt
     7441 aaacgagaag agtcatcccc atagtacgca gctaaaacat gacttggatt aacagctcca
     7501 gcagtacaag cagaaccagt tgatactgca atgcctgcta agtcaagttg tgttagtaaa
     7561 atagtatttt ggtagcctaa aaatccaata ttgagaacat gaggaagata atgagtacct
     7621 tgattaatat agtagggtag tccttctagg aggctaatca agtgatgacg taaactaata
     7681 atgtgatcag ttgattggtc aagacaagtc atagcatcag tcaaagcttg tgccatacca
     7741 ataataccta acatattttc agtacttgcg cgtcgctttc cttcttgatc acccccgtga
     7801 agaagaggat cgattggttg tccgttactg tataaaaagc cgcatccttt aggaccatga
     7861 aatttgtgag cagaagcaga taaaaagtca atcccaagtt cgctaggaat aatcttgagt
     7921 ttgccaacag cttgaacggc atcaacatga aaagcagctt gatggtcttt gagcagattc
     7981 ccaatgtcct taataggtaa caggtcacct gtttcgttat tggcatacat tatgcttacc
     8041 aaaatggtgt catctctcaa ggcttgtttt aagtcagata aatttatttg tccattttga
     8101 caaggcaaat aagtgacctc aaaaccaaat cgctcttcga gataggccat ggtattgagc
     8161 acagagtggt gctcaatagt agttgtgatg agatgtttgc cctttgcttg atgagctaaa
     8221 gcatagcctt ttatggccat attattgctt tcagttccac cggacgttac tataatttgc
     8281 tgttcacttg cccccaagtt tctagcgata gcttgtcggc attcacgaag gattttattg
     8341 gctcggcgcc cgtagaaatg aatactagag gggttaccaa agttatcttg catagctgct
     8401 gtcatcgccc taatcacatt aggactaagt ggtgtggtgg cggcgttatc aaagtaagtc
     8461 atgcgttatt tttgccttta ggttgataag aaaaaagtgg acttaagggt cttctttcat
     8521 ggatacgaat aatagcttct gcgattaaat cactggcgct aagataagta acgttttctg
     8581 gcacacgatt tttagtttta acagaatctg taacgataat ttctttgatt ggagctgttt
     8641 ctaatacctc agcagctcca ccagcaaata agccatggct agcgaccgcg taagtatcag
     8701 tagcgcctga gcgttcaaga attttagcag cttctgcaaa ggttttccca gtatttaaaa
     8761 tatcatcaat taaaatggct tttttaccag aaacatcacc aataatataa ccttgttcac
     8821 gttcagaatc atcttgtgca tagtcgataa tagcaatcgg ggaatcaaga tattcagcca
     8881 agcttctagc acgtttaatt ccagaatttt tagggctaac gacaacaaca tcagaacctg
     8941 ataagcctaa tttactgtaa cgttctgcaa aaagagggac tgtaaagagg ttatccactg
     9001 gaatatcaaa aaaaccttgt acctgaacag catgtaagtc aagcgtcact acacgatcaa
     9061 taccagcttt agtcaacata ttagctacta gtttagcagt aatcggttcg cggggcttag
     9121 cgacacggtc ttgacgagag taaccaaagt atggcaatac aatattgacg gtattagcac
     9181 ttgcacgttt acaagcatca atcatgatga gtaattccca aagattatca ttgacaggaa
     9241 aactagtgga ttgaataata tagatatcat ctccacgcac tgtttcttca atattgatca
     9301 taatttctcc atcggaaaat tgacgtgaag acatttttcc aagagggatt ccagcagctt
     9361 tagcaatttt ttcagcaatt ggaagatttg atgtgagtga gaacagtttg atttgcttgt
     9421 cagcatatcg ttcggtcatg atagatttag ctccttaact tcaataaaat tgggtaaact
     9481 gttttattaa tgagtcacat tacccaaaag tgtttaattg acatccctat tttaacagaa
     9541 aaacagaaaa atttcagttt ttctgtcgct tttagccatt atttatcgtt gaactttttt
     9601 aaggtattaa tacaccgagc tactttactt ttagcatatt tgaaagtaat gtgatgttca
     9661 gataaaaaag aatcaaaatc aatctttcct tgaagagcgt cacttacttc taattctaat
     9721 tcatagtctt tggtattggc atataggtta tagtctaagg ccaatttacc tatcggtatc
     9781 actgtctcgc gacgaacggt agttaaattg ccaaaagtta ccagtgcaga tggtttgata
     9841 ccttttgaaa taataatatc aagagcagtt gattcaggaa gattcccatg tttaatcata
     9901 tcttttgctt gttcaagaaa aagagggaca ttgtactcac gattccccac tttttctggc
     9961 acttttaggg ttaattcggc gctatttaca aatgttcgaa tacgtaatga cattttgtta
    10021 gcttttaaat caaatgcttt tgtatcaata tagtaattag tttgtgtaac aggcgtcaca
    10081 tgtttcattt gagataaaag acggttatac tcattttttg taagtagagt tttatattcg
    10141 atttctaggt tagtcatggg aaattccttt taatttgtgc tataataaat tgttatttca
    10201 attttatatt attatcctgg ctttgacaag aaaggagaca atagatgacc ttagattggg
    10261 aagaattttt agatccttat atccaaacag ttggtgaatt aaaaataaaa ctacggggta
    10321 ttcgcaagca atatcgtaag caaaatcgtt attctccgat tgaatttgtt acgggacgtg
    10381 ttaagtcaat tgaaagtatt aaagaaaaaa tgattttacg tggtgttatt gaagaaaata
    10441 tagcacaaga tattcaagat attgctggac tacgtattat ggttcagttt gttgatgatg
    10501 tggaagaagt cttagctcta ttgcgtcaac gacaagacat gacaattgtt tatgaacgag
    10561 attatattcg caatatgaag tccagtggct acaggtctta tcatgtggtg gttgagtacc
    10621 ctgttgatac tattgaaggt caaaaaaagg tcttagcgga gattcagatt cgtactttgg
    10681 caatgaactt ttgggcgacc attgagcatt ctttaaatta taaatatggt ggagacttcc
    10741 cagaagaaat taaaaagcgt cttgaagtga ctgctaaaat tgctttagaa ttagacgaag
    10801 aaatgcgtaa gattcgagaa gatattcggg aggctcaatt attgtttgac cccgtgacac
    10861 gaaatttaag tgatggtgta ggaaatagtg atgacacaga tgaattatac aggtaaggta
    10921 aagcgagttg ctattattgc aaatggtaag taccaaagta aacgcgtcgc ctccaaactt
    10981 ttctccgtat ttaaagatga tcctgatttc tatctttcaa agaaaaatcc ggatattgtg
    11041 atttctattg gcggagatgg gatgctcttg tctgcctttc acatgtatga aaaagaatta
    11101 gataaggtac gttttgtagg aatccacacc ggtcatcttg gcttttatac cgattatagg
    11161 gattttgaag ttgataaatt gattgataat ttaagaaaag acaagggaga acaaatctct
    11221 tatcctattt taaaagttgc tattacttta gatgatggtc gtgtggttaa agcgcgtgct
    11281 ttgaatgaag cgacggttaa gcgtattgaa aaaacgatgg tagcagatgt tattattaac
    11341 catgtcaaat ttgaaagctt ccgaggtgat gggatttcag tatcaacccc gacagggagc
    11401 acagcctaca ataaatcttt aggtggtgct gtcttgcatc cgacgattga agcgctgcaa
    11461 ttgacggaaa tttccagtct taataaccgt gtctttagaa ccttgggctc atcaatcatt
    11521 attcccaaaa aagataagat tgagttagtg ccaaaacgat taggaattta taccatttcc
    11581 attgataata aaacctatca gttaaaaaat gtgacgaagg tggagtattt tatcgacgat
    11641 gagaaaattc attttgtttc ctctccgagt catacgagct tttgggaaag ggtcaaggat
    11701 gcctttattg gagaaattga ctcatgagat ttgaatttgt agcagacaaa cgaataaaag
    11761 taaaaaccct tttaaaaagt catgatgtct ctaaaggact attggctaaa atcaaatata
    11821 agggtggtaa tatattagtg aatggtattg aacaaaatgc catttattta ttgcaagtgg
    11881 gagatgttgt caccattgac attccaaacg aagagccctt tgaaaagctt gaagccatcc
    11941 cttttgattt ggatattgtt cacgaggatg atcatttttt ggtgattaat aaacccattg
    12001 gctttgccag tattccaagt gccatccatt ccaataccat tgctaacttt atcaaggcat
    12061 attatgtgga caatcattac ctagaccaac aagtacatat cgtgacccgt ttagacagag
    12121 atacgagtgg cttgatgtta tttgctaagc atggttatgc tcatgcaagg ttggacaagc
    12181 aactccaaac tcgctcaata gagaaacgtt attttgctct ggtttctgga aatgggacgt
    12241 tgccagatga aggagatatt attgctccaa ttgggcgttc aaaagatagt attattacca
    12301 gggcagttga ccctatgggc aaatatgcca agactagcta taaggttgtg gcgcgctatt
    12361 cagaaaatgt gcatttagtg gatatcaaat tgcatacagg acgtacccat caaatcaggg
    12421 ttcattttgc ccatattggt tttccacttt taggggatga cctttacgga ggtcgtttgg
    12481 acttaggaat aacaaggcaa gccttacatt gtcattattt gaatttcaaa gatccattta
    12541 cagaaagtga ttgtagctat gcaatacact tgacagatga ctttgatagc gttatcatag
    12601 gtttacagaa aaaagataga ggttattaaa gaatgagtat tcgtagttta ttcggtggtt
    12661 taagagaaaa aattcttggt aaaaacatga agattgtttt cccagaaggt aatgatgagc
    12721 gcgtcgtccg tgcagctgct cgtttgaaat ttgaaggact cttagaaccg attatccttg
    12781 gacagtcaga agaggtacgc aaccttttga caaaattagg cttcgctgat caagactata
    12841 ctatcattaa tccaaatgaa tatgcggatt ttgacaaaat gaaagaaact tttgttgaca
    12901 tccgtaaagg aaaagcaacg cttgaagatg ctgataaaat gcttcgtgat gtgaattact
    12961 ttggtgttat gttggttaaa atgggattag cagatggaat ggtatctggt gctattcatt
    13021 caacagctga tactgttcgt ccagcccttc aaatcattaa aaccaaacca ggtatttcaa
    13081 gaacttcagg tgttttcttg atgaatcgtg aaaatacgag tgagcgttac gtgtttgctg
    13141 attgtgctat caatattgac ccaacagcac aagaattggc agaaatcgct gttaacacag
    13201 ctgaaacagc taaaatcttt gatattgatc ctaaaattgc tatgttaagc ttctcaacaa
    13261 aaggttctgg taaagcgcct caagttgata aggtaagaga agcaacagaa attgctaaag
    13321 ggttgaatcc tgacttagct cttgatggtg aattgcagtt tgatgcagcc tttgttcctg
    13381 aaacggcagc tatcaaagca ccagattcag cggttgctgg ccaagccaat acttttattt
    13441 tccctgactt acaatctgga aatattggtt acaaaattgc acaacgttta ggaatgtttg
    13501 atgctattgg accaatctta caaggattga acaaacctgt taatgattta tctcgtggct
    13561 caagcgctga agatatttac aaattagcta ttattacagc agcacaagct atcgaaagcc
    13621 aagggtaact taagacccct tgatatagga ttctaaccgc taagtgatgt gttagaagtt
    13681 gtcctaagac taattgacat tattaaaaac gagttggcca gaccatctcg ttttttttga
    13741 aggtgaggaa atgtatggga aatattgcct tagtaacagg ggcttcagcc ggttttggca
    13801 gagccattgt cgaacactta attgcagatg ggataaaggt tattggtgct tctcgtcgtc
    13861 ttgagaaact caaggtctta gaaacgagct ttgggtcgga caacttttac cctttgcaga
    13921 tggatgtcac agataccaaa gcgattgatg atgctttgac aagtctacca aatcagtggc
    13981 aagatattac tattttagtg aataatgcag gcttagcatt gggatttgat aaggcttacg
    14041 aagccgattt tgagaattgg atgacgatga ttaataccaa tattgttggg ttgatttatc
    14101 tcacacgtca attattacca catatggtct ccaaagatga tggtattatt attaatttag
    14161 ggtcaaccgc agggacaatt ccctatccgg gagctaatat ttatggggct agcaaggctt
    14221 ttgttaagca gttctcactc aatttacgag ctgatttagc aggtagccat atacgtgtga
    14281 ccaatattga acccggtcta tgtgaaggga cagaattttc aaaggttcgc tttaatggtg
    14341 atgaggaacg ggtgaaggcc atttatcaag gagcacatgc cattcaacct caagatattg
    14401 cgtcgactgt atcatgggtg attcatcagc cccctcatgt taatgttaat cgtattgagc
    14461 tgatgccagt gtcgcaaagt tatggaccgc aaccagttag tcgtgactag caagggatga
    14521 gtccataaga ataataatct ttagaaaaat ggtttactgg tatggattat tctttataag
    14581 tgagacttac cgatttagtc atcaaaaaaa gcaatcaaga agaaggtttt gaggtcttca
    14641 agcgttcatg attgcttctt gtgcgttgat gatttaggct gaactcccta tttaaaatca
    14701 agtttagtga tctaaaatgg aagggcctga tgtgacatcg gaccagccaa caatggcttg
    14761 tccagtttct tctaaatact ggtttaataa cggtttaatg tcttgaacgg tatccaaaac
    14821 tcccaccagc tgaccttgag gatttttaag agcttgataa tgctgcatgt aaatatgatc
    14881 caatgaaccc gaaggaagta ggaaggtttt acaagggtta tctgataaac gcaattcagc
    14941 aagaatccaa tcttctattt ctttatcagt agttggcaaa aaacttccat taaaagcatg
    15001 attatcgact atgatgtttc catttgcatc agataacctt gcttgcaaag gaaggtactg
    15061 taaaaaatgt ccatcaaatt tttggtaaaa tccagtgagg tattggctta acgataagac
    15121 tgctgttgct tgatcaaaga cactttctgt cactaaaaag ccctgaccat gttgagaagt
    15181 tttgaaatag agtgtaggaa agtcaatgtc atgctcatgg aaactgaaaa ttccttggaa
    15241 agtcgacaat tgatttgact gaatgttggc tttgctagtg agaatagtca cctcaaaaag
    15301 gtgtgagaag tggtgggaaa gctctctaat aaacgtctct tcaagttggt tttcaggtgt
    15361 taagagcgct aaaatggaaa tggcattttt cataattgta tgatagctgt attttctaga
    15421 aaagacaatt aatctgctta tctatcatca taggcaagtc taggattttt gataaggtaa
    15481 ttttggagag ataggctgac aaagtgacct gtttttctat aagggggtaa tagtctcatt
    15541 atttgactta tagtgggaaa ctttttataa tggtcaacaa caaacgacaa gaaaataatg
    15601 agagaaaatg tttttgagaa tgggtagttt ttaccttact gagggtccct aagagtccct
    15661 ttaattagta gatgcgtttg tctaaaaatg gaagtgcctg cagtgattcc ttgtagttac
    15721 tcagaacgtt ttgagaaaaa atgacagaga gacaggttga atgaactcat cagcctttat
    15781 gttgatgctt taaccaatga attggagaga taatgatacg ttttaataat gtttctaaaa
    15841 cttttggtca gacaaaggtt ttgcaagagc agacctttca aattaatgac cgtgaatttt
    15901 ttgtgctggt tggccccagc ggatcgggca agaccacact tttaaaaatg attaattgcc
    15961 ttattgaacc aagctctgga gacattttac taaataacgc cccacagaaa gagttagatt
    16021 tacgtgagat gaggctatct attggatacg tattgcagca aattgctctt ttccccaatc
    16081 tcacagtagc tgaaaatatt gcgattatcc ctgagatgaa gcagtgggca cctgaagaag
    16141 ttagacaaaa aactgaagaa ttattggata aggtgggcct acctgctaaa gattatcttg
    16201 atcgttaccc aagtgattta tcaggaggtg aacagcaacg tattggtatt gtacgtgcta
    16261 tcatttctca tcctaaaatt ctattgatgg atgaaccatt ttcagcctta gatcctattt
    16321 cacgtaaaca attacaagag ttaatgttga gtcttcacaa ggaatttgac atgacgattg
    16381 tatttgtgac acatgatatt gatgaggcta ttaaattagg agaccgcgtg gctattttaa
    16441 atgagggaga aattgtgcaa ctggatcgcc cagaaatgat taagacccac cctgctaatg
    16501 cttttgtggc caatttattt ggaggtgatg agcatgccta gtttgtttat cacctttcaa
    16561 aaccgtttta atgaatggct ggcggctctg ggggaacacc tgcaaatttc ccttttatct
    16621 cttatgatcg ccttgctaat aggtgtgcct ttagcagccc ttctcagtcg cagcaaacgt
    16681 tggtcagaca ttatgttaca ggtaacaggt gtttttcaaa ccattccctc actggctttg
    16741 cttggtcttt tcatcccttt aatgggaatt ggaacgttgc ctgcagtgac agctttagtt
    16801 atctatgcga tttttccgat tttacagaac accatcacag ggttaaatgg tattgaccca
    16861 agtctcgtgg aagcagggac agcgtttggg atgaccaaat gggagcgatt gaaaacattt
    16921 gagattccaa ttgccatgcc tgtgattatg tcaggtgtgc ggacgtcagc agtcatgatt
    16981 attggcacag ctactttagc ttccttgata ggagctggtg gacttggctc tttcatctta
    17041 ttggggattg atcgtaataa tgctaacctg attctgatag gggctatttc ttcagctctg
    17101 ctagctatta ttttcaatag tttgttacag taccttgaga aagcttcctt gcgacgaatt
    17161 atgattagtt ttggaatcac ctttcttgtc ttgttggcat catatactcc tatggtgctt
    17221 agtcagtttt caaaaggaaa agatacagtg gttattgccg gtaaattggg agcagagccc
    17281 gatattctga ttaatctcta taaggaatta attgaagacc aatcagatat aagcgttgag
    17341 ttgaaatcta attttgggaa aactagcttc ttatatgaag ctcttaaatc tggagatatt
    17401 gatatctatc ctgaatttac aggaaccata acatcaagtc ttttacgtga caaaccacct
    17461 ttgtctaatg accctaagca ggtctatgag gatgctaaaa aaggcattgc taagcaagat
    17521 aaactgaccc ttcttaagcc gtttgcttac caaaatacgt atgctgttgc tatgccagaa
    17581 aaattggcag aggaatatca gattgaaacc atttctgatt taaaagcgca tgctgatact
    17641 ttaaaggctg gttttactct ggaatttaag gacagagcag atggctataa gggaatgcaa
    17701 tctcaatatg ggttacagtt atctgtggcg acgatggagc cagctcttcg ttatcaagca
    17761 attcaatcag gagatatcca agtaacagat gcttactcta cggatgctga aattacgaaa
    17821 taccatttga aagttttaaa agatgataaa cagttgtttc caccttatca gggagctcct
    17881 ttgatgaaag cctccttatt aacaaaacat ccagaattaa aaggcattct taatcaatta
    17941 gcgggaaaaa ttactgaaaa agagatgcag gacatgaact acaaggtatc tgttaaagga
    18001 gcagatgcta ataaggtcgc tcgagattat ctgttgaaag cgggattaat ccagaaataa
    18061 acagaaatgt tggtccgtag gactggcatt ttttcgtctc ttacgttcaa gaagtaacaa
    18121 ttcataaata caaaaaccga acatttaaat taaaaatagt atttaaattt gacaaaaaat
    18181 aaacaatgaa ataaactata aaaggaaagg taagttttta cctagcgatg cctctaatca
    18241 tcagaggcca taggaaggtc attttgtata aaggacgtgg agaagaagat gagaactgac
    18301 ttattttctt tcatctgtgt ctaaagagga gaaacttatg tttaatgaca tccctgtatt
    18361 tgattatgaa gatattcaac tgattcctaa caagtgcatt attactagtc gttcacaagc
    18421 agatacaagt gtgacactcg gaaaatacca gttcaagcta ccagttatcc cagcaaatat
    18481 gcaaaccatt attgatgaaa caattgctgg gcagttggca aaagagggtt atttttatat
    18541 tatgcatcgt tttgatgaag atagccgcaa accttttatc aagcgcatgc atgaacaagg
    18601 cttgattgca tcaatctctg ttggggttaa agcttacgaa tatgaatttg tgacttcttt
    18661 gaaagaggat gctcctgagt ttattaccat tgatattgct catggtcatg ctaacagtgt
    18721 tattgatatg attaagcata tcaaaactga attaccagaa acatttgtca ttgctggaaa
    18781 tgttggtacg ccagaagctg tacgagaact cgaaaacgca ggtgctgatg ctactaaagt
    18841 aggcatcggt ccaggcaaag tatgtatcac taaagtaaaa acaggtttcg gtacaggtgg
    18901 ttggcagtta gcagcactgc gctggtgcgc aaaggcagct cgtaagccta tcattgctga
    18961 tggtgggatt cgcacacatg gtgatatcgc taaatccatc cgctttggag ctagtatggt
    19021 catgattgga tcgctttttg cagggcatat tgaaagtcct ggaaaaacag tagaagtgaa
    19081 tggtgaaacg tttaaagaat attatggctc ggcgtcagca tatcaaaaag gcgagcataa
    19141 aaatgtagaa ggcaaaaaaa tcttattgcc tacaaaaggt cacttatcag ataccctgac
    19201 cgaaatgcaa caggatttgc aatcgtccat ttcttacgcg ggtggtaaag atttagacag
    19261 tttgcgtcat gttgattatg ttatcgtcaa aaattctatt tggaatggtg attccattta
    19321 aatgatatgg agttagctag gaccagtaaa gaatttttta gcttaatgct ggagatgctt
    19381 ttatcatatc tctgtaggta ttgaaaaaaa taagaaacat ggtaaaatag taaattatca
    19441 taatgaattc aataatgaca tacttattta tgctgtgaat tggcgcagcg tctctacaag
    19501 acaccttaat gtctaacaat aagtaagctt ttaggcttgc ttggttttta accttgcaaa
    19561 gtgagaaact cttatagttt ctggctttgc ggcaggcttt ttctatttga atcaaaagga
    19621 gatttatgca gttacttgaa gaacgcatct taacagatgg caatattcta ggagagaata
    19681 ttctaaaggt agataatttt ttaactcatc aagttgatta ccggttgatg aaagcaattg
    19741 gtaaagtgtt tgctcaaaaa tatgctgagg ctggcattac aaaagtggtt acaatcgaag
    19801 cttcaggtat tgcaccagcc gtatacgctg cagaagcaat ggatgttcct atgatttttg
    19861 cgaaaaaaca taaaaacatt accatgacag aaggcatttt gacagcagaa gtttattctt
    19921 tcactaaaca agttacgagc acggtgtcta tcgctggtaa attcctatct aaagaagaca
    19981 aggttttgat tattgatgac tttttagcta atggtcaggc agccaaaggc ttgatcgaga
    20041 ttattggtca agcaggggca caagtcgtcg gcgttggtat tgtgattgag aaatctttcc
    20101 aagatggtcg tcgattgatt gaagatatgg gcattgaagt gacctcatta gcccgtatta
    20161 aaaactttga aaatggtaat ctaaacttct tggaggctga cgcataatga cacattcaac
    20221 aaagcaggaa cattctcact cgcaatcagc tgtccttggc ttacagcatg tgctttcgat
    20281 gtatgccgga tctattttag taccaattat gattgcaggt gctttaggtt attccgctag
    20341 agagttgact tacctgattt cgacagatat tttcatgtgt ggtgtggcta ctttcttaca
    20401 gttaaagtta accaagcata cgggagtggg attaccagtt gttttaggat gtgcttttca
    20461 atcagtggca cctctatcca ttattggagc gcagcaaggt tcaggtgcca tgtttggagc
    20521 cctcattgcg tcaggaattt atgtcatttt agtggcaggt attttttcta agattgctcg
    20581 tttcttccca ccaattgtta caggatcggt cattaccgtt attggcttaa gtctcgtagg
    20641 tgttgctatg gggaatatgg gagataacgt caaagagcct acggcacaaa gcatgatgct
    20701 atcattgttg acgattgtca ttattctttt agtccaaaaa tttaccaaag gttttgtgaa
    20761 atccatttcc attttgattg ggcttgtagc gggaactttg gtttcagcca tgatgggatt
    20821 agtagataca actcctgttg tagaggcttc ttggattcat gtgccaaccc cattttattt
    20881 tggcatgcca acttttgaaa tcacttctat tgtgatgatg tgtatcatcg caacggtttc
    20941 tatggttgaa tcaacaggtg tctacttagc gctttcagat ttgactaatg atcaattaga
    21001 tgaaaaacgt ttgcgcaatg gttatcgctc agaagggatc gcagtctttc tcggtgggtt
    21061 atttaatacc ttcccttaca ctggtttttc tcaaaacgtt ggacttgtcc aaatctcagg
    21121 tattaaaacc cgtcgtccga tttactacgc agcaggtatc cttgtggtca ttggtctact
    21181 ccctaaattt ggagcaatgg cacaaatgat tccaagccca gttcttggtg gagccatgct
    21241 tgttctcttt ggtatggttg ctcttcaagg aatgcaaatg cttaatcgtg ttgatttcca
    21301 aaagaacgag tataatttta ttattgttgc cgtttccatc tcagcaggtt taggctttaa
    21361 tggcactaat ctttttgcca gcttaccaga gacagctcaa atgttcttaa cgaatggtat
    21421 tgtgattgcc actttgacat ctgttgtctt aaatttagtt ttaaatggca aagacaaaca
    21481 agatgaataa ttaaaaaacg gctaattgag tcgttttttc tgtgatcagt tttgagcaaa
    21541 ccgttaaccc cttttaatga tagtatggtt gaaaatagtg tctcaaacca tttgacataa
    21601 ggacatggtg ctttcttgtt actagaagac cttcaatatt atcaaaggta tttaaaagtg
    21661 tgataatcat tgagctatca agaccaaata aacgggtagt ccatatgtca caataaaaag
    21721 aagaaggagc catgatcgtt aagctaagca tatctgtttc tatagggtag cctgtctgac
    21781 gatctaagat atgatgatac tgtttccctt tggctgtaaa ctgcctttca taaattccag
    21841 aagtcacaac agagtgattg ttaaccttga tgacaccaag atgttgccca cgtttagcat
    21901 ctggtttttg aatgccaatt cgaaaaatct tgtcagggct tttcggatta ggtccatgaa
    21961 cgcgtacatt tccacctaaa ttaataaggg cagagtcaat accatcttca atcaggtagg
    22021 ccataatctt atcagtaata taaccttttg ctaaggctcc taagtctaaa gccattccta
    22081 cttgttgtaa aaagacagtc tgttttttgt catctataag aacctgtcta ggatctgtta
    22141 gagctaactg ttgtgagata aggttatgac taggcactct agcatcttca aatccaattc
    22201 gccaggcttg aacaagtggc ccaatggcga tattaagatt acttggtgtt gacaagctat
    22261 gagctttacc aatttggact aggttaaata aatcagagtg gacgctgacg ggtttaacac
    22321 cagccgcctg attaatagcc atcaattcag agtttgaatc attagctgaa aaacgatttt
    22381 tgtaagtata taataagtca atgactctac ttagttgttg gcaagctttg tcagattcga
    22441 tctgaatatc aataactgtt cccattagct ttaattgttg cgtaacaaac ataattagtc
    22501 ctctttttag cagaataccc ccactaagga gtgggggaag gtcattatga tttttgtttc
    22561 atttcacctt gaggatagta agttccttca ggcatatcgt tgataaaaac atggatattt
    22621 tctttaggtg cttttgcaat gcgtgatact acttcggtaa cctcgcgagc taattgattt
    22681 ttttgttctt gtgagcgtcc ctcaaacaag tcaattgtta caaatggcat aatgttactc
    22741 ctttatatta ttgggcatca ttgtaacata atgctatgaa aaagtacaca ctgttttcaa
    22801 tggctgggaa aacttcagcc tttgtggtaa aataggtaga atactataga aagtaggatt
    22861 ttccgctttg gctcaactct attataaata tgggacaatg aattctggta aaaccattga
    22921 aattttaaaa gttgctcata actatgaaga acaaggcaaa cctgttgtca ttatgacaag
    22981 tgctcttgat acacgtgatg gttttggcat cgtctcaagc cgtattggta tgcgacgaga
    23041 agctattccc atttcaaatg acatggatat tttcacattt attgcccaat tggaagaaaa
    23101 accttattgt gtcctgattg acgaaagtca atttttaagc aagcaaaatg tttatgattt
    23161 ggcgcgtgtg gtggatgagc taaatgttcc tgtcatggct tttggtttga aaaatgattt
    23221 ccaaaacaat ctttttgaag gttcaaaaca tttgctatta ttggccgata agattgatga
    23281 aatcaaaaca atttgccaat actgtagtaa gaaagcaacc atggttttaa ggacagaaaa
    23341 cggaaaacct gtctatgaag gtgatcagat acaaattggt ggtaatgaaa catatatccc
    23401 agtttgtcga aaacattatt ttaatccaga gatttaaaag gtaatcggtt ttaaaggaga
    23461 tacaaagaaa aatgaatatt tatgatcaat tgcaggcagt tgaagatcgt tatgaagagt
    23521 taggtgagtt acttagtgat ccagatgttg tttcagatac aaagcgattt atggaattat
    23581 cacgtgaaga agctaacact cgagaaactg tcacagctta tagagaatat aagcaagtca
    23641 ttcaaaccat ttcagatgct gaagaaatga tcaaagatgc cagtggtgat cctgaattag
    23701 aagaaatggc taaagaagaa cttaaagaat caaaagcagc taaagaagag tatgaagaaa
    23761 aattaaaaat ccttctatta cctaaagatc ttaacgatga taaaaacatt attttagaaa
    23821 ttcgtggtgc tgctggtggc gatgaagcag cgctttttgc tggtgatctt ttaacaatgt
    23881 atcaaaaata tgctgaaact caaggatggc gttttgaagt tatggaatca tccgttaatg
    23941 gtgttggtgg tattaaagaa gtggttgcca tggtttctgg tcagtcagtt tattctaagc
    24001 ttaaatatga atcaggagct catcgtgttc aacgtgtccc ggtgacagag agtcagggtc
    24061 gtgttcatac gtcaactgca actgttttgg taatgccaga agttgaagaa gtagaatacg
    24121 acattgatcc taaagacctt cgcgtcgata tttaccacgc gtctggtgct ggtggtcaga
    24181 atgtcaataa agttgctact gcagttcgta tggtacacat tcctacagga atcaaagttg
    24241 aaatgcaaga agaacgtact cagcaaaaaa accgtgataa agcgatgaaa atcattcgtg
    24301 ctcgtgttgc tgaccatttt gcgcaaattg ctcaagatga acaagatgct gaacgtaaat
    24361 caacagttgg tactggtgat cgttcagaac gtattcgtac ttataatttc ccacaaaatc
    24421 gtgtaacaga tcatcgtatt ggtttgacac ttcaaaaatt agatactatt ttatctggaa
    24481 aaatggatga agttattgat gcactcgtta tgtatgatca gacaaagaaa ttagaatctc
    24541 tcaataacta atgaactatg ctacattaat cagaacttat gaagataaac tagaacaaat
    24601 agatgaggat cgcgaaaatc tcgcctatgt ttttagcgaa atcaaagaat ggtcaagtct
    24661 agatatgttg attcaccaaa atcaagctgt gactcctgaa gatgctgttc ttttggaaca
    24721 tattttctgt tctctaagtc agcatttatc tcctcaatat attacaggta atgcttattt
    24781 tcgtgatttg aagcttgcag tcgacaagcg tgtattaatt ccaaggccag aaacagaaga
    24841 attagttgac atgattttag ctgaaaatct tgatgcaccg ttgaatgttt tagatattgg
    24901 tacaggtagt ggagcgattg ctatttctct caaaaaagaa aggcctaact ggcaagtaac
    24961 ggcatctgat atttctagag ctgctcttga cttggcaaaa gccaatgctg atgcttacca
    25021 gcttgatata acgtttattg aatcagatgt ttttagtctc atttcaggaa cctttgatat
    25081 tattgtgtca aatcctccct acatatccta cgaggataag gaagaagtca gtctaaatgt
    25141 tttgcaatca gagcctcatt tggcactttt tgctaaagaa aatggctatg ctatttaccg
    25201 taagattatt gaacaagctg acaattactt aactaaggag gggaagcttt actttgaaat
    25261 tggttataag caagctgaag gtataaaaga tatgttacaa gcctattttc ctcaaagaca
    25321 tatacgagtc gtaacggata tatttggcaa agaaaggatg gtggttgttg attatggagt
    25381 atctagctag tatcattgaa tcaggtgatg cccttgtttt gccaacagaa acggtttatg
    25441 gtctttttgc caaggcttta gatgagaaag cagtaaatgc tgtttatgac ttaaaacaaa
    25501 gaccccgaga taaagcaatg aaccttaatg tggctgattt taatagtatc ttagcctttt
    25561 caaaagaaca gccgagatac ctcaagaaat tataccaagc ctttttacca ggtccgttaa
    25621 cgattatttt aaaagctaat gaccaggttc cttattggat taattcgggt ctttcaacgg
    25681 ttggttttcg cttacccagt catccaatta ccgcagcttt aattcaaaaa acaggccctt
    25741 taattggtcc ttctgcaaac ttatctggca aagctagtgg tcgagttttt gatcatatta
    25801 tgcaagattt tgactttcaa gtgtttggtt atgcagatga tccttttcta acaggtaagg
    25861 attcaaccat cttagactta tcaggtgagc gtgctgtgat tttacgtcaa ggtgctatca
    25921 ccaaagaaga attattagcc aacgtgcctg aacttcgctt ttaagagagg agccgcttgg
    25981 aatgttgaga ccattaagta aaacggactg tcctatgcta caagaaatta acgccaaagc
    26041 ccttggctat ttagtttcgc ttgacttgtt agaaaggcaa tatgaacgat tgattgagga
    26101 ttgccatcac tattttttag cttatgccga caaggatacc aatcaattat taggctatgt
    26161 tcacgctgaa cggtatgaga ccctttatgc ttctgatggt cttaatctct tgggcttggc
    26221 tgttttacca gcctatcaga gaaggggaat tgggagtgcc ttactaaggg ccctggaatc
    26281 ccaagctagg caggaaggca ttgcctttat tcgcctcaat tctgccagtc atcgtaaaga
    26341 agcgcatgct ttttaccgaa acctcgacta tgcagatgat aaaacacaat tacgattcat
    26401 caaaaactta caaggagatg acaatgattt ttgataaagg taatgttgaa gactttgata
    26461 aagagctttg ggatgccatt catgctgaag aagaaagaca agaacatcat atcgaattga
    26521 ttgcatctga aaacatggtt tcaaaagctg tcatggcagc tcaaggttct gttttaacca
    26581 ataagtatgc agaaggctat ccaggtaatc gctattatgg tggaacagaa tgtgtggata
    26641 ttgtcgaaac gcttgctatt gaacgtgcta agaaactttt cggtgccgca tttgccaatg
    26701 ttcaagccca ttccggaagt caagccaatg cagcagctta tatggcctta attgaggctg
    26761 gtgataccgt attaggaatg gatttggctg caggtggtca tctcacgcat ggctctccag
    26821 taaatttttc tggaaaaacc taccattttg tgggttactc agtagatgct gatactgaaa
    26881 tgttaaatta tgaggcgatt ttagaacaag caaaggcggt ccagccaaag cttatcgttg
    26941 caggggcttc ggcctactct agaagcattg attttgaaaa gtttcgtgcc attactgatc
    27001 acgtaggtgc ttacttaatg gtggacatgg ctcacatcgc tggccttgta gcagcaggtg
    27061 tgcacccgag tcctgtccct tatgcgcata ttgtgacgtc aacaacccat aaaaccttac
    27121 gtggtcctcg tggaggcttg atattaacca atgacgaagc tcttgctaaa aagattaatt
    27181 cggccgtttt ccctggttta caaggaggac ctctggaaca cgtgattgct gccaaagcgg
    27241 ttgccttcaa agaagccttg gttccagcct tcaaagacta tgcacaagct attattgata
    27301 atactgcggc tatggcagca gtttttgcac aagatgaccg ttttcgtctg atctcaggtg
    27361 gtacagataa tcatgtcttt ttagtagatg tcactaaggt tattgctaat ggtaaactag
    27421 cgcaaaatct tctggatgag gttaatatta cccttaataa aaatgccatt ccttttgaaa
    27481 ccttgtcgcc atttaaaacc tcaggcattc gtattggttg tgctgccata accagccgtg
    27541 gtatgggtgt taaagagagt cagaccattg cccgtcttat catcaaagcc ttggttaatc
    27601 ataatcagac tgttattctt gaagaagtcc gtcaggaagt gcgtcagtta accgatgctt
    27661 tcccactcta taaaaagtaa ggaatcatgt tagatagtta tataaaacgt attgtcattc
    27721 atcaattttc tcccaatgac actgagttac tgttatcaga tcgtttagtg agcattacac
    27781 cgcgaattga tgaatatttc cggaaaaaat tggccaaagt gttctcggac gaagccaaac
    27841 gtggtcagtt tgaggctaat aatacctttt ttacaaccat tggtgatgat ttgttggaaa
    27901 cgtctgttac cattgcccag ttatggaagg aagcctttgt tatttcagag gatcaaaaaa
    27961 ccaatgattt agtctttgtc cagtttgata aggatggcga accctttttt gctttcttgc
    28021 gaattgcttt gaaagaacag tttgctcacc tttctgacaa ttatgaacat ccatttacgg
    28081 tgacgcagaa taatctgccg agtcccaccc agacaccaga tgaagcacta gtcattaatc
    28141 tcaaatctgg tcagtactac ttgattgaaa agcgtgtgaa gcataatggc agttttgcta
    28201 attatttttc agaacacctc ttgaaagtta cacctgagca atctgttaaa aagtctatca
    28261 aaatgataga acaaaccgct caaaaaattg cagagcattt taatcaagat gattttactt
    28321 ttcagtcaaa gatgaaatcc actcttttta agcagttaga agcagacgac gttttatcac
    28381 cagaaaaatt agctgaccaa ctctttgatg ataatttaac agcccgttta acctttgtgg
    28441 gtcaagtcaa agaagtcatt ccagaaccta taaaaattag cgatattgat cattctagac
    28501 agataaaaaa actagaaaat caaaagctat cgttatcaaa cgggatagaa ttaacagtac
    28561 caaatgccat ttatcaggat gctgaagcgg ttgaattttt gttaaatgat gatgggactt
    28621 attcaatctt gattaaaaat atagaggata ttaaaaccaa atagatgttt agacttttaa
    28681 agcgtgcctg cagttttctt cttctttttg tcatttatca atcctttgtt attcatcaca
    28741 atgttcaacg tgttttggca tataagccta tggttgagaa aacattagca gaaaatgata
    28801 caaaggcaaa tgtagactta gtcttagcta tgatatacac cgagacaaaa ggaggagaag
    28861 cagatgtcat gcaatccagt gaaagcagtt caggacaaaa aaactcgatt acagatagtc
    28921 aggccagtat taagcacggg gtgaatttac tgtctcataa tcttgctcta gctgaggaag
    28981 caggagtaga ttcttggacg gctgttcagg cctataattt tggaacagct tatattgatt
    29041 atattgctaa acatggaggt caaaataccg ttgatttagc gactacctat tccaaaacag
    29101 ttgtggcgcc aagcctaggc aacactagtg gtcaaaccta tttttattac caccccctcg
    29161 ccttgatttc tggaggtaag ctttataaga atggcggcaa tatctattac tcacgagaag
    29221 ttcatttcaa tctttattta attgaactga tgagcctttt ttagaaagtt atcatgaaac
    29281 gattacgtcc atatttgaaa gggtacctaa aagaaagtat cttaggtcct ctttttaaat
    29341 tattagaagc tttatttgaa ttattagtcc ctttgttaat tgctaacatg attgatatat
    29401 cgattagtca acacaacagc cagggaattt tgagggttgt tttaacatta tttggtttag
    29461 caaccattgg cttattgatt tccgttacag cccagtattt ttcttcgaaa gcagctgttg
    29521 gttttacaag acaaatgaca gatgatttgt ttaaaaaaat catgtttttg agcaaggagg
    29581 accaagacca tattggttat gctagtctgt tatcacgatt gaccagtgat agttttcaaa
    29641 ttcaaactgg tatcaatcaa tttttgcgtc tttttctaag ggcccctatt atcgtatgtg
    29701 gtgctatggt aatggcttat tggattagtc caagtctaac cctatggttt gtgatgatgg
    29761 tgattgtctt actaacactt gtttttgtta tgtcacattt attagatccc ctttatctct
    29821 taatccgccg agaaacagat cacttggttc gtttgactag ccaacagtta caaggaatcc
    29881 gagtaattaa ggcttttaat caaacccaaa aagaactcca agcatttaag gagcaaaata
    29941 tgctcttaag tcgccaccaa tatcaagctg ctaccctagc caatgtcttg aatcctatga
    30001 cttttttagt ggtcaatctt actctgttaa tcttgatttg gcaaggaagt tggcaagtag
    30061 ctcatagatc gcttagtcag ggcatgttgg ttgctttaat taattacctc ttgcagattt
    30121 tggcagaact tttgaaaatg accatgttga tgggaaccat caatcaaagt gtaacagccg
    30181 caaaacggat taatcaggtg tttgttttag ctgatgaagc gcctttgccc ttacttaaag
    30241 atggtcctat ttcaactcat ctgttgacca tccggcactt gacctttact tatccaggag
    30301 cagcagagcc aagtctgtat gatattcagt taagtgctga tcagggagaa tggattggca
    30361 tcattggtgg tactggagca ggcaaaacga ctttgattga cttgatctgc caaacttatt
    30421 cacaatatag tggtgagatt agtcttaatt ggcaaggaga agtgccaaaa acactaaccg
    30481 agtggcgaaa tgtgattgct cttgttcctc aaaaagctca attatttaaa ggaacgatta
    30541 gaagtaacct cctactggga caatcaatgc ctattaatga tgaggagctg tggcgggctc
    30601 ttgaattggc tcaagcaaaa gagtttgtcg ctgccttacc agaacaatta gaggcacctg
    30661 ttgaagcctt cggccgtcat ttttcaggag ggcaacggca acgtctagcc attgccagag
    30721 cacttctgaa gccaaaaccc attttgattt tggatgatgc cagttccgcc ttagacaatg
    30781 agactcgagg acgacttttt aaagttctaa aagaagagtt atcagatgtt ttagtgattc
    30841 tagtcacgca atccattaag aatttgcagt ttgctgataa gattttagtg ttggaacagg
    30901 gccatcaatt ggattttgcc agtcatgacc agttaaaagt aagcaatgca ctttatcaag
    30961 aaatgctagc gctttacgtg aagggggagc aaaaatgatt aaaacagatc atcacctcct
    31021 aaagcgagtg cttcaggatt tgttaaaaaa acctctacct gtttgtattc tcgtgatcgc
    31081 tagttttgta caagttggtt taagtgttta cttgcctgtt cttatcggta aagccgttga
    31141 tatgagtttg tcagtcaatt cttggcaaac tttaaaatgg ctgttggggc aaatgttagt
    31201 tattattgtg gtcaacacat tgattcaatg ggtgatgcct cttgtctata gtcgtttgct
    31261 atatcaatat agccagcaat tgaaagataa gcttttggaa aaaattcatc gtcttccttt
    31321 tgcatatcta gatcgtcaaa ccattggaga tttggttagt cgagtgataa ccgacactga
    31381 gcaactgatc aatggtctac aaatggtttt taaccagttt atacttggac tattgacgat
    31441 tctgtgtacc attatagcta tggcacagat tgactggctc atgttaatct tggttttggt
    31501 gttaacacca agctccctct ttttagctcg ttttattgct caaaagagtt ttcattatgc
    31561 acaagcacag accaaaagca gaggaaattt agctcagttt actgaagaaa tccttcgtca
    31621 agaaggtttg gttcaactgt ttaatgctca agagcagtct atctgtgatt atcatgtttt
    31681 aaataaaacc tattgcgagg cttcacaaaa agccattttt tacgcctcaa cagtcaatcc
    31741 agccactcgc tttattaata gtgtcattta tgctttactg gcaggtttag gggctgttcg
    31801 tatcatggct ggactttttt cagtaggtca gctaaccact tttttaaatg ttgttgtcca
    31861 atataccaaa cctttcaacg atatttcatc tgtcttggca gagatacaga gttctttagc
    31921 ttgtgcgcaa aggctttatg accttttaga tattgaaata aaagagcaag agcactttct
    31981 aacatttaag gcatcagcgg ttaaggggca aattgacttt gaagaagtaa gtttttctta
    32041 tcaaaaagac aggcccttac tgaaagatat taatttttct gttcctgcgg gttccaaggt
    32101 agcgattgta gggcctacag gtgcaggcaa atcgacttta atcaatttac tcatgcgctt
    32161 ttatgaactt gatgttggta gcatcaagtt agataaagtt cctattaagt gttatgctaa
    32221 ggaagaactt aggtccatta ctggcatggt attgcaagaa acctggttga aagatgcgac
    32281 tgttcatgag ttgattgctt acggcagtga agaggctagc cgtgatgaag tagtggcagc
    32341 agccaaagca gctcatgcac acttctttat tatgcaactt cctaagactt atgatactta
    32401 cttaagtgct tctgatgatg ctttgtccca agggcagctc cagttattag ctattgccag
    32461 aatgtttttg aaaaaaccaa aagtcttgat cctagatgaa gccacctcct ctattgatat
    32521 tagaacagaa gctgttattc aagaggcact aaaagaactc atgagaggaa ggaccagctt
    32581 tatcattgcc catcgtttat caacgattca atcagctgat ttgattcttg ttatggatca
    32641 aggtcgattg gttgagtggg gaacacatgc cagcttaatg tcaaaaaacg gatgttatgc
    32701 tagattacaa aagatagaat aaagttcgtc ttttaacaaa ggtgaacttt ttaactcttc
    32761 ctaaagttag ttaattaaca aactttaaac tgttatataa aaaaataaca gcgaaaaagc
    32821 ggacgatctt tttttataag tgagaaaaaa cagttgacta aagaagaaat aactcaaaaa
    32881 agtgagcaat atatttggaa gcgttttttg actgtggtat aatagagccc tatataatat
    32941 tgtgaaatga ggaacaaaat atatgagtaa aatcgttgtt gttggtgcaa accatgctgg
    33001 tacggcctgt atcaagacta tgttaacaaa ctacggtgat gctaatgaga ttgttgtatt
    33061 tgaccaaaac tcaaatattt catttttagg ctgtggtatg gcactttgga ttggtgagca
    33121 aattgctgga ccagaaggac ttttctattc agataaagaa gaattagagt ctttaggggc
    33181 taaagtttac atggaatcac ctgttcaatc aatcgactac gatgccaaaa cagtgacagc
    33241 gcttgttgat ggtaaaaacc acgtggaaac ttacgacaag ttgatttttg caactggctc
    33301 acaacctatc ttaccaccga ttaaaggtgc cgaaatcaaa gaaggatcac ttgaatttga
    33361 agcaactctt gaaaatcttc aatttgtgaa gttataccaa aactcagctg atgtgattgc
    33421 aaagcttgaa aacaaagaca ttaaacgtgt agccgtagtt ggtgctggtt acattggtgt
    33481 tgagttagct gaagctttcc aacgtaaagg taaagaagtg gttctgattg atgtcgtgga
    33541 cacttgcttg gcaggttatt acgaccgtga tttgactgac ttaatggcta aaaacatgga
    33601 agaacatggt attcaactag cctttggtga aacagttaaa gaagtagctg gtaatggtaa
    33661 ggttgaaaag atcattactg acaaaaatga gtacgatgtg gatatggtta tcctcgccgt
    33721 tggtttccgt ccaaacacaa ctcttggaaa tggtaagatt gagcttttcc gtaatggtgc
    33781 tttccttgtc aataaacgcc aagaaacttc tattccaggt gtttatgcta tcggtgactg
    33841 tgcaactatc tacgataatg ctactcgcga tacaaactac attgctttag cttcaaatgc
    33901 cgtccgtaca ggaattgtag cagcacataa cgcttgtggt acagaccttg aaggaattgg
    33961 tgtccaaggc tcaaacggta tttccattta cggcttgcac atggtatcaa ctggtttgac
    34021 tcttgaaaaa gctaaacgac ttggttttga tgctgcagtg actgagtata ctgataacca
    34081 aaaacctgaa tttatcgaac acggtaactt cccagtaacc attaagattg tttacgataa
    34141 agactcacgt cgtatcttgg gtgctcaaat ggcagcgcgt gaagatatgt caatgggaat
    34201 tcacatgttc tcacttgcta tccaagaagg cgtaaccatt gaaaaattag ctttgacaga
    34261 tattttcttc ttaccacatt tcaacaaacc ttacaactac atcacaatgg cagcacttgg
    34321 tgccaaagac tagtatctga taggaaaaag ccagagcagt tcagcagcac tggctttttt
    34381 gctttttaat tgttgcaacc tgaaaagttc taaacttatg tttatcctct tatagcacgt
    34441 tggaaaagga atatcaggca aaacaaaaag agactgaagt ctcttttgtg attagttttt
    34501 ggcagcagaa gcaaattctt ccttagcgaa agcttcatcg atgatagctt ttaattgatt
    34561 tgcagaggct tgcatttttt gcaattcagc atcgttaagt ggaatgttta ctggacgaac
    34621 aattccatac gctccaacga tagctggttg accaatatag caatcttcaa caccttcgta
    34681 ttggccttct tggaaaacag aaagcggaag aacagcattt tcatcatcaa gaatagcttt
    34741 tgtaatacga gctagggcaa ctgcaatacc gtagaaagta gcaccttttt tatcgatgat
    34801 tgagtaagca gcatcacgta cagaaatgaa aagatcaact aggccttgct catcaatgtc
    34861 acgattagct tggagccagt cgtaaagacc tacaccagca acgttagcgt gtgaccaaac
    34921 agcaaattca gagtcaccgt gttctcccat gatgtaagcg tgaaccgaac gcgcatcaac
    34981 accgatttta gcagccagag cttgacggaa acgggctgaa tctagagatg tacctgaacc
    35041 aatgacacgc tctttaggga aacctgagaa tttccatgtt gagtaagtca aaacgtcaac
    35101 tgggttagca gcaacaagaa agattccttt gaaaccagat gcaacgattt gtgtaacaac
    35161 ctctttattg atacgaaggt ttttttcaac aaggtcaagg cgtgtttcac ctggtttttg
    35221 aggagcacca gctgttaaga caacaagatc agcatcatga cagtctgagt agtcagctgc
    35281 atagattttt ttaggtgatg taaaagctag agcgtggctt aaatcttctg catctccttg
    35341 agttttttct ttaaaaatat caataatacc aagttcttga gcaatatttt gtgttaccaa
    35401 tgcaaaagcg tatgatgaac cgacggcacc atcaccgacc aagattactt ttttgtgttg
    35461 tttagttgca gtcatttcta aacatctcct taattttatt agagcaaaag ctcatacggt
    35521 cttattttaa catattgata attgtttgtc attgaattca gaaaaataat gaaaatagtt
    35581 tcctgataac gcttccaata agaaaaaata aggaatttat ggtataatga aatctagtat
    35641 tgactagaaa aggagcattc cttaaatgca agatcgaaat ttaattgacg tcaatctgac
    35701 aagtgaaatg aaaacgagtt tcattgacta tgccatgagt gtcattgtgg caagagcttt
    35761 gccagatgtg cgtgatggtc tgaaacctgt gcatcgtcgt attctctatg ggatgaatga
    35821 actcggtgtg acacctgata aaccgcataa aaaatctgct cgtattacgg gtgatgtcat
    35881 gggtaaatat cacccgcatg gggattcttc tatttatgaa gctatggttc gtatggctca
    35941 gtggtggagt tatcgccata tgcttgttga tgggcatggc aattttggtt ctatggatgg
    36001 tgatggtgct gccgctcaac gttatactga agcgcgcatg agtaaaattg cgcttgaatt
    36061 attaagggat atcaataaaa ataccgttaa ttttcaagat aactacgatg gaagcgaaag
    36121 agaaccagta gtattaccag ctcgctttcc taatttatta gtcaatggag cgacagggat
    36181 tgccgttggg atggcaacta acattccgcc tcataatttg gcagagtcta ttgatgctgt
    36241 caagatggta atggaacatc cagactgtac aacaagagag ttgatggaag tgatccctgg
    36301 acctgacttt ccgactggtg cccttgttat ggggcgctca ggtattcata gggcttatga
    36361 tactggtaaa ggttctatcg ttttacgctc tcgaacagaa attgaaacaa ctcaaacagg
    36421 tcgggaacgt attgtggtta ctgaatttcc ctatggcgtc aataaaacga aagtgcatga
    36481 acacattgtt cgtttagcac aagaaaaaag actagaaggt attacagcag ttcgtgacga
    36541 atctagccgt gaaggagtgc gttttgttat cgagattcgt cgagaggcat ccgcgacagt
    36601 tatcttaaat aacctcttta aattaacaag ccttcagacg aatttcagtt ttaatatgtt
    36661 ggctattgaa aatggtgtgc ctaaaatatt gtcactcaga caaatcattg ataattacat
    36721 tagtcatcaa aaagaagtta ttatacgtcg cacccgattt gataaagata aagctgaagc
    36781 cagagctcat attttagaag gcttactgat tgcccttgat catttagacg aagtgattgc
    36841 tattattcgt aacagcgaga cagatgtcat tgctcagaca gaattgatgt cacgtttcga
    36901 cttgtctgaa cgccaaagcc aagctatttt ggatatgcgt ttgcgtcgtt tgactggttt
    36961 ggaacgtgat aagattcaat cagaatatga tgatttgttg gccttgattg ctgacttatc
    37021 tgatatttta gccaaaccag aacgtattat cactatcatc aaagaagaaa tggatgagat
    37081 caagcgtaaa tacgctaacc cacgccgtac agaattaatg gtcggtgaag tcctttctct
    37141 agaagatgaa gacctaattg aagaagaaga tgttttgatt accttgtcaa ataaaggtta
    37201 tatcaagcgt cttgctcaag atgaatttcg tgcccagaag cgtggtggac gtggcgtcca
    37261 aggaactggg gtcaacaatg acgactttgt ccgtgaattg atatcaacaa gtacccatga
    37321 tactttgctc ttctttacga attttgggcg tgtctatcgg ttgaaagctt acgaaattcc
    37381 agaatatggt cgtacagcaa aaggtcttcc aattgttaat ctattgaaat tagaagatgg
    37441 tgaaaccatt caaactatta tcaacgcacg taaggaagaa acagcaggta aatccttctt
    37501 ctttacaacg aaacaaggca ttgtcaaacg tacagaagtt tcagaattta ataatatccg
    37561 tcaaaacggt ctccgtgctc ttaagttgaa agagggagat cagctgatta acgtcctttt
    37621 gacaagcggt caggatgaca ttatcattgg aactcattcc ggatattctg ttcgttttaa
    37681 tgaagcaagc atccgcaaca tgggccgatc agctacgggt gttcgcgggg ttaagcttcg
    37741 tgaagatgat cgcgttgttg gcgcgtcacg tattcaagat aaccaagaag ttcttgtaat
    37801 taccgaaaat ggctttggta agagaacaag tgctacagac tatcctacaa aaggtagggg
    37861 gggaaaagga ataaaaacag ctaatattac ccctaaaaat ggtcaattag ctggattggt
    37921 taccgtagat ggtacagaag atatcatggt gattacaaat aaaggtgtca ttattcgcac
    37981 aaatgttgca aatatttcgc aaacaggtcg tgctactttg ggtgttaaaa ttatgaaact
    38041 agatgctgat gctaagattg taacatttac cttggttcag cctgaagaca gtagtatcga
    38101 tgaaatcaat actgatcgtg aaaatagtat ttcaaaaaat aaagataatt aatcataaca
    38161 aggagtaaaa aatggtaaaa aaacaaaagc gtcgaaaaat aaaatccatg tcttgggcaa
    38221 gaaaactact aatagctgta cttttaattc taggtttagc attgcttttt aacaaaccta
    38281 tccgaaatac attaattgct cgaaactcta ataaatatca agtaacgaaa gtttcgaaaa
    38341 aacaaatcaa aaagaataaa gaggctaagt caacatttga tttccaagcc gtagagccag
    38401 ttagtacaga gagtgtcttg caagcacaaa tggcggctca gcaacttcct gttatagggg
    38461 gcattgccat accagagctt ggcattaatt taccaatttt taaaggttta ggaaatactg
    38521 agcttattta tggcgcagga acgatgaaag aagaacaagt tatgggagga gaaaataatt
    38581 attctcttgc cagtcatcat atttttggaa ttacaggttc atctcaaatg ctcttttcgc
    38641 cgcttgaaag agcacaaaat gggatgtcca tctatttaac agataaagaa aaaatttacg
    38701 aatacatcat aaaagatgtt ttcacggtag ctcctgaacg cgttgatgtt atcgatgata
    38761 cagctggtct caaagaagtg actttagtga cttgtacaga tatcgaagca acagaacgta
    38821 ttattgtcaa aggagaacta aaaacagaat acgattttga taaagcgccc gccgatgtat
    38881 tgaaagcttt taatcattct tataaccaag tatctaccta gatatatctt aaatataata
    38941 tttaaaaagt aggaccatag agaaaattta tctcatccta ctttttttga aaggagaaat
    39001 atgatgaaac taaatgccat tcaccacgtt gctattattg tatctgatta tcatctgtca
    39061 aaagattttt atgtgaataa actcggattt gaaattatca gagaaaatta tcgtcctgac
    39121 aagcatgatt acaaattaga tttatcttgt ggtcgtattg aattggagat ttttggtaag
    39181 gtgacaagtg acccctacta tcaagctcct cctaaacgtg taagtcaacc agaatttaaa
    39241 agtgaggcct gtggtttgag gcaccttgct tttcgtgtta ccaacattga gagttatgta
    39301 gatgatctaa agagcttagg aattccagtg gagcctatta ggcatgatga ttacacaggt
    39361 gagaagatga ctttcttttt tgatccggat ggtttgcctt tagaattaca tgaatagact
    39421 tattaggtta acgattggat cgtgctatta aactcgttaa aaatagttat ttgttgtatc
    39481 ttcttttgct tgattaatct cttttgatcg ttaaatgatt tggttaactt tcaaagtgaa
    39541 gagcaatttt tggagtaatc ttgtcgaagt tttttaatag aataactata ggaaaagcaa
    39601 aatatttgac gataaactct tacagtgtta taatgacaat ataagtaaat aactgtgttg
    39661 tcactttgtt gaaatgtttt cgtattaatg tttttttatc tattacgata gataatttta
    39721 cttcaatttt tgattgaaaa tactaaaata agtgatgtgg tcactttgac aaccatttat
    39781 tggtcctttt agataagaca attttgtggc tcttcagttg aaaggatgtg ttgttgatgt
    39841 tagaggaaac aatcatgtta aattaactca ggcttaaaga ccaaaaaagg agtgaagtct
    39901 attatgaaaa aaagaaattt taaacttaca gaaatgttac aattggtttt attagtggtt
    39961 cctgctgtaa aacttgctaa aaaaatgttt aacgatagta aaacaccgtc taaaagaaag
    40021 taggtctaca atgactaaaa aaaatcatgg cgttttttgg gggttgttgc ttggatcagc
    40081 agcagctagc attgcttact taagtttatc gtcttccaaa aaagatcaac tcttgaaaaa
    40141 tagtgctaaa aaaatagatg atctcaacgc ttacttgcaa gataaaagta agcaagttct
    40201 ggatgcagta tcggaaaaag tacaagaatc caaagatgct gttgaagttt atggtggtat
    40261 tgccgcagaa accgttgaag aaaagtggca agataccaaa gatgctgttg aagtttacgg
    40321 tggtattgcc gcagaaacag ttgaagaatc tttgggacag gctaaagaaa aggtagaagg
    40381 aattggcgaa gctactagtc aaactatcca atctaaaatg gaaaaactca ccagcgataa
    40441 gacagatgct gatgatgatg aaaaataggt tttaaaatac ctttaagata agacaaaaag
    40501 tgccattagg cactttttta atcttctgtt ggaacaataa tcgaaatggc ttttggaatg
    40561 atatctaatt gaatgggcag atagtcactt ttatccccat caatcctggt acgtggtaat
    40621 atcgcttgtt ttttagcttg tggtagtaag ctaagcgtat tggcttgaaa atgactgatt
    40681 tcctctgctt tggaaaaatc accctgttta aaatcattaa tatgccataa aaatttaaag
    40741 aaagagactt ttttcatggt ataaacttgg aaatagccat cgtcagcttg agcaccagga
    40801 gaaaaagaag gaaaaccagc aatggtattt gtcatcgtga ttaagagaaa cttcgttttt
    40861 aggtgaatac gatggttgtg gctaattaaa gagatggcat agctgcgatt tcgtttcagt
    40921 attcgaatac tatcttttaa aaatgctaga ggaccaaagc gtcgtttttc ctcagccgtt
    40981 acgtcggcag caatatcagc tagtaatcct aaggttaaac tggaaatcat gtattgctgg
    41041 ttagctttgc aaatatcaac ttgcttgatt tgtccagtca agatagtatc caacgcctca
    41101 gtaatttgta aagggatatg catagccttg gcaaaattat tgactgtccc tgagggcact
    41161 aagcctatta aacaatgagc tcctccttca tagatgccac cgatgatttt atttagtgtc
    41221 ccatcgccac caagagggat aaccaagtca atcttgtcct tagcagcttg ttttgctaat
    41281 tgaaaagcct ggtcagcacc tttaggggta ataacttcta cagaatcttc ggaaaaacca
    41341 tgttggcaaa aatagtcttt gacttgccca gcaagttggt cttctttctt tccagaatta
    41401 ggattgtaaa aaatccgaac ggttttcatc aggtctctcc tttgacagct tattacttgc
    41461 tatccattat accacttcta agaattgatg tcttacacca actcatcaca ttaagcctct
    41521 caatcagtct gagcatctat ttatcttaaa gcataaagaa agtgatagcg ataaattgga
    41581 gggcagaggc caataaaata aagagatgcc aaatcatgtg gaagtagggt ctttttttgg
    41641 cataaaatac cgctccaatg gtataagaca agccaccaaa taacatgaga agaccaaaag
    41701 caagactagt tttttgaatg atgactggca agataaacac aacgagccag cccataacga
    41761 tataaagcat caaactgaat ttttcattga tacgtttggc aaaaattttg tataaaatcc
    41821 caaaaagcgt aatgccccac tgcaaaacaa taatgatata gcctagccaa ccagaaacaa
    41881 gggataaggc aacaggtgta taacttcctg cgatagcaat gtaaatcata ctgtgatcaa
    41941 taatgcgtag aatgtatttg tgaaccgaac cataagccat ggagtggtaa atcgtagagg
    42001 acaaaaacat caaaaagaga ctgatgacaa agatggagat accaatagcg gctttaaggt
    42061 cataagtttg ataagcatag ctggctgaaa taggaagtag aatgagcata gcaaatgctc
    42121 cgatagcgtg tgtcacgctg tttgctactt cctcactaaa agataagggt aaactttgtt
    42181 taaaggtttg gttcattggg tcacctcctg tgataaataa atagttttgt ggtaatcttt
    42241 gaccgtttga caggcaacct gcacaatcgg tggaatgtcc tcctcaatgg gagctttctg
    42301 attccaacag gttacaaaag tattgtagag actttcagaa ccagcctgtg cctgaagttc
    42361 ctccaatacg cgactaatat cttgaatagg aaataccgtt ttaaacaaac tgatggcaat
    42421 taagcgcgct agttgttgct tttggtattt tttcttgata ggtttagtca catacccatg
    42481 cttgacgtaa ttattaatca tagatgccgt tagtgattta ttatctgaca cttctgaaaa
    42541 atcagtgcac tggttgacat aaagtaacac ttgatccaaa tataagtcta aatcagggag
    42601 gtccttccaa taaggtaatg atggaaaaat catagtttcg tctctctatc tagttttgat
    42661 aactagatta tattattaat gaaaacttat tgcaagcaaa aactgtaaag acttaaaaga
    42721 ggaaagtaga aatggcgaag tgaagctaat catgctataa tgttaaagaa ttgattggga
    42781 tagatgcctt ctgtcgcaat cagactattt tatcacgaag aagatgatgc tacgaaagga
    42841 atgatacact catggcaatg attcaatggt ttcctgggca catgtccaaa gccagaagac
    42901 aggttcagga aaatgtaaag catgttgact ttgtaactat tttagtggat gcacgcttac
    42961 cactttctag tcaaaatccc atgcttacaa aaattgttgg tgataaacct aaattaatga
    43021 ttttaaataa agctgacttg gcagatgcaa ctcgaactaa agagtggaag gcttattatg
    43081 aaagtcaagg tattaaaaca ttagccatca attccaaaga acaatcaacg gttaaaaaag
    43141 taacagaagc agccaaggaa ctcatggctg ataaaattca acgcttgcga gagcggggca
    43201 ttcaaaaaga aaccttgcga accatgatta ttggcattcc aaatgctggt aaatctacct
    43261 taatgaatcg cttggctggt aagaaaattg ctgtagttgg caataaacct ggtgtgacta
    43321 aaggacaaca atggttgaag tccaataagg aattggaaat tctagacact cctggtattt
    43381 tatggccaaa atttgaggac gaattggtgg gcttaaaatt agctttaaca ggtgctatca
    43441 aggaccaatt actaccaatg gatgaagtga ctatctttgg cttgaattac tttagggaat
    43501 actaccctaa tcgtttaacc aaacgtttta agaatattcc tcttgaggaa gaagctcctg
    43561 aaatcattat gaccttgaca cgccaacttg gatttaaaga cgattatgac cgtttttaca
    43621 ctttattcgt taaggaagtt cgtgatggta aattaggtca atacacctta gatcaggtgg
    43681 gaaatatgga tgccgactag tattaaagct attaaagaaa gcttagaggc cgttactagc
    43741 ctcttggacc ccctctttca agaattggca accgacacta ggtcaggcgt ccaaaaagct
    43801 ctaaaaagcc gacaaaaggc tattcaggcc gagttagcag aagaagaacg attagaagcc
    43861 atgctttctt atgaaaaagc tctttataaa aaaggttatc aagccattgc aggtattgat
    43921 gaggtgggac gtggtccctt agcaggtccc gttgtggcag cttgtgtgat tttacctaag
    43981 tattgtaaaa ttaaaggcct taatgattct aaaaaaatcc ctaaagctaa gcatgagacc
    44041 atttatcagg cagtgaaaga aaaggctttg gctatcggta tcggtattat tgacaatcag
    44101 ctgattgatg aggtcaatat ttatgaagca accaaactag ctatgctaga agccattaaa
    44161 cagttggagg gccaactcac acaaccagat tatctcttga ttgatgccat gacattggat
    44221 attgctattt cgcagcagtc tattcttaaa ggcgatgcca attccttgtc tattgcagca
    44281 gcatcaattg tagctaaggt caccagagat cagatgatgg ctaactatga tcgcattttt
    44341 cctggttatg gcttcgctaa aaatgcaggc tatggcacca aagaacattt acagggatta
    44401 aaagcttacg gcataacgcc tatccatcgt aaaagttttg aacctgttaa atccatgtgc
    44461 tgcgattcaa ctaatcctta aaaagttctg tttttagcag aatttttttc ttttttacga
    44521 ataaatgaag taaggaggta acttagtgaa tcattttgaa ctttataagc taaagaaagc
    44581 tggactgact aataaaaaca ttctcaatat tcttgactac caaaaacaac aggaaaaatc
    44641 gttgtcactt cgagatatgg ccgttgtttc tggctgtaag catccgtctc actttataga
    44701 agcctataag cagttagata ttcaaaaatt aaaaatagaa tttaaacaat ttcctagtat
    44761 ttctatctta gataagcatt acccaatggc attgaaagaa atatacaatc cacctgtcct
    44821 cttgtttttt cagggaaatt tagaccttct tgagaaacct aaattagcca ttgtcggctc
    44881 cagacgctca agcgataccg gagtaaagtc tgtccgtaaa attcttaaag aactcgggaa
    44941 ccgttttgtg attgttagcg gacttgctcg aggtatcgac actagtgctc atttagcctg
    45001 ccttaaaaat gggggacaaa ccatcgctat tattggaaca gggttggatc gcttttaccc
    45061 taaagaaaat agggagttgc aaactttctt agggaaaaat catcttgtga taacagaata
    45121 cggtccagga gaagaagctt tatcttatca ctttccagaa cggaatcgta ttatcgcagg
    45181 ccttagccga ggtattcttg tcgttgaagc aaaaaatcgt tcaggttcct tgattacttg
    45241 tcaaattggg atagaagaag gccgagacat ttttgctgtc ccaggaaaca ttttggacgg
    45301 gaaatccgaa ggttgccttc agttaattaa agagggagca acatgcgtca catcgggcat
    45361 ggatatcctt tcagagtact aaaaaagtta aagtttcttc ctatatagca tttttttatt
    45421 gacagtttct taaaaatggg atattatgct ataagcttta aacaaactgt tataggaagt
    45481 gtgtcgaatt gggaacaaaa actacaacta agccaaaaac gggtactaaa aagtccacca
    45541 ctaaaaagaa atcaacagcg aaaaaaaatc tcgttattgt tgaatcacct gccaaagcta
    45601 agactattga aaaatatctt ggacgcagtt ataaagttgt tgcttcggta ggacatattc
    45661 gtgacttaaa aaaatcgtct atgtctattg attttgataa taattatgag ccacaataca
    45721 ttaatatccg tggtaaggga ccccttatca attctttaaa aaaagaagcc aaagctgcta
    45781 aaaaagttta tttagctagt gacccggatc gtgagggaga ggctatttct tggcatttgt
    45841 cacatatctt aggacttgat cctcaagata ataaccgagt ggtttttaat gaaattacga
    45901 aagatgcagt taaacacgcc tttgtggagc ctcgtcagat tgatatggac ttggtagatt
    45961 ctcaacaagc acgtcgtgtt ttggaccgta ttgtgggata ttccatttca ccaattcttt
    46021 ggaaaaaggt caaaaaagga ctttcagcag gacgtgtcca gtcggttgct ttaaaactca
    46081 tcattgaccg tgaaaatgac atcaaagcat ttgttccaaa ggaatactgg tctattgatg
    46141 gtctttttaa aaaaggaact aaaaagtttc aagcgacttt ttacggtatt aatggcaaaa
    46201 aaacaaagct tgataataac aacgatgtca aagaggtatt agcaaagcta acaaatgaag
    46261 attttcttgt cagcaaggtt gacaagaaag agcgacgtcg taatgctccc ttaccttaca
    46321 caacatcatc tcttcaacaa gatgctgcta ataaaatcaa tttcagaacg cgtaaaacca
    46381 tgatggtagc ccaacaactg tatgagggga ttcatttagg agaaaatgga actcaaggtt
    46441 taattaccta tatgcgtacg gattctactc gtatcagtcc agtagcacaa aacgatgctg
    46501 ctcaatttat tatcaatcgc tttggagcta attattcaaa acatggtaat cgtgtcaaaa
    46561 ataccagtgg tgcccaagat gctcacgagg cgattcgtcc atctagtgtt aatcatacgc
    46621 cagactctat tgccaaatac cttaacaagg atcagttaaa actttatacg cttatttgga
    46681 atcgttttgt ggctagccaa atgacagcag ctgtttttga taccgtaaag gtgaacttgg
    46741 agcaaaatgg cgtgattttt gttgccaatg gtagccaaat gaagtttgat ggttacatgg
    46801 ctgtttacaa tgattccgat aaaaacaaaa tgttacctga gatggcagaa ggagaaacgg
    46861 ttaaaaaaat atcgacctct ccagaacagc actttacgca gccaccagct cgttattctg
    46921 aagcaactct gattaaaacg ctagaagaaa atggtgttgg acgtccatca acttacgctc
    46981 caaccttaga agtcattcaa cgcagatact atgttaaatt gtctgctaag cgctttgagc
    47041 caactgagct tggggaaatt gtcaacaagc ttattgtcga atttttccca gatattgttg
    47101 atgttgcctt tactgccgag atggaaggta agctagatca agttgaaatc ggagaagaac
    47161 aatggcaaca tgttatcgat caattctatc aaccctttgt caaagaattg aataaggctg
    47221 agtctgaaat tgaaaagatt caaatcaaag atgaaccagc tggcttcgac tgtgatgttt
    47281 gtggtcaccc aatggtcatt aagttaggac gttttgggaa attttacgcg tgtagcaact
    47341 tcccagagtg ccgtaataca aaagccatca ctaaagaaat tggtgttact tgtccggttt
    47401 gtcataaagg acaagttatc gagcgtaaaa ccaaaaaaaa taggatattt tatggttgtg
    47461 atcagtatcc agattgtgag tttatctcgt gggatttacc tattggacgt gcttgcccta
    47521 agtcaggtga ctatcttatt gagaaaaaag ttcgtggtgg caaacaagta atgtgtagca
    47581 atgagacctg tgattacaag gaagaaaaaa ttaaataaaa aaagaattgg aactccttgt
    47641 cttgttccaa ttcttttttt atttacaaag gtatttttaa ctacaaaaat cgctagttcg
    47701 tttttaaaag gcttaaaaat tcctcaataa ctggactgaa actcgcatca tctcggtaag
    47761 caatcccaat agggtaaaaa ttatttttat cttgcaaagg aatccaaact aagttatcta
    47821 cctgattaag actcttaaaa tcactcggga ggatagtcaa ggtatttaaa tcttgcaaac
    47881 tatgaataag aacttcccaa tcattgtgtt taaagacaat atctggttca aaaccgagag
    47941 ctcggcattt tctaggaagc atttcgccta gcatgtagtg atcgtttaaa gaagcaatct
    48001 tatagccttt taaatcttta agttttaact gaggatgttg cgccaaaggg tgctgtttga
    48061 ggagaacgat actgacctta tagccttttg tggacgtttg tagtagctca atggtaatat
    48121 ccttttgaat acttagaaag gagaggagac caatatcgat ttttccttgg gacaataatt
    48181 cttataattt gcgagaacca tcctgtataa gcgataattc aacatttgag tgagtagcca
    48241 taaagattga aatttgtttc atgaattgta tagcaaacag tgatgttaat tcaacacgaa
    48301 tcatctcttt ttttcttgat ttaattgctg aatatccgca acaatttgat taaagtcatc
    48361 cacaaggtct ttgcctttgt gataaagaat atctccagct tcggttagta gtagctgtca
    48421 atcactttgt gtaaaaagcg tcgtgttcaa actaggactc tagcctttta attgattgag
    48481 aaagagtatg ttgcgttaca aacaaacttt tagcagcgtg ggaatgattt tttgtttcag
    48541 caactgcaat aaaataggtt aattatctaa tatccatagt agatccttca tgattggtca
    48601 gggaattgcg tctctacttg gtttacaacc tattttgatg tctctcctta ttgccatgat
    48661 tttctgcttc cttattgttt ctccaattgc aacggtagga attgctcttg cgattaactt
    48721 atctggtatt ggttcaggtg ccgcaagctt tggactttgc ttagcaggtt gggctgttaa
    48781 ttcaaaagga acttcacttg ctcacgtctt gggatcaccg aaaatttcaa tggccaacgt
    48841 tttatcaaaa ccaaaaatca tgttgctaat gttatgttct gcagctgtac ttggtgttat
    48901 tggtgctatc tttaatattc aaggaacacc tgctagtgca ggctttggaa ttagtggttt
    48961 gatcggacct atcaatgccc ttaatttagc taaaggcgga tggtctccag taaatattct
    49021 acttatcatt gtcatttttg taggggcacc aattgttctc aatatgatct ttaactacct
    49081 ttttatcaag gtgcttaaag tgattgatcc aatggattac aaacttgata tctaagaaag
    49141 gaacttttga tgaaattaaa attatataat gttagaggcg aagaagcagt cttagcaaaa
    49201 aaatgggctg atgacaatgg gattgagatt tccttgacgg agtctccctt aactcctgaa
    49261 acggttaaag aagctgaagg ctttgacggg attgccaatg cccaaattgg tcctttagat
    49321 gatgcgattt atcctctctt aaaggaaatg gggattaaac aaatcgctca gcgcagtgct
    49381 ggtgtcgata tgtataacct tgatttggcc accgaaaatg acattattat cacaaatgtt
    49441 cctagctatt ctccagaatc cattgcagaa tttacggtta ctattgccct taatttaatt
    49501 cgtcatgtgg agttgattcg tgaaaatgtt aaaaaacaaa atttcacttg gggactccct
    49561 atccgcggcc gtgttttagg tgatatgaca gttgcgatta tcggaactgg acgtattggt
    49621 ctagctactg ctaaaatctt taaaggtttt ggccgcaagg tcgttggata tgatatttac
    49681 caaagcgatg ctgccaaagc tgtcttggac tataaggaat ctgtagaaga agcgattaaa
    49741 gatgctgatc ttgtttcctt acacatgcca ccaactgcag agaacacaca ccttttcaat
    49801 tctgatttat ttaaatcatt caaaaaaggg gctattttga tgaatatggc gcgtggtgcc
    49861 gtcattgaga cgcaagattt actggatgct ttggatgcag gcttactgag cggagctggt
    49921 attgacactt acgaatttga aggaccttat atacctaaga attttgaagg tcaagaaatt
    49981 acagattcac tatttaaagc tttgattaac catcctaagg ttatttatac gcctcatgca
    50041 gcttattata ctgatgaagc cgttaaaaac ctagttgaag gtgcccttaa tgctacagta
    50101 gagattatca aaactgggac aacgacaact cgtgttaact aacaactgat ttgacgtcca
    50161 ttataaagga ataattttaa aaattccttt ataatggact tttttatatt agtccgttat
    50221 aatagactgt aagaaagggg actcttatga catatatagc gcttattgga gatattatcc
    50281 agtccaaaca attgacggat cgttccaaag ttcaaaaaac tttagcagct tatttagacg
    50341 acctcaacaa aacctttgct ccttatatta tttctaaatt atccctaacg ttgggagatg
    50401 aatttcaagg actttttcag gtggatactc ctattttcca tctgattgat ttgattaatc
    50461 atcacatgga tatccctatt cgatttggag taggagtagg ttctattcta acagacatca
    50521 atcctgatat tagtattggg gctgatggac ctgcttattg gcatgctcgt gaggctattc
    50581 gttatattca tcaaaaaaat gattatggta atactacatt agctcttcgg actggtcatc
    50641 acaaccagga tgatgtatta aacagtttac tagcagctgg agatgctatt aaagccaact
    50701 ggcgagctag tcaatgggaa atttttgaca cgcttttaga tttgggcatt tatgaggaat
    50761 actttgatca acaacgacta ggaaagcaat tatctcttag ctctagcgct ttgtcaaaac
    50821 gtctaaaaag tagtcatgtt aaaatttatc tgagaacacg gcagagtgcg ctaaactgtc
    50881 tcaaacaaat caaggaggaa gctgatgatt aatggtgtct ctcactacct agctcaaacc
    50941 cctacactta ccctgtttct catttgtcat gtgttatcag actaccagtt acaaagtcag
    51001 caagtagctg atttaaaaga aaaacatttg acttatttag gttaccactt gattggagtt
    51061 agcattcccc taatttgttt aaccctcatt atcccacaag catggttgat gagcctattg
    51121 gttatgataa gtcacgcgct tattgactgg ctaaagccaa agatggctaa ctctctaaaa
    51181 tggaaaagag agtggatttt tctgctggat caatgcttac atatcgctat tagttcattt
    51241 gctgggctaa ggttagcagg gatgaccttg cctaattggc ttccgatatc tattttgatg
    51301 accgttttat ttatcctact cattaccaaa ccaactaata ttgtctttaa actctttttc
    51361 agcaaatacc agcctgacca aggagaaaaa atggacacga tcataggagc ggggctacca
    51421 taggaattct agagagaatt gttattgggg tgtgtatgat catggggcaa tttgcttcaa
    51481 taggtctggt ctttaccgct aaatctattg ctcgctataa taaaatttcg gaaagtccag
    51541 cttttgcaga atactaccta attggttctt tatttagtat tctcagtgtt tttatcgcag
    51601 catggatttg tttcttctag ttgaaaaggg ttcccgtaga aagaggctac acatagcctc
    51661 ttttgctttt ttcccctttc tgttataata agtcaggtaa ctacattaga aagatttgtg
    51721 ggtctgtcat ctaagagatg tcattcccga agaggtatta gtgacttgtc tcaatcaact
    51781 gcaacttata ttaatgttat tggagctgga ctagctggtt ctgaagctgc ctatcagatt
    51841 gctaagcgcg gtatccccgt taaattgtat gaaatgcgtg gtgtcaaagc aacaccgcaa
    51901 cataaaacca ctaattttgc cgaattggtc tgttccaact catttcgtgg tgatagctta
    51961 accaatgcag tcggtcttct caaagaagaa atgcgtcgct tggattccat tattatgcgt
    52021 aatggtgaag ctaatcgtgt acctgctgga ggagcaatgg ctgttgaccg tgaggggtat
    52081 gcaaagagtg tcactgcaga gttggaaaat catcctctca ttgaggtcat tcgtgatgaa
    52141 atcacagaaa ttcctaacga tgccattacg gttatcgcga cgggaccgct gacttcggat
    52201 gccctggcag aaaaaattca cgcggtaaat ggtggcgatg gattctattt ctacgacgca
    52261 gcagcgccta tcattgataa atctactatt gatatgagca aggtttacct caaatctcgc
    52321 tacgataaag gcgaagctgc ttacctcaac tgccctatga ccaaggaaga attcatggct
    52381 ttccatgaag ctctgacaac cgcagaagaa gccccgctga atgcctttga aaaagaaaag
    52441 tattttgaag gctgtatgcc gattgaagtt atggctaaac gtggcattaa aaccatgctt
    52501 tatggaccta tgaaacccgt tggattggaa tatccagatg actatacagg tcctcgcgat
    52561 ggagaattta aaacgccata tgccgtcgtg caattgcgtc aagataatgc agctggaagc
    52621 ctttataata tcgttggttt ccaaacccat ctcaaatggg gtgagcaaaa acgcgttttc
    52681 caaatgattc cagggcttga aaatgctgag tttgtccgct acggcgtcat gcatcgcaat
    52741 tcctatatgg attcaccaaa tcttttaacg gaaaccttcc aatctcggag caatccaaac
    52801 cttttctttg caggtcagat gactggagtt gaaggttatg tcgaatcagc tgcttcaggt
    52861 ttagtagcag gaatcaatgc tgctcgtttg ttcaaaagag aagaagcact tatttttcct
    52921 cagacaacag ctattgggag tttgcctcat tatgtgactc atgccgacag taagcatttc
    52981 caaccaatga acgtcaactt tggcatcatc aaagagttag aaggcccacg cattcgtgac
    53041 aaaaaagaac gttatgaagc tattgctagt cgtgctttgg cagatttaga cacctgttta
    53101 gcgtcgcttt aacactaaaa aagagttcct tttcagttga gttgctgaag aggacttttt
    53161 tatttgctga tttgagcgct agctatgcaa aagaatgcac aaaaaataca tgaatagact
    53221 atggtcacta gaagcaatat tatataatga atagtactca ttagttgatt cagtttggag
    53281 gttttagcat gcagcagcaa gtcgcaataa cagaaacagt tttacgggac ggtcatcaaa
    53341 gtttgatggc aacgcgttta tctattgagg atatgcttcc tgtccttacc atcttagaca
    53401 agattggtta ttattcccta gagtgttggg gaggagcaac ctttgatgcc tgtattcgtt
    53461 ttttgaatga agacccttgg gaaaggctgc gcacgttaaa aaaaggctta cctaataccc
    53521 gtttacaaat gttattaaga ggccaaaacc ttttaggcta ccgtcattat gcagatgaca
    53581 tcgttgacaa attcattagt ttatcagctc aaaatgggat tgatgttttc cgcatttttg
    53641 atgccctaaa tgacccacga aatatccagc aagccttaag agctgtgaaa aagacgggga
    53701 aagaggctca actgtgtatt gcctatacaa ctagcccagt gcacacgctt aactattacc
    53761 tttctttagt gaaagaattg gttgagatgg gagctgattc tatctgcatc aaagatatgg
    53821 ctggtattct aactcccaaa gcggctaaag agttggtatc tggtatcaaa gccatgacta
    53881 acttacctct tattgtgcat acccatgcaa ctagtggcat ttctcagatg acctatctag
    53941 cagcagtaga agctggtgct gatagaattg ataccgcttt gtcacctttt agcgaaggca
    54001 cttcacaacc tgctacagaa tccatgtatt tagccttaaa agaagcaggt tatgatatca
    54061 cattagatga aacgctactg gaacaggctg ctaatcacct tcgacaagcc cgtcaaaagt
    54121 acttagcaga tggcattctg gatcccagtc ttctctttcc agacccaagg acactacaat
    54181 accaagttcc tggaggcatg ttatctaata tgctatctca gctgaaacaa gccaatgccg
    54241 aatcaaaatt agaagaagtt ttagcagaag ttcctcgggt aagaaaagac ttgggttatc
    54301 ctcccttagt aacaccgctt agccaaatgg tcgggactca agctgctatg aatgtgattt
    54361 taggaaaacc ttaccagatg gtgtctaagg aaattaaaca atacttggca ggcgactatg
    54421 gcaaaacacc agcaccagtc aacgaagact taaaacgaag tcaaattggc tcagctcctg
    54481 tcaccacaaa tcgtcctgca gatcaactaa gtcctgaatt cgaagtgtta aaagcagaag
    54541 tagcagattt agcccaaacg gacgaagatg tgctgactta tgccctcttc ccaagcgtag
    54601 caaaaccgtt tttaacaacc aaataccaaa ctgatgacgt cattaaagtg actgccttta
    54661 tcaaagcata ataggctatc ttaatcagat agaaacaaaa atatggagat caaatgacct
    54721 attcctttat tgatatctta caagttacct tagtatcctt gttcttagtg ttaacctgtt
    54781 taacagggat tatgggatta atgatgctaa gtggtaaatt attgtcacaa gaaaaaaaaa
    54841 ctctctctca acaagcttcc agcattcctc aagagcaaga agctgacaag ttgagtggag
    54901 aagctctcga aactctttta gccaaagacg aattggctag aattgctgtt atgactagtc
    54961 ttgcttatgc tagccaagaa gagtcaggaa aacggtttga agttattgcc gtggaaagga
    55021 aatcctaatg aaaagatatg aagtgactgt caatggacag gtttatgaag tgagtttacg
    55081 tgaactagct gatggagaaa ctgttgaagt tagccagcca gcagcacccg ctactgagaa
    55141 agaaatgaat gccaacgctg ctggcggtgg cattcaggta aagactccaa tgtctggaac
    55201 agtcttgtct atctttgcta cagaaggaaa agctgttaaa aaaggagaag ctgttctggt
    55261 cttagaggcc atgaaaatgg agaatgaaat tctagcacct gcagatggtc tggtttcaaa
    55321 aattcatgtt gtggctaacc aaacggtcga gtctgaacaa gttttgattt cattctagga
    55381 ggaaaccatg ctagacgtac tcaatcaaat ggtccaatcg tcaggattgg ctcatctaac
    55441 tgttaataat cttatcatga tttgcttggc ctgtttcttt ttataccttg gtatcaaaaa
    55501 ggaatatgag ccttacctta tggtaccaat cgcctttggg attttactcg ttaatttgcc
    55561 tatggcggga ttgatggacc accctgccaa tggtaatcct ggtggccttc tttattacct
    55621 atataaggga actagcctag ggatttaccc acctttaatt tttctttgtt tgggggcatc
    55681 aactgacttt ggtcccttga ttgccaatcc taaaaccatt ttactaggag gcgcagcaca
    55741 agttgggatt ttcttagcct ttttccttgc tattatgtta ggcatgacac ctcaagaagc
    55801 ggcttctgtc gggattattg gtggtgccga tggaccaaca gctatttatg tgacgacaaa
    55861 gttggcgcct gatttactct ctactatcgc cctagcagct tattcttaca tggctctggt
    55921 acctattatt caacctccta tcattaaact cttgactacc aaggcggaac gccaagtgaa
    55981 aatgactcaa gcaagaactg tttcacaaaa agaaaaaatc attttcccga ttatggtaac
    56041 aatctttgtc agccttttgg ttccaagtgc tacaaccttg gttggctgtt tgatgctggg
    56101 aaatctggtc agagaaatta aaattgttcc taaaattgta gagaatctcc aacaagtggt
    56161 tatgttttgc attactatca ttttagggtt aaccgttggt gctaaggcca atggagatct
    56221 ctttttatca gccacaacct tgaaaattat tgcccttggg ctaatcgcct ttgctgctgg
    56281 tacagcaggg ggtgtcttga tggggaaggt catgtactat ttatctggta acaaagtgaa
    56341 ccctatgata ggagcagcag gtgtttcagc tgttccgatg gcagcgcgag tggtccaaaa
    56401 ggtcggacaa gaagaagatc caagtaattt cttactcatg cacgccatgg ggcccaatgt
    56461 agctggcgtt attggctcag ccattgcctc aggtgccttg ctggccttct ttggctaatt
    56521 gtgaaaaagg attagtaaat taatcttaat aaatcattat caggagttgg aagcaatcta
    56581 gctccttttt tagtagaaag ctcgagtgat aaactattcc aaaataatta taaaaaaaga
    56641 ctgtcctgat tgtacggact atatcaattg acttttatag tctagctgaa atagcagtgc
    56701 aaaactaaga cactcgtttt aaagtagttt ttctaaaaaa gcaaaataaa aggtcagagc
    56761 caataaatct gccgcgcccc cagggcttaa atgctgatta atgaggcatt gattgtagga
    56821 ggacaaagcc aatcttaggt cagttgttga taaatcttgt tgcaggagta gtcgcatatc
    56881 tgctttaacg cctttccatg cttcaatccc tccacgatga atcagatttc catcttcaac
    56941 aatagacatc aaataaacaa gaagacgaag ctgactggtt tcggggtcat tttgacttat
    57001 catctgacga aagtagggca aggcatggtc tgttaaggtc gtgaatccct cgcttgcctc
    57061 accccgaggt cctttgatgc cataagttac aaagagttgc tccccataag tgaactcttt
    57121 tttagtgttc aaatgaccaa ggtctgtttg aatcaaatgt gccgtcatag gttttactaa
    57181 acgacagata gctagagtat cttccttgct aaaacaccct aaatcagtca ttaagtgagg
    57241 tgttcttgct agataggccc cagttgcacc aagaagtaaa gccatggaaa aattcaggcc
    57301 tttatgagtg ttaattccct gagtagcaca aaacatggtt tcctcggcct tttgacctag
    57361 ttgtctaaga cggttaaaca gaagaagtgg ctcttctttt gcataagcaa aacctgtttc
    57421 gatataagcc tgaaaaaaag gagagagtgc tatcatactg tcgataaagg tcataaagga
    57481 catgtcatca tgggcaccgt tatcaaatcg atcaactaaa ccaggttttg gagatagtga
    57541 gacttcataa agcaaagctt ttaaggccag ttgactaata cttgttagaa ctgccttagt
    57601 cattttgctc aggcctcatt tcttttaaga taaattcaag tgagctattc aagtgttcat
    57661 gtgtaatcaa cgtaatttgc tcatgatctt tgttcttcat acctcggtaa atcatccagt
    57721 gttcagatag agctcgttta cggcgttcaa ctgaagaaat cgaaattttt ctaaagtaga
    57781 ccaaataagc ttgcaattct gtcacaattt cttttaagcg gaccatttgg ctatagctgt
    57841 aaatcaaatt gttaaaggca ttaaaattat ccaaaatacg attggtgtca tcctctgcga
    57901 taaaggtttc acagtcttcc aacaagttat gcataacttt aaaatcttct tctgtcatta
    57961 aatgcatcgc ttgcactgtt gcgagagtct ctagagcttt tcgaatttca aaaatttcac
    58021 aggcatcctt gatactaact cctttgacaa taatgccttt tttaggaatg tgctctacaa
    58081 ggtgttcttc agacaataat cccaaagcgt agcgaatagg tgtcctgcta atattaagcg
    58141 caatggaaaa ctctttttca ttaatgcggc ttccaacagg aatttgtcgc aaaataatgg
    58201 tctttttaag agcgttataa aaggctattt tcaaagggat atttcttgat aggtcaagat
    58261 tttgtttaac ggcttcgata ataggattca tgtcatctcc ttaatgctag tgtcctcact
    58321 ttattatact taaatttgtg aaacattcca ataggactag atgagttctg aaaaattaaa
    58381 gccatgctca caaaaggaca tgactttaat catctcataa ggaataaggt agtagtgtta
    58441 tcgttggttt attgtggaat gtaaagtggc atttgcccca tggcaataat ggttaccaca
    58501 aagataacaa agatgataag ggcatattta gcagcctctt tttgccattc ccccatgtca
    58561 agacctgtca agcgaagaag aagataaatg aaggcaacta atggactaag taagtggaag
    58621 gcttgtccca tgagggaagc aagtgccata gccatgttac tgaaaccata ttcagcacca
    58681 gcttctgcaa gaacaggcaa gataccatag taaaaaccat cattggagat aaagaaggta
    58741 ccaggtgcag aaactaaggc aataacaaga ccccagaaac ctgccagttg ttttggaata
    58801 atggttgcaa aactttgtgc aagagcgcta gccataccag aaccttgaaa aagacccata
    58861 aagataccag cagcaaagac gagaataacc acttgaaccg catcaccagc attgtcacca
    58921 atgcgttttg attgatcttt aagtactggg taattgacca tcaaggcgat acaagttccc
    58981 accgcaaaaa gaagaagcgg tgccatagca atagatttgt tgaaagagcc agcaactaac
    59041 caagtgatta atccaatggt taggatagca ttgaaaacaa agtttttagg acgacgaata
    59101 aaaagggtat ctgggtcagt aatgtctgtg agttgacgca tgtcttcttc agacaagtca
    59161 ataacaccaa gtcttgcacg ttctttacgc cccatgcttg gagcaaccca acaaatcaca
    59221 taaagaagag ataaaatcat acccggtgca agatagccaa gaatttcagg accgacacca
    59281 agaacagaca tcgcacgagc agtagggcca ccccatggca gtaagttcat aatagtattt
    59341 tgaagaataa tcaagacacc taggttcatg attttcatgt ccaatttttt atagataggt
    59401 aagaaagcag agcaacaaat taaagtagtg gttgttccat caccattaag agatactgca
    59461 gcagcaacga cagctgttgc gataagcacc ttcattggat cacctttagc aaagcgaatc
    59521 atcttttcag taatcggatc aaacaatcct gcatctaaca tgacagagaa gaacaaaatg
    59581 gcaaatagca acatgatacc tgttttagca gtattgttga taccatacat aaccattggt
    59641 ccgatagctg ttaggtcttt agcaattcca ccgtcaccaa caaaggcaac aaatttggca
    59701 tctgcattga agtcagctga accagtcagt ataacagcaa tcgtcatgat taatggaatc
    59761 atgaccaaag cagtaaaagg ggtcattttt ttcttcatta ccacgtacat aaagacaata
    59821 atcatggcat aagccagcat tgttaataac atcattgtta tctccttttt caagttatga
    59881 tttcagtata gatgaaagcg cttataaaat aaatacttct ttgtgcagaa agacacaaaa
    59941 aaaacaattt gaaaattaat tttgaaaacc atttttatta catcagatga ctttaaattt
    60001 tctataataa tgatgaacct agttagatca agcttttctt gctatcagta tcttgatctc
    60061 agtttttcaa cagttaccta aaaataaaat aaaaggtaac tgttgaaaaa ctttatgaaa
    60121 cattgaaagc gctcttagaa agaacacaaa aaagccatct caaaaaagat tggtagtata
    60181 taataaactt actagttccc taaataggta aatgtgaaaa agaggtagct catgcagttt
    60241 ttacaaaaat ggtttaaaca ctctaaaaaa gagaagttga aagaatcctc gccccttagt
    60301 acagaaatag aaccttctga aaactgggaa aagattcccg cttatattcc tgcagacaaa
    60361 agtgattaca aaaaggttac tttaattacc agtgctattg ctgctggcga tagacctaat
    60421 agtcaattca aggttaaacg cattctcaag cgcaattctg aggcaatcac tgtttcttta
    60481 attgcttcaa gtatcgctgc aggtgtttac ccagaaagtc agtttcgggt gacatccatc
    60541 tattgcaaaa ggtagtcggt gaacgatact ttagacgaac tcatgacaca ataacatata
    60601 ggagaaaaat gatgttacga aaatttaaaa ttaccattga cggtaaagaa tatcttgtag
    60661 aaatggaaga aatcggcgct cctgcccaag cagctgcccc cgctcaacca atatccactc
    60721 cagtacctgt ccctactgaa gcaagcccac aagtggggga agcgcaagca ccacagccag
    60781 tagctgcagc gggagcggat gccattccat caccaatgcc tggaaccatc ttaaaagtct
    60841 tagtagcagt gggagaccaa gtaactgaaa atcagccact attgatttta gaagccatga
    60901 agatggaaaa tgagattgtg gcctcatcag caggaaccat tacagctatc cacgtcggtc
    60961 caggtcaagt ggtcaatcct ggtgatggtc tcattacaat tggttaatag tactataact
    61021 agaataaata gacagaaaag gaagtgaatt tgtggacact ttaatcgcag gaataacctc
    61081 catcaccatt cctcaaattg ttatgatggt catcggtgcc ctcttaatgt atctaggaat
    61141 taaaaaagaa tacgaaccaa ctctacttgt ccccatggga cttggaacca ttctcgttaa
    61201 cttccctggt tcaggagttt taacccaagt ggttaacgga gtcgagcaag aaggggtttt
    61261 tgaggctctc ttcaattttg ggattgaaac agaacttttc ccactgttga ttttcattgg
    61321 tattggcgcc atgattgact ttggtccctt gcttcaaaac ccattcatgc tcttgtttgg
    61381 tgctgcagcg caatttggga ttttctttgt tgttgttgtt gctgtactgg ctggctttga
    61441 cattaaagaa gcagcttcaa tcggcattat cggagcagca gatggaccga cttctatctt
    61501 tgttgccaac caactggcca aggatttact gggtccaatt acggttgcag cctattctta
    61561 catggctctg gttcctatta tccaaccatt tgccattaaa ttagtgacaa cgaaaaaaga
    61621 gcgccgtatc cgtatgactt acaaagctga gaatgtatct caaatgacta aaatcttatt
    61681 cccaatcatc attaccttag tagcagggtt catcgcccca atttctctac ctttagttgg
    61741 ctttttgatg ttcggtaact tattacgtga gtgcggtgtg ctcgatcgcc tgtcacaaac
    61801 tgctcaaaat gaattggtaa acattattag tatcttgctt ggattgacca tctctattaa
    61861 aatgcaggca gacctgttct taaatgtaca gacactctta attatcgtct ttggtttact
    61921 tgcttttatc atggattcta tcggtggggt catgtttgcc aaatttctca atctcttccg
    61981 aaaagagaag attaatccaa tgattggcgc tgcgggtatt tcagcttttc caatgtcaag
    62041 tcgtgtcatt caaaaaatgg caacagatga agacccgcaa aactttatcc tcatgtatgc
    62101 tgtaggtgcc aatgtctctg gccaaatcgc ctctgttatc gcaggtggtt tgttgctagc
    62161 ttactttagc taagaaagga aagaaaccta atgacaatta atatagacca tttgattagt
    62221 tcctttgagc taatggcgct tggtatggca ggtgtcttta tcgtgttagg cattctctat
    62281 cttgtcgcag agatcctgat caaacttttc ccagttagta aataataata tcatttaaga
    62341 aaatcaagga ggtaaacgca atggatatta aacaaactgc cgttgctggg tcacttgaat
    62401 caagtgacct aatgattaca gtatccccta atgacgagca aaccattaca ataaccctag
    62461 acagtagtgt tgaaaaacaa tttggcaatc acattcgtca actcattcat caaaccctag
    62521 tgaatttgaa ggtgacggct gccaaagtag aggctgttga taaaggtgct ttagattgca
    62581 ccattcaagc gagaaccatc gcagctgttc atcgcgctgc tggtattgac caatatgatt
    62641 ggaaggagat tgactcatgg aacgtttaag aagaacaatg atgtttgtgc ctggtgccaa
    62701 tgcagccatg cttcgagatg ctcctttatt tggcgctgat tccgtcatgt ttgacttgga
    62761 agattctgtt tcactcaaag aaaaagacac ctctagagct ctcgtccatt ttgcgcttaa
    62821 aacttttgat tattcaagcg ttgaaacggt tgttcgtgtg aatggtcttg attcttgtgg
    62881 ggctttagac atcgaagctg ttgttttggc aggtgtcaac gtgattcgtc ttccaaaaac
    62941 agagactgcc caagatattg ttgatgtgga agctgttatt gaacgtgtcg aacgcgaaaa
    63001 cggcattgaa gttggccgca cacgcatgat ggcagctatt gagtcagccg aaggtgttct
    63061 aaatgctcgt gacatttcca aagcttctaa gcgcttgatt ggtattgctc ttggagcaga
    63121 agactatgtc acgaatatga aaacgcgtcg ttacccagat ggtcaagaat tattctttgc
    63181 tcgtagcatg attttacacg ctgctcgtgc tgctggaatt gctgccattg atactgttta
    63241 ttctgatgtc aataataccg aagggttcca aaacgaagtt cgcatgatca aacagttagg
    63301 gtttgacggt aaatcagtaa tcaaccctcg tcaaattcct ctggtcaatg agatttatac
    63361 cccaacaaaa aaagaaattg accatgccaa acaagtcatc tgggcaattc gtgaagctga
    63421 aagcaaaggc tctggcgtta tttccttaaa tggaaaaatg gttgataaac caatcgttga
    63481 acgtgcagaa cgtgtcattg ccctagcaac agcagcaggt gttttatctg aggaggacat
    63541 ttaaagatgg taacaaataa actcggtcgc gacattcctc aaccatacgc cgaccagtat
    63601 ggtgtctttg aaggagaact cgcaaatatc aaacagtatg acgaatcaag tcgccgtatt
    63661 aaaccagtta aacctggaga ttctaaatta ttaggctcta tccgtgaagc gattgaaaaa
    63721 actggcttaa cagatggtat gaccatttca ttccaccacc atttccgtga aggggatttc
    63781 attatgaata tggtcttgga ggaaattgcc aaaatgggca ttaaaaacct atctattgct
    63841 ccaagttcca tcgctaatgt acatgagcct ttgattgatc acatcaaaaa tggtgtggtt
    63901 accaacatca cttcatctgg tcttcgtgac aaagtagggg cagccatttc agaaggacta
    63961 atggaaaatc ctgtggttat tcgctcccat ggtggtcgcg ctcgtgccat tgctagtggg
    64021 gatatccata ttgatgttgc ctttctgggc gccccaagtt cagatgctta cggaaatgtt
    64081 aatgggacaa aagggaaagc aacctgtggt tctttgggct atgccatgat tgatgccaaa
    64141 tatgcagatc aggtggttat cttgacagat aatttggttc cttatcctaa taccccaatc
    64201 agcattcctc aaacagatgt tgactatgtg gtaacagtgg atgctatcgg agatcctcaa
    64261 ggaatcgcca aaggggcaac tcgttttacg aaaaatccta aagaactctt aattgcagaa
    64321 tacgcggcta aggtcatcac taattctcct tatttcaaag aaggattctc tttccaaact
    64381 ggaacaggtg gcgcttcttt ggcagtaacc cgtttcatgc gtgaagctat gattaaagaa
    64441 aatatcaaag ctagctttgc tcttggtggt attaccaatg ctatggtgga gttgctcgaa
    64501 gaaggactgg ttgaaaaaat tcttgatgtc caagactttg atcacccatc agctgtttct
    64561 cttggtaagc atgctgaaca ttatgaaatt gacgccaaca tgtatgcctc acctttgagc
    64621 aagggtgctg ttatcaatca attagacact tgtattttat cagcccttga agttgatact
    64681 aacttcaatg ttaatgtgat gacaggatct gacggcgtga ttcgtggcgc ttctggagga
    64741 cactgcgaca ctgcctttgc ggctaagatg agtttggtta tttcaccact tatccgcgga
    64801 cgcatcccaa cttttgtaga tgaggttaat acggtcatta caccaggaac aagtgtcgat
    64861 gtggtcgtca cagaagtggg aattgccatt aacccaaatc gtcaagactt agcagaccat
    64921 ttcaaatccc tcaatgtgcc acaatttagt attgaggagc taaaagagaa ggcttacgct
    64981 atcgttggca cacctgagcg tattcaatat ggtgataagg ttgttgctct tattgaatac
    65041 cgtgatggta gcctcatgga tgtggtttac aatgtgtaaa gacacttatt tttcaggcga
    65101 agctatccaa cttagtgata tgttaagagc ccgcgaagaa agagctctgc gtcagctgca
    65161 tttattaaag gagtacccag aaggtagctt attatcggtc accatgaata tccctggacc
    65221 aattaaaacc tctcctaaac ttcttgaagc ttttgatata gtgattaagg ccattcaaac
    65281 tgccttagct gacgataaga tttgttacca gttgcgatta ctgcctacaa cgggttatga
    65341 gtattacctc atcacaagtc tacctagccg cgacctgaag ttaaaaatga tagccttaga
    65401 gacagagttg ccaataggtc gtctcatgga cttagatgtc ttggtcttgc aaaatgatct
    65461 gcctcattca attagcagaa ccgtattagg aggctcccct aggcaatgtt ttatctgttc
    65521 taaagaggcc aaagtctgcg gtcgcctacg taagcacagt gtcgaggaga tgcagactgc
    65581 tatttcaaaa ttactccatt catttttcaa taaagacaac caatcatcgt catcagataa
    65641 gacaggttga tgaggagaac aattatgacg aaaatccgta taacggaaac agttttacgt
    65701 gatggtcaac aaagtcagat tgccacacgt atgacaacca aggaaatgat tcctattcta
    65761 gaaactctag acaatgctgg ttatcatgcc cttgaaatgt ggggaggagc aacctttgat
    65821 tcctgcctac gctttttaaa tgaagaccct tgggaacggc taagagccat ccgtaaagca
    65881 gtgaaaaaga ctaaacttca gatgctctta cgcggacaaa accttcttgg atatcgcaat
    65941 tatgctgacg atgtggtcag atcctttatt caaaaatcca ttgaaaacgg gattgatatt
    66001 gtccgtatct tcgatgcttt aaacgactca cgcaacttgc aaacagctgt ttcagcgact
    66061 aaaaaatttg gagggcatgc tcaagttgcc atttcttaca cgacaagtcc ggtacatacc
    66121 attgactact ttgttgaatt ggcgaaagct taccaagcta tcggagctga ctccatttgt
    66181 atcaaagaca tggctggtgt cttaactcct gaaataggtt accaattggt caaatgtatc
    66241 aaggagaata caactatccc tcttgaggtt catacccatg ctaccagtgg tatttcagaa
    66301 atgacttact taaaagtagc agaagcagga gctgatatta ttgatacggc gatttcctct
    66361 ttttcagggg gaaccagtca gcctgccaca gagtcaatgg cgattgcctt gacggatttg
    66421 ggcttcgaca ctggcttgga tatgcaagag gtggccaaag ttgcagaata tttcaacact
    66481 attcgtgacc actatcgaga aatagggatt ttaaatccta aagtcaaaga cactgagcca
    66541 aaaacattga tttaccaagt tccaggtggt atgctatcaa acctattgag ccaattaacc
    66601 gagcaaggcc tagctgataa atacgaagaa gtcttagcag aagtacctaa agtaagggcc
    66661 gatcttggat atccaccact tgtaacgcca ttatcacaaa tggttggcac acaggccttg
    66721 atgaatatca tctcagggga acgctacaag gtagttccaa atgaaatcaa agactatgtt
    66781 cgagggctat acggtcaatc accagcccca ttagcagagg atatcaaaga gaaaatcatt
    66841 ggtgacgaag cagttattac ttgcagacca gccgacctaa tcgagcctca aatgatttat
    66901 ctacgtgacg agattgctcc atacgctcgt tcagaagaag acgtgttaag ctatgcgagt
    66961 ttcccacagc aagccagaga tttcttggga cgccgtgaag atcctttcta tgatgttccg
    67021 gttcaagaag ttactgtaca actggacatt caagactaac agggaatgac taatcctctt
    67081 aaccattaat ctccttggtg aaacgcattt gctgtaagaa caataccaaa aacctacctg
    67141 attttgataa tcagataggt tttttatctt agtaatgttt gacagatgaa gtggcttgta
    67201 tctttttaat aatgggttgg gcttctagcg aacaaaaata atctaaagac gttttgggaa
    67261 ggagttttgc cagaagttcc caatcattat ctttcaatgc ctgcctagcc gttgaggcac
    67321 tgataggatc ttgctgctga ttgatagctt tccttggcaa aatatctagg aggataccag
    67381 cttgctgcaa ctgctctgcc ataatttcat tgtaaatagc agttactcgg ctggttggct
    67441 cttctccaac gtatcttttt gtaatctgta aggtttgagc aatctttaga aaaatagcgg
    67501 tatccagcaa agcttggctc ttaatcacgt cattgtcttc tttttggaaa taggctggga
    67561 aagtcgcttg gctaatcaaa taaggacctg tttcatgata tatgacatta tcaagatgag
    67621 ctagaccttc ttgaatcaac ctctttctca ccgaaaaagg aattagactg cggtcttcac
    67681 tgaccataaa cagatgaacc caatcatttt ctcttgcagc tttctctact aaaaattgat
    67741 gccctaaggt aaaggggtta gcgttgataa cgacagcagc gactttacca ggtgtttgct
    67801 caggcttatt aagtgtcatc agataatctt gaaaaccagt ttttttattt tccaagaaac
    67861 tgatgtgatt gtcgatatgg acaatttcag taaaaccaag atctttgaaa aaagcagcag
    67921 aagaggtctt cgtataaaca aaaagatgat agttgccaag ggtatattct tcatcaatga
    67981 gatgactcac aatcctatta agaagtcctt caccttgata ggcactggat acgcaaagac
    68041 agcgcaagct atttccaaag taagaaccgg tagcaatcac atcattttgc gcattcatga
    68101 tggcacaagt ataatctaaa tgagcatcca aacgaatacc ttcttgatga agtaggttct
    68161 ttacactagc cattgtcgtt ttatctgaag gaaagacttt tgaaatagtg tagtaaggca
    68221 tttcttctcc ttatctttaa ttttattgaa tcataacata acttagtacc aaaacaggct
    68281 gatctatcta aaggcaactc tgttttttat caacgccatt ttttaaaagg aaatcgaatg
    68341 tgtttgacag actcaaaagt atcagcgatt ttttgattgc gttccacagt ccgcaagttt
    68401 tcttggtcta ataagtcttg acttttttcc aaaacatcaa cagtaatgta gtagagtttg
    68461 atcaggcttc ctatcttata tttaccagat tttggagaca tagcaaccac ttcattgaga
    68521 ttactatgaa gccatttttt atgaggctta gcaggtatag tggctactac aaagccaagt
    68581 tgttccaaat gagcttgtgc ctgatcaaca ggaagattaa tcacatcatc tatcgtgcgt
    68641 agttgcatct ctttttcatg tttttgttcc atacgttttt cgataattgg tcgactgtta
    68701 tcaatcgttt ttccaataat ttcagtagtg tcaggaacaa ttgacatcag tttcccaact
    68761 actcctagag cttttcttat tctctttggc atcttttatc tcctcaagtc gtttcttttt
    68821 attataacaa aatgtctaaa aatggtatgc gataaacctt gttctaaaag gttaaataga
    68881 tcaattcaag gaaaaaatag gaggggatcc gacaaaaaat acaaagattc taaaaaagtc
    68941 aagctatttt agtttagcct ttgtcaatcg tatcagttca aatagaaaat acaagttttg
    69001 acaagaattt atttatgtaa caaactgtgt ttttgattcc ttcagtctat ctagcgataa
    69061 tgttatctga ttcttgtttt caaccaattg atgttaaaga agctgtcttc atgattctct
    69121 aacattcctt cagttttagc tttatattac tctaagttat cagtaatgac attaagtagt
    69181 ttaacaagaa taatcctaaa agctacttta caaaataacg tattatcata ctaaacaaaa
    69241 gtactgacat actaaaaaca ggctta
//
LOCUS       DAUGFP010000005        67564 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.005, whole genome shotgun sequence.
ACCESSION   DAUGFP010000005 DAUGFP010000000
VERSION     DAUGFP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67564
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.005"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            275..345
                     /locus_tag="VH663_000298"
     tRNA            275..345
                     /locus_tag="VH663_000298"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:307..309,aa:Cys,seq:gca)
     gene            864..1067
                     /locus_tag="VH663_000299"
     CDS             864..1067
                     /locus_tag="VH663_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEQ8173528.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            1263..1724
                     /locus_tag="VH663_000300"
     CDS             1263..1724
                     /locus_tag="VH663_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEQ8173529.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            1724..4168
                     /locus_tag="VH663_000301"
     CDS             1724..4168
                     /locus_tag="VH663_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ8173530.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSYGKLTFHIA"
     gene            4346..4636
                     /gene="groES"
                     /locus_tag="VH663_000302"
     CDS             4346..4636
                     /gene="groES"
                     /locus_tag="VH663_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEQ8173531.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            4672..6303
                     /gene="groL"
                     /locus_tag="VH663_000303"
     CDS             4672..6303
                     /gene="groL"
                     /locus_tag="VH663_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEQ8173532.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            6554..6820
                     /locus_tag="VH663_000304"
     CDS             6554..6820
                     /locus_tag="VH663_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8173533.1"
                     /translation="MNHFGEIFKTFRESKGLRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMSIDEFMYAVHDFHRDDLNELLSKSEGFRNNSR"
     gene            complement(7548..9047)
                     /locus_tag="VH663_000305"
     CDS             complement(7548..9047)
                     /locus_tag="VH663_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ8173534.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDLSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVAKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVEEKPKNKKGLNRS"
     gene            9294..10934
                     /locus_tag="VH663_000306"
     CDS             9294..10934
                     /locus_tag="VH663_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173535.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLISGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDNSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDQKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALVPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(11023..12024)
                     /locus_tag="VH663_000307"
     CDS             complement(11023..12024)
                     /locus_tag="VH663_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ8173536.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKHPLTQEDICVQAKSATFDAVLNAQKARK"
     gene            12600..12980
                     /locus_tag="VH663_000308"
     CDS             12600..12980
                     /locus_tag="VH663_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ8173537.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            13019..15355
                     /locus_tag="VH663_000309"
     CDS             13019..15355
                     /locus_tag="VH663_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ8173538.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKARLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            15404..15556
                     /gene="rpmG"
                     /locus_tag="VH663_000310"
     CDS             15404..15556
                     /gene="rpmG"
                     /locus_tag="VH663_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ8173539.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            15570..15746
                     /gene="secE"
                     /locus_tag="VH663_000311"
     CDS             15570..15746
                     /gene="secE"
                     /locus_tag="VH663_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEQ8173540.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(15982..16755)
                     /locus_tag="VH663_000312"
     CDS             complement(15982..16755)
                     /locus_tag="VH663_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEQ8173541.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            16881..17627
                     /locus_tag="VH663_000313"
     CDS             16881..17627
                     /locus_tag="VH663_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ8173542.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLEVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKVITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSASGLVLDFVS"
     gene            17643..18623
                     /locus_tag="VH663_000314"
     CDS             17643..18623
                     /locus_tag="VH663_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEQ8173543.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRKQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            18911..19231
                     /locus_tag="VH663_000315"
     CDS             18911..19231
                     /locus_tag="VH663_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ8173544.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            19259..19567
                     /locus_tag="VH663_000316"
     CDS             19259..19567
                     /locus_tag="VH663_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ8173545.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDKSEAICKDNDIPIAVIQMADYGMLDGNKVLDLALSLVKE
                     "
     gene            19577..20881
                     /locus_tag="VH663_000317"
     CDS             19577..20881
                     /locus_tag="VH663_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ8173546.1"
                     /translation="MAKMNMQNIIKPIMTFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSDVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEAIGDAISKVWFGGQGLIGAIVIGLTVGAVYTTFIR
                     RHIVIKMPDQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQATEES"
     gene            21092..23509
                     /locus_tag="VH663_000318"
     CDS             21092..23509
                     /locus_tag="VH663_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEQ8173547.1"
                     /translation="MTETKSPYFGHLTDRMAHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKEMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGQLTPEELWHALESDFAGERGEDIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            23522..24190
                     /locus_tag="VH663_000319"
     CDS             23522..24190
                     /locus_tag="VH663_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ8173548.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYKGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAINRENA
                     DSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            24247..25335
                     /locus_tag="VH663_000320"
     CDS             24247..25335
                     /locus_tag="VH663_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEQ8173549.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGDSPILLCDDVVYGIVG
                     ERFESYLIDNSMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMAGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(25417..25926)
                     /locus_tag="VH663_000321"
     CDS             complement(25417..25926)
                     /locus_tag="VH663_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEQ8173550.1"
                     /translation="MSTDHHLREVSYQLLAERGGSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGYSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            26289..27104
                     /gene="sdaB"
                     /locus_tag="VH663_000322"
     CDS             26289..27104
                     /gene="sdaB"
                     /locus_tag="VH663_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEQ8173551.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            complement(27345..28187)
                     /locus_tag="VH663_000323"
     CDS             complement(27345..28187)
                     /locus_tag="VH663_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ8173552.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIEGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            28686..28856
                     /locus_tag="VH663_000324"
     CDS             28686..28856
                     /locus_tag="VH663_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173553.1"
                     /translation="MDNPKKDKGLSLGVVDPITILITESVINKMRTVPYHLKKLSSTT
                     YCLLADLAYQSA"
     gene            29128..30324
                     /gene="speB"
                     /locus_tag="VH663_000325"
     CDS             29128..30324
                     /gene="speB"
                     /locus_tag="VH663_000325"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEQ8173554.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDVNGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSAGSSRVQRALKENFGYNQSVHQINRGDFSKQDWEAQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            30326..30631
                     /locus_tag="VH663_000326"
     CDS             30326..30631
                     /locus_tag="VH663_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEQ8173555.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMVEAYQKQINSLD"
     gene            31111..32040
                     /gene="prsA"
                     /locus_tag="VH663_000327"
     CDS             31111..32040
                     /gene="prsA"
                     /locus_tag="VH663_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ8173556.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTIAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(32311..32547)
                     /locus_tag="VH663_000328"
     CDS             complement(32311..32547)
                     /locus_tag="VH663_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173557.1"
                     /translation="MIHRFFLTPLSTLWRRLRPKRYSKKVLADEPTGYQPPAAHGPCD
                     ETHQLLEDKSLSDDYLIATSAMRHLWYWGYSQIG"
     gene            32971..35997
                     /locus_tag="VH663_000329"
     CDS             32971..35997
                     /locus_tag="VH663_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ8173558.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSTGASSTETSAS
                     TNTSTASAGTSTSETAASGTGSEAAVVSSEGSQSSESGQASTQPQAQTLEQSAATSPS
                     SNSSTSSSEDKAPKAASTKSSSATVASSSNGSNQGAGAEDAPQMMDVERYTVDREKTE
                     LNIKDGKTPKTRNSVDKDTKLIRNRDGKQRDIVDIKREVKDNGDGTLDVTLKVTPKEI
                     DKGADVMALLDVSKKMTDADFKNAKDKIKKLVTTLTSKSASNSDNDEHKHNSRNSVRL
                     MTFYREISNPIDISGKTDAELDKLLDDLRVKAKANYDWGVDLQGAIHKAREIFNKEKE
                     SKKRQHIVLFSQGESTFSYDIQKSEKEKSSNLSRINEKITSSNPLLPWPPIFDHTHQN
                     GDMINDVRALIAFASKLGIKNLSWIETSLSALSVGSNLLGSVFGGGGLGEYLTLKEYD
                     SQKLNEDQFDYTKRVGEGYHYHSFSNRESEDKMPLETEIKKALEAALPKFEENNWFTK
                     VLKYFGLKDKAEQAKLDVIMKVIKSVFYKRQYHYYNHNLSAIAEAKMAQKEGITFYSV
                     DVTALNTARRVKRQTAVLKNTKEEERNKKFDEYLKKMSEGGNAFFNDVDKADKFKDTL
                     TKLTIKDEFDGKVSVDKTSYQSSSAINYSEASNSFWRTTKESLTWTISKEQLKKAFED
                     GKPLTFTYKLKVDNNKFKTALEENNKKKRTKRSTPTENENSVTEKIISNTTTYEINEQ
                     KVEGNKLGDVSLTYSKFKVPVPQIDGHVIEPQAPTLPKLPPVIEHGPNFEYEEETGYQ
                     LPLKHGSNAPDTQVTIEEDTVPQRPDILVGGQSGPVDITEDTQPGMSGSNDATVVEED
                     TAPKRPDVLVGGQSEPIDITEDTQPSVSGSNDATVVEEDTVPQRPDILVGGQSDPIDI
                     TEDTQPGMSGSNDATVIEEDTKPKRFFHFDNEPQAPEKPKEQPSLSLPQAPVYKAAHH
                     LPASGDKREASFTIVALTIIGAAGLLSKKRRDTEEN"
     gene            36177..38138
                     /locus_tag="VH663_000330"
     CDS             36177..38138
                     /locus_tag="VH663_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ8173559.1"
                     /translation="MTKKFIKSAVILGLASITISMTQPVEAISNRLEYQEQYKRVKEE
                     LDALKEKSPKLGERSLYFRLVNRFLGWFGVVWDPLAIKETDHLLDLIGQVNYLVSNTE
                     FQAPEYQKAKNKLDLAVAGYDLYLAREKKRGDAPDYQVTQLTLGQLETIKTKIQEALK
                     TFAQEVKTIQQKTPDLFPHAYVLNAKTSAIDGKAQPFLVNGYYDVFYKQFLFTNKNDG
                     YNYNKEADNLTSYQNLIKELLDLKAEIDRLTKDDPKNDRLETLMWQSKFTLSDFRRTS
                     RLSAYYTQGDYVKTVVLSEEAPNLRTAKEKVKTLKGILHDYYVETNKPEKAKEYRVED
                     ETLPSQPEAPVTPEAPAPSPVPGQKPAEAERSSQATEPANPSKENSSTDASQGSHDSE
                     NPATDSPSQPQAPDQGNHQSQVPNDKPQTDKTDTPNVPAPPQNTPNAPAIPQDTPKVP
                     EAGGQSGPAGNAEEKAPDSGPKASDQSSSKESPSVGEDTTPNQPDVLVGGQSEPIDIT
                     EDTITDAPPTVSGHNASTQPQSVVEDTAPQRPDVLVGGQSEPIDITQDTQPGMSGSND
                     ATVINEDTKPKRVFHFDNKEPQASEKAAEQKLAPHDSHTTPQASDDTAAPHLPASGDK
                     PEKAFALAALTMVAAAGLLSKKRRNTDKN"
     gene            38280..38576
                     /locus_tag="VH663_000331"
     CDS             38280..38576
                     /locus_tag="VH663_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEQ8173560.1"
                     /translation="MTKQDQLIVEKMEQTYETFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADILDLAPIMYKHM"
     gene            38725..39009
                     /locus_tag="VH663_000332"
     CDS             38725..39009
                     /locus_tag="VH663_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEQ8173561.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            39433..40701
                     /locus_tag="VH663_000333"
     CDS             39433..40701
                     /locus_tag="VH663_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ8173562.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATAATEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            40688..41362
                     /locus_tag="VH663_000334"
     CDS             40688..41362
                     /locus_tag="VH663_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173563.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            41375..42592
                     /locus_tag="VH663_000335"
     CDS             41375..42592
                     /locus_tag="VH663_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173564.1"
                     /translation="MEDIIAALSSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            42686..43339
                     /locus_tag="VH663_000336"
     CDS             42686..43339
                     /locus_tag="VH663_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8173565.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            43336..44724
                     /locus_tag="VH663_000337"
     CDS             43336..44724
                     /locus_tag="VH663_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ8173566.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            44826..46454
                     /locus_tag="VH663_000338"
     CDS             44826..46454
                     /locus_tag="VH663_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ8173567.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNTSSTDQPTTAD
                     TDTDDESETAKKDKKSKETASQHDTQKDHKSSHTHPTPPSNDTKQTDQASSEATDKPN
                     KDKNDTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKSADKT
                     PEKGPEKATEKTPEPNRDAPKPIQPPLAAAAPVFAPWRESDKDLSKLKPSSRSSAAYV
                     RHWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVR
                     AIVAIAMAESSLGTQGVAKEKGANMFGYGAFDFNPNNAKKYSDEVAIRHMVEDTIIAN
                     KNQTFERQDLKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMTKLDQWIDDHGSTP
                     EIPEHLKITSGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVD
                     RYMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILIS
                     ESNVMGLGTISYRTFTAEQASLLTYVVGDKLPRP"
     gene            46532..46783
                     /locus_tag="VH663_000339"
     CDS             46532..46783
                     /locus_tag="VH663_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173568.1"
                     /translation="MIKKVTTPSQKIKKRVRNGYLLKLGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
     gene            47459..49051
                     /locus_tag="VH663_000340"
     CDS             47459..49051
                     /locus_tag="VH663_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8173569.1"
                     /translation="MHVSKLFTSQQWRELKLISYLTENSNAIGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHQCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGDERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKDCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYSCSYNNKKTSHRFSQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMFSNHLNDKLE
                     IGCAFEIINQDPTSGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDI
                     TANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFIT
                     WENSFLKVNQRDEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIE
                     KQYDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSP
                     SKSHSDKEVQKPDNSVKQATSS"
     gene            49238..50515
                     /gene="mrp"
                     /locus_tag="VH663_000341"
     CDS             49238..50515
                     /gene="mrp"
                     /locus_tag="VH663_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-related protein Mrp"
                     /protein_id="HEQ8173570.1"
                     /translation="MSKRNPNKHYSLRKLKTGTASVAVALTVLGTGLANTTDVKAETV
                     GRFSDEQVRKAREKAIEDVFDGYTGARSVYQSGNLPNRLTPTKLSKLMQQMYKETLQK
                     KEELDTLSKALTHTIEKKIESENAYKKELGQLKAAAEAEAQKALDALNNKNKQISDLT
                     NENAQLKEAIEGYVQTIQNASREIAAKQQELAAAKSQLEAKNAEIEALKQQDASKTEE
                     IAKLQSEAATLENLLGSAKRELTELQAKLDTATAEKAKLESQVTTLENLLGSAKRELT
                     DLQAKLDAANAEKEKLQSQAAALEKQLEATKKELADLQAKLAATNQEKEKLEAEAKAL
                     KEQLAKQAEELAKLKADKASGAQKPDTKPGNKEVPTRPSQTRTNTNKAPMAQTKRQLP
                     STGEETTNPFFTAAALTVIASAGVLALKRKEEN"
     gene            50740..51843
                     /locus_tag="VH663_000342"
     CDS             50740..51843
                     /locus_tag="VH663_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ8173571.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFANQTTVKADSD
                     NINRSVSVKDNEKELHNKIADLEEERGEHLDKIDELKEELKAKEKSSENVERHYLRKL
                     DQEYKEQQERQKNLEELERQSQREVEKRYQEQLQKQQQLETEKQISEASRKSLSRDLE
                     ASRAAKKDLEAEHQKLKEEKQISDASRQGLSRDLEASREAKKKVEADLAALTAEHQKL
                     KEEKQISDASRQGLSRDLEASREAKKKVEADLAEANSKLQALEKLNKELEEGKKLSEK
                     EKAELQAKLEAEAKALKEQLAKQAEELAKLKGNQTPNAKVAPQANRSRSAMTQQKRTL
                     PSTGETANPFFTAAAATVMVSAGMLALKRKEEN"
     gene            52053..53159
                     /gene="enn"
                     /locus_tag="VH663_000343"
     CDS             52053..53159
                     /gene="enn"
                     /locus_tag="VH663_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-related protein Enn"
                     /protein_id="HEQ8173572.1"
                     /translation="MARQQTKKNYSLRKLKTGTASVAVALTVLGAGFANQTEVRAEGV
                     KATTNLPEEAKYAALKDENTGLRGDQTKLVKKLEEEQEKSKNLEKQKQELENQALNFQ
                     DVIETQEKEKEDLKTTLAKATKENEISEASRKGLSRDLEASRAAKKELEAKHQKLEAE
                     NKKLTEANQVSEASRKGLSNDLEASRAAKKELEAKYQKLETDHQALEAKHQKLETDYQ
                     VSETSRKGLSRDLEASREANKKVTSELTQAKAQLAALEESKKLSEKEKAELQAKLDAQ
                     GKALKEQLAKQTEELAKLRAEKAAGSKTPATKPANKERSGRAAQTATRPSQNKGMRSQ
                     LPSTGEAANPFFTAAAATVMVSAGMLALKRKEEN"
     gene            53494..57042
                     /gene="scpA/B"
                     /locus_tag="VH663_000344"
     CDS             53494..57042
                     /gene="scpA/B"
                     /locus_tag="VH663_000344"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEQ8173573.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VETPQPTAVSEEAPSSSKETNPPQIPDDAEETVADKANDPAPQAPAKTADTPVTSKAT
                     IRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKA
                     KKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTADKQDKEMPVLSTNRFEPNKAYDYAYANRGMKEDDFKDVKGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     PKKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDTFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMK
                     NGYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSNKPEQDGSDQAPDKKPETKPEQDGSGQAPDKKPEAKPEQDGSGQTPDKKPEAKPE
                     QDGSGQTPDKKPETKPEKDSSGQTPGKTPQKGQPSRTLEKRSSKRALATKASTRDQLP
                     TTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRTED"
     gene            57139..58374
                     /locus_tag="VH663_000345"
     CDS             57139..58374
                     /locus_tag="VH663_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ8173574.1"
                     /translation="MLRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDSMQQN
                     SSTQRNTANTSKKWYHIDDRGFIPLGISLEAAKKDFKEEVEKSRLSEEQKQTYNQKID
                     AETDKDELLSTYHRDYLTAVKNLPTSTEPVEAPVQETQASVSDSMVTGDSTSVTTDSP
                     EETLSTETPSPSPESEKSSVAASSEETSSPETPSTPEAPVAPALSEAPSPSPESEEPS
                     VAASSEETPSPSTPAAPETPAAPETPAAPETPEEPSVAAPSEETPSPSTPAAPEIPAE
                     SEAPAQPAESEESSVAATTSPSPSTPAESETQTPSAVTKDSDKPSSAAEKPAASSLVS
                     EQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVMAHESGKYLPSTGEKAQPLFIATMT
                     LMSLFGSLLVTKRQKETKK"
     gene            58626..59546
                     /gene="lmb"
                     /locus_tag="VH663_000346"
     CDS             58626..59546
                     /gene="lmb"
                     /locus_tag="VH663_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEQ8173575.1"
                     /translation="MKKGFFLMAMAVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            59559..62030
                     /locus_tag="VH663_000347"
     CDS             59559..62030
                     /locus_tag="VH663_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEQ8173576.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGPATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYRFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YMMSKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQAD
                     KSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQP
                     EGVQFYNKNGELVTYDIKTLQQINP"
     gene            62199..62399
                     /locus_tag="VH663_000348"
     CDS             62199..62399
                     /locus_tag="VH663_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ8173577.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(62479..63105)
                     /locus_tag="VH663_000349"
     CDS             complement(62479..63105)
                     /locus_tag="VH663_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173578.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIIISHDFDMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(63089..63892)
                     /locus_tag="VH663_000350"
     CDS             complement(63089..63892)
                     /locus_tag="VH663_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173579.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIKQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGGGEQLQTEFARRLWRTLPQQDFLKGVTHDLRG"
     gene            complement(63904..64725)
                     /locus_tag="VH663_000351"
     CDS             complement(63904..64725)
                     /locus_tag="VH663_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173580.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVFLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(64722..65699)
                     /locus_tag="VH663_000352"
     CDS             complement(64722..65699)
                     /locus_tag="VH663_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173581.1"
                     /translation="MKRTTIIIIWKIIRCVTLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVIRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNFIADILNSIINPQLRRKV"
     gene            complement(65812..67440)
                     /locus_tag="VH663_000353"
     CDS             complement(65812..67440)
                     /locus_tag="VH663_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ8173582.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELADKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGIHSHGHDWSLLTNIAEWTWDESTK"
BASE COUNT        22718 a        13273 c        13596 g        17977 t
ORIGIN      
        1 acattaatta aaatgattct aaataagctg aagacgccgt taaatcaaag gttaatttaa
       61 ttataatcta cagaaaatgt aattattatt aaagcgttta caaaaaaact tttttgattc
      121 cgttaaggca acttaatatt atggttacct aatcgctaat attgtaacct attttggcat
      181 gagatcaggg aatggctgtt atattaattg gatatgtctt gtgcaaatag tgcttgacgg
      241 atttgtggaa ttagggtaaa ataatctttg ttgtggcggt atagccaagt ggtaaggcac
      301 ggctctgcaa aagcttgatc gtcggttcaa atccgtctac cgccttttat atacaaaaca
      361 agccttgatt aatttcgtga aaacgttgtt aaatcaaggt ttttattttt tgcgacagga
      421 taaaaaaaga gaaaaaaaga aataaagtcg gtcaaaagtc ggtcaaattt cccgaagatg
      481 tcttttatag cctgattatt aggtatgaaa aatactatgg ccaaaattgg taaacgattg
      541 caaataaagg tcagagttct taccccctta aacgatatgc gttaaaagtt agttgaactg
      601 ccgtgcacct aacgatagac acggtgatgt gagagcgagt agagttgaat ctctacttgt
      661 ttatcttacc atagtcagct ttataaagta accacccatt acaatgcaac tatattctta
      721 atgtttttaa gcatgttatt gacaagtgtt ataaaatgtg ttactcttag tgagtaaatt
      781 agtttttgag ttagtatttg tgtttcaaca tctttatttc tctctatttt tacaaatgat
      841 ttttagaaag gaagtggatg tatatggcac aaggtacagt aaaatggttt aacgctgaaa
      901 aaggttttgg ttttatttca actgaaaacg gtcaagatgt ctttgcacat ttttcagcca
      961 tccaaactaa tggttttaaa acgttagaag aaggacaaaa agtggcattt gacgtcgaag
     1021 aaggtcaacg tggacctcaa gcagtcaata tcactaaatt ggcctaatct ggttaaagta
     1081 actaaagaga agcagatgtt ggaaacaaca actgcttttt tgatttctac cagttacctt
     1141 taatgattaa gagttgcgtt taaggtcttg tatttgttat aataatagtc agttttagtc
     1201 aaaatgtatt gacgtggtgc tactaagttt ttagagtagc ccgcaagcaa aggagtgaca
     1261 gcatgccaac caaaaatact tcagacagca ttgaagagta tattaaagaa ttattagcta
     1321 agtcaggaat agcagaaatt aagcggtcca tgctagcaga ttcgtttcaa gttgtcccaa
     1381 gtcagatcaa ctatgtcatt aaaacccgtt ttacggagag tcgaggctat gaagtagaga
     1441 gtaaacgagg tggtggaggc tatattcgta ttgccaaggt tcatttttca gacaaacacc
     1501 atctgattgg caatttaatg gcaactattg aagactgtat cagtgagcaa gttttcacgg
     1561 actcgataca attgcttttt gacgaacact tattgacaga gcgtgaggga aatatcattt
     1621 tggctgtggc gtctgatgat gttttaggaa ctgatggttc aactattcgt gcacgaatgc
     1681 tctatcgctt attgcaacga attgacagaa agggaagcaa ttaatgatta tgtattcaac
     1741 gaagatgcaa gacattttta gacaggcgca gttccaagct gctcgctttg atagccattg
     1801 cctggaaact tggcatgttt tgttagctat ggtagctgta gataattctt tagcaaatat
     1861 gattttaagt gaatatgatg cccaagtcgc catagaagaa tatgaagctg cagctatttt
     1921 agccatgggc aaaaccccta aggaacagtt gtctcgtgta gacttcagac ctcaatctaa
     1981 aaccttgact aacttgttag cttttgcgca ggctattagc caaatcacta gggatcaaga
     2041 agtcggctct gagcatgtct tatttgctat tttattgaat ccagatatca tggcgagtcg
     2101 tttgttagaa atagcaggct atcagataaa agataacggc aatgggcagc cgcgattagc
     2161 tgacttgcga aaagcaatag aacgtcatgc aggttacagt aaggaaatga ttaaggctat
     2221 tcacgaacta cgtaagccta aaaaaacgaa aacacaaggg accttttcag atatgatgaa
     2281 gccaccaagt acagctggtg aattgagtga tttcacaaga gatttgactg aaatggcaag
     2341 acaaggtttg ttagaatcgg tgattggacg tgaccaagaa gtatctcgta tgattcaggt
     2401 actaagtcgt aaaacgaaaa acaatcctgt cttggtaggt gatgcaggtg ttggtaaaac
     2461 tgcgcttgct tatggccttg ctcaacggat tgcaaatggc gctattcctt atgaacttaa
     2521 ggagatgcgt gtcctagaat tagacatgat gagtgtggta gcaggaaccc gttttcgtgg
     2581 ggattttgaa gagcgcatga atcaaatcat tgatgatatt gaagctgatg gtcagattat
     2641 tctttttgtt gatgaactac atactattat gggttctggc agtggtattg acagtacact
     2701 tgatgcggct aacattttaa aaccagcatt atcgcgcggt actcttcata tggttggagc
     2761 aacaactcaa gaagaatatc aaaaacatat tgaaaaagat gcagctcttt cgcgtcgttt
     2821 tgctaaaata ttaattgaag aacctaatac agaagatgct tatcagattt tgatgggcct
     2881 aaaattatct tatgagacct accataatgt ctcgatatca aatgaggcag ttaaaacagc
     2941 tgtaaaaatg gcacaccgtt atttaaccag taaaaatctc cctgattcag ctatcgattt
     3001 attagatgaa gctagtgctg ctgtgcaaaa catggtgaaa aaatcagctc ctgagacttt
     3061 aacaccaata gaccaagctc ttatcaatgg tgatatgaaa aaagtatctc gcctcttagc
     3121 taaagaagca aaaggtcaga tgagaaaacc aacaccagtg acagaagatg atattttggc
     3181 aaccttgagt aagttatcgg gaattccact tgaaaaactg acgcaagctg atagtaaaaa
     3241 atacctcaat ttagaaaaag aactgcataa gcgtgtgatt ggtcaggatg ctgctgttac
     3301 ggctatttca agagccattc gtcgcaatca gtcaggtatt cgaacaggaa aacgtcctat
     3361 tggatcattt atgtttcttg gcccaacagg agtaggtaag acagaactag caaaggccct
     3421 tgcagaagtt ctctttgatg atgaagcagc gcttattcgt tttgatatgt ctgagtacat
     3481 ggaaaaattc gcagcgtcta ggcttaatgg agcacctcct ggttatgtcg gctatgatga
     3541 aggaggtgaa ctgacacaga aagttagaaa taaaccttat tcagtcttgc tttttgatga
     3601 agtggaaaaa gcacatcctg atatttttaa cgttctcctt caagtattag atgatggtat
     3661 attgactgat agtcgtgggc gtaaggtcga tttttcaaat actattatta tcatgaccag
     3721 caatcttggc gcaacagccc tgcgtgatga taaaacggtc ggttttgggg tcaaaggcat
     3781 tcaccaagac catcaagcta tggagaaacg tattttagaa gaattaagaa aaacttaccg
     3841 cccagaattt attaatcgta ttgatgaaaa agtggtcttt catagtctga cccaagataa
     3901 catgcgcgat gtggttaaaa tcatggtaca gcctctgatt actacattgg cagaaaaagg
     3961 tattaccctt aaaattcagc ctttggcctt gaaacatttg tccgaggtcg gctatgatga
     4021 gcatatgggg gcaagaccat tacgtcgaac gctgcaaact gagatagaag ataagctatc
     4081 agagcttatc ctttctcgag aattgacaag tgggcatacg ctaaaaattg gattatcata
     4141 tggcaaatta acgtttcaca tagcttaatt tccatataaa aggtggatca tccttgatgt
     4201 caccaaaaaa agaggttatt cttacaaaaa taacttcttt tttcttgact atttttgacc
     4261 aagtgataaa atagaatata aagttagcac tcgatgaaca tgagtgctaa ctttactaaa
     4321 gattctttta ttggaggaaa atttcatgtt aaaaccatta ggtgaccgtg tggtcgtaag
     4381 atttgatgat gaaaaagagc agacagtcgg tggttttgtc cttgcaggca ctcataaaga
     4441 gtcaacacga aaagcgacag tcctcgctgt cagtgaaacg ggtgttcgca ctattacagg
     4501 tgatagcgtg ctaccttcag tttcagtggg tcaagaggtt ttagttgaaa atgggcatga
     4561 tttagaagta accgtagatg atgagaaagt ttctatcatc cgtgaatctg acatcattgc
     4621 tattgttaca aaataaaacg acaaatctct aaatagaaag aaggattgaa tatggcaaaa
     4681 gatattaaat tttcagcaga tgcgcgtgct gccatggtgc gcggagttga tatgttagca
     4741 gataccgtca aagtaacgct tggtcctaaa gggcgtaatg ttgttcttga aaaagctttt
     4801 ggttctccct taattactaa tgacggggta accattgcta aagagatcga attagaagat
     4861 cattttgaaa acatgggagc aaaattggtg tctgaagtgg cttctaaaac caatgatatt
     4921 gctggtgatg ggacgactac tgcaacagtt ttgacacaag ccattgttca tgaaggacta
     4981 aaaaatgtga cagcaggtgc taatccaatt ggtatccgtc gaggcattga aacagcaaca
     5041 gcaacagctg ttgaagcctt gaaagccatt gctcaacctg tatctggcaa ggaagctatt
     5101 gctcaggtcg ctgcagtatc atcacgctct gaaaaagttg gagagtatat ctcagaagct
     5161 atggagcgtg tgggcaacga tggtgtgatt accatcgaag aatctcgagg tatggaaaca
     5221 gaacttgaag tggtcgaagg catgcaattt gaccgtggtt acctgtctca atacatggtc
     5281 acagacaatg aaaaaatggt tgcagacctt gaaaacccat ttatcttaat cacggataaa
     5341 aaagtgtcaa acatccaaga cattttgcca ctacttgagg aagttcttaa aaccaaccgt
     5401 ccattactca ttattgcaga tgatgtggat ggtgaagccc ttccaaccct tgtcttgaac
     5461 aagattcgtg gtactttcaa tgtggttgct gtcaaagcgc caggatttgg tgatcgtcgt
     5521 aaagctatgc ttgaagacat tgctatcttg acaggtggta cagtgattac agaggatcta
     5581 ggacttgaat taaaagatgc tacaatgaca gcccttggac aggctgctaa gattacagtt
     5641 gataaagata gcacagtaat tgttgaaggt tcaggaagtt cagaagctat tgctaaccgt
     5701 attgcactga ttaaatcgca attagaaaca acaacttctg actttgaccg tgaaaaacta
     5761 caagaacgtt tggcgaaatt agctggtggt gtagctgtta tcaaagtagg agctccaaca
     5821 gagacagctt taaaagaaat gaaacttcgc attgaggatg ctctaaatgc tacacgtgca
     5881 gccgttgaag aaggtatcgt tgctggtggt ggaacagcac ttattacggt tattgaaaaa
     5941 gtagcagctc ttgaacttga gggcgatgat gctactggac gtaacattgt gcttcgtgct
     6001 ctagaagagc ctgtacgtca aattgcttta aatgctgggt acgaaggctc cgtagttatt
     6061 gacaagttga aaaacagccc tgcaggaaca ggatttaatg ctgcaacagg tgagtgggtt
     6121 gatatgatta aaacaggaat cattgaccct gtcaaagtaa cacgatcagc gcttcaaaat
     6181 gcagcttctg tagctagtct tattttgaca acagaagcag ttgttgctaa taagcctgaa
     6241 ccagctgcgc cagcgccagc aatgccagca ggtatggatc caggaatgat gggcggcttc
     6301 taaggtctct atagcattaa ggattatgaa aaagaagttg agactaaagg tctgcttctt
     6361 actatgaaaa aagaacaaag attggatttc gactcatgat atcccatctt tgttttttta
     6421 gttgtgatat aaaatgccaa tctttgtggt tacagacttt taatgtattg gaaaatatat
     6481 tgtttttctt tgtcatgtgc tatcatggag ccaatgaaaa gttattccca tatatgcgtg
     6541 gtggaggtct gtgatgaatc attttggaga gatttttaaa acctttagag aatcaaaggg
     6601 attacgatta aaagatgtcg caaaggctgg tatatcaacc tctcagctgt cgcgtttcga
     6661 aaaaggggag acggatttaa ccatatcgac atttatgctg attttggatg aaagtaacat
     6721 gtccattgat gagtttatgt atgctgtcca tgattttcat cgtgacgact taaatgaact
     6781 cttatcaaaa agtgagggat ttcgtaacaa ttcaagatag tgatggcttg aagcagttac
     6841 ttcatgctca actagcatca actgataaga aggaaatgtt cacggatatc ttttggcata
     6901 tctagcatgt ctaagtctgc tatggcatgc cattttggga gtccctcacg cgactctgtc
     6961 agtagctctc cttcaaagga ttcacataga aagtcgtaat agacatagca ctcttttttt
     7021 gtcaggcttg ataaagctag aaataccctt taattgaagg tttaaagctg tcagacctat
     7081 ttcttctttt agttctcata aagccgcttc aaagaaagat tcacagggct cgactttacc
     7141 ttctggctgg acccatcctg gtaagttatc gtgttgtcta tggagtcata aaatcatatc
     7201 gttatgtttg acacacatat tgccccagtc tctaaccgtt gctatcacca gattcaccac
     7261 cttttagtat agtataagaa aaaaggggtg aaagaggttt cttgttgttt ttttggctta
     7321 agatttcttt aagtcttatc tagtatacta atgtcattcc ttaataggat aagtatttgg
     7381 ttgtagaagt atcgtcttaa actaacgcta tctcaaccga ttcccaactt attcacccct
     7441 cctataccga taaggtctca caaagtgtga gaccttattt tttgacttca gctctagtta
     7501 tggagaaact tgttgataca agcttttttg ggttatgggt tatggtatta tgatctattc
     7561 aagcctttct tgtttttggg tttttcttca accaacttgg cttcaatagc ttttaagcgt
     7621 ttgaaggttt tttcagcgcg atctagtgaa atcttatcag ctttttcttg agcagctttg
     7681 gggtcactga cgactaagtc actgatttct ttggtgcttt tgtcttgttc ggctatccag
     7741 gttttttcca agcctttcat ctcatcaatc actttagttc caaatggttt tggatgagca
     7801 gccacgagat cattaatgtg tgacaccgtc caataccatg acttatcgtt atattgggtg
     7861 cttttttctt gataggcttc ataggttcta ctgatattgc caaggtatgg caagtaaggg
     7921 gcattacgag gactgccaat agatagccac atgacaccac cacccaattc agcagggatg
     7981 tcttttttca gttggaaaat atgagcttcc atgacattag gatttgagat gggataagca
     8041 taacctttaa cagctttttt ggattttggt ttacccttac cgtctaatgc catctgatca
     8101 agtggtttaa gatccagccc ctcaaaacga ttacgttgca gtttcatcgc atcttctaga
     8161 ctgaaggtct tatcagtgct ttggagcaac tcataattgc tgtctttata agttactttt
     8221 gaatcagggt caagtgattt aatccctgag aaagagcgag agcggttggc atcgtttagt
     8281 ggtggattgt aagatttggc aatatgaaat ttaccatcaa cttcggtata agatttggct
     8341 tttttagcga ctttttccac atcttctgag gcaatcgtat tttccttatc gttgaaatcg
     8401 acatgtccga gatagaaggt atttggaaaa acagcgtatt tatcatctgg gaatttgatg
     8461 gcaacatatt ggtgaccaga gagaatttcc atgtaccaga tgccatcttt gtcagccaag
     8521 gtaacaatat ttccttcagc agctcctttt tcagtgacta ttttagcaat caatgcgaca
     8581 ccttcacggg ctgttttgac gcttggtaaa atgacactgg ccatagatga ttccgctaag
     8641 ccatttttga cgtaaggatc aattttttgg atggcatcat tagctgaagc tgatactgtt
     8701 gcagacatag agacgccaaa ctcattaaaa ccagcttcgt cataaacgcc tttgttaggt
     8761 gttacatcag gaatcgcact atagcgataa gaatgctcag gcaacgggta ctcaaaacca
     8821 ttagagaggt ctttccattt ttcgccagca ggattgtcct ttgctagacg tacaatgaaa
     8881 tttttattat ggtgtggctc aagatcttct gttcgaccat aaagaagaga accgtctttg
     8941 gttaaatctt tgccaataat aaaaccagta caagcataac taacactttg aagcgaaaag
     9001 gctgtcagaa tacttaaaac tccaagtgat atttttttgt ttatcatgcg aatcctccta
     9061 gtaaacgata acgctttcac agacattata aaatgctctt tttaaaaatt caagtctttt
     9121 atgaagtgtt ttgaaattgt tagctatcta aatattatga gaattctctc ttgaattttg
     9181 gcttaacggc ccctaaaaac gtataggcag tgaactatct aggtttcttc gaaaatccat
     9241 tagccattta tgatataata gtaaagaaaa acatagaaag ttggggtctg ttgatgacca
     9301 aaccatttca tcataagaaa ctaaaacaaa taactataat agctgcaact agcctttttt
     9361 tattcctgat cagtggtgcc ttttactact ctaaaaatca ttgtattaat gcctatctta
     9421 aagctcgatc agcccaatca ggaccagttt ttgagaatat caaagcatat ctagtctggg
     9481 atgatactaa tgagcagatc acaaatgacg aggcgatgta tactaagttt agaaggtata
     9541 gtcaaaaaga attgaggcaa aaaaagcagg atttaaaagc cgctagtcaa gatagtgccg
     9601 ttcaagtaaa gtctgttggt cgtcgttttt ggatttttcc tgattatcgg attgccataa
     9661 aacccatgga tttaacgata aaaacgaatg tgcctcaagc agacgttctt ttaaatcaaa
     9721 aaaaagttgc tgtttctgat tcagaacagt tctcagtcaa gcttgatcgg ctaccaacgg
     9781 cagaatatac cgcaagtatc agaggcaaac acaacgggcg aaacattaaa gtcaacaaat
     9841 catatgatgg tgataatccc gtgctagatt tgagtgtgtc ttttagaact tttttggtaa
     9901 caagcaatgc taagcaagga gatctttact tcgatgataa ccatattggc acattaaaag
     9961 atggtcaatt acaagtagaa gattaccctg ttacagaaaa cgcacaagct tatatgaaaa
    10021 caacattccc agatggtgag ttaagatcac aaaaatatgc tctagctgat gttgaagaag
    10081 gagcaaccct ggagatttta gttacagatc ttttagaaga ggacaaggca ggggagctat
    10141 tagtatcagc ttttgatcag ctgatgcact acctcagtac aggtcaagac tcatctaact
    10201 tacgtagcgt ctttgaggca gggtctagca acgcatttta tagaggattg aaagagtcca
    10261 taaaagcgaa atttcagaca gatacaagaa aagccagccg tctcaatatt ccatctatcc
    10321 ttttgacaac aatgactcaa gtgggtaaaa caacttacgt gcttgatttc acagctacct
    10381 acgaattttt gtatgacaac tcaacagatc ctgagcagca tacctctgga catattaatc
    10441 aagacttgac tggaaaagtg acggtaaaaa aagttggaca gcattacctt atcagccagt
    10501 ccggctccaa aaatattact gttgttaaag aggacaatca actcaaagcg ccatctgtct
    10561 ttcctgagtc tattttggga acgtggacag gccaagccaa tggtttgagc atccatatgt
    10621 ctctagcatc agatggaaca attacgacta aagttgaaga tcaaaaaggc aaccgttcta
    10681 aagaaactcg gacagctaaa attagtaaag ttgaagacaa aggcaatggt ttttatcttt
    10741 atacgccaga tcctggaagt gacataagcg ccttagttcc agaaggagga ttggggggtg
    10801 caaatgtcaa atatgcttat ggtttcaaaa tatctggtaa aacagcctct ccagtggtgt
    10861 ggcaggcagc attaacacat gaatttgatt ataccaagcc actttcggga gtaactttgc
    10921 aaaagcaacc ataaaggatg caaagccaca aagaacagta gaaacaatgt tatcactcaa
    10981 atacagtgct atgaaaacag ctaaactcaa aaggctagga gcttatttcc tagccttttg
    11041 agcgtttaag actgcgtcaa atgtagctga tttagcttgc acgcaaatgt cttcttgggt
    11101 tagagggtgt ttcaaacgta attgataagc atggagcatc agtcgagcac aatctttttt
    11161 tccattagaa tacaaaggat caccaaataa aacatgacct tgatgggcta aatggactcg
    11221 aatttgatga gtccgtccag tttgtaattg acaattgagt aagcttgccg ttttgtggaa
    11281 gtttttgact agagttacct cagtaatagc tttcttgcca tttatcggat cgacaacacg
    11341 ttttctgcgg tcatgtcgat gacgaccaat tggatgatgg taagttaccc taggactgtc
    11401 taaggatccg tgaactagtg ccaaatattc tcgatggatg tctcgttttt ctaaaagacg
    11461 attaaggatt ggtaagataa aaggtgtttt agcaaataaa atggccccac ttgtttcctt
    11521 atccagacga tgaaccacat agcaggtctg tcccgtgtaa gcagacacgt ggttgagcag
    11581 cgccaactct gttggatcat tgccgtgtgt tttcatccct tcaggtttgt taacaataat
    11641 gatgtgttca tcttcataaa ggcaggttac tttttcggcc tgccccatca cgataatctt
    11701 ttcaggataa tcttcgtgat cgaaaaaaag tttgacctga tcaccgtatt taacacaact
    11761 ttgccagtta acaggatgac cattaataag aacatgtttt ttagttctca gaaaatgcct
    11821 aattttacgg ggaattaaca attgttcctc taataatgct ttgaccgtca attcttcaaa
    11881 gggatttttc agtgtgacag taaacccctt tgatggttta tttgttgtct tcattgagcc
    11941 agttatggta tttttcaaca aggttgatag ccatttgggt ggcaagagca gcagagaaag
    12001 cttcagtacc ttcacgctcg tcattagcta cctccaagcg agtctcatcg taaatggctt
    12061 ttagactttc tgcggataag gtttctttaa aagcttggaa tttattcatt ggttaaaccc
    12121 gtccttctat gtttaatgtg agctcctgat taatgcaagg aattcaatgc tattatagca
    12181 tgtttctaat caaaacgtgc cactcaaaaa ggtaaaaacg ttattttgct aatccatata
    12241 ataagaaagt gtcttaaata agacctcttg atagagtttg taacagaaat gatatgaaac
    12301 taggtgctaa cgcagaaata gaaaacaact aacgtgttgc gaagttggaa cagtcataaa
    12361 aaatagcaag ataaaactta tcaacacaca gttacctaag acgatgagtc aaacaggtat
    12421 gttctttcaa aacaacccca tgaggttgaa cgacgtatcg gaaaaatacg gattagtttg
    12481 gccttatgat aggtccttct ctatcgtaaa agaagtcaac caaaggtttt taatataaaa
    12541 tattaagaaa aattgttata attatgataa aaacataatc gtaaaataga ggaaaaacaa
    12601 tgaaaacaat tagacgttat gatgttaacg aagatagagg gcatacaggc cttgtagaag
    12661 caggagattt ctattattta aactattgtg tcggcaatgt cggacaagac attgaaagtc
    12721 agattaatgg agcctttgac gagatggagc gccgattagc attggtgggg ctcacattag
    12781 atgcagttgt tcaaatggat tgtctatttc gagacgtttg gaacatacct gtgatggaaa
    12841 aaatgattaa agagcgcttt aatggcagat atcctgctcg aaaatcgatt caaacggaat
    12901 ttgcacatca cggtggacca caaggactac tctttcaagt ggatggtgtg gcttattcaa
    12961 aacatatttc gatgacctaa tcaaggttac tttaagatca tagtggtata ataacgttat
    13021 gagatttcta gaacttttac aaaagaaatt ttttcctaaa gcatatcagg aaaaacaatt
    13081 cttaatgcat caaaaagcgc gtttaacgcc acaacacaat caaaagcagt attcgccaaa
    13141 tgccaatcat ttggactcat cagctaccaa aaactcagaa caagaccctg caatagctct
    13201 gcaacgcagt agagcctatg aaggaagccc taaaagtcgg cccgcttggt tgcaaaagtt
    13261 ggaagctgtt ttgccgtctc ctcaacatcc aattcggcgt ttttggcgcc gctatcacat
    13321 cggaaaactg ctaatgattc tgattggaac tcttgtctta ctcttaggat catacttgtt
    13381 ttacttatca aaaacagcta aagtatctga tttacaagat gccttgaagg ctacaactgt
    13441 tatttatgat cacaaaggag agtatgcagg cagtttatct ggtcaaaaag ggagttatgt
    13501 tgagctcaac gctatttcag atgatcttga gaatgctgtt attgccactg aggataggac
    13561 tttttacagt aatagcggta ttaatcttaa acgcttctta ttggcggtag ttacggcggg
    13621 ccgctttgga ggtggctcaa cgattacaca gcaactggct aaaaatgctt atctctcaca
    13681 agatcagaca attaaacgaa aggcccgaga gttttttttg gcgttagagt tgaccaaaaa
    13741 atacagtaaa aaagatattc ttactatgta ccttaacaac tcctactttg gaaatggagt
    13801 ttggggagtt gaagatgcca gtcaaaaata ttttggaacc acagctgcta acttaacact
    13861 ggatgaagct gccacattag caggtatgct caaaggacct gaaatatata acccttacca
    13921 ttctctaaaa aatgctactc accgtagaga tactgtttta ggagcgatgg ttgatgccaa
    13981 aaagattacc caaacaaaag ctcagcaagc tagagcagta gggctaaaaa atcgcttagc
    14041 tgatacttat gttggtaaga cagatgacta caaataccca tcctactttg atgctgttat
    14101 tagtgaagca atagcaactt atggtctttc agaaaaagac attgttaata atggatacaa
    14161 agtttacacc gagctagatc aaaattacca aactggcatg cagacgactt ttaacaacga
    14221 tgaactgttt cctgtttcag cttatgacgg tagctctgct caagcagcta gtgttgcttt
    14281 agatcctaaa acaggaggtg ttagaggtct gattggtcgt gtgaatagta gtgaaaatcc
    14341 gactttcaga agttttaact atgcgactca agcaaaacgt agtcccgcat caacaatcaa
    14401 accactcgtg gtttacgcgc cagccgttgc ttcaggatgg tcaattgaaa aagaactacc
    14461 aaataccgtt caagattttg atggctatca gccacataat tatggaaatt atgaatcaga
    14521 agatgttcct atgtatcaag cattagcaaa ctcttataat attccagcag tttctacatt
    14581 gaacgatatc ggaatcgata aagcctttac ctatggtaaa acatttgggt tagatatgag
    14641 ctctgccaaa aaagagttgg gggtagcttt aggtggcagc gtgacaacca atccattgga
    14701 gatggctcag gcatatgctg cctttgccaa taatggagta atccatcctg cgcacttgat
    14761 taaccggatt gaaaatgcca ggggtgaagt gctcaaaacc tttactgata aggctaaacg
    14821 tgttgtcagc cagtctgttg cagataagat gacagccatg atgctaggta ccttttcaaa
    14881 tggaacagca gtcaatgcta acgtatatgg ctatacacta gctggtaaaa cagggacgac
    14941 agaaaccaac ttcaatcccg acttagcagg cgatcagtgg gttattggtt atacgccaga
    15001 tgttgttatt agtcaatggg taggatttaa tcagaccgat gaaaatcatt atctaacgga
    15061 ttcaagtgca ggcacggctt cagctatttt tagcactcag gcatcttaca ttttgcctta
    15121 taccaagggc agccaatttc atgtagataa tgcctacgct caaaatggta tttcagctgt
    15181 ttatggagtc aatgaaacag gtaatcaatc aggagttgac actcaatcta ttattgatgg
    15241 tttaagaaaa tcagcacaag aagcttcgca atcactatca aaagcagtcg atcagtcagg
    15301 gttacgtgat aaagcccaat ctatttggaa agagattgtt gactatttta gatagcttgc
    15361 agtattttag agatcgtgtt aaaataatag ttacggaggc gttatggcac agaaaaaagc
    15421 gagtctagcg tgtgtcgaat gtggcagtcg taactactct atcggagtga gtagcacgcc
    15481 taaaccaaca cgtctagaag taaataaatt ttgcaaatac tgcaaaacat ataccttaca
    15541 caaagaaaca cgttaaaaag gagagcctga tgggattcat tagcgggact tttaaagtct
    15601 taaaagacac aacatggcca aatcgtaaac aacgttggaa agattttatc tcagttcttg
    15661 agtacacagc tttctttacg gtgattatct acatctttga ccagctctta gcaaagagtg
    15721 tattagcact gattaattta ttttaggtca agattggtag aaatgataag cttgcttgga
    15781 aaagcctagg ctgggagtat tcccagcctt ttttgtagta tcaataatct aattagtaag
    15841 ctttttaaag cccatactgg tttgagctat tcaaaagcat aaaggtaaag gcaatgtagc
    15901 aacgatctcc aaaccaagtt cactaaatta gcttgtaaca gagtcaacgc ataaacagcc
    15961 tggcctcact tgcttagcac attaaaaata gcaatgaata ttatggttga ggaaaacagc
    16021 ctgatagtct gctaaatctt cgggatgata agccttgacc tcagccatct catactccct
    16081 acctagaagt ttgtacttgt tttctccaaa ttgatggaaa ggtaacagtt gcacttggtc
    16141 aatctcaagc tgattaaaga gctctgagaa ggctttagca tcgtctagtg aatcattaaa
    16201 ttggggaata acagggatgc gcaagacgat ttcttttcca gcttgaaagg cataatgaat
    16261 atttttgata atcaagtcat tacgaacccc agtcactttt tggtgtctga gctgattata
    16321 atgtttcaaa tcggtataga taaagtctac gtagtcaata agggtgacaa attgctcgtg
    16381 tttggcaaag gcggtggttt caattgcggt gtgaagacct gctgctttgg ctgcttttaa
    16441 cagggctaag gcaaagtcaa attgggcaaa gatttccccc cctgacaggg tcatgccccc
    16501 acctgactct tcgtagaaat caaggtcttt aaggacttct tcgatcacct cgtccacggt
    16561 tttttcctct ccaactattt tagtattaag cccatcactt gttaacatct gctcaggtgc
    16621 tttttgttgg gattcgggat tggcacacca aggacatctc aagggacaac cttttaaaaa
    16681 aacggttgtc cgaataccag ggccgtcatg aatactaaaa tgttggatgt tgaaaacaat
    16741 accgcgatca gtcataagtg tgaattcctt tcgttgtaaa accatcatac cagataaacg
    16801 taaataaatc aattacgaat gaaagtaaat atcccttttc ttttaagaaa ataactggta
    16861 caatgaaagg gaggtgaatg atgaatcgat tagaacgtat tattcaactg gtttcccaga
    16921 agaaaaaaat agatgttaac agtttatcag agcaattaga agtttcgaaa gtaaccattc
    16981 gaaaagacct tgataagctt gaaagtaagg gcttgttgcg acgggaacat ggctatgcgg
    17041 tgctaaacag tggcgatgac ctcaatgtac gcttgtccta caattacaac ataaagcgac
    17101 ggattgcaga aaaagcagct gaattggtgc aagacaacga taccattatg attgaatctg
    17161 gctcaacctg tgcccttttg gcagaagtgc tttgccagac caaacgaaat attaaagtca
    17221 ttaccaattc gtgcttcatt gccaactaca ttcgtcaata tagtagctgt caaatcattt
    17281 tactgggcgg ttattaccag cccaattcag aggtgactgt tggtccttta ttgaaagaaa
    17341 tgatatccct ttttcatgtc aatcgtgtct ttgttggaac agacggtttc aacaaagatc
    17401 ttggctttat gggcaaggac atgatgcgct cagaaggggt gcgttacatg gcagatgctg
    17461 ctgaggaagt ggtgattctg accgattcca gcaagttttc aaaaaccagt ctggtacacc
    17521 agttgtcatt agctgatgtc aatcgcgtca ttacagacca agcactagac aagcaaacac
    17581 aagagttatt aagtgctagt ggtcttgttc ttgattttgt ttcttagaaa ataggtggct
    17641 agatgaaaga agaacgcaga cggctccttg ccaaggtggc ttacttgcat tatgttcaag
    17701 gcaagagcca aaccttgatt tcaaaggaaa tgaatatcta ccgaacaacg gtttgtcgga
    17761 tgttagcaaa agccaaggaa gaaggcattg ttcggattga aattgccgac tatgatgctg
    17821 atttatttgc tttagaagaa tacgtgcgaa agcagtatgg cttggaaaaa ctagaccttg
    17881 tgcccaacca ggttgaagat accccaatgg ataccttgac aaatgttgct aaaacagcag
    17941 ctgaggtttt tagacatgtt gttaaagatg gcgataagat cggtctgtcc tggggagcga
    18001 ctctttcttg cttgatggat gagttaaatc caaaagcgat gaaagatgtg tttatttatc
    18061 ctttggcagg aggacctagc catattaatg ccaaatacca tgtcaatacc ctggtttacc
    18121 gcttggctcg catctttcat ggtaatagcg cttttatgaa cgctatggtc attcaagaag
    18181 acaaacactt ggctaaggga atcttacaat ctaaatactt taacgacatt ttaacaagct
    18241 gggaccagct agatcttgcc ttagtaggaa ttggtgggga gcctaatagc cttgagcaga
    18301 gtcaatggcg tgatttatta accagctcag accatgacca actcaaatat gaaaaagccg
    18361 ttggtgaagt ttgttgccgt ttctttgatc aggcaggtca gccagtttac actggcctac
    18421 aggatcgaac tattggtatt tcgctagaac agttgaggcg tgtccccaaa acaatggctg
    18481 ttgcaacagg taaacacaag gctaaggcaa tattggcagc cttaaaagca ggttttatca
    18541 attatctagt gacggataaa gaaaccatgc tagctgtttt agcgttagat gaagatattg
    18601 atcttaataa cgttcttctt taatagaaat tcaaagatac tatgatattt atagaggccc
    18661 aagaccaaac ggtcagggct ttttttgttt gatgtgctga taaaacagag attgctcaaa
    18721 tgtgcagtta atgtattggc acaaaagtgc attaaccaaa atatagcaca aaatatattg
    18781 accctaaaaa caaaaatgat acaataaact taccaacgaa atgtaaacga ttacaaacat
    18841 aataacatca ccttcgaaaa aaagactcat tgccgttaca tcacatgaaa aagaaaaaaa
    18901 ggagacacca atgcaagtta ttgtaccaga ccaaatcatt atgggcttaa ttttaaatgc
    18961 aggcgatgcc aaacaacata tctaccaagc tctgaaatgt gctaaagagg acgattatgc
    19021 cactagtgaa aaagaaatgg ccttagcaga tgatgcctta ttagaagccc acaatttaca
    19081 gacccagttt cttgcccaag aagctagtgg taataagtca gagattacag ctctttttgt
    19141 tcactcacag gatcacttga tgacgaccat tacagaaatt aatctcatca aagagattat
    19201 tgaccttcgt aaagaattgg caactaaata agttttcatg actaaaaagg agattgacat
    19261 gattaaaatt ggattgtttt gtgcagcagg attttcaacc ggtatgttgg tgaacaacat
    19321 gaaagtagct gctgaaaaaa aaggcattga ttgtcagatt gaagcctatg ctcaagggaa
    19381 attagcggac tatgcaccat tacttgatgt ggcactttta ggcccacagg ttgcctatac
    19441 tctggataaa tcagaagcta tttgtaaaga caatgatatc cctattgcag ttatccaaat
    19501 ggctgattat ggaatgttag acggcaacaa ggtgcttgat ttagccctta gccttgttaa
    19561 agaataggag tcaatcatgg caaaaatgaa tatgcaaaac atcatcaagc caatcatgac
    19621 atttgtgaat atgcgcggca tcattgcctt aaaagacggt atgttagcca ttttaccttt
    19681 gaccgttgtg gggagtcttt tcctgattgc tgggcagatt cctttccaag gggttaatga
    19741 tgccattgct agtgtgtttg gtgctgactg gacagaaccc tttatgcagg tttaccatgg
    19801 aacatttgct attatggggt tgatttcctg ttttgcaatt ggctattctt atgctaaaaa
    19861 ctcagatgtt gaacctctcc cttcaggggt tctatccttg tcagccttct ttattttatt
    19921 gagatcatct tatgttccag cagaaggtga agctattgga gatgctatca gtaaggtttg
    19981 gtttggtggt caagggctta ttggtgctat tgtgattggt ttaacggtcg gagctgttta
    20041 cacaacattt atccgtcgtc atatcgtcat taaaatgcca gatcaagtgc cacaagccat
    20101 cgccaaacag tttgaagcca tgattccagc ctttgtgatc tttaccttgt caatgcttgt
    20161 gtatattatt gccaagtcag taacaggtgg tggcaccttt attgaaatga tttatgatgt
    20221 cattcaggta ccgctgcaag ggttaacggg ttctctttat ggcgcactag gtattgcctt
    20281 ctttatttcc tttctttggt ggtttggcgt gcatgggcaa tctgtggtca atggaattgt
    20341 cactgctctt ctcttatcaa atctagatgc caataaggcc ttgatggcag caggtgagtt
    20401 atccctagac aagggtgccc atattgtaac ccaacaattt ttagactctt tcttgattct
    20461 atcaggttct ggcattacct ttggtttagt ggtagctatg atctttgcgg ctaaatctaa
    20521 acaatataag gccttaggta aggttgcagc cttcccagcc ctctttaatg ttaatgaacc
    20581 agttgtcttt ggttttccaa ttgtaatgaa tccagtcatg ttcttgccat ttatcttggt
    20641 tcctgtcttg gcagctctca cagtttatgg cgccattgct attggtttca tgcagccctt
    20701 tgcaggagtg acccttccgt ggtcaacacc agccattatc tcagggttca tggttggtgg
    20761 ctggcaagga gctattgtgc aaattcttat actcatcatg tcaacgttgg tgtacttccc
    20821 attctttaaa atccaagata atatggctta tcaaaatgaa caagctactg aagagtcatg
    20881 atttagcgtc cacacaaatt cttgagacca tctccagtta gaggtggttt tttagaaaaa
    20941 gaagagcgat ttatcaggcc tatattgcac aatagtgcaa aataaagtga ctatctcttc
    21001 ttatggttaa ggatacgcaa aagtcgcaat atatgataaa atagttacga aagaaacatt
    21061 tataatttcg taagaaagaa ggagaccccg catgactgaa acaaaaagcc cttattttgg
    21121 acatttaaca gacagaatgg ctcactatcg tgaagctgtg ttagacaaga aaccttatat
    21181 tgatgctgag cgtgctattt tagccactga agcctatcaa aaacaccaaa ataagccagc
    21241 aaacctcaaa cgcgcttaca tgcttcaaac tattttggaa aacatgacca tctatattga
    21301 ggatgaaagc ctcattgctg gtaaccaagc ctcttcaaac aaggatgcac cgattttccc
    21361 agaatacact ctggaatttg tcctcaacga acttgatctt tttgaaaaac gagatgggga
    21421 tgttttctac attaccgaag aaaccaagca acagctccga gacattgccc cattctggga
    21481 aaacaataac ttgcgtgctc gctgcggtgt cttgctacca gaagaagtgc aagtgtacat
    21541 ggaaacaggt ttctttggca tggaaggcaa gatgaactct ggggatgccc atttagcggt
    21601 taattaccaa aaactcttgg aacacggctt gaagggattt gaagaacggg cacgagctgc
    21661 caaagctgcg cttgatctga ccattcctga aaacattgac aaataccatt tttacgactc
    21721 tgtttttatt gtcattgatg cagtcaaaac ttatgccaaa cgctatgcca agttggctag
    21781 agaattagca aaaacggcta aaccagaacg tcaagcagaa ttgttagaca tcgcgcgcat
    21841 ttgtgacaaa gtgccttacg aaccagcgaa aacctttgca gaagctgtgc aatctgtctg
    21901 gttcattcag tgtattttgc aaattgaatc aaacgggcat tccctatctt atggccgctt
    21961 tgaccagtac atgtatcctt atgtcaaagc tgatttggag gctggccgtg aaacagaaga
    22021 cacaatcgtt gagcgtttga ccaacctctg gattaagacc ttgaccatta ataaagtacg
    22081 gagccaagcc cacaccttct catcagcagg tagtccactt taccaaaacg tgacgatcgg
    22141 cggtcaaaca cgcgataaaa aagacgcggt caacccattg tcttaccttg tcctacggag
    22201 tgtggctcaa accaaattgc cacaacctaa cttgactgtt cgttaccata aaggcctaga
    22261 caataccttt atgaacgaat gtattgaggt catgaaactt ggttttggta tgccagccat
    22321 gaacaacgat gaaatcatca ttccatcttt catcaaaaaa ggtgtctcag aagaagatgc
    22381 ttatgattac tcagccattg gctgtgtgga aacagcagtt cctggaaaat ggggctaccg
    22441 ttgcacgggg atgagttaca tcaacttccc taaaattttg ctgattacca tgaatgacgg
    22501 gattgaccca gcatcaggca agcgctttgc aaaaggacac ggtcacttca aagaaatgac
    22561 ctcttatgaa gaattaaaag cagcatggga tgccaccctg cgcgaaatta cccgcatgag
    22621 cgtcattgtt gaaaatgcta ttgacctcgg tcttgaacgt gaagtaccag atattctttg
    22681 ctcagccttg acagatgatt gtatcggtcg tggcaagacc ttaaaagaag gcggagctgt
    22741 ttacgactat atctcaggcc ttcaagtcgg tattgccaac ctatcagact ccctagcagc
    22801 gctgaaaaaa ttagtgtttg aagaaggtca attaactcca gaagaactct ggcacgccct
    22861 tgaaagtgac tttgctggag aacgcggaga agacatccgc caaatgctca tcaatgacgc
    22921 accaaaatat ggtaatgatg atgactacgc agatagttta gtcgtcgaag cttacgacac
    22981 ctacattgat gaaattgcca aatacccaaa tacccggtat ggccgtggcc caatcggagg
    23041 tatccgctat tctgggacat catcaatctc agccaatgtc ggacaaggaa aagggacctt
    23101 ggcgacacca gacggtcgcc atgcagggac acccctagca gaaggctgtt caccagaaca
    23161 cagcatggac aaaaaaggcc ctacttcagt attgaaatct gttgctaaat taccaacaga
    23221 tgagattgtc ggaggagtac tactcaacca aaaagttaac ccacaaaccc tggccaaaga
    23281 agaagacaaa ctgaaattaa tggccttact tcgtaccttc tttaatcgcc tacacggtta
    23341 tcatatccag tacaatgtcg tgtcacgtga aaccttgatt gatgcccaaa aacatcctga
    23401 aaagcaccgc gacctcattg tccgtgttgc cggctactca gctttcttca atgtgctctc
    23461 aaaagcaacc caagatgaca ttattgaacg tacagaacac acactttagg agaaaataag
    23521 tatggaatac atgttagaca ctttagattt agaagcgatt aaaaaatggc atcacatttt
    23581 gccgctcgca ggtgtgacct caaacccttc tattgccaaa aaagaaggtg acattgattt
    23641 ctttgaacgg attcgagaag tccgtgctat tatcggtgac aaggcttcta ttcatgttca
    23701 agtgatcgcc caagattaca aaggcatttt aaaagatgct gctgaaatcc gcaggcaatg
    23761 tggagatagt gtgtatgtca aagtacctgt aaccacagag ggccttgcag ccattaaaac
    23821 cttgaaagcc gaaggctatc atatcacagc aaccgctatt tatacgacct tccaaggact
    23881 attagccatt gaagctggcg ctgattattt ggctccttac tataaccgta tggaaaatct
    23941 taatattgat ccagaagctg tcataggaca attagcagaa gctattaacc gcgagaatgc
    24001 tgatagtaag attttggcag caagctttaa aaatgtggct caggtcaata aatcctttgc
    24061 tctgggagca caagccatca ctgctggccc agatgtgttt gaagcaggat ttgccatgcc
    24121 atctattcaa aaagcggttg acgattttgg caaggattgg gaagccattc atcaccgcaa
    24181 gagcatttaa gaaatagcat cagtagtaat cacatcatta ggaatcacag ataaaggagt
    24241 tcaacgatga aagtatttgc aagtccatct cgttacattc aaggtaaaaa tgccttgttt
    24301 accaatgcta aaaccctaaa acaactaggg gatagtccca ttttactgtg tgatgacgtg
    24361 gtgtatggca tcgtcggaga aagatttgaa agctatttga tagataatag catgactcct
    24421 gttcacgtag cctttaatgg cgaagcctca gataatgaaa tcagtcgcgt ggttgccatt
    24481 gccaaggaaa atggcaatga tgttattatt ggtcttggtg gtggaaaaac cattgacagt
    24541 gctaaggcta tcgctgattt acttgctgtt ccggtgatta ttgccccaac cattgcttca
    24601 acagatgccc caacctcagc cttatcggtt atttacaccg atgaaggagc ctttgaaaaa
    24661 tacattttct attcaaaaaa tccagatctt gttttggttg acacacaagt gatctgtcag
    24721 gcaccaaaac gcttattggc gtcagggatt gcagatgggt tagcaacatg ggtagaggcg
    24781 cgtgctgtta tgcaaaaaaa tggagacacc atggcaggtg gcaatcaaac cttggcagga
    24841 gttgccattg ccaaagcctg tgagcagacc ttgtttgcag atggcctaaa agcgatggct
    24901 agctgtgaca gacaagtcgt gaccccggcc ttggaaaatg tcattgaagc caatacactt
    24961 cttagtgggc ttggttttga aagtgcgggt ctggctgctg cacatgccat tcacaatggc
    25021 tttacagcgc tgacaggtgc tattcatcac cttacccatg gtgaaaaagt cgcctatggc
    25081 acgttgaccc aactcttctt ggaaaatcgt tcgcgtgaag aaattgaccg ctacatcgac
    25141 ttttaccaag ctattggcat gccaacaacc ctcaaagaaa tgcatttaga cacggctact
    25201 caagaggatt tcttaaaaat tggccgtcaa gcaaccatgg caggcgaaac catccatcaa
    25261 atgccatttg ttatcagtcc agaagatgta gcagcggctt tggttgctgt tgatgcctat
    25321 gttacgagtc gataggctaa ccaaaaagag atttcttatt tgccagataa cctcaaaaaa
    25381 acaagtctag ggagtcactt cctagacttg ttttgcttat tgttggttgg taatagcact
    25441 tttagcgggg tcattgtggg caatacggct ggcagctgct gccgcaatgg cgctaacaat
    25501 atcatctaaa aaggtatggc aggagtagcc atctttgtgg ttgagtttgt caacaattcc
    25561 tggttttgtc ttgtccagat acccataatt ggtaaaaccg atagacccat agaggttaac
    25621 aatggataag gccaggattt catcaatccc atacagccct tggtcggttt tcaagatact
    25681 aaggagtggc tcagacagct ggttggcttc agccaattta tccaattcta ccccagtgat
    25741 aatagcattt tgcacttctc gttttgcaag caccgcctca acgctctcta aacattctgc
    25801 catggtcaaa ttgggaatat aatcattctg caaaaaaagc accagctctg caatagcttc
    25861 taaagaaccc cctcgctcag ccagtaattg ataagacact tctcgtaggt gatggtcagt
    25921 tgacatgtga gacctccttt ttttctttat tgtaacataa aagatttcga gaaacctcta
    25981 caacagctaa agaggggata gttctttttt aagagatgtt gactactgag atcccctact
    26041 tggtaaaagt caaagaaacg atagtaaagt aaccatctta cctaggccta aatacaatca
    26101 ccacccctag gtgacacaca gcactatttc aaaaatgata ttagtatggc aaaacaaaaa
    26161 gacaacgcct tcttttttct ccttactatc tcctttaatt ttcatatttt ttaaaaaaac
    26221 tattgataaa ctagttaagt aagcgtatac tatggttagt gagcgaaatt agaaaagagg
    26281 acaagcatat gaatctactt ggatcaagac gggttttttc taaaaaatgt cggctagtaa
    26341 aattttcaat ggtagctctt gtatcagcca caatggctgt aacaacagtc acacttgaaa
    26401 atactgcact ggcacgacaa acacaggtct caaatgatgt tgttctaaat gatggcgcaa
    26461 gcaagtacct aaacgaagca ttagcttgga cattcaatga cagtcccaac tattacaaaa
    26521 ccttaggtac tagtcagatc actccagcac tctttcctaa agcaggagat attctctata
    26581 gcaaattaga tgagttagga aggacgcgta ctgctagagg tacattgact tatgccaatg
    26641 ttgaaggtag ctacggtgtt agacaatctt tcggtaaaaa tcaaaacccc gcaggctgga
    26701 ctggaaaccc taatcatgtc aaatataaaa ttgaatggtt aaatggtcta tcttatgtcg
    26761 gagatttctg gaatagaagt catctcattg cagatagtct cggtggagat gcactcagag
    26821 tcaatgccgt tacaggaaca cgtacccaaa atgtaggagg tcgtgaccaa aaaggcggca
    26881 tgcgctatac cgaacaaaga gctcaagaat ggttagaagc aaatcgtgat ggctatcttt
    26941 attatgaagc cgctccaatc tacaacgcag acgagttgat tccaagagct gtcgtggtat
    27001 caatgcaatc ttctgataat accatcaacg agaaagtatt agtttacaac acagctaatg
    27061 gctacaccat taactaccat aacggtacac ctactcagaa ataataccaa aaggctagac
    27121 ctctgctcac taggcctagc tttttacatc aaaaaaagca atgactatag aaagtaaaaa
    27181 tactagaaaa agcaatgatt gccgtcattg ctttttatga tttgtcaaaa agtaaaaagc
    27241 aaattgaaaa aatcgccctg gagctgttga gataaactac accatttatt aagataccaa
    27301 atcaaaaagc taacaccata agagcgttag ctttttaaag agcctcagga cagtttatgt
    27361 ttaatggctt ctaggtaggt ttgaaacata tgatggatcg ttttgcaatt aagtaattgg
    27421 aagatctcca agatctgcac acacttttgc tcagccccct ctttaccatt tatgagacca
    27481 ataatgccat caaaaaaaga cacacaacac cgttcatata agcaatcatc atttagctga
    27541 taatcttcaa tttttgccaa aatctcagaa gctttatcat actcttgtcg ctgaataaac
    27601 aaaatcaaca tattgacaaa catccgaatc gattcattcc cataatacct tagggtattg
    27661 tatttatcga ggttcaaaat aacttttgaa aacaccatct caataaagca agactcaaaa
    27721 ataaacatac aattattaaa aagcacagtc tcatagtgac tccaagtttc aatattaata
    27781 agatagtttg acaaataagt ccgctctgtc agacagtctt cctcagtaag agttgctaat
    27841 aacaccttga ccaaggcaaa aaggttcttt tcctttgttg acttactatc tttataagaa
    27901 tcaaggatat gatttaaacc ttctatgttt cggcattcaa aataatgttt tgccgtatcc
    27961 atatcaataa attctttgta ttgtttaaag ttattactaa taaagagaaa ctcatcaaca
    28021 ttgacattca agttgtctaa gatataaagt aactttttcg aagaaatgtc aagattattt
    28081 ttaataaaac gataataggt ttgcctagtg agataatcac cacaaacttg tttaatcgaa
    28141 atgttttttg aatgcctaat gaattcaacg gtttcaccaa tttccatatg tcaagccttc
    28201 ctagttgatg tcaaaaatac gttacgcatg tacctaatac gtaacaagtt gaatgtttcg
    28261 gatgatagtc gcttatgata ggtgcataag gtcaataacc agatgcgata gttttatcaa
    28321 ctgtcatatg ttaaaccttt ctaatctaat agatgttaaa aatacgttac gtgtgtacct
    28381 aatacgtaac aaaataatgg gttagcaaaa taagcagcta tgatatagcc ataaggttaa
    28441 aaggaggcgc ctactatgtg gttattgtta ctatttttgt agttcctttt gcaaaatggt
    28501 agtagataaa aatgagaagt caataaaagc attgacaaac aaaagaatca ttttgtttat
    28561 attatcagta tttttaagtt tcttgtcaac tgaaatgagc atctactagc cacaatagta
    28621 actaaaaggc taattgtcag taaaaatgag ataaatcagc caggaaaaag cctacgagtc
    28681 aactaatgga caacccaaaa aaagacaaag gactttcact tggtgttgtg gaccctatca
    28741 cgatccttat cacagaatca gttattaaca aaatgagaac ggtcccatat catctaaaaa
    28801 aactgtcaag cacaacctat tgtttgctag ctgatcttgc ctatcagtct gcctagcaga
    28861 tacggcagaa gataagaaat caaccaaaag atgataagtg caacgactca tagcgtcttt
    28921 tgaccaaaac caattaggct cacatgagat gagaataatt gggttgggtt gtcagtgtca
    28981 actaaccgtg ttattgtcta ttaccattca tggtatcagc gacatcgtat gataatcata
    29041 cgattcagct aagtaaggag gtgtgtccaa tgtaccgtta aaagcaaatg cagtagatta
    29101 acttattttg aaagaggtat aaaaaaaatg aataaaaaga aattaggtat cagattatta
    29161 agtcttttag cattaggtgg atttgttctt gctaacccag tatttgccga tcaaaacttt
    29221 gctcgtaacg aaaaagaagc aaaagatagc gctatcacat ttatccaaaa atcagcagct
    29281 atcaaagcag gtgcacgaag cgcagaagat attaagcttg acaaagttaa cttaggtgga
    29341 gaactttctg gctctaatat gtatgtttac aatatttcta ctggaggatt tgttatcgtt
    29401 tcaggagata aacgttctcc agaaattcta ggatactcta ccagcggatc atttgacgtt
    29461 aacggtaaag aaaacattgc ttccttcatg gaaagttatg tcgaacaaat caaagaaaac
    29521 aaaaaattag acactactta tgctggtacc gctgagatta aacaaccagt tgttaaatct
    29581 ctccttgatt caaaaggcat tcattacaac caaggtaacc cttacaacct attgacacct
    29641 gttattgaaa aagtaaaacc aggtgaacaa tcttttgtag gtcaacatgc agctacagga
    29701 tgtgttgcta ctgcaactgc tcaaattatg aaatatcata attaccctaa taaagggttg
    29761 aaagactaca cttacacact aagctcaaat aacccatatt tcaaccatcc taagaacttg
    29821 tttgcagcta tctctactag acaatacaac tggaacaaca tcttacctac ttatagcgga
    29881 agagaatcta acgttcaaaa aatggcgatt tcagaattga tggctgatgt tggtatttca
    29941 gtagacatgg attatggtcc atctagtggt tctgcaggta gctctcgtgt tcaaagagcc
    30001 ttgaaagaaa actttggcta caaccaatct gttcaccaaa tcaaccgtgg cgactttagc
    30061 aaacaagatt gggaagcaca aattgacaaa gaattatctc aaaaccaacc agtatactac
    30121 caaggtgtcg gtaaagtagg cggacatgcc tttgttatcg atggtgctga cggacgtaac
    30181 ttctaccatg ttaactgggg ttggggtgga gtctctgacg gcttcttccg tcttgacgca
    30241 ctaaaccctt cagctcttgg tactggtggc ggcgcaggcg gcttcaacgg ttaccaaagt
    30301 gctgttgtag gcatcaaacc ttagtatgga aatgcatttc gttagaacag aacctgaggc
    30361 acgccgcata gctgaaacct tttgtgccga aaacacacaa acaaaaaccc ctatgcgcgt
    30421 gcagcagtta agctatccat cagacacaga tcactcaggt ggtgagctat acatctatgc
    30481 tttgtctcct gctggattta tcatcgtatc aggagatacc agagcgcaca ccattttagg
    30541 ctattctttt gataataacc tggacctcaa ccatgataat gtcagaagta tggtagaagc
    30601 ttaccaaaaa caaatcaact ctttggattg atcggtcaaa gtagcttttg ccccctcacc
    30661 actcgtttaa gacgaccgtg tttctaaaat accactacct tttaagtttc acgccggcct
    30721 aagcaagtgt ttgtctcatg gagcagactg tttccatcga aaagtcaccc attgataaag
    30781 gaggtcatca acgcctacta atgatgactt ccctaagatg ccaaggtaaa ggactgagtt
    30841 ctgtagagat gggactcagt ccttttacga tttttgggag ttattctagc cataccgtgg
    30901 caagaaaaca acactcaaaa atagccaacg tcagtttctg ttcatacatg actacagcca
    30961 aacaccacaa cctaacctat cctaaacaca gtggctttct aacgaacagt cacaacgagc
    31021 agcaacacca aaaagtaaga ttaagctacg tttaagacta atgatataag ataacagtaa
    31081 ttattaatga gaaaagaggc tcaaaaggac atgaaacaaa tgaataaact cattacagga
    31141 gtggtaacgc tggcgacagt cgtgacctta tcagcttgtc aatcatcaca caacaacacc
    31201 aaactcgtct caatgaaagg agacaccatc actgttagtg acttctacaa tgagaccaaa
    31261 aacacagaac tcgcacaaaa agccatgtta agcttggtga ttagccgcgt ttttgagaca
    31321 caatatgcca acaaagtctc tgacaaagag gttgaaaaag cctataaaca aaccgcagac
    31381 caatacggta catcctttaa gacagtccta gcacaatcag gcttaacacc agaaacctat
    31441 aaaaaacaaa ttcgcctcac aaaattagtc gaatatgccg tcaaagaaca agccaaaaac
    31501 gaaaccatct caaaaaaaga ctaccgtcag gcctatgacg cttatacccc aaccatgacc
    31561 gcagaaatca tgcagtttga aaaagaagag gatgccaaag cagcacttga agctgtcaaa
    31621 gctgaagggg cagactttgc agctattgcc aaagaaaaaa ccattgcagc cgataaaaaa
    31681 acaacctaca cgtttgactc aggcgaaaca accctaccag cagaagtagt tagagctgca
    31741 tcaggcctca aagaagggaa cagatcagaa atcatcacag cgcttgatcc agccacctca
    31801 aaacgcacct accatatcat caaagtcacc aaaaaagcaa ctaaaaaagc agactggaaa
    31861 gcgtaccaaa aacgcttgaa agacatcatc gtgactggca aattaaaaga ccctgacttc
    31921 caaaacaaag tcatcgctaa agctcttgat aaagcaaatg tcaaaatcaa agacaaagca
    31981 tttgccaata tcttagccca gtttgcaaaa cctaaccaaa aacaacctgc ccaaaaatag
    32041 agggttgcca gtttatttcc ctaaggttgc ttaaaaagac agccctagcc atcactcccc
    32101 ttttcaaaac accgataatg gaaacgtgtc ctatactgat ggtctagggc aatgtcataa
    32161 agctaaagac tctatcccaa taaggataga gtctttaatg ttttaaaacc agtcagcaaa
    32221 aaaattcacg ctaagtctag aatgaggctt agcgtgaatg gtatcagtga tagtccgtta
    32281 aactagctga ttgggagtca gaaactctcc ttacccaatc tgactatacc cccagtacca
    32341 gaggtgtctc atcgcagaag tagcaatcag ataatcgtct gataagctct tgtcctcaag
    32401 caactgatga gtctcgtcgc acgggccatg agctgccggt ggctgatagc cagtaggctc
    32461 atcagccaaa acttttttgg agtaacgctt tggtctcaga cggcgccaga gggttgagag
    32521 aggggtgaga aaaaaacgat ggatcatctt ggcctccttg tctaatgata gtcaccttaa
    32581 cagacaaaag tgtcaataat gtcattttaa gaagaaaaag attaaaaatc aaggtcacaa
    32641 actttccttt gttaaaaaaa cgagacccta acaaagaccc taaatacccc tcgcacctga
    32701 gcttctcctt gcgcagctat cttgcgcact cagttgtctc caggctagtg atagcgatac
    32761 cattattgtc ctataggcta tcgcgacaac cccttttttc gcttcaacga ggccgacaca
    32821 gctgtcatga cagaaaaaat acctctgcct atctaaggga ccattaattt ttctctcacc
    32881 aaaaaactga ttttagaaac gaaaaagcat ggtgtataat aaagttcgga acaattatga
    32941 cataataatg aaagtaaggt taactaaaca ttgacaaatt gtaagtataa acttagaaag
    33001 ttatctgtag ggctcgtctc cgtcggaacg atgctgatag ctccgacagt tttaggacag
    33061 gaggttagta ctggtgctag cagtactgag acgagtgcta gtactaatac tagtaccgct
    33121 agcgctggta ccagtacgag tgagacagct gccagcggaa ctgggagtga agcagctgta
    33181 gtatctagcg aaggaagtca gagttcagaa tctggacaag cctcaacaca acctcaagca
    33241 cagactttag aacaatcagc agcaacgtcg ccatcatcga actcttctac tagtagtagt
    33301 gaagataaag ctcctaaggc agcaagcact aaatcatctt cagcaactgt ggctagctct
    33361 agtaatggta gcaatcaagg tgctggtgct gaagatgcac cacagatgat ggacgtggaa
    33421 cggtatacag ttgataggga aaaaacagag ctaaatatta aagacggtaa gactccaaaa
    33481 actaggaata gtgtcgataa agatacaaag cttattagaa accgcgatgg caaacagcgt
    33541 gatattgttg atatcaagcg tgaagtaaaa gataatggcg acggaacctt agatgtaacc
    33601 ttaaaagtaa cacctaaaga aattgataaa ggtgccgatg ttatggccct tttagatgtc
    33661 tctaaaaaga tgacggatgc tgattttaaa aacgctaagg ataagatcaa gaaattagtc
    33721 acaaccttaa cgagtaaatc agcgagtaac tcagataatg atgagcataa acataattct
    33781 cgaaattcgg ttcgtctgat gaccttttac cgtgagatta gcaacccaat tgatatatcg
    33841 ggaaaaactg atgctgaact tgataaatta ttagacgatc ttcgagtaaa agctaaagct
    33901 aattatgact ggggggttga tttacaaggc gctatccaca aagctcggga aatttttaat
    33961 aaggaaaaag agtcaaaaaa acgccagcat atcgtcttgt tctctcaagg cgagtcgact
    34021 tttagttatg atattcaaaa gagtgagaaa gagaaaagta gcaatttatc tcgtataaac
    34081 gaaaaaatta cctcctctaa tccgctgttg ccctggccac caatttttga tcatacgcat
    34141 caaaatggag atatgattaa tgatgttaga gctttaattg catttgctag taagctaggt
    34201 ataaagaatt tatcatggat tgaaacatca cttagtgcgt taagcgtagg aagtaacttg
    34261 cttggttctg tttttggtgg aggtggacta ggcgagtacc taactttaaa agaatatgat
    34321 tcacaaaaat taaatgaaga ccaatttgat tatactaaac gcgtaggcga aggatatcat
    34381 tatcatagtt tttcaaatag ggaatctgag gataaaatgc ctcttgaaac agaaattaaa
    34441 aaagctttag aagctgcttt accaaaattc gaagaaaata actggtttac caaagtacta
    34501 aaatactttg gattgaaaga taaagctgaa caagccaaac ttgatgtaat catgaaagtg
    34561 attaaaagtg tcttctacaa gcgccagtac cactattata atcacaatct ctcagcgata
    34621 gccgaggcta agatggcgca gaaagagggc attaccttct attccgttga tgttactgct
    34681 ttaaacaccg ctagaagagt gaagcgacaa acagcagtgc taaagaacac taaggaagaa
    34741 gaacgtaata agaagtttga tgagtatctg aaaaagatgt ctgaaggcgg taacgcattt
    34801 tttaacgatg tggataaggc agataagttc aaagacactt taacaaaact tacgattaaa
    34861 gacgagtttg acggcaaggt tagtgtcgat aaaacttcat atcaatccag tagtgcaatc
    34921 aactattctg aagctagtaa tagtttttgg cgtactacca aagaaagtct cacttggacc
    34981 atttccaaag agcagttgaa aaaagccttt gaagatggaa aaccgttaac cttcacctat
    35041 aagcttaaag ttgataacaa caagtttaaa acagctctcg aggaaaataa taagaagaag
    35101 agaacaaaac gttctacacc tacagaaaat gaaaactctg tcacagaaaa aatcatttca
    35161 aatactacta cctacgaaat taatgaacaa aaagttgaag ggaacaaact tggtgatgtt
    35221 agtctgacat acagtaaatt caaggttcct gtaccacaga ttgatgggca tgttattgag
    35281 ccacaagcac cgacattacc taagttacct cctgttattg agcatggccc taactttgag
    35341 tatgaggaag aaacaggtta tcagttacca cttaaacatg gcagcaatgc accagacaca
    35401 caagtgacaa ttgaagaaga cacagttcct caacgtccag atatccttgt aggcggtcag
    35461 agtggaccgg ttgacatcac cgaagatacc caaccaggta tgtcaggctc aaatgacgcg
    35521 acagttgtcg aggaagacac agcacctaaa cgtccagatg tccttgttgg tggtcaaagt
    35581 gagccaatcg atatcactga agatacccaa ccaagtgtgt caggctcaaa tgacgcgaca
    35641 gttgtcgagg aagacacagt acctcaacgt ccagatatcc ttgttggcgg tcaaagtgat
    35701 ccaatcgata tcaccgaaga tacccaacca ggtatgtcag gctcaaatga cgctactgtt
    35761 atcgaagaag atacgaaacc aaaacgcttc ttccactttg ataacgagcc acaagcacca
    35821 gaaaaaccta aagagcaacc atctctcagc ttaccacaag ctccagtcta taaggcagct
    35881 catcacttgc ctgcatctgg agacaaacgt gaagcatcct ttacaattgt tgctctaaca
    35941 attattggag ctgcaggttt gctcagcaaa aaacgtcgcg acaccgaaga aaactaaccc
    36001 tcgttagtct agcgacccta agcctttggc tttaaaaatt tctcattatc cccctcaaac
    36061 accggtactt cactgagatg gcgctttaaa aaggcgctag tctcaaatca ggtatcccct
    36121 acggttgagg gggattaatg ggtatcacaa caagtaaaaa ggtataatga tgaaatatga
    36181 ctaaaaaatt tattaaatca gcagtgattc taggcttagc aagcataacc atcagcatga
    36241 cgcaacccgt tgaagccatt tcaaatcgct tagaatatca agaacaatat aaacgtgtca
    36301 aagaagaact agacgcatta aaagaaaaga gcccaaaatt aggagaacgt tccctttatt
    36361 ttagattggt gaatagattt ctaggttggt ttggcgtagt ctgggaccca ctcgcaatca
    36421 aagaaaccga tcacctgttg gacctcatcg gacaagtcaa ttacttggtg agtaacaccg
    36481 agtttcaagc ccctgagtat caaaaggcta aaaacaaact agacttagct gtagcaggct
    36541 atgacctcta cctagctaga gagaaaaaac gtggtgatgc tcctgattac caagtgaccc
    36601 agctaacgct aggacagctc gaaactatca aaacgaagat acaagaggcc ctgaaaacat
    36661 ttgcacaaga ggtcaaaaca atccaacaaa aaaccccaga cctatttccc catgcttatg
    36721 ttttaaacgc taagaccagt gctatcgatg gtaaagcgca acccttttta gtgaatggtt
    36781 actacgatgt cttttataag cagtttttat tcactaataa aaatgacggg tataactata
    36841 ataaagaagc agacaatctg acctcgtatc aaaatttgat taaagagcta ttagacttaa
    36901 aagctgagat cgatcggtta acaaaagacg atcctaaaaa cgatcgttta gagacgttaa
    36961 tgtggcaatc aaagttcacc ttatctgact ttcggagaac ttcacgcctt agcgcctact
    37021 atacacaagg cgattatgtc aaaacggtgg tcctatctga agaagctccc aacttgagaa
    37081 cagcaaaaga aaaggtcaag accttaaaag ggatcttgca tgattactat gttgaaacta
    37141 ataagccaga aaaagctaaa gagtatcgtg tcgaggacga gaccttgccg tctcaacctg
    37201 aggctcctgt tacacctgaa gctcctgctc ctagtcctgt tcctggtcaa aaaccagcag
    37261 aagctgagag atcgtcacaa gctacggagc cagctaaccc gtcaaaagag aattcctcaa
    37321 cagacgcatc tcagggatct cacgatagtg aaaacccagc gacagacagt ccttctcaac
    37381 ctcaggcacc agaccaaggc aatcaccaaa gccaagtgcc taacgacaag cctcaaacgg
    37441 acaagacgga cactcctaac gtcccagccc ctccacagaa cactcctaac gccccagcca
    37501 tcccacagga caccccaaaa gtgcctgagg ctggtggtca gagtggcccc gctgggaacg
    37561 ctgaggagaa agctcctgac tctggaccta aagcgtctga tcaaagctcg tctaaagagt
    37621 ccccatccgt tggcgaagac acgaccccta atcagccaga tgtccttgta ggtggtcaaa
    37681 gtgagccaat tgatatcacc gaagacacaa tcactgacgc tcctccgaca gtatctgggc
    37741 acaacgcatc aacacagccc caatctgtgg tcgaagacac ggcacctcag cgcccagatg
    37801 ttcttgtagg tggtcaaagc gagccaatcg atatcaccca agatacccaa ccgggcatgt
    37861 caggctctaa tgacgctacc gttatcaatg aagatacaaa accaaaacgc gtcttccact
    37921 ttgacaacaa agagccacaa gcatcagaaa aagcggcaga gcaaaaactt gctcctcatg
    37981 atagccacac aactcctcaa gcttcagatg atacagcagc ccctcacttg cctgcatctg
    38041 gagataagcc tgaaaaagcg tttgcacttg ctgccttgac aatggtcgca gctgcagggc
    38101 tactcagcaa aaaacgtcgc aacaccgaca aaaactagtc gctgttagac acaacgcatc
    38161 actaaggcca gtgcatttgc actggcctta gttaggtttt tatcaaatag aggttttttc
    38221 atgacaattg ctagcgtttc ggtgtaaaat aaaggtaata accagcaaag gagcaaccca
    38281 tgacaaagca agatcagtta attgttgaaa aaatggaaca aacctatgag acctttagcc
    38341 caaagctagc aaatctcata gaagccctag atgcctttaa agagcattat gaggaatatg
    38401 ccacacttag aaatttttac agtagtgacg aatggtttcg gcttgcaaac caaccttggg
    38461 atgatatccc gtgcggtgtc ctatctgaag acctcctctt tgatatgatc ggtgaccata
    38521 accaactact agctgatatc ttagacttgg cccctatcat gtacaagcat atgtaaccat
    38581 cgctaacgtc acagatacta cgatgacagg acaaaaaaag cttgaaaatc tcaaaaaaaa
    38641 acagataaaa aacagataat cgttagaaaa acagtagatg cgtctgttaa actaaaggta
    38701 tcaaaacaaa aggagcattt catcatgaaa ttcaaaaaag tattagtcat tcctgcatta
    38761 gcattagcag caacatgctt tttaacagct tgtggcacaa aaaaagattc taaaaaagaa
    38821 gaagttaaag aaatcaaaat gtctgacatc aaagacgacg cagttagcaa aaaaacaaaa
    38881 gtggttgatg gcgaagaagt tacagaatac accacaaaag atggaaacgt gattcagatt
    38941 cctgcaggaa acgaagaagg catggaatca aaagatgctg gtggatcagg cgctcctgct
    39001 aaaaactaag ggttacaaca aagagcataa ggtgactcgc aaacgttacg catgacatga
    39061 ggcagtctgc ctaagatggt aactcactta tcataatgcc taaatagcca aacacactaa
    39121 ctcgcaaatg tacaaaatca tatacccgtg cttatgtgag cgaacggtta tatgattttt
    39181 gttttccaaa aacaagaaaa gaacgctaaa atagccagtt tgtcgtgttt caagctacga
    39241 aaaaggaagt gctaaaatga aaaaaagatc cattatacct gtcatcttac tgttggtcat
    39301 cggattaatc gttggtgtta agcgtcatca tgactagtag gtcttgctag tgaccgttca
    39361 aaagggataa cgttggtatg gtataaccat aaaaatcgta taaaaagaaa aaatagctat
    39421 tgaggagaaa gtatgtttca gttaagaaaa aaaatgacgc gcaaacaatt agccttgttg
    39481 agtgctggag tgttgacctg tgtggttggt ggtagctact tgataatgaa ccatcaacaa
    39541 caagaaattg tctctagtgt caacaaagta aaagccttaa ccataaaaga agccatggaa
    39601 caaggaaaag atatcagctt gaccttagct ggcgaagtaa cagctaacaa cagcagcaaa
    39661 gtcaaaatcg actcaagtaa aggagaagtc aaagatgtct ttgtcaaaaa aggcgatgtt
    39721 gtcaaagtag gacaaccctt gtttagctat gaaacgtcac aacggttaac ggctcaaagt
    39781 tcagaatttg atgttcaaac caaagccaat caactccaag ttgctaaaac caatgcagca
    39841 ttgaagtggg aaacctacaa tcgcaaggtc aatgaaatca acaccctaaa atctcgctac
    39901 aacactgcac cagatgagag cttactagag caaattcgca gcgcagaaga cagtgtatcc
    39961 caagcactaa gcgatgccaa aacagcagat agcgatgtca aaaccgctca aatcgaactc
    40021 gataaagcta atgctactgc cgcaacggaa aaaggtaaac tagagtatga caccgttaag
    40081 tcagacaccg caggaaccat tgttagtcta aatactgatt tgccaaatca atcaaaatcc
    40141 aaaaaagaaa atgaaacttt tatggaaatt atcgacaaat caaaaatgtt agtcaaaggt
    40201 aacatcagtg aatttgaccg tgacaagtta aaaatcggtc aaaaagtcga agtgattgac
    40261 cgcaaagaca actctaaaaa atggactgga aaagtaaccc aagttggcaa cctcaaagca
    40321 gaggaaaaag gccaaggtca aggccaaggt ggcaatgacc aacaagataa tccaaaccaa
    40381 gcaaaattcc cttatgttat cgaacttgac caatcagaca agcagccact cattggttca
    40441 cacacctatg ttaatgtgct caacaatgtt ccagaagctg gcaagatcgt attgaaagaa
    40501 acctttacaa tggcagaaaa tggaaaaacc tatgtgtgga aagttgataa aaacaaggtc
    40561 aaaaaacaag aaatcaagac taagcccttc tcaaaaggtt atgttgaggt gacaagtggc
    40621 ttgactatgc aagataagat tgctcagccg cttcctggca tgaaagacgg tatggaggta
    40681 ggaagtattg ttaaacctta aagatattcg aaaaagctat catcttggga ctgaagaatt
    40741 tgcgatttta aaaggaatcg atttagaagt taacgagggt gactttttag ccatcatggg
    40801 accatcaggt tcgggaaagt caacattgat gaatatcatt gggtgtttag ataagcctgg
    40861 ctctggctca tatgccattg aaggcagaga cgtgtcatcc ttatctgata atgaacttgc
    40921 tgatttgcgt aatcaaaaaa tcggctttgt ttttcaaaac tttaacctga tgcccaagct
    40981 aacagcttgt caaaatgtcg aattgccctt gacttatatg aatgttccta aaaaagagcg
    41041 tcgcaaacga gccctagaga tgttaaagct agtaggatta gaagaacgta gtgaatttaa
    41101 accgatggag ctatctggtg ggcaaaaaca gcgtgtagcg attgcaagag ctttagtcac
    41161 taacccgagt tttatccttg gtgatgagcc aacaggtgca ctagacacaa aaaccagcgt
    41221 ccaaatcatg gacctattta aacaattcaa tgataacggc aaaacgatta ttatcatcac
    41281 acacgagcct gaagtagctg ctttatgcaa aaagacggtg atcctaagag atggtaatat
    41341 agaacattcc gatatagagt aaagggggaa aataatggaa gatattattg cagctttaag
    41401 ctctatatta tcacacaaga tgcggtcaat tttgacaatg ctagggatta ttatcggtat
    41461 tggtgccatt atcgcaatct tttcaatcat cgaaggtaat accgaaaata ccaaaagaca
    41521 gttgattggt ggcagcaata acactattaa cattgtgttt aacaaaaaaa gtagtattga
    41581 ccctaaattt ccagacaagt caaatgctaa aaaaccagac tatcttcctt ttatggctga
    41641 agaagagctt agcaaaatac agcaagtcaa aggcgtcaaa aatgccctga tctcatatgg
    41701 tattgatgac aaagtctacc atctaggcca aaaatcatct gcaaagattt ctgccatcac
    41761 aaaaaacgta gctgaagtga gacgcatgac atttattaaa ggcagtgact tttcagacaa
    41821 agactttatt gaccaaaaac aggtgattta ccttgaaaaa agcttgtatg agtcactatt
    41881 tccaaaagac gatggccttg gaaaatttgt cgaagtgatg ggaaatccct tcagggtcat
    41941 tggggtgttt gaatcaaaag aacaaagtgg cctcacaagt gggactgaga aaattgctta
    42001 tatcccgcta catcagtggt ataatattaa tggtgtagtg gatgcgacac ctgagatcac
    42061 gatccagacc tatcgcgcag atgacctcaa acctgtagca aagcgtgtca gtgatatgct
    42121 caatcaaacg atccctaaat cagattatat gtttggtgtc atgaacctta aagaatttga
    42181 acgccaacta gacaacctaa acaaatctaa ttttgtgtta ttagctggta ttgctagtat
    42241 ctctcttatt gtaggtggta ttggtgtgat gaacattatg ctagtatcag ttaccgaaag
    42301 aacgagagaa attgggatca aaaaagcgct tggcgctaga cgtaagctta ttttaaaaca
    42361 atttttaatt gaagcggtca ttctaacctt gctaggtggt gtgatagggg ttatttcagg
    42421 gatggtatca ggattgatca tcacaagatc actcgaatat ccctatatcc tatccttgtt
    42481 ctcagttgtc ttgagtttag cgttttgttg tattataggg attgtctttg gactattacc
    42541 agccattaaa gcgtctaagc tagacccaat tgaagcattg cgatttgaat aatcaaccaa
    42601 acaagcataa aagtcaaaaa ggatgaccct ttgatgtcat ccttagctgc ttgatacact
    42661 aaacgccaat ctagaaaggg aagttatgtt taaaatatta gtagttgaag atgatgatac
    42721 catcagtcaa gtgatttgtg agtttttaaa agcgaataac tatgatccag attgtgtatt
    42781 tgatggccaa gctgccttag acaagtggca gacaacgtct tatgatctga ttattttaga
    42841 catcatgctc ccatccttaa gtggcttaga agtgctaaaa acaattcgaa agacatcaga
    42901 tgtccctatt attatgctaa cggcattaga tgacgagtac acacagcttg taagcttcaa
    42961 ccatttgatt agtgattatg tgactaaacc attttcacca ctcattttaa tcaaacgtat
    43021 cgaaaatgta ttacgcgtct ctactccaga tgaaaagcgc caaatcggag acttacttgt
    43081 tgatgagacc gaacatagcg tctattggca aggaacattg gtcaaactaa ccaaaaaaga
    43141 gtatgacatt attgattatt tagcaaaacg tcaccaaaag attgtcacca gagaccaatt
    43201 gatggatgac atatggggct attctgaact ggacacgcgt gtccttgaca accacatcaa
    43261 gaaccttcgt aaaaaaatga cgggtattcc cctcaagaca attacaggca tgggctattt
    43321 attgggagag cgtgagtgag actaatcaaa aaaacctttt tggtcatcaa tggcttaatt
    43381 attgtagtgg ttacctctat tttgttagtg ctttattttg caatgccaat ttattacacc
    43441 aaagtcaaag acaaagaagt taagcgtgaa tttgaccaaa caagcaagca aatcaaaggc
    43501 aaaacggtaa ccgagattag agatatccta acaaaaaaaa tcaacaaaga taatatttgg
    43561 tacagcttag tagacagtga taaccaactg ctctatcctt ccttgcagct gttagacggc
    43621 gtcagtgaaa gcaaagacag tcaaaatgtt aatatcgtca ccacctttga caactcttat
    43681 tctaatgtta aggtgatgag tcaaaaagta accctacgcg atggaaaaaa gatgacattg
    43741 cttgggcaat cttcacttca acccgtaaca gatgctagca aagttctcct tgacctctac
    43801 ccgtcactat tgattttttc agtgaccgta gggagtattg tcgcctactt atatagtagg
    43861 acttctagcc ggcgtatcct tagcatgtca caaaccgcca aaaaaatggt caacctagaa
    43921 ccaaacttaa cgtgtaccat tcacggcaaa gatgaaatag caatgttggc tagtgatatt
    43981 aaccgtttgt atgcgagtct ttcaacgagc atcaaatcct tgcaaaaaga gtatgaaaaa
    44041 gcttctgatt cagaaagaga gaaatctgag tttttacgca tgacatcgca cgagctaaag
    44101 acaccaatca ccagtgttat cggaatgatt gatggaatgc tctataatgt tggtgatttt
    44161 gcagatcgtg acaaatattt gcgaaaatgc cgagacgtcc ttgaagggca agctcaactg
    44221 gttcaatcca ttttatcttt atctaagatt gaaaccctag cttctcaaaa tcaagaactg
    44281 ttttcactaa aaagcagcct agaagaagaa atggaagtct ttctcgtctt atcagaacta
    44341 aaacacctca aagtgactat caatctcgaa gaacaattcg tcaaagccaa taaagtatac
    44401 ctactaaagg cgattaaaaa tattattgac aatgcctttc actataccaa atcaggcggc
    44461 caagtgatga ttcaactaaa agacaaccaa ctagtgatta aaaatgaagc agagacatta
    44521 ttgacacaac agcagatgaa acagttattc caaccgtttt atcgaccaga ttatagtcgt
    44581 aacaggaaag acggtggtac aggattgggg ctgtttatca cccaccagat tcttgatcag
    44641 catcatctgg cttatcgctt tgttgttctt gatcaaagat ggatggtatt tacgattgat
    44701 tttccatccc atcatgacga ttgatccaca ctagtaggac gacaaaagcc atcaatcact
    44761 aaccaagcct tgacctttac ccatcattgt ttaggcaagt ataaaaaaga ggagagaaat
    44821 catatatgaa gaaaaggaaa ttgttagcag taacactatt aagtaccata ctcttaaaca
    44881 gtgcagtgcc attagttgtt gctgacacct ccttgcgtaa taacacatca tccactgatc
    44941 agcctactac agcagatact gatacggatg acgagagtga aacagcaaaa aaagacaaaa
    45001 aaagcaagga aacagcgtcg cagcacgaca cccaaaaaga ccataagtca tcacacactc
    45061 acccaacccc tccttcaaat gatactaagc agaccgatca ggcatcatct gaagctactg
    45121 acaaaccaaa taaagacaaa aacgacacca agcaaccaga cagcagtgat caatccaccc
    45181 catctcccaa agaccagtcg tctcaaaaag agtcacaaaa caaagatggc cgacctaccc
    45241 catcacctga tcagcaaaaa gatcagacac ctgataaaac accagaaaaa tcagctgata
    45301 aaacccctga aaaaggacca gaaaaagcaa ctgaaaaaac cccagagcca aatcgtgacg
    45361 ctccaaaacc catccaacct cctttagcag cagcagctcc tgtctttgca ccgtggagag
    45421 aaagtgacaa agacctgagc aagctaaaac caagcagtcg ctcatcagcg gcttacgtga
    45481 gacactggac aggtgactct gcctacactc acaacctgtt gtcacgccgt tatgggatta
    45541 ctgctgaaca gctagatggt tttttgaaca gtctaggtat tcactatgat aaagaacgct
    45601 taaacgggaa gcgtttatta gaatgggaaa aactaacagg actagacgtt cgagctatcg
    45661 tagctattgc aatggcagaa agctcactag gtactcaggg agtcgctaaa gaaaaaggag
    45721 ccaatatgtt tggttatggc gcctttgact tcaacccaaa caatgccaaa aaatacagcg
    45781 atgaggttgc tattcgtcac atggtagaag acaccatcat tgccaacaaa aaccaaacct
    45841 ttgaaagaca agatctcaaa gcaaaaaaat ggtcactagg ccagttggat accttgattg
    45901 atggtggggt ttactttaca gatacaagtg gcagtgggca aagacgagca gatatcatga
    45961 ccaaactaga ccaatggata gatgatcatg gaagcacacc tgagattcca gaacatctca
    46021 agataacttc cgggacacaa tttagcgaag tgcccgtagg ttataaaaga agtcagccac
    46081 aaaacgtttt gacctacaag tcagagacct acagctttgg ccaatgcact tggtacgcct
    46141 ataatcgtgt caaagagtta ggttatcaag tcgataggta catgggtaac ggtggcgact
    46201 ggcagcgcaa gccaggtttt gtgaccaccc ataaacctaa agtaggctat gtcgtctcat
    46261 ttgcaccagg ccaagcagga gcagatgcaa cctatggtca cgttgctgtt gtagagcaaa
    46321 tcaaagaaga tggttctatc ttaatttcag agtcaaatgt tatgggacta ggcaccattt
    46381 cctatcggac gttcacagct gagcaggcta gtttgttgac ctatgttgta ggggacaaac
    46441 tcccacgacc ataaacccac aaccaagaag ctacctgcct gttgaccaat ctcaaaaaga
    46501 gatagcgtca acttagaatg aggttcatca gatgataaag aaagtgacca ccccgagtca
    46561 aaaaataaaa aaacgtgttc gtaatggcta cctcttaaaa ctaggtacag cctgtttact
    46621 cttaagtata ctcagttatg gcattggtct tttgggccag cctagcatgg aaaatacgtt
    46681 tatgggaata gcgagtgttg ccatgttagg aagtgtctgt ttctttatta tctttgctct
    46741 aaatcgtatc tttgacgcac tagaggacaa tttgcgagat tagagtaata ggtcaaataa
    46801 tcttccaata atttagagca taagctaagt tattggaagt ttttttgtct tttgagagcc
    46861 atgatgccat caaaaagcca aacactcctt atgatgtgag agtcacatga ctaaaattaa
    46921 aagcaaaata gaccccaaag ccctgttttc tcaaaacagc caacgaaaaa agacaagtta
    46981 accagttcat aaaaaacatc gtagaaacat tttcaagagc taatgttggt gtaaattgac
    47041 tgaagtatga tagaattttt aatgaatgtg acaataatgt cacagataaa aaccgaaaac
    47101 agcaaaaata attaaattga atataaaaaa gttaattaag tgagtgataa taaaaaaatg
    47161 aagttcaaaa aatataaata aaataaaaca ccagaactgc taaaagcagt gaatgacata
    47221 aatgtcgcta aaaaaataga tagaagagtc taaaatttca gttttaggtc acgttttatg
    47281 aatagaaatc aggatgatga ccctaagttg tgatttgcgc gtcattgact gctttcctcg
    47341 ttttaatgaa agaatttgaa agatatttta tggtatactt ttccttaata tggttcatac
    47401 ggacttgata aatctattta caaaaataat aaaaaattaa gataaggaca tgaagttaat
    47461 gcatgtaagt aaattattta ctagccaaca atggagagaa ttgaaactga tctcatattt
    47521 aacagaaaac tccaatgcta tcggagttaa agataaggag cttagcaagg ctctcaatat
    47581 ttccatgtta acattacagt catgcctcac taatatgcag tttatgaaag aagtaggtgg
    47641 aattacctac aaagacggct atattaacat ttggtatcac cagtgttgtg ggctacaaga
    47701 ggtctatcaa aaagctcttc gagaatcgcc ttctttgaaa ttactagagc ttttgttttt
    47761 cagagacttt agttccttag aagagttagc tgaagagctg tttgtcagct tatctaccct
    47821 caaacgcctc atcaaaaaaa ccaacactta cttgtctcat acctttgcta ttagtatcgt
    47881 gacaagtcca gtgcaggtat caggagatga gaggcaaatc cgcctgtttt acctcaagta
    47941 tttttcagaa gcctataaaa tctcagaatg gccatttggg gacattttaa acctgaaaga
    48001 ctgtgaacgc ctattgagct tattgatcaa agaagtagat gtcaaagttc attttacact
    48061 atttcagcac ttaaaaatct taagtggtgt caacttgatc cgttactaca aggggtattc
    48121 ttgcagttat aacaataaaa aaaccagtca ccgtttttct caactcatcc aacattcctc
    48181 agaaatccag gatctttctc gcttgttcta cctcaaattt gggttacatc tcgacgagta
    48241 taccatagct gaaatgtttt ctaaccatct taacgacaag ctagaaattg gctgtgcttt
    48301 tgagattatc aatcaagacc caacatcagg aggcagacaa gtaaccaact ggatccatct
    48361 attagatgag atggaaatca agctcaatct cagcatcacc aataaatacg aagttgctgt
    48421 aaccctccat aacgcttcgg tgttaaacga agaagacatt acagccaatt atttgctgtt
    48481 tgattataaa aagagttacc tgaactttta ccaaaaagaa caccctcgca tttatgaggc
    48541 ctttgtgaca agtgtcgaga agctgatgca ggcagataat gctcaagtta gcaaagaatt
    48601 gatcaatcag ctcacttatt gtttctttat tacctgggaa aatagtttct taaaagtaaa
    48661 tcaaagagat gaaaaagtaa gactcctagt gatagagaga agttacaata gtgtcggtaa
    48721 ttttttgaaa aagtacatcg gtgagttttt cagcattact aattttgatg agttagattg
    48781 tttgacaatt gatctagtag agattgaaaa acagtacgat gttatcgtga cagatgttat
    48841 ggtgggtaaa agcgaagagt tagagatttt ctttttctac aagatgatcc cagaagctat
    48901 tattgataga ttaaacgaat ttttgaacgt tagtttcaca gataacaacg ttatggtcaa
    48961 aaacctcgaa gccccttccc cttcaaaatc tcatagtgac aaagaggtac aaaagccaga
    49021 caattcagtt aaacaagcaa catcatcata ggatttcaga cgtcatggta agaaaaaaag
    49081 aatcatttac agcccataga ggtgaggtca aaagctgaaa acagctcaaa aaaactgacc
    49141 tttacctttt ggcttttttt atttagaata attttattgg agagatgctt aataatttaa
    49201 gcacaattct tagaaattaa gaaataagga gtaaacaatg tctaaaagaa atccaaacaa
    49261 gcactactca ctgagaaagt taaaaacagg tactgcatca gtagcagtag ctttgacagt
    49321 tttggggact ggattagcaa acacaactga tgtaaaggct gagaccgtag gtcgctttag
    49381 tgatgaacaa gttagaaagg ctcgtgaaaa agcaatcgaa gacgtgtttg atggctatac
    49441 tggagctcgt tctgtttatc aatctgggaa tctgcctaat aggttaactc ctacaaaact
    49501 tagcaaatta atgcaacaga tgtataagga gactttacaa aagaaagaag aactggatac
    49561 cctatctaaa gctcttacgc acactattga gaaaaagatt gagtcagaaa atgcttacaa
    49621 gaaagagctt ggacagctca aagcagcggc agaagcagaa gctcaaaaag cattagatgc
    49681 actaaataac aaaaacaagc aaatctcaga cttaaccaac gaaaacgcac agttaaaaga
    49741 agccattgaa ggttatgtgc aaactatcca aaacgctagc cgtgaaatcg cagcaaaaca
    49801 acaagaactt gcggctgcaa aaagccagtt agaggcaaaa aatgctgaga ttgaggcatt
    49861 gaaacaacaa gatgcctcta agactgagga aattgctaaa ttgcaatcag aagcagcaac
    49921 tttagaaaac cttctaggtt cagctaagcg tgagttgact gaattgcaag ctaagctaga
    49981 tacagcaact gctgaaaaag caaaactaga atcacaagta acaaccttag aaaaccttct
    50041 aggttcagct aagcgtgaat tgactgatct gcaagctaag ctagatgcag ctaacgctga
    50101 aaaagaaaag ctccaatcac aagcagcagc cctagaaaaa caactagaag caactaaaaa
    50161 agagttagct gatttacagg ctaaattagc agcaaccaac caagaaaaag aaaagttaga
    50221 agctgaagca aaagctctta aggagcaatt agcaaaacaa gctgaagagc ttgctaagct
    50281 aaaagcagat aaagcttcag gagctcaaaa accagatact aaacctggca ataaagaggt
    50341 tccaacaaga ccatcacaaa caagaacaaa cactaataaa gctcctatgg ctcaaacaaa
    50401 gagacaatta ccgtcaacag gcgaagaaac aaccaaccca ttcttcactg cagcagcatt
    50461 gacagtgatc gcatctgcag gcgtacttgc cctaaaacgc aaagaagaaa actaagccca
    50521 acccacacta tcttttctag cccaagaaaa aaacaaaaaa agaggaagac cattcctctt
    50581 tttttgaacg gttaaacagt aaaaaggtca aaaaggtact aaagtctcaa aaacctggtc
    50641 tttacctttt accacttatt ctttagaata gaattattag agagaagtct tagaaaaatg
    50701 aggctaattc cctaaagatg aaaaaataag gagcaaataa tggctagaaa agatacgaat
    50761 aaacagtatt cgcttagaaa attaaaaaca ggtacagcat cagtagcggt cgctgtggct
    50821 gttttaggag caggctttgc aaaccaaaca acagttaagg cggacagtga caatattaat
    50881 agatctgtct ctgtcaaaga taatgaaaaa gaattacata acaaaattgc agaccttgaa
    50941 gaggaaagag gagaacatct agacaaaata gatgaactaa aagaagaact aaaagcaaag
    51001 gagaaaagtt cagaaaacgt agaacgtcac tacctcagaa aactagatca agaatataaa
    51061 gaacaacaag aacgtcaaaa aaatctagag gaactcgaac gccaaagtca acgagaagta
    51121 gaaaaacgtt atcaagaaca actccaaaaa caacaacaat tagaaacaga aaagcaaatc
    51181 tcagaagcta gtcgtaagag cctaagccgt gaccttgaag cgtctcgtgc agctaaaaaa
    51241 gaccttgaag ctgagcacca aaaactcaaa gaggaaaaac aaatctcaga tgcaagccgt
    51301 caaggcctaa gccgtgacct tgaagcgtct cgcgaagcta agaaaaaagt agaagcagac
    51361 ctagctgctc ttactgctga gcaccaaaaa ctcaaagagg aaaaacaaat ctcagacgca
    51421 agccgtcaag gcctaagccg tgaccttgaa gcgtctcgcg aagctaagaa aaaagtagaa
    51481 gcagacttag ccgaagcaaa tagcaaactt caagcccttg aaaaactaaa caaagagctt
    51541 gaagaaggta agaaattatc agaaaaagaa aaagctgagt tacaagcaaa actagaagct
    51601 gaagcaaaag ctcttaaaga gcaattggct aaacaagctg aagaacttgc caaactaaaa
    51661 ggcaaccaaa caccaaacgc taaagtagcc ccacaagcta accgttcaag atcagcaatg
    51721 acgcaacaaa agagaacgtt accgtcaaca ggcgaaacag ctaacccatt ctttacagca
    51781 gcagctgcaa cagtaatggt atctgcaggt atgcttgctc taaaacgcaa agaagaaaac
    51841 taagctatta gactgatgct aaagctaaga gagaatcaaa tgattctctc tttttgagtg
    51901 gctaagtaac taacaatctc agttagacca aaaaatggga atggttcaaa aagctggcct
    51961 ttactccttt tgattaacca tatataataa aaacattagg aaaataatag taatattaag
    52021 tttgtttcct caataaaatc aaggagtaga taatggctag acaacaaacc aagaaaaatt
    52081 attcactacg gaaactaaaa accggtacgg cttcagtagc ggttgctttg accgttttgg
    52141 gcgcaggttt tgcaaaccaa acagaagtaa gagctgaagg ggtaaaagcg actacgaact
    52201 tgccagagga ggctaaatat gccgcattga aagatgagaa tactggttta cgtggtgatc
    52261 agacaaaatt agtaaaaaaa cttgaagaag aacaagagaa gagcaaaaat ctagaaaagc
    52321 aaaaacaaga gttagaaaac caagccctta actttcaaga tgtaattgaa actcaggaaa
    52381 aagaaaaaga agatctcaaa acaactttag ctaaggctac taaagaaaac gagatctcag
    52441 aagctagccg taaagggtta agccgagact tagaagcttc tcgagcagct aaaaaagagc
    52501 tagaagctaa gcatcaaaaa ttagaagcag aaaacaaaaa actaacagaa gccaatcagg
    52561 tttcagaagc tagtcgtaaa ggtctaagta acgacttaga agcttctcga gcagctaaaa
    52621 aagaactaga agctaagtac caaaaattag agactgatca ccaagcccta gaagctaagc
    52681 accaaaaatt agagactgat taccaagttt cagagactag ccgtaagggt ctaagccgtg
    52741 accttgaagc atcacgtgaa gctaataaga aggttacatc tgagttaaca caagcaaaag
    52801 ctcaactcgc agcgcttgaa gaaagtaaga aattatcaga aaaagaaaaa gctgagttac
    52861 aagcaaaact agatgcacaa ggaaaagccc tcaaagaaca attagcaaaa caaactgaag
    52921 agcttgcaaa actaagagct gaaaaagcgg caggttcaaa aacacctgct accaaaccag
    52981 ctaataaaga aagatcaggt agagctgctc aaacagctac aagacctagc caaaataaag
    53041 gaatgagatc acaattaccg tcaacaggcg aagcagctaa cccattcttt acagcagcag
    53101 ctgcaacagt gatggtatct gcaggtatgc ttgctctaaa acgcaaagaa gaaaactaag
    53161 cctttagaac ttggtttttg taacggtgca atagacaaaa gtaagcaagg ccaaaaactg
    53221 agaaagtcct aaaaagctgg cctttacccc tcaaaattaa tgttttataa taaagatgtt
    53281 agtaatataa ttgataaatg agatacattt aatcattatg gcaaaagcaa gaaaaatagc
    53341 tgtatcatat gcaaataacc cctgtttgct ctttaaaaaa gacgttatcc ttatttctcc
    53401 acgcacagat ggacagctag gagagaatcg tttgattctc tcttttctta atggtcataa
    53461 agacaaagtc tctcatcatg aaaggacgac acattgcgta aaaaacaaaa attaccattt
    53521 gataaacttg ccattgcgct catgtctacg agcatcttgc tcaatgcaca atcagacatt
    53581 aaagcaaata ctgtgacaga agacactcct gctaccgaac aagccgtaga aaccccacaa
    53641 ccaacagcgg tttctgagga agcaccatca tcatcaaagg aaactaaccc cccacaaatt
    53701 cctgatgacg cagaagaaac agtagcagat aaggctaatg atccagcccc tcaagctcct
    53761 gctaaaactg ctgatacacc agtaacctca aaagcgacta ttagggattt gaacgaccct
    53821 tctcaggtca aaaccctgca ggaaaaagca ggcaagggag ctgggactgt tgttgcagtg
    53881 attgatgctg gttttgataa aaatcatgaa gcgtggcgtt taacagacaa aaccaaagca
    53941 cgttaccaat caaaagaaga tcttgaaaaa gctaaaaaag agcacggtat tacctatggc
    54001 gagtgggtca atgataaggt tgcttattac cacgactata gcaaagatgg taaaaccgct
    54061 gtcgatcaag agcacggcac acacgtgtca gggatcttgt cagggaatgc tccatctgaa
    54121 acgaaagaac cttaccgcct agaaggtgcg atgcctgagg ctcaattgct tttgatgcgt
    54181 gtcgaaattg taaatggact agcagactat gctcgtaact acgctcaagc tatcagagat
    54241 gctgtcaact tgggagctaa ggtgattaat atgagctttg gtaatgctgc actagcttac
    54301 gccaaccttc cagacgaaac caaaaaagcc tttgactatg ccaaatcaaa aggtgttagc
    54361 attgttacct cagctggtaa tgatagtagt tttgggggca agacccgtct acctctagca
    54421 gatcatcctg attatggggt ggttgggaca cctgcagcgg cagactcaac attgacagtt
    54481 gcttcttaca gcccagataa acagctcact gaaactgcta cggtcaaaac agccgataag
    54541 caagataaag aaatgcctgt tctttcaaca aaccgttttg agccaaacaa ggcttacgac
    54601 tatgcttatg ctaatcgtgg gatgaaagaa gatgatttta aggatgtcaa aggcaaaatt
    54661 gcccttattg aacgtggcga tattgatttc aaagataaga ttgcaaacgc taaaaaagct
    54721 ggtgctgtag gggtcttgat ctatgacaat caggacaagg gcttcccgat tgaattgcca
    54781 aatgttgacc agatgcctgc ggcctttatc agtcgaaaag acggtctctt attaaaagac
    54841 aatcctaaaa aaaccatcac cttcaatgcg acacctaagg tattgccaac agcaagtggc
    54901 accaaactaa gccgcttctc aagctggggt ctgacagctg acggcaatat taagccagat
    54961 attgcagcac ccggccaaga tattttgtca tcagtggcta acaacaagta tgccaaactt
    55021 tctggaacta gtatgtctgc gccattggta gcgggtatca tgggactatt gcaaaagcaa
    55081 tatgagacac agtatcctga tatgacacca tcagagcgtc ttgatttagc taaaaaagta
    55141 ttgatgagct cagcaactgc cttatatgat gaagatgaaa aagcttattt ttctcctcgc
    55201 caacaaggag caggagcagt cgatgctaaa aaagcttcag cagcaacgat gtatgtgaca
    55261 gataaggaca atacctcaag caaggttcac ctgaacaatg tttctgatac atttgaagta
    55321 acagtaacag ttcacaacaa atctgataaa cctcaagagt tgtattacca agcaactgtt
    55381 caaacagata aagtagatgg aaaacacttt gccttggctc ctaaagcatt gtatgagaca
    55441 tcatggcaaa aaatcacaat tccagccaat agcagcaaac aagtcaccgt tccaatcgat
    55501 gctagtcgat ttagcaagga cttgcttgcc caaatgaaaa atggctattt cttagaaggt
    55561 tttgttcgtt tcaaacaaga tcctacaaaa gaagagctta tgagcattcc atatattggt
    55621 ttccgaggtg attttggcaa tctgtcagcc ttagaaaaac caatctatga tagcaaagat
    55681 ggtagcagct actatcatga agcaaatagt gatgccaaag accaattaga tggtgatgga
    55741 ttacagtttt acgctctgaa aaacaacttt acagcactta ccacagagtc taacccatgg
    55801 acgattatta aagctgtcaa agaaggggtt gaaaacatag aggatatcga atcttcagag
    55861 atcacagaaa ccatttttgc aggtactttt gcaaaacaag acgatgatag ccactactat
    55921 atccaccgtc acgctaatgg caaaccatat gctgcgatct ctccaaatgg ggacggtaac
    55981 agagattatg tccaattcca aggtactttc ttgcgtaatg ctaaaaacct tgtggctgaa
    56041 gtcttggaca aagaaggaaa tgttgtttgg acaagtgagg taaccgagca ggttgttaaa
    56101 aactacaaca atgacttggc aagcacactt ggttcaaccc gttttgaaaa aacgcgttgg
    56161 gacggtaaag ataaagacgg caaagttgtt gctaacggaa cctacaccta tcgtgtccgc
    56221 tacactccga ttagctcagg tgcaaaagaa caacacactg attttgatgt gattgtagac
    56281 aatacgacac ctgaagtcgc aacatcggca acattctcaa cagaagatcg tcgtttgaca
    56341 cttgcatcta aaccaaaaac cagccaaccg gtttaccgtg agcgtattgc ttacacttat
    56401 atggatgagg atctgccaac aacagagtat atttctccaa atgaagatgg tacctttact
    56461 cttcctgaag aggctgaaac aatggaaggc gctactgttc cattgaaaat gtcagacttt
    56521 acttatgttg ttgaagatat ggctggtaac atcacttata caccagtgac taagctattg
    56581 gagggccact ctaataagcc agaacaagac ggttcagatc aagcaccaga caaaaaacca
    56641 gaaactaaac cagaacaaga cggttcaggt caagcaccag acaaaaaacc agaagctaaa
    56701 ccagaacaag acggttcagg tcaaacacca gataaaaaac cagaagctaa accagaacaa
    56761 gacggttcag gtcaaacacc agataaaaaa ccagaaacta aaccagaaaa agatagttca
    56821 ggtcaaacac caggtaaaac tcctcaaaaa ggtcaacctt ctcgtactct agagaaacga
    56881 tcttctaagc gtgctttagc tacaaaagca tcaacaagag atcagttacc aacgactaat
    56941 gacaaggata caaatcgttt acatctcctt aagttagtta tgaccacttt cttcttggga
    57001 ttagtagctc atatctttaa aacaaaacgc actgaagatt agtacaactg atcagatggc
    57061 tactattgat gatgagttag atcagtcttc ttttcaaaag aaggcatagc agtataaaaa
    57121 tagatatgtg aggacaatat gcttagagca gaaaataaca aacacagccg ctattctatt
    57181 cgcaagctga gcgttggggt aacgagtata gcaattgcga gtctcttttt aggaaaggtt
    57241 gcctatgccg tagatagcat gcagcaaaac tcttcgactc aaagaaacac agccaataca
    57301 tccaaaaaat ggtatcatat tgatgatagg ggctttattc ctttaggtat aagcttagaa
    57361 gctgccaaaa aagattttaa agaagaagta gaaaaatcac gtttgtctga agaacaaaaa
    57421 caaacgtata atcaaaaaat tgatgctgaa acagacaaag acgaactatt gtcaacgtat
    57481 catagagact atctgacagc cgttaagaat cttccaacgt ctactgagcc agttgaggca
    57541 cccgtgcagg agacacaggc atcagtttca gattcgatgg tgacaggtga ttcaacatca
    57601 gttacgactg attctcctga ggaaacacta tctacagaaa ccccatctcc atcacctgag
    57661 agtgagaaat cttcagtagc agcttcttct gaggaaacct catctccaga aactccatca
    57721 actccagagg ccccagtagc cccagcttta tctgaggctc catctccatc acctgagagt
    57781 gaggaacctt cagtagcagc ttcttctgag gaaaccccat ctccatcaac tccagcagcc
    57841 ccagaaactc cagcggctcc agaaactcca gcggctccag aaactcctga ggaaccttca
    57901 gtagcagctc cttctgagga aaccccatct ccatcaactc cagcagcccc agaaatccca
    57961 gctgaatcag aggctccagc tcaaccagct gagagtgaag aatcttcagt agcagctacg
    58021 acaagtccgt ctccatcaac tccagctgaa tcagagactc agacgccatc agctgttact
    58081 aaagactctg ataagccatc ttcagcagct gaaaaaccag cagcctcttc acttgtttca
    58141 gaacaaaccg ttcaacaacc aacttcaaag agatcttctg ataaaaaaga agagcaagaa
    58201 cagtcttact ctccaaatcg ctcattgtca agacaggtta tggcccatga gtcaggtaag
    58261 tacttgcctt caacaggtga aaaagcacag ccactcttta tagctactat gactttgatg
    58321 tctctatttg gcagtctttt agtcacaaaa cgccaaaaag aaactaaaaa atagtattac
    58381 ttaaactgat aagagcttat ttaatagaga gaaccaattt ggttctctct atttttttag
    58441 atgtgcagcc tcatcaaaaa gaagttagga caaatagctg tcaacaattg ccttttgctt
    58501 atcccttcag ttgctgtgat tttatcatac agtgataaaa gatggtagtt gccattgaca
    58561 aagcattgat aaaggagtaa aattaactgg ttaataactg gttaaattat aattgaggag
    58621 gtactatgaa aaaaggtttt tttctcatgg ctatggctgt gagtttagta atgatagcag
    58681 ggtgtgataa gtcagcaaac cccaaacagc ctacgcaagg catgtcagtt gtaaccagct
    58741 tttacccaat gtatgcgatg acaaaagaag tatctggaga cctcaatgat gtgaggatga
    58801 tccaatcagg tgcaggcatt cattcctttg aaccgtctgt aaatgatgtg gcagctattt
    58861 atgacgcgga tttgtttgtt taccattcac ataccttaga agcttgggca agggatctag
    58921 accctaattt aaaaaaatca aaggttgatg tgtttgaagc gtcaaaacct ctgacactag
    58981 atagagtcaa agggctagaa gatatggaag tcacacaagg cattgaccct gcgacacttt
    59041 atgacccaca tacctggaca gatcccgttt tagctggtga ggaagctgtc aatatcgcta
    59101 aagagctagg acgtttggat cctaaacaca aagacagtta cactaaaaag gctaaggctt
    59161 tcaaaaaaga agcagagcaa ctaactgaag aatacactca aaaatttaaa aaggtgcgct
    59221 caaaaacatt tgtgacgcaa cacacggcat tttcttatct ggctaaacga ttcggcttga
    59281 aacaacttgg tatctcgggt atttctccag agcaagagcc ctctcctcgc caattgaaag
    59341 aaattcaaga ctttgtcaaa gaatacaacg tcaagactat ttttgcagaa gacaatgtca
    59401 atcccaaaat tgctcatgct attgcgaaat caacaggagc taaagtaaag acattaagtc
    59461 cacttgaagc tgctccaagc ggaaacaaga catatctaga aaatcttaga gcaaatttgg
    59521 aagtgctcta tcaacagttg aagtaaagga gattattagt gaagaaaaca tatggttata
    59581 tcggctcagt tgctgccatt ttactagcta ctcatattgg aagttaccaa cttggtaagc
    59641 atcatatggg tccagcaaca aaggacaatc agattgccta tattgatgat agcaaaggta
    59701 aggcaaaagc ccctaaaaca aacaaaacga tggatcaaat cagtgctgaa gaaggcatct
    59761 ctgctgaaca gatcgtagtc aaaattactg accaaggtta tgtgacctca cacggtgacc
    59821 attatcattt ttacaatggg aaagttcctt atgatgcgat tattagtgaa gagttgttga
    59881 tgacggatcc taattaccgt tttaaacaat cagacgttat caatgaaatc ttagacggtt
    59941 acgttattaa agtcaatggc aactattatg tttacctcaa gccaggtagc aagcgcaaaa
    60001 acattcgaac caaacaacaa attgctgagc aagtagccaa aggaactaaa gaagctaaag
    60061 aaaaaggttt agctcaagtg gcccatctca gtaaagaaga agttgcggca gtcaatgaag
    60121 caaaaagaca aggacgctat actacagacg atggctatat ttttagtccg acagatatca
    60181 ttgatgattt aggagatgct tatttagtac ctcatggtaa tcactatcat tatattccta
    60241 aaaaagattt gtctccaagt gagctagctg ctgcacaagc ctactggagt caaaaacaag
    60301 gacgaggtgc tagaccgtct gattaccgcc cgacaccagc cccaggtcgt aggaaagccc
    60361 caattcctga tgtgacgcct aaccctggac aaggtcatca gccagataac ggtggctatc
    60421 atccagcgcc tcctaggcca aatgatgcgt cacaaaacaa acaccaaaga gatgagttta
    60481 aaggaaaaac ctttaaggaa cttttagatc aactacaccg tcttgatttg aaataccgtc
    60541 atgtggaaga agatgggttg atttttgaac cgactcaagt gatcaaatca aacgcttttg
    60601 ggtatgtggt gcctcatgga gatcattatc atattatccc aagaagtcag ttatcacctc
    60661 ttgaaatgga attagcagat cgatacttag ccggccaaac tgaggacaat gactcaggtt
    60721 cagatcactc aaaaccatca gataaagaag tgacacatac ctttcttggt catcgcatca
    60781 aagcttacgg aaaaggctta gatggtaaac catatgatac gagtgatgct tatgttttta
    60841 gtaaagaatc cattcattca gtagataaat caggagttac agctaaacac ggagatcatt
    60901 tccactatat aggatttgga gaacttgaac aatatgagtt ggatgaggtc gctaactggg
    60961 tgaaagcaaa aggccaagct gatgagcttg ctgctgcttt ggatcaggaa caaggcaaag
    61021 aaaaaccact ctttgacact aaaaaagtga gtcgcaaagt aacaaaagat ggtaaagtgg
    61081 gctatatgat gtcaaaagat ggcaaggact atttctatgc tcgtgatcaa cttgatttga
    61141 ctcagattgc ctttgccgaa caagaactaa tgcttaaaga taagaagcat taccgttatg
    61201 acattgttga cacaggtatt gagccacgac ttgctgtaga tgtgtcaagt ctgccgatgc
    61261 atgctggtaa tgctacttac gatactggaa gttcgtttgt tatccctcat attgatcata
    61321 tccatgtcgt tccgtattca tggttgacgc gtgatcagat tgcaacaatc aagtatgtga
    61381 tgcaacaccc cgaagttcgt ccggatgtat ggtctaagcc agggcatgaa gagtcaggtt
    61441 cggtcattcc aaatgttacg cctcttgata aacgtgctgg tatgccaaat tggcaaatta
    61501 tccattctgc tgaagaagtt caaaaagccc tagcagaagg tcgttttgca acaccagacg
    61561 gctatatttt cgatccacga gatgttttgg ccaaagaaac ttttgtatgg aaagatggct
    61621 cctttagcat cccaagagca gatggcagtt cattgagaac cattaataaa tctgatctat
    61681 cccaagctga gtggcaacaa gctcaagagt tattggcaaa gaaaaacgct ggtgatgcta
    61741 ctgatacgga taaacccaaa gaaaagcaac aggcagataa gagcaatgaa aaccaacagc
    61801 caagtgaagc cagtaaagaa gaagaaaaag aatcagatga ctttatagac agtttaccag
    61861 actatggtct agatagagca accctagaag atcatatcaa tcaattagca caaaaagcta
    61921 atatcgatcc taagtatctc attttccaac cagaaggtgt ccaattttat aataaaaatg
    61981 gtgaattggt aacttatgat atcaagacac ttcaacaaat aaacccttaa ccaaaagaag
    62041 atctcattat taaagcactg ctttatcaaa gcaagttacg gtgattttga agtcatttta
    62101 tgtaacgagt agtgataaaa gttggataat agcggttttc ttttgcaaag aaatggtatc
    62161 catgttagaa taataaaaaa aagaggagga ttctaatcat gtcagaagaa aaattaaaat
    62221 caaaaattga gcaagcatct ggtggcctaa aagaaggtgc tggtaagttg acaggtgata
    62281 aagagctaga agcaaaagga tttgttgaaa aaacaattgc taaaggcaaa gaacttgcag
    62341 acgatgcaaa agaagcggtt gaaggtgctg tagatgccgt taaggaaaaa ttaaaataat
    62401 taaaaaacca gctgtaagct ggttttttaa tggtgtgatt ttgttagaaa aagactcaat
    62461 aggtaataac catagatgtt aggtatgctt gaacagttga gtacgattct cttcaatcat
    62521 atagcacgcg tcacaaagtt tttctaacat atcaaaatca tgactaataa tgatgagacc
    62581 taaatttcta tcctttacaa tctccaacag gcttttccat acactagctt gtgtaatgct
    62641 atccaacata gtagtcattt catctgcaat aaggtatttc gtctctggat gtaatgaacg
    62701 cacaatcgaa aagcgttgca attcccctcc cgagagttca ctagggcgac gctttagcca
    62761 tttttcttga atgccaaaag catctcgcaa atcttgactt ggatagtagg cttcttccaa
    62821 actttttttc ataggccata aaggattcat ggtttgttca ggatgttgtt ggataagttg
    62881 cacaggtcta aatgctttgt taggcaaatg actgccatca actaatactt caccagattt
    62941 aggctgcaaa aaaccggcaa gcactctgga taaactcgtt tttccacacc cactttgtcc
    63001 aaatatccct aagatttgac caggtgctac ctctaaatca atctccttaa aaagccattg
    63061 atctttttta tgataaaagc caagcttttt agcctctaag gtcatgagta actcctttca
    63121 aaaagtcttg ctgagggagt gtgcgccata aacgtctcgc aaattctgtt tgtaattgct
    63181 ccccacctcc gctaaaaaag ctagctgggg ctgtttcaat agcttttccc tctttaaaaa
    63241 tagttatacg atccgcaatt tgactagctg ctacaatatc atgagtgata aagatgacgc
    63301 ttattccttt atctgcaaaa gagcgtagtt gatctaaaac catttgcaga gcatctggat
    63361 gtaatccagg ggtgggctca tccgcaataa tcaaagaaac cgtatcacta atacacgttg
    63421 taaacaaaac acgtcggagc attccgccag aaagttggaa aggatacaag tcaccatcac
    63481 tttcttttaa accaaactgt tgaaataatc cttcttgagt agccttagca ttttctgaga
    63541 tacccaagcg cacctgatgc ttgactttca tagatggatc taaataatta acggattgtg
    63601 gaatcaacgt catttctttt cctcgcaact gtttaatgcg tttagaagtt agtgattgac
    63661 cacgataaat catatctcct gttacagctg catttttagg aagaatatcc ataatagcgt
    63721 gtgctaataa actcttccct gaaccactag cacctataat agctaacaac tcaccttttt
    63781 taacttctaa attcagcgct tgtatcggtg tggattgaaa tggttttaaa aatcttccgt
    63841 attgagtgaa ggtgatggag aggtctttaa tgcttaataa tgtttctgtc atatctctac
    63901 ctcctaaaaa tgatccgttt gagggtaaaa gagtttcttt aaagattctc cgatagtatc
    63961 aaatgcattg acaaccaaaa taagataaag gcctggaaaa atcaccaacc accaattgcc
    64021 aagagagata tgcttagctg cctctgataa aatgatacca accgaaggtt gttcggcaga
    64081 aaggccaaat cctaagaaag tcatggatgc ttcatgcaag atgacgtgcg gaaataagag
    64141 gataaaccca atgaaaattt gagaagcaat caaaggcaag atatgatgcc tcacaatata
    64201 ataaggcgtt tttcccatgc ttttagagag ctggacaaag gctttattct ttagatcata
    64261 gacttcattg cggataagcc ttgctagaga gggccaatgg gtaacagctg ttgcaatgat
    64321 aaccccttga gcccctttcc caacaacaaa agaaatgaga atcataaaaa tcaaatgagg
    64381 cataccaata aacaaatcaa ccaaccaggc tattatttta tcaataaggc tatttcctag
    64441 acctgcaagc actccaaaaa cggtcgcaag aaagactccc ataagggcac ctaataagcc
    64501 gacttgtaaa gagaaataaa gccctttaat cgttctgaca aacatatccc tacctaaacc
    64561 atctgtccca aaaagatggt ttaaggaagg agcaaggttg cgtaaagctg cattggtttc
    64621 caaaggcgtc ctatagaaat aaaggtttaa ggctagaata ctaagaatga gagaaatggc
    64681 gatacccagt tgccataaaa ccatcgttcg acgtttcaat atcatacttt tctccttaac
    64741 tgtggattga tgatgctatt taaaatgtcc gcaataaaat tgcccgcaaa aacaaataat
    64801 gtccctatca tcacaatagc tagaagtagc ggtgtatcac ttttaaggcc tgcttcagtg
    64861 agggtagacc ctaagccagg atatgagaaa acttgctcag caagaacaga tcctccaaac
    64921 aactctccaa aataggaaaa atgcagtgta atagctggta cgatagcatt tctaagacaa
    64981 tgatgtttaa aaatttgcca ctgtgtttcc ccacgcgctc tggcaaataa gacatattca
    65041 ctagaaagca ccgacatcat tttagttctc gtatgaagag tgacattggc gatgcctaga
    65101 atacttagcg tgaaaacagg taacataagg tgcttaatcc gatcagctaa cgtaatatct
    65161 tgactcaaag tgcctatcgg agaagaaata ccaatcggga accaccccag ctggacagaa
    65221 aagattaata aaaaaatgag tccaatccaa aacgttggta ctgatatctg aaggtatgaa
    65281 aaccacctga taactcggtc aagtaatttc ccttgatgga aagcggataa cgttcctaag
    65341 ataaatccaa taagacccga taagatccaa gagagtccca taagtatgaa agaagcacct
    65401 gcccgtgatc taataatatc actaacaggt tgccgataaa cgagcgaggt ccctaaatgt
    65461 ccctgtatca catttttcaa ccaaataaaa tactggacta gagctggctt atccaagcca
    65521 tagtggtgag caatcgcttt gtactgagca ggggttagtg atgtgtcata attgacactt
    65581 gccatgactg gatctactgg agattgtttt aacaaaacga aggtcaaaac agatacccca
    65641 aaaataagcg tgacacatct gatgattttc cagatgataa taatggtagt acgtttcaca
    65701 atgaaatctc cttaatcgat aagaaaagga gccgaggaaa cgatcagcac agttttgtgt
    65761 atcttgtttt ctcagactcc aaacttaaag tcgctgtatt aactaaacgc gttacttagt
    65821 tgattcatcc caagtccact cggcaatgtt agtcaataat gaccaatcat gaccatgact
    65881 gtggatgcct tgtttaccta cattgatacg tttatcacca atataagtat ggttaaggct
    65941 caccaaccat acatttggca aatctccaag agtagaagca cctgttttgc catcccactg
    66001 cgctaacttc caatattcgt tagctttgtc aaggtcagaa gatgtcattg ctttgtcaag
    66061 gtacttagtc acggtaggat tgttataaaa cgtaatattg gtccaacctt tacctgctag
    66121 gcttggatga tgtgattcat aaaattgctg cgcgtgatga cgtcctccgg cataaagtaa
    66181 ggctgagtca tgtgacttcg ttgccatttc atcccagtta ctagctttga gtttaatagt
    66241 aatccctagg gctttggctt gctctgctac ttcaacggct aagttcgctc gcaattgatc
    66301 attagtaggg tagtacagat caaacgctgc atcaaggtca ccttttttac ggctaccgtc
    66361 tgcttgttct ttccatcccg cttttgtcaa caattgctta gctttagcta ctttattatc
    66421 tttaatggct gtttttggat tccaaaatgg tgttttatca ataattgaat aagctggttt
    66481 accataacca tttaaaaccg tatcgagaac tttttgccta tttaaaccaa tagtcaaggc
    66541 tttgcggatt gctggatcac tagtgacatc atttcctaca ggataaccat caggagaatc
    66601 agtgatgacg ccttttttca cataaggtaa tgataagccg cgcacatcat ttgatggaat
    66661 atcaaggagg cgggtgcctt tgactttttt atcagcaagt tctggtgtcg cgtagatcat
    66721 atcaacatca ccagattcta aagctgctag tgctgtgttt tcatcaagta agacccaagt
    66781 ccacttttta aagtatggtt ttttcccatg ccagtaaggg ttacgaacaa aaatagcttg
    66841 ttctccagcc ttatattctt ttaccatgta aggtcctgaa ccgataggat tgctcttata
    66901 cttatcattg taatgttttt tagggacgat tgggatttca gtcaactgtg ctgtaaatgt
    66961 cgaatgcgcc tcagtcaaat ggatattgac ctgatttttc ccaactactt caacgttctt
    67021 gatgaaggtt agatcccaag cctttccatc tgctttcaac atatcataag taaacttaac
    67081 atcatcagca gtaacaggct caccatttga gaatttaaaa tcatcatgca agtcaaacga
    67141 ccaagtcagc ccatcttcag agagatggta tgttttagca agctctcctt ttatatctag
    67201 ttcaggagaa cgtttcaata gagtgctatg agtgatattc ccttcattgt ggactccata
    67261 acggtccttt ggatcgaatt catgagggag ctttgccccc atagaaacga caagttcgtc
    67321 ttttggacgt tgtttgcgct gacgttcttt tgtttgaggc ttttgttgtt gacatgcaac
    67381 taaaataagc ccagtcaaaa ataacgtgat aatagagaag tattttaggt attttgacac
    67441 aatcaaatct ccttatttct aattaataaa taataatagt aacgactcca tctcattata
    67501 gcaataaaca aataggaggg caattgttta actggttaat tagaattttt gttattttca
    67561 ggaa
//
LOCUS       DAUGFP010000006        62533 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.006, whole genome shotgun sequence.
ACCESSION   DAUGFP010000006 DAUGFP010000000
VERSION     DAUGFP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 62533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62533
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.006"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..843
                     /locus_tag="VH663_000354"
     CDS             <1..843
                     /locus_tag="VH663_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEQ8173583.1"
                     /translation="KVVSVISLTLSLFLVTGCAKVDNNKSVNRKPATKQTYNSYSDDQ
                     LRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDETVLD
                     NSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISDRSTT
                     QVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLLDFAD
                     FSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEERRKSL
                     KSFK"
     gene            1036..2232
                     /locus_tag="VH663_000355"
     CDS             1036..2232
                     /locus_tag="VH663_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEQ8173584.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAEFDQALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            2495..3046
                     /locus_tag="VH663_000356"
     CDS             2495..3046
                     /locus_tag="VH663_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ8173585.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKSGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWFNKKHSDVR"
     gene            3113..3484
                     /locus_tag="VH663_000357"
     CDS             3113..3484
                     /locus_tag="VH663_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ8173586.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            3522..4868
                     /gene="glnA"
                     /locus_tag="VH663_000358"
     CDS             3522..4868
                     /gene="glnA"
                     /locus_tag="VH663_000358"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEQ8173587.1"
                     /translation="MAITVADIRHEVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDETDKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPIEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFISQWEIDHYIHNY"
     gene            complement(5096..6778)
                     /locus_tag="VH663_000359"
     CDS             complement(5096..6778)
                     /locus_tag="VH663_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ8173588.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(6780..7010)
                     /locus_tag="VH663_000360"
     CDS             complement(6780..7010)
                     /locus_tag="VH663_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEQ8173589.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            7293..7991
                     /gene="tsaB"
                     /locus_tag="VH663_000361"
     CDS             7293..7991
                     /gene="tsaB"
                     /locus_tag="VH663_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEQ8173590.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHAPLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            7963..8418
                     /gene="rimI"
                     /locus_tag="VH663_000362"
     CDS             7963..8418
                     /gene="rimI"
                     /locus_tag="VH663_000362"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEQ8173591.1"
                     /translation="MIVTTLSESNMKTVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIW
                     RDQTDYFLLYDHDKLLGFLAIQDLAGEVEMTQIAILPSHQGLGLASQLMTHLGSIESD
                     IFLEVRESNHRAQGLYQKFGFKFIGKRPDYYRNPIETALLMKREGKNDR"
     gene            8408..9436
                     /gene="tsaD"
                     /locus_tag="VH663_000363"
     CDS             8408..9436
                     /gene="tsaD"
                     /locus_tag="VH663_000363"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEQ8173592.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGDELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(9607..9876)
                     /gene="rpsN"
                     /locus_tag="VH663_000364"
     CDS             complement(9607..9876)
                     /gene="rpsN"
                     /locus_tag="VH663_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEQ8173593.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(10097..10855)
                     /locus_tag="VH663_000365"
     CDS             complement(10097..10855)
                     /locus_tag="VH663_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8173594.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSCQSDTH"
     gene            11096..11875
                     /gene="udp"
                     /locus_tag="VH663_000366"
     CDS             11096..11875
                     /gene="udp"
                     /locus_tag="VH663_000366"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEQ8173595.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            11896..13098
                     /locus_tag="VH663_000367"
     CDS             11896..13098
                     /locus_tag="VH663_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEQ8173596.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            13128..13799
                     /gene="deoC"
                     /locus_tag="VH663_000368"
     CDS             13128..13799
                     /gene="deoC"
                     /locus_tag="VH663_000368"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEQ8173597.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECEDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAKCQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAETFIALGASRLGTSRIIKIVKNEAT
                     KPDSY"
     gene            complement(13894..15063)
                     /locus_tag="VH663_000369"
     CDS             complement(13894..15063)
                     /locus_tag="VH663_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ8173598.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDDLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVRLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGKVLTSTEVVSVKKQDDLFYLKSADQTFSCHKLVV
                     TTGGKSYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
     gene            15268..15798
                     /locus_tag="VH663_000370"
     CDS             15268..15798
                     /locus_tag="VH663_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEQ8173599.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFLTRLFAK"
     gene            15847..16434
                     /locus_tag="VH663_000371"
     CDS             15847..16434
                     /locus_tag="VH663_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEQ8173600.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHIIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            16586..17323
                     /locus_tag="VH663_000372"
     CDS             16586..17323
                     /locus_tag="VH663_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ8173601.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTAGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKLQWKMGISIFLAIVSYVGLILL
                     SNYLQKFWLVYVAMGLFLPGFSWLVIYFRKQFIYLCPHCHQKFEPSFNEFALAGHTPR
                     TRKLTCSHCHQKSYCLELAKEDTVSRG"
     gene            complement(17380..17679)
                     /locus_tag="VH663_000373"
     CDS             complement(17380..17679)
                     /locus_tag="VH663_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEQ8173602.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDEATKKTLGAFAGKLPF"
     gene            complement(17872..18246)
                     /locus_tag="VH663_000374"
     CDS             complement(17872..18246)
                     /locus_tag="VH663_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8173603.1"
                     /translation="MSAKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKG
                     KYTPKSGLLSDIAAIFDVSLTALMEEDLTLESKGVSYQLPQRELAMISEQLSENNYTK
                     WVDFAQILLEQQAKEDRELDNH"
     gene            18416..18637
                     /locus_tag="VH663_000375"
     CDS             18416..18637
                     /locus_tag="VH663_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8173604.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            complement(18717..20999)
                     /locus_tag="VH663_000376"
     CDS             complement(18717..20999)
                     /locus_tag="VH663_000376"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEQ8173605.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKL
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKANIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDRYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYTHGLQDWNVKPRQVYEIVNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFETSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRAFKAALFKGKANQALVDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGNTLRVILYTTDFEHTVRDNS
                     NYTLTIDLSQSQLIVPIASN"
     gene            complement(21021..21680)
                     /locus_tag="VH663_000377"
     CDS             complement(21021..21680)
                     /locus_tag="VH663_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ8173606.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            21846..23042
                     /locus_tag="VH663_000378"
     CDS             21846..23042
                     /locus_tag="VH663_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8173607.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPARKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMIIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTFLYLLKTK
                     TRQVVS"
     gene            23514..24362
                     /locus_tag="VH663_000379"
     CDS             23514..24362
                     /locus_tag="VH663_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ8173608.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKVSYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(24698..25594)
                     /locus_tag="VH663_000380"
     CDS             complement(24698..25594)
                     /locus_tag="VH663_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEQ8173609.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYFGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            25892..26644
                     /locus_tag="VH663_000381"
     CDS             25892..26644
                     /locus_tag="VH663_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEQ8173610.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDALFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            26629..27579
                     /locus_tag="VH663_000382"
     CDS             26629..27579
                     /locus_tag="VH663_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEQ8173611.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKVYYPSIADTTVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKVLNHLTQKENKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTIERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
     gene            complement(27759..30086)
                     /gene="pflB"
                     /locus_tag="VH663_000383"
     CDS             complement(27759..30086)
                     /gene="pflB"
                     /locus_tag="VH663_000383"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEQ8173612.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            30295..31389
                     /gene="dinB"
                     /locus_tag="VH663_000384"
     CDS             30295..31389
                     /gene="dinB"
                     /locus_tag="VH663_000384"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEQ8173613.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDLVEPMSIDEAYLDVTDNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKVRGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGVTMTNLEDKVADISLDL"
     gene            complement(31482..31964)
                     /locus_tag="VH663_000385"
     CDS             complement(31482..31964)
                     /locus_tag="VH663_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173614.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSVLNVLAIIAIIVFCAQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTLSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(32055..34508)
                     /locus_tag="VH663_000386"
     CDS             complement(32055..34508)
                     /locus_tag="VH663_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEQ8173615.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKIADALAENLGIESDSPKRFR
                     AALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTYFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQAVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QPNQELKSKEQTSLFSNTATLEDDSQKSSSQSTNSNPTENSQSDNDDFRLTPENYSTI
                     DSMIGLTESDIALFFQKKS"
     gene            complement(34566..35159)
                     /gene="lepB"
                     /locus_tag="VH663_000387"
     CDS             complement(34566..35159)
                     /gene="lepB"
                     /locus_tag="VH663_000387"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ8173616.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            complement(35170..36072)
                     /locus_tag="VH663_000388"
     CDS             complement(35170..36072)
                     /locus_tag="VH663_000388"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEQ8173617.1"
                     /translation="MNTLVLKIDAILSKHLKKQLAPYTISSQNTYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHPFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNAISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            36229..36537
                     /locus_tag="VH663_000389"
     CDS             36229..36537
                     /locus_tag="VH663_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173618.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            36540..37085
                     /locus_tag="VH663_000390"
     CDS             36540..37085
                     /locus_tag="VH663_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEQ8173619.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            37234..39573
                     /locus_tag="VH663_000391"
     CDS             37234..39573
                     /locus_tag="VH663_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEQ8173620.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNISFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISNDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPILADKGSKIAVFNNIFADIGDEQSIEQNLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFSLVRVQEEQ
                     KVKNKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRHKHVKHFAYAPQNAGGSGATIVTLG"
     gene            39577..40077
                     /locus_tag="VH663_000392"
     CDS             39577..40077
                     /locus_tag="VH663_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ8173621.1"
                     /translation="MESYEHFYAKLSQPFRKTPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            40158..40472
                     /gene="trxA"
                     /locus_tag="VH663_000393"
     CDS             40158..40472
                     /gene="trxA"
                     /locus_tag="VH663_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEQ8173622.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQTPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(40524..41111)
                     /locus_tag="VH663_000394"
     CDS             complement(40524..41111)
                     /locus_tag="VH663_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8173623.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSHQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFKRDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQPYKEILAVSSGKLLDEIEKREE
                     RAKLPYQNPLIVTVFFLITVAIFFASRFMFTWLFH"
     gene            complement(41288..42412)
                     /gene="mutY"
                     /locus_tag="VH663_000395"
     CDS             complement(41288..42412)
                     /gene="mutY"
                     /locus_tag="VH663_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEQ8173624.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKHFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQSGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            42610..42903
                     /locus_tag="VH663_000396"
     CDS             42610..42903
                     /locus_tag="VH663_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173625.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            43076..43366
                     /locus_tag="VH663_000397"
     CDS             43076..43366
                     /locus_tag="VH663_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEQ8173626.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGANVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            43388..43879
                     /locus_tag="VH663_000398"
     CDS             43388..43879
                     /locus_tag="VH663_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ8173627.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            44044..44283
                     /locus_tag="VH663_000399"
     CDS             44044..44283
                     /locus_tag="VH663_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEQ8173628.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(44416..45072)
                     /locus_tag="VH663_000400"
     CDS             complement(44416..45072)
                     /locus_tag="VH663_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEQ8173629.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQRAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGVSLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(45204..46148)
                     /locus_tag="VH663_000401"
     CDS             complement(45204..46148)
                     /locus_tag="VH663_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEQ8173630.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            46351..49179
                     /gene="uvrA"
                     /locus_tag="VH663_000402"
     CDS             46351..49179
                     /gene="uvrA"
                     /locus_tag="VH663_000402"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEQ8173631.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPIVRRKK
                     GQHKTIFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLKEGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            49294..50367
                     /locus_tag="VH663_000403"
     CDS             49294..50367
                     /locus_tag="VH663_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ8173632.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLNVVAELLGADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRLIKDVSEIETIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIAASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIAKASAGMTYSDFDGIPRQL
                     ITEAGYGSHFTHGIGHGIGLDIHENPFLGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITKTGCQVLTLAPKELIVL"
     gene            50402..50863
                     /locus_tag="VH663_000404"
     CDS             50402..50863
                     /locus_tag="VH663_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEQ8173633.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGITKITYKAHYRPHPFAIELMEKKGVAYVQHDVPQIVLGGDKEQ"
     gene            50959..51516
                     /gene="efp"
                     /locus_tag="VH663_000405"
     CDS             50959..51516
                     /gene="efp"
                     /locus_tag="VH663_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEQ8173634.1"
                     /translation="MIEASKLKAGMTFEAEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            51562..51951
                     /locus_tag="VH663_000406"
     CDS             51562..51951
                     /locus_tag="VH663_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ8173635.1"
                     /translation="MTTEYIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            51944..52396
                     /gene="nusB"
                     /locus_tag="VH663_000407"
     CDS             51944..52396
                     /gene="nusB"
                     /locus_tag="VH663_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEQ8173636.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(52537..53502)
                     /locus_tag="VH663_000408"
     CDS             complement(52537..53502)
                     /locus_tag="VH663_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ8173637.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEILKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(53507..54946)
                     /locus_tag="VH663_000409"
     CDS             complement(53507..54946)
                     /locus_tag="VH663_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEQ8173638.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGRYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDLDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGRA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTMRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVEGQAFGHYYEMRY"
     gene            55188..57071
                     /locus_tag="VH663_000410"
     CDS             55188..57071
                     /locus_tag="VH663_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ8173639.1"
                     /translation="MDNRQIAAEVIEALGGRENVRSVAHCATRLRVMVYDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSILGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSTKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGQLLQYLVTMLVGLGVAFAIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSTDYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            57302..60274
                     /locus_tag="VH663_000411"
     CDS             57302..60274
                     /locus_tag="VH663_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEQ8173640.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEEKTVQVQ
                     KELSSIDSLHYLSENSKKEFKEELSKEKVPEKVKEILAKAQQADKQAQELTKMKIPEK
                     IPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLFW
                     KELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIVD
                     EYVYKYNLDGLDVDVEHDSIPKVNGKASDENLKRSIDVFEEIGKLIGPKGVDKSRLFI
                     MDSTYMADKNPLIERGAPYIDLLLVQVYGARGEQGEFQNDTKLVTETPEERWQGYSKY
                     IRPEQYMIGFSFYEERAGSGNLWYDINSRKDDDKANGINTDITGTRAERYARWQPKTG
                     GVKGGIFSYAIDRDGVAHQPKQIAEKDKQNVKNNQPQIPEITDNIFHSDYSVSKALKT
                     VMLKDKSYDLIDEKDFPDKALREAVMAQVGTRKGDLERFNGTLRLDNPAIQSLEGLNK
                     FKKLAQLDLIGLSRITKLDQSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSLK
                     VSGLTGLKELDLSGFDRETLAGLDAATLTSLEKVDISGNKLDLAPGTENRQIFDTMLS
                     TISNHVGSNEQTVKFDKQKPTGHYPDTYGKTSLRLPVAEGNIDLQSQLLFGTVTNQGT
                     LINSEADYKAYQNHKIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLATD
                     KEETYKVDFFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADSQAALKAFDG
                     VIEYNPISTQNRASIIFEMKDPSLAKYWRLFNDSSKGEDDYIKEAKLEVFTGQLNAEA
                     DVKTSLEKSDDWQTVSTYSGQEQVFSHALDNISAKYWRVTVDTKGGNYSWPSLPELQI
                     LGYPLPNADTIMKTVTTAKELSQQKDKFSQKILDELKIKEMALETSLNSKIFDVTAID
                     ANAGVLKDCIEKRQLLKK"
     gene            60538..61422
                     /locus_tag="VH663_000412"
     CDS             60538..61422
                     /locus_tag="VH663_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ8173641.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIAQT
                     IAYFKTFEADLAGMAIGSFGPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     LHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMAQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            61517..62470
                     /gene="manA"
                     /locus_tag="VH663_000413"
     CDS             61517..62470
                     /gene="manA"
                     /locus_tag="VH663_000413"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEQ8173642.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATMVLDNMVATTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
BASE COUNT        19097 a        10932 c        13099 g        19405 t
ORIGIN      
        1 aaagttgtta gtgttatatc acttacctta tccctttttt tggtgacagg ttgtgctaaa
       61 gttgataaca acaaatcagt taatcgtaag cctgctacta aacaaaccta taatagctat
      121 agtgatgacc aattaagatc gcgtgaaaat accatgtctg ttttatggta ccagcgagct
      181 gcggaaactc aagcgcttta tttacagggg tatcagttag caacggatcg cttaaaagaa
      241 caactcaata aaccaacgga taaaccttat tcaattgtat tagatattga cgaaacagtt
      301 cttgataata gcccttatca agctaaaaat gttttggaag gaacaggatt tacacctgaa
      361 agctgggatt attgggtaca aaagaaagaa gcaaaaccgg ttgctggtgc taaagacttt
      421 ttgcaatttg cagaccaaaa tggtgttcaa atttactaca tttcagacag atcaactact
      481 caagtagatg ctacaatgga aaatctccaa aaagaaggta ttccagtaca aggtcgtgat
      541 catcttctat tcttagaaaa aggcgtaaaa tcaaaggaga gtcgtcgtca aaaggtcaaa
      601 gaaacaacta atgtaacgat gctatttggt gataatcttc tagattttgc tgatttttca
      661 aaaaaatctc aagaagatag aacagcttta ttatcagatt tgcaagaaga gtttggaaga
      721 cgctttatca ttttccctaa tcctatgtat ggttcatggg agggtgccat ttataaaggt
      781 gaaaagctgg atgtgcttaa gcaactagag gaacgccgta aaagtttaaa aagctttaaa
      841 taaagtaatt gaaaaagagg tgcaaaagaa aatgtaacct ctttttaagt actattattt
      901 tctgaaaata gggttaatga ctgaaaaaaa tgttgatttt gtgaaatgat tattttatta
      961 ttttcatatt ttatctttat gctaggaatt gtggtataat ggaggagtat aaaaaatatt
     1021 aaggagtcta ttcacatggc taaattgact gttaaagacg ttgatttgaa aggtaaaaaa
     1081 gtcctcgttc gtgttgactt taatgtgcct ttgaaagacg gcgttatcac taacgacaac
     1141 cgtatcactg cggctcttcc aacaatcaag tatatcatcg aacaaggtgg tcgtgctatc
     1201 ctcttctctc accttggacg tgttaaagaa gaagctgaca aagaaggaaa atcacttgca
     1261 ccggtagctg ctgatttagc tgctaaactt ggtcaagatg ttgtattccc aggtgttact
     1321 cgtggttcaa aattagaaga agcaatcaat gctttggaag atggacaagt tcttttggtt
     1381 gaaaacactc gttttgaaga tgttgacggt aagaaagaat ctaagaatga cgaagaactt
     1441 ggtaaatact gggcttcact tggagatgga atcttcgtta acgatgcatt tggtacagca
     1501 caccgtgctc acgcatcaaa cgtaggtatt tcagcaaacg ttgaaaaagc tgtagctggt
     1561 ttccttcttg aaaacgaaat tgcttacatc caagaagcag ttgaaactcc agaacgtcca
     1621 ttcgtagcta ttcttggtgg ctcaaaagtt tctgataaga ttggtgttat cgaaaatctt
     1681 cttgaaaaag ctgataaagt tcttatcggt ggtggtatga cttacacatt ctacaaagct
     1741 caaggtatcg aaatcggtaa ctcacttgta gaagaagaca aattggatgt tgctaaagac
     1801 ctccttgaaa aatcaaatgg taaattgatc ttgccagttg actcgaaaga agcaaacgca
     1861 tttgctggtt acactgaagt tcgcgacact gaaggtgaag cagtttcaga aggcttcctt
     1921 ggtcttgaca tcggtcctaa atcaatcgct gagtttgacc aagcacttac tggtgctaaa
     1981 acagttgttt ggaacggacc tatgggtgtc tttgaaaacc ctgacttcca agctggtaca
     2041 atcggtgtca tggacgctat cgttaaacaa ccaggcgtta aatcaatcat cggtggtggt
     2101 gattcagcag cagctgctat caaccttggc cgtgctgaca aattctcatg gatcagtacc
     2161 ggcggaggcg ctagcatgga acttctcgag ggtaaagtat taccaggttt ggcagcattg
     2221 actgaaaaat aatagtcatt ataatatata gtaaagggca tgctgatgca tgtctttttt
     2281 tagttgatca tgtacagtta ttgaactttc cctaacttca cttatcattt actagttttt
     2341 agatatagtg gtatttggct gacactgcaa ttttcaaaaa tttatgacga tttcatatca
     2401 attggtttat aaaagatatc ctaatcataa ataaaacgtt taccaaaagg gttttatgga
     2461 ttgatatgtt aaaatagata gtgaagggat aggtatgaaa acgttaagaa aattattatc
     2521 taattacaag tttgatatta aaaagtttaa attgggaatg agaacattga aaacaggctt
     2581 atcggtcttt ttagttttat tggtttttca tttatttggt tggaaagggc tccaaattgg
     2641 tgctctaact gctgtattta gtttaagaga agactttgat aaaagcgtgc actttggctt
     2701 ttctagaatt attggtaata gtataggtgg cctactctcc ttagtatttt ttgcattcaa
     2761 tgagatattt catcaagctt tttgggttac tttattaatt gttcctattt gtaccatgtt
     2821 atgtatcatg gttaatgttg catgtaataa taaatcaggt attattgggg cagtggctgc
     2881 tcttttgatt attacactgt ctattccaac aggacaaacg tttatttatg tgacttcgcg
     2941 ggtatttgaa acattttgtg gcgtctttgt agctatttta gttaatactg atgttgaact
     3001 tattaaaaat aaatggttta acaaaaaaca cagtgatgtt agataatata acatatatac
     3061 ttgacaggtt atttttttca gcctataata aggaagatga aaggagtttg tcatgaaaga
     3121 aaaagaactt agacgctcaa tggctgtttt tcctattgga acggtgatga cattaacaga
     3181 tctctctgcg agacaaattc gctattatga agatcaaggt ttaattaagc ctgaacgaac
     3241 gcaagggaat cggcgtatgt tttccttaaa tgacatggac cgacttttgg aaattaaaga
     3301 ttttttgtct gagggattaa atattgcagc tattaaacgc gaatatgtcg agcgtcaagg
     3361 taagttaatg caaaaacaaa aagctctaac agatgcagat gtcagacgga tcttacatga
     3421 tgaaatgctg acacaaagcg gtttttctac tccctcacag catattggta attttcgtat
     3481 ttaagatatg aattgtataa aatttaaagg agacctattt aatggcaatc acagtagctg
     3541 acattcgtca tgaagtcaaa gaaaaaaatg taacgtttct tcgcttgatg ttcactgata
     3601 tcatgggcgt tatgaaaaat gtggagattc ctgcaacgaa agaacagtta gacaaagtat
     3661 tgtctaacaa ggttatgttt gatggttcat ctatcgaagg ttttgtacgg atcaatgagt
     3721 cagatatgta cctttacccc gatttagaca cttggattgt ttttccctgg ggagatgaaa
     3781 atggagcagt tgcaggttta atttgtgata tttatacagc agaaggaaag ccttttgcag
     3841 gagatcctag aggaaattta aaaagagccc tgaaacacat gaacgagatc ggctacaaat
     3901 catttaatct tgggccagaa ccagaatttt tcctttttaa gatggatgat aaaggtaatc
     3961 cgacacttga agttaacgat aatggtggtt attttgattt agcgccaatt gacttagcag
     4021 acaacacgcg ccgtgaaatt gtgaatattt taacgaaaat gggttttgaa gtggaagcta
     4081 gtcatcatga agtggctgtt ggtcaacatg agattgattt taaatatgca gatgttttga
     4141 aagcttgtga taatattcaa atttttaagc tagttgtaaa aacgattgcc cgtgaacatg
     4201 gactttatgc tacttttatg gctaaaccaa aatttggaat agctggatca gggatgcact
     4261 gtaacatgtc tttgtttgat aaccaaggta ataatgcttt ttatgatgaa actgataagc
     4321 gaggaatgca gctatcagaa gatgcttatt atttcttggg aggactaatg aagcatgctt
     4381 ataactacac tgctatcact aaccctacag tgaattctta caaacgatta gttccgggtt
     4441 atgaggcacc tgtttatgtc gcttgggctg gaagtaatcg ttcaccactt atccgtgttc
     4501 cagcatcacg tggtatggga acgcgtttgg agttacgttc ggttgatccg acagctaatc
     4561 cttatttagc cttggctgtt ctcttggaag ctggattaga tggtatcatt aacaaaattg
     4621 aagctccaga acccattgaa gctaacattt ataccatgac aatggaagaa cgaaatgaag
     4681 caggcattat tgatttgcca tcaacgcttc ataatgcctt aaaagctctt caaaaagatg
     4741 atgtggtaca aaaggcacta ggttaccata tctacactaa tttcttagaa gcaaaacgaa
     4801 ttgaatggtc ttcctatgca acttttattt ctcaatggga aattgaccat tatattcata
     4861 attattagac ttatcaggac ctgctcaagt tgagtgggtc ttttggatga gctattgtat
     4921 ttgagctagc aagctattat tagctgatcg gacaccaatt gattttccat tatagaaaac
     4981 taaaaaccag tccatgttct atgaggactg gtttttagtt tgataattat ctgaactaga
     5041 actgatatgt taaagatata gacagatagt attaagaaat gagtcttatt tgattttagt
     5101 gcttatcagg tgttaagacc atcggaatta taattggttc acgctcagtc ttttcataca
     5161 agaatggacg aagagcattg acgatagcac cgttaactga ttggatgcta gcatccttat
     5221 tttttaaagc aatacggata gcgttaaaga gcacacgttg gctttcgcgg atcaagtcac
     5281 ctgactcacg catataaatg aaaccacggc tcagaatatc tggtccagct aaaatcattt
     5341 gtgtgttaaa atcaacagtt gcaacagcta agacaacacc atcttctgac aaatcacggc
     5401 ggtcccgtaa aacggctgct ccaatatccc cgatgccatt accatccacg tagatgtctt
     5461 gggcattaaa gtgaccagca atacgcgcac tgtcactggt taaagctaga acgtctccat
     5521 tttccataat aaagatattt tcttttggaa taccaatatc catcgccagt cctgcatgaa
     5581 ctttttgcat gcgatactca ccatgtactg gcatgaaata ttttggttta atcaaactaa
     5641 gcatgagctt ttgttcttgc tgtcctccat gaccagaagt atgaatatta ttgacttttc
     5701 catgaataac atcaacacca gcttcttgga tggtattgat taatttattg acacttgtgg
     5761 tattaccagg gattggactt gatgagaaaa tcacagtatc accaggctgt aaagttactt
     5821 gacgatgagt gccattagcg atacgagcaa gtgctgccat agactcacct tgactacctg
     5881 tacacataat taaaacttca ctagcatgaa gatttttaag ttcgcttggc tcaatgaagg
     5941 taccttttgg aactttaata tagccaagtt caataccgtt aacaattgct ttttccatag
     6001 aacgaccaaa aacggcaatt ttacgtcccg ttttaacagc agcttctgca gcctgttgca
     6061 aacggtagat gtttgaagcg aaggaagcaa aaataatacg cccatgaata ccttcgataa
     6121 tttttaaaat ggattggcca acaacttttt cagagttagt aaaggttggg atttcagcat
     6181 tagttgagtc tgataataag caaaggacac cttcttcacc aagcgctgcc atacgttgta
     6241 aatctgcagg atctcctact ggcgtgaagt caaatttaaa atcaccagta cagataatct
     6301 taccttgagg ggtgtgaata acaataccga ccggttctgg aatggaatga gtagtcttga
     6361 agaaagtgac gctcatgttt ttaaaagtta actcggtgtt gtgattgatt tcgtaaacag
     6421 ttgcttcacg ccaaagacca tgttcttcca atttaccacg gataagagca agtgctaaag
     6481 gtcctgcgta gatagggata ttagcctgtt tgagaagaaa tggaatacca ccaatgtggt
     6541 cttcgtgacc atgtgtaata acaagagctt tgacccggtc taagttatcg acaatgtaag
     6601 aatagtctgg aataacgtaa tcaattccca aaagatcatc ttctgggaat ttgataccgg
     6661 catcaacgat aatgatctcg tcttgatact caataccata agtattcttc ccaatctctc
     6721 ccagaccacc aatggcaaac accccaactt cattaggttt taaactgata tttgtcatct
     6781 tagaactccg ttaattcaaa aacaccggtc tctttttcgt aatcgaggtg tttgtcagaa
     6841 agtagctcga taaattccac gttataatag gtgttatctt caacgagacg acgagccttg
     6901 atacggccat cgagttcatc agtagcatca atattaagat agagtgcttt agtactttca
     6961 cgacgtgggc tttgatcctt tgtttcttga tagaaaactt tataaatcat ttgttttttt
     7021 tcctttcgtt atgaatcaat tatccaaaca ataataaata ggtcaaaagg taggtgttcc
     7081 attttgacgg ttttatatgt tgtcattatt gtgtagtaga ttaattcctg attctgtgca
     7141 attattgcaa ttataccata aacttagctt ttagtaaaaa atttaggtac atttatggta
     7201 tagtctaaat caaattaaga gtcagccttt ttgtttatga ttcttaattt gtgttataat
     7261 tgatactaga acttgtagta gaaaggcata aaatgaagac acttgcattt gatacctcaa
     7321 ataaaacctt gtcccttgct atacttgatg atgagacact tctagcagat atgaccctta
     7381 acattcagaa aaatcatagt gttagcctta tgcctgctat tgattttttg atgacttgta
     7441 ctgatcttaa acctcaagat ttagaaagaa tagtggttgc aaaaggccct ggatcttaca
     7501 caggtttacg agtggcagtt gctactgcaa aaacgttagc gtacagttta aatattgcat
     7561 tggtcgggat ttcgagtcta tatgctttgg ctgcgtctac ttgtaaacag tatccaaata
     7621 ctttggtggt gccattgatt gatgctagaa ggcaaaatgc gtatgtaggt tattatcggc
     7681 aaggaaaatc agtgatgcca caagcccatg ctccactaga agttattata gaacaattag
     7741 tagaagaagg gcagctgatt tttgttgggg agactgctcc ttttgctgag caaattcaaa
     7801 agaaactacc tcaggcgata ctacttccaa ccctgccttc tgcttacgaa tgtggtcttt
     7861 tggggcaaag tttggcacca gaaaatgtag acgcctttgt ccctcaatat ctcaagagag
     7921 tggaagctga agaaaactgg ctcaaagata atgagataaa agatgatagt cactacgtta
     7981 agcgaatcta atatgaaaac agttgaagaa caagcaaaaa atatttatca gcttttggaa
     8041 atggtttatg gcacgtctcc ttggacttta gaacaagtat taatcgatat atggcgggat
     8101 caaacagatt atttcctact atatgatcac gataaattgc taggtttttt agctattcaa
     8161 gatttagctg gtgaagttga aatgactcaa attgctatac tacctagtca tcaaggactt
     8221 gggctagcgt ctcaattgat gacgcattta ggtagcattg aaagtgacat ctttttagag
     8281 gttagagaat ctaatcatag agcacaaggt ttatatcaga agtttggttt taagtttatt
     8341 ggcaaaagac cagactacta tagaaatcct atagaaacag ctttattgat gaaacgggaa
     8401 ggaaaaaatg acagataggt atattttagc agttgagagt tcttgtgatg agaccagtgt
     8461 agcgatttta aaaaacgaaa gtaccctctt aagtaatgtc attgcaagtc aggttgagag
     8521 ccacaaacgc tttggtggtg tggtgccaga agtagctagt agacaccatg tcgaagtgat
     8581 tacgacttgc tttgaggatg ctttgcaaga agctggcatc agtgcttcag atttatcagc
     8641 agttgcggta acttatggtc ctggacttgt tggagccttg ttagttggat tagcagctgc
     8701 gaaggctttt gcttgggcaa atcatttacc tttgattccg gttaatcata tggcaggtca
     8761 cttaatggca gcgcgtgaac aaaagccttt agtttatcct ttaatagcac tgttggtttc
     8821 aggaggacac acagaattgg tttatgtccc agagccaggg gattatcata ttattggcga
     8881 gacgcgtgac gacgctgttg gtgaggctta tgataaagtt gggcgtgtga tgggcttaac
     8941 ctatccggca ggacgtgaaa ttgaccagtt ggctcataaa ggtcaagata cttatcattt
     9001 ccctcgtgct atgataacag aagaccactt agaattttct ttttcaggac tcaagtccgc
     9061 ttttatcaat cttcatcata atgctaagca aaaaggcgat gaactgattc tagaagattt
     9121 gtgtgcatct tttcaagcag ctgttctaga tattttattg gccaaaacaa aaaaagctct
     9181 gagcagatac cctgcaaaga tgcttgtagt ggctggtggt gtagcggcaa atcaaggatt
     9241 acgagataga ttagcacaag aaattacaca tatagaagtt gtgattccta aactaagact
     9301 ctgtggagat aacgcgggta tgattgcttt agcagcagct attgagtatg acaaacagca
     9361 ttttgctaat atgagcctaa acgcgaagcc tagtttagct tttgaccagt ttccagacag
     9421 ttttgtaata aactagtcac acttgctagt tagtaagatt tataatggat aagaatctcc
     9481 ctttgaaaaa acaacccggt atcttggcat accgggttgt catgatgata agtaattggc
     9541 ttctatggtt actaataatt cagataatta cctgcaaaat aattgtctgc ctaaggtgaa
     9601 gtaagcttac cagctagctt tagtgacgcc aggaagctgt cctttgtgag ctaagtcacg
     9661 gaaattgata cggctaacac caaacttacg catgtaagca tgaggacggc cgtcaatttt
     9721 gtcgcggttc ttcaagcggt ttggatttga atcacgaggc aatttgcgca gtgattcata
     9781 atcgcccttc gcttttaagt cacgacgaag gtcagcgtac tgttcaatca gttggagctg
     9841 cttttggtat ttagcaattt ttgacttttt tgccattgct ttctccttct taatatttaa
     9901 taacctcaga gtcgttatca gcgctctgaa tccacttaac tggtatttaa ctggttaagt
     9961 ggatatataa tcattataca ataaataaaa gcaaaagcaa ataaaaaaaa ttaactagtt
    10021 atctttttta gccataatat caaaaaactc cacatctaag tgtgggagtc aggtattaat
    10081 aactgtttaa aggtgtttaa tgagtgtcgc tttggcaact ttgagattga acttttaagt
    10141 caaataaatc agcaggaacg tagtgtttgg ttgagaccaa gatgtcttta ccattgtaga
    10201 gagtttcttg gattaaaata aaagcactat tttctgatag agtatactta gttgctgtgt
    10261 aattgccagt cttggtatag ccgatttggt tgcaggaatg agaagcttta ccagattcat
    10321 agattttctc ttctaagaaa gtaagaaggg gctcttcttg attgagattt atagcatatt
    10381 tagaaataac ctcaataggg ataaaagata aactataagc aatagcttta ttttggctat
    10441 gataccagcg atcaacaata atcacaacag gagtttcttg ttttagggat gctgtaatgg
    10501 caacagtggg cacttcaatc cgatattcca attcaacctt tgtgatgtca gatgataggc
    10561 ttgcatatat ggggtgttga agatattcga aaccttggtg atatttagtt tcaggagtct
    10621 tgagtataaa atttcccttt cctctgatat tttttatgag atgatcctct tggaggaggg
    10681 ctagggattt acgtagtgtc atccgactga cattgagctg tctagcaagt tcaggttctg
    10741 atggcagttg catgccagga ctatatgtac catcttgaat aagttgaaaa atagtattat
    10801 atacttgcac atgtttaagt tttttgagtt ttttggttaa gtcgttagta gacatatcac
    10861 ttacctcatt taataatctt tagtggagtt taacttgtct aaaaactatt ttagcactaa
    10921 cagtatttct tgattagaat tgtacccatc tatagtgtta gtatctcaca ctcaggtact
    10981 ttttgtcaaa aaagagatga aaaacacctt aaaaaagcgt ttacaaaata gagtgtgtgt
    11041 gatactatac aagtgtatca aaaattagac aagtaaaata caggaggttt ttaacatgca
    11101 aaattattca ggtgaagtcg gattgcaata tcacctacag attcgtccag gtgatgtagg
    11161 tcgttatgtt attatgccag gtgatccaaa acgttgtgca aaaattgcag aacattttga
    11221 taatgcagtt cttgtagcag acagccgtga atatgttacg tatacaggta cattaaacgg
    11281 tgaaaaagtt agtgttactt caactggtat tggtggtcca tcagcgtcta ttgcgatgga
    11341 agaattgaaa ctctgtggcg ctgatacctt tatccgtgtt ggaacatgtg gtggtattga
    11401 ccttgatgtt aaaggcggag acatcgttat tgcaactgga gctatccgta tggaaggtac
    11461 cagtaaagaa tatgctccga ttgaatttcc tgctgtagct gacttggaag tgacaaatgc
    11521 tctagttaat gcagctaaaa agcttggtta tacaagtcac gcaggagtag ttcaatgtaa
    11581 agatgcattt tacgggcaac atgagccaga acgtatgcca gttagttatg aattattaaa
    11641 taaatgggaa gcatggaaac gtctgggaac aaaagcgtct gaaatggaat ctgcagctct
    11701 ttttgtagca gcaagtcatc taggagttcg ttgtggatca gatttccttg ttgttggtaa
    11761 tcaagaacgc aacgctttgg gtatggataa cccaatggct catgatacag aagcagctat
    11821 tcaagttgct gttgaagcac tccgcacact tattgaaaat gataaatcac aataagcgag
    11881 gttttggaag tcatcatgca atttatttat agtattattg gtattttatt ggtattagga
    11941 attgtgtatg caatttcttt caatcgtaag agtgtttctc taagtttaat tggaaaagct
    12001 cttatcgttc aattcattat tgcgctaatc ttagtacgta tcccactagg ccaacaaatt
    12061 gttagtgttg tttcaactgg agttactagc gtaatcaact gtggtcaagc tggtttaaat
    12121 tttgtgtttg ggtcattagc agatagtggc gcaaaaactg gttttatttt cgctattcaa
    12181 acgcttggta atattgtttt cttatctgcc ctagttagtc tactttatta tgtgggaatc
    12241 cttggatttg tagtaaaatg gataggtaag ggcgttggta aaattatgaa atcctcagag
    12301 gttgagagtt ttgttgccgt agctaatatg tttcttggtc aaacagacag tccaattttg
    12361 gttagcaaat acctaggtcg tatgactgat agtgagataa tggttgtgtt ggtatcaggt
    12421 atgggaagta tgtcagtttc tattcttggt ggctacattg cattaggcat tccaatggaa
    12481 tatctcttga ttgcttcaac aatggttcct attggcagta ttctcattgc taaaatctta
    12541 ttgcctcaaa cagaacctgt tcaaaaaatt gatgacatta agatggataa taaaggtaat
    12601 aatgctaatg tgattgatgc aatcgctgag ggtgcaagca caggtgcaca aatggctttc
    12661 tcaattggtg ctagtttgat tgcctttgtt ggtttagttt ctttgattaa tatgatgtta
    12721 agtggattgg gaatccgctt agaacaaatc ttttcatatg tttttgctcc atttggtttt
    12781 cttatgggat ttgaccacaa aaacattctt ctagaaggaa accttcttgg aagtaagttg
    12841 attttaaatg agtttgtttc gttccaacaa ttgggtcacc taatcaaatc tttagattat
    12901 cgtacagcat tggtagcaac tatttcactc tgtggttttg ctaatttatc aagtttaggt
    12961 atttgtgttt caggtattgc tgttctttgc ccggagaaac gtagcaccct agctcgactt
    13021 gttttccgtg caatgattgg tggtattgct gtaagtatgc ttagcgcctt tatcgtcggt
    13081 attgtaactc tattctaaac gttgacaaaa gaaagaagga tagtaacgtg gaagtaaaag
    13141 atattttaaa aacggtagac catactttgc tagcaacaac agcaacgtgg ccagaaatcc
    13201 aaacaatttt agatgatgcc atggcttatg aaacagcttc agcatgtatt ccagcctctt
    13261 acgtcaaaaa agcagcagaa tacgtttcag gtaaattagc tatttgtact gttattgggt
    13321 tcccaaatgg ctatagtaca actgcagcga aggtttttga atgtgaagat gctattaaaa
    13381 atggtgctga tgaaattgac atggtcatta atttgacaga cgttaaaaat ggggattttg
    13441 atactgttga agaagaaatt cgtcaaatca aagctaaatg tcaagaccat atcttaaaag
    13501 ttatcgttga gacatgtcaa ttaactaaag aagaacttat cgaactttgt ggagttgtca
    13561 cacgttcagg tgcagacttt attaaaacct ctactggttt ttcgacagca ggtgctacat
    13621 ttgaagatgt tgaagtgatg gcaaaatatg tcggcgaagg tgttaaaatt aaggcagcag
    13681 gtggaatctc atcattggaa gatgctgaaa catttattgc tttaggagct tcacgcttgg
    13741 gtactagccg tatcatcaag attgttaaga acgaagctac aaaacccgat agctattaac
    13801 ttaatcatac cgatttagct tgagactaaa acaaaaaact catgtaatag gagttaaaaa
    13861 gcctactatc ttagtgataa taggcttttt tagctatgaa gacttccctg cgatccatcc
    13921 tgaacaaaga gctgaggtaa tattaaaccc tccggtgtga gcattaatat ctaagacctc
    13981 tccagcgaaa tacaggccag gaactttctt actttccaag gttttaggat tgatttcttt
    14041 taaatccacg cctcctttgg tcacaaaaga tttagccaaa gacatttttc ctgtaatggg
    14101 tatctggagg tgtttgagtt tatcaagcaa ttccttttct tgttttggag agagttgttt
    14161 gaccttttcg gggtaatctt ctgataaaaa atcagctact cgttcaggca gtaggccttt
    14221 aagagcattt ttgatgtttt tatcacgttg atcactcaga taagcggtta agtcatctgt
    14281 tgataagtgt ggcaaaaagt ccagctctgc aatttcccca cctttcacaa aagaggacag
    14341 acgaagagct gcaggtcctg aaaggccaaa gtgggtgaag agtaagtcgt gtgtgatgac
    14401 gtgtttgtca taactaagag taacgtcgtc tagggaaata ccttgcaaga ctttgtgagg
    14461 aaaatcagtt aacagagggc tctcagcagc ttctaaatca gtcactgtca atttaaaatg
    14521 ccgtgcaata tcatgtccaa aacctgttga acccgttgaa gggtaggact tgcctcctgt
    14581 ggtcacaact aatttatgac agctaaaagt ctggtctgca gatttgaggt agaagaggtc
    14641 atcttgtttt ttaacggaaa ccacttcagt gctggttagt accttgcctc caagagcctt
    14701 gatttttttc tccagagcat caatgattgt cctagattta tctgttgtgg ggaacatccg
    14761 tccatggtct tcttctttga gtctgacgcc gttttcctcg aaaaaggcaa tgatgtcatg
    14821 gttgtcaaac tgggaaaaaa cactatataa aaagcggcca tttccaggaa tgcctgccat
    14881 aaggtcatca aggttaccac tattagtgac attacagcgt ccaccaccgg ttcccgctaa
    14941 ttttttgcct aagcggcgat ttttttcaat taagagagtt ttatagccat agtagctgct
    15001 ggagatggcg gccatcatac cagcagggcc acctccaata ataatcgtat cgtattgagt
    15061 catagcctta ttttagcata aaagcggggg atttaatagt caggtaaatg aactaacctg
    15121 gcataccgtt ttctattcat ctaacaatgt tttcaagaaa taattgatgc ctttagcacg
    15181 actgaaatga ggtcaaattg acagaatact cctattatgg tactattttt aataagaata
    15241 ttcagtgata gtgataaaga ggaaaatatg ggaattgaaa agacagttag tgaactagct
    15301 gatattttgg gagtgagtcg tcaggccgtc aataatcgtg ttaagtcttt acccgaagaa
    15361 gatcttgaca aaaatgaaaa aggcgttact gtggttaagc gcagtggtct tgttaagctg
    15421 gaagagatct ataaaaaaac aatttttgac gatgagccta tcagtgaaga aacgaaacaa
    15481 cgtgagcttt tagagattct tgtggatgag aagaacactg aaatcacgcg cctttatgag
    15541 caactcaaag ccaaagatgc tcaacttgcc tcaaaagatg agcaaatgcg tgttaaagac
    15601 gtgcagatcg ctgaaaaaga taaacaattg gatcagcagc aacaattaac tgctaaagcc
    15661 atggctgata aagaaaccct aaaactagaa ttggaagaag ctaaagcaga agccaaccaa
    15721 gctcgcttac aagtcgaaga agttcaggct gaagttggcc ctaaaaaagg ctttttaacc
    15781 cgcttgtttg cgaaataatc acaaaaaatg acgaaggccc tatcagggtc ttttttggag
    15841 gaaaacatga aacacttaga tacttatatt gcctttgatt tagaatttaa cactgtcaat
    15901 gacgttagtc atatcattca agtatcagca gtcaaatatg accaccataa agaagttgac
    15961 agctttgaca cctatgttta cacagatgtt cccttacaga gctttatcaa tggcctaaca
    16021 ggaatcacat ctgacaagat tgctgcggca ccaaaagtag aggaggttat ggctgcgttt
    16081 aaaaattttg tgggagagtt accactgatt ggttataatg ctcaaaaatc tgacctgcct
    16141 attctagctg aaaatggctt ggatttaagg gaccaatacc agattgacct ctttgatgaa
    16201 gcttacgata gacgtagcgc agacttaaat gggattgcta accttcgtct gcaaacagtg
    16261 gcaacctttt taggcattaa gggtcgaggt cataatagct tagaagatgc cagaatgaca
    16321 gcagcaatct ataagtcttt tttagaaaca gatacaaaca aagcatatct cagccagcaa
    16381 gaagaggtaa ccacagataa ccctttcgca gctttgggag actttttcga ctaaaggctc
    16441 tgctattttt gaaaaataaa aaaatcccta gggttagccc ttgcttataa cgctaggtca
    16501 tgtgataagc tagggacata tcaagaggga gtcgagtgca cacgaatcgt ttttgatcac
    16561 taaatgatag aaagaaggtg tggaaatgtc tcaattctat tcgactggag aactggccaa
    16621 cttagctggc gtttctattc gaactgtcca atactatgac caacgtggga ttttgattcc
    16681 gacagcattg acagcaggtg gacggcgact atatacggat tctgatttag aacaattacg
    16741 catgatttgc tttctgcgcg atttgggatt ttccattgag cagattcgca aagtcttagc
    16801 agaggaaaat gcagcccagg tgctggaatt gcttctagtt gatcatattg cgactgccaa
    16861 ggaagacttg gccgcaaaag agcaacaagt tgacatagct gtcaagatac ttgacagact
    16921 tcgaaaacaa gacccccagt cactagattt tctgatggac atttcgctta gcatgaaaaa
    16981 tcaaaaagct tggaaaaaat tacaatggaa gatgggtatc agtatatttt tagccattgt
    17041 ttcatatgta ggacttattt tactatctaa ttatctgcaa aaattttggt tagtttatgt
    17101 tgctatggga ctatttctac caggtttcag ttggttggta atttatttta ggaagcaatt
    17161 tatttatctc tgtccccatt gtcatcagaa atttgaacct agctttaatg aatttgccct
    17221 tgcaggccat acaccacgaa ccagaaagtt aacctgctcc cattgccatc aaaaatcgta
    17281 ttgcttggaa ctggcaaaag aggacactgt ctcaagagga taagcactaa ctcataaaaa
    17341 aactagcctg agatccttct caagctagtt tttgtttttt tagaatggta atttaccagc
    17401 aaaagcgcct aatgttttct tagttgcttc atcaatttgc gtaagggcat cattaatggc
    17461 ttgtgtggtc atgtcttgaa gcgtttctac atcttcagga tctacaacag cttctttgaa
    17521 gtcaatccca accaattttt tatcacccgt gaaagtggca gtgacaaggt cttgagctga
    17581 ttttccagta aattgcatag cagccaaatc cgcctgcttt tgctccattt gtttctgaag
    17641 cttttgtgcc tgcctcatca tattctgcat attcatcata agactatact ctccttaatt
    17701 taatactaac tattataaca tataaaaaga ggcaataaaa acaatgtcag ttagtagact
    17761 agcctgtatc agtaagaaag tcgctatgga taagatggat tagcagcaag ggtctggcta
    17821 atgacagaat aagtctagaa atgaactatt tttgtcatag actagtccga cttagtggtt
    17881 atctaattca cgatcttctt tagcttgttg ttctaaaaga atttgtgcga agtcaaccca
    17941 cttagtgtag ttattttcgg ataattgttc tgaaatcatc gctaactctc gttgtggcaa
    18001 ctgataagag actcccttac tttctaaggt taaatcttct tccattaggg cggttaaact
    18061 aacatcaaag atagcagcga tatcacttaa tagaccagac tttggagtat attttccttt
    18121 ttcccactca ctgattgaag aagagctttt tcgtcctagg agattagcaa gttctaattg
    18181 ttccatgttc ttttttaatc taaggtactt taaattactt gcaaaaaaag ttttcttggc
    18241 ggacatattt ttctcctttt ctttatataa aatatgatac cataaaagag gttaaagcaa
    18301 aaggtatcgt aaaagatggt ctaaaaactc tctaaaaact tgacttcata aaaaccgaag
    18361 tttataataa acttaacatg attaactaat ctcattttag tgtggaggga aaattgtgac
    18421 caaatcaact ttaaaagagt taagaacaac aaagaaaatg acacagcagg aattgtctca
    18481 cttaacaggt atttctgtca gaacgattgc taggtatgaa aaagatacta aaaagctaag
    18541 gcgagcaaaa tatgagaagc taaaaggtat tgcacaagca ttagcggttt ccgttgatga
    18601 tattttttta ggaatagatt cggaatttat gaactgagac gatcgttttc tgtttgctat
    18661 tagtaagacc aattaacaaa aaagtgctta gttatattgc taagcacttt tctttgttaa
    18721 tttgatgcta ttggcacaat caattgagac tgactgagat caatagttaa agtatagttg
    18781 ctgttgtcac ggacagtatg ttcaaaatcg gttgtgtata agatgactcg aagagtattt
    18841 cctttttcta gatgatagat agttggttgc aaaggaagtc taacagtcat ccatttatta
    18901 ttaggaatag tttcaattga cgacaaatta ttgcgatttt gtaaattcat gaaacctttc
    18961 gaaataacac ggtaagaatc ttctctaaat ggtagctctt taaggggttc tctagagaaa
    19021 ttttgcccat tgtcaatact tgattgggtg agagcaatgg gaagatcacc caagcgtttt
    19081 ttcttaccat agtcaaggat ttgtgcagat agaagtcctt tattttcact tgatttcact
    19141 tttaactgta ggacaatttc accgttaata ggtaagtcct cttccagtaa aatatcaacc
    19201 agggcttgat tggccttgcc tttaaagaga gctgctttga acgcacggaa atctttaccg
    19261 taagctttaa attcatcttc accatagtga ttatcaatta aaagcagttc ctgaccgagg
    19321 tcaagctctt tgatggttga ggtttcaaag actttacgct cctgccagtt ttgtgggcag
    19381 gtgttatcct gccagatcat ttcaggaagg ctaaaatcat tagctaggcc taagagtttt
    19441 tgacataaaa gggcattcat gctttcgcga aaatcaatgg attgccaatt atgcatgtaa
    19501 acatgctcac cttgatgtaa aaagaggtgc ttgttgattg tggaaggaag cgcattgaca
    19561 atctcataga cctgtcttgg tttgacattc cagtcttgta agccatgagt ataaacgaca
    19621 tcacacttta tttgatgagc attttttaag taattgcggt cgtgccaaaa ttgattataa
    19681 tcaccagatt gtcggtctaa cgcttgagac tgttggttta gcaattcttg ataacgatca
    19741 ttatggcgaa gataatcgcc agccaataaa ttacgcgagt aagtgagctc agttaggaca
    19801 tctagatcct ctccaggata gccaccagga ctacaaacta agccattttc gcggtagtag
    19861 ttgtaccaag aagaaatagc tgattctgca ataatcatgg ctaatccgtc tacgcctgta
    19921 gtggctaaac cagtagacat agttcccaag taggactttc cagtcgtaca aactagacca
    19981 tttgcccaat cagctctcac ttggtgggtt tttgagtggc tggtgtaggc tgtggctctt
    20041 ccatttagcc aatcaataac ggctttaaag ctttcaattt gagcatagtc tccactcgtc
    20101 ataaagccag tggaaccagc agttcccaca cctgaaacat agatattagc aaacccacgt
    20161 gcgaggaaat aatcgttgag gctataggag ttaatgtagc taaagctttc tagattttga
    20221 cctataggaa gtttgcaagg ctgtgtctca agaggaataa aatccctgtc ttcgacagtg
    20281 atttgtctcc tttttttaac gactaactct ttttccatac gataaagttt tttgtcgttg
    20341 gctacttcat tgataccttg atggtatgga ctaggtgtca tcaaagttgg taatggtttt
    20401 tgagattgag gacgaatgat attagcctta ataagatcaa gttggccatc ttgatcggta
    20461 tcaagaggtg cttcaacgta aacaacttct cgaatcagct gtgacgtgtc aaaggtagct
    20521 aatgatttac cgttgaagaa atggtaatca ttgtccatag taagcattcc ttggctgacc
    20581 aaatcatcaa tcagggtcat gccagactta caacgggtcg ctaataagtg gtggatatgc
    20641 ttgataattt ctgtttcgtc aaagtggata ggaaaggcca gtttatcaag gaaagcatcg
    20701 gcctctgtaa aatcaactcc aggaataaag ccaaggactt gcagggccag taaatcaaaa
    20761 acctctttgg ataactcttg ctctgattga aaaaagctca atgcgtccat gtcatttttg
    20821 gcgattagat gacttaaagg ataatcacta tcagggtagt ggaagaagag tttacgtaaa
    20881 aagctttcta ggttagcttt ggcagttttt tgcagatcca gatcaaagcc caattctttt
    20941 aattcttcag ccgctctttc tagactagtt ggaatatagg aaaattgatt atagcgcata
    21001 ggagtttcct ttctagtggc ttagaaaacg atattatctt ttaatttatc aagattggta
    21061 atgataatat gttgtttatc aatgcgaatg atgttttttt ctttcaactg ttttagaata
    21121 cggctgacgc tgctagcggt agaaatgcca cagaatttac cgagttcctc attagtaatg
    21181 acaaaattaa ttaaaatttg gccatttggt agttcttcgc caaatagggt cattaagtca
    21241 tatatttgag tagaaatggc accgatacgg ccgttagtta acatacattg catttttttc
    21301 atggattttt gaagacgatt atggtaaaag tccttcacat atccttgtag ttcaatatct
    21361 tcgtttatat cttttaaaaa tgctgagcgg cgaaccttgt aaaaactagc tttctctgac
    21421 tcgatgcgaa cattataagg ctctcccata tcttttgaat aaccagtgtt gagaacagaa
    21481 gtaatttcaa gtcctgtaac atatctcaaa ttaaattcca ttccatattt tgaaagaaca
    21541 ctctgtttca caatgccgtc ttttaaaata taagtgaagt cctcttcgag actttcaaaa
    21601 gtcaagtact tgtgataact tttttcaatg ataggaaagt tatgattatc aatataccgt
    21661 tgcaaaatgt gatgattcac gcttttctcc aaatattctt ttaccaatgt ataatttgta
    21721 tcttcaagag aaattaacat ttgctaatgc cctttgatag taacacttat attataccat
    21781 tgaaatatat tatattctgc tttttttgta aaatgtttat gaaaatagag aggagttcat
    21841 tttcaatgga agaaaaattg tttaacaaac attttgtggc catcacagta ataaacttta
    21901 tagtctatat ggtctactat ctttttacgg ttattattgc ctttgtggcg actagagagc
    21961 taggagctca gactagccag gcaggattgg caacagggat ttatattcta gggactttat
    22021 tagcccgttt gatttttgga aagcagttgg aggtgtttgg tcgtcgtttg gttttaagag
    22081 gaggtgctat tttttatctc ttaacgacct tagcctactt ttatatgcca acgattagca
    22141 tgatgtactt ggttcgtttc ttgaacggat ttggttatgg tgtagtatca actgcgacaa
    22201 ataccattgt aacagcctat attccagctc gtaaaagagg agaaggaatt aatttttatg
    22261 gcttgtcaac cagtctagca gcagctattg gcccttttgt aggaaccttt atgttggata
    22321 atcttcatat tgattttcgc atgattattg tcttatgtag cgtcttgatt gggtgtgtgg
    22381 ttgttggtgc ttttgctttt ccagtcaaaa acatgtcttt aaatgcggag caattggcta
    22441 aaaccaaatc gtggactgtg gatagtttca ttgagaaaaa agccttgttt atcactgcaa
    22501 tcgccttttt aatggggatt gcctatgctt cagttttagg atttcaaaaa ttatacacgt
    22561 ctgaaattca tttgacaaca gtaggcgctt atttctttgt ggtctatgcc ttaatcatta
    22621 ccatcactcg tccagcaatg ggacgtttaa tggatgctaa aggcgataaa tgggtcttgt
    22681 atccaagtta tcttttttta gcaatgggac tcttcttgct aggtagtgta tcgtctggag
    22741 gaagctattt gctttcagga gccttgattg gctttggtta tggcactttt atgtcttgtg
    22801 gtcaagccgc ctccatacaa ggagtggatg agcatcgctt taatacggct atgtctactt
    22861 atatgattgg tcttgattta ggtttaggtg ctgggcctta cctcttgggc ttgattaaag
    22921 atttggcact tggtagtggt gtggcttcct ttaggcactt attctggtta gctgctgtga
    22981 ttccactgat ttgtaccttc ctttacttgt tgaaaacaaa gacaagacag gttgtgtctt
    23041 aaggacaaga aaaggagttt agtcttatac ttaaatacaa acgtatctta acagctgtag
    23101 atagttccta tgagccggga ttagctgtgg gtaaagccat tactgtagcg ctttgaaata
    23161 atcctagttt gctattcgta catgtcaata aaaatgactc gcaaaagcca ttcgtgaaag
    23221 agaaaatgtg gccttatttt tagtaggtgc aattggatta acatcagaat ataggatttg
    23281 tcatgttaag attgatctgt tgatagtgag agctaataaa aaatgttata atactatgaa
    23341 ataaagtgat aaagataagg tcttttatgg tccttttctt taccgctttt attattgtgt
    23401 gaaaataaca cttaatgatc attttattta ttattttatt acctttacat tcatcaataa
    23461 gcctgttaaa atggtatttg ttatatttta ttatttggag gaaaccagaa aacatggaaa
    23521 tgacatggac tgtgaaatac atcacagaat ttatcgcaac ggcttttttg attattttgg
    23581 ggaatggagc tgttgctaac gtcgacttaa aaggaacaaa aggacataac tcaggttggt
    23641 tagtgattgc ttttggctat ggcttaggag ttatgatgcc agctttgatg tttggtaatg
    23701 tatctggaaa ccatattaat cctgctttca cagttggact agcagtatca ggcttgttcc
    23761 catgggctca cgtcttacaa tacgttgtgg cacaattact tggagctatc tttggtcagt
    23821 tggtagtggt aatggtttat aaaccttact ttatgaaaac tgaaaatcca aatcatgttt
    23881 taggctcatt ctcaacgatt tcatctcttg ataatggtca aaaagacagc cataaagtct
    23941 cttatatcaa tggtttctta aacgaatttg taggttcatt tgttcttttc tttggtgctt
    24001 tggctttgac taaaaactac tttggggtag agttagttgg taaattgatt gaagcaggtt
    24061 acgaccaaac aactgcagca acacaaattt caccatacgt gacaggctca ttggcggttg
    24121 ctcacattgg tattggtttc ttagttatgg tcttggttac ttcattaggt ggaccaactg
    24181 ggccagcact aaacccagcg cgtgattttg ggcctcgttt gttacaccac ttcttaccaa
    24241 aatcagttct tggtcaagcc aaaggtgatt caaaatggtg gtatgcatgg gttcctgtag
    24301 ttgcacctat cttagcggcg atagtagccg tagcagcgtt taaatacctt tatatcagat
    24361 aaaaaaagaa ctaaagcttc ttttagactc aagacaagct cgcttaagga acttgtcttt
    24421 tttatatgag ttaagcgaaa tctaatgcaa acttgactgt cttgtatagc gtattttgta
    24481 aattttacta gacttcttgt aaaaaagcct ctcctcagat aagatgacta atgcctctaa
    24541 acggtgttac aaataaacga ggcatcccta acttccttta tctggtgttg aaactatttt
    24601 gcacggacta aatcactgat agagtaaaaa acgatttttc atttttgttg taaaatatta
    24661 aaaacgtctc tggttagtga ttcccagaaa cgttatctta tctatttgtt tgttgcttat
    24721 gagaggtaaa ggaggaacga atcagcattc caccagtgaa gaaaacgata gcgatgacca
    24781 gaagtttcaa gctattaata tctaaattcg ttaagaaaaa cacagctagt aaaacgccaa
    24841 taataccacc agaaacaatt ccagcaaaac catcccaact gacacggttt gctttgataa
    24901 actggctact agaagctgtc ataatcatag ctgcatctag catcataaca ggcatagcaa
    24961 ctgttgggct aagacccatt aatgaaaaga aaatcaattc tggagcataa ttgcctaacc
    25021 ccatagtcat caagactcca acgataaaat taaaaccaat tccaacaaaa agccagatgc
    25081 catggaggcc atgaatggtg tctgaaaggt gttctcctgg atggtagatt attctaatga
    25141 tcatgattat agctgctatg attaaaagac taccaaggat tcgttggaca gttggagcat
    25201 gccagttttt tgtaatatga gtcccaaaat aagctccaat aaaggctgct gcagccatag
    25261 caagtaaagt tagaacctca acttttacga caaaaataaa gcaaagagat tgaatgagca
    25321 ccggaagcac atgagctacg gtcatagtgc caggcaattg tctatcgtca gtgactaatt
    25381 tagttaactt aaagcacgtt gtggtagtag caaaagaccc tatcccaagg gtgtcaaggg
    25441 catctgttag caggccaatc caaaaccccg tccaaaagcg tttaaaagga ttgatcttat
    25501 tttttttaag atgttttact aacataaaga aaataaaaag cattgccgaa actagcaaag
    25561 cttgtatcag tctaaggata agcatttcgt tcataaagac tagtttacaa aaaaaaacct
    25621 tagccgtcaa cgtgatttac ttcattaaaa catgccacta tgcttagaag agctttagtt
    25681 cgatgtattt tgttaaaatt aagctaaaat attaatattg catgaaaggg gacgccttcc
    25741 tttttccctt actgagctta gctgttattg tagtgaatgt ttaaaaggct agtttgtcgc
    25801 tctttatcaa aaatctagta ggatgaatac gtataatttc ttgtcaaata gaggaatttt
    25861 gtctataata gcagaggatt aggaggaaat tatgacttta atggaaaata ttacctttaa
    25921 aactccagta ttacgcgtta atgatcgcga ccttaatatt gcattttatc aaaataattt
    25981 agggcttcgt ttagtatctg aagaaaatgc gattgctatc ttttcatctt ggggagaagg
    26041 gcaggaatgt tttgtaattg aagagtcacc ttcagttaga acgcgtgcgg tagaaggccc
    26101 taaaaaggtt aatacgattg tcatcaaaac aaatcaacct aaagaaatcg aacaattatt
    26161 agctcatgga gcacattatg acgctctttt taaaggtcaa aatggctatg cctttgagac
    26221 tatttcccca gaaggagacc gattcttgtt gcatgctgaa caagatatta aacatctcca
    26281 aggtactgat ttaccatcgc ttgaaaaaga tgcgactttt aaaggtttaa ctcaatttaa
    26341 gtttgatatt attgttttaa atgttattag tgaagaacgc tcaaaagctt tttatcggga
    26401 tctgtttagt gatcagttac ctattactat ggattttatt caagaagaag gtcccgattt
    26461 ggcaattgat ccccatattg cttgggattt agaaatttta gaatttcaag tctcaaagga
    26521 ctatgatatg aaggtcctaa aagcaacttt agaagaagac gggcacaagg tctacattga
    26581 taaaaaacac aaagtattgg tcctttctga tccaagtcag atcgaagtat ggtttacgaa
    26641 atgactttgg cagtcattaa atgtattgaa aaccatcttc acaaaaaagt atacaaaggg
    26701 gccagtctgg ctctctttca atctggtcga tggcaagaat accatatcgg aacaattgat
    26761 ggaagacgac cagttgatgc taacttagtt tatgacttgg ctagtgtttc aaaagttgtt
    26821 ggtgtggcca caatatgcaa tattttattg aataatggta cgttagcatt agatgatccc
    26881 ttaaaagtat attatcctag tattgctgat acgactgtca ctattcgaca gttattgact
    26941 cataccagtg gtcttgatcc ttatattcct aacagagatg ttttaaatgc acaacaatta
    27001 agaaaagtac ttaatcatct tactcaaaaa gaaaataaaa acttttatta cacagatgtt
    27061 aatttcctct tgttaggctt tatgttagaa gagctcttta gtgaatcttt agaccagata
    27121 tttgataaga ctatctttac tccctttggg atgtatcata cgtcttttgg ccctcgtcca
    27181 gaagctgtac caacattaaa gggtgtgagt gatggagaag ttcatgatcc caaagcaaaa
    27241 atcttaaaaa aacattctgg atctgcgggg ctattttcaa ctcttgcaga tttagaaagt
    27301 tttagcaatc attatcttaa tgatccattt tcagattgtc tgtggcgcaa ctacagccaa
    27361 caaacaattg agcggtcact agggtggaat ttagatggcg attggattag tcatacaggt
    27421 tacacagggc cgtttctaat gcttaataaa aaagagcaaa cggcagctat ttttttgacc
    27481 aaccgtactt acgacgagga tgataaaagt aaatggttaa aagaacgtca gttgctttac
    27541 aatgcactaa agcatgatct gacaacaccc gtatcgtaac gtctaaaggg gcagttttag
    27601 tgatcttatt actgttttta aagtttcttc taaaaaatag ccatttcatt tgtctgatgc
    27661 gattagagtg attataagtg aaaaaagaac agccatctca ttttgaatca aatgagatgg
    27721 ctgttctttt ttataagtta ttgatcattt agatactact atttacttga aatagcttca
    27781 gcggcatcat ccattgaaag cacttcgtgg aagacacgtt gagtcaattc agttttttgt
    27841 tctggtgtaa ggtatttggt gttgacacag taaccagaga tacgcacgat aacgtcttcg
    27901 ccgttcatga ttttgtcata aacatcgtta aggtccataa cgttcaagtt aacgtgctga
    27961 ccaccatttt caaagtaacc gtcaagcact gttaccaagt tgtcaacttg ttcgtcaaat
    28021 gttttaccaa gagcacgagg tgatacttga gtagtcaatg agataccatc gttagcatgt
    28081 gagaattcca gtttagcaag tgagttaagg ttttgtaacc agccaccttt tgctttgtta
    28141 gatgggttag cacctggtga gaagaattcc acttggctag tgttaactgt accatcttcg
    28201 ttcaagaaga cgccgcggtg aactggagag ttaccagttt gtttagagta agcaacgttt
    28261 gaagtgatgg taagaagtga tactgaagcc tcagcattct tgtaaagttt gtggcttgca
    28321 agacgtgtgt ggtaagcttc catcaaccat ttcgcgatgt catcaacacg gtcgtcatct
    28381 tcaccgtaac gagggaagtc acctgttact tcgtagtcat aaatgtagcc attttcatcg
    28441 cggatagttt taacagttgc gtatttaata gctgacaagg tatcaactgt gttagcgaaa
    28501 ccacagatac cgaatcccat gttagcacgt tggtgtgttg gcaagaaggc catttgaact
    28561 gcttcgtagt tgtatttatc agtcatgtag tggatgatgt taagcgcatc tacataagta
    28621 tctgtcaacc agtcaagaga tttctcaaag ttagccataa cttcgtcgta ctcaaggact
    28681 tctgatgtga ttggttcaac aacgttaaac actttgtagt ctctgtgaac atcatcgtaa
    28741 ccaccgttaa gaccagtaag aagggctttg agaacgttta cacgagcacc gaagtattgg
    28801 atgttatgac gttgttcttc attttctggg tcaagtggtg atacacaaca tgaaatacat
    28861 gacatttcac cataaccttc tttagccatt gttgtcacac cttcatattg gatagaagag
    28921 tgtttgtggc tcattttcat acagtagcga cggaatgttt ctggcagttg gtcagtccaa
    28981 agaactgtta agtttggttc tggagagtta ccgatattat caagagtgtt taagaaacgg
    29041 tagtccatct tagtcacacg gtgacgaccg tcgttaccca taccagccat agaagttgtg
    29101 atgaatgttg ggtcacctga gtaaagggca tcgtaagctt tcgtacgacc aaattttacg
    29161 gtacggagtt taaggacaaa atcatccaca aattcttgga tttctgattc tgtaaaggta
    29221 ccgcgagcaa ggtcacgttc tgcaaagata tcaagaacga ttggcacacg tccaagagaa
    29281 gtagcagcac cgttgatcac acgacataca gccatgaaag cgatgtttac ccattggata
    29341 gcttctttaa cgttttgtgc tgggcgacga acatcaactc cgtaaaggtc accaagttta
    29401 accacttcac caagagcttg gtattgaagg ttaacttctt cgcgaagacg aatggattct
    29461 tcatcgattt cagtgatagc attccagtca ttgacttttt cttgcatcaa gtagtcagca
    29521 ccgtaaagag caagacgagc gtaaacaccg atgatacgtc cacgtgagta agcatctgga
    29581 agaccagtta cagtgtgggc atgacgagcg cgacgaatat ttgaagtata agcgcggaag
    29641 ataccgtcgt taacagttgt cacatactta gtaaagattt catgtactgc tgggtctggt
    29701 tcatagccgt tttctttaag ggtcgtttcc gccatacgga taccaccttt tggcatgaag
    29761 ttcaatttga acaattcatc gttttgaata ccataaatta attcattttc tttatcgatg
    29821 aaaccagcag gaatgtcagc aattgaagtt ggacgagtat cgtatgggaa acgtgtagct
    29881 tcatagtgtg cttttgtttc ttcgataact tttttgatgt gaagtgaacg ttcagtagca
    29941 ccagccaaga aagattcatc gccatcgtat ggggtgtagt tagcttgtac aaaacgtgag
    30001 acacttgctt tttctttcca gtcagttcct ttaaagcctt cccaagcttt ttcgaaaaca
    30061 tctgtattgg ttttaacagt tgccataaat ttctccttaa attctagtaa caaagcgatc
    30121 tgttacattt acaattctaa gtttaacaca taggaaacgt tttcccaagc aaaattcttg
    30181 acaaaattaa gattctttta taatacagat tgttgaaaca gtcacaaact gtattagaat
    30241 gtattttttt ggctttaggt agcctaaaag gtataataga gaaaggaggg acttatgctt
    30301 atttttccac tgattaatga cacgtcacga aaaatcatcc atattgatat ggatgccttt
    30361 tttgctgcag ttgaggaaag ggataaccct gctttaaaag gaaagcctgt tgtgattggg
    30421 aaagatccaa gagaaacagg tggtcgcgga gttgtttcca cttgtaatta cgaagcgaga
    30481 aaatatggca ttcattcggc catgagctct aaggaagctt atgagcgttg tcccaaagcc
    30541 atttttattt caggaaatta tgaaaagtat cgaacagttg gggaccagat ccgccgtatt
    30601 tttaagcgtt atactgattt ggttgaacct atgtctattg acgaggctta ccttgatgtg
    30661 actgataata agttggggat taagtcagcc gtcaaaatag ccaagctgat tcagcatgat
    30721 atctggaaag aagtaggatt gacctgttca gcaggtgtgt cttataacaa atttttggct
    30781 aaattggcta gtgattttga aaaaccccat ggcctcaccc tagtcttgaa agaagatgcc
    30841 ctgtgctttt tagccaaact ccccattgaa aagtttcatg gtgttggtaa aaaatcagtt
    30901 gaaaaactgc atgacatggg gatttataca ggacaggatt tgttggcagt tcctgaaatg
    30961 accttgattg atcattttgg tcggtttggt tttgaccttt accgtaaagt gagaggcatc
    31021 agcaattctc ctgtcaagtc tgatcggata cgcaagtcaa ttggcagtga gagaacctac
    31081 gctaaactgc tttatcaaga aacagacatc aaggcagaga tcagtaaaaa tgctaagcgc
    31141 gtggccgctc tcttacaaga ccataaaaag ttaggcaaga ccattgtgct caaagtgcgt
    31201 tatgctgatt ttaccacctt gacaaaacgt gtcaccttgc cagaattaac cagaaatgcc
    31261 gcacaaattg agcaagtagc tggggatatt tttgacagct taagcgaaaa tcctgctggt
    31321 atccgcctgt taggggtaac catgaccaat ttggaggata aggtagctga tatttccttg
    31381 gacctatagg agaaagaggg ctctatccgt ccgcctcatt ttggagaaaa aagaagctga
    31441 ttagggattt ccctaatcag cttctttggt ccattatttt attacagcat agcttggtct
    31501 gtatcatcac ctttttcgtt aagggtttta gccttgtaaa aagcaaagcc ataaagagca
    31561 gcttgtataa ggataaggat agttccaacc attgacaagg ttgttgtgaa ctggttaacc
    31621 aagccgataa cagaaaggat aagtcctaag atataaggaa tataactaac cgttaatcct
    31681 tgttttagtt tggataagtt ttgagcacaa aagacaatga tagcaataat agcaaggaca
    31741 tttaagacag ataagaaaat cataaatggt gtcatgctgc tttgtagctg tgccaattgt
    31801 tcagttgtta attgggcccg aaatgcttca ttcttgagtt ggctttgtaa gtagaaaatc
    31861 cctgcaaatc ccattaatga cagtacaact ccaagcccgt ttagaataat aatggcaata
    31921 gtagacgttt tgagtgcctg acgcactttt tcataagaaa tcatataatc ctcctagagt
    31981 ccttaatccg aaattgtaca atgtgcttaa tacaaaaata ttataccata gattaatcaa
    32041 aaatgtccgt ttttctagga ttttttctgg aaaaagaggg caatatctga ctctgtaagc
    32101 cctatcatcg aatcgatagt cgaataattc tcaggtgtta acctaaaatc atcattatct
    32161 gactgagagt tctcagtggg gttagagttt gttgattgag aggaagattt ttgagagtcg
    32221 tcctcaaggg ttgccgtatt agaaaaaagg gaggtctgct cttttgattt tagctcttgg
    32281 ttaggttgag aggaggctaa gtcggattgt tcttggaagc gttcgatcaa gtaggtttgg
    32341 cgtttatcgc cttcgtgttt aatagcatac tcaaaggcag tatattctcc caacagaatt
    32401 aacttacttt tagaccgagt aatggccgtg taaatcacat ttcgttgcaa gagtcggccg
    32461 ctttggcgtg tgataggtaa aatcaccgct tgaaactcac tcccttgcga tttatgaatg
    32521 ctcatggcat aggccagggt taattttagc cattcatttc tagggtacgt gacttcgcta
    32581 ccgtcaaaat ctaagattaa ttcgtcttgc ttagattcgg tgtatttagc aggaatcaaa
    32641 tctgtaatat aaccaatatc tccgttaaag acattcaact gagcatcgtt gactaagtgc
    32701 aggactttat cacctttacg aaaatacgta tcattgaaca gaaattctgt ttgtccttgc
    32761 aagggattga gaagttcttg catgagttga ttaagatgat tgatgccagc ttgtcctttg
    32821 tacataggcg ctaaaatttg aatttcctca gcagggatgc cacttttaat agcagatagg
    32881 acaatttttt gaatcatgtc agggataaag gctgcttggg cttcaaaata agaacggtca
    32941 gcttttttat cacgaaagtc agcagctaag attcctcgac gcatctgatc tgccaaattg
    33001 acaatagtag attcttggga ttgacgaaat atcttttgca aggcgatttg gggcaggcta
    33061 ttgactttta aaagatctga caggacttga ccgggtccaa ccgaaggaag ctggtcacta
    33121 tctccgacaa taatcacttg agttgttgag ttaatagctc ctaacaactg gttggcaagc
    33181 caggtatcta ccattgaaaa ttcatcgaca atcagcaagt cgcagtctag ataatcctcc
    33241 atggcttggt aatcgttgtc gccattgagg cctaagtgcc tgtggatggt tgcgctagga
    33301 agtccagtca actcattcat gcgacgtgca gctcgacctg ttggagctgc taacaagata
    33361 ggaaggtctt ttttatccaa atcaatctga tgcaggttcg cgtaagcctg caaaatgcct
    33421 cgaataacag ttgtttttcc tgttccggga ccacctgtta ggagaaagac cttgctggtt
    33481 aaggccttag taatggcttc ttgctgcact tggtcatagg tgatggcaaa gtctttttga
    33541 acggcttgga tggttgtttg aatggtatca tgactaaatg actgacttaa aggggtatct
    33601 aagagacgag agatgttgtt agcaatccct tcttcagcaa aataaaggct agcatcaaac
    33661 agtttggtgt cactgttttt gattttgccc tctatgatca actcgcttag ttgttcagcc
    33721 acagcagcag gatcacattc tacttgacgt gcatcttcaa ggagtgtgat ggcaaagtct
    33781 aataattgtc gtgcttgaac ataagtatct cctcgattga tggattcttc caagagacaa
    33841 tgaaggaggg ctgcacgaaa acgtttcgga gagtcgcttt caatccctaa gttttcagcg
    33901 agagcatctg ctattttaaa cccaaaacct tgtagatctt cgactaactg atagggattt
    33961 tctttaacaa ggtcaagagc ctcttcttta tacttttcaa acgcttgaaa ggcaaaacgg
    34021 ttgctaaggc caagttcgac gagaccagca atcagttgct ctgtgccgta attcaatttt
    34081 agtttagcga caaaggcttg acgattagcc ttagataggc cagagatagt ttctaattta
    34141 cttgggtctt ctaaaatatg gtcaatagta ttatgaccat atagtgcaat gattttctcc
    34201 gctgtttttt taccaatccc cttaaaatgg tcactagaga aataattaac caaaccagat
    34261 gaactaggtt tgattttttg gtaacggctt agcttgagtt gttgaccgta tttagggtgc
    34321 tgggtcaatt ctccccaaaa ggtgtagtca tctccttcaa taatgtcagc catcgttcct
    34381 gtgataatga tttcaaagtc gtcaatgtca ctgtctgtat cctcaatggc aaggaggaga
    34441 attttaaaga aattagcctg gttttcaaaa atgatacgat caacagtacc tgtaaaaacg
    34501 tattccatag taactctttc tatttataca agataactga aaacctgccc aaaggcaggt
    34561 tttttctaat taaagacggt cattttattg agtggccaaa aacgagcttt cacttcaccg
    34621 ataaggtttt cttttttgaa actaccaact tcgcgactat cccgggaaac aatacgatca
    34681 tcaccaagaa gaaggtattc tccttttgga acactcatct caaagcgtgt ttgtccctca
    34741 gaattagttg taaaagcatc agatgtttct gctaactgtt ggaatagggt attataggcg
    34801 taagtttttt ggagtttatc gtttttaaat tgttttagat actcagccaa atacggctca
    34861 actgtttttt taccattaat ataaagtgtg tcatcattat aagaaatggt atcgcctggc
    34921 aatccaataa ctctttttac gatttctttc ttttgtccgt tttcttcttc ctgagcaact
    34981 acaatatcaa agcgatcaat cctagcttga ttaaaaacga taaggcgttc gccatgagct
    35041 agagttgggt ccatagaatg gccgtctact ttaacagcct gccacaaaaa aagacgagat
    35101 agaccaaaga gaattaaaaa gagagtgaat gggccccatt ctttaataaa ctgtttcata
    35161 tgattccttt tatttagtga gaagcgcttg cgctttgtga gtgttggcga agtgaagttt
    35221 agcactatat tctaagctag acataccata agcggctaag agttggctag caactttatc
    35281 tgaagctgaa ccagcaccgc ttgggagttg gtaacctaaa tcttgaccta attgatcgag
    35341 attgtctaaa aatagatttc tagcaatgat agagctaaca gccacagcca aatagtgact
    35401 ttctgccttt tcttgaaagg ttagaggatt cgggaaatgg tttttttcct tttttagatg
    35461 cttttcataa tttgattggc tagtgaaagc atcaataaca atttgtttag gttgaatgcc
    35521 tttttgtaac aacaaaaaaa tagcttggtt atgaagagca actttgatag aaatggcatt
    35581 atatggttta cttttaccaa ccaattcgtt atatttttta ggtgatagga gaagtgattg
    35641 atgaggtatt tgtttctcta acagaggcgc aatctgttga atggttttat ccgataattt
    35701 tttagaatca tcaaccccaa gctttttcag gaagggatga tctttaggat cgacaaaact
    35761 tgccacaaca gcaatcccac caaaataaga gccattaccc acctcatcac tgccgattat
    35821 aggtatatct tggctattgt tgcttgcttc aaaaactgtc ttagctactg gaagattaag
    35881 ctcttgagct aaagcatttg caccgtttcc ctggagaact aattttccgg atttgtacag
    35941 caaaacagta accccatttt ttttagcagc aaaggctaca taggtatttt ggctactgat
    36001 ggtgtagggg gctagctgtt tttttaaatg tttactcagt atagcatcta ttttcaaaac
    36061 taaggtattc ataataatta ttttaacata aaatagttga ctatggttaa ttcataagtt
    36121 tatttaacaa agaaaacaga gactcgaatg tttagacggg ttagggcaat ggtgttgact
    36181 attttacgaa agtttcattt tacggtataa tgctaatgag gtgaccctat gaaaagtatt
    36241 aatcgctata aattcacctt tggtgaaaaa acattaacat tgacaactga caaagacaat
    36301 ctctttatgg aagaagttga gcgggtagct aaagaaaagt atcaggctct taaaaatcat
    36361 ttaccagaag ctgatgacga gacaatcgct attttaatgg ctatcaatac cttgtcaaca
    36421 caattaagca gagagattgc cattgaaaaa atggaagctg aaattttaga tttacgtcaa
    36481 aaaaccttgg ttggtcttca agaaaaagct aatcaaatag atgtgaatga ggtttaatca
    36541 tgttatcttt attgatagtg cttgttttaa cttggaattt ttatattggt tatagtagag
    36601 gtattattct tcaatcgttt tatgtcttag gagccctact ttctcttttg gtagctaatc
    36661 gattttatat tggattagcc cataagctaa cgctttggat tccttattct aacccggtag
    36721 aaggaacatc agtctttttc tttaaatcag ttgatatctt tgttttagat aaggtttatt
    36781 atgcaggcct tgcctttttt atcattttct tattggggta tgcactgagt cgctttcttg
    36841 ggatttttgt tcatttcctt ttgctagatt attttgataa ccagtggaca aaatgtttga
    36901 gtggcggact agcgcttcta gtatcgcttc tttttttgaa catgttactg tctatttttg
    36961 caacggtccc tatgcctttt ttacagcact atttgcactc tagttttctt gctcgtttgg
    37021 tgattgaaca ccttccacca ttgacaataa ttattcaaaa attgtggatt caagccatta
    37081 tttgatatcg ttttgtgact aatataagaa cctggagtga gtttcacctt taggttcttt
    37141 atttacgaga aactgtttag aaataaagag aacgcatggg aacttagtga ctttcttgcc
    37201 acattttcca tcattttagg tataatgaag agtatgaata acaagatttt agagcagtta
    37261 gaatttaaca aagttaagga attgctatta ccttatctca agacagaaca atcccaagaa
    37321 gaattatcag agctggagcc gatgacggag gctcctaaaa tagaaaaaag ttttaatgaa
    37381 atttctgaca tggaacagat ttttgttgaa catcactcat ttggcatagt cagcctaagt
    37441 tcaatctctg agagtttaaa acgcttagag ctttcagctg atcttaatat tcaagaactt
    37501 ttggctatca aaaaagtttt acagagttct tcggatatga ttcactttta ttctgatttg
    37561 gataatattt ctttccaatc tttggatcgt ttgtttgaaa atttggaaca attccctaat
    37621 ctgcaagggt cttttcaagc tatcaatgat ggtggttttt tagaacattt tgcgagtcca
    37681 gagttagagc gtatccgtcg tcaattaaca aatagtgaac gacgggttcg tcagatttta
    37741 caggatatgc ttaaggaaaa agcagagctt ttatcagaaa atctaatcgc tagtcgaagt
    37801 ggacgaagtg tcctaccagt aaaaaatact tatcggaatc gtatttctgg tgtggttcat
    37861 gacatctctt cttcaggaag tactgtttat attgagcctc gtgctgtagt tacactaaac
    37921 gaagagataa cgcagcttag agctgacgaa cgccacgaag aaggtcgtat tttacacgca
    37981 ttttcagact tgttaagacc tcatgtcgcc actattagaa ataatgcatg gattcttggg
    38041 catcttgatt ttgtaagggc taaatatctt tttatgtctg ataataaggc gacgatacct
    38101 aagatttcta atgacagcac gttagcatta atcaatgttc gtcatcctct gttaagtaat
    38161 cctgtggcta atgacttaca ttttgatcac gatttaactg caattgtcat cactggtccc
    38221 aatactggtg gtaagacgat tatgctaaaa acactcggtt tagcacaatt aatgggacag
    38281 tctggtttgc caatattagc ggataaaggt agtaaaattg cagtatttaa caatatcttt
    38341 gcagatattg gcgatgagca atctattgaa caaaatctat caactttttc tagtcatatg
    38401 acgcacatag tcagtatttt aaacgaggct gaccacaata gtttagttct ctttgatgaa
    38461 ctgggagcag gaacggatcc tcaagaaggt gctagtttgg ctatggctat tttagaacac
    38521 cttaggttaa gtcatatcaa aacgatggcg accacgcact atccagaatt aaaagcttat
    38581 gggattgaaa caaattttgt agagaatgcg agcatggaat ttgatgcaga aacgcttagc
    38641 cctacgtatc gctttatgca aggagttcct ggacgctcaa atgcatttga aattgcttct
    38701 cgccttggtt tagctccatt tattgttaaa caagctaagc agatgacaga ttctgattca
    38761 gatgttaacc gtattattga acagttagag gcacagacac ttgagacacg tagaagactg
    38821 gatcatatta aagaagttga acaagaaaac ctcaaattca atcgtgcggt taagaaactc
    38881 tataatgaat tttcacatga acgcgataaa gagttagaaa aaatctatca agaagctcaa
    38941 gaaattgtag atatggcttt gaatgagagt gatactatct taaaaaaact caatgataag
    39001 agtcaattaa aacctcacga aattatagat gctaaggcac aaataaaaaa attagcgcct
    39061 caagttgatt tatcaaaaaa taaagtctta aataaggcta aaaaaatcaa agcagctcgt
    39121 gctcctagaa ttggtgatga tattatagtg actagttatg gacagcgagg taccttaact
    39181 agtcaattaa aagatggccg ttgggaagca caagtgggaa ttatcaaaat gacattaaca
    39241 caagatgaat ttagcctcgt tagagttcaa gaagaacaga aagtcaaaaa taaacagatt
    39301 aacgtggtta aaaaggctga tagttctgga ccaagagctc gacttgatct tagaggtaaa
    39361 agatacgaag aagcaatgca agagttagat cattttattg atcaagcatt gcttaacaat
    39421 atgggacaag ttgatatcat tcatggtatt ggtacaggcg ttattcgtga gggagtgaca
    39481 aaatatcttc gtcgtcataa gcatgttaag cattttgctt atgccccaca aaatgcaggg
    39541 ggatctggtg ccacaattgt aacgttaggg taaatgatgg aatcatatga acacttttat
    39601 gccaaactat ctcaaccttt tagaaaaacc ccacagttaa taattctttt aaatgtttta
    39661 ctaaaaataa tcacaggagt gatgtacata ctttatccat cttttctgat atttacgctt
    39721 tggcaaggga tgacatttca attatggtta aggctattaa ttattcctgc tgttggcttt
    39781 atagctttgt cttacattag gaaacgcttt gattttccac gaccgtatga aaaatggaat
    39841 attaaacctt taattgataa ggatacaaag ggaaggtcaa tgcctagcag acatgttttt
    39901 tcagctacga tgattagtat gtgtttgtta cgatattatg tttactttgg aatagtttgt
    39961 ttaatattat cggttttatt ggcaatttgc cgtgtgatag caggtattca ctatcctaag
    40021 gatgttattg taggttacct tattggcttg atgttagggc tttgtttatt gatttagcga
    40081 gttcgtagca aatagtttca aaaacaacta aattcttgta caataggctt atcattaaga
    40141 tgaaatggag aaaaaagatg gcattagaag taacagatgc tacatttgta gaagaaacta
    40201 aagaaggttt agtactcatt gatttttggg caacttggtg tggtccatgt cgtatgcaaa
    40261 caccaatctt agaacagtta tctcaagaaa tcgatgaaga tgaactcaaa attttgaaaa
    40321 tggatgttga tgaaaatccg gaaactgctc gtcagtttgg tattatgtca attccaacat
    40381 tgatgtttaa aaaagacgga gaagtggtta aacaagttgc aggtgttcat actaaagacc
    40441 aactgaaagc tattattgct gaattgagtt aactaaaata aagcccagcg tcacgctgag
    40501 ctttatttta tgacctacta acttcaatgg aataaccatg taaacataaa acgacttgca
    40561 aaaaagatgg caacagtaat gaggaaaaat acagtgacaa taagggggtt ctgataagga
    40621 agttttgctc tttcttctcg tttttcgatt tcgtctaata attttccact tgaaacagct
    40681 aggatttcct tataaggttg cacatcatat tttttcttaa aacgttccac acgattagca
    40741 taagtcatag taattatcga taataaaaca aaaatgccaa gtcctagcca ctctaagaaa
    40801 tagactaatg gataacttga aatcattaat aatatcatca atgttaccat tactttggta
    40861 tctcgtttaa aattcttttt atcaacctga gtgatggtat atttcatttt ctcaatgtct
    40921 cctttaatga gttggtctaa gctcacttga aaaatttgac ttaggagtaa gaggctatgg
    40981 atgtctgggt aagttttgtc attttcccag ttagatatgc tctgatggct tacatagact
    41041 ttctcggcta actcttcttg cgagagcgct tgttgtttac ggtagcgaat aatttgctga
    41101 ccaatttcca taaatatctc ctttcataat attagtataa gaatgataag ctaacgggtc
    41161 tatcaaatgt atttgacatt agggatagat cactatttat aaggtattaa aagacaatct
    41221 gaggacaact ttacagcggc gtaaaggaat tgtaaacaaa aagtgaaccg tcatagttca
    41281 cttgtgatta agcatttttc tgttttaagt aggtctctag cattttcttt tgtggtgttg
    41341 caaagggata cagagaaaag tcctcaattg ctacccactt taggtgagga gcattaggta
    41401 aatctgttgc tttgaccacc ccctctatga gttctattgt ccacttctgg tgactaaaag
    41461 tgtgcttaat atttggaaag tgattatcag tccattgggg ctttaattga tattctcttt
    41521 cgaaagtttc attttgggtt tgccagatta tggggttgga ttgattgtca tcaaataaat
    41581 ctaattgttg agaaagaggt gacgtttcga taataggaaa tgaccaaaaa cctcctagaa
    41641 gtctcccttt agtatttttc tccagtagat attggccact ttggttacga atgacaaaag
    41701 cctgaattcg cattggttta ggctttttct ttggattttt tatgggatat ttaccataag
    41761 taccattcaa ataagcagcg ttaaagaaac gaatagggct ttcatcaggc ctaggtgtct
    41821 tagcggattc gatgtctgta cctagatcca taagtgcctg gttaaaatca ccgggtctgt
    41881 cagggtcaat tagtatttcc ataatagctt gaaaaatttt acgattttta gggtcaccga
    41941 tatcataatt aacttcaaac aaacgagcca tgacacgcat cacattgcca tcaacagcag
    42001 gctctggtaa gttgaatgag atgctggcga tagcacctgc agtataagga ccaatccctt
    42061 ttaatgaagc aatatcatca taagtgtgag gaaatatgcc accaaaatca accatcactt
    42121 gctgtgcagc cttttgcata ttacgcaccc gagaataata gcctagtccc tcccatgcct
    42181 ttagtaattg ttcttcagga gcgtctgcca aatcttttat ttgaggaaac cactctagaa
    42241 aatgtttata ataagggatt accgtattaa cttgtgtttg ttgcaacatg atttcagaaa
    42301 cccaaatata gtatggatta gtagttcttc gccaagggag atcacgtttt tcttggtcat
    42361 accaccctaa aagtgtgcga cgaaaagaag cgattgtctc attatcccac atattaatac
    42421 cataatcttt taattcaatc atgttgttat tgtaacacag gagccttaaa aagagtaaaa
    42481 aaagatttta ggtatttgcg aaaaagaaaa ggaaatatta taataatatt aggtttacct
    42541 catctaaatg ttgatttaga ataactgaaa ttaacgatag aagcagacag gttttaattt
    42601 ggaggggaca tgttccagaa attaaagaat cttattgatc ggtattcgtg gttcgttttt
    42661 ataatgatga ttcttgtgtt atttcttaac tatatttatc catttgatcc ttctttgagc
    42721 ggactacagt tggaatggct gatcatgacg caagtttttc ctggattagg tatcgtatgt
    42781 gccatggttg ctattatttt taaaaagttt aacctttttg ttttggccct aattttatat
    42841 tttgcacttt ggttaaaact tttccttgct ttttcttttt tcacctattt tttaggagtc
    42901 tgactaagtg ccaattttta aaactcgtga aagaaaatgt ttgacaaaac ttagtcaagt
    42961 agaaaaagtt ccttgctttt aattgactat tagtgtacaa taagattctg tgagtatacc
    43021 tcacttactc actccaaaag tagtgagtca ttagaccaaa aggaggaaaa tatcaatggc
    43081 taaatacgaa attctttata tcattcgtcc aaacattgaa gaagaagcta aaaacgcttt
    43141 ggtagcacgc tttgactcta tcttgactga caacggtgca aatgttgttg aatcaaaaga
    43201 ttgggaaaaa cgtcgtcttg catacgaaat caacgatttc cgtgaaggac tttaccacat
    43261 cgttaacctt gaagcgactg acgcagcagc tcttaacgag tttgaccgtc tttcaaaaat
    43321 caatggtgac attcttcgtc acatgatcgt taaacttgac gcttaattcc aactaagagg
    43381 gtagtttatg attaataatg tagtactagt tggtcgtatg accaaggatg cagaacttcg
    43441 ttacacacca agtcaagtag ctgtggctac cttcacactt gctgttaacc gtacctttaa
    43501 aagccaaaat ggtgaacgcg aggcagattt cattaactgt gtgatctggc gtcaaccggc
    43561 tgaaaattta gcgaactggg ctaaaaaagg tgccttgatc ggagttacgg gtcgtattca
    43621 gacacgtaac tacgaaaacc aacaaggaca acgtgtctat gtaacagaag ttgttgcaga
    43681 taatttccaa atgttggaaa gtcgtgctac acgtgaaggt ggctcaactg gctcatttaa
    43741 tggtggtttt aacaataaca cttcatcatc aaacagttat tcagcgcctg cacaacaaac
    43801 gcctaacttt ggaagagatg atagcccatt tgggaactca aacccgatgg atatctcaga
    43861 tgacgatctt ccattctagt cagaatggat tgaacgaata cgaaattatt tgagttcggg
    43921 ctaaaaattt agaacttaga atttagatca actccctagg aaatcaagat ttcttgccag
    43981 ttttctaaaa ttcagtcgtt ttttaagagc ccttaacatc ataatttata aaggagaaaa
    44041 tacatggctc aacaacgtcg tggcggattc aaacgccgta aaaaagttga cttcatcgca
    44101 gctaacaaaa ttgaatatgt tgattacaaa gatactgagc ttcttagccg tttcgtttca
    44161 gaacgtggaa aaattcttcc tcgtcgtgta acaggaactt cagctaaaaa ccaacgtaaa
    44221 gtaacaacag caatcaaacg tgctcgcgtt atggctctta tgccttacgt aaacgaagac
    44281 taataaacaa acagtccatt tcggactgtt ttttagttga ctaggactag tctaggcgta
    44341 taggtgagtt ttttctaaaa gagagaatga tagtaaacga aaatccttgc gtagacagca
    44401 aggatgaaat gattgttatt tgttaacagg tgacatagtg ttttgaatgt tgtatttgcg
    44461 ttgtaggtaa tagcgtaaag caagggaaac accaccaata atgagcaagg caagaggagg
    44521 caactgtggg ttaagagagg ttggtaaaaa ggcagttgca gagtacaagg caatccaaat
    44581 caacatggct aatgataagg ctgcgatgat tttgaaccaa cttggacgaa ggctcttatc
    44641 ttttccgaga tgacggtaaa taaagtaata agtagcgtac atggatgctc ctccaccaaa
    44701 accaagtgcc agtaaggaaa tcaatcctgt aactgtagcg tttgtgttga agaaggtcat
    44761 gatgccattt aaaagggcaa caattccgat gaatagcaaa gaggtatcta gccacatcaa
    44821 ccaaggattg gtgtttttgt cagtttctgc tgctctttga ctagggtctt ggctaaaaga
    44881 agctgcccaa gcagtcggag tgcctaatag atttcgagcg gtgacacctt ttttctggtt
    44941 ttctagaata gctggaatgg cttcttcgag aatcagctta atgtcttcat cagacttgcc
    45001 gtcttggatt aatttgttcg tagcggtgtg gataaattct tgattttttt tggtaagttc
    45061 ttgtaattcc atgggaatcc cttctttata tgggttagtt aaagtaagtc aatggtatta
    45121 gctagttgta atgaaacttt ggagctagcc gtttgacaca gagttttgtc aggtcttaac
    45181 cgcattctgc agcttgctcc actctagaac catttttttc taataaagta aatcacaaca
    45241 aaggaactaa gtagcatagc aatcaaggta atataccaaa aagcatgttc taggccattt
    45301 agtggtaacc agttgttttg gaaattcata ccataggcgg agaaaatcac agttggtata
    45361 tctaaagcca tggtcatcaa ggccagtgtt ttcataatgg tgttctggtt attgttgata
    45421 atggaagcag tggtttcggt catggcattc aagacatttt cataaatgcc agccatctca
    45481 atcgcctgtt gggtttcaat caaggtatct tctagcaaat cctcgtcctc aatgtatttc
    45541 ttgagagagc ttgttgagct ggataatttc ttgacgatgc gttcgttgaa tttcaaggaa
    45601 gcctttaagt agacaatgga tttttcaagt tccatcatgt cgataagttc ttcatttcga
    45661 gtagctgctt caagctgagc ttccaaacgt tcgctttggc gatcaatggt cctcaaggca
    45721 gtcagaaaga gctcggcgtt gcgatataga atctgaaaaa caaaacgtgt cttcatgaag
    45781 gtatagaaat ttttgacccg gcggttgtga aaatgatcga aaagggtcat gtcatgcaga
    45841 caagttgtga taacagcatt ttccgttaca ataatgccta aaggcatagt aatatagtaa
    45901 cttttattgt tacgttcttc gtatatcgga acgtctacga taattagggt ataatcatct
    45961 tctacggcaa tacgtgaggt ttcttccacg tccaaaggcg cgcgcaagtc agaaatatca
    46021 atgttaaatt gatcggcgat cttcatagac tcttcttggg aagggttaac cagtttaatc
    46081 caagctcctg gctcgaatgt ttctatttct ttgaattcaa ttgccgatga aagaaacatt
    46141 tgtttcatga caagccctcc tttttgatga ttgactggta gagattgaca tcaaaccttg
    46201 ataataaagc cttctcaagg attttctgaa aaattctcaa tacttatcta ttataccaaa
    46261 attcgcaaag tttggggcaa taggttatca gaaatttttg ttataatata agaaagctcg
    46321 atgaggaggg tgcctttagg cacattcaag atgcaaaata aaataataat tcatggggca
    46381 agagcccaca atttaaaaaa tattgacgtg gaaattccac gagacaaatt agttgtcgtg
    46441 acagggttat cagggtcagg aaaatctagt ctagcctttg atactattta tgcggaaggg
    46501 caacgtcgtt acgtagaaag tttatcagct tatgctagac agttcttggg taatatggaa
    46561 aaaccagatg tggattccat tgacggtctt agccctgcta tttccattga tcaaaaaact
    46621 accagtaaga acccccgctc aacggttggt acggttacgg aaatcaatga ttatttacgt
    46681 ttactatatg cgcgtgtggg cacaccttac tgtattaatg ggcatggggc cattacagca
    46741 tcttctgcag aacaaattgt agagcaggtc ttagccctcc ctgaacggac acgcatgcag
    46801 attttagcgc ctattgttcg ccggaaaaaa ggtcaacaca aaaccatttt tgagaaaatt
    46861 caaaaagatg gttatgtccg tgtgcgggtt gatggggata tttttgacgt gacagaagtg
    46921 ccggagctgt caaaaagtaa gatgcacaac atcgaggtgg ttattgatcg tctggttaat
    46981 aaggatggta ttcgtagccg tttatttgat tcggttgaag cagctttaag gctgggagat
    47041 ggctacctta tgattgacac tatggatggc aatgaactgc tcttttctga gcattattcc
    47101 tgtcctgtct gtgggtttac ggttcctgaa ttagaaccaa gactcttctc ttttaatgcg
    47161 ccgttcggct cttgtcccac ctgtgatggg ctaggcatta agttggaagt ggatttggac
    47221 ttggttgttc ctgacccaag taagtctttg aaagagggag ctttggcgcc ttggaatcct
    47281 atttcctcca attattaccc aaccatgctt gagcaagcta tggcaagttt tggggtggat
    47341 atggatacac cttttgaggc cttgactgag gaagaaaggg atttagtgct ttatggttca
    47401 ggtgatagag aatttcattt tcactatgtg aatgattttg gtggtgagcg taacattgac
    47461 attccttttg aaggtgttgt gactaatgtt aatcgccgtt atcacgaaac taatagtgat
    47521 tatacgcgca atgtgatgcg tggctacatg aatgaattga cttgtgcgac ttgtcatggt
    47581 taccgtttga atgaccaagc cttgtgtgtg catgtgggtg gcgaagaagg acctcatatt
    47641 ggtcaaattt cagaattatc gattgccgac cacttgcagt tgttggaaga actggaactc
    47701 acagaaaatg aaagtaccat tgctaaaccg attgtcaaag aaattcatga ccgcctgacc
    47761 ttcttaaata atgtgggact gaattatctg accttatcac gtgctgctgg gactttatca
    47821 ggtggggaaa gtcaacgaat caggttagcg acacaaattg gttctaactt atcaggggtg
    47881 ctctatatct tggatgagcc ttctattgga ttgcaccaaa gagacaatga ccgtttgatc
    47941 gaaagcctga aaaaaatgcg tgatttgggt aataccttaa ttgtggtaga acacgatgaa
    48001 gacaccatga tgcaggcaga ctggttgatt gatgtggggc ctggggcagg tgagttcggt
    48061 ggagaaatta ttgcctctgg aacgccgaaa caagtggcta agaataaaaa atccatcacg
    48121 ggtcaatatt tgtctggtaa aaaattcatt ccagtgccat tagagcgtcg ttctggtaat
    48181 ggccgattca ttgaaataaa aggagcagcg cagaataatc ttcaaagcct cgacgtgaga
    48241 ttcccactgg gtaaatttat tgcggtcaca ggggtatcag gctcagggaa atcaaccttg
    48301 gttaatagta ttttgaaaaa ggctgtcgcc caaaaattaa accgtaatgc tgataagcct
    48361 ggtaaatatc actctatttc tggtattgaa catattgaac gcttgattga cattgaccaa
    48421 agtccgattg gaaggacgcc acggtcaaat ccagccacct atacgggtgt ctttgatgat
    48481 attcgagact tgtttgccca aaccaacgaa gctaagattc gtggttacaa aaaaggccgt
    48541 ttctctttta atgtcaaagg tgggcgttgt gaggcttgtt caggtgatgg gattatcaaa
    48601 atcgaaatgc actttttgcc agatgtttat gtgccttgtg aggtttgcca cggcagacgg
    48661 tataattctg agacgcttga ggtgcattat aaagaaaaaa atattgctga agtgcttgac
    48721 atgactgtgg atgatgcctt ggtatttttc tctgctattc ctaaaatcgc tcgaaaaatt
    48781 caaacgatta aggatgttgg acttggttat gtgaccttag gtcaaccagc tactactcta
    48841 tctggaggag aagcgcagcg gatgaaatta gctagtgaat tgcacaaacg ctcgacaggt
    48901 aagagcttgt atatcctaga tgagccaacg actggtttac acacagatga tattgcaagg
    48961 ctattaaaag ttttggaacg ctttgtagac gatggtaata cggttcttgt gattgagcat
    49021 aatttggacg ttatcaaatc tgctgaccat attattgact taggaccaga aggtggtgtc
    49081 ggaggtggtc agattgttgc cacggggaca ccagaagagg tagctcaagt taaagaaagc
    49141 tatactggtc attatttgaa agtaaaacta caacaatagg attaggagtt cgtttgagct
    49201 ccttttaatc ttttaaatgc tctcaaatca tggtaaactt aaagtagttt tttcatgcta
    49261 caatattctc aaaagttacc aaaggagttc gcgatgtcag gatttttaga gcaacgatta
    49321 ggtcactgcc taaggcagat ggcagagaag gggctagagg ctcttctggt cacccattta
    49381 accaatagtt attacttgac aggtttttct ggaactgcag caactgtttt gataacggcc
    49441 aaacgtcgtg ttttgatcac agattcacgt tataccttgc ttgctaaagc tagtgttgag
    49501 ggatttgata ttatcgaaag ccgcacgccc ctgaatgttg tggcagaatt gttaggggct
    49561 gatcaaatag attgccttgg ttttgaggac caggtatcgt tttcttttta tcaggccatg
    49621 caagcagaac tgtcaggaat aaccttgctt gctcagtcag gctttgtgga gcatttacgt
    49681 cttattaagg acgtctctga aattgagacc attgctaaag cgtgctcgat ctcagacaaa
    49741 gcatttgaag atgctcttga ttttattaaa ccagggacaa ccactgaacg tgacctggct
    49801 aattttttag attttcgcat gcgtcagtat ggtgccagcg gcacatcatt tgatatcatt
    49861 gcagcttcag gctatcgctc tgccatgcct catggacgcg ccagtgacaa ggttatccag
    49921 aataaagaga gcttgaccat ggactttggg tgttactaca atcactatgt tagtgatatg
    49981 acgaggacca ttcacattgg ccaagttact gatgaagaac gtgagattta tgctcttgtt
    50041 cttgctgcta ataaggctct aattgctaaa gctagcgctg gcatgactta tagtgacttt
    50101 gacggtattc cgcgccaact tatcactgag gcgggttatg gcagtcactt cacacatggc
    50161 attggtcatg gtatcgggct tgacatccat gagaatccat ttttggggaa atctgagcaa
    50221 cttctccaag ctggaatggt ggtcacagac gagccaggta tctatttgga taacaaatat
    50281 ggtgtccgta ttgaagatga cttggttatc acaaaaactg gttgtcaagt cttgaccttg
    50341 gcacccaaag aattaattgt attgtaaaag aatggttcag gaaaggatta ggggagaagc
    50401 aatgacaaat cgtttatcat ggcaagacta ttttatggcc aatgcagagc tgatttccaa
    50461 gcgctctacc tgtgatcgcg cctttgtggg agctgttttg gtaaaggata accgcattat
    50521 tgccacaggt tacaatggcg gagtttctgc tacggataac tgcaacgaag cgggtcatta
    50581 tatggaagac ggtcattgta ttcgcactgt tcatgctgaa atgaatgctt tgattcaatg
    50641 tgcgaaagaa ggaatttcaa cggacggaac tgaaatttac gtgactcatt ttccttgtat
    50701 taactgcact aaggcgcttt tgcaggcggg gattacaaag attacctata aagcacatta
    50761 tcgtccgcac ccctttgcca ttgaactgat ggagaaaaaa ggggttgcct atgtgcaaca
    50821 tgatgtgcct caaattgttt taggaggcga taaggaacaa tagaaagtgt acctaaaagg
    50881 acggtcataa aagatttgag ccaaggccac ttttgtggta aaatgaagtg ataaatattt
    50941 tagtaagagg taataataat gattgaagca agtaagctta aagcaggtat gacatttgaa
    51001 gcagaaggaa aattaatccg tgtccttgaa gctagccacc acaaaccagg taaaggaaac
    51061 actatcatgc gtatgaaact acgtgatgtg cgtacaggtt ctacttttga cacaacttac
    51121 cgcccagatg aaaaatttga gcaagccatc attgaaactg tcccagcaca atacctatac
    51181 aaaatggatg acactgctta cttcatgaac actgatactt atgatcagta cgaaattcca
    51241 gttgctaacg ttgagcaaga attgctttac attcttgaaa actcagacgt gaaaatccaa
    51301 ttttatggaa gtgaagtgat tggggtaacg gttccaacaa ctgttgaatt gaccgttgcg
    51361 gaaacacaac catctattaa aggagcgaca gtgacgggtt cagggaaacc tgcaactctt
    51421 gagacaggac ttgttgttaa cgttccagac tttatcgaag ctggccaaaa actaatcatt
    51481 aacactgcag aaggtactta cgtttctcgt gcttaatcct ttgtcaaaac agattagtct
    51541 aaaaaagaga aaggaaatgc tatgacaact gaatatatcg gtgaaatcgt catttcacca
    51601 cgagtgcttg aggttattac ggggattgct acgacacaag ttgagggcgt ccactcactc
    51661 cataacaaga aaatggcaga tagctttaac aaagcaagtc ttggtaaagg ggtttatctc
    51721 cagacagaag aagatggaag tgttacagca gatatttatg tttacttgca atatggagtt
    51781 aaggtcccga ctgtatctat gaatattcaa aaaacagtca aatctgctgt gtacgatatg
    51841 gcagaagtgc ctatttctgc agttaatatc catgttgaag gtattgttgc tgaaaagaca
    51901 ccaaaaccag atttgaagtc actttttgat gaggatttct tggatgacta acagctttca
    51961 aaattcaaga agagatttgc gtgaacgtgc ctttcaggct ttatttaaca tagagatggg
    52021 agctgagtta ttggcagctt ctcagtttgc ctatggttat gataaagtaa caggggagga
    52081 cgctcaagta ttggagctgc cgattttttt attaagtctt gtgacgggtg tcaataatca
    52141 taaagaagag ttggataact tgatatcaac tcatttgaaa aaaggttggt cgttagaacg
    52201 cttgacacta acagacaaaa cgttattacg actaggtctt tttgaaatca agtattttga
    52261 cgaaacacca gaccgtgttg cactaaacga aatcattgaa gtagctaaaa aatattctga
    52321 tgaaacctct gctaaattta ttaatggctt acttagccaa tatgtctcgg aagctccttc
    52381 agcaaataaa tcataataag tcggagcaat cttaggattg tatccgattt tttcaattta
    52441 tgtacgaata ttaaaagaca aacaagcata ctctcgcatc tagaaagagt atgcttgttt
    52501 gtcttttaga gtaattagtg attggttatg ttttgtttaa atacttgctc ctggaagtag
    52561 cgtaatcggt aaaatatagt ctttactggt tttttctttt ttaatttttt tgatgagtat
    52621 ttcgacactc aatttagcaa tctcatcaat aggttgacga atggtcgcta attgaggaac
    52681 atattgctgg ataaatgtcg ttccatcata acctatgact ttcatatcct ctggaatagt
    52741 aatgtgaagt tgtttggcta ctttcataat aaggatagca gttaaatcat cagaaacgaa
    52801 aaggccatca ggttttcggg ttgccaaaat ggatttaatt tccatttcgc gtctaacagg
    52861 agataggtta tttggtaatt tgaggatttc tgcacttcgt ttgagctggt agttaaaccc
    52921 tagctggcgc aaccctgtgg gcgaatcaga attgtcatta cctgtaatca tgacaatgtt
    52981 ttgacaaccg tgtttttgca aagtttgtgc ggctaattta ccaccttcaa aattatctga
    53041 agaaataacc gggatatttg gcgctagatt acggtcaaaa gctacaatag gagcttcgac
    53101 acgctcataa tcctcaattc ccaaattatg actggaagaa ataatgccgt ccacttgatt
    53161 ggccgcaagc atttctaagt attcgcgttc tttgacggga ttatgttcac tattgcaaat
    53221 aatagttttg tagccctgtt taaacaattc aatttccaaa tgttcaatta attcagcgta
    53281 aaagatattg ctaatgttgg ggaagataag cccaatgagt tgggttgatt taccttgcaa
    53341 acttctagcg agattgttag gcttgtagcc caattcacgc atggccttat tcaccttatt
    53401 cactgttttt tgggaaaggt agcctttttt gttgattact cgcgaaacag ttgttggact
    53461 gacacccgcc aaggctgcca cgtctgttaa ttttgcgacc acttgcctaa tacctcattt
    53521 cataataatg tccaaaagct tgtccttcta ctacttggat acctgtttgt ccttgggttg
    53581 ggaaaacacg actcgttagg actttttgcc cttggttaat aaagatttca agaatggaat
    53641 tgtccacata gacattaagt gtaacatggc cttgtgggat ttggcaagag cgcatcgtac
    53701 catagtcttg ggcatattga acccctgccc gacttcggtc aatgcttaac tgtccttttg
    53761 ttgtatccac cttcaatctt aggccattcc ctttttggtc ggcaaatagg agcaggtcaa
    53821 gctttttttg gcctggaatc gtcaattcca actcataaca gttagaactg tctattttat
    53881 tatgaaataa ttctgctggc cctcgaagat tttgtagggc tgaaacaggt gtttgataga
    53941 gaattccgtc tttaatcctt aattctttca ctaaactaag agctccttga tagtcataac
    54001 gatctgtcgg ataatcaata tctggtaacc caatccaaga cacggctaga accctgccat
    54061 ctggactact aaaggcttga gtggcataag cttcaaaacc aaagtcaaga ttttgaaggg
    54121 cccttccacc cagcaattgg ccagtttctg gattgaagct ttcaaaaatt ttataggtat
    54181 tggggtagat attttgataa tctaaatcag ctttagccag tccttgtggg ctgaaaatca
    54241 aaacaggctt gtcatcgaca aacacaaggt tggggcattc aatcatgtat tccgtaccag
    54301 aatcgtcaag gtccaaatcg gctataaatc gccagttgtc cacatgattg tccacagctt
    54361 tgtaaagttt gatttttcct ttgccatcta aaccttgggc gccaataata gcatagaatt
    54421 gaccttgata agaaaaaagc tgaggatccc gaaaatgttc tgtaacatca tcgggctgtt
    54481 caatcaaaac ctgtggtatt ttggaaatat ttccttgttt atccatccat gcgccaacct
    54541 gtaatggtgt tctgacccaa ttggcatcgc gaacattacc agtgtaaaag aggaagagct
    54601 tgtcatcaat agcatatgca gaacctgagt aagccccatg actatcgtga gcgtggtctg
    54661 gcagaaggcg gctccttgtt tccgtaaaat ggacgaggtc tgtggatgtc atgtgtaccc
    54721 attgtttcag accatgcgca gcgccataag gccagttttg ataaaaaaga tggtagcgac
    54781 cgttgaaata agaaaaccca ttggggtcat tgagtaatcc tgtcttaggt tcgacatgaa
    54841 attggctgtg ccatggggat tgggccattt tttcagtaat agcttggtag tcttttgagc
    54901 tccactcttt gtaagggcgg tagcgaatag cttgtggtag atccatcaaa atctccttgt
    54961 tttatagtcg tcttcaagaa tatactaaac gttttctgtt taaaaatcaa gaatttaaag
    55021 catataaaat aaaaatatgt taaacgcttg acataaaaaa tattagtgat aaaatgatag
    55081 tcaagaagtg aagcggattc actagcgttg gtcatgcaca ttcttagcca caaagctggc
    55141 agaatggagg agggactaac accaaatgaa aaaggagact tttaggcatg gataatcgtc
    55201 agattgcagc tgaagtgatt gaggctttag gtggtcgaga aaatgtgaga agtgttgccc
    55261 actgtgcaac tcgccttcgc gtgatggttt atgatgaagg aaagattgat aaggaaaaag
    55321 cagaagctat tgacaaggtt aaaggagctt tctttaattc tggtcaatat caaatgattt
    55381 ttggaactgg taccgttaat aacatttatg acgaagtcgt tgctcttggt ttaccaacat
    55441 catcaaccag tgagcaaaag gcagaagcag gcaaacaagg caatatcttc caacgggcga
    55501 ttcgtacgtt tggagatgtc tttgttccca ttattccagc tattgtagca acgggtctct
    55561 ttatgggggt tcgtggtttg gtgacccagc cagctattat ggatttattt ggggtgcatg
    55621 agtacgggga aaattttctc atgtacactc gtattctaac ggatacagcc tttgtctact
    55681 tgccagcttt ggtggcttgg tcagccttta gggtctttgg gggtaatcct attatcggta
    55741 ttgttttagg attgatgctg gtttccaatg agttgcctaa tgcttgggta gttgcctctg
    55801 gtggagatgt taagccgcta accttctttg gatttgttcc tgttgttggt tatcaaggta
    55861 ccgttttacc cgccttcttt gttggtctgg taggagctaa gttggagaaa tggttgcaca
    55921 aaaaggttcc agaagcttta gacttattgg tcacaccgtt tttaacgttt gctattatga
    55981 gtatcttggg actatttgtg attggaccag tcttccattc tcttgaaaac cttgttctgg
    56041 ctgggacaca ggccgtcttg catttgccgt ttggcattgc tggtcttatt gttggtggta
    56101 tccaacagtt gatcgtcgtt actggtattc accatatctt taacttccta gaagcgcagc
    56161 taattgccaa taccggaaaa gatcctttca atgcttacct aacagcagca acagctgctc
    56221 aagctggagc taccttagct gttgctgtta aaaccaaatc aacaaaacta aaaggtctgg
    56281 cctttccatc aaccttgtca gctcttttag ggattactga accagctatt tttggggtca
    56341 acctccgtta tccaaaagtc tttgtttcag gtcttattgg tggtgcctta ggtggttggg
    56401 tcgctgggct ctttggcatt gcaggaactg gttttgggat tacagtctta ccaggaacgc
    56461 tcctttactt gaatggccaa ttattacaat accttgtgac tatgcttgtc ggtcttgggg
    56521 tagcctttgc aattgcttat acttggggtt atcaagatag agaaaccctt ccgttaccag
    56581 ctgtcgaagt tgatcaaacg gctgatcagc cagccttagc agaggaaact ttatatagtc
    56641 ctttgaatgg aacagttgta gatttagcag ctgtttcaga ccctgttttt tcatcaggtg
    56701 ctatgggtca aggcttagca attaagccag aagataatac cctatactca ccagttgatg
    56761 gtaaagttga aattgtcttt gaaacaggtc atgcctatgc tataacgtca agtcaaggag
    56821 cagaagtgtt acttcatatt ggtattgata ccgtgtcgat ggcaggagat ggctttgaat
    56881 ctcttgtagc agtaggtcag gcggttaaaa aaggtgatct tcttggacac tttgatccta
    56941 gcaagattgc agaagctgga ctagatgaca ccaccatgat gattgtgacc aatagcactg
    57001 actatcaaag cgtggacatt cttgctcaag gacatgtttt gattggtgat caagtggcac
    57061 ttatcaaata aggacaaaag agctaactaa gcagctcttt tgtcctgcca tgaatggcag
    57121 gttggcaaaa aaatgagaaa agcctaaaaa ccttaaattt gtgttatttt acctatactt
    57181 tgtaccttgt tttttttaat aaagtgttgt tatacttaag gcgaactata ggaatgcgct
    57241 tacatagatg gtatatcaac tgggaagcca tgacttagta ccaaaaataa ggagtgtcca
    57301 aatggataaa catttgttgg taaaaagaac actagggtgt gtttgtgctg caacgttgat
    57361 gggagctgcc ttagcgaccc accatgattc actcaatact gtaaaagcgg aggagaagac
    57421 tgttcaggtt cagaaagaat tatcttctat cgatagcttg cattatctgt cagagaatag
    57481 caaaaaagaa tttaaagagg agctttcaaa agaaaaagta ccagaaaagg tcaaagagat
    57541 attagcaaaa gctcagcagg cagataaaca agctcaagaa cttaccaaaa tgaaaattcc
    57601 tgagaaaata ccgatgaaac cgttacatgg tcctctctac ggtggttact ttagaacttg
    57661 gcatgacaaa acatcagatc caacagaaaa agacaaagtt aactcgatgg gagagcttcc
    57721 taaagaagta gatctagcct ttattttcca cgattggaca aaagattata gccttttttg
    57781 gaaagaattg gccaccaaac atgtgccaaa gttaaacaag caagggacac gtgtcattcg
    57841 taccattcca tggcgtttcc tagctggggg tgataacagt ggtattgcag aagataccag
    57901 taaataccca aatacaccag agggaaataa agctttagcc aaagctattg ttgatgaata
    57961 tgtttataaa tacaaccttg atggcttaga tgtggatgtt gaacatgata gtattccaaa
    58021 agtgaatggg aaagctagtg acgaaaacct caagcgatct attgatgttt ttgaagaaat
    58081 tgggaaatta attggaccaa aaggtgttga taaatcgcgt ctgtttatta tggatagcac
    58141 ttacatggct gataaaaacc cattgattga gcgaggagct ccttatattg atttgttatt
    58201 ggtacaagtc tatggcgcaa gaggagaaca aggtgagttt caaaacgata ccaagttagt
    58261 taccgagact cctgaagaac gatggcaagg ttatagcaag tatattcgtc ctgaacaata
    58321 catgattggt ttttctttct atgaggaaag agcaggcagc ggcaatcttt ggtatgatat
    58381 taattctcga aaggacgatg acaaagcaaa tggaattaac actgacataa ctggaacgcg
    58441 tgccgaacgg tatgcaaggt ggcaacctaa gacaggtggg gttaagggag gtatcttctc
    58501 ctacgctatt gaccgtgatg gtgtagctca tcaacccaaa caaatagctg aaaaagataa
    58561 acagaatgta aaaaataatc agcctcagat tccagagatt actgataaca ttttccactc
    58621 agattatagt gtctctaaag cattaaagac agttatgcta aaagataagt cgtatgatct
    58681 gattgatgag aaagatttcc cagataaggc tttgcgagaa gctgtgatgg cgcaggttgg
    58741 aaccagaaaa ggtgatttgg aacgtttcaa tggcacattg cgattggata atccagcgat
    58801 tcaaagttta gaaggtttaa ataaatttaa aaaattagct caattagact tgattggctt
    58861 atctcgcatt acaaagcttg accaatctgt tttacccgct aatatgaagc caggcaaaga
    58921 taccttggaa acagttcttg aaacctataa aaaagataac aaagaagaac ctgctactat
    58981 cccaccagta tctttgaagg tttctggttt aactggtctg aaagaattag atttgtcagg
    59041 ttttgaccgt gaaaccttgg ctggtcttga tgccgctact ctaacgtctt tagaaaaagt
    59101 tgatatttct ggcaacaaac ttgatttggc tccaggaaca gaaaatcgac aaatttttga
    59161 tactatgcta tcaactatca gcaatcatgt tggaagcaat gaacaaacag tgaaatttga
    59221 caagcaaaaa ccaactgggc attacccaga tacctatggg aaaactagtc tgcgcttacc
    59281 agtggcagag ggaaacattg atttgcaaag ccagcttttg tttgggactg tgacgaatca
    59341 aggaacccta atcaatagcg aagcagacta taaggcttac caaaatcata aaattgctgg
    59401 acgtagcttt gttgattcaa actatcatta caataacttt aaagtttctt atgagaacta
    59461 taccgttaaa gtaactgatt ccacattggg aaccactact gacaaaacgc tagcaactga
    59521 taaagaagag acctataagg ttgacttctt tagcccagca gataagacaa aagctgttca
    59581 tactgctaaa gtgattgttg gtgacgaaaa aaccatgatg gttaatttgg cagaaggcgc
    59641 aacagttatt ggaggaagtg ctgatagtca agcagcttta aaagcctttg atggggtaat
    59701 agagtacaat ccgatatcta ctcagaatag agcttcgatt atctttgaaa tgaaagaccc
    59761 tagtttagca aaatattggc gccttttcaa tgatagcagt aaaggcgaag acgactatat
    59821 caaagaagca aaacttgaag tctttacagg ccagctgaat gctgaagcag atgtgaaaac
    59881 gagcttggaa aaatcagatg attggcaaac tgtttcaacc tattcaggac aagaacaggt
    59941 atttagccat gcactggata atattagtgc taagtactgg agagtaaccg tagatactaa
    60001 gggaggaaat tatagttggc catcactccc tgaactccaa attttaggtt atccgttacc
    60061 taacgccgac actatcatga aaacagtaac tactgctaaa gagttgtctc aacaaaaaga
    60121 taagttttct caaaagatac ttgatgagtt aaaaataaaa gagatggctt tagaaacttc
    60181 tttgaacagt aagatttttg atgtaactgc tattgatgct aatgctggag ttttgaaaga
    60241 ttgtattgag aaaaggcagc tgctaaaaaa ataaacaaag taactctctt agatagcaac
    60301 attcagatta aattaacaaa atgtgactat gataaaggtt tgctggaatt gattaaccaa
    60361 aagactaaaa atctgagatg aatagtccca gatttttagt cttttatagg ttttgatgac
    60421 ataaagctaa ataatcgtta gactaccaga aagtggcgct tgtccgtgag acatggctgt
    60481 cttgataaga gcctgtgtca tcataaagat aaaaaagaac aagaaaaagg agaaaacatg
    60541 ggaaaattat atggaagtat tgaagctggt gggacaaagt ttgtctgtgc cgtgggtgat
    60601 gaggagttta cagtggtgga taaaacacaa tttccgacca ccacaccaga agaaaccatc
    60661 gcgcaaacca tcgcatattt taaaaccttt gaggccgatt tggctggaat ggcgattggt
    60721 tcttttggac caattgatat tgatccgtcg tcagagactt acggttatat cacaaccact
    60781 cctaagtcag gctgggctaa tgtggatttg ttaggacaac tttcggctgc ttttaagatt
    60841 ccttttgatg tgacaacaga tgttaatagc tctgcttatg gtgaggtgct tgctcgtcct
    60901 ggtgtggaaa gtttggtcta ttacaccata ggtactggta ttggggcggg agctattcaa
    60961 catggccact ttattggggg attaggtcat acagaagcgg gtcataccta tgtcatgctc
    61021 catcctgatg atatggccaa gggattttta ggtgtttgcc cttttcataa agggtgtctg
    61081 gaagggatgg cagcaggtcc aagtattgag gccagaacgg gagtgagagg tgaacggctt
    61141 gatcaggaag ctgatgtttg ggacattcaa gctttctaca ttgcccaagc agcgctgcaa
    61201 gcaactatgc tttaccgtcc tcaagtgatt gtttttggcg gaggtgtcat ggcgcaagaa
    61261 catatggtgt tgcgcgtgca tgataaattc actacccttt taagcggtta tttgccagtc
    61321 cctgatttga cagactacat tgttactcca gctgttgctg ataatggctc cgctactctg
    61381 ggaaattttg ctttggccaa gttggctgca caaggaaaat agggtctgtt tttgcctgct
    61441 tgtctagtaa aagaaagcgc agtcagctag aacatgctat aataaacaga gattaacatc
    61501 agaagcgagg aatattatgt cagaaccatt atttctaaaa tcaaccatgc acgacagaat
    61561 ttggggtggc accaagttga gagatgtctt tgcttacaac atcccaagtg acaccacagg
    61621 tgagtattgg gctatttctg ctcatcctaa tggtgtttcg acagttacta atggacgtta
    61681 tcaaggacaa cctttaaata cattatatgc gcaagagcca gccttgtttg ggaatccaaa
    61741 ggaagaagtt tttccactgt taactaagat tttagatgct aatgactggc taagtgtcca
    61801 agttcatcct gatgatgctt atggacgtga acatgaagga gaattaggca agacagaatg
    61861 ctggtatatt atttctgccg aagagggttc tgaaattgta tacggccatc aggccaagtc
    61921 caaagaagac cttcgtgcca tgattgaagc aggtgcttgg gatgatttgc tgactcgtgt
    61981 cccagtcaaa gcaggcgatt ttttctatgt tccaagtggc accatgcatg cgatcggcaa
    62041 gggaattctt attttagaaa cccagcagtc atctgatacc acttatcggg tttatgattt
    62101 tgaccgtaag gacgttaatg gtaatcttcg tgaccttcat attgaaaagt ctattgatgt
    62161 cttaacgatt ggtaagccag aaaatagcgt gccagccact atggttttgg ataatatggt
    62221 agcaacaact ttagtctcaa cgccattttt cactgtttac aaatgggtga ctagtcaaat
    62281 ggttgacatg aaacaagctg cgccttatct tttagtgagt gttttaaaag gtcaaggaaa
    62341 actctatgtg gaccagaagg cttatgagct agaaaaaggc atgcacttta tcttgcctaa
    62401 cgatgttaaa tcgtggtcat ttgatggtca attagaaatg attgtgagtc accccaatca
    62461 actcgtttaa taccatggta tcaatatgag aaaaaaggat aaaattatga ctctctgttt
    62521 ctctgataac ttt
//
LOCUS       DAUGFP010000007        57100 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.007, whole genome shotgun sequence.
ACCESSION   DAUGFP010000007 DAUGFP010000000
VERSION     DAUGFP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57100
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.007"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            121..558
                     /locus_tag="VH663_000414"
     CDS             121..558
                     /locus_tag="VH663_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEQ8173643.1"
                     /translation="MNQNISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKT
                     LIGRLVEKKALGTYRQGRAYIYQALLDETLLQKEALATVLDGICQRQHTRLLLERLYD
                     LPMTLEEIEAFQELLEVKKENAVLEVPCNCLPGQCHCQKKETY"
     gene            558..2789
                     /locus_tag="VH663_000415"
     CDS             558..2789
                     /locus_tag="VH663_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEQ8173644.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKTVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPSFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGHP
                     NMDSLVALATSFAFAYSLYGVVEISLGRAHFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTYQAIQKLLSLKATDVRVWRNSQWQLLPLEEISYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGQGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADRVSGVFVPVVLVLALLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGVLFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQNLLT
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNATLMATLGIDLASVQSVCAKASGKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALQQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVITNLQSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKAMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            2803..3006
                     /locus_tag="VH663_000416"
     CDS             2803..3006
                     /locus_tag="VH663_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEQ8173645.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGEAKVT
                     GRPLTFLLKRALKDTKFELHKWS"
     gene            complement(3210..3980)
                     /locus_tag="VH663_000417"
     CDS             complement(3210..3980)
                     /locus_tag="VH663_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ8173646.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQSTQLLVAHENKE"
     gene            4217..4690
                     /locus_tag="VH663_000418"
     CDS             4217..4690
                     /locus_tag="VH663_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ8173647.1"
                     /translation="MFPNILFTEARTQPEFFDLVASHLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTMILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            4683..4988
                     /locus_tag="VH663_000419"
     CDS             4683..4988
                     /locus_tag="VH663_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ8173648.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQMSDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFEMPIPIVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            5014..6465
                     /locus_tag="VH663_000420"
     CDS             5014..6465
                     /locus_tag="VH663_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEQ8173649.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYADHNHLPVLVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIVSI
                     ITLIIFIAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            6723..7148
                     /gene="lacA"
                     /locus_tag="VH663_000421"
     CDS             6723..7148
                     /gene="lacA"
                     /locus_tag="VH663_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ8173650.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEEGYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            7163..7678
                     /gene="lacB"
                     /locus_tag="VH663_000422"
     CDS             7163..7678
                     /gene="lacB"
                     /locus_tag="VH663_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ8173651.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTYDHTRTHYPI
                     FGKKVGEAVVNGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEELNAN
                     VIGFGGKITGELLMCDIIDAFIKAEYKETEENKKLIAKIAHLETHHANQEDPDFFTEF
                     LEKWDRGEYHD"
     gene            7688..8141
                     /locus_tag="VH663_000423"
                     /pseudo
     CDS             7688..8141
                     /locus_tag="VH663_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            8188..9165
                     /gene="lacD"
                     /locus_tag="VH663_000424"
     CDS             8188..9165
                     /gene="lacD"
                     /locus_tag="VH663_000424"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ8173652.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            9362..9979
                     /locus_tag="VH663_000425"
     CDS             9362..9979
                     /locus_tag="VH663_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEQ8173653.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAAVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEKR"
     gene            9981..10805
                     /locus_tag="VH663_000426"
     CDS             9981..10805
                     /locus_tag="VH663_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8173654.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLDLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPRGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(10842..11399)
                     /locus_tag="VH663_000427"
     CDS             complement(10842..11399)
                     /locus_tag="VH663_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ8173655.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLKTKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            11692..12534
                     /locus_tag="VH663_000428"
     CDS             11692..12534
                     /locus_tag="VH663_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ8173656.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYVGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            complement(12663..13385)
                     /locus_tag="VH663_000429"
     CDS             complement(12663..13385)
                     /locus_tag="VH663_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173657.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            13587..15218
                     /locus_tag="VH663_000430"
     CDS             13587..15218
                     /locus_tag="VH663_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEQ8173658.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKPFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADGKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            15336..16484
                     /gene="nagA"
                     /locus_tag="VH663_000431"
     CDS             15336..16484
                     /gene="nagA"
                     /locus_tag="VH663_000431"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEQ8173659.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLGLHDGVFGEWIEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKNPRLEEFDAWQKAAK
                     GLIKKIALAPERDGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            16606..17445
                     /locus_tag="VH663_000432"
     CDS             16606..17445
                     /locus_tag="VH663_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEQ8173660.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLDHLGLDYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTQEDKKTIRYLSGMSATPNPDT
                     TSF"
     gene            17577..18110
                     /locus_tag="VH663_000433"
     CDS             17577..18110
                     /locus_tag="VH663_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEQ8173661.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            18482..19399
                     /gene="glyQ"
                     /locus_tag="VH663_000434"
     CDS             18482..19399
                     /gene="glyQ"
                     /locus_tag="VH663_000434"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEQ8173662.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEISDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDDE"
     gene            19777..21816
                     /locus_tag="VH663_000435"
     CDS             19777..21816
                     /locus_tag="VH663_000435"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEQ8173663.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPAH
                     TLTVLLDDEALDLDFLDIHSGRVSRGHRFLGKETTITSADSYEDDLRSQFVIADAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGNFEAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYARLAGEDAAVARAIREHYLPDAAGGALPETKVGAV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYANKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAVAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAVIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRLAILSDLVSKAKTIAAFNQLNTK"
     gene            21970..22227
                     /locus_tag="VH663_000436"
     CDS             21970..22227
                     /locus_tag="VH663_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEQ8173664.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            22354..22746
                     /locus_tag="VH663_000437"
     CDS             22354..22746
                     /locus_tag="VH663_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEQ8173665.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            23106..24632
                     /gene="glpK"
                     /locus_tag="VH663_000438"
     CDS             23106..24632
                     /gene="glpK"
                     /locus_tag="VH663_000438"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEQ8173666.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQFA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            24648..26486
                     /gene="glpO"
                     /locus_tag="VH663_000439"
     CDS             24648..26486
                     /gene="glpO"
                     /locus_tag="VH663_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEQ8173667.1"
                     /translation="MEFSKETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDEIRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQI
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVINEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGHQEG"
     gene            26488..27189
                     /locus_tag="VH663_000440"
     CDS             26488..27189
                     /locus_tag="VH663_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ8173668.1"
                     /translation="MDIFGEFLGTVLLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAYLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            27298..28644
                     /locus_tag="VH663_000441"
     CDS             27298..28644
                     /locus_tag="VH663_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ8173669.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNHRIIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QVNIYAIGDMVSLHFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGDSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDADLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            28634..30133
                     /locus_tag="VH663_000442"
     CDS             28634..30133
                     /locus_tag="VH663_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8173670.1"
                     /translation="MKIEDLMDKERRAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHKHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYFEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGVILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMDQETDFSQQLSDDILALLIFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVCLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            30259..30903
                     /gene="fsa"
                     /locus_tag="VH663_000443"
     CDS             30259..30903
                     /gene="fsa"
                     /locus_tag="VH663_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ8173671.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMMQHPLTTNGLKTFMEDWASFKK"
     gene            31121..33106
                     /gene="tkt"
                     /locus_tag="VH663_000444"
     CDS             31121..33106
                     /gene="tkt"
                     /locus_tag="VH663_000444"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEQ8173672.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVDHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAAYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLDGIIIATGSEVKLAMDT
                     QEALEAEDIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            33299..33565
                     /locus_tag="VH663_000445"
     CDS             33299..33565
                     /locus_tag="VH663_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ8173673.1"
                     /translation="MEKKRQRLYDVIRQAYDYPENRENVALSQLLLAASNRLIKHSTP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            33599..34333
                     /locus_tag="VH663_000446"
     CDS             33599..34333
                     /locus_tag="VH663_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173674.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            34338..35966
                     /locus_tag="VH663_000447"
     CDS             34338..35966
                     /locus_tag="VH663_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173675.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQEKHPKENFKAYKSMM
                     RQQALMIAIFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGTVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVVQAPFSTAALLNFWLPLLLILAMVYGIVTKVMPTYYR
                     EAFYISNENKVKQTKKPVNRPHQNQSLAQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     EFMYRRDYRLLDLKWQDMTQLFTRGNGQWLTMGLIFGNLIVTGVVGFGAVIIANIIQQ
                     PLLISVLLSCLTLMVLGLAQLWIQKTFWKSLERL"
     gene            36031..36852
                     /gene="proB"
                     /locus_tag="VH663_000448"
     CDS             36031..36852
                     /gene="proB"
                     /locus_tag="VH663_000448"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEQ8173676.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKSPTIYEDAQLRSHVAVITQEIIASAGGAGSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD"
     gene            36845..38095
                     /locus_tag="VH663_000449"
     CDS             36845..38095
                     /locus_tag="VH663_000449"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEQ8173677.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNCFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAMAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDAFHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATKIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            38115..38330
                     /locus_tag="VH663_000450"
     CDS             38115..38330
                     /locus_tag="VH663_000450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173678.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFVFLEMVC
                     WSAGSNVVSKFNQLLIDLQVTLQNCRR"
     gene            38373..38537
                     /locus_tag="VH663_000451"
     CDS             38373..38537
                     /locus_tag="VH663_000451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173679.1"
                     /translation="MSWDKIFDIWLDNLLTDCCVIKEAKNLLQAVETLEAAAKSVLAK
                     LADSEQKEQD"
     gene            38561..39574
                     /gene="rsmH"
                     /locus_tag="VH663_000452"
     CDS             38561..39574
                     /gene="rsmH"
                     /locus_tag="VH663_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEQ8173680.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLQSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPTFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            39579..39902
                     /gene="ftsL"
                     /locus_tag="VH663_000453"
     CDS             39579..39902
                     /gene="ftsL"
                     /locus_tag="VH663_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEQ8173681.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            39906..42161
                     /locus_tag="VH663_000454"
     CDS             39906..42161
                     /locus_tag="VH663_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ8173682.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGTGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            42163..43173
                     /locus_tag="VH663_000455"
     CDS             42163..43173
                     /locus_tag="VH663_000455"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEQ8173683.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLAGGLMFYFLHVSPSGISSINVFGYQLSLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGTMIGALLGFFL
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            43331..44674
                     /locus_tag="VH663_000456"
     CDS             43331..44674
                     /locus_tag="VH663_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8173684.1"
                     /translation="MSFKDYHFKQYIQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDETKAEVQVVITAPSRELATQIFDACKQIAKHFQEEIRL
                     ANYVGGTDKLRQIEKLKGSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERKAKKQHF"
     gene            44869..45672
                     /locus_tag="VH663_000457"
     CDS             44869..45672
                     /locus_tag="VH663_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ8173685.1"
                     /translation="MTSVFLTSGWAFYDHLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKCLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            45672..46415
                     /locus_tag="VH663_000458"
     CDS             45672..46415
                     /locus_tag="VH663_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173686.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            46519..46743
                     /locus_tag="VH663_000459"
     CDS             46519..46743
                     /locus_tag="VH663_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEQ8173687.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            46807..47724
                     /gene="trxB"
                     /locus_tag="VH663_000460"
     CDS             46807..47724
                     /gene="trxB"
                     /locus_tag="VH663_000460"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEQ8173688.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDSEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
     gene            47893..49272
                     /locus_tag="VH663_000461"
     CDS             47893..49272
                     /locus_tag="VH663_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ8173689.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            49443..50903
                     /locus_tag="VH663_000462"
     CDS             49443..50903
                     /locus_tag="VH663_000462"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEQ8173690.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPENFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAIIGGADATSNVRAGKRFGIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVRNFDAIPLLVDIFVKGELVYQLPTLSEIKAYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMALIDNIRKDTHGKSDLK"
     gene            50900..51724
                     /gene="nadE"
                     /locus_tag="VH663_000463"
     CDS             50900..51724
                     /gene="nadE"
                     /locus_tag="VH663_000463"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEQ8173691.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREETSDQAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQAVALQQAGIEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALEVTYQDIDDYLEGKLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            51908..53245
                     /gene="pepC"
                     /locus_tag="VH663_000464"
     CDS             51908..53245
                     /gene="pepC"
                     /locus_tag="VH663_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEQ8173692.1"
                     /translation="MSALTETFTEQLFAHYEASAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQY"
     gene            complement(53710..55875)
                     /locus_tag="VH663_000465"
     CDS             complement(53710..55875)
                     /locus_tag="VH663_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ8173693.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDTSNSTNTDNNSNHPSTDDKKTTH"
     gene            complement(55862..56470)
                     /gene="recU"
                     /locus_tag="VH663_000466"
     CDS             complement(55862..56470)
                     /gene="recU"
                     /locus_tag="VH663_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEQ8173694.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            56551..57066
                     /locus_tag="VH663_000467"
     CDS             56551..57066
                     /locus_tag="VH663_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEQ8173695.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNQEVLSQFRAVDF
                     VKYYFPNYEQPTQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
BASE COUNT        17145 a        10276 c        12402 g        17277 t
ORIGIN      
        1 atcataaatg aaaaaattgt caacttaaga aataaaatca ttagaaactt tatatattta
       61 caaatgtaga taaatatgat ataatatatc tacaaatgta gattaaaagg ttgtgagttg
      121 atgaatcaaa atatttcagc tgccgaatgg gaagtcatgc gtgtggtgtg ggccagtggt
      181 gatattaaaa gtagtgatat tattactatt ttaaggaaaa aatatcaatg gtcagattct
      241 accattaaaa ccttgattgg gcgtttggtg gaaaaaaagg ctttgggaac ttaccggcaa
      301 ggaagagctt atatttatca agctcttttg gatgagactt tgctacaaaa agaagcacta
      361 gcgactgttt tagacggtat ttgtcagcgg caacacacgc gtctacttct tgaacggctt
      421 tatgacttac cgatgacctt agaggaaatt gaggcattcc aagagctact agaagtcaaa
      481 aaagaaaatg cagttcttga ggtgccgtgt aattgcctgc ctgggcagtg ccactgtcag
      541 aaaaaggaga cgtattaatg gctaaagaga tatttttagt ggagggaatg agttgtgcct
      601 cttgtgctct aacaattgaa aaaacggtca atcaattgcc tgaagtagaa caggctgttg
      661 tgaatttagc gaccgagaaa ctcacggtta cttatcaaga tgacgattta gaaacggcca
      721 aagttattca agcggtaaaa gctgctggtt atggtgcaca agtctttgat gaacatgcaa
      781 aacacatgca aacaggtcgt cgtgacgaag acctaaaggt tatttggtct caggtcttgt
      841 ggtctgccct attcaccatt cctatattat acctagctat gggtcacatg gtgggcttat
      901 ggcttccttc atttctgcaa ccagatcgct atcctttcgt ttacagcttt agtcagctac
      961 tcttgacgat tccagtctta gtattaaatc gtcattatta ccataatggg ttcaaagctt
     1021 tgttcaaggg acatcctaat atggactcct tggtagcttt agcaaccagc tttgcttttg
     1081 cttatagtct ttatggagtg gttgaaatca gtctgggccg ggcgcacttt gtgcactccc
     1141 tttattttga atcggtggta gtgattttga cactcattgg tctaggaaag tattttgaaa
     1201 gtcgttctaa gggccgtact tatcaagcta ttcaaaagct cctatccttg aaggctactg
     1261 atgtgagggt atggagaaac agccaatggc aattgttgcc gttagaagag attagctatg
     1321 atgaccttgt cctggttcaa ccaggtgaaa aagtttctat tgacggcatt atcgtggaag
     1381 gacatagcag tttggatgaa tcctttttaa ctggggaaag tttgcctgtt gaaaagggag
     1441 aaaaagataa tgtctttgcg ggcagcttga atggtcaagg cgccctcacg attaaaccag
     1501 ataagcttgg gaatgatacc ttgttagcac aaattatcca actagttgag aatgctcaag
     1561 aaaataaagc acctattgca gccattgctg atagagtctc aggtgtcttt gttccggtgg
     1621 ttttggtatt agctctgtta acaggggtgt tttggttggt attcatgcag gaaagtcttc
     1681 gttttagttt gacaacagcc atcgctgttc tagtcattgc ttgtccttgt gcgcttggtt
     1741 tagcaacccc gacagcgatt atggttggaa cagggcgtgc agctgaaaat ggtgttcttt
     1801 ttaaaggtgg tgacatctta gaacaagccc atcaggtaga tacggttgtt tttgataaaa
     1861 cggggacgat tacggagggg aaaccaagtc ttcaaaacct cctaactttt tcgggagatg
     1921 atcaccttat cttgcaagaa gctgcttcct tggaggccta ttctcaacac cccttagggg
     1981 aggctattgt ccgtgctgct caggaagctg gttacgatag tctaccagtg gaagagtttg
     2041 agtctttaac cggcctagga gtcactggtc agcttgaagg gcgtcgtttg gcgattggaa
     2101 atgctacttt aatggcaacc cttggtattg atttagcttc tgttcagtca gtttgtgcta
     2161 aggcttcagg gaaaggtcaa acccttgtat attatgctaa agaggggcag ctaagagctc
     2221 ttttcagtat tgctgacgct gtcaaagaag atagtcaagc taccgtcgaa gctttgcagc
     2281 aactgggaat tcacaccatc atgctaacag gtgaccacga tgctaccgct aaggctattg
     2341 cctctcaagt ggggattaca gatgtgatca gtcaagtctt acctgatcaa aaagcaggcg
     2401 tgataacaaa cctccaatct caagggagaa aagttgcaat ggtaggtgat ggtattaatg
     2461 atgctccggc attggctgta gcagacattg ggatagctat ggggtcaggg acagatattg
     2521 ccattgaatc ggcggatgtg attctgatga aacctgatat gctggatttg gtgaaagcta
     2581 tgtcattgag tcgagcgact atgaggattg tcaaggaaaa tttattttgg gcattcatat
     2641 acaatgtcct catgattcca gtggccatgg gcttacttca tctttttgga ggtccactat
     2701 tgaacccgat gttagcaggt tttgccatga gttttagttc agtatcagtt gttttgaatg
     2761 ccctgcgatt aaaaggaaaa accatttgaa ggagaaaacg aaatggaaaa acactatcag
     2821 gttacaggaa tgacgtgtga cggttgtgca agaacggtta ctgaaaaatt atcagctgtc
     2881 cccggtgtgc aatcggtcca ggtaaatttg gaaaaaggag aggctaaggt gacaggacgt
     2941 cctttaactt tcctactcaa gcgtgctttg aaggatacca aatttgaatt acataaatgg
     3001 tcttaggtta ttttggtagc cttacttcat ctcaaaagag gttccagttt tttaatctgg
     3061 gcttcttttt ttgtggtcac tcataaagac tctgacgtta gcaggtgtgt tccgagcaaa
     3121 acaaaaacac ctctctgggg gcaaatcact tttgccagaa ggtgtttaag gacgatgcgc
     3181 tgacaggctg tccattcttg atgaaacggt tactccttgt tttcgtgggc tactaataat
     3241 tgtgtggact gactataacg ttcgatgacg tcctcagtta tttgcgaatc tgtaacaaca
     3301 gcatcaatgt tgtttaaatg gtaaaaaatg aagaagtcgt aggcattgaa tttttggtta
     3361 tctaccaaga ggtatttttc aatagaatta ttaaaagcta gcttttgaat ctctccttca
     3421 gtttcgctat aagttgcaat atcgttttta taaatgccat tacagctaat aaaagccttg
     3481 gcaaatttta aggatgagat attttgacta gcaagggacc ctacaaaagc tcctgtaata
     3541 gatcggtatt caccaccgat aagaatcagg tcaatggttt cgctgtcctg aaggatgtta
     3601 aagacaggga ggctattggt cacaatgcga atctgacgat tttttaattg ctcagcgaaa
     3661 cattctaaag ttgttcctgg accaataaag attgtttcac catcattgac tagttgtgtc
     3721 gcatatgaag ctagttccca tttttccttt gtttgaacgg tttgtttttc aatatttgac
     3781 cgctcttttt tattaggcat ggtaatactt tgtgcacctc catggatgcg gatcaatttg
     3841 ccggcttttt ccagttcatc taagtctcta cgaactgtca tatcagaaac gtttaaagtc
     3901 tgaataatat cattcacagt aacaatacct tgctcattga ctatttcggt aatttttaga
     3961 agtctttctc tttttaacat ctaaggggct cctttctata gtttatttac ttatgtttat
     4021 catagcatat agaccttcat atttcaataa aacaaaataa acaaaacaaa aacaaacata
     4081 tagaacaaaa aatattgcgt aaaaatgttg aattatgttg aaggttatac tataatgatt
     4141 ttgtaaacgg ttaccaattt gttgatgatg agatgacagt tgttaaaaat tgatacttat
     4201 agaaaggcgg tagggagtgt ttcctaatat tctatttact gaagcaagga cgcagccaga
     4261 gttttttgat cttgttgcta gccacttgga aaaggttggg tatgtgacgc aagagtacca
     4321 tcaggcttta gtggagcgtg aggcggtatt tccgacagga ctcaaagtgg acctcaaaga
     4381 tgggtcagat attttgtatg cggctattcc gcatacagaa accaagtatt gcttggttga
     4441 tcaagtggtc tatgttagaa atagtcaagc ccttaccttt aagcacatga ttaatccaga
     4501 ggaagactgt ttggtaacgg atttcttctt tattatcaat agtcaaaacg agggtcaaac
     4561 gatgattcta tccaatctca ttaccttctt tattacgaaa ggcaatctta gctatctagc
     4621 cagtctaaaa gatgataaac aagctatttc aaactattta atagaaaaag gagtgtttca
     4681 gcatgattaa aattctagca gcctgcggtg caggggttaa ctcaagccat caaattaaag
     4741 atgcgattga aactcagatg agtgaccgtg gttacgatgt tcactgtgat gcggtgatgg
     4801 tcaaagacat cacagaagaa ttggtgtctc gttatgatat ttttacgccg attgcaaaaa
     4861 ctgacttggg atttgagatg ccaattccca tagtagaagc aggaccgatt ctctatcgta
     4921 tcccaatcat gagtgagcct gtttttgctg aattagagcg cgtgattaaa gaaaatcatt
     4981 tgcagtgagt taataaaaac ataaggagac aagatgatgc aacctttttt agacattatt
     5041 aacaagattc tgggatttcc tattcagttg ggatcaggtg tggtcatgct gattgtcatg
     5101 acaggtttag ccatgatttt cggggttaaa ttcacaaagg cactggaagg cgggattaag
     5161 ttagctatcg ctcttacagg tattggagcc attattggga ttttgacggg ggctttttca
     5221 gagtctttac aggcctttgt taaaaataca gggatcagtt taaacattat tgatgtcggt
     5281 tgggcaccgc ttgcaacaat tacctggggt tccccttata ccctttattt cttgctggtg
     5341 atgctggtgg ttaatattgt catgattgtc atgaaaaaaa cggacaccct tgatgtcgat
     5401 atttttgata tttggcattt gtctatcaca ggtctattga ttatgtggta tgctgatcac
     5461 aaccatttgc cagtccttgt gtctcttttg attgctactg tggcagttat tcttgttggg
     5521 gtattgaaaa tcattaactc tgatttgatg aaaccgacct ttgatgatct tcttgggaca
     5581 gggtctcaat caccaatgac ttcaactcac atgaactaca tgatgaatcc aatcattatg
     5641 gtcttagata agatttttga caaggtcttt ccggggctgg ataaatatga ctttgatgct
     5701 gctaaattga acaaaaaaat tggcttctgg ggctctaagt tctttatcgg aatggctcta
     5761 ggttttgtga ttgggattat gggagatcct catttcactg ttgaatctat caaaaattgg
     5821 tttggtctag gatttacagc tggtgcctgc ttggaacttt tctctttgat tggctcttgg
     5881 tttatcgcag ctgttgagcc tttgtcacaa gggattacca attttgccaa tgctagaatg
     5941 catgggcgtc gtttcaatat cggtttggac tggcctttca ttgctggtcg cgctgaaatc
     6001 tgggcctgtg ccaatattct tgctccaatc atgttgattg aagccgttct cctatcaaaa
     6061 gttggcaatg ggattttacc attggcggga attattgcta tggggatgac accagctctt
     6121 cttgtggtca cacgtggtcg tttgattcgc atgattattt ttggttccct cttattgcca
     6181 ttattcctct tgtcaggaac tatgattgct ccatttgcga cagagttggc taaaaaagta
     6241 ggggcttttc cagcaggtac aagcgctggt tccttgatta cgcattcaac acttgaagga
     6301 ccaatggaaa aaatctttgg ttatgtgatt ggtcaagcga caacaggtca gattgtctca
     6361 atcatcacgc taatcatctt tattgccatt tacctaggtc tctttgcttg gtatgctaac
     6421 caaatgaagg cacgtaacgc tgagtatgcg aaaacaatga aataaaatca ggtcaagtga
     6481 ctgactaaaa aattagggag aatagcagat aaccgcttaa ggtattcctg ccttatcccc
     6541 taacgtgaaa agagtatttc tcatgtaagg caaggcgctc tgtgggaacc cacaagtgct
     6601 ttgtcttgaa agaaataatg gaagacaact aagactgctg gcaatcatct tgatctaagg
     6661 acaggtaatc atgtccccct tctctagatt ggcaagcgtt ttgggttaga aaaggaaaag
     6721 ctatggctat tattttaggt gcagatgcac acggaaatgc attaaaagaa ttgatcaaaa
     6781 gttttcttca agaagaaggc tatgacatta ttgacgtgac tgatattaac agtgatttta
     6841 ttgataacac attagcggtg gctaaagccg ttaatgaagc agaaggtcgt ttaggcatta
     6901 tggtggatgc ttacggagca ggtcccttta tggtagccac aaaactaaaa ggaatggttg
     6961 ctgctgaagt gtcagatgaa cgctctgctt acatgacgcg cggtcacaac aatgcccgca
     7021 tgatcacaat aggggcagag attgtaggtc cagaattagc gaagaatatc gtcaaaggtt
     7081 ttgtgactgg tccctacgat ggtggtcgtc atcaaattcg tgttgacatg ctcaataaaa
     7141 tggcctaaag gaggataaag tgatgaaaat tgcgattggt tgtgaccata ttgtcaccaa
     7201 cgaaaaaatg gctgtttctg attttttgaa atctaaaggc tatgacgtta ttgactgtgg
     7261 cacttatgac catacaagaa cccattaccc tatttttgga aaaaaagtcg gcgaagctgt
     7321 ggttaatggt caagcagact tgggtgtttg tatttgtgga actggagttg gcattaataa
     7381 tgccgtgaac aaggtaccag gtattcgttc agcccttgtt agagatatga caacagcgct
     7441 ttatgccaag gaagaattga atgccaatgt gattggcttt ggtggtaaaa tcactggtga
     7501 acttcttatg tgtgatatta tagatgcttt tattaaggca gaatacaaag aaactgaaga
     7561 gaataagaag ctaattgcta aaatagccca cttggaaact catcatgcta accaagaaga
     7621 tcctgatttc tttacagaat ttttagaaaa atgggaccgt ggtgagtatc acgattagga
     7681 agaagagatg agtttaacag ttaccttaaa tccttctgtc gctatttcct atcctttaga
     7741 ttgtctggct atggacacgg ttaatcgtgt tgacagaacg gcaaaaacag atggtgataa
     7801 ggagctaaat gtgaccagaa tgttagcaaa agctggtcag ttatggcaac aggatttagt
     7861 ggtggtaaac tgggtgactt tatcattcat cagttacaag aacagggcat ttctaaccaa
     7921 tttttcaaaa tcaaagggga aacgagaaat tgtattgctg ttttacatga aggcatgcag
     7981 acaaaaattt tggaagctgg tccctacagt gatgtggacg aagccgaagt ctctttgagc
     8041 cacatgagta ctattgctaa gccgtttgat gtcttaactt ctcaggcagt ctgcctaaga
     8101 gactagctac tcactattat caagatttga tgactatggc aacttttaaa caattatatc
     8161 agcaacttca agtaagagag gtatcacatg acaatcacag caaataaaag acattactta
     8221 gaaaaagtga gccatcaagg aatcatatca gctttagctt ttgatcaacg aggtgcactg
     8281 aagcaaatga tggcagcaca tcaagaagga gaagcgacag ttacacagat agaaacattg
     8341 aaagttctcg tttctgagga attgacgcct tatgcttcat ctattttatt agatccagaa
     8401 tatgggttgc tggctactaa ggtaagagct aatcagacag gcttactgtt agcttacgaa
     8461 aaaacaggtt atgatgctac aaccactagt cgtttacccg attgcttagt ggaatggtcg
     8521 gtcaaacggt tgaaagcagc aggtgcagat gctattaaat ttttgctcta ttatgatgtt
     8581 gatggagatg aacaaatcaa tttacaaaaa caagcttata ttgaacgcat tggttcagaa
     8641 tgtacagctg aagacattcc tttttttctt gaacttttga gttatgatga gagaatatca
     8701 gataacaaca gtgcggcata cgcaaagctt aagccacata aggttaacgg tgctatgtca
     8761 gtattttcgg ataagcgttt tggcgttgat gtgctaaaag tagaggttcc tgttaatatg
     8821 gcttatgtag aaggttttac tgaaggggaa gtccattaca gtcaagctga ggctattaaa
     8881 gcttttcaag accaagaagc tgccagccac ctcccttata tctatttgag tgcaggcgta
     8941 tctgccaaac tttttcaaga gacattatac tttgcagcag cagcaggtgc tcaatttagc
     9001 ggtgttttgt gtgggcgtgc cacgtgggca ggatccgttc cagtttatat tactaaagga
     9061 gaggacgaag caagaaagtg gctatgcact gaaggcttcc aaaatattga cgagttaaac
     9121 agggtactcg aagagacagc tagcccttgg acggaaaaaa tctaaaacaa ccttatccta
     9181 tcaagcagct ctcattgatg tgataagata agtatgggga taattgtttg aatcttttgt
     9241 agcaaggatg aaaatgggtt ttatccttgc ttttttatcc ttacatttta tctttttcta
     9301 cttatttttt aaaggaaaac gtggtaaact aaaagaagac taaaagacaa tagggtagct
     9361 tatgacaatt gatatgtggg aaattttaaa tatcatcggg accattgcct ttgctctttc
     9421 aggtgctatt gttgccatgg aagaagaatt tgatatttta gggattttta ttttaggttt
     9481 cgtgacagcc tttggaggcg gagccattcg aaacacccta attggtttac ctatagaggc
     9541 cttatggggg caaaagccag aattcacttg cgctttcttt gctatggttt tgattatgct
     9601 ttttcctaag ctaatagcta gaggttgggt acgggcagct gttttgactg atgccattgg
     9661 tctggcagct tttagtgtcc aaggagcctt gcatgctgtc cgtcttaacc agcctttaag
     9721 tgctgtcatt gtagcagctg tgttgactgg tgcaggcggt ggtgttgtca gagatattct
     9781 ggcagggcga aaacctagtg ttttgcgtag tgaaatctat gctgggtggt ctattcttgc
     9841 agctattgtt ttgcatttta aactagcaga ttcaacgatt gaatgttatg ccttggttgt
     9901 tttactcacg actttacgca tgattggcaa ccgtaaaaaa tggaacctcc ctaaaatcaa
     9961 atggaaagag aagagatagg atgattcaac taattgcaat tgatttggac ggcaccttat
    10021 taaaccagga caaacagatt ccaaaagaga atatcacagc catccaagaa gcagctcagt
    10081 caggcttaaa aattgtctta tgcacaggcc ggcctcagtc agggacaaga ccctattttg
    10141 accaattaga tttgacacaa gaggaattcc tcattatcaa taatggctgt agtacttata
    10201 gtagtcctga ctggcaatta cgccattcaa aaatgttgaa ggtttcagat attgaattgc
    10261 tggaagagct aagccaaagc tttccagata tttacctgac tttaactgag gaaaacgatt
    10321 atttggtgct tgaagaagaa gtgccagacc ttgtgcagga agatggagat ttggtattta
    10381 ccattgtcaa gcctgtctcc ttagcagagc tgtcggacac tcctcgcctg atttttcaag
    10441 ccatgtactt aggagaaaaa gctgcgctag atgcctttga acgtgcggtg agaaatcagc
    10501 ttagtcagag tttccatgtt gttcgtagcc aagataatat tttagaaatt ctccctcggg
    10561 gagtcagcaa ggcatcggct ctcaaagaat tagtagaaga tttgggttta actgctgacc
    10621 aagtgatggc tattggggat gctcccaatg acattgagat gttaacttat gctggtttag
    10681 gagttgctat ggaaaatgct tcagcagcta tcaagccatt agctgacaaa gtcactttga
    10741 ctaatgatat ggcgggtgtg gcccaggcta tccgtcagtt tgcacttgca gctcaaaaag
    10801 actaactatt atataaaaaa aggttcccaa aaggaacctt tttacgttaa tagaggcaag
    10861 cccatagcag ccatttcttc ggcaggaacg atcatgtcat ctgaaatcca gtttgtcaag
    10921 acagaaacaa tagcattgct ccaaaaaagg cgcttatagt gcgattgttt tttgtctttt
    10981 aatttttgat aagctttcag acgtttagtg actaatcgtg ttagaagatg gtgcaggtga
    11041 tgtttgaaaa tcattttaag aagctgagct tcttttttag cttcagtaaa aagatagagc
    11101 caggcttgat aagcctgagt tttaatatca aattgtttta gtccacgaaa aatacgacgg
    11161 ataaccccta ctaacagttg ttctataatg gcttctttag aggtataatt acgataaaaa
    11221 gcgttacgag atactcctgc tttcgtcacc aattctgaaa tcgtaatatc acctaaaggt
    11281 tttgttttta gaagatttag taaggcgatt tccatagatt cacgtgctat gcgtcgtgtt
    11341 tctttattaa attgtgttaa gttttgaagt gatctttttg agagtgcttt atcagtcatc
    11401 acaaatacct ttcaagtgtt acatctgatg agttatcagt agcaaaaacc gacttaataa
    11461 tgatataatt ttatgggaaa agtcttagtt tgtcaactaa agttagttat caggagcctc
    11521 attacctccc tgtatagctc tattggatga ctttactaac tattgaaaag gctagtaata
    11581 aaacatagga tttaatgact agttattacc attagcctag acatcttagc gaaggcttaa
    11641 taatctggtc tttgttaccg aacacaaatt agagaaaaag aaggataaaa aatgacttgg
    11701 aaaattgtaa cagattcagg atgtgatctt agaagtttga ctagacaatc caaagagttg
    11761 cgctttgaac gtgtgccttt gaccctacag attggcacag aaatttttag agatgatgat
    11821 ggattagata tcgataatat gatgacaact atgtaccagt cgtctaaagc aacgacatcc
    11881 agctgtccaa gcccagaagc ctttttgcag gcctatagag gtgcagataa tgttatcgtg
    11941 atgacaatta cagggacctt atctggcagt cataatagtg cacgactggc taaaaatgaa
    12001 cttcttgaag aaaatcctaa tgtcaatatt cacctaatcg attctttatc agcaggtggc
    12061 gagatggatt tgctcgtact ggagcttgag cggttgatca atcttggatt atcctttgaa
    12121 gaagttgtca aacagatcac agcttatcag caaaaaacac gtttgatttt tgtgcttgcc
    12181 aaggttgata atttggttaa aaatggtcga ctgagtaaat tagtaggtaa agtaattggc
    12241 ttactcaata ttagaatggt tgggaaagct agcaataaag gaacgcttga acttcttcaa
    12301 aaagcgcgtg gtcaaaaaaa agcagtttct gctttgatag aagaaattca aaaagagggt
    12361 tatgtaggcg gaaaagttta cattgctcat gctcaaaatc caaaaatttg tgagcagatc
    12421 agtgaaaaaa tcaaatcttt atatcctgat gctgtgattc aaactggaag gacctcaggt
    12481 ttgtgtagct tttatgctga agacggagga cttttaatgg gctacgaaat ttaatgctat
    12541 ctaacaaaac aaaaagctag actcttttga tttgagctag cttttttaag atgtcaggaa
    12601 gactaatcac taagatagcc gtctaaaacg tttattgatt gattttacga aaaacttttg
    12661 tattactctt ttgatgattg cttgtctcct gagtcggaag gttctgaaga ggtctgaggc
    12721 ggaattggat caggatgact aggttgtggc gtaacatctg aagctgaaga ttctgagctt
    12781 gggtctaaag taggtgactg ggaattatca taagtatctg agacgtctcc tgggaaaggt
    12841 gtccagtaag agttatctgt tatttctcca ctttgtcttg gaaattcatc aataatgggt
    12901 ttcgctggta aaatagcgtc taaccgagat tgtaaagcgg cttttttacc tttgttactt
    12961 aagtcgttaa ctgctttttg ggcgatatct ttaagatttt gattgagagt ctcctcagca
    13021 gctttaactg cttgagtagc tgcatcttct tgctcttgct ttgctttggc aacgtttagc
    13081 tgttcttgta gcgttttttt cttagaagat tctgctaatt tatcaacagc tttctgggct
    13141 tcttggatag cagctgcagt ggctaaacga tcagcgtcat taactgcttt aactgctttt
    13201 tttagtaaac tagcagcact tttagaatgt ttagctacct tatcttgcgt ttgagggtgg
    13261 gatccaaaaa taaggcagga gccacctatt agagtgatag ttagaacact caacatccaa
    13321 taaagcgcct ctttttgtga tttagtcatg gaatctcctt tatgatattg tttcatcgta
    13381 gccatattat acctctaatt aaacgaaatg aaaagatttg tgcaaaaaca aatgatgtaa
    13441 aatatcggaa aagcctaaca ccttatcaaa ccaataatat gcaaatgttg acatcggtat
    13501 acacagaaac gcttaaaaaa accttgtgat aaagaccgta atacattaaa atagaataga
    13561 ctaaaaaaat gagcgaggac aatattgtgt cagtaaattg gcaagacatt gcatttcatt
    13621 tctttggagg cttaggtctc tttcttttca gtatcaagta tatgggagat ggtctgcaac
    13681 aagcagcagg agataaacta aggtattaca ttgataaata taccagtaat cccttttttg
    13741 gtatcttagt cggaattgcc atgtctgcct taattcagtc cagttcaggc gtcactgtga
    13801 ttacggttgg tttggtttct gcgggtttgc taaatttgcg acaagccatt ggaattgtta
    13861 tgggggctaa tattggaacg actatcactt cgtttttaat tggttttaaa ctaggagatt
    13921 acgccttgcc catgattttt attggtgcag cctgtctttt ctttacctct aataaaaagc
    13981 tcaacaattt tgggcgtatc atttttggtg tcggaggaat tttcttctcg ctgaacctaa
    14041 tgggagatgc catggatcct ttgaaatctg tgtcagcctt tcaaaattat ttagcgacat
    14101 taggtgataa acccttccaa ggtgtcttta tcggaacggc tttgaccatg ttgattcagt
    14161 catcggcagc cattatcggg attttacagg gtctgttttc aggcggtctt ttgaccttac
    14221 agggggctat tcctattttg ttaggctcaa atataggaac ctgcattaca gctgtcttag
    14281 cagctatagg ttcaaatatt gctgctaaac gtgtggcagc agctcatgtg ttatttaatt
    14341 tgatcggtac aattattttt atgattatcc tagtcccatt cacatcattg atgttatggc
    14401 tacaatcaaa gttgagttta acgcctgaga tgactatcgc tttttctcac ggttctttta
    14461 atatcactaa taccatctta ttaattcctt tcattagtct actagctatg attgtgacta
    14521 gactcattcc aggagaagat gaagtggtca aatacgaagc cctttattta gatcgcttac
    14581 ttattaccca agccccttct atcgcactag gaaatgccca caaggaattg gttcatttag
    14641 cctcttatgc gattcaagct tttgaagctt cttacagtta tattatgaca gctgacggta
    14701 aatttgggga aaaggtcaag cgttatgaaa gagcagttga taccatcgat gaggagttaa
    14761 ccacttattt ggttgatatt tctaacgaag ccctcagccc aagtgaaaat gaagtcttgg
    14821 ctggtattct tgactcttca cgtgatttgg aaagaattgg agatcattct gagtctttag
    14881 gcattctaat tgaaggcatc atatctaaac aaattggttt ttcaatatct gctcgtcaag
    14941 aattgacaga gatgtatcaa ttgacgcatt gtttaacact ggatgccatt agagctatcg
    15001 ttgatagtga tacagacttg gcgcaaacta ttgtgactcg ccataaagaa attgaagaaa
    15061 aagagcgacg attacgtaaa acccacatta agcgcttgaa ttgtggagaa tgtacggcac
    15121 aagcaggtat taattttatt gatattattt cccactatac ccgcatcaca gatcacgcct
    15181 tgaatttagc agagaaagta ctttcacacc agctttaaaa aaagagaaaa cgtgaatgat
    15241 catccacgtt ttcttttttg aatccaaaaa gtggtatata ccatttacaa agtaaagaat
    15301 aaggcgtata ataaggaaaa aagggaggaa aaaagatgac atgctatctt aaagcagatt
    15361 gtttttatta cccaacagag gtgaaaccgg ctggctacct aggtcttcat gatggtgttt
    15421 ttggggagtg gatagaaatt gttccggcag atgcgcaaat cattgactac accggttatc
    15481 aaattgcacc aggtcttgtg gatacgcata tccatggcta tgcgggagct gatgtcatgg
    15541 ataattctgc ccaagggatt catcaaatga gtgaaggact attagcaaca ggagtaacgt
    15601 cttttttacc gacgacctta acttctacct ttgagcagtt agaaaaagta tcagggacta
    15661 ttgcttcagt tgctgatcag gtaaaaggtg ctaaaataca aggtatttac tttgaaggtc
    15721 cttattttac cgaagaatat aagggtgctc aaaacccaag ttatatgaaa aacccacgct
    15781 tggaggaatt tgatgcttgg caaaaagccg ctaaagggct catcaaaaaa attgcccttg
    15841 ctcctgagcg tgatggtgtc aaagaatttg tgtccgcagt gacaaaacaa ggcgtaacag
    15901 tggccttagg gcattcaaac ggtacttatc aagaggctaa agaagctgtt caagctggtg
    15961 ctagtgtttg ggttcatgct tataatggca tgcgaggatt gacccaccgt gaaccaggga
    16021 tggtgggagc ggtttataat cttccaaata cttacgcaga gttgatttgt gatggccacc
    16081 atgtctcacc aattgcttgt gatattttga tgcagcaaaa agggcatgat catgtagcca
    16141 tgattacgga ctgtatgcga gcaggtggtt caccagatgg tgattacctc ttgggggaat
    16201 tctctgtggt tgtggcaaat ggcactgctc gtcttaaaga gagtggaaac ttggcaggat
    16261 ctattttgaa attaaaagac ggccttagaa atgtagtggc ctggggaatc gcaactccgg
    16321 cagaagccat tcatatggca acctatgtgc cagctgtttc tgtcggtatt gacgacgtat
    16381 gtggtcaaat caaagctggg catgcggctg actttattgt tcttgataag gatttaacac
    16441 ttgttgcaac ctatttaaat gggcaaaaag cttttgatgc ctaagagata caaaaggatt
    16501 ggggtagcta tgtctggcag gccaattctt tttgtatttt ctttctttat gatagaatag
    16561 attacagtaa tcgcttacag agattgataa aaggaggttt ttgtgatggt aacaacagta
    16621 aaaatgacaa gtggctatga gattcccgtt ttaggatttg gaacttacca agcagccgat
    16681 ggtgaggaag cttaccaatc taccttagca gccatcaagg ctggctatcg tcatattgat
    16741 actgcagcta tttataaaaa tgaagaaagt gtgggtcgtg ccatcaagga ttctggggtt
    16801 ctacgagaag acttatttat tacaactaaa ctctggaatg acgcccatag ttatgaaggg
    16861 gcaaaagacg ctttagctgc ttctcttgac catcttggtc tggattatgt ggatctctat
    16921 cttatccatt ggccaaaccc aaaagctctg cgtaacactt ggaaagaggc aaatgctcaa
    16981 gcatggcaat atatggaaga ggcggttgaa gcaggactca ttaaaagcat tggcgtcagc
    17041 aattttatgg tgcatcatct agaagctctt caagagacag ctaaaataac tccagcaatt
    17101 aatcagattc gattagcccc aggttgctat caaaaggaag tggttgatta ttgtaaggca
    17161 aatgagattt tattggaagc ttggagtcct ctaggacaag gggagatttt tgataatgag
    17221 actatgcagc agttagctaa taaatatgac aaaacagtgg cacaagttgc cttagcttgg
    17281 tcgctggcag aaggttttat tcccttgcca aaatcggtgc atgatgagcg catcaaggaa
    17341 aatatggcta tttttgatgt gagcttgaca caagaagata aaaaaacaat tcgctatctc
    17401 tcaggaatgt cagcgactcc taaccctgat actacaagtt tttaggaaag tacaaagatt
    17461 tagccataat tcgctaaatc ttttttgaaa acggtttgac aaaggtttat catttagcta
    17521 taatctttat tagtgttttg tgaaagaaac tattattgaa aataaggagc cgtattgtga
    17581 ccatcttttt agtgatgtta gccatgttta ctattcgttt agtatttttg aagaaatcga
    17641 taacaaatga aaaagccata ttagcccaag gaggacagga atttggtgct cagaacacga
    17701 aatttttgac gttacttcac attatgattt atgtgtttgc ggtgattgaa gcgctgctta
    17761 aacaaattaa gtttgatggc atcagttttt taggtctttt gttgatgtta ttatcagtag
    17821 ctgttttata tgaagtgaca cgtatcttag gtgacatttg gactgtaaaa ttgatgttag
    17881 caaaagacca caaatatgtg gatcactggt tgttcaaaac gattaaacat cctaactact
    17941 ttttaaatat cgcccctgag ctggtgggca tagccttact ttgccatgct aaaattacgg
    18001 ctatgcttct tttcccatgc tacattgtag taatatattt acgtattcga gaagaaaaca
    18061 agctattagc ggaagtgatt attccaaatg gtactcgaac aaaaccttaa gctcatgatg
    18121 gacaaactaa tggctttatg ctaaactaga ctctaggaaa aaggatgcaa gtatcttatc
    18181 taaagcgagt tcggggtagt tagagccgaa tggtaggact gcagattggc gcttccgttt
    18241 gggcagtgtg attaagtata tttgtcaata ttgcccaaaa agatactata taaatgaagt
    18301 aataaattag ggtggaaccg cgttttgacg cccctaggtc ttatgattta ggagcatcaa
    18361 aatgcggttt tttggtgcct agacctgtaa tacatgctag taacatcgtc aggtatttta
    18421 ctgatgacaa aaaaacgatt gtcaatgata taccatttta ctactatcaa aggagtacga
    18481 aatgtctaaa aaacttacct ttcaagaaat catcttgacc ttacaacaat attggaatga
    18541 tcagggttgt atgctcatgc aagcctatga caatgaaaaa ggagctggga ccatgagtcc
    18601 ttacacgttc ttacgtgcta taggtcctga gccatggaat gcggcttatg tagagccatc
    18661 acgtcgtcct gcagatggcc gttacggaga aaacccaaat cgtctttacc aacaccacca
    18721 atttcaagtg gtcatgaaac catcaccatc aaatattcaa gaactttacc ttgcttcttt
    18781 ggaaaaattg ggcatcaatc ctttagaaca tgatattcgt tttgttgaag ataactggga
    18841 aaacccatca acaggatcag cagggcttgg ttgggaagtt tggcttgatg gaatggaaat
    18901 cacacaattt acgtatttcc aacaagttgg tggccttgcg acatctccag tgacagcaga
    18961 agtaacttat ggtcttgagc gcttagcttc ctatattcaa gaagtggatt cggtctatga
    19021 tattgagtgg gcaccaggtg ttaaatatgg tgaaatcttc ttacaacctg agtatgagca
    19081 ttcaaaatat tccttcgaaa tatcagacca agacatgctg cttgaaaact ttgaaaagtt
    19141 tgaaaaagaa gcctcgcgtg ccctagaaga aggtttggtt cacccagctt atgattacgt
    19201 gctcaaatgt tctcatacgt ttaacctgct ggatgcccgt ggcgctgttt ccgtcacaga
    19261 acgtgcaggc tacattgctc gtatccgtaa tttggcacgt gtcgttgcta agacctttgt
    19321 tgcagaacgc aagaagcttg gatttccact gttggatgaa gcaacacgcg caatcttgct
    19381 agctgaagat gacgaataag tcgatttaaa gatgatctag ttgcgagctt tgtaatctta
    19441 agagcgagca agaagtgggc gaccgaaggt cgccgaatca aggttactcc cgctgtagtg
    19501 acaattcggt aaggttactt atcgaacgag ggagttggcg agactttagg ctggctaact
    19561 aggaatggaa cacttttgtt ttctgaaatt atccactgga taatttcact ttgcgtcccc
    19621 cctacctaag ggaacaatca aaaagatact ttttagagaa aggaactgaa cacgggctac
    19681 ggactatgtc aaaaagacca agacttccta ggtgcaagca cctgcgtcgc tttgctattt
    19741 tgatggtgtc cgcttaacgc ctttggtatc ttaattatga gtaaaaatct actaatcgaa
    19801 cttggtctgg aagaacttcc agcctacgtg gtcacaccaa gtgaaaaaca actgggagag
    19861 cgcttggcga ctttcttaac agaaaatcgt ctctcttttg aagatataca aaccttttca
    19921 actccacgcc gtttggcagt gcgtgtgtct ggattagctg atcagcaaac ggatttgact
    19981 gaggacttta aagggcctgc taagaaaatt gcccttgatg ctgatgggaa tttctcaaaa
    20041 gctgctcaag gatttgttcg tggcaaaggc ttgaccacag atgctatcga attccgcgaa
    20101 gtcaaaggag aagaatacgt ttatgtgacc aaacacgaag ctggaaaacc tgctaaggaa
    20161 gtgcttctag gcgttactga agtgttgtca gcaatgacct tcccagtcag catgcactgg
    20221 gctaacaata gttttgaata cattcgtcct gctcacacct tgactgtact cttggatgat
    20281 gaagctctgg accttgactt cttggatatt cattctggac gcgttagccg tggccaccgt
    20341 ttccttggaa aagagacgac catcaccagt gcagactctt atgaggatga tttgcgcagc
    20401 cagtttgtta tcgctgatgc taaagagcgt caagagatga ttgtggagca aatcaagacg
    20461 ctggaagtgg agcagggtgt gcaagttgac attgacgaag acttgcttaa tgaagttttg
    20521 aacttggtcg agtttccaac agctttcatg ggaaattttg aggctaaata ccttgatgtc
    20581 ccagaagaag tattggtaac atcaatgaaa aaccaccaac gttattttgt ggtacgtgat
    20641 caggctggtc acttgatgcc aaactttgta tcagttcgta acgggaatga tcaagccatt
    20701 gaaaatgtta ttaaagggaa cgaaaaagtt ttggtagctc gcttagaaga tggtgaattc
    20761 ttctggcgtg aggatcagaa attgcaaatt gctgatttag ttgccaaatt gaccaatgtg
    20821 accttccatg aaaaaatcgg ctctcttgct gaacacatgg atcgtacgcg tgtcattgcc
    20881 gcatcacttg ctaaggaagc caacctatct gctgaggaag tcacagctgt tgaccgtgct
    20941 gcccaaattt acaaatttga ccttttaaca ggtatggttg gtgaatttga tgagctgcaa
    21001 gggattatgg gtgaaaaata tgcccgtcta gctggtgaag atgctgctgt ggcaagggct
    21061 atccgagaac actacttacc tgatgctgca ggaggtgcgc tacctgaaac caaagttggc
    21121 gctgttctgg ccttggcaga taaattggat actctcttgt cattcttttc tgttggcttg
    21181 attccatctg gctctaatga tccttatgct ctacgtcgtg caacgcaggg tatcgttcgt
    21241 attcttgatc attttggatg gcgcatccca atggataagc tggtagatag cttatatgac
    21301 ttgtcctttg atagtttgac ctatgccaac aaggcggatg tgatgaactt tatccgtgct
    21361 cgtgtggata agatgatggg caaagccgca ccaaaagaca ttcgggaagc tattctagca
    21421 agctcaacct ttgtggttcc agaaatgtta gcggtagctg aagctcttgt caaagccagc
    21481 catacggaaa attacaaacc agcagttgaa tctttgtcac gtgcttttaa tttggcagaa
    21541 aaagcggacg cttctgttca ggtggaccct agcctctttg aaaatgagca agaaaacact
    21601 ttgtttgcag ttattcaagg cttgactcta gcaggcagtg ctgcccagca gttagaacag
    21661 gtctttgctc ttagcccagt gattaatgat ttctttgata acacaatggt tatggcagaa
    21721 gatcaagccc ttaagaacaa ccgtttagct attttatcag atttagtaag caaagctaag
    21781 accattgctg cttttaacca actaaacacc aagtaaatag aaaggaaaga gccgttgggg
    21841 acttagctag aaaagtgagt tcagtacccg agtggaaaaa acaaggcctt taaacattgc
    21901 ttttcacagt gagagttaag tcagtttttt aagccttata cttttttccc aggctcttag
    21961 tggcttaaca tggatcctaa aaaaattgct cgtatcaatg agctggctaa aaagaaaaaa
    22021 acggtaggct tgactggacc tgaaaaagtg gaacaggcaa aactccgtga agaatacatt
    22081 gaaggctatc gtcgctctgt cagacaccat atcgaaggca tcaaattggt ggacgaagaa
    22141 ggaaacgacg tgacccctga aaaattaaga caggtgcaac gtgaaaaagg cttgcacggc
    22201 cgttcattag atgacccaaa atcataagac aaaaagcagc tctgaactgc tttttgttta
    22261 tcgtgatgac tgttagcttt tattttaaat ggtatgatac ttcaaagtga ccacacgttt
    22321 ttatattgta tagtcaaaat attaggaaaa accatgtgtt tgagttataa tagaagaaat
    22381 caaagaaaga aggtagtact tatgacaatt aaaaaattct ctctactagc catagccagc
    22441 ctctctttac tcagtcttgc agcctgtgat atggatgata aagacgatca tatggataat
    22501 caaccaaaga caagtcaaac tagcaagaaa gttaaacttt cagaagacaa agccaaatca
    22561 attgcgttaa aagatgcttc tgttacggaa gctgatgctc aaatgctatc agttacacaa
    22621 gataatgaag atggcaaagc cgtgtatgaa atcgaatttc aaaacaaaga tcaagaatac
    22681 agttatacca ttgacgccaa ctcaggggat attgtggaaa aatcttctga accaatcaat
    22741 gactaatcaa gaactgtaat gaataagaca gccttaactc ttgtgcccaa ttgtctttgc
    22801 ctcttaggca cggctgtttg ataccgtgtt gcctttgagg cttagttctc tttatgaccc
    22861 aatgatgact ctggattaga agagtgattg ttgggtcttt tgattttctt tgataagaaa
    22921 gattacctcg ttagaaatga ggagacgtgg ttgtcatcag tggatttttc aagatcatct
    22981 agtgggataa gctacctatc ttatcagctt ttttttagct ttaaaaattc ttgataatat
    23041 atttctgata aaattagcta taatagaatc aaataggaaa acgcttactt aaaggagggt
    23101 aatgaatgtc gcaagaaaag tatatcatgg ctattgatca gggaacaaca agttcacgag
    23161 cgattatttt caatcaaaaa ggggaaaagg ttagcagtag ccaaaaagaa ttccctcaga
    23221 ttttcccaca cgctggttgg gtagagcaca atgctaatca gatttggaat tcggttcagt
    23281 ctgttattgc gggtgctttt attgagtcca gcatcaaacc aagccaaatt gaagcaatcg
    23341 gaattaccaa ccaacgtgag accacagtgg tttgggacaa aaaaacgggt gtacctattt
    23401 ataatgctat tgtgtggcaa tcacggcaga cggcaccaat tgctgagcaa ctcaaacaag
    23461 atggccatac aaaaatgatc catgaaaaga caggccttgt tattgatgct tacttttctg
    23521 caaccaagat ccgttggatt ttggaccatg tgccaggtgc tcaagaacga gctgaaaaag
    23581 gcgagttact ttttggaacc attgatactt ggctggtttg gaaattgaca gatggtgctg
    23641 ttcatgtgac ggattattcc aatgcggctc gtaccatgct ctataatatt aaagacttaa
    23701 catgggatga tgagatttta gagcttttaa atattccaaa agatatgctt ccagaggtta
    23761 aatccaattc tgaaatttat ggcaaaacag ctgcctttca tttttatggg ggcgaagtgc
    23821 ctatttcagg tatggcgggt gaccaacagg cagctttgtt tggccaacta gcttttgagc
    23881 ctggtatggt gaaaaacact tatggcaccg gttctttcat tatcatgaat actggtgatg
    23941 agatgcagtt atcgagtaat aatcttttga ccaccattgg ttacgggatt aatgggaagg
    24001 ttcactatgc cttggaaggt tccatcttta ttgcaggctc tgccattcaa tggttgcgag
    24061 atggtcttaa aatgattgag acttcaccag aatctgaaca gttcgctcta gcatcaacta
    24121 gtgacgatga agtttatgtt gtgcctgctt ttaccggttt aggagctcct tactgggatt
    24181 ctaatgcgcg tggatctgtc tttggcttga cacgtggtac cagcaaagaa gactttgtca
    24241 aagcaacctt acaatctatc gcctatcaag tgcgagatgt tattgatacc atgcaggtgg
    24301 attcaggtat tgacattcaa cagctccgtg tggatggcgg tgcagccatg aataatatgc
    24361 tcatgcaatt tcaggctgat attttaggaa ttgatattgc ccgtgctaaa aatttagaaa
    24421 caacagctct aggagcagct ttcttagcag ggcttgctgt gggctactgg gaagacatgg
    24481 atgctttgaa agagttaaat gccacaggcc agctctttaa ggctagcatg aatgaatcgc
    24541 gcaaagaaaa attatacaag ggttggaagc gcgctgttaa agcaactcaa gttttcaccc
    24601 aagaagaaga tgcagatgac gatgcaaaat aactagagga ggatggaatg gaattttcaa
    24661 aagagacaag acgcttagct ctccaaaaaa tgcaagaaag agatttagac ctactgatta
    24721 ttgggggagg tattacgggt gctggtgtgg cgcttcaggc ggcagctagt ggcctagata
    24781 cgggtctgat tgagatgcaa gattttgcgg aaggaacctc cagtcggtcg accaaattgg
    24841 ttcatggggg acttcgttac ttgaaacagt ttgacgttga agtcgtctca gatactgttt
    24901 cagaacgtgc tgtggtccaa cagattgcac ctcatattcc taagccagat cctatgttat
    24961 tacctgttta tgacgaacct ggcagcacct ttagcatgtt ccgtttgaag gtcgctatgg
    25021 atttgtatga tcttttagca ggcgtgtcca atacgccagc ggccaacaag gtgttaacca
    25081 aagaagaagt cttaaaacga gaaccagact taaaacaaga aggcctgctt ggtggtggcg
    25141 tttaccttga tttccgcaat aatgatgcaa ggcttgttat tgaaaatatc aaacgagcta
    25201 atcgtgatgg ggctcttatt gctagtcatg tgaaagcaga agatttcttg ctagatgaca
    25261 atggtaagat tattggtgtg aaggcgcgtg atttgctgtc agatcaagaa atcattatca
    25321 aggctaaatt agtcatcaac accacaggtc catggagtga tgagattcgt caattctctc
    25381 ataagggaca accgattcat caaatgcgcc ctacaaaagg ggtgcatctg gtagtggacc
    25441 gtcaaaaatt accagtgtct caacctgtct atgttgacac agggttaaat gatggtcgta
    25501 tggtctttgt cttgccacgt gaagaaaaaa cttattttgg aacaacggac acggactaca
    25561 ctggagactt ggagcaccca caagttactc aagaagatgt ggattatttg ttaggcgttg
    25621 tcaataaccg ctttccaaat gccaacgtga ccattgatga tattgaaagc agttgggctg
    25681 gtcttcgccc cttgttatca ggtaatagtg cttctgacta caatggtggc aacagcggta
    25741 aagtcagtga tgatagtttt gatcacttgg ttgatactgt caaagcctat attaaccacg
    25801 aagatagccg agaagctgtt gaaaaagcta ttaagcaggt tgaaaccagc acatctgaaa
    25861 aagaattgga tccgtctgca gtgtcacgag gttcaagttt tgaccgtgat gagaatggac
    25921 tctttacctt ggcaggtggt aagattaccg actatcgcaa aatggctgaa ggagcattga
    25981 cagggattat tcaaatcctc aaagaagagt ttggcaaatc cttcaagctt atcaattcaa
    26041 aaacctatcc tgtttcagga ggtgaaatca atccagccaa tgtagattcg gaaatagaag
    26101 cctatgctca attaggaact cttagtggtc tttcgatgga tgatgctagg tatttggcaa
    26161 acctttatgg ttctaatgcg ccaaaagtct ttgccttaac tcgtcaatta acagcagctg
    26221 aagggttaag tttagctgaa accttgtcct tacattatgc gatggattat gaaatggctc
    26281 ttaaaccgac agattatttc ttgagaagaa caaatcacct cttatttatg cgagatagcc
    26341 tagatgcttt gattgaccca gtgattaatg aaatggctaa acattttgaa tggtctgatc
    26401 aggaaagagt ggcacaagaa gacgatcttc gtcgcgtgat tgcagacaat gatttgagtg
    26461 ccctaaaagg ccatcaggag ggttaatatg gatatctttg gtgaattttt gggtacagtc
    26521 ttattggttt tactaggaaa tggtgtggta gcaggggttg tcttgcccaa aacgaaaacc
    26581 catgcttctg gctggatcgt gattgcgact ggctggggaa ttgcagttgc tgtggcagtc
    26641 tttatcagtg gcaaagttgc ccctgcctac ctcaatccag ctgttagcct tgcttttgcc
    26701 atgagtggaa ccattgcctg gtcaacagcc attgcctata gccttgccca actattggga
    26761 gctatggttg gttcaactct ggtattcctt cagttcaggc cacattatct ggctgctgag
    26821 agtcaggctg atattttagg gacatttgcg acaggtcctg ctattcgaga tactagctca
    26881 aacctattga gtgaaatctt tgggaccttt gtcttgatgc ttggtatttt ggcatttggc
    26941 ttatatgata tgccagcagg actaggaacc ctctgtgtag gtaccttggt tattgggatt
    27001 gggttatcct taggaggaac gacaggttac gccattaacc cagcgcgtga tttaggacct
    27061 cgtctagttc atgctattct accactcaac aacaagggag attctgattg gtcttatgct
    27121 tggatacctg ttgtaggacc aattattgga gcggccttag cagttttgct tttccaagtc
    27181 atgtcctaac catcactaaa ggggttggct atcataggcc agctcctttg cattttcttt
    27241 ataggattag gtaaaacgct ttcatatctt aaaaacaact gctataataa tactattatg
    27301 acattaacga caaagaccat acatgttatc ggggcatcct ttgctggtct agcctttgtt
    27361 gataaatata aggacctcaa tccagattcc caaatcattc tcattgataa ggaaagctgt
    27421 cccaattata ttccaaatgg tatcaaccaa ttattcagag gagacattca agacctgtct
    27481 gatgctatgt ggggaagagc ctgcctagca gcacaaatag aatctaacca ccggattatt
    27541 caagcagaag tgttagctat cgaagctccc tcaaacacct tgcttctcaa agacagtcaa
    27601 ggacgagttt ttgaagaagg ttatgaaacg cttgtttgtg ccatgggtgc tagcccccag
    27661 tctcattata ttgagacgtc acagaccaac aaagttttgg taaccaaata ttatgaagaa
    27721 agccaagcta gtttaaaact gattgaggct agccaagagg tcttggttat tggtgccggc
    27781 ttgattggct tagaccttgc ctacagtttg tcattacaag gcaaacgggt caaactgatt
    27841 gaagcagccg aacgtccaga tttctatcag actgacgcag aactgatagc ccctgtaatg
    27901 gcagaaatgt ccactcacca tgtcaccttt attaataata agcgtgttac ggctattcat
    27961 gagatagagg gaaaagttgt cgctcatacc gagcaagggg acaccttcca aggtgatttg
    28021 gctattttag ccattaactt taggccaaat acacaccttt tgcaaggaca agtggcttgt
    28081 gctctggata agaccatttt ggttaatgaa aacttacaga ccagccaggt taatatctat
    28141 gccattggag acatggtttc attgcatttt ggtatattgg gaatggatta ctatacacct
    28201 ttgattaacc aagcaatgaa gacaggtcag gctctggctc tgcatttagc tggatatcct
    28261 attcctccat tacagacggt caaggtatta ggaagttctc actttggtta ttatcgcgcg
    28321 agtgttggtg tgacagaaga agaagcagag ctttatatgg atacgtgtag ctatttgtac
    28381 caaaatgggg attctaaaaa cctcttttgg ctgaaattaa tagctagaaa aacggatggg
    28441 attttaatag gcgctcagct cctatccaaa accaatgctc ttgtcatcgc caatcaatta
    28501 ggtcaggctc tggctctgaa agttacagac gctgacttag catttcaaga ttttctcttt
    28561 ttacaaggtc acagtgattt ggcttatcat cttcatgaag cttgtctcaa attatttgaa
    28621 aagaggctcc gccatgaaga ttgaagactt aatggataag gaacgaaggg cccaatatcg
    28681 tctcttggtg accctttatc atgctaaaga aacgttaagg ctaaaagacc taatgaggct
    28741 atcaaacttg tctaaagtga ccctattaaa atacattgac aatctaaatc atttgtgtcg
    28801 agaacaaggt ttagcctgtc aattactttt agaaaaagac agtctgagct taaaagaaaa
    28861 tggccagttc cattgggagg atttggtagc tctgcttctc aaagaatcgg ttgcctatca
    28921 gatattgacc tacatgtact gccataaaca ctttaacatc accaacttat ccgttgaact
    28981 gatggttagt gaagctactc taaacagaca attagctcac ctcaaccagc ttttatcaga
    29041 atttgatctg gcgctctcgc aaggcagaca actgggaagt gagcttcagt ggcgttattt
    29101 ctattttgaa ttgttccgcc ataccttgac cagacaaggc atagacgcct tggtcaatca
    29161 gttagacgct tcgcatttgg ctaccttaat tgagcggctg attggccagt cattatcggc
    29221 agaagcctta gaacaattat tgatttggtt ggcgatttcg caagcgcgaa tgtcctttca
    29281 aaaaggctac aatgatcatt ttttaagaga tagtgacttt atgacatcta atattttctt
    29341 taagcgctta gaaagcatgc ttttacatta cttaagacgc tatgcccttg aatttgatgc
    29401 ttttgaagct aagagcctct ttgtcttttt gcatgcttat cctctacttc ctattgccag
    29461 catgaagtat agtttgggtt ttggaggtcc tattgctgat cacatttcag aagccttatg
    29521 gcttttaaag aaggctcatg tgattatcca tcagacaaag gaggaaatca tttatggctt
    29581 ggggatattt ttttccaaag cttacttttt taaaggagtt atccttagtc aaccaaccaa
    29641 tagccaatac ctttatcaat tagtaggcga agataaaaga gcgcttttaa gggttatcat
    29701 aaaccattta gtgctgcaaa tggatcagga aacggatttc agtcagcagt tgagtgacga
    29761 cattttagca ttgttgattt tttccattga gcgccaccat gagcctcttt tagtagggct
    29821 tgccttgggg caaaataagg tggaagcagc cattgcggaa ttggccatta gacgtcattt
    29881 ggggcatcgt agggattttc aattaatgcc ctatgatcat cagaaagttt atgattgtct
    29941 gattacctat cagactgtct gtttaccgag acaagattta ccctattacc ggttaaaaca
    30001 gtatagctcc ccctatgaac tcactgcttt agaagctttt ctcaaagacc ttttccagca
    30061 aaaaaataca cgagaagagg aactctcact cagcccagca gctaagtcta gttttgcaca
    30121 caaaactgtg tgatacttgg tcttttctaa ggactgttac acttttataa taataaagta
    30181 ggatagtcta aaggtagatg atttgaaaaa gcttatcaaa tagagcttaa caaaatcgaa
    30241 aatttttgga ggtaagaaat gaaatttttc ctagatacag ctaatgtagc cgctattaaa
    30301 gcgattaatg aacttggcgt tgtagatggt gtcaccacca atccaaccat tatctcacgt
    30361 gaaggacgtg attttgaaac agttatcaaa gaaatctgtg acatcgtcga tggcccaatc
    30421 agtgctgaag tgacagggtt aacagcagat gctatggtag aagaagctag aagcattgct
    30481 aagtggcatg acaatgtcgt cgttaaaatt ccaatgacta cagaagggtt aaaagcaaca
    30541 aacattcttt caaaagaagg gattaaaact aatgtgacct tgattttcac agtaagccaa
    30601 ggtttaatgg caatgaaagc aggcgcaact tacattagcc catttatcgg tcgtttggaa
    30661 gatattggca cagatgctta tcaattaatc agcgaccttc gtgaaatcat tgacttgtac
    30721 gatttccaag cagaaatcat cgcagcaagt attcgtacaa cagcccatgt ggaagctgtt
    30781 gctaaattag gcgcacacat tgcgaccatt ccagatccct tgtttgctaa gatgatgcaa
    30841 catcccttaa cgacaaatgg tttgaaaacc tttatggaag attgggcttc ctttaaaaaa
    30901 taaggctaaa gagtctgtgg ctactgtgtc aacaggctct cttatattca tcgtcaaaaa
    30961 gaattcacca atgatgtcca aactagtttt tttctggcaa aatagggaaa aagaatttag
    31021 ggactttgga ggattttctg aaaaaagcgt ctatttttgt gatacaatag acaatagaaa
    31081 acgtttaatt ttagttgtaa taaacagaga ggttttactt atgacatttg atgcaattga
    31141 ccaattggca gtaaacactg tccgcaccct atcaatggat gccattcaag cggcaaattc
    31201 tggacaccca ggacttccaa tgggggcagc acctatggcc tatgttcttt ggaatcactt
    31261 catgaacatc aatcccaaaa caagccgtaa ttggtcaaac agagaccgtt ttatcctatc
    31321 agcaggtcat ggaagtgcca tgctttatag cttgttgcat ttagcaggtt atgatttatc
    31381 tgtagaagat ttaaagaact tccgtcaatg gggttctaaa acaccaggtc acccagaagt
    31441 gaaccacaca gacggtgtcg aagcaaccac aggacctctt ggtcaaggga tcgcaaatgc
    31501 cgttggtatg gccatggcag aagctcacct agcagctaaa tttaacaaac caggctttga
    31561 catcgttgat cactacacat ttgctttgaa tggtgacggt gaccttatgg aaggggtcag
    31621 ccaagaagca gcaagtatgg caggacattt aaaacttggg aaattggtct tgctatatga
    31681 ttcaaacgac atctctcttg atggtccaac ctctatggct ttcacagaag atgtgaaagg
    31741 acgtttcgaa gcttacggtt ggcaacatat ccttgtgaaa gatggaaatg atttagaaga
    31801 aattgcagct gctattgaag cagctaaagc tgaaactgag aaaccaacca tcatcgaagt
    31861 caaaaccatt attggttttg gtgccgaaaa acaaggaact tcagctgttc acggcgctcc
    31921 tcttggagca gaaggcattg cttttgccaa aaaagcttac caatggactc atcaagattt
    31981 tgaagtgcct gctgaagtaa cagagcgctt tgctcaaggt cttcaagcgc gtggtgaaaa
    32041 agcagaacaa gcctggaatg acttgtttgc agcttatgaa gcagaatatc cagaattagc
    32101 agcagaatac caaaaagcct ttactaacga agctgctcaa gtagaacttg aagcgcacga
    32161 acttggtagt tcaatggcga gtcgtgtatc cagtcagcaa gctatccaac aaatttcaga
    32221 acaagtagcc tctttctggg gtgggtcagc agacctttca gcttcaaaca atactatggt
    32281 taaggctgaa acagatttcc aaccaggtca ctacgaaggc cgtaacatct ggtttggcgt
    32341 tcgtgaattt gccatggctg cagccatgaa cggtattgcc cttcacggcg ggacacgcgt
    32401 ttacggcgga accttctttg tcttctcaaa ctatcttctt ccagctgttc gtatggcagc
    32461 gctccaaaac ttgccaacag tttatgtcat gacacatgat tctatcgctg tcggtgaaga
    32521 tgggccaacg cacgaaccaa ttgaacaatt ggcaagtgtt cgctcaatgc ctaacttgaa
    32581 cgttattcgt ccagcagacg gtaacgaaac aaatgctgct tggaaacgtg ccattgctga
    32641 aacagatcgc ccaaccatgc ttgtattgac gcgtcaaaac cttccagtgc ttgagggcac
    32701 aaaagaattg gcagaagatg gtcttaacaa aggagcttat atcttgtcag aagccaaagg
    32761 cgaccttgat ggtatcatca ttgcaacagg ttcagaagtg aaactggcaa tggatactca
    32821 agaagctctt gaagcagaag acattcatgt gcgtgtcgta tctatgccat cacaaaatat
    32881 ctttgacgaa cagtcagcag agtataaaga aagcatccta ccagcagctg tgactaaacg
    32941 tctcgctatc gaagcaggtt caagctttgg ctgggcaaaa tatgtcggcc tagcaggaaa
    33001 aacattgacc attgacactt ggggtgcttc agctccagga aatcgtatct tcgaagaata
    33061 tggtttcact gtggcaaatg caactgagtt atacaaatca ctctaggtta cacatcccaa
    33121 acagagtaaa tcatttgatt tactctgttt ttttaaaata aaatactcag ttctttcttt
    33181 tcctcaaact gcctaataca cttcttttga taagaagtca ttgacaaaac gattcataaa
    33241 aattataatt taattgttat tgtattagcg taaaagtaca aaaaataaag gagtataaat
    33301 ggaaaagaaa cgacagcgct tatacgatgt gattagacag gcttatgatt atccagaaaa
    33361 ccgtgaaaat gtggccttgt cgcagctact tttagcagct tctaatagat taattaaaca
    33421 cagtacccca cttcttgttg cctaccaact aaaccaagat gtggataatt acttacttga
    33481 taatgacatt ttacttccaa aatcgctttg ccgttttaag cagtcattgg aaaagtatat
    33541 tttagaggaa gcctcatcag tctagggaga aaaagagaag aaaaggaaaa aggataccat
    33601 gatagaattt aaacatgttt caaagttgta tggcgacaaa gaagctctca gcgatttaaa
    33661 cgtcaccatc aatgacggtg aaatttttgg ccttatcgga cataatggtg caggtaaaac
    33721 cacgaccatc agcattttga cttctattat cgaagcgagt tatggcgaag tatttgtgga
    33781 tggtcaactt ctgacagaaa atagagaagc tatcaaaaaa caaattgctt atgtgccaga
    33841 ttctcctgat attttcttga atctaactcc aaatgagtat tggcaatttt tagccaaaat
    33901 ttatggggtg tcagatgagg accgagaaga acggttagca cagctgacaa cactttttga
    33961 actgaaggaa gaagttaatc aaaccattga tagtttctca catgggatgc gccaaaaagt
    34021 gattgtgatt ggtgctcttg tatcaaatcc taacatctgg attttggatg aaccattaac
    34081 aggtttggat cctcaggctt cttttgacct taaagaaatg atgaaagctc atgcagcttc
    34141 tggacatacg gtcttatttt cgactcacgt gctatcagtc gctgagcagt tatgtgatcg
    34201 tatcgggatt ctaaaaaaag ggaaattgat ttttgttgga acgattgatg agttgaaaga
    34261 acaccatcct gacaaagact tggagagcat ctaccttgaa ctcgctgggc gtaaggcgca
    34321 agaagagggg tgatgggatg aattggtcta ctatttggga acttatcaag ataaacattc
    34381 tttattccaa tccacaaagt ttagctaacc tcaaaaagcg tcaggaaaaa cacccgaaag
    34441 aaaacttcaa ggcctataaa agcatgatga ggcaacaggc cttaatgatt gccatatttt
    34501 tggtgattta cctttttatg tttattggtg ttgatttcag tcattatcca ggacttttct
    34561 cctttgacgt tgctatgttt tttatcatgt caaccttgac agcctttagc tctctttaca
    34621 ccatttttta tgaaagtaat gacctaaaac tttatattca cttaccagta acgtcagaag
    34681 aactctatat tgcaaaaatt gtctcatcgc tagggatggg tactgtcttt ttgatgcccc
    34741 ttatctctct tctcttaatt gcctattggc aactcttggg aaatcctttg tctattttag
    34801 tagctatcgt gctcttttta gtgttactag tgagttctat ggtattagct atttatatca
    34861 atgcttgggt aggcaaaatc attgtaagaa gtcgcaaacg aaaactcatt tcaactatca
    34921 tgatgtttgt ctcaactttt ggcgctttcg tcttaatttt tgcgattaat attagtaata
    34981 acaagcgtac gatgacggat ggcgtattta ctgattaccc aactattccc tatttcaaag
    35041 gattctatga tgttgttcag gcaccgtttt ctactgcggc cctccttaac ttttggttgc
    35101 cattgttact tatcctagct atggtatatg gtattgtcac aaaagtgatg ccaacttatt
    35161 atcgtgaggc tttttatatt agtaacgaga acaaagtcaa gcaaactaaa aaaccagtta
    35221 atcgtcctca tcagaatcaa tcactggcgc agttgttgcg aaaacatcac ctattaacgt
    35281 tacaaaatgc aactttactg acacaaacct atctcatgcc cctgatgtat gtgatgcttt
    35341 ttatcggtcc aagtttgtca cgtggcacag gtttctttaa gcatatttcc ccagattact
    35401 ttggggtggc cttattattt ggggtcagtt tgggtgtcat gtgtgcaaca ccaaccagct
    35461 ttattggagt aggtatttca cttgaaaagg ataactttac ctttattaaa agcttaccaa
    35521 taacgttaaa aaaattcttg ctggataaat tttgcttgct tgtaggtctg caactgattg
    35581 tgcctatggt gatttatctt gtgtttggtc tctttgtgtt gcacctgcat cctcttctaa
    35641 cgattgcttt ttgtcttggc tacgccctgt ctctgattgt gcaaggggag tttatgtatc
    35701 gtcgtgacta ccggcttctg gatttaaaat ggcaagacat gacacaactc tttaccagag
    35761 gaaatgggca gtggctaaca atgggcctta tctttggtaa tttaatagtc acaggtgtag
    35821 taggttttgg agctgttatc attgccaata ttatccaaca acctctgttg attagtgttc
    35881 tactgagttg tctgacacta atggttttag gtcttgcgca attatggatt caaaaaacct
    35941 tctggaaaag cttagagagg ctttaacgaa gagggatcat tattcggaaa aacagtcctc
    36001 tggggctgtt ttttatgcta taatttgggt atgatgaaac gacaatttga agatgtaaca
    36061 cgtatcgtaa ttaaaattgg cactagttca ttagtgctac caacaggtaa aatcaattta
    36121 gaaaaaatcg accaactggc ttttgtgatt tcaagtttga tgaataaggg caaggaagtt
    36181 attttagtat catcgggagc gatgggattt ggactagata ttttgaaaat ggaaaaacgt
    36241 ccgaccaatc ttgctaaaca acaggcagtt tccagtgttg gtcaggtggc catgatgagt
    36301 ctttattcac aaatttttgc acactaccaa accaacgtgt cgcaaatttt attgacgaga
    36361 gatgttgtgg tttttccaga aagtctagcc aatgttacca atgcttttga aagtctgatt
    36421 agtttgggca ttgtgccgat tgttaatgaa aacgatgcgg ttagtgtgga tgaaatggat
    36481 catgcgacta aatttggaga taatgaccgt ttatctgctg tcgttgctgg tattacaaaa
    36541 gctgatttat taatcatgtt gtctgatatt gatggccttt ttgataaaag ccctactatt
    36601 tatgaggatg cgcagctacg cagccatgtt gcggtcatta cgcaagaaat cattgcgtca
    36661 gcaggtggtg cgggcagtaa gtttggcaca ggtgggatgc ttagcaaaat tcagtctgct
    36721 cagatggtat ttgaaaacaa aggacagatg gtcttgatga atggagctaa tcccagagat
    36781 attttaagag tgcttgaagg gcaaccacta ggcacctggt tcaagcagat agaagaggta
    36841 acacatgact gatatgagaa ggttagggca aagggcaaaa caagctagtc ttttaatagc
    36901 acctttgtcg acgcaaatta aaaattgctt tttaagcacc ttagcaaagg cattagttga
    36961 cgatacacag actctcttag ccgctaacca aaaagattta gcgaatgcta aagaacatgg
    37021 tatttctgac atcatgatgg atcgcttgcg cttaacttca gaacggatta aagcgatggc
    37081 acagggagtt caacaggttg ctgacttagc tgatccaatc ggacaagtca ttaagggcta
    37141 tactaacctc gatggcctta aaatcctcca aaagagagtt cctctagggg tgattgcgat
    37201 gatttttgag agtcgcccga atgtttcagt ggatgctttt agtctggcgt tcaaaaccaa
    37261 taatgccatc atcctaaggg gaggaaaaga tgcctttcac tcaaataaag ctttggtgaa
    37321 gttgatccga caaagtctgg aaaaatcagg gatcacccca gatgctgtcc agctagttga
    37381 ggatccctca catgctgtcg ctgaagaatt gatgcaggca acggattatg tagatgtttt
    37441 gattccacgt ggtggtgcta aattaattca gaccgttaag gaaaaagcaa aagtgcctgt
    37501 catcgaaaca ggtgtgggga atgtgcatat ctatgtggat gctcaagctg acttagatat
    37561 ggctactaag attgttatca acgctaaaac aaagcgccca agtgtttgca atgcagctga
    37621 gggattggta attcacgaag ctgtagcagc aaggtttatt cctatgcttg aaaaagcgat
    37681 caatcaggtt cagccagtag aatggcgggc agatgacaag gctttgccgt tatttgagca
    37741 ggcagtgcct gcaaaagcag aggattttga gaccgaattt ttagattata tcatgtctgt
    37801 taaggttgtt tcctctttgg aagaagctat ttcttggata aatcagcata ctagtcacca
    37861 ttccgaagct atcatcacaa gggatattaa ggcggcagag acctttcaag atttagtgga
    37921 tgctgctgct gtttatgtca atgcttcaac acgatttacc gatggttttg tttttgggct
    37981 tggtgctgaa attgggattt ctactcaaaa aatgcacgcc cgtgggccaa tgggcttaga
    38041 agccttgaca agcactaaat tttatattaa tggggatgga catatcagag aataagctac
    38101 tagaaggtcc cttaatgact aatgaaagag aaaaagaaga agcaaaggtt gctttagaag
    38161 aacttttaca tcagattcag gtgattcatt tggaattgaa aattgtccga aattggggct
    38221 attttgtttt tttggagatg gtatgctggt cagctggatc aaacgtcgtg agcaagttta
    38281 accaattatt aatagactta caagttactc ttcaaaactg ccgaagatag ctggcagacg
    38341 tcgataggct attattagct gacttaccga atatgagttg ggataaaatc tttgatattt
    38401 ggttagacaa cctgctgaca gattgttgtg tgattaagga agctaaaaat cttttacaag
    38461 cagttgaaac tctagaggca gcggctaagt cagtgcttgc taagttggct gatagcgaac
    38521 aaaaagaaca agattagaga agaggaagat tcccacgaga atgaaatcct tttctcttac
    38581 tttttcattt ctaaaccttt tgaagtatgg tacaattaaa gttatgacaa aagaatttca
    38641 tcacgtgacc gtactccttc acgaaacagt ggacatgctt gacataaagc ctgatgggat
    38701 ttatgttgat gcgacgctag gtggctcagg gcactcagct tatttgttgt ccaaacttgg
    38761 tgaagaaggg cacctctatt gttttgacca agaccaaaag gctattgaca atgcacaagt
    38821 taccctccaa tcttatattg acaaaggaca ggtaactttt attaaagata attttagaca
    38881 cctcaaagca cgtttaacag cgcttggagt tgatgaaatt gatggtatct tatacgacct
    38941 tggtgtttcc agcccgcaat tggatgaaag agaacgaggg ttttcttata aacaagatgc
    39001 tccattggat atgcgcatgg atcgtcagtc gctcttaaca gcttacgaag tggtgaatac
    39061 ctatccattc aatgatttgg ttaagatttt tttcaaatat ggtgaagata aattctccaa
    39121 gcagatcgct cgaaaaattg aacaagcaag agctattaag cctattgaga caacaacaga
    39181 gttggcagaa ttgattaagg cagcaaagcc agctaaagag ttgaagaaaa aaggccatcc
    39241 tgctaaacag atttttcaag ctattcgcat tgaagtcaat gatgaattgg gagcggctga
    39301 tgaatctatt caggacgcta tggaattatt agcccttgat ggtcgtatct cagtcattac
    39361 cttccattct ctggaagatc gcctaaccaa gcagttgttt aaagaagcta gtacggtgga
    39421 tgtgccaaaa gggcttcctc taattcctga agatatgaaa ccgacatttg aacttgtttc
    39481 acgtaagccg atcttaccta gtcattcaga gttaacagct aataaaaggg cacactcagc
    39541 caagctacgt gttgccaaaa aaattcggaa ataagcctat gacaaatgaa aaaagaactc
    39601 aggcagttac aaatgccttg caaaaacgaa taaaaacttt ctctagaatt gagaaggctt
    39661 tttatactgc tatcattgta acagcgatta cgatggcagt aagtattatt tacctgcaaa
    39721 gtcgtaagtt gcagctacag caagaaatca ctagtttaaa tagtcatatt tcggatcaaa
    39781 aactagagct taataatgcc aagcaagaag taaatgagtt atctcgccgt gatcgtatta
    39841 tagatattgc tggtaaagca ggcttaagca accgtaataa taatatcaag aaagtcgagt
    39901 aatctatgaa aaaatggcaa aaatatgttt tagattatgt tgtgcgcgat aggagaactc
    39961 cagtcgaaaa tcgcgttcga gttggacaaa atatgatgct cttaactatc tttattttct
    40021 ttattttcat tattaatttc atgattatta ttggaacaga tcaaaagttt ggagttagtt
    40081 tgtcagaagg ggcgaagaag gtttatcaag aaaccgttac gatccaagct aagcgtggga
    40141 ccatttatga tcgaaatggt acagctattg cagtggattc tacgacttat agcatatacg
    40201 caattttgga taaatcattt gtctcggctt cagatgaaaa gttatatgta caaccttctc
    40261 agtatgaaac agtagctgat attttaaaaa agcatctggg aatgaaaaaa acagatgtaa
    40321 ttaaacagct taagcgtaaa ggacttttcc aagtctcgtt tggaccgtca gggtctggca
    40381 tttcatatag tactatgtct actattcaaa aggctatgga agatgccaaa atcaagggaa
    40441 ttgctttcac aactagtcct ggtcgtatgt atccaaatgg gacatttgct tcagaattta
    40501 taggcttagc atctctaaca gaagataaaa agacaggtgt taagagttta gttggaaaaa
    40561 caggtctaga agcttctttt gataaaattt tatcaggtca agatggtgtt attacttatc
    40621 aaaaagatcg aaatgggacc acactcttgg gtacaggtaa gactgtcaag aaagctattg
    40681 atggcaaaga tatttacaca acgctatctg agcctatcca gaccttctta gaaacccaga
    40741 tggatgtttt tcaagccaaa tcaaatggtc agttggccag tgcaacactt gttaatgcta
    40801 aaactggtga aattttggca acaacacaac gccccactta taatgctgat actctgaaag
    40861 gacttgaaaa tacgaactac aaatggtaca gtgcacttca tcaaggaaat tttgagccag
    40921 gctcaaccat gaaagtgatg actctggcag cggctattga tgataaagtt ttcaacccaa
    40981 acgaaacctt tagcaatgct aatggtttaa caattgcaga tgctactatt caagactggt
    41041 ctattaacga aggcatttcc acaggacagt acatgaatta tgcacaaggg tttgccttct
    41101 caagtaacgt tgggatgact aaacttgaac aaaaaatggg taatgcaaaa tggatgaatt
    41161 atttgacgaa gttccgcttt ggttttccta ctcgttttgg tttaaaagat gaagacgcag
    41221 gtatatttcc ttctgataat atcgtgactc aagctatgag cgcttttggt caagggattt
    41281 ctgtaaccca gattcaaatg cttagagctt ttactgctat ttctaataat ggtgagatgt
    41341 tagagccaca atttatcagt caaatttatg atcctaacac agcaagtttt agaacggcaa
    41401 ataaagaaat tgttggaaaa cctgtatcaa aaaaagccgc tagtgaaaca agacaataca
    41461 tgattggtgt aggaacagac cctgagtttg gaacactcta ttcaaaaaca tttggaccaa
    41521 ttattaaagt gggtgattta cctgttgctg ttaaatcagg aacagcacaa attggttcag
    41581 aagatggaac tggttatcaa gatggtggat tgactaacta tgtttattcg gttgtggcaa
    41641 tggtgccagc tgataaacca gactttttga tgtatgttac tatgactaaa ccacaacatt
    41701 ttggtcccct tttttggcaa gatgtggtta acccagtatt ggaagaagca tacttaatgc
    41761 aagatacact aactaagcca gtagtatcag atgctaatcg tcaaacaact tataaattac
    41821 caaactttgt aggaaagaat cctggtgaga catcaagcga gttgcgtcga aatcttgtcc
    41881 agccagttgt ccttggtact ggcagcaaga tcaaaaaagt atcgcatcag cccggtcaaa
    41941 cgttaacaga aaaccaacaa gttctcatat tatcagaccg ttttgtggag gtaccagaca
    42001 tgtatggctg gacaaaatcc aatgttaaaa cctttgctaa atggactggg atagacatca
    42061 gctttaaagg aacagattct ggtcgtgtta tgaaacaaag tgttgatgtt ggtaagtcct
    42121 tgaaaaaaat aaaaaaaatg accattactt taggagatta acatgttttt aacccttatt
    42181 gcagcaatca tatcctttat ggtatctgct ttcacaatgc cttattttat caaattctat
    42241 caattgaaaa aaattggtgg gcaacaaatg catgaagatg tcaagcaaca tttagctaaa
    42301 gctggaacac cgactatggg gggaactgtt tttcttttag tagctacggc agtatcgtta
    42361 ttggttagtc tattttcgat caaaaacact caaagtttag ctttgatttc aggcatttta
    42421 tcaattgttg tcatttacgg gattatcgga tttttggatg attttttaaa aatttttaag
    42481 caaatcaatg aaggattgac agccaaacaa aaattagcct tgcagttggc tggaggattg
    42541 atgttctatt ttttacatgt tagtccaagt ggcatttctt cgatcaatgt gtttggttat
    42601 cagctgtcgc tgggaatttt ctacctattc tttgttctat tctgggtggt tggtttctca
    42661 aatgccgtta atttgacgga tggtatcgat ggcttagctt ctatctcagt ggtgattagt
    42721 ttagtgactt acggcgttat tgcctacgtt cagagtcaat ttgatgtttt gttactgatt
    42781 ggaaccatga ttggtgcttt acttggtttc ttcctattta accacaagcc tgccaaagta
    42841 tttatgggag atgtaggtag tttagccctt ggagctatgt tggcagctat ttctattgcg
    42901 cttcgtcaag aatggacctt actcattatc ggaattgtct acgttcttga aacaagttct
    42961 gtgatgttgc aagtgtccta tttcaagtac accaagaaaa aatatggaga aggtcgtcgt
    43021 atttttagaa tgacgccttt ccaccaccat ttggaattgg gaggcttgtc tggtaaaggc
    43081 aagaagtggt cagaatggca ggtagatgct tttttgtggg gagtaggaag tctagctagt
    43141 ttactagttt tagctatctt atacgtcttc taagggggat tatcaagaat agtcaccaat
    43201 agcattttga ttaacatcat caataagaga gtggagatga cccacaaata ctgattttgt
    43261 gagtgttgtc ccactccttt tgcttgtctc taacaataat gatataatat ggaagaaaac
    43321 gaggtaaaac atgtcattta aagattatca ttttaaacaa tacatccagc aagcactgga
    43381 agaaattggt tttgtcaatc caacagaggt ccagaagcgg ctgattccta ttgttaactc
    43441 cggtcgtgac ttagttggcg aatcaaaaac aggttcaggg aaaacccaca cctttttgtt
    43501 gcccattttt gaaaaacttg atgaaactaa agcagaagtg caagtcgtta ttacagcacc
    43561 aagccgtgaa ttagcaactc aaatttttga tgcttgtaaa cagattgcta agcacttcca
    43621 agaagagatt cgtttggcca actatgttgg aggcacagac aaactccgtc agattgaaaa
    43681 actaaaaggc agccagccgc atattgtcat cggaacccca ggtcgtattt atgacttggt
    43741 caagtctggt gatttagcga ttcataaagc aacgaccttt gttgtggatg aggccgatat
    43801 gaccatggac atgggctttt tggatacggt agataagatt gcagcttcgc ttccaaaatc
    43861 tgttcagatt ttggtgttct cagcaactat tccacagaaa ttacaaccct ttttgaaaaa
    43921 atacttgacc aatcctgtta ttgaacaaat caagacaaag accgttatcg cagataccat
    43981 tgataactgg cttgtgtcga caaaagggcg tgataagaat ggtcaactgt tagagattct
    44041 caaaaccatg cagccttata tggctatgct ttttgtcaat accaaggaac gcgcagatga
    44101 tttgcatgcc tttttaacag ctaatggctt aaaagtggct aagattcatg ggggcattcc
    44161 tccaagagaa cgtaagcgca tcatgaacca agtcaaaaaa ctggactttg aatacattgt
    44221 tgccacagac ttagcagcgc gtgggattga cattgaaggg gtcagccatg tgatcaatga
    44281 cgccatccca caagacttgt ctttctttgt ccatcgtgta ggccgaacag gtcgaaatgg
    44341 tatggcagga actgccatta cactttacca gccaagtgac gactcagata tcaaagagct
    44401 ggaaaaaatg gggattgcct ttacaccgaa ggtgctcaaa aatggagaat ttcaagatac
    44461 ctatgaccgt gatcgccgtc aaaaccgtga aaaagcctat caaaaattag acacagaaat
    44521 gattggtttg gtgaagaaga aaaagaaaaa agtcaaacca ggttacaaga aaaaaattca
    44581 gtgggcggtt gatgaaaaac gtcgcaaaga acgtcgtgct gaaaatcgtg ctaagggccg
    44641 cgctgagcgt aaggccaaaa aacaacattt ttaagaacaa agagcaagca agtgatcaat
    44701 tggcatctaa tcttgctttt tttacacctg ctacttgtat tgtttaccat ttgttaacac
    44761 aaaaagtgat catagctttt gcttattgct ataatacgga tttactattt tacatgtggg
    44821 tctaagagtg ttagaatatt aagacaaaca aatacaaagg ggaaatacat gacatcagtg
    44881 tttttaacca gtggttgggc tttttatgat cacctcatct caccgattcc acatgggaaa
    44941 ctttttagtt ggcatgctgt ttttgatgcc attccaaata ttatccaacg gcttccaatt
    45001 acgcttggtt tgacactatc aggagcaacc tttggcttgg ttttggcttt gatttttgcc
    45061 cttgtcaaaa ttaataaagt gaagctacta taccctattc aagctatttt tgtgagtttc
    45121 ttgcgaggaa ctcctatttt ggtacagttg atgttgacct actacggtat tcccctcttt
    45181 ctaaaatgtc tcaatcagaa gtatggcttt gattggaatg ttaatgcaat tccggcttct
    45241 atttttgcca ttacagcctt tgcttttaat gaagcagcct acgctagtga aactattcgg
    45301 gcagccattt tatcagtaga tacaggtgaa attgaagcag caaaaagtct aggcatgact
    45361 tctgtgcagg tttaccgtcg tgtcattatt cctaatgcaa ctgttgtggc tattccaacc
    45421 ttgattaatg ggttaattgg cttaactaaa ggaacatcgc ttgcctttaa tgcagggatt
    45481 gtcgaaatgt ttgctcaggc tcaaattcta ggcggatcag attaccgtta ttttgaacgt
    45541 tatatctctg ttgctcttgt ctattggtct atcagtattt tgatggagca agtaggtcgc
    45601 ttgattgaaa acaagatggc catcaaagca ccagaacaag ctagaaatga aaagttagga
    45661 gaattgcgtt gatgattaca attagaaatt tgagcaagac attctcaggg caaaaagttt
    45721 tagatagttt ggctcttgat attgaaaaag gtcaagtgat tgctttagtt ggtgcttcag
    45781 gagcagggaa atcgaccttc cttcgtagcc taaactattt ggaaaaacca gattccggct
    45841 ccattagcat tggtgacttt acggttgatt ttgagaccat taccacagag caagtcctta
    45901 ttttacgtcg gaaacttgcc atggtttttc aacaatttaa tctttttgag cgtcgtacag
    45961 cccttgagaa tgtcaaagaa ggtcttaagg tggtcaaaaa actgtctgac caagaagcga
    46021 ctaaactggc gcaagcagaa ttggctaagg ttggtttagc agatcgaaaa catcattacc
    46081 cacgtcattt atcaggggga caaaaacaac gggtagcttt agcaagagct ctggctatga
    46141 aaccagatgt tttattacta gatgagccga cctcggctct tgacccggaa ttagttggcg
    46201 aagtggaaaa gtctattact gatgctgcta aatccggaca gaccatggtt ttagtgagtc
    46261 acgacatgaa ttttgtttac caagtggcag atagagtact ctttttagat caagggaaaa
    46321 tcttggaaca aggaacacct gaagaagtct ttagacatcc tcaaaaagaa cgaaccaaag
    46381 aattttttgc aagctattca aaaacataca tttaatgtta ttcttttgtt ataataaaaa
    46441 ggtctaggta ttcctgggct tttcttgtta tgttttttag aaaaacttat gggaaagaaa
    46501 taatgactag gtggaagtat gttagtagaa atctttagcc tttatgtaaa aggtctcatt
    46561 ctctcaagcc ttactatgat tttaatttgt ttaatatgga ctttatggcg agcaaaaacc
    46621 aaaagagata aaacattgat tgaaagacaa gcttttcttt atgagatgct aatggttgct
    46681 attttaacca tcccaatttt atcgtttgct tttatgagta ttttggttgt cataaaatcc
    46741 taatcattgc aagaagggat tatcattgct aaaatattgg caagagtaga ttaaaggaga
    46801 aaaatcatgt acgatacatt aattattggt tcaggaccag caggaatgac tgccgcttta
    46861 tacgctgcta gaagtaattt atctgttgcc attattgaac aaggggcacc tggtggtcag
    46921 atgaacaaca cttttgatat agagaattac ccgggatatg atcatatatc tggaccagaa
    46981 ttagctatga aaatgtacga acctttagag aagtttaacg ttgaaaacat ttatggtatt
    47041 gttcaaaaaa tagaaaattt tggtgactat aagtgtgtgt tgactgaaga tgctagttat
    47101 gaagctaaaa cggttattat tgcaacggga gctaaatacc gtgtactagg tgttccaggt
    47161 gaagaatact atactagtcg aggagtttct tattgtgcag tatgtgatgg tgcgtttttc
    47221 cgtgaccagg atcttttggt cgttggtggt ggtgactcag cagttgaaga agctatttat
    47281 cttactcagt ttgctaaaaa ggtaaccgtt gttcatcgcc gtgatcaact aagagctcaa
    47341 aaaatactgc aagatcgtgc ttttgccaat gataaggtcg actttatctg ggattcagtc
    47401 gtcaaagaaa ttcaaggcaa tgatattaag gtctccaatg tcctaatcga aaatgtgaaa
    47461 acaggccaag tcactgacca cgcttttgga ggtgtgttta tctatgttgg tatgaatcct
    47521 gtgacaggta tggtaaagga tctagaaatt accgattcag aaggttggat tatcacagat
    47581 gatcatatga ggacaagtat tcctggtatt tttgcgattg gagatgttcg tcaaaaagac
    47641 cttcgccaaa tcacgacagc tgttggagat ggtgcaattg cagggcaagg tgtttatcac
    47701 taccttgaaa gtttcccttc ataaggttaa aactccttca tttctccttg tctttccctt
    47761 aaaaaagcta aattgcataa gacctttaag aaatataaag aacagttctc cttaatatct
    47821 ttcctagata aatgttgaaa attttctgaa aaaaggatat aataaatagt attttagtga
    47881 atggagttta gaatgtctat aaaagaacaa acagacaata acgaactaga aaatggaatg
    47941 gtaaggggct tagaaaatcg tcatgtgcag ttaattgcga ttgcaggaac gattggaaca
    48001 ggtctctttt taggggcagg acgctctatt gccttaacag gaccatctat tatttttgtg
    48061 tatatgatta caggtgcttt catgtttatg atgatgcgtg ccatcggtga aatgctctat
    48121 tatgatccag atcaacacac ctttatcaat tttatttcca aatacattgg tccaggctgg
    48181 ggttattttt cgggtctatc ctattggatt tcccttattt ttattggaat ggcagaaatc
    48241 acagcagtag gtgcttatgt gcaattttgg ttcccaagct ggccagcctg gttgattcag
    48301 ttggttttct tagtcttact tagttcgatc aacttaattg ctgtgcgcgt ttttggggaa
    48361 acagagtttt ggtttgccat gattaagatt ttagctattt tagctttgat tgcaacagct
    48421 attttcatgg tattgacagg ttttgaaact cacacaggcc atgctagcct ttccaatatt
    48481 tttgaccatt tttccatgtt cccaaatggg aaactaaagt tctttatggc cttccaaatg
    48541 gttttctttg cttatcaagc tatcgaattt gtggggatta ccacttctga gacggctaac
    48601 ccaagaaaag ttttaccaaa ggctattcaa gaaattccaa cccgtattgt gatcttttat
    48661 gtgggagcct tggtctctat tatggcaatt gtgccatggc atcagttacc agttgatgaa
    48721 tctccttttg tgatggtgtt caaattgatt ggtattaaat gggcagcagc cttgattaac
    48781 tttgtggtct tgacatcagc agcgtcagcg cttaactcaa ccctttattc aaccggtcgt
    48841 catctctatc agattgctaa tgagactcca aacgctttga ccaatcgctt aaagattaat
    48901 actttatctc gacaaggggt gccaagtcgt gccattattg cttcagcggt tgtggttggt
    48961 atctcagctt tgattaatat cctaccagga gttgcagatg cgttctcact cattacggcc
    49021 tcctcatcag gtgtatacat tgctatctac gccttaacca tgattgctca ctggaagtac
    49081 cgtcaatcta aggactttat ggcagatggt tatttgatgc caaaatataa agtgacaacg
    49141 cctttgaccc ttgctttctt tgcttttgtc tttatctctc ttttcttaca agaatccacc
    49201 tatatcggcg ccattggagc aacgatttgg attattgttt ttgggattta tagcaatgtc
    49261 aaatttaaat aataattagc gatatagaac tgaatccatt aactctttct tatttgagga
    49321 tgtcatgatg gatcagttct ttttttactt tagcgataac ttaagttatt tctaaatcct
    49381 agccaaagag tcctttttaa aatatgttat aatagtaaca ataattcaaa ggagtcccct
    49441 ttatgtataa agatgatagt ttaactctcc atactgattt gtatcagatt aatatgatgc
    49501 aagtttattt tgagcagggc attcataatc gtcatgcggt gtttgaagtc tatttccgca
    49561 aggaaccttt taacaatggt tatgctgttt ttgccggttt acagcgcatg gtggagtacc
    49621 tagagcagtt tcaattttca gaaacagatt tggcttacct tgaagaactg ggttatccag
    49681 aaaacttttt gacctacctt aaagagttac gattagaatt gaccatccgt tcagctaaag
    49741 aaggagattt ggtttttgct aatgagccga ttgtgcaagt agaaggccct ctaggtcagt
    49801 gtcagttggt cgaaacagct cttttaaaca ttgttaactt ccagaccttg attgctacaa
    49861 aggctgctcg tattcgttct gtgattgagg atgaacccct tttggaattt ggcacccgtc
    49921 gtgcacagga actagatgct gcgatttggg gaacccgcgc agcgattatt ggtggagcag
    49981 atgcaacaag taatgtgaga gcaggtaaac gctttggtat ccctgtttca ggtacgcatg
    50041 ctcatgcttt agtgcaagcc tatggtaatg attatgatgc tttcatggct tatgctaaga
    50101 cccacaaaga ttgtgtgttt cttgttgata cctatgacac gcttaaagta ggtgtgccaa
    50161 cagctatccg agtagccaaa gaaatgggtg acaagattaa ttttctaggt gttcgattgg
    50221 actctggtga cttggcttac ctctcaaaaa cagtccgtca gcagttagac gacgctggtt
    50281 ttaccgaggc taagatctat gcttctaatg acctcgatga aaataccatc ctcaacttaa
    50341 aaatgcaaaa ggccaaaatt gatgtttggg gtgttgggac caaactgatc acagcctatg
    50401 atcagcctgc cttaggtgct gtttataaga ttgtctctat tgagcaagaa gatggtagca
    50461 tgcgtgatac gatcaaatta tcaaataatg cagaaaaagt atccacacca ggtaaaaaac
    50521 aagtttggcg catcaccagt cgtgaaaaag gcaaatcaga aggcgactat atcacgttta
    50581 ctgatattaa tgttaatgaa ttaactgaga ttgagatgtt ccatccgacc tatacctata
    50641 ttaagaaaac cgtcagaaat tttgatgcaa ttcctctctt agttgatatt tttgtcaagg
    50701 gagaattagt ttaccagcta ccgaccttat ctgaaatcaa ggcttatgct aaaaaagaat
    50761 ttgacaagct ttgggatgaa tacaagcgtg tcttaaatcc tcaagactac ccagtagact
    50821 tggcacgaga tgtttggcaa aataagatgg ccttaattga taacattcgt aaagatactc
    50881 atggaaagag tgatttaaaa tgaccttaca agaagagatt atccgacaat taggcgtcaa
    50941 ggctagcatt gacccacaag aagaaattcg gaaaacagtt gattttttaa aagcttatct
    51001 gcgtaagcac tcatttttga aaacttatgt cttaggcatt tctggaggtc aggattctac
    51061 cctcgcagga aagctagccc agatggccat tgcagagtta cgggaggaaa caagtgatca
    51121 agcttaccaa tttatagctg ttcgcttgcc ttacggggtt caggctgatg aagcagatgc
    51181 acaaaaggcc ttggctttta ttgcaccaga tcagaccctt accattaata tcaaggcagc
    51241 tgtggacggt caagcagtag ccttgcaaca agctggcatc gagatttctg atttcaataa
    51301 aggtaatatt aaagcacgtc agcgaatgat aagtcaatat gccattgctg gccaaatggc
    51361 tggtgctgtt attggaacgg atcatgcggc agaaaacatc actggtttct ttactaaatt
    51421 tggtgatggt ggagcagata ttcttcccct ctttaggctt aacaaacgtc aaggaaaggc
    51481 tcttttgaaa gttttaggag cagatgctgc tttgtatgaa aaagttccaa ctgctgattt
    51541 agaagatcaa aaaccaggct tggctgatga ggttgctcta gaggtgacct atcaagacat
    51601 cgacgactat ttggaaggga agctgatttc caaagtagct caagcaacta ttgaaaaatg
    51661 gtggcataaa ggacaacaca agcgtcacct gccaatcacc atttttgatg atttttggaa
    51721 ataagagatg atgttcaaag agtaaaaaaa gaaaacaaaa aagttaggtt atcctaactt
    51781 ttttcttgaa gaaaatcaaa taaggtctat aatagaagta atcctttaga atagaatggc
    51841 tggataataa ccatcttgcc aatattattt caggtgaagc ttatcttaat attttaggag
    51901 gacaataatg tcagcattaa cagagacatt tacagaacaa ctatttgctc attacgaagc
    51961 cagtgcaaaa ttttcagcta ttgagaatgc cgttacgcat aatggtttgc taaaatcatt
    52021 agaaacccgt caaagtgaag tggacaatga ttttgtgttt tctattgatt taactaaaga
    52081 taaggtatct aaccaaaagg cttctggtcg ttgctggatg tttgctgcct tgaacacttt
    52141 ccgacacaaa ttaatcacag agtttaaatt agaaaatttt gaactgtcac aagctcatac
    52201 tttcttttgg gataagtatg aaaaagctaa ctggtttatg gagcaggtca ttgctacagc
    52261 tgaccaagaa ttaaccagtc gtaaagttaa gtttctcttg gatgttccac aacaagatgg
    52321 tggtcagtgg gacatggtag tttccttgtt tgagaagtat ggagtggtgc caaaatctgt
    52381 ttacccagaa tcaatctcat caagtaacag tcgtgaatta aaccagtatt tgaataaatt
    52441 attgcgccaa gatgctcaaa ttcttcgtga cctgattgcg tcaggcgcta aggcagacca
    52501 agttgaggat cgaaaagcag agcttcttca ggaaattttt aacttcttgg ccatgaccct
    52561 tggtttacca cctcgtcatt ttgattttgc ctaccgtgat aaagatgacc actatcatgt
    52621 tgagaaaggt ttgacaccgc aagcgtttta cgacaaattt gttggcttaa agctttctga
    52681 ttatgtgtct gttattaatg cgccaactgc tgataagcct tatggcaaat catacactgt
    52741 tgagatgcta ggaaatgtgg tcggtagtcg tgaggttcgc tacctcaacc ttgatatgaa
    52801 acgtttcaaa gaactagcta tcaagcagat gcaggctggt gagtccgttt ggtttggctc
    52861 tgatgtgggt caagtttcag atcgtcaaaa aggcattttg gcaactaata cttacgattt
    52921 tgaagctagt atggatatca atcttagtca agataaggct ggacgtttgg attatagtga
    52981 aagtttgatg acacatgcta tggttttaac aggtgttgac ttagatgaaa caggaaaacc
    53041 attaaaatgg aaggttgaaa attcatgggg tgagaaggtt ggtgacaaag gttactttgt
    53101 tgcttcagat gcttggatgg atgaatatac ctatcaaatt gttgtccgta aagaattctt
    53161 aacagctgac gagttagcgg cttatgaaaa agagccacaa gtactagctc cctgggatcc
    53221 aatgggtgct ttagcacaat actaattcct ttctttgcga ctgtatgaaa tctaaaagct
    53281 ggacaaaacg aacagcaaac ggattggact ccgcataaga aagtgaacat gaaaaaaatt
    53341 atgatgctaa gaaaatgaca tatgaaaaaa cacgtcttcg gtatatggaa acgtgttttt
    53401 ttcatgtata ctctcttatg gcattagttg ctatttataa caattttaaa taagtctatt
    53461 ttattttagg ttaacgtttg aaaaatcagt tgctaattcc cttaacgttg taccttttct
    53521 ttaatacgat taaagaattc tgttttttgt tttatattgt atgtggtagg tcatttgtgt
    53581 catataaaaa cgagtcctca cagaacgtgg actcgctttt tatttagatt atcacttaag
    53641 gtagatatta aaagtaagta aatggatatg taataaaagg taggtacaga caataagtag
    53701 agttagagat taatgagttg tctttttatc atcggtagat ggatgattgc tattgttgtc
    53761 tgtattggtt gaattacttg tatcattgct actactagta tcatctgaag tcgtttggct
    53821 agaagctgtt gtcgtgttat tgctgtaaag attgttgtaa accgaattag tatagtcggt
    53881 gttgctcgca taggttccgc ttaagtagag gaatccacca ctgcgataaa gaccatttgg
    53941 catcgtccaa tcttcactgt aaccatttgt taagtaagtc atcatgctac gataaacgtc
    54001 agatgcaatc tctagacttg atccgtagac tggggtcaag cggtttttgt aacctgtcca
    54061 aacagccatg gcgtagcgct tagtaaagcc aacaaagttt tcgtctggcg ctaatgtacc
    54121 aacataatct ggataaaggc cgtatttttc accaatttta gctagttcct catcagtgta
    54181 gttagaagtc cctgttttac cagcttgcgc aacaccagga atggcagcag cggtgcctgt
    54241 accataagtg agaacagttt ttaacatatc tgtcatcata taggccgtgg tttctttcat
    54301 ggcacgtttt cctttttcat caaaagtttt acttgtacca tcactaaatt ccactttatt
    54361 gacatacctt ggtttatgat aaataccacc atttgcaaaa gcagcgtatg cagcggccat
    54421 tttttcacta cttgcaccat attttttatc tgagctactg ttattacttg agatagcgtt
    54481 tgagtagtgc atttcgggat agttaatacc taaactgctt aagaaagatc gagcatagtc
    54541 aagtcctgcg gcttccagtg ctcggactgc tgggacattt cgcgatagca taatagcagc
    54601 ttggattgtc atccatccgt tatatctaag gtcccagtta aacaattggg tagtggttcc
    54661 aggccaataa tagactgagt cattagtcga ctgagcagta gaagtataaa caccagattc
    54721 aatagcagga gcataggctg tgattggctt catggtagaa ccccagtcac gatcagttaa
    54781 aacagcttgg ttagtcccaa atgaaacatt ttcatcttga ttacgtccgc caagctgagc
    54841 aataacatga ccatttgtca catcaacaat cgttgaagca acttggaaat cttggtctgg
    54901 atagtaaaca taatcaccag aatgataaat attataaaga gtctgctgcg catcggggat
    54961 aatattagta taaactttta aaccagcggt aaaaatatct ttatttgttt cttttttgac
    55021 ttcttcaata acttgtttta gataattatc catatatttt ggataagttg agcgctgttg
    55081 gagtgattgt agaccttcag cgacgggagt tgcgatggca gtttcatatt ctgctttcgt
    55141 cagatgtttt tccatgtaca tctgttgcaa cacgacgtta cggcgatttt gagcagcttc
    55201 aggatgaagg taaggatcat attgactagg agcttgaggg attccagcca atagggctag
    55261 ctgggcataa gataaatcct taagatcctt gccataataa gacttagcgg ctgtcagcat
    55321 accatagttg ccattaccca tatatacttt gttgatgtaa aaagtcagga tttcttgttt
    55381 agtatatttt cgctccattt gaagagcaag ccaaacttct tgagccttac gttttaaggt
    55441 ttgatcagat tcattagtag aaaaataggc tagtttaatg agttgctgat caagcgttga
    55501 ccccccttga gtggtctgac tcgttagatt atgaaaggca gcaccaaaaa tacgataaag
    55561 atctactcca cgatggttaa agaaacgttt atcttcaatt gaggtaatag cattaactag
    55621 attaataggg atactatcag ctgttacatt ttcacgcttt tcagaaccca aatcagcaat
    55681 cagattgtta ttaccgtcat aaaccaagct agagtttgtt gattttaact gggcttctga
    55741 cagtttcgga gcactgctaa tgtagaaggt aaacaaaaga ccaccaataa taataacaag
    55801 gataataagg ctaagaattg cacttaatac atactttagc cacttaagga tttttggatt
    55861 tttaattgta atcaccgcct aaaaaatttt gttcaattat gtttaaataa gggacttgtg
    55921 gaaaggctcc aaatgctacc ttaaagccat tttttctgat ataatcaatg ggcatcgatt
    55981 tactgccatt atctatctga taaaatgaaa tcaaggcttg agcaggtagg tagtaagtct
    56041 ccttcagtgt ggaaaagtga agaaggacaa agcagattcc tttttgatgt aaaacgcaag
    56101 ccatatgttc aatctgatgt aaatggaaat ttttcatggg catagctgtt ttttgcctgg
    56161 tttcttttgc ttcaaaatca acataatgtc ctttataaac gccgcaatag tcagtcgttg
    56221 aagcttgtct aaagtatgct tcaacgattt ttgcccgact gcgcttaggg taatcaactt
    56281 tgacaatttg gacaggagta ggttttttgt gaatgacagc tatttgacga gataaataat
    56341 aatcatttgt cgcgttgata gcagcttcaa aggacatacc tctattggcg aagtccacct
    56401 tattttgctt tgtttgcttt tgaacggaac ttactttttg tcgaataaga ttatgaggat
    56461 aattaaccat tgttctccta atattatcta gttaatgcta aaaatttaag cacttttatt
    56521 ataacatatt ttttgaaagg ataacaccta atgactgcga ttctaattac aggttaccga
    56581 agctttgaaa tagggatttt tgaccataaa gacccacgag tttcaattat taaacaggct
    56641 attcgtaaag atttaatagg ttatttggaa aacggtgtgg attggtttat ctttacaggg
    56701 aacttgggtt ttgaacaatg ggctttggaa gtagctaatg agctaaaaga ggagtatccc
    56761 ttgcagatag ctactatatt cttgtttgaa acgcatggag ataggtggaa tgaaaaaaat
    56821 caagaagttt taagtcagtt tagggctgtt gactttgtaa aatattactt tccaaattac
    56881 gaacaaccga cgcaatttag tcaatattat cagtttttat tagaaaagac agaaggggct
    56941 tatgtttttt atgacacaga aaacgagacc aacttaaaat attttctcaa gaaagcaaaa
    57001 gatatgccgc attatcagtt gctattgcta acctttgatc ggctaaatga tatgtcacaa
    57061 agctagcatt gtatttttag gggtattggt atttaaatac
//
LOCUS       DAUGFP010000008        49552 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.008, whole genome shotgun sequence.
ACCESSION   DAUGFP010000008 DAUGFP010000000
VERSION     DAUGFP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49552
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.008"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(69..368)
                     /locus_tag="VH663_000468"
     CDS             complement(69..368)
                     /locus_tag="VH663_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173696.1"
                     /translation="MFLTWENLFLTNQSRRRLKLLVVERSYNNVGNFLKEYFGGFFEI
                     VNFDDLRLTMVDMVSLSSEYDVIVTDMILEQTTDSEILFFNQMAPSVVANRLTDM"
     gene            complement(524..991)
                     /gene="perR"
                     /locus_tag="VH663_000469"
     CDS             complement(524..991)
                     /gene="perR"
                     /locus_tag="VH663_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEQ8173697.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICSDCQAKDQPDF"
     gene            complement(1043..1498)
                     /locus_tag="VH663_000470"
     CDS             complement(1043..1498)
                     /locus_tag="VH663_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEQ8173698.1"
                     /translation="MIYSFQNPSEDVLKAYLESAKTIAIVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEAQTILWSAGKEAIVMNRCLKIDYLQLVAKPECITNH"
     gene            complement(1676..4318)
                     /gene="polA"
                     /locus_tag="VH663_000471"
     CDS             complement(1676..4318)
                     /gene="polA"
                     /locus_tag="VH663_000471"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEQ8173699.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPEEFREQFPYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKASKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAAISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     REDIIGFAWGHGEQIYASTDLGLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKVADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQAKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            complement(4449..6176)
                     /locus_tag="VH663_000472"
     CDS             complement(4449..6176)
                     /locus_tag="VH663_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEQ8173700.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMAMTSGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMEAVMLDINKGMSPEAAAKKWVEAN
                     KSKVSSWTK"
     gene            complement(6192..7388)
                     /locus_tag="VH663_000473"
     CDS             complement(6192..7388)
                     /locus_tag="VH663_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ8173701.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            complement(7876..8967)
                     /gene="ulaG"
                     /locus_tag="VH663_000474"
     CDS             complement(7876..8967)
                     /gene="ulaG"
                     /locus_tag="VH663_000474"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEQ8173702.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(9220..10866)
                     /locus_tag="VH663_000475"
     CDS             complement(9220..10866)
                     /locus_tag="VH663_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEQ8173703.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INKALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            complement(11191..11895)
                     /locus_tag="VH663_000476"
     CDS             complement(11191..11895)
                     /locus_tag="VH663_000476"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEQ8173704.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVAKMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            complement(11897..12760)
                     /locus_tag="VH663_000477"
     CDS             complement(11897..12760)
                     /locus_tag="VH663_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEQ8173705.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPAIEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLA"
     gene            complement(12765..13427)
                     /locus_tag="VH663_000478"
     CDS             complement(12765..13427)
                     /locus_tag="VH663_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEQ8173706.1"
                     /translation="MTHIPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     MKEERGDRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIRRI
                     WG"
     gene            complement(13518..14003)
                     /locus_tag="VH663_000479"
     CDS             complement(13518..14003)
                     /locus_tag="VH663_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ8173707.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVRLAVQPLIDSKAVTSTYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLN
                     A"
     gene            complement(14126..14404)
                     /locus_tag="VH663_000480"
     CDS             complement(14126..14404)
                     /locus_tag="VH663_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ8173708.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            complement(14475..15908)
                     /locus_tag="VH663_000481"
     CDS             complement(14475..15908)
                     /locus_tag="VH663_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ8173709.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAISSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYFFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            complement(16215..18716)
                     /locus_tag="VH663_000482"
     CDS             complement(16215..18716)
                     /locus_tag="VH663_000482"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEQ8173710.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGKEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSGFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            complement(18928..20067)
                     /locus_tag="VH663_000483"
     CDS             complement(18928..20067)
                     /locus_tag="VH663_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEQ8173711.1"
                     /translation="MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELAQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            21013..21180
                     /locus_tag="VH663_000484"
     CDS             21013..21180
                     /locus_tag="VH663_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173712.1"
                     /translation="MKKKLSLFMIATAAVAGLSLATLGSHQTVSANDASYSNGFYGHT
                     GPDSWLLYTVW"
     gene            21590..21826
                     /locus_tag="VH663_000485"
     CDS             21590..21826
                     /locus_tag="VH663_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173713.1"
                     /translation="MKKKLSLFMIATAAVVSLSLATPENQTVSANDTQVTCVGCPDCG
                     PKWSCCPLTCMRDLLRGWGKSAAYYCTWGYYYSE"
     gene            complement(21922..22353)
                     /locus_tag="VH663_000486"
     CDS             complement(21922..22353)
                     /locus_tag="VH663_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173714.1"
                     /translation="MKQQSYQPLRFVYLLVALFAAMLLIARPVMADGENNDPANAAYY
                     KGKSAGEEAGKTAGKEASWTDLTPTNPTIPEPPSDIRETTNKQHYKDGYTDGYRDGYN
                     EGWKSQHPVLTPVKVIWDLISHWLQQFFPNNQATTTVQSMS"
     gene            complement(22608..24323)
                     /gene="slo"
                     /locus_tag="VH663_000487"
     CDS             complement(22608..24323)
                     /gene="slo"
                     /locus_tag="VH663_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEQ8173715.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKPESSELTTEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKELQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRS
                     EYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            complement(24347..24832)
                     /locus_tag="VH663_000488"
     CDS             complement(24347..24832)
                     /locus_tag="VH663_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEQ8173716.1"
                     /translation="MYKVPKSLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILEYGVYPAFIKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMVHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            complement(24837..26192)
                     /gene="nga"
                     /locus_tag="VH663_000489"
     CDS             complement(24837..26192)
                     /gene="nga"
                     /locus_tag="VH663_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEQ8173717.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFVSPM
                     RIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYHYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     KYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            complement(26563..27102)
                     /gene="nusG"
                     /locus_tag="VH663_000490"
     CDS             complement(26563..27102)
                     /gene="nusG"
                     /locus_tag="VH663_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEQ8173718.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            complement(27275..28318)
                     /locus_tag="VH663_000491"
     CDS             complement(27275..28318)
                     /locus_tag="VH663_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ8173719.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSSKQTTDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIETAK
                     KAIIEGTITVPEN"
     gene            complement(28645..29937)
                     /locus_tag="VH663_000492"
     CDS             complement(28645..29937)
                     /locus_tag="VH663_000492"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEQ8173720.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLDFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGERIDHYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            30375..31253
                     /locus_tag="VH663_000493"
     CDS             30375..31253
                     /locus_tag="VH663_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173721.1"
                     /translation="MIKKYDRTSTKKKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLVKAKIAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPQIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHDANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            31266..32528
                     /locus_tag="VH663_000494"
     CDS             31266..32528
                     /locus_tag="VH663_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEQ8173722.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDSM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIINANNAALQMLAETSKEAIPA
                     LEQSAQSPTLSMKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTHA"
     gene            complement(32648..33274)
                     /locus_tag="VH663_000495"
     CDS             complement(32648..33274)
                     /locus_tag="VH663_000495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEQ8173723.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYAIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(33319..34734)
                     /locus_tag="VH663_000496"
     CDS             complement(33319..34734)
                     /locus_tag="VH663_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEQ8173724.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGQPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGFYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            complement(34735..36510)
                     /locus_tag="VH663_000497"
     CDS             complement(34735..36510)
                     /locus_tag="VH663_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEQ8173725.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGDFTVTDTVYEIKQEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     ESNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFNEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            complement(36711..37031)
                     /locus_tag="VH663_000498"
     CDS             complement(36711..37031)
                     /locus_tag="VH663_000498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEQ8173726.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            complement(37028..38026)
                     /locus_tag="VH663_000499"
     CDS             complement(37028..38026)
                     /locus_tag="VH663_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEQ8173727.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTEIQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            complement(38042..38626)
                     /locus_tag="VH663_000500"
     CDS             complement(38042..38626)
                     /locus_tag="VH663_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEQ8173728.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            complement(38695..39174)
                     /locus_tag="VH663_000501"
     CDS             complement(38695..39174)
                     /locus_tag="VH663_000501"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEQ8173729.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            complement(39176..41176)
                     /locus_tag="VH663_000502"
     CDS             complement(39176..41176)
                     /locus_tag="VH663_000502"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEQ8173730.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEERHRQQAAQTALKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDHFYHQLKQHKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEADSVNQLKGLMTKTLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVAIWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            complement(41166..41486)
                     /locus_tag="VH663_000503"
     CDS             complement(41166..41486)
                     /locus_tag="VH663_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173731.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            complement(41940..42959)
                     /locus_tag="VH663_000504"
     CDS             complement(41940..42959)
                     /locus_tag="VH663_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEQ8173732.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            complement(42996..43361)
                     /locus_tag="VH663_000505"
     CDS             complement(42996..43361)
                     /locus_tag="VH663_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ8173733.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            43535..44215
                     /locus_tag="VH663_000506"
     CDS             43535..44215
                     /locus_tag="VH663_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ8173734.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVAKDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            complement(44337..44984)
                     /locus_tag="VH663_000507"
     CDS             complement(44337..44984)
                     /locus_tag="VH663_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ8173735.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(44987..45646)
                     /locus_tag="VH663_000508"
     CDS             complement(44987..45646)
                     /locus_tag="VH663_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ8173736.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLTLICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVALIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQAREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(45658..46842)
                     /locus_tag="VH663_000509"
     CDS             complement(45658..46842)
                     /locus_tag="VH663_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ8173737.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLIDQD
                     EYPKFGTTVDKLAKLRPAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            46963..47874
                     /locus_tag="VH663_000510"
     CDS             46963..47874
                     /locus_tag="VH663_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ8173738.1"
                     /translation="MNISSLAYFIEIVNCDFNLTKAAQKSHISQPALSSYIKTIEKEE
                     GVVLFQRQKGRLVGLTSVGERFFENAKVVIASHEQLMADLRSESKKYKGEISIGIPQL
                     ISGIVFSELIPKLIEENPEVKFHIVETGAYELQKKLFLKEVDFAILLTPTTLDATIAD
                     IDILLRDQLCLYMSHKTFQETFDSNPNISIKALHRQNLVTFDKTFMINHQLTTLFEEA
                     NSLPVVKCYSASWDFLLNCTRYSSYLTILPRPITHFAHMDGLVEVQLTEHPKWEVVLA
                     SLKHNKTSHLKHYIKHTILDYFMNQRF"
     gene            complement(47943..49349)
                     /locus_tag="VH663_000511"
     CDS             complement(47943..49349)
                     /locus_tag="VH663_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEQ8173739.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSLVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKGIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFLVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLYSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
BASE COUNT        14853 a        10732 c         9018 g        14949 t
ORIGIN      
        1 tattcttaat cttatttatc gctcattttt taaattccga tgctctcaca gatattatca
       61 ttgtttagtt acatgtctgt caatctattc gccaccacac taggggccat ctgattgaaa
      121 aataaaattt cggagtccgt tgtttgctca agaatcatat ctgtcacaat tacatcatac
      181 tctgaagaca gtgacaccat atcaaccatg gtcagcctca agtcatcaaa gttgacaatc
      241 tcaaaaaatc ctccaaaata ctcttttaag aagttgccaa cattgttata acttctctca
      301 accactaata gtttcaagcg acgacgcgat tgatttgtaa gaaagagatt ttcccaggtg
      361 aggaacaaac aatagaggag atgattgatg gttctttcct ttaattcctt atgatcccca
      421 gcaaataact ctttaacata agcaacaaag gtctggtagt gaactggggg tttttgattt
      481 gtaaaggcat tatatttata atgcctttat ttaagggtta actctaaaaa tcaggttggt
      541 cttttgcttg gcaatccgaa caaatcccgt aagcaataac tgggatacgc gtgaccttat
      601 aacctgtttg ctcatgggct tctttggcaa tatccataac atcaacatcc ataaagtcag
      661 caatcttccc acatatttca caaaccacat tgacgtgttg atggcccata aagtcatagt
      721 aggtggttag atcattgctg attttcaatt ctgagacaaa accttcatca actaaaactt
      781 tcaaattatt atagactgtg gcaaggctca tgttaggaaa gttaggctgt aagtcacggt
      841 aaatcttatc ggcactaggg tgttctgtgg actgaatcat gtaagaaata atggctttac
      901 gtgtctctgt aattcgaata tgtttttccc gtagatgctc taggacattt tcataggcat
      961 ctagggcttg ctgatgtgaa tgaatgtcca taagctgcta ctcctatcct tttttccttt
     1021 attatcacgc acgtctctag tttcaatgat ttgtgatgca ttctggttta gcgactaatt
     1081 gcaaatagtc tatttttagg cagcggttca tcacaatagc ctctttgcct gccgaccata
     1141 aaatggtttg tgcttcttgg ttttcaaggc ctaattgcgc ccaaaagact ttggcctgac
     1201 ctgctaaaaa gtctctggca acttctggta aaaactcact tctgcgaaag acatctacaa
     1261 tgtcaacctc aaagggaatt gccttgatac tggcatagac cttctctccc aaaataagct
     1321 gacctgctaa tttgggattg actggaatga tccgataatc catagcctgc ataaatttgg
     1381 cgacaccata agctgctgtg tctttgcggt cagaaagacc aacaatcgca atggttttgg
     1441 ctgactccaa gtaagctttt aggacatcct ctgagggatt ttgaaacgaa taaatcactt
     1501 gttcccctat ctattctaaa gctgttcaat ataggaggat aactcctaaa gcatggcttc
     1561 aatcactttt aaccttatcc gtcaaaaagc tcaaagcagc ttcatgactt ctcctacaat
     1621 tttttagttt attataacat aagttgtcaa aatgagaaat aacaagtaag aaaacttatt
     1681 tggcttcgta ccagctctgc ccagtacttt catcgacaca taaaggaaca gctaaatcaa
     1741 cagcagcttc catggtgtct ttgacaagtt tttttatagc agtcaactca tcatttggca
     1801 cttccaaaac gatttcatca tgcacttgaa gcagcatttt agctctaaaa ccacctgctt
     1861 gcaaggcctt atccagatta atcatagcga ttttgagaat atcagctgcg cttccttgaa
     1921 tgggcgagtt gatggctgta cgctccgcaa aagagcgaac gttgaaatta cgagagttga
     1981 tatccggtaa ttcacgacga cgcttaaaga gggtctctac atagcccttg tctttagcct
     2041 cacgcaccac attttccata taggctttaa tcccaggata gcgttcaaaa taggtatcaa
     2101 tgtaagactt ggcctgttta cgggtgatac caaggttatt tgacaaacca aaatcagaaa
     2161 tcccatacac aattccaaag ttaacagcct tggcattacg gcggtcattg gcagtaacat
     2221 ctgctacctt gtcaatccca aacacacgca tagccgttga ggtatgaatg tctgcccctt
     2281 cattaaaggc agcaatcaaa tgctcatccc ctgaaatatg tgccagtacc cgcaattcaa
     2341 tctgtgagta atctgaactc aacaagacgg catcttcatg agatggggta aaggccttac
     2401 gaatcaaacg cccctgttcc aaacgaatgg gaatgttttg aagattaggg tccacactag
     2461 acaaacgccc tgtctgggtg agatcctgca catagcgggt atgaatcttg ccatcagcca
     2521 gaatataatc ctgtaaacca atcacatagg tggattgcaa tttagtaatc tggcggtaat
     2581 ccaaaatttt agccacaatt ggagcaatcg gtgccaaacg ttccaaaaca tcaacagctg
     2641 tagaataacc tgttttggtc ttcttagtca tctcaagagg tagttgcatc ttctcaaaca
     2701 agatactgcc cagctgttta ggcgaattaa tgttaaattc ttggccagcc atatcataaa
     2761 tctcttgggt gagcgcttca ataatgacct tgttttgctc tgccatatct tgcaaagtcg
     2821 ccctattaac cttgatccct tcaatttcca ttttagcaag gacattagcc aaaggcaatt
     2881 caatattctg atagaggtct agttgctcat gggcagtgag cttgtctagc atttgagact
     2941 tgctatctaa taatactttg accttacgag ctaagtgttc tagtaagacg tctttatcag
     3001 gaacagcccg tttagcacct ttaccataga ccgtatcgtc ttcttccaaa gagatgtcag
     3061 taaaaatgcg ggcaatggtg ctaagctcat tgtcttctac agttgacaga aggtaattag
     3121 ctaatctagc atcatagcta ggtgctacca actcaattcc taagtggctc aacaagacct
     3181 tgctacgttt aaaatcataa gtggcaatgg gtttttggaa gacctgctta aaactatccg
     3241 tcgctaatag gccaaggtca gtggatgcgt aaatctgctc accatgtccc caagcaaaac
     3301 caatgatgtc ctcacgatgg taattatccc gaagcgtttc aaagtagaag atgtcttctg
     3361 cgctaaacaa atcagcagac acattggtca cttcttgata ggaaatggca gctactggct
     3421 cttgaggcat ctggctagca agacctttct tgagttgcac aaaatccatc tcatcgtaaa
     3481 attgagagag gctagcaaca tctggaccat tgtagacaat atcatctaag ccaattgtga
     3541 taggagatgc tgtattgata gtggcgagcg ttttagatag gaaagcttgg tcacggtcat
     3601 tgatcaagtt ttctttcatt tttgaggcct tgaatccatc aatatgctcg taaatgcctt
     3661 ccaaactacc aaattcatga aggagcttaa ggcctgtttt ttctccaatt tttgtcactc
     3721 caggaatgtt atccgattta tctcccatca aggctttgag gtcaataaac tggtttggcg
     3781 tcaagcccat tttttccatg aggtaagccg gtgtgaattc ttcaaattcg gcaacccctt
     3841 ttttagaaat ttcaaccacg gtattttcgt cagttaactg gatcaaatct ttatcaccac
     3901 tgacaatggt cacatcaaaa ggcacttctg tgcgctccgc cattttatca agcgtcccga
     3961 taatatcgtc tgcttcgtag tgttccaact cataataagc aatcccaagg gctgtcaaca
     4021 tctcacgaat ataaggaaac tgctcacgaa attcctctgg tgtcttggcg cgacccgcct
     4081 tataatcggc atacatttct gttcggaagg tggttttgcc cgcatcaaaa gccaccagca
     4141 cgtgagtagg ctggactctt ttcatcatgt ggtctagcat caaatgaaag ccataaatgg
     4201 cattggtatg aagaccacta tgatttttaa agcggtcaat ttgattgtag agggcaaaaa
     4261 aggcacgaaa ggcaacagat gagccatcaa ttaatagtaa tttattcttg ttttccatag
     4321 ccttattata acacagaggg acgggctttt ctcctttaag ccattggttt tgctgataag
     4381 taaatagtta acctaaaaag cctaggaaaa ttcctagact tctctatttg ctatttagac
     4441 aaaagcactt attttgtcca acttgatact ttagatttgt tagcctctac ccattttttg
     4501 gcggcagctt ctgggctcat gcctttattg atgtcaagca tgactgcttc catatctttt
     4561 tgggtccagt ggaacttatc aatgattttg tagacatttg gaagatcttt tttcaagcct
     4621 ttacgagcaa tggtattgat gttctcagtc gagccaaagg tttctttagg atctttaagg
     4681 tacttcaagt catacttggc aaacatccag tgtggtgacc aagctgtcac gacaatagga
     4741 tcttttttct tgatcgcttg atccaaggaa gtcgtcatgg ctcctgttga tgccgccact
     4801 aactcccaac tagatagatt atgatactct tttagagtct ttttggctgc tgccatgata
     4861 cctgcacctg gttcgatccc tgtgattttt tgatcggctt gtttgcttaa gtcctcaata
     4921 ctattgacat ctgtcatgta ttttggaaca gctaaaccaa gtttggtccc ttttaggtta
     4981 ggtcctaaat catctaattt tgacttgtat ttctgatatt gttggccatg ggtgactggc
     5041 aaccaagcac tagttgaaaa atcagcgttc ccatttgcaa cggtttgcca cataacggca
     5101 ttgtcaagcg gcgtcaaggt cacatggtaa ccttcgtttt tcaaaacttc cgcaatcaca
     5161 tgagttgagg caacctcaga atcccactgc acgtaagcga tgttaacagt ttcaccctta
     5221 tctgccatac cagaagtcat agccataata ccgcgtccta aagcagctat caaaaagaca
     5281 gcaagggctg ctagaccaat ccatttattg gttttgccag ctttggcttt ttcttgaggt
     5341 ttgctattga acaactgtgt catacggtca agcacaatcg ccaagataac caaggcaaga
     5401 ccactgacaa atccactacc aatatcagcg tgttgaaggg ctgacaagac ttcacgtcct
     5461 aaaccaggag caccaatcat ggagcctgtt actaccatgg aaagggctaa catcatggtc
     5521 tgattaacac ccgccatgat ggtattttta gctaaaggca attctacctt gaagagcttt
     5581 tgtttcccag ttgacccaaa ggcatctgag gcttcaatca attctgttgg aatgtcacga
     5641 atggccaaat ttgtaaaacg aactgttggg ggcaaggcaa agatgactga agcaaatact
     5701 ccaggcacca taccaatacc aaaaaaggca acggcaggaa tcaagtaaac aaaagccggc
     5761 atggtttgca taaagtccaa aataggattc acaatctgac ggacagtggc atttttagcc
     5821 atccaaatcc ccagcggaat accaataagt accgaaatca aactagctac taacaccaga
     5881 gtaaaggtat tcattagctg cttccataac ccttgattgt aaataaagag caatcctaac
     5941 aaggtaaagg ttggcaaagg ccattttttc ttagcaagga aaaacatccc tgctgttact
     6001 agaacaataa aaagtaacgg gtggataaat aaaagtgttt tggtcatcca atccatcaaa
     6061 aagctaccaa ctacttgcat aatatcaaat agacctgaga aagttttagt tagccactcc
     6121 gttagctgct ccaccagttg agctactggt aattttgttt gtaatatcgt ttccaaaatg
     6181 catcactcct tttagttaag gtcttcgtct gagatattag ccaaggcttc aatcactcgt
     6241 cctcgaataa tgacacctaa cagacgattg ttatcatctg tcactgcaat cggagcagat
     6301 gaatcataga taagaggcaa aatatctgta ataatagtat cttttgagac agttctcaca
     6361 tcgcgatcaa tcacttctga tagcggtaac ccttttttgc gcgcctctat agcggcatcg
     6421 gccgtcaaac taccgactaa ttggcggcgg cgattcgtcg ccatcaacat agacacctct
     6481 tcgttgtgca tacggttcaa ggcaacttga ggtccatcta attcaacagt agttgttaac
     6541 ggtttgatca tgatattttg tgctgtcaag actttagaac ggtccacatc ctcaacgaat
     6601 tcacgcacaa agtcattggc tgggttagtc aagatttcct caccagtacc aatttgcata
     6661 atttgtccgt ctttcatcaa agcaatccga tcaccaatcc gcaaggcttc attcaagtcg
     6721 tgactgataa agatgatggt ttgtttcatg ctatcttgca aatcaagtaa ttcatcttgc
     6781 atctcacgac gaatcaaagg atcaagcgct gagaatgcct cgtccattaa gagaatttta
     6841 gggctattag ctagcgcacg ggctaggccg acacgctgct gcatcccacc agatagttgg
     6901 tttgggtact ggtctttaaa atctaatagg cctgaattat caagggcttt ttctgccaat
     6961 cgctggcgtt cttctttggg aacaccacgt aattccaaac caaattcggt attttccaaa
     7021 atggttttat gaggaaagag ggcaaagctt tgaaaaacca tgttaatgtc atggcggcgc
     7081 acctcacgca gctgatctgc tgacatggtt gagatgtctt taccttccag caagatagat
     7141 cctgctgaag gttcaatcaa acgatttagc atgcggacaa gggttgattt cccacttcct
     7201 gatagcccca taataacaaa gatttcacct tttttgacct caaaactagc gtcatagaca
     7261 cctacagtag cgcctgtttt cttaaaaatc tcactcttat ttttgccagt ctttaccatc
     7321 tcaagagctg ctttttgttt tttgccaaaa attttactga gatgtttgac ttctaaaatt
     7381 gtttccattc gttttttctc ctaggtaatc gtttatcatg tgaaatagtg acatagcttt
     7441 ggcgaaatat tagctaatat ttaacaaagt agctctacac aattaataaa cgtgtggtca
     7501 cttttttact taaaaaaaga atctgccgaa acagaacata actatattat cacgtgtggt
     7561 cactttttgt caaggacaat aactcgatct ggtaatgcca caatgatagg tgatcgcttt
     7621 cattctttta gatatggtct tttaaccagt acaatttttt atcacaactg atgactattt
     7681 ctttgatgat taggctattg ttacattaga aatccttctt ttacctataa tatctatttt
     7741 gcggccatcg taaataacga ccacggtgac tcttaaacat ttgctcatct ctccaaaaaa
     7801 actcacctaa gacagtgtcc tatgaagcga gttagcttaa tcaggcacta tcatcttagg
     7861 tgagtagggt gttgcttata gtaaagcttt aaattggata ttagaatctt ctaagaagca
     7921 gtcatcaaat ccacgaggat gatggtattc gatacgattt tggtcttgtg gataggtgta
     7981 cttgccacca acctcccaga taaagggatg gaaatcgtat tggagacgtt ctttgcgcat
     8041 tttccacagt tcaaggattt catctgttga tgccataaag tttgaccaaa tgtcataatg
     8101 aactgggata acgactttag cacggagatt ttctgccata cgaagtaggt caacagaagt
     8161 catcttgtct tgaatcccaa ttgggttttc cccatagtta tttaacacaa cgtcaatatc
     8221 ataatcacgt ccatgtttag caaagtagtt cgaaaaatga gaatccgcac cgtgatagat
     8281 ggtaccacct ggtgtttcaa aaatatagtt aacagctttg cgagccatgt catcatctgt
     8341 gatagctaaa cctgctaaat caccatcttg agcatcagct ccttgtattg ggagagttac
     8401 cagacaggtg cggtcaaacg actctacagc ggtgatctta atatctttga attcaaagct
     8461 atcacctggc tttaagatca taatacgatc ttttggcaca ccccaatttt tccaaacttc
     8521 accacattca taaggtccta caaatttaac atgattaagt tttgggttat tgataatggc
     8581 tgccgcagta ttgatatcga tgtggtcact atgataatga gaaaccaaat aataatccaa
     8641 ctcattaatc ataaatggat caatcaccat tggttgggcc ctaagattgg gctgtaattt
     8701 acgagctcct gccatattag ccatttgatg tccacgaacc atatccttaa cttgcttggt
     8761 cgctttccca cgatttgacc ataagtccat gacaacatta gcaccgcctg gtgtcttaat
     8821 ccagatacca cagtttccta accaccacat cgcaaagttg tcagctggca cgacttcttg
     8881 ctcgatttct tcatttaacc atgttcccca ttctggaaac gtgttaagga tccatgattc
     8941 acgtgtaata tcttgtactt tagtcattag ggtttcctcc gaatttgttc ttcgtttcta
     9001 tactttaagt atagactttc ttgtcaatga taaaaagacc aagtgtcaca cagatctgtg
     9061 ttcaaaagtg gactgacttt ttcattagct cagtctaata ctatctcatt ttgcacactg
     9121 tatctcgcat caatactgta ttcaaagcta tattaaaaac ctcagttata tttttctatg
     9181 tcatagaaaa ctgaggtact ttgacgactt aacacttact catagcagct cgcgatgaat
     9241 gagtttttca attttagaaa ccaaggcata acgatctgat tcatctttta caacagcttc
     9301 aatagctttt gtgagctctg tagaaaagcg ggagtgctca ctcaagcgtc cttgctttga
     9361 aaagcgtatc aatttgatga tatcatcgtc acttaaaata gggttaacaa taagcatcgg
     9421 gattttagtc tttagcgtat ctgtagtagc aacaatcata tcgactgcta aaaggtcgta
     9481 aacactctga tactgttctg tcgtaaaaac agcttcaatc tgaccatttg ctagatacct
     9541 ttgacactgt ttgaacaata acttttgaat gcctatccca tcatctgaaa caataacgag
     9601 tttggtcttt tcttgctctg tattattgtg acgcagctct ccccctagat ggatagtcaa
     9661 gtaggcaata tcatcgtctg tcaagctaat cgtccaatct tgttccaaaa tagtggcaca
     9721 agattgagtc atagcaaaca attcttcgta tttttcttta atatggtctg ttaagggatt
     9781 aactaaaaaa atcccataag ctttacggta aacgagcgcc ttacagtggg tcgtcaactg
     9841 cttcagcaaa tcctgtttat gcgtgaaatg taactgataa cggctctcca actggtcaat
     9901 aaagtgacta atggttgctc gcatgtcatc ataatcttga ctttccacgt gatgatcttg
     9961 atcttttcga aaagacaaca tcaacatcgc taccatacta acttcgatat catccaaatg
    10021 aagcttaaaa ttatggtaaa gctctctagc taagtcctga gcaatgtgat attctttacg
    10081 tttccaaatc aaatgaaatt cttgtttaag agcagacttt gtttctgaat caagacgcat
    10141 attacgataa cttaaaagca tgaaaggtaa aatctgcaac atgaattggc tatcttgggt
    10201 attaattgtc tttcctaagc tagcttgaga taaaaagaga tactcatgaa aataagtcaa
    10261 aatatcttta gaaaaataga ccgataagcc ttgtatctct gagagtttac ggttgaatat
    10321 atcaataaag ctagtattgc caccttggta aacgtccact aacaatttgt acaggtattg
    10381 aataagagcc attggatgac aaccaaaata atatccccta gcctttgtcg catgaagctg
    10441 aatcttgtat tgtttgtctt ccagttcttc ccttaactca gtcaggtcat ttaaaatcgt
    10501 gttacgagac acatcattga tctgcatcag tttatcaatg gtcacgcgct cagtggaaac
    10561 cgcaatataa atcgaggata gtcgtctgcg ctcatcactt ttcatgacat aattataatc
    10621 agtcacttct tctaataata agcgacaagc agctttttga tccgccgtta aaagaatgcc
    10681 aagtctagga taagagataa tctgatctac tccctttggc aaagccttat taattttgtc
    10741 aagctggtaa taaactttac gacgtgactg atttaaagca tgagaaatgg ccataactgt
    10801 ctcaggtgtt tctagtttaa gtaaataaga cagcaagtca taactctttt tatctaaaat
    10861 catcatgttt acaaacctcc tctctgaaaa tccatccttg cttattttat catgatttca
    10921 gtgactgcgc agccaagttt tgaaaaactc accataataa gattcgctta atatgattcg
    10981 agctaatcac ctcaactgat ccaacaccaa caccttatat ctggtctcta agaactccac
    11041 aacatacgat tcatccagtt tgtgctcatt ggcgtttaaa cagttcatcg aaaaggacaa
    11101 gtcaaaagga cttgcccaaa caatgattaa caaaaccttg aactttttgg cttctagtga
    11161 gaacctgtgc taataagcca tatcgcgacc tcatttttga ccgtaataag catttgggcc
    11221 atgtttacgc aaataatgct tatccataat gtatcttggc gctggctcta cgcgtggatt
    11281 gatttgttct gtcaagcgat tcatcttagc cacttcttct aagacaaccg agtggtaaac
    11341 ggcttgctct ggggttttcc cccacgtaaa tgggccatga ttacgcacca cgattcctgg
    11401 aaccgccatc gggtcaagac ctcgcttgct aaactcttct aaaatcacgt tgccagtttc
    11461 ttgctcgtaa gccccatcaa cttctgcttt tgtgagagaa cgagcacacg gaactggtcc
    11521 atagaagtag tcagcatgcg tagtcccata aaacggaata tcacgacctg cctgagccca
    11581 accgacagct tctgtggagt gagtgtggac aataccgccc acttctggcc aagctttata
    11641 aagttccaca tgggttggca gatctgaaga agggttcaaa tcaccttcga ccacattccc
    11701 atcaaggtct gtaacaacca tattttctgg tgtcaagagg tcgtagtcca ccccagaagg
    11761 cttgatgaca atacgtccta attcacgaca aacttcagag acattgcccc acgtaaattt
    11821 gacaagaccg tgttgtggta gagacttgtt agccgcacag acacgttcac gcatttcttg
    11881 caagtttttc gccatattat gccaaccccg ctttctcaat aagtgggtaa aggaagtcct
    11941 gagcttcttt gatagcagct tttgtttcct ccaccgtgtc acaattttct gaccacattt
    12001 caatcaaaaa aggcccattg tagttggttt tcttgaggac cgcaaacagc tcttgccagt
    12061 ccacacaacc ttgaccaaat ggcacatcac gaaactgccc ttttgaggtt tccgtgacag
    12121 cataagtatc tttcaagtgg agggctgcaa tggatttgtg cccattgtaa aattcactcc
    12181 acaaatcatt atgccaagca gaaacattgc ctgcatcagg ataaacaaag agataaggcg
    12241 agtcaatctc tttttcaact gccaagtatt tttcaataga gttgataaaa gggtcatcca
    12301 taatctcaat agaaagcatc acttgggctt cttcggccca gtcacaagat tgtctcaagt
    12361 ttttgataaa acgagcacgg gtttcaggcg atttttcttc atagtaaacg tcataaccag
    12421 ctaattgaat ggtacgaacg cctaagtcct gtgccaattc aatacattgt ttcatcagtt
    12481 taagggactt ggcttcaata gcaggatcat ttgagcctag tgggtaacgt ctatggccag
    12541 aaaaacaaat ggtcgggatg cgaatgcctg tttcataaat ggctttcact aaatcaagcc
    12601 gttcttcctt agtccactct aagcgcgcta agcgagcatc tgattcatca acagacattt
    12661 ccacaaaatc aaaacctaag tcttttgcaa attgcaagcg ttcacgccag gtaaactgtt
    12721 ttggcgttgc tttttcataa ataccaatag gacgcgccat aaagttaccc ccagatacgt
    12781 ctgatttcat ctttgaaggc acgcgccgct gcagcaggat cttctgcttc agtaatgcct
    12841 cgccctgcga taaaggtaaa gacatcaacc ccttcaaaaa gtttcaaagt gtctacatca
    12901 agcccaccag ttactgacac gcggaagccc atgtcaatca atgttttcac cttgttaagg
    12961 tctttttcac cccaagtttc tccagcaaga agggcatcac gagattggtg gtaaatcgct
    13021 tgggaaatcc cagcgtcaag ccataattga gcttgctcat aagtccaatc accataaagt
    13081 tcgatttgaa tttccccacg gtcaccgcgc tcttctttca tagccttgag ggctgcttcc
    13141 atggttggaa tcgtcgcaca gcaaatgcag gtcatccagt cagcaccacg ttttgcattg
    13201 tttttagcga ccgtaccgcc agcgtcagca catttggtat ctgctacgat aattttctct
    13261 gggaagaggc tgcgtaatac ctcaactagt tcgcttccca cttgaagcaa acaaacggta
    13321 ccagcttcaa tcacatctac ttcgtgaccc acagcaacgg ctgctttaac agccccctgt
    13381 aagtcagaat ggtctaaagc aacttgtaaa tttggaatat gtgtcatctc tttttcctaa
    13441 aactcttagc caccgtgcaa actggttaag agttcttatc ctttccatca ttcgttagta
    13501 tcctgtgtaa ggaaagctta agcattcaag tccatacctt ctaagtaagg actatcttta
    13561 gattcttcta ccatttctag gacctcttta ggactttggc aggccaccaa gcgttcaatg
    13621 gcgttatcta gttcaaacaa ggccacaatt tgtgggatag ccactgttgt gtgaatagaa
    13681 gggtctgtag cggccaacgt caaaagaaca gaaacctctt taccatctga gaaggtaact
    13741 ggttttgtta gtgtaatcaa agcaaatgca ttgcgattaa cgcctaaacc tgcttctgca
    13801 tggggcatag ccatacctgg catcaacaca taataagggc cgtatttttc tgttgatgca
    13861 atgatggcgt cgtagtaagt tgacgtcact gctttgctat caatcaaagg ttgaacagct
    13921 aatcgaacag cctcttgcca agtatcagca cttagcccca atcggatgga gttgttgtca
    13981 ataaaggctt gttttaaatt cataaaaact atctctttct agtgtcttgc tttgcaacac
    14041 acaccatttt tactagtttt atcctactaa agagaaagag ctgaaaacct tcgtcacaac
    14101 tccttcgttt tgattcagaa ttagcttata gcacttcttg tagtttggtt ttaatttcat
    14161 tatcatccat caggttatca agaccaacca agtggccttt ggtacgacca tcaagctcat
    14221 gaatcaagtg gtttgatgcc actacaatat cataaccaga agctagacct ttagcttcac
    14281 caactgagca agaagctgac tgaatatctg tcacgccaag ttgacgaagc gcattttcaa
    14341 ctttcatttt gatcaccata gatgacccca taccattgcc acatgctgtt aagactttaa
    14401 ccattgtttt ttcctccgaa aatgttttat tattattttg ttaaaatagc agagctattt
    14461 cttaagatgc agtgttattg agcttcccca cggtagtaag cttctttgtc cttagctttt
    14521 gcgaactgca attgtggaat ggcaagtaag aagatgcaaa caagcacata ccctgcaata
    14581 ccaaggaatt tgaagaagta cccaaatggt aaccatggaa gaaccaagtc gatattaccg
    14641 tgataaccac ctgtaagtcc taaaagacct acagcaacag caccaagtgc cacctgaata
    14701 atacctgaga taaatgatag agctaccgct gctttccagc caccacgttt atctgcataa
    14761 accgcaattg ccgcattatc aaagaatacg ggaacgaatc ctgtgataat taagattgga
    14821 tttttaaaga taacaagaag agcaatggtg attaattgac caatcaaacc aaaggcaaat
    14881 cctgaaagca ccgcatttga agaaccaaag ccataggaag ctgcaacgtc aaccgctggg
    14941 aatgaccctg gaagcaattt acttgagatc ccttgaaagg cattggtcaa ttcagaaaca
    15001 aacatacgaa caccctgcat caagataaag agataaactg agaaggtgag agaggtttgc
    15061 aaaatataca tgaagaaagc ttgcttagct gggttgaatg ctccaggtcc gattaagtcc
    15121 acatcagaca taatgtctgg accaaggaca gccaagatag ctccgaaaaa gaccaacatc
    15181 aaagttgctg acgcaacaac agtatcatgg aaaatgttta agaaggttgg taattttagg
    15241 ttatcaaggt tttcttcttt tttaccaaag aacggtgcaa ccttatccac aaaccaaatc
    15301 gcaaattgtt gttggtgacc aatcgcaaac ccaccgccac ctgtaaggcg ttgagttgct
    15361 tcaacggtca tgtttgagct gatagcccag taaagaccac agatgatacc aactgcccat
    15421 gccccaaaag cattttggaa ttgagggatt aaaagaagga caaagaccga aatcgtcgct
    15481 gcctgttgga ccatgatgtg acctgtgatg aaaagggtac gaactttagt gaccttgcga
    15541 agtgcaacca aaagaatgtt gaccccaaat ccaatcaaga gagctgtcgt agcaacactg
    15601 atgaagccca tctcttctaa cttagtatta gcagcagcca aaccaaaata aggatcaata
    15661 acggcggctt tcaattcaaa tttcttggca agagcaacca agatcggacg aaaagtcgtt
    15721 acaagaccgc ctgctccaac gttcaaaatc aagtaaccaa cggtagcctt tacaaaacca
    15781 gcaaagacct cataaatggg ttttttaagg agtaaatacc cgattaaaac caaaagacct
    15841 acaaagaacg ctgggttttg caaaatattt tgcgagaacc agtttagggg agctaggagc
    15901 atttccataa gaactacctc ttttcaaagt taataaagat aaagtgttta tatgttcatt
    15961 ataggtaatg aaagcgtttt aaaaaagacc aactatcaca caaagaagtg ttcaatatta
    16021 gacttaagtt aacttatttt aaaatttaac tacacttagt aaaaaggaga aaatcaccct
    16081 aaacaacgat taaaaaccac aagaaccttc gttcttgtgg tttttctaga tgagttgctt
    16141 tttgaataga tcagctcttt ggcattaact gtgacgtgac gcaaataacc actgaagggc
    16201 tgtgtcacct caaactattt gataacaata ttaacgagtt tgtttggtac tgcgatgact
    16261 ttgatgatgt ctttgcctgc gatttcggct tggacttttt cgttagcaag agcaacttct
    16321 tgcaattctt cgcgtgaaag gtctttagca actacaagtt tagctttgac cttacctttg
    16381 atttggacaa cgatttccac atcgttttca accaatttgc tttcatcata acttggccat
    16441 ggcacgtaag agattgactc acctgaggct gtcaaggctt gccagagttc ctcacccaag
    16501 tgtggcgcaa atggtgcaat caattgcaca aagcctttgg catagtcgct aaagagtttg
    16561 tcttccttgt tagctgcatt gacaaagacc atgagttgag cgatagctgt gttgaatttc
    16621 atttggtcaa cttgctcagt aacggcctta acggtttcgt tgtaaacctt gtcaagggct
    16681 ccgctatttt cctcagtgat ctctttagtt gtgatcaaac ggtaaacacg gtccaagaac
    16741 ttacgtgagc cttcaagacc ttcttcagac caagcgatgg aagcgtcaag tggtcccatg
    16801 aacatctcgt aaacacgaag agtgtcagca ccgtactgct caacaacatc atctgggttg
    16861 acaacgttct taagtgattt agacatctta gctggtgctt gttcaagctc ttccccagtt
    16921 tccacatgga agaagctacc atcacgtttt tcaaccttgt cagttgccac aagagcacca
    16981 cggctatcgc ggtagcttgt ccccaaaatc atcccttggt taaagagttt ttggaatggc
    17041 tctttggtag gtacaacacc gagatcgtaa agaaccttgt gccagaagcg agcgtagaga
    17101 aggtgaagca cggcatgctc tgcaccaccg acatagatat caactggcaa ccattgtttg
    17161 aggagttcct catcagccag tttttcggtg ttgtgtggat cgatgtaacg aagataatac
    17221 cagcttgaac ctgcccattg tggcatagtg ttagtctcac gacgaccttt cacaccatct
    17281 tcacgagtca cttccaacca gtctgtcacg ttagcaagtg ggctctcacc agtacctgaa
    17341 ggacggatat ccttcgttac tggaagaacc agtggcaact cactctcagg aaccgctgtt
    17401 gaggtcccat cttcccagtg gatgattggg attggctcac cccaataacg ttgacggcta
    17461 aagagccagt cacgaagacg gtaagtgact ttctcattac cgacgccttc tgcttcaagc
    17521 cattcgacca ttttggcaat agctgacgct ttgtcaagac catctaggaa gcctgagttg
    17581 atgtgaagac cgtcttctgt aaaggcagcc tcttcaacat taccaccttc aagaacaggg
    17641 atgatgtcta ggttaaattg ttttgcgaat tcccagtcac gttcatcgtg ggctggaaca
    17701 gccatgatag caccagtacc gtagctagca aggacatagt ccgcaatcca aactggcatt
    17761 tctttgccgt tgaccgggtt aatagcgtag ctacctgtcc agacaccagt tttttctttg
    17821 gcaaggtctg tacgagcaag gtctgatttg agacttgctt gacgtttgta gtcagccacg
    17881 gcttctgcct ggtcagctgt tgtaatagca tcaaccaagg catgctcagg agcaagaacc
    17941 gcgtaagttg caccaaaaag ggtgtcagga cgtgttgtaa agacagtgaa gtccttatct
    18001 gtatccttaa ccttgaaggt tacattagca ccagttgatt taccaatcca gttgcgttgc
    18061 atgtccttga ttgattctgg ccagtcaact tcttccaagt cttctagcaa gcgctctgca
    18121 taggcagtga ttttgagcat ccattggcgc atgggttttc tgacaactgg gtagccgcca
    18181 cgttctgatg tcccatctgg aagaacctct tcgttggcga tagctgttcc caattcttca
    18241 acccagttaa cgggaacttc agcttcgtag gctaggcctt tttcataaag cttggtaaag
    18301 atccactgcg tccacttgta gtagttaggg tcagttgtgt taacttcacg atcccagtcg
    18361 tatgagaaac caagagcatt aatctggcgt ttgaagttag cgatgttttc agctgtaaac
    18421 tcggctgggt cattaccagt atccatagcg tactgctcag caggaagtcc aaaggcatcc
    18481 caacccattg ggtggaggac attgtgacct tgggcacgtt tgaaacggct gagaatatca
    18541 gttgctgtat agccttctgg gtgaccaacg tggagaccag ctcctgatgg gtatgggaac
    18601 atatctaggg cataaaactt aggttttgat gcgtctgttc ctgttttaaa ggtgtgatta
    18661 tctgcccaaa aggcctgcca tttgggttca atagcggtgt gatcgtaaaa tgtcatgtga
    18721 aatctcccat gtaaaaaatc tagtcttctt attatagcat gagatgaagc ttttaggaat
    18781 ggtcaagaca tgactagcac cttggctttt gagacttcag acgattagct atttttaaaa
    18841 cctgctttag catcaaccat cctcacaaaa agctaataaa aaaagccaga cttgctctgt
    18901 aaacagagcc aatctggaca aatagactta aaaagcaagt gcttgctcaa aatcggccaa
    18961 taagtcttcg atattttcaa caccacaaga caagcgcagc aaaccatcaa caaccccttg
    19021 ttccaaccgc gcttgtgatg ataaacaagc atgagacatc gttgctggat gtgacaaaat
    19081 ggactctact ccacctagac taacagctaa aatcggcaac tgaatacgat gggtaaaggt
    19141 catcagctcc tccttagaag ccaactcaaa ggataaaaca gcaccaccat tcttagcttg
    19201 ttgcaggtgg gtatcgtgat taggatgact gtctaagcca gggtaatgta ccttaagaac
    19261 tttggggtgc ttcgctaaat agttagcaag ctgctgagca ttttttaccg cttgttccat
    19321 acggatcccc atagttttca tgccacgaag aatcagccaa gcgtcttcga ccccaacaat
    19381 tgctccaaag tttttttgaa aaagcttgag ctggttgtag atagcctcat tattggtagc
    19441 agctaaacca gctaccacat cactatgacc attgataaat tttgtcacac tttctaccac
    19501 aatatccaca cccatagcta agggttcttg atacaaactg gtcataaagg tattatcagc
    19561 cacagttagc acaccataag ctttggcgag ttgcgcaagc tctgaaatat cacaaatttt
    19621 caagagcgga tttgacggtg tctctagata aatcatacgc gtattaggcc tgatacttgc
    19681 ttttactgtt gctaaatcag ccatatctac aaagcttgtt tcaatctgat aattgggtaa
    19741 aatagctgtc gcaaattgac aagtccctcc atagacctcc aatgggaaaa tcacatgatc
    19801 tccagctttt aagagcatca aaacagttga aatagcagcc ataccagatg cataagccac
    19861 cgcataagtc gctttttcta aacaggctaa cccgtcttct aaagcttcag tcgttgggtt
    19921 tgaaaagcgt gtgtacaaat gtttttgact aggacaatac agttctgaat tgtggaacgt
    19981 cgaagtctga tagatgggaa ctgaagctgc tcctgtaaac tcgtcaataa ccgtatagcc
    20041 atgtaataag gtcgtatttt ctctcatttc gtgcacctcc gcaatagcgt taactccaat
    20101 gaactctctt ttagacaatg gtcccacccc aatggttgca caagccaaaa agctcttcaa
    20161 aaaagcgtga tctgctaacc tataagaaaa gtagtggaca ccttttaaat ggataatgaa
    20221 tttttccaac aatatcattt ttagatcgat tacgtttatt atacaacctt tagctaagtt
    20281 tgcaagccct tttatcatct ttgaccaagc tgccaacacc tctccagcaa gttaaagtat
    20341 agctaccttt ggccccatcg tctcaaaagc tcaaattatc acactgacag tcctaccttg
    20401 catcgtcacc tactcaatat ggcaatactg ccatcaaaat ttcattaaaa gttcataatt
    20461 tatttcattt aatgaaaaac agaacttgtt ttcaccatgc gtcttatagt acactgctaa
    20521 agtactattt atttttagga gagaaatata gtgaaaaaca aattattttt agttgccctt
    20581 gcgaccgtaa ctgtcctagg gccctcttta gcaacccctc atcatcagac cgtgcatgcg
    20641 gctgagcagg caaaagaacc ccaaaccagc tcaatgacgt gtactaacga tggatctgta
    20701 tgcgctggct ggcatagttt atcagaagga gacagtataa cttcaaattc atcttcaaac
    20761 accaagactt ccctgttaaa ctggttatgg agcttaataa caaatttgtg gaattctgca
    20821 aaaaacttgt tcatctaaga tctctataat catatccatg gaatttaaat aaccatctcg
    20881 tggcggtttt ctaatcctag gtcgccacac ttcttattca gataaataat aataaaaaca
    20941 ttaaaataac acttgattta ttaacaaaaa gttagtataa tataatcaca atacaaatta
    21001 aaggagttat aaatgaaaaa gaaattatct ttatttatga tcgcaactgc tgctgttgca
    21061 ggccttagcc ttgcaacact aggaagccac caaactgttt cagcaaatga tgctagctat
    21121 agtaacggtt tctacggaca cactggtcct gacagctggt tactttacac agtatggtaa
    21181 acctaatacc gtaacataga agctatcttc ttcgtaagac tacggttatg aaaagcctag
    21241 agctgcgtaa gcagttctag gctttttgat tggctaaaac gtttttacta acgatccctc
    21301 aagattaaaa agaaaaggca taccgcctat aaaaggacaa gcccaaaaca caagtcgtaa
    21361 cagttgctag ctaaccacca ccaaaaaagc tgtaacacct caaatataat tataaccaga
    21421 ttaacattat taataattaa tgttatttat ataagataaa gaaatgcttt agtatcggct
    21481 ttctttgcct ttttcggtca aacacataac aaaaagatta aaatgtgata ttgaatcatt
    21541 aacataatat gagtataata taggtatact ataatttaag gagttacaaa tgaaaaagaa
    21601 attatcttta tttatgatcg caactgctgc tgtcgtaagc cttagccttg caacaccaga
    21661 aaaccaaact gtttcagcaa atgacacgca agttacttgt gtaggatgtc cagattgtgg
    21721 tccaaaatgg tcttgttgtc cattgacctg tatgcgcgat ttgttaagag gttggggcaa
    21781 gtcagcagca tattattgca cttggggata ttactactct gagtaaaaat tacttgagag
    21841 tctgagacag tagtctcttg attatgctaa aaaaacaacc tttcacatta gcagttaaag
    21901 gctgttttta attcattttt attatgacat gctttgtact gttgtagttg cctggttatt
    21961 ggggaagaat tgctgtagcc aatgagagat caaatcccat ataaccttga ccggagtcaa
    22021 gacgggatgc tgagatttcc agccttcatt ataaccatct ctgtacccat ctgtataccc
    22081 gtctttataa tgctgtttat tagtagtctc tctgatatca ctaggtggtt ctggaatggt
    22141 tggattagtt ggggtcaaat cagtccaact agcttctttt ccagctgttt tccctgcttc
    22201 ttctccagcg cttttccctt tataatatgc tgcattagct gggtcattat tctccccatc
    22261 tgccataaca ggtcttgcta taagcaacat agcagcaaat agagccacca agaggtagac
    22321 gaagcgtaga ggctggtaag attgttgttt catgataata gttatctctc ccttttatgt
    22381 tatgctttgc ttgttaaaaa gcagcttgtt agtattatag attagaagca gcttagcttc
    22441 aaggtcacga tgtgagaaaa gaataatttt ttgattagta tatcaaaaaa gcttgtctat
    22501 gaaggtatct catagacaag ctttcttctg tatttgtgag aggattaggc acaaggcctc
    22561 aagagataag cagcatcaag gtgcttgacg aacctcttga accagtccta cttataagta
    22621 atcgaaccat atgggctcaa ggttgatcct gagatgttga cattgatttc tttagacagt
    22681 ttcacatctc tttcgtcgat cacttttcgc caccattccc aagctaagcc ggtgcactct
    22741 ctagccatga tacggatatt tcgtgaatta gctcctagtg ggataactgt gctaaatggt
    22801 gatgtcttac tataccagtt gttgtcccaa cgtcgttttg taatcacttc ttttcctttg
    22861 tcatcataat tgatttcatc ccaaaggatt tcatattgag caacataggc accttgatga
    22921 gacaggttaa tttttccact cgtgtactcg gtagatgttg tttcaacgta ttcacttctg
    22981 ttattgacac ccgcaatttt attattttta aggaaaacac tggtgtatga aataggataa
    23041 gctgggtttt ttctactgaa ggtagcatta tctttgataa cgtttctaat aacatcaaag
    23101 tcttttgtga ctaccttatt gtgctctgca gcatctcctc ctaaaacgac agctgtaaat
    23161 gaactatttt ctaaaatatc agagtatttt ccattagttt taacatctgt tccttttaga
    23221 gctgcactaa aggccgcttc aacatcatta cttttagaac ttgtttctag tttgacaaaa
    23281 acagttcgac cataagctac gttactcaca aagagtggcg gggcttcatt gctgacacct
    23341 tttcgttgca actctttaaa ggtcactgat ttatcaaaca catccgcagg attattagga
    23401 aggtttgctg atacggtgta aaaaatttgc ttgtatgctg caatcatcac cttcttttca
    23461 ccttttgaaa tcgacttgaa atcaatgcct aaagtaccat ctaagatttt actattaaca
    23521 tttagagctg cttcaatctg tgatttagaa tataccattg attcagtata ttgtgttctg
    23581 gcaggaagcg tattaccacc agaataatta tcatgccatt ggttaacaag attatcaata
    23641 gctgttgaaa cattggcata ggtagggtca ttgacctcaa ccgttgcttt gtctcccata
    23701 cctggtaaat caatatggat tttttgtggg tttcgcttgg tgactaccgc gtctggtttg
    23761 ttttcggtaa aacctttatt agccagctga agggctgctg gataggtcct atcagtgaca
    23821 gagtcaatga tggaaatatc gactggtgta gtgttgatat tttttttctt tctttcaatg
    23881 acaataaatt tatcagcttt cttaacgcct tctttaggaa caaaattttc aatggtttca
    23941 ccatttttag caagtacttc aagctcatta taatttagtg aataaatctt gtcattgatt
    24001 tcttcagtgt gatcttcttc gctctttttt ttgtcttctg actttttttc ttctttttct
    24061 gcagattcta gtggcatttc tttgggagca agcttaatca tatcgttaga gttaagcata
    24121 tcatccattt tctgacctgc tttttcggta gttagctcac tactttctgg ctttggttgc
    24181 tcatttgtcg ttgtggtttc tgtactagca gtgttttgtt tgttcgattc agcattagca
    24241 gtaacaagat taccaatgat aagagctgcc gttagtagcc cagcgacgcg actgtatttt
    24301 ttaaatgttt ttttgttaga catgtccttc ataccttttt atcattctaa aatgtttcta
    24361 ttgttctttc gaccatatca agcatagtat cttgatagtg agcaaattct cctaatttgt
    24421 aatgtaccat agcctctcta atatgcgcat cttttaacgc tttggttata ttagctagta
    24481 tgctttgacg gtttaaataa aatggcaagc tttcgtaata atttaataac atgttttcag
    24541 cgtttagggt ttgaaccgct tggtaaatgt ctaatgcttg accgctattt gacatttcta
    24601 ataatgcttc ttcgatgtta gctttcaatt gggtatagcc ttttatgaaa gcagggtaaa
    24661 ccccatactc taaaataact tctggatcag aaatatctcc catataggag tctcttctag
    24721 tgatacgata ctctttgtca ctacctatca agtatttttt taaatcatta acagttactt
    24781 ctttttgaaa cattttttga taatgttcta aactctttgg caccttatac atattgttac
    24841 ttcctatctt gcattctatc ttgcattttc ttaattttga agcgaccagt tactttttta
    24901 tggtcaccat ttggagcaat gatggtagga tcaataattt caaaatagcc tttggcatca
    24961 atctctgcta agacagcttt agattttaga tctttaacac taatcgatgg gaaaattaac
    25021 tcactttctg aattttcaat gtgttttcct gaccacgttt ggcaaccagg aatgttgagc
    25081 gaatttgctc caggggtatc tccgctagct gttcggatat agcctagctt atcaatgttt
    25141 tttgttagtt cagaatcgta ctcatattga taaactccat cgctatattt tccgctatcg
    25201 acacctttta tattttcaat ttggtcagct gattttaact taccttgatc agtaaagagc
    25261 aatgccatat cttcagcaaa acttgtcaca aaaagaccgt tttcttgtcg accaatcgtt
    25321 gactttcttt tagcatcatc aaaagctaac atcaaatctt ttttacttgt aacgtgagaa
    25381 gctccgcttt ctttgtagac ttgttttagt ttttcaacaa actctttttt gtagtggtat
    25441 ttgcggctaa cgtacatccg gtaatactca gcacgtgacg caacagcaga catctgaaca
    25501 gctttatcag catacttgtc ggcagtttca ataaactcat ctgcttgttt atctgttacc
    25561 catttatctt taatttcttt aaactttttt tcaaaaagat cgcgttccat attgccaaaa
    25621 gtgacctctg acaaggctaa aatatccttg ttttgtccct gatggacctc tgttacctca
    25681 atatcaatac gatcaacatc taatttttta tcaatctcaa tgtgatgcgg ttttttagtg
    25741 taggtgtcgc ctaattcaat tttttgaaag aggaccaatt tattcccgtt atagtattta
    25801 acatcagcct ttgcaattct atttttcttg tagtaattct ccttgctaga tgtatctcca
    25861 ttcacaatta aaattctccc aatacgcatc ggggaaacaa acttgtagga aagtacctca
    25921 ccaacgccac caccaggtga attttcctcc cagacagtac tcattgagcc gtctaatgtg
    25981 tgcatgacat gattgtcttc tttagaggaa gttgcgttag cttccataac tttggtcgtt
    26041 tcatatttga catcgctttt tttattttct ttgccactaa cagcgtacgt tgtgctatta
    26101 gctaataaac tgctacccat tgctccagcc aaagcaatag caacacttgt ctttgcgaca
    26161 atatgagcta atgttacttt tttgtttctc atgtaaacca ccttatatta tttttaagta
    26221 aactatacat taacaactag tttttgtcaa tgcgatttat taacatcgtt ttaggctttt
    26281 ctatcaaaaa aatgaacaca aaaaaacaag tagccaactg ttacaagttt caaggggata
    26341 tttccatctt attatcacag aaccttcaca tctgttctat ttaaaccaga ttaaaagtac
    26401 tagttaaata tttgatagta gcaccgttat cagtaataga tctgttaatt atttctgatt
    26461 acaatgatgt taacaatagt catccattgg tttgtaaaca cgcttcactg ccaactaaaa
    26521 aaggtctgat ggtaatactt ataccatcag acctttgaga gcctatagtt ctgcaatttg
    26581 ataaagttca acttctgcta ctgtttcaga gccaaacatg ttgagcatca gcttcacttt
    26641 gttgttttcg atttctacca cacgaccttc ttgtcccata aaggcaccgt ctatgatctg
    26701 aaccacatca ccttctttga tattggtatc aaagacatca atggtttgac ccatagacaa
    26761 caagatagca cggatttctt cttcaagaag tggtgttggt tttgatcggt tcccgtgtga
    26821 tcccacaaaa cctgtaacgt ttggtgtgtt acgcacaaca aaccaagctt catctgtcat
    26881 gaccatttca accaagacat accctggaaa gcgattttct tcgatttctt tggtttgccc
    26941 atttttttca acgttaaccg tttgtgtcgg aatttcgacg cgcaaaatat tgtccaacat
    27001 gttataggtt tgggcacgtt gcaagaggtt ttcttttact ttgttttcgt agcctgagta
    27061 agtttggagg acgaaccagc ccttatcaaa tgaatctagc atgatgtttc ctttcattat
    27121 aaaaaagccc tttttttggc ttttgcgctg ttttctatca ccttcattat aataaaagac
    27181 agggctggtg tcaacgtatc tgtggagcta cttgacacct ttattaaaaa gtccaaagtc
    27241 ggttcgtgac gactctggac ggtattgtac agttttagtt ttcaggaact gtgatggttc
    27301 cttcgataat cgctttttta gcagtctcaa tagctttttt agtgtcttgt gtcagagcat
    27361 ccgttgtaag gctaacacca ccttctttta aaccaaaagt ggttatttga ccacctggga
    27421 attggccttc tgaggttttt acggctactt ttactaaagc ttttccgact tccttgatac
    27481 ttgaggtcaa aacaaaatta gctgacttgc catcttttgt ggtgtatttt ccatcttcac
    27541 tttggtcacg gtcaacacca ataacccaaa ccttatcttc ttctttacgt ttttcgttga
    27601 tagacttagc ttcgctaaag acacccgccc ctgtgcctcc tgctgcatga taaataacat
    27661 ctgcgccttc agcatattga gcagctgcaa tcgtcttgcc tttggcagca tctgcaaaag
    27721 agcctgcata agcaactctt actttgatgg tatcatctac tgatttcaca ccagcttcaa
    27781 aacctttttc aaagcgcttg acaacatctc cttccatacc acctacaaaa ccaacttgct
    27841 tggtttttgt cgttttagct gctgcaacac cggctaggta tgccgcttca tggtctgaaa
    27901 aggtgatact tgcaacattt ttttgacctt taatcacatc atccacaatt gcaaaatggt
    27961 tgtcaggatt gtttgcggct actttttcta cagcgtcatg taatggaaat ccaatcccaa
    28021 aaacaagatt ataaccattg gttactgctg actcaaggtt tgtggtaaac tctgattcat
    28081 tggctgattg gaaataatca tagccttttc ctttttcaag tttattttct tttccccaag
    28141 cttgtaagcc ttcccaggct gactggttaa atgacttgtc atcaataccc gtctgattag
    28201 tgatcatagc gatttttaat gaattgtcag ttgtttgttt agaggagtta ttggtgcagc
    28261 ctcctaatgt gaatagggca gtcgaaacaa gaccaagtga cattactttt ttgttcatgt
    28321 gaaaatgaac ctcctaaatg tttaatccta aagtgatgtg tcattgttaa cgttctactt
    28381 atgctctaga acgaaaagtt tagtatatca aaaaaatcag ttttatccta ctatttttgc
    28441 tgaaaaatct ctttttcacc aaaatgaccg aacgagaaag tgagatcaac tggtaaaacc
    28501 cgttatctcc tcaaaaatca cctcttaagg agataagaca acgacaaaac tcaagctact
    28561 agtaatagca tgactaacta cacaatccaa caaacggtaa aaagagctgg aaacgctatc
    28621 caactctttg tcatactaga caccttagat agatgcccag accgactcaa gaatattggt
    28681 ctgttcacgt cctggaccaa ctgagaaggt tgaaatgcga acgccaacca attctccaac
    28741 acgacggaca tagttgcggg cattttcagg cagctcatca agactacgaa cacccgtgat
    28801 gtcctcttgc cagcctggta attcttcata gattggtttg caacgtttga gttgttcaag
    28861 acttgctggg tagtgatcaa tacgttcacc atctaaatcg taagccacac aaatcttaac
    28921 cgtatcaagt cctgaaagaa catcaatgga attgagggaa aggttagtaa tccctgacac
    28981 acgacgacta tggcgcataa caactgagtc aaaccaaccg acacgacgtg gacggccggt
    29041 cgttgtcccg tactcatgac ccacttcacg aatgcgctca cccacttcat caaagagttc
    29101 tgtagggaat ggcccatcac cgacacggct tgtgtaggct ttacagacac caactacttt
    29161 gttgatttta cttgggccaa ccccagaacc aatagtgaca ccaccagcaa ctgggttaga
    29221 tgaggttaca aatgggtaag ttccttggtc aatatcaagc ataacccctt gtgcaccctc
    29281 aaaaagaaca cgtttcccag catcaagtgc atcatttaaa attacagaag tatctgtcac
    29341 atattgttta atttcttgac cataagcata gtattcttca aaaatagcat caaaatcaag
    29401 aggtgtactg tcatacattt tttcaaataa acggtttttc tcagctaaat taatgcgtag
    29461 gcgctctgca aagatatcct tatccagtaa atctgcgata cggataccaa ctcgagcagc
    29521 tttatccata taagctggac caatcccttt aatagttgtt ccgattttat tgtcaccttt
    29581 tgcatcttct tgcagttgat ccagctggat atgataaggt aaaatgacat gagctcggtc
    29641 tgaaatgcga aggttatctg tcgtcactcc ttcatcatgc aggtacgcca actctttgac
    29701 caaagatttg gggttgacca caacaccatt accaataacg gaaatttttt gcgggaagaa
    29761 aattcctgat gggatcaagt gaagtttaaa cttcttacca tcaatcacga tcgtatgacc
    29821 agcattatca ccaccttgat agcgagcaat tacttctgca tccgctgaca agaaatcagt
    29881 gattttacct ttaccttcgt caccccactg ggtaccaaca acaactacag atgtcatatc
    29941 attgtcctaa ctttctgaaa cacttattct caataaaaat cataggcaag ctaaccactg
    30001 cttaaaaacc aaaaagaagg tcagatctgt aagatcagca atgtagctct tgatatttaa
    30061 agaaaacagg caggaatctc acctgctagc atatatctta caacttatta tagcaaaaaa
    30121 tgattttttc tgctagattt cttagcatta ttaaggaaaa agagaaactc tttttttaac
    30181 catgataaac aatcctaaaa aaacgaacat tataagagaa aagcttttaa cctcgtttct
    30241 cctaaacttt agcagggtta aacgattctc ttaaaacatt agctcttttc tttcagaaat
    30301 agcggtttta aggtaaaata atagtaagtt acctctatga gatagccagt aaaaacatat
    30361 cgaggaaata tcacatgatt aaaaagtatg accgcacgag cacaaaaaag aaatcactaa
    30421 actggatttg gctaatcatc gcttttttta tgatttcaag ctttataggt ggctcttctt
    30481 ttactgagtc acttctagac attttgcctg ccatagccat tggtggtaca ggttatgcta
    30541 tttttcgtgt tagatctcat caaaagcggc tagtaaaggc caagattgcc aaacaattag
    30601 aagatctaaa agcaaaaatt cagctagctg atcgtaaagt tcggctgtta gacacttatc
    30661 tggcagacca tgatgacttc caatataacg tactagctca gcaattatta ccacaactta
    30721 gtgacatcaa agcaaaagct attacgttaa aagaccagtt agatccccaa atttatcgtc
    30781 gtatcaccaa aaaagccaat gacgttgaaa gtgatattac ccttcaactc gaaacactgc
    30841 agattgccac aactcttaac cctcagcctt tgaaaacccc atctcctaat cttattaaca
    30901 aagcacctga gctcaaaccg tattatgata atattcagac agatcatcaa gctatccttg
    30961 ctaaaatcca aggagccgac aatcaagaag aattgcttgc gctgcatgat gctaacatga
    31021 ggaggtttga ggatatctta acaggctact taaaaatcaa agaagagcct aaaaattact
    31081 ataacgctgc tgctcgttta gaacaagcca aacaagctat ccaacaattt gatgaagatc
    31141 ttgacgaaac ccttcgtcga ctcaatgaaa gtgatcttaa agattttgat attagccttc
    31201 gaatcatgca aggtgctact caaagacgaa caacgcatca ccaaaaagac taaaggagtc
    31261 cctttatggc agattttaat tttgatattg accaaattgc tgacaatgct gttgtcaaaa
    31321 ccgataaaac caccgatatt atctcagact taccaactga cacaaatggc caaatctctt
    31381 tttttgagaa actaagtgct gaccagcaga cagctattac tgccaaagcc ccggcattgg
    31441 tagatacctt tttggcagac caaaatgcgc tacttgattt tggtcagtct gctgttgaag
    31501 gggtcaatgc aacggttaat catatcttgg cagagcaaaa aaaattacag attcctcagg
    31561 ttgatgatct tttaaaatca accaatcgtg aactcaatgg ttttatcgct aaatacaagg
    31621 atgccacacc tgtggacctt gataaaaagc caaactttct tcaaaaactc tttaaacaaa
    31681 gccgtgatac cctgcaagag ttttattttg attctcaaaa cattgagcaa aaaatggata
    31741 gtatggctgc agcggttgtc aagcaagaag ataccttagc tcgcaatatt gtctcagcag
    31801 aattgttaat tgaagataat actaagtcca ttgaacattt ggtaggcgtc attgctttta
    31861 tcgaagctag tcaaaaagaa gctagtcagc gcgctgcggc tttacaaaaa gacctaaaaa
    31921 ccaaagacag tgctacacca gactatcaaa taaaagctga tcttttggca agaactaccg
    31981 aagtcatcaa caccctcgaa caacaacaca ccgaatactt gagtcgcctc tatgttgctt
    32041 gggcgacaac tccgcaaatg cgtaatctag tcaaggtctc ttctgacatg cgtcaaaaac
    32101 taggcatgtt acgccgcaat accattccaa cgatgaaact atctattgct cagttaggca
    32161 tgatgcaaca atcggttaaa tctggaatga ctgctgatgc cattattaat gctaataatg
    32221 ctgcccttca gatgctagca gaaacaagta aagaagccat cccagcattg gaacaatcag
    32281 cacaaagccc tactttgtct atgaaatctg tgacaagtct ggcagaaagt ttagtagcac
    32341 aaaataatgg catcattgcg gcgattgatc atggacgcaa agaacgagca caactagaaa
    32401 gtgctattat caggtctgcc gaaaccatta acgattctgt caaactacgt gaccaaaata
    32461 ttgtgcaagc acttcttagt gaaggcaagg aaactcaaaa aacaattgat aaaacaactc
    32521 acgcataagc taaacatcac ctgtcacgct acttaaaaag caagccatca gttaggttaa
    32581 tccctttgat ggcttgcttt tttactactt aacgaataat cgccttgttt gatataacac
    32641 ttatctttta tgatgttatt ttcataatac gaacaaggct agcacgctcg gcctcttcta
    32701 atttcaactc aatgtagtga atcgtctctt ccagttgcgg gataatagca tactctaagc
    32761 cattgacgcg acgtctagtc ttttcaatgt catcagccat tagttgacaa gttttttcaa
    32821 cttcagctaa ccgtaagagc ttttctaata gttcttttgt cttttggata gtattgtcca
    32881 tctcactatt tgagctgagg taactataag caaactcacc ttgttcttgc tctcttgccg
    32941 tattagactg gacatggaat ttaggcacat tgacactcat gatattttca atatcaatcc
    33001 ataaggtcac ttcatggact ggaacagcaa agagttcttc taccattaga ctattttcag
    33061 atgcttttgc caagacaaac tccttcatat tggcagcaag ttctttttcg atagtttgac
    33121 gaagctcatt gttttcacga attaaatcaa cgaagcggcg catcaattca tctcgcttgt
    33181 cctttaataa tttatgacct cttgtagctg tcttcaatcg atttttcaag ttactaagtt
    33241 ccatgcgtgt tggtttaaca tttaatcgtg ccattgatat aactcctttc gtcaagggct
    33301 tgaaattgca tggtcaactc aatcatttgc aacaaaaacc tttgtcatcg tggtatctgc
    33361 tttaggcaaa taacgatcta acatatcatc tttaatgcgt tttaattcgg tacgtggcaa
    33421 aatcgacaac aattcccaac ccaagtcaag actttcttca atgctacggt tggtgtaaaa
    33481 tccttggtta atgtattctt cttcaaaccg gttggtaaag gccacatata gtttatccgt
    33541 ttcagaaagg gctgattcac cgagcacaac agccaattcc ttagcttgtt ttccttgggc
    33601 ataggctgca aagagctgat tcattgtggc agcatggtct tgtcgagtct taccttcacc
    33661 tgagccttta tcttttaggc gagatagcga tggcaagaca ttaataggcg gtctaaaacc
    33721 atttttatat agttcttgcg acaaaataat ctgaccttcg gtaatataac ctgtcaaatc
    33781 ggggataggg tgggtaatgt catcttctgg catggttaaa ataggaattt gagtcactga
    33841 cccttttttc ccgatcaagc gaccagcccg ttcatataag gtggagagat tagtatagag
    33901 atagccagga taacctcgtc tgcctggaac ttctctgcga gcggctgata cttctcgtaa
    33961 ggcttcacaa taatttgtca tatctgtcat gatgactaaa acatgcatac ctttttcata
    34021 agccaagtat tcggctgttg tcagagctat acgtggtgtt gcaatgcgtt caatagcagg
    34081 gtcattagct aaattgataa ataaaacaga tcgatcaatg gcacccgttt cacgcaagtc
    34141 attcataaag aattctgcct cttcaaaagt aatacccata gctgcaaaca caaccgcaaa
    34201 gttatcatca gagtttagaa cagttgcctg acgagcaatc tgtgcggcca attcattatg
    34261 aggtaaccct gagcccgaaa aaactggtaa cttttgacca cgaacgaggg tattgaggtg
    34321 atcaatagct gagatacctg tctggataaa ttcgtctggg taatcacgag cgacaggatt
    34381 gatcgcttga ccatcaatat caaggtattt ttcgggaata agttctggac cgccgtctat
    34441 gggctgcccc attccatcaa agatgcgccc taccatatct tctgatacgg ctaattccaa
    34501 aggatgccct gtaaatcgaa ccttggtttt ggctaggttg atgccagttg atccttcaaa
    34561 tagctgtacc atagccttgt caccttggac ttctaagact tgacctttac gcctttctcc
    34621 attatgcaaa gtaatatcta ccaattcgtt gtagtgcact cctgcaactt gatcaacaat
    34681 catcaaaggg ccaaccactt cgctaaccgt ccgatattct ttgagaacgc tcatctaaat
    34741 tcctcccttt gctaaaacgt ggtgaatctc tttggtaacc ttctgagtaa gccctttaat
    34801 ctgctctaag ttttcttctg ggataaattt gctgcgtgcg atgcgatcgc gtacctgagc
    34861 agtgccttcc ataatctcat taaaataagc tcccaaagaa agggctttgc tggcttcctc
    34921 attaaaggtc aaaatattgg ttagcatggc ctcttgtttc ggaaaggaag taaaggtatc
    34981 caccgaatca aaggcatttt gctggagata atcctcccga atttgccggg caacagccat
    35041 ggtcaaacga tcttgttcag acagtgaatc aagccccacc aagcgtacaa tttcttctag
    35101 actagcttca cgctgcaaaa tagccatggc acgagttacc ttgttggacc aattagattc
    35161 ctgtttacgg tcaatatagc ttcctacatc atcttgatac aatgaataag acgtcagcca
    35221 gttaatcgct gggaagtgac gccgttgcgc aagaggcgcg tcgagccccc agaaaacttt
    35281 gacaatccga agggtgtttt gagtgacagg ctctgaaata tcccctccag gaggagaaac
    35341 cgcgccgatg gctgtaatgg ttccctcacg ttcttgactt cccaaggtcc gaacacgacc
    35401 agcccgttca taatattcgg caatacgact ccctaagtaa gccgggtagc cttcatcacc
    35461 aggcatttct tgtaggcgtc ctgacatctc gcgcagagct tctgcccaac gtgatgtcga
    35521 gtctgccatg atagccacag aatagcccat atcacggaaa tattcggcaa tggtaatgcc
    35581 tgtgtaaatc gacgcttcac gcgctgctac tggcatatta gaggtgtttg caattaacac
    35641 cgtgcgctcc ataatggact ggcctgtatt tgggtcaatt aactctggaa actcattcaa
    35701 aacgtcggtc atctcgttgc cgcgttcccc acaaccgaca taaataacaa tatcaacatt
    35761 ggcaaattta gctatttgat gctgcacaac tgtttttcct gccccaaatg gtccaggaac
    35821 agcagcggca ccaccttttg taacagggaa aaaggtgtca ataacccgtt gacctgtaac
    35881 caaaggttcg actgggataa gcttttgagc aacagggcga ctttgacgaa ctggccaagt
    35941 ctgcatgagg ctaccttggt aaatggaacc gtcttcctgc ttaatttcat aaactgtatc
    36001 tgttactgtg aagtcacctg catgaatagc caccaaggtc cctgaaactt tataaggaac
    36061 catgatacga tgctcaatga cagctgtttc ttgcacagtt cctaaaatat cacctgcaac
    36121 tacttcttga ccaacactta gcttgggaat aaatgcccac ttagcctttc gatcaagact
    36181 tgggatagcc acaccacgga ttaaaaagtc gctgtccgtt gctttttgaa aacgatcaag
    36241 cggtcgctga ataccgtcaa acatttctga aatcaggcct gggcctaact cgaccgacaa
    36301 aggacaacca gtagtcacta ctggttctcc tggaccgatc cctgatgttt cctcataaac
    36361 ctgaatagac gcttgatcgc ggcgcatttc aataatttct ccgactaagc caagatgtcc
    36421 cacacgacaa atatcttgaa tattagcttc ttgcatccca gaagccacaa caagaggtcc
    36481 cgaaacggtt attatttttc cttggttcac ctttttcctc ctttattttt tccaaatcat
    36541 actaactaat ctatactgtc aaggtttaat gtgactattt tgaatagtca gcaaactatc
    36601 ctatgcgttc agctgctaac ttcaccaatt ttcctgtcct tgcatttcat cagttagcta
    36661 ctcataagcc tcaaacagag tataagtcct ctttgcctaa cgtcttagtg ttataaaata
    36721 ttttgaccga cagccttttc aaccatggct tgtatgcgtg acaggcctat cccttgcgct
    36781 ccttgatgag ttggtaatgg tatgactgct ggtagaactt gattgtcata gtgagtgagg
    36841 gcttctggaa tagctgcagc aatatcttct gtgatataaa taataccaaa gtcttccatt
    36901 gctagttgac gcaactggtt aatagcgtct tgtggtttta ttactggaaa agtttgaaac
    36961 ccaatcatct gaaaaggtaa aataacatct ctgttaccaa ttaccccgac tttatacaat
    37021 gttttggtca tgctattggt ctcatccttt ctatgacacg ttctttgggt aaatcattag
    37081 ccaaagcagc cgctaaaagc cgtagatttt ttacttcaaa cgatttagcg agcaaaaagc
    37141 gagcaagcac ataaggtcca tcgacttgat atttagcctt cgcaaataaa tggtaaaggc
    37201 attcatcaac aaggtattct aactcagtcg tttgaagagt gccttggcga agtttctctg
    37261 aataaggttt tagggctgtc atatagctat caggtgttac attttcaaac cacgtcatca
    37321 gatccttatc atcttcaagt gtgataaatg ttttacttgg tctacttgct tcatcagata
    37381 ataactgcat catgaaactt attggtttat tttgcgattt ggcccgtctg acggtaatca
    37441 gattatacaa atcaatcaca ataagaacag cttgttggaa aacagggtcc tctaatcttt
    37501 gtgctatttg tttcaacgcc ttaaaataag ctaagtcagt accaatttct aagacgcgta
    37561 tgtcttgata atcttgatat tcggcccaaa tggactggat ttctgttacc acctcttttg
    37621 gaaactcatc agaagtcatc gtcctaatca aatgttccaa agccaccaaa ggcttgtcac
    37681 caattggtag taataagtga ctcagatctg cttgactagc tttagctttt aacaacactt
    37741 tgacattatg ataggtgtaa cgcaaggtaa ataattgtac tatgtcaggt tgaggggttt
    37801 ctgcaaaagc ccagcgatag gtcttggtta gctcagccat taagatagat tctagccgat
    37861 ccaaatcgtt gagatcgtca acagttaggt gatagactga ctggtgcaac agtcgagcta
    37921 aggttgttgt attgggagcc tgcaatagct tatcaaattg ctctttagtc aataactctt
    37981 tttctttgac actgatagta gtgtttaatt ctagaaatgt tggcatcgct agtcaaacct
    38041 cctatatctc tgaaaaaagc atatcaatga tgcgggagct ttcttcttta agaactgatt
    38101 ctaaaaggta acggtataaa taattgtcat cgacttggtc taaactgact aaaaatcctg
    38161 cttcctgagg aattagctct tgattatagc ttaattgagg aaacgctaat ctcagctcag
    38221 cacaatcata agaactgaac ttttgccctg ttttttcgcc aaaagtaact tgcaatggct
    38281 gttctggata tttagacaag acttgcttca aaaaagccaa ttcttcttcc ttagaaaagt
    38341 tcgtcatctt ctccaaagaa gcttcaaata gctcctttat actgtcttgt tttaatgcta
    38401 gtagagcttg tctttcttgg tttttgagtt gctgctgcgc aacctgaaat tgctgttcaa
    38461 gagcttttaa ctctttttga cgctttgctt ttaaatcgtg tagtccttgc tgttgacgct
    38521 ctttaaagtc agtatcaaga ctatctgtcg ctatctttag gcactgttga ccttcttgat
    38581 gtgctttttc aagaacattt tgtcttaact gtgttatatc attcacctaa tttccccctt
    38641 ttgatagtgt tagtggtgcc accgtttttt aaaatctaga taaggaccgt taccttatcc
    38701 aattttattt aagagaataa aggagacgac aaaggcaaga atagcatagg tttcaaccat
    38761 agctgctaaa atgacaccct tcataaaatc tttagggcgc ttagccaaga tttgcatacc
    38821 agctacagaa acattgcctt ggtgcttggc cgaaaaataa ccaacaatag caattggaag
    38881 agataccaaa aagtaagcta atccttggtt aactgaaagt tctggtgtca gtttcatcca
    38941 aattaaaata ccaatagcaa agccgtagat tccttgacta ccgggtaaca gttgcaagat
    39001 aagcgcagac gtaaattttt cgggttcttc ttttaagagc gcagctgccg cttgaccacc
    39061 tttaccgaca ccataagctg agcccatgcc actaagagct acggctaaaa caattcctaa
    39121 ggctgcgaag aaaacgccac catgtgctgt aaaataatgt gctaaatgtt ccatgttaat
    39181 tgtcctccaa atctgtttct tcattgacat tgacataatt atcagctaac tttaaaggat
    39241 tgaaagcttt tcctccacct tcataaaatt ttccaaaaaa ctctacgaaa ataagacgag
    39301 caccatgcac atatcctgaa agcattgata agaaaatatt gatcgcatgc aaaagaataa
    39361 aaataaagat acctactgta aagcgcgtga ctggtggaaa aatcccaaca atcatgttaa
    39421 aagccgctcc aatactagct ccagaaagcc ctaatgccat tagacgagta aagctaacca
    39481 aatcacttaa gtaagagcta atgccatata aattatataa tccactacct agacctaaaa
    39541 ggcttttgct ttttagtaaa gaaacaacca aaatcccaaa ggcattgccc aatgcaagcc
    39601 acttaccaat aaccgataag taagcaaggc cagatacatt cttaccaaga ataaataaca
    39661 gcaggcctaa taaaataaaa gtccaagcta gacttgacgt ataggccgta gcatatgctt
    39721 tcatccttac ttgttgccat gctcctaaca aaagcccaaa tataagtgta acaaacccaa
    39781 atagcaaaga aacaaccaaa atagtaatca catccgtttt agttgataat aatgccacag
    39841 gtaagtcaaa accaaagaaa gaaccgtaaa taagccccca tatggctact gaaatagcta
    39901 aaatattaaa gaaggtcact aaccgtttac tggttttttg gagattaaaa aagaccaatg
    39961 cagctagtgt cacagcatat agcaacaaac catagcccaa atcagctacc atcatgccaa
    40021 aaaaagtaag gtataaagga gctaaaaaag gtgtcggatc tttttcttga tattttggca
    40081 aagcgtacat ttctgtaacc aattcaaacg gtgcaatgta gcggtgatta cgtaacttaa
    40141 tgggaacatc ttcccaatcg tctggcgtga catcgtaact atccagataa aacatatcac
    40201 ctagtgtctt agtcattagc ccttttaatt ggttaaccga atcagcttct atccaccctt
    40261 ctaaggctat tagatgtctt gtaccgagta attgcttttt agtttgctgc cgttgatact
    40321 gactaaggac atagtctatt tcaacctgta actgtgccag tatcttttga gactgcctaa
    40381 gctcttttag tagactatct ttttcagcta gccaattcgt tagtaactct ttgtttatac
    40441 gaagttgttc tgatggcagt agctgttctt tatataataa gggtttaaac tgatatttct
    40501 gtaaatgaat cgtatcttgt gcttgccaaa ataagacaag gccgtactca aattctgtat
    40561 ggtatacttc ctcaataaaa agacctttgt gctgctttag ctgatgataa aaatgatcat
    40621 ttgctgtact aggaatcgtt ccaactcttg ccgacaaaaa atgaaattgc gctaagtcct
    40681 gaggcaaaac agtcaaggaa cgccactttt ctaactcctg acagtattct gttagctggt
    40741 ctattttgtt ttgcagctgc tctagtcgtt ctatcttttg tgacatcatt ttcaaagccg
    40801 tttgagctgc ttgttgtcga tggcgttctt cgaactgttt aaacgaaaga gatggcgttt
    40861 tttgtctcaa ggctgttaac tttcctattg gtggtaaaaa cgctgataag ctagcaaggc
    40921 cgttttctaa ctcttgttgg ctttgtaaaa ggtattgtaa agcttcatca tctagtagag
    40981 gtttttgatg cgtcagatcg tattggacga tttgtggcaa cttgacgttg cctttgttaa
    41041 aagcatcttg ccaatgtttt agttgcttca tgtcacgaac ttcaactaat tgagattgtt
    41101 gaagtgtttt taaaacaaga tcaagataat ctttttcaaa aaccattgcc agctttttca
    41161 tttgactaat ggccatatgt ctccaccacc ttatccacta actccctaac aacggttagc
    41221 ttatgctctt ctaaaatggc atgaagggct agctgttgcg tcgctaacct cttttttgaa
    41281 gctttttgtt cttgacgatt agcttcaaaa aacacctcac gtttagcttc taatagtagc
    41341 tgagtttctt gttcgtactc aaggaacagc tgctttaact cttcttcttt tttgtcagct
    41401 agagcttttt tcttagcatc gtaactgtca taaattgttc tagcctcttg ttcgatacta
    41461 ttcattagtg acaatagggt attcatcaca tatcaactcc ttatctttat ctattgctaa
    41521 ttctttgact cactaggtac ttaaaaacaa agctacaaga aaggctactt ttgggggtta
    41581 atcaaccagc gactataagc tagagctaaa tctctaacct atctactaac agcttttttg
    41641 agataggata attgcttgat taagcgaaca gtatcttcaa gttaagcgtt ttcaattcta
    41701 tccataaaaa caatggctta gttcagaaaa aggttagcta aaaacctgtc aaagcagtaa
    41761 gatgacacag aactctaaag ctttgtattg ctgtctttcg tgtattatct tatacttctt
    41821 taggcattta gtaaccttga tctagttgac ggtaaggaac ctttaccagc caatagccaa
    41881 gccaatgtga taacagtata actgctatcg tgttaaaagt cctgtcaaaa agatgtttat
    41941 tagtttctcg cttgtaattc ttgttttgtt actaaacggt aatttttaaa gacagaacct
    42001 ccaatataac ccacaattaa gctgataata atacatccaa gggctgttat caacacttta
    42061 gcgacaggat tataggcaaa catcaccgca aaaccggcaa tcggagtagc tgtccctggc
    42121 gtatcattaa ccaatttcat tagggcgatc aaaacaccac atgcagcacc accaacgaaa
    42181 ttagtcacat aaatcggaat gggattggct gacgtcacat ctgcctgagt caacggttca
    42241 atagcaaaag caatattgtc ttttctttca cccaatttta atcggtaaaa aagaacacca
    42301 ttcataaaag aagagccaaa gacagataag gcacctatag ccatcggaat acctgtcaga
    42361 ccaagcatgg ctgtcaaagc cattgatgac agcggagctg ttgctacaac aacaatcgta
    42421 ccgcctagta tcattcccat gataataggg ttgctattag cacttgaggt caggatgtct
    42481 ccaatagtca ataaagtact attgatcact ggcgtaatta actgcgccaa gaaacgagtt
    42541 aatggtgcac cgacaataat aatcgagatt aaatcaagcc caccaggaat atttttttcc
    42601 atccacttga ttaaaaaaga taccacatac ccagccacaa aacctgggat aatacctgtt
    42661 cctgaaactg acaaaccaat caaaacggca taacctggag acacaccaat tgcaatggca
    42721 accaagatag cagcagctac ccctgataat gagccatttg ctccgcctac ttcacttaga
    42781 aactgaaagc ctaaaacctt accaaaaaaa gaatcttgaa aggcttcaac taaaaaagag
    42841 gcacaagctg ccgaagccaa agctcccatt gccttggcac catgaggagc cttatagtta
    42901 aacaagctaa aaatagctaa aacaagaatc aaaagacttg ttccaataat aatatccata
    42961 atctattcca ccaatctatc tcattacata agacactata actgcttcac tctggcaaca
    43021 gcttcgactt caatcaaagc ttgcaaagga agcgccgaca cttgaaccgc tgtccgaaca
    43081 gggtaaggct cttcaaacaa atcagtcatg acatgattta atatttccac attggtcaca
    43141 tctgttaaat acacatttag cttatagata tgattcaagt ccaacttttg ctctgccaaa
    43201 atagattgta aattaacaaa aacctggcga cactgtgcct caaaaccatc tgacaattga
    43261 cctgtcactg gattaagggg taattggcct gcggtgtata gaaaatgtcc ttcaatcgta
    43321 taagttgaat aagggcccat tggttctggg tagctcttca tatacgaacc tcctcattga
    43381 taatatagtt aaattttaca ggaaaccgca ttcacagtca ataataaatt ctctttttga
    43441 taaaaaatta ttattaaggt gattggcaat tgttttttag tctaaaatgt attactatat
    43501 ttgtaaataa gaagatagat ataggaagtt tgacatggat aaagaaacgc taaactactg
    43561 gaaaacggtt atcacatttc ttcacgatgt cttaggtgat aactatgaaa ttattttaca
    43621 tgtgattgat aaaaatgata tctatattgg agaactcgtt aatagtcata tcagtggacg
    43681 gtctaaacag tctcctctca ccacctttgc tcttgatttg attaccaata aagtctataa
    43741 ggaaaaagat tttgtgacca attacaaagc aatcgttagt cctcagcaca aagaagttcg
    43801 gggatctact ttttttatta aagataaaaa gggtaatctc gagggaatgc tatgtattaa
    43861 cttagatatt tcagcctatc aaggagtagc taaggacctc ttaaaacttg tcaatctcaa
    43921 tttagaacat ttcatcccaa ctgctaaaga gcctaaaaca gtcacacccc agccagaaga
    43981 agctgttgaa attttaacct ctaacatcca agatattatc ggtcaaatta ttgatccctc
    44041 gttactgaga cacaatgtac atcttagcca agatgtcaaa atagacattg ttgccaagct
    44101 ttatgaaaaa ggagtctttc aactaaaagg agccgtctca aaagtcgctg atattctatg
    44161 tatttccgaa ccaagtgttt accgctacct taaaaaaatt gaagccgatc aataaccaaa
    44221 tcaccataaa acgccagtta aaagtatttc agataagcat cacaatctct gatataccga
    44281 tgactgacgt tttttttact caatatgcct ttatggcaag aaaaggctac tggtcattaa
    44341 ccttcagcta tgcttagagg tacacctgtc gcagcttgaa cctcatcaaa agtaaaccct
    44401 tcactgattt caattacttg gataccatct ggcgttactt taaacacacc catttctgtg
    44461 ataataaggt caaccacacc ttttgctgtt aaaggtaagg tacattcttt taggagctta
    44521 tgctttcctt tattggtatg ctccatagct acgatgactt ttttagcacc aactaataga
    44581 tccattgctc ctcccatacc aggaaccatt ttgcctggaa tcaggtaatt ggcaatatct
    44641 ccattttctg caatttctaa agcacccaag acggtcaaat caacatgacc accacgtata
    44701 atgccaaaag aatctgcact attaaagaaa gcggctcctg gataaaccgt cacaggctgt
    44761 cctcctgcat taacgatagt cgaattcgga tcatctgact taggacctag tccaataaac
    44821 ccattttcag attgaaaata cactgtcatt tcttcaggca caaaatttgc tactttggtt
    44881 ggtagaccaa tacctaaatt taccaaagta ccatcttcta attctttggc tacacgcttt
    44941 gcaatacgat tttgaatctc ttctttactt aatgcaaccg ccataactat actccttctt
    45001 taacaatgta atctacaaaa atgtgtggtg tatgaacgca ttctggttgt atggtgccga
    45061 ctggtactat ttctcgcgcc tgaacaattg ttgtttttgc acaagctgcc atcaattgat
    45121 taaaattatt ttcagaacca ctatactgta aatttcccat ttcatcagct ttattagcaa
    45181 atatcaaggc gacatctgcc tcaaatgggt attctaagag ataggttttt cccttgatgg
    45241 tttttgtttc cttgccttct gccactaagg ttcctacccc tgttggcgtg taaaagccac
    45301 caatgccata agctccgatg cgaatttttt cggcgaaggt tccttgagga ataagctcaa
    45361 tcgtcgtttc accggcagtc atttggcgcc cagcttcttt atttaatcca atatgtgttg
    45421 cataaatggt tttgaattga tgattagcca ccatttttcc aacaccgcga tcaacaaaac
    45481 cagcatcatt acagatcaag gttaagtctt ttgtgccttt gtcaaccagt gcatcaatta
    45541 acgcttctgg tgttccatta gccataaaac caccgaccat aatcgtgtca ccatctttaa
    45601 tatgactgat agcttctgct attgctattc ttttattctc tttcatacgg ttaccttcta
    45661 tcttgttacg atgatacttt gaccttggcc accaccaata caaagcgttg ctaagccatg
    45721 tctcacatca cgcttagcca tttctgctaa taaggtgact aaaatacgag cacctgatgc
    45781 tccaatcggg tgacccaaag caatagctcc tccattgaca ttaacttttt cattgtcaaa
    45841 accaaggtct cgtgaaaccg ctaaagcctg agcagcaaaa gcttcgtttg cttcaatcaa
    45901 atcaatgtca tcaattgtca gctgagcctt tgcaagagcc tttttcgtag caggtattgg
    45961 tccgcagccc ataatacttg ggtctacacc tgcacttgca taactagtga ttttagctaa
    46021 aatagggagc cctaattctt cagctttttc tttactcatc aataaaattg ccgctgctcc
    46081 atcattgatt cctgaagcat taccagcagt tactgtcccc tcatctttga taaaagcagg
    46141 gcgtaacttt gctaacttat ccactgtcgt tccaaattta gggtattcat cttgatcaat
    46201 gagcaaaggt tcacctttac gctgaggaat agtgacaggt gcaatctctt ctttgaaacg
    46261 acctttttct atggcaagtt gggctttacg ttggctatca gctgcaaagg catcttgttc
    46321 ttctctggtc agtccatact gttgcacaat attttcggct gttataccca tgtgataacc
    46381 tgcaaaagca tcactcaacc catctttaag catggtatct actactttac tatctcccat
    46441 acgagatccc caacgttggc cttgtaaaac gtaaggtgct tgactcatgt tttcggtacc
    46501 accgactaaa acaatatcag cgttgcccgt ttgaatcttt tgagcaccta actcaactgc
    46561 ttttaagcca gaaccacaga ccatatcaat cgtaaaagca ggggtatcat aagaaagccc
    46621 cgcatttagc gctacttgcc gagcaacatt ttgacccaga cctgcatgta acacattccc
    46681 aaagatcact tcatcaatgg catcactttt aagaccggta tcagctaaaa tttttgtaac
    46741 aactgtcacc cccaaatcta cggctgataa agatttaaag actcctccaa aatttcctat
    46801 cggggtacga taagcagatg tgatcaccac ttctttagtc atagaaactt ctcctttttc
    46861 attattgaaa cgcttacatt tttattatat aatgtgaact ataataaaca aatatcgttt
    46921 ttttatatat gctataacta aacgttatcg aaagaggaaa atatgaatat ttcaagcttg
    46981 gcttatttta ttgagattgt taattgtgac tttaacttaa ccaaagcagc ccaaaaaagt
    47041 catatttcgc aacccgccct tagtagttat atcaagacta tcgaaaaaga agaaggagtc
    47101 gttttattcc agcgtcaaaa agggcgtcta gttggactaa cctcagtcgg cgaacgcttt
    47161 tttgaaaatg ctaaggtggt tatcgcatca catgagcaat taatggctga tttgcggagt
    47221 gaatccaaaa aatacaaagg agaaatttca atcggtattc ctcaactcat ctctggcatt
    47281 gttttttctg agctcattcc aaaactcatc gaagaaaatc ccgaagttaa atttcatatt
    47341 gtagaaacgg gtgcttatga actgcaaaaa aaactgtttc taaaagaagt cgattttgcg
    47401 attttattaa cacccactac actagatgct actattgctg atatcgacat tctcctaaga
    47461 gatcaactct gcctttatat gagtcacaaa acctttcaag agacgtttga tagtaatcct
    47521 aacatctcta tcaaagcatt acatcgtcaa aacttagtaa cttttgataa aacctttatg
    47581 attaatcatc agcttaccac cttatttgaa gaagcaaata gtcttcctgt agtcaaatgt
    47641 tattctgcct cgtgggattt tttattaaat tgcacgcgct attcctctta cctaacaatc
    47701 ctaccaagac ctattactca ttttgctcat atggacggtt tagtggaggt ccaactgacc
    47761 gaacatccaa aatgggaagt tgttctagcc tctctcaagc acaataaaac aagccattta
    47821 aaacattata tcaagcacac catcttagat tatttcatga accagcgctt ctaattgctt
    47881 gactcagacc ttaaaaaaaa cgcctgaagg aaacaatccc aagcgtttca tgtagttatt
    47941 tcctaaaata caaataggta aatgatggca atacctgtga ccaccaagac acaaggaata
    48001 gtataagcca caatttgatt gaattttagt ttgaagtttg agagataaga taaggcccaa
    48061 aaaggaacta tcaagtttgt tgtggcatct cctaattgat aagcctgcaa aatcttacct
    48121 aggtcattac caacttcgat agttgcaggg acgatatagg gagcttcaat cacaaatttt
    48181 gagcctcctg atggtaccgc aatattcagc aaagccgagt aaagataagc aaagaccggc
    48241 catgttgtcc catttgaaat agcgacaaag aaatgggaaa tctctagtcc tagacctgta
    48301 ctttgaatga ttccaaaaat accagcgtaa aatggaaact gaagcaccaa gccccaagat
    48361 gacatgaccc catctttgaa taaggaacca taatattctg gtccttgttg accacaaaga
    48421 agcaacccta agcctaaaaa taaaaagtta aaactattga gatctagacc agacaagccc
    48481 accaccaaaa aatgataaag gctatagcta acaatggccc caccaacaag atacatcact
    48541 aagcgagatg cgttcatctt ttcagcaggt gtgtttcttg caataacaac atgtgaagcg
    48601 gtatcaagac tatggtgata gatctcatca tcaagttcca tgattttagt ttcatctttt
    48661 ggatgcgcca ataaagcaaa gcaaagcggt accagcatac acaccactaa aagactgata
    48721 aatggaagat tcaaaactga ttctgtcaga ggaacacttt caggaacaac ctgtttataa
    48781 gcatctgcga cactatttcg caaataatct ggagtagccg aataaagcac cgcagcacca
    48841 gataaaccag aagtagctgg taaaaaggta aaaaataaag tagcaacaaa caaaggtgta
    48901 tgcaccttaa tccctttttg cctagcctga acaagcaact ctttcccaaa cacaatggca
    48961 accatcatac caagacccca gtgtaaaaag ccaaagatag agcctactac aagagaaaaa
    49021 ataaaaatct gagtcctggt ttgaggcagt ttggcaaggg acttaaacat cttatgagca
    49081 ggtggagaac tcgctacagc attccctgtc gcaagtaaaa tagtcatctg catggcaaag
    49141 gacaacagcc cccaaaaacc atttacccag ccatagataa tcccgcccgt cttttccgta
    49201 ccaataaagg gactatccgt catcaacaca gacatagtaa cgactaaaaa ggttaaaata
    49261 aaacagataa aaagcgattc tggcatccat ttcataaagc catcaatata gcgatccata
    49321 aacctttttt tggttttgat gttattcatg ttgtcaattc ctttcacatt gtccacaagc
    49381 acttttgttc ttttgatcag taacctgtcg ataatactaa taagcgttta tttagttcat
    49441 ttagttatca ataagtttaa acccacaaaa aacacttttg ttcccttttt attatgacaa
    49501 cgttttcata aacaaacaac tatgattttt ttattgcttc cataaccctt at
//
LOCUS       DAUGFP010000009        46294 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.009, whole genome shotgun sequence.
ACCESSION   DAUGFP010000009 DAUGFP010000000
VERSION     DAUGFP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46294
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.009"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            15..95
                     /locus_tag="VH663_000512"
     tRNA            15..95
                     /locus_tag="VH663_000512"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Tyr,seq:gta)
     gene            102..172
                     /locus_tag="VH663_000513"
     tRNA            102..172
                     /locus_tag="VH663_000513"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Trp,seq:cca)
     gene            184..256
                     /locus_tag="VH663_000514"
     tRNA            184..256
                     /locus_tag="VH663_000514"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:217..219,aa:His,seq:gtg)
     gene            265..336
                     /locus_tag="VH663_000515"
     tRNA            265..336
                     /locus_tag="VH663_000515"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Gln,seq:ttg)
     gene            356..439
                     /locus_tag="VH663_000516"
     tRNA            356..439
                     /locus_tag="VH663_000516"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:390..392,aa:Leu,seq:caa)
     gene            1522..1830
                     /locus_tag="VH663_000517"
     CDS             1522..1830
                     /locus_tag="VH663_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ8173740.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSDVPYCLLSGCAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST
                     "
     gene            1885..2097
                     /locus_tag="VH663_000518"
     CDS             1885..2097
                     /locus_tag="VH663_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ8173741.1"
                     /translation="MVAAIEAGNYNDGILTEMNNLLEDIFIAKRPFIQKIKEKTLQAG
                     AATALMIGSGKTVFALCQTEKQTIIV"
     gene            2207..2650
                     /locus_tag="VH663_000519"
     CDS             2207..2650
                     /locus_tag="VH663_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEQ8173742.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            2654..3373
                     /locus_tag="VH663_000520"
     CDS             2654..3373
                     /locus_tag="VH663_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173743.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRRLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEVETDDEKGGHGHA"
     gene            3366..4181
                     /locus_tag="VH663_000521"
     CDS             3366..4181
                     /locus_tag="VH663_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ8173744.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVIGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(4221..4604)
                     /locus_tag="VH663_000522"
     CDS             complement(4221..4604)
                     /locus_tag="VH663_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ8173745.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWEGITINQENWDLAPFLKHL"
     gene            complement(4655..5911)
                     /locus_tag="VH663_000523"
     CDS             complement(4655..5911)
                     /locus_tag="VH663_000523"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEQ8173746.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHSEEVYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            6003..8315
                     /locus_tag="VH663_000524"
     CDS             6003..8315
                     /locus_tag="VH663_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ8173747.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQKHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEISVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            8579..12145
                     /gene="rpoB"
                     /locus_tag="VH663_000525"
     CDS             8579..12145
                     /gene="rpoB"
                     /locus_tag="VH663_000525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ8173748.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            12236..15877
                     /gene="rpoC"
                     /locus_tag="VH663_000526"
     CDS             12236..15877
                     /gene="rpoC"
                     /locus_tag="VH663_000526"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEQ8173749.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDNQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTADKYFLEPGQDIQEVIDGLEINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAVFTAEAE"
     gene            16029..16394
                     /locus_tag="VH663_000527"
     CDS             16029..16394
                     /locus_tag="VH663_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEQ8173750.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
     gene            16487..17425
                     /gene="tadA"
                     /locus_tag="VH663_000528"
     CDS             16487..17425
                     /gene="tadA"
                     /locus_tag="VH663_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEQ8173751.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGHES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYARLLELGVTKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            17409..18395
                     /locus_tag="VH663_000529"
     CDS             17409..18395
                     /locus_tag="VH663_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEQ8173752.1"
                     /translation="MKKLSSKHQHKFIQLLANLLSTGFSFTEVIAFLKRSQLLQLDYV
                     LKMEESLLKGQGLADMLSGLGFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRR
                     KTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLNHFPAFFIGFCSGLILL
                     FGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMAILDI
                     MAIEKSSLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELE
                     IYAQESWEQFFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILLPIYQNMGGIF"
     gene            18397..18723
                     /locus_tag="VH663_000530"
     CDS             18397..18723
                     /locus_tag="VH663_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEQ8173753.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            18698..19126
                     /locus_tag="VH663_000531"
     CDS             18698..19126
                     /locus_tag="VH663_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEQ8173754.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            19131..19367
                     /locus_tag="VH663_000532"
     CDS             19131..19367
                     /locus_tag="VH663_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ8173755.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEITLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            19360..19794
                     /locus_tag="VH663_000533"
     CDS             19360..19794
                     /locus_tag="VH663_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEQ8173756.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRNHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            19778..20104
                     /locus_tag="VH663_000534"
     CDS             19778..20104
                     /locus_tag="VH663_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ8173757.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVSFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            20202..21155
                     /locus_tag="VH663_000535"
     CDS             20202..21155
                     /locus_tag="VH663_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ8173758.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVTLITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            21214..22410
                     /locus_tag="VH663_000536"
     CDS             21214..22410
                     /locus_tag="VH663_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEQ8173759.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEAVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEHIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGDISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            22597..22905
                     /locus_tag="VH663_000537"
                     /pseudo
     CDS             22597..22905
                     /locus_tag="VH663_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22988..23758)
                     /locus_tag="VH663_000538"
     CDS             complement(22988..23758)
                     /locus_tag="VH663_000538"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEQ8173760.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLT
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(23806..24873)
                     /gene="pepA"
                     /locus_tag="VH663_000539"
     CDS             complement(23806..24873)
                     /gene="pepA"
                     /locus_tag="VH663_000539"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEQ8173761.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPNIEDIVFDGGFTDKAEAESFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKRY"
     gene            25329..25622
                     /locus_tag="VH663_000540"
     CDS             25329..25622
                     /locus_tag="VH663_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEQ8173762.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            25619..25936
                     /locus_tag="VH663_000541"
     CDS             25619..25936
                     /locus_tag="VH663_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEQ8173763.1"
                     /translation="MIRPTSYESLAALIEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFVCVNRDQFIEVAQKWNIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            25954..26580
                     /locus_tag="VH663_000542"
     CDS             25954..26580
                     /locus_tag="VH663_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEQ8173764.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEASSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPIVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELALPNAPQKRGIIELDESAVVGEAFDPAKHWKG"
     gene            26732..27127
                     /locus_tag="VH663_000543"
     CDS             26732..27127
                     /locus_tag="VH663_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ8173765.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISVVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(27381..28022)
                     /locus_tag="VH663_000544"
     CDS             complement(27381..28022)
                     /locus_tag="VH663_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEQ8173766.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYEVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(28042..29019)
                     /gene="dusB"
                     /locus_tag="VH663_000545"
     CDS             complement(28042..29019)
                     /gene="dusB"
                     /locus_tag="VH663_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEQ8173767.1"
                     /translation="MIKLNSSFRIGDVEIPRRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFETVR"
     gene            complement(29006..29878)
                     /gene="hslO"
                     /locus_tag="VH663_000546"
     CDS             complement(29006..29878)
                     /gene="hslO"
                     /locus_tag="VH663_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEQ8173768.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISYLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNESDLEAIINDKA"
     gene            complement(30025..31518)
                     /gene="rofA"
                     /locus_tag="VH663_000547"
     CDS             complement(30025..31518)
                     /gene="rofA"
                     /locus_tag="VH663_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEQ8173769.1"
                     /translation="MIEKYLESSIESKCQLIVLFFKTSYLSITEAAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALVPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSRDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHIRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFNLQHFIPE
                     TNLFISPYYKGNQKLYTSLKLIVEEWMAKLPGKRYLNHKHFHLFCHYVEQILRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSINFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVQHELTKGIAVAEISFDESILSIQELIYQVKEEKFQADLTKQLT"
     gene            31750..33606
                     /gene="sfbI"
                     /locus_tag="VH663_000548"
     CDS             31750..33606
                     /gene="sfbI"
                     /locus_tag="VH663_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein SfbI"
                     /protein_id="HEQ8173770.1"
                     /translation="MVSSYMFVRGEKMNNKMFLNKEAGFLAHTKRKRRFAVTLVGVFF
                     MLLACAGAIRFGQVAYAADERTVPNFKSPDPDYPWYGYDAYTGAFLRYHDLRVNLNGS
                     RSYQVYCFNIKKNYPRPFTSSDKKWYKRLEGTAETFKVHAMAPRVGGEELTKKLRSVM
                     YNGYPNDGNNIMKGLEPSNAIEVTQYAVWYYSDKSQYEIETLWKSEAKEGKISESQVT
                     LMREALRKLISSDEDLVKQVPSNFKLSTFESSDKSYQNLLSAEFVPDDPPKPGDTSEH
                     NPKTPELDGSPIPEDPKHPDDNLEPTLPPVMLDGEEVPEVPSESLEPALPPLMPELDG
                     EEVPEKPSVDLPIEVPRYEFNNKDQSPLAGESGETEYITEVYGNQQNPVDIDKKLPNE
                     TGFSGNMVETENTKEPEVLMGGQSESVEFTKDTQTGMSGQTTPQVETEDTKEPGVLMG
                     GQSESVEFTKDTQTGMSGQTTPQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGQTA
                     PQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSETVTIVEDTRPKLVFHFDNNEP
                     KVEENREKPTKNITPILPATGDIGNVLAFLGILILSVLSIFSLLKNKQNNKV"
     gene            33894..34742
                     /locus_tag="VH663_000549"
     CDS             33894..34742
                     /locus_tag="VH663_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEQ8173771.1"
                     /translation="MTERLKNLGILLLFLLGTAIFLYPTLSSQWNAYRDRQLLSTYHK
                     QVTQKKPSEMEEVWQKAKAYNARLGIQPVPDVFSFRDGIHDKNYESLLQIENNDIMGY
                     VEVPSIKVTLPIYHYTTDEVLTKGAGHLVGSALPVGGDGTHTVISAHRGLPSAEMFTN
                     LNLVKRGDTFYFRVLNKVLAYKVDQILTVEPDQVTSLSEVMGKDYATLVTCTQYGVNT
                     KRLLVRGHRIAFHYKKYQQAKKAMKLVDKSRMWTEVVCAAFGVVIAIILVFMYSKMTD
                     KKVIKN"
     gene            35028..37289
                     /locus_tag="VH663_000550"
     CDS             35028..37289
                     /locus_tag="VH663_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HEQ8173772.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMLLVLIGFEGKV
                     RAAHELVEVPVPIFHNPDPQSDYQWYGYEAYTGGYPKYDLFKTYYHDLRVNLHGSKSY
                     QVYCFNVHKHYPRPSQSFDRKWYKKLDGTAENFDSLAMEPRVRKEELTKKLRAVMYNA
                     YPNDANGIMKDLEPLNAIKVTQEAVWYYSDNSPINPDESFKSEALANKISATQISLMR
                     EALKQLIAPDLDEKQSRRVPDGYQLSIFESENKDYQNLLAAEEVPIQPPKPGDKPPLP
                     PVQTKQTSVIIRKYAEGDYSKLLEGATLKLAQIEGSGFQEKIFDSNKSGEKVELPNGT
                     YVLSELKPPQGYGVATPITFKVAAGKVLIKNKEGQFVENQNKEIAEPYSVTAFNDFEE
                     IGYLSDFNNYGKFYYAKNTNGTNQVVYCFNADLHSPPDSYDHGANIDPDVSESKEIKY
                     THVSGYDLYKYAVTPRDKDADLFLKHIKKILDKGYKKKGDTYKTLTEAQFRAATQLAI
                     YYYTDSADLTTLKTYNDNKGYHGFDKLDDATLAVVHELITYAEDVTLPMTQNLDFFVP
                     NSSRYQALIGTQYHPNELIDVISMEDKQAPIIPITHKLTISKTVTGTIADKKKEFNFE
                     IHLKSSDGQAISGTYPTNSGELTVTDGKATFTLKDGESLIVEGLPSGYSYEITETGAS
                     DYEVSVNGQNVPDGKVMKELVKEDETVAFENRKDLVPPTGLTTDGAIYLWLLLLVPFG
                     LLVWLFGRKGTKK"
     gene            37286..37807
                     /gene="lepB"
                     /locus_tag="VH663_000551"
     CDS             37286..37807
                     /gene="lepB"
                     /locus_tag="VH663_000551"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ8173773.1"
                     /translation="MTNYLNRLNENPLFKAFIRLVLKISIIGFLGYILFQYIFGVMII
                     NTNDMSPALSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVSFTQEGG
                     LLINGHPPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            37829..38878
                     /locus_tag="VH663_000552"
     CDS             37829..38878
                     /locus_tag="VH663_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HEQ8173774.1"
                     /translation="MKKNKLLLATAILATALGTASLNQNVKAETAGVVSSGQLTIKKS
                     ITNFNDDTLLMPKATFTFDVKPVDVTGEEKDTASGLKIQKGIAGVASQTIQYDNTDKP
                     TNKEKAVNFDFSTVTFPNVGVYRYEVSEQAGDVKGITYDSKKWTVDVYVVNENNKFTP
                     KYIVSKETTDSSKKPIVFNNELKTTSLTIKKEVTGNSGDKTSDFTFTLLLKENTQFET
                     GQKVKATKKKSGAEEETVEVTIGKQYTFTLKDSEELILDKLPIGITYQVDETDKNKEQ
                     YETSAKMTEDSKEAQGYTLNDLKTTDETADTITVTNKRNLPIPTGVVGILAPFAVLSI
                     VAIGGVIYITKRKKA"
     gene            38894..39619
                     /gene="srtB"
                     /locus_tag="VH663_000553"
     CDS             38894..39619
                     /gene="srtB"
                     /locus_tag="VH663_000553"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEQ8173775.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNDFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKHHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
     gene            39636..40223
                     /locus_tag="VH663_000554"
     CDS             39636..40223
                     /locus_tag="VH663_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEQ8173776.1"
                     /translation="MRKYWKMLFSVVMILTMLAFNQTVLAKDSTVQTSISVENVLERA
                     GDSTPFSVALESIDAMKTIEEITIAGSGKASFSSLTFTTVGQYTYRVYQKPSQNKDYQ
                     ADTTVFDVLVYVTYDEDGTLVAKVISRRAGDEEKSAITFKPKRLVKPIPPRQPDIPKT
                     PLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKSKL"
     gene            complement(40382..41587)
                     /locus_tag="VH663_000555"
     CDS             complement(40382..41587)
                     /locus_tag="VH663_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEQ8173777.1"
                     /translation="MVIFDLKHVQTLHSLSQLPISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQATQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQLPPVLEHLNHIMDLVKLGNPQLLKQEINRIPLSSITSS
                     SISALRAEKNLTVIYLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRC
                     AAIIHFSESLTNKSISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFK
                     KYSNISLQHYILSTKIKEAQLLLKRGIPVGEVAKSLYFYDTTHFHKIFKKYTGISSKD
                     YLAKYRDNI"
     gene            41978..45355
                     /locus_tag="VH663_000556"
     CDS             41978..45355
                     /locus_tag="VH663_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HEQ8173778.1"
                     /translation="MTQKNSYKLSFLLSLTGFILGLLLVFIGLSGVSVGHAETRNGAN
                     KQGSFEIKKVDQNNKPLPGATFSLTPKDGKGKPGQTFTSNDKGIVDAQNLQPGTYTLK
                     EETAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISKAGSKDVSSSLQLEKSTMSV
                     VSKYGEQEKTSNLADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDQRLNPKGI
                     SQDIPKIIYDSANSPLAIGKYDADSHQLIYTFTDYIAGLDKVRLSAELSLFLENKEVL
                     ENTNISDFKSTIGGQEITYKGTVNVIYGNKSGDSNNYVKNILSNVGGSIESYNTQTGD
                     FTWYVYVNPKKQSINYSILNLWGFGRARSNTNNLETNANTSSAQLEKVEIYEVKEDYT
                     LPTSYGVDITKLTLRTDITVGNKDGFPMTKRQRIDFKDNLKDGKRFVVKVTGKTDQSG
                     KPLVVQSNLASFSTNQDYVDLKISGGNVYFQNEIALSPSKGSGSGKSEFTKPSITVAN
                     LKRVAQLRFKKMSTDNVPLPEATFELRSSTSDSQKVEAKSNEQGEVHFKDLNSGTYDL
                     YEKQAPKGYQQVTEKLATVTVDTTKPAEEMVKWEKTHPFVKVEVNKEVTIVNHKETLT
                     FSGKKIWENDRPDQRPAKIQVQLLQNRQKMSDKVQEVTKDNDWSYHFKDLPKYDDKNQ
                     EYKYSVEEVKVPDGYKVSYLRNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGSKIID
                     EDTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEFKNLK
                     KYNGTGNDIMYSVKEVTVPTGYDVTYSANDIINTKREVITQQGPNLEIEEMLPLESGA
                     SGGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGATMEL
                     RDSSGKTISTWISDGQVKDFYLMPGKYTFVETAAPDGYEIATAITFTVNEQGQVTVNG
                     KATKGDAHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKSSDVIIGGQG
                     QIVETTEDTQTGMHGDSGCKTEVEDTKLVQSFHFDNKESESNSEIPKKDKPKSNTSLP
                     ATGEKQHNMFFWMVTSCSLISSVFVISLKTKKRLSSC"
     gene            complement(45621..46286)
                     /locus_tag="VH663_000557"
     CDS             complement(45621..46286)
                     /locus_tag="VH663_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173779.1"
                     /translation="MKRCNKYLFTSLLAVSILFSSYKSVHAHDNIDEKGKVHLYWQGN
                     YYVDNYVDYTKTLVDNNNSIEWTVTFNSAKEQWVYPDFSVFLPKGVKAPKEITYEHHY
                     WDGTVDSKTRSNTQWHYDWENQKTNFNQEFDKFPGYTGWSPSLDKFYKLKNDGKFSHV
                     LVDTYGRQSHTYFSHKMVWKFKTELEEGYKDKWNKLPFIAGIKQNNPLAASFPSYKGE
                     FGE"
BASE COUNT        14512 a         8084 c         9809 g        13889 t
ORIGIN      
        1 atttaatatt ttacggaaag atagcgaaga ggctaaacgc ggcggactgt aaatccgctc
       61 cttcgggttc gggggttcga atccctctct ttccatccta aacgggcata gtttaaaggt
      121 agaactaagg tctccaaaac cttcagtgtg ggttcgattc ctactgcccg tgttagaaat
      181 atggcgggtg tggtgaagtg gttaacacat cagattgtgg ctctgacatt cgtgggttcg
      241 attcccatca ctcgcctatt ttattggggt atagccaagc ggtaaggcaa gggactttga
      301 ctccctcatg cgttggttcg aatccagcta ccccagttta aattttatat ttaaagccgg
      361 cgtggcggaa ttggcagacg cgctggactc aaaatccagt gtccgcaagg acgtgccggt
      421 tcgaccccgg ccgccggtat acttgaaaaa caaggtttgt aaaccttgtt ttttgttttg
      481 atcttaagct gtagctacgt agtacaatct cttatattac tgataaggtc tatttaatgc
      541 gcaagatttg tggtatgatg aatatgattg agtattgatg ctttatggga ttaccggtaa
      601 ggttttgaaa aaggtttgtc actactaggt gcttttttag ctatagaaag acagttatgg
      661 attgatgtat tgtaactaat gttggaggtt aatggtggtt gagttttgag atatttacac
      721 tgataatcgg gtggaaactg gtcgggtatc gggtaatgga gtgtgattct tcgtttaagc
      781 aaggagatta tcgtttggtg gttcatattt gcttgttaaa tgttagggat gagatgctga
      841 gtcagcaagg ccatgaagat aaagctgact ggccagagct ttgagatgtg acgtttggtg
      901 gaagtccttt ttggtgagat ggtacagcat atagtaatat tagatcctga ggaagaattg
      961 ggactttcat taggcttgac tggggtatga cctcacttta caattcattt cgatgatggg
     1021 tttgatgata cgtttttagt ttaagacttt gtagagttgg agaatttggt tctttatgag
     1081 gaagaagtgc aggtaattcg ttgagccagt cgtgaagata ttttaggtat gattgatgaa
     1141 ggttcgttca ttccttatct gaagtataaa ataaatttgt gctttgagat ggttggccaa
     1201 tataaagtcc atcaatctta gaagtaataa tttttgaatt ttttctgagg tttgtgtata
     1261 tttgatagtg tactgatgta agaagtaagg agtactatga tggcaatcaa tgagagagct
     1321 tctgcaaaaa tcaagttagg ccttgatact aagaataagc gatgagatgg ttaccacgat
     1381 ttatcgatgg tcatgatgag tattgatctg tgtgactatg tgacggttga ccatatagat
     1441 gacaataaga ttgtctttgc ctcaaactgt ccgaaaatac ctattaacta agataatgat
     1501 gtttataaga tagttcagct aatgaaacat cgatttcaag ttaaaagagg agtttccgtt
     1561 tacttggaaa agcggattcc catgtgtgct ggtatgggag gaggttctag cgatgctgtt
     1621 actatcagag cattaaatca gttatggctc cttactctta gccgaaaaga tatgatggat
     1681 attggtatac cgattggtag tgatgtgcct tattgcttgc tttcaggttg tgcccaagtc
     1741 actggtaaag gtgaagtggt ttgtcgcatt ttggggctgt tatcgtcttg ggtggttttg
     1801 gttaaaccag attttggtat ctcgacttga acattttttc tagatattaa ttgtaaaatc
     1861 atttcaagag tgtctactac tcatttggtt gcggcgattg aagcgggtaa ctataatgat
     1921 gggatattga ctgaaatgaa caatttgctg gaagatattt tcattgccaa gcgacccttt
     1981 attcaaaaaa taaaagagaa aacactgcaa gccggtgctg ctactgcttt gatgatagga
     2041 agtggaaaga ctgtttttgc tttgtgccaa accgaaaaac aaactataat agtttaaaag
     2101 gactttgcaa ggaagttttt aaggtgagaa tgttgtaata ttttgttggg attgcttctt
     2161 tggaagcttc atgttataat taactggtta atgaaagagg agatttatgg ggactttaga
     2221 aaaaaaactt gataacttag taaatactat tttattaaaa gcagaaaatc agcatgagtt
     2281 attatttgga gcttgtcaaa gtgacgttaa gcttactaat acgcaagaac atattttaat
     2341 gttactatct cagcaacgtc tcactaatac agatttggct aaggcattaa atattagtca
     2401 ggcggcagta actaaggcta tcaagagttt ggtcaaacaa gacatgctag caggaactaa
     2461 ggatacggtt gatgctaggg tgacttattt tgaattaacc gagttagcta agcctattgc
     2521 atcagaacat acccatcatc atgatgaaac cttaaatgtt tacaaccgtt tattacaaaa
     2581 attctccgcg aaagaattag agattgtaga taagtttgta acagtttttg ctgaggaatt
     2641 agaagggtaa tccatgagat acatatcagt gaaaaatctc tcctttcaat atgaaagtga
     2701 gccagtttta gaagggatca cttatcattt agatagtgga gaatttgtca ccatgaccgg
     2761 tgaaaatggc gctgcaaagt caaccttaat aaaagcaact ttaggaattt tacaaccaaa
     2821 ggctggacga gttactattg ctaaaaaaaa taaagacggt aaacaattaa ggattgctta
     2881 cttgccgcag caagtagcta gctttaacgc tggttttcca tccaccgttt acgagtttgt
     2941 caaatcaggt cgctacccac gtagtggttg gtttagacgt ttgaacaaac acgatgaaga
     3001 gcatgtgcaa gcaagcttag aagcagtcgg catgtgggaa aaccgtcata agagaattgg
     3061 tagtttatca ggtggtcaaa aacaacgtgt ggttattgcc cgtatgtttg cttctgaccc
     3121 tgatattttt gtgctagacg agccaacaac gggaatggat agcggtacta ctgatacctt
     3181 ttatgaactg atgcaccaca gtgcacatca acatgggaaa tccgttctga tgattaccca
     3241 tgacccagaa gaagtgaagg cttatgctga tcggaacatt catttagtca gaaaccaaaa
     3301 acttccttgg cgttgtttca acattcatga agttgaaaca gatgacgaaa aaggaggtca
     3361 tggtcatgct tgatatttta tcctatgatt tcatgcaacg ggcggtaatg gcggtagttg
     3421 ccattagtat ttttgctccg attttaggta ttttccttat tttacgtcgt caaagtttga
     3481 tgagcgatac ccttagtcat gtttctttgg ctggggtagc gcttggggta gtcattggta
     3541 tttcaccaac catcactact attattgttg tggttttagc tgctattttg ttagaatacc
     3601 tgcgtgtagt ttacaaacac tacatggaga tttcaacggc gattttgatg tcacttggtt
     3661 tggccctatc tctgattatt atgagtaagt cgcatagttc atcaagcatg agtttagaac
     3721 aatacctttt tggatcgatc atcacgatta gtatggaaca agttgtcgcc ttgtttgcta
     3781 ttgctgcgat tattttaatc ttgaccgttc tcttcattag accgatgtac attttgacct
     3841 ttgatgaaga tactgctttt gtagatggtt tgcccgttcg cttgatgtct gttctattca
     3901 atatcgtcac tggggttgct attgctttga ccattccagc agcaggagca cttttggttt
     3961 ctaccattat ggtcttgcca gcaagtatcg caatgagatt gggtaaaaac tttaaaacag
     4021 ttatcttact gggaattgtc atcggtttta gcggtatgtt atctggtatt ttcttatctt
     4081 atttctttga aacaccagct agtgccacta ttaccatgat tttcattagt attttcctct
     4141 tagttagtct aggtggaatg cttaaaaaac ggttatttta ataagaaaaa tcctcacatg
     4201 tttaacatgt gaggatttat ttataaatgc tttaaaaagg gggcaaggtc ccaattttct
     4261 tgattaatgg tgatgccctc ccaaaagcta tcattagtta aacgaggaat caaatgaagg
     4321 tgaaaatgag tcccctcatc catcaactcc ttgtcattgc tgacgctagt gacgcctgaa
     4381 atgggaaggt gctgctctag cttagcaact agagcggctt gtaagtcgat gagatcatag
     4441 cgcacttcct ttggtagttg tgttagacta gtgtaatggt ctttggaaat cagtaaaagg
     4501 tgcccctttt ggataggatc aatatcaaaa acaaccttga aatattgact ttgagtcagt
     4561 agctgatttg ctttgagctg atggcaaaag atacattctg tcataggtac atctccttat
     4621 gaaaaaacga gccagagctc gtttaatcgg ttatctagta agtgagaaca gcgtattttt
     4681 tcttaccgcg gcgaataacg gttaattgat catcaatttt atcatcatta cttaattggt
     4741 aatctaaatc ttgaacgcgg tctccgttaa tgtagattgc tccattttgc acgtcttcac
     4801 gcgcttggcg ttttgatggg gaaatacctg cggtgacaag catgtcaaca aggttaaggc
     4861 tgtcttctga ttgaacgtga taatttggta cattgctcaa tccttgtttt aattcattag
     4921 cagaaagatt tttgatgttt ccagcaaata actgctcggt aatgttaaga gcttgtttat
     4981 aaacttcttc gctatgaacc aaggtaacca cttcgcgtgc caaggttttt tgagcgagac
     5041 gctcgtgacg agcggcatta aattgagttt caatttctgc aatctcatct agagataaga
     5101 aggtgaagat tttcaagaaa cgcactgcat catcatccat gacatttaac cagaattggt
     5161 acatctcgta aggagacgtc ttatcggcat caagccagac agcattacct tctgatttac
     5221 cgaatttttt cccggttgag tcagtgatga gtggtacagt catgacgtgc ccagttttat
     5281 cagccttttt gcggagcaat tcagtaccag ctgtcatatt accccactgg tcagagccgc
     5341 caatttgtaa ggttacatta tgcttgtcat tgagttcgta gaagtcgtag ccctgcatga
     5401 tttggtaagc aaactcagtg taagaaatgc ctgtttcaat gcgtttttta acagagtctt
     5461 tactcatcat gtagttaacg gtgaagtatt taccgacatc acggaggaag tcaataaagc
     5521 tgatttgcga gaaccagtcg tagttgttga caagctctgc tttattatca ccattttcaa
     5581 aatcaaggaa agtagacaac tgccctttaa tcttgtcgct ccactccaaa actgtttctt
     5641 tggtttgaag gctgcgctct gcatctttaa aggaaggatc accaattaaa cctgtggcac
     5701 cacccacaag agcgtaaggt ttgtgccctg ctagttgcaa gcgacgagat gttaagatag
     5761 ccactagatg gcccagatga aggctgtcag cggttggatc ataaccggta taataggata
     5821 cttgcccttc tgttaatgct ttgacaaggg cttgttcatc agtcgtttga aagaccaagc
     5881 cacgagcttt gagttcttca aaaatattca taggctttct ccattttcat tatagtttcg
     5941 aatcattata tcaaaaaacg ctactgctat gcaagcttta tcaaagtttg gtataatgat
     6001 aaatgaggta ttttatggtg aaatggaaca cgaaacaaaa gcgtataagt catcaaagat
     6061 taggtctctt ggatttgggg ccggtgctat tacgtacgtt gagactactg tctaactttt
     6121 tttatattgt tatcttcctt tttggaatga tgggatttgg tatggcattt gggtatttgg
     6181 ctagtcagat tgaatctgtt aaggtaccaa gtaaagaaag tttagtcaaa caagttgaat
     6241 cattaacgat gatttcgcaa atgaactatt ctgataatag tttaatttct actttagata
     6301 cggatttact tcgaacacca gtagctaatg atgcgatttc agagaatatc aaaaaagcta
     6361 ttgtatcaac agaagacgaa cattttcaaa aacataaagg tgtcgtgcca aaagctgttt
     6421 ttcgggcaac attggcttct gtattgggat ttggagaagc tagtggaggt tcgaccttaa
     6481 cacagcaatt ggtcaaacaa caagttttgg gagacgatcc cacatttaag cgcaagtcta
     6541 aggagatcgt ttacgctctt gccttagagc gttatatgtc caaagacaat atcttatgtg
     6601 attatcttaa tgtttcacct tttggtcgta ataacaaggg ccaaaatatt gctggtgttg
     6661 aagaagctgc gcgtggcatt tttggcgttt ctgccaaaga tttaacggtg ccacaggcag
     6721 catttttggc ggggcttccg cagagcccta ttgtttactc tccttatttg tcaacgggac
     6781 aactgaagtc agaaaaggac atggcttatg gcatcaagcg tcagcaaaat gttctctata
     6841 acatgtaccg tacaggtgtt ctgtctaaaa aagaatacga ggactataag gcttatccga
     6901 ttcaaaagga ttttattcaa ccgggaagcg caatagtaaa taatcacgat tacctttatt
     6961 acacggtgtt agcggatgct aaaaaagcca tgtatagcta tttgattaag cgagataagg
     7021 tgtctagtcg tgacttgaaa aatgacgaga ctaaggctgc ttatgaagag agagccttaa
     7081 cagaattaca acagggtggc tataccatca ccacaaccat taataagcct atttacaatg
     7141 cgatgcagac agcggcagct cagtttggtg gcctgttaga tgatggcact ggcacagttc
     7201 aaatgggaaa tgtcttgaca gacaatgcga ctggtgctgt gttaggtttt gttggtggta
     7261 gagattatgc tctgaatcaa aataatcatg ctttcaatac agttagatcg ccaggttcta
     7321 gcattaaacc gataatcgct tatggtcctg ctattgatca aggtttaatg gggagtgcta
     7381 gcgttttgtc taattaccca acaacttact cgagtggcca aaaaatcatg catgctgata
     7441 gtgaaggaac agccatgatg ccacttcaag aggccctaaa tacttcttgg aacatcccag
     7501 ctttttggac acagaaatta ctgcgtgaaa aaggggtcga tgtcgaaaat tatatgacaa
     7561 aaatgggtta taagattgca gactattcga ttgaaagttt acctctaggg ggcggtattg
     7621 aaatctcggt tgctcaacaa accaatgctt accaaatgct ttcaaacaat ggcttatatc
     7681 aaaagcaata tattgtagat aagattactg ctagcgatgg tacagtcgtt tacaaacatg
     7741 aaaataagcc aattcgtatt ttttctgcag caacagctac gattttacaa gaattgttga
     7801 gaggtccgat tacttcaggc gctacgacta ctttcaagaa ccgtttggcg gctattaatc
     7861 cgtggcttgc taatgctgat tggattggta agaccggaac aactgagaat tatacggatg
     7921 tttggctagt cctgtctact ccaaaagtta ccttaggcgg ttgggcagga catgatgaca
     7981 atacctcatt agcgccatta acaggatata acaataattc taattatctt gcctatttag
     8041 ctaatgccat taatcaggcc gatcccaatg ttattggagt agggcaacgc ttcaacttag
     8101 atccaggagt cattaaggcg aatgtcttga agtcaacagg tttacaacca ggaactgtta
     8161 atgtcaatgg acatactttt tctgttggcg gagaaatgac caccagtcta tggtcccaaa
     8221 aaggaccggg ggctatgact taccgatttg ctattggtgg cacggatgcc gattatcaaa
     8281 aagcctgggg gaacttcggg ttcagaaaaa attaggttgc catttatcga taaaaagggt
     8341 ataatagtaa taactatttg tcgtccaatc tagctgaaat attgtccagt taggaagaac
     8401 agcagttaaa tcacactgat aaagtcagat ttagctgctc tttttgtgtc tatttttaga
     8461 aaagataggg tttgtaacct atatttaaat attctaaaaa ttaacattta gggcaaatga
     8521 taacttagtt gcgatttgct gaaacagcaa agcttaaata gaagaaggag ctaaaaactt
     8581 ggcaggacat gaagttcgat acggaaaaca ccgtacacgt cgtagctttt caagaatcaa
     8641 agaagttctt gatttaccaa atttgattga aattcaaact gactcattcc aagatttcct
     8701 cgattcaggt ttgaaagaag tatttgaaga tgtacttcct atttcaaact ttacggatac
     8761 tatggaactt gaatttgttg gttacgaatt taaagaacct aaatataccc ttgaagaagc
     8821 tcgtatccac gatgcaagtt attctgcacc aatctttgtg accttccgct tggtcaataa
     8881 agaaactggt gaaatcaaga cacaagaagt tttcttcggt gacttcccta tcatgacgga
     8941 aatgggtact tttatcatca atggtggtga acgtatcatc gtttctcagt tggtgcgttc
     9001 tccaggtgtt tatttcaacg ataaagttga taaaaacggt aaagttggtt atggatcaac
     9061 agtgattcct aaccgtgggg cttggttgga attagagact gactcaaaag acattgccta
     9121 cactcgtatt gaccgtaccc gtaagattcc attcacaacc ttggttcgtg cccttggttt
     9181 ctcaggtgat gatgaaattg ttgatatctt tggtgaaagc gaccttgttc gtaataccat
     9241 tgaaaaagac attcacaaaa atccaagtga ttctcgtaca gacgaagccc ttaaagaaat
     9301 ttacgagcgt cttcgtccag gtgagccaaa aactgcagat agctctcgta gtcttttgat
     9361 tgcgcgtttc tttgatgcac gtcgttacga tttggctgcg gttggtcgtt acaaagtgaa
     9421 caaaaaactt aatatcaaga ctcgtctttt gaaccaaatc attgctgaaa accttgtgga
     9481 tgctgaaact ggcgaaattt tggtagaagc tggaactgag atgactcgta gcgtcatcga
     9541 atccatcgaa gaacaccttg acggtgattt gaacaagttt gtttacacac caaatgatta
     9601 cgctgtggtt actgaacctg ttgttcttca aaagttcaag gttgtgtcac caattgatcc
     9661 tgatcgtgta gtaactattg ttggtaatgc caatccagat gataaggttc gtgcattgac
     9721 acctgctgat attttggcag aaatgtcata cttcttgaac cttgctgaag gtcttggaaa
     9781 agttgacgat attgaccact taggtaaccg tcgtatccgt gccgttggtg aattgcttgc
     9841 caaccaattc cgcatcggtc ttgctcgtat ggagcgtaac gttcgtgagc gtatgtctgt
     9901 tcaagacaac gatgtgttaa caccacaaca aatcatcaat atccgtcctg tcacagcagc
     9961 tgtcaaagaa ttcttcggtt cgtctcagtt gtcacagttc atggaccaac acaacccatt
    10021 gtcagagttg tctcacaaac gtcgtttatc tgccttagga cctggtggtt tgacacgtga
    10081 ccgtgctggt tatgaggttc gtgacgtgca ttacacgcac tatggccgta tgtgtccgat
    10141 tgaaacacct gaaggaccaa atattggttt gattaacaac ttgtcttcat ttggacatct
    10201 taataaatat ggtttcattc aaacacctta ccgtaaggtt gaccgtgcga ctggtagggt
    10261 aactaacgag attgtttggt tgactgccga cgaagaagac gaatacacag ttgcacaggc
    10321 caattcgaaa ctaaacgaag atggcacttt tgctgaagaa atcgttatgg gtcgtcacca
    10381 aggtaataac caagaatttt ctgcaagtgt tgttgatttc gttgacgttt cccctaaaca
    10441 ggtagttgct gttgcgacgg catgtattcc tttcttggaa aatgatgact ccaaccgtgc
    10501 tctcatgggt gccaacatgc aacgtcaggc tgtgccattg attgatccaa aagcaccata
    10561 tgttggtact ggtatggaat atcaagctgc ccatgactca ggcgctgcgg tgattgctca
    10621 gcacaatggt aaagttgtct tttctgatgc tgaaaaagtg gaaatccgtc gtcaagatgg
    10681 ctcacttgat gtttaccaca ttaccaaatt ccgtcgttca aactcaggaa cagcctataa
    10741 ccaacgcacc cttgttaaag taggagacat cgttgaaaaa ggtgatttca tcgctgatgg
    10801 gccttctatg gaaaatggtg aaatggccct tggacaaaac ccagtcgttg cttacatgac
    10861 ttgggaaggt tacaacttcg aggatgctgt tatcatgagt gagcgccttg tgaaagaaga
    10921 tgtctacaca tctgttcact tggaagaatt cgaatctgaa acgcgtgata caaaacttgg
    10981 ccctgaagaa atcactcggg aaatcccaaa tgttggtgaa gaagccctca aagaccttga
    11041 cgaaatggga attatccgta tcggtgctga ggttaaagaa ggcgacatct tagtaggtaa
    11101 agtcacacct aaaggtgaaa aagacctttc tgctgaagaa cgtttacttc acgccatctt
    11161 tggggataaa tcgcgtgaag tgcgtgatac ctcacttcgt gtccctcatg gtggtgatgg
    11221 tattgttcgt gatgtgaaaa tctttacacg cgctaacggc gatgaattgc aatcaggtgt
    11281 taatatgctt gtgcgtgttt acatcgctca aaaacgtaaa atcaaggtcg gagataaaat
    11341 ggccggtcgt cacggaaaca agggtgtcgt ttcacgtatt gtaccagttg aagacatgcc
    11401 ataccttcca gacggaacac cagttgacat catgttgaac cctctcgggg tgccatcacg
    11461 gatgaatatt ggtcaggtta tggaacttca ccttggtatg gctgctcgta atcttggcat
    11521 tcacattgca acacctgtct ttgacggggc ttcatcagaa gacctttggg acactgttcg
    11581 tgaagctggc atggatagcg atgctaagac ggtcctttat gatggtcgca ccggtgaacc
    11641 atttgacaac cgtgtatccg ttggtgtcat gtatatgatc aaacttcacc acatggttga
    11701 tgataaactt catgcccgtt ctgtaggacc atactcactt gttacccaac agccacttgg
    11761 tggtaaagct caatttggtg gacaacgttt tggtgagatg gaggtttggg cccttgaagc
    11821 ttatggtgca tcaaatgttc ttcaagaaat cttgacctac aagtcagatg acgtcacagg
    11881 acgtttgaaa gcctatgaag cgattactaa aggtaaacca attccaaaac caggtgtacc
    11941 agaatccttc cgtgttcttg taaaagaatt gcaatcgctt ggtcttgata tgcgtgtgct
    12001 tgacgaggat gataatgaag tggaacttcg tgatcttgat gaaggtgaag acgatgatat
    12061 tatgcatgtt gacgatctcg agaaggcacg tgaaaaacaa gctcaagaaa cccaagaagt
    12121 ttctgaaaca actgacgaaa aataagcaat caattcttat taaataatta tttactggtc
    12181 tggggcaaag gccccaggaa ctggtaaagt catcaaaggc agaaaggtaa aactagtggt
    12241 tgacgtaaat cgttttaaaa gtatgcaaat cacattagcc tcaccaagta aggtccgttc
    12301 atggtcttat ggtgaagtta aaaaacctga aacaatcaac taccgtacat taaaaccaga
    12361 acgtgaagga ctctttgacg aagtcatctt tggtccaaca aaagactggg aatgtgcgtg
    12421 tggtaagtac aaacgtatcc gttataaagg gatcgtttgt gaccgctgtg gggttgaggt
    12481 aactcgtgct aaagtacgtc gtgaacgtat gggtcacatt gagttaaaag ctcctgtatc
    12541 acatatctgg tatttcaaag ggattccatc tcgtatggga ttgactcttg atatgagtcc
    12601 tcgtgccctt gaagaagtga tctattttgc ggcttatgtg gtcattgatc caaaagatac
    12661 accgcttgaa ccaaaatcat tattaacaga acgtgaatac cgtgaaaaac tacaagagta
    12721 tggtcatggg tcatttgtcg ctaagatggg tgctgaagcc attcaagatc tcttgaaacg
    12781 tgtggacttg gcagctgaaa tcgctgaatt aaaagaagag ttgaaatctg cttctggtca
    12841 aaaacggatt aaagcagttc gtcgtttaga cgtccttgat gcctttaaca aatctggaaa
    12901 taaaccagaa tggatggttc ttaacatcct gccggttatt ccaccagatc ttcgtccgat
    12961 ggttcaattg gatggtggtc gttttgcggc atcagacttg aatgacttgt accgtcgtgt
    13021 gattaaccgt aataaccgtt tggcacgttt gttagaactt aatgcccctg gcatcattgt
    13081 tcaaaatgaa aaacgaatgc ttcaagaagc cgttgatgct ttgattgata atggtcgtcg
    13141 tggtcgtcca atcactggac caggaagtcg tccattgaaa tcattgagcc acatgcttaa
    13201 aggtaaacaa gggcgtttcc gtcaaaactt gcttggtaaa cgtgtagact tctctggacg
    13261 ttccgttatc gctgttggtc caacccttaa aatgtatcaa tgtggtgtcc cacgtgaaat
    13321 ggctatcgag ctttttaaac catttgtaat gcgcgaaatt gttgccaaag aatatgctgg
    13381 taacgttaaa gccgctaaac gtatggttga acgtggcgac gaacgcatct gggatatctt
    13441 agaagaagtt atcaaagaac acccagtctt acttaaccgc gcaccgactc ttcacagact
    13501 tggtattcag gcttttgaac ccgttcttat tgacggtaag gcacttcgtc ttcacccact
    13561 tgtgtgtgag gcctacaatg ccgacttcga tggagaccaa atggccattc acgtgccact
    13621 ttcagaagaa gcacaagctg aagctcgtct tttgatgctt gctgcagagc acatcttgaa
    13681 ccctaaagat ggtaaaccag ttgttactcc atctcaggat atggttcttg gtaactacta
    13741 tcttacgatg gaagatgctg gtcgcgaagg cgaaggtatg attttcaagg ataaagacga
    13801 agctgtgatg gcatatcgta atggttatgc tcatcttcat agtcgtgtgg gtatcgctgt
    13861 tgacagcatg ccaaacaaac cttggaaaga caatcaaaga cataaaatca tggtgacaac
    13921 tgttggtaag attttgttta acgatatcat gccagaggac cttccttacc tccaagagcc
    13981 aaacaatgcc aacttgacag aaggaacagc tgataaatac ttccttgaac ctggtcaaga
    14041 tatccaagaa gtgattgacg gcttagaaat caatgtgcca ttcaagaaga aaaacctcgg
    14101 taacatcatt gcggaaacct tcaaacgttt ccgtacaaca gaaacatcag ccttccttga
    14161 ccgtttgaaa gaccttggtt actaccattc aacccttgct ggtttgacag tgggtatcgc
    14221 tgacattcct gttattgata ataaagctga aatcattgat gctgctcacc atcgtgttga
    14281 agaaattaac aaagccttcc gtcgtggttt gatgacagat gatgaccgtt atgttgccgt
    14341 tacaacaaca tggcgtgaag ctaaagaagc ccttgaaaaa cgtctgattg aaacacaaga
    14401 tcctaagaac ccaatcgtta tgatgatgga ctcaggagct cgtggtaaca tctcaaactt
    14461 ctcacagctt gctggtatgc gtggtttgat ggctgctcct aacggacgca tcatggaact
    14521 tcctatcttg tcaaacttcc gtgaaggttt gagcgttttg gaaatgttct tctcaaccca
    14581 cggtgcacgt aaagggatga ccgatacggc ccttaaaaca gccgactcag gttaccttac
    14641 tcgtcgtttg gttgacgttg cccaagatgt tatcattcgt gaggacgatt gtggcactga
    14701 tcgtggtctt cttatccgtg ctattacaga tggtaaagaa gttaccgaaa cgcttgaaga
    14761 gcgtcttcaa ggtcgttaca cacgtaaatc agtcaaacac cctgaaactg gtgaagtctt
    14821 gattggtgct gaccaattaa tcactgaaga catggctcgt aagattgttg atgcaggtgt
    14881 tgaagaagtg accattcgtt ctgtctttac ctgtgcgact cgtcatggtg tctgccgtca
    14941 ctgttatggt atcaacttgg caactggtga tgctgttgaa gtgggtgaag cagttggtac
    15001 tattgctgcc caatctatcg gtgagcctgg tactcagctt accatgcgta ccttccacac
    15061 gggtggtgta gcctcaaata ccgatatcac ccagggtctt cctcgtattc aagagatctt
    15121 tgaagcacgt aatcctaaag gggaagcggt cattactgaa gtgaaaggga atgtcgtcga
    15181 gattgaagaa gatgcgtcaa ctcgtaccaa gaaagtctac gttcaaggta aaactggcat
    15241 gggcgaatat gtcgtaccat ttacagcacg tatgaaagtt gaagttggcg acgaagttaa
    15301 tcgcggagct gcccttacag aagggtcaat ccaaccgaaa cgtctccttg aagtgcgtga
    15361 taccttgtca gttgaaacgt accttcttgc agaagtacaa aaagtttacc gtagccaagg
    15421 ggtagaaatc ggagacaaac acgttgaggt aatggttcgc caaatgcttc gtaaagttcg
    15481 tgtcatggat ccaggtgata cagacctcct tccaggtaca cttatggata tttctgattt
    15541 cacagatgct aacaaagata ttgttatctc tggtggtatt cctgcgacat ctcgtcctgt
    15601 tcttatgggt attactaagg cttcccttga aaccaattcc ttcttatcag ctgcatcctt
    15661 ccaagaaaca actcgtgttc ttacagatgc tgctatccgt ggtaagaaag accacttact
    15721 tggtcttaaa gaaaatgtta tcattggtaa aatcattcca gctggtactg gtatggcgcg
    15781 ctaccgtaac attgaaccac aagcgatgaa tgagattgaa gtgattgatc atacagaagt
    15841 ctcagcagaa gccgttttta cagcagaagc agaataatct catgaaagag tccttagggg
    15901 ctcttttttg tctgagtttg ttggcagtaa aaagcattga caatcagtct tagtcccaat
    15961 gagcagatgt ttttcaaaat acttatgatt tagctataat aatgcataag aggaataggt
    16021 aatgtgttat gtatcaagta attaaaatgt atggtgattg ggagccttgg tggttcatcg
    16081 atggatggca agacgatatt atagatgagc aacaatttag tgactggcaa gaagcgcttg
    16141 attattttaa tcaagaatgg cagcgcatga aagctatttt tcctagttat catagtcaaa
    16201 agaatttgtt agctactttt tgggaaaaag aagataaaag atggtgcgag gactgtgacg
    16261 aagatttaca gcagtttcat tctcttttac tccttaaaaa taaagatatt gtaccaagca
    16321 ataattatat ccctgaattt gaacaacgaa atgattcacc acaggtagct tatctttgca
    16381 agttaaacct ttaagtgttt gttaaaaaca aaataagtta aaagaaaata tcacttcttc
    16441 tgatattttc tttttttgtt tacgaataaa tgagtaagga ggaattatgg tacaagcatt
    16501 agcaaaagct attctagcaa aagctgaaca ggttcaggca caagatattt atattttgcc
    16561 aagagcagat caatatgatc tttttttacg aataggagat gaaaggagat tagttgatgt
    16621 ttatcagagc gatcggatgg ctcctcttat tagtcacttt aaattcgttg caggaatgat
    16681 agttggtgaa aagagacgtt gtcaggtggg ttcatgtgat tataagctta gtaaagataa
    16741 gcagttatct ttgcgcttat ctagcgtggg tgattatcgc gggcatgaaa gcttagtgat
    16801 tcgtctgctt catcatcaaa ataaaagtgt acattattgg tttgatggat tgacaaaagt
    16861 agccaatcaa gttggcggta gagggttgta tttatttgca ggaccagttg ggtctgggaa
    16921 gacaaccttg atgtaccagc tgatttcgag ttatcatcaa gaagcacagg ttattagtat
    16981 agaagatcct gtagaaatta aaaatcacca gattttacaa ttacaagtga atgatgatat
    17041 tggtatgact tatgacaatt tgatcaaact gtctttacgc catcgaccag atattttagt
    17101 tattggtgag attcgagata gtcaaacagc aagagccgtt attagggcta gtctaacagg
    17161 tgccatggtt ttttcaacgg ttcacgctaa aagtatctcg ggtgtttatg caagattgtt
    17221 agaacttggt gtaacgaaag cagaactgtc taattgctta gcattaattg cttaccaaag
    17281 gttacttaat ggaggagcat tgattgactc tactcaaaac gaatttgaat attattcctc
    17341 atcgaactgg aatcaacaaa ttgatcagct tcttgaggca ggacatctca atcccaagca
    17401 agctaagctt gaaaaaatta tctagtaaac atcagcataa attcattcaa ttactagcta
    17461 atcttttatc tacaggattt agcttcacag aggttattgc ttttttaaaa agaagccagt
    17521 tgcttcagct agattatgtt cttaaaatgg aagagtcttt attaaaagga caaggcttag
    17581 cagatatgtt gtcgggttta ggtttttcag atgctattct tactcaaatt agtttagctg
    17641 atagacacgg taatattgaa acaacattag tagcgattca acattacttg aaccaaatgg
    17701 caaggatcag acgaaaaact gttgaagtta tcacctatcc tcttattctt ttactttttc
    17761 tttttgtaat gatgctaggg ttgagacgct atttagtccc tcaattagaa actcaaaatc
    17821 agataactta ctttcttaac cattttcctg ccttctttat aggtttttgc tcaggtctta
    17881 ttttgttgtt cgggatggta tggctacgat ggcgatctca aagccgcttg aaactctata
    17941 gtcggctaag ccgttatcct tttttaggta aactgttaaa acaatactta actagttatt
    18001 atgctagaga atggggtacg ttaataggtc aaggtcttga tttgatggct attttagaca
    18061 ttatggccat tgagaaatcc tcactaatga aagaattagc tgaggacatc agaatgagtc
    18121 tgcttgaagg gcaagccttt cacattaaag tagctaccta cccttttttt aaaaaagaat
    18181 taagtttaat gattgaatac ggtgaaatca aatcgaaact gggggctgag ttggaaatct
    18241 atgcccagga aagctgggaa caatttttta gtcagcttta tcaagtgaca caacttattc
    18301 aaccagctat ttttttagta gttgctgtga caatcgtcat gatttatgcg gcaatcctat
    18361 taccaattta ccaaaatatg ggaggtattt tttaagatga ttaatcaatg gaacaactta
    18421 cgacacaaga agctaaaagg atttactctt ctagaaatgt tattggtgat tcttgtcatc
    18481 agtgttttga tgctattatt tgtgcctaat ttaagcaagc aaaaagacag ggttacagaa
    18541 acaggtaatg ccgctgttgt taaattagtg gagaatcaag cagaactata tgaattatct
    18601 caaggctcaa aaccaagttt gagccagtta aaggcagatg gtagtatcac tgagaaacaa
    18661 gaaaaagctt atcaagacta ttatgacaaa cataaaaatg aaaaagcccg tcttagcaat
    18721 taaagctttt actttgcttg aaactttact aagtttgtca gtgatgagtt tcattatatt
    18781 gggattgtct gtaccagtga caaaaagcta tcaaaaagta gaagagcatc tgttttttag
    18841 ccattttgaa catttgtatc gacatcagca aaagttagcc atattacagc aaaaacagcg
    18901 cgtcttggat atttcttcaa caaaaattgt tacagaaggt aatagcctaa cagttcctaa
    18961 atcaataaca gtgaatcatc cttatcggtt agttattgat cagatgggag gcaaccattc
    19021 cttagctaaa attatttttg acatgactga tcgacgcttt aaataccaat tttatctagg
    19081 gagtgggaat tatcaaaaaa caagtcaaag cttacatagc ccttgaaagt atgattgcaa
    19141 caggcatcct ctttagtatt gtcattctcg tcttaagcag tttacagcag agtcaggctg
    19201 ctctaacgta ctatcgaaag cagcaagaaa agcttaatct agccttgatg gcagtacaaa
    19261 ctagaactaa agagataaca ttgaatggtt gtcatattac tattttacgt acggatagat
    19321 acattagtat tcacgatgac gaaggagaag tgatgaagat tgagtaaaca attaagtaac
    19381 ataaaagctt ttactcttct agaggcgtta atagccttac tcgtgatatc agggtcttta
    19441 ttggtttatc aaggtttgac ccaaaccctc cttaaacgta gccattatct agcccgtcat
    19501 gatcaagaca attggctctt attttctcat caattgcgag aggagttaag tggagcaaga
    19561 ttttacaaag tagctgataa taaactatac gttgaaaaag gaaagaaagt actagctttt
    19621 ggccaattta aaagtcatga tttccgaaaa tcagctagta atggaaaagg gtatcaaccc
    19681 atgttatttg gaatatcacg taatcatatt cacatagagc agtcacagat ttgcattact
    19741 ttaaagtgga aaagtgggtt agaaaggact ttttattatg cctttcaaga ctaatctcaa
    19801 agcaggcatc cttttgtatg ctatgtttat ggctagtatt tttttgctgg ttttacaggt
    19861 ttacctttct caagtgacag ctctacacaa ggaataccaa gctcaaactg actatgttaa
    19921 agcccgactg atagcagaaa tagtttatca agatcataga tataaagctt ctaatcctgt
    19981 ttcttttaaa ggtggtcaag tcatttgtcg agagcgaaaa gagagatgga tgttaattgt
    20041 taagttagat cagcaacgac agtatcagtt tgaatacctt aaacccaaac aatatagtca
    20101 ataaatagta ttgttgtcat aaatccttaa aatgatccaa tatatagtaa ttaaaggtga
    20161 cacttctttt catttcaagt gtaatttggt atgataaggt tatgactttt gaaaaaattg
    20221 aagaagctta tcagctgctc ttagagaact gtcaactaat tgaaaatgat ctaaaaacac
    20281 atatatacga tgccattgtt gaacaaaact ccttttattt aggggctgag ggagctagtc
    20341 ctcaagttgc tcaaaatagt gataaactga aagccttgtg cctgacaaaa gaagaatggc
    20401 gtaaggccta ccagtttctt tttattaagg cagctcagac tgagcaactc caagccaacc
    20461 atcagttcac accagatgct attggcttca ttctgctgta tcttttggaa caattgagtg
    20521 ataaagatag cttagaggta cttgagattg gaagtggaac agggaaccta gcccaaaccc
    20581 ttctcaacaa cacgagcaag agccttgatt atgtagggat tgaacttgat gatctcttga
    20641 ttgatctgtc agccagtatt gctgagataa tggattcttc agctcatttt attcaagaag
    20701 atgcggtaag gcctcaatta ctaaaagaaa gtgacattgt catcagtgac ttaccagttg
    20761 gttattatcc taacgatgat attgccaaac ggtacaaggt ggctagttca gataagcata
    20821 cctatgccca tcatttatta atggaacagt ctttaaaata cttgaaaaaa gacggttttg
    20881 cgatttttct ggcaccagtc aatttattga cgagccctca gagccagtta ttgaaacagt
    20941 ggttaaaaga ttatgctcag gtggtgacct tgattacgct accagattct atttttggtc
    21001 atccctcaaa tgccaagtcc attattgtct tacaaaaaca aacagaccac ccaatggaaa
    21061 cctttgtcta tccaattcgg gatttgaagc ttgcagagaa tattcatgat tttatggaaa
    21121 atttcaaaaa atggaaactg agtaatgtca attaattgat tttttgttat aataaaaaag
    21181 catcacagaa aacgatttca agagaggtta aaaatgtcaa agacaattgc aattaatgca
    21241 ggtagttcta gtttaaaatg gcaactttat cagatgccag aagaagcagt gttagcacaa
    21301 ggaattattg agcgtatcgg tctcaaggat tctatttcaa cagttaagta cgatggcaaa
    21361 aaagaagagc aaattcttga tatccacgat catacagaag ctgttaaaat cttattgaac
    21421 gatttgattc actttggaat cattgcagct tatgatgaaa ttacaggagt tggacaccgt
    21481 gttgttgctg gtggtgaact tttcaaagaa tccgttgtgg ttaatgacaa agttcttgaa
    21541 catattgaag aattatcagt tttagctcca cttcacaatc ctggagcggc ggcagggatt
    21601 cgtgccttcc gtgatatttt gccagatatt accagtgttt gtgtgtttga cacgtcattt
    21661 cacactagca tggcaaaaca tacttacctt tacccaattc ctcaaaaata ttatacggac
    21721 tacaaggttc gtaaatatgg ggcgcatggg acaagtcata aatatgtagc gcaagaagct
    21781 gctaaaatgc ttggtcgtcc attagaagaa ttaaaactaa ttactgctca tattggaaat
    21841 ggtgtctcaa ttacagctaa ttaccacggt aaatcagttg atacctcaat gggattcact
    21901 ccattagctg gtcctatgat gggaacacgt tctggtgaca ttgacccagc tatcattcct
    21961 tatttaattg agcaagaccc agagttaaaa gacgctgctg acgttgttaa tatgttaaac
    22021 aaaaaatcag gtcttagtgg ggtgtcaggc atttctagtg atatgcgtga tatcgaggct
    22081 ggtttacaag aagataatcc agatgctgtt ttagcttaca atatctttat tgatcgcatt
    22141 aaaaaatgca ttggtcaata ttttgctgtt ttgaatggag cagatgcttt ggtctttaca
    22201 gctggtatgg gtgaaaatgc accattgatg cgtcaagatg ttattggtgg cttaacatgg
    22261 ttcggaatgg acattgatcc tgagaaaaat gtttttggat accgtggtga catttcaact
    22321 cctgagtcaa aagtgaaggt gcttgttatc tcaacagacg aagaattgtg catcgcgcgt
    22381 gacgttgaac gtttaaaaaa tacaaaataa aagagactaa gtctcaatag attaaccaag
    22441 taaggtaaca ctttacttgg ttttttatga ggtttatctg ttggttatgg tgcctaggta
    22501 ttagttctga tcagtctcct tgctttttgt aatcttgtgc taggcgattt ggatgtcagc
    22561 cttatttaga agtttttaat tgcttcaacg cttttcttgt atgtgtttat agagcccaat
    22621 gatctaaaag actatttgat aacggtttat gatgagcgta atcgtgcgat tcgaaaacag
    22681 acacaagttt actgctttta cctgatgatt gttctatttg ttgccttatt ctttttcttc
    22741 ccttgcttaa ccatttgaga ccttgtttct tataagctac ctttgatttt tgcttacctt
    22801 tacttgatta tcagaagtct ggtggctaag aaaatggagg cactagggaa aataacaact
    22861 aatagtcaaa aacctccgtt tgatacgagt caggttcttt gttagatgac ttgtctaacg
    22921 gttgagtttc ctgtctggtc aacggaggtg tttgtattgt tgtctgattt gtattggggc
    22981 acttatttta caagctttta gccttatcga tggttttgtc aatggcagag ctgacagtag
    23041 ttgtgaggcc aaggcgttct aactccatta gaccagcaat agttgtgcca ccggggctac
    23101 aaatagcgtc aatgaaatcg tgcggacttt gagaactggt cttgagattg ctggcactag
    23161 ccaagactgt ttgagtaaca atctctagcg cctttgcttt aggcatgcca ttcttgacgc
    23221 cagccttagc caaagcctca ataaagagat aaatataggc aggacttgac cctgctaaag
    23281 cggtaaaggt gtcaaaatcc ttttcactaa tatcaaatgt gctaccaaag ctatctgtta
    23341 agtctcgaac acgtgcttgt aattcctggg acaccaaagc atttcccgtt aaagcggtac
    23401 tgctttggag aatttgtgca ttcatgtttg gcatgatacg tagcagcgga aggtcttgtc
    23461 ctacgaatgt tgctagtcgt tgaagggaaa tgcctgctgc catagatata ataggctgtt
    23521 tgaagtgaag cggtttgagt accgtttcaa atagttgagg cttgatgcct aaaataacaa
    23581 gatcaacctg gtcaataagg tcttggtggg acatagcata aggcagtgtt aactgctccg
    23641 caatttcctt ggaccgttct aaagatgatc ctgaaatgat aagttcatgg ggtgtttgtt
    23701 taaggccttt gatgatagcg ctagccattt tgccaacacc aataatgcca attttcattc
    23761 tgttgctcct tgtgggatat tacggttaat caggctaacg ctagtttagt aacgtttaat
    23821 caagtcaacg gttgagcgat ccagtttttt gataatggct tgtaagaagg cttgggcttc
    23881 tacgaaatca tccatagcgt agagggtttg atgagagtga atgtagcgtg cacagactcc
    23941 gatggttgtt gatgggacac caccattttg aaggtgtgca gcacctgcat ctgtgcctcc
    24001 cttgccacaa tagtattgga aattgacacc agcgtcctca gcagtagtca gtaagaagtc
    24061 gcgcatatct ttgagcatga catggcctgg gtcgtaaaag cgcaataagg taccatctcc
    24121 gattgttcca ggattgccat aaatatcacc agcaggcgaa caatctactg cgaaaaagag
    24181 ttcagggtcg aacttggtgg ttgagacgtg ggctccgcgc agaccaactt cttcttgaac
    24241 gttagcacct gcaattaggg tgttgttaag gtcttgtcct tttaacgctt caagcatttc
    24301 tgttatcatg agaacgccat agcgattgtc ccaagctttt gaaataatat ttttttgatt
    24361 ggctgttagg atcgtttcag attgagggac aataatatca cccggtgtaa tgccaaagct
    24421 ttcagcttct gccttatccg taaagccacc atcaaacaca atatcttcga tatttggtag
    24481 actagcagag ccatttgccc cacgtaaaaa atggggaggt accgatcctg aaataagggg
    24541 aataacttgg ccagtgcgtg tgtaaagggt aaaccgttgt gaactgacaa caagtgggtt
    24601 ccaaccaccg ataccaacca cgcgtagcgt tccgtcaact ttgatatcac tgaccataaa
    24661 accgacttcg tccatgtgcg cagctactaa aatacggggg gctttttcag ctttactatc
    24721 tctgatacca aaaatgccac caagcccgtc tgtttcaaca cggtcaacca gcggggttat
    24781 tttggtgcgt aggtagtcac gaacgctatg ttcatagccc gcaatgccat ccagttcggt
    24841 aacttctttg atttttgaga ataagtctgt catttttcat ctccatttaa aaggtttcag
    24901 accagttcaa cgcggtcatc tccttatatt ctaccacttt ttaagtaaga atgggtttca
    24961 cagaaaagaa aaatcccaga gactagagat ctctggaaga agtgtttgtc ttttggcgat
    25021 aacctgtttt tttaggcagc aaggaaatca ttaaaatgat aaagcacaag ctggttaata
    25081 agaatagtag ggcgcttagg atgcgaaaag actcttcact agcgaaaagt aaggataatg
    25141 gagtcataag gagaaccttc cattttaggt taatcagtga tttttatagc gtggttaaat
    25201 taataataaa ccacacctaa tcaacgtacc tttagtttag ttgtttttac tatttttgtc
    25261 aatacataac agtagaaatc tttagttttc ttatttcaga tcgtccaatt tgatcttttt
    25321 atgataaaat gagggatatg agtaaaaaga aaataggtat gatttcaggt atctttggat
    25381 ttagtttagc tattggccta ggaatagtta tcaaagatta ttgccaagac aggcagcgtc
    25441 gacaaatgac aagggattta cgcacctttt tttcaccttt aggacaaatc gaagttttat
    25501 atatcaatcc ttgtcaggtc aaacaagatt atatttcagg tggggttgtt atgtcaaacg
    25561 gcaaacagta ccaattcacc tatcacagtc gacaaattag ttttgaggag gacaagggat
    25621 gatcagaccg acttcttatg agtcgttagc ggctcttatt gaaaaagaag ataagctggt
    25681 gttatttttt acggcagact ggtgtccaga ctgtcaattt atttatccta ttatgccaga
    25741 aattgaagca gaacttacag atatgacttt tgtttgtgtt aatcgtgatc agtttataga
    25801 ggtagcacaa aagtggaata tttttggtat tcctagtttt gtcgtgattg aaaaaggtca
    25861 ggaagtaggg cgtctggtta acaaaatgag aaagacaaaa actgagatta tgcatttttt
    25921 agctgcttat caataagaaa agaggaagaa taaatgattt ttgcatacaa taaagaacaa
    25981 gttggcgatg tcttgatggt tatcttacaa gacaccaaag atatcaaacg tcaagtagaa
    26041 cgaaaaggca aagtagcccg tgtttttgca gaagaaagcg gcaaaaccct tgcttggaat
    26101 atctttgaag cgtcaagctt gattactatt gaaggcaatg gacagatttt tttgacagac
    26161 gaggaccttg caagattaaa tgcagagctt gctaaggaag gattttcaga aaggcttgaa
    26221 ccgattgtgg gacctgtttt tgtggttggt caaattgttg aaatggtggc tcatccagat
    26281 agcgaccatc ttaatatctg tcaagtggct attggtgaag atcaaacggt tcaaatcgta
    26341 gcgggcgcac caaatgctgc gcttggttta aaaacgattg ttgccttacc aggtgctata
    26401 atgccaaatg gtagtctgat tttcccagga aaattgcgtg gtgaagaaag ttatggtatg
    26461 atgtgctctc ctcgtgagtt agccttgcca aatgcaccgc aaaaacgtgg tattattgaa
    26521 cttgatgagt cagctgtggt aggagaagct tttgacccag ccaaacattg gaaaggttag
    26581 aaaaaccagc tgaaataaga ctgtatttaa ctaaatgacc agaaaaaaag gattaggagc
    26641 ccttaatcct tttttcttat gttttcaaaa acgtttaaaa tgaaacttat tttaccacaa
    26701 tttttacgaa taataagata aggaggtcag tatgtataat aaagtgatag caatcggtcg
    26761 tttggtagct aaaccagaat tggtaaaaac agctacggat aagcatgtag cacgtctctc
    26821 tttagctgtt aatcgaagat ttaaaaatgc ttctggagag cgagaagctg attttatttc
    26881 agttgttgtt tggggaaagt tagcagaaac tctggtttct tatgctagca aaggtagttt
    26941 gatgtctatt gatggcgaac ttaggacccg caagtatgat aaagatgggc aagtgcatta
    27001 tgtgacagaa gttctctgcc aatcatttca actgcttgaa agtcgtgctc agcgcgctat
    27061 gagagaaaat aatgttacta atgatctagt tgatttagtc ttagaagaag atactcttcc
    27121 cttttgatag aggctaatgc ttctcatcag ggttcagctg attactgaaa tatatgatga
    27181 ggtgatatcc gagcaacaaa agtgtgacta gatacttctg ataaaaataa aaaaacacct
    27241 tacgagtatg aacaaagttt caagtgctag atgttggaac tttgttcata ctcgtaaggt
    27301 gttttttagt gtattacttc tttttgttgc tagtgttaag gtttacttgc ttacaagcgg
    27361 ttatcaggcc actagaactc ttataataaa tggagctctt tgagtttggt gtcaatcatt
    27421 tttaagacag tcgccaaatc ttgtgggttc tgaacaaaat caagggaatt gccatcaatt
    27481 ttcatttttg gagagacctc gtagtcttcg taccaggttg ggtattcacc gtggacttga
    27541 tggtaatatt gctcaagact aggattgccg tcgacctgct caaaacttcg gccacgtttt
    27601 tcaatgcgct caagcatttt gtcaaaagac acatcaatgt agatgagaag atcagggcgt
    27661 tttttaggca tgccttctag ttcttctaac atgttggcga gaagttcctg atagatgtca
    27721 agttctgtct tagtgacatt gccatttttg tagttaagtt ttaagaagag ttcgtcttca
    27781 aaaatagagc ggtcaagaat gttgttatcg gcttgataag cttctttgat ggacttaaac
    27841 cgtttattca aaaaataaat ttgaagtaaa aaggcgtact ttttagggtc ttggtagtag
    27901 aggtctagca cggggttatt atcaacagct tcgtaaaaaa cgtcagttcc taaatgctct
    27961 ccaagtgcag cagcaagcga gctcttgcca gccccaattg ttccagctag tacaatcaac
    28021 atgcgtctcc ttctaatgtc attagcgtac tgtttcgaag atggcttcaa cctcagctag
    28081 agtctcagcg cgtgaaacag caccacgaac tttggcagca cctgctgttc cacgaagata
    28141 gtgtggcgct aagccccgaa attcccgaac agcaatggtt tcacctttga ggttaattag
    28201 gcgtttgagg tggtctttgg cgatgtctaa ttttttggca aagggaagat ctggcaattc
    28261 ttgaccagtt tcaaagaaat gattgatttg ggtaaaaaga taaggattgt tcatggctgc
    28321 gcggccaatc atcacggcat ctacaccgat ttcttcaatc ataaatttgg cgtcttgcac
    28381 cgaacgcacg tccccatttc caataaaggg aatcttagtg atagccttag aaactcgggc
    28441 taatgtctca tggtcgcagg ttcctgtata catttgctca cgggtacgac cgtgcatggc
    28501 aagagcagat acacctgccg actcagcggc aagggcattt tcaacagcaa gtgaactatc
    28561 cgcccaacct gtacgcattt tgacagtcag tggaatatct aaaacagacg tgacttcctt
    28621 gacaatgtgg taaatcttat cagggtcacg gagccacttg gcaccagcct cgtttttgac
    28681 aactttatta acagggcagc ccatattaat atcaacaata tcggccttgg tattggtttg
    28741 aatgaaatca gctgcacgtt tcaacccttc tgcatcacca ccaaataatt gaatggacat
    28801 agggtgctca ttctcgtcaa tatggagcat atggagggtc ttttcgttgt tgtagagcag
    28861 ccctttttcg gaaatcattt ccataaccac caaaccagca ccaaattctt tggcgatggt
    28921 acgaaaggca gaattggtca caccagccat gggagcaagg acagtgcggc gagggatttc
    28981 cacatcacca atcctaaagg aagaattaag ctttatcatt gatgatggcc tccaaatcac
    29041 tttcattaaa ttggtacttg gtaccacaaa attggcaaac aatctcagca cccttatctt
    29101 cgtcaatcat ggcctgtaaa tctgcttttg gcaaagtcat tagggctgct tcaaaacgtt
    29161 ctctagaaca gtcacactga aaactcagag gttcttcaga aagacgcttg taaggttcat
    29221 ctccatagat ggcctccaaa agggcatcca catgattttt ggaagctagt aggtaagaaa
    29281 tggcaggcat ttcttgcagg cgtttttcat agcgagcaat ctcttcttca gaagctcctg
    29341 gaagcacctg caccataaag ccaccagcaa ccttgacctt atcattttca tctaaaagca
    29401 cattaagtcc gattgcagat ggtgtttgtt ctgattcggt taagtagtag gcaaaatcct
    29461 caccaatttc tccagtaata agaggcgttg ttgaggtgta agggttacct gtgccataat
    29521 caataatcgt cacaaaatga ccattcccca taaaaggccc aacaaggact tcacctgttg
    29581 cggtcttttt aatatcgaca ccagtatttt gaatgtaacc tttcacatgt cctttggtat
    29641 ctgccacaga gataatgtgt ccaaaagaag aatcaccaat cactttgacg gtgattttac
    29701 tatcgccttt ttgattagcc gccaaaatct ggttggcgat caaggtacgg ccaagcgcaa
    29761 cagtagacga tgacagcgtg ttgtgttttt cttgagctaa tgcaaccgtc tcagtgctat
    29821 caaggacgta agccctaaaa gcccctgatt gtgctattga tttaattatt ttatccataa
    29881 cttagattat agcataaagt gtaaaacttg tattgacagg attacttgaa aaatcaatac
    29941 agatagacag taaaaaatcc cagagaatga ctcgggatta agttaaggtg tggtctaaag
    30001 tcgcgcaatg tggtgacatg gcgcttatgt taattgcttg gttaaatcag cttggaattt
    30061 ttcctcttta acttgatata tcaattcttg gatagataga atcgattcat caaaagatat
    30121 ttcagcaaca gcaattcctt ttgtaagctc atgttgaaca aaaggaatca gttggctgtg
    30181 agtgatgacc aaatctggct ttaaatcagg aatttgataa acattatctt gcaataaata
    30241 ataggaatga aaattaatgc ttttatccga gaaatacctt gggaaagaat ctgttaggag
    30301 atgagcattg ataaaattac tggctacgaa aacaacaact aaaggaggtt ggatatttct
    30361 tagaatttgc tcgacatagt ggcaaaaaag atgaaaatgc ttatggttca agtaacgctt
    30421 accaggaagt ttggccatcc actcttcgac aattaacttt aaggacgtat agagtttttg
    30481 gtttccttta tagtacggag aaatgaataa gttggtctca ggaataaaat gttgcagatt
    30541 aaacaagaat gattttgaaa aaaacataag agcttttact aaactagcct tttgctcttt
    30601 taggttaggt aaaagagtga tgataggatt taaaagcagg cgaaaagtat cattttcttc
    30661 aaaaagttga caacattgtc tgatatgctc aggtgtccat tgtaagctcg caaaagaatt
    30721 attagcggtg atataaatta aatagaggta gtccaaatct cctgctgaaa agtttagttg
    30781 gcagtaagtt tcgataatat cacggctact ttttttcaaa gaatcgtaga caaaaagttt
    30841 ttttaattgt tgaaaaattc tggtttgggg aatagttacc gaaaattgat gccgcttcca
    30901 cgataaagcc aataaaatgt catagaaaga aaacgattcc gataaccaag gagaggtttt
    30961 aaggtgggtg gaactatggg ataaaaagct atgaatagtg tttttgtctt gctgcgtcaa
    31021 gtcataaact ttaatgccaa acttactata tagcagagcg atgaggtaac ggatgcgata
    31081 ttcctcaccg acaatcttgt tcttagagag ttttaattca aagtttctta ataagggaac
    31141 caatgcttcg cgcatccgat aagctgagga gtttgataaa aaatgacttc tcgcaaaatc
    31201 cgtaagggga cgagagtgtg aaccattttt tattaaaaag gctagtaatt gtaagacatt
    31261 agatgatgca tagagttggt aaagataagt ttctttaaaa ggatgtgtaa attggcaaga
    31321 tatcatcctt ttttggatgg tcatagacag actaccaggg aaaaaggcat tcagttcctc
    31381 acaataatgg tttagttgta aaaaggttaa gccagttttt tctgctgcct cagttattga
    31441 caaataagat gtcttaaaaa acaagacaat taactgacat tttgattcga ttgatgattc
    31501 caagtatttt tctatcaagg aaaggggtcc tctcttccta cacttagaaa gccaaataag
    31561 tctttgataa gtgtattctc ctatttcaat tctatctaag ctaaaaatag aaatcaaata
    31621 taatggtttt tggtgaaaag tcgaaacctt ttgaaatagc tttaaagcaa tattttctca
    31681 aaaaatcatt ttcaaaaaca ctaatttggt gaaatataca atcaaaaaaa tatctttctt
    31741 gacaataaca tggtaagctc atatatgttt gtgagaggag agaaaatgaa taacaaaatg
    31801 tttttgaaca aagaagccgg ttttttggca cacacaaaaa gaaaaaggcg atttgctgtc
    31861 actttagtgg gagtcttttt tatgcttttg gcatgcgcgg gtgctatccg ttttggtcaa
    31921 gtagcctatg ctgcggatga gaggactgtg ccgaatttta aaagcccaga tccagattat
    31981 ccctggtatg gctatgatgc ttatactgga gcgtttttaa gatatcatga tcttagagtc
    32041 aatttaaacg gaagtcggag ctaccaagtt tattgtttta atattaaaaa aaactatcct
    32101 cgacctttta cgagtagtga taaaaaatgg tataaaagat tagagggaac tgctgaaact
    32161 tttaaagttc atgctatggc tcccagagta gggggagagg aactaactaa aaaattacgg
    32221 agcgttatgt ataacggcta ccctaatgac ggcaataata ttatgaaagg tttggagcct
    32281 tcaaatgcta tagaagtaac acaatacgca gtttggtact attcagataa gtcacagtat
    32341 gaaattgaga cattatggaa gtctgaagcg aaagaaggta agattagtga gtcacaagtt
    32401 actttaatgc gtgaagccct gaggaaacta atttcctctg atgaagattt ggttaaacaa
    32461 gttccatcga actttaaact tagtactttt gagtcttctg ataagtccta ccaaaatctt
    32521 ttaagtgctg agtttgtacc tgatgatccc cctaaacctg gtgatacgtc agaacataat
    32581 cctaaaactc ccgagttgga tggtagtcct attcccgaag acccaaaaca tccagatgat
    32641 aatttagaac ctacattgcc gccagtgatg ctagatggtg aagaagtccc agaagttcca
    32701 agcgagagct tagaacctgc gcttccccca ttgatgccag agctagatgg tgaagaagtc
    32761 cctgaaaaac ctagtgttga cttacctatt gaagttcctc gttatgagtt taacaataaa
    32821 gaccagtcac ctctagcggg tgagtctggt gagacggagt atattaccga agtctatgga
    32881 aatcaacaga accctgttga tattgataaa aaacttccga atgaaacagg tttttcagga
    32941 aatatggttg agacagaaaa tacgaaagag ccagaagtgt tgatgggagg ccaaagtgag
    33001 tctgttgaat ttactaaaga cactcaaaca ggcatgagtg gtcaaacaac tcctcaggtt
    33061 gagacagaag acacgaaaga gccaggagta ttgatgggag gtcaaagtga gtctgttgaa
    33121 tttactaaag acactcaaac aggcatgagt ggtcaaacaa ctcctcaggt tgagacagaa
    33181 gacacgaaag agccaggagt attgatggga ggtcaaagtg agtctgttga atttactaaa
    33241 gacactcaaa caggcatgag tggtcaaaca gctcctcagg ttgagacaga agatacgaaa
    33301 gagccaggag tgttgatggg aggtcaaagt gagtctgttg aatttactaa agacactcaa
    33361 acaggcatga gcggtttcag tgaaacagtg accattgttg aagatacgcg tccgaagtta
    33421 gtgttccatt ttgacaataa tgagcccaaa gtggaagaga atcgggaaaa gcctacaaaa
    33481 aatataacac ctatccttcc tgcaacagga gatattggaa atgttttggc ctttcttgga
    33541 atccttattt tgtcagtact ttctattttt agccttttaa aaaacaaaca aaacaataaa
    33601 gtctgacgta aaagtgttcc ataagaaata atgcgcaaat atgctgaata gtggataact
    33661 gctacagtca agacaagtgt taattcaaaa ggtactactt atacatctga taataataag
    33721 gtgacagata attcagagca agtaggatgg ctaaaaaatg atgtattcta tgtataatag
    33781 acttacaaga aatcaagtta ttgttaaacg tcgtaaagct taacgctttc aaaagtcgaa
    33841 atcaatcgag actgtcttta ggcagtctcg atttttattg ataaggaact gcaatgacag
    33901 aaagactaaa aaatctaggg atactcttat tatttttatt gggaacagcc atttttcttt
    33961 accctacgct aagtagtcag tggaatgcct atcgtgatcg tcaattgtta tcaacttatc
    34021 acaaacaagt gactcaaaag aagccctcag aaatggagga ggtgtggcaa aaggctaagg
    34081 cgtataacgc taggcttggg attcagcccg ttccagatgt tttttcattt agggatggca
    34141 ttcatgacaa aaattacgaa agcctacttc aaattgaaaa caatgatatt atgggatatg
    34201 ttgaagtgcc ctctattaaa gtgacgttac ccatatacca ctatacgaca gacgaggtgc
    34261 taactaaggg agcaggtcac ttagttggaa gtgccttacc agttggtggc gacgggactc
    34321 acacggttat ttctgctcat agaggccttc catcggcaga aatgtttact aatttgaatt
    34381 tagtgaaaag gggagatact ttttactttc gtgtgttaaa taaagtattg gcttataaag
    34441 ttgatcagat actaactgtt gaaccagatc aagtcacatc tctttctgag gtaatgggaa
    34501 aagattatgc tactctggtt acctgcacac aatatggtgt taataccaag cgtctattgg
    34561 tcagaggtca tagaattgca tttcattaca aaaagtatca acaggctaaa aaagcaatga
    34621 aactcgtgga taaatcaaga atgtggacag aagtagtatg tgctgctttc ggggtggtta
    34681 tagctattat tttagtattt atgtattcaa agatgactga taaaaaagtg attaaaaact
    34741 aacttgccct gaatcttcat tatcgtactg aaactgtcgt aaaagtaaaa taatctggta
    34801 gttactatcc cctttgatga agtttaatta aatctcatca agagaaaaat attaggcggt
    34861 tacaaatgat tttcccaaaa attatttttt ggaacgcata atatggaaaa aaagatgata
    34921 agaacaactg ttttaaacat caaataaatg atatagtagc tatgaaatag cattgtgact
    34981 gtccggtaaa gggatgggac tgaagatttt attcagaagg agagggaatg aataacaaaa
    35041 aattgcaaaa gaagcaagat gctcctcggg tatcaaacag aaagccaaaa caattaactg
    35101 tcactttagt gggagtattt ttaatgctct tagtattgat aggatttgaa ggaaaagttc
    35161 gagcagctca cgaattggtt gaggtaccag tacctatttt tcacaatcca gatccacagt
    35221 cagactatca gtggtatggc tatgaagcct atacaggggg gtatccgaag tatgatttat
    35281 tcaagacata ctatcatgat ctgagagtga atttgcatgg gagtaaaagt tatcaagtat
    35341 attgttttaa cgttcacaaa cactatcctc gtccctccca gagttttgat cggaagtggt
    35401 ataaaaaatt agatggaact gctgagaatt ttgattctct tgctatggaa ccaagagttc
    35461 gtaaagagga gttaaccaaa aaactccgag ctgttatgta taatgcctat cctaatgatg
    35521 ctaatggcat tatgaaagat ttagagcctt taaatgctat taaagtcact caagaagctg
    35581 tatggtacta ttcggacaat tctcctatta acccagatga aagttttaaa tctgaagctc
    35641 tggctaataa aattagtgct acacaaatat ctttaatgag agaggctttg aagcaactta
    35701 ttgctcctga tttagatgag aagcaatcta ggagagttcc tgatggatat cagttgagta
    35761 tttttgaatc tgagaataag gattatcaaa atcttttggc tgcagaagag gttcctattc
    35821 aacctccaaa accgggggat aagccaccac taccgccagt gcaaacaaaa caaacatcag
    35881 tcattatcag aaaatatgcg gaaggtgact actctaaact tctagaggga gcaaccttaa
    35941 agcttgctca aattgaagga agtggttttc aggagaaaat ttttgatagt aacaagtctg
    36001 gagagaaagt agagctacct aatgggactt acgttttaag tgagctaaaa cctcctcaag
    36061 gatatggagt tgccacaccc attactttta aagttgcagc tgggaaagtt ttaataaaaa
    36121 ataaagaagg tcaatttgta gagaaccaaa ataaggaaat cgcagagcca tattctgtaa
    36181 cagctttcaa tgactttgaa gagattggtt atttatctga ttttaataac tatggtaagt
    36241 tttactatgc aaaaaatact aatggaacta atcaagttgt ctactgtttc aatgctgatt
    36301 tacactcacc acctgactca tatgatcacg gagcaaatat tgatcctgat gttagtgaaa
    36361 gtaaagagat aaagtataca catgtttctg gctatgattt gtataaatat gcagtcacac
    36421 caagagacaa agatgctgat ttattcttaa aacatatcaa gaaaattctt gataaaggtt
    36481 ataagaagaa gggggatacc tataagacat taactgaagc tcagtttaga gcagcaactc
    36541 aattagctat ttattactat acagatagtg ctgatttaac aacacttaag acatataatg
    36601 ataacaaggg ttatcatggt tttgataaac tggatgatgc aacattagct gtggttcatg
    36661 agttgatcac ctatgctgag gatgtcacct taccaatgac tcaaaatcta gatttctttg
    36721 ttcctaatag cagtagatac caggcactta ttggaacgca gtatcatcca aatgaattga
    36781 ttgatgttat ttctatggaa gataaacagg ctcctattat tcctattact cacaagttaa
    36841 ctatttctaa aactgttact ggaactattg cagataagaa aaaagaattt aactttgaaa
    36901 tacatttaaa atcttctgat ggacaagcta taagtggaac atatccgaca aactctggag
    36961 aactcacagt tacagatgga aaagctacct tcacattaaa ggatggagaa tcattgattg
    37021 ttgaggggct accttcaggt tactcttatg aaattaccga aacgggtgct tcagattatg
    37081 aggtaagtgt taatgggcag aatgtacctg atggaaaagt gatgaaagaa ctagttaagg
    37141 aagatgagac tgtagctttt gaaaaccgaa aagatcttgt cccaccaact ggtttgacaa
    37201 cagatggggc tatctatctt tggttgttat tacttgttcc atttgggtta ttggtttggc
    37261 tatttggtcg taaagggact aaaaaatgac taattaccta aatcgcttaa atgagaatcc
    37321 actatttaaa gctttcatac ggttagtact taagatttct attattggat ttctaggtta
    37381 cattctattt cagtatattt ttggtgtcat gattattaac actaatgata tgagtcctgc
    37441 tttaagtgca ggtgacggtg ttttatatta tcgtttgact gatcgctatc atattaatga
    37501 tgtggtggtc tatgaggttg ataacacttt gaaagttggg cgaattgccg ctcaagctgg
    37561 cgatgaggtt agttttacgc aagaaggagg actgttgatt aatgggcatc caccagaaaa
    37621 agaggtccct tacctgacgt atcctcactc aagtggtcca aactttccct ataaagttcc
    37681 tacgggtacg tatttcatat tgaatgatta tcgtgaagaa cgtttggaca gtcgttatta
    37741 tggggcgttg cccatcaatc aaatcaaagg gaaaatctca actctattaa gagtgagagg
    37801 aatttaaaaa aatttggaga gagaaaaaat gaaaaaaaac aaattattac ttgctactgc
    37861 aatcttagca actgctttag gaacagcttc tttaaatcaa aacgtaaaag ctgagacggc
    37921 aggggttgtt agcagtggtc aattaacaat aaaaaaatca attacaaatt ttaatgatga
    37981 tacacttttg atgcctaagg ctacatttac ctttgacgtt aaaccggttg atgtaacagg
    38041 agaagaaaag gatacagctt caggcttaaa gattcaaaaa ggtattgctg gagtagcatc
    38101 gcagacaatc caatacgata atactgataa gccaacgaac aaagagaagg cagtaaactt
    38161 tgatttttct acagtaactt ttccaaacgt aggggtttac cgatatgaag tttcagaaca
    38221 agctggtgac gtgaagggga ttacttatga ttctaaaaag tggacagtag atgtctatgt
    38281 agtaaatgaa aataacaagt tcacacctaa atatatcgtc tcaaaagaaa ccacagactc
    38341 aagcaaaaaa ccgattgtct ttaataatga gttaaaaaca acttctttaa ctatcaaaaa
    38401 agaagttacg ggaaacagtg gagataaaac aagtgatttt acatttactc tcctcttgaa
    38461 agaaaatact cagtttgaaa caggtcagaa agtcaaggct actaagaaga agtctggtgc
    38521 tgaagaagaa acagtagaag tcacaattgg taagcaatat acctttactt tgaaagatag
    38581 tgaggagctg atactagata aattaccgat tggtattacc tatcaggtag atgagactga
    38641 taaaaataag gaacagtatg aaacatccgc aaagatgaca gaagattcga aagaagcaca
    38701 aggctatacg ttaaatgatt taaaaacaac agatgagact gctgatacaa tcactgttac
    38761 caataagaga aacttgccta tcccaactgg tgttgtaggc atccttgctc catttgcagt
    38821 tcttagcatt gtggctattg gtggagttat ctatattaca aaacgtaaaa aagcttaagt
    38881 gattagtcaa agaatgatga tgacaattgt acaggttatc aataaagcca ttgatactct
    38941 cattcttatc ttttgtttag tcgtactatt tttagctggt tttggtttgt gggattctta
    39001 tcatctctat caacaagcag acgcttctaa tttcaaaaaa tttaaaacag ctcaacaaca
    39061 gcctaaattt gaagacttgt tagctttgaa tgaggatgtc attggttggt taaatatccc
    39121 agggactcat attgattatc ctctagttca gggaaaaacg aatttagagt atattaataa
    39181 agcagttgat ggcagtgttg ccatgtctgg tagtttattt ttagatacac ggaatcataa
    39241 tgattttacg gacgattact ctctgattta tggccatcat atggcaggta atgccatgtt
    39301 tggcgaaatt ccaaaatttt taaaaaagga ttttttcaac aaacataata aagctatcat
    39361 tgaaacaaaa gagagaaaaa aactaaccgt cactattttt gcttgtctca agacagatgc
    39421 ctttgaccag ttagttttta atcctaatgc tattaccaat caagaccaac aaaggcagct
    39481 cgttgattat atcagtaaaa gatcaaaaca atttaaacct gttaaattga agcatcatac
    39541 aaagttcgtt gctttttcaa cgtgtgaaaa tttttctact gacaatcgtg ttatcgttgt
    39601 cggtactatt caagaataac gaaaggagga gacttttgag aaaatattgg aaaatgttat
    39661 tttctgtcgt aatgatatta accatgctgg cctttaatca gactgtttta gcaaaagaca
    39721 gcactgttca aactagcatt agtgtcgaaa atgtcttaga gagagcaggc gatagtaccc
    39781 cattttcggt tgcattagaa tcaattgatg cgatgaaaac aatagaagaa ataacaattg
    39841 ctggttctgg aaaagcaagc ttttcctctc tgaccttcac aacagttggg caatatactt
    39901 atcgtgttta tcagaagcct tcacaaaata aagattatca agcagatact actgtatttg
    39961 acgttcttgt ctatgtgacc tatgatgaag atgggactct agtcgcaaaa gttatttctc
    40021 gaagggctgg agacgaagaa aaatcagcga ttacttttaa gcccaaacgg ttagtaaaac
    40081 caataccgcc tagacaacct gacatcccta aaaccccatt accattagct ggtgaagtaa
    40141 aaagtttatt gggtatctta agtatcgtat tactggggtt actagttctt ctttatgtta
    40201 aaaaactgaa gagtaagcta taaaacccat ttttaagagt cacacatttt tagttgctct
    40261 atttttataa tgaaacttat tgacatctcc atagtcttgc ttgactttgg agatgttttc
    40321 tatgctgata ggtttttgta ccatataaga caatgattga tgaaaagagt gtcttgaatt
    40381 cttaaatatt atctcggtat ttagcaagat agtcttttga agaaataccc gtgtattttt
    40441 taaagatttt atgaaaatgg gtagtgtcat aaaaatataa gcttttagcc acttctccaa
    40501 caggaattcc tcgttttaag agtagttgag cttctttgat ttttgtactt agaatataat
    40561 gttgtaagga aatatttgag tattttttaa agactgaacg taagtgagat tcggaaacat
    40621 ataggcgctt agcgatatca gataccttta atttggaata caggtgacta tcgacataat
    40681 gaagcacact attgtacatt tgacgtttat cagaaatact tttattggtt aatgattcgg
    40741 aaaaatggat gatagctgca cagcgaattc tcaaaacttt gagcaagtca ctcgcttctt
    40801 catttaaggc catgtagtat ttgacaaggc tataatgctt tgctacgtca gtattttcta
    40861 caaaactgaa ttccagtaac ctagttaaat agataacagt gaggttcttt tccgccctta
    40921 gagcagaaat agaagatgag gtgatacttg ataaggggat acgattgatt tcttgcttga
    40981 gcaattgtgg attgcctagc tttaccagat ccataatatg atttaggtgc tctaaaacag
    41041 gcggtaattg gctagatgct ttgctaatat gcttaaggcg atgaatgata tcaggatcag
    41101 cagtcatctg ttttgaatgc tgcaacaatt gatgaattgt ttgttggcaa gtcgtctcaa
    41161 attgtgtgtc aaaaaaagcg tcaatagcta tcaaaaggtt ccgcacatta ttaattggaa
    41221 aatgtgggac aagtgccata taggataaga gctcttcttt gctacgatga gaagaatgtt
    41281 ttaaaaaatt attagctaat tgttcctgat agtctttatt aagtgaataa gggtagaaag
    41341 gtccaatggc aataatgtag tgacaaagag gaaaaatcat aaaggactct tcaaataaac
    41401 cctcataaaa aataacatct tgtgtggctt gaggaatagc tagatgcttt aaaaattgat
    41461 agtaacataa taaatagtcg tcattaccat atacttgaat aagtgcctta tcttgtgaca
    41521 tcactgaaat aggtaattga gacaagctgt gtaatgtttg cacatgtttt aaatcgaata
    41581 taaccatttg atagattgcc ccccatcctt agttgtatca ttttccttag acttgtaaaa
    41641 gctttagtaa atgttaatga tgaaaattcc tatttggtag gcacactaaa aggtcttcct
    41701 aatagtaaac tctctctcca tcatctcctg ccttaattat actatgaata gctaagcaga
    41761 taaagataaa ggacttgtac gttaagcgct tgaaaaagaa agagttacag ataatgacat
    41821 aaaaaacgct aaaaaaccat caaaataaat actctaacca taagatgtag acttgacaac
    41881 tgaaaatggt aatggtaaaa taactatttg acagttgtcc tgtagtcttt agttttggac
    41941 ataggctgtc gcttatgaat gtggagagag aaaataaatg acacaaaaaa atagctataa
    42001 gttaagcttc ctgttatcct taacaggatt tattttaggt ttattattgg tttttatagg
    42061 attgtccgga gtatcagtag gacatgcgga aacaagaaat ggagcaaaca aacaaggatc
    42121 ttttgaaatc aagaaagtcg accaaaacaa caagccctta ccgggagcaa ctttttcact
    42181 gacaccaaag gatgggaagg gaaaacctgg tcagacattc acttcaaatg ataaaggtat
    42241 tgtagatgct caaaatctcc aaccagggac ttatacctta aaagaagaaa cagcaccaga
    42301 tggttatgat aaaaccagtc ggacttggac agtgactgtt tatgagaacg gctataccaa
    42361 gttggttgaa aatccctata atggggaaat catcagtaaa gcagggtcaa aagatgttag
    42421 tagttcttta cagttagaaa aatcaacaat gtctgttgta tccaagtacg gtgaacagga
    42481 aaaaacatca aacttagcgg atttctaccg taaccatgcc gcctatttta aaatgtcttt
    42541 tgagttgaaa caaaaggata aatctgaaac aatcaaccca ggcgatacct ttgtgttaca
    42601 gctggaccaa cgtctcaatc ctaaaggtat cagtcaagat atccctaaaa tcatttatga
    42661 cagtgcaaat agtccgcttg cgattggaaa atacgatgcc gatagccatc aacttatcta
    42721 tactttcaca gattatattg caggtttaga taaagtccgg ttgtctgcag aattgagctt
    42781 attcctagag aataaggaag tgttagaaaa caccaacatt tcagatttta agagtaccat
    42841 aggtgggcag gagatcacct ataaaggaac ggtcaatgtt atctatggga ataaatcggg
    42901 agacagcaat aattatgtta agaatatatt gagcaatgtg ggtggaagta ttgaaagcta
    42961 caatacacaa acaggcgact ttacttggta tgtttatgtc aatcctaaaa agcaatctat
    43021 caactattct atcctaaatt tatggggatt tggaagggct cgttcaaaca caaacaactt
    43081 agaaacaaat gctaatacaa gtagtgccca attggaaaaa gtagaaattt atgaagttaa
    43141 agaggactat acgctaccta ctagctatgg ggttgatatt acaaaactta ctttaagaac
    43201 ggatatcaca gtggggaata aagatggttt tccaatgact aaacgtcagc ggattgattt
    43261 taaagataat cttaaagatg gaaaaaggtt tgttgttaaa gtaacaggga aaacagacca
    43321 atctggtaag ccactagtcg ttcaatccaa tttagccagt ttttcaacta atcaagatta
    43381 tgttgactta aaaataagtg gagggaatgt ctacttccaa aacgaaattg ccttgtctcc
    43441 ttctaagggt agtggttctg ggaaaagtga atttactaag ccctctatta cagtagcaaa
    43501 tctaaaacga gtggctcagc ttcgctttaa gaaaatgtca actgacaatg tgccattgcc
    43561 agaagcgact tttgagctgc gctcatcaac tagtgacagt caaaaagtag aagcaaaatc
    43621 aaatgaacaa ggagaggttc actttaagga cctgaattcg ggcacctatg acttgtatga
    43681 aaaacaagca cctaaaggct atcagcaggt gacagagaaa ttggcgaccg ttactgttga
    43741 tactaccaaa cctgctgagg aaatggtcaa gtgggaaaag acacatccgt ttgtaaaagt
    43801 agaagttaac aaagaagtca cgattgtcaa ccataaagaa acccttacgt tttcagggaa
    43861 gaaaatttgg gagaatgaca gaccagatca acgcccagca aagattcaag tgcaactgtt
    43921 gcaaaatcgt caaaaaatgt ccgataaagt tcaagaagta acgaaggata acgattggtc
    43981 ttatcacttc aaagacttgc ctaagtacga tgacaagaat caggagtata agtactcagt
    44041 tgaagaagta aaagttccag acggctacaa ggtgtcgtat ttaagaaatg atatatttaa
    44101 caccagagaa acagaatttg tgtttgaaca gaataacttt aaccttgaat ttggaaatgc
    44161 tgaaataaaa ggtcaatctg ggtcaaaaat cattgatgaa gacacgctaa cgtctttcaa
    44221 aggtaagaaa atttggaaaa atgatacggc agaaaatcgt ccccaagcca ttcaagtgca
    44281 gctttatgct gatggagtgg ctgtggaagg tcaaaccaaa tttatttctg gctcaggtaa
    44341 tgagtggtca tttgagttta aaaacttgaa gaagtataat ggaacaggta atgacatcat
    44401 gtactctgtt aaagaagtaa ctgttccaac aggttatgat gtgacttact cagctaatga
    44461 tattattaat accaaacgtg aggttattac acaacaagga ccgaacctag agattgaaga
    44521 aatgcttccg ctagaatcag gtgcttcagg tggtaccact actgtcgaag actcacgccc
    44581 agttgatacc ttgtcaggtt tatcaagtga gcaaggtcag tccggtgata tgacaattga
    44641 agaagatagt gctacccata ttaaattctc aaaacgtgat attgacggca aagagttagc
    44701 tggtgcaact atggagttgc gtgattcatc tggtaaaact attagtacat ggatttcaga
    44761 tggacaagtg aaagatttct acctgatgcc aggaaaatat acatttgtcg aaaccgcagc
    44821 accagacggt tatgagatag caactgctat tacctttaca gttaatgagc aaggtcaggt
    44881 tactgtaaat ggcaaagcaa ctaaaggtga cgctcatatt gtcatggttg atgcttacaa
    44941 gccaactaag ggttcaggtc aggttattga tattgaagaa aagcttccag acgagcaggg
    45001 ccattctggc tcaactactg aaatagaaga tagcaagtct tcagacgtta tcattggtgg
    45061 tcaggggcag attgtcgaga caacagagga tacccaaact ggcatgcacg gggattctgg
    45121 ttgtaaaacg gaagtcgaag atactaaact agtacaatcc ttccactttg ataacaagga
    45181 atcagaaagt aactctgaga ttcctaaaaa agataagcca aagagtaata ctagtttacc
    45241 agcaactggt gagaagcaac ataatatgtt cttttggatg gttacttctt gctcacttat
    45301 tagtagtgtt tttgtaatat cactaaaaac taaaaaacgc ctatcatcat gttaagataa
    45361 gctgttggtg acaatagcga aaaagggtcc acttgttagt ggacctcttt ttctggaatc
    45421 gatcgtctcg gttcactaga taagtcattt taagtagtca aaataggcag agccttaaaa
    45481 ggttttctgc ctttttaatc ttgttgggca cttacgaaag tatttcggcc acttcaagca
    45541 taataaaaag agtctgagca atcatgttgt ctcagactct gactgtaatt atgcaaaaga
    45601 tgatggactg gcagcttcat ttactcaccg aactcccctt tataacttgg gaatgatgct
    45661 gctaatgggt tattttgttt aatacctgct ataaacggta atttattcca tttatctttg
    45721 taaccttctt caagctcagt tttaaacttc caaaccattt tatgagaaaa ataagtgtgt
    45781 gattgacggc cgtaggtgtc tactaaaacg tgagaaaatt ttccgtcgtt ttttagtttg
    45841 taaaatttat ctagagaagg actccaacca gtataaccag ggaatttatc aaattcttga
    45901 ttaaaatttg ttttttggtt ttcccaatca taatgccatt gtgtattact gcgtgtttta
    45961 gaatcaactg taccgtccca ataatgatgc tcataagtta tctccttagg agcttttaca
    46021 cccttaggaa gaaacactga gaaatcaggg taaacccatt gttctttagc tgaattaaag
    46081 gttacagtcc attcgatact gttattgtta tcgaccaatg ttttagtata gtcgacatag
    46141 ttatcaacat agtaatttcc ctgccagtaa aggtgcactt tacctttctc atcaatgtta
    46201 tcatgggcat gtactgattt atatgaacta aacagaatcg aaactgctag tagtgaagtg
    46261 aagagatatt tattacatcg tttcatatca tgaa
//
LOCUS       DAUGFP010000010        46209 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.010, whole genome shotgun sequence.
ACCESSION   DAUGFP010000010 DAUGFP010000000
VERSION     DAUGFP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46209
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.010"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            264..1037
                     /locus_tag="VH663_000558"
     CDS             264..1037
                     /locus_tag="VH663_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ8173780.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLETLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
     gene            1687..1974
                     /locus_tag="VH663_000559"
     CDS             1687..1974
                     /locus_tag="VH663_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible protein J"
                     /protein_id="HEQ8173781.1"
                     /translation="MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNV
                     VVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            1964..2299
                     /locus_tag="VH663_000560"
     CDS             1964..2299
                     /locus_tag="VH663_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ8173782.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQHKMEQIISD
                     IEKLEVFPEVGFDADEKYGSKISHYHSTKGYTLSKDYIVLYHIEGEENRVVIDYLLPT
                     RSDYIKLFK"
     gene            2451..2921
                     /locus_tag="VH663_000561"
     CDS             2451..2921
                     /locus_tag="VH663_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ8173783.1"
                     /translation="MEEFTKPTHKTYSEIFEKWYQAYHDTVEPTTASRTLDLFRLHIL
                     PVMGELPINKTSPLDCQKFITDKAKTFKNIKQIKSYTEKVFDFAIKMKLLKHNPMAEI
                     IMPKRKKIRIENYWTVQELQEFLAIVLQKEPYKHYALFRLLAYSGLKERRAMRS"
     gene            2884..3432
                     /locus_tag="VH663_000562"
     CDS             2884..3432
                     /locus_tag="VH663_000562"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ8173784.1"
                     /translation="MAALRKGELCALKWVDIDFQSETLSVNKSLGRLDGKTIEKSTKN
                     DFSVRKIKLDSETISILQEWKAVSQKEKTQLAVAPLSIKSDFIFTYCTREGSIEPLHA
                     DYINNILSKVIKQHSLKKISPHGFRHTHATLMIEIGVDPVNTAKRLGHASSQMTLDTY
                     SHATAVGEDKSIKQFANYLKNN"
     gene            complement(3564..3956)
                     /gene="rpsI"
                     /locus_tag="VH663_000563"
     CDS             complement(3564..3956)
                     /gene="rpsI"
                     /locus_tag="VH663_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEQ8173785.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3977..4423)
                     /gene="rplM"
                     /locus_tag="VH663_000564"
     CDS             complement(3977..4423)
                     /gene="rplM"
                     /locus_tag="VH663_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEQ8173786.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(4641..4847)
                     /locus_tag="VH663_000565"
     CDS             complement(4641..4847)
                     /locus_tag="VH663_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8173787.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEADDSR"
     gene            complement(4844..5350)
                     /locus_tag="VH663_000566"
     CDS             complement(4844..5350)
                     /locus_tag="VH663_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173788.1"
                     /translation="MFDSKQNLAKSQWGFIVITAVMDLVVIVATLLAKTSFQRYLVGG
                     VAFVLTSFLILLVWGLKTAKKLQTRLDSKEVLDSKVRDDQKLPKYDERQKQILLKGYT
                     IGFWFMIVVIWLSLFISRFTEGLVSASFLFTLALWGGLAVQTTYCNLSMGASIFYRHY
                     LDAKEADE"
     gene            complement(5486..6346)
                     /locus_tag="VH663_000567"
     CDS             complement(5486..6346)
                     /locus_tag="VH663_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ8173789.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHAKNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(6443..6961)
                     /locus_tag="VH663_000568"
     CDS             complement(6443..6961)
                     /locus_tag="VH663_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEQ8173790.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKTNQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     IQLKDFMSQLDR"
     gene            complement(6965..7711)
                     /gene="rlmB"
                     /locus_tag="VH663_000569"
     CDS             complement(6965..7711)
                     /gene="rlmB"
                     /locus_tag="VH663_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEQ8173791.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKSRGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            complement(7758..8555)
                     /locus_tag="VH663_000570"
     CDS             complement(7758..8555)
                     /locus_tag="VH663_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173792.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASMRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAT
                     TNSVDVDTKAQTNQSANQEIDSTSNPFRSATNHRSTSLKRSTKNEKLTSTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLLGLVVSLAAGLFIAIQKVSRRK"
     gene            complement(8751..9164)
                     /locus_tag="VH663_000571"
     CDS             complement(8751..9164)
                     /locus_tag="VH663_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEQ8173793.1"
                     /translation="MTNPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            complement(9157..10500)
                     /locus_tag="VH663_000572"
     CDS             complement(9157..10500)
                     /locus_tag="VH663_000572"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEQ8173794.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEAAGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKLAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFVNYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKALHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFATADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            complement(10528..10758)
                     /locus_tag="VH663_000573"
     CDS             complement(10528..10758)
                     /locus_tag="VH663_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173795.1"
                     /translation="MNKIRVGLYVIMSLSILVFSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            complement(11236..11817)
                     /gene="cysE"
                     /locus_tag="VH663_000574"
     CDS             complement(11236..11817)
                     /gene="cysE"
                     /locus_tag="VH663_000574"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEQ8173796.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTIRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            complement(11826..12578)
                     /locus_tag="VH663_000575"
     CDS             complement(11826..12578)
                     /locus_tag="VH663_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEQ8173797.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSVALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMARFKEEQESAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDNNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            complement(12571..14703)
                     /gene="pnp"
                     /locus_tag="VH663_000576"
     CDS             complement(12571..14703)
                     /gene="pnp"
                     /locus_tag="VH663_000576"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEQ8173798.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIIAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAINRTKEIIAGLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKI
                     DEKGRVDASIKALIPRPPKPEKKEEKHD"
     gene            complement(14983..15714)
                     /locus_tag="VH663_000577"
     CDS             complement(14983..15714)
                     /locus_tag="VH663_000577"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEQ8173799.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPLIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTAGVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEGVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDAKH"
     gene            complement(15724..17103)
                     /locus_tag="VH663_000578"
     CDS             complement(15724..17103)
                     /locus_tag="VH663_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEQ8173800.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLTFARFTRFKYIFLTGHHSFFMACLLSAVLGAVGFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVIFYLVATVASVLRNASVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSEEI"
     gene            complement(17130..17414)
                     /locus_tag="VH663_000579"
     CDS             complement(17130..17414)
                     /locus_tag="VH663_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ8173801.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKDADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            complement(17407..19467)
                     /locus_tag="VH663_000580"
     CDS             complement(17407..19467)
                     /locus_tag="VH663_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ8173802.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVISGSWVFSYLLHELGLPDQTKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEAVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKKQIKQDYESLFYLVKRSLSPLEKQLGKLVNEDEVAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     AKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLIGNHLTIEKGGLGLLDLLTADFIRQADAVSEWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGARQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            complement(19819..20088)
                     /gene="rpsO"
                     /locus_tag="VH663_000581"
     CDS             complement(19819..20088)
                     /gene="rpsO"
                     /locus_tag="VH663_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEQ8173803.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(20264..20464)
                     /locus_tag="VH663_000582"
     CDS             complement(20264..20464)
                     /locus_tag="VH663_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173804.1"
                     /translation="MTMLGYRLSAEVLGSAILPLLAGYLIRQAIAQVYSDLKTAFWLF
                     GLASSVAYGGLVKAKRAHSMTR"
     gene            20590..20847
                     /locus_tag="VH663_000583"
     CDS             20590..20847
                     /locus_tag="VH663_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ8173805.1"
                     /translation="MTQNVCQQNITYTVKERFASYILKAQANQEVHLNFTLLANRFGI
                     SDRHLKHVLKQLIFQRIIERICFKTCRIADLDALTQLSVNN"
     gene            20973..21587
                     /locus_tag="VH663_000584"
     CDS             20973..21587
                     /locus_tag="VH663_000584"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ8173806.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(21620..22162)
                     /locus_tag="VH663_000585"
     CDS             complement(21620..22162)
                     /locus_tag="VH663_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ8173807.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANAPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETKANLLSQAEALLAAI"
     gene            complement(22293..22721)
                     /locus_tag="VH663_000586"
     CDS             complement(22293..22721)
                     /locus_tag="VH663_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ8173808.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            complement(22831..27228)
                     /locus_tag="VH663_000587"
     CDS             complement(22831..27228)
                     /locus_tag="VH663_000587"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEQ8173809.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLTRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRKNHLPNLVKQLEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVKKAVQDNLEAQKSLEAMMPPVEEATPAPKFDYKER
                     AAKRQAGFEKAAITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHDRKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQGAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLARYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRAKKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVQNTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVVPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     ILGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEIGILGNMPEDNQLSLFDDFF"
     gene            complement(27482..29338)
                     /locus_tag="VH663_000588"
     CDS             complement(27482..29338)
                     /locus_tag="VH663_000588"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEQ8173810.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRNAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPART
                     DLDRWVVLDKSIASMDDIPKEVLEEIKAELAAWMISGEDTIAYSTKSSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRTVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH"
     gene            complement(29536..30795)
                     /gene="rseP"
                     /locus_tag="VH663_000589"
     CDS             complement(29536..30795)
                     /gene="rseP"
                     /locus_tag="VH663_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEQ8173811.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLEETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMPDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHT
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            complement(30868..31662)
                     /locus_tag="VH663_000590"
     CDS             complement(30868..31662)
                     /locus_tag="VH663_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEQ8173812.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            complement(31675..32424)
                     /locus_tag="VH663_000591"
     CDS             complement(31675..32424)
                     /locus_tag="VH663_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEQ8173813.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNRRQRRFGKV"
     gene            complement(32640..33005)
                     /gene="yajC"
                     /locus_tag="VH663_000592"
     CDS             complement(32640..33005)
                     /gene="yajC"
                     /locus_tag="VH663_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEQ8173814.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            complement(33121..33495)
                     /locus_tag="VH663_000593"
     CDS             complement(33121..33495)
                     /locus_tag="VH663_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEQ8173815.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKDVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            complement(33610..37107)
                     /locus_tag="VH663_000594"
     CDS             complement(33610..37107)
                     /locus_tag="VH663_000594"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEQ8173816.1"
                     /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSDDGFQSSSKEAKPVLDSSSANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDKDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTRVVGQADL
                     TKSDKGVWRAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLGFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTKVS
                     DDKVPNKATLIDAVAQYPYFIHDSYDSSDAVNHFDWAKATDSRAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTDNHQTKTPDSSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            complement(37305..38918)
                     /locus_tag="VH663_000595"
     CDS             complement(37305..38918)
                     /locus_tag="VH663_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEQ8173817.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTNGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEDLRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDLEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAKELDVPALKAIFSKWQTKLKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSEAKETWLPVNPNYQDINVADALADPDSIFYTYQK
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFAKDLAGAQ
                     VIITNTDVDTVLETKHLQPWDAFCLKLKA"
     gene            complement(39048..40181)
                     /gene="ugpC"
                     /locus_tag="VH663_000596"
     CDS             complement(39048..40181)
                     /gene="ugpC"
                     /locus_tag="VH663_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEQ8173818.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLAIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            complement(40479..41327)
                     /locus_tag="VH663_000597"
     CDS             complement(40479..41327)
                     /locus_tag="VH663_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEQ8173819.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLFETSQFIYLNHQQALPADLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAISIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQFLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLYLKQLDDLAFAH
                     LFLLKY"
     gene            41668..42990
                     /gene="ska"
                     /locus_tag="VH663_000598"
     CDS             41668..42990
                     /gene="ska"
                     /locus_tag="VH663_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEQ8173820.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGPEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSQPAQGGKTEQDLNPKSKPLATDKGAMSHKLEK
                     ADLLKAIQEQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADRDDSVTLPTQPVQE
                     FLLSGHVRVRPYQPKAVHNSAERVNVNYEVSFVSETGGLDFTPLLRNQYHLTTLAVGD
                     SLSSQELAAIAQFILSKKYPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQ
                     AYEINPKTGIKEKMNNTDLISEKYYVLKKGEKPYDPFDRSHLKLFTINYVDVNTNELL
                     KSEQLLTASERNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHDDTNRIIT
                     VYIGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPNDK"
     gene            complement(43088..43531)
                     /locus_tag="VH663_000599"
     CDS             complement(43088..43531)
                     /locus_tag="VH663_000599"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEQ8173821.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     SQLYDSFNEQLAEFVPVERGVFGADMQVSLVNDGPVTIILDTKCH"
     gene            complement(43546..45765)
                     /locus_tag="VH663_000600"
     CDS             complement(43546..45765)
                     /locus_tag="VH663_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEQ8173822.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKADVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGQDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLTHLTTVVEKIKAVPDVYSVKRTNG"
     gene            complement(46017..46127)
                     /locus_tag="VH663_000601"
                     /pseudo
     CDS             complement(46017..46127)
                     /locus_tag="VH663_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_129544913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT        13618 a        10183 c         8688 g        13720 t
ORIGIN      
        1 atttttttcg tttttatgtt attatcctaa tgtttcttgt gtttcttttt catttcataa
       61 atccctttga cagccactcg taacctatca tatattacga aacccttatc tctatttatt
      121 gcaaatgttt tatttaaaac attttctatt ttgttaattt ttgttttttc caatgtttat
      181 gtttgttttg tttgattttt ttattgatat tatgttatga tacctttaat agttataccc
      241 taagacactg ataggagata agaatgaaaa agaaagagcg tcatgaaaaa attctcgata
      301 tcttaaaagt tgacggtttt ataaaagtaa aagatattat cgatgaaatg aacatctcag
      361 atatgaccgc tagacgagat ctcgatacat tggcagataa aggattactt atcaggaccc
      421 atggtggtgc tcaatatttg gattattctt cagcaaagga cgaaggtcac gagaaaacac
      481 acactgaaaa gaaggtctta cagactaccg aaaaaaaaca aattgcacaa agagctaaaa
      541 acattattaa agatggcgaa actgtcttta taggtccagg gacaacatta gagcatctag
      601 ctgtcgaatt aaaaaataga aatatcaggg tcattacaaa tagccttcct gtttttttaa
      661 ttttgcaccc gagcactact attgatttga ttttaattgg tggtgaatgt cgtgaaatta
      721 ctggagcttt tgttggtgct attgccatta ataacctaga aacattgaca ttttcaaaag
      781 cttttgtcag cgcaaatgca gtttacaata attctattgc gacgtacagt gatctcgaag
      841 gagaagttca aagggttgca ttgaataatg ctgttgagaa aatccttcta gttgatagta
      901 ccaagtttga aagttatgac ttctataatt tctaccaact agatcaagtg gacttaattg
      961 tgactgataa aaatgtgaca actgatatca tagaaaagta caagaaattc tcaaaaatca
     1021 taatcgctga caagtaaaag acctactcat attaaaaagc acttcagggt ttagatatat
     1081 cccctgtcaa gtaaacaggg agcatatctg aaagtagtgt ttgtgttata aagaagtttc
     1141 ttgttaactt attttctggt ttttttcaga cttatcagtc ttgtactgtt cctttttctg
     1201 atgaataacg tggaaatagg cagacagcta tatatttcac ctctagaatg gttgccatta
     1261 ctgtcatcaa gacatgcttg ttctttatcg tatggagtga agctttcttt tagacatcaa
     1321 aatacctctt aagtagattt tggttcgtta ccatgtctac ttaagaggtg tcttcaaagc
     1381 gctttaaaac gttgatataa cagtatatta acgttttgag aattgtgaag ctttacgagc
     1441 ttttttaaga cctggttttt ggttcactcc attatttaaa gtcaaatccc tttaaaatca
     1501 atgtttctaa aatgaaaaat cactaaaaat cttatttata gacaaatact taaacctaaa
     1561 cggtatcaaa aataagtttc ggagcttatc aaataatgaa aatctgtctc aattaccgag
     1621 tcttttttat tgactttttt gtatatatga tataattaaa taaattacat aaaggggtta
     1681 cataaaatga ctacagtaaa aaaaaacaga gcggttacct ttcaagctaa taaagaattg
     1741 gtaagcgaag caatgacagt attaaacaaa aaaaatttaa ccttatcatc tgctttaaga
     1801 ttattcctac aaaatgtcgt tgtcacaaac gaggttgact tattgacgga agaggagtta
     1861 gagaaagaaa aacttttcaa gcaatttcaa gctgaaatca acaaaaatat tgaggatgtt
     1921 cgtcaaggga agttttatac ctctgaagaa gtgagggctg aacttggact ataagaaata
     1981 tcagattatc tatgctcctg atgttttaga gaagctaaaa gaaattcgtg attatatttc
     2041 tcaaaactat tcctcaacat caggtcagca taaaatggag caaatcatta gcgacataga
     2101 aaaactcgag gttttcccag aagttggttt tgatgctgat gaaaaatatg gttcaaaaat
     2161 cagccattat cacagcacta aaggttacac cttaagtaaa gattatattg tcctatacca
     2221 tatcgagggg gaagaaaata gggtcgttat tgattacttg cttcctactc gaagcgacta
     2281 tataaagtta ttcaaataac acgttaaaag ccttagcagt cttgctaggg cttttttggt
     2341 agattacaaa ttaactagga gcaaaatgag ttcgggctgt ttagtagata ttccacaaag
     2401 aagccaaaga ctacgaagct aggctaatct accttaaggc ttcaggaaac ttggaggagt
     2461 tcaccaagcc aactcataaa acttatagcg agatattcga gaaatggtat caagcctatc
     2521 atgatacggt agagccaacc acggcttctc gtactcttga tctgtttcga cttcacattt
     2581 tgccagtcat gggagaattg cccattaaca aaacctcccc tttagactgc cagaaattca
     2641 tcaccgataa agcaaagacc tttaagaata tcaagcaaat caagtcctac actgaaaagg
     2701 tctttgattt tgccatcaag atgaaactgc tcaagcataa ccctatggct gaaatcatta
     2761 tgccaaagcg gaaaaaaata cggatagaaa actattggac ggttcaagaa cttcaggagt
     2821 ttttagccat agtacttcag aaagagcctt acaagcacta cgcactattt cggctactag
     2881 cttatagcgg ccttaaggaa aggcgagcta tgcgctctta aatgggttga tatagacttt
     2941 cagagtgaga cactaagcgt caataagagc ctaggacgat tagacggaaa gacaatcgaa
     3001 aaaagcacta aaaatgattt ctcagtaaga aagattaagc tagacagcga aactatttct
     3061 attctgcaag aatggaaagc cgtcagccaa aaagaaaaga cacaactagc agttgcacct
     3121 ctttcaatta agtctgactt tatatttaca tactgtacta gagaagggag tattgaacca
     3181 cttcatgcag actatattaa caatatctta tcaaaggtca ttaagcaaca tagtcttaaa
     3241 aaaataagtc ctcacggctt caggcatacc catgcaaccc tgatgattga gattggagtt
     3301 gacccagtaa atacagccaa aaggttagga catgctagta gtcaaatgac tcttgataca
     3361 tacagtcatg ctactgcagt cggagaagac aaatcaatca aacaatttgc taactatctc
     3421 aaaaataact aaataaacgt attaaaccaa gtcaaaagtt ttccgaaacg gtatcaaaaa
     3481 aggtatcaaa agcaaaaaac gacaaaaaaa gcaccttgca agagacttgc aaaatgcttg
     3541 taatcgttga tgtaaagctg ttcttaacgt tttgagaatt gtgaagcttt acgagctttc
     3601 ttaagacctg gttttttacg ttcaaccata cgtgcgtcac gtgtaagaag gccagcgcgt
     3661 ttcaatgaat cgcggaagtc tgggtctact tgaagaagcg cacgagcgat accatgacgg
     3721 atcgcacctg attgtccacc gtaaccacca ccaacaacgt taacgaaaac gtcgtatgaa
     3781 ccttcagttg atgtaactgc aaaaggttgg ttgatgatca aacgtaagtc agcatgtggg
     3841 atgtattctt ctacatcttt tttgttaaca gtgattttac cagtacctgg aaccaaacga
     3901 acgcgtgcaa cagcgttttt acgacggcca gtacctgcat attgtgcttg tgccattatt
     3961 taatgctcct ttcctcttag ataagtcctg agatgtcaag tacttctggt tgttgtgctg
     4021 cgtgagtatg ctcaccgcca acgaagactt tcaatttcat accttgtgca cgtccaagag
     4081 tgttatgtgg aagcatgcct ttaactgatt tttcaatcaa gcgaactgcg tttttagagc
     4141 gaagttcacc agcagtgatt gatttcaaac cacctgggta cattgagtga gtgtagtaaa
     4201 ctttatcagt tgctttttta ccagttaatt taactttttc agcgttgata acgataacaa
     4261 aatcacctgt atcagtatgt ggtgtgaaag ttggtttgtt ttttccgcga agtacgctag
     4321 caactactgc agaaagacgt ccaagtggca catcagttgc gtcaacaacg taccatttgc
     4381 gttcaacttg gcctggttta gccatgaaag ttgttttgtt catgatttct cctatacgaa
     4441 ttcgtaatat ttgtttacaa ggtgatgggt gttccgtccc atcgaaggta tttggaaggt
     4501 tccggggcct ttcaaatggg gtaaacaata ccgcctacta tgatatcaaa cttaagaggc
     4561 tccagtcaag tgctttagct gattttattg gattattttt tctttcgatt taggagaatg
     4621 gcagagctgc tctgctggtg ttaacgagaa tcatcagctt cccaaaaaag gtcatctaaa
     4681 gtcttatcaa gcactcgaca aatggcaatg caaagattaa tcgttgggtt gtaatcacct
     4741 ttttcaacag ccgcaatggt ttgtctggag acgcctacca aatctgctag agcttgttga
     4801 gacaaatctt tgcctgcacg cgctgctttt aatttgaggt tcttcattca tctgcctctt
     4861 tagcatccag ataatgacga tagaaaatac tagctcccat actaaggtta caatatgtcg
     4921 tttgaactgc caaccctccc caaagagcca atgtaaagag gaagcttgca gataccagtc
     4981 cctctgtgaa tcggctaata aagagtgaca gccagataac cacaatcata aaccaaaacc
     5041 caatagtgta tcctttgaga agaatctgtt tttggcgttc atcatatttg ggcaacttct
     5101 gatcgtccct taccttagaa tctagtactt ccttgctgtc tagtctcgtt tggagctttt
     5161 tcgctgtctt tagcccccat accaacaata tgagaaagct agtcaataca aaagctacgc
     5221 ctccaactaa gtaacgttga aaactggtct tagccagtaa tgttgctact atcacaacta
     5281 gatccatcac tgctgttatg acaatgaaac cccattgtga cttagcgaga ttttgtttgc
     5341 tatcaaacat gattcgtttc tcctttaaat ctttatatga cataatataa attattttgg
     5401 atattttgtc aaatatattt tacataattg attgacttaa gcagcaaaaa aggattgagg
     5461 atttctcttc aatccttata agggattaac gggaattctg cccaatcaca aaaactgcca
     5521 gggtgttagg accaacgtga gctgagataa ctggtcctag tggcatcata agaacatcgc
     5581 taatattctc atgcgccagc agctcttctc gcaacttttc ggcactaccc tgatcgcttg
     5641 tataagagac aataaccgtc gaatccgcaa tatctttttc cacttgagcc accatttctt
     5701 tgatggcttt ttggcgtccc cgaatttttg caatgggcac caatttccct tcctcatcaa
     5761 tccacaaaag aggtttgata ctggctaaac tgcctagaaa agctgaacct tttgacagac
     5821 gcccaccacg catcaaatga aaaagatcat ctactaagaa ataggtgcgc agtcgaggaa
     5881 tcactgcttc aacaatatct ttagtctcca gtaaattttt gccactatct ctggcttctg
     5941 ctgctaaaat ggttagatag ccttctcctc ctgcagcagc caaggtatca acaatttcaa
     6001 tcaccgcatc gggataatct tctcgaacaa gatcgcgtgc cattagagcg ctttgatagg
     6061 tacccgataa aaccgaagaa aaagcaagat aaagcagtgc tttgttgttc ttagcatgtt
     6121 cacgaaaaac cttctcaaac tctcccacat taatctgact ggtttgagga tgactgccag
     6181 ctttcatctt tttcaaaagg taatcactac taatacggtt aggaccgacc gtttcataaa
     6241 cctctccatc gcacaaaatc gttaatccta taaggacaat atcatgatct tctgcccagg
     6301 tttgattcaa atcagcggtt gaatctgtca ttattgtaaa ggtcattctt acttctcctc
     6361 atccagtttt ttagtttgat aatcaaataa ttattgtttt attttatcaa aaaaacccgc
     6421 acttggcgag tcgtaattct cattatcgat ctaactgaga cataaaatcc ttcaattgaa
     6481 taagctgtcc ttggtcaaaa ggccgcagtt taggcgtttt gagatcaata tctctagaca
     6541 ttttatctaa atcagctttg acagtatgaa ctctttgctc taattctctt gcagtgacac
     6601 gaagagctcc ttgagaaaaa atcgtccatt gttcatttaa atcactcgta gctacttcta
     6661 ccatatgtat agccgtattt aactctgctg ccatgcgctc aatgtagcta tctgctgttt
     6721 cgtcttcctc tgtaaatacc actgagatat aatactggtc atatcgttgc cttaaccccg
     6781 gtacatattg ggcatcaaaa acacaaataa tattaatatt ctcaaaatgg gcataatgat
     6841 taagttttgt taaaagtgtg ttacgtgcct gatcaagctg atttgtctta aacaactgac
     6901 gggttgattg ccaaaaggca atcatattat acccatctac taataatatc cgttttttca
     6961 taatttagcg cctatttcgg aaaacttcat acatgagaat agctgccgct acgctagcat
     7021 ttaagctctg tacgtgacca ttcataggaa tcgtaatcat ttcatcaact tgctttttga
     7081 tattggtaga gatgcctttg ccttcgttac caatgactaa agcaagttta ccattagtgt
     7141 tccagcaatc agacggtgtt ccattcatgt ctgtgccaaa aatccagaat cctcttgatt
     7201 tcaatttatc tagagtttga ctaaggttag ttactctcgc tatcggaata tgttcaacag
     7261 cacctgtaga cgtctttgaa accactggag tcacaccaac agaacgatgt ttaggaataa
     7321 tcactccaca tacatttgta gcatcagctg tccgtaaaat cgatcctaaa ttatgaggat
     7381 ctgtcagacc atctaaaata agaatcaaag gatttgcttc ttgctctgct atttcgagga
     7441 tctcatcaac atcagtatag gcaaaagctg atactctcag aacaaaacct tgatgcacag
     7501 ctccatcagt catttgtgac aaggtttttt taggcgtcca tgaaatagcg accttttttt
     7561 gtgtcgctaa gcttttgatg ttatccactt tctttcctct taaatcctct tggatataaa
     7621 gcttatttcc tgtatttgct tgaaggcttt ctgtaacggc atgaacacca tagacgatat
     7681 cgtttgtttc aatagtatct ttatcttcca tgagtttatt ataccatgaa tgcttcaaaa
     7741 taaatgcata gactctttta ttttcgtcta gatacttttt gaatagctat aaataaacct
     7801 gcagctaaac tgactactag acctaataag acccaaggga atcctctttt tgaagtaggt
     7861 tttacttcct ctttgtccac tagcattttt gtcttgttgc taccgttttt ttggctatta
     7921 ctagcagttg atgtaagttt ctcattttta gtagatcgct ttaaggaagt tgatcgatga
     7981 ttagtagctg atctgaaagg atttgaagta gaatctattt cttggttagc tgattgattt
     8041 gtttgagctt ttgtgtctac atcgaccgaa ttcgtcgttg ctttagaagt atccttttgt
     8101 tgcatattag ttggtgtttt tctagctttg ttttgatcaa aatgaacagt tcctgattga
     8161 ccagttactg ccttttcttg agtatgaacg gtgacagtta catgatagtc accactcttt
     8221 tgtccagtta aatcaaacca agctgtaaaa cgacgctgtt gcgctgccat agtatatacc
     8281 caactattaa tagtattatc actagcgtct ttaagtgtta tcgttgcctg agttggttgt
     8341 ctgacaggtt gatctgtggt gactgtagca tcaatataag gttctgttcc acgcatactg
     8401 gcttcaacac tgacctgact aatagttgat tcagctataa cattatcagt gtgctgatct
     8461 tgtgcataca cagatatggc tttgccagaa gtccccagca aaccaataag taaagctagt
     8521 gataataagc taactgtttt ttgcatagtt gtcataataa atatctcctc tttaaattag
     8581 tcttaccctc tcattataaa ctattcgaaa atgatttcat tatttttatg tcttttttgt
     8641 tacaaatgta tcttttttat atcattatgt ctttttgatg tcataaaaag agttaatggc
     8701 tctctacctt tacttcagac ctatgaaaat aagagtctcc taggggactc ttatgaggat
     8761 atcaattgtt gcttttctac atactcaata caccatctga tcaattcttc taaccgctct
     8821 ttttggccca tcatatcaag ataacccata atggcttcaa aacctgtcga catacgatag
     8881 gtaataatat cggcattctt agctttagta tggctattgg tattgcgacc acgcttatag
     8941 atgtcttctt ctttttcggt caatagctgc gcttctaaca tagcctgaat caagttggct
     9001 tgtgccttag cagaaacata tctcgttgct aaacggtgta gctggctagg tttcgtttta
     9061 ccttgaaaaa tgagatgacg acgaacatag taggaataaa ccgcatcccc ttcaaaggct
     9121 agggcaatgc cattaatcaa attcacatca actggattag tcacgcagcc acctcacacc
     9181 atctttggta tctagcagct tgatgccttg agctgctaac tgatctcgaa tggcatcagc
     9241 tgtggcaaaa tcacgattcg cgcgtgcttc ttgtcgctta gcaatcaagg cttcaatgtc
     9301 aacctcaagc acctcttctt caaagataat accaaaaaca gctaacattt tctcaaaagc
     9361 gcttttaaca ggttctgtat aggagccaga attaatccac ttggccatgt caaatacgac
     9421 ggtaatccca tttgctgtat tgaaatcatc gtccatagcg tcttggaaag ctattacaaa
     9481 ctgttttagc tcttgctcat ctgcagtttc tgtcagcggt tgttgcaagg tgtttttaag
     9541 atacttcaga ttgatttccg catcatgaag agccttttca gtgaagttaa ttggttttcg
     9601 gtactgttgt gtcgcaagaa agaatcgcaa aacctgacca tcaacggttt gtaacatgtc
     9661 atgaacggtc acaaaattac ctagtgactt ggacattttt tcgttatcaa cagtgacaaa
     9721 cccattgtgc atccaatagt taacaaaagt cttgcctgtt ttggcctcag attgggcaat
     9781 ttcattggta tgatgaggaa attccaaatc agctccgcca ccatgaatat caatggtatc
     9841 accaagaatt tcagtagcca tcacagaaca ttcaatgtgc cagcctggac gaccaaaccc
     9901 ccaggggcta tcccaagaaa cctcacctgc cttagctaat ttccaaagag caaagtccag
     9961 tggattttct tttaaagctg tctcagcatc tgttcgacca ctggctccaa cttcaagttc
    10021 tgacagggtt ttattagcta gcttggcata atgctctgac ttttccacgc ggaaatacac
    10081 atctccagct gcttcgtaag caaaatcttt ttcaatgagg ctttcaacaa atgaaatgat
    10141 ttctgctata taatccataa cacgaggatt ttgtgtggct ggcttaacac caagtgcctt
    10201 ggtatcttct ataaaagctg caataaagcg atctgacaat tctttaggag aaacacctgc
    10261 ttgagtagca gctttgataa ttttatcatc gacatcggta aaattggaaa tgtaattgac
    10321 ctggtagcca gtatactcaa aataacgtcg aatggtgtca aaagcgaccg ctgatctagc
    10381 atttccaata tgaatataat tatagaccgt cggtccacaa acgtacatat tgactgtatt
    10441 ttcagtcaaa ggtacaaact tgcggagcga acgggtcatg gtatcataaa ttttaatcat
    10501 gtaaactcct tattcaatag cctggcatta gttgtcatct tgatatccta agtctttttc
    10561 tgaaatgctg ttccaccttt tcaacaattt gatattaata accaaaccta ttaaccaaaa
    10621 tagggtcaaa atgaagtata taatcgcttt aaccatgtct tgtctcagta aaaaaattag
    10681 actagccacc agtgtaatac taaaaacaag gatagacaaa ctcataatga catacagccc
    10741 aactcttatt ttattcatga ggctccttta ttaacgaaaa accttcacaa cctgtgaagg
    10801 cttcagaaag tagacaaacc cacttttatg ctcaaaaagc atgaatagat ctgctgattt
    10861 gacatcaggt ttcctatttc tgtatgtggg gtgagccaaa acagctgctt tttgttgtgg
    10921 taaaagtggc atcaatactg acagtgatgc ccatacggat gtttgagacc ttaggctcaa
    10981 aactaaggca tggaattcca aaggaagtcg ctgccgtaag caccacctga gaaaaaatat
    11041 cacaatagac tgtttcttca gtctaagact tgcttagttg atgctctttt acgcgttttt
    11101 tgtattttgt acattcccat atcactacac tcagcaacca aactgatagg gctagctaag
    11161 ttcaacatta ctctactaga aagtcccaaa atattttatt ggaaggcatc agtgcttctt
    11221 tttgttattt tccgatcatt ttgacaactg ataagagacc tctcgttgtt tttgtaaact
    11281 ttgaatttga cgattatctt tttgcccatg cactcgtact atcttagctg gcacacctac
    11341 aactgtcacg tcttcaggaa catctgataa cacaacagct gctgccccta ctttagcatt
    11401 tgctccgatg tcaataggtc caatcacttg ggcatgtgcc gaaattaagg caccttgtcg
    11461 aatcgttgga tgacgcttgc cacaatcttt tccggttcca cctagggtca ccccatgata
    11521 aagcatcaca cctttttcaa caattgctgt ctctccaata acaagaccag caccatggtc
    11581 aatgaagact ccaggagcga tttgtgctcc aggatggatt tcaatttgtg tccaaaatcg
    11641 ccaaaattgg ctatgcattc tggctagtaa tttaaaatga tgtcgccaaa gaaaatgaga
    11701 caaacgatga gcagctaggg ctttgatacc tggataagtt aggataacct caagactatt
    11761 acgagcagct gggtctagag ctttaacaat agctatactt tctttccacc aacccatact
    11821 aactcctacg acttatctaa ttctgaccaa tcaacaagaa tttgttggtc atcaaaagga
    11881 ttgatggcga caccgaacgt ttcatcaagc tcgttttcac catttcttgg ttgataaata
    11941 tcatcaatcg tccaagtcag aatcactcct tcagctttcc tgaaaaggcc tccaaaatcg
    12001 tcttggttgt accatttcgc aaagctttga agatttgaaa aggcgggaac atagcttttt
    12061 ccttcaggcg ttgacatggt aggaaaaagg cggtcgtaat ggttattatc tttaggataa
    12121 acaaaggcag gaacaaggta aaccttttcc atcgtatctg ccgccacatt gtcatcactc
    12181 ataagcgtat ttaacacagt ggtgtaatgg ttcataaatt gaatcatatc actgcttttg
    12241 aaaatggtag aattcccaaa atctcccttt ttcttaaggt taaagacaag acctgctgca
    12301 ccagatgtaa tgacttcttc tagcactgca agagctgaac gctccagcca atactgactt
    12361 tgagcactct cttgttcttc tttaaagcga gccatatctt ccttatcggt aaaaacaggg
    12421 gtaaccttga caccttcgac ttctatcacg tagggttctt tagcagccca gacaggaaaa
    12481 ttatggaaag cattgacaag ggcaacacta tccaaaaagt tatcaggcgc attgataaaa
    12541 gctcttaagc gaatatctaa ttcatttgat ttagtcatgt ttttcttctt ttttctctgg
    12601 ttttggtggg cgtggaatca aagctttaat tgaggcatcc acacgaccct tctcatcaat
    12661 cttaatgacc ttaacatcaa cgtcttcacc cacttcaagg acatctgaca cgttggtcgt
    12721 tctagtccaa gcaatttcag aaatgtgaac aagtgcatct gttttgtcaa agaggttgac
    12781 aaaggcacca aatttctcaa tacggacaac cttagcatgg taaacttcgc ctaccttagc
    12841 ttcacgtact aggccagcga tgatttcttt agtgcggtta atggcagctt ggtcacttga
    12901 gtagatagag acatttcctt cgtcatcaat atcaattttg acacccgttt cggcaataat
    12961 cttatcaatg gtttcgccac ctttaccgat aaccactttg attttgtcta cgtcaatctt
    13021 gatggtgtca attttcggtg cagttggggc taactcagga cgtggttcag cgatagttgc
    13081 ttcaatcaca tcaaggattt caaagcgagc ttttttagct tgtgcaagag cttcttctag
    13141 gatttgaggc gtaatccctg caatcttaat atccatttga agggctgtaa tcccttcacg
    13201 agttcctgcc actttaaagt ccatatctcc aaagtggtct tcaagaccct gaatgtcagt
    13261 caagacagta taatttgtcc catcggaaat gagacccatg gcaatccctg ccacaggagc
    13321 cttgattggt acaccaccag ccatcagagc aagggtacca gcacagatag aagcctgaga
    13381 agacgaaccg ttagattcta gaacttcggc aactaaacga atggcataag gaaattcttc
    13441 taagcttggc aagacttgtt caagagcgcg ttcacctaag gcaccgtgtc caatttcacg
    13501 acgaccagca gctccgtaac gacccgtttc accaactgaa tattgcggga aattgtagtg
    13561 gtgtaaaaag cgttttttgt actctggagc cagaccatca atgatttgag tttctcccat
    13621 tggtgccaag gtcaaaactg atagcgcctg agtttgacca cgtgtaaaga gacctgatcc
    13681 atgtactttt ggcaagaagt caaccacagc atcaagcgga cgaatttcgt caatcttacg
    13741 accgtcagga cgaatcttgt cctctgtaat gaggcggcgc acttcagcat gctccatttg
    13801 ttcaaggatt tctgccacgt cacgcataat ggttgctagg ttttcgtctt ctgcgtaacg
    13861 ctcttcgtac tcagccttaa ccatttcttt aacggcttct gtggctgctt cacgcgcttt
    13921 tttctcttca acttgaacag ctttttgcag ttgagcattg tacttagcca caatatcagc
    13981 ttgtaagtct gcatcaacct gtaacagttc aacctcagct ttctctttac caacaactgc
    14041 tatgatttgt tcttggaagg caattaattc ttgaatggct tggtgacctt ttaaaagagc
    14101 ctcaagcatg atgtcttctg ataattcttt ggcaccagac tcaaccatat tgatggcttc
    14161 tttgctacca gcaacggtta attctaagag tgatgcttcc atctgttctt tatctgggtt
    14221 aatgatgaac tcgccatcaa tatagccaac ttgaacacct gcaatcggtc cattaaatgg
    14281 aatatctgag attgacaagg ccagagatga gccaaacatc gctgccattg gagcgctagc
    14341 attttcatca taagaaagga cagtgttaat cacttgtact tcgttacgaa aaccttcggc
    14401 aaacatggga cgaatcgggc ggtcaatcaa gcgagctgtc aaggtcgcat ctgttgaagg
    14461 gcgtccctca cgcttcatga aacctcctgg gaatttacca gcggcataca ttttttcttc
    14521 gtaattaact tgaaggggga agaaatcacc cgttgccatt ttcttagaca taacagctgc
    14581 ggtcaggacg gttgattctc cgtaacgaac aacggttgcc ccgttggctt gcttagcgac
    14641 ttgaccaact tcaacaacaa ggggtttccc tgcaaatgtt gttgtaaagg tttgttttga
    14701 catatattct ccttttaatt ttcagagggg taaagaggta cctgttctct gcttagattc
    14761 agttgttata cgttgttttg ctacaaaaag caacctgtca ggcttgtcac aagctcctct
    14821 ttgtatcaaa cgacgtatct gctacatttt accataaaat agcagaaatt tcactgtcag
    14881 atcgcaatct cttgaaatgt ccttgaaaaa tattaaacga aacttatact ctttaacaaa
    14941 gcaaaaaaag caggttggta tacctgcttt cgtctcctaa tattaatgtt tggcatcctc
    15001 taaaatttta aagccgaggc tggtactgtc tttcagaaac atttgcacgg tttcaaggga
    15061 atagtcttct aaatcttttc cttgaagagg caacttggca atcaaatcag tggtacaagt
    15121 gataatgtcc acgcctagct gatcagcttg atagatatta taagtttcac gaggactagc
    15181 ccacaacaac tcgacccctt ctttttgacg acaaatgcgt agagcttctt ccatgattgg
    15241 cattgggtca acgccggtat ctgcaatacg accagcaaaa acagacacaa tagctggcac
    15301 tccagcggtt aaatgatcaa ccacagcttg agtctgctca attgtaaaaa tggctgtgac
    15361 gtttaactta atgcctttgg ctgacaactt ttgaatgagg ggacaggttg attctcctgt
    15421 tgaagtggtg actggaattt tcacatagac attgtcacct agactggcaa ttttctcagc
    15481 ttccttttcc atgcttgcga gatcatccgc aaagacttca aaggacactg ggtagtctgt
    15541 aattttggca agcacttctt tggcaaagcc aatataagaa ctgatgcctg cttttttcat
    15601 caaacttgga ttggttgtaa agcctgtgac caaacctgtt tgtaaatcct ttaacatggt
    15661 ctctaaaacc gcaccgtctg aaaagacttt aacgtttagt ttttttgtca tcatatcctc
    15721 cttttagatt tcttctgaaa cattattgat ggcctcttga gattttgaca gaaagtttgg
    15781 aagcaccaat acaatagcaa gcacagcaac caccaagtaa ataccgattt gaccaatcga
    15841 cgttcctaag cggcccagta aaatccctaa aacaccgaaa tccacatctc caaaggtcgt
    15901 gtttgaaaaa ccaagtttac caagtacagg tagaagcatg gctggcaaga aggcgagtaa
    15961 taagccatta gcgaaagcac caatcatcgc accgcgacga ccacctgttg aatttccaaa
    16021 aatccctgct gttgcaccgc agaagaaatg aggcaccata cctgggataa tcagaacacc
    16081 acctgcaact cctaaaatca acatcccaag taagccacca acgaaacttg acgcaaaacc
    16141 gatgataaca gcggttggcg cataagggaa gaataccgca caatccacgg ctggaatagc
    16201 atttggaatt aatttgttag caataccttg gaaggctgga atcaagtcag ccaaaatcat
    16261 gcgaacaccg gcgtagacaa ttgccacacc aaccgcaaag gtaagaccac tcttaatagc
    16321 aaaaataaat gggttttgac ctgctgctaa ttcttctgtt actgaagcat tcctcaaaac
    16381 agaagccact gttgccacca agtagaaaat caccatgata agtcctgttg aaatggtggt
    16441 gttgcgtaag aaactccatt tttcagaaat ctgaaggtcc tcggtatctt tactgtcttt
    16501 gccaaccttg ctaccaaccc aggcagaaag gtaatagccc aaactaccaa agtgtcccat
    16561 ggcgatttca tctccgtcag tcactttcaa cgtatactgt tgaccaatgg ctggcgaaat
    16621 agctgaccaa gctcccaaga ggaacccacc taagataatc aaaagacttc ctttgaaacc
    16681 aacagctcca agcacggcgg acaagagaca agccataaag aagctgtgat gtcctgttaa
    16741 gaaaatgtat ttgaagcggg taaaacgagc aaaagtcaaa ttgagcaata acccaaccac
    16801 caagatggac atggtttcca cacccagaat cttttgagcg acagaagtga cggcttcatt
    16861 atttggcact acgccagtga tgctaaaacc gtgctcaatg agtttggcaa gagggtctag
    16921 attggtcaca ataacacccg cccctgcagc aagcataagg tatcccaaaa tcggccccaa
    16981 ggttcctgtc aacaccttat gagcaggtgt tttcaaagcc accaaccctg caaaggcaat
    17041 caagcccatt aaaaatgcag gttcttttaa aatatctcga ataaatgata ataatgcttc
    17101 catggtatct tcctatctcc tttaaaggct tattccttgc cactcatttc cttgagaacc
    17161 ggaaccaaat cttcttcaat ttttttacga ttggtatagc ttttaacgat acaaaccttg
    17221 gcatcctctg gaaaaatatc tttaaattct ttaacggtaa caaacaaatc tgcatccttt
    17281 ccaacagccg cattggaatc acaggattct gcatccacat caatacccaa actagatgcg
    17341 atagcttcta ctttcatgcg aagtaacaag ctactaccaa taccgtttcc acaaaccgtt
    17401 acaattttaa tcatgtgatt catctccttt ttcaatcata tggctaatca gactcattat
    17461 ttcctcagta ttcttagcct caattaattg ctcaatatgt tcatcatcat ctaaaatcaa
    17521 cgacagttcc tgcaaagcct tcaaatgtga actagaatcc acagcagaca aaacaaaaat
    17581 caattgcact tgcttgtctg gatcaccttc ttgcttcaaa tcaaagctga caggttcttt
    17641 tagttgtaag agagacatgc ctaactgccg tgctcctttt tcaggcgccg catgaggaac
    17701 agccacctta ggggctaaaa caatataggc cccaagctca ttgaccgaat caatcatgcc
    17761 gtcgatataa gatgtttcaa tcatctggtg ttctagtaaa ggctgagcag ccaaacgaat
    17821 cgcttcctgc cattccgaaa cagcatcagc ctgcctaata aaatctgctg ttaacaagtc
    17881 caatagtcct agacctcctt tttcaatcgt taaatggtta ccaatcaggt aacgggataa
    17941 atcactaaca agtccttctt tattagtaat cgtggtatgt ttatgaataa tactcaacag
    18001 gtcatccaaa gcgaattgct ctgacaaggg aagatgaaag tcatcacaga ccatcttttt
    18061 caacatcatt ttctcaacag gccccatcaa agcttgcgtc acataaacag gtttagcaca
    18121 gtcaaaagct actgttgaaa aaatcaaatc aaaggaagct ggatccaaca actttatttt
    18181 gtccagctgg tgaatcctaa taaactgtag ctgtggaaaa agttccttca aggttgcctc
    18241 caacatcaga gacgaactaa tgccattagg gcaaacagat aaggctacca gctgattgct
    18301 tggcctcttc ttaggggcct gcaaccatct cccaaaatga atggtaaaat aagcgacctc
    18361 atcttcatta actagtttcc ccaattgttt ttctagagga gaaaggctgc gcttgaccaa
    18421 gtaaaagaga gattcataat cttgtttgat ttgtttcttc aaaggattgg tcaagttaat
    18481 gtcgtagtaa agtcggtaat aagctggcaa aaggtgactg tataggttct gacgaagttc
    18541 tggggtatcg gtgatagaca atcccgtgtt aaccgccacc gaattgatga tttcctccat
    18601 aatgtcataa ataggttgct gaaagactaa ctctaagtca ccttggatac aaccaagtaa
    18661 cctagattga accaaatatt tttcttcggt tagactgggg aagttgacca ataactggtc
    18721 caccatcgtt tcaacagctg gcgaagctaa aggaaaagta tccactgctt cagctcttac
    18781 attccgtgaa aatggtcgat gagcgagaag gcagaaaaaa taaatcaaat ccctcaattt
    18841 ctccgaaata aaagttaagt ggttctccct gctcagttcc tccaacgttg ctgccatcac
    18901 ctttgtctgg tcgggcaaac caagttcatg caataagtaa ctgaacaccc aagatcctga
    18961 tatgacttgc aaaagagaac tgaccgctga ttctaagaga cgccgcaagg ctaagggttc
    19021 tccttcaaaa aaataaccat cctggcgatt gtaagctaaa gttacctgaa aggcttgtac
    19081 ccgatcacgc accaagttta aatcagcaat aacagaattt ctgcttaggc gtaacaagct
    19141 ttccaagtgg ttaatggaga taaattcttt agccatcatg atgtaaatga taatcatgtc
    19201 cagacgctca tcttgaaaga gatagtcgtc taaggtaggt agcatgcgat tcaacaagtc
    19261 cttttcagta aggtctggcg gataaaccag ctgcctgtcc agagcaataa ggggtaactg
    19321 atagagtgac aacgtgtcat tgattttagc aatatctgcc agtatatgac gtttgcttgt
    19381 ctttaatatg gattcaaatg cctccagtga atgtccttta tatttagaaa agagttgata
    19441 gtttctgatg agctcgtgag atagcatgtc atctcctcct ataatgtcag tataaaatga
    19501 agcgttttca ttttcaagac aaattgagtc caattgtctc tcgtcatttt taactgcaat
    19561 tggactcaac aggtgatatc attgtaacaa gttcctcaaa aggtggcaaa aaataggcaa
    19621 atctgggaat tgattccctt tttcttcatg atacaacaaa agatacctca tcactgagat
    19681 accttttgtt gctaaataaa atcatcaact gctaaaaaaa cagtcttaga ccattctagc
    19741 caaatgtgta agttaacaaa caacatgtca aaaatgaaaa agcccactgc ataagcagag
    19801 gactttttaa acttgaaatt aacgacgaag tccaagagat tggatcaact cacggtaacg
    19861 gttaacatct gtacgacgta ggtatgccaa caagttacga cggtgaccaa tttttttcat
    19921 caatccacgg taagtagcgt ggtctttttt gtgttctttg atgtggctgt tcaagtggtt
    19981 gatttcccat gtaagaactg ctacttgaac ttcaactgaa cctgtatcgc cttcgtgacg
    20041 tgcatattga gcaatgattt catttttttt ctcttttgag attgccataa tatattctcc
    20101 tttttttgct taatccgagt cctaggattg gcactcctaa aaccaagaaa aagttggttt
    20161 gtctttcgac cactctattc tagcagattc ataatattag gtcaaccgtt atcctattat
    20221 tctgagctct cacatccaaa ccacctcact aggcttaaaa aggttatctg gtcattgaat
    20281 gtgctctttt tgctttaacc aaaccaccat aagcaacaga actagcaagg ccaaatagcc
    20341 aaaaggctgt ttttaaatca gaataaacct gtgcgattgc ttgcctaata agataaccag
    20401 ctaaaagagg taagatggct gatcccagca cttcagctga taagcggtag cctagcatag
    20461 tcattcattt ctttccaata aagtgttcac tgataatatg aattgctcta gtattaacaa
    20521 acccaatccc taatccatga acacagctta ttaaccgatg ctacctgcct ttatgacata
    20581 ggtcaagagt tgacacaaaa tgtttgccaa caaaacatta cctataccgt caaagagcgc
    20641 tttgcttcct atatccttaa agcgcaagcc aaccaagagg ttcatctgaa ctttacatta
    20701 ttagcaaacc gttttggaat aagtgatcgc catcttaagc acgtacttaa gcagctcatt
    20761 tttcagagga ttatcgaacg catctgcttc aaaacctgtc gtattgccga tttagacgct
    20821 ctaactcaat taagtgttaa taactagacc tctgaggtat gcagcccatt aacttataat
    20881 agcaatcact ctgaccacta cctgactttt ctttaaaaaa gaaatgattt gtgatatagt
    20941 aaatccagtt aaaaaaaagt aaaggagtag acatgtctgc acaagataaa ttaattaaac
    21001 ctagtcatct gattaccatg gatgacatta ttcgtgaagg gaatccaact ttaagagctg
    21061 tcgccaaaga agtgagctta cccttgtgtg atgaggatat cctattagga gaaaagatga
    21121 tgcaattttt gaagcattct caagatcctg taatggcgga aaaacttggt cttagggccg
    21181 gtgttggtct agcagctcct cagattgatg tgtcaaaacg tatcatcgct gtgctcgttc
    21241 ctaatcttcc tgataaagaa ggaaatcctc ctaaagaagc ctatagctgg caagaagtcc
    21301 tgtataatcc taaaatcgtt tctcattctg ttcaagatgc agccctttct gatggtgaag
    21361 gttgtttatc tgtcgatcgt gttgttgaag ggtatgtggt tcgccatgct cgtgttacag
    21421 tagactacta tgataaagaa ggacaacagc atcgcatcaa attaaaggga tacaacgcta
    21481 ttgtggttca acatgaaatt gaccatatca atggtgtttt gttctacgat cggatcaatg
    21541 ctaaaaaccc ctttgaaacc aaagaagagc tcttgatttt agactaacaa aaaaggccct
    21601 ttagcctttt tttgttagtc taaatagccg ctaaaagagc ctctgcctga gatagcaagt
    21661 ttgcttttgt ctcttttgaa atctcaagcc ttcctgttcc ccaggcatca ggattcacag
    21721 ttgcttttgt aaactctcct gctactgaag ttcggataaa cggcaatagt gctttaaact
    21781 gatcaaatac ttgatcatgc ccgccatttg caactgaaga gaccgtaacc accttaccgc
    21841 caatagcaga tgggcccgtc ggatcagaca aatcaagagc acgagacaac cagtctagca
    21901 ggtttttaac agaacctgga atagagaagt tgtaaactgg tgtaaaaatc cagatagcat
    21961 ccgctgactg aacagcttga cgagcgtcaa caactggtaa aggtgcatta gcttcgatat
    22021 cttgattcaa aacaggaacg tctttccaat ttaagtaaga tacaactgct tgatgttcca
    22081 gagctttttg tgcttgagcc gctaattgat ggttaaaaga cccttcacga agcgagccaa
    22141 caataaataa aatatgtttc atctctttgt cctctttttt taaagatcgt tttaaagctt
    22201 tctaaaagtg gtatttcatt gatacttctt tttgaaattt atcactatgt agtaattata
    22261 tcttctctaa gaagagctgt aaagagattt gtttataatt gtttgaactt tttgagaagt
    22321 tcaatcagac aaagttgttc tttttcagtc aaaacagcaa aggcttcttc cacccgtgcg
    22381 acgtgtttag gcaaaacagc ttcaatcaag cgagtaccct tatctgttaa ggtaaccaca
    22441 taggctcttt tatccgtttt gtctttgcac ttgctaatcc agccattacg ttccatatta
    22501 tttagtacaa cagtcatatt ccccgaagtc gctaacaaag aatcaatcaa gtgattgatt
    22561 cgcatacagc ctttagtata tagtacttca agcacactga actgcgtggc tgttaaatct
    22621 gcctttttaa aaatatcagc cccaaaagca tctagcgttc gctgagcttt acgaaacacc
    22681 accatggctt tcaaagcggt gttcttatct aaatgactca tttgatttcc tcgctttata
    22741 cggtatgtca caatttagta tcagagagat tagaaagtga tcttgtccct cttcttttat
    22801 gactttgcat atgactacca ttatgataat ctagaaaaag tcatcaaaaa gacttaattg
    22861 attatcttct ggcatatttc ctaaaatacc tatctcatcc attttctcaa cgagcgttga
    22921 cgatgccccg cctcgtttac gcaattccat tttagagagg aattcgcctt cttgacgagc
    22981 tttaacgatt tgcttggcca cgttttcacc cagaccttct aacgctataa atggagggat
    23041 aagggtatct cctttgattt ggaattctat agcatcactt ttgtaaaggt ctaatttgcc
    23101 aaacttaaag ccgcgttcta acatttcgtt gacaatctcc aaggttgtaa agaggtcatt
    23161 ttccacattg gtggcttcat tatttttacg ttttatagta atatcttcca ttcttgcttt
    23221 aacagcatct aagccaccac tcatggtttt taattcaaaa gccttcgcac gaatagagaa
    23281 ataagcacaa taatacataa tggggtggtg caccttgaaa taagccaccc gaagggccat
    23341 caagacatag gccgccgcat gggctttagg gaacatgtac ttgatttttc cacacgattc
    23401 aatgtaccag tcgggcacat tgttttctcg catggcatca atatagccat tacgttcttc
    23461 ctcagaaatt ttcagccaga ggcccttacg cacacgctcc ataatggtaa aggccatttt
    23521 cggttctaag cctgcgtgca tgaggtaaac catgatgtcg tcacgacaac cgataacggt
    23581 ttttagggtc gcaatgcctt ctttaatcaa atcttgtgca ttaccaagcc aaacatcggt
    23641 tccgtgagat agcccagaca actgcaacaa ttctgcaaaa gtggtcggat gcgtttcgtt
    23701 aaccatgccg cgaacaaagt tggtcccaaa ttctggaatg cctaacatac cagtcggtgt
    23761 cccaatttgt tccggggtaa cgcccaaaat ctctgtccca gaaaagagag ccataacacc
    23821 tggatcatca gcagggatag taataggatc aatgcccgat aaatcctgaa gtttacgaat
    23881 catggtcgga tcatcatgcc ctaggatatc aagtttcaag acgttttcat caatatcatg
    23941 gaagttaaag tgagttgtct gccaagaagc cgttacatca tcggctggat attgcacggg
    24001 ggtaaaatca taaacatcca tgtaattagg aacaacaaca atccccccag ggtgctgccc
    24061 agtcgttcgt ttcacaccag cagcacctgc tgctagacga tccacctcag catcccgata
    24121 gaacttgcca tagtcgcgtt catagccttt gacaaatcca taagctgttt tttctgctac
    24181 ggtaccaact gttccagcac gaaaggcgta ttcgtcacca aaaatatctc ggacatccaa
    24241 atgggcactc ggctgatcat caccagagaa gttcaaatca atatcgggca ccttatcccc
    24301 gtcaaatcca agaaaggtct caaagggaat atcttggccg tctttttgat aaggggtgcc
    24361 acattttgga caaggcttat taggtaaatc atagcccgat ccgactgacc catctgtgat
    24421 aaattcagaa tgttggcagg acgggcaaac gtagtgaggc ggcataggat taacctcagt
    24481 aatcccaatc atggttgcca caaaactaga ccctacagat cccctagagc caaccagata
    24541 gcctcgctca tttgaccggt taacaagcat ttgggaagcg agataaatca cagcaaaacc
    24601 gttccccaag atagaggtta gctctttttc aatgcgtaaa tcaataatat ctgggagagg
    24661 attaccataa atttcaaagg ctttttgata ggttaattcg gcaaccgtct cttcggcctt
    24721 gtcaatatac ggggtgtaaa gatcgccctt aaccacttcc acttcctcaa tacggtccgc
    24781 aaaatcctga gtattttgca caaccacttg ataagcgagg tcttttccaa gaaaggcaaa
    24841 ctcatccagc atttcattgg ttgttctaaa gtgcgcttta ggtagaggag caggttgggc
    24901 cccttcccca cggccgattg ttctattaat catggcaccc tgaccgagac tacgcacaat
    24961 aatttcacgg taaatttctt cttcaggctc cagataatgc acatttccag tggcaagcac
    25021 aggtttctta gctcgtttcc ctacttcaat gaggtcacga atcacctgct caatacctgc
    25081 ttggtctttg attaattcac ggacaaccaa tggctggtaa atggctggtg gcatgatttc
    25141 gataaaatca taatacctag ctaaatcaac cgccgcatca attcctttag tcagaacggc
    25201 atcaaaaacc tcgccgtcag aacaagctgt acctagtaac aaaccctctc ggtgagcatc
    25261 taagacggtt cttggaatac gcggcacccc ttcaaaatat ttgatattgg aaaggctgac
    25321 caacttaaac atatttttaa gaccaacctg attttgcaca tagatagtcg catgcttaat
    25381 ccgcgctttt ttgtaagaat cctcagccac caaatctgta ttgagttgca aaagattttt
    25441 gatgccatgc ttttctctgg catctcttag aaaaataaac aaaagacgtc ctgtggcttc
    25501 cgcgtcgtaa ttggccatat gatggtggtc tagactcact tggaaacgct tggtgagcgg
    25561 tcccaaaccg tgacgcttgt actcaggata caagtttcta gcaaattcta aggtatcaat
    25621 cacaggctgt gtgattttgg gcaagtcgtg gcgttcataa ttggcgttca taaagcccac
    25681 gtcaaaactg gcgttgtgag caaccaagat actatctttg caaaagtcct gaaaagcttt
    25741 taggacggta accaatggct tggcgccctg caaatgctta tcggtaatcc ctgtcaattc
    25801 ggtggtaaag gcagaaagag gatgcccagg atcaatgaat tcatcaaact gctctacaat
    25861 atttccttta aacattttgg aagccgcaat ctgaatcagg tcattattca tagcagatag
    25921 acctgtggtt tccacgtcaa agaccacata ggtagcttcg tgcaaatcca tatcaacagg
    25981 atcataagaa ataggcacct tgtcctcaac aatattggct tctaggccaa aaatagcctt
    26041 aattccagct ttgcgagccc tatggtagcc atgaggaaaa ctttgcacat tagcatggtc
    26101 ggtgatagca accgccttgt gtccccactt ggctgccgta tcaatcaagc tttctactgt
    26161 cggtaaggca tccatggtag acatattggt gtgggcatga agttcgaccc gcttttgccc
    26221 ttctggcatc aggtctttgc ggtcatgatg gacaatttct ttgacctgct ggacattcat
    26281 ggtgagactc ttcgtaaaag gattggtctc aatattccct tgtacccgta accaagcccc
    26341 cttagcaatc atatcaaatt tacggagctc ctcatcgtct ttggcccatt tttggagagc
    26401 aaacgaggag gtatagtctg tcattttaaa gttaatgata tggcgacctg ttctagtcgt
    26461 tttacgctcc acgtcaaaaa ccatcccctc aaagacaatc cggttttctt cggtctcaat
    26521 ctctatcatt ggtgtgatgg ctgctttttc aaaccctgcc tgacgcttag ctgctcgttc
    26581 cttgtagtca aacttaggag caggtgtggc ttcctcaact ggtggcatca tggcttcaag
    26641 ggatttttgg gcttccaaat tatcctgcac agcctttttc acaagagcct gacgactgga
    26701 aacaaaatcc ttggtcaaat gctcagtcat ctcctgatct gacaccatat ctatggtcaa
    26761 ggtgccaaag ccaaaggctt ctaattgctt gaccagatta ggcaaatgat tttttctaaa
    26821 atgatcgtta ttcacaaaac ctggcgctgc aataatgagt ttgtcatcct cataagtcac
    26881 tttgagcttt gagaaagaag atttaaaact agcactatta cacggtgcat gctcaaaagc
    26941 ttcttggtaa taagcttgaa gcagatcatc tgaataatcc acctgagcag cttggatatc
    27001 aaaggttacc ttaatgtcag ccgcctcaaa agttcgcgtc aaacgatcat gtaattcacg
    27061 ataagttgca atcggtaaaa ccgccgcaaa ggcaaaatga aattcccaca agcgtgacac
    27121 cgaatgcacc tttacctcga taatatcagc agatgaaaag gcacttgaac gtctcatgtc
    27181 aagtggcatt tctatctggt ccatcaattt agcgaataaa tctgacataa ttttctcctg
    27241 attttactag gctttatttt atcacaattc aagatgattt tcgagtttta gatggaaaga
    27301 aaagcagggc tttcttctga cgttctttgg ctaatttatt ccaagtgata gctcagttga
    27361 ggtcattttt ttgtcaatct tttatcgcca agcgtttttg gtaaagcaaa agccactctg
    27421 taccagacaa gttcacagca aaaagaggcc actctaaaca ggcagcctcc tcatttcttt
    27481 tttagtgttc ttttgttaaa atttcaaggg tttcgatgag gttttctgca ttaacttcaa
    27541 tgctgtcacc tgttgccttg attttaattt ccacgatacc ttcagcggct tttttaccaa
    27601 cagtgacacg aattggcaaa ccaatcaaat cgctatcaga gaatttcgaa ccgacgcgtt
    27661 cattacggtc atctgtcaag acatcatacc ctttagccat caagtcagct tctaacttcg
    27721 ccgtcaagtc ttgggccact tgatctttga cattaactgt aatcaaatgc acgtcaaatg
    27781 gggctaattc tttagggaag ttaatacccc aagcataacg gtaatcgcct tttggtgtct
    27841 tgttcacaaa gagacgggca tgctgttcaa tgacagcaga caaaatgcgg ctaaccccga
    27901 taccataaca acccatcaca attggaactg ttctgccatt ttcatcaaga atcgttgctc
    27961 ccatgctgtc tgaataacga gtgcccaact tgaagatatg accgacttcg ataccacgcg
    28021 caaactggag aacaccatga ccatccggag acatttcccc ttctttgact tcacgaatat
    28081 ctacatattc agcctggaag tcacgtcctg gattgactcc tgtcacatgg aaaccatcct
    28141 tgttagctcc tgcaacggca ttggtaaggt tttgcacttt gcggtcagcc acaatgcgac
    28201 taccttgtgc caagttgaca ggcccaagtg agccaaaacc tgcaccaaag aaagcacggg
    28261 cttcttcttc actagctggc tctaaaaaat cagcagctag atagtttttt aatttaacgg
    28321 tattgatatg gtcatttcca actagcaaag caacaacagg ttcattatct gccacaaaaa
    28381 gcaaggtttt gatggtttgt gtttcatcta ctgaaagaaa agcagccact tcatcaatcg
    28441 ttttgcaatg tggtgtctca acttctgcca aggcatcttc agcagctact tttgaggacg
    28501 gtttatattc gttagttgcc atctcaaggt tggcagcgta gcttgatttt gttgaataag
    28561 caatagtatc ttcacctgaa atcatccaag cagccaattc tgccttaatc tcttctaaga
    28621 cctcttttgg aatatcatcc attgacgcaa tagacttgtc aagaaccacc cagcgatcaa
    28681 ggtccgtacg agctggtgta atggccataa attcttggga atctttcccc cccatggcac
    28741 cgccatctcc aataatccct ttgaaatcta gaccagctct ggtaaaaata gcttcgtaag
    28801 cttgacgata atcttcgtag gtcacatcta aatcttcata gttgtgatgg aaactatagc
    28861 cgtctttcat gataaattca cgggtacgaa gcaagccatt acgcggacgt ttttcatcac
    28921 gatacttaga ctgaatttga taaaggttta agggcaattg cttgtaagat ttgaccgcat
    28981 tacgcaccaa agtcgtaaag gtttcttcgt gagttggacc caagataaag tctgagttat
    29041 cacggttttt aagcttatag aggtcctctc cataggtctc ataacggcct gattcacgcc
    29101 agagatcagc tgtcaaaaga gctggtgcca acatttcaac agcaccgatc ttttcaaact
    29161 cttcacgcat gatggtcttg aatttctcaa tggtacgatt tgccagtggt aaataagcat
    29221 agataccagc agaaacttgg cgcacataac cggcacgcac cataagcgcg tggctgataa
    29281 cctgggcatc acttggcatt tcgcgcaagg ttgggataag cagtttactt tgtttcatag
    29341 attattccgt tttaatacat ccccttatcc tcaaaccctt ctggaaacat caagtaagag
    29401 tgaagcggga ttattcaata atttgaatgt gtattccttt cattttgagt aagacctcaa
    29461 cccttgcaaa tcttagggtt gaggtctttt tcgatcttta acatgataag gtcagcttaa
    29521 gatgacctat cttgattaga aaaagacgcg catgatatca ttccatgtca cagcaatcat
    29581 caatacgacc atgatagcaa ccccagctag ggtgatatag gcctctgttt cttgcttgat
    29641 aggcttgcga cgaatggctt caatgatatt catcaagatt tttcccccat caagtgcagg
    29701 aatcggaatc aggttaaaga tccctaaatt gatcgaaagc attgccataa gagataagac
    29761 tgactctaag ccgttttgag cagcctgatt ggacatgtca tacatggcaa caggtccacc
    29821 taatttattg agactaaagc cagtgatcaa tcctttcaaa gcattcaaaa tagtaaacgc
    29881 cccactccaa gctaattcta gaccgcctaa gagcttatcc ttaaatcctg ttttcagact
    29941 cgccttaact cctatcgtgt atgtctttgt atgcttttgt ggtttcacag ccaccgtttt
    30001 taagcgctga tgtgacttgt aggtgacctt aatggtctgt gacgggccta gatcccgtgt
    30061 tgcaaggtct acagcctcag tgagatcatt ccagctagtc accttataac cattaattgc
    30121 gacaatttgg tcattatctc gaagaccagc cttagctgct gctccatttt cttgaacacg
    30181 gacatgattg ctactaaaat ctggcatccc accttgtaaa aagaccaaga ggataaaaac
    30241 cacaatccct aaaataaaat tattcatggg acctgcaaaa ttggtgatta aacgtccgcc
    30301 aatactagca ttttgatatt gaacatcaag cggagcaatt cttatctcag taccgtcttc
    30361 ttcaacaata gtagcatcgt gagcgacctt atatgtcttg gtttcttcta aaaccaagcc
    30421 agtaatactc agctgatctt ctaagtcata gcctgtaaca tgcattggga gactcgttgg
    30481 gtctaactta ctttgagaca agttgatgcg cttaacaaaa ccttgctcat taagggttaa
    30541 acttgctgga gtacctgttt tgatctcagt tttgtcatca ccccaaccag ccattcgtac
    30601 ataaccgccc aagggtaaca tcctcaaggt ataaagagtt cctccttggt caacatgaga
    30661 aaaaatttta gggcccattc caatggcaaa ttctcttact agaatgcctg attttttagc
    30721 aaaatagaaa tgtccaaatt catggacaat cactaaaata ccaaaaataa taataaaggt
    30781 tattattcct aacatagatt tcctttcagt ctattgcttt tttgagtttt gtcgcgttgt
    30841 tgcgactcca aacattacct cttaagatta aaataaacca aagagatgca tgatgggaaa
    30901 aacaaaaatc atggaatcaa agcggtctaa aataccacca tgtcccggga taagcttacc
    30961 tgaatctttg acaccaaagt gacgtttgag agcactttca accaaatcac caaattgggc
    31021 aaaaatggaa aaaagagcga ccaaaaccaa catggtcaaa aagtgatgtg gcgcataaac
    31081 agacctatca atcaccataa agataaaaga cacaaggact gcacaagcga tccctcctaa
    31141 actaccttca atggttttgt ttggagaaac tgtaggcaat agtttgcgtc tgccaaactg
    31201 acgcccaatc agatatgctc caatatcagt ggcccaaacg ataaataagg ccaaaaagac
    31261 cttgtcaatt cctgacaggc gagcattgat caaatgttga aaaccaatcc caacataaaa
    31321 actagttgca atcggaaagg ccgcatcatc aaatgaataa gctctgctat ttaaaacagt
    31381 acctgccaaa atgaaaaaaa ccattaagct gtaaaaagca acattcgcat caattggtaa
    31441 aaaagtgagg taatggtcca tcggaactgc caatacaaag gcggctaaca tggcaaaaac
    31501 accttcaaaa gaaaagactt ctaatctttt cattttcagt aattctgata ccccaatcat
    31561 ggccaaaacg cctacaaata attggaatgg taggtttcct attatgagaa aaggtaggaa
    31621 aatagctact gctactcccc cccaaacaac acgttctttc ataactatct ccttttagac
    31681 cttgccaaaa cgacgttggc ggcggttata atccgcaatg gcctttagta gctctgcctt
    31741 tttaaaatca ggccacaaga ctggtgtgaa ataaaattca ctgtaagctg attgccaagg
    31801 caagaaattg ctcaaacgca gctcaccact ggtgcgaata atcaaatcgg ggtctcggta
    31861 gagatatggc agatggtctg tcatcaagta attagcaatc aaatcctccg taatatctcc
    31921 tggatttaat ttggcatcta agacatcttg agcaataaag cgaacagcac tggtaatctc
    31981 agcacgtcct ccataattga gagcaaaatt caaaattaaa cccgtattac gttttgtttt
    32041 gtcgatggct gcatttagcg ctgccaaggt atcttcaggt aaacgactag tctctccaat
    32101 catttgaatc tttacattgt tctcatgaag cacaggcaca tatttgtcaa agaaggttac
    32161 tggtaaattc atgataaaag acacctcatc ttggggtctg gaccagtttt ctgtcgaaaa
    32221 ggcatagact gtcagaactt taacccccaa ctcagatgca gtaatcgtta cttcttgtaa
    32281 agcatccatc ccagctttat gcccaaacac tctaggtttt agtctttttt tggcccaacg
    32341 tccattacca tccatgataa ttccaatatg ctttgggata ctccccaaaa ctttttttgt
    32401 tgatttcgct tttaatccaa acatgttttt tccttattga taagcatact tatctcatat
    32461 tttatcatat ttcacgcctt ttggcttgtt ttttatcaat tagatcttat cttcacaaaa
    32521 tgttcaccta tcctctgata gatgtgtcag cttttcatga acctgttagc atcagaataa
    32581 cttcagtcca gggatcaaaa caaaaaagtt tggcatattt catccaaact ttccccgcct
    32641 taatggcttt caatggcact gtcagtgtca gtgctatcta ctgtctcaac agcaggctca
    32701 ttaacaacac tggtttcttc tgtttcaacc agtgtcgttt ctgtggtagc ttttgttaca
    32761 atacgcttaa tagctgatag ctcaaatgtc aaaaagacgc catccacatc taacacaatc
    32821 tttttagctg ttgtatcgac ttcgtcaaca atcgcaaaca tgccaccaat cgttacgact
    32881 tcgtctcctt tttcaatagc gtttaactga ttttggcgtt cttgcgcttg ttttttttgt
    32941 tgacgttgca taaaccaaat caatccaagc atcactacaa acattaaaat tgttggaaat
    33001 cccataatgt ctccttttta ttctataaaa gtcatgtctt actatatcaa aaatcaagcg
    33061 gtatagcaag actagacgtt acctgatttc ttctttaaac gagacgctaa gcgcagtgct
    33121 ttactcacga gtcaaaaaag ctaagatgtc atctggtgtc aaacttgaat cacatacagc
    33181 cctttggtgc tgatcgtaac taaccagcaa agctggaact gtcactaatt ggtagttctc
    33241 acgaaaggca gcaagctcag cagcatcagc agcgttttcg ctatccacaa aatagacgtc
    33301 tttttgatta tctgttgcca cttgtgccaa cttaggagca aagcggcgac aataaggaca
    33361 gctcgaacgt cctaaaaaga caatcatgtc tttgccacta gcaatagctg atgtgacttc
    33421 tgcaacactc gacggtataa aattcgcaac gatttcttca aatgtcatgg atgtctcctt
    33481 ctcttttggt ctcatgatac actaaagcag caattttctc aatcacaagg tcttttcaca
    33541 gctcagagct tttatagccg tcaaaaagaa tccgcaaacg gcaatctttt tgtctctatt
    33601 aacaccaccc taatcttttt ggcgcttcat caacaggcta atagccaata aaagcagagc
    33661 tactccagct gctaataagc cttttgagga ggcttcaccc gtttttggga gcttttggtt
    33721 tgttttagct ctttttggcc tagtcattaa tgatttgtct aggtcttttg agctatctgg
    33781 tgttttggtt tgatgattgt ctgtctgact cttttgctga gagggattag ccgtttttga
    33841 ggatactttg aggaccaggg cagttaggcc atcaatagtc aagccttctt tggtaaactg
    33901 gactccctta ggcttagcaa tggcagtaac accagcttgc tcagcatcag caagcacttg
    33961 ggctcctagc aaatagcggt aggcttgagg taaaactacc ttgcgggtct tgttgtctgc
    34021 attgacaaag acagcatagc gatctccatt tgatgccacc gtttggtaac ccatgatgag
    34081 gtcctcttgt tgaataccat cttgccctgc ttgggtgatc aaggtcacat cccgatctac
    34141 ctcagctttg gttgcttttg taaaggcatc tgttgagcga cgcaacgcaa ttagtccttg
    34201 tgtataggct tttgtttggt tgctaatcgg gtgagctctg gaatctgttg cctttgccca
    34261 gtcaaaatga ttgaccgcat cagacgaatc ataagaatcg tggatgaagt aagggtattg
    34321 cgctacagca tcaatcagag tcgccttatt tggcaccttg tcatcagaca cctttgtctt
    34381 gtagtcggga tttagaagct gcttggttcg tccatattcc tgaccagaat ggataaaggc
    34441 agtcccttga gcagttaaaa tcatggtatt tcctagacga atacgcttgt gaatctcttc
    34501 ttcagccact ttaggatcct tattgatgga tttggcaatg acatcatgta aggtcaggtt
    34561 gtcatgggct gcaatatact gcactacatc tccaggggca tctgcttcaa aattactagg
    34621 ctgtgctttg atcgttttga ataaaccttc tagatttttt gcgccaccag taataaaggc
    34681 tgctgtgcct tcatttggaa aacctgattt gagggtattt ctgatatcat cagagaaaac
    34741 accgaccgta ttggttgctt tcatccaatc ttgatctgcc gcaatttctt ttttcccctc
    34801 atcaccttgg taggtacgcc agccttcgcc aatcataatg gtatttggat taatggcttt
    34861 ggctgcctta aaggcttgct caatagctgc cgcatcatgg tcacccatca tgtcgaaacg
    34921 aaaaccatct accttgaatt cacgagtcag ataagtaatc gaatccacca agatacgacg
    34981 actcatggca tgtgtcgttc ctagacgacc tccgccaaaa ctctctctag ctgtaccatc
    35041 agcattcata aaatgatagt agttgggttc caaatcttca aagagatagg ttctagccgt
    35101 gtggttataa accacatcaa aaataacacc cataccacgt ttgtgaatct cattgacaag
    35161 gtttttaagc tctgcgatac gtaaagcagg gtcgttagga tttgccgaat acatgccaga
    35221 aagggcaaag tagtgttgtg ggtcataacc ccagttgtaa ttattgtctg aagacgtgta
    35281 ggctgttgag cggctcttgt ccagctcatt ggcataaaaa taactcaaaa ccggtagcaa
    35341 ttgaacgtgg gtaaccccca agtctttgag atagtctagc tgttcaacga aagctgaaaa
    35401 agtcccaaaa gggtgtgtta acttgccttc tagagcctta tctgacgtaa aatctcgcac
    35461 atgtgcttca tagataatag cgtcttcacg ctttttaaag ttgttaattt tggcaaaacc
    35521 aaggcctgtt ggtcctagtt tgcttggatc aataaaggca gcttttgctg ttttgatgtc
    35581 atcagtagca gtcgcatcat tccaggcagc gagagatttg gcgtaaggat ccaaaaccat
    35641 gactttttcc tgaccgcgcg tgatttcata aaggtaatag tagcctgtgt aatcactaat
    35701 gcccttgaca ctatcagaag tcagatgagc tctccaaaca cccttgtccg acttggtcaa
    35761 atcagcttga ccaaccaccc ttgtctgatc ttgtttatca taaacgacaa ccttaacagt
    35821 atcagcactt ggagaccata gcgctaaatc aacagaacca tccttagcta gggtagctcc
    35881 aagttcacca tcgtaagcat agagtttatc ttttaattgc caggattggc gagcgacttg
    35941 gctaacttct ccaaatgtaa ccgtgtagac tgcaccttgc gccttaaaat caccctttaa
    36001 tgtcattaca gacttatcct tgtcaagtgt caactcatca attgcaacag ttttccctgc
    36061 tttgtcagtg atcttgatgt tttgtttcac cgcatcttca tcaagcccat ctaaggtcgt
    36121 aaaaatggct ttaatctcat ttggcgtggt ttgttcagct cctttgagac taacctgatc
    36181 aatataataa ggattgttgt aaacttttgg gtcagtgtct ttgacaaaaa cctgagtatg
    36241 attgtctaac tctttaaata gataatcttt tggttgcact ttaatagcat ctcctgtctt
    36301 acttttatca aggattaaaa atccgatttc gttagctcct tcttttaagg ggacatcaac
    36361 ataagctcca taaggccctt tatgtgacaa gtcaagtcca tttggccagt cggtcgttgg
    36421 ggttttgaca tctttaaagg tccagacagc taagttatcg tagtgtcccg attgattgtg
    36481 gtagttgatt cgaaggtaac cttttttcaa aggtcgataa gcgtgtgcat ggtaattctc
    36541 gtctatccaa acttcattca tttttggcgt gagaagcgag atgtgctggt cctttgaaag
    36601 attctctcca gctttattat taatgagata agacacttgc tggcggtgtt tagctgctag
    36661 tggcacatct agataatagc catagtcatc ttttttcgct tttgtcatgg tgatagcacc
    36721 atttggccaa tcctttgaag gttgatccac atctccccac acccaaagtc ccaagcttcc
    36781 tagcgactca ccagctggca atgttttaaa atgcatacgc aaatgatgct cagcaatact
    36841 agcaggatta gctgacgagc tgtctaaaac tggtttagcc tccttgctag acgattgaaa
    36901 accatcatca gatttagcat tgccatctcc ttgttgagct tcatcgcggg ctagtccaac
    36961 aatggtttta gcagcaactt gatgtgttaa gatactaggg gtgcacccta acacgacagt
    37021 cccagttgca gcgattacaa aacctatccc ccactttttt aacagatact gatagcgctt
    37081 tgatccttgg ttgactttct ttttcataag acctccattt agtataacat tcaaacaaga
    37141 ctatcttatc acattgcgca agcgcttgca agacatgccg cacttcaact tagaacacag
    37201 ctcaaataat gactatcacc ccttaatagg atctcaaaaa ttaaacagcc aaaacgagtt
    37261 tccactcgct tggctgttgg gtaaactcaa atcacataca actgctaggc cttcagtttg
    37321 agacaaaagg catcccaagg ttgtaagtgc ttggtctcta ataccgtgtc cacatctgta
    37381 ttggtgatga tcacttgtgc gcctgctaag tctttggcaa agacttgctc ctcatcagac
    37441 acattgacaa caattacata cgtctcttct ccaagctgac gctgataagc aaacacttta
    37501 tcagctgttg gcaagaggtg ataatccgct tcaactagcc agtcctgctc cttacgcaag
    37561 gcaatcaatt tttggtaggt gtaaaaaata gagtctggat cagcaagggc atcggccaca
    37621 ttgatgtctt ggtagttggg gttgaccggc aaccaagtct cttttgcttc agaaaagcca
    37681 gcatgtgtgt ccttagacca ctgcatcggc gttctagcat tgtctcgtcc taccttacgg
    37741 atgctatcca tcacacgcgc agctgacact ccatttccca tagcttcctt agcatagtta
    37801 agggactcaa tatcattaac ctcggttaag tctttaaaag gataattggt catgccaatc
    37861 tcttctcctt ggtaaatgta aggggttcct cgcatcaaat gcagtaaaat agctaaagcc
    37921 ttggctgatt tttctcggta agtgctgtca ttgccccaaa tggataagac ccgtggcaag
    37981 tcatggttat tccaaaaaag cgaattccag ccttctccca actttaattt tgtctgccac
    38041 ttgctaaaaa tagctttcag agcaggcacg tctaattctt tggcataatc ccacttggga
    38101 gcgtcaggtt tatgttgaag acccacatgc tcaaattgga agaccatgga taactcttta
    38161 ttttcagggc gagaatactg gcgagcaatt tcaggcgttg ctccccaagt ctcaccgaca
    38221 gtcatcacgt catgattgcc aaaggttgct tgattcattt ctttgaggta atcgtgtaac
    38281 cgcggcccgt tacctgtgat ttccaagtca ggaaccttcc caatcaggtc aatcacgtcc
    38341 atccgaaaac cacctatgcc tttggcaatc cagaaattca tcatatcgta aattttatga
    38401 cgcaaatctt cattctccca gttgagatct ggctgacgtt tgctaaaaag gtgcaaataa
    38461 tattgacctg atgcattatc atattcccaa gctgagccac taaaaataga agtcaaatcg
    38521 ttaggttcat ctcgccaaat ataaaaatca cgctcaggac tattagggtt ttccctagct
    38581 tcaacaaacc aagcatgctc atcagacgta tggttgacca ccaagtccat gataatctta
    38641 atgcctcgct cattagctgc tgctaagagc tcatccatat ctgccatgtc tccaaaaaca
    38701 tcagcaatcg cttcatagtc agagatgtca tagccattgt catccattgg actttggtaa
    38761 acaggagata accaaatggc tgtaatgcca agtttttgca agtagtccag ttgactggta
    38821 atgcctttta aatcaccaat accattgcca ttggtgtctt taaaggatct cgggtaaatc
    38881 tgataaatcg tagctttatg ccaccagtgt ttcttcatta ttcttctcct attaatcatt
    38941 cattcatctt ttattttaac gttttttctc ttttggtgca atcgtttgcg caaaatgaca
    39001 tgataaaaag gttggaaccc aaccttttta tgaatatcgc ttatacctta acgaatagct
    39061 tgttcggtat ctctgtcaaa gaaatgccct ttagccacat taaaggtcaa gctaacttgc
    39121 tcccctggac tatggaaatc acgcgcatct acacgagaag caaactcagt ttgtccaagc
    39181 ttgacataga gcatcgtctc agagcccagt aactcagaaa ccaaaacttc tgctgtgaca
    39241 gttgcacttg gataagtatc atgcacgatt tgtctgctag aaatgtcttc tggacgaata
    39301 ccaaaagtga cttttttccc cttgtaacca gcagcttcaa gcatttttgc ttgaccttca
    39361 ggaatcgcaa tagcaagacc atcttcactc acaatgcggc catctttaac ttccacttca
    39421 aagaagttca tggcaggact tccgataaag ccggcaacga atttattagc tggaaggtta
    39481 tacaattctt gtggcgagcc gacttgctca attttaccaa tcgttccatt accttgaggg
    39541 tttttagtgg cactcatgat aacgatacgg tctgccaagg tcatggcttc tgtctggtca
    39601 tgggtaacgt aaatagtcgt tgagccaatc ctacgatgaa tctttgcgat ttcagcacgc
    39661 atagagacac gaagcttcgc atccaagttt gacaaaggct catccatcaa gaatacctta
    39721 gcatcacgga caatagcacg tcccatggca acacgttggc gttgtccacc agacaggtca
    39781 gccggtttac gctctagaaa ttcggtcaaa ccaaggatct gcgcagcttc cttgacacga
    39841 cggtcaatat catctttttt gtacttacgt aatttcaaac caaaggccat gttatcatac
    39901 acggtcatgt gtgggtaaag ggcgtagttt tgaaagacca tggcaatatc acggtcttta
    39961 ggagacttgt cattcaccac ttcgccaccg atttttaact ctccttcaga aatgtcctca
    40021 agacctgcaa tcatacgaag agtagtcgat ttaccacatc ctgaaggtcc aacaaagacg
    40081 ataaattctt tatctttgat gtccaagtca aaatcttcaa cggcataatg agtagtattt
    40141 gggtattttt tatagatgtg atttagattt aattcaacca tgtgtaactc ctatttagcc
    40201 agcaaacgtt ctagctgact gcaaagctag aaaccgctga tatcttttat atgatgcttt
    40261 tattataaca aaaatagcgc tttcaaaaca gagacaaagc gaccaaactt ctttgggcaa
    40321 cttgcacaat agcaggacta gtcaagtcgt aaaggcccat aaaacaagaa aaccatgtaa
    40381 acacaggccg gtcacaggag ccaaaacaag ctgaacgatc tatcatcctc cttatcagct
    40441 acagtccagg cagcaaaaaa gaaagccctg tggctttcct aatatttcaa caaaaagaga
    40501 tgagcaaagg ctaaatcatc caattgcttc aagtatagtc ctgattgctg agcaaattta
    40561 tcaagtttgt attgcaagga gttacggtga atatacaagc gctgcgctgt ctgcaccaga
    40621 ttgccgtgtt ctgaccatag agcgtgaacc acatcggcca tatcactttg aggacttagg
    40681 aattggtgaa actgacgcgt caatgccaaa aaagtgtggt gcgacagcaa agaccacagc
    40741 atcagtcctg aaaatgttaa taattttctg cctgattgtt gccttaaata ggccgttaac
    40801 agctgacatt cctcctcaaa acactctctc aatgtacccg cagctacttg ataccaggca
    40861 tttccaacaa acatggttaa ggccaaacca aaatcacttt ctagcgttgg caagagatct
    40921 tcaagccagc gcaagacctt tattgagatt gcttgtcggc ataaaaatgc cgtctgagtt
    40981 gcactgatag gcaaaatgac ctcaagccct gcaatcatct gttggagcaa ctcaactaag
    41041 tcagctggta aagcctgctg atgattgagg taaatgaact gagaagtctc aaacagctga
    41101 gggggattgc cctgatgatc aagcaaatac cgctgccaag gtgaccctag cggctgcaag
    41161 acctcacact gacccagtcc cacctcaagt aataagcgct ctttttcgga taaacatgat
    41221 ttgggaaaat gcacatattg gtccccttct ttgacactca cccactcttt atctcctaac
    41281 ggatgagggc ctacttgcat ctctggaaaa tagtctttta gctccatagc cattcctttc
    41341 atgacggtct ttaaaccatt ataacacatg actctttatc acacagttcg gtttgttatc
    41401 agcacgattt tgtattttct gactttctaa tcattaaagc taaataatgg ttattcattt
    41461 ttagcaagaa cattcaatca aatagctatt tatcggaata ttaatttatg tttatactaa
    41521 aacaggtatt atttctcttt tttcattatc atgacattat cattaagata tcatttaaaa
    41581 aacaattatt aggtttttgt ttgtatctat aaaattatta tgttattcta ataatgggga
    41641 ttgaaactta acttttagga ggtttctatg aaaaattact tatcttttgg gatgtttgca
    41701 ctgctgtttg cattaacatt tggaacagtc aagcctgtcc aagctattgc tggacctgaa
    41761 tggctgctag accgtccatc tgtcaacaac agccaattag ttgttagcgt tgctggtact
    41821 gttgagggga cgaatcaaga aattagcctt aaattttttg aaattgacct aacatcacaa
    41881 cctgctcagg gaggaaagac agagcaagac ttaaatccaa aatcaaaacc acttgctaca
    41941 gataaaggcg caatgtcaca taaacttgaa aaagctgact tattaaaggc tattcaagaa
    42001 caattgatcg ctaacgttca cagtaacgac ggctactttg aggtcattga ttttgcaagc
    42061 gatgcaacta ttactgatcg aaacggcaag gtctactttg ctgaccgaga tgattcggta
    42121 accttgccga cccaacctgt ccaagaattt ttgctaagcg ggcatgtgcg cgttagaccg
    42181 tatcaaccta aagccgttca caactcagct gaacgcgtta acgtcaacta tgaagtgagc
    42241 tttgtctccg aaacaggagg tttagacttt acaccgttgt taagaaacca ataccatttg
    42301 accacactgg cagttggtga ctctctttca tcacaagagt tagcagcgat tgcccaattt
    42361 atcctatcaa aaaaatatcc agattatatc attacaaaac gtgactcctc aatcgtcact
    42421 catgacaatg acattttccg tacgatttta ccaatggatc aagagtttac ttaccatatc
    42481 aaagaccggg aacaagctta tgagatcaat cctaaaacag gtattaaaga aaagatgaac
    42541 aacaccgacc ttatctctga gaaatattac gtccttaaaa aaggggaaaa gccgtatgat
    42601 ccctttgatc gcagtcactt gaaactgttc accatcaatt acgttgatgt caacaccaac
    42661 gaattgctaa aaagcgagca gctcttaaca gctagcgaac gtaacttaga cttcagagat
    42721 ttatacgatc ctcgtgataa ggctaaacta ctctacaaca atctcgatgc ttttggtatt
    42781 atggactata ccttaactgg aaaagtagag gataatcacg atgacaccaa ccgtatcata
    42841 accgtttata taggcaagcg acccgaagga gagaatgcta gctatcattt agcctatgat
    42901 aaagatcgtt ataccgaaga agaacgagaa gtttacagct acttgcgtta tacagggaca
    42961 cctatacctg ataaccctaa cgacaaataa ccacgatctt ctaaaacgat gagattaact
    43021 gacaaaaaaa gcaagcaaca cgctatcaac agttgcttgc ttttttctaa cctcttagtt
    43081 gtagagacta gtgacatttc gtgtctaaaa taatcgtaac tggtccatca ttgacgagac
    43141 taacctgcat atctgcccca aaaacgccac gctcaactgg cacaaactct gccaattgtt
    43201 cattaaagct atcatacaac tgactagcca aatctggctt agcagcacca gtaaaggcag
    43261 gacggtttcc ctttttggtg tcagcataaa gggtaaattg cgacacagat aagatactgc
    43321 ccttgatgtc ttggatagac tgattcatct tgccatcagc atctgaaaaa atgcgcatgt
    43381 tgactatttt tcgcacagcg taagccaaat cttctgcatt atcatctggt cccacaccta
    43441 ctaacaacaa caatccttga ttaatagcgc cagcaatttt cccatcaata gaaacagagg
    43501 cctctttgac ccgttgcaag acaagtttca tggttttatc ctttcttaac cattggtccg
    43561 cttcacgcta taaacatctg gaactgcctt gattttttcg acaacagtgg tcagatgcgt
    43621 cagatttgga atgccaaagc tcacgtgaat attagcaaac ttcatgtctt tggtcggctg
    43681 agcattgact gtcgatatgc tcttggttga gtttgataaa atttggagca catcattaag
    43741 caggccacta cgattgagcc catagatatc aatttcagcc tgataatctt tactcgaatt
    43801 gtccaaatcc cactcgacct caatcaagcg ttcttggtag ccatcttgac tcttaatgtt
    43861 atgacagtcc gatctgtgaa tcgcaatgcc acgccctttg gtaatgtagc cgtcaatagg
    43921 atcaccaggt acaggattac aacacttggc aatccgcatc aagaggcctg atgctccttg
    43981 gataatgact ccattttcac tgcgaacctt gagcacatct ttgttttcgt gtttgacctc
    44041 accgccctta accaattctt cagcttctgc tttagccttg gccctttctt cttcacggcg
    44101 ctctttttcg gttaacttgt taaaaacact gataggacta atgtcaccaa acccaacggc
    44161 tgcatagagc gattcttcac tcttcacact gacttttgga aggatcgctt caatgcgttt
    44221 tttgtcaagg tatttattgg caacgtagcc ctgctcttga aaataagaca ccaacaaatc
    44281 acggccttta ttcactgaca attccttgtc ttgattttta aagaactgac gaattttgtt
    44341 gcgagcctta ttggttttga ccagttttac ccagtctcga ctagggccaa aggaattggc
    44401 attggtgatg atttcaacca catctcctgt ttttaacttg gcagtgagag gaaccatacg
    44461 cccattgact ttggcacctg ttgctttttc accgatttgc gtatggatcg cataagcaaa
    44521 atcaatagga cctgattctt ttggcaactc ctgaacggcc cctgtcggtg taaagacata
    44581 aatccgttca gaaaaaatat cttctttgac cgaatccaca aagtccactg catcgccatt
    44641 tgaggcatct tgcaattcta ccagctcttt gatccagttc atgccaacgg cttgctcagc
    44701 ctgattgacc ttaccacgca cgcctttttt ataagcccag tgtgcagcaa ccccgtactc
    44761 agccacttgg tgcatttcct tagttctgat ttgaatctca ataggtcctt ttggcccata
    44821 cacagtggta tgaatcgact ggtagccatt agctttagga gctgcaatat aatccttgaa
    44881 gcggcctggc atggggcgcc aaagctcgtg aatatagcca accatagcat agacatcgct
    44941 ttgcgtttcc atgacacaac gaatggcaat cagatcaaaa atctgatcga aacgtttttt
    45001 cttgtcccgc attttccgat aaatcgaata aatgtgtttt ggccggccat acacatctcc
    45061 aaacaagcct tgttgtgtcg tataggtttt gaccttactg acaatagcct ctaccaaagc
    45121 ttcacgctcg cgacgttttt ctttcatcat atgggaaatt ttgtaaaatt cggtttcatt
    45181 gaggtaacga aaagccaaat cttctagttc ccatttgatg cgactaattc ccaaacgatg
    45241 cgccaagggg gcatagattt ccatggtttc gcgcgaaatg cgctcttgtt tgtccttgcg
    45301 caaatgtttg agggtgcgca tattatgcag gcggtcagcc aatttcacca aaatcacgcg
    45361 aatatctttg gacatagcca tcagcatttt gcgatggttt tcggcgagtt gctcctcatg
    45421 agatttgtac tcaactttac ctaacttggt gacaccatca acgatatcac gagcatcttg
    45481 gccaaagtct gcttcgatct catctaaggt aatatccgta tcttctacga catcatgtaa
    45541 aaagccacaa gcaactgtca cagcatccag atgcaaatca gccagaatcc ccgccacctg
    45601 aatcggatgg acgatatagg gttcgcctga ctttctcact tggtagaaat gggccgctgt
    45661 tgcatatgct aaagcctttg ccacaaaagc cacatcagcc ttggtcatat aggtggccgc
    45721 taaggcaatg acttcttctc ctgttacgtt cattattttt gccatttcgt tcctcatcta
    45781 gcatttcttt tccctatttt aacactttac acacaaaagc aaaaggcaga atctgctaag
    45841 aaagcgctta gtgtgacctt ttcttagaaa acggttagtg agctgctttt gatatctttt
    45901 gggatctctc aggcgtgagc atgataccaa cggccagctc ccgaccaaaa atagagagaa
    45961 ccaaggggct ctctctatta tgtttgctta tcagtaaggt ctctcaattc agaggattag
    46021 ttgttttctt tgcgttttgt aacggctagg acaccagctg ttgtcattac tgctactgct
    46081 gctgctgtaa agaatgggtt ggcttgctca ccagttgctg gtagacgtct gtggttgtta
    46141 gctacaggtg tcttagccat attgccattg cggcgttctg gcgtttggtt agcttctttg
    46201 tcagcaggt
//
LOCUS       DAUGFP010000011        42791 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.011, whole genome shotgun sequence.
ACCESSION   DAUGFP010000011 DAUGFP010000000
VERSION     DAUGFP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42791
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.011"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(51..1247)
                     /gene="tuf"
                     /locus_tag="VH663_000602"
     CDS             complement(51..1247)
                     /gene="tuf"
                     /locus_tag="VH663_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEQ8173823.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            complement(1601..2905)
                     /locus_tag="VH663_000603"
     CDS             complement(1601..2905)
                     /locus_tag="VH663_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEQ8173824.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILA
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
     gene            complement(3045..5843)
                     /gene="ppc"
                     /locus_tag="VH663_000604"
     CDS             complement(3045..5843)
                     /gene="ppc"
                     /locus_tag="VH663_000604"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEQ8173825.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQTIIAEEVALLKEMLENITRR
                     MIGDDAFTVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINHQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQIQLTNQPAARMNSSVGLNTSAWPSPASLEKPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQEACVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     GNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            6002..7801
                     /gene="pepF"
                     /locus_tag="VH663_000605"
     CDS             6002..7801
                     /gene="pepF"
                     /locus_tag="VH663_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ8173826.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEEALQDIDLFKRN
                     YEDRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMDDFEVDGKTYKNSFVSYENFYQNHENAEIRDKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYTSVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNDLNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVMSTAGYLNLKNNPDGAKEWLDFLKSGGSRTPFDTAMLIG
                     ADIATEKPLRDTIQFLSNTVDQIINYTEEMSHA"
     gene            7794..8273
                     /locus_tag="VH663_000606"
     CDS             7794..8273
                     /locus_tag="VH663_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEQ8173827.1"
                     /translation="MPNLYDFTVKAQNGNDLSLAAYKKKVVLIVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFAWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(8319..8705)
                     /locus_tag="VH663_000607"
     CDS             complement(8319..8705)
                     /locus_tag="VH663_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEQ8173828.1"
                     /translation="MNKRFKTGFLALVAMLLVACSQGTKQIQTTPSVPKADHHVRLVV
                     KEDTNTIDEKVSFGKGDTVLEILKDNYEVKEKDGFITAIDGIEQDTKANKYWLFKVNG
                     KMADKGVDQITVKDGDSIEFYQEVFK"
     gene            complement(8689..9192)
                     /locus_tag="VH663_000608"
     CDS             complement(8689..9192)
                     /locus_tag="VH663_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8173829.1"
                     /translation="MSAKLMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFVLYPLSQQFPKYQLGCQ
                     AFLVALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
     gene            9517..10365
                     /locus_tag="VH663_000609"
     CDS             9517..10365
                     /locus_tag="VH663_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEQ8173830.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKASTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFTTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     regulatory      10492..10588
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10633..11196
                     /gene="thiT"
                     /locus_tag="VH663_000610"
     CDS             10633..11196
                     /gene="thiT"
                     /locus_tag="VH663_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEQ8173831.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMALSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     gene            11287..11766
                     /locus_tag="VH663_000611"
     CDS             11287..11766
                     /locus_tag="VH663_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEQ8173832.1"
                     /translation="MAKKTKLKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            12107..12730
                     /locus_tag="VH663_000612"
     CDS             12107..12730
                     /locus_tag="VH663_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ8173833.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKPDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNTSLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(12838..13740)
                     /locus_tag="VH663_000613"
     CDS             complement(12838..13740)
                     /locus_tag="VH663_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEQ8173834.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DAAIDNFEQLFGAVKVLFDLPDNKFIFDVNDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            13914..15407
                     /gene="lysS"
                     /locus_tag="VH663_000614"
     CDS             13914..15407
                     /gene="lysS"
                     /locus_tag="VH663_000614"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEQ8173835.1"
                     /translation="MSNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKYFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            15543..15683
                     /locus_tag="VH663_000615"
     CDS             15543..15683
                     /locus_tag="VH663_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173836.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(15780..15992)
                     /locus_tag="VH663_000616"
     CDS             complement(15780..15992)
                     /locus_tag="VH663_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEQ8173837.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(16204..17490)
                     /locus_tag="VH663_000617"
     CDS             complement(16204..17490)
                     /locus_tag="VH663_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ8173838.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIVICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            complement(17592..18518)
                     /locus_tag="VH663_000618"
     CDS             complement(17592..18518)
                     /locus_tag="VH663_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ8173839.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            18717..19001
                     /locus_tag="VH663_000619"
     CDS             18717..19001
                     /locus_tag="VH663_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEQ8173840.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLLAKVIKSLLM"
     gene            complement(19161..19589)
                     /locus_tag="VH663_000620"
     CDS             complement(19161..19589)
                     /locus_tag="VH663_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ8173841.1"
                     /translation="MNKSFKNLVIGAVSGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEDDIIIDYTEQDEPVSDTLDKH"
     gene            complement(19582..19989)
                     /locus_tag="VH663_000621"
     CDS             complement(19582..19989)
                     /locus_tag="VH663_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEQ8173842.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            complement(20011..20790)
                     /locus_tag="VH663_000622"
     CDS             complement(20011..20790)
                     /locus_tag="VH663_000622"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEQ8173843.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESLWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            complement(20787..21719)
                     /locus_tag="VH663_000623"
     CDS             complement(20787..21719)
                     /locus_tag="VH663_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEQ8173844.1"
                     /translation="MTVTVKMLVQKVKLDVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            complement(22084..22356)
                     /locus_tag="VH663_000624"
     CDS             complement(22084..22356)
                     /locus_tag="VH663_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEQ8173845.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            complement(22420..22857)
                     /locus_tag="VH663_000625"
     CDS             complement(22420..22857)
                     /locus_tag="VH663_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEQ8173846.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            complement(22844..24976)
                     /locus_tag="VH663_000626"
     CDS             complement(22844..24976)
                     /locus_tag="VH663_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEQ8173847.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            25210..25365
                     /locus_tag="VH663_000627"
     CDS             25210..25365
                     /locus_tag="VH663_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173848.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDKPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            25513..26415
                     /locus_tag="VH663_000628"
     CDS             25513..26415
                     /locus_tag="VH663_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEQ8173849.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKAFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            26415..27230
                     /locus_tag="VH663_000629"
     CDS             26415..27230
                     /locus_tag="VH663_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEQ8173850.1"
                     /translation="MIRFLILAGYFELTMYLQLSGKLDQYINVRYSYLAYISMILSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFVDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLAEVHQTKEPNNPYVYRVF"
     gene            complement(27329..28753)
                     /locus_tag="VH663_000630"
     CDS             complement(27329..28753)
                     /locus_tag="VH663_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ8173851.1"
                     /translation="MGIFPKDFLWGGAVAANQVEGAFEADAKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMGFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     GKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(28772..30634)
                     /locus_tag="VH663_000631"
     CDS             complement(28772..30634)
                     /locus_tag="VH663_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ8173852.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLPMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLALFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFTNALIAGAATIVLAFSLTWFMGIDEE
                     SPKQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GVVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVK
                     RGQALLKMDIDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKMVTKEDALLVIL"
     gene            complement(30636..31478)
                     /locus_tag="VH663_000632"
     CDS             complement(30636..31478)
                     /locus_tag="VH663_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEQ8173853.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLIVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            31806..33173
                     /locus_tag="VH663_000633"
     CDS             31806..33173
                     /locus_tag="VH663_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8173854.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSFVLFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            complement(33227..34777)
                     /gene="ftsY"
                     /locus_tag="VH663_000634"
     CDS             complement(33227..34777)
                     /gene="ftsY"
                     /locus_tag="VH663_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEQ8173855.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQDK
                     IEVVQQEDVSSEGAGSVENGPEAASVNAQVEEETGDNSNHSSEDTNEFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATYDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KEVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(34777..35601)
                     /locus_tag="VH663_000635"
     CDS             complement(34777..35601)
                     /locus_tag="VH663_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8173856.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAAREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLINDESGVAEAVKTFILEEES"
     gene            complement(35605..36402)
                     /locus_tag="VH663_000636"
     CDS             complement(35605..36402)
                     /locus_tag="VH663_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8173857.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYRGEILFADMMTKEQYTEVAKKVLANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            complement(36540..36854)
                     /locus_tag="VH663_000637"
     CDS             complement(36540..36854)
                     /locus_tag="VH663_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173858.1"
                     /translation="MTRLISQQILSEYVKSLEVEADKVVIPGQNTPVFSWDRDGNGNL
                     SIDTPLLKVRGESLATQADLTTISLTPGPKGELWQTSVERLDIKVDKHISLTFKNGVR
                     ID"
     gene            complement(36920..37372)
                     /locus_tag="VH663_000638"
     CDS             complement(36920..37372)
                     /locus_tag="VH663_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ8173859.1"
                     /translation="MGLLDLIGRKRARDKPQNSYEGQDFSYLFGRTISGENVDEFKTM
                     QTTAVYACVRILAEAVASLPIHIYERTENGKEKKLDHPLYFLLHDEPNPEMSSFIFRE
                     TIMSHLLIWGNAYVQIIRDKSGMVSLLLKQKASVLIRKLLLIIWQRMS"
     gene            complement(37477..37697)
                     /locus_tag="VH663_000639"
                     /pseudo
     CDS             complement(37477..37697)
                     /locus_tag="VH663_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37716..38021)
                     /locus_tag="VH663_000640"
     CDS             complement(37716..38021)
                     /locus_tag="VH663_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ8173860.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQGM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(38021..38323)
                     /locus_tag="VH663_000641"
     CDS             complement(38021..38323)
                     /locus_tag="VH663_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEQ8173861.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLITSFEDAKTGNRQDVREFLSQLKENA"
     gene            38369..39463
                     /locus_tag="VH663_000642"
     CDS             38369..39463
                     /locus_tag="VH663_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173862.1"
                     /translation="MTQLKTQYPSLIIDASFITLAANATVKPQDKTYNTVNLLNRFFL
                     IWLDVASRGLSHHHVLSHPFKDWMTTMLDFPFHHEFIEFLGRWTHILITLLKRHHGQA
                     DTAIPRYLLVTIREKTGIVKSTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCD
                     RPLILTSGNLAAENYEVSAIEKDKDAEPLNLIALCPDCFLTYQADSRKKIVTALKNVK
                     KILVSAHNSQQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTL
                     YQLVKNPVIDNYLTIQKIIVNLDKQGRIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTI
                     SEKLHKATLQDIYFCQMIVSYFIQKCEVLE"
     gene            39463..39687
                     /locus_tag="VH663_000643"
     CDS             39463..39687
                     /locus_tag="VH663_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173863.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            39678..41336
                     /locus_tag="VH663_000644"
     CDS             39678..41336
                     /locus_tag="VH663_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEQ8173864.1"
                     /translation="MLIEMWSPVFKNHGEIRESICFHYGLNVIMGMDLADNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPIYFCFQFEKKFYFSRDTANPNIILVCDKSYSP
                     TGETMDLGIFLNRLKKRYHLDSPELSFRLAMSGFFRIAGKSNQNTDFPLQVYSSQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYTFISNLVGGKKQFEANVSE
                     IKRLEYDLAHLQDTHQDKIDSDDIEKNQQKLQLRNTKLELESSLRDKQRRLKLLDISI
                     EFGLYPTESDLTELQQYFPDTNLKKLYEVEAYHKKLETILDSEFSTERESLIAEIDEL
                     ESQLTTLNQELQELGNIPNLSSEYLESYSKLTATINALKEQNEAYLKESGLSKEKSEA
                     DADLKRSTEDILLDLEVKINSKMREFNNILYPDIRKAPQINLNAHNSYSFYTPDDDGT
                     GTKFKGMIVFDLVMLYLTNLPALTHDSLLLNNVSYQATEALLKLYDQSRSLNKQVFLA
                     FDKASSYSPDANQLLSENTVLRLSSNGNELYGISWNKGENSDEI"
     gene            41326..41535
                     /locus_tag="VH663_000645"
     CDS             41326..41535
                     /locus_tag="VH663_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8173865.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIEDIVEIVDNNI"
     gene            complement(41637..42776)
                     /locus_tag="VH663_000646"
     CDS             complement(41637..42776)
                     /locus_tag="VH663_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8173866.1"
                     /translation="MFDFANNTFLAGLNPASLSLVAVYQSLESVIGVLFNLFGGVIAD
                     SFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAIVLTNVILAFMSAFSGPSYKAFTK
                     EIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYKLLGIHGVLLLDGLSFLIAALLIS
                     FILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHKSIFIITVLSALVNFFLAAYNLLL
                     PYSNQMFGEISTGLYGTFLTAEAIGGFIGAILSGFVNKELSSMRLILFLSLSGLMLML
                     APPFYIMFHNAIILALSPALFSLFLSIFNIQFFSLVQKDVDNDFLGRVFGIIFTITIL
                     FMPIGTGFFSVALNPNNSFNLFIIGSCITILSLVFRILFKKYNIR"
BASE COUNT        13018 a         9050 c         7650 g        13073 t
ORIGIN      
        1 caaacttaag ttgtctgact taaattgcta ttctgggaac ttaattaaaa ttaagcttcg
       61 atttctgaaa cgatacctga accaacagta cgtccacctt cacgaattga gaaagtagta
      121 ccttgttcta cggcgattgg gtggatcaac tcaacgttga ttgttacgtt atcaccaggc
      181 ataaccattt ctgtacctgc tggaagttcg attgaacctg ttacgtcagt tgtacggaag
      241 tagaattgtg gacggtagtt gttgaagaat ggagtgtgac gtccaccttc gtctttagaa
      301 aggatatata cttcaccttt gaatttagtg tgtgggttga ttgaacctgg tttagcaata
      361 acttgaccac gttcgatttc gtcacgttga acaccacgaa gaaggatacc tacgttgtct
      421 cctgcaagac cttcgtcaag ttgtttacgg aacatttcaa caccagtaac aacagctttt
      481 ttagtttctt ctttgatacc aacgatttcg atttcgtcgt tgacacgaac agtaccacgg
      541 tcgatacgtc ctgaagcaac tgtaccacga cctgtaattg agaatacatc ttcgactgga
      601 agaagcaatg gtttgtcagt gtcgcgttct ggttctggaa tgtatgaatc aacagtatcc
      661 atcaattcca tgatgatgtc ttcaaattta gtgtcgcctt caagagcttt aagagctgaa
      721 ccttggataa cgggaaggtc atcacctggg aaatcgtatt ctgaaagaag gtcacgaatt
      781 tccatctcaa ctaattcaag caactcttcg tcatcaacaa ggtcaacttt gttcatgaac
      841 acgataaggt gtttaacacc aacctgacgt gaaagaagga tgtgctcacg cgtttgtggc
      901 attggtccat ctgttgaagc aactacaagg atagctccgt ccatttgagc ggcaccagtg
      961 atcatgtttt taacgtagtc cgcgtgtcct ggagcgtcga tgtgcgcata gtgacgagtt
     1021 gcagtttcgt actcaacgtg tgcagtgttg atagtgattc cgcgttcgcg ttcttctgga
     1081 gcagcatcga tagaagcgta atcttttggt tggttaactg atgaaggcaa gcgacgtgcc
     1141 aatacagttg tgattgcagc tgttaaagta gttttaccat ggtcaacgtg tccgattgta
     1201 ccaatgttaa cgtggggttt actacgatcg tatttttctt ttgccatttt aggaaaagcc
     1261 tccaataaaa tatattttat agatagacgg taggccatac agtctaactt taccttacta
     1321 ttgtagcaaa ttacaatgaa aatgcaagta ttttatgttt tttgccattt ctttattttt
     1381 taaatagaag aaaaagtctt ttttgatact ctccttaggt ggtgcttata gcagcgactt
     1441 cctttggaat tccacacctt atttttgcca cagcaaaaag cagctgtttg ggctcatgtc
     1501 acacgcaaaa ataagaaatc agcagagcag ttcaagtttt ttgagcatca aactgggatt
     1561 gtctatattc taaaatctcc ttaaaggaga ttttagaata ttattgaaat tgcttgatat
     1621 ttacaacttt actattttct tttcgagtgt cctttcgata agataattca gcttctttaa
     1681 atatatcatc tcgcttttcg ctagcatcaa tatttagtac aaaacccact gcaacagata
     1741 agacaagtaa gctatttcca ccttgagaca aaaaagggaa ggtaacacca gtagatggaa
     1801 ttaagcctga gataccacca atattaacaa agacttgcat gagcatcata ccgcctactc
     1861 ctaaagccat catggcatta aaaggatttt ttgctttaat accaacattc ataatacgta
     1921 aaattaagaa gaaaacaagt gccaaaataa accctgctcc tattagacct aactcttcaa
     1981 tgacaactga aaagacaaag tctgtctgtg cttcgggcaa atagccacgc ttttcaatgg
     2041 aatttcctaa cccttggcca aaccacccac cattactcat agcatagtaa gaattggcta
     2101 attggtgacc tgaatcggtc aagtcatgaa aaggattaaa aaaggcacta aaacgtttag
     2161 cgacatagcc aaaaacagga atcttagcaa ctctttctac cccaataacg gcaatagtcc
     2221 caagaaagac cgtggataga ccggtaatca tggctaggat agccgaaaac catcggtaac
     2281 caatgccact gatagaaaac ataataatgg ctgtcaatac aataatggaa gcatttccta
     2341 agtcaggctg agcagcaacc aaaagtacca tcaacaaaga ataaacacgc cagtcccgta
     2401 aatcgttcca ttgtgttggc caccattttc gacgagttag tgcttgataa tcataaaggc
     2461 taatgttttt ttggatctta gcaaaggtca gtgccaaata ccataccata atgatcttta
     2521 aatactcagc aggctgaaag ctgactggtc cgatcacaat ccaaccatgc gctcctttga
     2581 tagcagttgt aaagaagcgg gcaataatta aaagaaaggc ctctcctaac ataaccacag
     2641 ttaataccct tgtattcgtt aaaaaattta attttaattt atagataaat gtaatagcca
     2701 ctagacttat tatccaaaaa actccctggt taatcaccga tttaaatgga ttagcatgtg
     2761 cttgaatcaa gctgacactt gtggttgagt aaaccattat taagcctatc accgataaaa
     2821 ttaaataagg caataatata gaataattta agaggtgcct tttatcaatc ttcatagttt
     2881 tccctctact tcttgagata atcatttagc attataacat ctttctatat caaacaaaag
     2941 gaaaagtcaa gaatcctcaa aaaaagaagc tccaagggct aacctttaaa attctgatta
     3001 gtcacttgaa aacttctata aataccaatt aatactcaag agtattagcc tgaattacgt
     3061 aaaccagttg ctatgccatt gatagttgta tggatcaatt tttcatcatt ttcgtcaagt
     3121 tgattattac gccaacgttt aatcaattca atttgaatat agttcaacac attaaaatac
     3181 ggcaagcgtg acttcaagct atgcttaagg gaaggattat cctccaatag atcatcatgg
     3241 tcttggatag caagaataac attttttgtc aattgccact catccaaaat ctcataaaaa
     3301 acatctcgaa catcctgact ttcggctagt tgtgcatact gaaaggcaat attcatgttt
     3361 gatttagata ataccatatc aacatttgat aacaaggaat ggaaaaaagg ccacgtctga
     3421 tacatgtgtt ggaggcgttc aaggttacct tgtgctcggt caatataacg tttaaaggct
     3481 gaacctaccc cataccaacc tgggaacatg atgcgatttt gtgaccatga aaagacccaa
     3541 ggaatggcac gcaagcctgt aatttccgta atcgttttac gggctgctgg tcttgaccca
     3601 atgttgagac tggaaacttc cttaatcggg cttgcctcaa agaaataatc atagaaatgg
     3661 ggattgtcaa aaaccaattt tcgataaatg gtgttgctat cagcaacaat gctatccatg
     3721 atttctcgaa aactatcaat ctcattagga tctgttatca tttgagtcac catacgattg
     3781 atagaagctg aaatcaacat ttctaaatgg taataagcca catctttatt gccgtatttg
     3841 ttttcaataa tttccccttg ctctgtcaga cgaatccgat ctttgatgga accaaaaggt
     3901 tgtgaagtaa tggcatcata agaagggcca cctcctcgac caactgttcc tccacgacca
     3961 tggaagaagg tgattttcac tccatgttcc tcaccaatag ccgttaattc attctgagct
     4021 ttataaagag tccaacccga tgccaagtag ccaccatctt tattactgtc agaataacct
     4081 aacatgattt cttggtaatt acgattcgtt gcaatccaag atttgacaat gtcatggctt
     4141 aaataggctg ccatgatgtc acgggcatta tctaaatcct caatggtttc aaagagggga
     4201 acaatttgca cacgagcccg ctgctggtca accaatccca cttctttaag catgatggcc
     4261 aactcaaaca tatcagagac actttctgta tgtgaaataa tatgttgctt aataacctct
     4321 tctccaagct tatctttcaa ttctcgtgcc gtagtataga tagccagttc tttttctaat
     4381 aggtctgatt tagcaactgc cgctgaggat aaggttcttg gctcctccat taattgttgt
     4441 aagagaacat cgcatttttc agtttctgaa agagagctat aatcgtcaac aatattggct
     4501 ccttttaata actctgccac acaagcttct tggacactgg agtcttgcct catgtcaatg
     4561 cttgctaaga agaagccaaa aatatcaaca gcttgcatga cttctcgcaa atccccctca
     4621 atcaaagcgg agttgccatt atcaagtagc gactgctcaa tagcctttaa atcagcttta
     4681 aaatcaacag gagagtcata ggctaagatg ggtttttcta aactagctgg tgagggccaa
     4741 gcagatgtgt ttagtcccac actactattc atacgagctg ctggctgatt agttagttga
     4801 atttgtgttt gtttaagacg cgattgaata taatgaaaag ctctgcgata aggttcattt
     4861 ccacggtaaa tcgattggtc ctgagacaaa ctggctaaac gttccacttc cgagttgggt
     4921 tgtaccaagg ttgacgacag tgaaaatgtt cggtaaaggg ctgctaattt gtcaatgtag
     4981 taattgagga taacctcact ttgaaccgta gctgacaagc acaaggtttc agctgtgaca
     5041 aaggggttgc catcacggtc cccaccaatc cacatcccca tggtaatggg tttgggattg
     5101 tctaactcta gaccgtgttt ttgggctaag tttttgtact ctgttgtcaa cttggtcact
     5161 gcttgaatga gagaaccatc gtaatactgc atgacatttt ttatctcgtt tttcacctga
     5221 agctttttct cgcgaataat atctgtctgc atgatcattt caatataacg atatagctcc
     5281 tgacgccatt tctcaagatt aatcacacca gccttagcat cacggtattt gcgcagaagt
     5341 ttatgaatgt gagttgtcaa ttccagaatc gttttacgtt gtacttgcgt cggatgtgct
     5401 gttaaaacag gaaccacatt aacctgctcc aaaatgtctt taccattatc ttttccagca
     5461 agatccttga tggttagggc taattttcct aaataatcag tatccgtatt attttgatga
     5521 ttaatttcat aggctaaatc gacatcttct gaaatattga ttagcaaggg taaaatagaa
     5581 aaatacctgg aaatcacttc catttcttga tttgaaatat tggcgacaac tttttctaat
     5641 tctatgtaat cttgtttttc agataacacc ataatagact cgataactgt aaacgcatca
     5701 tctccaatca tccgtcgtgt gatattttcc aacatttctt tcaaaagagc aacttcttca
     5761 gcaatgatag tttgattgtt actactctct aactttttaa gtggcaaatg accatctcct
     5821 tctttgctag tttcttctat cataccactt tttaccaaaa ataaaagtga gcatgttcat
     5881 aacgtttgtt ttttaacaaa agtaagcgct ttatttcaaa aaatgtttgg ataggtttag
     5941 tctccatatt ccactaaaat tctgttatac tagtcttgac tatattgtta aggagacaac
     6001 tatggaattg aaaaaacgtt cagaattccc tgaaaatgaa ctttgggatc taacagcgct
     6061 ctacaaagac cgccaagatt ttttactcgc tattgaagaa gccttacaag atattgacct
     6121 gtttaagcgc aattatgaag accgcttaac atcggtggac gatttcactc aggccttaat
     6181 agaaattgag catatttata ttcaaatgag tcatattggc acttatgcct ttatgcctca
     6241 aaccactgac ttttcagatg aaagttttgc tcaaattgct caggctggcg atgatttcat
     6301 gactaaagct agcgttgccc ttagcttctt tgacaccgcc ttagccaatg ccgaccttga
     6361 cgtgttagat acattagaaa agaaccctta tttcagcgca gctattcgca tggcaaaaat
     6421 ccaaaaagaa cacctcttaa gtcctgatgt ggaaaaggct ttggctaact tacgcgaggt
     6481 tatcaatgcg ccctatgata tttacaccaa aatgcgggct ggtgactttg acatggatga
     6541 ttttgaggtg gatggtaaaa cctataaaaa tagctttgtt tcttacgaaa atttctatca
     6601 aaatcacgaa aatgctgaaa ttcgtgacaa agctttccgt tccttctcaa aaggtctccg
     6661 taaacaccaa aatactgctg ctgctgctta tttggccaag gttaaatctg aaaaattgtt
     6721 agctgatatg aaaggctaca cttctgtctt tgattacctc ctagctgaac aggaagtgga
     6781 tcgtagcttg ttcgaccgcc aaattgacct catcatgact gaatttggac cggttgctca
     6841 gaaattctta aaacatgtcg cccaagtcaa tggtcttgag aaaatgactt ttgctgactg
     6901 gaagctagat attgacaatg atctcaatcc agaagtcagc attgatggtg cctatgacct
     6961 cgtgatgaag tctttagccc cattaggaca agaatacacg aaagaaatcg aacgttatca
     7021 gacagaacgc tgggttgact ttgctgctaa tgccaataaa gattctggtg gttatgccgc
     7081 tgatccttac aaggtccatc cgtatgtctt aatgagctgg actggcagaa tgtctgatgt
     7141 ctacacgctc attcatgaaa tcggacactc tggccaattt attttctctg ataatcacca
     7201 aagttacttc aacacccata tgtcaactta ttacgtcgaa gcgccatcga cctttaatga
     7261 attgatgctt agtgattact tggaacatca gtttgatgac cctcgtcaaa aacgttttgc
     7321 tcttgctcac cgtttaacag acacctactt tcataacttc atcactcacc tattggaagc
     7381 ggctttccaa cgtaaggttt acaccctgat tgaagaaggc ggtacctttg gagctgacca
     7441 attaaatgca atgatgaaag aggtcttaac agacttctgg ggagatgccg ttgacattga
     7501 cgatgacgca gctcttacct ggatgcgcca agcgcattac tacatgggct tatatagtta
     7561 tacctattct gctgggcttg ttatgtcaac agctggttac cttaacctta aaaacaaccc
     7621 agatggggca aaagaatggc ttgacttcct aaaatcaggt ggcagccgta cgccttttga
     7681 tactgcaatg ctcattgggg cagacattgc caccgaaaag cctcttcgag ataccattca
     7741 attcttgagt aataccgttg accaaattat caattacacc gaggagatgt cacatgccta
     7801 atctctatga ttttacggtt aaggctcaaa acgggaatga cctttcctta gctgcttata
     7861 aaaaaaaggt tgttttgatt gttaacacag ctaccaagtg tggattaacc ccacaatacc
     7921 aggccctgca agcactatat gacacatacc atgacaaggg atttgaggtc ttagattttc
     7981 cttgcaacca atttttaaat caagctccag gagatgctga ggagattaat cacttttgca
     8041 gtctgaccta tcacactact ttccctcgct ttgctaaaat caaagtgaat ggtaaagacg
     8101 ctgatccttt gtttgcctgg ttaaaagaag aaaaaagtgg tccccttggg aaacgcatcg
     8161 aatggaattt cactaaattt ctcattgacc aaaatggtca agtcattaaa cgctattcct
     8221 caaagacaga tcctaaacta attgaagaag accttaaagc acttcttggt taatataaaa
     8281 tagacttggc gtgccaagtc tattttatat tatagttact atttaaaaac ttcttgataa
     8341 aattcaatgc tatcaccatc tttaacggta atttgatcga cacccttatc tgccatcttt
     8401 ccattgacct taaaaagcca gtatttgtta gctttcgtgt cttgctcgat accatcaatt
     8461 gctgtgataa aaccatcttt ttctttaacc tcataattat ctttgagaat ctcaagcacc
     8521 gtatctcctt ttccaaaaga aactttttca tcaatggtat tggtatcttc tttgacaact
     8581 aagcgaacgt gatggtctgc ttttggaacg cttggagtcg tctgtatttg ttttgttcct
     8641 tgcgagcaag caacaagtag catagcaaca agtgctagaa atcctgtttt aaatcttttg
     8701 ttcaccgatt aacctcctaa ataacatcat cacaactgga aaaaagacga gagttgataa
     8761 agcatgcgct atattaaagg gcatccctgc taaaacatag ctccaccaag gcatactata
     8821 taaatacgca aaacaagtat ctatcagcac cccataaagg aggccacata aggcgaccaa
     8881 gaaagcctga cagccaagct gatatttggg gaattgctga gacaaagggt acaagacaaa
     8941 acgccataac aaaagaacaa gcacaaaaca agtaacttgc caaaagaccc aagggccaaa
     9001 tcccagcaaa aaggctgata aaaacaagca caacatcata actaatacag cctcggctaa
     9061 cccaaagtaa agaagataac tgagtaaaaa tgccgtcaca ggctgcacgt taggcaggct
     9121 gctaaaaacc attctcaaca caacacatag ggctgacatg atagcaatgc gactcataag
     9181 cttagctgac ataaacttct tcctcgtttt tttctctatt atgacagatt tacaaagcgt
     9241 ttaccagttt ggagctcact tatttatcaa atgataataa catgagaatt aaataacaaa
     9301 acagtacttg ctgactgtca tgatatagtc aaagactaaa ctaatattgt aaagaatata
     9361 gctaccaact ataagagata gtgacataaa cttgacatag ttagagagga tctcatttgt
     9421 aacttctcaa agagaatacc ttaaaatgca agacaatata agaaaacagt taggatattt
     9481 tgattttttt tagtaattag ggtacaataa cagttaatga gaagaaaaat aaaacctatt
     9541 gttgtgctag tcttctttat tttactggca atggtattga ttatcggaaa aagacaagct
     9601 aatcatgcga aacaaaaaga agttgaagac gctaagagtc atattcctat tgcaaccagt
     9661 aatcctagta aagcaaaagc tagcacttct gaaaccgaag actttatttt aaatcctatt
     9721 gttgatgttt ccggctggca actccctgaa gaaattgatt atgacacatt gtcacgtcac
     9781 atctctggcg caattgtacg tgtctatgga ggatcgcaaa ttacagctca taacaacgct
     9841 gcttttacaa ctggaattga caaatctttt acaacgcata tcaaagaatt tcaaaaacga
     9901 aatgttcctg tagctgttta cagttacgca cttggtcgta gtactaaaga gatgaaagaa
     9961 gaggcccgag ctttctacaa aaacgctgct ccctataatc caacttacta ctggattgat
    10021 gtcgaagaag ccactatgaa agatatgaat aaaggcgtaa cagccttccg agaagagctt
    10081 aaaaaacttg gtgctgaaaa tgttggtctc tatattggaa cttattttat ggcagaacaa
    10141 gatatttcaa caaaaggttt cgatgctgta tggattccga cttatggtag tgattctggc
    10201 tattatgagg ctgctcctaa tacaacctta gattacgatt tacaccaata tacctcacaa
    10261 ggttatctca gtggttttaa caatgcttta gatttaaatc aaattgccgt aacaaaagac
    10321 actaaaaaaa cgtttgagaa gttatttggc aactccaaca attaagaggt tagcatcatg
    10381 gctaatcttt tttgtagttt acatcttacc aagaaaaact ctaggtcaat aatttaaccc
    10441 cccttatcct tgacaaggag attatctcat catataatga aagcactata atatttcaca
    10501 aaggagtgct ttggctgaga tcgcaattgc gaaatcctga ggacctgatc ttgttagtac
    10561 aagcgtaggg attgtgacca ataatcaatg attggaaagc gatactcttt ttgtgcacaa
    10621 aaggagtttt ttatgtcacc aaacaccaat gtcaaatacc ttatcgaagc tgctattttt
    10681 gctgcattgg cgatggccct ttcatttatt ccagattttg ctggctggtt tagtccttct
    10741 tatggtgcta ttgcactggt tattttctct ttgcgccgtg ggttaaagta cggcatgttg
    10801 gcgggtctca tttgggggct gcttcacttt gtacttggaa aagtttacta tctcagcatg
    10861 tcccaagtct ttattgaata tatcttagcc tttacctcta tgggattagc cggtagtttc
    10921 tctgatagtt tgattaaaac tttaagaaga caacaaacct tttttgccgt ttttcttgca
    10981 ataatggcta gtttactagc cgttacagta cgttacttgt ggcacttcct tgcaggaatc
    11041 attttctggg gcagttacgc ccctaaaggc atgtccgctg tctggtattc ttttagcgtt
    11101 aatggtacag ctggtgtcct aactttttta ataacatgtt tagcgttaat gattgcttta
    11161 ccaattcatc ctcaattatt tgatcccaaa gactaatttt tagtcatttg aatcttacat
    11221 taaaaaaatg gtataataat gttgttatac cattttttta tgtttagata aaggtaagga
    11281 cttgatatgg ctaaaaaaac aaaactcaaa aaaaccttag tagaacaaat cctcgataag
    11341 gctaatattg ctcatcaagg actcaagctt aatgctttag aaggcgactt tccagatgac
    11401 cttcaaccat cagatattta caagacactt gctctgactg gtgatcaaac aggtcctcta
    11461 attggcatta ttccattaac agaacactta tcagaaaaac aattggcaaa agtatcaggt
    11521 aacaaaaaag tgtccatggt tcctcaaaaa gacttgcaaa agacaacagg ctatattcac
    11581 ggtgccaata atcctgttgg gattcgtcaa aaacattcat atcctatttt tattgaccaa
    11641 actgcactgg aaaaaggtca aataatcgtt tcagctggtg aagttgggcg ttctataaag
    11701 atttctagcc aggctctagc tgatttcgtt ggcgcaagct ttgctgattt aaagaagaga
    11761 aaatgaacac tactgccaac tttatctggt ttctttttta agtttttcgc cttgattaag
    11821 ctagtctttg tttagtatta ttttgatctc aactgcttta acattgatcc tgttaatctt
    11881 tttcttgtgt aaaggcttga tatcacgaca aattcaggtc gatttgtctt aaatctaata
    11941 tgggctttac actttaatca gaacctagct attatacact tatactacgc ttttttactt
    12001 tatcttttca ctataggtat tatttttgct tggtgatgcc taaacttttt ctggttactt
    12061 ttttattttt agcgctcgca tcagttattt ctaaaaggag gcttaaatga aattatattt
    12121 tgtaagacat gggaaaaccc tttggaattt agaaggtcgc tttcaaggcg ctgggggcga
    12181 ttcgccatta cttgaagagg caaaagacga aattcactta cttggcaaag agttagctaa
    12241 ggtggctttt gatgccgttt atactagcga ccttcaaaga gctatggcaa cagcagctat
    12301 tatcttagat gcttttgacc aacagccaaa actttaccac accgaccagc ttcgggagtg
    12361 gagattaggt aaattggaag gggctaaaat agcaacaatg gcagctatat atccccaaca
    12421 aatgctggct tttcgggaga atctagccca gttcaaacca gatcagttcg aggctgaatc
    12481 aatttaccaa acgacccaac gtgtctgcca tcttatccaa tcgtttaaag ataaacatta
    12541 tcaaaacgtt cttattgtag gacacggcgc taacttaaca gcaactattc gctcattatt
    12601 aggttttgaa cctgctcttt tactcgctaa aggtggctta gataatacca gtcttactat
    12661 tcttgaaact aaggattatt taacttatga ctgtttaatt tggaatgata aatcttttct
    12721 agaggtgtaa aaccactaga caataataaa aaaagctaac acattgatct cttactgaca
    12781 agacactcac tttgctagct ttttttatta ttatctagtg ggctattatg gtttcatcta
    12841 tctggcatat tttaagcgca aaactttttc ttgctgcttg gttaatcgaa gcaagataac
    12901 cactttgtca atgctcatgt tgctttcaag taaacgctct gctgccatca tcaagccatt
    12961 attgattccc atctgtaaaa ttggattttt cttgtcattg acatcaaaaa taaatttatt
    13021 atcaggtaaa tcaaagagta ctttaacggc accaaagagc tgttcaaagt tatcaatagc
    13081 cgcatcataa acaggcttag ttcctggttt tagctgacga cctctatgat taaaccacat
    13141 agaatgccaa ccaccattaa aggcacccat gatatcattg tcataagagt ccccaacata
    13201 tagtgtggtt tgtgggttca tgtcaaattg ttctgctgcc aggttaaaaa tttctttttc
    13261 tggtttttga aagcctgttg cttggctcac aatgacacgc ttaggatcaa tataatcata
    13321 aagccctaat ttacgaactt ttttcagctg atgttctgtg ggcccattgg taataatccc
    13381 cataggaaca ttctttgatt ttaaaaagtc aagggtcata cgcatctcat ctaacatggt
    13441 aatgttctca agttcgtgct catagacttc ttgaaaatgt actcccgcag cttcgtcgat
    13501 ttctcgatag ccaaactcaa gcagtgtttc cttgcaacgc caaaatcgga aatattctgt
    13561 ggtccactcc cctgccatta ctcttgggaa tccaacatct gaatagtgac gaaagcgaat
    13621 ataagcttga ttcattactg acatatcaaa atctgggaag cacttttcca tggcgattcg
    13681 ataaggcgct tgctggtcgt aaattgtgtc atccacatca aaaacaatag cagtaatcat
    13741 gagtctcttt tcttttctag tttaatatcc caatcattat actattttcc attaggcttt
    13801 tcaattttat tttcaaaaaa gtgccccgct gcagtcgcat tttccaacga aatataggtg
    13861 tattctagcc tcttttatga tacaatggaa aggaaatcgt attggaggaa aatatgtcta
    13921 atcaacacat cgaagaatta aatgaccaac agattgtccg tcgtgaaaag atgatggctc
    13981 tggctgaaca aggaattgat cctttcggta aacgctttga ccgcacagcc aattctgctg
    14041 aattaaaaga aaagtatgct gataaaacaa aagaagaatt acatgagtta aacgaaacag
    14101 ctattgttgc tggccgcttg atgacaaaac gtggcaaagg taaggttggt tttgctcatc
    14161 ttcaagaccg tgaaggccaa atccaacttt atgttcgcaa ggactctgtt ggcgaagaca
    14221 actatgaaat cttcaaaaaa gccgatttag gcgactttat cggcgttgaa ggcgaagtca
    14281 tgcgtacaga catgggcgag ttgtcaatca aggcaaccaa gttgactcac ctttctaaat
    14341 cacttcgtcc tcttcctgaa aaattccacg gattaactga tattgaaacc atttaccgta
    14401 agcgtcacct tgatttaatt tcaaatcgtg agagctttga ccgcttcgtg acccgttcca
    14461 aaatgatttc agaaatccgt cgttatttag acggtcttga tttcctagaa gtagaaacgc
    14521 ctgtgcttca taacgaggct ggtggtgctg ctgctcgtcc tttcgttact caccataatg
    14581 ctcaaaacat tgatatggtg cttcgtatcg caacagaact tcatttgaaa cgtttgattg
    14641 ttggtggtat ggaacgtgtc tatgaaattg gtcgtatctt ccgtaacgag ggaatggatg
    14701 ctactcataa tcctgagttc acttctattg aggtttacca agcctatgca gactatcttg
    14761 atatcatgaa cctgacagaa ggcattatcc aacatgctgc taaagctgtt aaaggtgatg
    14821 ggcctattga ttatcaagga actgaaatcc gtatcaatga acctttcaaa cgtgtccaca
    14881 tggtagatgc tatcaaggaa gtgacaggtg ttgatttctg gcctgaaatg actgtcgaag
    14941 aagctattgc acttgccaaa gaaaaacagg tcccactcga aaaatacttc acaagtgtcg
    15001 gacatatcat caatgctttc ttcgaagaat tcgttgaaga aaccttggtc caaccaacct
    15061 ttgtctttgg tcatcctgtt gaagtatcac ctttggctaa gaagaatcca gaagataccc
    15121 gcttcaccga ccgcttcgaa cttttcatca tgaccaaaga atacgccaat gctttcacag
    15181 agttaaatga tcccattgac caattatctc gttttgaagc acaagcacaa gctaaagaac
    15241 ttggtgatga cgaagcaact ggcattgact acgacttcgt tgaagctctt gaatatggta
    15301 tgccgccaac cggtggactc ggaatcggga ttgatcgtct ttgcatgtta ttaacaaaca
    15361 caacaacaat cagagatgtg cttttattcc caacaatgaa accttaactt ttttaagatt
    15421 tgtattcata tcttaaaacc gataagtggc aatataacat aacgttttat tatctctaac
    15481 cagtcttcat taccatgaag actggtttat taacagtagc ttatagaaag tgacttaaca
    15541 agatgaccaa agaatatatc tcctacatta gtgctgctat cttcttgctt tatggccttt
    15601 tagctgccaa atggattttt cttgggctag gcttaatttt ggtacttgtc ggccttgctg
    15661 accgcctcaa ttcaaaaaaa taatccttct attcgctcct gtccttttaa cctagcgatg
    15721 tcatcaccag gttaaaagga caggggcgaa cgagggatta tgtttcgttt aaggtaacat
    15781 tacttacgaa aagcgtctaa gatggtttta aattcttcat tagtaagagt aatgcctttc
    15841 cccattttcg tatggtctgg actccacgtg cggatatccc atttcgcttc tgcgccatta
    15901 aagctgacgc ggttcaattc ttttgtccaa cctttatcat tttctgataa ggtgagtaaa
    15961 tgttcctcaa tgttgaatgt aaattctgcc attagttttc ctcctcatct atactattcg
    16021 aaattaaagt ggaaaaaatc atcttttttt cacttttttt tatgactcat tctcctatta
    16081 ttctatcaag ttttgagaaa ataagctatt gtgatatcca actttcaaaa aaaaacaggc
    16141 tctcttttcc caactatggt cgtataccat ttggaaaaag agggcctcta gtatttcttg
    16201 ttattatgtt ctaacagttt tactggtgcc atctttttga tagaggttga caagaccttc
    16261 cttgcaagcc ctaatcatat cccctggctt aacttctctc ggtaaagaga tggttaagag
    16321 ttccattgga tttggggcac ggtcaatctt ttggccatcc gcatcatgta agtccttaac
    16381 aaccgtttca aaatgacgga aacctggtcc ataacattca atccgatcgc cttccatgat
    16441 gacattacgc tgacgaatgg tcgctgtcat gctggctgag tcaaaggcaa ctacttctcc
    16501 gacaaattta tattgtggaa ttttgcggcg agcaccaaat aattgttcat tttcagttgg
    16561 gataccatag taaaaacctg tagccaactc gcgctgggca accttccaca actcgtcaat
    16621 caattcctct ttgatagcat aaaaagcttc tgggctttcc atgtaagcat ctacagccgc
    16681 cttgtaacag ttggttacgg ttgagacgta gtggatagat ttcattcggc cttcaatttt
    16741 taagctatca accccatttt caatcaggtc aggaatatgg tcaatcatac acatgtcaac
    16801 agaggacata gaatagtctt ctggaatttc cccttttaag gagcggcgct ctcctccaaa
    16861 tggcatgtca tacaaatcat acttccagcg gcaagactgt gagcagccgc ccctgttggc
    16921 atcacggtga ctcatgtggt ttgacaaaac acagcggcct gaataagaga tacacatggc
    16981 tccatggaca aaggcttcaa tttccacatc tgtccgctta cggatttctg ctaactcggc
    17041 catattaacc tcacgagcta aaacaactcg ggtcaaaccc atggctttcc aaaattcaaa
    17101 ggtctcgtaa ttggtagatg aagcttgcgt tgacaaatga atttccaaac ctggggcttc
    17161 tgttgaacaa ataacaatca aggctggatc tgaaacaatg accgcatcaa gccccatatc
    17221 acgcagttga cgaaaccact cgcccgcacc aatttcgttc ccttcgtggg taaccatgtt
    17281 ggcagcaaca tagaccttag ctccacgcgc atgtgcataa tcaatgcctt cttgcaattc
    17341 ttccatagag aagttaccag cgcggcttct taggccatag gcctgtcctc caacaaaaac
    17401 agcatctgcg ccatagtcaa tcgcaacttt taatttttca agtgttccag caggtgataa
    17461 gacctcggga cgttttttca tatgtgacat gtgatattct ttctattttc aagataggta
    17521 gattacctgt gtaatggtgt cataaccctg tcttgccctg aaatagtaaa ctcactcaag
    17581 acaaggatta cttatttaac ggtttttgga tcgaactcat aaaagccagt atctaatcca
    17641 cggccagctg gatgatgggc atgcactgct tgatcaagct tttcagcttc ttcttgggta
    17701 aattgcccag cttctaataa ggttttagcc tgaataaaca atttagtaat agcgacaaaa
    17761 tcatcacctg gacagtaaat gccatctagc ttccaatgag ttaagccatg cgcatacagc
    17821 tcacccaatt tagacatcat gtcgatatcg ttattaatga aaatatgagt gccatgattg
    17881 tcttcataaa tggagtaatg gctgctagca tctcctggtt ctgccaagaa aagtcctcgt
    17941 tcacgactaa cctcatcatc aatctttgta aaatggtaat aattttctag caagggacgt
    18001 ttcgagtgat gaatcaccga ggcgccgtaa actagcacct ctgctggaaa ttccaaattt
    18061 tcagctaagg tgaataactc tgctgatggg atttcccttg ctaaaacact ttcaacagca
    18121 ccatgttgcc cccagaaatt tacctgacgg ctagaagtca caaaaacaga ggtatcataa
    18181 atcagtttaa agttatagcc atccctttta ttgacataaa acacacctgc atcaccaacc
    18241 acaagataat caacagcaat ctctatcatt aagtctaaaa agggtttgat ctgatccatc
    18301 atatcttgat gcatcaaagc attgcaagct actgtcaact ctttgcctgc atcatgcact
    18361 agtttggcaa tttgtctcaa ttcatcataa gaaaaattat ggggaagacg taacccgtaa
    18421 ttggcttcac caacataaat acgatcaaca ccagcagcta ataaagcttt gacttgctca
    18481 atgctttcag ctgtggctgt aataattatt ttttccatat aagctttatt ataccaaaaa
    18541 ttttaagacc ttaagcatat ttcttgcaaa agcccaaacg ttaagctagt ccccttactg
    18601 ttatagcatg ataagtcact tgtcattagt aaatcttttc aaatctttta aaaaaagatg
    18661 acaagaagag acttttatga tagaattact tagtatgtta aggaaaggag aggactatgc
    18721 ctgcaccgtt aaagaagcat aattcggttg aacaggatga ttcttaccaa gaatctcaaa
    18781 gtaaatttcg agattttcaa gagttcaatg gccaaagtgc aaagctaaaa gaattagtct
    18841 tctttgctcg tatcgctagt ttcagcctta ttaccgtttt atttgcattt gtcctgctcg
    18901 taatgaattt agcgccaatg tttgctttct tatttgcaag catcatcagc ttaggcatta
    18961 cttccttatt agccaaggtt atcaaatcat tactgatgta acttccagag tccattttgt
    19021 atgactcttt ttcttttgcc aaaaacaaaa aaagattgaa gccacaatcc cccccttctt
    19081 cagttagcct aaaactaacc atcgggacta gtgatcagat ccttcaatct tttcttcttc
    19141 tatgttttag gtcgttttag ttagtgtttg tctaatgtat cactaacagg ctcatcttgt
    19201 tcagtatagt caatgatgat gtcgtcttca tttaaatcaa tgataacatc atcttgcttt
    19261 tctttcgctt caacttcggc aagggtttct tttgtttttt gcacgaaagc tgttgtttta
    19321 tctttgagca agtctaacat gtcctcttta gttaagtcac cagaagccaa cttatctttc
    19381 acatcataaa aagtgtcacg tgctaaatgg ctgtattcac tacctttttc tttagcaaat
    19441 tggtggtaat catctggact ttctttgtag gcttgataag ctttttcagc acgatttttc
    19501 aaagccttgc ctttttctgt tgataagaag taagctgccg ctactcctga aacagctcca
    19561 atcactaagt ttttgaaact tttattcatg cttttctcct ttttttccaa atacttttga
    19621 ggctacttta ccaactaatg ctaattttcc agcttttgaa acattacctg tagcattagt
    19681 tgctttttgc ccaaaatgcc ttgcttgact atttaaatct gaaagacttt ctgacaaatc
    19741 agcaatagca acaaacagtg gatcgatggt tgctacttta ccattaacat cttcaacaag
    19801 gatattagct tttgctaaaa tatcattagt ttgatgaagt gtcacattaa catcacttgt
    19861 caatacagaa atcgtttttt tggcttcatc aattgtttct gagactttct ttaatacaat
    19921 aatcaagaaa atcaccaaag caacaaatgc gagtgcaata atcatcagtg agattccaac
    19981 taagtccata agtttctcct ttttctatcc ttattcttga taataagaaa ttcctttttg
    20041 acggcgtctt tttatgacaa agattaagcc aataataatt aataaggcag acacatattg
    20101 ggagatgcga atacctaaaa acataagact atctgttcgc ataccttcaa tgactagtct
    20161 tccactacca taccaaatta agtaaaatgc aaagatttct ccatctaata gactttttgg
    20221 cttacgacgc cacatcataa taatgacaaa tcccaaaaga ttccaaagtg attcatagag
    20281 aaaggtcgga atgcggtaac ttccctctat aaacatttgc ttttgaataa agcttggtaa
    20341 gtaatttaac tggctcacag ctttaccata ggcttcttgg ttgataaaat ttccccagcg
    20401 accgatagct tgagcgacca tgacacttgg tgcggcaata tctaaaaaat gaatgggatt
    20461 gagcaccttg ttataacagt aagccaaaag tacaagagct cctgtaatga gaccgccata
    20521 aatagcaata ccgccattcc aaatggctat gatttcatct aaatgcttga cataataaga
    20581 ccattcaaaa ataacatagt aaattcttgc accaacaata gcaagtggaa aagcaatcag
    20641 aataaaatcg aaaatagcgt cagatgatat tccttttttg ggtgcttctt ttgatgctaa
    20701 gtaaaccgcc aaaacaagtc ctgacaagat acatagcgca taccagtgga tggctagtgg
    20761 accacatttt aaggcaatgg gattgatcat tgactcacct catttttggt gattagttga
    20821 gttaaacgat cttcaaaagt ttttgtagca tcaaagccca tttctttagc ccgatgattc
    20881 atcgcagcgg cttcaataac cactgaaaca tttcgacctg tttttaccgg aatacgaata
    20941 cgagggattc tcactcctga gaaagttatc tcctcattac cgtttccgag acgatcaaaa
    21001 actttaccag cctcaaagtt ttcaaggtaa atggccaatt gaacttgtga tgagtccttc
    21061 acagcactag ctccataaag cgacatcaca tcaataatac ctaccccacg aatttcaagc
    21121 aagtggcgca agatctctgc tggctctccc caaagggttt cttcatcttt ggcatagaca
    21181 tccacacggt catctgcaac caaacgatgc cctcgcttaa ctaattctaa accagtctca
    21241 cttttaccaa ttccagaatc cccttggata agaaccccca taccataaat atccattaaa
    21301 acgccgtgaa cactagtacg ttcagctaaa gaagcgtcaa gaaaataaga catttctcca
    21361 gctaagcggc tagttgaaac acggctactt aaaagtgaaa taccttcttc ttttgcagct
    21421 tgaaccattt cttttggaat ggctaaattt ctagagacaa ccactgcagg agtgtccttt
    21481 ttgaacatct cttttaaaac agaataacgg ttgtgagaag tcatctgtgt aagataagac
    21541 cactctttca ttccaaacag ctgcaacctt tctggagcat aataatcaaa ataaccagtc
    21601 atttcaagac ctggacgtga aatatctgat gttgttatct cttttgacag caagttatct
    21661 gtagcataaa ccacatcaag tttaactttt tgcactagca tctttacagt aacggtcata
    21721 atttctcctt tttcaatatt ataacacaaa tcgaccgtta gacccttgca aatactcatt
    21781 ttttcactac taaatgagaa caacgacacc aaaaatggtc ttagctctat taaaaactag
    21841 gttttatcat ttagagatat gagtcaaaaa taagacaatg gcaaacaaat actttactag
    21901 tatcattgag caattagtgc ttattatggt atttaaaaag gtatcatctt actaagtcaa
    21961 aaagcaaaaa gcctcatcat agctgataag acccctcgct tacttacata tcaaaacatt
    22021 ttattatcat cagttaatca catggttgct cttacgtgat gaattatcat tttaactgat
    22081 tgtttaccag aaccagccat cttcttcttc atttaagaca tcagcgtctt tacgacgacg
    22141 aggacctcgg ccataacgtt cttcaagcaa gtcttccttg taaggtaaga agatggccaa
    22201 caaaatataa agcaaaactc caaaaccagt tccgtaaata agaagagccg ctaagacacg
    22261 cgctagagca agatcccaac catacttatc tgctaaacca gcaatgacac ctgcaaccaa
    22321 gcgatttttt cgctgtttat agaatttagt ttccacgttt ctttacctcc tcattctatg
    22381 atactagttt acattgtttt tcgcaaaata aaaccagtct tacgactgat ttttttccat
    22441 aagtttcccg tggcaatttc cacaaacata tttagccaga ttaatacgcc tttttcgttg
    22501 ataaacctgc ccacaggttt ggcaactata atgatgatta gtctttgatt tagagctagt
    22561 tgggacataa cgaagcccgt ttacttgagc tagcagatct ttgaaatccc tatctttatg
    22621 atggtaccct cttccttcaa aataaagatg gtaatggcag agctcatggc gaacaatttt
    22681 ccgaaaaata agctcaccgt gctcctcaag catcctaggg ttaaaatcca aatgaccatc
    22741 ttttggaaaa aagcgaccgc cagttgtttt taggcgcttg ttccaataag ccttgtggtg
    22801 aaatggtttg ccaaaatcag ccagggaaac ttcttggaca taattagtga gtgtcacgcg
    22861 gtggcaatag gctgagattg accttgtggc ggtccaagtc aatcttagag acccaaacag
    22921 ttaccaagtc acccactgaa acaacttgac tagggtggtt gacaaaggtt ttgctcattt
    22981 cagaaatgtg aataagccca tcttcatgaa caccaatgtc tacaaaagca ccaaagtcaa
    23041 cgacgtttct cacagttcct tcaagcttct ggccaatttc caaatctttc aaatcaagga
    23101 tatcttgacg taagattggt gcttcaaagt catcacgaag gtcacgaccc ggtttaagga
    23161 gatcagcaat gatatcttta agggtttcct gcccaatagc caatgtttct gccatttggg
    23221 gaacttgaac tgctgctaaa gttgccttgg cagcgtcgtc caagtcctga ataccaagta
    23281 ccttaaagag ttctttgact gctgggtagg actcggggtg aactcctgtg ttgtctaaaa
    23341 tatttttggc tcctggaatt ctgagaaaac ctgctgcttg ctcaaacgcc ttggctccta
    23401 aacgcggcac ttttttgatg tcagcgcgtg atgtcagtgc tccattttct tcgcggtatt
    23461 tgacaatatt ttctgaaatc gttttgttca aacctgacac atgtgctaat aaagatggac
    23521 tagccgtgtt cacattaaca ccgacttggt taaccacagt atccacaaca aaaccaagat
    23581 tctcactcaa ctttttctga ctcacatcgt gctggtactg accgacgcca atagatttag
    23641 gatcaatttt aaccaattct gcgagtgggt cttgcaagcg acgagcaata gaaatagcag
    23701 agcgcttttc cactgtgagg tctggaaact catggcgggc taactctgac gctgaataaa
    23761 ccgaagcacc gctttcgtta acgatgacat aggacgtatt tgggaaatct ttcaaaacgt
    23821 ctgctacaaa ggcttcactt tcccgactgg ctgttccgtt tccaatggca ataatatcaa
    23881 tctggtaagt ctcaatgagc tgagtcagtg tttctttagc tgcttgaatc ttcgtttggc
    23941 tagctggtgc tacgggataa ataacctgag tagtcaacaa ttttccagtc tgatcaacga
    24001 tagccaattt tgcacctgtt cgaaaggcag ggtcaaaccc aagaaccatt ttccctttta
    24061 aaggagacac taacaaaaga tgacggagat tttcagaaaa gaggtggata gctccatctt
    24121 ctgcggcatc actgagttct gaacgaaccc gtctttccat agctggaaca attttctttt
    24181 tgatggtctg attgatgacc tcttcaatat aagggttggt ttctttgaac cgcacactga
    24241 aaaagcgttg cattttctcc aagttatgct caaaagacac ttttaagatg cctaactttt
    24301 cgccacggtt aagggctaaa gtacgatatc cttgcatgtt agacacttgg tcagaaaaat
    24361 cgtaatagat ttggaaaacc tttttctcat ccaactgctc atctttaagc gttgatacta
    24421 agcggctgta ctgccaaatc tcattgtagg tccaagagcg taatttagca tcttctgaca
    24481 tggcctccac aaggatgtcc acagctcctg ctagagcttc ttgtgggctg gcaaatcctt
    24541 ctgtgacaaa gggttctgct gctgtttcaa ggttttgcgc attttgtaaa atcaaacgtg
    24601 ctaatgggaa caagcctgct tcacgcgcaa tcgtcgcttt tgtacggcgt ttttccttat
    24661 aaggcaggta caactcttcc aaatcagcga gtttctcggt tgcttcaata ctcgttcgta
    24721 actgatctgt cagttttccc tgttcctcaa ttttagcgag aatggttgct ttgcgctcat
    24781 tcagggtagt gagagactta tccatatcaa taatggactt aatcaccact tcatctagat
    24841 ttcctgttac ttctttacgg taacgtgcaa taaagggaat ggtattgcct tgtgctgtta
    24901 gggcaagcac ctgttcaatt tgatggagac taactgacaa agcctctgca atgttgtgat
    24961 tattgttatt ttccataata ttgctattat aacacaaacc gtgttaatgc aaaaaatgat
    25021 agcgaaacat tgtcctaata taaagttgcg taaagaatca aaaaaagcta cttttagtaa
    25081 ataactgacg aaaccctaaa taacaatacc taattttaaa aaagaaagaa gtaaacaaca
    25141 cttccgtttt ggcattgttc tcaaatgata gtataataga cttatcttga atgaaagaag
    25201 gtcatagata tggcaattac ccataaaaaa aatgatgaac ttgaaaaaat gctagcaggc
    25261 tttgcatcaa tccctagttt tgataagcct ttggaagtca atactgatgg taaactagcg
    25321 accaaagaaa aaacgacaga acaaacaaaa ccaagcaaac catagctagt ttgtctcatt
    25381 ctcttaacat atcgtccatt cgtttaaact gagttgagag atttttagcg atcaggctaa
    25441 caaaacgtat cagtcgaggc tgaacattcg tgtttcagcc tcctctttta tcatctagaa
    25501 aggatatggt gaatgtcact cttttccaac cttcctccca gtgtcttgca atggtttgcg
    25561 atttttatgt ccattatcat tgaagctctt ccttttgtgc tcctaggaac tattttatct
    25621 ggttgtattg aagtctttgt aacgccagaa ttggttcaaa aatacctacc taagcaaaaa
    25681 tgcttgcgca ttctgtttgg aacctttgtt ggatttgttt ttccttcttg tgaatgcggg
    25741 attattccca tcatcaaccg ctttttggaa aaaaaggtgc ctagttatac ggccgtccct
    25801 tttctggcaa cagcgcctat tattaatcct attgttttgt tcgctactta ttctgctttt
    25861 gggaattctt tgcgctttct gatacttcgc ttggtcggtg ctgcactagt agccattaca
    25921 cttggggtaa tgttagcttt tattgtagat gataacattc tcaaggataa tgctcaaccc
    25981 gtgcattttc atgattattc tcatgaaagc ttgccaaaac gcatttatct agcactggta
    26041 catgctattg atgaattctt cgacaccggt aggtatctcg tatttggaac cttgattgcc
    26101 tctgccatgc aaatatatgt cccaactcgt gtgttgacaa cgattgggca caatcccttg
    26161 acagccattt tgatcatgat gctgatggct ttcattctat cattatgtag tgaagccgat
    26221 gcctttatcg gagcttcctt attatccacc tttggagtgg ctccagttct tgcctttctt
    26281 ttaattggtc ctatggtgga tattaaaaac cttatgatga tggtaaaggc ttttaaagga
    26341 cgcttcattg tccaatttat cggcgtatcc gttcttatga ttgccgttta ttgtttactt
    26401 gtgggggtgc tctaatgatt cgctttttaa ttttagctgg ctattttgaa ctaaccatgt
    26461 atctccagct gtctggcaaa ctggatcagt acatcaatgt tcggtattct taccttgctt
    26521 atatttccat gattttatct ttcattttag ctcttgttca actctatacc tggatgaaaa
    26581 acattaaggt acatagccat ttaaccggaa aaatcgcaag gctgaccagt ccttttatct
    26641 tggtctttcc tgttttgatt ggtctccttg tgccaacagt aaccctagat tccaccacgg
    26701 tttctgctaa aggctatact ttcccattgg ctgcgggagc ctctaaaacg ggagtcagtg
    26761 atgacggtac caccattcaa tacctcaaac ctgacaccag cctttacttt acaaagtctg
    26821 cctaccaaaa ggagatgcgt caagaactcc ataaatataa aggcaagaaa ccggtcacta
    26881 ttaccactga aaattatatg gaagtgatgg aattgatcta tctctaccca gatgagtttg
    26941 ttgatcgtga catccaatac actggctttg tttataacga accgggccat gataactacc
    27001 aattcctttt ccgcttcggc attattcact gtattgccga ctcaggagtg tacggacttt
    27061 tgaccacagg taatcaaacc agctacccta acaacacatg gctaacagtt aaaggtcgcc
    27121 ttcatatgga atatgataaa aacttagaac aacatctacc tgtccttcaa cttgctgagg
    27181 ttcatcaaac caaagagcct aataatcctt atgtttaccg cgttttttaa agactaataa
    27241 aaaagcactt gttttttatc aaaacaaatg ctttttgcgt tttatcagtt agcataggaa
    27301 cagagtcacc atcttaaaca agacctattt aggaaaaatt atcctcaagg taacgtccat
    27361 tggtttgaat gacttgtcgg taccaatcaa acgatttttt cttatagcgt gtgagttgac
    27421 cagtaccatc atcatttcgg tcaacataaa taaaaccgta gcgcttactt aattgggctg
    27481 ttgacataga gacacaatca atacagcccc aagcagtgta acccatgacc tcaacgccat
    27541 cttcaatcgc tttggcaact tgtacgagat gctgactcat gtagtcaatg cgataatcat
    27601 cgtgaaccgt catgctgcca tctgcggttt gcaccagctg atcctttgca cctaagccat
    27661 tttcaacaat aaacaaagga atctggtagc ggtcatagta ctgattcaag accaatcgca
    27721 gaccaattgg gtctatctgc caaccccact ccgaagattc taaatatgga ttggacaaac
    27781 cacctagaat atttccctga ccagatgtgt aagcttcagg attgtgagct tgtgtgacac
    27841 tcatgtaata gctaaaggat agaaaatcaa ccgtatgttc cgctaataat tccttgtctc
    27901 cgtcttcaaa aacaatctca atcccatttt ctttaaaata cgacttaata tatgaaggat
    27961 acttgcctct cacatgaata tctgaaaata agagattaag attttcaaat gcatgcgcag
    28021 caagtatatc acgaggatca gaggtcattg ggtaagcagg catcgccaaa atcatacacc
    28081 caactttaac atcaggattg atttcatgcg ctagctttgt gacactagca gatgctacca
    28141 attcatgatg aatagcttgg tataaatctt gcaggctcag cttttctttt tctgtcatga
    28201 tgccaccact ggtaaaaggc atgtgtaaaa tcgagttgac ttcgttgaag gtcaaccagt
    28261 atttgacttt gcccttatag cgttccatga ctgtctgagc aaagcgctca aaaaagccaa
    28321 tcagacgtct atctgtccaa ccattatagg ctttggccaa atgcagaggt gtctcataat
    28381 gcgacaaggt cactaagggt tcgatgccat agttcaacaa ttcatcgaat aagtcatcat
    28441 aaaattgcaa acctgcttca ttgggctggt catcgtcacc atttggaaaa atacgagacc
    28501 aagcaataga cgttcgaaag actttaaagc ccatctcggc aaaaagagca atgtcttctt
    28561 tgtagcggtg gtaaaaatca atcgcctcta aagttaaatt atcgctggta ggactatctg
    28621 tccattctcc tagtccacca tttggtagaa catcttgtac ggacaaacct tttgcgtctg
    28681 cctcaaatgc tccttccact tggttggcag ctactgcgcc gccccataag aaatcttttg
    28741 gaaaaattcc cattctccct ctccttttag gttataaaat gactaacaaa gcgtcctctt
    28801 tagtcaccat ttttttgtca tcttgaataa taatctccaa ctgctcagcc gaattggtca
    28861 cgaccacagg actgatgagt gaatagcctt ttgaagtaat gaaatcaata tccattttta
    28921 gaagtgcctg gcctcgtttc acgacatcgc caacagacac taactgctcg aaaccttgcc
    28981 cttttaactc aaccgtatca ataccgacat gaatcaagac ttccacacca cgtgtgtttt
    29041 ttaaggcaac agcatgcttg gttggaaaga aagtgatgac ttcaccatca aagggggcat
    29101 agaccacacc ttcgcttggt aatatggcaa atccttctcc taataacttg gaagaaaagg
    29161 tttcatcagg aacttctgat agaaaaagca tctcacctgt cattggagca tacaacgttt
    29221 gtttggttga caagccgctc ttaacttgtg acatatctgc tgcaacactc acttgctttg
    29281 gggactcttc atcaattccc atgaaccaag tcaaactgaa ggctaacaca atcgtcgcag
    29341 ctccggcaat caaagcattt gtaaaattag ctccgcctga tgggttgatg tactgtggta
    29401 aaccaataat agaaggtacg acaaaggtat aagaagcaat attgacaaaa ccaataaagg
    29461 ctccaaccag accacctgaa atcatggctg catagagtgg ctttttaaat ttcagtgtga
    29521 ccccataaag tgccggttcc gtgatacctg ccagcaaagc tgaaatcccc gcggcaaggg
    29581 caacttgacg tgtttgtttc tgctttgttt taaaggcaac agccaaagat gcggctcctt
    29641 gagccaaatt agaagccaac attgctggta aaatcaagac atctggtgtt gcgaccgaag
    29701 cggccaaaaa gattggtgca aaagcccagt gcatccccgt catgacaaca agcggcataa
    29761 tcgctcccaa aatggcaagt gctagccacg gagccacatg ataaatagcg agcatggtat
    29821 tagataaccc ttgaccaata atcacaccaa gaggacctac aaccactaaa gccaaaaagc
    29881 cagaaatcaa aatgattaag gttggctgta agaagctttt catcacgctt ggcaccaagc
    29941 gattgaccca tttttcaatg tattgcatca agtaaaccat catcaaaatg ggaataacag
    30001 atgagccata gctagcagga gtgactggtg ctccaaataa agccaatggt ttaccttctg
    30061 ctaccattgc aacaaaggca ggatgcaata aaatccctgc gatcgtcgct gctaaaacag
    30121 gtgtcacctt aaagcgcgcc gctgctgaat aggcaatcat gacaggcata aaataataag
    30181 ccgcatcgcc aaagaagtta ataaaggcaa cggtttgaga gtccgcactc gctaagccaa
    30241 gcataggaag caagatcccc accactttga gcattccacc accaagaagg gctggaatca
    30301 gcggtgtaat cacaccagca atggtttcaa tcaagcgctc aaggatactt cctttcgctt
    30361 ttgaagaatg atcttcatct tgattgtcaa aatgccctag gcttaaaaag gcttggtaat
    30421 aattattgac atcattgccc aagataattt ggtactggcc gtttttgcgc atcacgccaa
    30481 tcacatttga aatggctttg acttgctgat ctttaacttt ctcatcattt ttcagtacta
    30541 agcgtagacg tgttacacag tgtgtcacac gttgaatatt tgttttgcca ccaaccgccg
    30601 ctaaaatagc cttggcagtt tcttgatacg tcatattaag tctcctttaa atgtttcact
    30661 aaacgtttaa catgaaccgt caaatacaaa agttcgcttg aattaaggtg atagttgtac
    30721 cgctttttta tctcctcact aatatctaag acgcaccgat attctttagg gtatttcgtt
    30781 ttcaccacta taagcagttc tgcatcatca tcatcgtaag acatctctga taacacacgt
    30841 tgagcaaata atttaatatg ggtcataaag cggtaataat cgatggatgt gtcatccaat
    30901 tctgttttga agtctgtttt gattttttgc tcaatataag aaacaatctc cgtcaggcga
    30961 tgtggctcct taaaaggagt gtccaaacta gcattgacaa aatgcatggc aataaaagct
    31021 gcttcatcaa tagctagagt gactcctaga ttacgctcaa ttagctctaa ggcttttacc
    31081 cctagactat attcatcagg ataatagcgt tgaatctccc accgtagtgg gttatgaatc
    31141 aacattcctt gctcatgcct ttcaatagca gagtgaatat gatccgttaa attaatatag
    31201 agaatttcat ctaaggtttt gcctaattta attttaccta gattgataat tctctctgag
    31261 caagcaacaa cttcttgtgg caccgtaata aataactctg tgaagcgatt catattgtca
    31321 ctatttttta aaacaaatga tgtttcgata gcattaagct caatactatc tccaactttt
    31381 tttccaaagg caatcccttt tcccatcaat aaaatatcta atccttgatg gttggtcgaa
    31441 atcgccgcat tatggtttaa aacgcgttta atcaacataa aagtcctagt cctttagtat
    31501 cacaataaaa aatgacctat ccaaaagctc gctaaaaagc acttttcgaa taggtccctg
    31561 ctcacaaaac tgtgataaca tcctttaata ttctgattgt tatgtctcta gcctgcttac
    31621 ttgaagtaat cacatctgag aacgctttaa tgttatcatc tctttaagat tctgtcaagc
    31681 gttttcaaaa aattctctga aagagcaact attgttattc gtatctattg atttagcaaa
    31741 aaagagtttc tgacaatttt aagattatat gatacaataa caatcgatta gataaaggag
    31801 ttatcatgtc acatcatcaa caaaccgttt caaaacaaac aattatggcg attatcgcca
    31861 tagcactcat tggtttttca ggaattttgt ctgaaaccag tatgaatgtc accttcccga
    31921 cactgatgtc agtctatcag ttgcccttaa atagcttgca atggatgacg accatttatt
    31981 tactagcagt ggcgattatg atgaccactt cggctacact gaaaaaaaat gtgcgggaaa
    32041 gacccctctt ttttatggcc acaggtcttt ttacatttgg caccatcttg gccgttctga
    32101 cccagtcctt tgcgatcatg ttgctagccc gcatttttca aggcattggt actggtctgg
    32161 taatgcctca gatgtttaat attattttag agcgtgtccc aatgcataag gtaggtctat
    32221 ttatgggatt tgctggtctt attattagct tagcgcctgc ttttgggcct acttatgggg
    32281 gctttatgat tagccatttt agttggcaat ggatttttat ctgtatactc cctgtaccac
    32341 tgattgcagg tattctagct tattattacc tcgaagattc tccagtaagc gaaaaagtac
    32401 cctttgactg gttggcattt attgcactat cgattagctt aacttctgcc ttattagcta
    32461 ttactagtct agaaaacgga agtgttaatt tgtattactt agggcttttt attctcagct
    32521 ttgtcctctt cctctacaag aatctcacag ctaagcaacc ctttcttgat attcgcattc
    32581 ttaaaattcc ctctctaacc tttggcctga ttcccttttt tgtcttccag ctgattaatt
    32641 taggcataaa ttttctaacg ccaaacttta ttgtcatgga aaagattgcg aatagttctc
    32701 aagctggtat ggtattacta cctggtacct tactcggagc tctactagca cctgcttttg
    32761 gtaaacttta tgatcaaaaa ggagcaagac tttcgcttta tttaggaaat gccttattta
    32821 gtttatcatt gattattatg acacttcaaa caagacattt tatgctttta ccattcactc
    32881 ttttatatat tttattcacg tttgggcgta acatgggctt taataatagc ttagccacgg
    32941 ctattcgaga attgcctgcc gagaaaaatg ccgatgccac ggccattttt cagatgatgc
    33001 agcaatttgc tggcgctcta ggaactgcta tggcatcact gatagcaaat agtcaaccag
    33061 aattcacaag cggtgtccag tctgtctacc ttctctttac tatttttgct ctacttgatt
    33121 ttatcttttt ctttgctatg ttttaccatt tagggaaaaa aggacttgcc taagctgact
    33181 tacttaaaaa ccagcctcgt tctgttgaac aagactggtt tttttcttat aaaatacctt
    33241 ctaaaagacc tttcataaag tcttctgagt ggaattctcc aatatcatcc actttttcac
    33301 caaatccgat gaacttgact ggaatgtcta attcttgacg gatggctagg accaccccac
    33361 cttttgcagt cccatcaatt ttcgttaaaa ttaagcctgt taaaggtgtt atttttgaaa
    33421 attccttggc ttggctaagc gcattttgac ctgttgacgc atctaaggcc aaaagcgttt
    33481 catgaggggc atctggtaga acgcgcttaa tgatacgacc cattttttcc agctctgcca
    33541 tgagattttc tttgttctgc aggcgaccag ccgtatcaat cagaagaata tctacttctt
    33601 ttgccacagc tttttccatc ccgtcaaaaa caacagacgc tggatcagcc ttttcagggc
    33661 cagtaatgac tggcacatct actcggcgtc cccattcgac tagctgagcc acagcccctg
    33721 ctcggaaggt atctgctgcc accaacatga cttttttacc ttcttgtttg taacggtaag
    33781 ccagcttccc gattgaagtc gttttaccaa ccccgttaac acctacaaaa agcataaccg
    33841 tcaagccatc ttgataatta atggcttcat tataacgccc gtctttttca tagatatcta
    33901 ccaatttttc cacaatcaca cgtttcaagg catctggttt tttcgcattt tccagtttgg
    33961 cttcataacg caactcctct gtcaaagttg tcgccacatg tacaccgaca tctgataaga
    34021 tcagcatttc ttccaagtct tcaaagaatt cttcatccac tcgcctgaaa ttggcaaaga
    34081 aactgttcag acgagctgag aagccagtcc gcgttttttt caagctacgc ttgtattttt
    34141 cttgatctgt ctcaggaatg gtatcgtagg ttgccttgtt ttgagaagct tctgctgagg
    34201 tagaggcttg attttcttgt ggaacttctt ctggcatcac tggaaccgtt gccgctgctt
    34261 tttcttgaag gcttttttca agagcagcct tacgacggta gtagtcttcc ataacctgtt
    34321 ggctactgga ttgctctttt gaagtgtcta ctgcttcttc attcgccgaa tcatttgagg
    34381 catcagcttg ggcttgtttt gttggaaact gttccacaac tggctggtca acaaggtctt
    34441 ttggttctga tgccgtagat tgactaagtt cacttacctt aagatctgtt ttgtcggcag
    34501 caaactcatt ggtatcttct gatgaatgat tactattatc acctgtttct tcctcaacct
    34561 gtgcatttac tgaagctgct tcgggaccat tttccaccga acctgcgccc tctgaagaaa
    34621 cgtcctcttg ttgaaccacc tcaatcttgt cttgctcatt ggctttttca gaaagctcct
    34681 ctttctgagt tgtttctgtc aaaagatttt cttctaactt ttcctcagct accttcgggg
    34741 tttctttttt cccaaataaa cgatcaaata atcccattaa ctctcctctt ctaaaataaa
    34801 tgtcttcaca gcttctgcaa ccccagactc atcattgatc aaggtggtca cggcatcggc
    34861 aacggcttta gctcctgaaa caccattagc catagctact ccaagtcctg cccattctaa
    34921 catcgcaaag tcgttagctt cgtctcccat agccatgaca tgtctagcgt caaggccaag
    34981 gtgctgacaa agcaagttta atccaaaagc tttatcaatg ccttttggca tgatttcaaa
    35041 aataatatct cgtgatttaa aggcttcaaa atcctcaaat aagcttgggg atagctttat
    35101 aatctgctga tccaaaaagt ctggatcggt gactgtcaca attttgttat aggtaatgtc
    35161 ttttggtagt tccgctacag atgtcacctc aataaaggtc aacagtgggt tagctagatg
    35221 gtactgcgaa tgcctaccgt ccttacttgg aatgctatac acatccccac cactgataat
    35281 atctgttggt aagccaaccg cctctaaagc ttgctgaatc tggcaaacct ggtcaaaact
    35341 gagagagctc ttatccaaaa cctcccctgt atttcgctga accaaacccc cattaaaggt
    35401 aatactgtag tcatcgtggt caagcaagtc caactcttct agcaagttac cgatggcctt
    35461 tagagggcga cctgtggtaa taaccacctt aacccctttt tctctagccg ctgctaaggc
    35521 ttttttattg gcatctgtga caattttttc agtgttgtag agggtaccgt ctaaatcaag
    35581 cgccaaaatt ctaatattca tgagttacac tagtcctttt aaatacgtta gtactgctcc
    35641 gtcattacag tggccaatga cttggtctga aatcactttg atttcaggtc ttgcattttc
    35701 cgtagcaata gccctaccag caaactccaa catctggtaa tcattaaaat tatccccaaa
    35761 agcaatcgtt tctgctttct taatgcccaa ggcttgacaa agatgctcca taccaaagcc
    35821 cttattcacc tcttttaaga taatatcaat ggaatcaaag ccggtggtca ctgcagtggc
    35881 ataagggagg gcttgattca accaatcgct tccttctaag acggtatggc ctgtgaagtt
    35941 ggtagaaact ttaaaaatgg cgtcattctc catatcttca aaaccattaa taaccttaac
    36001 attagcatag taatgttttg ttttttgaat gtactcttct gatgcgcctt ttaaaatata
    36061 ggctgccttt tgcccagaaa aaaccatacc tgtttctaca tagtgaggat tggctaaaac
    36121 cttttttgca acttcggtat actgctcttt ggtcatcata tctgcaaata gaatctcacc
    36181 acggtattga accacggaac cattttctgc aataacagca atctgatcca aaaagggctc
    36241 aaagagctga tcaatggcta agagcgagcg gccactcgaa accgcaaata aaatgccttt
    36301 ttcggctaac tttggcaaga gcgcagctag ttgctcttga ttataagtac catcttcagc
    36361 taggaaggta ccatccatat ctgttgctat tagtttaatc atacaatcta acctttaccc
    36421 tttttaggga gtgcaaaatt caaatgaatc gtgcatctca actcccaata taacttcttt
    36481 cattatacca cagataaact aaaaaaggac gtgccgaatt gatacgccct tgccttttat
    36541 caatcaatcc gtacgccatt tttgaaggtt aggctgatgt gcttgtctac ttttatatcc
    36601 aacctttcaa cacttgtctg ccagagttct ccctttggac caggagttaa agagatggtt
    36661 gtaaggtcag cttgtgtcgc tagactttcc cctctcacct ttaggagcgg tgtgtcaatg
    36721 gaaagattcc cattcccatc cctatcccaa ctaaagactg gagtattctg tcctggaata
    36781 acgaccttat cagcttcaac ttctaggctc ttcacatact ctgatagaat ctgttgggag
    36841 ataagccttg tcatccatgc tgaactagag acggtcaact catcaatctt agctttttga
    36901 agtgctgcaa ctcgtgcttc taagacatcc gttgccagat aatcaagagc agctttctta
    36961 tcaaaacgct cgccttctgc tttagcagta gcgataccat accactttta tctcgtataa
    37021 tctgtacata ggcatttccc catatcaaaa gatggctcat gattgtctct cgaaagataa
    37081 aggaggacat ctctggattg ggttcatcat gaagaagaaa gtacagtggg tggtccagct
    37141 ttttctcctt cccattttct gtccgttcat agatgtgaat aggaagtgac gccaccgctt
    37201 cagcaagaat acgcacacag gcatagactg ccgtcgtctg catagtctta aactcatcca
    37261 cattctcgcc actaatcgtg cgaccaaaga ggtaggaaaa gtcctgacct tcataactgt
    37321 tttggggctt atctctagcc ctttttcttc caattaaatc aagtagtccc ataagccctc
    37381 cttattttgg ggtacaaaaa aagcacctcc tttgaagtgc tttcggtata tttctttcaa
    37441 agtaatcaac agtctttctt acaaaaccta gtattctcat tctgtcaatt ccttaaatgc
    37501 gtcaagatgt cgtgataata ctgaagtcgc aatctcatct aaccttgctt gttcaacaac
    37561 tgttgtcatc tgctcatcac caaagttcca tcagttgatt tataaaggtt aaactcctga
    37621 ttctcttgaa tgccaaatgg gtcaggtatc ggaagataga tgtcatcacc aatttgtatc
    37681 gtctttacga gttccataca tcactctccc ttactttacc aattattcat atgacgtgat
    37741 ccatagacga ccgcaacaat tataacttca tcttccagaa catgatatac aatgcgatat
    37801 tttttgacaa tcagttgtct gaaggtatag ccttttccaa tcagatcctc aatgatggca
    37861 caacgttcag gaaaaatgga tagtgataac attccttggg ataacctctc aagaagatta
    37921 tccgctgcct gtggtgcaca gagttcatca cgaacatagt gatagatgct cagcaaatct
    37981 gctttagcat catccgacat ggtgacctga tattctttca ttaagcattt tccttcaatt
    38041 gagatagaaa ttctctgaca tcctgacggt tgccagtttt cgcatcttca aagctggtga
    38101 taagcttatc ataaaacttc tcctgactca tgtaatcaac attaactcgt tgaggcgctt
    38161 caggtagaga aacatcaaat gggatacccc cagtcaaaat aatctgattc aaaaacatat
    38221 caatcgcagt tgacatagga atacctaagc gtttcaaaat atcatctgcg gcacttttta
    38281 ctgaatcatc aactcgtaaa ttcaaagttc ccgttttagc catggttatc ctcctttttt
    38341 gtaacgacat tgtatcacgt ttttagagtt gactcaacta aaaactcaat atccctcgct
    38401 catcatagac gctagcttca tcaccttggc ggcgaatgca acggtcaagc cccaggataa
    38461 gacctacaat accgtcaatc ttctcaacag atttttcctt atctggcttg atgttgcctc
    38521 caggggcttg tcgcatcacc acgttttgtc ccatccattt aaggactgga tgaccaccat
    38581 gttagatttt cccttccatc atgagtttat agagttcctt ggacggtgga ctcatatcct
    38641 tataactctg ctcaaaaggc accatggtca agccgacacc gcaattccaa ggtatcttct
    38701 agtaaccatc agagagaaga cagggattgt aaaatcaacc actgtgcaaa atactgcgtt
    38761 cactacccag tcgaatcagc tcaaaaaaca atacgggcag ttcttgttaa cagaatctaa
    38821 taactgttgt gcatgcccag gctgtgatag accactcatt ctaaccagtg gcaacttagc
    38881 agcagaaaat tacgaggttt cagccattga aaaagataag gacgctgaac cactgaatct
    38941 gatagccctc tgcccagatt gttttttaac ctatcaagca gatagtcgca aaaaaattgt
    39001 aacagctcta aaaaatgtta aaaagattct agtctctgca cataacagcc aacaagcgat
    39061 ttctgatatg aagttagaca gtggaattgt ttcagttttg actagtctca ataaactaaa
    39121 atgtgatgaa tatgatatct cctatgactc gaagcaattg actgataaga tatcacctga
    39181 gaacaatcgt acgctttatc aattggttaa aaacccagtt attgataact acttaaccat
    39241 tcaaaaaatc atcgtaaatt tggacaaaca aggaagaatt gattacgagg aaatccaata
    39301 tcaaacgcga tcgatgtata aaaagttaaa ggctgccaag cataataatc tggctatttt
    39361 caatactatt tcggaaaaac tccataaagc tacactacag gacatctact tctgccagat
    39421 gattgtctct tactttatcc aaaaatgcga ggtacttgaa taatgcaatt accaaataaa
    39481 ctttactcct atcaaaaaag cacccttgcc tatttaccta gagtgctaaa tgaaatcaaa
    39541 agtggaaact caaacgtcaa agatatcttt cacgctatct cagaagagtt ggatgatcct
    39601 acagatttcc tatctataat ggattgcttg tatgcattga acgctattga aatgtctaat
    39661 gaaggagaag taaaattatg cttatagaaa tgtggtctcc agtatttaag aatcacggag
    39721 aaattcggga atcaatctgc ttccactatg ggttaaacgt gattatgggg atggatttag
    39781 ctgataactc tatcggcaaa tcctcctcac tattagccat tgatttcatc tttggaggta
    39841 atagctatct aaaatcaatt gcggttaaaa aattaggtga tcatcctatc tacttctgct
    39901 ttcaatttga gaaaaagttt tatttttcaa gagatacggc caacccaaat atcattctag
    39961 tgtgtgataa gagctactct ccaaccggtg aaacaatgga cctaggaatt tttcttaaca
    40021 gacttaagaa acgatatcat ttagattctc cagagctatc attccgatta gctatgagtg
    40081 gcttctttag gattgcagga aaaagtaatc aaaataccga ttttccctta caagtttatt
    40141 cgagccaaaa atcttctgaa tctattacta ctctcatcca actgtttaat ttgtatgata
    40201 acattgcacg atacaaagaa cgtctgaaag ataagagcga ccagttaaca acttttcgta
    40261 atgctcgaaa gtataccttc atctccaacc tggttggcgg taaaaagcaa tttgaagcaa
    40321 atgtctcaga aataaaaaga ttggaatatg atttagcaca tctccaagac actcatcaag
    40381 ataaaattga ctctgatgat attgagaaaa atcaacaaaa actccagctt agaaatacta
    40441 agctggagtt agaaagcagt cttcgggaca agcaaagacg tcttaagcta ttggacatca
    40501 gtatcgaatt tggcttatat cctactgaat ctgacttaac agagcttcaa caatacttcc
    40561 cagatactaa tctcaaaaaa ttatatgaag ttgaagctta ccacaaaaaa ttggaaacta
    40621 ttttagattc cgaattctca actgaacgtg aatccctaat agctgagatc gacgaattag
    40681 agagtcaatt aacaactcta aaccaagaac ttcaagaact aggaaacatc ccaaatcttt
    40741 caagtgagta cttagaaagc tactctaaac taacagctac aatcaatgca ctaaaagaac
    40801 aaaacgaggc ctacttaaag gagtctggtc tatccaaaga aaaatcagaa gcggacgctg
    40861 acttgaaacg cagtacagaa gatatccttc tagatctaga agtaaaaatc aacagtaaga
    40921 tgcgtgaatt caataacatt ctatatccag atattcgcaa agctcctcaa attaacctca
    40981 atgctcacaa tagctactcc ttctatacac cagatgatga tggtacagga actaagttta
    41041 aagggatgat tgtattcgac ttagtaatgc tctaccttac taatctgcca gcactcacac
    41101 acgattcatt actcttgaat aatgttagct accaagcgac tgaagcactt ttgaagctgt
    41161 atgaccagtc aaggtcattg aacaaacaag tctttttagc cttcgataaa gccagctctt
    41221 attctccaga tgctaatcaa ctcttgtcag aaaatacggt attgagactc tctagtaacg
    41281 gaaatgaact ctacggtatt tcatggaaca aaggagaaaa ctcagatgag atttagttac
    41341 aataaattat ggaaacttct tattgataaa ggttggacca aaacaaagct caggcaagaa
    41401 gctgggatta gttcttccac gatcgcaaaa ttgggaaaag gggaaaatat cactactgat
    41461 attttattgg aaatttgtat tgcacttgat tgtaagattg aagatatagt agaaattgta
    41521 gataataata tctagaaata aaaaaagcac taagttgata agatgtgtaa gtcagatcaa
    41581 cttagtgcct ttactttgaa aaaaataatt ttctcaataa tacttagtac taactgttaa
    41641 cgaatattat actttttaaa taatattcta aatactaatg acaatatagt aatgcacgaa
    41701 ccaataataa aaagattaaa gctattgtta ggatttaatg ccaccgagaa aaagccagtc
    41761 ccaataggca taaaaagaat agttattgta aagataatac caaacactct ccctaaaaaa
    41821 tcgttatcaa catctttctg gacaagagaa aaaaattgaa tattaaagat ggaaagaaat
    41881 aaactgaata aagctggtga caaagctaaa ataattgcat tatgaaacat aatataaaat
    41941 gggggagcta acattaacat cagtcctgat aaagatagaa acaatatcaa tctcatactt
    42001 gataattctt tattcacaaa accacttaat attgccccaa taaatccacc aattgcttct
    42061 gctgttaaaa aagtgccata aagtccagtt gaaatttctc caaacatttg attactatag
    42121 ggcaacaata aattataagc ggctagaaaa aaattaacaa gtgcagaaag aacggtaata
    42181 ataaagatgg acttatggct gtaaacatat ttaaatccta tttttaaatc gttaaatatt
    42241 tctctgattg tcaccttctc ctttatcacc acttcgtcat taacaggtaa aataaaggaa
    42301 attagtaaag cagctattag aaatgatagt ccatccaata gtaatacacc gtgtatacca
    42361 agtagcttat ataagaaaat tgctaccata ggaactgtta ctttgattac agtacttgtt
    42421 gtctctagca atgaattaag ttgtgatata ctatcttttt taacaatttc ttttgtaaat
    42481 gctttatagg atggaccaga aaaagcactc ataaatgcca agataacatt agttagtaca
    42541 atggcataaa ccaaccattg ctcttttgtt aggaacgaga gtactaagca agctgtgcca
    42601 cataagatat ttgtagtaat aataattttt ttccgcttga aactgtctgc aatgactcca
    42661 ccaaataaat taaatagaac acctataaca ctttctagag actgatatac tgcaactaat
    42721 gatagagacg ctggattaag cccagcaaga aaagtattgt tagcaaaatc aaacataaca
    42781 tcgccaattc g
//
LOCUS       DAUGFP010000012        40938 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.012, whole genome shotgun sequence.
ACCESSION   DAUGFP010000012 DAUGFP010000000
VERSION     DAUGFP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40938
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.012"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..174)
                     /locus_tag="VH663_000647"
     CDS             complement(<1..174)
                     /locus_tag="VH663_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ8173867.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHKKILVD"
     gene            complement(243..314)
                     /gene="shp3"
                     /locus_tag="VH663_000648"
     CDS             complement(243..314)
                     /gene="shp3"
                     /locus_tag="VH663_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEQ8173868.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            394..1245
                     /gene="rgg3"
                     /locus_tag="VH663_000649"
     CDS             394..1245
                     /gene="rgg3"
                     /locus_tag="VH663_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEQ8173869.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDKDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     SLLDNELNFYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKLHYADHF
                     DKLVNKD"
     gene            complement(1498..5037)
                     /gene="smc"
                     /locus_tag="VH663_000650"
     CDS             complement(1498..5037)
                     /gene="smc"
                     /locus_tag="VH663_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEQ8173870.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFDKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVLSQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHTAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKCVQELERDYQLNQERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGTNRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKALLAALDQSQTTHSDESLLAEQARIEEALTAIAKKKNALTCDIDDIKENKDLIR
                     QKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQ
                     DSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTF
                     IRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQK
                     TIRALGPINSDAINQYEEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKVT
                     FEAIQKSFKETFTQMFGGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGITMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(5038..5730)
                     /locus_tag="VH663_000651"
     CDS             complement(5038..5730)
                     /locus_tag="VH663_000651"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEQ8173871.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
                     YKTCLQEFLQTKGDVVIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            complement(6162..6971)
                     /locus_tag="VH663_000652"
     CDS             complement(6162..6971)
                     /locus_tag="VH663_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ8173872.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            complement(6975..8327)
                     /gene="vicK"
                     /locus_tag="VH663_000653"
     CDS             complement(6975..8327)
                     /gene="vicK"
                     /locus_tag="VH663_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEQ8173873.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWLEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            complement(8320..9030)
                     /locus_tag="VH663_000654"
     CDS             complement(8320..9030)
                     /locus_tag="VH663_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8173874.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            complement(9192..10226)
                     /locus_tag="VH663_000655"
     CDS             complement(9192..10226)
                     /locus_tag="VH663_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ8173875.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRGTLKGFRSPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            complement(10282..11529)
                     /locus_tag="VH663_000656"
     CDS             complement(10282..11529)
                     /locus_tag="VH663_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEQ8173876.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDGELLKKADFAVL
                     SSGTTGKAKLFWRRLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPVGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            complement(11486..12634)
                     /locus_tag="VH663_000657"
     CDS             complement(11486..12634)
                     /locus_tag="VH663_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEQ8173877.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNTVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWTLRSHQKASYCQEQALLADLILDLPGASDQGIRPRLSSKVLSKVPPILGED
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            complement(13029..13178)
                     /locus_tag="VH663_000658"
     CDS             complement(13029..13178)
                     /locus_tag="VH663_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HEQ8173878.1"
                     /translation="MQLLDNVYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            complement(13379..14164)
                     /locus_tag="VH663_000659"
     CDS             complement(13379..14164)
                     /locus_tag="VH663_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173879.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            complement(14166..14984)
                     /locus_tag="VH663_000660"
     CDS             complement(14166..14984)
                     /locus_tag="VH663_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEQ8173880.1"
                     /translation="MWSFWKRYRPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLMSVGFPFLMVLSGIKVMAGLSILQVLASSCLYLVSLLLA
                     FLINFYFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKVVSIVLSFLPF
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            complement(14986..15978)
                     /locus_tag="VH663_000661"
     CDS             complement(14986..15978)
                     /locus_tag="VH663_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ8173881.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNDVLDLNEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQSFGKMKSLSFELKPGQEQVVSQFMGLPDIT
                     VERHELSLDIQYDSSRYQTADIIQKTMADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            complement(16118..18061)
                     /gene="thrS"
                     /locus_tag="VH663_000662"
     CDS             complement(16118..18061)
                     /gene="thrS"
                     /locus_tag="VH663_000662"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEQ8173882.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSRPDAQA"
     gene            complement(18483..19817)
                     /locus_tag="VH663_000663"
     CDS             complement(18483..19817)
                     /locus_tag="VH663_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ8173883.1"
                     /translation="MRVGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGDGPYLQDLKHLAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLS
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            complement(19819..20817)
                     /locus_tag="VH663_000664"
     CDS             complement(19819..20817)
                     /locus_tag="VH663_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ8173884.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADPKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            complement(20948..21949)
                     /gene="ccpA"
                     /locus_tag="VH663_000665"
     CDS             complement(20948..21949)
                     /gene="ccpA"
                     /locus_tag="VH663_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEQ8173885.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            22123..23208
                     /locus_tag="VH663_000666"
     CDS             22123..23208
                     /locus_tag="VH663_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ8173886.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYAEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLIKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKPGVTAAQVDAAARQ
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            23257..23922
                     /locus_tag="VH663_000667"
     CDS             23257..23922
                     /locus_tag="VH663_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ8173887.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEDDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            23933..24310
                     /locus_tag="VH663_000668"
     CDS             23933..24310
                     /locus_tag="VH663_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ8173888.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            24455..25381
                     /locus_tag="VH663_000669"
     CDS             24455..25381
                     /locus_tag="VH663_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ8173889.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMTNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPIMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            25592..26275
                     /locus_tag="VH663_000670"
     CDS             25592..26275
                     /locus_tag="VH663_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEQ8173890.1"
                     /translation="MEWIKLIGIVIIVLGFILKCDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQALTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYGGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            26275..27201
                     /locus_tag="VH663_000671"
     CDS             26275..27201
                     /locus_tag="VH663_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEQ8173891.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            27251..27898
                     /gene="pcp"
                     /locus_tag="VH663_000672"
     CDS             27251..27898
                     /gene="pcp"
                     /locus_tag="VH663_000672"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEQ8173892.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERIAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(28016..28735)
                     /locus_tag="VH663_000673"
     CDS             complement(28016..28735)
                     /locus_tag="VH663_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEQ8173893.1"
                     /translation="MSIMITKGQVRLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(28741..29208)
                     /gene="smpB"
                     /locus_tag="VH663_000674"
     CDS             complement(28741..29208)
                     /gene="smpB"
                     /locus_tag="VH663_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEQ8173894.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQESDIKKQMKHYNAR"
     gene            complement(29211..31544)
                     /gene="rnr"
                     /locus_tag="VH663_000675"
     CDS             complement(29211..31544)
                     /gene="rnr"
                     /locus_tag="VH663_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEQ8173895.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNGVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSALDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINPQGHVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILEAMRSKRGALNFDTQEAKVIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKVEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYNQPSQEKRDHFAQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGIK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            complement(31638..31874)
                     /gene="secG"
                     /locus_tag="VH663_000676"
     CDS             complement(31638..31874)
                     /gene="secG"
                     /locus_tag="VH663_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEQ8173896.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            complement(31920..32066)
                     /gene="rpmG"
                     /locus_tag="VH663_000677"
     CDS             complement(31920..32066)
                     /gene="rpmG"
                     /locus_tag="VH663_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ8173897.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            complement(32063..33256)
                     /locus_tag="VH663_000678"
     CDS             complement(32063..33256)
                     /locus_tag="VH663_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEQ8173898.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVAPILSLYIRHLGQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            complement(33378..34880)
                     /locus_tag="VH663_000679"
     CDS             complement(33378..34880)
                     /locus_tag="VH663_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEQ8173899.1"
                     /translation="MTTMTIAKGNQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISEMGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNIAKQSIGAIQRSLLTLEQEGAHQFFGEPALDVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVTQSPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSEI
                     ESQQLVQSSPFASKYSQSIDRESAYEKLAAKVLEDNRLAQEAIATAQREKEAKEAIKA
                     QAATKKANRRSVGRPRKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            complement(35070..35663)
                     /locus_tag="VH663_000680"
     CDS             complement(35070..35663)
                     /locus_tag="VH663_000680"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEQ8173900.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPLLMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNNGDMETLIKQVQSALLSLANPR"
     gene            complement(35660..36487)
                     /gene="mutM"
                     /locus_tag="VH663_000681"
     CDS             complement(35660..36487)
                     /gene="mutM"
                     /locus_tag="VH663_000681"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEQ8173901.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLITKSQLSAFFAKRKLGPEPKKETFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPETASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRP"
     gene            complement(36659..37525)
                     /gene="rgg2"
                     /locus_tag="VH663_000682"
     CDS             complement(36659..37525)
                     /gene="rgg2"
                     /locus_tag="VH663_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEQ8173902.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            37614..37682
                     /gene="shp2"
                     /locus_tag="VH663_000683"
     CDS             37614..37682
                     /gene="shp2"
                     /locus_tag="VH663_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEQ8173903.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            38596..38865
                     /gene="stcA"
                     /locus_tag="VH663_000684"
     CDS             38596..38865
                     /gene="stcA"
                     /locus_tag="VH663_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEQ8173904.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHS
                     KQNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(39213..39611)
                     /locus_tag="VH663_000685"
     CDS             complement(39213..39611)
                     /locus_tag="VH663_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEQ8173905.1"
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLE
                     DDTAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLTHHLLHSDGVLPSLNDKSA"
     gene            complement(39704..40777)
                     /locus_tag="VH663_000686"
     CDS             complement(39704..40777)
                     /locus_tag="VH663_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173906.1"
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGV
                     GYKEETTSQFFSDQPSQVVLYRGEANTVRFTYTDQMYLMKDIRIALDGSGKSLTAQTV
                     PGMGHVYEGFQTSAREIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGALPDNIK
                     VSINPKGGGLATITYGAGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRD
                     QSLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEV
                     GSWLEPATALPSVEMSAEDRLKS"
BASE COUNT        12317 a         8702 c         7190 g        12729 t
ORIGIN      
        1 gtctaccaaa atttttttat gtttatattc atccagaact cctatgttgt gatttagata
       61 ttcaaataat tcatctgata tagaatgttc aactggatta ccaagcaatg cagaatacat
      121 aaatttcatc ctcctacgtt taattagttt actactaaca taaagtacaa gcattacaca
      181 gagctaacca ttacctataa gtctttagag tatctaaaga cttaggtgta cacctaagta
      241 acttacccac caacaataat gataatatcc attgctaaaa ttaaaatcgg caaaaatttt
      301 gaaattttct tcataagcgg acttcctttc agtttttatt tattgtacaa taaataaaaa
      361 ctgaattgat ttttgttggg aaagtgggaa aatatgaaat caaaacttgg tgccacactt
      421 agaaaagttc gaaaaggaaa gcaaattagt ctgtgcttcg tggcagatga acacctttca
      481 aaatcacaaa tctctcgttt tgagcgcggg gagtcagaga tatcgtgtat ccgactgatc
      541 aatattctag acaaattaca cattactttg gatgagtttc ttattttaca tgataaagac
      601 tataccaatt ccgaaacatt tgctaacctg gtacagtata ttcgtaagca atattcatca
      661 caaaatatca ataatatagc atgtttactt tccgacactt ctgattacac tctgaattct
      721 tttgagaaaa caatggtaaa gtccatttta cacacaatgg attcaagcat cattccatca
      781 gatgatgaat tacttcaact aacagattat ctctttaaag ttgaaaaatg gggctactat
      841 gaaatccttt tgttgggtaa ctgtgtacga acaattaact acaactccta cttcctattg
      901 acaaaggaga tgttaaataa ttacatttac tcttcacgga acaaaactaa taaacgaatt
      961 gtcactcagc ttgctataaa ttgtttcatt cttagtatca ataaggagga attttctaat
     1021 tgctcctatt taattgatga gataaaatca ttattagata atgaattaaa cttctatgaa
     1081 cagacagttt ttctctatgc aacaggttac tttgaattta agcgccaatt agaaactgga
     1141 gttgaaaaga tgaaacaagc catacaagtt cttgatattt taaatgaaga caagttaaaa
     1201 ttacattacg ctgaccattt tgataaacta gtcaacaaag attaagtaaa atcattcttt
     1261 accttttaac gaaacgccaa tcaagggtgt ctcaataaaa aatcatctac tctgacaact
     1321 tttctctttt acctatcgac agctaaaaag ccttggaaac tgctatttcc aaggctttct
     1381 ttgtactcta ttgtatcaat tagcttattc cgatttctgt tgctattagt ttaatcatga
     1441 aatctcctaa cttatggccg aacatccaaa aaaacagaag gaactccctt ctgtttttta
     1501 attggtcatc tcttgagctt cttttaattt tacagataca attttagaga cccccgattc
     1561 ctgcatggta attccataaa tactatctgc tgcagccatt gttcctttac gatgggtcac
     1621 gacaataaat tgactatctt tgtcaaagcg attgaggaaa tccccaaagc gcttaacatt
     1681 tgcttcatca agagccgctt ctacctcatc taaaatcaca aacggaatcg ttttaacacg
     1741 tataatagca aacaataaag ccagtgctga caaagctttt tctccgcctg acatcaagtt
     1801 aagagattga attttcttac caggaggctg cacagaaatc tctataccag cagacaagag
     1861 gtctgtttcc gttaaaacta aatcagcaga acccccacca aacatctgag taaaggtttc
     1921 tttaaaactt ttttgaattg cctcaaaagt cactttgaaa cgagccttca cttcgctatc
     1981 catactatta atagtctcta gcaaaagatt tttagctttt gtcagatctg tcttttgact
     2041 tgttaaaaaa gtcaaacgct catgcacttc ctcatactga ttaatagcat cactattaat
     2101 tggtcctagt gcacgaatag tcttttgtaa atggtgtaat ttttctttgg cactttctaa
     2161 gtgatcaata ctatttgtta cctcctttgc gtctgctagt gtcatttgaa aatcttctga
     2221 taaactttta gcataggctc gtaagcgatt agccacttgc tctaaatgtg tctctaactt
     2281 ggtttgacga cgaataaatg tgtcattttt ttcgctttct ttggttattt tttctgctgt
     2341 ttcttctaag cgggcctcat agtcctctat ttcaaatctt aattggacta aacgtttttg
     2401 agcaccagac ttatgctctg tggcatcttg taactgtttt tgccactggg gtagtctttg
     2461 aatgctgtct tgacttacgt tattgtttag tatgctttca agtttgagaa tattttgctg
     2521 gcattgcttc agctgagttc ttagacgaga ttgattagct tgctcaaatt tcttttcatt
     2581 aagtaaatct ctttcttgca aacgagcttg agataaagct tggtgaatgt tttgtgtttt
     2641 ttgcctaatc aaatctttgt tttctttgat atcatcaata tcacaagtta aggcattttt
     2701 tttcttagca atagctgtta atgcttcttc aatacgagct tgttctgcca ataaagattc
     2761 atcagaatga gtcgtctgac tttgatcaag ggcagctaac aaagcctttg aatcttcttg
     2821 tttttcttgc aattgctgat aagccatttg agctctttgt tcagctaagc gtgcctggtc
     2881 accggctaat ttcaattgtg tcagttcttc tttttttgct atcaaatcgc tttgcaaggc
     2941 agctacttct ttttcagtta tttttaattg ctcaactaat cgcgttaatt cctcactgat
     3001 ttgttctaac tctggtttga taaaagttgt attgctttgt ctattagttc ctcctgaaaa
     3061 agaaccacct ggtcgtaatt ctgttccatc aagagtaaca attcttacct tatatcctaa
     3121 taagcgcgct gcaatattag cttgatcaat agtttcaaag atagcagtac tagataaaag
     3181 gttttggata atagctgaaa gactatcatc atagcgaacc aatgattctg cagttcccag
     3241 ataaccttca caagtcgcta attgacgatg atagtgttct gataatgatc tagcctttat
     3301 agtagttaaa ggtaaaaaag ttgctcgtcc ttggcgattt tttttcaaat aagcaattgc
     3361 tctttttgct gcagcttcgt ctgttacaat aatatgctga ctattagcac ctaatgcaat
     3421 ttcaagagct gtttggtaat cagaatcaaa agaaaggtgt tcactgactg cacctatgat
     3481 ccctccaagc tttttttgtg attgaaggac agctcgtact cccgcataga attgactatg
     3541 acttttttga atagattcta agctagcctt acgagcttct tttccttttt tttgatccaa
     3601 caagtcaaat agtctttctt gattcagttg gtaatctctt tctaattcct gaacgcattt
     3661 atcaccttct tgataatttt ggagcaacat ttcgacttgc tctttttgag ctttataatg
     3721 agcttgagct ttttgagact cgtcattaag ttgatcaagc ttagtaacaa gtagctgata
     3781 ttcttgagcc gtatgttgac gagcctgttt ttctttgtct aaatgggctt ttaaagctgt
     3841 cagctgattt gataaggccg cctctttttg catcaataat acaaactctt ctcttagagt
     3901 ttccattaat tggtcgggat cacttgaaaa acgctctaat gcattgctca attcgtttaa
     3961 ctgttgtttg acatcacaaa gttgttgatc aatatgcaat aattgttctg tacgctgctt
     4021 ttcttctgct tgaaaaccat ccaattgttc ttgtaactgt tcgagatgtt ttttggcttc
     4081 tgcctttttc tctgcttctt ggccactttc tagcttaacc aattcaattt gcttttccaa
     4141 gtcagctatt agcttggtta attctaataa tgttgtttgg gtttgatctg actcttgact
     4201 aagaacctgc tttttttgct tgaatttttg gtaatcttct tccatagatt gacgcttagc
     4261 gtaataagat gctaagtctt gttgtaaagc agctaaagct tcagtatctt tagtctgtct
     4321 ttcttgagct atatcaatat ctttgactag aatatctagt tgtagttgct tacgattggc
     4381 atcaagttct aaaaattgtt tagctacttt agcttgcttt tcaagtggtg ccaactgcgt
     4441 gtctagctca taaataatat cctctagacg gtctaaatta tcttgagttt gatttaattt
     4501 aatctgagtt tcttttttac gtgttttata ttttaaaaca ccagctgctt cttcaaaaat
     4561 ggcgcgcctt tcttcaggtt tactattgaa aatttcctct acacgacctt gagaaataat
     4621 ggagaaagaa tcacgaccaa gccctgtatc cataaacaaa tcatgaatat cacggagcct
     4681 taccttgcga ccatcaatca gatagtcact atctccattt cggtaaatat gcctttcgac
     4741 acggatttct ttcttggcag tttttataaa atgatcggaa ttatcaagaa caaccgctac
     4801 cttagcatag tttagaggat tacggttttg tgtcccagca aaaatgacat ctggcatctt
     4861 gcccccacgc agatttttag cacttgattc tcctaaagcc caacgtaaac tttctgtaat
     4921 attactttta cctgaaccat ttggtcctac gactgcagtt actcccttat caaattcaat
     4981 tttagtcttg tccgcaaagg acttgaaccc ttccaactca atttctttta aaaacatcta
     5041 aacctcacta agttgggcaa gggcattttt agcagcatct tgctccgcta attttttaga
     5101 cttacctaaa cctttactaa ggacagcacc attaacaact atcgatacct caaattgttt
     5161 agcgtgggca ggtcctttct cactaatgac ttgataatca ataaccacat caccttttgt
     5221 ttgcaaaaac tcttgaagac aagttttata gtctttaacc ctttcaaaat tccctttttc
     5281 cacctgagga atcatgactt gtttcaagaa gcgacgtact gcatcaatcc ccttatctaa
     5341 taaaagagca cctaaaaagg cttcaaataa gtctcctaaa atagtatcac gacggcgacc
     5401 acctgacttt tcctcacctt ttcctagttt gatataagcg tcaaaagaac aaaatcgtga
     5461 aaaacctgcc aagctttcct cacgtacaat catggaacgt aacttagaca tatctccttc
     5521 tgtcttttta ggatactttg caaataaata ctctgaaata atcaattgta gaacagcgtc
     5581 tcctaaaaat tccaagcgtt cattatgtga aacgtttagg agacgatgct cgttagcgta
     5641 agaggtgtgg gtaaaagctg tttctagtaa agtcaaatca ttaaattgaa tatcaaaaga
     5701 tgttgagagt aactcttcaa gctgtttcat aaaaaatccc tttcacattt gtcagtagac
     5761 cttatttagt ataccaaaaa tccacctaga aaaccgactc taagtggatt tttcgtgatt
     5821 tattaaacat cttaaatacc cgtcaaagga aactttctca tacaatcaaa agactctaca
     5881 ctatctgagg ggaatttccc actacagata gtatagagct attttgtttt tagtctgaac
     5941 aactaaaaaa ctaccttatc tttataatcc tcaacacctt tgataaatag atcagacaag
     6001 ccaaaactac aggctaattt aagtctattg atgctctaaa ggatgaccaa tgagagttca
     6061 ttgaactcag aagacaatac gctttaagac atcaaaaaat agccctgact ggtatatcac
     6121 caatcagcgc tactttgtat attctctttt agtttcttat ctcaaatatc agttaatgga
     6181 caagcagtat ctggagaggt atcatggacc gtaaagtctg tacctactgc taaatctgcc
     6241 atagccagtt gattgaccat cgtcatatgc gctaactctt tgatgttatt ttctttactc
     6301 agatgaccta ggtatatttt tttggtattg taccccaggc ttctaatcat ggctcccgct
     6361 ccatcttcat ttgataaatg tcctaaatca gataaaatac gctgcttcaa gctccaagga
     6421 taagagcctg atctcaaaat ttcaatatca tgattggatt caatcaagta gccatctgca
     6481 ttttcaataa tacctgtcat cctatcactg acatatcctg tatctgtcaa catcacaaaa
     6541 gacttattat ctttcataat ccgataaaat tgaggatcaa tagcgtcatg gctgactcca
     6601 aaactttcga tatcaacatc tccaaaggtc aaaactttat ccctttggaa aatatgtttt
     6661 tgtgaggcat caagcttacc tagcatattg cactcatcca tcagctgcca tgttttttca
     6721 ttagcataga tatccaaatg gtatttgcgg gccataacgc ctacaccctt aatatggtca
     6781 gagtgctcat gggtaatcaa aatagcatcc aaatcctcag gtttgcggtc aatttcagca
     6841 agaagactgg taattttctt accagtaagc cctgcatcaa tcaataagcg ttttttaggt
     6901 gtttctaaat aaaaacaatt tcctgtggac ccagatgcta aaatactata tttaaaacca
     6961 ctctcattca ttgattagtc tacatcttcc tcccattctt catagatggc tgcatctttt
     7021 tcataaggca agacaatagt aaaggtcgat cctttaccat agtcactctt agcccagata
     7081 aagccatggt gctgcttgat gatttcttta gcaatggcaa ggcctaggcc ggtccctcct
     7141 tgggcacgac ttcttgcctt gtctacacga tagaaccgat caaaaataag aggcaaatct
     7201 gttttaggga tacctagtcc ttggtctgaa atggaaataa ttaattgggt atctgttgtt
     7261 ttcatacgga ctgtaatttt tccaccatct ggagaatact taatggcatt gttcaaaata
     7321 ttctcgataa cctgtgtcat tttatcatta tcaatttcaa gccagacaga ggtaataggg
     7381 taatctctta caatttcata gacttttcct gtacctgtat gctgattttt aaccaaatca
     7441 aatctgttta aaatagaagt tataaaagca gtaaaattag tcatctctac tgctaattgg
     7501 gttacttgat tatcaatccg agacaggttt aaaagatctg aaatcatacg catcatccga
     7561 ttagtttcat caagagaaac ttttatgaaa cttggagcaa tatcttcttt aagtgcacct
     7621 tcatcaagag cctccaagta ggatttaacc gaagttaaag gggtccttaa ttcatgactt
     7681 acattagaga caaaaagacg gcgttcacgt tcttcttttt cttgttctgt ggtgtcatgg
     7741 agcaccacaa ccaagcccga aataaaacca ctctctctcc tattcaacgc aaagcgcaaa
     7801 cgtaatgaga caaactcacc catatcattt cggctattaa cagttaccac tggtgttttg
     7861 gataccaaat cacggtaggt atatgaagta tcaccttcta acaaatctgt aatgtttttc
     7921 tttagtgcct cttctttact taaatttaat tgcttgcgag ctgtctcgtt aatcatgatg
     7981 attttaccag accggtctgt agcaagtaca ccatctgaca tataagccaa aatacttgcc
     8041 aagcgatttt tttcttgggc aagattttca tgcgtcaagc gaaaaacatc tgacaggtca
     8101 tttaactggt ctgaaagctc cattaactca atgtctgctt tttcgtcgat tatatcagta
     8161 tagcgaccat taatcaagtc tcggatttta tggctcatca tccgaataat acgtgcattt
     8221 cggtagtcac gcaccgcaag atggataaag taaaaagcaa caaaaacaag taagataagg
     8281 atcgctaatt caaatgtgga taagtttcca atgatatctc tagtcataag atttcatgta
     8341 gtacccaaca ccacgtcttg ttaaaatata ctcaggacga cttggtgtgt cttcaatttt
     8401 ttcacggaga cgacgaacag tcacatcaac cgtgcgcaca tcgccaaaat aatcatatcc
     8461 ccaaacaatt tccaataagt gttctcgtgt catcacctga cccatatggt tagctagatg
     8521 atgcaatagt tcaaattcac gatgggtcaa ctctacctct tgaccatgtt ttttagcaac
     8581 aaacgcatct ggtaaaatct gtaaattacc aatggttagt tcctgtgtac ctgaagaagc
     8641 attttcttct gcaacagcag tttcaatagt ttcggtacga cgcagatgag ccttgacacg
     8701 tgccagcaat tcccgattag aaaagggctt ggtcacgtaa tcatcagccc caatttcaag
     8761 tccaataacc ttgtcaaact cactatcttt agccgacaac ataataatcg ggacatgact
     8821 ggttttacga atttccttgg ctacttcaag accgtccaac tcagggagca tcaaatcaag
     8881 aataattaaa tctggctttt cttcttcaaa aattgttacc gcttcgcgtc catcaaaagc
     8941 tgtaacaatg tcataacctt cttttgtcaa attaaactta ataatgtcag aaatcggttt
     9001 ttcatcatcc acaataagta tttttttcat gtctatacct cagaaagttc ttgtaattga
     9061 tattacaatt tgtgattttt gtggttaata aaaaatcaca aaactatatt tttctaaatt
     9121 atttctacct gattatacca aaaattcagc ctaattgaca atgacgacta ccgttacaaa
     9181 tagagtactt tctaaaacca accctttata ggatgaattt gacgtttgaa ttgcttagca
     9241 gattttttga ctctggcaaa actactcttg ttgtggtaag caaaaagtcc tttggcatta
     9301 aaataagaga acataaagtc atctttttga gctctaaacc actgacgaaa tactcttttt
     9361 ggcgatcgaa atcctttaag ggtgcccctt ctaaactgag gatcgtgacg gctaatcgga
     9421 tcaacatcta aatcccaatg tctaggacca tctgtgccat agttaaaaga tgcaccattg
     9481 acaattcctt tttcggtcac tacttctgca tcaacttcat taagaaaaaa gcctgtgcta
     9541 tctagaataa attcggtatg aaaccaaaga agtactttta tattatagga aacttcttga
     9601 cttggaaaaa tgactttatt atcttcaaat cttagtttat tatgtaacct ggcagaatcc
     9661 atcaaggtat aatctggtgc ttgccgccac aaacaaggcc cttttttaga cctcaaaaaa
     9721 agggccagag cttgggcgtc tgtcatctca atcattttat aatggtttct gatagattga
     9781 gcttgttgac tagagatgac ataacgaaat tgatgcaaac gcttttcaag ctcacctgct
     9841 tggcttagtg ttttgccagg aaaagcttga tcaactaagc tagccaactc tttccaaaac
     9901 atatgatctg gcaatagaga tggatggaat cgggtgagta gcgtttgagt ttcatgcaac
     9961 tgaccactta gctcaaatgg catctctacc aaatcaagaa gaaacgttag taactgctta
    10021 gctgattggt tttctttttc aataggccat attacccaga aagttggaga accaagtcga
    10081 ttatcaatga taggctcttt agctatagca cgaaatgctt ggtttgataa acctgcgttt
    10141 tgatagcatt tcaaggcctg atagcgatct aagatagctt gtttcatggc cttctcctca
    10201 tcttttttga ccatattata acacatctaa agctgaatcg tcacttgtat ctcactataa
    10261 gcaaaacgaa acctgaagtg gttaactaag ccactgtttg ggtattttat ttaaaagaac
    10321 ttctcgatta attttaccat tatcttttaa aggcaaacaa tcaatcacaa gatagtactt
    10381 gggaagttgt ccagagttta aataaagagc taaaaagtct ttaataggtg ctaggcaatc
    10441 cttcttagtc aaaactaata ctaatattag ggtttcgttt tctccttttc ctatctttaa
    10501 agcgtgcgct tctttgacgt ttggcgcttg gtgagcgagt tcaactaggc taggcaggtg
    10561 gcatttcact cctcgttgat taacccagtc atcttgtcgc ccttctaaaa ttaaacccac
    10621 tgggctcatt tttcctaagt ctgagacact gtatggttgt gacattcctt ctacactata
    10681 aggagtctct acaaaaattt ccttatcttt gaaactgata gacacatcag gaaagggctg
    10741 tcctaccaaa ccattaattt tgacagcagc tctgccatta caccacgtta taaaagataa
    10801 ttcactagcc ccgtagaaaa tgacaatttc aagctgagga aattttttgt aataatgtct
    10861 tagtaattct tgtgatatca tttgcgaaga ggtcaataag tgagtcgcag tcatgttgtt
    10921 cttagtcaaa taaggtagca agcgatttag gtaggtcggt aaaaggtaaa ggtgactcac
    10981 cttttttgct tgccataagc ttaaccacgt tttcaaagac agcttttggc ttaaaaccag
    11041 acatcccccg gcccatagtt gagctaatgc taagttaaga ttgccagtaa aactaaaact
    11101 accatgtaag aatagacaac tattaccagt cataccaaac accttatttt gataatcaaa
    11161 aagtctcgtc caagttgata gacgacgcca aaataatttt gctttgccag ttgttcccga
    11221 actcaaaaca gcaaaatcag cttttttaag aagctcacca tcgacgtgtt ttatttgctg
    11281 ctgaaatgta cctgagatat tagggtgtaa aatgatagga caactgcctt cttttaatcc
    11341 tcgtaaaaaa gagagtaact gattaagata gcgactatga ctgatgatat atggtacact
    11401 gtcttttatt gtctgatcct ttattaaaac cgcttgccat agctcttgat aagtcaagct
    11461 tatctgatcg gctacgatag cttttttatt tgggcattgc tttgcccaat attctagctt
    11521 tgtcagcatg actaaactcc ttatgatatt ttagtaaaac agcaaaccct tggccaccag
    11581 ctgctgctat agcagctatt ccatagcgtc catttttaat ctctaaagcc ctcattaagt
    11641 gcaatattat aatggctgct gaggcaccat agggatgacc ataagctaat gcaccaccaa
    11701 atatattata gcggtccaat aaatctggat aatgggtttc aaatagacca tcaataacgg
    11761 caaaggcttc gttccattca atcgctgtca tatctgccat ccctagacca tgtttttcca
    11821 aaaggacttg actagcctta atcaccatta aagggctacg ctgtgggtct cctgctactt
    11881 ctaccacatc aatcagttta aaagctgaag gctgactact tagctgtaaa aaggcagcag
    11941 catcatgggt taggcaggca ttggctgcac taatcacgtg atcttctcct aagatagggg
    12001 gtacctttga caacacctta cttgacaagc gcggtcgaat accttgatca ctggctcctg
    12061 gcaggtctag aataagatca gctaacaggg cttgctcctg gcaataacta gccttttgat
    12121 gacttctcaa tgtccagtga ttcaaatact cctttgtaaa gccatgctct cttgccactc
    12181 gttctgcccc ttcaatcatc gcaaaagggc tgatactatc tggagaaaac tgagctacct
    12241 tgtaggctcc ttggcgccaa tcagctgaag catataccga ttcaggttgt aaggagctgc
    12301 tttcaatccc tcccactaaa aggttgcttg ccatacctgc cttgatttta gcataaccca
    12361 cggaaagagc tgcaccagct gatgcacact gcatatcaac cgtcaaagca gagacagatt
    12421 ctcctaaatg agaatacagg gtcatcaagc gcccaatatt accacccgtt ccgacggtat
    12481 tgccacaaat cacttggtca atgggaactg gatacttatt ttgtaaagcg ttgatgagct
    12541 ttgctccaag gatttctggt tgttctttag caaattgttt ccctactaaa ccgatagggg
    12601 ttctaaggcc tgcagcaata taaacatccg tcattatatt gacctccttt attttcgatg
    12661 tccttaaatg gttattatat aattatttct gaaggttttc ttttttgaac taataactgc
    12721 taaaaataca cctaaattaa tttagagaat ctctttagtc tcgctagtgt gccatcaata
    12781 aaatctataa aattctctaa cgtcattgaa atgtcaagtc ccacaactat tgtcgtagaa
    12841 gtcacttact ttgttgcttt aacattcaaa ctgtaacctt tgtttttgat acagggtgac
    12901 tttattatac tggaaaacca ttcctaagaa aagaaaatct tatatagaat aaagaattag
    12961 acaccataaa aaagctaagc tgtgacactt aactttttta tggtattgac aaaccttgtc
    13021 tttactattt attgatagtt aatgcctcta actaaaataa aagccatgtc tgtatcagcc
    13081 tgcaaggcat ctgctaagct ggtatcgtta ctagatttga caggtctcat aaaggcaaaa
    13141 gatctcaaag ttccataaac gttatctagc aattgcatgc aaccacttga aatatatctg
    13201 cttatgttat tttaccgccc ttaatttaga ccaatctgct gatgatggtt ccaaccaatg
    13261 cctacacttg gtaaaccatc acaaaaaaaa gcagccgttt ttacaacagc tgccttcctt
    13321 cattatctag attttcatat tctgacctaa acgattttca atttgtttaa gagtctctct
    13381 aggaacctgc actttcataa gcatccaggc cccatttcca aagcttaagc gacaaggcca
    13441 aaaccagtaa cgacaccact accaagcctc caatattaaa gagcaggtgt tgacctgtta
    13501 aaaaataaga agcagggtaa taagctgtaa aagcaaaggg aatgataaaa ctgattaacc
    13561 accgcaaaaa ggaatggtaa atggacatag ggtacttaga aaagtcattg aacatgtaaa
    13621 aaatatagat aactgcccca gactgcttgg tccaaaaaga aatgctggcg gtcgcaattt
    13681 ttaaagaggt gtaaatcaag gtcgcaaaag gaataaccaa aataaacaaa agcaccttag
    13741 gaagagtcca tacgatacta ccagccgtgg tcactaataa gataacccca accaataatt
    13801 cacccaaggc atccacttga aatgtctcaa ccaaaacatg aaataaagga ctaattggtc
    13861 tagtgaggta cttgtcaaat tctcctttgc ggacaagccg ttgccctaaa gcccaaagat
    13921 tgtcaaaaaa gaggtgatca attccctttg gaatcaaaga aaagccataa ataaaggcaa
    13981 tttgttcaaa cgtccagcct tctaaagaag ggatatgttg gaaaagtacc gataaaaaga
    14041 gcagattaag tccctgagtg agaaaaacac ctagcactcc caccacaaaa tcaaccttgt
    14101 attccataat ttgtttgagg tattgtttga taaaaatagc gtgcatacgt ctcaattttg
    14161 ccatctcacc ctccttgaat cgttaaatgg tactgaacct ttttccagat gacttgcgat
    14221 aagactacca tcaccagtaa ccaaaagatt tgcaaactta gggccaccat tatctgacta
    14281 aggctgtatt tcccaatgac aatcataact ggcgtgtaaa ccaaagagga aaacggtaga
    14341 aaactaagca ctatagaaac cactttagga aaaaaagcca atggaatcaa actgcctgac
    14401 ataaaggcaa ccaaggcatt tttcaataaa ttagaccccc aaaggttttt aaagacaaat
    14461 gctgaaaagc caaaacaaat gttgaaataa aaattgatta aaaaagctaa taaaaggcta
    14521 actaaataca gacaactgct ggctaggact tgcaagatag acagccccgc catgacttta
    14581 ataccagata agaccattaa aaaaggaaag ccgacactca ttagcacaat ccagcgaaaa
    14641 ccaatttcca taaagaggta agaggctgca aaatgaactg gtcgaagcag gcgcatgatg
    14701 atagaaccat ctttcacttc ttcgccaatc ataaaggagc tgtctgattt ggtcagtaaa
    14761 gtggtcacaa aactcatgat aatgtaaaag gtcatgtcag acagagtaaa accattgatt
    14821 aaggattgct tagacgaatc aaaaacagcc ttccagaggt aataagccac aaaagccccc
    14881 atgacatctc caatacgata caaaaagaaa ttaacgcgat aggttattaa ttcctgaatt
    14941 cctgcactga gaaagggtct ataacgtttc caaaatgacc acatcttaaa gctcctttcg
    15001 gtagaaacga cgaacaatat cctcaatatc aacatcagtc atcttcaaat ccctgaccgc
    15061 aaaatcagcc atggtttttt gaataatgtc cgctgtttga tagcgcgaac tgtcatattg
    15121 aatgtctaaa ctaagctcat gccgttccac tgtgatgtca ggtaagccca taaactgtga
    15181 gaccacctgc tcctgccctg gcttcaactc aaaagacaga ctcttcattt ttccaaagct
    15241 ttgctttaat tgggtcaccg ttccatcaaa gatttcttgc cctttatcaa tcatgataat
    15301 ccgatcacag agctgttcaa tatcactcaa atcatgggtc gtcagtaaaa tagttgtctc
    15361 ttcttcttga ttaatttgag taatggcacg ccgaatgttg tccttcacag aaacatcaag
    15421 tccaatcgtt ggttcatcta aaaacaatac tttaggatta tgcaacaagg aagcagcaat
    15481 atcagctcgc atccgctgac cgagtgataa ggtccggaca ggatccttaa taaactcatt
    15541 caggtccaaa acatcattta aaaaatccat gcgcttgcga aaggcttttt caggcacatc
    15601 gtaaatttct tttaacacga cataggtctc ttgcaaggcc aaatcccacc atagttgtgt
    15661 tcgttgacca aaaacagctc caatgtctct cacataatct tgacgattat cttgtggaat
    15721 ctttccgtta atgcgacagt aacctgatgt aggctttaaa atacctgtta acatcttaat
    15781 cgttgttgat tttcctgcac cattggctcc aataaaacct aaaatttgcc ctttgggtac
    15841 ctcaaaagat aagtccttaa ccgcctcaaa gatttctctt tggggatgaa caaaggactt
    15901 caaggctccc tttaatcctg gctctttaat ggttttggaa aaattttttt gaaggtggga
    15961 cacttctatc atgaccattt accattcctc gtttctgtta tcgtcttatc accgttacta
    16021 aacaatttat tataacacca tgaaaaagac tgtgcaatcg tttgcttaat ttttaagaaa
    16081 gaaaaagaag cccataagga cctctttttg ccattgatta agcttgtgca tctggacgtg
    16141 acttgcgagc aatgtctgcc aagatgtttt ccacaaattc ttccacagat tcagtgtgag
    16201 ttgccttgct accatagcgg cgaacattga ctgatttgtc ttccatttct ttgtccccaa
    16261 caattaattg gtaaggaatt ttgctggttt gagacgcacg aatcttgtat tgcatttttt
    16321 cattgcggtc atcgacatct gcacgaacac cacggtcacg aagggttttt gctacttccc
    16381 aagcatagtc aatgtgggct tcatttgaaa ttggaatcac ggttacttgg tgaggtgcca
    16441 accaagttgg gaaagcaccc ttgtaagttt caataaggat agcagtgaaa cgttccatag
    16501 ttgagataac accacggtga atcatgactg ggcggtgttc ttcgccatct gcaccaatgt
    16561 atttaaggtc aaagcgttct ggcaacaaga agtccaactg aatggttgat agggtttctt
    16621 cgttaccaag agctgttttc acctgaatat caagcttagg cccataaaag gctgcttcac
    16681 cttctgcttc aaagtaatct acacccatct catcaagtgc agctttaagc atactttgag
    16741 cattttccca catctcatca ttgtcataat atttatgagt atcgttaggg tctcgatatg
    16801 acaaacgaaa acgataatca gtcaagttga agtcagcata aacatcaatg attaattgaa
    16861 gggctcgttg aaattcttcc tggatttgct ctggtgttac gaaaatgtgg ccatcattca
    16921 aggtcatttc acgtacccgt tgaagtcctg atagggcacc tgatttttcg taacggtgca
    16981 tcatgccaag ctcggcaata cgaataggca attcacgata agaacgcaca tgatttttat
    17041 acacttgaat gtggtgtggg cagttcattg gacgaagaac aaactcctca ccatctccca
    17101 tatccattac agggaacata tcttcttgat aatgatccca atgacctgat gtcttgtaca
    17161 attctacaga agcaagtggt ggtgtgtaaa cgtgttggta gcctgatgct aattctttat
    17221 ctgtgatgta gcgctcaagt gtacgacgaa tagtcgctcc atctggtaac caaaatggca
    17281 agccttgacc aacttcttga ctaatcatga acaagtcaag ctctttacca agcttacggt
    17341 gatcacgttc tttggcttct tctagacgag tcaagtaagc tttgaggtct tttttatcaa
    17401 accaagctgt tccatagata cgttgcatca tgttattgtc gctgttacca cgccagtaag
    17461 cacccgccac atttagcaag tggaagactt ggatacggcc tgttgatggc acatgagggc
    17521 cacggcaaag atccacaaat tcaccttgac ggtaaaccgt caagccagca ccagcatgct
    17581 cattaatcaa ttctaccttg taaggatcat ctttgaacaa ttccaaagct tcttctttgg
    17641 tcacttcttc acggatacat gggtaatttt ctgtcacgat tttttgcatt tcagcttcga
    17701 tacgtggcaa atcttcgttt gaaatttggc cttctgcatt atctgtatca tagtaaaagc
    17761 cttctgcaat ggcagggccc actcccaaat gaagatttgg gaacaagcgt tttgcagctt
    17821 gggcaaataa atgggctgca gagtgacgaa gcacctcata agcatctttg tgatcaggtg
    17881 tcacgatttc aatactgcca tcttcttcaa tggcacgagt ggtgtcaatg agttgatcat
    17941 tgaacttacc agctaaagct tttttagcca atgatttgct gatggactca gcaatgtcaa
    18001 acgttgttac gccagattca aattcacgca cggcgccatc tggaaaagta atcttaatca
    18061 tttgctttct cctttaagtc agttaagatt tccactaaaa catggatagt aagacaaatt
    18121 agggacaaga ggttagtgaa tagctcaaca ccaccacgta gaagaacaat accttattca
    18181 cgaatctacc ctccctagtc cttaaaaaca aggctaatcc cctccaaaac aaaaaaactg
    18241 accgcatccc aaaggacgca atcagcgtgg ttccacctta atttatgcag gacaaaaaac
    18301 atgccttgca cctctagtgg cgataagggg gccacccttt tatgtttgca taaaaaccag
    18361 gaaggagtat cagattagct atccttgtgc tctttccacc aaccagcact cgcttaaaag
    18421 attctgtcca atctaacttg tcttcaagat aattatagca agttttcaaa aatttacaag
    18481 ccttaatcca gaaaatcttt gatagcatgg accagcttga taggagcctt gactactttt
    18541 actgaggtga tagctgtgac cttagcagca cgtttaggca agtgaatagc accttgcact
    18601 aattttagag aactactttt accagaatga ttaagataaa gactcaactt ttgagcttct
    18661 ttgctatttc tagcaattaa cgtatctaaa tagaacgtgt aaatagattt tccaaagtgc
    18721 tgtgctgaga tttcataacg tttttttgct aataaccgtt tatccataac tggtgttttt
    18781 agtatggcat caataatagc atcagttaaa tctgtttcag cgtaataaag agtgccaaac
    18841 attttatcag tcaccacatc atctaaataa ggattgccat gagcaataat aggagtgcca
    18901 ctagccaaac tttcaatata ggtcaagccc tgagtctcac tggtcgatgc tgagataaag
    18961 aaatcacaag ccttatagta cagagcaacc ttatcatgag gcaccatgcc tgtaaaggtc
    19021 acgtgtttgt caacctctaa ctgcatcgcc aagtgtttca aatcttgcaa ataggggcca
    19081 tctcctacaa taataagctt tatcttggca ttttcagcca aaatagctgg catctgattg
    19141 atgatagctt gaatattttt ttcataagaa atccgtgaca aactcaataa catggtttca
    19201 tcaccagcaa tgcccaattc tgcttttaag ttggttactt cttctgctgt gatgtcatca
    19261 cgaatatatt tttccaaagg aatgcctgtt gggataaccc gcttagggat agtcacttcg
    19321 taaccttcta gaagattgag gacaatccta cttgggcaga taaccccatc caaatccttc
    19381 aaatagcccc taagaagagg tttgaccata cttggtcgaa tgatttttcc gttggcaata
    19441 taactcacgt agtcctcata ttgggtatgg taagtatgga caacaggaat tcgcaaagct
    19501 ttgcctatca ttttccctaa taaacctaag ctaaactcag tttgcgtatg aataatatca
    19561 agattatagt gttttgcaat tttgtatgac gaaatgaggc cacgataaac cacacgtcta
    19621 tccgtaaatg acacaaaagg aacacttggc agtcgaataa tggtcgggtc ttcaaagcgt
    19681 ttgacatctc tatcagtagt ggtgaaaata taaacttcgt gaccttcttt ttctagctct
    19741 tcttttaacg tacgaatact agtagcgact cctgaaactt gtggaaaata ggtatctgtg
    19801 aatagaccta cacgcatctt ataactccat tactttttta tagacatcta ctaaggcatg
    19861 gccaaccgtt tctaggcgac gactctgggc aaccttgtaa ccagcttcaa ctttgttgct
    19921 ggcaccagaa aagacttttt tcagagcttc tataaaacct ggtatatcgg ttgctaattc
    19981 cgcactactt tgatcaaccc atccgtagta aactggtata tcacgtaaaa caaggtgctg
    20041 gcgactggcc aaggcttcta agacaacaat gccttctgtt tcttcacgac ttggaaagaa
    20101 aaaggcatct gcaccagtca tggcaccttc ataaacatcc cctttaatgt atcctgggaa
    20161 aataagattt ttcgggtggt taccattgac catttggcga acttgagcag gaatgaccca
    20221 tttgttggtc tcgccaaacc agataaaacg aacatctggc atagcttggg caactttgac
    20281 aaagtcatca attcctttcc tcagaaaaaa taatcctgct cccataacca ctttttcacc
    20341 ctctttaatg tcaaagtagc gacgaaaagc tgcttccttt ttaggatctg ctccgtactg
    20401 ctccaaatca atcccatttg acactgcaaa aataggtgac ttcactccat aactctcaat
    20461 caaagactta gaatataggg taggggtaat gatagcatct gccttattgt aaaagtgaca
    20521 aaggtatttt ttaaaccaag gagataatag atttgaaaaa ataaaagaat ttctaaaatc
    20581 ttcttctgta gaatgcccat gcatgataac cttcttacca gctttttgtg cttttatcat
    20641 cagcagccaa ctttttaaac cataggtatt gagatgaacc aaatcataag tttctcttgg
    20701 attagtcgta aaatgttgac caacaagtga caaggcttta gcctgatgct taatcgctcg
    20761 accaattcct gattttctta gataattttc tgcttctaaa tacaataaga ctttcataag
    20821 ttctattata gctcatttta tagtattttt cactaagaca tgcaaaaatc agccttaggg
    20881 ctgattttag tattgaagaa acagtctatt gtgatacttt ttgttagatg gtgctcataa
    20941 gtcacagtta cttagttgtc ccacgttttt taataccatg attcaaaaga atttcttttt
    21001 cttccaactc ttctttgttc atgattttag tcaacatccg catgctaaca gcacctaagt
    21061 cataaacagg ttgactgata gaactcaaat tgggacgagt gtattgaacc actggtgaat
    21121 cgttgctagt gatgatttca aagtcctctg gtacgcgttt acctgcttca aacaagccgt
    21181 ttaaaaggcc tgctgccaat tcatcttcag ccacataagc cgctgttgct cctgagttaa
    21241 tgacgcgctg cgccaattca aatccttctt tataagaata gttcgcttca aaaacaagac
    21301 cttctttgaa gtcaagctta ttgtgcttca acccttcttt gtaccctgct aagcgcactt
    21361 taccattgat atcatcaatg agtggtcctg acacaaaagc gatacactta tgattttcag
    21421 ctaaaatatc aacaacgttt gacaccgcag ctctatagtc aatgttgacg cttggtaatt
    21481 gatgatcaag atctacagtt cctgccaaaa caactggagt acgagagcgt gaaaattcgg
    21541 cccggatttt ttctgtcaag tgatgcccca taaaaataat accatccact tgcttggcaa
    21601 aaagggtatt gacaacatta acctccttgt catcatcttc atcactggag gcaagcacaa
    21661 tattatattt gtacatagcg gcgatatcgt caataccctt agctaagata gaaaaataac
    21721 tatttgcaat atttggaatc acaacgccaa cggttgttgt ctttttgctg gcgagaccac
    21781 gcgcgacagc attgggacga tagtcaaggc gatcaataac ttctaaaact ttcttacgtg
    21841 tattttcctt aacattttta ttgccattaa caacacgact aacggttgcc attgaaacac
    21901 cagcttcacg ggcaacatca taaattgtaa tggtatcatc tgtattcatt ttttatcttc
    21961 ctttctattt gaaaatgacg ctttcaaaaa gtattttagc atgatttgta accactttca
    22021 agtgttatag gttaattttt gaaaaagttt tcaaaaatta actcgaagct tgatttttta
    22081 agcttttttt gaaaaaatag taagtagaaa gaaggttagc ctatgacaaa attagaccaa
    22141 atacgtttgt atcttgacca aaagggagct gaattggcga ttttctcaga tcctgttacg
    22201 attaactatt taactggatt cttctgtgac ccacatgaaa gacaactgtt cttgtttgtc
    22261 taccatgatt tagctcctgt gctttttgta cctgcccttg aggtggccag agctagccag
    22321 gccatatcat ttcctgtgtt tggttatgtt gattctgaaa atccttggga aaagatcaag
    22381 gccgttttac caaatacagc agccaaaacc atttatgctg aatttgacca ccttaacgtc
    22441 aataagttcc atggcttgca gactatcttt tcagggcaat ttaacaatct aaccccttat
    22501 gtccaaggca tgcgcctcat caaatcagct gatgaaatca ataaaatgat gattgccggg
    22561 cagttcgctg ataaggctgt tcaagttggt tttgacaata tttccttaga tgctaccgaa
    22621 acagacgtca tcgctcaaat tgagtttgaa atgaaaaaac aaggcattca taaaatgagc
    22681 tttgacacta tggtcttaac tggaaataat gcagccaatc cccatggcat tcctggaaca
    22741 aataatattg aaaacaatgc cctacttctt tttgacttag gtgttgagac cttaggctac
    22801 accagtgata tgaccagaac tgtggctgtt ggtcagccag accaatttaa aatcgatatc
    22861 tacaatctct gcctagaggc acaactagcg gctattgact ttatcaaacc aggtgtaaca
    22921 gctgctcaag tagatgctgc tgctcgtcaa gtgattgaaa aggctggata tggtgagtac
    22981 ttcaaccacc gcttgggaca tggtatcggc atggatgtcc acgaatttcc ctccattatg
    23041 gctggcaatg acttggtcct tgaagaaggt atgtgcttct ctgttgaacc tggtatttac
    23101 attccaggaa aggtcggtgt gcgcatcgaa gactgtggcc atgtcactaa aaatggcttt
    23161 gaagtcttta cccatactcc aaaagagttg ctttattttg aaggctaagc ccctaccatc
    23221 tggttaaaca aaccctcgcg aaaggaaggt attcgtatgg atcaaaccat tcatcatcaa
    23281 atacagcaag cactacactt tagaacagcc gttcgtgttt ataaggaaga aaagatttct
    23341 gatgaggatt tagcccttat ccttgatgct gcttggttaa gcccttcttc tgttggctta
    23401 gaaggctggc gctttgtcgt tttagacaac aagcctatta aagaagaaat caagcccttt
    23461 gcctgggggg cccagtatca actagagaca gctagtcact ttattctttt aatagctgaa
    23521 aaacatgcaa gatacgatag ccctgctatc aaaaatagcc ttttacgacg tggtatcaaa
    23581 gaagatgatg gtctcaacag ccgcctaaaa ctctatgaat ctttccaaaa agaggacatg
    23641 gatatggcag ataatcctcg ggcactcttt gattggacag ctaaacaaac ctatatcgct
    23701 cttggtaata tgatgatgac agctgctctt ttgggcattg atacttgccc tattgaaggc
    23761 tttcattatg ataaggtcaa tcatatccta gctaagcata atgtgattga tttagaaaaa
    23821 gaaggcattg ctagcatgtt atccctaggc tatcgcctgc gagatcccaa acatgctcaa
    23881 gttcgtaagc ctaaagaaga agtgatttca gtcgttaaat aaggagatta aaatgaaagc
    23941 attacatacc tgtatccgcg tcaaagacct tgaccagtcg gtagcttttt acacaagcgc
    24001 cttccctttt aaggaaaact atcgtaaaga ttttccagat agtcaattta ctcttgttta
    24061 tctggcttta gaaggagaaa gttatgaatt agaattaacc tacaattatg gtcatggcga
    24121 ctacgactta ggaaatggct atggtcatat tgcccttggc tctgaacatt ttgaagcaga
    24181 ccataaaaaa catcgacaag caggttttcc agttaccgat atcaaagaac tggctgacaa
    24241 atctgcccgc tattatttca ttcaggatcc agatggctac aaaattgaag tcattgattt
    24301 aaacaactaa tactattaac aacagaagct ttaaagcttc tgttgtttta ttttttaacc
    24361 tgacgattgg gacaatctca ttattaacag agttttgaaa tatctcagct tttgtgttac
    24421 aattgcccta aaattccatt tgaaagaaaa gtctatgaca ctactatcta tcattgtccc
    24481 ctgctttaat gaagaagcaa acattctccc ttattttgaa gaaatgcatc aactagaaac
    24541 gtccatgacc aaccaacttg cttttgaata catttttatt gatgatggtt ctaaggataa
    24601 caccttgggt attttgcgtg agctagctgc tcgctttcca aatgtgcatt atctgtcttt
    24661 ttcacgccat tttggcaagg aagctgggct cttagctgga ctaaaagaag ccaaaggcaa
    24721 ttacattact gtgatggatg tggatctgca agatccaccc gaacttttgc caattatgta
    24781 cgctaagcta aaagaaggtt atgacatcgt tggcaccagg cgccaaaata gacaaggaga
    24841 accactaatt cgttcaatgt gttcgaatct attttatgga ctgatcaaac acttatcaga
    24901 tactgaaatg gtcaatggag ttcgggatta tcggttaatg accagacaag tcgttgatag
    24961 cattcttgag ttgggagagg ttaatcgttt ttcaaaaggg atcttttcgt gggttggtta
    25021 tcgtatcacc tatcttagtt ttgaaaacca aaaacgcaaa tatggaaaaa gtcgttggca
    25081 tttttgggag ttgctccgat attctttaga tggctttatc aacttttcag aaatgccgtt
    25141 aaccattgct acctggacag ggactttcag ctttttgatt tctattttcg ccatcttatt
    25201 tatcattatc agaaagatac tctttggtga tcctgtctct ggttgggcta gcactgtctc
    25261 tattatttta ttcatgggag gcattcagct cttttgcatg ggtattattg gaaaatatat
    25321 ttcaaaaata ttccttgaaa ctaaaaaaag gcctctctat attatcaaag aaaaacatta
    25381 aattaaaaca tttttctcat caatcgtttc ctactccttc tgtgcgcgaa gctttctttt
    25441 taagcccgtt ataccaaatg ataatgacct ttccaaaata gtatcacctt ccttaacact
    25501 ttaagtagaa aaggaactat taggttgagc ctttttgcaa atcctagcga ctaatgttat
    25561 aatcgtcata ctattgaaaa aggagttctt tatggagtgg attaaattaa tcggtattgt
    25621 gattatcgta ttagggttta ttttaaaatg tgatgctatc gctaccgtag ttgttgcagg
    25681 acttgttacc gcgctggttt caggaatttc gtttattgat tttctagata ttttaggaaa
    25741 agaatttacc aatcaacgtt tgctaaccat tttcttcatc actttaccgc ttatcggctt
    25801 gtcagaaact tatggactga aacaccgagc tactcagttg attcaacgtg ttcaagccct
    25861 taccgtcggc cgtttattga ctctctacct tattattcga gaattagcag gtctcttttc
    25921 gattcgacta gggggacatc cacagttcgt tcgccccttg attcaaccta tgggagaagc
    25981 tgctgctaaa gccaacattg gagaagagtt gaccgatgca gaaaaagatg acatcaaagc
    26041 tatggcagct gccaatgaaa actttggcaa tttctttgcc caaaatacct ttgttggcgc
    26101 aggaggtgtc ctacttatcg ctggaacttt agaacagctt ggctatggtg gtaaccaagc
    26161 taaaattgca ttttcttcta ttttaattgc tattatctct attattatag ttgctattta
    26221 caattacctc tttgagaaaa aaatggaacg tcaacatcag aaaggagata actaatggct
    26281 aacattgcaa accttgtttt agaaacgata tatatcctta tcggcttgca actgtttcac
    26341 acagcctact gcacctttaa agataaaacc aaccctgtct attttgggac agctctgttc
    26401 tggggattgc tgggtgttac cttcgttggc ggtgcctttt tgcctaacaa agttatcggt
    26461 tttatcgtga ttgtcctagc tcttttaacc ttgtttaagc aagttcgtat tggaacatta
    26521 ccagccttta atgaacaaaa agctgaagaa agtgctcacc gcattggtaa ctggattttc
    26581 ttaccagtta tgttaatggc tatgatttct ctacttcttg ccttgatcct tcctgatttc
    26641 agtaaaagtg ccattgggat tgctggtatc ttagccacta ttgctatttt aataatcact
    26701 aagcaaaaac catctgctct tttagctgaa aataatcgga tgaaccaaca agtgtccaca
    26761 agcggtattt tgccacagct cttaggagct ttaggagcta tttttgcagc tgctggtgtt
    26821 ggtgatgtga ttgcttctct cattcgggag attgtacctg ctgatagccg cttctttggt
    26881 gttctcgctt atgtgcttgg catggttatt tttactatga ttatgggaaa cgcttttgct
    26941 gcctttaccg ttattacaac cggtatcggt gttccttttg tttttgcttt aggagctgac
    27001 cctattattg ccggtgcttt agctatgact gctggttttt gtggtacctt attaacacca
    27061 atggcagcaa actttaatgc cttgcctgtt gctttgatgg agatcaaaga tcgaaatgca
    27121 gttatcaaaa agcaagctcc tatcgccctt gttttaatta ttagtcacat tgccttaatg
    27181 tatttacttg cttattatta acttttttgc taagttagta gcgtcagtta ttcattgaaa
    27241 ggacattatt atgaaaattc ttgtaacagg ctttgatccc tttggcggcg aagctattaa
    27301 tcctgccctt gaagctatca agaaattgcc agcaaccatt catggagcag aaatcaaatg
    27361 tattgaagtt ccaacagttt ttcaaaaatc tgccgatgtg ctccagcagc atatcgaaag
    27421 ctttcaacct gatgcagtcc tttgtattgg gcaagctggt ggccggactg gactaacgcc
    27481 agaacgcatt gccattaatc aagacgatgc tcgcattcct gataacgaag ggaatcagcc
    27541 tattgataca cctattcgtg cagatggtaa agcagcttat ttttcaacct tgccaatcaa
    27601 agcgatggtt gctgccattc atcaggctgg gcttcctgct tctgtttcta atacagctgg
    27661 tacctttgtt tgcaatcatt tgatgtatca agccctttac ttagtggata aatattgtcc
    27721 aaatgccaaa gctgggttta tgcatattcc ctttatgatg gaacaggttg ttgataaacc
    27781 taatacagct gccatgaacc tcgatgatat tacaagagga attgaggctg ctatttttgc
    27841 cattgtcgat ttcaaagatc gttccgattt aaaacgtgta gggggcgcta ctcactgact
    27901 gtgacgctaa taaacctatt ttaaaaaaaa cagagatatg aactaactct gttttttttg
    27961 tgctaaaaat gaaagaccta gggaaacttt tcataggtct ttctcaattg tcatcttaat
    28021 ctaatactac ttctaacatc agcgggtata gtttgccagt aattaagaaa cgttgttgat
    28081 ctaaatgagc aatcccattc aaaacattaa ggtcagggta atgggactta tcaagattta
    28141 aggcttttaa caaaggacta atatcatagg tggctaccac ctttccagaa tcaggttgga
    28201 gtttgacaat agtattggtt tgccaaatat tggcatagag ataaccatct acatactcta
    28261 attcgttaag cattgagatg gggacacttt ctatagcaac tagtacggta tccaataaag
    28321 caaaatcttt ggggtctcgc ttttgcaaaa aagcatttcc actagtcatc caaagacact
    28381 ggttttcctt atcataggct aatccccagc catctccttc aaatggatag gcccctaaac
    28441 aattgcaggt agccttatca aacttgtagg ctacaccctc tttataggtt aataaccaaa
    28501 aataatcctc aacaactgtt agcccttctg caaaaactgt atctggaaaa gctatttttt
    28561 ctgaaaagat ctcttgagta aggtcataca cccctacttt tgagaaacca taacgaccgg
    28621 cactaagtaa tatatgattg ttgttgagtt gttctaatcc ctgcgtgtat aagttgctat
    28681 cataagaata agttcgtaac aaacgaactt gtcctttagt tatcataata ctcattcacc
    28741 ttaccttgca ttgtagtgtt tcatttgttt tttaatatca ctttcctgat cacggcgttt
    28801 aatggtttcg cgtttgtcat actcgtgttt ccctttagct agaccaatca gaactttagc
    28861 aaagccatcc ttgaggtaaa ctttgagagg aaccaaagtc atgccagatc ctttaagttc
    28921 atttgctaga tgcgtaatct cacgtttttt gagcagcagt ttgcgggttc gttcaggatc
    28981 agcgttccaa atattacctt gttcaaaagg tgcaatatgc acgttgacca accacgcttc
    29041 cccattctta atttgggcaa agccatcttt cagttgaata cgggctgcac gaacactttt
    29101 aatctcagtt cccgttaaga caatccctgc ttcaacggtt tcaacaatgt gataatcatg
    29161 tctagccttt ttattttgcg ccagtatatg accttcccct tttgccatag ttaactcctt
    29221 ttacgactct tcttttttgc ttgttctttg taaaatggtt ttttagaccc ttttttagtt
    29281 ttacccttgg tttttgcctt ggctacttct ttaggttctt ttttctttat ccctttagat
    29341 gattttgagg atttacggcg atctcgtcga ctagctttat ccgacatctt gattttttca
    29401 accacatcaa aatcgctagg taaatactca aagtcaatgt caccagtttc cttgtcagcc
    29461 ttaaccaatt tcacacgaat aggttgacca actttaaaga ctttacctga tttttcacct
    29521 tgaaggctta gcgttctctc gttaaagtga tagtattccg gtaagctagt gatatgaacc
    29581 aaaccttcaa tcgtatttgg caattctaca aaaaaaccaa atttaacgac actggccacg
    29641 ataccgtcaa attcttcgcc gacatactct gccatatact cagctttttt catagcttca
    29701 accacgcgtt cagcatcaat agcacgacgc tctaactgtg aagaagatgt tgccagctct
    29761 ggaatgatct gagcaaaatg gtcgcgcttt tcttgactag gttgattgta ctcacgaacc
    29821 atgcggtgaa ccaacaagtc agggtaacga cgaatcggac tggtaaagtg agtgtaatat
    29881 tctgcggcca aaccgtaatg cccatgatta tgttcagaat atcttgcttg ttgcatggaa
    29941 cgtagaagca tcatgttgag cacttctgct cctggttgac cttcgacttt tgccataaag
    30001 gcttgcaagg cttcctgaga aatcttgttg gctgttcctt gaatgtgaat accaaaggtg
    30061 ctagcgtagt caataaattg ttgaagtttt tcaacctttg gttcttcgtg aatacggtag
    30121 ataaacggta acttagcttt tgcaaaatgt tctgccacac actcattggc tgctagcata
    30181 aaggattcaa tcatccgctc tgcaattccc ctttgacgaa gcaccacatc aaccggcatt
    30241 cctttgtcat taacaataac tttagcttct tgcgtatcaa aattcaaggc gcctcgtttt
    30301 gaccgcatag cttccaaaat cctatgcaaa gccaccatga gggtcacatc atcagcaatg
    30361 ctagcgaatt cttggagcgc ttcttcatct cctgcaatca tgtcattgac cgtactatac
    30421 gtcattctgt aagttgtctt aatcactgat tgacagattt gataattgac cacatgcccc
    30481 tgcgggttaa tttccattaa ggctgactgg gtcaaacgat caacgttagg attcaaagag
    30541 cagatgccat ttgacaaacg ctctggtaac atcggaacca cacgatcggt gacatagaca
    30601 gaagtgccac gggcgatagc ttccttatca agggcagatc cttctgtcac ataatatgag
    30661 acatccgcaa tatgtacccc aagttcatag ttaccgttat ccaatagctt aatatggata
    30721 gcatcatcta agtctttggc gtccgcacca tcaatagtaa tggtcgtttc ttgacgcaaa
    30781 tccacacggc ctattaaatc tttagcagtt ggtgcttctg aaatagcatt ggcctcggct
    30841 aaaacttctg ctgggaactc tgacacgata tccatcgatt caaggacttc taggacatca
    30901 attccaacgt ctccttgatg gcctacgata tcacgcacac tggctacaaa ataatcgtgc
    30961 ccgcgtgtcg ggtatttatc aatatcaacc ttgatgattt cagtgccttt aagcacaaca
    31021 ggttcttttt tgatatagat tttttgttga atcttttggt ttttggacct gatataacca
    31081 gcatattttg gtttgtcatc atctaaaata aaggttccaa cagctgtttt taaagaacgg
    31141 tcaacaatag caacgacctt agcttcagcc gctgtgcctt tgagacggtc tgctggcttt
    31201 ttaaccacta cttcgacagt atcaccatca atagcatacc caacaccatt acgaccgata
    31261 aacatatcgt cttcattctc atccacatgt aagaaaccaa agcctgcttt attggcacga
    31321 aaaacaccct gaacagtagg ttcttttttc ttttcacgct cttttctaag agccagactg
    31381 ccatcatctg aaaaccgtaa caggctctga ctttccattt tggaaatggt tttaataagg
    31441 ctgggaaaat gcttagctcc agccatctcc aaggccgcag ccaagtcgtt cacattggac
    31501 ttgccatgct ccttgaggta agtcataatt ctttctttca tcatacctaa tttctatttc
    31561 tgttttgttt gttggaggat atcaatccgt ttcgctgata aaaaaatgag ctagactaac
    31621 tctgtcaagc tcactcctta tttacttgat aaaatggcaa gcacaagtgc aatcgctagc
    31681 caaaaaaata ctagaactgc tgtgaatcgt tgcatgaatg cttcaaagcc acgagctttt
    31741 gtacgttcaa acaaggcttc tgaaccgctg ctatcaaaaa cattgctact tgggtttttt
    31801 tgaggttgca taaaaatagc aatcacaagc aataccgaca gtactagcaa tatcgtaagt
    31861 agtaagttgt acatgttatt tcctccacta ttgtcatgac cattctaaca caattcttat
    31921 caagtttcaa catgcagggt tacttttctc tccttggggc agtatttggg aacttttatt
    31981 ttttccggat gactgctttt atttttactg gttagataat tgttactgcc acactcactg
    32041 cattctaaat taatttttac tctcacacaa tatccttaac tttaatatat tttcgaaata
    32101 taatcaaact ccatattaaa ttaacaaaca caatcaaact agtcacataa aaaacagaac
    32161 ggtagcctag cactacagcc acatttgatc cgataaaagg gccaatgact tgaccaaggt
    32221 tactaaacat ttgattatag gcaaatactc tagaaatacc ttcttttggt gttaacttag
    32281 ttagtaaaga gttaatactt ggcatcaaag cgcccacacc aaacccataa gcaaagcgta
    32341 gtactcctaa ttgaaaactg gtctgcgcta aggcactgct aaaatacatg ataaagctat
    32401 aacacaaagc ggctaacaat aaccgatgat tgccaaaacg atctcctaat tttcctaagt
    32461 aagaactgct aaataagcta gaaaacccca tggccgaaac taccaggcct gaagtaaaca
    32521 ttaagttgtg agtttgacca agatgacgaa tatacaatga taaaatgggg gccactgatt
    32581 gagctgaaat ttgaataatc atactagtca caaacaaacc aagcataatc tggggacttt
    32641 ttacctgttt taaaatgact tttgtaggta tcatctcaaa gcgcctaatc ggcttaaact
    32701 cttctttaac ataaacagct gtcattaaag aacataaaaa taatatcaca ccaactaata
    32761 agaaaacttg tcgaattcct aatagctctg ccaaaatccc ccctagtaaa ggaccaatta
    32821 aagcgcctgc tgtcactcct gttgctaatg tgcctaaagc ataacctgac tcctcttttg
    32881 gggcttggct ggcaatcaag gcagtggcat ttggcacata gcccgcaaag acacctgtta
    32941 ataatctcaa aatgagtaac caaaacacat taggaataat agctaaacca cccatggtaa
    33001 aggtcatgac aaaactggct cgcagcatca taggttttcg gccataacga tcagccaact
    33061 tcccccaaac aggagcaaat aaagctgaag caagtgccgt cacagcaacc gccagaccag
    33121 catagtattc tactaattct gtcggagtac ctaaattttc cacatataga gccataaaag
    33181 gcataacaag tgaaaagctt gccccagtaa agaagttacc aagccaagcc acttttagat
    33241 tttgtcgcca gttcactgtc gttaccgctt tatcactaat agatcatcat cttctttcca
    33301 ttgggccttt gattattata acacatttct gccaaaaaca gtgtcccata atagtgctaa
    33361 gtctcatctg atagccttca tcgtttacgt aaactaccca gtaaacctct gaccagttct
    33421 cgcccaattg ttctaaccgt cgtactaata aacgcatctg ttgctttttc gacgactgtt
    33481 tttcgtggac gcccaacaga acgccgatta gcttttttgg tagctgcttg cgccttgatg
    33541 gcttctttag cttctttttc gcgttgtgct gtcgctatcg cttcttgggc cagtcggttg
    33601 tcttctaaaa ccttagcagc taatttttca taagcagatt cacggtcaat ggactgacta
    33661 tatttactgg caaaaggtga ggactgcaca agctgctgac tctctatctc tgacaaaaca
    33721 gcaaagctac tcttaggcgg catgatatag gcacgttcaa cgatggatgg ttgtccctta
    33781 tcattcaaaa cagaaatcaa ggcctcaccg acttctagtt ctgtaatcac ccttgccaca
    33841 tctaagtcag gattttgtcg gaaagtatcc gcagcgacac gaaccgcctt ttgttctttc
    33901 ggtgtataag cgcgcaaggc atgctgaata cgatttccaa gctgagccaa aaccgtttct
    33961 ggcaaatcaa gtgggctttg agtcacaaag aaaatcccaa ctccctttga acgaataagt
    34021 cgcacaatct gttcaacttt ttccaagaaa actttagggg catctttgaa gagcaagtga
    34081 gcttcatcaa aaaagaaaac catcttaggt ttgtccaaat ccccaacttc tggcaataat
    34141 tcgtacaact cagacaagag ccaaagtaag aaggttgtgt aaagggtagg agattgaaaa
    34201 agcttagtcg ctgacaaaat gttaatggct ccatagcccg aagcaacatc taattgcatc
    34261 aaatcagcca catctaaagc aggctctcca aaaaattggt gggctccttc ttgttctaat
    34321 gtcaagagac ttctttggat agccccgata gattgcttgg cgatattacc gtaatggctg
    34381 gagtagtctg aggcatggtc accaacttct tttaagatag cctgcaagtc tttcaaatca
    34441 atcaaaagcc agcctttctc gtcggcgatt ttaaagacga tgtttaagac acctgtctgg
    34501 gtatcattaa gattcaataa acgcgacaac atcatcggtc ccatttcaga aatcgttgtc
    34561 cgtaagggtt gaccgttttg accaaaaaca tcccacattc ttacaggaaa ggcctgaggc
    34621 tggtaatcac ttaccccgat tgtggctaga cgcgcagcca atttatcagt cacctctcct
    34681 gctttagtaa gattagacag gtctcctttg atatctgcca aaaagactgg cacaccagct
    34741 aaacttaatt gctctgctag cacttttaag gtcacagttt tacctgttcc agtcgctcca
    34801 gcaatcaaac cgtgacgatt tagcatccct aattgtaagg ctatcggttg attgcctttt
    34861 gcaattgtca ttgtagtcat aggcttcctc ttttttgatg atcatacttg tcgatatgtg
    34921 tttacccata agttaaagtg tttagaaggg tctcgctctt aatactatta taacatataa
    34981 cccttgatgc gctaatttaa aagggaccag ctagttttag acagaaatag cttgattttg
    35041 acagctatga tacgaatctc tcagcttagc tacctcggat tggctaatga caacaacgca
    35101 gattgtactt gctttattaa cgtttccatg tctccattat tatcaatcac tagactggca
    35161 tagggctttt tttcctctat tggtaactga ctggctattc gctgccttgc ttttcctttg
    35221 tctaaacgat ttctagccat taagcgctgc aattgtgtct gagcatctac ataaaccagc
    35281 caaatcgcat caaaccagtc ttggtagccc aattccatta aaaggggaat atccatgaaa
    35341 aaaatggctt ggctctgtgc taagtggtca cgcttagccg ccaattcttc cttaatgatt
    35401 tgattttgaa tggctgagga tgttgccata ttatctgggt ttgaaaaaag catttctgac
    35461 agctttgtgc gatctaactc accatctgct tttaaaatct gattcccaaa agcttctcta
    35521 agagcctcgt aaaggcgacc gcctttttct tgcaagtcat ggaccacttg atccgcatca
    35581 atcacctgat aaccagcttt tctaatgacc ttaaccacag tcgattttcc tgatgcaatg
    35641 cctcctgtga tgccaataat catggtcttt ttttctggca ttttggacaa atgtgcgtcc
    35701 cccttcctcc aacttttaat ttgacaatag cctgtccaca acgaggacac ggtttcccag
    35761 tttgaccata aacctgcaaa tagtcctgca tggtgccatc agctcccaaa gcattacggt
    35821 aagtacggac ggtcgaaccg cccttttcaa ttcctaaagc taggattcgt atggtttcat
    35881 catgcagccg ttttatctcc gccttattca acctagaaga cgcagtttct ggatgaacct
    35941 tagcagccca aagtacctcg tccacataaa tattccctaa accagcaacc aaggtctggt
    36001 ctaacagatg tggttttatg ggtttttggg aggatagcag agcagcctca aaggttttta
    36061 acttaaaggt ctctttcttt ggttcagggc ctagttttcg tttggcaaaa aacgctgata
    36121 attgagattt tgttatcaaa tcaaaggttc caaatttacg cacatcttgg taaaccaagg
    36181 tcgaaccatt tttcaattca aaaaagacat gaaagtgttt gttatcaggg acctcatctg
    36241 gaaaaagcaa atatttgcct tccatccgca aatgtgatac caacactaat tgccccaaat
    36301 caattaataa atattttccc cgacggccaa ctgactgaat gatttgacca ggtagggtca
    36361 gggcaaacgt ctctaagtcg gttttaacca tttttggcac tttaagcgta acagccacaa
    36421 tttcttggcc aagcactaaa gtctcaagcc ctcgtcggac cgtttcaact tctggtaatt
    36481 ctggcataat ccgttattta aaggacagct agaccctttg ctgctacaaa gaataaactc
    36541 agcataaaaa agtcactcaa tagactcagg tctaggatac tgactgcgtt tcaaaaaaca
    36601 cagctgtcct tctcctttct tatttcttat tttacgaaca catctgatag aaagcaactc
    36661 ataaatctgc ctcagaccag tcctcatccc ccttacctcc cagcactatt tggcggtaat
    36721 gttccttata actataatac aatgagtcct ctccaagata cttaaaaatc tgcaaggcct
    36781 gccgcatgtc ctcttctcca gacatttctt cctgctttaa tttataatac ccatgcacat
    36841 ataagaaaac agtcttttca taaaaattga gctcgtctcg cagcaaaaga tcaattttgt
    36901 taatcaaata gcgactatgt tcaaagcaag aatggtcaat actgataatg agacaattaa
    36961 tggataactg agtcactaac attttattgg ttttattttg ttccgaataa gcaaaagatg
    37021 ctaccatttc cttagtcaat aaaaataggg tattgtaatt catgaagcgc gagcaattcc
    37081 ccagaagaat gatttcataa tatccccact gttccacttt aaataaataa tctgtgaggc
    37141 gagtcaattc ttcttgacta ggtgcaattg aggagtcaat agaaaaaaga atagctttaa
    37201 tcatcgttcg ttcataatct ttatgggcat aatccttcaa cagtttggtc aatttaacca
    37261 cgtttttctc agcataacat tttctcgcct gacttaagag cgtgaaaaaa tgcgtatgcg
    37321 tcttggaatg agccgaaaca aattcgtcaa tcgtaatatt cagtttatcc aaaagattaa
    37381 gcaagcgact gcaggtgatt tctgattccc ctcgttcaaa tcttgaaatt tgtgacttgg
    37441 acaggtattc atctgctaaa aaagaaatgc taacctgctt acccttacgt aatcgcctta
    37501 aggtttttcc gagttctttt tccatttttc ccactttcac aacaaaaaat taaaatctgc
    37561 taaatccatt atataataca agtacaaaat agaaaatcag aaaggagaca gtcatgaaaa
    37621 aagttaataa agctttgctt tttacattga tcatggatat cctgattatc gttggtggat
    37681 aacctttcgc ttaaaaacaa tccacttttg ggaaaccctt atatttagtt tgtaacagga
    37741 aaactaatag atattaaaaa gagtgttagc gacctagcgg cagctttact cttaagtgcg
    37801 tttgctgcca ttgacagcaa ggtcaaagct gatgaaacag caggggcata ccgttatgat
    37861 gtgtctaaac aaaacactta ctatcaaact atcagctcca ttaaccagca acgacctggt
    37921 gccactctcg acgaatgagg tgattattat ggaaccctta cttcatttac ttcccaaagt
    37981 cgtaccagta cttatacact tggtagacaa agccaacgta tcatggttaa cagtgatatt
    38041 acagttacct atactcttaa agacaatttg gtagcccttc ataaggaata taaaaccttt
    38101 gtcaaaaaaa tattacagat aaaccgatca ccagtgacta cgacaaggct aaggttattt
    38161 ataaccattt aatcaacgaa tacacttacg ccacaaaata acttagcacc aatcgcaaaa
    38221 ccgtaagcag catcaacaat catactccta aagctcttta tcgtagcaag tgtggtgttt
    38281 gtcaagctta tgccgtcatg tttaaagata tggcagaagc tgcaggctta tcagcttggt
    38341 atgtgataaa aaaggattga atgagcacca tgcttgaaac atcgttacca ttcatggtgt
    38401 taattcctac gttgatgcta cctggaatga ttcagcatac accacaaggt atttccttgc
    38461 tggaaaagac gtgaggaata ctgagtaccg tttagatagc cagtacaacc acttggctaa
    38521 tgctatttta aatatcagct actaaaaagg ctaaactaac aacaactaca tttttcaact
    38581 agaaaggcct aaacaatgac caaaaaacag ttttatctta tcttagctat tagcatactg
    38641 tgttttattc ttgccgtcag ccctaaacca ccaattccta aacacactat tgaaaggaaa
    38701 aggactgcga tgaccatagg ccactcaaaa caaaaccctc acaaaaaaga agcccttaaa
    38761 cacaaaaaaa ataaaaataa ggctctattt cttccaagtg gggacagcgc tatcaaatct
    38821 gttattagca tgacagttag actcctgtta gatgaccaat cttagctctc ctaaaatcag
    38881 tggacactac catttcggta gtgttctttt ttaagctaat ctcatcacca cgctttcttc
    38941 aagctaaaaa actgatcatg aaagagcaac cccatcaccc taacagccca ctttgcccgc
    39001 actttcttaa aaagttagga gaaaacgata taccaattat tttctgatcc cagctcccta
    39061 tcattaacaa gatgtgtagc acggacctac atacaggtaa gctagagtca ttctcgctat
    39121 ttcggacaaa aagaggccgg tagttccctg acctcttctt tatttatctt gtaacactcc
    39181 tcttcttagc gagacttact agcacataat tcttaagcac tcttatcatt tagactagga
    39241 agaacaccat ctgaatgaag caaatgatgc gtcagatctg ccagagtatc atctccataa
    39301 gtaatccctg tttggtgctg tttctgatga aaatcagatg actccttaac tttagcagtc
    39361 acctgctggt cacttggatg aaaattaatt tctctcttgg cttcagccgc aactggctcg
    39421 aggaacaact tattgacatt acggtcttcc tccaaatgat taaggatagc agtatcatct
    39481 tctaacgaaa gaaccttctt gggtaacaaa aaggggttat ttagccttgg ctcagtaacg
    39541 acttccttgg ctactcgtga tagtttatta ttgtcttcct caggtatcaa acggttaggc
    39601 aatcgatgca attcttccgg ctatgaggcg ctcacagcta cttcagaaga aacctccaag
    39661 ccctttcctg tttgcctgtc taaagcaacc ggcaaattat taactaactt tttaacctgt
    39721 cctcagcgct catttcaaca ctaggaagag ctgtcgctgg ttctagccag ctaccaactt
    39781 ctgctggtct taccttaaca tcgctggcag gcttctcctt ctcatcttca ttaggtctaa
    39841 aaggctcaaa ccgccctgtt gatttttcat ctatgacctt tatattgtct tttactacat
    39901 gagacagtgg ttcgacattt accatagctt catgctcgtt aaggctctgg tctcgtttga
    39961 atttaccagg aataccagga gtcggtgttc cgacagattg atcggcttca gtaggctctt
    40021 ttacggagcc ttttttccat aaaactgctg gaggaataat atccgtagag ccagccccat
    40081 aagtaatcgt ggccagcccc cccccctttg gattaataga tactttaata ttgtccggta
    40141 aggctccact acctcctgtg gttaaaaggg cacccgcatt tttaatgaga gttgtcattc
    40201 gttcaatacc agcaacgcca atatggttca agtcatctac tggtaactga tcaacagatt
    40261 tcatggtgta tttccaagct tgcggttgaa caagaaaaac agttccttta tacattgtgt
    40321 tatgcatatc atcaatgact ttgaaatacc ttgtatattt ggtttgtaca ttagggttag
    40381 caacgggaac agtgctttca ggaactcctg acatcgtaaa aatctctcta gcagaagttt
    40441 gaaagccctc ataaacatga cccataccag gaacagtctg agcggtcaaa gacttaccag
    40501 aaccatccaa agcaatgcga atatctttca tcagatacat ctgatcggta taggtaaacc
    40561 taaccgtgtt agcctcacct cgatacaaaa ccacttggga gggctgatcc gaaaaaaact
    40621 gagaggttgt ttcttcctta tagccaactc caaaacgtct atcagacgaa tggactgaat
    40681 ctgccgcaac tgtactactc aaactgacta gtaaagtagc cgcacaacca actaataaac
    40741 tgctcgtttt aactttaaca tctcttaaca tcatcatgtt cctttcatta ttttttattt
    40801 aggaattcgt catactatat tttctcatat tagtctaaca aaaaactcgt aattctatat
    40861 aaatcatata aaatgacgag ctttggcaaa gatgagtata gcaggatgga aaacagattg
    40921 tttctgaaac ttcaaagg
//
LOCUS       DAUGFP010000013        38406 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.013, whole genome shotgun sequence.
ACCESSION   DAUGFP010000013 DAUGFP010000000
VERSION     DAUGFP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38406
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.013"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..90
                     /locus_tag="VH663_000687"
     CDS             <1..90
                     /locus_tag="VH663_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8173907.1"
                     /translation="FNLVEGLIFAITFYFVYIASKPILERYLH"
     gene            92..745
                     /locus_tag="VH663_000688"
     CDS             92..745
                     /locus_tag="VH663_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ8173908.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILLIGLSRIYLGVHYPSDILAGFVLGFGILHFIYPFYKQKRFEWRFLLKQD"
     gene            1038..1958
                     /locus_tag="VH663_000689"
     CDS             1038..1958
                     /locus_tag="VH663_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEQ8173909.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            1997..2551
                     /locus_tag="VH663_000690"
     CDS             1997..2551
                     /locus_tag="VH663_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ8173910.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLSNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADV"
     gene            2684..4018
                     /locus_tag="VH663_000691"
     CDS             2684..4018
                     /locus_tag="VH663_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEQ8173911.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQDQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            4096..4887
                     /gene="pflA"
                     /locus_tag="VH663_000692"
     CDS             4096..4887
                     /gene="pflA"
                     /locus_tag="VH663_000692"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEQ8173912.1"
                     /translation="MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            5018..5953
                     /locus_tag="VH663_000693"
     CDS             5018..5953
                     /locus_tag="VH663_000693"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEQ8173913.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            6029..6682
                     /locus_tag="VH663_000694"
     CDS             6029..6682
                     /locus_tag="VH663_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEQ8173914.1"
                     /translation="MITIFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNHVMIKEKTNITRSQLTLANYFYRLKRGEKPSAEQIDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(6727..7764)
                     /locus_tag="VH663_000695"
     CDS             complement(6727..7764)
                     /locus_tag="VH663_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ8173915.1"
                     /translation="MLFMVNKKRRPLLKSQRHLLRLYTSLILLLVSLMGLALSLGESH
                     LSFLDLVHVFLGKSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAVVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTMLSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGSNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(7725..8777)
                     /locus_tag="VH663_000696"
     CDS             complement(7725..8777)
                     /locus_tag="VH663_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ8173916.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKSYHQLRLVLAGVMVSILLSALGQGITNYYHLTNAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYFLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(8767..9699)
                     /locus_tag="VH663_000697"
     CDS             complement(8767..9699)
                     /locus_tag="VH663_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ8173917.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            complement(9725..10507)
                     /locus_tag="VH663_000698"
     CDS             complement(9725..10507)
                     /locus_tag="VH663_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173918.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAIQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(10753..12198)
                     /locus_tag="VH663_000699"
     CDS             complement(10753..12198)
                     /locus_tag="VH663_000699"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEQ8173919.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGLSFERLSYDSRQ
                     VDGKTLFFAKGATFKAEYLKEAIAKGLPLYVSEVDYKLDIPVVLVTDIKKAMSLIAMA
                     FYDNPQDQLKLLGFTGTKGKTTAAYFAYHILKESYKPALLSTMNTTLDGQTFFKSQLT
                     TPESLDLFAMMAECVKNGMTHLIMEVSSQAYLVGRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIAKHYL"
     gene            12286..13920
                     /locus_tag="VH663_000700"
     CDS             12286..13920
                     /locus_tag="VH663_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEQ8173920.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGVVFSAIMYLGSPLFTSLSGGDAKLIPIMHSLSLAVLVFPVMSVIRGIFQGHNN
                     IKPYAISQIAEQLIRVIWMLLTTFLIMKLGSGDYASAVTQSTFAAFIGMVASMGVLGY
                     YLWKQGLLTAIFSKPDHTVSIDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWSFVNT
                     MSLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLLALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYAFKPTGRLTSLLYLLII
                     GGLGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            14088..14717
                     /gene="upp"
                     /locus_tag="VH663_000701"
     CDS             14088..14717
                     /gene="upp"
                     /locus_tag="VH663_000701"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEQ8173921.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEVHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            14941..15531
                     /locus_tag="VH663_000702"
     CDS             14941..15531
                     /locus_tag="VH663_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEQ8173922.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK"
     gene            16026..16301
                     /locus_tag="VH663_000703"
     CDS             16026..16301
                     /locus_tag="VH663_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEQ8173923.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIIYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKG"
     gene            16550..17185
                     /locus_tag="VH663_000704"
     CDS             16550..17185
                     /locus_tag="VH663_000704"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEQ8173924.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLTKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            17203..18078
                     /locus_tag="VH663_000705"
     CDS             17203..18078
                     /locus_tag="VH663_000705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEQ8173925.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHTNAANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            18097..18337
                     /locus_tag="VH663_000706"
                     /pseudo
     CDS             18097..18337
                     /locus_tag="VH663_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tpl protein"
     gene            18741..19064
                     /gene="yabA"
                     /locus_tag="VH663_000707"
     CDS             18741..19064
                     /gene="yabA"
                     /locus_tag="VH663_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEQ8173926.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            19069..19932
                     /gene="rsmI"
                     /locus_tag="VH663_000708"
     CDS             19069..19932
                     /gene="rsmI"
                     /locus_tag="VH663_000708"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEQ8173927.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGAISEILSYL
                     EETPLKGECLLIVAGAQVDSEVELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            19959..20351
                     /locus_tag="VH663_000709"
     CDS             19959..20351
                     /locus_tag="VH663_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8173928.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(20398..21027)
                     /locus_tag="VH663_000710"
     CDS             complement(20398..21027)
                     /locus_tag="VH663_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEQ8173929.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTTENQ
                     LDTDAIEQLLPATQGLPLVFHMAFDHIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQSLSQLTGTAIVHGTKII"
     gene            21327..21683
                     /locus_tag="VH663_000711"
     CDS             21327..21683
                     /locus_tag="VH663_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEQ8173930.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGK
                     VLQVGYRKPYQELDLG"
     gene            complement(21757..22584)
                     /gene="xth"
                     /locus_tag="VH663_000712"
     CDS             complement(21757..22584)
                     /gene="xth"
                     /locus_tag="VH663_000712"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEQ8173931.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDTQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHLHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYCLASNRIVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            22818..23999
                     /gene="lctO"
                     /locus_tag="VH663_000713"
     CDS             22818..23999
                     /gene="lctO"
                     /locus_tag="VH663_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEQ8173932.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDYLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVNQKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            24265..29208
                     /gene="scpC"
                     /locus_tag="VH663_000714"
     CDS             24265..29208
                     /gene="scpC"
                     /locus_tag="VH663_000714"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEQ8173933.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLVGSVFLMMTTTVAADELTTTSEPT
                     ITNHAQQQAQPLTNTELSSAESQSPDTSQVTPETNREKEQSQDLVSKPTTTELADTDA
                     APMANTGPDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFGDFD
                     EDWENFEFDADAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDETKY
                     ESHGMHVTGIVAGNSKEAAATGERFLGIAPEAQVMFMRVFANDVMGSAESLFIKAIED
                     AVALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDP
                     LATNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVD
                     FKDIKDSLGYDKSHQFAYVKESTDAGYNAQDVKGKIALIERDPNKTYDEMIALAKKHG
                     ALGVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVS
                     KAPSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIA
                     GASLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNI
                     DGAVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKDKTLRYDTELLTDHVDPQ
                     KGRFTLTSRSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRF
                     RDSQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGK
                     HFTGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNN
                     QDFAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTL
                     LGTAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDP
                     ETNRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVADNKTFVERQA
                     DGSFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKE
                     TLKDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKG
                     LKNNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQ
                     YVVTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVRE
                     EVFYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVED
                     RAGNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYY
                     NNSGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQ
                     ITAHFDHLLPEGSRVSLKTAQGQLIPLGQSLYVPKAYGKTVQEGTYEVVVSLPKGYRI
                     EGNTKVNALPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKTTTSAT
                     AKALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            29992..30699
                     /locus_tag="VH663_000715"
     CDS             29992..30699
                     /locus_tag="VH663_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEQ8173934.1"
                     /translation="MSKKKRKHYQVYEGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKGLHWYLLSSFIL
                     TGIAFGTALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALIPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
     gene            30939..32939
                     /gene="metG"
                     /locus_tag="VH663_000716"
     CDS             30939..32939
                     /gene="metG"
                     /locus_tag="VH663_000716"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEQ8173935.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
                     EVEWVSEESYFLRLSKYADCLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGT
                     AFDADLSQLIDAQLADYHKYMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVSDLSTLALAAFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVA
                     NLKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVPNGSIIG"
     gene            33433..34446
                     /gene="nrdF"
                     /locus_tag="VH663_000717"
     CDS             33433..34446
                     /gene="nrdF"
                     /locus_tag="VH663_000717"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ8173936.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRARVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            34450..34938
                     /gene="nrdI"
                     /locus_tag="VH663_000718"
     CDS             34450..34938
                     /gene="nrdI"
                     /locus_tag="VH663_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ8173937.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQTNNLITERTHPCHKPMRHT
                     SH"
     gene            34905..37085
                     /gene="nrdE"
                     /locus_tag="VH663_000719"
     CDS             34905..37085
                     /gene="nrdE"
                     /locus_tag="VH663_000719"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ8173938.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLVNPAIKK
                     TYISARKFFQFIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVEAGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDKATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTASGASDETTTECQSCMI"
     gene            complement(37291..38043)
                     /locus_tag="VH663_000720"
     CDS             complement(37291..38043)
                     /locus_tag="VH663_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEQ8173939.1"
                     /translation="MLKKRYQLAIVLLLSCFSLIWQTEGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAETLIKKRWGKGLIYPRAEQEAMAAYTCQQAGPINTSLDKAKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHTDITSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTVLKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDHKKLHIEACFKGSL"
BASE COUNT        11929 a         7576 c         7981 g        10920 t
ORIGIN      
        1 tttaatcttg ttgaggggtt gatatttgca attacctttt attttgtgta tattgcaagt
       61 aaaccaattt tagaaagata cttacactaa tatgatgaca aacaaacaaa ctcacttttt
      121 aatagcttct tttgcattgc taatctttgt tataataggc tatacggtca aattttttcc
      181 tgaacgtttg gcacttctag ataatactat tcaagcagag attagaggca accttcctat
      241 agtgctgacg caattcttta ggggtgttac tgtatttgga aatgtcatga cacaagtttt
      301 gttagttatt gtgtcggtcc tagttttatt ctttatgaag tggaaaattg aagctttgtt
      361 tatcttatca aatggtgcca ttgcagcatt tttgattacg actctcaagc tcttttacca
      421 aagaccaaga cctgctattg agcatttggt ttatgctggt ggctattcgt ttcccagtgg
      481 ccatgcgatg ggtagcatgc tgatttttgg tagcctattg attatttgtt atcagaggct
      541 acattcaaaa ctattacaat ttgtgactag catgatcttt ataatactaa ttttattaat
      601 tgggttatct cgtatttatc taggagtaca ctacccaagt gatattttag caggctttgt
      661 attaggattt ggtattttgc actttattta tcctttttat aagcaaaaac gatttgaatg
      721 gcgatttctt ttgaaacaag actaattttt cttgagaaga catagggctt atttaaatca
      781 tttagaataa cattggcaag catcacccct tgattgccag tgttatttta gttttagtta
      841 gatgatgtga gctgataatt agtaaatacg tttagataaa aaacgattaa agtgctatgt
      901 aacgtcagaa aagacatgat ttttgttgat ctgtaatcat taagtaagct gatgtagaag
      961 tagtgaattc taatgactga gctatctttt gcatagatga ctatcttgct agtaatgatt
     1021 tgtgctacaa tagaaacatg aaaaaaagat atcagacatt aaacgagcat taccgtcaac
     1081 tatttggcgc aaaaatgttt aaagttccga ttgatgccgg ctttgattgt cccaatcgtg
     1141 atggaacggt agctcatgga ggatgtactt tttgcacagt atcaggttct ggagatgcta
     1201 ttgtagcacc agatgctcca attaaagagc aattttataa agaaattgat tttatgcacc
     1261 gtaaatggcc tgatgtcaat cgctatttgg tttattttca aaactttacc aatactcatg
     1321 atacagttga tgtgattcgc gaccgttacg aacaagctat taacgaacct ggagttgttg
     1381 gtattaatat tggcacacgg cctgactgtc taccagatga taccattgct tacttagcag
     1441 aattatcaga acgcatgcat gtgacagtgg agctaggctt acagacaact tatgaagaaa
     1501 cttctaggct aattaacaga gcacattctt atgatttgta taaagaaacg gtgaggcgac
     1561 taaggcatta ccccaacatt aatattgtct cgcacttgat taatggattg ccaaaagaaa
     1621 ctcatgacat gatgcttgaa aatgtgagac gctgtgtaac agataatgac attcaaggaa
     1681 ttaaattgca tttgctgcat ttgatgacca atactagaat gcaaagagat tatcatgaag
     1741 gacgcttaaa gttgttgagt caaaaggact atgtttcgat tatttgtgac cagctagaga
     1801 ttatccctaa gcatattgtg attcatcgta ttacaggaga tgccccacga gatatgttga
     1861 ttgggcccat gtggagtcta aataaatggg aagtattgaa tgccattgat aaagaaatgg
     1921 aaagacgagg aagctttcag ggctgtaaag ttgactaatt tgtctcattt tgacggattt
     1981 ggataggaga aaagtgatgc taaaacgccc cattcatctc tcgcatgatt ttttagcgga
     2041 ggttgtggat aagtctagtg tggttgttga tgccacgatg ggtaatggaa atgatactgc
     2101 ctttttggca caattagcta aaaaggttta tgcttttgat gtgcaagaac aagctattag
     2161 aaaaacaagt gagcggttag cgcagctagg attatcgaac gctgagctga ttttagcggg
     2221 tcatgaagcg gttgatcagt atgtgacaga gccggtgcgc gcagcgattt ttaaccttgg
     2281 ttatttgccc agcgcagata aatctattat taccttacca aataccacac tccaagcttt
     2341 atcaaaacta ctaacgttat taatggtagg tgggcgtatt gctattatgg tctattatgg
     2401 gcatgatgga ggcagtttgg aaaaagatgc tttattagat tttgtcaagc aattagacca
     2461 aagaaaggtt tctgcaatgc tttatcagcc tcttaatcag gtgaataccc cacctttttt
     2521 aatcatgttg gagaaattag cagacgttta gctcttatcc ctgtgtcaag tctactagaa
     2581 gttctgcgat tatttcggtg gaaaaagaag aaatcccata ggctttggct ataaatgtga
     2641 tataatgaag tcactatatt atttttttag gagttacatt aacatggaag accctgtgag
     2701 tcagccctta gtgattcaat ttttattgtt agttgtttta accttgttaa atgctttttt
     2761 ttcagccagt gaaatggcct tagtttctct caatcgttct cgggtggaac aaaaagcagc
     2821 agacggtgat aaaaaatacg ctcgtttgtt gcgggtttta gaggaaccta atcatttttt
     2881 atcaacgatt caagttggga ttacctttat tagtttacta tcaggagcaa gtttatcagc
     2941 ttctttgggt aaggtgatct caggttggct aggtaattca gcgaccgcaa ggacagctgg
     3001 tactatcatc tccttggttt tcttgactta tgtctctatt gttttaggag aattgtatcc
     3061 aaaacggatt gccatgaacc tcaaagacaa gttggcgatt gtttcagccc ctattatcat
     3121 tgggttaggg agactggtta gtccctttgt atggctctta tcagcttcta ctaatttgct
     3181 gagccgactt acccctatga cctttgatga tgcagatgag caaatgacac gtgatgaaat
     3241 cgagtatatg ttatcaaaaa gtgaggcgac ccttgatgct gaagaaattg agatgttgca
     3301 aggagttttc tcacttgatg aaatgatggc gcgtgaagtc atggtcccaa ggaccgatgc
     3361 tttcatgatt gacattaacg atgatccgct tgaaaatatt caggaaatct taaaacaaag
     3421 tttttcacgc attcctgttt atgatgtgga taaagataaa attatcggtc tcatccacac
     3481 taagcgtctc ttggagtcag gtttccgcca gggatttgat cagattaaca tgcgaaaaat
     3541 gttacaagaa cctctttttg ttcccgaaac catttttgta gatgatctct tacgccagct
     3601 gcgcaatacc caaaatcaga tggctatttt gctagatgaa tatggtggtg tggcaggact
     3661 tgtgactttg gaagacttgc ttgaagaaat cgtcggtgaa atcgatgatg aaaccgataa
     3721 agcagaacaa tttgttcatg agattggaga caatacctat attgttgttg gtaccatgac
     3781 tttaaatgag tttaatgact attttgatac cgaactagaa tcagatgatg tagataccat
     3841 tgctggtttt tatttgacag gtatcggaac cattccaagc caggatcaaa aagaagccta
     3901 cgaaatagat aacaaagaca aacatttagt tctaatcaac gataaagtca aagatggccg
     3961 tattacgaaa ttaaaattaa tcctgtctaa tatagaacag attattgagg aagactaggc
     4021 tccatgccta gtctttttga tgaaaagtgt tataatgaaa atgtaaaaac gtttacaagt
     4081 aatgaaggag atgcaatgac tgagaaagat tatggacaag taacagggat ggtgcattcg
     4141 acagaaagtt ttggttctgt agatggtcca ggcattcgtt ttatcatttt cttgcaaggc
     4201 tgtaaattac gttgccaata ttgccataat ccggatactt gggagatgga aaccaacaat
     4261 tcaaaaatca gaacagtcaa tgatgtcttg aaagaagccc ttcagtacaa acatttctgg
     4321 ggaaaaaaag gaggaattac cgtatctggt ggtgaagcta tgcttcaaat tgattttatc
     4381 actgccttat tcatcgaagc taaaaaatta ggtatccata ccacactaga cacctgtggc
     4441 tttacctata gaccgacacc tgaataccat caggttcttg ataacttatt ggcagtgacc
     4501 gatttgattt tattggactt gaaagaaatt gatgaaaaac aacataaaat tgtcacgcgt
     4561 cagcctaata aaaacatatt gcaatttgct aggtatttat ccgacaagca gattcctgtc
     4621 tggattcgac atgtcttggt tccaggcttg actgatattg atgatcattt gacacgattg
     4681 ggtgagtttg tcaaaacctt gaaaaatgtc gataaatttg aagttttgcc ttaccatacc
     4741 atgggagaat tcaagtggcg tgagctaggt attccttatc agttagaagg tgttaaacca
     4801 ccaactaagg aacgcgttca aaatgctaaa aatctgatgc agaccgaatc ttacacagaa
     4861 tatatgaatc ggattcatca gtcataagag ctgttatagg gttgaggttt cctcaacttt
     4921 ttaaagtatt ataagcagat cttttgtaat cttcctttga ttttaggggg taattttgct
     4981 aaaataaaat gaataaacaa aatgaggtag aatgattatg tcaaaaattt tagtttttgg
     5041 tcatcaaaat cctgatacgg atgctattgc atcatcttat gcttttgatt acttatccca
     5101 aaaagcgttt ggtttggata ctgaagtcgt agctttagga acacctaacg aagaaacagc
     5161 ttttgccctt gattattttg gtgttgaggc accacgtgtg gtagagtctg ctaaagcaca
     5221 agggtctgaa caagtgattt tgacagacca caatgaattc caacaatcca ttgcagatat
     5281 ccgtgaggtt gaggtatatg gtgtggttga tcaccaccgt gtggctaatt ttgaaactgc
     5341 aaatccttta tacatgcgtg tggaaccagt aggatcagcc tcgtctattg tttaccgtat
     5401 gtttaaagaa aatggcattg aggtgccaaa agcgattgca gggatgctct tatctggttt
     5461 gatttcagat accttgttgt tgaaatctcc aacaactcat gtgtctgatc accttgtcgc
     5521 tgaagaattg gcagagctag cagaagtgaa cttggaagac tatggaatgg ctcttttaaa
     5581 agcaggtaca aaccttgcaa gcaaatcaga agtagaattg attggtattg atgccaaaac
     5641 atttgaatta aatggcaatg ctgtacgcgt ggcacaagtg aacactgttg acattgcaga
     5701 agtcttagaa cgtcaagaag ctattgaagc agccattaaa gatgccatgg ctgcagaagg
     5761 ttattcagac tttgttttga tgatcacgga tattgttaac tcaaactctg aaattctagc
     5821 tattggagca aacatggaca aggttgaagc agcctttaac ttcacccttg acaacaatca
     5881 tgccttctta gcaggtgccg tttctcgtaa aaaacaagtg gttccacaat tgacagaaag
     5941 ttttggagct taatgaaaag acgctttaaa aggacttggt ttttttgacc aagtcctttt
     6001 ttcactcttc tatgctataa tagaaggtat gataacaata tttcattcag ataaactcac
     6061 cagacaacct ttctttcaag accttatcaa ttatctcgac cagcatgatc acgttatatt
     6121 acgagagatc aaaaaagcct ttcctaatgt gacaggtatt gacaaggcca tcgaaagcta
     6181 tgttcaagct ggctatattc gccgtgaaaa taagcgttat ggcatcaacc ttcctttggt
     6241 gagttctgat cagcagctgg ccttagacac tatgcttttt gtggacacct gttcagctat
     6301 gtatgaaaat attttagcgg ttgtttttga gactcagcta acaaaccaaa ccaatcacgt
     6361 gatgatcaag gaaaagacca acatcacgag aagtcagttg actctagcta attattttta
     6421 ccgtctcaaa agaggtgaga agccatcagc tgagcagata gacttgtatg acctcttggg
     6481 ggatgtgaat caggaatatg cccttaaata tatgacaact tttttgctta aattcacgcg
     6541 caaagacctt gtgatgcaaa aacgtcctga tatatttgtg gaagctctgg ttacacttgg
     6601 ttacctaaaa caagtggagc ctacaacata ccagctgcta atgaccttgg ataaagaaag
     6661 tttaaccttt atagcaccat agaaaagagc taggaacaag gactgttctt agctttttga
     6721 gtgcttttat tttgtttttg ccattaagta aagaaaataa ggggctccga taagcgacac
     6781 taaaatccct gtctggatat tactgccaac cagatagact cgtcctactg tatctgcaac
     6841 aagaagtagg atcattccga ttaaaatact aatgggtatt gttattttat gattactccc
     6901 taccaagtat gtactaaagt gtccagctat taagccgata aaactaatat taccaaccaa
     6961 aagaacactc aaagcaccta agccagctgc taagacaagt atcaaacgac gcttacggtt
     7021 taaagggagt cctagtccaa tagcagtatt atcagctaat cccataatat taaggaaatg
     7081 ggcttgacta taagtcaata gccaaaaaca caataataaa ggagcgatga cactcagagt
     7141 aggccagtcg tctcctatta attggccgct aagccaattg atgaccaaat cgactttgta
     7201 gcgattaata tgaccaacaa gagcaaccat tagacttgaa agcatggtag taacagctac
     7261 accagtaaga attaaccgtg taggatcaat ttgtccttgt tttgtcaaag atagccaata
     7321 aacgctaaag gttgttacaa cagcaccaag catggcaaat aaaggcaagt aatggctgat
     7381 atgagcttgc tcaaagaaag aaaaggagac cacaatagcc aaagagatac ctgctcctat
     7441 agtaatacct aatacaccag agtcagctaa gggatttcgt gttaaacgtt gtaataatag
     7501 ccctgataaa gcaagagaac cgcctcctag acaagcagct agaattcttg gcagacgaat
     7561 gttaataacg atgaaagata tggcatggct agattttccc aaaaacacat ggacaaggtc
     7621 caaaaaagat agatgagatt ctccaaggga caaagctaat cccataagac ttacaagtag
     7681 caggatcagg ctagtatata accttaaaag gtgtctttga gatttcaata gcggcctcct
     7741 cttcttatta accataaaaa gcatggaaaa ccaattaagc taaccaatgc cccaagaggg
     7801 gtttcataag gcggattgag attacgacaa gcaatatcaa cccataccat gaaactagcg
     7861 ccagaaactg cacaaagcgg caaaaagtat cggtaatgat gaggtgtaaa gtgtttcata
     7921 agatgaggaa tgactaagcc gataaaagaa atgcttccag caatggctac agctgctgat
     7981 gataaaataa gaaccaaaat cataaagact gcactgatca agttggtctt ttgcccaaga
     8041 gcttttgcct gagactcact tagactaagg acggtcaggt gataagataa tagctgggct
     8101 aaacaaagac taagaataat aagaggagca atatagccga tcatttgcca attgaccccg
     8161 acaagacctc ctgcttgcca gccgataaca gcatttgtca gatgatagta attggtaata
     8221 ccttgaccta atgctgatag cagtatggaa accataactc ctgctaagac aaggcggagc
     8281 tgatggtagc ttttgccaga ttgataagaa aggccaaaga ctaaggttgc tgccaggcta
     8341 gaccctaaca acgaaagcag aataatgagg gaatagtgca agtgaggtac aaacgcataa
     8401 gccagtacca aggctagtcc tgctccagca ttaatgccta aaaggccagg ctcagcgata
     8461 ggattacgtg tgattgcttg catgatagtt ccagaaacag ctaacgcaga gccagttaag
     8521 gtagcaccaa aaagtctcgg taatcgtata gcgagtaaaa cattggcttt atggccgttt
     8581 tgctttccaa ataaaataga gttaagatcc tgatgtgata aagcaacggc tccaaagcgc
     8641 agatgacagt aaatagcaac acataagcta aaactaagta gcacaaagac aagccaaaag
     8701 atatgtgact tagtgattat ctgcgttttt tttaagcaaa aacgctcgag acacaaaaag
     8761 ggatgcttag ttttcacttg ataagattgc ctctcgtaaa gtctctaatt gatattctaa
     8821 agaaagagga tcagtaaagt aaaagacatt ggcattgact ttgatcacat ggtgtttttt
     8881 cacagctgga atggattgcc atagcttgct ttcatataga gcagaaccag tcttatcatc
     8941 ttctgcagca acgacgacat aatcaccaat atagtctggc aagacttcct gcgacaatga
     9001 caggtaacct tgtttaaaaa cttcagtctt cactttttca ggagcatcat aatggaaagc
     9061 ttgatggata acctctccgc cacgtcccca gtctttacca aaaaggtaaa cgtctttttc
     9121 ataaagcccc ataatagtaa aggttgcttt gtcacctgta acggccttga cttctttttc
     9181 ataagctgct gttttagttt tccaatcttt taaccatttc ttggctttgc cttctttgtt
     9241 aaagatgcgg ccgaagtcag ataacacttg taaataatca cgtttgcggt attcaatcga
     9301 aataacaggc gcaatttctg ccaattgttt aatattttct tctgtagagc caacaacaat
     9361 gaggtcaggc ttaagcgttg taatggcttc taaatcagtt gcagcaactt gctttgcttt
     9421 ttttacagtc tttgctaaga tggggttttt cttgtcataa gaagtaaccc cgactagatt
     9481 catatctaat tttttgaggt aaccagtgta agttgaagcc agactgacta ctcgcttagg
     9541 ctgtttagga atatctccgt aataagtcac accagcaatt tgaggcatag gcgataaact
     9601 ttttgaggcg gtgttagaat gattagttgc ttgattacca caggcgatta aggtaatagt
     9661 tgtcaaacag agtgttaata gtagagttaa ttttttcatg tatgctaatt ctcctagaat
     9721 aaagttattg aagctgataa gtcagcacta taggacagtt gtggatagga tcatcaacca
     9781 gtaccggttt gatttggaag atgtcttgaa tgataggtga agtcataaca tctgctatag
     9841 taccagtata atggatcttg ccatgtttca tagcaatcag atgatccgaa tagcgggctg
     9901 aaagattgag gtcgtgaaga accataacaa tagtcttgcc ggcatcctta tttagggatt
     9961 taacgagctc taggatttct agttgatgat ttaaatctaa ataagtagtt ggctcatcta
    10021 aaaaaatagt atcagtgcct tgggctaatg ccattgcaag ccaaaccctt tgacgttgcc
    10081 ccccagaaag agcatcaaca ggttgatcag cataggccat gacattggtg gcttgtatag
    10141 cccaatgaat ggcttttttg tcagctggac taagatttcc aaagtaagat tgatggggaa
    10201 aacgtccgta agaaaccaat tcgtaaacag ttatgccgtt agttgcttct tgaacttgag
    10261 gtaaaagagc tagtttttta gcaacctctt tagtttctaa tgtggcaatg ttttgcccat
    10321 ttaaatacac aactccctgt tttggtggta ataatcttgt cagtgctttt aataaactag
    10381 acttcccaca gccattggcg ccaataatgg tcgtaatttt accttcagga atataaaacg
    10441 ataatttatc gatgatggta cgctgttcat aggcaattgt gaggtcttca gcactaattg
    10501 ttgtcatatg gttgcctcct ttctaaaaag agtcaaaggg ttgatgaaag tgcacattaa
    10561 agtgttcatc tgcccatttt aaaatagttg agaagtagct tattttctca aaatcttcac
    10621 tattatagca tagaaaaaag gcttaacaaa gccttttttg atgtgatttt taggatgtat
    10681 taaaaaagaa atattagttt agaatgatta aaaacaatag cgataatcat tctaaactaa
    10741 cgtcgacata ctctagaggt agtgtttggc gattgctaaa tcaccagcat aagctacttg
    10801 ttgtccttta acaatttgat aagcatctgc gcctttccca gcgataatca cagcatcttt
    10861 tgctccttgg caaagagaca tggctttttg aatagcttgc tcacggtcac taataatctc
    10921 taccgggcgt gcgatatggc tggcaatttc ttttgaaata tcttctggat cctcgaaatt
    10981 agggtcatca gcagtgagaa tgacggtgag gtttggatgc tgatgaatca ccctaccaaa
    11041 atcagctcgt ctgctctcgc ctttatttcc tggtgcacct aaaatcaaca ttaatttgcc
    11101 tgtctgatgt tcttccacaa cgctgagtaa tttttctaag ctgtcaccat tatgggcata
    11161 atcaacaaaa actttagcat gatttgtcat agtgaggact tccatacggc ctgggacgcg
    11221 agtcttagcg ataccttttt gaatatcagc taggctagca cccaaacgaa ggcaggcaag
    11281 tccagctgcc atagcatttt cttggttaaa atggccaatt aattgaatgt cataatggcc
    11341 agctaattgt cctttagctt caaaggagaa agcttggcta gtggtgattt gattgtcaga
    11401 caagggacca taaaacacat gctcttgatc cgctacctga tccgctagaa atgaaaaatg
    11461 atccatcccg ctattgatca caacagctct gctattctcc attaaaagac gcttatgata
    11521 gaaataatct tcaaacgttg gatgctcgat tgggccaata tgatcaggac taatatttag
    11581 gaaaacccca acatcgaacg taagcccata gacgcggcca acaagataag cctgactgga
    11641 tacttccata atcaagtggg tcatgccatt tttaacacac tcggccatca tagcaaaaag
    11701 gtctagactt tcaggagttg tcaattgtga tttgaagaaa gtttgaccgt ccaaggtagt
    11761 gttcatcgta gacaacaagg caggtttata agattctttc aaaatatggt aggcaaaata
    11821 agcagctgtt gttttgcctt ttgttccagt aaaccctaag agtttgagct gatcctgagg
    11881 attatcatag aaagccatag cgattaaact catggccttt ttaatatcag tgaccaaaac
    11941 aacgggaata tcaagcttat aatctacttc tgagacataa agaggaagtc ccttagcgat
    12001 tgcttcttta aggtattcag ccttgaatgt agctccctta gcaaaaaaaa gggtcttgcc
    12061 atctacttga cggctatcgt aactcaagcg ctcaaaactt aagccttgat aatggtagtg
    12121 gtatccgtcg gcatcaagaa cctcacgaaa attatggtct ttttttaaaa tgtctaataa
    12181 ttgttcaatg gttatcatac ccttatttta tccttaaagt tcttgtttta caagtcacaa
    12241 gcaaaagttt ataataaagc taatagaaac taggaaagaa tagatatgtc gacagaaaaa
    12301 aaacaactga cacaagaaga attgatggtt cagggagcag cctggtccac agctggtaat
    12361 ttcattagcc gacttttagg agtgttatac attattccct ggtacatttg gatggggcaa
    12421 tacgcgattc aagccaatgc tcttttcaat atggggtata atgtttatgc ttatttttta
    12481 ttgatttcaa ccacaggact taatgttgcg attgctaagc aagtcgccaa atacaattct
    12541 atggggcaga cggagcatag ctaccaattg attcggagca cgttaaaact catgcttggt
    12601 ttgggtgttg tgttttcggc aattatgtat ctaggttctc ccttatttac cagtttatct
    12661 ggtggggatg ctaagcttat tccaattatg catagtcttt ccttggctgt ccttgtcttt
    12721 ccagtcatga gtgtcattcg aggcattttt caggggcaca ataacattaa gccctatgcc
    12781 attagtcaga ttgctgagca attgattcga gtcatttgga tgcttctgac caccttcctt
    12841 atcatgaaat taggttctgg tgattacgct tcagcagtga ctcagtcaac ttttgcagcc
    12901 tttatcggga tggtggctag tatgggtgtt ctgggttact atctttggaa acagggactt
    12961 ctaacagcta tttttagtaa gccagatcat accgtttcta ttgatatcaa gggcttgttg
    13021 cttgaaacct taaaagaatc catccccttt atcgtgacag gaagcgctat tcaagccttt
    13081 cagttgattg accagtggtc ctttgtgaat accatgagtc tgtttacaga ttatagccgc
    13141 tctcaattat tggttttatt tggttatttt aacgctaatc ctgctaaaat taccatggtt
    13201 ttaatcgctg tagcagcttc tatcggaggg gttggtattg ctttgttgac agaaaattat
    13261 gtcaaaaaag acatgaaagc agctgctcgc ttaatcatta acaacattga aatgttagtg
    13321 atgtttttgt tacccgctct tactggggca attattttag caagacctct atattctgtt
    13381 ttttacggag ctagcgagga tcgtgccatt cacctctttg tggcggtcct cttccaaacc
    13441 ttgctactgg cgctttacac cctcttttca ccgatgcttc aagctctttt tgaaaatcga
    13501 aaagcgattt actactttgc ctatggtatc ttgattaagt tagttttaca gataccgctt
    13561 atttatttgc tacatgctta tggtccttta ctagcgacga cgattgcttt agtggtgccg
    13621 atttatttga tgtatcgacg cctatatcag gttactcatt ttaaccgcaa actgttgcaa
    13681 aaacgtttat tattaacctt gattgaaacc ttactaatgg gactggtcgt gtttgtggcc
    13741 aactggctat tgggctatgc ctttaaaccg acaggccgct tgaccagcct tctttacctc
    13801 ctcattattg gtggcttggg aatgacggtt tacacggcac tcaccttgct gacgcatcaa
    13861 ctggataaat taattggtag caaagctagt cgccttcgtc agaaattagg ctggcattaa
    13921 tataatgaca ccaagaacga tttcgtgagt gcttatcgaa atcgtttttt tcttaatgat
    13981 ctcgcttagt aaaggtagct gataaaaaat caggatttcc caagcaaact actgatattt
    14041 ttggtataat gataaaagtg taaaaaaata gaaggaagta tatcaacatg ggaaaatgtc
    14101 aagttatttc gcatccgctt atccaacaca aactttcaat cttacgtcgt caaaccacat
    14161 caactaaaga ttttcgtgag ttggttaatg aaattgcgat gctgatggga tatgaagtgt
    14221 cacgcgacct tcccttagaa gatgtcgaca tccaaacgcc agtctcaaaa acggtccaaa
    14281 aacagttagc tggtaaaaaa ttagctattg ttccgattct tcgtgctggt attgggatgg
    14341 ttgatggctt gttaagtttg gtaccagctg caaaagttgg ccatatcggc atgtaccgta
    14401 atgaagaaac tcttgaacct gttgaatacc tcgttaaact accagaagat attaaccaac
    14461 gacaaatttt ccttgttgat ccaatgttgg caacaggtgg ttcggctatc ttagcagtgg
    14521 attcgttgaa aaaacgtggt gcagcaaata ttaagtttgt ctgtttagtt gcagcaccag
    14581 aaggggtgaa aaaacttcaa gaagttcatc cagatattga tatcttcaca gcagcacttg
    14641 atgatcattt aaatgaacat ggctatatcg tgccaggctt gggcgatgct ggtgatcgtt
    14701 tatttgggac aaaataaaag agacaaccat caaatagtag cctttttagg ccaatcgttc
    14761 ttgattttgt ttaaaaaatc agagagattt ctcttcttta aaggaaaagc aagtttatga
    14821 tttgaccttt attgaccaaa atattataat aaagacaatc agtaaaaaag agatgaccta
    14881 gtgggttgcc gtctctcaaa atgacgctac taaaaaccct tgtagattgg agaatgtatt
    14941 atgattcctg ttgttattga acaaactagc cgtggagaac gttcttatga catttattca
    15001 cgtttattaa aagaccgtat tatcatgtta acagggcctg tcgaagacaa catggccaac
    15061 tctgtcatag cgcagctctt attccttgac gcgcaagaca acacgaaaga tatctaccta
    15121 tatgtcaaca caccaggtgg ctctgtctca gcaggcttgg ctattgtcga taccatgaac
    15181 tttatcaagg ccgatgtgca aactattgtc atgggtatgg cagcttctat ggggacagtc
    15241 attgcttcat caggaacaaa aggcaaacgc tttatgttac caaatgcaga gtatatgatc
    15301 caccagccaa tgggtggcac aggcggtggc acgcaacaaa cggatatggc tatcgcagca
    15361 gaacatctct taaaaacacg tcaccgttta gaaaaaatct tagcgcaaaa tgctggaaaa
    15421 acgattaaac aaatccataa agatgctgag cgtgattatt ggatgagtgc tgaagaaacc
    15481 ttggcttacg gtttcattga tgaaatcatg gaaaacaacg aactaaaata aagaggatct
    15541 ctgcaaaagt attctagaaa cttctaggat gctttttctc ttagaaagga aaataatgat
    15601 acagcctact gcattaaaac agcttcaaga gttaaaacgt cagttcagat ccctttgcaa
    15661 ccaattatga gatagtttga ggatgatcct tagcagctgt gtgcaagaag ttatgacgat
    15721 ttcataaagg atgacctagc cagtctttct tcgtctcttt tacttaaagt gtggtataat
    15781 cagattatgc tagccaaaac taaaaaatgc attgccatta aaggtgttgt agatcggctt
    15841 atcctagatc tcataacaat gctgtagtac cgcccttacc ttgctactaa aggcgctgtt
    15901 gaagcacaaa ttgacttgca aaggatcttg actgataggt atatgtccgg tggaatcgct
    15961 aaagcagcaa aaagaccaag acccttcttt ggtctctacc aaaatataat agaaaaggat
    16021 ttagaatgtt tcaaaaacaa gaacgtattg gactcgtcgt ttacctgtat tataaccgag
    16081 atgccagaaa gttatcaaaa tttggtgatt tatattacca ttccaagcgc tctcgttacc
    16141 tcattattta tattaataaa aatgatttag acacaaaatt agaagaaatg agacgtttga
    16201 aatgtgttaa agacattaga ccatcagctt ttgatgatat tgaccgccaa tttgtaggta
    16261 accttcatcg cgacgaaaca aacaaccatc aaaagggtta acacccccct agttactgat
    16321 tagcgtttca gatttagtta gcttattaaa gtaagaaggt tttgacagat gctcacacta
    16381 cttcaagaca atgagacaac tagagcatta gtgaataact gagttgactg tcacttcttt
    16441 aataagaacg gataggattg gtcctaaaat agagcactgc aaagatatgc cagtgctttt
    16501 tagctcattt tttgataaaa tagaactact aaaaagtgaa ggaaataaga tgatcacagg
    16561 aaaactcata accgttgaag gtccagatgg cgcagggaaa acgactgttt tagagcaact
    16621 cattccactg ttaaaacaaa aagtggctca ggagatcttg acaacaagag agcctggagg
    16681 tgttgccatt tcagaataca ttagagaact tattttagac atcaaccata ctactatgga
    16741 ccctaaaaca gaactcttac tttatattgc ggctcgaagg cagcatttgg tggaaaaggt
    16801 actaccagct cttgaagcag gccagttggt ttttattgat cgtttcatcg atagttcagt
    16861 agcttatcaa ggtgcgggac gcggtttgac caaagcagac attcagtggc tcaacgagtt
    16921 cgcaacagat ggtttagagc ctgacttgac cctttatttt gatgttcctt ctgaaattgg
    16981 tttagcacgg attaacgcga accaacaaag agaagtcaat cgtttagatt tggaaacaat
    17041 agagatacat caacgagtac gaaaaggata cctagcctta gctaaggagc atccaaaacg
    17101 tattgtcaca attgatgcga ctaaaccatt gaaagaagtt gtctctgtcg ctttggaaca
    17161 tgtcttagcc ttgcttttag cctaggataa gggagggtag gaatggattt agcgcaaaaa
    17221 gctcctaacg tttatcaagc ttttcagacg attttaaaga aagaccgtct gaatcatgct
    17281 tatctttttt cgggtgattt tgctaatgaa gaaatggctc tttttttagc taaggtcatc
    17341 ttttgtgaac agaaaaagga tcagacgccc tgcgggcatt gtcgctcttg tcaattgatt
    17401 gaacaaggag attttgccga tgtgacggta ttggaaccaa cagggcaagt gattaaaacg
    17461 gatgtggtca aagaaatgat ggctaacttt tctcagacag gatatgaaaa caaacgacaa
    17521 gtttttatta tcaaagattg tgacaaaatg cataccaatg ccgctaatag cttgctaaag
    17581 tatattgagg agcctcaggg agaagcttac atgtttttgc tgaccaatga tgataacaaa
    17641 gtgcttccga ccattaaaag tcggacacag gtttttcagt ttcctaaaaa cgaagcctat
    17701 ctttaccaat tggcacaaga aaagggatta ttaaaccatc aggctaagct agtagccaaa
    17761 cttgccacaa acaccagtca tctagaacgt ctgttgcaaa cgagcaagct tttagaactg
    17821 ataactcaag cagagcgttt tgtatctatt tggctgaaag atcagttgca ggcatattta
    17881 gcgttgaacc gtctggtaca gttagcaact gaaaaagaag aacaagattt agttttgacc
    17941 cttttgacct tgctcttggc aagagagcgt gcgcaaacgc ctttgacaca attggaggct
    18001 gtttatcagg ctaggctcat gtggcagagc aatgttaatt ttcaaaacac attagaatat
    18061 atggtgatgt cagaataagt gcaattggaa ttcgttatgg ggaaacgaag cttgtcaaaa
    18121 gacttgccct tagcgaccaa agtagcggcg cataggggca aaacaagctt ttgaagaaaa
    18181 tactgccttt gcaacagatt tgatgaaaga agtatcacga ttaataacac aaaatcaact
    18241 tgaaatgacg tttattaata gtgtctttcc cttagatcag catgttgtcc taattccctt
    18301 ttcagcagaa gagcacatgg atttttacaa ctgttaaaag atttagcggc cgattttagg
    18361 tcaagaagtg agctgcccca gattaacagc caagaagaaa ccaagattta tgggcgctta
    18421 ggaccgtgtg gcagaccact gtgctgcttt cgtttttggg aaaatttctg acagtatcta
    18481 ttaaaatact aaaaaatcaa aggttatcat tgactttggg taaagcaaca agcccttatg
    18541 gccgtttgct ttgctgtctc tatgatgaag gtggctttta ccaataaagc aagaaaaaat
    18601 tccctgaata tggtaccata gtagagacaa gtgatggccc tgcaacagta gcagccttga
    18661 tatatttaca gatcccatta aggtgcggtt aaaagaccag ttgaccttag tcacttatgc
    18721 tttagaggag gttaaagtag gtgaataaaa aagaattatt cgatgctttt gatggatttt
    18781 cccaaaattt aatggttact ttagctgaaa ttgaagccat gaaaaaacaa gtccaatcac
    18841 tagtagaaga aaacacgatt cttcgcctag aaaatacaaa actacgggaa cgtttgagtc
    18901 acctagaaca tgaaacggta gctaaaaatc cttccaaaca aaggaaggat cacttagagg
    18961 gaatttatga tgaagggttt catatctgca attttttcta tggacaacgt cgtgaaaatg
    19021 atgaagaatg tatgttttgc agagaattat tagatagaaa gtaagggcat gcaggttcaa
    19081 aaaagtttta aagacaaaaa aacaagcgga accttgtatt tggttccaac cccgattggc
    19141 aatctgcagg acatgacctt tagagcagtg gcaacgttaa aagaggttga ttttatttgt
    19201 gcagaagaca caagaaacac aggtcttttg ctaaaacact ttgacattgc taccaagcaa
    19261 atcagctttc atgagcacaa cgcctatgag aagattcctg atttaattga tttattaata
    19321 tcaggacggt cattagctca ggtgtctgat gcaggaatgc catcgatttc tgatcctggg
    19381 catgatttgg tcaaggctgc tattgatagt gatatcgcag tcgttgcttt accaggtgcc
    19441 tcagctggga ttacagcttt gattgctagt ggactggctc ctcaacccca tgttttttat
    19501 gggtttttac ccagaaaagc agggcaacaa aaggctttct ttgaggacaa acaccattac
    19561 ccagagacac aaatgtttta tgaatcaccc tatcgcatca aagataccct gaccaatatg
    19621 ttggcttgtt atggtgatcg tcaagtggtg cttgtcagag aattaaccaa gctttttgag
    19681 gagtaccaac ggggggcgat ttcagaaatc ttgtcctacc ttgaggaaac acccctaaaa
    19741 ggggagtgtc tgctgattgt ggctggagcc caagtagaca gtgaggttga gctgacagct
    19801 gatgttgact tagtgtctct agtgcaaaaa gaaatccaag caggagccaa gccaaatcag
    19861 gctattaaaa caattgctaa agcttaccag gtaaatcgcc aagagcttta ccaacaattt
    19921 catgacttat aagtcatcgt agattaggag aaaaacaaat gacagaagat caagcaacta
    19981 tccttatttt aggagtatac ggaatcatat tggctgtatt cttattatca gctttattat
    20041 gccttatcgc taactggcga ctctttaaaa gagcaggtta caaaggatgg tatagtctca
    20101 ttcctttcta tgcaggtttt gtcaaacaca aaatcacttt tggagaagat aataaatggt
    20161 tttactttat tggttgggtc attgctattt attattacta tgtgcacttt tgctatgtcc
    20221 gtgcttttgg tggatcaaag ggtctagcag tatttggctt gtttttccca ggcattgctt
    20281 ctttgatagt ggcttttgga aaaacttata ccacacaaga atgccaccct gtgccacatc
    20341 ttttaaacta agagagaaaa gactatcaat tggatttatt gatggtcttt taggtgttta
    20401 aatgatttta gtcccgtgaa caatggccgt tccagtgagt tgactcaagc tttggcaatt
    20461 ctcagcagtg acgcctcctc caatcatgat ttcaatgcgt ttattggcat aggtcacaag
    20521 acttttaagt tgttccacat tgtctgtaat aggagtagct tcaggggagc catgagttag
    20581 cacacgcaca aagccataat caatcagttg atcaagcgct tggtgctggt gatcagtcgg
    20641 aatatggtca aaagccatgt ggaaaaccag tggcaagcct tgtgtagcag gcagtaattg
    20701 ctcgatagcg tcggtatcca gttgattttc tgtggtcagc aagcctaaga ctagcgcatc
    20761 tgagccagct tcaacagctt ttaagatatc ttcttccata gcttttagtt cgaggtcgtt
    20821 atagacaaaa tcgccaccgc gaggtcgaat catggtcgct acgctaatct ttttatcgtg
    20881 taagagctga caggcttctt tgatgacgcc ataagatggc gtggtccctc cgacagctag
    20941 gttatcacag agttccacac gactgacttg accagcatca agggttggca gtagtgtgag
    21001 gttctcagca caaaattctt taatcatttt tagttctcct ttatcactaa tattatagca
    21061 cgaatgaagc tagtcatcag gaaacatttt tgtcaaatca tggacattat gctatactca
    21121 ctagtattaa aagtaggcca aaacagcagc aaatactttg aaaaaagctt gctatgttct
    21181 acggcaatat cagtctttac caacgaactc tcaagaagaa aagaaagaca aagcttagca
    21241 ttttaaaaaa gcctcccctt tttttgtgaa atgatgacca aaaacaggcg cctcacgcct
    21301 aaaacagtta ccctaaagga gaaaaaatga ttacgattta ccaataccca aaatgtagca
    21361 cctgccaaag agccatggct gaattaaagc aactggttag tgactttgaa gccattgaca
    21421 tcaaagctaa tccacccaaa gctcaggacc tcaaacattg gatggaaaca tctggttata
    21481 ccatcaaaaa tttctttaat accagtggta acagctaccg cgaattggga cttaaagata
    21541 aaatagacca gcttagcctt gataaagctg ctgaactctt agccacagat ggtatgctga
    21601 ttaaacggcc tattctgatt aaggacggta aagtcctaca agttggctat cgcaagccat
    21661 atcaagagtt ggatttagga taatgcgaca aagtctcaaa aaaggacaag gcaacattat
    21721 aacgccttgt ccttttttgg gatctgtttt tagacattag agatcaatat ctagtaaaat
    21781 tggtgtatgg tcttggcgtt ccccagaaga aatcatttca gaacgcttaa ccttatccac
    21841 gattcggttt gaagctaggc aatagtcaat tctccaaccg gtattattga ttttgctggt
    21901 tttgctacgt tgtgcccacc aggtataaac atgaggaata tcgccgtgca agtgacgaaa
    21961 agtatctgta aagcctcttg ctaataggtt agtaaagcct tgacgttctt cgtcggtaaa
    22021 accaggggaa cgtcgattgc tgcttggatt tgccaaatca atctctttgt gggcaacgtt
    22081 gtaatctccc gtagccaaaa caggcttttg agtatcaagt tcagtcaagt aatcagcgta
    22141 tttgtggtcc cagatttgac ggtcatctaa ccgtcttaga ccatcaccag cattaggcgt
    22201 gtaaacctgt gtgacgaaaa agtcttcaaa ttctagggta atgatacggc cctcagcatc
    22261 cattgttgtt ggggcaccaa tttctggaaa agtaatgaca gggtttaacg tgtttttgta
    22321 caggaacatg gtgccagcat agcctttcct agcaggctca actgaagaac gccacacatg
    22381 caaatagttt ggaaagtaac taagtagggt ctcaatatgt tttttggtag gccccttagc
    22441 agataatttg gtttcttgaa tggcaataat atccgcatct tgagcgacta aagtgtctaa
    22501 aacagcgcgt gataaaagcg cacgagggga ctctcctgtg agagcagcat ttaaggaatc
    22561 aatgttccat gaaatcagtt tcatttggtt tctctttcta acagatagtc ttgttgttat
    22621 tgtaacaaaa aacgaaacga taagcgactt tgggcaccta gcccccataa aacagtctag
    22681 ccaaatgaag catagcagcg taagaacaag acaaggaggt gatcagtgag catatcgttt
    22741 gacagttaag agtaggatta ctataataga aacacaaatg taagcgctaa caaaataaaa
    22801 aatataggag aatgtcaatg gcacaaaaaa cagtcatcac cgaagaaaca accgattttg
    22861 ttatggattt caaaacaagt agcgctgagg gaaatgttga ttttattaac gtttttgacc
    22921 ttgagaaaat ggcacaacag gtcatcccta aaggagcctt tggttatatt gctagtggtg
    22981 ctggtgatac ctttaccctc catgaaaata ttcgctcatt taatcataaa ttaattgtcc
    23041 ctcactccct caaaggggtt gaaaatccgt caacagagat tacgtttgat ggtgattact
    23101 taacctctcc tcttatttta gcacctgtag cggctcataa actggccaat gaacaaggag
    23161 aagtagcgtc tgctaaagga ttgaaggaat ttggaagtat ctacaccact agttcctact
    23221 caacaactga cctgccagaa atcagtgctg ccttaggtgg gacaccccat tggtttcaat
    23281 tttattacag taaagacgat ggcattaatc gtaatattat ggatcgtgtc aaagcacaag
    23341 gttgcaaagc tatcgttttg acagcagatg caacagttgg aggaaaccgc gaagttgata
    23401 ggcgaaacgg ttttgttttt ccggtcggta tgccaattgt tcaagaatat ttacctgatg
    23461 gtgcaggtaa aacaatggat tatgtctata aatcagctaa acaagcgctt acctcaaaag
    23521 atattgaata tatcgcaact tactcaggcc tgcctgttta tgtcaaagga ccacagtgtg
    23581 ctgaggatac cttgcgggca ttggacgcag gagcatcagg tatctgggta accaatcatg
    23641 gtggccgtca attagatggt ggtccagctg cttttgattc cttacaagaa gtcgctgaag
    23701 cagttaatca aaaagttcct attgtctttg actcaggtat aagacgaggt caacatattt
    23761 tcaaagctct tgcttcaggt gctgaccttg ttgccttggg gcgtccagcg atttacggct
    23821 tggctatggg aggaagcatt ggtactcgtc aggtctttga aaagttaaat gacgagttaa
    23881 aaatggtcat gcaactggct gggacacaaa ctatccaaga tgttaaagcc tttaaccttc
    23941 gccacaatcc gtatgattca tcgattccat ttgatcaaaa tgcgttacgc ttagattaga
    24001 acagacaaaa acctccgcta tcatgtcaca aatagtagag gttttacact gcaaaaagag
    24061 gtatgaaaat ccaataacat agttacaagg ctttcgctta ccaaccccta ctaatagatt
    24121 aaaatgaaag acttcttaca ttaatagaaa tttctgttgc ttttctcaga aaaatgtggt
    24181 taaataatca aatattatta acgaaaaatg agttaatgat taacattaaa ctggttttag
    24241 ttaaaacatt taggagggta tcaggtggag aaaaagcaac gtttttccct tagaaaatac
    24301 aaatcaggaa cgttttcggt cttagtagga agcgttttct tgatgatgac aacaacagta
    24361 gcagcagatg agctaaccac aacgagtgaa ccaacaatca cgaatcacgc tcaacaacaa
    24421 gcgcaacctc tcaccaatac agagttgagc tcagctgaat cacaatcccc agacacatca
    24481 caagtaactc cagagacaaa tcgtgaaaaa gagcaatcac aagatctagt ctctaagcca
    24541 accacaactg agctagctga cacagatgca gcaccaatgg ctaatacagg tcctgatgcg
    24601 actcaaaaaa gcgcttcttt accgccagtc aatacagatg ttcacgattg ggtaaaaacc
    24661 aaaggagctt gggacaaggg atacaaagga caaggcaagg ttgtcgcagt tattgacaca
    24721 gggatcgatc cggcccatca aagcatgcgc atcagtgatg tatcaactgc taaagtaaaa
    24781 tcaaaagaag acatgctagc acgccaaaaa gccgccggta ttaattatgg gagttggata
    24841 aatgataaag ttgtttttgc acataattat gtggaaaata gcgataatat caaagaaaat
    24901 caattcgggg attttgatga ggactgggaa aactttgagt ttgatgcaga tgcagagcca
    24961 aaagccatca aaaaacacaa gatctatcgt ccccaatcaa cccaggcacc gaaagaaact
    25021 gttatcaaaa cagaagaaac agatggttca catgatattg actggacaca aacagacgat
    25081 gagaccaaat acgagtcaca cggtatgcat gtgacaggta ttgtagccgg taatagcaaa
    25141 gaagccgctg ctactggaga acgcttttta ggaattgcac cagaggccca agtcatgttc
    25201 atgcgtgttt ttgccaacga cgtcatggga tcagctgaat cactctttat caaagctatc
    25261 gaagatgccg tggccttagg agcagatgtg atcaacctga gtcttggaac cgctaatggg
    25321 gcacagctta gtggcagcaa gcctctaatg gaagcaattg aaaaagctaa aaaagccggt
    25381 gtatcagttg ttgtagcagc aggaaatgag cgcgtctatg gatctgacca tgatgatcca
    25441 ttggcaacaa atccagacta tggtttggtc ggttctccct caacaggtcg aacaccaaca
    25501 tcagtggcag ctataaacag taagtgggtg attcaacgtc taatgacggt caaagaattg
    25561 gaaaaccgtg ccgatttaaa ccatggtaaa gccatctatt cagagtctgt cgactttaaa
    25621 gacataaaag atagcctagg ttatgataaa tcgcatcaat ttgcttatgt caaagagtca
    25681 actgatgcgg gttataacgc acaagacgtt aaaggtaaaa ttgctttaat tgaacgtgat
    25741 cccaataaaa cctatgacga aatgattgct ttggctaaga aacatggagc cctgggagta
    25801 cttattttta ataacaagcc tggtcaatca aaccgctcaa tgcgtctaac agctaatggg
    25861 atggggatac catctgcttt catatcgcac gaatttggta aggccatgtc ccaattaaat
    25921 ggcaatggta caggaagttt agagtttgac agtgtggtct caaaagcacc gagtcaaaaa
    25981 ggcaatgaaa tgaatcattt ttcaaattgg ggcctaactt ctgatggcta tttaaaacct
    26041 gacattactg caccaggtgg cgatatctac tctacctata acgataacca ctatggtagc
    26101 caaacaggaa caagtatggc ctctcctcag attgctggcg ccagcctttt ggtcaaacaa
    26161 tacctagaaa agactcagcc aaacttgcca aaagaaaaaa ttgctgatat cgttaagaac
    26221 ctattgatga gcaatgctca aattcatgtt aatccagaga caaaaacgac cacctcaccg
    26281 cgtcagcaag gggcaggatt acttaatatt gacggagctg tcactagcgg actttatgtg
    26341 acaggaaaag acaactatgg cagtatatca ttaggcaaca tcacagatac gatgacgttt
    26401 gatgtgactg ttcacaacct aagcaataaa gacaaaacat tacgttatga cacagaattg
    26461 ctaacagatc atgtagaccc acaaaagggc cgcttcactt tgacttctcg ctccttaaaa
    26521 acgtaccaag gaggagaagt tacagtccca gctaatggaa aagtgactgt aagggttacc
    26581 atggatgtct cacagttcac aaaagagcta acaaaacaga tgccaaatgg ttactatcta
    26641 gaaggttttg tccgctttag agatagtcaa gatgaccaac taaatagagt aaacattcct
    26701 tttgttggtt ttaaaggaca atttgaaaac ttagcagttg cagaagagtc catttacagg
    26761 ttaaaatctc aaggcaaaac cggtttttac tttgatgaat caggtccaaa agacgatatc
    26821 tatgtcggta aacactttac aggactcgtc actcttggtt cagagaccaa tgtgtcaacc
    26881 aaaacgattt ctgacaatgg tctacacaca cttggtacct ttaaaaatgc agatggcaaa
    26941 tttatcttag aaaaaaatgc ccaaggaaac cctgtcttag ccatttctcc aaatggtgat
    27001 aacaaccaag attttgcagc cttcaaaggt gttttcttga gaaaatatca aggcttaaaa
    27061 gcaagtgtct accatgctag tgacaaggaa cacaaaaatc cactatgggt cagcccagaa
    27121 agctttaaag gagataaaaa ctttaatagt gacattagat ttgcaaaatc gacgaccctg
    27181 ttaggcacag cgttttctgg aaaatcgtta acaggagctg aattaccaga tgggtattat
    27241 cattatgtgg tgtcttatta cccagatgtg gtcggtgcca aacgtcaaga aatgacattt
    27301 gacatgattt tagaccgaca aaaaccggta ctatcacaag caacatttga tcctgaaaca
    27361 aaccgattca aaccagaacc cctaaaagac cgtggattag ctggtgttcg taaagacagt
    27421 gtcttttatc tagaaagaaa agacaacaag ccttatacag ttacgataaa cgatagctac
    27481 aaatatgtct cagtagcaga caataaaaca tttgtggagc gacaagctga tggcagcttt
    27541 atcttgccgc ttgataaagc aaaattaggg gatttctatt acatggtcga ggattttgca
    27601 gggaacgtgg ccatcgctaa gttaggagat cacctaccac aaacattagg taaaacacca
    27661 attaaactta agcttacaga cggtaattat cagaccaaag aaacgcttaa agataatctt
    27721 gaaatgacac agtctgacac aggcctagtc acaaatcaag cccagctagc agtggtgcac
    27781 cgcaatcagc cgcaaagcca gctaacaaag atgaatcagg atttctttat ctcaccaaac
    27841 gaagatggga ataaagactt cgtggccttt aaaggcttga aaaataacgt gtataatgac
    27901 ttaacggtta acgtatacgc taaagatgac caccaaaaac aaacccctat ctggtctagt
    27961 caagcaggcg ctagtgcatc cgctattgaa agtacagctt ggtatggcat aacagcccga
    28021 ggaagcaagg tgatgccagg tgattatcag tatgttgtga cctatcgtga cgaacatggt
    28081 aaagaacatc aaaagcagta caccatatct gtgaatgaca aaaaaccaat gatcactcag
    28141 ggacgttttg ataccattaa tggcgttgac cactttactc ctgacaagac aaaagccctt
    28201 ggctcatcag gcattgtccg cgaagaagtc ttttacttgg ccaagaaaaa tggccgtaaa
    28261 tttgatgtga cagaaggtaa agatggtatc acagttagtg acaataaggt gtatatccct
    28321 aaaaatccag atggttctta caccatttca aaaagagatg gtgtcacact gtcagattat
    28381 tactaccttg tcgaagatag agctggtaat gtgtcttttg ctaccttgcg tgacctaaaa
    28441 gcggtcggaa aagacaaagc agtagtcaac tttggattag acttaccggt ccctgaagac
    28501 aaacaaatag tgaactttac ctaccttgtg cgggatgcag atggtaaacc gattgaaaac
    28561 ctagagtatt ataataactc aggtaacagt cttatcttgc catatggcaa atacacggtc
    28621 gaattgttga cctatgacac caatgcagcc aaactagagt cagataaaat cgtttccttt
    28681 accttatcag ctgataataa cttccaacaa gttaccttta agatgacgat gttagcaact
    28741 tctcaaataa ctgcccactt tgatcatctt ttgccagaag gcagtcgcgt tagccttaaa
    28801 acagctcaag gtcagctgat cccgcttgga cagtccttgt atgtgcctaa agcttatggc
    28861 aaaaccgttc aagaaggtac ttacgaagtt gttgtcagcc tgcctaaagg ctaccgtatc
    28921 gaaggcaaca caaaggtgaa tgccctacca aatgaagtgc acgaactatc attacgcctt
    28981 gtcaaagtag gagatgcctc agattcaact ggtgatcata aggttatgtc aaaaaataat
    29041 tcacaggctt tgacagcctc tgccacacca accaagacaa cgacctcagc aacagcaaaa
    29101 gccctaccat cagcgggtga aaaaatgggt ctcaagttgc gcatagtagg tcttgtgtta
    29161 ctcggactta cttgcgtctt tagccgaaaa aaatcaacca aagattgata acaaagcgca
    29221 aagagacaac aaatcatccc ttttaacagt ctgacctttc tttgagattg agctggcaga
    29281 tgatcatttc ttaacaaaag ttaaagagtt gagcaaagta aggaacagac ataaagacca
    29341 taaaagcaag ccagtttggc ttgcttttat ggtgttattg tcgtgtgcta cgatgactga
    29401 aaaagagcaa cgaacaatga cctcgaatca ccaaaaggac ttgatttgat agcttcccta
    29461 tgctatacta agcctattct ttcaagaagg actccttttg tggaaaagac tgaaaaaaag
    29521 gacactaatc tatccaaaaa ctccagatga gtagctgcat gagtagctgc aaccggatgc
    29581 ctctgctgtg atcaaggtgg taaagcagac tggcttgtca ctacctgagc aggtgtcaaa
    29641 aaaagaagct ttggctttgt cattcaacat actgagcttt agtctttcag aacggagaca
    29701 cccccagttt tgtactcaaa aagcataaac agctctgctg atgcgacatc cgatttctac
    29761 gtccgcagtg agccaaacca gctgctgttt gctatggtaa aagtggcgcc aatcgggatt
    29821 tttgagatcc taggctcaaa aataaggcat agaattccaa aggaagtcgc tgccgtgagc
    29881 acaacctaag aaaaactatt ccaaaagact ttttctgcaa tttgaaatac tgaatttcag
    29941 tattttttct tttgtaacta agagcaatga atgaggtaaa aaaaggcaac aatgtcaaaa
    30001 aagaaacgaa aacactatca ggtttatgag ggattacgct gtgctatgac gctttgtttt
    30061 atcagtggct atgtcaatgc ctttacctat atgacacaag gcaaacgttt tgcaggtgtt
    30121 cagacaggca accttttgtc atttgccatt cgcttgtcag aacagcaact gaaggaggca
    30181 ctgcaatttt tacttcccat gatagttttt atgcttgggc agtcctttac ttatttcatg
    30241 caccgctggg ccactaaaaa agggctccac tggtacttat tatcaagctt tatcttaaca
    30301 gggattgctt ttggaacggc cctattcacg ccttttcttc cttccaatgt tacggtagca
    30361 gctttagcct tttttgcctc tattcaagtt gatacgttta aaaccttacg aggagctagc
    30421 tatgctaatg tgatgatgac aggaaatatc aaaaatgccg cctatttact aaccaaaggc
    30481 ttgtacgaaa aaaatcacga actaacacat atcggacgca acaccttaat agtcattttg
    30541 gcttttgctg ttggggtggt ttgttcgacg ttgctttgca tagcttatgg cgagtatgct
    30601 ttgataccta ttttaatgcc tttactctat gtcaattacc tgttggctca ggaattttat
    30661 cacattcaaa ccaaaatcaa accgattaaa agaacttaac ggacttgtgg agaaagaaag
    30721 ttcatttagg gtaagttttt actgaccctc cgttgatttg ctggttttgc tgccccttaa
    30781 aatatgatat aatagaccat tgatagctgt tacttaacag aaggctgata aaaaaggttc
    30841 cattagcctt gagggttgaa tttaaacagg caaaaacgaa atcctacact atgcatctca
    30901 atcatacttt catttgagta tggttctgaa aggaaactat gatgaaaaaa ccattttaca
    30961 ttacgacacc catttattac ccatctggga aattacacat tggttctgcc tatacaacta
    31021 ttgcttgtga tgtcttggca cgctacaagc gcctgatggg ccatgaggtc ttttacctca
    31081 caggactaga tgaacacgga caaaagattc aaaccaaggc aaaagaagct ggtattacac
    31141 ctcaaaccta cgttgataac atggcaaagg atgtcaaagc cctttggcaa ttgctagata
    31201 tttcctatga cacatttatc cggacaactg acgattacca cgaagaagtc gttgctgctg
    31261 tttttgaaaa attgctggcg caagacgaca tttaccttgg cgaatactct ggttggtatt
    31321 ctgtctctga tgaggaattt ttcactgaaa gccaattaaa agaagtcttt agagatgaag
    31381 acggtcaggt tattggaggg attgccccat caggtcatga ggtcgagtgg gtctctgaag
    31441 aatcctattt cctacgcctt agcaaatacg ctgattgtct tgtggccttc tttaaagagc
    31501 gacctgactt tatccaacca gatggtcgta tgaatgaaat ggtgaaaaac tttatcgagc
    31561 caggtctaga agatttggct gttagccgta ctacctttac atggggtgta cctgttccat
    31621 cagacccaaa acacgtggtt tatgtgtgga ttgatgccct cttaaattac gcgacagctc
    31681 ttggttatgg tcaagctaat cacgctaact ttgataaatt ctggaatgga acggttttcc
    31741 acatggttgg aaaagatatc ttacgttttc atagcatcta ctggccgatc ttactcatga
    31801 tgcttgattt accaatgcca gaccgcttaa ttgctcatgg ttggtttgtt atgaaagacg
    31861 gtaaaatgtc taaatcaaaa ggcaatgtgg tctatccaga aatgttagtg gagcgctttg
    31921 gacttgatcc tcttcgttat tacctcatgc gttctttgcc agtgggttca gacgggacct
    31981 ttactcctga agattatgtg ggtcgtatca actatgagtt agccaacgac cttggtaatt
    32041 tattgaatcg taccgttgcg atgatcaaca agtattttga tggcacggtg ccagcttacg
    32101 ttgacaacgg aactgccttt gatgctgact taagtcaact gattgatgct cagctagcag
    32161 actatcataa gtacatggaa gcggtggatt acccacgcgc cttggaggct gtttggacca
    32221 tcatcgctcg taccaacaaa tacatcgatg aaaccgctcc ttgggtgctc gctaaagaag
    32281 atggtgacaa agctcaacta gccagtgtca tggcacactt ggcagctagc ttgcgcgtgg
    32341 tcgctcatgt gatccaaccc tttatgatgg aaacatcagc tgctatcatg gcccagcttg
    32401 gcttagcgcc tgtttctgac ttgtcaacac ttgctctagc agcttttcct gccaatacaa
    32461 aggtcgttgc caaaggcaca ccgattttcc cacgtcttga catggaagct gagattgact
    32521 acatcaaagc tcaaatgggt gacagctcag ctatttccca agaaaaagaa tgggtccctg
    32581 aagaggttgc gctcaaatct gaaaaagacg tcatcacatt tgaaaccttt gatgctgtgg
    32641 aaatccgggt ggcagaagtc aaggaagtgt caaaagtgga aggttcagaa aaattgctgc
    32701 gctttagagt agatgcagga gacgggcaag accgtcaaat cttgtcaggt attgccaaat
    32761 tttaccccaa cgaacaagaa ttagtgggca aaaaactaca aattgttgct aaccttaaac
    32821 ctcgtaaaat gatgaaaaaa tacatcagcc aaggcatgat tttatcagca gagcatggag
    32881 atcagctaac ggtcttaacc gttgattcct ctgttccaaa tggaagtatc attggttgat
    32941 gctgacctat tattcaaaaa acctaagcaa gaatatgtgc ttaggttttt ttgattgcct
    33001 ttaagaaaga cgaataacga gatgggtgat atggcatcaa aagcataact atcctaggac
    33061 ctgtcctagt aagattgctg tgcttttgac aagtgaacag agaggtctta aactatatct
    33121 tgtcataaaa aagattgcaa aacactatat cttgtgttat tatggttttt ataaaagtta
    33181 agcatgctta actttagcca aacaaaagag gacactagag cgtgtcccac aagacagttg
    33241 agcccttcgc ttttttagta gagtaggagt gctcaagtca aaaagagaca tcccatcaaa
    33301 ccattgctgt ttagcaggtc tttttgtgtt gctcaaaaag acaattcctg tctgtttggc
    33361 gtctagtgat gacttaggct tagcgctact agacttttgt ttgaaaaata cctcaagcct
    33421 tgaaggagac aaatgaccca acattattat gaacgttcgc aatccccaat tgaatacgcc
    33481 ctatctgaaa cacaaaaaca gttgcgctcc atcaattgga attaccttaa tgatgacaaa
    33541 gatttggaag tgtggaaccg tgtcactcaa aatttttggt tgcctgaaaa ggttcctgtt
    33601 tctaatgatt taaattcttg gcgctccttg ggtgaggatt ggcaacagct catcacaagg
    33661 acctacactg ggttgacttt actagataca gtacaagcaa cggttggtga tgtggcacaa
    33721 attcagcatt cacaaaccga ccacgaacaa gtcatctata cgaactttgc ctttatggtg
    33781 gggatccatg cccgctctta cgggacgatt ttttcgaccc tttgcagtag cgagcaaatc
    33841 gaagaagcac atgaatgggt ggtctcaaca cagagcttgc aagaccgtgc ccgtgtcttg
    33901 attccttact atacgggtga tgaccctttg aaatcaaaag tagcagcagc tatgatgcca
    33961 ggatttttgc tgtatggcgg cttttacctt cctttttacc tatcagcacg tggtaaaatg
    34021 ccaaacacat ctgatatcat ccgcctgatt ttacgcgaca aagttattca caactattac
    34081 agcggttaca aataccaaca aaaagtagca cgtcttagcc cagaaaaaca ggctgaaatg
    34141 aaggcctttg tctttgactt gctctatgaa ctcatcgacc ttgaaaaagc ttacttgaga
    34201 gaactgtatg caggctttga tttggcagaa gatgctatcc gctttagcct ttataacgct
    34261 ggaaaattcc ttcaaaattt aggctatgat tcgcccttca cagaagaaga gacccgtatc
    34321 tcacctgaag tatttgctca gctatcagcg cgtgcagacg aaaaccatga ctttttctca
    34381 ggcaatggct catcttatgt gatggggatc accgaagaaa ccacagatga cgattgggag
    34441 ttttaagaaa tggcagagct gattattgtg tatttttcat ctaagtccaa taacacccat
    34501 cgcttcgtcc aaaaattagg cttgcctgct cagcgcatac cagtagataa taggccactt
    34561 gaagtttcaa cccattacct tttgattgtg cctacctatg cagctggtgg ctcagatgcc
    34621 aaaggagctg tccctaaaca agtgattcgt tttttgaata atcctaacaa tcgtaaacat
    34681 tgtaagggag tgatctcttc aggaaatacc aattttggcg atacctttgc cttagcaggc
    34741 cctatcattt ctcaaaaatt gcaggtcccc ttattacacc aatttgaact attaggcact
    34801 gctacagatg tcaaaaaggt acaggcgatt tttgcccgct taaagcatca cacacacgac
    34861 aagcaaaaac aaaccaacaa cctgattaca gaaaggacac acccatgtca caaaccaatg
    34921 cgtcatacct ctcattaaat gcactgactc gttttaaaaa accagacggc agctaccatt
    34981 ttgatagcga taaagaagct gttaggcgct acctggaaga acatgtctct cccaatcaaa
    35041 tggcttttaa ctcactagaa gataagctag cttacctcat caatgaaggg tattatgagc
    35101 aggccatttt tgatgcctac cctaacgacc ttatcaagga ggcctttcat tatgcctatc
    35161 aacaaggcta tcgcttttta aatctcatgg gcgctatgaa attttaccag tcctatgcct
    35221 taaaaacctt ggatggtaag cagtatctag aaacatttga agaccgtgct gtgatgaatg
    35281 cactcttttt agctgatgga gatcaaacct ttgtctttga cgtgattgat gccatcttgc
    35341 accgacgctt tcaaccagct accccaacgt ttttaaatgc cggaaaaaaa cgccgtggcg
    35401 aatacatttc gtgctatctt cttcgtgtcg aagacaatat ggaatctatc tctcgcgcca
    35461 ttagcaccag cttgcaactt tcaaaaagag gaggcggagt cgccttgtgt ctcaccaacc
    35521 tcagagaaat cggagcaccg ataaaaggga ttgaaaatca ggcaacaggc attgttcctg
    35581 tcatgaaact actagaggac tctttttcct atgccaatca actaggtcag cgccaaggag
    35641 caggtgcggt ttaccttcat gcccatcacc ccgaggtcct gaccttttta gacaccaaac
    35701 gtgaaaatgc cgatgagaaa attcgcatta agtccctagc attgggactt gtgatcccag
    35761 atattacctt ccagttagcc aaagaaaaca aagacatggc actcttttca ccctatgata
    35821 tcaagcgtgc ctatggcaag gacatgtcag acatctccat cacagaggaa tacgataaac
    35881 tgctagtcaa tccagccatc aaaaagacct acatcagtgc tagaaagttc tttcagttca
    35941 ttgcagagct acattttgag tcaggctacc cttacctctt gtttgatgac acggtcaata
    36001 agcgcaaccc tcatgccaaa aaaggcagaa tcgtcatgtc taacctgtgt tctgaaatcg
    36061 ctcaagtcag caccccaagc actttcaaag aagatttgag ttttgaaacc attggagaag
    36121 atatctgctg taatttaggg tctatcaata ttgcccaggc tatggcagat gcgccacact
    36181 ttgaacagct catcacaacg agcatcagag cacttgatcg tgtctctaga gtgtctgacc
    36241 tgaattgtgc gccttctgtt gaggcaggca atgcagccaa ccatgcagta ggattaggcg
    36301 ccatgaacct acatgggttt ttggcaacca accacatcta ctatgataca aaagaagccg
    36361 ttgactttac agatctgttt ttccatgcca tggcctatta tgcttttaaa gcctcttgcc
    36421 aattagccaa agaaaaaggc gcttttgcag gattttcgtt atcaacctat gcagatggca
    36481 cttattttgc caagtatttg caagaagatg ccaagccaca aacagccaag gttgccacat
    36541 tgttgcaaga ttacggcttc acattgccaa cggtggcgga ttggcaagcc ctagtagcag
    36601 atatcaagca gtttggccta gccaatgcac acttgctagc ggttgctccg acaggctcca
    36661 tcagctactt atcatcctgc actccgagtc tacaaccagt cgttgctcct gttgaggtcc
    36721 gaaaagaagg tagcctgggc aggatttacg tccctgctta tcagattgac caggctaact
    36781 atgcttacta tgaacgtggt gcctatgagg tcggcccaaa agccatcatc gacgttgtcg
    36841 ctgctgccca aaagcacgtt gaccaagcca tctcattgac cctgtttatg accgataaag
    36901 cgaccacacg tgaccttaac cgctcctata tccaagcttt taaacaaaac tgtgcatcga
    36961 tctattatgt gcgagtgagg caagacgtcc tagcaggcag cgaacagtat gacgaggata
    37021 gtcttgtgac agcttctgga gctagtgatg agaccaccac agagtgtcaa agctgtatga
    37081 tctaacagct actagaaagc tctcgtgtcc cctatcacat gcataggatc agtgcgcacg
    37141 acctttcaag acaagcaagc atcagctctt gcttgtcttt ttttggcctc aaagcccgtt
    37201 agtctgctgc tatgcgaggc ttttttttga gcatcagaac ctcaaaaaaa aggacatgga
    37261 gtcctttttt ggtgatcggt gttgaggccg tcacaaactg cccttgaagc acgcttctat
    37321 gtggagcttt ttgtggtcct gtgacacgta agctccaacg acctccagct gacagcctct
    37381 tggaaacaag agctcaacct ctgaaggcac agccgaataa ggctcgacaa aggctgcctt
    37441 ggcccctttt ttgacacaga tgcgcacctc caccggtcga tgggtcatgg cgccgttttt
    37501 caagactgtc gtgctcataa aactgtgctt ggtgtagcgt gtaccaagct tgatcttaca
    37561 aaggatatga gggtcaaact gatggttacg atagtaagac gtgatatcag tgtgtgaaac
    37621 accgatatca cgcaaaaacg tctcgtatac atagcggtat actacaatgt tccacgggat
    37681 gactagccgg tgagtcgcag catcgagctg ggccacctga tcccttagct caggcgtgag
    37741 ttggctgagc tcacctttgg ccttgtctag gctggtgttg atagggcctg cctgctgaca
    37801 ggtataagca gccatcgcct cttgctcagc ccttgggtag atgagaccct tgccccagcg
    37861 ctttttaatc agtgtctcag cgcccttttg atccgaaaag gtaaaataag caggcgtccc
    37921 ctcacaaacc gcccgctcat agtgctcaca gacaaaaagc tcgaccaagc cctcagtttg
    37981 ccagatcaga ctaaaacagc taagaagaag gacaatagcc agttgatagc gcttttttag
    38041 catagttccc cctgacacca gcccaaaagg ctgacccttc ccctaggcct tttggctagc
    38101 tcatgatacc accagtttct cagacaggct aatctttttg tcatctcttt ctccctaaac
    38161 agactctctt atcatgatat tatgctatta tgagcgattt gtcaatccat agcaatcgtt
    38221 ttttagttag ataagtaagc ttttgaccta gaggcagaca agagtggcag tacctcccgt
    38281 tcactgtggt ggctaataag gactaacaca aaatctcaaa cgctttcaaa aacagctcct
    38341 gagcttaaaa aaggctgatg aacaattttt ttgagtttaa gagagattgc catgattttc
    38401 taatca
//
LOCUS       DAUGFP010000014        36372 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.014, whole genome shotgun sequence.
ACCESSION   DAUGFP010000014 DAUGFP010000000
VERSION     DAUGFP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36372
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.014"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..241
                     /locus_tag="VH663_000721"
     CDS             <1..241
                     /locus_tag="VH663_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ8173940.1"
                     /translation="LIDLPKLKKDIEELNHLKDDYNKAIKLNYLDKKNEDSLGMLMDN
                     LDIRKSSYDVQSRIVKQLIDRVEVTMDNIDIIFKF"
     gene            complement(418..1329)
                     /gene="whiA"
                     /locus_tag="VH663_000722"
     CDS             complement(418..1329)
                     /gene="whiA"
                     /locus_tag="VH663_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEQ8173941.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSFIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFDLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYLQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            complement(1326..2303)
                     /locus_tag="VH663_000723"
     CDS             complement(1326..2303)
                     /locus_tag="VH663_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEQ8173942.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLAPPGDLRNVLLAMSDMPKFYERVFQYRFNENDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYQGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            complement(2300..3190)
                     /gene="rapZ"
                     /locus_tag="VH663_000724"
     CDS             complement(2300..3190)
                     /gene="rapZ"
                     /locus_tag="VH663_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEQ8173943.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYQVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            complement(3605..4951)
                     /gene="asnS"
                     /locus_tag="VH663_000725"
     CDS             complement(3605..4951)
                     /gene="asnS"
                     /locus_tag="VH663_000725"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEQ8173944.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVTNKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVTKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            complement(4972..6165)
                     /locus_tag="VH663_000726"
     CDS             complement(4972..6165)
                     /locus_tag="VH663_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ8173945.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            complement(6500..9001)
                     /locus_tag="VH663_000727"
     CDS             complement(6500..9001)
                     /locus_tag="VH663_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEQ8173946.1"
                     /translation="MFCFIDIACYNRLTMIQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLSHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLVYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGSKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRVQDDKDFTR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRV
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRKDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            complement(9109..9873)
                     /locus_tag="VH663_000728"
     CDS             complement(9109..9873)
                     /locus_tag="VH663_000728"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEQ8173947.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHVDGFKIGILDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            10041..10739
                     /locus_tag="VH663_000729"
     CDS             10041..10739
                     /locus_tag="VH663_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ8173948.1"
                     /translation="MPFIFRYSFFNKVLIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            complement(11049..11978)
                     /locus_tag="VH663_000730"
     CDS             complement(11049..11978)
                     /locus_tag="VH663_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173949.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(11971..12663)
                     /gene="ftsE"
                     /locus_tag="VH663_000731"
     CDS             complement(11971..12663)
                     /gene="ftsE"
                     /locus_tag="VH663_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEQ8173950.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            complement(12682..13780)
                     /gene="prfB"
                     /locus_tag="VH663_000732"
     CDS             complement(join(12682..13707,13709..13780))
                     /gene="prfB"
                     /locus_tag="VH663_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEQ8173951.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            complement(13849..14973)
                     /gene="queG"
                     /locus_tag="VH663_000733"
     CDS             complement(13849..14973)
                     /gene="queG"
                     /locus_tag="VH663_000733"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEQ8173952.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERHLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNSPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILAFMSHLTLKNEDSRKELELIRHKWQV"
     gene            complement(15669..16318)
                     /locus_tag="VH663_000734"
                     /pseudo
     CDS             complement(15669..16318)
                     /locus_tag="VH663_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            complement(16615..17250)
                     /locus_tag="VH663_000735"
     CDS             complement(16615..17250)
                     /locus_tag="VH663_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEQ8173953.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLPQVEVMV
                     TGGISLDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            complement(17255..18256)
                     /locus_tag="VH663_000736"
     CDS             complement(17255..18256)
                     /locus_tag="VH663_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEQ8173954.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEVNIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMTLTAWQGNRVGLYYLENGFGCRSSQV
                     YYDRCGSSFSALDKDSLDLAAIFEGISHFHFSGISLALGKKTQDLIEVLVREAKKRDI
                     CVSFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADATMI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLANQRDVQR"
     gene            complement(18285..18926)
                     /locus_tag="VH663_000737"
     CDS             complement(18285..18926)
                     /locus_tag="VH663_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEQ8173955.1"
                     /translation="MKIALINENSQAAKNGIIYDALTTVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKGRAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVTYLKSVLEQ"
     gene            complement(18951..19745)
                     /locus_tag="VH663_000738"
     CDS             complement(18951..19745)
                     /locus_tag="VH663_000738"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEQ8173956.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGLAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            20069..20506
                     /locus_tag="VH663_000739"
     CDS             20069..20506
                     /locus_tag="VH663_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ8173957.1"
                     /translation="MIAIIVMGHGHFASGIVSALELIAGKQEKVTAIDFTAEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVLGLITCSKEGIVDWKTLSQQEDGTTDDELGGI"
     gene            20506..21705
                     /locus_tag="VH663_000740"
     CDS             20506..21705
                     /locus_tag="VH663_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEQ8173958.1"
                     /translation="MARSLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYSGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTSEAREAALKAADKLVQRYQDKGGFIQ
                     AWGVLGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPVKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFLNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDHDKKTYE
                     AAMHSMLRALIKDYANKDLNPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            21741..22229
                     /locus_tag="VH663_000741"
     CDS             21741..22229
                     /locus_tag="VH663_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ8173959.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKYLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQSIFIVVKDLQDAKHLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAICCLAHDHQVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            22248..23030
                     /locus_tag="VH663_000742"
     CDS             22248..23030
                     /locus_tag="VH663_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ8173960.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGIILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVAIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWSFAILGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            23017..23838
                     /locus_tag="VH663_000743"
     CDS             23017..23838
                     /locus_tag="VH663_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ8173961.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAVVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVLLTVLIGIIGSWLGILA"
     gene            23925..25832
                     /locus_tag="VH663_000744"
     CDS             23925..25832
                     /locus_tag="VH663_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEQ8173962.1"
                     /translation="MSLAFTRFKETVNPDFCRNYLLDYQADSYADRKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQKAVTDDPEWNFMLNRQTYLQTLILVYLVERDERYL
                     LKAKSFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLFNALTKQEESLIL
                     ASLEKQLQFLHTNYLDKYSLSNWGILQTTAILLADAYFGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAAFPALDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSICLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQINSSWAYLSYPKSNYCHLRQNGHVYF
                     IEGSYQTQFSDRNNYQHDRQILILPPGMFLIIDTIQAQGNHCLVSQFILDNHLEVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKLIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALAWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            25896..26891
                     /locus_tag="VH663_000745"
     CDS             25896..26891
                     /locus_tag="VH663_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ8173963.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGKFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(26975..29656)
                     /locus_tag="VH663_000746"
     CDS             complement(26975..29656)
                     /locus_tag="VH663_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEQ8173964.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTMLVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTH
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(29886..30272)
                     /locus_tag="VH663_000747"
     CDS             complement(29886..30272)
                     /locus_tag="VH663_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEQ8173965.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            30354..31655
                     /locus_tag="VH663_000748"
     CDS             30354..31655
                     /locus_tag="VH663_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ8173966.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEALFHTDHEAFTQEIITNPETEINAILVRHAPDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSRGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMMQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            32111..32920
                     /gene="yidA"
                     /locus_tag="VH663_000749"
     CDS             32111..32920
                     /gene="yidA"
                     /locus_tag="VH663_000749"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEQ8173967.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHVVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFLSRK
                     LGVHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            32920..34146
                     /locus_tag="VH663_000750"
     CDS             32920..34146
                     /locus_tag="VH663_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEQ8173968.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLGIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            34133..35368
                     /locus_tag="VH663_000751"
     CDS             34133..35368
                     /locus_tag="VH663_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ8173969.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(35467..36225)
                     /locus_tag="VH663_000752"
     CDS             complement(35467..36225)
                     /locus_tag="VH663_000752"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEQ8173970.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
BASE COUNT        11218 a         7766 c         6413 g        10975 t
ORIGIN      
        1 tttgattgac ctaccaaaac taaaaaaaga catcgaagaa ctcaatcatt taaaagatga
       61 ttataacaaa gcaattaaat tgaattattt agacaagaaa aacgaagact ctcttggaat
      121 gctgatggat aaccttgaca ttagaaaatc ttcgtatgat gttcaatcta ggatagttaa
      181 gcaattgata gatagagtcg aggttactat ggataacatc gatattattt ttaagtttta
      241 aaagcattat ttaggcacta caactagtat agttgtacat gccattatcg gtctccttta
      301 caaacgttat tatccctttt atcatatcac agaacatcag acacttcaaa aaaacaatgg
      361 aaaacgactt ttagacagaa aaatcgtatg aacaaaggct catacgataa aaatgttcta
      421 taagtcatct gcaattttat tgattttacg caagcgatgg ttgaccccgc tcttggtgat
      481 tgggaactca agggcatcag caacttgttg aatggaatag tctggatgct taacccgaag
      541 ctgggctacc tgttgcaatt caatcggtaa actttctaaa ccaattgtgt ccatgatttt
      601 aataatatta ttaatggttt tcatgctagc actgatagtt ttggcaatat tagctgtttc
      661 tgcattattg gcacggttaa tatcatttcg agcttctcga agtaatttga tggcttcaaa
      721 gtcttctttg caggacatgg caccaattat aattaaaaag tccatgatat cttctgcctt
      781 ttgcaaataa gtaactgcac cgcttttatg ctcaatggtt ttggcatcta gcataaattt
      841 ttgcatgagt tgagctaaat cttgagcatg atcaagataa actgaataga tttctaactg
      901 atacttgcct gactcaggat ctctgatgct acctgccgct agaaaagccc ctttaagata
      961 agaacggcca gcattatcgt ctgataaaac ctgaggttcg atgcctgttt ccagatcaaa
     1021 aaaggagtcc gctaatttca aatcggctaa aatggtttcc acaccttgct caacatagac
     1081 ggtgtaaaca cggtttttgc gcaggttcgt cttttgatga tacctgattt ctggcacgat
     1141 aacataggca tcttcaataa aagaataaat gtagcgggca attttagcat tttcagtggt
     1201 aatggataaa tgaaggcttt ggtgagctaa gcccaagctg cctgataatt tgatgatggc
     1261 ggccaattca ttgttgtcac ctgtggacaa gtgaattaat tcctctttga ccttggttgt
     1321 aaaactcatt gcttgactat cctcactaaa ttcattaatt cttccaccac taagttgcca
     1381 tcgtgaaaag ctccgccatt ctccaaccgt aaaaaataag acgaaatcac acgcttagca
     1441 gccctgcaaa ggccagcaaa gtcgtgatca acctgaacga ggtattcatc aaatttgtta
     1501 gaattcatgt aagcctgcgg caccttagcc acatttacca aaaccgtatc aatcaaatct
     1561 cttcctaagt gctgatttaa aacagccaca tggtctgcat ctgaaaattg ttctgtttca
     1621 ccatactggg tcatgatatt acaaatatac accacttctg ctttagtctg gcgcaaagct
     1681 tctttaattt ctggaatgac caaattaggt aaaatagagg taaacagtga gcctggtccc
     1741 aagacaatca tgtcactttc tagaatagcc tcaaccacct tacgactggc ctgaggcttt
     1801 tggtcgttgt aggtgttggt cacatagaca tggtcaatca tgccttggta tttggcaata
     1861 gaactttctc ctgctacttc atgcccatct ttaaaaacag catgaagggt caaggcctgc
     1921 tcgctagagg ggtaaatttt acctgtaata tggaaaaatt tggttaaaat ttgaatggca
     1981 ttgtaggttg agccttgcat ttcagagata ccagcaataa tgagattgcc tagagggtga
     2041 ccagccaagg ctccatcatt ctcgttaaaa cggtattgga aaacccgctc ataaaatttt
     2101 ggcatgtcac tcatcgctaa caagacattc cgtaagtctc ctggcggggc taattgcatg
     2161 gcattacgaa gttctcctga ggaaccacca tcatctgcaa cagtgaccac agcagtaata
     2221 tcaactgctt cattacgaag gctttttagg ataatggaga tgccagtacc cccacctata
     2281 acagtcattt taggattttt catgaacgat tcaccgtttc cttacgacga ttttgatcac
     2341 gatggctttc attaaccgac caatctgttg ccagactttc tgccaagcaa tgggcaaagg
     2401 caacgctgcg gtgttggcct cctgtgcagc cgatagccac cgtcaagaca gacttccctt
     2461 ctttttggta agccggtaag atagggacaa taaggtttaa caaatgcttg taaaacacct
     2521 ctgattctgg gtgagacatc acataattaa aaacgtcctc atctagtcct gttttttcac
     2581 gaagctctac ctgataataa ggattgggta gaaaacgcac atcaaaaacc aaatccgcat
     2641 ccaaaggaag accatatttg aacccaaagc tcatcacttc aatacggaaa gaggcttgat
     2701 tagacccttc agaaaactgg tctgaaatgg ttttacgcaa ttgtctaggg gtcaattttg
     2761 ttgtatccac cacatgttgg ctcatgcttt tcaaaggaga taggagttct ctttccaatc
     2821 gaataccatc aagcacacga ccgtccgcag ccaaagggtg gctccgtctg gtttctttat
     2881 agcgtgacac caattctcca tccgttgcat ccaaaaaaag aatccgaaaa tcaatgctag
     2941 gattgctttc aatactatct aaggtagaat taatttcctt gaaaaacaaa cgacttctca
     3001 tatcgacaac caaagccacc ctacgatttt cattggtttg ttcaattaat tctaaaaatt
     3061 ttggaaccaa ggctgggggc atattatcaa tggtaaagta gcctagatcc tcaaaagact
     3121 gaatggcaac tgtttttcca gcgccgctca ttcctgtcac aataactaaa ttaatgtgtt
     3181 tgtctgacat ttacaatttc cctttctcta tcgaaccgtc caccttcatt ataccacata
     3241 cataagaaag ctaagaagtg aaaaagtggt aacacatttt gaactgtaaa aatcagctaa
     3301 ttttaataat aactcccgta gctctcctat tatatgaata gacgagacct ttttagtatc
     3361 aaatgagagt gtctatttga taatcgtagt ccaaatactt gtctataaaa tatcccttga
     3421 gcaaggctat attgtcttat ctgctaactg atctactaaa cttccaaaat ttatcttcac
     3481 aaaggatgta ggttcaaact ctactatttc ttatctctcc tttaggacat attgatgttt
     3541 ttataacaca ctcacttctt accaatacaa aaagttcata gatgacatga acttttttgt
     3601 attattaggg tctaatccga tgtaacatac gtgggaatgg aatagcttcg cgaatatgtt
     3661 tggtaccagc aacaaaagtt accatacgtt caatacctat accaaaaccg ccatgaggaa
     3721 cagagccata tttacgaagg tcaaggtaaa agtcatattc tgatttatcc atacctagtt
     3781 catccatttt agtcacaaga gcatcgtagt tgtcttcacg cattgagccg ccaatgatct
     3841 ctccataacc ttctggagct aaaagatcag cacaaagaac gcgttctgga tttccaggaa
     3901 ctggtttcat ataaaatgct ttaaagcttg ctgggtaatt cactacaaat gtcggtactc
     3961 caaagtaatt agaaatccaa gtttcatgtg gggaaccaaa gtcatcacca tgctctaaat
     4021 gttcataatc tgtatcttca tctgcttcat gctcttgaag aagtgtaata gcatcatcat
     4081 aagaaacacg cttgaatggc tctgtgatat aacgtttgag agcctcaaca tcacgttcta
     4141 aaatatcaag tgcttgaggc gcacgatcta gaacgccttg gatcaatgct ttaacataag
     4201 cttcttgaag gtcaagtgat tcctcatgtg ataagaaaga atactcagca tccatcatcc
     4261 aaaattctgt taaatggcga cgtgttttag atttttcagc acgaaagact ggaccaaagt
     4321 cgaaaactcg gcctagagcc attgctccag cttcaaggta taattgacca gattggctaa
     4381 gaaatgctgg tttcccaaaa tagtctgttt cgaataactc tgttgaatct tctgctgcat
     4441 tttctgataa aattgggcta tcaaatttga taaaaccatt ttggtcaaag aattcataag
     4501 ttgcataaat aatagcgtta cgaatttgca taacagccat ttgtttacga gaacgcaacc
     4561 aaaggtgacg gttgtccatc aagaagtctg tcccatgctc tttaggagtt ataggatatt
     4621 catgtgactc tccaatgatt tcaaggtcag taatatcaag ctcatagcca aatttagaac
     4681 gctcatcctc tttaacaatc cctgttacat aaacagatgt ttcttgattg agacgtttaa
     4741 taacgtcaaa tttttcaaga cctgattctt caccatattt ttcaatgaag tttggtttaa
     4801 aagcgacacc ttggaaaaaa gcagaaccat cgcgcaactg tacaaacgca atcttacctt
     4861 tacctgattt gttagtaacc caagcaccaa tagtaacttc ttgaccaaca taatctttaa
     4921 catcaacaat tgaaataagt ttttttgaca taagtttctc tctattttta tttaattaat
     4981 ttcattactt tccataaaga catgcagtct acgaacagct tctttaaggg tttcgatatc
     5041 tgtagcataa cttagacgaa cattttccgg tgcgccaaag cctgctccac tcacaacagc
     5101 taatccgact tcctctaaaa tagcattggc aaagcttgtc acatcggaaa aacctgtcat
     5161 ttccatggct ttcttaacgt ttggaaagaa gtaaaaagct ccttgtggtt taacaacctc
     5221 aaacccaggg acctgacaca acaatggata agtgatatta aggcgctctt caaatgctaa
     5281 tcgcatctct tcaagtgatg actgactacc acagaaagcc tcgatagcag catattgtga
     5341 aacggttgtc aggttagagg tagtctgccc gatgatctta ctcattgccg aaatgatctc
     5401 tggttcacct gctgcaaaac caacccgcca gcctgtcatg gcataacttt tagccacgcc
     5461 attgaccgta atggtttgac gacgaatcgc ttctgagagt gttgaaatag gaacaaactg
     5521 attgccgtta tagactaagg acccgtaaat atcatctgcc agaataagaa tatcgttgtg
     5581 caccgcccat tctccaatgg cccttaattc ctcagcacca taaatcatgc ctgttggatt
     5641 ggaaggagaa ttgataagga caactttagt cttacttgtc cttgctcttt ccagctggtc
     5701 aacagttacc ttgaattgat tttcttctag tccttgaacg aagatcggct gaccttctgc
     5761 catttttact tggtcagaat aagataccca atatggtgtt ggaatgagaa cttggtcacc
     5821 aggatttaaa acagccataa aaaaggcata aaggatgaac ttggcacctg ttcctgccac
     5881 aatctgatca ggagacaagt gatacccata ctgatttttc agatatgtgg caatcgcagc
     5941 ttttaattct ggaagcccag aagcatttgt ataaaagcta gccgtaccat tttgaataga
     6001 ttcaatcgcc ttgtcttgaa tgtgcttagg tgtaaaaaaa tctggttctc ctaaagttaa
     6061 attcagtaca tcacggccct gcgctttcaa agccttagct ctagcacctg cagccagagt
     6121 aacactctct ttcatttcta aaactcgctt tgatagtttg ggcatcgtat ctccttttta
     6181 tcgacttctt atcttcagat taattaatga tcaacagatc attttttcat aatctacggt
     6241 ttcagtttta ccaatactcg ctactaatcg cacgaactat gacaagctgt tttgatagca
     6301 actgacacta atgaaaagaa cagtaattgg gtttactaaa accaaaaatt tgttcattta
     6361 ttgtccttat caaaaaaaca attcgaaaac atttgaacag ttgtctaaaa gagcataata
     6421 aaagttcaaa ctaatttaaa tgttagttga ttgtgcttaa cgccattaaa aatttttctg
     6481 atataataga tcctttctat tatatcaaaa aacagtccgt ttctactaga caatcgtgaa
     6541 aattttgttg agaaatcaaa aattcctgac cgagaccttc caaaataact tgtccatagg
     6601 actttgtaag gagacgacga tccaaaataa taaccactga cttttgatct tttcgacgca
     6661 tcgtgcgacc aatagcctgt tttaatcgca aaatagtcat tggcaagaag taatctctga
     6721 aaggattttt acctttttcc aatagataat gactcatttt cttaacaaag aaatcctctg
     6781 ggttatcaaa gggcaatcgt gcgataaggg taatcatacg atccgcttga ataaaatcaa
     6841 ctccttccca aaaagaacct aaaccaagca aaatagtttg ctctccttga tcaaaccgtt
     6901 ttttaatatt ataagctgtt ccattctttt cctgcgctaa gtgaggcacc tgccattggt
     6961 ctagatagtc tgaaaccaac agcaagtgtt ttttagaatt aaaaagcact aaaataggat
     7021 aaccctcttg cttcaagctt tcgattcgtt ttgcaattgc ttccacatag atttggtcac
     7081 tgacttctgt cacgataggc gcctcttgat caacaagcac caactggtct tgctttttat
     7141 ccttttcaat gacatgatag agatactctt caaaacctaa taggtctgcc aaggttactt
     7201 ctgagctgat ggttaaagtg gcagaaacaa aataagtttt caccgtttct ggtaagagct
     7261 gttggaaatg aataaatgcc ttactagcac tgtttagata agtcactcgc ttctcctctt
     7321 gcttttcgct actaagccag tagtcaccat ctgaggtagc aaaaaaggct tgtaactctt
     7381 gataatctgc tcctgttaat tctttggcgt agccagctat acgagaccaa tcatgagcaa
     7441 gctggtgctc tttattttga tagtaatcac ttgacacttg tcccagttca aacgacaagc
     7501 tttccaataa gcgtttttct aaaagaggta aagggttgct taacttggcc tgtattgttt
     7561 gtaaaaaaac cgtcaggtta agctgatgac gtgataattg atctaattgg agcatcagct
     7621 tttgggcctc atcaaagacc aagaccttgt ttctagtaaa gtccttgtca tcttgaacac
     7681 ggtgtaaaaa ataagcatgg ttggtaatca ggagacgggc tgttttggct ttttcataac
     7741 taactctcca aaaatcataa tcgtaaaatt cagaagactg cttaatgtct ccatcgtgct
     7801 taagctgctc aaaataagcg gcaaagcgct gcttttgttt gatttcgtca aggtctcctg
     7861 tcttagtttc taagagccaa accaataatt gcattttata acgattcacc aaacggttct
     7921 ggtcattttg gtccaaacta tctgcaaagc tatctagctt taggtaattg gcaggccctt
     7981 ttaaactatg gcaggcgatg tgaaattgtt cttgaatagc agctacttca ccagccatta
     8041 attggtcttg aagaagtttg gttggcacac taacaatgat ttggttttga tcttcttttg
     8101 ccaaaagggg tagcaggtag ccgtaggttt ttccaatacc tgtctgagct tcaataaaac
     8161 tagctactcc ctgatggtaa tcttcgtcaa ttaactgagc aaattgagtc tgtttaggcc
     8221 tttcttccag acctaatagt gccatattaa tagggaagct ttttgatatt tgataaggct
     8281 tgagagcttt tgagcccttt ggtaagagaa tctgtctgat tttaatgtat ttgttgggat
     8341 cataaggttt ggctttggct aatccttctt gaatgaccat tgctgtttca aacagcaggc
     8401 tatctgaata gactaataag ctttctaagc actcaattgg aaggctctca attttttgta
     8461 aaagtcttaa aaacagtata gctgtagctc ttgcatctgc aatagcagta tgagcttctg
     8521 ccaaatcaat attgagctgc ctagataggt gactaaggtt atacttttca aagcgaggga
     8581 aaaatagttg ggctaattca acagtatcaa cacgtggtat tgttaactca tatccttcca
     8641 aaaaaagagc ttctgccaat aaatttgcat cgaactttac attgtgggca acaaaaatac
     8701 aatcttcaat caattgataa atgtgatgcg ccacttgacc aaaatccgga gctttagcca
     8761 actgcttatc cgtaatacct gtcaaatgaa caatatgctc atctaaagat tcatgaggat
     8821 taacatctgt ttcataacta tcaataattt tatttccttg gataataaca atcccaacct
     8881 gaataataga agcattggga cctgctccag tagcttccaa gtccactact gcatattttc
     8941 taagtttttt ctggatcata gtaagtctat tatagcacgc tatatctata aaacaaaaca
     9001 tgactatatc tcaaaggtgc catcactcca ataaccaaga taggataatt ttttaagaag
     9061 tataaaaaac actgtgagtt tcactcacag tgtcagcatt aatcattttt aatggaatac
     9121 cataccacca tcgacaacta ttgtttgtcc agtgatataa tttgaatcat ctcccgctag
     9181 gaagcctact gcattagcaa catcttctgg ttcagacaag cgtttcaaag tgatatcttt
     9241 tgcaaattgc tccatacccc attcatcatc tttgcctgca tttttaccaa cttcatgggc
     9301 aatatcaaac atcataggtg ttttaacaat acctggtgcg taagcattga ctgtgattcc
     9361 ttctgatgcc aaatcctttg ccaaagtctg tgtaatacca cgaactgcaa attttgtacc
     9421 accataaaca gtcaaattag gattcccttc acatcctgct tgtgatgttg catttataat
     9481 ttttccgcca tgacctaatt ctctaaaatg tttttgagct gcttggcttc cccaaatcgt
     9541 tcctccgaca ttgattgcaa aagccttttc aaactgctct tcagtaatcg tgtcaagagg
     9601 ggttgttggg gcaacacctg cattgttaac aacaacattc aaatcaccaa aagtatctac
     9661 aactttttgg aaagcatcaa aaacttggtc acgttttgaa acatctgcta caactgcaac
     9721 agcatcctca ggtgatagtt tatcaaccgc agcctgagca gtctcttcat tatagtctaa
     9781 aatgcctatt ttaaagccgt ctacatgaag acgtttcgca atagcaaatc caattccttg
     9841 accagcacca gtaacaatag caacttttga catatcttcc acctcatatt ttttagtcaa
     9901 ccgattagat tgctctcaaa aagtcagtgt tcttaaacta agttcagctt taggcttggc
     9961 ttattgatta cttttatgat acacctttat gaaaacggtg tcaagaaatt gctgtgagat
    10021 ctcatatttt ctgaaactaa ttgccattta ttttccgcta ctcttttttt aacaaagtct
    10081 tgattttttg gtacactatc cttatgaaaa tctataaaac aataaaccac attgctggtg
    10141 aaaatactta ttacctcgtt aatgatcaag ctgttatcct cattgatcca ggaagtaacg
    10201 gccaagaaat cattgctaaa attaaatcct ttgaaaaacc tttagtggct atcctcttaa
    10261 cgcatactca ctatgatcat atttttagtt tagatttggt cagagatgct tttgatcatc
    10321 cacctgttta tgtttcagaa aaagaagccg cttggctatc ttccccagat gataatttat
    10381 ctgggttagg ccgtcatgat gatatcatta atgttatagc aagaccggca gagaactttt
    10441 ttaaattaaa gcaaccctat caactaaatg gttttgaatt cacagtttta ccaacacctg
    10501 gtcattcttg gggaggagtc tcatttgttt tccactcaga tgaattagtc gttactggcg
    10561 atgctctatt tcgtgaaact atcggtcgta ctgacttacc taccagtaac tttgaggacc
    10621 tcattactgg tattcgccaa gaactattca ccttacctaa tcattaccga gtttatcctg
    10681 ggcatggtcc ttctactacc atttgtcacg aaaaaaatgc taaccccttt tttcattgac
    10741 atagctattt tagatactgt ttgaggttat cctaaaactg aaatcatagt attttactaa
    10801 aaaagagcca ctaaattttg aatgacaccc caaaagttag atacatcaaa catctaactt
    10861 ttggggtgct tttcttctct tgaaacctta gaaactgcta ttttaaactg tattgcctat
    10921 tacaaataaa caaatgaatc aaaacaaaat gaaaaggacc agtcctgtgc aatacaaaac
    10981 gaggtccttg gaataatata agaggtccaa cttttgaggg tcgctacgtt tcgtcagcag
    11041 cgcttttttt agaattttaa ataacgtctc atagaaagca ctgaaccaag cgaaccaatc
    11101 ataataccta tgacaaacaa agcaccaatc aggtaatata cataaggatc aagcggatac
    11161 atcgagagat tattacgttg taattcttgt gcaaaatgct tataaacaag atcatatcca
    11221 taataaatca gaagagatgg taaaacagct cctaacaagc caacccaagc cccttcaaaa
    11281 aagaaaggcc ctcgaatata agaattttta gcaccaacta accgcataat ttcaatgtcg
    11341 cgtttacgag acataatagt catacgaata gtgtttgaaa tgagaaagac agctacaaat
    11401 aatagcattg ctgttccgat cagcccccaa gtttgaatta atgttgaaaa cttaaatagt
    11461 ttatcggagt taatccctcc ataatctgcg gcttctacgc cttcaatcgt tctgatcttt
    11521 ttagtgatcg ccttaacttg tttaggcgtc tgggtctcaa taagatagat atcttggagc
    11581 ggattagtgt cttggtcata catattccaa acatcaccta aggtttcttg caattttttc
    11641 aattgttcat ccttgctaga aaatgtaatc tttttgacac cttttatctg agcaattttg
    11701 tcgtaaacac tgtggtagtt atcgttatta acaggttcac cggcagtatt ttgaataact
    11761 tttgcagcat cagttgaatc aacttgtaag taagtattga tatggacatt attttcaaca
    11821 cccgaagcaa cacgctgtat attcaacaat gtcgccgcaa aaacaccaac taaagtcaat
    11881 gtaacagcca ccatactaac tgaagcaaat gtcatccaaa agttgcgttt aagattttta
    11941 atggattccc aaatatgacg gaaaaagtat ctaatcatcg taaccataat ctcctttttc
    12001 ttcatcacgt actatgcgac catcttcaat agcaacaaca cgatgacgga atgtattcac
    12061 aatatgactg ttgtgtgtcg ccattaagat agttgttccc tgaacattaa tacgttcaag
    12121 taactgcata atttcccatg aaatctcagg atctaaattc cctgtcggtt catctgcaat
    12181 caataattta gggttgttaa caatcgcacg cgcaatggcc acacgttgct gctcccctcc
    12241 tgatagctga cttggaaaag aacgcatctt atgtttcaag ccaactaaat cgagtacctc
    12301 tggcacgcgt tttttaatgt ggcggcgctt ttcaccaata acttccatgg cgtaagctac
    12361 attttcaaag actgtttttc ttggtagcag tttgtaatct tgaaaaacta ctccgatgtg
    12421 acgacgaaga atcggaacat cacgtgcttt tagtttagtt aaattgaatt cacccacata
    12481 tagcttacca gttgtcagtt gttcttcacg gtaaaggagt ttgataaaag ttgatttacc
    12541 agcacctgat gggcctacga gataaacaaa ctcaccttgg ttaacagaaa cattgacgtc
    12601 tctgagggcg gtcgttgagc ggcggtattt tttagtaaca ccagacattt ctattagtgc
    12661 catgtttctc ctttattatt tttagtcttc aatgcgccac ttaaggtagg catcaataaa
    12721 gccattgatt tctccatcca taaccttatc tacctgagca agctcaaagt tagtacgatg
    12781 atccttcacc atcgtataag gggtgaaaac ataagaacga atctgacttc cccaagtgat
    12841 ttctttttta tcacccttga gagcatctac ttcttgtgct ttcttttctt gttctaactg
    12901 atatagctta gcctgcaaca tcttcatagc gcgatcgcgg tttccatatt gagtacggtc
    12961 aacggtagac gataccacaa ttccagttgg aatatgcgtc aaacgaacac ctgttgatac
    13021 tttattgaca ttctgaccac cagctcctcc ggaacggaaa gtatccattt taatgtcatc
    13081 atcacgtact tctacttcaa tcgtatcatc taactctggc atcacttcaa ctgaagcaaa
    13141 tgaggtatga cgacgtttag ccgaatcaaa aggtgatata cgaacgaggc gatgaactcc
    13201 catttctgat ttcaacaaac cataagcatt aggaccttca aaagagaggg taaccgattt
    13261 aataccagct tcatctcctg cttgataatc caatacttct actttgaaac cattagcatt
    13321 gccaaaacga gtatacattc gtaagagtaa atctccccaa tcttgcgctt cagttcctcc
    13381 tgaaccagga tggatttcta agatagcatt attgtggtca tagggttcag ataagagaag
    13441 cgtcatttca taagcaccca ttatcttatc tagctgcatt agattttctt ctaattcttc
    13501 tttcaaagaa tcatcttcgt caagcatctc aagtaagagt tctgtttcgt cagataattc
    13561 ttgcatatta tggaaagtat catacttacc ttttaactca tttagttctt gagatgtctt
    13621 ttgcgctgca atattgtcat tccaaaagtc tggctcagtc atgtgatttt caagaagagc
    13681 aatctcctct tccaaacgat ctaagtcaaa gagacctcct gaagctagtc aacttctctt
    13741 tattttctac tattttttgg cgaatttcag ccacttccat gagatttctc caatctttgt
    13801 taatctgttt tattttatca taaggttctt aaaaatacca taaaaggctt aaacttgcca
    13861 tttatgacgg attaattcca actcttttcg gctatcttca ttttttaagg ttaaatgact
    13921 cataaacgcc agtatctcat cgtttggttt tttgacaatt tctcccaatg cccatatagc
    13981 tgttgcagta tgaatagggt tattattttt atcaataatc tctattaatt tgaccactgc
    14041 ggttttatca tgagcatttg ctagcgcaat aatggcatta cgctgtaaaa tatttttacc
    14101 tcgccaactt ccagcaatca taccaaattt ttctttaaat tgtccatttg ataaactcaa
    14161 aaatggtatt aattctggtt gagccaactc aggatcaatc tctgtagctg gtgaattgtt
    14221 tatacccttg ttataaggac agcaaatttg gcaaatatca caaccataga taactgtttt
    14281 aatctttttt cgaaactcta tgtccatcat tcccttatct tgggtttgaa atgatagaca
    14341 acgcttggca ttcattgagc catcaccaat taaacaagaa gttggacagg catctagaca
    14401 acgacgacag tcaccacaat cataatcgac aggcttgtct ggctcgatct ctaaattggt
    14461 aattaattct cccaaaaaca tataagagcc atattcttta gaaataacta gaccattttt
    14521 tcctataaaa ccaatacccg ctctttgggc aactgcagta tcaactaaag ctccagtatc
    14581 gaccattgct ttttgttgca aaggaaaatc tcgacacagc tcttcaatgc cttttgatag
    14641 tctgtctaac ttttcgccga ccacataatg ataatcaagc ccccacgaac taggtgtaat
    14701 ttttccacgt ttataaggtg ttttttgagg ttgctgagga agtttgtgag gataagcaac
    14761 gccaattgaa ataatcgttt tagccgattc taaatgcctt tcagggtaga tacgatcctc
    14821 tatgacttta tgttcaaatc ctgaattgcg cccctcttcc acagaagcac gcaatgactt
    14881 ttccaaataa tcaaagttat ctgccgtcgt aaaaccaatt ttagagatac caatttcttt
    14941 ggcgagtgcc ttaatttctg ccttaatagt catactacta tcataatcaa aataagttaa
    15001 gacttcaagg aaaaagaagg acaattctaa tcaaacataa ccattttact tcctgattta
    15061 tttggggttt ctctcttaac atgcacaaga atgcaacaag taaattcaat tacatcgtaa
    15121 aagtcccttt tctggttacc tctacttggt cagaaatcac atctccatca cgtcttgtct
    15181 agacagtcag ctcagctcta aagtaagtct gactataagc aatggaccta cgaagacaag
    15241 cagcataaca cttccaaata ttattatacc taaccaaaac cgagtcacat tacccgtgca
    15301 actcatggac tcagatactg cctttgtcaa agcagtatta tgatccttct atatttactc
    15361 actagccaat ctaaaaacaa atccaaataa tgaccacggt taaacatatc tcctgccaca
    15421 taaagatggt caacgactaa cgatcgcatc caaatcacaa aaatcataat ccttttctag
    15481 gtgatcttat gcgacggttt ttttgataat aacttaaaca tagtactctt ttcagtgtca
    15541 tattgtaaat aggtagctaa aaaacagtaa aaacacataa atatattctt gttattggtc
    15601 tgtagaaagt cctagacgta ttcttaagtg tcttgaaata tcatcaatga tcagttagtt
    15661 gtgttacttc acgatccaaa acaaccgatt tttacacaca gttgtcctaa atgatctatc
    15721 ttgtgatcag cttgactctg atcaatgcca tatcggtagt cggtaatggc aaaaactgtc
    15781 agattggctg ctttggcggc agcaatgcct ttttggctgt cttctactac aagcagttgt
    15841 ttttttgtaa tcctagtttt tgtactgctt tattatagat atctggataa ggtttgcctc
    15901 tagaaacatc ttcacgcgct aggattattt caaaataatc ttttatttgc gttgtctcca
    15961 acgccaacaa aacatcctga cgcttcgagt ttgatgccac agccagttta ataccttgtt
    16021 ttttcaactg ttcaagacaa gagttcactt ctgtaatcaa cagtttttga taaggaggct
    16081 tatgcgcttg tttgtaagcg tcatagtcag ttgtaattgc cttaacgata gcatcatccc
    16141 tatttttacc taacaactct ttccataatt cttgaagatt gcccccaata aaatctttag
    16201 agttcaaatg atcaatggga attccctttg tcttaaaaaa atcttctcgt cgcctcagat
    16261 aaaaaggttc tgtatcaaat aaaacaccgt ccatatcaaa aataattcct ttaatcatca
    16321 acatttctcc ttttttctat tttagcataa gttgctgtaa aagctgtaca gctagtccat
    16381 tctaagtgat atcactttag atcaggtcca tactaacgaa ttcaaaaggt attacttttg
    16441 tttgatcata aaaagtttat atcctatata tcaaacgaca ggctcatctt gttaaattag
    16501 ctgctcttat atctatatta tctggaaaaa cataagcaga agcatattaa ttgaatgaat
    16561 gataaaaaaa acgtttttac tagcactaaa gccaaggtaa aaacgttttt tagattattg
    16621 tcgcaaactt ttgatataat gagcggcttt cttagtgatt aggttatatt gtttttggct
    16681 cgctaactga ttaaactcgc ctcctatgcc tagaacatct actccagcag ctagccagtc
    16741 tttgagatta tctaaagaaa tcccacctgt aaccataacc tctacttgtg gcaaaggaga
    16801 tttgattgcc cgaataaagg acatccctac agtagaacca ggaaaaattt tgaccatatc
    16861 aacccctgat tctaatgctg taaccacttc attaaccgtc atacaacctg gtaaataagg
    16921 tatgctataa cgatgacaaa tcaaagccac tgctcgattg aaattaggac caacaataaa
    16981 ctgtgctcct gctaaaatag cttggcgggc agtgacatca tccaaaactg tcccagcacc
    17041 tatcaacacc tcaggatctt ctttgaagcg ttcagctaat tgaccgataa cctcactagc
    17101 aaaggggtta gtataggtga cttcaattgt cttaatgcca ccttcaatac tggccaggct
    17161 gcaagctaaa gcttcctcac tagactctcc tcgtacaacc aggacaagcc gattagcctt
    17221 aagtttggtt aagatttcta tttttcccat atcatcacct ctggacatct cgttgattag
    17281 ctaagactgc ttctacttgt tggacgctgt gatagagctg atcttcagct actgtacatt
    17341 ttaggctagc tgttgccaca gcaaaatcaa ggttgcgctg cttttcgtta ccttctaaca
    17401 actgataaag taaacccgcc acaaaagcat ctccacttcc aacccgttgc aagactgggg
    17461 tcgtcacttc acaggattcg taaaactgcc catcataggc ataagccttg aaacgattac
    17521 tgccttgggc atcatttgaa cgctccgtat ggtaaatagc ttgtaactga tagcgctgat
    17581 aaagtcctgc cagacgttct ctaatcatcg tagcatctgc cttattccta tcaaacatgt
    17641 caaaatcgtc actgtctaac agcaaaggtt ccatgccaaa aataatatcc gcgtattgag
    17701 caaagtgtga aaagagtcgt ttagcatcag ctaccgcaat catactagat ctaaagttaa
    17761 gatcaaaaga aacacaaata tcccttttct tagcttctct cacaagtacc tcaatcaggt
    17821 cctgagtttt tttgcccagt gccaaactga tcccactaaa atgaaagtga ctaatgcctt
    17881 caaaaatggc agctagatca agactatcct tatctaaggc cgaaaagcta gagccacagc
    17941 ggtcataata aacctgacta gaacgacaac caaagccatt ttcaaggtaa tacagcccaa
    18001 cacggttgcc ctgccaagcc gtcaaggtca tatccacacc gctttgcttc aaaaactgat
    18061 gaaaagcgtg accaacagga ttatctggta gggccgtaaa tagccttgct tctaagccaa
    18121 aaccgcctag agttcttgca atattaactt ctgaaccgcc gaaaaaaagc tgagcgtcgc
    18181 aggcattggt caaaggctga aactgattgg gactaacgcg tatcagcggt tcaccaacca
    18241 ataataactt agacatgtga tctcctttcc tcgtcaaaat ggaattactg ttcaagtact
    18301 gatttcaaat aagtaactaa ttctgacggc tccgcattgg caaagaagta ctctttaaaa
    18361 tgagcgccag aaatcgtttc tttgaggaaa tcttggtcga tgtctttgac aattgccaac
    18421 aaatctctgt aagtaatttt tttcagatca cttaaaatac gtgcattccg ctgttcagga
    18481 atagctcgcc ctttagggta accaccaccc attggttctg caaagaggcg ttcaaatatt
    18541 aaggtcaaat tcaactctgc tccccaacca aaacctttgg caaaaggaat ggacaaggca
    18601 ttaccaccat tgatttgaga aaagagataa gcttctgttg gctcactcgc aaaaccacag
    18661 gtaacacctg ggaagctgtt caaggccaac atagctccaa cacctgtccc acaaccagtc
    18721 accacaaaat cagccgcctt agtggtcaat aaaatagaag ctaagagtcc attttgaaca
    18781 taggtcaact ggctttcacc ttctgtgcca tacataccat agttaaagac ttggtagccg
    18841 tgtttatctg tcactgtggt aagggcatcg taaatgatac catttttagc cgcttggcta
    18901 ttttcattaa ttaatgcaat tttcatataa aatctctttc tagtgtgtga ttaaggttgt
    18961 tttccaatat aagctaaaat gccaccgtcg acataaagaa tatggccgtt gacaaaatta
    19021 ctggcatcgc tagctagaaa aacagctgga ccagctaagt cttctgttgt cccccaacgt
    19081 gctgctggtg ttttagcaat gataaattga tcaaaaggat gacgactacc atctgcttgt
    19141 cgctctctca acggagctgt ttggggcgtc gcaatgtagc caggaccaat cccattacat
    19201 tgaatgttgg cttctccaaa ctcagaggct atatttttgg ttaacatttt caagccacct
    19261 ttagccgccg cgtaggcact aactgtttca cgccccaatt cgctcatcat agaacagata
    19321 tttataattt taccatgtcc ctttgcaatc attgatggta atacagcttt tgacacaata
    19381 aaaggagcat ttaaatcaat atcaataact tgacgaaaat cttctgcggc catttcaagc
    19441 atcggcgtac gtctaataat acctgcattg ttgaccagaa tatcaataac acctacttca
    19501 tcttcaattt gactaaccat ctgttggata ccagcttcat cagtcacgtc acaaacatag
    19561 ccatgcgctt ctatccctaa ctctcgatag gcagccaacc ccttatcaac taactcttgt
    19621 ttgatgtcat taaacacaat agttgcaccc gcttgagcat aggctttggc aatttcaaaa
    19681 ccaataccgt aggatgctcc ggtaatcaag gcaatcttac cttgtaacga aaacatattt
    19741 tccataggat tctccttttt gttattaaat tgagtaaacc tgtttactaa gactataaca
    19801 aagtaaacta gttttgtcaa gattttatga aaacggttca aaaataaaaa agagaaacta
    19861 gctagctttt aaaaataggt tttattcctt aatggcattt cttttacata gtgttcttca
    19921 ttaaacaact atgattgatc ccttttgaca tgttatggag attaatctct attttctcta
    19981 aaaataaatc tcaaaaaagt attgacaaag aatcttgaaa gcgctacaat gtttttaagt
    20041 aaaccggtaa actaaaggag atttatttat gattgctatt atcgttatgg ggcatggaca
    20101 ttttgcgagt ggtattgttt cagccttaga attgatcgca ggcaaacaag aaaaggtcac
    20161 tgctattgat tttactgctg agatgaccgc cgctgatgtg caggaccaat tatctcgtgc
    20221 tttaatccca gaagaggaaa ccttagttct ttgtgacctt ctaggaggta ccccttttaa
    20281 agtcgctgca accttaatgg aatctcttcc aaacacgacg tgtaatgttc tttctggcct
    20341 taacttagcc atgctaattg aagctagttt tgcaagacaa acagcagctt cttttgacga
    20401 cttagtatta ggacttataa catgttctaa agaaggcatt gttgactgga aaactctttc
    20461 tcagcaagaa gacggtacga cagacgatga gttaggaggc atttaatggc acgatcttta
    20521 aaaaccattg ctcttgaacc tatcaagcag cctgaacgat ttaccaaaga agatttccta
    20581 agccaagaag acattactca ggcccttgac ttagccctca aacaagttcg gcttaatatg
    20641 gactatttta aagaagactt tccaacgcca gctaccaaag ataaccaata tgccattatg
    20701 gataatacag aatggaccaa tgccttttgg acaggttgcc tttggttagc ttacgaatac
    20761 agtggagatg atgccattaa agctctggct caagcaaatg acctttcttt cctagatcga
    20821 gtaacaagag acattgagct agatcaccat gaccttggct ttctttacac cccttcttgc
    20881 atggctgagt ggaaactttt aaaaacttct gaggctagag aagctgcttt gaaagcagct
    20941 gacaaattag tccaacgcta tcaagacaaa ggcggcttta ttcaagcttg gggggtgctg
    21001 ggtaaaaaag aagattaccg tttaatcatt gattgtctcc taaacatcca attactcttt
    21061 tttgctagcc aagaaactgg agacaaccgt taccgtgaca tggcgatcaa tcatttctac
    21121 gcctcagcca atcatgtcat ccgagatgac gcctctgctt accacacctt ctactttgat
    21181 ccagaaacag gcgatcctgt taaaggggtc acccgacaag gttatagcga tgattcggct
    21241 tgggccagag ggcaagcttg gggcatttat ggtattcctt tgacctatcg tttcctaaaa
    21301 gaacctgaac tcattcaact cttcaagggc atgacccatt atttcttgaa ccgtcttcct
    21361 aaagaccagg tgtcctactg ggatttaatt tttggagatg gtagcgagca gtctcgtgat
    21421 agttcagcaa cagccattgc tgtgtgtggt attcatgaaa tgttaaaaac cttaccagat
    21481 catgatcatg ataaaaaaac atatgaagct gccatgcaca gcatgctaag agccttgatt
    21541 aaggattatg ctaacaaaga tctcaatcct ggcgctcctc tgctacttca cggagtctat
    21601 tcttggcact ctggaaaagg ggttgacgag ggaaatatct ggggagacta ttactatctt
    21661 gaagctctgc ttcgtttcta caaagactgg aacccttact ggtaacaaac accgatacct
    21721 acatttaaaa aggagatttt atgacacaac caaacattat catgacgcga gttgatgagc
    21781 gcttgattca cggacagggg caattatggg tcaaatacct caactgcaac actgttatcg
    21841 tcgctaacga tgcggtttct gaagataaga ttcaacaatc cctcatgaaa acagttatcc
    21901 catccagtat tgccattcgt tttttctcca ttcaaaaagt gattgacatc attcataaag
    21961 caagtcctgc tcaaagtatc ttcatcgtgg tcaaagattt gcaagatgcc aaacacctag
    22021 ttgaaggggg tgttcccatc acagaaatca atatcggtaa tattcataaa actgatgata
    22081 aggttgcgat tacccagttt atttcgctgg gagaaacaga caagtctgct atttgttgct
    22141 tagcccatga ccaccaagtg gtttttaaca ccaaaacaac tccagctggc aatagcgcct
    22201 ctgacgttga catcttagat tatatttaat gaataaagga gattgatatg gatatcaatt
    22261 tgttacaggc acttttaatt ggtctttgga cagccttttg tttcagcgga atgttacttg
    22321 gcatctacac caaccgttgt attattctgt cttttggtgt cgggattatc ttaggtgact
    22381 tgccaactgc acttagcatg ggagctatct ccgaattagc ttatatggga tttggagtgg
    22441 gtgccggtgg taccgttcct cctaatccta tcggccctgg tatctttggt accttgatgg
    22501 ctattaccag tgctggtaaa gtcaccccag aagcggccct agccttatca acaccaatcg
    22561 cagttgccat ccaattcctt cagacattcg cctataccgc ttttgctggg gctcctgaaa
    22621 ccgctaaaaa acaattgcaa aaaggcaata ttagaggctt caaattcgct gctaatggga
    22681 ccatttggtc ttttgctatc cttggattag gacttggttt attaggtgcc ttgtcaatgg
    22741 atactctcct tcacttagtg gattatatcc cacctgtctt gctcaatgga ttgactgtcg
    22801 ctggtaaaat gttacctgct atcggatttg ccatgatctt atctgttatg gccaagaaag
    22861 aattgattcc ttttgtacta attggttatg tttgtgcagc ctacctccaa attccaacca
    22921 ttggtatcgc tattattggt atcattttcg ccttgaatga attttacaac aaacctaaac
    22981 aagtcgatgc aacaactgtc caaggaggcc aacaagatga ctggatctaa taaattaacc
    23041 aagaaagatt acctcaaaac agctctgaga gctttcttcc tacaaaatgg gtttaattac
    23101 agtaattacc aaggtatcgg atacgccaat gtcatctatc ctgccttgaa aaaacatttt
    23161 ggcagtgata aaaagggctt atatcaagct ttagaagata actgtgagtt ttacaatact
    23221 aaccctcact tccttccttt catcaccagt ctgcacttgg tcatgttaga aaatgatcgt
    23281 cctgaagaag aaacccgaaa catcaaaatg gcacttatgg ggcctttagc tggtatcggt
    23341 gactccttat cacaattttg cttggcgcca ctcttttcaa cgattgcagc ttccttagct
    23401 agtgatggtt tagttttagg tccaattttc ttcttcctag ccatgaatat tatcctaact
    23461 gctattaaaa tcggttctgg tctctatggc tataaagtcg gcacaagctt tattgataaa
    23521 ttaagtgaac aaatggcggt tgtgtcgcgc atggcaaata ttgtcggtgt taccgttatc
    23581 gctggattag ctgctacttc tgttaaaata actgttccca ttacttttgc ggctggcaag
    23641 gtggatgcgg ccaatacagc acaaaaattt gtgactattc aaggaatgct tgataaaatt
    23701 gctcctgcac ttttaccagc tctctttacc ttactcatgt attaccttat taaaaacaaa
    23761 aaatggacaa cctacaaatt ggttctcctc actgttctta tcggtattat cggtagctgg
    23821 ttaggtattt tagcttaatg gttcacagag acctgttatg agcataggga aatagttcct
    23881 aagcacatga tgaggtctct tttcttttgg aaaggaaatg tcctatgtct cttgccttca
    23941 cacgctttaa agaaacagtc aatccagact tttgcaggaa ttacctgcta gactatcagg
    24001 ctgacagcta tgctgatcga aaaaggatag ctgacctact cttaaccaat acctttttat
    24061 ttgaagataa ttgggatatg gagccctgcc acatccctta ccacttagat cccattactt
    24121 ggcaaaaggc tgtcacagat gatcctgagt ggaattttat gttaaatcga cagacctatc
    24181 tccaaacatt aatcctcgtc taccttgttg aaagagatga acggtatctc ctaaaagcca
    24241 aaagtttcat cctcaattgg attgaatcag ctattccttt agaccctaag gggttagcta
    24301 ctagaacctt agatactggc atccgttgtt ttgcttgggt caaatgtttg atttacctta
    24361 atctttttaa cgccttaacc aaacaagagg aaagcctcat cctagcttcc ctggaaaaac
    24421 aacttcaatt tctccatacc aactaccttg ataaatacag cctcagcaat tggggaatct
    24481 tgcaaaccac tgcgatttta ttagctgatg cctactttgg ttccgacctt gatattgctg
    24541 cagcaacagc tttcgctaga aaagaattga cgcaacaaat cgcactccaa attctcgaag
    24601 acggtagtca atttgaacaa tcaaccatgt atcatgtgga agtactcaaa gcccttctgg
    24661 aattaactgc tctagtccct gattatcttc cacagcttag acctactttg cttgccatga
    24721 gtgattatct cctcaaaatg actggtcctg accacaaaca gatccccttg ggagatagtg
    24781 attttaccga taccagagac attctaacac tagcagccac tatccttgaa gaaccacact
    24841 taaaagctgc tgcttttccc gcacttgata tcgatagtct tctccttcta ggagaaaaag
    24901 gtgtccacac ttttgaacag ttacccgtcc aaaccttacc aacttttgcg catcactttg
    24961 aacattccgg gcacattacc atcaatcaag aaaattatta tctttttttc aaaaatgggc
    25021 ccatcggtag ttcccatacc catagcgacc aaaatagtat ctgtctctat tataaaggac
    25081 aaccactttt ctgtgatgct gggcgataca cttacaaaga agaaccatta cgctatgccc
    25141 taaaaagcgc tagccatcat tctaccgcct tcttagaaga acaattacct gagcagatta
    25201 atagctcctg ggcttacctt tcttacccca aatccaatta ttgtcacctt cgacaaaatg
    25261 gacacgttta ctttattgaa ggtagctacc agacgcaatt ctctgatagg aacaactacc
    25321 aacatgaccg acaaattctc atcttaccac caggaatgtt tcttatcatt gatacgatac
    25381 aggctcaagg caaccactgt cttgtcagtc aatttattct tgataatcac ttagaagtaa
    25441 agacagatca ccttagtgac ctcagactaa tcagtgactg ccctttcact atcgaagaaa
    25501 cgattttgtc aaaaaaatac aaccaatacc tgactagtca caaactcatc aaacgaaaac
    25561 cctttaaaga caagggttgc accagtaccc tattagtccc tgacgatacc aaagttactc
    25621 ccttaacacc acttcaaaca ggcaaacgca acccaattga gaccgctctt gcttggcatt
    25681 taaaaggaaa gcagtttgac tacagtatct gcgttctcca agaggactta attaaaggag
    25741 aaaaactagt tctcctaaat ggtcataaaa ttagaggaaa ggtggttgtt atcaaccata
    25801 tcacgaatga gattattcga ttgaaacact aaatctttta cgagtcctta tgttataata
    25861 tgactaacca ttactaaatt aaaggagtgt atgcaatgca gcgcaaagta accatcaaag
    25921 atattgctga attggctaaa acttccaaaa cgacagtttc cttttacttg aacggtaaat
    25981 ttgacaagat gtcagaagaa actaaaaatc gcattaaaag cacgattgaa gctaccaact
    26041 ataaaccaag cattgctgcc agaagtctca atgcaaaatc aactaaactc attggcgtgg
    26101 ttattggtga tattaccaat agtttttcca atcaaattgt taaaggcatt gaaagcaaag
    26161 cccaggaatt tggttaccaa attatcatcg gaaacagcaa ctacgaccct tctcgcgaag
    26221 atgaactcat tgaaaagatg ctgaacctcg gagtggatgg ctttatcatt cagcctacct
    26281 ctaatttcag gaaatattct cgcattatcg atattaagaa gaaaaaagtg gtcttttttg
    26341 atagccaact ttacgaacat cgcacaaact gggtcaaaac taataattat gatgctgttt
    26401 atgataccat tcaacaatgt atcgataaag gttatgaaca ttttatcatg atcacgggta
    26461 accccaattt acttagcact cgtattgaac gcgcttcggg cttcattgat gtgttagaag
    26521 ctaatcatct tactcatcaa gaaatgatta ttgatgaaaa tcagacttct tcagaggcaa
    26581 ttgctcagtt tttgcaagga tcactgacta aaaagagttt ggtttttgtc cccaattgtt
    26641 gggctcttcc aaaagtattt acagctatga agtctcttaa atttaatata ccagagattg
    26701 gactagtagg atttgataac attgagtgga caaaattttc ttcaccaaca ctaacaacta
    26761 ttatccaacc cgcctatgaa gaaggcgaac aggctactaa gattttaatc gatgatattg
    26821 aaggtcacag tcaagaagcc aaacaacaaa tttttgactg tcaagtcaat tggcaagaat
    26881 caacttttta atgtcaggca taccaatagt ccttatcgtt gtaacgatta taaaaaaagg
    26941 tttgagacaa ttgtctcaaa ccttttagcg tctattaaat tgctttttca tcttgaccaa
    27001 gtgatcgttg aacagctttg acaagctcaa caagaacaac catcaagaga cttccgataa
    27061 taaccactaa ccattgtgtg atcgtcaagt gtgtcacatg gaagaattga ttaaatccag
    27121 gaacaacaac tgttgccatc aatgctacaa aggccactgg aatagaatag ttgaacaatt
    27181 tgtttttgaa caacccaaca gtaaagatgg attggtaaac agatttcacg ttgtaggcat
    27241 gtaccaactg aatcaaaccg agggtcacat aagccatggt caaagcatct gcatgaacat
    27301 catggtagct tgtgtgttct gggaacatca aggcaaaacc ataaacccca agaaccagca
    27361 tggtttggaa ggcaccttgg taaagaatgg cttccttcac cccaccatca aagaagcttg
    27421 acttacgtcc acgaggttta tgcttcatca caccaggctc agcaggttca acaccaagag
    27481 caatagctgg tagggtatcc gttaccaagt taatccaaag caagtgaact ggttgaagca
    27541 catcccaacc aaacaaggtt gcaaggaaga tggtaaagac ttctgccatg ttagcagaaa
    27601 gtaaatattg aattgttttt tgaatatttg agaagacctt acgtccttct tcaaccgcaa
    27661 cgataatggt tgcaaagtta tcatcggcaa gaaccatgtc agaagctccc ttagaaacct
    27721 ctgtacctgt aattcccata ccaataccaa tatctgccgt tttaagagat ggtgcatcgt
    27781 taaccccatc gccagtcatg gcaacaactt tgccttcatt ttgccaagcc ttaacgatac
    27841 gaactttatg ctcaggagat acacgggcat acacggagta ttgtttaaag actttttgga
    27901 actcttcatc tgataattcg ttgagttcag cacctgtaaa gacatgatca acgccatctt
    27961 cttcgataat accaagacgt ttcgcaatag cttttgctgt atcttggtgg tcaccagtaa
    28021 tcatgattgg acgaatgcca gcttccttag ctactttaac agcctgtgct gcttcaggac
    28081 gttcagggtc aatcatccct accaaaccag agaagacaag attagcctca acaatttctg
    28141 tttccagtgt tggaagagca tcagaatatt tgtaagccat catcaagaca cgaagagctt
    28201 gtttggcaag actcttattg gtatcaagaa tggttttttt atcagcatcc gtaataggac
    28261 gaatttgtcc attttcttca atctgtgtga cacgcttcaa caattggtct ggcgcacctt
    28321 taacagcgat gaaatatttg ccatcagctt gtttatgaat ggttgacatc aacttacggt
    28381 cagaatcaaa tggtaattct gccacacgag gttctgccac catagcctca cgtacgtcaa
    28441 aattgtggtc taacccaaac tgtaccaaag ctgtttctgt tgggtcacca attaacttcc
    28501 ctgaaggatc aactttagta tcattagcaa agttcattac acgaagtgtc gtattgtcaa
    28561 aagcaatatc agctgatgaa ctttggagtg tcccgttggt ataaactttt tcaacggtca
    28621 tttgattcat tgtcaaggta cccgttttat cagaagcaat gatttcagtt gatcctagcg
    28681 tttcaacagc tggtaattta cggataatgg cgttacgttt agcaagtact tgagttccaa
    28741 gagaaagcac aacagtaaca atcgcaggaa gtccctctgg aatagctgca acggcaagcg
    28801 ctactgaagt catcaagcct tcaagcgggt gttgaccacg caagaagact ccaacagcaa
    28861 aagtcaccgc tgcaatcaca aggatcgcat aagtcaaaat cttagaaagg ttgtcaaggt
    28921 tttgcttcaa tggtgtatct gtctcatcag cattagcaag cataccagca atatgcccaa
    28981 cctcggtgta catgccagtg ttagtgataa ccccaatccc acgaccatat gtcacatttg
    29041 agttttgata tcccatattg acgcgatcac caataccggc atcttctgaa acagccgttg
    29101 acaagtcttt ttcaaccggt acagattctc cagtaagcgc tgcttcttcg atttttaagg
    29161 agttagcttc caaaagacgc agatcagcag ggacaacatc tcccgcttct agcaagacaa
    29221 tatctccagg gaccaattct ttagagtcta tctcagtcac atgcccatca cggcgaatac
    29281 gagccagtgg acttgacatt gatttcaagg cttcaatagc agcttcagct tgtccttctt
    29341 ggtaaacacc aaaagcagca tttaggataa caacagccaa gataataatg gcgtctgtca
    29401 aaccttccat cccctctgta agaactgaaa gaagggctgc cacaatcaaa ataataatca
    29461 tcaaatcctt aaattgatca aggaatttca taaataaact tcgtttttcg ccttcatcaa
    29521 gctcattacg accatactct gctaagcgct cttttgcttg ggcagacgta agaccttcgc
    29581 gactggtctc taattgagcc agtacggtct cttcactttg cgtgtaaaac gcttcatgtc
    29641 tttgttcttt agacaaatta gtacctcctt ggtcttttta ttttagttat tcaaataaaa
    29701 agacctgttt attttcttaa acaagtctcg ctgtttaaga ttgtaccgga aaccgaaatt
    29761 ccgtattgac gatacaatca cggctagccg tctgctactc ccttgatata ataaccatta
    29821 tagtaaaaac ctgactaaaa aacaagaata atccctaatc agatgattta ctgctatctt
    29881 tttagttaat cccaactcag ctcaagatga tagcttgcaa atagcatttc tgtttgagct
    29941 tgaagcaaat gataatgaag gtccaatttt tgacaagaac aatctaagtg gtaatgactg
    30001 gtatctgtca aaaattgttg aacccccatc ggcgtaggta gagcaatcag gaccttcttg
    30061 cccgcaaaaa atttcatgat tgactgaggg ttggaaaaac ggctcatggt cagttctgtc
    30121 tcattgtact tgatgacaac cttttccttg tcagtgtttt gatagatgag gtactgatag
    30181 cccccttttt ctcgccactc acaatcatga atttcttgaa taatctctgt ctcatctcca
    30241 aagcgaatat ggttggttaa ctgtaatttc ataactcttc tttctattct aagctcgtct
    30301 gtttattcta tcaatttctc cttattttat caatttttga tataataaaa attatgaatg
    30361 aaaaagtatt tcgtgaccct gttcacaact atatccacat tgataatcca ttaatttatg
    30421 accttatcaa taccaaagaa ttccaacgct tacgacgcat caaacaagtc cctacaacag
    30481 ctttcacctt ccacggcgca gaacatagcc gtttttctca ctgcctaggt gtttacgaaa
    30541 ttgcccgtcg ggtgacagct atttttgaag agaaatacgc tgatatctgg aataaagatg
    30601 aaagtctggt cactatgacg gctgccctct tacatgacat tggtcatgga gcctattctc
    30661 atacctttga ggctcttttt catacggatc acgaggcctt tactcaggag ataattacca
    30721 atcctgagac agaaatcaat gccattttgg tgcgtcatgc tcctgatttt ccagacaaag
    30781 ttgccagcgt gattaatcat acttatccta acaagcaagt tgtgcaactg atttccagcc
    30841 agattgactg cgaccgtatg gattaccttt taagagattc ttatttttct gctgccaatt
    30901 acggtcagtt tgatttaatg cgtatcctac gtgtgattcg acctgttgaa gatggcattg
    30961 tctttgaaca tagtggtatg catgctgtcg aggactatat tgtcagtcgt tttcaaatgt
    31021 acatgcaggt ctattttcat cccgccagcc gtgctgttga gctcatctta caaaatctcc
    31081 tcaaaagagc ccaacactta tatcctgagc aacaggctta tttccaaaaa acagctcctg
    31141 gactcatccc cttctttgaa aagaaggcta atttagcaga ttatatagcc ctagatgacg
    31201 gagtcatgaa tacttacttc caagtctgga tggcaagcga agaccatatc ttatcagatt
    31261 tggccagtcg ctttattaat cgaaaaattc tgaagtctgt tacctttgac caagattccc
    31321 gaggagagtt agaacgcttg cgccaattgg tcgaatcagt tggctttgat ccagattatt
    31381 acaccggtat ccatatcaac ttcgacctgc cttatgatat ttatcgacca gagctcgaaa
    31441 atccaagaac tcagattgaa atgatgcaaa aagacgggag ccttgctgaa ttatctcagt
    31501 tatctcctat tgtcaaagca ttaacaggga caacctacgg agaccgtcgc ttttattttc
    31561 caaaggaaat gttagagctc gatgacctct ttgctcctag caaggaaact tttatgagtt
    31621 atatttccaa tgggcatttt cactttagtc agtaaagccc tttaagattg cagaaaaagt
    31681 cttttttgat acttttctta ggtggcactt atggcagcga gttcctttgg aattctatgc
    31741 tttatttttg agcctagggt ctcaaaaatc ccgcgtaacg tcactgccag tattgacacc
    31801 acttttgcca cagcaaaaag cagctgtttt ggctcacgtc acacgcagaa ataagaaatc
    31861 agttgtcaaa tcagcagagc agttcatgct ttttgagcat caaactgggt ttgtctccat
    31921 tctaagattg aagaaagagt actttcttca atctttttag ttactacaga ggcgattgag
    31981 ctaaactaaa acgttttctc tcgtttttcc ttaagtttcc tttcccatcc catgacaaaa
    32041 gcacctattt ttagtaagat aggagggaga acatttttaa gataaaccac aaaaaaacaa
    32101 ggaggatgcc atgtctatca aactggttgc tgtcgatatt gatggcacat tactcactga
    32161 tgaccgtcga attacagatg atgtttttca agctgtccaa gaggctaaag cccaaggtgt
    32221 tcatgtggtg attgctactg gcagacctat tgccggagtg atttcattac tcgaacaatt
    32281 agaactcaac cacaaaggaa accacgttat cacttttaat ggtggcttgg tgcaagatgc
    32341 cgagaccggc gaagaaattg ttaaagaatt gatgacttac gacgattatc tggaaacgga
    32401 atttttgagc cgaaaattag gagttcacat gcatgccatt accaaagaag gtatttacac
    32461 tgccaatcgg aatattggaa aatacacagt tcatgaatcg actttggtaa acatgcctat
    32521 tttctatcgc actcccgaag aaatgaccaa taaagaaatc atcaaaatga tgatgattga
    32581 tgaacctgac cttttggacg cggccatcaa gcaaattcct caacatttct ttgataagta
    32641 caccatcgtc aagtcaaccc ctttttattt agagttcatg ccaaaaaccg tcagcaaggg
    32701 taatgccatc aaacacttag ctaaaaaact cgggctagat atgtcacaaa ccatggccat
    32761 cggggatgct gaaaatgatc gtgctatgtt agaagtcgtt gccaaccctg ttgtcatgga
    32821 aaatggggtt cctgaattga aaaaaatagc caaatacatc accaaatcca acaatgacag
    32881 tggtgtggca catgctattc gcaagtgggt attaaactaa tgtatacata taaaatcgga
    32941 atatctcctg aagaacatga tcagtttgtc ttagcacagc cacaagctgg cttacttcaa
    33001 agttcaaagt ggggaaaagt caaagataac tggaaacatg aacgcatcag cttttatgaa
    33061 aatggggtac aagttgctgc tgctgcttgt ttaatcagaa aacttcctct tggtttcact
    33121 atgatttata tcccacgtgg gccaatcatg gactacgcca attttgaact ccttgatttc
    33181 gttattaaaa cgctcaaaac tttcggaaaa agcaaacgag cgctttttgt caaaattgat
    33241 ccaagtttag tcatcaaaca aactttagaa ggaaaagaaa gcaaagaaaa tgatgtgaca
    33301 ctctctatta ttgcttttct aaaaaaactt ggtgtcgaat ggtctggcag aacaaaagaa
    33361 ctcgaagaca ctattcaacc aagaatacaa gccaacatct acgccaagga ttttgacttt
    33421 gacagcctgc ccaaaaaagc caagcaatcc attcgtaccg ctaccaataa aggtgttaat
    33481 gtcacgattg gtggcagtga attgctagac gacttttcag ccttgatgaa aaaaaccgag
    33541 aatcgaaaag gcattatcct tcgaggcaaa tcctattatc aaaaattact aggtatttat
    33601 gctggtcagt cctacattac tatggctagt ttagacttac ctgaacaaaa aaaactcttg
    33661 atccaacagt tggacaaggc cttagcagaa caagcacgat taacagataa gagtaaacct
    33721 agcaaagtag cagaaaatca aaaaaccatt gcacggctac aaaaagattt aaccatttta
    33781 agcgagcagc tcgctgcagg caaaacccgc atcccactag ccgccaccct aaccttgatt
    33841 tatggcgaga cttctgaaaa tctttatgcg ggaatggacg atgattatcg caattatcaa
    33901 gctcctcttt taacctggta tgaaacggct aaagaagctt ttaaaagagg ctgccgctgg
    33961 cataatttag gaggtgtgga aaatcaacaa gatggtggac tctatcattt caaagctaga
    34021 ctcaacccca ccatcgaaga atttgcaggt gaattcaaca ttcctgtcgg ccttgtttca
    34081 agcttagcca tcttgaccta taacttaaga aaaaaattaa ggagcacaac taatggcact
    34141 aattgaaatt tcacaagaac aatttgacca ttactgccac agtctcgtcc atcattcctt
    34201 tatccaaacc tcagaaatgg ccagcttgat ggctaaaaga ggggcaaaac cacaatttct
    34261 tggacttgaa aaagatggtg aactaaaggt tgctgctatg gtctttagcc aaaaagtggc
    34321 tggaggttgg cgtatggaac tcaatgcagg ccctaatacc aatcatcccg aagaattaga
    34381 acatttttac actcaactta aagactatgc caaacaaaaa gacgttatcg aactcatttt
    34441 aaaaccttac gacaattatc agtcatttga tactgatggc atccctattt ctcatccaaa
    34501 cactgacctt attagccttt taacggcgtt aggatataaa catgacggtt taaaaacagg
    34561 ctatcctgag ggtgaacctg tttggcatta cgttaaaaag ttagaaggaa ttgattcctc
    34621 acgtttaacc cattccttca gcaaaaaagg aaaagcttta ataaaaaaag ctaatacttt
    34681 tggtattaaa ttacgtcaac tgaaacgaga tgagttacac catttcaaag aaatcactga
    34741 agcaacctct gatcgtagag attaccttga caaatcatta tcttactatc aggactttta
    34801 tgatagtttt ggagactctt gtgaatttat ggttgcgact cttaattttg aagattacct
    34861 taataacctt aaacaaagac aactacaact agcaaccagc atcaacaaag ttaaaggaga
    34921 ccttggcaaa aatcctcatt ctgaaaaaaa gcaaaataga ttaaaagaac tatccagcca
    34981 attcgaaacc tttcaggtaa gaatttctga agctctccat tttcttgaag agtatggaac
    35041 taaagatgtg ttccttgcgg gaagtctttt tatctatacc gaacaagagg ccgtctacct
    35101 cttttcagga tcttacccca aatttaataa attttatagc ccagctctat tacaagagca
    35161 tgctatgctc aaggcaattc ataagggcat caaacaatat aattttcttg gtattactgg
    35221 aaagtttgat ggttcagacg gtgtcttgcg ctttaaacag aatttcaatg gctttatttt
    35281 gcaaaaacca gggacattta gatgttatcc atttccaatc aaatatcatt tcattagatt
    35341 ggcaaaaaag ctattaaacc gctattaagc taatgcattt acctgtagaa tcacctttat
    35401 tctctcatag taagagttaa gtcatctaaa aaagaaaagg atttaacctt ttctttttta
    35461 gatgacttag ttaaggaaat caagcaaagc aaggaagcta tcagcttcaa gtgatgcgcc
    35521 acctacaagg gcaccgtcaa catctggaca agccatgtaa tctttaacgt tttcaggttt
    35581 tacagaacca ccgtattgaa cgcgaacttt atcagcaact tcttgcccaa agtctgctgc
    35641 taccacgtca cgaacagctt tacacatgtt ttgagcatca tcttgagtag ctgatttacc
    35701 agtaccgata gcccagattg gctcataagc taaaacaagt gaagctactt gctctgctga
    35761 aagacctgca agtgctgctg aaacttgcgc acctacaaac tcaacggctt taccagcttc
    35821 ataagtttca agagactcac cacaacaaac aattggagtt aaaccgttag caaagattgc
    35881 tttggctttt ttattaatgt cttcatctgt ttcatggaag taatcacggc gttctgagtg
    35941 accaatcaca acatagtcag ctcccatttc agcaagcact tttggtgatg tttcaccagt
    36001 gaatgcccct gtgttttcaa agtagcagtt ttgtgcagca actttaagaa ctgaatcttt
    36061 agcagcttca atagtggtta ccaaatcaac agctggggct gctacggcaa catcaacaag
    36121 gtcagttgaa ggtaatttgc ttgctacagc ctcaacgaat gcttttgctt cttgagggtt
    36181 tttgttcatt ttccagttac cagcaataat tggtttacgt gacatatcac atacctcttc
    36241 tttttttatt tatatcttct attttagcac aaatgaccga cgattaaaag gaattgctta
    36301 tattttagga atattttcac aaataaaaac tctttttctg aaagcttttc attcttattt
    36361 atcagcattt ta
//
LOCUS       DAUGFP010000015        32724 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.015, whole genome shotgun sequence.
ACCESSION   DAUGFP010000015 DAUGFP010000000
VERSION     DAUGFP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32724
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.015"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            9..170
                     /gene="sagA"
                     /locus_tag="VH663_000753"
     CDS             9..170
                     /gene="sagA"
                     /locus_tag="VH663_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEQ8173971.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            392..1342
                     /gene="sagB"
                     /locus_tag="VH663_000754"
     CDS             392..1342
                     /gene="sagB"
                     /locus_tag="VH663_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEQ8173972.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1339..2397
                     /locus_tag="VH663_000755"
     CDS             1339..2397
                     /locus_tag="VH663_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEQ8173973.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAINTETYENTLEPETFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNDPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSDALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHACTLNPPHSADFDSLERRVLARLQDSTLYQHFANQILPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2417..3775
                     /locus_tag="VH663_000756"
     CDS             2417..3775
                     /locus_tag="VH663_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEQ8173974.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3750..4421
                     /locus_tag="VH663_000757"
     CDS             3750..4421
                     /locus_tag="VH663_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ8173975.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLIELIFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKEVT"
     gene            4418..5101
                     /locus_tag="VH663_000758"
     CDS             4418..5101
                     /locus_tag="VH663_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEQ8173976.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLMALSYQDFLDV
                     LLSLFQFVVIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIVILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5124..6047
                     /locus_tag="VH663_000759"
     CDS             5124..6047
                     /locus_tag="VH663_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8173977.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6056..7183
                     /locus_tag="VH663_000760"
     CDS             6056..7183
                     /locus_tag="VH663_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173978.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIRLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            7180..8298
                     /locus_tag="VH663_000761"
     CDS             7180..8298
                     /locus_tag="VH663_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8173979.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSN
                     LSRFRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGGTLSQAWPYLLALMGTALALIIFSSLLLKPTRL"
     gene            8870..11602
                     /locus_tag="VH663_000762"
     CDS             8870..11602
                     /locus_tag="VH663_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEQ8173980.1"
                     /translation="MINKKYIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTSLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVIKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGCVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      11767..11882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            11880..12380
                     /locus_tag="VH663_000763"
     CDS             11880..12380
                     /locus_tag="VH663_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEQ8173981.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            12574..14532
                     /gene="ligA"
                     /locus_tag="VH663_000764"
     CDS             12574..14532
                     /gene="ligA"
                     /locus_tag="VH663_000764"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEQ8173982.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWNFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKEDFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRKL"
     gene            14546..15568
                     /locus_tag="VH663_000765"
     CDS             14546..15568
                     /locus_tag="VH663_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ8173983.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            15960..16157
                     /locus_tag="VH663_000766"
     CDS             15960..16157
                     /locus_tag="VH663_000766"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEQ8173984.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            16192..16908
                     /gene="atpB"
                     /locus_tag="VH663_000767"
     CDS             16192..16908
                     /gene="atpB"
                     /locus_tag="VH663_000767"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEQ8173985.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            16926..17420
                     /gene="atpF"
                     /locus_tag="VH663_000768"
     CDS             16926..17420
                     /gene="atpF"
                     /locus_tag="VH663_000768"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEQ8173986.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            17420..17956
                     /locus_tag="VH663_000769"
     CDS             17420..17956
                     /locus_tag="VH663_000769"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEQ8173987.1"
                     /translation="MTKKEQAIIEQYAKSLVEVASEHHSLDTLQADVLAILETFETTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQAVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKNRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            17972..19480
                     /locus_tag="VH663_000770"
     CDS             17972..19480
                     /locus_tag="VH663_000770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEQ8173988.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            19496..20371
                     /locus_tag="VH663_000771"
     CDS             19496..20371
                     /locus_tag="VH663_000771"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEQ8173989.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            20533..21939
                     /gene="atpD"
                     /locus_tag="VH663_000772"
     CDS             20533..21939
                     /gene="atpD"
                     /locus_tag="VH663_000772"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEQ8173990.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDHLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            21952..22368
                     /locus_tag="VH663_000773"
     CDS             21952..22368
                     /locus_tag="VH663_000773"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEQ8173991.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            22634..22891
                     /locus_tag="VH663_000774"
     CDS             22634..22891
                     /locus_tag="VH663_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEQ8173992.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            22956..24227
                     /gene="murA"
                     /locus_tag="VH663_000775"
     CDS             22956..24227
                     /gene="murA"
                     /locus_tag="VH663_000775"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ8173993.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTHLRGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            24231..24419
                     /locus_tag="VH663_000776"
     CDS             24231..24419
                     /locus_tag="VH663_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ8173994.1"
                     /translation="MTNGLKYVLEQMLLLFIIAALACLFLAIGLMIGYSFMGDGQSPW
                     HILSMDKWAELVNKFTGK"
     gene            24455..24994
                     /locus_tag="VH663_000777"
     CDS             24455..24994
                     /locus_tag="VH663_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEQ8173995.1"
                     /translation="MLTRKKRKRVQEQKVPKRANALLTKSTRQYRNRYQTDNAYRTFK
                     PAGWHRLHHLKGSYDHAVDRGHLLGYALVGGLKGFDASTGNPDNIATQLSWANQANKP
                     YSTGQNYYEGLVCRALDKGHRVRYRVTLLYDGDNLLASGSHLEAKSSDDSLTFNVFVP
                     NVQAGLTVDYRTGQIAINL"
     gene            25277..26320
                     /gene="pheS"
                     /locus_tag="VH663_000778"
     CDS             25277..26320
                     /gene="pheS"
                     /locus_tag="VH663_000778"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEQ8173996.1"
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLEMIIKKMFGDERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            26530..28935
                     /locus_tag="VH663_000779"
     CDS             26530..28935
                     /locus_tag="VH663_000779"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEQ8173997.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPADGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDN
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDANS
                     QTVVLEVAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEVGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKENLPNEVNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLASMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELNMAALEAALSSDQTFVEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTKIKLFDVYEGVTIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLTEQVGA
                     EVR"
     gene            29046..29423
                     /locus_tag="VH663_000780"
     CDS             29046..29423
                     /locus_tag="VH663_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEQ8173998.1"
                     /translation="MYQTKITGNRLYRTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVTVTITLEGLSHV"
     gene            29416..29802
                     /locus_tag="VH663_000781"
     CDS             29416..29802
                     /locus_tag="VH663_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEQ8173999.1"
                     /translation="MSDASLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            29875..30951
                     /locus_tag="VH663_000782"
     CDS             29875..30951
                     /locus_tag="VH663_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174000.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSVLLAKDADATLTLSQVSRAQENQITADKVAPLAQLNTVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GIGNITIAQAVLLQTFLLSALGSGLGLLGTWLTSLVLPEAVPFQSNWLVYLAILVSMV
                     CFALLGTLFSVFSIVRIDPLKAIG"
     gene            30961..31629
                     /locus_tag="VH663_000783"
     CDS             30961..31629
                     /locus_tag="VH663_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174001.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGITGLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLKYCDKVYQMQDGEL
                     RQER"
     gene            complement(31733..32650)
                     /locus_tag="VH663_000784"
     CDS             complement(31733..32650)
                     /locus_tag="VH663_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEQ8174002.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSGVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRAVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
BASE COUNT        10061 a         5555 c         6842 g        10266 t
ORIGIN      
        1 taaaccttat gttaaaattt acttcaaata ttttagctac tagtgtagct gaaacaactc
       61 aagttgctcc tggaggctgc tgttgctgct gtactacttg ttgcttctca attgctactg
      121 gaagtggtaa ttctcaaggt ggtagcggaa gttatacgcc aggtaaataa tctatttagc
      181 atctctatgt gatagtgata ttaaggtaat gagtttgcta gcaactaagt tatctttggt
      241 aacttctgat tatagatgaa tggcatagag gtgttagaaa acatgagaca aaagtaatct
      301 tcttgatgct aacgtctcag gtaattagca ggtactagat agtacctgct aattactata
      361 tgtttagtaa aatgagatag gaaagatagt tatgtcattt tttacaaagg aacaacaacc
      421 taaagagaat tgtccgccaa taactgttga aaaagcaagg caattgtttg aatttaatac
      481 aaaccacttg tccttatcgg attaccatca tcaaacggtg ctaaaaacgt caaagcagct
      541 agttgctcaa catttaatgc ctaatgcaac cgataatctt agtcaacatt ttttgatgaa
      601 ctataaagct aataataatt atttaggctt ccaagctagt attgtcgact tttttacaga
      661 ttctgccgtt gctaattttt caagtagtta cgtttatgaa agtcaggaaa agataattcg
      721 tttaccaaaa cctaccaaga tatcaactgc tctgtcgaca tgtattataa aacgaagaag
      781 tcatcgtcaa ttttcagata gacaaatgcc tcttcaagat ttatcaaaca ttctttatta
      841 tgcatgtggt gttagttcac aagcatcaat tagagatgga gcatcagata agattacact
      901 cagaaactgt gcttcaggtg gaggtttata ccctattcat ttagtttttt atgctagaaa
      961 catcagtaaa ttaatagatg gtttctatga atacctaccc tatcagcatg cactaaggtg
     1021 ttatcggcat agctctgagg aaaacgttag agattttgcg gaatacggtg ctattaatgc
     1081 tgaaaattgt aatattatta ttatttatgt ctaccattac atgaaaaata cacgtaaata
     1141 tgggaatcag gcgactgcct atgcttttat tgaatcagga gaaatagccc agaatattca
     1201 attgactgca actgccttaa cttatggaag tattgatatt ggtggttata ataaggaata
     1261 tctccaagaa ttattagatt tagatgggct aggagagcat gtgattcaca tgacactcgt
     1321 aggaactaag gagtctcaat gaaatatcaa cttaatagta atgttcgtgt cgtgacattt
     1381 caagatacat tttgttttag aaaaggaatc tgggacttta atgaagccat tctagatttg
     1441 acacaagaac ctcaaaattt aaagatagtt tatcaagaaa tagtttcaca gttggttaag
     1501 ggagtagcca ttaatactga aacttatgaa aatactttag aacctgagac gttcgctaat
     1561 ttaatggaag tcatttcagg cttgtattac aatgatatgc tgatgcttga ggatgactac
     1621 aatcttgaag aaaatgtcat gaaaatttta atgggtaatt tccgttttat ggcgcaagca
     1681 ggtcatatgg taagcaatga tcctgttttg tttatcagtg attcgactta tgttaatgaa
     1741 tctgctgagt tgctagctga acaactgcat ttacaattgc aagtggctag cgatgactta
     1801 aaaatgttaa ttcagcagac agatgttagt tcgcgtttag atgctttgga gcatcgccgt
     1861 aacatgaatt gtttatcaga tgccttgtgc gattaccaaa gtattattat ttgtcaagag
     1921 cgcttgaata ttatgatgtt gcgacacttg aatgaagtta gcgtagctat gaaaaaacaa
     1981 ttagtgattg gttttgtaga tggaccattc ttgcacgctt gtactttgaa tcctcctcat
     2041 agtgctgact ttgatagctt agaaagaaga gttttggcac gtttacaaga ctcaactctc
     2101 tatcaacatt ttgctaatca gattttacca gcaacacaag atgtgagcca ggcctattta
     2161 ccattgttaa atgttttaat gaatttagtt gttagcgaag ctttcattat tgctcaaaca
     2221 ggtagttcta aatttgaggg acgtttatta agcatttact taccgacgct tgagattcag
     2281 gtccaagata ttttgaaaat gtctaattca cagacacaag gagctttggc aaaattaaag
     2341 tatgaggatc agcagatatc aacacgtgag attgttaaaa aactccttga cgagtaacct
     2401 ataaggagaa tctattatgt tatactatta tccttctttt aatcatattt ttgatgaatt
     2461 aaaaagttta agtggcaatc gaacaggtat cttaaatcaa tctcaagttc cagtttgcaa
     2521 ccatcctcac gatgtttact taaaaagtat tactggccaa atgccagatt atcataaaca
     2581 atttataggt gagttgagtc aagttagtta tcacattatt ggttatggca gttactatga
     2641 agaagcactt atcaagtatt taggagaaag tattgaacgc tatgctactg taattgccgg
     2701 agacttacta tcagatcgta ttgtctatgc ttcttataat gagttaaaac tgttacataa
     2761 agtgatgcct ttagaatatt tgcaagtgtt tacccaagaa cagatcgcat tatcttgtga
     2821 cttgcaaatg atgatgtgcg ataaaatggt aactgagaac gatgtattgg gctgggttaa
     2881 atgtcccatg ttctttgagg acgcagagat gtatgttcca gctcaaatgc tttgtgttgg
     2941 gtataaaaca aatgagactg taggagaaag acggataata ccaggatttt ctacgggaac
     3001 agcttctcac aaaacgttag aagcggctat gtgcaacagt ttgattgaat acatccagat
     3061 cgattcaatg atgcttagct ggtatactaa gaagccttgc cccaagatta ttgttgatga
     3121 tcctgatatt gaagtgattt tagaagaagc tagactggga aaagacagtc tatacgacat
     3181 tattcctatt gatatgactg taggagaaga taatcctctt tatacgtttg gaatcattct
     3241 caaaaataaa tatgatgaag ggccttacct cttgtttgga gtgcaggctg ggttagatcc
     3301 taaacatgct ttgctaagag ggatcatgga agcttcagct attagctata gttactacta
     3361 taatctttta tatcaaaaag cttcccttgc aaatattgaa tgcgaagaac ctttgttttt
     3421 agatttagat agtaatgtct tctattatgc acatcccaag gaccaagacc ataaatggaa
     3481 ggcctttgaa cctttgattt caggagaagt tctgttaagt gatttggaag atcattctgg
     3541 taaagataaa aaagaagatt tgaagacttt attagcttat gctaagaaag tcagtcctaa
     3601 tgctgtcttt ttagatatta cacctcctga agctctagaa aaaggctggt acgtcacgcg
     3661 agttctgatg ccagaacttc ttgaaatgtg tataccagca tttccttttg ctaatcatcc
     3721 acgaatgaga cagtttggag gtgtaacaaa tgcctttgtc catccaatgc cttaatcttt
     3781 gttttttgtt ggtaactttt tgtccttcaa ttcctatgca agctattttt ggaaaggaag
     3841 atagtggtta tgcatttaat ttaattggtt ttctaagagc aactctgatt tatgacattt
     3901 tggctttggt aagtatttat gttttgtcac ctcaaataac cttgtctctt gaaagtattg
     3961 atagtaagac gttctttatg ggacttgtat tttgcgtctt gatagtactg attgaactca
     4021 ttttcttaca tggtttacgt tgttggcaaa aaaagcaatg gcttcctgca actttctcgt
     4081 ttgtaggaac gacaaatgat tggtctaaga ttggctatcc tttattacta gctttatttg
     4141 aagagacgat ttatcgtttt ttgtggttta atatattagc ttttcaatgg catttaccaa
     4201 caattattgt tttaattgta actagttttt gctatgcttt aaatcactta ttgatgggga
     4261 aatccatttt ttatgctaag ttggtaacag gcatcattta tggtagtatt tacatgctga
     4321 ctagtcaatt gtggttggtt gtgataatgc atgtaggagg taacttgcta gttgagtgtc
     4381 ttagccatct tcaaacaaaa aagaagaagg aggtgacatg atgctattgg ttttgctgtc
     4441 gtttttaggt ctaactactc tttggttgac cagttatcga agacgttgtt tggctagatg
     4501 gcatcttcaa tggttaatgg ccttatccta tcaagatttt ttagatgtct tactgagtct
     4561 gtttcaattt gtcgttatta tacttgtttt gttcttttac tcagctacta ttaatctagg
     4621 agaggttctg acctttttga cacaaactag ctggcattgg cagattcttt gttatttagt
     4681 gttgtatctt atggccatca tagagatgac gcttttagtt ttaattctta tctttgatgt
     4741 tttgctccag aaagatagtc gattgctctt taaaaaaata acttggctgc cttttagaca
     4801 agataaacca gtcttaagta tacttttgct aggcatagtt attctagtag acagtctctt
     4861 ttatttgggc ttgcttatat tattaggaga acagtcgcta acaagtttag caacgcttat
     4921 tttaggttat gggatagtca aggcttgtcg ctattcaggt tggttaaatg tactattagc
     4981 tttttgtctt ttcactatag ttggactatg ggcagttacg gcaactttat tatatggttg
     5041 gctagtaggt gcgatgatat taacggtgac ttatttgatg attagttgca aagagtatta
     5101 gcaatattaa aaaggagtga tatatgagtt ttgtacaatt aacaaatgtt gtcaagtcct
     5161 acaaaaacgg caagaaagct gtcaatgatg tttccttgtc tattgaagca ggtaatattt
     5221 atggtttgtt aggaccaaat ggtgctggta agtctacctt gattaatctt atcttaggct
     5281 tgatcccttt gagttccggt aaaattactg ttttagggca atcccaaaag actattcgaa
     5341 aaataagttc gcagataggt tatgttcctc aagacattgc tgtttatcca gacctaactg
     5401 cttatgaaaa tgtagaacta tttgggtcac tttatggctt aaagggagct cagcttaaaa
     5461 aacaagttct aaaaagttta gaatttgtgg gactacactc ccaagctaag cagtttccaa
     5521 gtcaattctc aggaggaatg aagagacggt taaatatcgc ttgtgcgcta gttcattcac
     5581 ctaaattaat catttttgac gaaccgactg tagggattga tcctcaatca cgtaatcata
     5641 ttttagagtc gattcgtttg ctaaacaaag aaggtgccac agttatttat acgacccatt
     5701 atatggaaga agtagaggct ctttgtgatt atatttttat tatggatcat ggtcaagtta
     5761 ttgaagaagg acctaaattt gaactggaaa aacgttacgt tgcaaatcta gcaaaccaga
     5821 ttattgtaac tctaacagat tcacgtcatt tggaactggc agataagcct gattggtctt
     5881 tgatagaaga tggagaaaaa ctcatgttga agattgataa tagtgatatg acatcagttg
     5941 ttcatcagct cacacaggcc aatattactt ttagcgagat tagacataac catttgaatt
     6001 tagaagaaat tttcttacac ttaacaggta agaagttacg agattagagg ttactatggt
     6061 tttatttcat ttaatcaaaa aagaaagttt acagattttt agaaaccgaa cagccttatt
     6121 gatgatggtg atttttccaa ttttgatgat cgttatttta agttttgcct ttaaatcaag
     6181 ttttaatact gcgacaacag tccctaaatt gaccattcgt taccaattag agggtgaaaa
     6241 aacggattac cagaaaaatt ttcttgcttt tttaaaagtt ttaaaccaaa aacttcattt
     6301 agaggctaaa cctagtaatt ctcttgaaaa agatcgacaa agggtcagtg aaggagcctt
     6361 aacggctgtt ttagaagtga agaagaatca gaccattaag gttattacta ataatattaa
     6421 tcagcaaaat gcagatttaa tcaatatgct agtaaaaaat tatgtagata atgctaaaac
     6481 ttatgactcg atagcagctc tttatcctca acaattaaat catatcagaa agcgaagtgt
     6541 ggactatgtt aaggtcagtt caatacagac aagtaaagga atgacatcag ctgattatta
     6601 tgctatttcc atgtttacca tgattacttt ttatagtatg atgtctgcga tgaaccttgt
     6661 tttgtcagat cgtcaacaac ggattacaaa tcgtattcgc ttaacaggag tttctccaag
     6721 tcttttagtc tttgggaaat taataggtgc tatgttagca acaactgttc aattgagtct
     6781 tttatacatt tttacaaggt ttgttttacg agttaattgg ggcactaatg agtggatgct
     6841 tattggtata acagcctcct tagtctatct ctctgtagct ataggtatcg ggctaggcat
     6901 aagcattaaa aatgaggctt ttttaacggt tgcatccaat actattattc ctatattcgc
     6961 ctttttagga ggcagttacg ttccgttgac aacattacac agctctatta ttaatcaatt
     7021 gtcgaatata tctcctatta aatgggttaa tgatagtttg ttctatctta tttttggtgg
     7081 tcaatataat ccgattcctg taactttgat cgttaatatt agtatcggaa caatttttat
     7141 aatattggca ttaataggta tgagaaagca ggtgacgaca tgatttgttt tataaaaact
     7201 ctatttgtta agataaagcg caaaaaaaca agttatgtga cattcttttt aatgcctatt
     7261 ttaacgacac ttttggcttt atctttgagt ttttcaaata ataatcaggc taaaattggg
     7321 atattagata aagataatag tcagatatct aaacaattta ttgctcaact aaagcaaaac
     7381 aaaaagtacg acatttttac caaaatcaaa aaagagcata ttgatcacta ccttcaagat
     7441 aaatctctag aagcggtatt gactattgat aaagggttct cagataaagt gttgcaagga
     7501 aaatctcaaa aactcaatat acgttcgatt gctaatagcg aaattacaga gtgggtaaaa
     7561 gcacagacca attatctatt ggaaaactat aatattatag gggatgtggc tctaggtaac
     7621 gaagatactt ttaatagaat tctccaaaaa aaccaacagt taaattatga tgttaagcaa
     7681 gtaactttga cagaccgatc acgtagcaaa gcagtatctt ccacaacgac aggatttttg
     7741 cttattctaa tgttaggtag tacgagcgtg atttacagtg gtattttggc agataaatct
     7801 agtcagttat accatcgatt aatgttatcg aacttatctc gttttcgcta tatgttgagt
     7861 tacgtctgtg taggatttgt tgcttttacg attcaaattg tcattatgct gagtttgtta
     7921 aaagtattta atattagttt ttttgttccg acatcacttc ttttgattat ttttttcctt
     7981 tttagtttat tagcgatagg ctttggcctg ctgattggag ctatcacaca aaattcccaa
     8041 caaagtagcc agttagctaa tcttattgta atgccgactt caatgttggc tggctgttta
     8101 tggccattgt ctattacgcc ttcttacatg caagcaattg gtaagttatt accacaaaat
     8161 tgggttttat cagccatagc tatttttcaa agtggaggga ccttatctca ggcttggcca
     8221 tacttgttag ctctaatggg aacagcacta gctttgatta ttttttcaag tttattatta
     8281 aaacctacca gactataatt agtcttttgt taaaaaatac tttcctaatt agctaactac
     8341 caggtagccc ttttattggc ttatatgcaa taaaagggtt gctttttgct tatattttct
     8401 ctagtgcact tatcttttgt taaaagcgat tgcgatgtta ctgttgttac tgtgtttttt
     8461 agagagcata ttatcaaaag cgacctctct agaaagttcg tttttgttga taaatgagtt
     8521 gtcaaagtat tttaaaaaaa cgttttcaat aaacataaaa actaatgtta ttaaaaaaag
     8581 gtgtaaaaac gtatttattt tatgaaatgt ttttgttaca ataagcatgt atcgaatgct
     8641 ataaagctgt cttgttcgct atcaggggtg cttgtgatgt tatgtcagat caaacaagtc
     8701 tttaattggg tgacttaaca gattaaaaga aaagatctaa tcgtgttact tgatagtata
     8761 attaatgcaa acagaaagtg tatcccaatt catagtctca ggtggaggtt aatattcaaa
     8821 aataaaatct tgttgttgtc ttaaactaat cacattaaaa ggagatatca tgattaacaa
     8881 gaaatatata atacctgttt cattgttgac actagctatt acgcttacta gtgttgaaga
     8941 agttacttca cgccaaaatt tgacttatgc caatgaaatc gtaacacaaa ggccaaagag
     9001 agaatctgtt attagtgata aatcgaattt tcccgtcata tcaccttacc tagcaagtgt
     9061 ggattttggt gagagaaaaa catctttgcc aacacctgat aaaggagtaa aagtaactac
     9121 tgaacagtct attgctcaag taagaaaggg gcctgaagaa agaacctata ctgttactgg
     9181 caagattacg agtgtgatca atggctgggg aggctatggc ttttatattc aagatagtga
     9241 aggtattgga ctttatgttt atcctcaaaa agatttagga tacagtaagg gagatattgt
     9301 tcaattaaca ggtacactta ctcgctttaa aggtgattta caactccaac aggtgactgc
     9361 acacaaaaag ttagagttat cttttccgac ttctgttaaa gaagcagtaa tatcagaatt
     9421 agaaacaaca acaccctcaa cattagttaa gttatctcac gtgacagttg gagaattatc
     9481 aactgatcaa tataacaaca catctttcct tgtaagggat gatagtggta aaagtatagt
     9541 tgttcatata gatcatcgta caggggttaa aggggctgat gttgttacta aaataagtca
     9601 gggtgatttg attaacctca cagccatatt gtctattgtt gatggtcagt tacaattaag
     9661 accgttttct cttgaacaat tggaagtggt taaaaaggtc acaagctcaa atagtgatgc
     9721 ttcatctcgt aatattgtga aaataggcga gattcaagga gctagtcata cgtcgccact
     9781 tctcaaaaaa gcggtcaccg tagaacaggt tgttgtcact tatttagacg attccactca
     9841 tttttatgtt caagatctta atggtgatgg tgatttagcg acttcagatg gtattcgtgt
     9901 ttttgctaaa aacgctaagg ttcaagtcgg cgatgttttg accatttcag gtgaagtgga
     9961 agaattcttt ggtcgtggtt atgaggaacg taagcagact gaccttacca tcacccaaat
    10021 tgtggctaaa gcagtgatca aaacagggac agctcaagtt ccatcaccgc ttgttttagg
    10081 gaaagatcgt atcgcgccag ccaatattat tgataatgat ggcttgcgtg tgtttgatcc
    10141 agaagaagac gctattgatt attgggaatc aatggaaggc atgttagtgg cggttgatga
    10201 tgctaaaatc cttggtccaa tgaaaaataa agaaatttat gtcttacctg gctctagtac
    10261 aagaccgtta aataattcag gtggagtatt acttccagct aattcttata acacagatgt
    10321 gattcctgtt cttttcaaaa aaggcaaaca aattattaaa gcaggagact cttacaaagg
    10381 aagattagct gggccagtat cttatagcta tggtaattac aaggtctttg ttgatgacag
    10441 caaaaacatg ccaagtttaa tggatggtca tctaaaacct gaaaaaacaa acttgcaaaa
    10501 agaccttagc aagttaagca ttgcttctta caatattgaa aacttctcag ccaatccttc
    10561 ttcaactaaa gatgagaagg tcaaacggat tgccgaatcc tttattcatg atctgaatgc
    10621 tccagacatt attggattaa ttgaagtcca agataataat gggccgactg atgatgggac
    10681 aacggatgcg acacaaagcg cacaacgcct cattgatgct attaaaaaac taggtggccc
    10741 aacttatcgt tatgttgata ttgctccaga aaataatgtt gacggaggtc aaccaggtgg
    10801 taatattcga acaggattcc tttatcaacc agagcgcgtc agcctttctg ataagccaaa
    10861 aggcggtgct cgtgatgctc taacttgggt taatggagaa ttaaacctta gtgttggtcg
    10921 aattgatcca actaacgccg cttggaaaga tgttcgtaaa tcactagcag cagaatttat
    10981 cttccaaggt cgtaaagtcg ttgttgttgc aaatcatttg aactctaagc gtggggataa
    11041 tgctctttat ggttgtgtgc aaccagtcac ttttaaatct gagcaaagac gtcacgtctt
    11101 ggctaatatg ctagcacaat ttgcgaaaga aggggcaaaa caccaagcta atattgtgat
    11161 gctaggtgac tttaatgatt ttgaattcac aaagacgatt caattaatcg aagaaggtga
    11221 tatggttaac ttggtgagcc gacatgatat ttcagatcgg tattcttatt ttcaccaagg
    11281 caataatcag acccttgata atatattagt ttcacgccat ttacttgatc actacgaatt
    11341 tgacatggtt catgtgaatt ccccatttat ggaagctcac ggacgcgcat cagatcatga
    11401 tccattgtta cttcaattat cattttccaa agaaaatgat aaggcagagt cttctaaaca
    11461 aagtgtaaaa gctaaaaaaa cttcaaaagg aaaactgttg ccaaaaacag gagatagtct
    11521 tgtttatgtg ataacgctac taggaacggc tagtttatta gtgcctattt tattattgac
    11581 taaaggcaaa aaggaatcat agcgatttga agaattggag ttcataaaga gtcgttaaag
    11641 gcttgatata cttatgttag accttaagct aaaatggatg actatataaa aactaatatt
    11701 gataacaaaa gaagataaca ataagacagc tttacttgct aaagactttt gtcaagctaa
    11761 ttgttttatg gtacaatgta accgttgctt tgcaaccctt ggtgcttagt tccctttcac
    11821 caagcatatt agaaacgtag atagcttttg agagttatcc agacgttaaa ggagaaaaaa
    11881 tgacaaaatt gactgtacac gactatgttc acattgggct agtagctgcc ttgtatgttg
    11941 tcttaactat taccccgcct ctaaatgcga tttcttatgg gatgtatcaa tttagaatat
    12001 ctgaaatgat gaatttctta gctttttatc atcgcaagta tattattgcg gtgactcttg
    12061 gatgtatgat tgctaacttt tatagtttcg gactgattga tgtctttgta ggcggcggtt
    12121 caactcttat ttttgtgacc ttaggtgtta ttttgtttag taagtatcaa aaagattatc
    12181 tttttaatgg catttttaat aaagcttttg tttacttttc tttctttttt gcaacttcca
    12241 tgtttactgt agcaatcgaa ctttacttct ttggagctcc atttttatta acatggttta
    12301 caactgcgtt gggagaatta gtatctctgc tcataggatc acttattatt gataagttgt
    12361 ctcaaagaat ttccttttag taatgcatca tactcaaaag agttgggtca atacccttct
    12421 cttttttatg ttgtttgtaa tgttaaaatc gttctagata gaaatattta acactttagc
    12481 taatagtacc atatggagtg gccaacgatt acttttgatg ttgttatgat atagaaatag
    12541 ctcatttgtt atcttttatg ataaaataaa agtatgaaaa aacgcataaa agaattaaca
    12601 gatcttctta accgttaccg ttatgactat tatactaaag atgccccatc agtttctgac
    12661 agtgactatg ataagcttta tcgtgaactg gtgactttag agcagtcata tcccgagtat
    12721 gtcttgcaag atagcccaac gcagcaggtt ggagggacta ttttaaaggg gtttgaaaaa
    12781 tatcgacatc agtatccgct ttttagtttg caggatgctt tttcacgtga ggagctggat
    12841 gcttttgata agcgcgtcaa ggccgaattt ccgaatgcca cctatctagc agagttaaaa
    12901 attgatggtt tatctatttc cttgagttat gagaatggct tcttacaggt tggagcaaca
    12961 cggggtgatg gcaatatcgg tgaaaatatc acagaaaata ttaaaaaaat aaaggatatt
    13021 cctcaccaat tgagtgagcc tttaaccatc accgttcgag gagaggctta catgtctcgt
    13081 cagtccttta aagcgatcaa tgaagcacgc caagaaaatg gggagactga atttgctaat
    13141 cctagaaatg ctgccgctgg gacactaagg cagctagata cggcagtggt tgctaagcgt
    13201 caactagcca cttttttgta tcaagaagct agtccgacag ctagaaatca acaaaatgag
    13261 gtcttagcag aattagcaga tctaggtttt tcagtaaatc cgtattatca actcacatcg
    13321 tctatggatg agatttggaa ttttatcaaa actattgagg caaaaagaga tcaattagct
    13381 tatgacatag atggcgttgt tattaaagtt aatagcctag ccatgcaaga agagctaggg
    13441 tttacggtta aagctccacg ctgggctatt gcttacaaat ttccagcaga agaaaaagag
    13501 gcagaaatcc tttcggttga ctggacagtg ggacgaacag gcgtggtcac acctacagcg
    13561 aatctaactc ctgtgcaact agcggggaca accgtgagtc gtgcaaccct acataatgtg
    13621 gattatattg ccgaaaaaga tatccgtatt ggcgatacag tgattgtata taaggcaggg
    13681 gacattattc ctgctgtctt aaatgtcgtt atgagtaagc ggaaccagca agaagtcatg
    13741 cttattccta aactatgccc atcttgtggt agtgaattag ttcattttga agatgaggtt
    13801 gctttgcgtt gtattaatcc gttgtgtcct agtttgattc agcgaagttt ggagcatttt
    13861 gcaagtcgtg atgccatgaa cattactggg ctgggaccag ctattgtgga aaaactattt
    13921 ttagcggggt ttgttcatga tgttgctgat atttaccagt tgaccaaaga agatttcatg
    13981 caattagatg gcatcaaaga aaagtcagct gataaattgc tcgctgcgat tgaggcttct
    14041 aaatcaaatt cagctgaaaa actgttattt ggtctgggaa ttcgtcatat tggctctaag
    14101 gttagtcgct taattttaga ggtatacgga gatatttctg ctcttttaac cgctaaagaa
    14161 gaggaaatcg caaggataga tggtcttggc tcaacgattg ctcaatcact tactcaatac
    14221 tttgaacaaa aaacggctgc gattttagta gacgagttaa agacagcagg tgtcaacatg
    14281 cattactcag gtcaaaaagt taatagtgac gcagcgcttt ttggtctaac tgttgtttta
    14341 actggcaaat taaatcagct gaaccgaaat gaggccaaag ataaattaga ggcattaggt
    14401 gccaaagtta ctggaagtgt atctaagaaa acagatttag ttattgcagg tagtgacgct
    14461 ggttctaagc tagaaaaagc aaagagctta ggaattcgga ttgaagatga agattggcta
    14521 cgaaagcttt aagtgaggta atgttatgaa aaaacaattg agagcacgtc tcatttataa
    14581 tccaacgtca ggacaagaac ttatgagaaa aagtgttcct gaagttttag acatattaga
    14641 agggttcggg tatgaaacat ctgcctttca aacaacagcc caaaagaact ctgctctaaa
    14701 tgaagcaaga agagcggcaa aggcaggttt tgacttattg attgctgctg gaggtgatgg
    14761 cactattaac gaagtggtca atggaattgc tcccttgaaa aagcgcccta aaatggccat
    14821 tattccaaca ggtaccacta atgattttgc tagagcgtta aaagtaccaa gaggaaatcc
    14881 gagccaagca gcaaaactta tcggaaaaaa tcagactatc cagatggata ttggtagagc
    14941 taagaaagat acttatttca tcaatattgc tgcagcagga agtttgacgg aattaactta
    15001 cagtgtacca agtcagttaa aaacgatgtt tggatacttg gcttatcttg ctaaaggagt
    15061 agaactgctc ccaagagtta gcaatgtgcc tgttaaaatt acgcatgaca aaggcgtctt
    15121 tgaaggtcaa gtttctatga tttttgcagc tattaccaat tctgttgggg gatttgaaat
    15181 gattgctcct gatgcaaaac tagatgacgg tatgtttacg ctcattttaa taaagacagc
    15241 aaatcttttt gaaattgtac atcttttgcg tttgattttg gatggtggta agcatattac
    15301 agatcgtcgt gtcgaataca ttaagaccag taaaatagtc attgaacccc aatgtggtaa
    15361 gcgaatgatg attaatttag atggcgagta cggaggagat gctccaatta cattagagaa
    15421 cctaaaaaat catatcactt tttttgcaga tactgatttg atttctgatg acgctttagt
    15481 cttagatcag gacgaattgg aaattgagga aatcgtaaag aaatttgctc atgaagtaga
    15541 agacttagaa caagaattag aagaataata caatagttat tagctactga cagtttagtc
    15601 aaatgcacta ctattagaat ctaagatttt taaatcaatt gaggtagtgt tttttgatgt
    15661 gctcaaagag tcaatttatt catatgaggg gcctatttga ggtctctctt ttttgatcat
    15721 atgcaagaaa atgccatgtt ttaggggtgt tattataccc tttttggaat cttgttgcct
    15781 ttttgttata ataagagtgt aattctattt ttaaaaagat cttaacaaag acagactcgt
    15841 ttatcaaaaa ggttagtatt agtctaagaa agcgtactca gtctaaagaa atctgttgac
    15901 attcttaatt ttaaggaata gaatagcaaa gtaaaaaaaa tttaaaggag tattctacta
    15961 tgaatcctat tttcgcattg gctttggcct gttttggtgt ttctcttgcc gaaggtttct
    16021 tgatggcaaa tctttttaaa gcagcatctc gccaaccaga aatcattggt caattacgtt
    16081 cattaatgat cttgggtgtt gcctttatcg aaggtacttt ctttgttacc cttgttatgg
    16141 ccttcattct taaataacag cctatttgat aatagaaaag gagggtgtta gatggaagaa
    16201 gctaaaatac ccatgctaaa gcttggtcca ataaccttta atttgaccct acttgctgtt
    16261 tgtattgtca caattgctgt tatctttgcc tttgtttttt gggcaagtcg ccaaatgaaa
    16321 ttgaaaccag aagggaagca aactgcttta gaatatttaa tcagttttgt ggatggtatt
    16381 ggagaagagc acttagatca taatctacaa aaatcttatt cgctgttact ctttaccatt
    16441 tttctctttg tggctgtcgc taataattta gggttattta ctaagttaga gacagttaat
    16501 ggctataacc tatggacctc gccaacagcc aatctggctt ttgaccttgc tctatctctt
    16561 tttattacct taatggtaca cattgaaggg gttagacgtc gtggcttggt tgcccatttg
    16621 aaacgtttgg ctacaccgtg gccaatgact ccgatgaatt tattagaaga gttcacaaat
    16681 ttcttatcac ttgccattag gttattcggt aatatctttg ccggggaagt tgttacaggt
    16741 ttgattgttc aactggccaa ttatcgagtt tattggtggc cgatcgcttt cctagtcaat
    16801 atggcgtgga cagccttttc agtctttatt tcctgcatac aggcttttgt tttcacaaaa
    16861 ctaacagcga cctatctagg aaagaaagtt aatgaatcag aagaataagg aaggagaaca
    16921 aacgaatgtc aatcacattt ggtgaactcg ttgggaattt tattctagta acaggttctg
    16981 taatcgtctt gttactcttg atcaaaaaat tcgcttgggg tgcaattgaa tcgattttac
    17041 agacacgctc acaacaaatc tctcgagata ttgatcaggc tgagcaatca cgtctaagtg
    17101 ctcaacagtt agaggcaaaa agtcaagcta acctagatgc tagtcgttca caagcaagta
    17161 aaatcattag tgatgccaaa gaaattggtc aattacaagg tgataaattg gtggcagaag
    17221 ctactgatga agcaaaacgc ttgaaagaaa aagcgttgac agatattgaa caaagcaaat
    17281 cagacgctat ctcagcagtc aaaacagaaa tgtctgattt aacggttctt ttagcggaaa
    17341 aaattatggg agccaatctt gataagacgg cgcaaagcca gcttattgac agttatcttg
    17401 atgacttagg agaagcttaa tgaccaaaaa agaacaggct attatcgagc aatatgcaaa
    17461 aagccttgtt gaggtagcaa gtgaacatca ttctcttgat accttacaag ctgatgtgct
    17521 tgcgatttta gagacgtttg aaacaactaa tcttgatcaa agtctatcaa gtctagctgt
    17581 tccgcatgct gaaaaaataa aattgctaac cttattaaag ggaaataatt ctgtttatat
    17641 gaataatttc ttgaatctca ttttacaaaa cgaacgtgaa gcttatttgt atcaaatgct
    17701 tcaggctgtt ttaaatgaaa ttgcaatagt ttcaaatcag tatgatgtga ctgttacctc
    17761 atcactacct ttgactgagg agcaaaaaaa ccgtgttcga gcagttgttg caaagaaatt
    17821 tgctgtaaca gcgggtcgtt taattgaaaa agtagatcct tcgcttattg gaggatttat
    17881 tatcagcgtc aataataaag taattgatac cagtattcgt cgtcaattgc aagcatttaa
    17941 aatgaattta aaatagaaag tggtgtgact tttggcaatt aatgcacaag aaattagcgc
    18001 tttaattaaa aagcaaattg agaacttcca gccaaatttc gatgtcacag aaactgggat
    18061 cgttacctat attggtgacg gtattgcacg tgcccgtgga ttagataatg ccatgagtgg
    18121 tgaacttctt gaatttgaaa atggtgccta tgggatggct caaaaccttg agtcaaatga
    18181 tgtgggtatt attattcttg gtgatttttc agcaatccgt gaaggagatg tggttaagcg
    18241 caccggtaaa atcatggaag taccagttgg tgaagctctc attggacgtg tggtaaaccc
    18301 acttggacaa ccagttgatg gcttgggaga tattgaaaca actggcttta gaccagtaga
    18361 aacgccagct cctggtgtta tgcagcgtaa atccgtttct gaacccttac aaactggatt
    18421 aaaagcaatt gatgctttgg ttccaattgg tcgtgggcaa cgtgagttga ttatcggaga
    18481 ccgtcaaaca ggaaaaacat ccgtagctat tgatgcgatt ttgaaccaaa aaggtcaaga
    18541 catgatttgt atctatgtgg ctattggtca aaaagaatca accgttcgta cacaagttga
    18601 aacccttcgc cgttacggtg ccttggatta tactattgtt gtgacagcgt cagcctcaca
    18661 accatcacca ttgctcttca tcgctcctta tgcgggtgtt gccatggctg aagaatttat
    18721 gtaccaaggc aagcatgtat tgattgtcta tgatgattta tcaaaacaag cggtggctta
    18781 tcgtgaattg tctctcttgc ttcgtcgtcc accaggtcgt gaagcttacc caggggatgt
    18841 tttctacttg catagccgtc ttttggaacg ctctgcaaaa gtttctgatg acttaggtgg
    18901 aggttctatc acagctctac catttatcga aacacaggcc ggagatatct ctgcatatat
    18961 tgcgactaac gttatttcga ttacagacgg acaaatcttc ttgcaagaga acctctttaa
    19021 ttcaggaatt cgtccagcga ttgatgcagg atcatctgtc tcacgtgtag gtggttcagc
    19081 tcaaattaaa gcgatgaaaa aggttgctgg tactcttcgt ttggatttgg cttcctatcg
    19141 tgaattagaa gcctttaccc aatttggttc ggaccttgat gctgcaacac aagcaaaact
    19201 taatcgtggt cgccgtacgg ttgaaatctt gaagcaacca ttgcataaac cgttacctgt
    19261 tgaaaagcaa gtcgttattc tttatgccct tactcatggt ttcttagatg atgttcctgt
    19321 agatgatatt ttggcttttg aagaagctct ttacgactac tttgacgttc attacaatga
    19381 tttatttgaa actatccgta ccacaaaaga ccttcctgaa gaagctgccc ttgatgccgc
    19441 tattaaagcg tttaaagaac actcaaactt taagtcatag agggaggata ctagtatggc
    19501 aggctctcta agtgaaatta aagctaaaat tatttcaact gagaaaacaa gtaaaatcac
    19561 tagtgccatg cgtatggttt cttctgcaaa acttgttaaa tctgagcaag cagctcgtga
    19621 ttttcaaatt tacgcctcaa aaattcgcca gattactaca gatttattga aatcagaatt
    19681 aaccatagga tctgataatc cgatgttggt gtctcgtcct gtcaaaaaaa caggctatat
    19741 tgtcatcacg tctgataaag gacttgttgg aggttacaat tcaaaaatct tgaaatctgt
    19801 tatggatatg attacagagt accatgctga tggtgactat gaaatcattt ctattggtag
    19861 tgttggttca gacttcttca aagcaagagg gatgaatgtg gcttttgaac ttcgtggact
    19921 tgcggatcaa ccaagttttg aacaagtgag acaaatcatt tcacaatccg ttgacatgtt
    19981 tgtcaatgag atttttgatg agttatatgt ctgttacaat caccatgtga atagcttaac
    20041 cagccaagtc cgtgttcaac agatgttgcc aatttctgac ttggttgctg atgaagcagc
    20101 agaagaaggt gttactgggt ttgaattgga acctaatcgt catgatattt tagatcaatt
    20161 gttacctcaa ttcacagaaa gtttaattta tggggctatc attgatgcta aaacggctga
    20221 acatgcagca ggtatgactg ctatgcagac agcgacagat aacgctaaga atgtcattaa
    20281 cgatttgacc atccaatata accgagctcg tcaagcagca attacacaag aaattactga
    20341 aattgttgca ggagcaaatg cactagaata aaaaataacg ctgactagcg attaggttat
    20401 tgtcagcatt caattttatc aatcgagata aaatgactta tgggactttt ctattgaaag
    20461 actgctcttt tcagtataaa agaagttagc aagactaact aagccctgac tatcgaatat
    20521 aaggagaaat gaatgagctc aggcaaaatt gctcaggttg ttggtccagt agtagacgtc
    20581 atgtttgcta gtggggacaa acttccagag attaataatg cattgattgt ttataaagat
    20641 agtgataaaa agcaaaaaat cgtccttgaa gttgcactgg aacttggtga cggtatggtg
    20701 cgaacaatcg ctatggaatc aactgatggg cttacacgtg ggttagaagt tcttgacact
    20761 ggtcgtgcga ttagcgtacc agtaggtaaa gaaactttgg gacgcgtctt taatgtactt
    20821 ggagaaacca ttgatttgga agaaccattt gcagaagatg ttgaccgtca gccgatccat
    20881 aaaaaagcac catcgtttga tgaattatca acatcatcag aaattcttga aactggtatc
    20941 aaggtaattg accttcttgc cccttacctt aaaggtggta aagttggact tttcggtggt
    21001 gccggagttg gtaagactgt ccttatccaa gaattaatcc acaatatcgc ccaagaacac
    21061 ggaggtattt cagtatttac cggtgttggt gagcgaacac gtgaaggaaa tgacctttac
    21121 tgggaaatga aagaatcagg cgttattgaa aaaaccgcca tggtttttgg tcagatgaat
    21181 gagccgcctg gggcacgtat gcgtgtagca cttactggtt taaccattgc tgagtatttc
    21241 cgtgatgtag aaggccaaga tgttttgctc tttattgata atatcttccg tttcactcag
    21301 gcaggttcag aagtatcagc cctcttaggc cgtatgcctt ctgctgttgg ttaccaacca
    21361 acccttgcta ctgaaatggg gcaattgcaa gaacggatta catcaactca aaaaggatct
    21421 gttacatcta tccaagcgat ttatgtacca gcagatgatt atactgaccc tgcgccagca
    21481 acagcatttg ctcacttgga ttcaacaact aaccttgaac gtaaattgac tcaaatgggt
    21541 atttatccag ccgttgaccc attggcttca agttcacgtg ctttatcacc tgagattgtt
    21601 ggtgaagagc actatgctgt ggcaacagaa gttcaacgtg tgcttcaacg ctaccgtgaa
    21661 ttacaagaca tcatcgctat tttggggatg gatgaattgt cagatgaaga aaaaacattg
    21721 gttggacgtg cgcgtcgtat ccagttcttc ctctcacaaa attttaacgt tgctgaacaa
    21781 tttaccggtc ttcctggttc ttacgtgcca gttgctgaga cagttcgcgg ctttaaagaa
    21841 attcttgaag gaaaatacga tcaccttcca gaagacgcct ttcgttcagt tggtccaatc
    21901 gaggatgtga taaaaaaagc cgaaaaaatg ggcttttagt gaggtgatgc catgacacaa
    21961 atgactgttc aagtggtaac gccagatgga atcaaatatg atcaccatgc taaatgtatt
    22021 tctgtaacaa caccagatgg ggaaatgggg attttaccta accacatcaa tttaattgca
    22081 cccttgcaag ttcatgaaat gaaaattcgc cgaggtggtg aagatgaaaa agtggactgg
    22141 attgctatca atggtggtat tattgaaatc aaggataatg tggttactgt tgtcgctgat
    22201 tctgcagaac gtgaccgtga tatcgatgtc agccgagcag agcgagcaaa actaagagca
    22261 gaacgtgaaa ttgcacaggc tgaaacgacc cacaacattg atgaagttcg tcgtgccaag
    22321 gttgccttgc gccgtgcttt gaatcgtatc aacgttagta aaaaataagt ggtgttgata
    22381 aaagacaagg ataagtaaga aaggttttta acctaatatt ttgtttgctg agtcttttta
    22441 acatcagttt ctgagagttt gaccatacta ccttctctaa acacgttatt attgttgaga
    22501 ggagttatat gggggaactc ttttttgtgt ccacgttgtt agtagaaact cagtaatgac
    22561 gcttaatata tttttaggct atcaacttag tcagagagga tgtcaactta attgataagt
    22621 tggacggatc aagatggtat gttataataa tcctatggaa ttattaatga agatcattgc
    22681 cttttcgtgt catttatttt ttattggcct tagttaccaa ttattgatta gtgtgattga
    22741 ctggacaaaa tttagtcatt atcatccaga aaatcttggc cggctacgac tttttgtctt
    22801 tttattagct actgccctag ggtatttggt gagccattgt atgctggaat tgcttcaaat
    22861 tagccagacg ctcttttttg agttccgcta atactggaat aaaagaagta tggtataatt
    22921 ttactacgac taagagatag ataagggaga aaattgtgga taaaataatc attgaaggtg
    22981 gtcaaacacg cttagaagga gaagttgtta ttgagggtgc taaaaacgca gttcttcctt
    23041 tactggcagc atctatttta ccttcaaaag gaaaaacaat tcttagaaac gttcctattc
    23101 tatctgatgt ttttaccatg aataacgtgg tacgtggttt agatattcgt gttgatttta
    23161 atgaagcagc taacgaaatc acagttgatg cctcaggaca cattttagat gaagcccctt
    23221 acgagtacgt gagccaaatg cgtgcctcta ttgttgtttt aggaccaatt ttggcacgta
    23281 atggccacgc aaaagtatca atgcctggag gctgtacgat tggcagtagg cctattaatt
    23341 tgcacttaaa aggattagag gctatggggg caacgattac tcaaaaaggt ggagatatta
    23401 ctgctcaagc agatcgactg caaggtgcca tgatttatat ggacttccca tctgtaggag
    23461 ctacccaaaa cttgatgatg gctgccactt tagctgatgg tgtgaccact attgaaaatg
    23521 ctgctcgtga gcctgaaatt gttgatttag ctcaattttt gaataaaatg ggagctagaa
    23581 ttcgtggtgc tggaacagag acgttaacga taacaggcgt cacgcattta cgaggcgtag
    23641 aacatgatgt tgttcaagat cgtatagaag ccggtacatt tatggttgca gcggcgatga
    23701 cttcaggcaa tgttttgatt agagatgctg tttgggaaca taatcgtcca ttgatttcaa
    23761 aattaatgga aatgggtgtt tctgtaacag aagaagaata cggtattcgc gttcaagcaa
    23821 acacgccaaa gctcaaacct gtaacggtta aaaccttgcc tcatccaggt tttccaacag
    23881 acatgcaagc tcaatttaca gctttaatgg cagtggtcaa tggagagtct actatggttg
    23941 aaactgtctt tgaaaatcga tttcaacatc tggaagaaat gcgacgcatg ggcttacaat
    24001 ctgaaatcct tagggaaact gccatgattc atggtggaag acaactgcaa ggtgctcctg
    24061 tgatgtcaac agatttacgt gccagtgctg cccttatctt gacagggatt gttgctcaag
    24121 gagttacaat agttaataat ttggttcatt tagatcgtgg ctactatcaa tttcatgaaa
    24181 aattagcaaa gcttggagct actattagtc gtagcagtga ggtctagctt atgacaaatg
    24241 gtttaaaata tgtcttagaa caaatgttat tacttttcat aattgctgcc ttggcgtgtc
    24301 tttttttagc cattggactt atgattggtt atagttttat gggagatggc cagagtccat
    24361 ggcatatttt atctatggat aagtgggcag agttagtgaa taaatttact ggaaagtagg
    24421 ccttgcctac tttttatagc atctgagagg aagtatgtta acaaggaaaa aacgaaaaag
    24481 ggtgcaagag caaaaggttc ctaaaagagc taatgcctta cttactaagt cgacacgaca
    24541 gtatcgaaat cgctatcaaa cggataatgc ttatcgtacc ttcaagccag caggttggca
    24601 ccgattgcac cacttaaaag gaagttatga tcacgcggtt gatagagggc atttgctagg
    24661 ttatgcttta gttggtggac tgaaaggttt tgatgcttct accggtaatc ctgataatat
    24721 tgccactcaa ctaagttggg ctaatcaagc aaataaaccc tactcgacag gtcagaatta
    24781 ctatgaaggt ttagtatgtc gtgctttaga taaggggcat cgcgtccgct accgtgtcac
    24841 cctactttat gatggtgata atctactagc cagcggtagc catttggagg ctaaatcctc
    24901 tgatgacagc ttgactttta acgtgtttgt tccaaatgtc caagcaggat tgaccgttga
    24961 ctatcggacg ggacaaatag ctatcaatct ctaagcgaaa agtcactgat ttaagattgt
    25021 cactaagcac tttttttgat agaatagaac taattgaata agtgtctatg agactagtac
    25081 taatagagaa attaacaggg agtgagggcc gtgactggaa gccttcttaa gggaactatt
    25141 aggaattcac tcagctaaag acaattaatt aaggtctgat aacatcagat aaaaacggat
    25201 ggtaccgcgt gtcaacgctc cgcttatcaa gtggaggagt ggcacgcttt tttcatcgct
    25261 aaaagaaggg aaaactatgg atttacaagc acaattagag gaattaaaga caaaaacatt
    25321 ggagacctta cagtctttga cgggtaatca taccaaagaa ctgcaagatt tgcgcgtggc
    25381 tgttcttggg aaaaagggat ctttgacaga attactaaag gggctaaaag acttgtctaa
    25441 tgacttgcgt cctgtggttg gtaaacaggt gaacgaagtt cgtgacttat tgacaaaagc
    25501 ctttgaagaa caggccaaaa ttgtagaagc tgctaaaatt caggcccaac ttgatgctga
    25561 aagcattgat gtgactttac cgggtcgtca aatgactttg gggcatcgtc atgtgttgac
    25621 acaaaccagt gaagaaatcg aagatatttt cttaggaatg ggttttcaga ttgttgatgg
    25681 ctttgaggtg gaaaaagatt attacaactt tgagcgcatg aatttgccaa aggatcaccc
    25741 tgcccgtgac atgcaagata cgttttatat cacagaagaa atcttgttac gaactcacac
    25801 cagtcctgtg caggcccgta ctcttgatca acatgatttt tcaaaagggc ctttaaaaat
    25861 ggtttctcca ggtcgtgttt tccgtcgtga tactgatgac gctacccact cacatcaatt
    25921 ccatcaaatt gaaggtttgg ttgtgggcga aaatatttcc atgggagacc tcaaagggac
    25981 ccttgaaatg atcatcaaga agatgttcgg tgacgaacgt agcattcgtt tgcgcccgtc
    26041 atatttcccg ttcacagaac catctgttga agtggatgtt tcttgcttta aatgtggtgg
    26101 gaaaggctgt aatgtctgca agaaaacagg atggattgaa attcttggtg ctggtatggt
    26161 tcacccaagt gtcttggaaa tgtcaggtgt tgatgcaaaa gagtattcag gctttgcctt
    26221 tggtttaggt caagagcgga ttgccatgtt gcgttatggc attaatgaca ttcgtggttt
    26281 ctatcaaggc gaccaacgtt tctctgaaca atttaactaa aacgagtcgt ttgaagacct
    26341 ctgtcagcta ttttatctaa gattgagtga aagttgatag gtaatgataa taggttaatt
    26401 gtctaaggag aaagtccctc tcgcttcaac gtcataaggg ttttgacttg gaacttaaaa
    26461 tacgaaagga aatgactcaa atcacggctt gagagctttc tctagccgag tgatttgggc
    26521 actaaaatta tgttagtatc ttataaatgg ttaaaagaat tagtagatat cgatgtgaca
    26581 ccggcagcac ttgctgagaa aatgtcgaca acgggtatcg aggtagaagg aattgaagtc
    26641 cctgcagatg gtttatcaaa actggttgtg gggcatgtgc tttcttgcga agatgtgcca
    26701 gagacacatt tgcatttgtg tcaggtagat acaggtgatg aaacgccacg tcaaattgtg
    26761 tgtggcgcac caaatgtgaa agcagggatt aaggttatcg tggctgtgcc aggtgctcgt
    26821 attgcggata actataaaat caaaaaaggc aaaatccgcg gcatggaatc tcttggcatg
    26881 atttgttcct tgcaagaact tggtttgtca gatagtatta ttccaaaaga attttcagat
    26941 ggtattcaaa tcttgccaga ggaggctgtg ccaggtgatg ctatttttaa atacctggac
    27001 ttggacgatc atatcattga attgtccatt acacctaacc gtgctgatgc gctttctatg
    27061 cgaggtgtgg cacatgaagt cgcagctatt tatggcaagt cagtatcatt cccccaaaaa
    27121 aatctacaag aatctgataa ggcaacttca gaagccattg aagtggccat tgcctctgat
    27181 aacgttctaa cttacgctag ccgtgtcgtt gaaaatgtca aagttaagcc aagtccacaa
    27241 tggttgcaaa accttttaat gaatgctggt attcgtccta ttaataatgt ggttgatgtg
    27301 accaactatg tgctcttgta ttttggtcaa cctatgcatg cttttgatta tgataaattt
    27361 gaagaccata agattgtggc gcgtgcggct cgtcaagggg aaagtttagt cacacttgat
    27421 ggtgaaaaac gggatttgac cactgaggat cttgttatta ccgtggctga caaaccagtt
    27481 gctttggcag gtgttatggg tggtcaagcc actgaaattg atgccaactc tcagacggtt
    27541 gttcttgaag ttgctgtttt tgatggcaaa tcgattcgta aaaccagtgg tcgtttaaac
    27601 cttcgttcgg aatcctcttc acgttttgaa aaaggggtta actatgcaac tgttttagaa
    27661 gcgcttgact ttgcagcggc tatgttacaa gaattagcag aaggtcaagt cttatcaggc
    27721 catgttcaag ctggacaatt gccaacagaa cctgttgaag tctcaactag ccttgactat
    27781 gtgaatgttc gtttaggaac agaattaact tttgctgata ttcaaaggat ttttgaccaa
    27841 cttggatttg gtctaacagg agatgaaaca agcttcacgg tagctgttcc tcggcgtcgt
    27901 tgggatgtta gtattccggc tgatttggtg gaagaaattg cgcgtattta tggttatgat
    27961 aagttgccaa caaccttacc agaggtagga ggaacggctg cagaattaac gccaacacag
    28021 gctcttcgtc gtaaggtccg tggtttagcg gaaggattgg gcttgactga aatcatttcg
    28081 tatgctttga caacaccaga gaaagctgtt gagtttgcag tggcaccaag tcacttgaca
    28141 gaactcatgt ggccgatgtc tgtggaacgt tcagcgcttc gtcaaaatat ggtttcaggg
    28201 atgctagata cggttgccta taatgtggct cgtaaacaaa gtaacttggc tctttatgaa
    28261 attggaaaaa tctttgaaca agaagctaat ccaaaagaaa accttcctaa tgaagtgaac
    28321 cattttgctt ttgctatttg tggcttagtg gcacaaaaag atttccaaac ccaagctcag
    28381 gcagtggatt tttaccatgc taaaggtatc ttggatactt tatttgcaaa ccttaacctc
    28441 aaggttcaat atgtgccaac caaagaccta gcaagcatgc acccaggacg gacagcgctt
    28501 atcctattag atgagcaagt gattggattt gttggtcaag ttcatccagg aacagcaaag
    28561 gcttatagta ttccagaaac ctatgtggca gaattgaata tggcggcttt agaagcagcg
    28621 ttgtcaagtg atcaaacttt tgtggagatt accaaattcc ctgctatgac acgtgatgtt
    28681 gccttgttac ttgaccgtga agtgagtcat caggccattg taacagctat tgaatcagca
    28741 ggtgtaaaac gcttgactaa gattaagctc tttgacgtgt atgaaggagt aactattcag
    28801 gctggcaaaa aatcaatggc ttatagcctt actttccaaa atccaaatga caatttaacg
    28861 gatgaagaag ttgccaaata catggagaaa atcaccaaat cattgacaga acaagtcggt
    28921 gctgaagtac gctaagaaaa aaaactgggc ctctaaggct ccgttttttt ctgttataat
    28981 agaggaaaaa agaactccag ttcaaatgat tggagaaaaa catcagaagg tggaggaaaa
    29041 tcagaatgta tcaaacaaaa ataacaggca accgccttta ccgcacggtt tcgaaaggct
    29101 acggagatag tgtaacctta tttggaaaaa ctgatcaagg ggaaacccct atgagtttac
    29161 tggttattgc cttgtcttct tgtgtgacta tgtgtgtgca gggtttttac catcgtcaat
    29221 ttcagcaaga cacacttgct gtttctgttg aggctagtta tgaggaagaa gtttttgaac
    29281 tacttgttag gattgatgat gttttagatg acaaaaagaa agctgattta ttggcttaca
    29341 ttggaactta ttgtcgtgtg aaacaactcc tcaaacctga agttacggtg accattacgt
    29401 tggaaggact gtctcatgtc tgatgctagt ttgaaaactt accaaaaaca atgggcttat
    29461 caaaagtatt gggtcatggc acactcacag cagcattaca atgctttaag agaattgttt
    29521 aaaggaaatc agtggtcaga agaaaaggtg ttgacttttc attgtttgat tgaagaagcg
    29581 caagccattc ctcccacagt aaaaagctta cgaacggcat atcagcatgt ttgggggtat
    29641 tttaaaaagg tagcgagtca ggaagaaaaa gaccatttca aagatttgga tgctcagttg
    29701 gaaactaaat ctgaggagat gctatgcttt ttacaagaga tgactgctca ttatcagccc
    29761 tcttatctgc tatcttgccg tttgattacc aaaggtcctt aaatgctaaa cagaaaagta
    29821 gggatttctg ggaagttatg ctaagctagt gatataaata agggggaatg agagatgttt
    29881 ttagcgctta atgaaatgaa acaatccaaa ttgcggtatg gtttgattgc tggtttgcta
    29941 tgtttggttg cctatttgat gttctttttg tcaggattgg cctttggttt gatgcaggag
    30001 aaccgctcgg cggttgatct ttggaaagct gacagtgttt tactagccaa ggatgctgac
    30061 gctaccttga ccttatcaca ggtgtccaga gctcaagaaa atcaaataac agcagacaag
    30121 gtagctcctc ttgctcaact caataccgtg gcgtggtcag ttaaaaatcc taaggatgcc
    30181 gacaaagtta aggttagcct tttcgggatt gattctaata gctttattcg tcctaacatt
    30241 gtaaaaggtc gattatttaa gactaacaaa gaggttgttt tggatcaaag ccttgcaaaa
    30301 gaggaagctt ttgcgattgg caaggacttt tacacatcga gttctagtca agcattaact
    30361 atcgttggtt atactcaaaa tgctagattt agtgttgcac cagtggttta tatgaatttg
    30421 gaagcttttg aaacattaaa atatggagaa ccactaccaa aagataagca agttgttaat
    30481 gcttttatca ctaaaggaag tttaacagat tatcctaaaa aagacttcca aaaattagat
    30541 attaaaacct ttattactaa attacctggt tatagcgctc aacttttaac ttttggcttt
    30601 atgattagtt ttcttgtcat tatttcagct attattattg gtatttttat gtatattttg
    30661 actattcaaa aggcacctat ttttgggatt atgaaagcgc aaggtatcgg gaatataacg
    30721 attgcacaag cagttctgct gcaaaccttc ttgttaagtg ctttaggaag cggtttaggg
    30781 ttattgggaa cttggttgac gtctttagtg ctccctgaag cagttccctt ccaaagtaat
    30841 tggctagttt acttagcgat attagtcagt atggtttgtt ttgctttgtt aggaacctta
    30901 ttttctgttt ttagcattgt acgaattgat cctttgaaag caattggata ggaggtagtg
    30961 atgtcggttt taacatttaa acaagtgacc aaaacatttc aagatggtca tcacgaaatc
    31021 aacgcattaa aggcaactga cttttccatt gaagctggag aatttgtggc tattattgga
    31081 ccatcaggtt ctggaaaatc aacctttttg accattgctg gtggcttaca gaccccaagc
    31141 tctggtcagc taatcattga tggcactgat tatacccatt tatcagaaaa agagcgctca
    31201 cgcttgcgct tcaaatccgt tggttttatc ttacaagctt ccaatctgat acctttttta
    31261 acggtccagc agcagttgga attggtcgat catttgacag gaagcaaaga aaaagccaaa
    31321 gccaatcagt tatttgacga tttaggcatt acagggctaa aacatcagtt acctcaagaa
    31381 ttgtccggcg gagaaaggca aagagcggct attgcccgtg ctctttacca tgatccggca
    31441 ctgatattgg cagacgaacc tactgcaagt ttggatactg aaaaagccta tgaagtggtc
    31501 aaactgttgg ctaaggaaag taaagaaaaa aacaaggcca ttattatggt cactcatgat
    31561 gatcggatgc taaaatattg tgacaaagtc tatcagatgc aagatggaga gttacgtcaa
    31621 gagagatagt agactggtaa gagtttatga tattagaaga aaaaaccaaa caagcacagc
    31681 acttgtctgg tttttttcta agtgtgacta ttatcaatac gtcaccattt ccctaaagct
    31741 tactatacgg gatgctaaag gtatcaccat gctcaaagtc gccatattgg taacctcggt
    31801 caaaccaacg ttggcgttgc ttagaagttc cgtgggtaaa actatcagga acggctctgc
    31861 cataggtttc ttcttgcaga gtatcatccc caactgcgtg agctgctgcc attgcttcct
    31921 caatatcacc tttttccaaa aggccttgcc cttgaacata attagcccag gcaccagcat
    31981 agtaatcagc ttgtaattcc aacttgacgt tgagctgatt ggctttagcc tttgattttc
    32041 cttgtctagc gctagcgtaa ttatccataa ttcccaattc attttggatg tggtggccaa
    32101 cttcatgagc aatcacatag gccatagcga aatcaccttt tgcgccgtac ttggttgata
    32161 gttcattata aaaagaaata tctaaataca ctttttgatc gcctgggcaa tagaaaggac
    32221 cagaagaggc ttgccccctg ccacaagcgg tttgagttgc tccagtatat agaactaatg
    32281 ttggtttatg ataagttaac cctttctcac gaaatgtctt agtccaatag tcctcggtag
    32341 aggcgaagac cttactgaca aaagaaacct gttcttgact agccttgtca ccatttgtac
    32401 gcgttacttg gcttgattga taggcattgt tgttggtaga agaaggcttg cctcctaaaa
    32461 caccagatag gccacctcct cccataacta ggagaaggag aagaataacc agcttggttt
    32521 tccaacctcc tcgtgaaaag agtaattgaa gcaggagacc gccccctagg ccgccgcccc
    32581 caaaagaacc agacgattga ccacgtctat cttcaacctg ttgactttct ctcaaatcat
    32641 ctgttttcat aagtttctcc catctatttc ttgattttat tataaaataa ttctattaga
    32701 attctatttt tatgatgaga ttat
//
LOCUS       DAUGFP010000016        29071 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.016, whole genome shotgun sequence.
ACCESSION   DAUGFP010000016 DAUGFP010000000
VERSION     DAUGFP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29071
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.016"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..313
                     /gene="gpsB"
                     /locus_tag="VH663_000785"
     CDS             <1..313
                     /gene="gpsB"
                     /locus_tag="VH663_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEQ8174003.1"
                     /translation="YSPKDIFEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNAQIEAL
                     KAENEALKKAKFQARNTVSATVQQPVPQPTRIAQSATNFDILKRISKLEKEVFGKQII
                     E"
     gene            327..695
                     /gene="rnpB"
                     /locus_tag="VH663_000786"
     ncRNA           327..695
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VH663_000786"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            758..1912
                     /locus_tag="VH663_000787"
     CDS             758..1912
                     /locus_tag="VH663_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEQ8174004.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWENYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKINGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            1926..3395
                     /locus_tag="VH663_000788"
     CDS             1926..3395
                     /locus_tag="VH663_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174005.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERVIPEPQITKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDVVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQAAQAQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGNQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(3540..4022)
                     /locus_tag="VH663_000789"
     CDS             complement(3540..4022)
                     /locus_tag="VH663_000789"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEQ8174006.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGTTWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(4096..5427)
                     /locus_tag="VH663_000790"
     CDS             complement(4096..5427)
                     /locus_tag="VH663_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEQ8174007.1"
                     /translation="MEIIGSLGVLVGVIVIIYLSVKEVNIIIAAPLATSLVILFNQMD
                     PTKTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGATTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVTFGLFYMKYCLAKSMAMG
                     ETYATYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     AILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIAPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIVIAFDLFS"
     gene            complement(5498..6277)
                     /locus_tag="VH663_000791"
     CDS             complement(5498..6277)
                     /locus_tag="VH663_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEQ8174008.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETLLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYIAQ"
     gene            complement(6310..6969)
                     /locus_tag="VH663_000792"
     CDS             complement(6310..6969)
                     /locus_tag="VH663_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ8174009.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(6971..7622)
                     /locus_tag="VH663_000793"
                     /pseudo
     CDS             complement(6971..7622)
                     /locus_tag="VH663_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
     gene            complement(7646..8833)
                     /locus_tag="VH663_000794"
     CDS             complement(7646..8833)
                     /locus_tag="VH663_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ8174010.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQVAVHSGIPEDKTAFTLDMVCGSGLKAIQLAAQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALGNHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGTGPIPATQKALKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            9032..9928
                     /locus_tag="VH663_000795"
     CDS             9032..9928
                     /locus_tag="VH663_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ8174011.1"
                     /translation="MDIKQIRYFIAIVENHFNLNQAAELLYVSQPTLSMMINDFEKRE
                     NVKLFKRKRGRIIGLTYLGDNYYKDAQKVLSLYDDMFLKLHDHSKGLKGSINIGIPPL
                     ILSVVFSEVMPKLILENPGIQFNLKEIGAYQLKNELLVGNVDVAVLLSPTGIADNLVE
                     TYEIQRSELSVCLSPRHRLASKKVIQWEDLTDEQLALFDPSFMVHHLVLEACERHQVR
                     PNIILTSSSWDFMLNSTKINHNVLTICPKPITELYQFKDIKCIPMERPISWRVVLTRL
                     RKKSYSEIEAYIMDDLLQSFFK"
     gene            10058..11665
                     /locus_tag="VH663_000796"
     CDS             10058..11665
                     /locus_tag="VH663_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEQ8174012.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            11775..12410
                     /gene="gmk"
                     /locus_tag="VH663_000797"
     CDS             11775..12410
                     /gene="gmk"
                     /locus_tag="VH663_000797"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEQ8174013.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKKTFKAK"
     gene            12426..12743
                     /locus_tag="VH663_000798"
     CDS             12426..12743
                     /locus_tag="VH663_000798"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEQ8174014.1"
                     /translation="MMLKPSIDTLLDKVSSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            12808..15192
                     /locus_tag="VH663_000799"
     CDS             12808..15192
                     /locus_tag="VH663_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEQ8174015.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKAIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERQIDRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEAKILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            15254..16189
                     /locus_tag="VH663_000800"
     CDS             15254..16189
                     /locus_tag="VH663_000800"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEQ8174016.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTILLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSD
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGA
                     LSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR"
     gene            16179..17501
                     /gene="rsmB"
                     /locus_tag="VH663_000801"
     CDS             16179..17501
                     /gene="rsmB"
                     /locus_tag="VH663_000801"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEQ8174017.1"
                     /translation="MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLTYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSDRAVAIMESLFVRSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            17539..18279
                     /locus_tag="VH663_000802"
     CDS             17539..18279
                     /locus_tag="VH663_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEQ8174018.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            18276..20174
                     /gene="pknB"
                     /locus_tag="VH663_000803"
     CDS             18276..20174
                     /gene="pknB"
                     /locus_tag="VH663_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEQ8174019.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSREHKIIFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGELSLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSP
                     SETTAQTP"
     gene            20297..20989
                     /locus_tag="VH663_000804"
     CDS             20297..20989
                     /locus_tag="VH663_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEQ8174020.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            20986..21990
                     /locus_tag="VH663_000805"
     CDS             20986..21990
                     /locus_tag="VH663_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8174021.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLHMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            21983..22624
                     /locus_tag="VH663_000806"
     CDS             21983..22624
                     /locus_tag="VH663_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8174022.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            22661..24061
                     /locus_tag="VH663_000807"
     CDS             22661..24061
                     /locus_tag="VH663_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ8174023.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPRLQEKADVITKKVEEDGILYALEELGLIDKELQFPQL
                     DLANHKGPKATIKTNHGDMTLVLFPEHAPKTVANFLGLAKEGYYDGIIFHRIIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQYFIVQNSKVP
                     YAKKELERGGWPAPIAASYAENGGTPHLDRRHTVFGQLVNETSFQVLDLIAGVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            24061..24438
                     /locus_tag="VH663_000808"
     CDS             24061..24438
                     /locus_tag="VH663_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ8174024.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALDNGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKASLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(24456..25397)
                     /gene="cysK"
                     /locus_tag="VH663_000809"
     CDS             complement(24456..25397)
                     /gene="cysK"
                     /locus_tag="VH663_000809"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEQ8174025.1"
                     /translation="MTKIYKTITELVGQTPIIKLNRLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKASPETAIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARRTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIETK"
     gene            complement(25525..26157)
                     /locus_tag="VH663_000810"
     CDS             complement(25525..26157)
                     /locus_tag="VH663_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEQ8174026.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNSQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSSMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            26212..27537
                     /locus_tag="VH663_000811"
     CDS             26212..27537
                     /locus_tag="VH663_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8174027.1"
                     /translation="MESIENSYGRLLLESQLPDSARQLAQPLKSVVISRGKMICQRCH
                     YQLDEEARLPSGAYYCRFCLVFGRNQSDKLIYAIPPMHFPKGNYLVWSGQLTAYQEMI
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFN
                     LFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACVS
                     SQSQNRKEGITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAG
                     RVGRSIDRPDGTLYFFHEGISKAMLLAKKEIKEMNYKGYSHDLSTMSTN"
     gene            27509..28174
                     /locus_tag="VH663_000812"
     CDS             27509..28174
                     /locus_tag="VH663_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEQ8174028.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACQDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSLGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            28254..28802
                     /gene="raiA"
                     /locus_tag="VH663_000813"
     CDS             28254..28802
                     /gene="raiA"
                     /locus_tag="VH663_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEQ8174029.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDDATNILYRREDGNLGLIEAK"
BASE COUNT         9395 a         5180 c         5930 g         8566 t
ORIGIN      
        1 ttatagtcct aaagatattt ttgagcaaga atttaagact agtatgcgag ggtttgataa
       61 gaaagaagtt gatgaatttc ttgataatgt aattaaagat tatgaaaatt tcaatgctca
      121 aattgaagct ttaaaagctg agaacgaagc cttaaaaaag gcgaagtttc aagctagaaa
      181 tacagtttca gcgactgttc aacaaccagt accgcaaccg actcgtattg ctcaatcagc
      241 tactaatttt gacatcttga aacgtattag taaattggaa aaagaggttt ttggtaaaca
      301 aattattgag taatgaaaac tgtgcaattt ttggataatc gcgtagtatt ttaatactat
      361 gaggaaagtc catgctagca ctggctgtga tgccagtagt gattgtgcta ggcgaacaca
      421 taagcctagg gatgtgcata cacattacgg cgaaggaaat ggctaagtcg tttgatatgc
      481 caaagtactt ctgaaagtgc cacagtgacg tagtttttat ggaaacgtaa aaaatggaac
      541 gcggtaaacc cctcaagcta gcaacccaaa ctttggtagg ggcatgggat agttggaaac
      601 gaacaagcta tcctgactgt caacagacgg tagacagatg attatcgaag gaaataattc
      661 ctagttattt ccggaacaaa acatggctta tagaaaattg cataataggt taagctagca
      721 cttgctagct tttatttgat ttaaaagaaa gagatatatg aaagaaacat ttagattagt
      781 cgcaactgca gccgcaggtt tagaagctgt cgttggtaaa gaagtcagag cactaggctt
      841 tgattgtcag gttgaaaatg ggaaagttta ttttgaaggt gatgtagaag cgattgttaa
      901 aacaaatctt tggttacgtg cagcagatcg tatcaaaatt atcgtaggac aatttcctgc
      961 tagaaccttt gaagaattat tccaaggtgt ctttgccttg gattgggaaa attatttacc
     1021 gctaggagct aaatttccaa tctcaaaagc caaatgtgtt aagtctaaat tgcataacga
     1081 gccaagtgtt caggcgatta ccaaaaaggc cgttgttaag aaactgcaaa agcatttcca
     1141 caggccagaa ggagttcctc ttcaagaagt tgggtcaacg tttaacattg aggtatctat
     1201 tttaaaagac caagccacta ttatgattga cacgacagga tctagcctat ttaaacgagg
     1261 gtatcgcgtt caaaaaggtg gagcaccgat caaggaaaat atggcagctg ctatcttggc
     1321 cttatctaac tggtttccag ataaacctct ggttgatccc acctgtggat cgggaacgtt
     1381 ttgcatcgag gcagccctga ttgggatgaa tattgctcca ggatttaacc gttcgtttgc
     1441 ttttgaagaa tggagctggg tagataaaga catggtgcaa caagttcgtg atgatgctga
     1501 acaagaagct aattatgaaa tcgaattaga tatttctggc tttgatatcg atggccgcat
     1561 gattgagatt gctaaaagta atgctgaaga agctggttta tctgatgtga ttacctttaa
     1621 gcaaatgcgt ctgcaagact ttcgaacaga caagattaat ggtgtagtga tatcaaatcc
     1681 tccttatgga gaacggttgc ttgatgacaa agcggttgac atcttgtata atgaaatggg
     1741 acaaaccttt gcaccattga aaacatggag caaatttatc cttacaagtg atgaactttt
     1801 tgaactaaaa tacgggcaaa aagctgataa aaaacgtaaa ctttataacg gcaccctaaa
     1861 agttgatttg taccaatttt atggggaaag ggtaaagcga catctgatat agagaggtag
     1921 taagcgtgtc agaagaaagc aaagaagtcg aagtaacaaa agaatcgcaa acattggggc
     1981 ttaatgaagc caagtcaatg acaattggtg aagctgtacg caaacagtca gagataaaag
     2041 caggtgttac taaggatgat agtattttag ataagtacat taagcaacac cgtgacgaag
     2101 tttcttctca aaagtttgat gctaagtata cagagcttga tacagctagt ttagataact
     2161 ttatcaaaaa acaaagagaa gcattgagta aagccggtct tgttgatgac gaacctgtgt
     2221 ctgcggagtc tgctgagcaa gatagcactt tggttgaaga agtagctgaa gacttagctc
     2281 caatggagac tactgcggtg gtaactggga ttccggtgga agcgacagtc cctgtgttag
     2341 atctagatcc atcagagagg gtcattccgg aaccacagat aaccaaagaa gagccaaaaa
     2401 gagatcagtt tctttctgaa gatagccacc atccagccaa acaaaacacg aaaaaaggtt
     2461 ggttaatagc tttatttctg ctattattgg ccattcttgc agttgtattt ggttggaacc
     2521 atttcttgag acaagacagt ggtaaaacaa ctcaaacagc aagtaaacag acaaagacct
     2581 ctttgcagac cgacagtgct aaaaaagcaa cgcgcttgaa ggctgctgct aaagctttcg
     2641 aaaaacaata tggtactttt tatacagatg ccacaaagag caaattaaaa aatagtgcct
     2701 ttgctacttt gccagattta gaagctgcct tgaaagcttt ggaaggctct gcttactatg
     2761 ataaggcgaa agcaaaagtc gattccctta agaaagctat tgctgctatt acagcggtta
     2821 acggtaaatt tgtttccgat gttgttgtag atggtgaaaa ggtttcggct gaagtcaagg
     2881 ctgatgctaa ttttgatgat ttatcaagtg caaccttgac aacaggaaat gcaaatcttg
     2941 atgctgtctt gcaggcaagc atcacagaag gtcgtcagca gctagccagc aaggctgagg
     3001 ccgctaaagc agctaatgag caagcagccc aggctcaagc agctcaagga caaagtacat
     3061 cagtagcacc atctggctat ggcttgacaa gttatgaccc tgcaagctta caacggcatt
     3121 tatcacgtgt accttacaat caagatgtta ttgctgatag agctaaccca tcttgggcat
     3181 ttaatccagg agttcttgaa aaaattgtag caacctcaca agctagaggt tatatttcag
     3241 gcaaccaata tattttagag ccggttaata ttattaatgg caatggttac tataatatgt
     3301 ttaagcctga tggcacttat ttgttctcta ttaattgtaa gacgggttat tttgtaggaa
     3361 atggcaaagg ttatgctgat gctttagatt actgagaaaa tcaaaaaaac tgagataagg
     3421 caacttgtct cagttttttg attaataagg tatgttgtct taaaatctgc agatagactt
     3481 gttttttgta acaagtaaaa taggtcaata ggattgttat gtttgacaac acactagtat
     3541 cagatgacat ggcgactaaa aggatcgtct gaaatccctt gatcaataat caattgagcc
     3601 cattctttgg cggcaaagag gctatgatcc ttatagttcc cacaggattc aagagttgtt
     3661 ccaggaacat cttcccaagt agtcccagtt gcaatttctt ctaggctgga tttaatcacc
     3721 ttggcaatat cagtagaact gtgttttccc cacatgataa ggtgaaaacc tgttcgacag
     3781 ccaaaaggag agcaatcaat catcccatca atgcgttggc ggatgagctt ggcaagtaag
     3841 tgttcaatgg tatgcaaacc ggctgtttca atagaatttt ggttaggctg cactaggcga
     3901 acatcaaaat ttgtaatacg atcgcccttg ggtccaaatt cttcagaaat aagacgaaca
     3961 taaggggcct ttacaatagt atgatctagc tcaaaacttt cgacgataac ttcttttgtc
     4021 atttcaatct cctttgtttt acactttatc ataacacaaa tagtgagaaa gtggctactt
     4081 gtggaggaga aaaggctaag aaaacagatc aaaggctatc acaatgacct gtgcgataaa
     4141 agtacttaca gaaacagtaa taaaagtttc tttaaaagca tttttgtggt taagtcctgt
     4201 caatgctaag aaggtgagaa agacaccact ttggggcaca atggtaaaga tattcgaagc
     4261 aatagtggca acacggtgaa tcatttcggg attaaggcct tgatctaaat aatactgggc
     4321 aaaattaggc atgacaattc ctagagcacc tgaagaagaa ccggtaatag ctgacataga
     4381 ggaagtcaac acggctaaac taataagagg attaccggga atattcaaaa tgagatctga
     4441 gaagaatgtg aaacctgggg caatcatcac aacggctccg aaggcaactg cagatgctgt
     4501 cgcaaaaata ggtgcaatag agctgctagc tcctaaatta aagacagcaa tcttattagg
     4561 aataaactgt ctaaagagcc agcttgcggt gagaatggca gctaaaagtg ctatgaaaat
     4621 aatgttcttc ttaaaaaaat cgtttccgaa aaggctaccg gttagggcga taataatgag
     4681 caacaataaa ggtaagatac tggcaaggaa atgtgggaga ttttttgttt tcacttgtat
     4741 ctcattgtca aaagcataag tggcataagt ttcacccatt gccatacttt ttgctagaca
     4801 atatttcata taaaagagcc caaaagtcac gcagccgatt gagccaacga tactcggaat
     4861 ggcggcagca gttagtgagg tgtccaagta ttgaatagga ataacatttt gaatggcagg
     4921 tgttccgggt aaaatcgtca tggtaaaagt cgcaataccg agccaaagag gaacttgaat
     4981 taagttccaa gccaagtcca tttttttgaa tagacttcgt gcaagcggca agacagcaaa
     5041 catgacaaca aagaggctga ttcccccgta agttaatata gcactgatta gaaaaatagc
     5101 aacgagtact ttataaggac tatcatgacc aacttttttt aaaatataat ctgcaataga
     5161 agtggttgca ccgcttgttt ccattaattt ggctaagata gaacctaata aaaagatagc
     5221 aaaataattg agaatataag tggagagtgc ccccataaat tggttgggtt cttttccaag
     5281 taaggttttg gttggatcca tttgattaaa caaaatgacc aagctggtag caagaggcgc
     5341 agcgatgata atgttaactt ctttgacaga caggtaaata ataacaataa cacctaccaa
     5401 gacccccaga gaaccaataa tttctatagt tttatgacct ccttattaga aaataaagag
     5461 acagatcaca agatacagcc tctttatctc tccaaattta ttgagcaatg tagccaccgt
     5521 ctaagataca ggcttgacct gtaacacctt ttgccttatc actggcaagg aaagacacat
     5581 agtctgcaat ttcttgaacg tcaatgaggc gtttttgagg aacaagtggg tatagcactt
     5641 cttcaagaac attttcaagg ggaatacctc ttgttttgga aaggtcttca aactggccac
     5701 gaaccagtgg tgtgtcaaca tatccaggac aaatggcatt gaccgtaatg cctgaatcag
     5761 cagcttctaa ggcagttact ttggtcagac cgatcaagcc gtgcttagct gaattgtagg
     5821 cggatttgcc agcaaaacca atgacaccat tgatagaagc catattaata atacgaccgt
     5881 gtttttgagc tttcattgta ggaaaagcac gtttaatggc aataaatggt gctgtcaaca
     5941 tgattttaat catgaattca aatttttcag ttggaaaatc ttcaatcatg gcaacatgtt
     6001 gaaggcctgc gttgttaacc aaaatatcaa tacgaccata tttttcaaca accgtatcaa
     6061 tagcagcatt gatggcttct tctttggtga catcgcacac aacactaaaa gcgtcatagc
     6121 cttctttagt atagtcagca acaacctgat ccagtttttc ttgattaatg tcggaaaaaa
     6181 caaccgtttt accttctttt aataaggttt ccccgatttg ttttccaatt ccgcttgcag
     6241 caccagtgat aaatgcaact tctttagtat gtgtcatgat actatcctcg tctttctttt
     6301 ggttagtctt tagtttgttt tatcaattag ggtaacttcg gtaacgtttt gcacgtcttc
     6361 aaaggtgtaa tctgggttaa tttcaagggc gcatagacct tcatcttgat attcaaagac
     6421 tcccatttca gtaatgatta ggttaacaac attttgagca gtcagaggaa gggtgcattt
     6481 gtctaaaatt tttgctttgc ctttattggt atgctccatt gcaacaatga cttttttagc
     6541 acctaccaat aagtccatgg cccctcccat cccaggaacc atttttccag gaatcaggta
     6601 attagcaatg ctagcttctt tatcaacttg taaggcacct aagacggttg cggccacatg
     6661 gccgcctcga ataataccaa aggaagttga gctatcgaag aaagcccctc caggagcaat
     6721 actaacaggt tgtccaccag cattaacaat actaggatcg tagtgatcat ctgttaatcc
     6781 agtgagaccg acaaaaccat tttcggactg cagggtaatg gttacacctt caggaatata
     6841 gttggctact tttgttggca aacctatgcc taagttgacc agtgtattgt gttctaattc
     6901 ttgtgcaacg cgcttagcaa tacgtgtttg aatctcttct tttgaaagaa cagtagctga
     6961 tgttcccatg ttaggctcct ttcactaagt agttaacaaa aatgccagga gtatggacaa
     7021 aattaggatc catttgcccc acgtcaacaa tttctcttgc ttcaacaatg gtggttttcg
     7081 cattagcagc catcacatga ttaaagttat tttcagaacc tgcgtattgc aaattgccat
     7141 ttttatctgc cttgttagca aaaataagtg cgatatcagc tttcaaaggt ttttcaagaa
     7201 ggtagtcttt gccgtcaata gtaatgactt cttttccttt ggcaacttcg gttcctattc
     7261 cagtaggtgt taagaaaccg cctagcccaa aaccgccact gcggatgcgc tcagctaatg
     7321 ttccctgagg tacaagatca attacagtct cgccctcagt catttgtcgg cccgcttcac
     7381 ggttgagacc gatatgtgat gcaatatata gttgaaaact gtttattggc aaccatctta
     7441 ccaacgcctt tgtcaggaaa aacagcatcg ttacagatta aagtgaggtc tttgacacct
     7501 ttttccacaa gtgcatcaat taatttttct ggtgtgccat ttgtcatgaa gccaccaacc
     7561 ataatggtat caccatcttt aacgtgagct accgcttctt ttattatgat ttctttacct
     7621 attttttcta ccaactttca cgagactaag tattgttttt cacgataaca gcagttcctt
     7681 gtcctccacc aatgcaaagt gttgctagtc ctctggttac ttggcgtttt ttcatttcgt
     7741 ggattaatgt gactagaatg cgagcgccac tggcaccaat aggatgacct aaagcgatgg
     7801 ctcctccatt aacattcacg atttctgggt ttaactttaa atcttttaaa accgccaagg
     7861 actgtgctgc aaatgcttca ttggactcca ctaagtccaa atcagaaatt gttaatccag
     7921 ctttcttaag ggctttttga gtggcaggaa ttggtccagt tcccatcagt tcaggtgcaa
     7981 cacctgcact agcatagctt tcaatagtga ctaaaggtgt taaccctaat tcaagagctt
     8041 tttcttcagt cattaacatc aataaggctg caccatcgtt tattcctgag gcattcccag
     8101 cagtaacggt tccttcttta gggaggaaag caggacgtaa ttgctgtaat ttttcaaggc
     8161 ttgtgtcaac ttttgggtat tcatccgtag taacaattaa aggatctttg cgtcgttgag
     8221 gcacagagac agggacaatt tcgtcagcaa aacgatgatt tcctaatgcc ttagcagctt
     8281 tttcttggct ttctaaagca aattgatctt gctcttctct tgagatgcca tatttctgag
     8341 caacgttttc agcagtaata cccatgtggt aattattaaa agcatctgtt aagccatcat
     8401 gaacaagagt atcaatcaat tgactattac caagacgttg accaaagcgg tgctgagttg
     8461 agacataagc tgcttgtgac atgttttcca caccaccagc aataacgata tcagcgtcac
     8521 caagcatgat gctttgcgca gctaattgga ttgctttaag cccagaaccg caaaccatat
     8581 ctaatgtgaa agctgtttta tcttcaggaa ttcctgagtg gactgcaact tgtcttgcca
     8641 cattttgtcc taggcctgca tgaaggacgt ttcctaggat gacttcatca accatttcag
     8701 gctttacttg cgttttatca aggatttttt tcagtacagt tgttccaagt tcaacagcag
     8761 agacactttt aaaggagcct ccaaaagctc cgataggagt tcgtaaagct tcaacgataa
     8821 ctatgttttt cataaaataa acatcctttc agttttagaa agcgttttct atatctctaa
     8881 gataacaaga aaccgctcaa aataaaaata tagaattttt ataagcatat aaattttttt
     8941 gatagatttg gcaatagcaa ttaaaaatca cttatgtcat aatggaatta atactatcgg
     9001 aaaaaagact aatagacaag gggataacca tttggatatt aaacaaattc gttattttat
     9061 cgcaatcgtt gaaaatcatt ttaacttgaa ccaggcggcg gaactgttgt atgtgtcgca
     9121 gccgacactt agcatgatga tcaatgattt tgaaaaaagg gaaaatgtta agctcttcaa
     9181 acgaaaaaga ggtcgtatta ttgggttaac ctatcttggt gataattatt ataaggacgc
     9241 tcaaaaggtt ctcagtctct atgatgacat gtttttgaaa ttgcatgatc acagtaaagg
     9301 gttaaaggga agtatcaata ttggtattcc tcctcttatt ttatcagttg ttttctcaga
     9361 agtaatgcca aaactgattt tagaaaatcc gggaattcaa ttcaatctta aggagattgg
     9421 tgcttaccaa ctaaaaaatg aactactcgt gggaaatgtt gacgtcgcag tcttgttatc
     9481 cccaacaggc atcgctgata atttggttga gacttatgaa atccagagat cagaattatc
     9541 tgtatgcctg tctccacgcc atcgtttagc ttctaaaaaa gttattcagt gggaagattt
     9601 aacagatgaa caacttgccc tatttgatcc tagtttcatg gttcaccatt tagtgcttga
     9661 agcttgcgag cgtcaccaag tcagacctaa tattattttg acgtcctctt cctgggattt
     9721 tatgcttaat tctaccaaga ttaatcacaa cgtcttaact atttgtccca aacctattac
     9781 agaattatac caattcaagg acattaagtg tattccaatg gaacgtccca tttcttggcg
     9841 tgttgtctta acacgcctcc ggaaaaaaag ttattctgaa atagaagcct acatcatgga
     9901 cgacttgcta cagtcttttt ttaagtaggg ttctcaacta cttggcccga atgttaattt
     9961 ttttaaaaat gaattggaat tgtgtaaacg ctcaatttat gataaactaa gagatggctt
    10021 tctattaatg aaatctagaa tatattgagg tacaaacatg gttaatatta ttttattaat
    10081 tgtttctgcc ctcattggtt taatattagg ttatgcactt atttcgatta gactcaagtc
    10141 tgcgaaggaa gctgcagagt tgactctttt aaacgctgaa caagaagctg ttgatattcg
    10201 tggcaaagca gaagtagatg ctgaacacat caaaaaaaca gctaaacgtg aaagtaaagc
    10261 aaatcgtaaa gaattacttt tagaagcaaa agaagaggca agaaaatatc gtgaagagat
    10321 tgaacaagaa tttaagtctg aaagacaaga gcttaaacaa ctcgagacac gcttagcgga
    10381 gcgctcctta actcttgacc gtaaagatga aaacctatca agtaaagaaa aggtactaga
    10441 tagtaaagaa caaagtctga ccgataaatc taaacacatt gatgagcggc aacttcaagt
    10501 ggaaaaactt gaagaggaga aaaaagcaga actggaaaaa gttgctgcga tgacgattgc
    10561 agaagcgcgt gaagtgattt taatggagac ggaaaacaaa ctgacccatg aaattgcgac
    10621 gcgcattcga gatgccgaac gtgacatcaa ggaccgaaca gttaaaacag ccaaggactt
    10681 gttagcgcaa gccatgcaac gccttgctgg tgagtatgtg actgaacaaa ctattaccag
    10741 tgtccatctc ccagacgaca acatgaaggg ccgaattatt ggacgtgaag gccgtaatat
    10801 tcgtacttta gagagcttga ctggcattga cgttattatt gacgatactc ctgaagttgt
    10861 tatcttatca ggatttgatc ctattcgacg tgaaattgct cgtatgacct tggaatctct
    10921 gattgctgat ggtcgcatcc atccagctcg tatcgaggaa ttggttgaga aaaatcgtct
    10981 tgaaatggat aatcgtattc gtgagtacgg tgaagctgca gcctatgaga ttggtgcacc
    11041 aaaccttcat cctgatttga ttaaaatcat gggacgcctg caattccgta cctcgtttgg
    11101 tcaaaatgtc ctacgtcact ctgttgaggt tggtaagtta gctggtattt tagctggtga
    11161 gttaggtgaa aatgttgctc ttgcccgccg tgctggtttc ttgcacgata tgggtaaagc
    11221 tattgaccgt gaggttgaag gtagtcacgt tgagattgga atggaatttg cacgtaaata
    11281 caaagaacat ccagttgttg tcaacactat tgctagccac cacggagatg tggagccaga
    11341 ttctgtgatc gctgtgctag tagctgcagc agacgctctc agttcggctc gtccaggcgc
    11401 tcgtaatgag tcaatggaga attacatcaa gcgtcttcgt gatttagaag aaatcgcgac
    11461 aagttttgat ggggtacaaa atagttttgc gctacaagct ggacgtgaga ttcgtattat
    11521 ggttcaacct gaaaaaattt cagatgatca ggttgtcatt ttgtcgcata aagtaagaga
    11581 aaaaattgaa aacaatctag attacccagg aaatattaaa gtaactgtta ttcgtgagat
    11641 gagagcggtt gattatgcca agtagaaaaa gaggaattat cctctttttc tttatgactt
    11701 gcctttgaaa atgttcttga aaaagactag cctttttgat aaactattaa aaagcaacta
    11761 ataaggagaa tacaatgtct gaacgcggtt tgttaatcgt cttttctggc ccttcaggtg
    11821 tcggaaaagg aacggtaaga caagaaattt tttcaacgcc agatcataaa tttgaatatt
    11881 cggtttcaat gacgactcgt ccgcaaagac cgggcgaggt tgatggggtt gattatttct
    11941 tccgcacacg tgaagaattt gaagaactca ttaaaactgg tcaaatgcta gagtatgctg
    12001 aatatgttgg taattactat ggcacccctt taacttatgt taatgagact cttgataagg
    12061 gaattgatgt ctttttagaa attgaagttc aaggagctct tcaggttaag tcaaaagtac
    12121 cagatggtgt ttttgttttt ttaacaccac cagatttaga tgaattagaa gaccgcctgg
    12181 ttggtcgtgg tacagatagt caagaagtca tagctcaacg tattgaaaga gcaaaagaag
    12241 aaattgccct tatgcgcgaa tacgattatg cagttgttaa tgatgaagtt gccttggcag
    12301 ctgagcgggt taagcgtatc attgagacag aacactttcg tgtggagcgc gtgattggtc
    12361 gttatgataa aatgattaaa ataacaaaaa aaacctttaa agctaaatag aaaagagtaa
    12421 gcattatgat gttaaaacct tccattgata ccttattaga taaagtatca tccaagtatt
    12481 cacttgtgat tttacaagcc aaacgtgccc atgaactaga agctggtgcc actccaactc
    12541 aagaatttaa atcagtgaag tcaacacttc aggcacttga agaaattgaa tcaggaaacg
    12601 ttgtgattca cccagatcct tcagctaaac gtgaagctgt ccgtgctaag attgaggcag
    12661 aacgcttggc caaagaggaa gaggaacgta aaatcaaaga acaaattgct aaagaaaaag
    12721 aagaagaagg agaaaaaatc taaggtttca gaaatcttag attttttcat atttagtacg
    12781 atgtccatga cagaaaagga gaagcaaatg gaaaaagtag ctcatgtgat tgtagatatt
    12841 ccattgatgc aaacggacaa gccattttct tatggtattc caaaagagtt agtttctctc
    12901 gtgcagcttg gttctcgtgt gcatgttcct tttggaaaag ggaatcgttt gttgcaaggc
    12961 tttattattg gttttggcca agaagattca agctctctta aactgattca gaccgtttta
    13021 gatcctgagc cagtcctaaa ccaagaacaa ttgacccttg ctgatcagct tcgaaaaaca
    13081 gttttttctt ataaaatcac gctcttaaaa gcaatgattc ctaatcttct taactctaac
    13141 tatgataaag tgctaaggcc agagtcaggt ctaaaaaagc ctgatagaga ctttcttttt
    13201 gaaggaaaac catctgtctt atattctacc ttagaccgcg agaaggaaaa aatagctctt
    13261 aaggccatac aagctgggca tattactgtt agttatttag ccaaagacaa gaaaaactta
    13321 aagacagaaa aatattatca cgtcgactta gacgccttag cggtacatcc catctctagc
    13381 cgtgccaaaa agcgtcaatt attaaaagac tatctgctaa cccatacgaa ggaagctaaa
    13441 cttgcaacgc tttatcaagc cttttctaga gatgtggtgg cttactttgt gacaaatcac
    13501 ttaattcgta ttgacgagcg tcaaattgat cgcagtgagc cctattttga ccagatcaaa
    13561 ccatcttctt ttttaacctt aaatgaacag caagcgtcag ctgttactga aattgttgag
    13621 caaattggga aaccgagtaa accttttttg attgaaggga ttacaggttc gggcaagaca
    13681 gaagtttatc ttcatatcat cgaagcggtt ttaaaacagg ataagacagc cattgtttta
    13741 gtaccagaaa tttcgttgac tcctcagatg acctctcgtt ttatttcccg tttcggaaag
    13801 caagtggcta ttatgcattc aggcttatca gatggtgaaa agtttgatga gtggcgcaag
    13861 ataaaaacag gacaggctaa ggttgtggtt ggtgcccgct ctgctatttt ctccccacta
    13921 gagcggattg gtgcaattat tattgatgag gagcatgaat caacctataa acaagaaagc
    13981 aaccctagat accatgccag agaggttgcc ctgctcagag ctaaacacca tcaagcagtt
    14041 gtggttatgg gctctgctac gccaagtatt gaaagccgag caagggctag taagggtgtc
    14101 tatcatttta ttcaattaac gcaaagagca aaccctcttg ctaagattcc agaagtaacg
    14161 attgttgatt ttcgtgatta tattggacag caagcagtta gtaattttac cccatatttg
    14221 attgataaga ttaaagaacg tttggttaaa aaagaacaag ttgtcttaat gttaaataga
    14281 cgtggctatt ctagttttgt gatgtgtcgg gactgtggtt atgtggacaa atgtcccaac
    14341 tgtgatatct ctttaaccct tcacatggat actaaaacta tgaattgcca ttactgtggg
    14401 tttcaaaaac ctatcccaat tacttgtcct gagtgtcata gcaatagtat ccgctattat
    14461 ggcactggaa ctcaaaaagc ttttgatgaa ttgcaagggg ttattccaga agctaagatc
    14521 ttgagaatgg atgttgacac cacacgaaaa aaaagaagtc acaaaaccat actagatagt
    14581 tttggtcgcc aggaagcaga tattttgtta gggacgcaaa tgattgccaa aggactggat
    14641 tttccaaatg tgactttagt tggggtacta aatgcagata cctccttaaa tttgccggat
    14701 tttcgagcgt cagaaaaaac gtttcaattg ttaacacagg ttgctggtcg ggcaggccgt
    14761 gcccataagc caggagaagt cttgattcaa acttataatc ccgatcacta tgctattcaa
    14821 ttagctaaga aacaagattt tgaagccttt taccgttatg aaatggcgat tcgccgtcag
    14881 atggcttatc ccccttacta ctttacagtg ggtattacgc tttcgcatcg tttagaagca
    14941 tcggttgtaa aaaaagctta tcaagttaca gaactgctaa agagtcactt atcggataat
    15001 attaaaatat taggaccgac tcctaagcca attgctagaa cacacaattt ataccactat
    15061 caaattttgt taaaataccg ttttgaagat aacctagaag agacgctcaa tcgtattctt
    15121 gattggagtc aagaagcaga taatcgtcat ttaaaactaa ttattgattg tgaaccgcaa
    15181 caatttttat aaggtaaatc actaaatcta ggatgatcag ttatgatata ataaaaatac
    15241 aaagaggaaa gtcatgataa aattattatt tatgggaact ccccaatttt cagcaactgt
    15301 attgaagggc ttgctagata atcctgccta tgagatttta ggagtggtta ctcagcctga
    15361 tcgagctgtt ggacgcaaaa aagatattaa ggtaacacct gtaaagcaat tagctcttga
    15421 gcatggaata tcaatctatc agccggaaaa attatcaggc tctcaagaac tcattgagat
    15481 tatgggactt ggtgcagatg gtattattac agcagcattt ggccaatttt taccaacaat
    15541 attacttgat tcagtttcat ttgctattaa tgtacatgca tcattgttac ctaaatatcg
    15601 aggtggagca cctatccatt atgctattat gaatggagat aaagaggccg gcgttactat
    15661 tatggaaatg attaaagaaa tggatgcagg tgacatggtc gctaaagcaa gcactccaat
    15721 tcttgaaacc gataatgtag gtactctttt tgagaaatta gccatcattg gtcgagactt
    15781 acttttggat agtttgccag cttatctttc tggagaatta aaacctattc ctcaagatca
    15841 cagccaagct actttttcac ctaatatctc tccagaacaa gaaaaattag attggacaat
    15901 gtctgatcaa gaagtgttca atcatattcg aggtatgaac ccttggccgg tagcgcatac
    15961 gtttttagag gggcaacgtt taaaaattta cgaagctcaa ctagctgaag gagagggatt
    16021 acctggacaa gttattgtca aaaccaaaaa atctttggta atagctactg gacaaggtgc
    16081 tttgtccttg atcgttgtgc aaccggctgg taaacctaaa atgtcaataa tagacttttt
    16141 aaacggcatt ggtagaaaac ttgaagtagg ggacatcatt ggcagataat tggaaaaaaa
    16201 gtacgcgagg caaagccctc cttgtcattg aagctatttt tgatcagggg gcttacacaa
    16261 acatagcgct taatcagcaa ctaagcaata aagctttatc tgctaaggac agagctctat
    16321 taactgagat tgtatatggt accgtttcac gtaaaattag cttggaatgg tatttagccc
    16381 attatgtcaa agaccgagat aaattggaca aatgggttta ttatctcctt atgctcagtc
    16441 tttatcaact gacttattta gataagttac cagcgcatgc cattgttaac gatgcggttg
    16501 gtattgccaa aaatcgtggc aataaaaaag gtgcagagaa gtttgtcaat gctattttac
    16561 gtcaattcac cagccatcct ctcccagata tggaaacaat taagcgtcga aataagtatt
    16621 attcggtcaa gtattcactg cctgtttggt tggttaaaaa gttagaagac caatttggct
    16681 ccgacagagc agtggctatt atggagagtt tatttgtgcg tagtaaggct agtattcgcg
    16741 tgacggaccc tttgaagtta gaagaagttg ctgaggcttt agatgcagaa cgttcccttt
    16801 tatcagcaac tggtttaacc aaagcgtcag gtcattttgc agccagtgat tatttcacaa
    16861 atggggacat cactattcaa gatgaaagca gtcagcttgt agctcctact ttaaacattg
    16921 acggtgacga cataattctt gatgcgtgta gcgctccagg gggcaagacc tctcatatag
    16981 catcttactt aaaaacaggt aaagttattg ccttagatct ctatgatcat aaactagagt
    17041 tggtgaagga aaatgctaac cgcttaggag ttgctgataa cattgagaca agaaaactag
    17101 atgccagaga ggttcatcgt cattttgaaa aggatagctt tgataagatt ttggtggatg
    17161 ctccttgctc aggaattggt ttgattcgtc gaaaacccga tatcaaatat aataaagaaa
    17221 gtcaagattt taatgctcta caggctatcc agctagagat attgtctagt gtttgtcaaa
    17281 ccttgcgtaa aggtggtata ataacataca gtacttgcac tatttttgat gaggagaatc
    17341 gtcaagtcat cgaagcattt ttacagagtc atcctaattt tgaacaagta aaactgaacc
    17401 atacacaagc agatattgtc aaggatggtt atcttattat cacaccagaa caataccaga
    17461 ctgatggctt ctttattgga caagtcagac gagtcttgta gtattttagg aggaaactga
    17521 ctgagtcaga aaaaacgtat gaagatttca ttaaaaacag acattgggca aaaacgctca
    17581 aacaatcaag attttatcaa taagtttgat aataaaaaag gcatcacttt agtgatttta
    17641 gcagacggta tgggtggcca cagagctgga aatattgcca gtgagatgac agtcacagac
    17701 ctgggaagag aatgggtaaa aacagacttc acagagttaa gtcagattag agattggtta
    17761 tttgaaacta tccagtcaga aaatcaacgg atttatgatc taggccaatc agaagacttt
    17821 aaaggaatgg gaacgactgt tgaagcagta gctttagttg aatctagcgc catttatgct
    17881 catattggtg attcacggat tggtcttgtc catgatgggc attatacctt attgaccagt
    17941 gatcattctt tagtgaatga gttggttaag gcaggacaga tcacagaaga agaagctgct
    18001 agccatccac aaagaaatat tatcactcaa tctattggtc aagcttcccc agtagagcct
    18061 gatttaggag tgagggtttt ggagccaggg gattatcttg tcattaacag tgatggtcta
    18121 accaatatga tttcaaatga tgaaattgtg actatcttgg gcagtaaggt tagtcttgat
    18181 gaaaaaaacc aagaaatgat tgacttagct aacctaagag gaggcctaga taatattaca
    18241 atcgctttgg ttcacaacga aagtgaggac gtcgaatgat tcagattggc aaattatttg
    18301 ctggtcgtta tcgcattcta aaatctattg gccgcggtgg tatggcggat gtttatttag
    18361 caaatgactt gatcttggat aatgaagacg ttgcaatcaa ggtcttgcgt accaattatc
    18421 aaacagatca ggtagcagtt gcgcgtttcc aacgagaagc gcgagccatg gctgaattga
    18481 accatcccaa tattgttgcc atccgggata taggtgaaga agacggacag caatttttag
    18541 taatggaata tgtggatggt gctgacctaa agagatacat tcaaaatcat gctccattat
    18601 ctaataatga agtggttaga attatggaag aagtcctttc tgctatgact ttagcccacc
    18661 aaaaaggaat tgtacacaga gatttaaaac ctcaaaatat cctactaact aaggagggtg
    18721 ttgtcaaagt aactgatttc ggcatcgcag tagcctttgc agaaacaagc ttgacacaaa
    18781 ctaattcgat gttaggcagt gttcattact tgtctccaga acaggctcgc ggctccaaag
    18841 cgacgattca aagtgatatt tatgcgatgg ggattatgct ctttgagatg ttgacaggcc
    18901 atatccctta tgatggcgat agtgctgtta cgattgcctt gcaacatttt caaaaacctc
    18961 ttccatctat tatcgaggag aaccacaatg tgccacaagc tttggagaat gttgttattc
    19021 gagcaacagc caagaaatta agtgatcgtt acgggtcaac ctttgaaatg agtcgtgact
    19081 taatgacggc gcttagttat aatcgtagtc gggagcataa gattatcttt gaggatgttg
    19141 aaagtaccaa acccctcccc aaagtggcct caggtcccac cgcttctgta aaattgtctc
    19201 cccctacccc aacagtgtta acacaggaaa gtcgattaga tcaaactaat caaacagatg
    19261 ctttacagcc ccccaccaaa aagaaaaaaa gtggtcgttt tttaggtact ttattcaaaa
    19321 ttcttttttc tttctttatt gtaggtgtag cactctttac ttatcttata ctaactaaac
    19381 caacttctgt gaaagtgcct aatgtagcag gcactagtct taaagttgcc aaacaagaac
    19441 tgtatgatgt tgggctaaaa gtgggtaaaa tcaggcaaat tgagagtgat acggttgctg
    19501 agggaaatgt agttagaaca gatcctaaag caggaacagc taagaggcaa ggctcaagca
    19561 ttacgcttta tgtgtcaatt ggaaacaaag gttttgacat ggaaaactac aaaggactag
    19621 attatcaaga agctatgaat agtttgatag aaacttatgg tgttccaaaa tcaaaaatca
    19681 aaattgagcg cattgtaact aatgaatatc ctgaaaatac agtcatcagt caatcgccaa
    19741 gtgcgggtga taaatttaat ccaaacggaa agtctaaaat tacgctcagt gttgctgtta
    19801 gtgatacgat cactatgcct atggtaacag aatatagtta tgcagatgca gtcaatacct
    19861 taacagcttt aggtatagat gcatctagaa taaaagctta tgtgccaagc tctagctcag
    19921 caacgggctt tgtgccaatt cattctccta gttctaaagc tattgtcagt ggtcaatctc
    19981 cttactatgg aacgtctttg agtctgtctg ataaaggaga gcttagtctt tacctttatc
    20041 cagaagaaac acactcttct agtagctcat cgagttcaac gtcaagttca aacagttctt
    20101 caacaaatga tagtactgca ccaggtagca acactgaatt aagcccatca gaaactactg
    20161 ctcaaacacc ttaacaaaga atagttagtc tcatgtttta ctggcatgcc actaactatt
    20221 ctttttaact actcgtcata taaagatgca attttccagg aattctgata aaatacaaag
    20281 aaataagggg aaactaatga aaaaatttca attcttttta cttatcgagt gtatactgct
    20341 agctatgggt atcatgacaa tcttggataa tgatttatca agttttatcc taattctcgt
    20401 tcttatctta ttagcattac gcttttataa ccaagatagc cgcaataatt ttttgctaac
    20461 tgtcagttta ttgtttcttt ttttaatttt catgcttaat ccctacatta tcatggctgt
    20521 tcttttgggg atagtttaca ttttcattaa ccatttttca caagtcaaaa agaagaatcg
    20581 ctttgccttg attcgtttca aggaagaaaa gattgaagtg aataatacca agcatcaatg
    20641 gattggtact gctaattatg aaagtgatta ttattgtttt gatgacatta acatcattcg
    20701 aatctcagga aatgacacgg ttgacttaac caatgttatt gtgacaggaa tggataatat
    20761 cattgtgata cgtaaaattt ttggaaatac gactatattg gtacctattg atgtcactgt
    20821 tactttagat gttagttcca tatacggaag cgttgatttt tttagatgtc agcaatatga
    20881 tttgcgcaac gaatctatta agttaaaaga aacggataac caatccctta aaaaagtcaa
    20941 aattatcgtt acaactattg ccggaaatgt tgaggtggat aggcgatgaa aaaacgttac
    21001 tatgctcttg tttggctcta ctcaaccatt accattttat ctattgtttt tgtggtcatg
    21061 gataatttag gaatcacttt taactacctt cgcaaccatt tatggcaggt tgaacgtcta
    21121 gggttttcga ttttattgtt gattgtttct gtgactttat tattgttgtt attgtggatt
    21181 attatggatg ataacagtaa gcgtaacatc aaccaaaatc taaaatatat tctcaataat
    21241 cgacgactct atctagatga gacatcagaa atcaatacta atttaagtcg actttctaaa
    21301 aaaatgtctc acctgactgc taacatgcaa aagaaagaaa gtgcttatat tcttgatagc
    21361 caagaagttg taaaacaaga gcgcaaacga attgcaagag atttacatga cacagtcagt
    21421 caagagctat tcgcttcgtc attgatttta tcaggaattt cgatgagttt ggaacaactg
    21481 gacaaaacac aattacaaac acagttaaca acggttgaag caatgttgca aaatgctcaa
    21541 aatgatctac gcattctcct tttgcatctt agacctaccg agctagctaa tcggacttta
    21601 tctgagggcc ttcatatgat tcttaaggaa ttaacagata aaagtgatat tgaagtcatt
    21661 tataaggaaa ccattgctca gcttcccaaa acaatggaag ataatctttt tagaattgcc
    21721 caagaattca ttagcaacac gttaaaacat gctaaagcta gtcgaattga agtttatctc
    21781 aatcaaacct caacagaatt acaattgaag atgattgatg atggtgtagg atttgatatg
    21841 gatcaggtaa gggatttgag ttatggtctg aagaatattg aagaccgtgt caatgattta
    21901 gcaggaaacc tacatttaat tagtcaaaaa ggcaaaggag tttccatgga tattagactg
    21961 ccgatagtga agggagatga cgatgagtaa gataaaagtg atattggtcg atgatcatga
    22021 aatggtccgc atgggactca agagtttttt gaatttacaa gctgatattg atgtcgttgg
    22081 tgaggcctct aatggacgtg aaggggttga tttggcattg gctttgaagc cagatgtttt
    22141 ggttatggat ctagtgatgc cagagttagg cggtgttgag gcaactttag aagtcctaaa
    22201 aaaatggaaa gaggctaagg tacttgtgtt aacttcctat ctagataatg aaaagatata
    22261 ccctgtcatt gatgcaggag cgaagggtta tatgttaaaa acatcgagtg cggctgaaat
    22321 tttaaatgcc attcgcaagg tttcaaaggg agagttagct attgaaacag aagttgacaa
    22381 aaaaattaag gcgcatgatc aacaccctga cttgcatgag gaactaacag cgcgtgagta
    22441 tgatatttta caccttttag ctaaagggta tgataatcag accatcgctg atgaactctt
    22501 tatttcctta aaaaccgtca aaacacatgt gtccaatatt ctagccaagt tagaagttga
    22561 tgaccgaacc caagcggttg tctatgcttt tcgacaccat ttagtccccc aagacgataa
    22621 ttaatgctat aatatagggt atagcaaatt ggaggaaagt atggacgcaa aactaaaata
    22681 caaagctaaa aaaatcaaaa tggtgttctt tgatattgat gataccttga gggtcaaaga
    22741 tacgggttat atgcctgagt cgattcaaag agtatttaag gctttgaagg caaaaggcat
    22801 cttggttggt attgcttcag gtcgcgctcg ttacggtgtt ccacaagaag ttcaagattt
    22861 acatgctgac tattgtgtca aattaaatgg agcttatgtc aaagacgatg ctaaaactat
    22921 tattttccaa gctccgattc cagcagatgt tgttgtggcc tataaaaaat gggcggatga
    22981 tatgggcatt ttttatggaa tggcgggtcg tcatgaagcg gtgttgtccg ctcgaaatga
    23041 catgattagc aatgctattg ataatgtcta cgctcaacta gaggtctgtc cggattataa
    23101 tgagtatcat gatgtttacc agatgtggac tttcgaggac aagggagatg gcttgcagtt
    23161 gcctgctgaa ttagcagagc accttcgttt ggtcagatgg cacgataact catcagatgt
    23221 tgtcttgaaa ggtacgtcaa aagcgctagg tgtttcaaaa gtagttgacc atttaggatt
    23281 aaaacctgaa aacattttgg tgtttggtga tgaactaaac gacttagagt tatttgacta
    23341 tgctggtatc agtattgcaa tgggagtatc acacccacgt ttgcaagaaa aagcagatgt
    23401 tatcacaaaa aaagttgaag aagatggcat tttatatgcc ttggaggaat taggattgat
    23461 tgacaaagaa ttacagtttc cacaattgga tttggcaaac cataaaggac caaaagcaac
    23521 cattaaaaca aaccatggcg atatgaccct ggtcttgttc ccagagcatg cccctaagac
    23581 ggttgctaac ttcttaggat tagctaaaga agggtactat gatgggatta tcttccaccg
    23641 tattattcct gaatttatga ttcaaggggg cgatccaaca ggaacaggaa tgggtggaca
    23701 atccatctat ggtgaaagtt ttgaagatga attttcggat gaactttata atctccgtgg
    23761 ggccttgtcg atggctaatg cgggaccaaa tactaacggc agtcagtact ttattgttca
    23821 aaatagtaag gttccttatg ccaaaaaaga actagaacgt ggtggttggc cggctccaat
    23881 tgcagcttct tacgctgaaa atggtggaac tcctcacctt gatcgtcgtc atacggtctt
    23941 tggtcagctt gtgaacgaaa cttcattcca agttttggat ttgattgctg gcgttgaaac
    24001 tggcgcacag gataaaccta aagaagacgt tatcattgaa acaatcgagg tatttgacta
    24061 atgaaaattg gcgacaaact gcatgggacc attacaggaa ttaaaccata cggtgccttt
    24121 gttgcccttg acaatgggac aacaggtctc attcatattt cagaaataaa aacgggattt
    24181 attgatgata ttgatcaatt attggctata ggcaaccagg ttttagtaca agtaattgat
    24241 attgatgaat atagtaaaaa ggcaagttta tctatgcgga ccttagcaga agaaaaacag
    24301 cattttttcc atcgtcatcg ttattccaac agtcggcaca agattggctt taggccactt
    24361 gaagagcagt tacctcagtg gattgaagaa agcctacagt ttttaaaaaa gaacactaaa
    24421 aagagtctgg aaccttagta gttccaaacc cttttttatt tcgtctcaat tactggtaca
    24481 tcatagagtt cagtcgataa atagcgttcg ccattatctg gtaaaatagt taatacatgt
    24541 ttgccttttc ctaactgttt agcgacttca atagcggcgt aaagagcagc tccagaagaa
    24601 atcccaacca ggaagccttc cttagctcct gttcgtcgag cagtttctaa agcatctttc
    24661 gatttaacac ggataatttg gtcataggct ttggtatcta acgtgtttgg gataaatcca
    24721 gctgatatac cttggatttt atgtggtcca ggctcttgac cagataagac agcagattct
    24781 tcagcttcaa cagcatagat agcagtttca gggctagctt ttttcaagac atgtgaaaca
    24841 ccagaaagtg ttcctccagt accaacacca gatacgaatg catctaaaga aatctcctta
    24901 aaagcttcca aaatttcttg agctgttgtt ttttcatgga tgcttggatt ggcagggtta
    24961 ttaaattgca taggcatcca agcacctagt tctattgcta aagtttcagc ttttgcaata
    25021 gcccctttca taccttctgc tccaggtgtt aagacaagct ctgcaccata agcctgaatg
    25081 atttgtcgtc tttccaagct catcgtttcg ggcataacaa taatgactcg atacccttta
    25141 gcagcaccta cccatgcaag accaatacct gtattaccgc ttgttggttc gataataacg
    25201 tcaccaggac ttatcagacc ttcagcttca gcagcttcaa tcatcgataa agcaatacga
    25261 tctttaacag aagatcctgg gttaaaagct tctaatttta cataaacgtc agcagcttcg
    25321 tttggaatta aacggttaag tttgataata ggtgtttgac ctactaattc tgttatagtt
    25381 ttgtaaattt tagtcatagt ttagcctctt ttcttgatac aaaataaata cgctgtgtat
    25441 ttaagtataa ggagagatat tattaaagta aaatacagat ttactatcac tctgataata
    25501 aagccatatc acttttgttg atgattaatc acaggggaat tcaataactt tggatccgat
    25561 tttctcacag cttatctgac catgataata atttgttaaa gtatcaatga gattttctac
    25621 acgttcagta tcacaataaa gtgttgtctt tatcgtgtct gaaaacttag tttctgtttc
    25681 ggttaaagcc aattgatcta gcaagttaga atatagttga tattggggat aagaaagtgt
    25741 gatttctagt cctatctgct gtttaacttc aatgatgcca aatcgtttaa tagctgttgc
    25801 ggtcatgcta gagtatgctc tgattaaccc acctgttcct aatttaatac caccaaagta
    25861 acgcgttacc acaactacta catttgttaa acactgtttt tctaacacag aaaggatagg
    25921 tattccagca gttcctgaag gttcaccatc atcacttgag cgcttgatct gactgttgtt
    25981 accgataatc atcgcaaagc aggaatgatt agctttgtaa tgttcttttt tgatagcatt
    26041 aacaaaattc ttaccatctt cttcagtgct cactcgtttg atatgacaaa tgaaacgaga
    26101 ttttttaata ctttcttcga aaaaaccact ggctttgatt gtcttaaaat gttccataaa
    26161 aaaattgtac aagaaaagat aaaaattagc aaaaaaagcg aataataaag tatggagtct
    26221 attgaaaata gttatgggcg gttgttgcta gaaagccagt taccagattc agcaaggcaa
    26281 cttgctcaac ctttaaaaag tgtagtaata tcgagaggaa aaatgatttg tcaacgttgt
    26341 cactatcagc ttgatgaaga agcacgattg ccaagtggag cctattattg ccgattttgt
    26401 cttgtctttg gacgaaatca atcagataaa ttgatctatg ctatcccacc tatgcatttt
    26461 ccaaaaggta attatctggt ttggagcggt caactaactg cttatcaaga gatgatttcc
    26521 caacagttat taatcaatat gcaaaatcaa aaaacaacat tagttcatgc tgtaactggt
    26581 gcaggaaaaa cagaaatgat ttatgctgcg attgaggcgg taattaacac aggtggctgg
    26641 gtttgcattg ccagcccaag agtagatgtt tgtgtagagg tggctactcg tttatcccaa
    26701 gccttctcct gcagcatctg tttaatgcac gcagagtctc ttccttatca aagagctcct
    26761 attatagtag ccacaacgca tcagctgcta aaatttcaca aggcttttga tctcttaatt
    26821 attgatgaag tagacgcatt cccttttgta aataatattc aacttcatta tgcagcaagt
    26881 caggctttaa aagaaggtgg cgcaaagatt ttattaactg caacttcaac tagaacttta
    26941 gaaagaaagg taaacaaggg agaagtagtc aagctgacat tggcgagacg ctttcataat
    27001 aggccattag ttattccgaa atttataaga agttttaatc tatttaaaat gatacatcgg
    27061 caaaaacttc ctcctaaaat tttaaaatac ttaaaaaaac aaagaaaaac aggctatccc
    27121 cttttaattt ttctacccac tattataatg gcagaatctg ttacagcaat cttaaaagag
    27181 ttattgcccg ccgaacaaat agcttgtgtt tctagtcaat cacaaaacag gaaagaaggt
    27241 atcacagctt ttcgtcaagg gaaaaaaacg attttaatta caacaacaat tttagaaagg
    27301 ggtgtgacat ttccccaaat tgatgttttt gttcttgggt ctcatcatcg tgtctatagt
    27361 tcccaaagtc ttgttcaaat tgctggacga gttggaagat ctatagacag accagacgga
    27421 acgttatatt tctttcatga aggtatcagt aaagcaatgc tactagcaaa aaaagagatt
    27481 aaagaaatga attataaagg gtattcgcat gatctgtcta ctatgtcaac aaattagtca
    27541 aacaccaata agtattacag aaatcatctt tttaaggcgt atctcttcac cgatttgtca
    27601 acaatgtcaa aaaagctttc aaaagatagg aaaaagtgtt tgtgcgacat gttgtgcaaa
    27661 ctcagatata atagcttgtc aagattgtct aaaatgggaa aacaaaggat acaatgtaaa
    27721 tcatagaagc ttatattgtt ataatgctgc tatgaaagca tacttcagtc aatataagtt
    27781 tcaaggagac tatttattaa gaaaagtttt tgcagtagaa cttgccgatg ttatcaccaa
    27841 gtactataaa ggctatatcc cagtcccggt tcctgtaagt ctcggttgtt ttcgagaaag
    27901 acaatttaat caagtgagcg ctattcttga ggcagctaat gttagctacc tttctctttt
    27961 tgaaaagcta gataatactc accaatcttc cagaacaaaa aaagagagat tattagtaga
    28021 aaaatcttat cgactactaa aagtatcaaa cattcctgat aaaatcctta tagtagatga
    28081 tatttatact actggtagta caattatcgc tcttagaaaa caattggcta aagtagcaaa
    28141 tagtgacatt aaaagtttgt caattgcacg ttaaaacttg caaattttca tgaaagcagt
    28201 ataatagtat taaagataaa gtaagcgctt caagcgcgag aaagaggtat cctatgatta
    28261 agttcagtat tcgtggagaa aatatcgaag tgacagaagc tattcgcgat tatgtagaat
    28321 caaagcttac taaaatcgaa aaatattttg ctaaagacca agaaatagat gcacgtgtta
    28381 atttaaaagt ttatcgggag agatcttcaa aagttgaggt aacgatccca ttagattcag
    28441 taacccttcg tgctgaagat gtttcacagg atatgtatgg ctctattgat ttagttgttg
    28501 ataagatcga acgccaaatt agaaaaaata aaaccaaaat tgctaaaaaa catcgcgaaa
    28561 aggttccaac tggtcaagtc tttacaactg agtttgaagc tgaagaagtc gatgagatac
    28621 cagaagtaca agttgtacgt actaaaaatg tgacattaaa accaatggat gttgaagaag
    28681 cgcgtttaca gatggaacta ttgggacatg atttctttat ctacactgat tctgaagatg
    28741 atgcaacaaa cattttatac cgtcgcgaag atggaaacct aggattaata gaagctaaat
    28801 agttcacaac taaaacctac agggatgatt atacgtattt taaaagtggg aaaaagctat
    28861 tcatgtttag aggtgacatg atgaaaatga gattaagtga tagatacgac agtcattatg
    28921 ctaacaaaag tatctattac agttcctctt taagtaaatc ttacgtaaaa ttagtttgaa
    28981 aaaagattag tcccaattct gggctagaaa ttctcaaaaa atatgaaaaa gcgcgttttc
    29041 agaatataaa gcgcgttttt tctttttcca t
//
LOCUS       DAUGFP010000017        29051 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.017, whole genome shotgun sequence.
ACCESSION   DAUGFP010000017 DAUGFP010000000
VERSION     DAUGFP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29051
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.017"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1201
                     /gene="sstT"
                     /locus_tag="VH663_000814"
     CDS             <1..1201
                     /gene="sstT"
                     /locus_tag="VH663_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEQ8174030.1"
                     /translation="NLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLFVGGLK
                     AIAPLLVFALVSQAISHQKKGRQTNMTLIIFLYLFGTFASALVAVLTAYLFPLTLVLN
                     TPVNTELSPPQGVVEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKAASQET
                     KHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLVGTMVF
                     VALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKIGLSKD
                     TYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSACGASGV
                     AGGSLLLIPVACSLFGISNDLAMQVVGVGFIVGVIQDSCETALNSSTDVLFTAIAENA
                     FWKRKKA"
     gene            complement(1256..1930)
                     /locus_tag="VH663_000815"
     CDS             complement(1256..1930)
                     /locus_tag="VH663_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEQ8174031.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(1940..3331)
                     /locus_tag="VH663_000816"
     CDS             complement(1940..3331)
                     /locus_tag="VH663_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEQ8174032.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSIEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(3401..4114)
                     /gene="rsmG"
                     /locus_tag="VH663_000817"
     CDS             complement(3401..4114)
                     /gene="rsmG"
                     /locus_tag="VH663_000817"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEQ8174033.1"
                     /translation="MTPQDFYRTLEKDGFLLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEKNEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            4264..4821
                     /locus_tag="VH663_000818"
     CDS             4264..4821
                     /locus_tag="VH663_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEQ8174034.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            4868..5764
                     /gene="htpX"
                     /locus_tag="VH663_000819"
     CDS             4868..5764
                     /gene="htpX"
                     /locus_tag="VH663_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEQ8174035.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMIKALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
     gene            5998..6531
                     /locus_tag="VH663_000820"
     CDS             5998..6531
                     /locus_tag="VH663_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEQ8174036.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPICLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            6798..7484
                     /gene="covR"
                     /locus_tag="VH663_000821"
                     /gene_synonym="csrR"
     CDS             6798..7484
                     /gene="covR"
                     /locus_tag="VH663_000821"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEQ8174037.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            7490..8992
                     /gene="covS"
                     /locus_tag="VH663_000822"
                     /gene_synonym="csrS"
     CDS             7490..8992
                     /gene="covS"
                     /locus_tag="VH663_000822"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEQ8174038.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHEVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            9207..9701
                     /gene="nrdR"
                     /locus_tag="VH663_000823"
     CDS             9207..9701
                     /gene="nrdR"
                     /locus_tag="VH663_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEQ8174039.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVIQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            9685..10860
                     /locus_tag="VH663_000824"
     CDS             9685..10860
                     /locus_tag="VH663_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEQ8174040.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAASIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGNLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            10861..11763
                     /gene="dnaI"
                     /locus_tag="VH663_000825"
     CDS             10861..11763
                     /gene="dnaI"
                     /locus_tag="VH663_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEQ8174041.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLSDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            11826..13136
                     /gene="der"
                     /locus_tag="VH663_000826"
     CDS             11826..13136
                     /gene="der"
                     /locus_tag="VH663_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEQ8174042.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVISAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            13343..16441
                     /locus_tag="VH663_000827"
     CDS             13343..16441
                     /locus_tag="VH663_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8174043.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASDTDKGDNENIFPNHARHL
                     RLPSGFFEEGVHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMQDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGESGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNESRASMSIEEIKEILGLN
                     TH"
     gene            16685..17287
                     /locus_tag="VH663_000828"
     CDS             16685..17287
                     /locus_tag="VH663_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174044.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            17327..18655
                     /locus_tag="VH663_000829"
     CDS             17327..18655
                     /locus_tag="VH663_000829"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEQ8174045.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEKGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDMALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            18701..19183
                     /locus_tag="VH663_000830"
     CDS             18701..19183
                     /locus_tag="VH663_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8174046.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            19295..20869
                     /gene="mltG"
                     /locus_tag="VH663_000831"
     CDS             19295..20869
                     /gene="mltG"
                     /locus_tag="VH663_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEQ8174047.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAARRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKDAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAFTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            20942..21424
                     /gene="greA"
                     /locus_tag="VH663_000832"
     CDS             20942..21424
                     /gene="greA"
                     /locus_tag="VH663_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEQ8174048.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(22048..22971)
                     /gene="yidC"
                     /locus_tag="VH663_000833"
     CDS             complement(22048..22971)
                     /gene="yidC"
                     /locus_tag="VH663_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ8174049.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMTFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMSIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKNPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(23053..23331)
                     /locus_tag="VH663_000834"
     CDS             complement(23053..23331)
                     /locus_tag="VH663_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEQ8174050.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGGIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGN"
     gene            23592..24329
                     /locus_tag="VH663_000835"
     CDS             23592..24329
                     /locus_tag="VH663_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEQ8174051.1"
                     /translation="MVITSKANQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            24368..24868
                     /locus_tag="VH663_000836"
     CDS             24368..24868
                     /locus_tag="VH663_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ8174052.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            24883..25572
                     /locus_tag="VH663_000837"
     CDS             24883..25572
                     /locus_tag="VH663_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEQ8174053.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            25750..25992
                     /locus_tag="VH663_000838"
     CDS             25750..25992
                     /locus_tag="VH663_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEQ8174054.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            26170..26964
                     /gene="racE"
                     /locus_tag="VH663_000839"
     CDS             26170..26964
                     /gene="racE"
                     /locus_tag="VH663_000839"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEQ8174055.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSVQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLAPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVEHRFFTTANSEIFQEIASIWLKQKINVEHVTL"
     gene            26961..27947
                     /locus_tag="VH663_000840"
     CDS             26961..27947
                     /locus_tag="VH663_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEQ8174056.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQREIKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDTILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            27926..28447
                     /locus_tag="VH663_000841"
     CDS             27926..28447
                     /locus_tag="VH663_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEQ8174057.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYSSLSKEFKR"
     gene            28444..28905
                     /locus_tag="VH663_000842"
     CDS             28444..28905
                     /locus_tag="VH663_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ8174058.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            28902..>29051
                     /locus_tag="VH663_000843"
     CDS             28902..>29051
                     /locus_tag="VH663_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEQ8174059.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLVGERVNQDKLLL
                     YQNSIA"
BASE COUNT         9332 a         5003 c         5900 g         8816 t
ORIGIN      
        1 taatttatgg gttagggtta gtctaatcaa aaaaataggt atcggtgttg taataggagt
       61 catgttgggc attttggctc ctgatcttac aggttttagt attttaggaa agttatttgt
      121 tggtggttta aaggctatcg cacctttgct tgtatttgct ttggtttccc aagccatctc
      181 ccatcagaaa aaagggagac agactaatat gacattaatc atttttctgt atttatttgg
      241 tacctttgcg tcagctttag tagcagtact gacagcttat ctattccctt tgacgttagt
      301 gttaaataca cctgtaaata cagaattatc accacctcaa ggtgttgttg aagtttttca
      361 gtcacttcta ttaaaattag tggataatcc gatcaatgct ttagctactg ccaattatat
      421 tggtgtgcta tcatgggcaa ttatttttgg actagcatta aaggctgcaa gtcaagaaac
      481 aaagcatctt ataaaaacag cggcagaagt tacttcacaa atagttgttt ggatcatcaa
      541 tctggcacct attgggatta tgagtctggt gttcactacg atttctgaaa acggtgttgg
      601 aatcttatct gattatgctt ttttgatact agttttagta ggaacaatgg tttttgtcgc
      661 cttagtggtt aatccactta ttgcggtttt gattacacga caaaatcctt atccccttgt
      721 cttgagatgt ttacgtgagt caggtcttac agcatttttc acacgaagtt ctgcagccaa
      781 tattccagtc aatatgcaat tatgccaaaa aattggattg agtaaagata cttattcagt
      841 gtcaattcca cttggagcaa ctattaatat gggtggggca gcaattacca tcaatgtcct
      901 aacgcttgct gctgtacaca cttttggtat tcctattgat tttctaacag ccctcttact
      961 tagcgttgtt gctgctgtct ctgcttgtgg tgcttctggt gttgctgggg gatcgttgtt
     1021 acttattcct gtagcatgta gtttgtttgg catttcaaat gatcttgcga tgcaggtggt
     1081 tggtgttggt tttattgttg gtgttatcca ggattcttgt gagacagcct taaattcgtc
     1141 aaccgatgtt ctttttacag ctattgctga aaatgctttt tggaaacgta aaaaagcata
     1201 gctagattta acatagtaaa aaacatcctt ggaaaacgcc aaggatgttt ttttattatt
     1261 taagatagcc aagataatca aatttttcaa aggtgtggtc attagcgatg gccacaatga
     1321 tagtattcgg ttctaaaggt tcaaaaggtt tgacattagt atctaaggtt ttaacttctt
     1381 tttgacgcat gccaatcaca tttaactcat atttacgacg aacatctaac tctgaaagag
     1441 attgtccttc ccagctttta ggaatgacaa attcaatcat cgaaatacca tgttccaaat
     1501 agatgatgct ttcaatatgt cgtctgagaa ggtttgaagc tacacgtttt ccagaatcac
     1561 gttcaggggt gatgacttta gttgctccga tgccatatag cacttcttca aaaattttat
     1621 ttttagcctt ggcaataatg gttggaacac cgagtttttt acagtgcatg actgcaagaa
     1681 cagaagattc caagttattc cctgaagcga taacgacagt atcacaatgt tcaatcccta
     1741 cagctaatag aaattctttg tcagtaatat ctccaacagc ggctttagtg accaagtcgg
     1801 ccacctcttt aacatgactt tctctgatat caatagcaat aacgtcttga tcaaaattac
     1861 ttaattctct ggcaacagta cggccaaaaa taccaaggcc aagaacgccg acagttttac
     1921 gttttaacat gttttctcct taacccacta gaatatcagt agtggcatat tgaatggttt
     1981 tttcttttcg ttgaatcaga ctgatgagga cagtaattgg gccaacgcgg ccgacgaaca
     2041 tcaagacaat gacaataaga cgtccagcag tagacaattg tggtgtcaaa tccatagata
     2101 ccccaactgt cgcgatggca gaaatagatt caaataagag tggtatagga gcgatatgag
     2161 gctccacgct aagtaataaa ataaaaccta tcataagaac tgcaaaaaag aagataagaa
     2221 ctgtcatggt ctgcttaatt gtcttatttg cgataattcg gttacgaaag gtaacttctg
     2281 attgccctga aagttccgct ttaaagagta agaaagtaat ggcagcagtc gtcactttaa
     2341 taccaccagc tgttccacca ggagcaccac caataaccat ttgaatcata taaagaatgt
     2401 tagtcggtgc tagggtatcg ttataagaga ttgttgcaaa gccagctgtt ctcatagtga
     2461 ctgtttgaaa aaatgaaacc ataagttgct gatgtaaact aaaattggca atggttttgc
     2521 tgttatcttt ttctaaaaac caggttaaga aggttccaag aaaaagaata acagctgtgg
     2581 tttgtagtac tagacgagac tgattggata atttacgaaa agttgcccca taacaatgag
     2641 gtctttcaaa aaagtatttt ttaaaagcta ctcctaaatc aacccaaacc gcaaaaccaa
     2701 gtccaccaga gataatcaag aacgtaatga taacatttaa tgtaggattt aacataaagt
     2761 cttttaaact agaagatcct aaattgtcaa aaccagcgtt acaaaatgcc gacacagcta
     2821 aaaaaatact attgaagata ccatttttcc acccgaagcg ggggataaag tcaatcatga
     2881 taactatcgc agcaaaggct tctatactaa aggttacttt ataagcaaaa aaaaggtagt
     2941 gctttaaatc tttgctatca cctcgattta acgctgattg gagcaaggtt tgatcgctca
     3001 atcgcatttt tcgcttgagg gcaaaagtac tcacagctat taacgtcacc aagccaagac
     3061 caccaatctg cattagtgcc atagcaatgg tttgtccgat accgttatac acttcagcga
     3121 cgggaacaac agaaagtcca gtcacacaaa ccatggaaac cacattaaag aaatgatcaa
     3181 gatagacggt attaggacca ttttgataat gggtaaaggg catggataac aagagagtcc
     3241 caatcagaat cacaatggca aaactaaatg ttaagcgctg tgtgactgaa agagatttga
     3301 taaaagagcg tttcatgttt cctccagaca taatatctcc tactataata acaaaaataa
     3361 gttaaaaaag ctaagtttca tcttagcctt tggtactatt ttatagaggt tttttgttag
     3421 gcaagccagc ttttcttgga tacttgttag gtgtttcctt tttcttctca acaatggtga
     3481 tgaagcgaga atctccattt ggcaattggt agctatggtt tttaatgact ttaccaaaaa
     3541 gaagacaaag agcattctta gcttcttcta actcttggtc agcagcttgt gcctttaagg
     3601 cgattaattt tcctccaatt tttaaaaagg ggatggttaa ttcagataga acctgcatcc
     3661 gagcaaccgc tctggcagta acgacatcaa attgacctcg aaaggccttg tcctgtccga
     3721 aatcttctgc tctaccatga aaaaaatgga cattttccaa gcccaattcc tgagcaagga
     3781 gtgtcagaaa agaaatccgc ttattaagag aatcaataat ggtaacctca aggtttggga
     3841 aaagaatttt catgggtagg ctaggaaaac cagctccagc tccaatgtcc aataatttga
     3901 ttggttcatt agctaaaaag ccctgtaaaa taggagcgat ggaatcataa aaatgcttga
     3961 ggtaaacttc atttttttct gtaatagctg ttaaattaat tttagtgttc cattccacta
     4021 gtaatttgaa gtaggtgtca aattgctctt tttgcttcga tgataataaa aaaccatctt
     4081 tctctagtgt tcggtaaaag tcttggggtg tcattagtgt cctctgtttt tgatagttgt
     4141 taccatttta tcataaaata gcctataaca aaatccaata aatcatcaga aataacacct
     4201 cattacttgc tattagccta gaaattgcta taattgagac acaaagatta aaggagaccc
     4261 actatgccaa cagttttgat tatcttagtc gttttaggag tgcttgcgct atggttaatg
     4321 attagttaca atagcctggt taaatctcgg atgcacacaa aagaagcttg gagtcagatt
     4381 gatgtgcaat taaagcgtcg taatgacttg attccaaatc ttattgaaac cgtaaaggga
     4441 tatgctagct acgagcaaaa gacatttgaa aaaatcactg atttgcgtgc gcgtgttgcg
     4501 aatgcctcaa ctcctcaaga aaccatggcg gcttctaacg aattgagtaa acaagtgacc
     4561 agtttgtttg ccgttgctga aaattaccca gacttaaaag ctaacgaaaa cttcttgaaa
     4621 ttacaagaag agttgaccaa tacggaaaat aaaatctcat attctcgtca actctataat
     4681 tcaacaacgt ctaattacaa ccttcaatta gaatctttcc caagcaatat cgctggtaaa
     4741 ttatttggtt ttaaaccaag tgaattctta caaacaccag aagctgaaaa agaagttcca
     4801 aaagttgaat ttaactttta aatatgaaat tatcatctag taggcaatca taagagaaag
     4861 gagaattatg ctttatcaac agatttcaca aaataagcaa cgaacagttg ttttattagt
     4921 tgttttcttc gctctcttag cacttattgg agcgtcagca ggctatctgc tgttagataa
     4981 ttatgccatg ggcttggttc ttgctcttgt cattggggtg atttatgcta ccagtatgat
     5041 ttttcaatcc accagtcttg taatgagtat gaacaatgct agagaagtta cagaaaaaga
     5101 ggctccaggc ttcttccata ttgtcgagga tatggctatg gtggcccaga ttccgatgcc
     5161 gagagttttt attattgaag atccttcttt aaatgctttt gcgacaggat ctagccctca
     5221 aaatgctgct gttgcagcaa cgacaggttt acttgaagtc atgaatcgtg aagaacttga
     5281 aggtgttatc ggccatgaaa ttagccacat ccgaaattat gatatccgta tttcgactat
     5341 tgctgtagct ttggcgagtg ctgttaccgt tatttctagt atcggtgggc gtatgctatg
     5401 gtatggtggc ggttctcgca ggcaaagaga tgacggagat gatgatgttt taagaatcat
     5461 tacgttactt ttgtcgttat tatctttgct tttagcgccc ctagtagcaa gtttgattca
     5521 gttggctatt tctcgtcaaa gagaatatct agctgatgct agttctgttg aactaacaag
     5581 aaatccccaa ggtatgatta aggctcttga aaaattgcag ttatctcagc caatgaagca
     5641 tcctgttgat gatgctagtg cagccttgta tattaatgag cctcgcaaaa aaaggagctt
     5701 cagttcatta ttcagcaccc atcctcctat tgaggagagg attgaaaggt taaaaaacat
     5761 gtgagcgttt ggggtttttg ctcaagcctc acttttttat gtctttgggc agataatcta
     5821 aaatggcagg aattatcaaa aatatctgaa caaaaagaaa aaaatgagtg aaacaatggt
     5881 taaaaataga atgatttaaa tcttaaaatt aaaaaacgtc agaaaataaa tgcaaaacta
     5941 ttgacaaatc ttttagatag tagtaaaaat aaatgagagt tttattagga gaagatgatg
     6001 ttagctattt ccgaaatcag aaaacaccca gacggtctct cgtttgatcg tttatgtgat
     6061 gtcaaaagta tgttacttga gcgtgaccaa cagattattg acataaaggc agttaaagct
     6121 gtcggtaatg ttcgctatga taaggggctt tatctgttag attatcaatt gagttacgaa
     6181 gtgatattac cgtctagccg ttctatggtt ccgatctgtt taagtgaagt ccaacacatt
     6241 caagaattat ttatagaggc gacagattta gctgataaga aagagttggt tgaagacaat
     6301 ctggtattag ttttagacaa agacgctatt aacctcgaag aaagtattgt ggataatatc
     6361 ctacttgcta ttccgctaca ggtcttgaca gaggaagaaa agaaatcaaa agagttgcca
     6421 gcaggtcaaa attgggcagt tttgacagaa gaagactacc agtgtctaaa agaagaaaag
     6481 caaaaagaaa ataatccttt tgctagcttg caagggttgt ttgatgaata aaactgaata
     6541 ttaaagatgt ctgtaatttt tatttgaaaa aacagctatt ctttgatata ctctttagag
     6601 aatatggtta cttatctaaa gtagaaggta ttttgaccat agagggcaaa gaaggtaatg
     6661 actattagat gcttcatatt ttattcgcta gaagactatt tgaccattag aactgaaata
     6721 gtctaggata tgagatgaat ttcatagttt tctttctggg agaaaaagat agattaagag
     6781 gataagggtt ggtataaatg acaaagaaaa ttttaattat tgaagatgaa aagaatctgg
     6841 ctagattcgt ttctcttgag ctgcaacatg agggttacga agtcattgtt gaggtcaatg
     6901 gtcgtgaagg gttagaaact gctttggaaa aagagtttga tttaatcctg cttgacttaa
     6961 tgttaccaga gatggatggt tttgaagtga cccgtcgttt gcaaaccgaa aaaacaacgt
     7021 atatcatgat gatgactgcg cgtgattcta ttatggatgt ggttgcaggt ttagaccgcg
     7081 gtgcagacga ctatattgtt aaaccgtttg ccattgaaga actacttgcc cgtattcgtg
     7141 ctattttccg ccgtcaagat attgaatctg agaagaaagt gcctagtcaa ggcatttatc
     7201 gagatctagt tttaaatcca caaaaccgtt cagttaatcg tggcgacgat gagatttctc
     7261 tcactaaacg tgaatatgat ttgcttaata ttttgatgac taatatgaat cgtgtcatga
     7321 cacgtgaaga attattgtca aatgtttgga aatatgatga agccgttgag actaatgttg
     7381 tagatgtcta tattcgttat ctccgcggca aaattgacat tccaggcaag gaatcttata
     7441 tccaaacagt gcgtggcatg ggatacgtta ttcgtgagaa ataagtcata tggaaaatca
     7501 gaaacaaaaa cagaagaaat ataaaaactc gttaccaaaa cgactatcta atatcttttt
     7561 tgttcttttt ttctgcattt tctctgcctt tacactgatt gcctatagtt caacaaacta
     7621 tttcttattg aagaaagaaa agcagtcagt ctttcaagct gtaaatattg ttagagttcg
     7681 tctttctgag gtggactcta attttacatt agagaactta gcagaagttt tgtacaaaaa
     7741 cgataaaaca catctgagaa ttgatgacag aaagggcagt cgagtcatta ggagtgagcg
     7801 cgatatcaca aatactctag atgcgaatca agatatttat gtctataaca ttgataaaca
     7861 gatgattttt accacagata acgaagaatc atctcctggc ttgcatggtc ctattggtcg
     7921 ggtgtatcac gaccatatcg aagatcagta tcgtgggttt tccatgacac aaaaggtata
     7981 ttctaatcgg actggaaaat ttgtgggcta tgttcaagtc tttcatgatt taggcaatta
     8041 ttatgtcatt agagcaagac tgctgttttg gctactagtt gttgagttat ttggcacaag
     8101 cttagcctat ttaatcatct taattactac gcggcgcttt ttgaagcctt tacacaattt
     8161 acatgaagtg atgcgtaata tctctgaaaa tcctaataat ttaaacctgc gctcagatat
     8221 ttcgtcagga gatgaaatcg aagaattgtc tgttatcttt gacaatatgt tagacaaatt
     8281 ggagacacat actaagttgc aatcacgttt tattagtgat gtcagccatg aattacgaac
     8341 accggttgcg attattaaag ggcatattgg tctcttacaa cgttggggta aagatgacag
     8401 tgatatactt gaagaaagct tgacagcaac agcgcatgag gctgaccgta tggcaatcat
     8461 gattaatgat atgttagaca tgattcgcgt acaaggctca tttgagggac atcaaaatga
     8521 tatgacagtt ttggaagatt ctattgaaac tgttgttggt aattttagag ttttaagaga
     8581 agattttatc tttacatggc agtcagaaaa tccaaaaacg atagcccgta tttataaaaa
     8641 tcattttgag caggctttga tgattctgat tgacaatgct gtgaagtatt cccgtaaaga
     8701 aaagaaaatc gcgattaacc tttcagtgac tggcaaacaa gaagctattg ttagagttca
     8761 agataaaggc gaaggaattt ctaaagaaga tattgaacat atctttgaac gcttttatag
     8821 aacggataaa tcacgtaatc gaacaagtac ccaggctgga ttagggattg gcttgtctat
     8881 tctcaagcaa attgtagatg ggtatcattt acagatgaag gttgaaagtg aattaaatga
     8941 aggttcagtg tttatcttac atattccttt ggcccagtct aaagagagtt agagtagctt
     9001 aaggcattgt taatgtgaca ggtatagtat gtaatcatta gataatagta gcagtctttt
     9061 gccacaactg gttagaagct gatgctattt ttttagcaat gatggatgac atgaaagaaa
     9121 tactgtcaat cgcgatgcct tttgttatcc ttgagaatat ggtaaaatag tgtattagat
     9181 taatttgata gaattggggt gtttgcgtgc gttgtccaaa atgtaattac cataaatcga
     9241 gtgttgttga cagtagacaa gctgaagacg gcaacaccat tcgtcgtcga agagaatgtg
     9301 aacagtgtca tactcgcttt actacctttg agcgtgttga agaactccct ttattagtca
     9361 tcaaaaaaga tggcacacga gagcaatttt ctagagataa aatattaaat ggggttatcc
     9421 aaagtgcgca aaaacgacct gtctcgagca cagatattga aaatgtcatt tctcgtattg
     9481 aacaagaagt tcgaacaacc tacgaaaatg aagtgtcaag tactgctatt ggtaatttag
     9541 tgatggatga gttggccgaa cttgatgaaa tcacatatgt acgctttgct agtgtctaca
     9601 agagcttcaa ggacgttgat gaaatcgaag aacttttgca acaaattacg aatcgcgtac
     9661 gcggaaagaa aaaacgttta aataatgatg aaaccaattg atacttttac atatctaaaa
     9721 cgcaacaaag taactcttga ttcggtgact ttaatacagt tgtactttcc aattattgga
     9781 agtgacgcag cctctatcta tcaatacttt attcactttt ttgacgatgg tttacaacgt
     9841 cataaattca gcgatatttt aaatcaccta cagtttggta tgaaacgttt tgaagatgct
     9901 cttgccatat tgacagcaat ggaattagtg agtgtgtatc agctatcaga tacatatctc
     9961 attacgttgc accagccgct tagccgtgac ttgttttttc aacatccagc ttattctcga
    10021 ttattagaac aaaagattgg agaggtagct gtctctgagt tgcaggtaac tgtcccaagt
    10081 caagcaagaa atatttctaa gcgattttcg gatatttttg gggtgcaagg aaatttgacg
    10141 aacgtacctc aaaagccaca aaagaatttt gacctttcaa gtttccagca attaatggtt
    10201 cgtgatgggt tacaatttga agataatcaa aaagatatca tcagccttta cagtattgct
    10261 gaacagtatg atatgacgtg gtttgatact tatcaaatag ctaaggcgac ggctgttaat
    10321 gggaaaattc gacctgaacg cttattggct aaaaaaaatc aaagcatgac aaaaccaagc
    10381 aaagaaaatt tttcacaggc tgagcaaatt attttgcgag aagcaaagca agacagtgct
    10441 ttagtctttt tggaaaaaat taaaaaggct agacgtgcaa cgattactaa ggatgaacgc
    10501 attttattgc aaacattggc caaaatgaat tttcttgatg atgttatcaa tgtcatggtg
    10561 ctttatacct ttaataagac aaaatctgct aatttacaga agagttatgt cttaaaaatg
    10621 gccaatgatt tcgcctacca aaaggtgtca acggctgaag aagctatagt ggttttgcga
    10681 gccttcacgg atcgtcaaag tcgtcggcaa tcaaaagtaa aaacaagtca aagtaatgtg
    10741 cctaagtgga gcaatcctga ttaccaagag acaacaagtc aagaagagca ggcgaaacta
    10801 gatcagttca aacaggctgc tttgaagagg ttagaaaacc taggaaaagg gggtgactag
    10861 atggaaaaga ttggagaaac catggcgaaa ttgggtcaaa acactcgtgt taatagcgac
    10921 cagttgattc aaaccatttt agcggatcct gaagtagcaa gcttcatcag tcaacatcac
    10981 ttatctcaag agcaaattaa ccttagctta tctaagttca atcaattttt ggtggaacgc
    11041 cagaagtatc aactcaaaga tccctcttat attgctaagg gctatcagcc tattctagct
    11101 atgaatgagg gctacgctga tgtgtcttat ttggaaacca aagaattggt agaagctcaa
    11161 aaacaggcag ctatctctga acggattcaa ctggttagtt tgccaaaatc ctatcgccat
    11221 attcatttat cagatattga tgttaataat gcgagccgta tggaagcctt ctctgctatt
    11281 ttagattttg tggaacaata cccatcagca gagcaaaaag gcttatattt gtacggagat
    11341 atgggaattg gcaagtctta cttgttggct gctatggccc atgaattgtc tgagaaaaaa
    11401 ggtgtgtcaa cgacccttct acattttccg agctttgcca ttgatgtaaa aaatgccatt
    11461 tcaaacggct ctgttaaaga agaaatcgat gcggtcaaaa atgtaccagt gctcatttta
    11521 gatgatattg gtgcagagca agccacttct tgggtccgtg atgaggtgtt gcaggtgatt
    11581 ttacaatacc gtatgttaga ggaattgcca accttcttta cctctaatta cagctttgcg
    11641 gatttggagc gaaaatgggc aaccatcaaa ggaagtgacg aaacttggca ggccaaacgt
    11701 gtcatggaac gcgtgcgtta tttggctaga gagtttcatt tagaaggggc taaccgccgt
    11761 taagtttgag atagcaatca agaagaatag ctacacgtat gagtgagtga aagaaaagga
    11821 gaatcatggt tttacctacc gttgccattg tgggccgtcc aaatgtgggc aagtccacct
    11881 tatttaatcg aatcgcagga gagcgcattt caattgtaga agacgttgaa ggggttactc
    11941 gtgaccgtat ttatgctact ggggagtggc ttaaccgcca attttcattg attgatacag
    12001 gtggtattga tgacgttgat gccccgttta tggagcaaat caagcaccag gctcaaattg
    12061 ccatggaaga agctgatgtt atcgtttttg ttgtttctgg caaagaaggg gttacggatg
    12121 cagatgaata tgtgtctaaa atcctatacc gaaccaatac tccagtgatt ttagctgtca
    12181 acaaagtgga taatcctgaa atgcgtaatg acatttatga tttttattca cttggtttag
    12241 gagatcccta tccagtttct tctgttcatg gtatcggaac aggggatgtc ttggatgcga
    12301 tcgttgaaaa cttgccagta gaagaggctg aagaaaatga tgatattatt cgcttcagtt
    12361 taatcggtcg tcctaatgtt gggaaatcta gcttgattaa cgctatttta ggtgaggacc
    12421 gtgttatcgc tagccctgtc gcagggacaa cacgtgacgc tattgacacg cattttacgg
    12481 atgctgacgg tcaagaattt accatgattg acacggctgg aatgcgcaaa tctggcaaga
    12541 tttacgaaaa tacagagaaa tattctgtca tgcgtgccat gcgtgccatt gaccgctccg
    12601 atgtggtctt gatggttatc agtgctgaag aaggtatccg tgaatacgac aagcgtatcg
    12661 ctggctttgc acacgaagct ggtaaaggaa tgattatcgt ggttaacaaa tgggatacca
    12721 ttgacaagga taatcatacc gttgctaagt gggaagccga tattcgtgac caattccaat
    12781 ttttgacata cgctccgatt atttttgtgt cagctttgac gaagcagcgc ctcaacaaat
    12841 tgcctgactt gattaaacgt atcagtgaga gtcagaataa acgtatccca tctgctgtcc
    12901 tcaatgatgt tatcatggat gctattgcca ttaatccaac accaacggat aagggtaaac
    12961 gtttgaaaat tttctatgcc actcaagtgt cagtaaagcc accaacattt gtcgtctttg
    13021 ttaacgagga agaattgatg cacttctctt atctacgctt cttggaaaac cagattcgcg
    13081 cagcctttac ctttgaaggg acaccgattc atctgattgc tcgcaaacgg aaataagaaa
    13141 tgaacatcaa ctaaaatgct gacctaagaa ataatctcat cattgataag gactgtgttg
    13201 tgtatgttac aggagacagt cctttttata atacctttaa gggtgtagac ggaagattag
    13261 tagcaaacaa agaaaaagga ttttgacaag aaatatcagt cggtttttgc tataatgaaa
    13321 ggataagcaa aaaggtggaa aaatgatggc tagattaatt ccaggacgag taaggaatga
    13381 aggtattaag ctttatgagc aaggtttagt ctcattccaa gatgataata aaggcattct
    13441 tcaaatagaa gtggagactt atcaggttca atatggagca gatgatgagg atatcacttg
    13501 tcaatgcgac acatttcata tgaaacatta ttgcaagcat attgcagcag tcgagtattt
    13561 tttaaaaaat gatcaaaaag gtaagctttt tttaaaacag ttaacaaatc aaacaaaaat
    13621 caaagaaaca acaaagaaaa tgacatcttt tggtagcctc tttttagata gcttagccat
    13681 gaatgaagat gattctgtta aatacaggct gtctgctctt ggtagtcgaa gccctttttc
    13741 gtcagattac tggtggagtc tgaaaatcaa tcgtttacca gatgatcgtt cttatgtgat
    13801 tcgagatatt aaaggctttt tgcagcttat taaaaaagaa ggcttttatc agattggaaa
    13861 aaattatttt gaacaacttt catggctgca gtttgaccca tccagtcaag accttattga
    13921 gtttttatgg cgcttagcat ctgatactga caaaggggac aacgagaaca tttttccaaa
    13981 tcatgcaagg catttgcgct tacctagtgg tttttttgaa gaaggcgttc actatttgac
    14041 gtcactttat gattttactt ttgaggggcc ttctcaaact tatcatcatc tttttgtccg
    14101 ttctttggaa gcagaggcag gcctttatga gtttaaagtg gaagtgcatc gcaaatctat
    14161 tgaattgcaa attgctgaaa aaaatgtgca atacctcttt gataatgact atcttcttta
    14221 tcaagatacc ttttatcact tgacgttaaa gcaacgaaaa atggttcagg ctattcgtag
    14281 tctgcccatt gaggccgact tggctaaaca tattcatttt gatttggatg atcatgcgaa
    14341 attagctgca agcttatcag attttaaaca gatcggtcta gtagaggctc ctaaaagctt
    14401 tgctattaga gactttgagg tgacttttca atttgatttg ttaaatcgag atgaaatcag
    14461 ttgtcagctt atgtttgatt atggcaatta ccaagttagt gacaaagcaa gtttagaagc
    14521 acttcctttt gctagtcatt taaaaaaaga agaaaaaatc aatcgttctt tacttgcttt
    14581 tggcttctcg ccacaatttt actcaaaaaa gcgactaaca agtgcaaaag agctctatac
    14641 tttttttgaa gaaacagttc catgttttga gcgcttaggt aatgttgcgt tgagtacagc
    14701 tatccaagca ttacaagtga aagaaatgcc caagatcgct attagacgca atcagggatt
    14761 actagatatt tcgtttgatt tttcaactat tattgaaaat gatattgacc aagctgttac
    14821 agctcttttt caaaataatc cttattttgt gagtcaaaca ggtcaattag tggtttttga
    14881 tgatgaaact caaaaagtta gcaaaagttt acaagaatta agagcaagac agctaaaaaa
    14941 tggtcacttg caactggatg gcattagagc tctacaagta tccaaacttt ttgaaggcat
    15001 gacatctgtt catttttcaa aggaattaga agagttagcc tatcatttgc agcatcctga
    15061 aactttttca ataaaaccgt taccagttaa ggctcaaatg cgagattatc aacgcaacgg
    15121 tgttcagtgg ttatcaatgc tcaatcatta tggctttggt ggcattttag cagatgatat
    15181 gggacttgga aaaactctcc agacacttgc ttttttagct agtcacctga aatcagatag
    15241 taaagtgctc attttatctc catctagtct tatctataat tggtttgatg aatgtcaaaa
    15301 attcacacct cagctagatg ttgtggtttc ttacggactt aaacaaatca gagatcaaat
    15361 catcgaagaa ggtcatcaaa taaccataac cagctattcc tcctttagac aggattttga
    15421 gacctatcaa gcgtttcact acgattatct tatcttagat gaagctcaag tgataaaaaa
    15481 tgcacaaact aagatttcac actgtttacg tgcgtttaat acagccaatt gttttgcgct
    15541 ttcgggcact cctattgaaa ataaaatgct tgaaatttgg tccattttcc aaattgtttt
    15601 gccagggttg ctaccgacta aaaaagagtt tttaaaatta acagctgagc aagtatcacg
    15661 ctatatcaaa ccctttgtga tgcgtcgtaa aaaagaagat gtcctaccag aattacctga
    15721 tttaattgaa actaattatt caaacgagat gacagatgaa caaaaagcta tttacttagc
    15781 gcagttaagg cagatgcaag atcaaattcg aaattctagt gatgtggata ttagccgtca
    15841 aaaaattgag attttatctg gtatcactcg attgaggcaa atctgtgaca ctccaagtct
    15901 ctttatggat taccaaggcg aaagtggtaa gctagatagt ttgcgaatct tattgacaca
    15961 aattaaagaa aatggtcatc gggctttgat tttttctcaa tttagaggga tgctggactt
    16021 ggccaagcaa gagatgactg ctctaggatt gacctcttat caaatgacgg gatctacccc
    16081 tgccaatgaa cgtcaagaaa tgactagagc ctttaataac ggttccaagg atgctttttt
    16141 gatttccctc aaggcaggtg gagttggaat caaccttacg ggagcagaca cagtgatttt
    16201 aatcgattta tggtggaatc ctgctgttga aatgcaggca attagtcgcg cctaccgtat
    16261 tggtcaaaaa gaaaacgttg aagtatatcg cttaatcact cgaggaacga ttgaagaaaa
    16321 gattttagag cttcaagaaa gtaagcgcaa tttggtgact acagttttgg atggcaatga
    16381 aagccgagca agtatgtcca ttgaagaaat aaaagaaatt ttggggctaa acactcatta
    16441 atcttccttg attttagtga cattaaaaac cctttaaaga agatgacacc aaaagcacct
    16501 tatataatct cgttaaaacg cttctttttg aaatatcttt attgatatag cgatttgtca
    16561 tttgctagta atgattaaaa catttagaaa aagacaagtc atagaatcat caaggaatgc
    16621 tttgaaaaat aacagtattt aagttataat agaggatgct acaagaaggg agaatcaaaa
    16681 aaggatggta aaaaatcagt ttcctttagt tgcagatggc attgctatta gtgatccagc
    16741 aaagcaaatg gctctgtatg aaaatgaaga tctcattacc aacatccgtg gctattacca
    16801 ggacaaggag tatgatgata ttgctagaaa tgaggagttt actgctaaag caacttctcg
    16861 gcaggcccct tctagtaagc gcttttgtag caatgatgaa aaacaccatt atgtcaaaga
    16921 ggcgcgtcaa aaagcaaagc aagatctcaa ggaaaaaaga caggcttatc ttgccaaaga
    16981 aatggcctat gttcctaaac aggtttctaa aaagcaacag cctgcggatt cttctccttc
    17041 tcaaaagcaa gcaacaacag aaatgagtcg ttttacaaaa aagcttcatc aagacaacta
    17101 tattttagct gagttgccaa aagaatataa agagccaaaa aatcttcctc aacaaggaac
    17161 tacaaaaaaa aataattacg attttttaaa atccagtcaa atttacaata ataaagaaat
    17221 gagacaacaa cgagaaaaaa caattgcgca agagcttaat cttagtaggt ttgaggatct
    17281 tccttaataa atctactcag cgtatgcaga tgacaaagga gttaagatgt caaaaacgta
    17341 tcattttatc ggaattaaag ggtcaggaat gagtgccttg gcccttatgt tgcatcaaat
    17401 ggggcataag gtgcaaggta gtgatgttga aaaatactat ttcactcaaa gaggattgga
    17461 acaagcgggc atcactatct taccttttag cgaggacaat attacgccag atatggaact
    17521 tatcgttggg aatgcctttc gtgaaaacaa caaagaagtg gcctatgctt tgagacacca
    17581 gattcctttc aaacgttatc atgacttttt aggtgatttt atgaaatcat ttatcagttt
    17641 tgcagtagca ggtgctcatg gtaaaacttc tacaacgggt ttattgtcac atgtcttgaa
    17701 aaatatcaca gacacatctt atttaattgg tgatggtact gggcgtgggt ctgctaatgc
    17761 gcagtatttt gtttttgaat cagacgaata cgaacgccat tttatgcctt atcacccaga
    17821 atactctatc attactaata ttgattttga ccatcctgat tatttcacag gaattgctga
    17881 tgtgcgaaac gctttcaacg attatgctaa gcaggtcaag aaagctttgt ttgtttatgg
    17941 tgaagatgac gaactaaaaa agatcgaagc acctgcacct atttactatt atggttttga
    18001 aaagggcaat gattttatcg cttacgacat tacgcgtact acgaacggat cggactttaa
    18061 ggtaaaacac caaggagagg tcattggtca gtttcacgta cctgcttatg ggaaacataa
    18121 cattttaaac gctacagcag taattgcaaa cttgtttgta gccggcattg atatggctct
    18181 agtggcagac catcttaaaa ctttttcagg tgtgaaacgt cgttttacag agaaaattat
    18241 caatgacact attattattg atgactttgc tcatcatcca accgaaattg tggcaacaat
    18301 tgatgcggct agacaaaaat acccaagtaa agaaattgta gcaatattcc agccacatac
    18361 ttttactcgc actattgcct tattagagga ttttgcttgt gctttaaatg aagctgacag
    18421 tgtttattta gcccagattt atggttctgc acgtgaagta gataaaggtg aagttaaggt
    18481 tgaagatttg gctgctaaga ttatcaaacc atcacaagta gtaacggtgg aaaatgtctc
    18541 tcctcttctt gatcacgata atgctgttta tgtttttatg ggagctggag atattcagct
    18601 atatgaacat tcttttgagg aattacttgc aaatttaact aaaaataacc aataacatgt
    18661 cctgccttag gcaggttttc tcattctaaa ggagtttgat atgttgatta gacaggttca
    18721 aggatccgat ttggaagtta ttgctaccat tgaaagtgac aatttttcac cacaagaagc
    18781 aacgacaaga gcagttcttg aagagcgcat acggctgata ccagatactt ttttagtggc
    18841 acttatcgat caggaaattg ttggctatat cgaaggtcca gttgtcacta ctcctatttt
    18901 ggaggattct ttgttccacg gggtgactaa aaatcctaaa actggaggct atattgctat
    18961 tactagtttg tccatagcta aacattttca gcaacaaggg gttgggacag ctttgttggc
    19021 ggcactaaaa gatttagtag tagcgcagca aagaacaggt cttattttga cttgtcatga
    19081 ttacttgatt tcctattatg aaatgaatgg gtttattaac caaggaattt cagagtctca
    19141 acatggtgga accctttggt accaaatgat ttggaaactt tagaatcaca gctgattcaa
    19201 ctgttggttt gttaaaaata agtaacaaat gcatcattta attgcaatta ggatactttt
    19261 aagttataat ggcggttgat tatatttagg aggattggct ttgaccgatt ttaaggataa
    19321 agaccaacaa gatcagcagc gaagctttaa agagcagatt cttgctgagt tggaaaaagc
    19381 aaatcaaatc agaaaagaaa aagaggaaga actttttcaa aaagagttgg aagctaaaga
    19441 agcagctagg agaacggccc agctatatgc tgaatataaa agacaagatg cttttcaaaa
    19501 agagtctata gcacataaca ataagacagc taaacacttt caagctataa aagatgcggt
    19561 aatgacttca gaagcgctta aaccgacttt actttctgaa aaagaaaact catctttaaa
    19621 aacgacaaat aagagagtcg tgcaggcaaa tgagcttcaa gagactgcct ctaaagaatc
    19681 tcaagtaccg ttaactattg agaaaggtca ttcagtgaga cgaaaattaa gcaaacgcca
    19741 acagactgag cgagctgcta aaaagatttc aaccgttttg attagctcta ttattataac
    19801 ccttttggct tttactctag caggagcagg ctatgtttat agtgctttaa atcctgttga
    19861 taaaaatagt gatgcctttg ttcaagttga gattccatct gggtcaggca ataaattgat
    19921 tggtcaaatt cttcaaaaaa aaggtttaat caagaatagc actgttttta gtttttatac
    19981 aaaatttaaa aactttacaa attttcagag cgggtattac aatctgcaaa aaagtatgag
    20041 tctagaagaa attgctagtg ctttacaaga aggtggtaca gcagaaccta ccaagccatc
    20101 tcttgggaag atcttgattc cagaaggata cacgattaaa caaatagcta aagctgttga
    20161 gcataatagc aagggaaaga ccaaaaaagc taaaacacct tttaacgaga aggatttttt
    20221 ggatttagtc acggatgagg cttttattca agatatggta aaaagatatc caaaattatt
    20281 agcaactatc ccaactaaag aaaaagctat ttaccgtttg gaaggttacc ttttcccagc
    20341 aacctataac tattacaaag aaactaccat gagagaactt gtagaggaca tgctggcagc
    20401 tatggatgct actttggtac cctattatga taaaattgct gctagtggta agacagtcaa
    20461 cgaggtattg accttggcct ctttggttga aaaagaaggt tcaacagacg atgacagacg
    20521 tcaaattgca agtgtctttt ataaccgcct taatagcgga atggcactac aatctaatat
    20581 agctattttg tatgcgatgg ggaaacttgg tgagaaaaca accttggctg aggatgctac
    20641 tattgacacc accattaatt ctccttataa tatttatacc aatacaggtc tgatgcctgg
    20701 tccagttgct agctcggggg tttctgcaat tgaagcaacc ctaaatccag cctcaacgga
    20761 ttatttatac tttgtggcca atgtccatac tggtgaagtt tactatgcaa aaacatttga
    20821 agagcactct gcaaatgttg aaaaatatgt gaatagtcaa attcagtaaa cacaaacatg
    20881 gtgcttaagc accatgtttg tgtttctgat tcgttcaaaa aagaaaagag agaaaacaat
    20941 tatggcagaa aaaacgtatc cgatgaccct aacggaaaaa gaacaacttg aaaaagaact
    21001 tgaagaatta aaacttgtgc gtcgtccaga aatagtggag cgtattaaaa ttgcacgttc
    21061 gtatggagat ctttcagaga actctgaata cgatgccgca aaagatgagc aagcttttgt
    21121 tgaaggtcaa atttcaacgc ttgaaactaa gattcgctat gctgaaatca ttgacagtga
    21181 tgctgttgca aaagatgaag tcgctatcgg aaaaacagtt atcgttcaag aagttggaac
    21241 aacagataaa gatacctatc atattgtagg tgctgctgga gcagatatct tctccggaaa
    21301 aatttcaaat gaaagcccga ttgctcaagc tttgattggt aaaaaaacag gtgacaaggt
    21361 tagaattgaa tcaccagcag ctacttatga tgttgaaatc atcagcgttg aaaagacaaa
    21421 ttaatccgat gaagccatta tttgcctaac cactaagtgt tttggttgct aatggctttt
    21481 ctagtatctt caaatgaggt tgactgttag gaaggtcaag tattaatcgc attgattatg
    21541 agccaagagg actatagatc aaagatcgtt ttcgatggtt atttaatacc aaaagaaaag
    21601 accagcacct taatgtactg accccaaaaa gttggacact atattttaag tataggaagt
    21661 gtataatcta aggaaactga cactaatcat tgaccgtttt gcaagacttt attaatgatt
    21721 tcttgcttta ttttaaatga ataagtctga tacgtttcct tccttaacac tatcgactcc
    21781 ataatgaccc atcagtcaaa cggtatatct caaacgacta cggtttactc caaattcatt
    21841 ggctagttga ggccaagaaa cacctgattt ttttaatgct aaatttctag cttatcttca
    21901 tagcttaatt tcatacaaaa acaccccaat cgttagattt ttttgatatg tcccctttca
    21961 ctagatgagt ccagtaaaag ggggcatatc gttttttgtc taacttttgg agtgcagttc
    22021 ataattgtgc taggtttttt gtctttctta acgctttcgc tgttttccag cattacgatt
    22081 ggattttatt tttttaggca agttagcctg ttccatgttt tgtgaaggcg taacgtcctt
    22141 acgagaagaa gttgattggt atgctttagg cgggttttta gcgtattctt ctttaatttg
    22201 cttatgcaat cgaggtttta agaggtaggt tgtgattaac tgttgaataa tactaaagaa
    22261 accaccaact agccagtaaa gaccaacgcc agcagggagt gagaaggaca tgaagatcat
    22321 catgataggc atggtgtaca tcatcgtctt catttgctca cgttgttctt ctgaaacggc
    22381 catcattgat aaccatgatt ggaagaagta aagagcagcg ataattgctg ttaacactaa
    22441 actacggcta ccaagatcaa tgctcataaa ggtacttgtt gatactcctt tagtgtattg
    22501 agcggcaaaa tacatggcag aaaagaatgg catctggatg agaagaggta gacatccaat
    22561 acctccaaga gggttaatcc catgagcacg ctgagcagcc atcaattctg tttgggcagc
    22621 cattttttct tcctgactac ttgcctgttt gatacgtttg ttgattggtt caaaaacagg
    22681 tttcaagaag gtcattttct cagattgata acttgctttc caagattgat atagacctag
    22741 tggtaagata agtgttctaa caataatggt tacgatgatg atagccagtc cataacctag
    22801 tccagcattg tttgcaaagt aatcgataaa gtatgacatg ggttttccaa gaaattccca
    22861 aatcatacct ttgggatttc cgtgagcgtc tcttcccaca cagcctgtca aggtgaatag
    22921 aattgacagt gctagtcctg aaaataggat gcgatttaat gttagtttca atgtgtttat
    22981 cctttaattt taatatactt atctatttta cagttttttt cagaaattca caatagttct
    23041 gaaggccgat ttttaattgc ctatttgaaa gtcctgaaag tcttcaaaat tagccatggt
    23101 gacatccaca taagtgactt ttgcccattt ggatggtcct ttacgaactt cttgaataaa
    23161 tgtggctatt ttatctgaat cttttgattg ggccagaatt tcaacggtcc catcgttgtt
    23221 attccagaca cgaccataaa tacctcctat atctagagct aaagtatgag tagcgtatcg
    23281 aaaaccaaca ccttgtacac gtccagaaac aattaaacgt actttttgca taagaagggc
    23341 ctccttgctt ttattataac acatacttag caggaaatct ctttagccaa ttgtctactt
    23401 cctgtcactt ttactaaaga caagaataca aaaatgagat agttagtagt actaacagta
    23461 aaagaggttt gtctatttta aaaatagtat caaaaaaagg tcttttaggt ttaaaacatt
    23521 gaactgcccc caaattgacc atctaagaag ttgatgcttt caaatgctgt cgcgtatggt
    23581 ataataggcg catggttatt acatcaaaag caaatcaact aattaagcaa acaaaaaaat
    23641 tattgcaaaa aaagcatcga aaacagtcct atttgattga gggttggcac ctttttgaag
    23701 aagcccaaaa atcaggacaa gtttttcgcc acatctttgt tttagaggaa atggtggagc
    23761 gtctagcagg tgagcaagaa ctcgttattg ttagcccaca agtgctcaag gagttgaccg
    23821 actctcctag tccacaaggc attgttgctg aggtggagat acctaagtta gcctttccat
    23881 cagattacaa agggaaatat ttggttttag aggacgtcca agacccagga aatcttggga
    23941 ccatcattcg tacagcagac gctgcacggt ttgatggggt ctttctttct gaaaaatcag
    24001 cagatattta caaccaaaaa accctacgtt ccatgcaagg tagccatttt catttaccaa
    24061 tttggcgaac agatgtctat cagctttgtc gagagctcca agagtataaa actcccatcc
    24121 tagcaacaac tctgtcaaag aaatctgttg actataaaag cctcacacat catgaaaggt
    24181 tggctttggt gttgggaaat gaaggccagg gcattagtgc tgaaatggca gccctagcag
    24241 accaactggt tcatattaca atgcctggtc aggcggaaag tttgaatgtt gccgtagcag
    24301 caggaatcct cattttcagc ttgatttaag ctaatatgat ataataacca agtgaggtaa
    24361 ttatgttatg cattatacag aagataaaga atatatggaa cacgtcggtc atttgatcgc
    24421 tcatcctcgc tttcaaaaat tgagtcatat tgttcagcac caacattcaa cgcgtcttga
    24481 acattctatc aatgtttctt attccagtta taaattagcc aagcgatttg gttgggatgc
    24541 taaaagtaca gccagagggg gcttacttca tgatttcttt tattatgatt ggcgcgtgac
    24601 caagtttaat aagggtcatg cctgggttca tcctcgtatt gctgtacgaa atgcgaaaaa
    24661 gttaacagaa ctcaataaaa aagaagagga tatcattcta aaacacatgt ggggagcgac
    24721 cattgctttt ccgcgttaca aggaaagtta tattgtgaca atggtagata aatactgggc
    24781 tgtcaaggaa gcagtgacgc ctttgagaca aaaatggtct aatcgacggt ttcttcgccg
    24841 caagaccctc caaagtcata atcgttaagt aaaggagtta ttatgaacga tcacgttatt
    24901 tatacacaat cagatgtggg attaaatcag ttttttgcta aaatttatag tctagtggga
    24961 atgggagtgg gtttgtctgc ttttgtttct tatttgatgc tttatccatt tagagaaaat
    25021 ttaatctcta ttcttgttaa tcagccgatg atttactatg gtgctgcgat tattgaactt
    25081 attttagttt ttgtagccag tggtgcagca cgaaaaaaca caccagctgc attgcccatt
    25141 tttcttatct attctgcgct aaatggcttt accctaagct ttattattgt ggcatacgct
    25201 caaacgacag tttttcaagc atttctttct tcagcagctg tgttttttgc catgtcaatc
    25261 attggtgtca agactaaacg ggatatgtca ggtcttagaa aggctatgtt tgctgctttg
    25321 attggtgttg ttgtggctag tctgattaat ctttttattg gtagtgggat gatgagttat
    25381 gtcattagtg tgatttccgt gctaattttc tcaggtctca tcgcatcaga taatcaaatg
    25441 attaagcgcg tttatcaagc aacaaatggc caggttggtg atggctgggc tgttgctatg
    25501 gccttaagct tgtatttaga ttttattaac ttatttatca gccttcttcg tatttttgga
    25561 agaaatgatt aacattaaaa agtgagtata actactcact ttttaatgtt aacaggataa
    25621 gtaaactgta agcaagaaat tcgagataga tataagatga ggctgctaaa agacctagtg
    25681 ttaatttgta aactctttct ttttttggta tactattaag gattaaatac gaggagaata
    25741 tagatagata tgtcaacagc tatttggatt ttattactta ttgttgccct tggggtaggt
    25801 gtttttggtg ggatttttat tgcccgtaaa caaattgaaa aagaaattgg tgaacaccca
    25861 cgtttaacac cagaagctat ccgtgaaatg atgagccaga tggggcaaaa accaagtgaa
    25921 gcaaaaatcc aacaaactta tcgtaatatc atcaaacaat caaaagcagc cgtatccaaa
    25981 ggtaaaaaat aaaagctaat tgttataggg gaaacctttg tgatagttag aagtgaacag
    26041 tctttgatct caatgttcag tagcacttgt cttatcagtt agatttgtca actgttcagg
    26101 gtttaaaaag aaactcagtt ggctatttcc aactgagttt ttcatatcgt cagtaaggaa
    26161 gtaaagataa tggatacaag accaattggg tttttagatt ctggtgtggg aggtctgacc
    26221 gtcgtttgtg aattgattcg tcagctccct catgagaaga ttgtatatat tggtgattca
    26281 gcaagagccc cctatggtcc tagacccaaa aaacaaatta aggagtatac ttgggagcta
    26341 gtcaattttt tgctgactca aaatgttaaa atgattgttt ttgcatgcaa taccgcaaca
    26401 gcggtggctt gggaagaagt aaaagcagct ttagatattc ctgttttagg ggttgtctta
    26461 ccgggggcaa gcgcagctat taaatcaacg acaaaaggcc aggttggggt catcggaacc
    26521 ccaatgacag tggcttcaga catttatcgc aaaaaaatcc agctattagc accatctgtt
    26581 caagtaagga gtcttgcttg cccgaagttt gtaccgattg tggaatcaaa tgagatgtgt
    26641 tcgagtatag ctaaaaaaat agtttatgac agtctagcac cattagtcgg taaaatagat
    26701 acccttgtac taggatgtac tcactatccc ttgttacgac caattatcca aaatgttatg
    26761 gggccatctg ttaagctgat tgacagtgga gcagaatgcg tccgagatat ctctgtctta
    26821 ttaaactatt ttgacattaa cggtaactat catcaaaaag ctgtcgaaca ccgctttttt
    26881 acaacagcaa attctgagat ttttcaagaa attgcctcga tttggctgaa acaaaaaata
    26941 aatgtggagc atgtcacctt atgagtgaaa aaatttacga atacaaagat gaaaataact
    27001 ggtttatcgg aaagatgact ggtcataatt taatatcggg ctggggagtc aaacacacga
    27061 caatcaaaaa aatagatgac cttttagacg gtattgccgc aacgttagat tgggaaaatc
    27121 ctaaaggtta cgatgtttcg gtggtgcgtt atcagtctcc tttatctctc atcaccttta
    27181 ttatcgacat gataaatcaa gagacccagc gtgagattaa ggtaactccc catgcaggca
    27241 cgattttatt aatggaaaat gcgaaactgc ttgcagttta tctcccagaa ggtggagttt
    27301 caacggctac attttttgca actagtgaac agggatttgg ggatactatt ttaatcgcta
    27361 cacgaaacga gggaaaaacc aaagagttcc gcaatctgtt tgggcagcta ggttatcgtg
    27421 tggaaaatct caatgattat ccagaattgc cagaagtggc agaaactggt actacttttg
    27481 aagaaaatgc tcgcttgaaa gcagaaacga tttcacgtct cacaggaaaa atggtattag
    27541 cagacgattc aggactaaaa gttgatgcct tgggtggttt acctggggtt tggtcagctc
    27601 gtttctcagg accagatgcc acagatgcca agaataatgc caaactctta cacgaattag
    27661 ctatggtgtt tgaccaaaag aagcgctcag ctcaatttca tacgactttg gtagtggctg
    27721 ctcccaataa agatagtttg gttgtggaag ctgagtggcc aggctatatt gctacgcagc
    27781 ctaagggaga aaatggtttt gggtatgacc ctgtctttat cgtcggagag actgggcgtc
    27841 atgctgcaga attagaggct gaccaaaaga atcagttgtc gcatagaggt caagcagttc
    27901 gaaaattaat ggaggtcttt ccagcatggc aagcaaaaca atcatagtaa tgagtgactc
    27961 tcatggtgac cgagatatcg ttcaagccat taaagacaag tatcttggtc aagttgatgc
    28021 tattttccat aatggggatt ctgaattaaa cagctcagat cctatttggg ctggtattta
    28081 tgtcgttggc ggtaattgtg actatgatac aggttaccct gatcgtttag taactcaatt
    28141 aggcacagtt acgattgccc agacacatgg tcacttgtac catattaatt tcacttggga
    28201 taaactggac tactttgctc aagaagtagc agcagatatt tgtctatatg gacacctaca
    28261 ccgtccagct gcttggcaag ttgggcaaac cctctttatg aatcccggat ctgtaacaca
    28321 acctcgcgga gaaattaacg agaaattata tgctcgtgtt gaactaactg atactcaaat
    28381 taaagttgat tactttacaa gagatcataa actttactca tcactgtcta aggaatttaa
    28441 acgatgatcg caaaagaatt tgaaacgttt ttaatgagtc atttagataa ttatcttatc
    28501 ccggaacaag acttggctat ttttatcgac acacataatg cagaccatgt gatgttacta
    28561 ttggtgagca atggtttttc tagagttcct gttatcacac gggagaaaaa atatgtggga
    28621 accattagca tctcagatat tatgatgtac caatctaaaa ggcagctgac agattgggaa
    28681 atgagtcaaa cagatattgg cgagatggtt aataccaaaa ttgaaaccat tagcattact
    28741 tctagcctaa cagaaattat gcacaagtta attgatttcc cattcttacc ggttgtagat
    28801 agagcgaacc gctttgttgg gattattacg cgaaaatcta tattaaaggc tgtcaacagt
    28861 cttttacatg attttacaga tgattacacg attataaaaa aatgaagagt tatattgaac
    28921 cttttattgc tagcaaggct ttatctcaaa atagtcaaaa agcttatcga tacgatttgc
    28981 aacagttttg tcaactggtt ggtgaacgtg tcaatcaaga caaactatta ctttatcaaa
    29041 atagcatagc t
//
LOCUS       DAUGFP010000018        27893 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.018, whole genome shotgun sequence.
ACCESSION   DAUGFP010000018 DAUGFP010000000
VERSION     DAUGFP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27893
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.018"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1336
                     /gene="rocA"
                     /locus_tag="VH663_000844"
     CDS             <1..1336
                     /gene="rocA"
                     /locus_tag="VH663_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEQ8174060.1"
                     /translation="FLGFIFLDIIEIMLTLKLFSFVSAIPLRLKNIFYLSLSMVLFQV
                     VFWAFFPDHFILDVVMLAQFLFFALIALYYGKSIKAKFLMFYAFFPLVSISLVKRFIV
                     FFVMPLFGMPYSVVKHNTLLIYSITCFSIFLIYRCIQVFHFDFSTWRQYFQSHRASKL
                     LVFTNSSMALYYLCVQGIDVMSPSLSGLATTTARSIIVLFYFILFLTLLIHLERYVKQ
                     NSIEAIVQQKEYRELINYSQHLGLLYQDIQELRQLLTTVSSRLKIGIEQNDISIVRLT
                     YEGILNAEKNNAKDDRLDLTCLDKLQVEAIRHIVLAKLIEAKNKKLKVEVSIPNCIAT
                     FFLEVVDFTKLLSFLLDNAIEMSLETKQPCLSIAFLDQNHKLVIVIQSSTKQGQDDSQ
                     SVFAIPALKKRDDWQFDLRNVTTILNRYDYLTISSQIHDGILTQLIEIAKPD"
     gene            complement(1437..4142)
                     /locus_tag="VH663_000845"
     CDS             complement(1437..4142)
                     /locus_tag="VH663_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEQ8174061.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTNP
                     NFHSFHLDGQTIILDDYLKVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDKWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDDSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLADLADDLPENL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLRYAWKTLMQNHPHDSICGCSVDSVHREMMTRFEKAYEVGHYLAKEAAKQIADAI
                     DTRDFPMDSQPFVLFNTSGHSKTSVAELSLTWKTYHFGQRFPKEVYQEAQEYLARLSQ
                     SFQIIDTSGQVRPEAEILGTSIAFDYDLPKRSFREPYFAIKVRLRLPITLPAMSWKTL
                     ALKLGNETTPSETVSLYDDSNQCLENGFLKVMIQTDGRLTITDKQSGLIYQDLLRFED
                     CGDIGNEYISRQPNHDQPFYADQGTTKLNIISNTAQVAELEIQQTFAIPVSADKLLQA
                     EMEAIIDITERQSGRSQEKAELTLTTLIRMEKNNPRLQFTTSFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPNAAFWKNPSNPQHQECFVSLFDGEKGVTIGNYGLNE
                     YEILPDSNTIAITLLRSVGEMGDWGYFPTPEAQCLGQHSLSYSFESIDRQTQFASYWR
                     AQEGQVPILVEQTSHHTGSLRAEHSYVTGADDQIALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(4157..5443)
                     /locus_tag="VH663_000846"
     CDS             complement(4157..5443)
                     /locus_tag="VH663_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEQ8174062.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQMKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANGKGHQTDRTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTIFSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSIAP"
     gene            5576..6616
                     /locus_tag="VH663_000847"
     CDS             5576..6616
                     /locus_tag="VH663_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8174063.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAKKQVTSPEANKDLLLILPFASDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LSIPDQVAIVGFDNSQASRLLSPKLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            6667..8361
                     /locus_tag="VH663_000848"
     CDS             6667..8361
                     /locus_tag="VH663_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEQ8174064.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDMPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAELTVKHFFNRQLDKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFAMRGIIEGFYGTPWTREERLDCLRFIGRKRM
                     NTYMYAPKDDDYQRKQWRDLYPEDWVTYFKGLLAVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAIYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQSDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSHSWLETLTDYTEDTELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKNVSTLSAWVDELVELVNTLRKLDKPAFQQAIAPWFERVAKDQDFWQAM
                     LNQEPQLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            8386..9828
                     /locus_tag="VH663_000849"
     CDS             8386..9828
                     /locus_tag="VH663_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ8174065.1"
                     /translation="MLAFPKDFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPTLFYD
                     YVGPDTASDAYHQIESDLTLLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQVYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGR
                     IGTILNLTPAYPASQSEADTAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPEWYYDAYLMPGRRMN
                     VDKGWEIYPEAVYDIAVKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIRF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(10439..11317)
                     /locus_tag="VH663_000850"
     CDS             complement(10439..11317)
                     /locus_tag="VH663_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ8174066.1"
                     /translation="MSLLCIDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAVSAPGAVNKEKGIIEGASAIPYIHHFKIKEALEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVIGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALTEQGDPLALKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            11513..12463
                     /locus_tag="VH663_000851"
     CDS             11513..12463
                     /locus_tag="VH663_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ8174067.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIAFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLTGSG
                     ADPIKWYSNPTWWPLILLFMSVWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            12473..13399
                     /locus_tag="VH663_000852"
     CDS             12473..13399
                     /locus_tag="VH663_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ8174068.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            13480..14925
                     /locus_tag="VH663_000853"
     CDS             13480..14925
                     /locus_tag="VH663_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ8174069.1"
                     /translation="MKKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKTQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIDDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            15231..15836
                     /locus_tag="VH663_000854"
     CDS             15231..15836
                     /locus_tag="VH663_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEQ8174070.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTPQEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTITYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     TLVTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            15826..17550
                     /locus_tag="VH663_000855"
     CDS             15826..17550
                     /locus_tag="VH663_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8174071.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGFSS
                     YSISSTYKRVDAEAQMRLEETLGRLQSQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADKVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRHDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQDHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDA"
     gene            17550..19034
                     /locus_tag="VH663_000856"
     CDS             17550..19034
                     /locus_tag="VH663_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8174072.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDWLDAQQAESIRQEAYHDSLLTLWLTDELSEKEFQQLSQG
                     LPAAALTGFTVLYLDCQEWQAAIDAYFEREGQPFYLKKEEDKKVYLAVLLGKTDQAKA
                     FTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSLFYNCEVSPIRAKDKSKIEL
                     PESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTLNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            18964..22470
                     /locus_tag="VH663_000857"
     CDS             18964..22470
                     /locus_tag="VH663_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEQ8174073.1"
                     /translation="MVYRQKSSEIVTRTITKPSRATSNVKQEIDMTPDSKEQTVTDYQ
                     YHYIDQEGRKQPFNQGWRFLMADVTCAQDPSFDDSNWQVIHLPHDFSLTQPYTRNGEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFALNQSIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFMANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENANYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATLLSQLDHPQGLIGLNYANGKDYDRLHEEHSDWLLYGSET
                     VSAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNLTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSRGELVSHAQDWLKVQVEGPARLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKASQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKASTPHILITSYQHY
                     LPLPLQVCSQAFDFKKGDDIFLPQWFQVKDAQGSFVKKAFPMTWYLKEKIFAEPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLAASQKRLTYCYQYDTVQAIGA
                     ISLQNHTGTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLAVT
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            22608..23486
                     /locus_tag="VH663_000858"
     CDS             22608..23486
                     /locus_tag="VH663_000858"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ8174074.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEVRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            23721..24476
                     /locus_tag="VH663_000859"
     CDS             23721..24476
                     /locus_tag="VH663_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEQ8174075.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVAVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAQAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            24529..24867
                     /locus_tag="VH663_000860"
     CDS             24529..24867
                     /locus_tag="VH663_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ8174076.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAGIPHALYAVEAFQ
                     MLLVVVKPEV"
     gene            24869..25174
                     /locus_tag="VH663_000861"
     CDS             24869..25174
                     /locus_tag="VH663_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ8174077.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPKDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKVHHLEAKTDCKLLQVLLD"
     gene            complement(25610..26683)
                     /locus_tag="VH663_000862"
     CDS             complement(25610..26683)
                     /locus_tag="VH663_000862"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEQ8174078.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQTKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKSLTNMIGSIYPPE
                     AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKHIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(26739..27491)
                     /locus_tag="VH663_000863"
     CDS             complement(26739..27491)
                     /locus_tag="VH663_000863"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEQ8174079.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            complement(27731..27801)
                     /locus_tag="VH663_000864"
     tRNA            complement(27731..27801)
                     /locus_tag="VH663_000864"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27767..27769),aa:Thr,seq:ggt)
BASE COUNT         8078 a         5353 c         6070 g         8392 t
ORIGIN      
        1 atttttagga tttatatttt tagatattat tgaaattatg ttaacgttaa agctttttag
       61 ctttgtaagt gcaattccgc ttcgcctgaa aaatattttt tatttatcac ttagtatggt
      121 tttgtttcaa gtagtttttt gggctttctt tccagaccat ttcattcttg atgttgtgat
      181 gttggctcag tttcttttct ttgcgcttat agccctatac tatggtaaat caataaaagc
      241 taagttttta atgttttatg ccttttttcc actagtgtct attagtttgg ttaagcgatt
      301 tattgtattt tttgttatgc cattgtttgg tatgccttat tcagttgtta aacataatac
      361 actactgatt tacagcatta cttgttttag tatttttttg atttatcgct gcattcaagt
      421 ttttcatttc gatttttcaa cctggcgcca atattttcaa tcacatagag ctagtaaact
      481 attagtgttt actaactctt cgatggctct atattacttg tgtgtccaag ggatagatgt
      541 gatgtcgcct tctttatcag gacttgctac aacgactgct cgttcaatca tagtgctctt
      601 ttatttcatt ttgtttctta ctttattaat tcatttagag cgttatgtaa aacaaaactc
      661 tattgaggca attgtgcaac aaaaagaata tcgtgagctg attaattata gtcagcacct
      721 tggattgctg tatcaagata ttcaagagct taggcagcta ttaactaccg tgtccagtcg
      781 tcttaagatt ggaatcgaac aaaatgatat ctctattgtg agacttactt atgaaggtat
      841 cttgaatgct gaaaagaata atgctaaaga tgacagactt gatttaactt gtttagataa
      901 attacaagtt gaagcaatca gacatattgt tttagctaaa ttaattgagg caaaaaataa
      961 gaagcttaag gttgaggtat cgatccctaa ttgtattgca acgttttttc tagaagtagt
     1021 agatttcact aagctattgt catttttgtt agataatgct atagaaatga gtttggagac
     1081 aaagcaacct tgcttatcaa tagcatttct ggatcaaaac cataaacttg tcatagtcat
     1141 tcaaagcagt actaaacaag gacaagatga tagtcaaagc gtgtttgcta taccggcttt
     1201 gaaaaaaaga gatgactggc aatttgactt aaggaatgtc acgaccattt taaatcgtta
     1261 tgactatctc acaattagct cgcagattca tgatggcatt ttaacccagt taatagaaat
     1321 agctaagcct gactgatgtt aaaggtatta ataatcactc aaaatcaact taagagtctg
     1381 agacaatagt cccagactct ttttgaatat gttaatctat tactaatgat aatagtttat
     1441 tgtttcttcc aagctagagt taaaatttcc gctttgccaa gttggctggg tgtgctttgc
     1501 ttgctgtctt tttctaacaa attagtgacc ttggcattgt agtttggcag gcttaggcta
     1561 aagtcacaag ttttatcgtt tgagagatta tagctgcgcg tgataagggc attgtctgct
     1621 aagcgacgtt tgaaagctgt tagagcgatt tgatcgtctg caccagtgac atagctatgc
     1681 tctgctctta agcttcccgt atgatggctg gtttgttcga ctaggatagg aacttggcct
     1741 tcttgtgccc gccaatagct ggcaaattgt gtttgcctat cgatgctttc aaaactataa
     1801 gataggctat gctggccaag acactgggct tcaggtgttg ggaagtaacc ccagtccccc
     1861 atctcaccaa cagaacgtaa gagagtgatg gcaatggtgt tgctatctgg taaaatctca
     1921 tattcgttga ggccgtagtt accaatagtg accccctttt caccatcaaa gagactcaca
     1981 aagcattctt ggtgctgtgg gttacttgga ttcttccaaa aggcagcatt tggatgattt
     2041 ggacgtttga cagtttcaaa aatactatca gctaggtgat ggtctgtctt aaggtgcgtt
     2101 gggaatagga cgcgcaagcg atgattagtc atttggttat caaaacttgt ggtgaattgg
     2161 aggcgaggat tgtttttctc catgcggata agggttgtta aggttagctc agccttttct
     2221 tgtgaacgtc ctgattggcg ttctgtgatg tcaatgatag cctccatttc agcctgtaag
     2281 agcttatctg cggagacagg aatggcaaag gtttgctgga tttcaagttc agcaacttga
     2341 gcggtgttgc taatgatgtt aagcttggtg gtcccttggt ccgcatagaa aggttggtca
     2401 tgatttggtt ggcgagaaat atattcattt ccaatatcgc cacaatcttc aaaccgcaac
     2461 aggtcttgat agattagtcc agattgttta tcggtgatgg ttagacgacc atcggtttgt
     2521 atcataactt ttagaaaccc attttcaagg cactgattac tgtcatcgta gagggaaacg
     2581 gtttctgaag gagttgtttc atttcctagc tttaatgcta aggttttcca agacatggct
     2641 gggagagtta ttggtagccg taatctcact ttgatggcga aataaggttc gcggaaggat
     2701 ctcttgggca aatcgtagtc aaaagcgatg cttgtgccta aaatttctgc ttcgggtctc
     2761 acttgtccac tagtgtcaat aatttggaaa gattgggaga gtcttgccaa atattcttga
     2821 gcttcttggt aaacctcttt aggaaagcgt tgaccaaaat gatatgtttt ccaggtcagg
     2881 ctgagctcag caacacttgt tttggaatgg ccgctggtat taaataagac gaagggttgg
     2941 ctatccattg gaaaatccct ggtatcaatg gcgtcagcaa tttgcttagc agcttctttt
     3001 gctaaatagt gtccgacttc ataggctttt tcaaagcgcg tcatcatttc ccgatgaacg
     3061 ctatcaacac tacaaccaca gatagaatca tgagggtgat tttgcatgag ggttttccaa
     3121 gcgtaacgca gttggtcgtg agggtaggta cttgttacct cataagccat tgctgctaag
     3181 ggttcggtga tgttttcgag ttggcgagag actctggtat tagcctgttt gaggtaaata
     3241 cgagcagaag ccgtgttagc tagggtatac cagccatcgg tttcttgact ggtaatctct
     3301 ccttggacgg ttgaaaggtt ctctggtaaa tcatctgcga gatcagccaa gtaatcttca
     3361 aagcaggaat gcacaaattc gtagtcagga tagagttggt tggctaaggc gattgccttg
     3421 gtgacatcaa gttgtacggg ttgatgatca cacccgttca tcatcagaag gtgcttggtt
     3481 gaggcgaagc gttcggcgtc agcaagtttt ttatcccaaa aaagacgcgc ctcagcttct
     3541 gttgtcggga tctcattgcc attgctgtac cagttggcga agaggagtcc aagaatacga
     3601 ctgtcatctg gtccttgcca actgatttca gagaattggg agctgtattt ttcactggta
     3661 tctacttgat tgttaaatcc agttggacga atgccacgac caaaggcagc agcttgtagg
     3721 ccggctttta acatcagctg tggtgtttgt cccatatttc caaaggtgtc tggaaaataa
     3781 ccaagtggca cactagcgcc ccatttgtca caatcctcct tgccaatgag catattgcgc
     3841 acattggact cgctgctggt caaaaaatcg tcctgtaaga tatagaaagg tccgatacgg
     3901 agttttcccg aagcaatggc ttgtctaatc tcaggttctc gttcggggcg tacttttaaa
     3961 taatcatcta ggataatggt ttgaccatcc aaatgaaaac tatgaaaatt aggatttgtt
     4021 tgaaaaactt ctaacaggtc atctattaag ttaatcagac gcatgtggtg ttgctcgtaa
     4081 gccatgtacc actcgcgatc ccagtgactg tgtgaaataa tatgtacttt tttggttgcc
     4141 atagtggcct cactttctaa ggagcaatgc taaagccaag atagtcaaga agtagttcac
     4201 agaacatcat gttagcccaa gagaaccact cacgggtgta ttgataagga tcattgacat
     4261 cgaaaccctc gtgcattaag tgggtaccag catctgttgc cacaagcaaa tcaagcatct
     4321 cttttttact gtcttggtca agagcggtca atccttgtaa ggcaagggcg atatgccaga
     4381 tataattttc tggtgtatgg gaagaaccga tgcctgctgc tgcatttccc tgataataat
     4441 aagggttttc ttggctgaaa atggtgcggc gcgttgccag atagataggg tcatctttag
     4501 cgcaaaaacc aaggtaaggt gcagcaagga ggcttggaat attagcatca tccatgatgg
     4561 aaaactgtcc cagaccatcc acttcataag cgtaaatggt ttctcccttg ctgttttgaa
     4621 caagggcatg gtctgcaata cccgattgaa tagcttcttt cagctgcttg gcacgagtag
     4681 ctactggttc attgtgaaaa aggttgttat ataaatcttc tagataggaa agcacaacca
     4741 ctgcaaacat atttgaagga atgaggtaac catattgaca agcatcatca cttggtctaa
     4801 agcctgacca ggtcatccct gttggtgcga caggacttcc ttttccagca tgtgttaggg
     4861 tatcttcttt gcgccaagta tcgcgttcaa acaagtaagg agactgggcg tggtcttgct
     4921 caactgtcca taggtcaaga atgagttcta ccccacgatg aaaatcgtca ttaaactgat
     4981 cggtgcttcc tgtttcacga tagagcaagt aagctagctg aatagggtag cagagggaat
     5041 caatctcata tttgcgttcc caaatccaag ggttcatttg ggtacgatct gtttggtgtc
     5101 cttttccgtt agcttcttcg ttgaaggcat tggcataggg atcgatacaa atgtagcgga
     5161 attggcgttt gaccaaaccg gcaataatct ttctaatttc ttcatcttcc ttggcgagga
     5221 agaggtaagg tttcatttgc gcagtggaat cacgtaacca catggcagga atatctcctg
     5281 tcaagagaaa gctagtgcca tcttcgtagg tttttagggt tgtctctaag gtattggtaa
     5341 agctggtttt gaaattaaga gcccaatcag ggtgttggtc tccacaaagg ctttccacct
     5401 tggccataaa ttggctgacg ctagttggga ttggtcgtgt catatcatat cctccaaaaa
     5461 gatatatcat ttatcttcgg tttcattata aatgataagt taagctttgt ctataagttt
     5521 ttgaaaaggt ttacaactct tttttgacat gctatactga taacgggagt aaagaatgac
     5581 aaaattatta tacaaaaaga tatatcattt cattaaagag cagatagcat caggtcgttt
     5641 gcaaattggt gaccgcctac ctactgaaaa agaattgtca gaacagttct cggttagccg
     5701 tattacttct aagcgagcgc ttgtagaact ggagcaagaa ggcttgatta cgagaagtcg
     5761 tgggaaagga agcttcgtag ctaaaaagca agtgacgtcc cctgaagcga ataaggattt
     5821 attgttgatt cttccttttg cttctgatta tgaattggga gattatgcta agggaattat
     5881 gacctccatt gctgaaacag gttaccgtct catggttcaa ttggccagta cagtaaggtt
     5941 agacaccctg tcagactatg ctgggattat ttattatcct gaagatgtca atcacagtat
     6001 tgatttcctg ttttactgcg accatcatca cattcctcta gtcttgttgg ataaatctct
     6061 agatcttttt cagtttccgt ctgttgtagc ggataataaa ggcggtgcct gtcaattgac
     6121 tcagcacttg attgagcaag gctgtgacca gattagtttt gtggcaacag gctcttttgg
     6181 agaagtatca tccgttagag accgttattt gggttactta gcggcaatgg cagaatcgtc
     6241 tctaaaatcc cattattttc ctaagacaga gactgaaaca acagatgctt atttggaccg
     6301 tctggtgaaa accttatcgg agacgacagc cactaaaagg gggcttgtcg tggaaaacga
     6361 ttggttggct attcagctga ttcaaaaaat cacacaggta ggcttatcta ttcctgatca
     6421 agtcgctatt gtaggctttg ataatagtca agcctcccgt ttgttgagtc ccaaattaag
     6481 cacagcagct caagattttt accaaatggg gcaagaagca gctaaagtct tgttgcagat
     6541 tattcatgag ccagaccaag cagtggtgtc ttgccagtta cctgttcaag tatttatcag
     6601 agaaagtagt cactaaaaaa gacgttacta gcatgagctg gttagctcag gtaaaggaga
     6661 ccagaaatga ccatttatca cgccttaaag gattatcaag aagtcatgac aagaggagac
     6721 tatcttgtct ttgacatgcc gttgacatgc cgctttattg gccgtttttt ccgttttgaa
     6781 aatcaaaaag ccctactggc agaattggca acgtcaaagt attttcagtg gattgaagaa
     6841 ggccaagcag agttgaccgt taaacatttc tttaaccgtc agctggataa ggatgccttt
     6901 accctgaagg tttcagaaga taaggaaatt attattgaaa gccaaaacct tagaggtttt
     6961 cgttacgccc aagaagcttt gcttaaagtg atgactttca aaggggataa actttattta
     7021 ccgattgtat ctgtcaagca tagtccttct tttgctatgc gggggataat tgaaggcttt
     7081 tacggaacac cgtggacacg agaagagcgg ttagattgcc tgcgtttcat tggacgtaaa
     7141 aggatgaata cttacatgta tgctccgaaa gatgatgatt accagcgaaa acaatggcgc
     7201 gatctttatc ctgaggattg ggtgacttat ttcaaagggc tacttgcagt agccaaggaa
     7261 gaagggattg atttttggta tatgattagt ccgggacttg attttgatta taccaaggaa
     7321 gctgactatc agttgctcta tcaaaaattg caacaacttc tagcgttagg tgtttgtcat
     7381 tttgggcttt tgctggatga tattgactac caaattgtag atgctgttga gagacgattc
     7441 aaaaagactg cctatgccca ggcccacttg gcaactgagg ttcatcactt tttaaaccaa
     7501 caacatgcgg cgccagaatt ggtgatctgt ccgacagaat acgacaacca ccatgattct
     7561 atctatttgc aagagctgtc agaaaggatt cctaaagaag tcgccttttt ctggacaggc
     7621 ccaagcacgc tagcgagtca aatctcgcaa gcagatattg aaactatggc agcgatttac
     7681 caacgtccta tcatcatttg ggataatatt cctgttaatg attatcaaaa agatccagaa
     7741 cgtctctttt taaccccttt tgctaatcgc tcaccatttt tatgccaatc agactatcag
     7801 gtcaagggga ttgtttctaa ccctatgatt agttgggaat tatctaagct gaccttgaca
     7861 gacatgagcc attatctgtg ggatgctaac cgttatcagc ctagtcattc ttggctagaa
     7921 accttaacag actatacaga agatactgaa ttagccctag ctttacaggc atttgcttgg
     7981 cataatggca atcgtcatct gcacagggac cttccttttg aagtggaaga agctctcctg
     8041 gctaagaatg tatcaacgtt atcagcttgg gtagatgaat tggtagaact tgtcaatacc
     8101 ttgagaaagc tagataagcc agcgtttcaa caagctattg ctccttggtt tgaacgtgtg
     8161 gccaaagacc aagatttttg gcaggctatg ttaaatcagg agcctcagtt ggaaacactc
     8221 tatgctgact tacaagaaga taaacaccgc attggaagtg acattccaag tcgttactat
     8281 cgcatctact atcaacagca agataaacta acagctaatc aaggtcaagt tacccaagca
     8341 agacctgaag attatgctta gtcatacgag ctagaaagga atgatatgtt agcatttcca
     8401 aaagattttt ggtggggagg agccacttca ggccctcaaa gtgaggggcg ttttgctaag
     8461 cagcaccgca atctctttga ttattggtat gaagaggagc ctaccttgtt ttacgattac
     8521 gtgggacctg atacggcttc ggatgcctat catcagattg aatctgattt gaccttgtta
     8581 gcttcactag gtcacaattc ctaccgcacc tccattcagt ggacacgttt gattgatgat
     8641 tttgagcaag cgacgattaa tcctgatggc cttgcttatt ataatcgagt gattgatgct
     8701 tgcttggcca atggtattcg tcctgttatt aatttgcatc attttgacct gccaattgcc
     8761 ctctatcaag tctatggtgg ctgggaatca aagcatgtcg tggacttatt tgtcgccttt
     8821 agcaaggttt gttttgagca gtttggtgat cgggttaagg attggtttgt ccacaatgaa
     8881 cctatggtcg ttgtggaagg ctcttatcta atgcaatttc attaccctgc tatcgtggat
     8941 gggaaaaaag cagtccaggt cgcctataat ctagccctag ccacagccaa ggttattcaa
     9001 gcttacaggc gaggtccagc agaacttagt gatgggcgta ttggaaccat tctcaatctg
     9061 acgccagcct acccagctag tcaatcagaa gcggatacgg ctgctgcgca ttttgcagaa
     9121 ctttggaaca atgacttgtt tatggaagct gctgttcatg ggaaattccc agaagaattg
     9181 gttgcggtat tgaaaaaaga tggggttttg tggcagtcaa cgccagaaga attggctctt
     9241 attgctgaga atagagtgga ttatttggga ttgaattttt accaccctaa gcgcgtgaag
     9301 gctccggatg tcattccagt gattagccca tcttggagtc ctgaatggta ttatgacgcc
     9361 tacctgatgc ctggccgccg catgaacgtg gataaggggt gggaaatcta tcctgaggct
     9421 gtctatgata ttgctgtcaa gatgcgagac cattacgaca atatcccttg gttcttatct
     9481 gaaaatggtg tagggatttc aggagaagac cgttaccgtg atgaaactgg tcagattcaa
     9541 gatgattatc gcatccgatt tttgaaagaa catttgacct acctccacaa aggaatagaa
     9601 gcaggctcga attgttttgg ataccatgta tggacgccaa ttgatggctg gtcttggctc
     9661 aatgcctata aaaaccgcta tggccttgtg gaaaataata tccataccca agtgcgacga
     9721 cctaaagctt cagcttattg gtttaaaaaa gtagctactc ataaccgctt gatttcatta
     9781 gaagtaatgg aggagtttgg aggctctgct tctcacctca gtgattgaac gtgtgaacgt
     9841 ctcctttgag acaagtgaat aatgatgtta ccaatgatta aacaagggcc tttttcctca
     9901 tgttaagagg atcgaggctc ttgtatttct ttggaaggtg ttggtatttg ccgaactaga
     9961 tataagcgtt ctgtctggtt tggcatcatc tatccatctt gggtaatttg gctcttcaat
    10021 tctttagagt atcttggcta aggatgcttg tttttcatct tggtatttta ggattttctt
    10081 tcatattttt ggaccagata aaagcccctc atggtttcta gtgactgttt gttatgtcac
    10141 tgtccacgat aaggggacta tatgagtgag ttattaagtg aggctttgct tattggcgat
    10201 aactagcata gcttctaata gctcgagtat taactgatga ccaatgcctt tgacagaagc
    10261 gtcagcaaca acgacatcaa caaagggacc aggcatcagg ctgcgttcat cagcagagag
    10321 tcatttaggc agaaagccag tatcgttgta gctaatcagc aatcctttta ggatgtcttt
    10381 gtagatgtca gaggcgatta gggacaatag gggataagag ggatttagcg gtccatggtt
    10441 aggcctcctt gtgttcttgg taaaaatcaa tggcagctcc aagaagattg gcttcgtttt
    10501 taaagtgaca aatggctaat tggggtctaa ggtcggagat ttcgacggct tggtagagtt
    10561 tgtctagttc agcttcaatc gctgacagta gaaagtcagc ttgggaaaca ccacccccga
    10621 ttaaaatcag ttgaggatca aaggcgtgtt gaatgttaaa aataccgatg gctaaacttt
    10681 ggagaaagac ctgcctttct ttgagtgcta ggggatctcc ttgttctgtg agggcaagga
    10741 cagctttgcc agtataatct tttccgttat tgacgattgc actatagcgc ttggccatat
    10801 tcacaacagt gcctaattga ctaaaggttt ggtagcggtc gttcatgatc atgaaaccaa
    10861 attcgccccc aaataagtgg gcaccatggt aaatcttgcc atcaatgacc aagcttcctc
    10921 caactccagt accaataacg agcatggcta ggctactagc gccttttcct gcccctaagg
    10981 tagcttcagc taaggcggca caattggcat cattttcgat agaaataggg taatgtagcc
    11041 gttcttctaa ggcctctttt attttaaaat ggtgaatgta gggaatagcg ctggcacctt
    11101 cgataatacc tttttccttg ttaaccgctc caggagcact aacggcaata cctgagaaat
    11161 ggtaagcaga gtaacgagca acttcttggt cgagtaattg gtaaaacttt tccagactag
    11221 agggagtggg aaagctgctt tgctggctta attggccatt gtgacagagg gcaaatttaa
    11281 gggaagtgcc tcctatatca atacataaca agctcatgag gtgttctcct ttattatagt
    11341 agaatagacg atcatgtctc atcttagtat gacagattta aacaacaatg aaaaggcttt
    11401 ctaagcgatt ctatactttg tgttatcata cctataattt gttgctttca aataatgaaa
    11461 gcgcttgata tactgtaact ataacgtgat taacacaaag ggaggttggg aaatgacaag
    11521 acaaaccaac aaaaagacat ccttttggaa aaatgtgatt aaataccggg ccttattatt
    11581 aatggtatta ccaggtttca tctggttcat ctttttcttt tatattccag ttttggccaa
    11641 tgtggttgct tttaaagatt ttcattattc agcagggggc tttatggaaa gtttgaaaga
    11701 gagcccttgg gttggtttgg ctaacttcaa atacctcttt gcttctaaag atgcttggtt
    11761 gattaccaga aataccatcg cttacaatgt gattttctta ctctttaatg ttttctttgc
    11821 gattgctttt gccattatca tgagtgagtt acgaaataag cgaacggtta aggtctatca
    11881 caccatgtcc ttattgcctt atttcttatc atgggtggtt attgaatact ttgtttctgc
    11941 ctttttaaat acggataaag gtttcattaa tcagctcttg actggaagtg gagcagatcc
    12001 aatcaagtgg tattccaatc ccacttggtg gcccttgatt ttactcttta tgagtgtctg
    12061 gaaagggcta ggctacaata gcattatcta ctatgcttca gtcaaaggga tttctgatac
    12121 ttattatgaa gctgctatgg tggatggtgc aagcaaatgg caacaaatta ggaacatcac
    12181 cattccacaa ttattgccaa tgatgtctat cctattgatc attaatattg gtaatatctt
    12241 taaatctgat ttcggacttt tctacgttat tcccaaaaat tcaggcccac tttatgacgt
    12301 gaccagtgtg ttagatacct atgtctacaa tgccttgact gctacaggag atattgggat
    12361 ggcatcggca gctagccttt atcagtcagt agttgggacc agcatcttgt tagtcaccaa
    12421 tgcgattgtt cgtcgcatgg accctgatgc agccttgttt taggaggaaa acatggagaa
    12481 aaagaaaaaa gtcgaaaagg ttaatgttag aacctttgat cgaaaaacca atgctatctt
    12541 taatattctg attggtctgt ttgccatctc ttgtatcatt ccttttatct tcgtgattat
    12601 tatttccttc actgatgaaa gttacttgat taatcatggg tatagttttt ttccagatgt
    12661 ctggtcaact aaggcttacc agtatatttt tcaaggagcc atgtcccata gaatcatgag
    12721 gtcatttggg atatccgtgt ttattacagt ggtgggaacc tttattaaca cgaccatgac
    12781 atcaacctat gcttatgcga tttcaagacc ttatttccca tacagacgtt tttttactgt
    12841 ttatgcactt atcaccatgc tctttgcacc agggatggtt gctaattact tggtggtcag
    12901 caatctcctt catttgaagg atacggtttg ggctttgatt ttaccaatgg ctctgggccc
    12961 atttggcatc ttggtcatga gaacgttctt taaaaagaca gttccagata gcattattga
    13021 gtcggctcgt atggatgggg ctagtgaatg gatgattttt atgaaaattg ttctcccttt
    13081 ggctgttcca gggattgcta ccatcagttt attttctgcc ttaacttatt ggaatgattg
    13141 gtttaacgcc ttgctttatg tgcaaagtga aaacctctac ccaatgcagt acttactgat
    13201 gaaaattcag agcaacctac aggccttggc acaaaatgct ggcatgagtg cacaaatggc
    13261 agatagctta gcatcgctgc caaaagaatc agttcgtatg gctatcgtgg ttattgcaac
    13321 cttgccgatt gccttgactt acccattctt ccaaaagtac tttgtcggtg gtttgaccat
    13381 tggtggggtg aaggaatagg acttaaggaa ctggtcacaa gaccagattt tatctaatca
    13441 ttagtaacaa aagaaaacat tttttaggag gcttcactta tgaaaaagtg gcaaaaaatc
    13501 gtgtgtgtga ctggaactgt gcttgcagcg tctagtttag cagcctgcga aagcaagtca
    13561 gcatcaaagg atagtgatgt caaattattg atgtaccaag ttggtgacaa acctgataac
    13621 ttcgatgaat tgatgacaat tgctaacaag cgcatcaaag aaaaaacagg tgcaacggtt
    13681 gaccttcaat acatcggttg gggggactgg gatgataaaa tgagtaccat cattgcctct
    13741 ggtgaaaact acgacattgc ttttgccaat aattatgtgg tcaatgcaca aaaaggtgct
    13801 tttgctgatt tgacaacgtt aatgccaaaa tacgctaaga aaacgtataa aaacttagac
    13861 ccagcctata ttaaaggaaa tactattgac ggtaaactct atgccttccc agtagatgcc
    13921 aacgtttatg cccaacagat gctttctttc aataaagaac tagtggacaa atatggcctt
    13981 gacatttcaa acattaagtc ctatgcagat gctgaaaatg tcttgaaaca attccacgaa
    14041 aaagaaccaa atacagcagc ttttgctatt ggtcaagtct ttagtatgtc aggtgactat
    14101 gactacccat taaccaaaac ccaacccttt gctgtgaaaa ttgatgaagg caagccaacc
    14161 atcattaatc agtatgaaga tgagtccttt aaaaacaatc tccgcttgat gcacaaatgg
    14221 tataaagaag gtttgattcc aacagatgca gcgaccaata cagaaggtta tccccttgaa
    14281 ggaaacactt ggtttatgcg tgaagaaacc caaggtccta tggactatgg cgatactatc
    14341 ttgaccaatg ctgcaggaaa agacatcgtg tctcgtccat tgactaaacc gctaaaaacc
    14401 acatcacaag cacaaatggc aaactttgtg gtatcaagcg tatctaaaaa caaagaaaaa
    14461 gcagttgaag tcctttctct tcttaatagc gatcccgaat tgttaaatgg gcttgtttat
    14521 ggtgtcgaag gcaaagcttg ggaaaaaatt gacgacaaga aaatcaaact gctcgatggc
    14581 tatcaaccaa aaatgcacat gggtgcttgg aatactggta acaataaaat cctttacact
    14641 caagaatctg ttactgacga catgattgct aaacgtgacc aatcaatcaa ggatgctaaa
    14701 gaatctccaa tccttggttt cactgttgac acaaaagtta tcaaaacgga attgtcaaat
    14761 atttcaaatg tcatgaaccg atataaagca agtatcaata ctggtacagt ggatcctgac
    14821 gaagcccttc caaaacttct tgcagacctc aaaggtgctg gttgggacaa ggtgcaaaaa
    14881 gaagttcaaa aacaattgga cgactttgta gctaaagaca agtaaggcat tcattttctt
    14941 gattataagt tgagggctgg gaaatctcag ctcttttctt ctgatgagtg gaaaaaacgg
    15001 atcatcaaat aagtggaagt tataaaggga gaaaaattaa tgtaaataat gttactactc
    15061 ttagtaaagc ggtttccaaa agcctataaa gtgtgctata ttagaagagt ctaaactatt
    15121 aatcagagta aaactaatac cagcatctta ctgggggtgc taaaactagt ctcgtagctg
    15181 ctggtccttg aaaccgctat aagtgttaac aagaaaaaga ggactatcat atgaatggca
    15241 ttgaaaaaac ctgcttttta atttggaaaa tgatccaatt gaccctcatt tttcatctct
    15301 tgtccttagc tggactgatt gtgtttgggg tggggccagc ctggcaaacc attgtcaccc
    15361 tatttttaaa aacacctcaa gaagaaaacc actattcttt gaaaagggct gttcaacttt
    15421 ggaaatctta tttcaaagaa gctaatctaa gatttggtct ttttgccctg actttccttt
    15481 ttttaacctt taatttgcat tgggcagtac agtttccatc cttgttttgg tttaccgtgt
    15541 ctttcttgat tgtaatagcc atggtttggc taaccattac ctatctttac atggtttttt
    15601 atgcagtgag ctatgagatt aacctttgga ataatatgaa attagccttt atcagcgtct
    15661 ttttaagttt caaatccttt ttactcatgc tgagtgtttt gctgggaatt accttagtga
    15721 cttggcaata caaaggcctt tatctctttt taaccttcgg agctctagtc ttttgcttgg
    15781 actttgtcac taaggctaat cgtcgactag tagatggagt gattgatgag tgttaaaaca
    15841 ttgggaaggt atgtgactaa ccactttaag cagggttttt tgattaatcg actgatgaaa
    15901 ttatatagcc tgctaattgt aagttttttc actttagcag ctattggctt tagcagttat
    15961 tctattagta gtacttataa aagggtggat gctgaggcgc agatgcgact ggaggagact
    16021 ctaggtagat tgcagtcaca aaacgatatt accctacggg ttttagacca gttggttggc
    16081 cgttcagaag attaccataa tctctatcaa tacatgacgt taaccccaaa ccaatatttt
    16141 tcaaacgttt ttgaggactg ggaaaaagga aaagacacgg tcctattttc tgaagaggtg
    16201 aggcgtcttt ttgacttgta ccctgatgtg acttctatga ccctactgct agaggacagt
    16261 agtgcttatc tgtacgccga taaggtcatt aagacgggtc gtttgcttta tagcaagcct
    16321 gaaaaagtaa ccggaaatgt tttagttcgc tctattcgaa atccagaatc aggagatgtg
    16381 acaggtcgtc tttacctgac ctttgacaaa ccaccgactt tggtagagac acagcaggat
    16441 cactatttag ctacttttgc ttttgattac tatggccgca aactcttcca tcaaggaggt
    16501 cggagatttc ctcatttaga ggctgaggtt aaaaaggcta tcaaggcaga tcgggcagct
    16561 gatcttagta atctcagcaa ggcttatcgg atgcagtaca atcgctcggg agacctctta
    16621 gcttatgtgg ctgtcagaaa atcctattta ttagcggaag ctgtcaggac agtttttgtg
    16681 tatggccttg tttctctact cttagcttgg ctgttattgc agttgctctt tagggttttc
    16741 cgaaattata tccagcaggt ttctgaaatt acggatactg ttgaaatggt tgcagcaggc
    16801 gatttgtctt taaccatcga caacagccac atggaattgg aactgtatca tatctcagaa
    16861 gccattaatc agatgttggc cagcattaaa gcttatattg acgaagttta tgtgttagag
    16921 gtagagcaac gagatgccca aatgagagct ctgcagtctc aaatcaaccc tcatttttta
    16981 tacaacacgt tagagtatat tcggatgtac gcccttagtt gtcaacaaga agaattagca
    17041 gatgtcattt atgcctttgc gagtctgctt cgcaacaata ttagccaaga taagatgacc
    17101 accttaaaag aagaactggc tttttgtgaa aagtacattt acctttatca aatgcggtat
    17161 ccagatagct tcgcttatca tgtaaaaatt gatgagagta ttgctgactt agccattcct
    17221 aaatttgtca ttcaacctct cgttgaaaat tattttgtgc atgggattga ttatagtcgc
    17281 catgacaatg cactaagcat caaggcttta gatgagacgg atcatctctt gattcaggtg
    17341 cttgataatg gacgtggtat tagtcaagag cgcttagcag atatggaaaa aaggcttcaa
    17401 gatcaccaaa caacaggcaa tagctctatt ggtttgcaaa atgtttacct ccgtctcttt
    17461 catcattttc gagacagggt ttcttggtcc atggctaagg agccaaatgg tggctttatc
    17521 attcaaatta ggattagaaa ggatgcttga tgtataaggt actattagtt gacgatgaat
    17581 acatgatttt acaagggctc accatgatta ttgattggca ggcacttgga tttgaagtgg
    17641 ttcaaacagc aagatcaggc aaggaagcct tagcctattt gactcagtac ccagtggatg
    17701 tcatgatttc agatgttacc atgcctggaa tgacaggact ggatttaata gaagcagcta
    17761 aaacctatca tcctcagcta caaaccttga ttttatctgg ttatcaagaa ttctcctatg
    17821 tgcaaaaggc tatggaatta gaaaccaagg ggtatttgct aaaacctgtg gataaggcag
    17881 aattacaggc taaaatgaaa caatttaaag actggttgga tgcccagcag gcagaatcaa
    17941 tccggcaaga agcctatcat gacagccttt tgaccctttg gcttacagat gaattgagtg
    18001 agaaagaatt tcagcagtta agtcaaggct tgccagcggc tgctttaact ggttttacag
    18061 tgctgtattt ggactgtcaa gagtggcaag cagccatcga tgcttatttt gaacgagaag
    18121 gtcagccttt ttatctcaaa aaagaagaag ataagaaggt ttatctggct gtcctacttg
    18181 gtaaaactga tcaagccaag gcgtttacct atgatctaca aaagcgattc tcaagaaaca
    18241 tgcatcaact tattttggga gaaacagttg atgactggga aaatgtttat gaaagctaca
    18301 atcaggtgct taagagcctc ttttacaatt gtgaagtgtc accaattaga gcaaaagata
    18361 agtccaaaat tgaactacca gagtcccgtt tgcagttctt tgcttttaac aaggcgttga
    18421 tgattgggga cgaacctaca atcttgtcta aattagaagc tatttttgac gagatgaaga
    18481 ccttgaactt ttcaccagag gatgtgaagc atgtttcgtt cttgttgttt tctgacattt
    18541 accgtcaatt tcccatttta gataaaatga cttatctgtc catggtaaaa actattcatg
    18601 acagccaatc cattgatcgc attttgaggg aactaaaaaa agtgttggat gtcaccaatc
    18661 agaacaattc ccctgaaaaa cgctattcag atttagttag tgaaaccatt gattgcattc
    18721 gcaaggaata ccatcaagaa ttaaccctta aagctatcgc tgataggtta catgtcaatg
    18781 gggtttattt agggcaatgc tttaaaaatg aaaccgagcg tagttttacc cagtacttaa
    18841 atcatgttag gattcaaaaa gcgcaacagt tattgcttta taccaaccaa tccatcaatg
    18901 aaattgctta tgaaacaggt tacaatacca atcattattt tatcaagatg tttaaaaagt
    18961 tgaatggttt atcgccaaaa gagttcagag atcgttacaa ggacaattac caagccatca
    19021 agggcgacca gtaacgttaa acaggagatt gatatgacac cagatagcaa ggagcagaca
    19081 gtgactgact accaatacca ctatattgat caagaaggaa gaaagcagcc ctttaatcaa
    19141 ggctggcgtt ttcttatggc agatgtgacg tgtgctcagg acccttcttt tgatgattcg
    19201 aactggcagg tgattcattt accacatgat ttcagtctta cgcagcctta cacaagaaat
    19261 ggtgaagctg aaagtgctta taaattaggt ggtgtcggtt ggtatcggca ttatctcgtg
    19321 ttagatgagg ttttagctgg ttgtcatgtt gccattactt ttgagggttc ctatatggaa
    19381 acggaaattt atgttaatgg gcaattcatt ggcaaacact taaatggcta tcaggaattt
    19441 acctatgata tctctgatgt agtgactttt ggagctgaaa atcttctggc tgtccgggtt
    19501 gaaaacaagg ttcccagttc tcgttggtat tcaggcagtg gtctttatcg tgaagtgagt
    19561 ttgtctgttc ttccccagct tcattttgtg gcagaccagg tagcaatgac cttggctgat
    19621 actgctgtgc aggagaaagg gcaacagaaa gttgatttac gctttgccct taatcagtct
    19681 attcagactt gccactacca attgagcctc tgtttatggg aacaatcaca ttgctcaaaa
    19741 gacaagaaac tcctttatca agaaacagaa gtgcctctag ctgatttggc tttccaaaga
    19801 cagtatggtc ttacactgtc tttggaagaa ctccaattgt ggtcaccaga taatcctcac
    19861 ctctacgatt tagaactcac tctttactat caaggtcagg tcattgattg cttttgcctt
    19921 gaaactggtt ttcgtcagct gacttttatg gccaatcagg ggctttttgt gaatggcaga
    19981 gctgtcaaat taaagggagt ttgtttgcat catgaccaag gaggcctagg agcttgtgct
    20041 tacgaagatg ccctagccag acaattagtc ctgttgaaag acatgggggc taataccatt
    20101 cgtagcaccc ataacccaag tagtcccaaa cttaggcaac tagccaatcg tctcggcttt
    20161 tttgtgattg aagaagcctt tgacacctgg acctatgcca aaaatggaaa cgttaatgac
    20221 ttttcaaact atttccatca gactatcgga actgaaaatg caaactatct tcaaagagtg
    20281 aggtcacctg aaacgagctg ggcccagtat agtattgagg ccatggtctg gtctgccaag
    20341 aatgacccgt cagtgttgat gtggtcgatt ggtaatgaat tgatggaagg tttttcagcc
    20401 gatgtcagtc attacccaga gctgacaaga caaatgtgcc aatggattgc tgctattgac
    20461 acctcacgtc ccattacctt tggggacaat aaattaaaag aagctgactt ttgctggcat
    20521 gaggaagtgt cgcaaatggc aactcttctt agtcaattag accatcccca gggactcatt
    20581 gggttaaact atgctaatgg aaaagactat gataggcttc atgaggaaca ctctgattgg
    20641 ctgttgtatg gttctgaaac agtctcagcc attaccagca gggcttatta taaagagaca
    20701 aaaaaagttc ttgactccgg ttatcaccta acctcttatg atcatgctaa ggttgattgg
    20761 ggtgccttcg ctagccaggc ttggtatgat accattaccc gtgattttgt tgctggcgaa
    20821 tgcgtttgga caggctttga ttatttagga gaacctaccc cctggaataa gatcgacagc
    20881 ggtgttgtag gcctttggcc ttctcctaaa aatgcctact ttggcatttt agacacggca
    20941 ggctttccaa aagatagcta ttatttttac caaagtcagt gggcgcaggg gcagaccacc
    21001 ttgcacctgt taccagtctg gcagaaggac cagctttgtt tcgatgagca gggattggtg
    21061 gaggttgtgg tttatagcaa tgctgccagt gtgcaactga tgtttgaaga tgagcagggc
    21121 aacttaacag attatggaag gaaggcattt catacttata gcacgccaac gggacacact
    21181 tatcagcttt accaaggagc tgacgctgct aagaatcctc acgagaatct ttacctgact
    21241 tggcgagtgc cctatcaaaa agggttgcta agggcagtgg cctacgacat ttctggtaaa
    21301 agcattccaa aaaccagtgg acgttctcag gttagaacct atggttcagt ggcaaagcta
    21361 tcttggaaag cctttgaagc acctattgac gctccttggg aattgcttta tctggattta
    21421 tcactcttgg atagtcgagg tgaattggtc agccatgctc aagattggct aaaggttcaa
    21481 gtagaaggac cagcccgttt gttggctctt gataatggga atccgactga ccacacccct
    21541 tatcaggagc ctctacgcca ggcatatggg ggaaaattat tagccatttt agccctcact
    21601 ggagaggcgg gacacattaa ggtcacggca aaagcctcgc agcaacttag cagtgttttt
    21661 cagacacaag tagcctgccg tgcccccaaa gaacacttag tgacattacc tttagagaaa
    21721 tatcagttta aaaagagccg actgacaagt caacaagaaa ttggtcaatc ttgtctaaag
    21781 accacacaat tcctttctgt tttggagaac aaagacaccc agcaaaaagc aggtaaggct
    21841 agcacacctc acattcttat caccagttac cagcactatt tacctctgcc cttgcaggtt
    21901 tgttctcagg catttgattt taaaaaaggc gatgatatct tcttgcctca gtggttccaa
    21961 gtcaaagatg cccaaggttc ttttgtgaaa aaagcttttc caatgacatg gtacctaaaa
    22021 gagaaaatct ttgccgagcc tattcttgtc acgggctatg tttcttgttt tgggcaatca
    22081 ataccggttg aggcaaggct caacctagca caagctagca aaaggcggca ccaggatctc
    22141 agtttagtcg ccaagcaatc cctggccgca agtcaaaaaa ggctaactta ttgctaccaa
    22201 tatgatacgg tgcaagccat aggagccatt agcttgcaaa atcacacagg cacatctctt
    22261 agtgtttctg tggcaatcag ttatggtagt cagagctcta ttaagcaaga aaccttgatg
    22321 ctagctgata gccacttgag ccagttagaa gaggttattt tcttcgatga tattttgact
    22381 gttttgtatc tggcagtgac ccttgaagtc attgaaggac ctgacctaac tgaagacagt
    22441 attcaaattg gcctttggga tttagcatga agcatgagta aaagaagact tctttggcac
    22501 caggtctgat gaattctgta aaataaggta taataaaaaa caaataggcg tcctttatta
    22561 cgtgaaaggc gcctttacaa ggttgaaatg gaaagtgaga tttctaaatg tcagaacgtt
    22621 tatcaggcca caccctgttg gtctctttgc ttgctacccc tattcgtcat agtttgtctc
    22681 ccaaaatgca caatgaagcc tatgctaaac ttgggctgga ttatgcctac cttgcttttg
    22741 aagtagggac agagcagtta gcagatgccg tgcaagggat tcgtgcctta ggaattcgtg
    22801 gctccaatgt gtctatgcca aataaagaag ctattttacc cttattggat gacttgtccc
    22861 cagctgctga gttggtcggt gcagtcaata cggttgtcaa taaggatggt aaaggacatc
    22921 tcgtggggca tattacagac ggcataggag cgctcagagc cttggctgac gaaggagtgt
    22981 cggtaaagaa caaaatcatt accctagcag gagttggtgg ggctgggaaa gctatcgctg
    23041 tccagctcgc ttttgacggc gccaaagagg ttcgtttgtt taaccgacaa gctacacgtc
    23101 taagtagcgt tcaaaagctt gtgacaaaac tgaaccagct aacaaggact aaagtaaccc
    23161 ttcaagactt ggaagatcag actgccttta aggaggccat cagggaatct catctattta
    23221 ttgatgctac aagtgtagga atgaagcctt tagaaaatct aagtttgatt accgaccccg
    23281 agctcattcg cccagacttg gtggtctttg acatcgtgta cagcccagca gaaaccaagt
    23341 tactagcctt tgcccgtcaa catggcgccc aaaaagtcat taatgggcta gggatggtgc
    23401 tttatcaagg agcagaagct tttaaattaa tcactggtca agacatgcca gttgacgcta
    23461 ttaaacctct tttaggtgat gagtaacact ttttaagtaa gcttaattat gatatattaa
    23521 taagagatgg agcagtcatc atgggctgct aaaattcttt ttgcgagagc tgtttttaag
    23581 agcaaaaagc ttatttgaag agccttatcc ctaacgtgat acaagtcata gttcctgtga
    23641 ggtctgccag ataacctacc gtttttttag aaaagaagct cttataaaag gaaaagttca
    23701 atgaataaag gagaatagca atggtaacaa aggaagttgg gcatgacttt ttagcacgtc
    23761 taggaaaaaa acgcttaaga cctggcggtg ttgaggcgac aaactggttg attgaacaag
    23821 gacaattctc tcaggattgt cacgttttag aggttgcctg caatcgctgc acgacagcaa
    23881 tagagttagc caaaaccttc ggttgtcaga ttgatgccgt tgacttaaac cccaaggttg
    23941 tcaaagaagc acaagaaagg gttgctgttg aggggttaac ccgtcacatt acagtatccc
    24001 aagccaatgc tttatcacta ccatttccag ataaccattt tgatattgtg attaatgaag
    24061 ccatgttaac catgttgaac gcccaagcca aggagaaggc actcaatgaa tattggcgtg
    24121 tgttaaaacc aggcgggcga cttttgacac atgacgtagc ttatgaagat ccaaaaacaa
    24181 gacaagtact tgacgagtta cgtcaaacga ttcatgtcaa tgttgctccc ttgtcactaa
    24241 caggttggca agccttatat caagaacacg gtttttctca agtgacttat caccatggga
    24301 aaatgtcttt gatgacacct cgtggaatga ttaaggatga aggtcttatg aacaccctga
    24361 acattatgag acgaggcctt aagaaagaga acagacaaca gtttttaaag atgcgtcgtt
    24421 tttttaccag aacaggcaaa aatctctgct atattgctgt aaaaagtgta aaataaacct
    24481 tagaccaacc tgtccataca actaaacaga ccatagagga gttcgaagat gtcttatatc
    24541 aataatattg agcacgcgaa ggttcttgat ttgacgcaag aagtaatgat tgagcaagat
    24601 caaatgttaa gtagaacact agtccaaagg caagacctag gaataacagt tttttcctta
    24661 gataagggcc aagaaattgg gcgacactca tcaccaggag atgccatggt tactatctta
    24721 agtggcttag cagaaatcac cattgatcaa gagacctacc gggttgctga aggtcaaacc
    24781 attgtgatgc cagctgggat tcctcatgcc ttatatgcag tagaagcctt tcaaatgctt
    24841 ttagtagtag taaaaccgga ggtttaacat gattgaaaat ctctctcaac tggtgcaata
    24901 tcatgatcat caggtggcta gccgatcctt gagccgtcta ctgggattat ctcaatctat
    24961 tgttttatat gccatggccc aaggagaaag cattagtcaa gagactagtc caaaagataa
    25021 agtaatactt gttttagaag gtcagctcat tttcgattta gaggatcaaa aacaggtcct
    25081 aactcaagaa agtctcatag ccatacctgc ccaaaaagtc catcatttag aagctaaaac
    25141 cgattgtaaa ctattacaag tgcttttaga ttaagggctt attgtcaact gtattgggtg
    25201 gcctatggaa aagcaaaaga gaagatgacg ctcatcttct ctttttacgt attcaaagcg
    25261 atgacaatac gaattttaag gcttggcttt ctcatctcca gattaagccc tttagcttgg
    25321 tttagtttag aattcactaa aaaaacctta atgagtttcc tatctcctta taggtttctc
    25381 attaaggctt tttagacgaa ataattgaac gacaggcaaa agaaagatct ttttacgagt
    25441 gataccgttc tcctatagtt tcataaatcc tctttttgat gttgctgtta gttattctta
    25501 gctaaaagtt atattgttat caagacttgt cttgtagtaa catgttttag gagttccagg
    25561 ataccgttat tctcttagca agcttaacta gctagagtgg agtcactatt tataataaac
    25621 tgtcgagctt ctcgacaaat tggtgatcgt ctttgttaat ggctagggtg gtaaaaccgg
    25681 tttctgtggg taatataaag ataatatgtt gctcagaatt cttcttatct gttttaaaga
    25741 cgtcaaagag ggatgtcgca gagacatcag ctctttcaag tgttgttggt aactggtaac
    25801 gttcaaaaac ttgaaggagg ctatccaagt cctcttttgg caagagtcct ttggaaacag
    25861 ccagtttagc atcaaccacc ataccgatag caatggcttc accgtgatgg taaaaatcat
    25921 ggtggcagag cgattcaatc gcgtggccca aggtatgccc aaaatttaaa gacatgcgca
    25981 aaccattttc aaattcatct tgttcaacaa tatgtttttt attggaaatg gattggtaaa
    26041 tcagacgttc aagtccctgc ttatcggttt ctttttgaaa atcacgtaac tgctgaaaaa
    26101 gcggtctatc atgaatgaag ccgattttga gcatctcact gatgccgcag gagaattccc
    26161 gttgcggtaa ggtttccaaa aaggtggttg aaataatgat agcttctgga gggtaaatgc
    26221 tgccaatcat attggttaga cttttaaaat ggactccaac tttgcccccg atactgctat
    26281 ccacctgact aagtaaagtc gttgggattt gaatgtaggg aataccacgg taataggttg
    26341 cagcgacaaa accacccaga tccccaacaa caccaccacc aatagtaaca atcatatctt
    26401 ttttggaaaa attttcagcg atgagctggt cgtagatagc actgacacgt tccaaggatt
    26461 tggtttgacc tcccgggggg cagacatgga caaaagcgtt gtattgctga gcaaattctt
    26521 caaagagagt ctgatacaag tggtagaccg tttgatcggt gataaacaag agtttacgct
    26581 gcttgcgctc accaaggcaa tctgctaagg tttttaaaac atggtcggta aacaggatat
    26641 cataatcctt gacccgagaa tggacatgaa gtgtttgtgg cataagcact cctttcatca
    26701 gtactaggta acagagataa cgcagtgata tttagagctt aatcttgcca aagttcctgc
    26761 cccaactgtt ctaattgctt ataatcaatt tgctgggcag catctgacag cgcatggtcg
    26821 ggatcaggat gaacctccat catgatgcca ttggcaccga cagccttcgc tatcttagca
    26881 gcaggtaaga gtaaatctcg tctgcctgtc gaatgagaca cgtccacaat aatcggtaaa
    26941 tctgtctttt gttgaataat agggactgcc ataatatcaa gcatatttct agtctccaca
    27001 tcgtagccac gaacgccacg ttcgcataag ataatgttag attttccagt gtcttgtaag
    27061 taggaaaggg cgcccaaata ttcctcgata gtagccatca agcctcgttt aaacaaaata
    27121 ggcttatcga tgtgtgacaa ggtttttaaa aattcaaaat tttgcatgtt gcgggcacca
    27181 acctgaatga catcgagata gtcataggct tcttctaatt ggcgctcgga catgatttca
    27241 ctgaccgata gtaagccaaa ttcctgacaa acctcatgca agtaacgaat tccttgcaga
    27301 ccaaggcctt gaaaagaagc agcagatgtt cttggtttat aggcgcctcc tctaaaatag
    27361 ttataaccaa gttttttggc acttgaagct gctaatctaa tgtgatcata agattcaatg
    27421 gaacaaggtc caacaataaa attattctta gaacaagtct tattttgaaa gtcagacata
    27481 atgtcaatca ttcgtttgat tcctttacta attctgtatg gcgtgctcta tgggataacg
    27541 tcttatttag gaatacaaaa tctcgttgtt atccatgaag cacccttagc ctgtgaaatg
    27601 ataccataaa caagatgaaa tagcaagtaa accctagatt gtctatgttc ttagtcattt
    27661 ttattaaaaa aggcttgtta gctataaaaa atactgccct aaatcttatt agggcagtat
    27721 tttttataga tgccaccacc gagaatcgaa ctcggaacga agcattacca ttgctttatt
    27781 ataccattta actacagcgg ctaccttttt ataatactat aagttattaa ttgatgcaag
    27841 cttttcttaa aaatatttcc ttaattgttt atcatcaaaa tgagagtacc agt
//
LOCUS       DAUGFP010000019        26985 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.019, whole genome shotgun sequence.
ACCESSION   DAUGFP010000019 DAUGFP010000000
VERSION     DAUGFP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26985
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.019"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            155..421
                     /locus_tag="VH663_000865"
     CDS             155..421
                     /locus_tag="VH663_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEQ8174080.1"
                     /translation="MLRDLLKGTLRFGQLKSSIGSVSQKVLTAQLRAMEADGLVHREV
                     YAEVPPWVEYSLTETGLSLAPVIEAMSDWGQTYQEKHADLFEEG"
     gene            complement(700..1413)
                     /gene="treR"
                     /locus_tag="VH663_000866"
     CDS             complement(700..1413)
                     /gene="treR"
                     /locus_tag="VH663_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEQ8174081.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIRHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFARRRPK"
     gene            1624..3648
                     /gene="treP"
                     /locus_tag="VH663_000867"
     CDS             1624..3648
                     /gene="treP"
                     /locus_tag="VH663_000867"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEQ8174082.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QRVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYAVAGTPATEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWTIGKGISFVVLAGLTGPVKWLFSAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANAIG
                     VGGLPGFMAINVKYMIPFFICMAVAIVVPMFLTFFFRKSHIMTKTEDEAKLPETPVSE
                     APAATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGALLQPTEGVLVAP
                     CDAEVSVLFPTKHAICLVTTEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDAFTVTVEGNLPHQIKANDKLVVAMKK"
     gene            3716..5344
                     /gene="treC"
                     /locus_tag="VH663_000868"
     CDS             3716..5344
                     /gene="treC"
                     /locus_tag="VH663_000868"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEQ8174083.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMGQGNGWNALFYNNHDQPRALNRFIDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAK
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVGKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYADEVELELPPR
                     YQHGQVLISNYEKVSICEKVILKPYQTLAILAEN"
     gene            5552..7447
                     /gene="pepO"
                     /locus_tag="VH663_000869"
     CDS             5552..7447
                     /gene="pepO"
                     /locus_tag="VH663_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEQ8174084.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGDHVPDDAILANFVKFHRLVADYAKRDEVGVSPILPLIAEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPNTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYS
                     KKVIDESLSLVENAQNLAKITIAHTWSKWSKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQEPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKINGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNVTLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(7613..8653)
                     /locus_tag="VH663_000870"
     CDS             complement(7613..8653)
                     /locus_tag="VH663_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEQ8174085.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(8787..9554)
                     /gene="rpsB"
                     /locus_tag="VH663_000871"
     CDS             complement(8787..9554)
                     /gene="rpsB"
                     /locus_tag="VH663_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEQ8174086.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADTQADSIEEIVEVVEGDNA"
     gene            9858..12932
                     /locus_tag="VH663_000872"
     CDS             9858..12932
                     /locus_tag="VH663_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ8174087.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLNKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALIILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGCLESICL
                     EPFNRQESLAYINSQLGSQPVTEEEMEHLYQASQGTPFFLSEYTQALLCHEKFVPLTP
                     AIKAKLGLKLANLSSRDDVLLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMQE
                     QFDKIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKHIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTSGLQANYAIQIAAALDYLGEIAQIRYQFEKAVAY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLEIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIRQGA
                     TLSQQEKEMADHLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(12963..13949)
                     /locus_tag="VH663_000873"
     CDS             complement(12963..13949)
                     /locus_tag="VH663_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEQ8174088.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSSSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYRMGYQKVCQTIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPK
                     LAVLVLQHIAASGKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHN"
     gene            complement(14135..15676)
                     /gene="hutH"
                     /locus_tag="VH663_000874"
     CDS             complement(14135..15676)
                     /gene="hutH"
                     /locus_tag="VH663_000874"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEQ8174089.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGIACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKGVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLIEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KSENHELGKGTKVAYDLFRKEVNFIEHDKHIEIYDELNKASAVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(15687..17030)
                     /locus_tag="VH663_000875"
     CDS             complement(15687..17030)
                     /locus_tag="VH663_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEQ8174090.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRDPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNIGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFENLAKLSVIFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPKEQI"
     gene            complement(17250..17846)
                     /locus_tag="VH663_000876"
     CDS             complement(17250..17846)
                     /locus_tag="VH663_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEQ8174091.1"
                     /translation="MTTVMVKTPADYTQTDWSGGETNQLFLYPEKGDYKERTFSYRLS
                     TATVALRQTTFTRLDGYHRILMSLDKPITLSDLSHAKEISLAPFEPYAFEGDTRIVSR
                     GTCQDINLMYDDHYQGQLLPVWSDTLFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCPNQTSPAQTPVAVWAGLWYKT"
     gene            complement(17868..19541)
                     /locus_tag="VH663_000877"
     CDS             complement(17868..19541)
                     /locus_tag="VH663_000877"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ8174092.1"
                     /translation="MVLSDIEIANSVTMEPISKVANQLGIDEEALCLYGKYKAKIDAR
                     QLVALKDKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVTLAEQDNQFRFVYDADDSIETKLT
                     KIVTKVYGGKGITLTPAAKRELADLERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFI
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(19559..20185)
                     /locus_tag="VH663_000878"
     CDS             complement(19559..20185)
                     /locus_tag="VH663_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEQ8174093.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQAIITEIHAKSTALQASLLAAIDKDTEAFNLVSAVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     SAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(20196..21095)
                     /gene="ftcD"
                     /locus_tag="VH663_000879"
     CDS             complement(20196..21095)
                     /gene="ftcD"
                     /locus_tag="VH663_000879"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEQ8174094.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATTKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTA
                     ECVEISKQVAERINRELGIPIFLYEDSATRPERQNLAKVRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEIIGLAPAKALIDV
                     AEYYLQVEDFDYHKQILENHLLG"
     gene            complement(21184..23214)
                     /locus_tag="VH663_000880"
     CDS             complement(21184..23214)
                     /locus_tag="VH663_000880"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEQ8174095.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFKEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRWRPKDRIYGKPINEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFKSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIETRHSQGWVTQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTIALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVDQLFH"
     gene            23506..24771
                     /locus_tag="VH663_000881"
     CDS             23506..24771
                     /locus_tag="VH663_000881"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEQ8174096.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITDDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(24829..26361)
                     /gene="ahpF"
                     /locus_tag="VH663_000882"
     CDS             complement(24829..26361)
                     /gene="ahpF"
                     /locus_tag="VH663_000882"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEQ8174097.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHGSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     ADTANMTIIKNVATKDIVGDDHVTGLNYTERDSGEDKHLDLEGVFVQIGLIPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(26382..26942)
                     /gene="ahpC"
                     /locus_tag="VH663_000883"
     CDS             complement(26382..26942)
                     /gene="ahpC"
                     /locus_tag="VH663_000883"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEQ8174098.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
BASE COUNT         7580 a         5398 c         6048 g         7959 t
ORIGIN      
        1 catttatcag ccctatcttg tttctgttct ctctaatagt ctataatggg ttataagtta
       61 caaaaagata ctaagaggag ctgctgccta tgctgaaaaa aagaccggct tgtcccgtag
      121 aaacgacctt atcggtaata aatagaaatt gttaatatta agagacttgc taaaggggac
      181 cttgcgtttt gggcagttga agtcctcaat aggttcggtc agtcaaaagg tccttactgc
      241 ccaattaagg gctatggaag cagacggatt ggttcatcgg gaagtgtatg cagaagtgcc
      301 accttgggtg gagtattcct taacagaaac tgggttgagc ttagctcctg ttattgaagc
      361 gatgtcggat tggggccaaa cttaccaaga aaaacatgcc gatttatttg aagaaggcta
      421 gaggaaagag ttgcccgagc atactgagac cgagagaagc tgagagtttt taaaaaatga
      481 tattatcaaa ttctttacag tttgataaaa ttactttttt tctacaaaaa gacttgcgct
      541 catctcacga atatgataat ataatatcaa gtatgtttga gaaaacagaa gctcaggtca
      601 agtaagtgca cacttacaat aacacagttg ataatgggga aactcagagt caagaaaaag
      661 aaaagcaagc cttgcatttt gcaaggcttt tttctgtctt tattttggtc tgcgccgagc
      721 aaaatctaaa aatttaaatt tttccagttt atggcgactc tcggtgaatt ggaattggtt
      781 attattggaa aggtagactt tggatttgac ggaaacaaca tgttggtctg atccaaggtc
      841 aagtaaaatt ttatcacgat cggttatttg gtcaatggta acctctttga gggcaaagtc
      901 aatggcgaga tggagttcct tttccaaata ttgataaata gaatgctctg cgatttccct
      961 tgtcattatt ggcaccaaag ttttactaag gtaatcaatg tctaagacgg aagccacacc
     1021 ctcaacaacg cgttggcgcg taacacgcca gaccaagctg tttttgctaa aaccggtcaa
     1081 ttttgaaagt gtctcatcca caatcagttt atcaatggca atgacgttgg tcttagaatc
     1141 tagatttgag taagagacga gttcctgata actagttagt tctgagatag ggaacatgat
     1201 ctgatgatgt ctaatcactt gggtgccacg tccttgtttt ttgaggataa gacctgcctt
     1261 tgtgagaagt gagagagcct tgcggacggt atctcggcta gcttggtatt gttgactcaa
     1321 ttctatttca gtaggtaaaa aatccccttc tttgtaaaag tctttgttta tttttgtttc
     1381 gaggtcttta taaatacgtt catactttgt catagggtaa attacctttc cacttgtcta
     1441 tttttttaca gacaacacct tttattttac caaaataatg gcttttttgg cacaaacaac
     1501 ttgcagacaa atttgcaatc gattacaata aaggtgtcaa tcaggttcta tctaagaatt
     1561 tgaatatttc attttgacgt tgcttgttaa aagcaactag aacaaaggac taggggatgt
     1621 cacatgggaa aatttgaaca ggatgctaaa agtcttctaa ctgctattgg cggtaaagaa
     1681 aacatcaagg ctgtcacaca ctgtgcaacg cgtatgcgtt ttgttttgaa tgataataat
     1741 aaggcaaatg tcaaagagat tgaaaaaatc tctgtagtta aagggacatt taccaatgct
     1801 gggcagtttc aggtaatcat tggtaatgat gttccagttt tttataatga ctttacagct
     1861 gtttctggta ttgaaggggt gtctaaagaa gctgccaaat cagcagctaa aagtaatcaa
     1921 aatgccttac aacgggtgat gaccatgttg gctgagattt tcacacctat tattccggcg
     1981 attattgttg gggggcttat tttaggtttc cgtaatattt tggagagtgt gccttttgaa
     2041 tttcttgggc agcaggtcga aaaagggaaa ttagtttttg atgcagctgg ggatcctgtt
     2101 tggaatacga ttgtgagggt atctcctttc tggtcagggg ttaaccattt cttgtggtta
     2161 ccaggggaag ctattttcca cttcttacca gttgggatta cttggtctgt gacgcgtaag
     2221 atgggaacca ctcaaatttt agggattgtc cttggtatct gtttggtgtc gccacaatta
     2281 ttgaatgcct atgcggtggc aggaacgcct gctactgaga ttgccaaaaa ctgggtttgg
     2341 gattttggtt tctttactat taatcgtatt gggtatcagg cacaggttat tccagccctt
     2401 ttagctggtc tgtcccttgc ttatcttgaa attttctggc gtaaacggat tccagaagtg
     2461 gtttcaatga tttttgtgcc attcctttct ttgattccag ctttgatttt agcgcatacg
     2521 gtattggggc caatcggttg gactattggt aaagggattt cctttgttgt gttagctgga
     2581 ttgactggtc ctgttaaatg gctattcagt gctatctttg gtgccttgta tgctccgcta
     2641 gttattactg gtttacatca catgacaaat gccattgata cccaattaat tgctgatact
     2701 gcaactcgta caactggttt gtggccaatg attgctcttt caaatatcgc tcaagggtca
     2761 gccgtttttg cttactattt aatgaatcgt catgaagaac gtgaggctga aatatcgctt
     2821 cctgcagcaa tttctgctta ccttggggta actgagcctg ctttatttgg ggttaatgtt
     2881 aaatacgttt atccctttgt agccggaatg attggctcag gtattgcggg tctcttatca
     2941 acaaccttta atgttcaggc aaatgctatt ggtgttggtg gcttaccagg tttcatggct
     3001 atcaatgtga agtacatgat tccattcttc atctgtatgg cagtagccat tgtggtaccg
     3061 atgtttttaa ccttcttttt ccgtaaatca catatcatga ctaagacaga agatgaggct
     3121 aaactacctg agacacccgt ttcggaggct cctgcagcaa ctgctccaca taagactatg
     3181 caagggacag ttatcacttt aacaagccct ttaacgggtg aagttaaagc gttgtctgaa
     3241 gctgttgatc ctgtctttgc acagggagtt atgggccaag gtgctcttct tcaaccgaca
     3301 gaaggggtgt tagtagcgcc ttgtgatgct gaagtatcgg tcttgttccc aactaaacac
     3361 gctatttgtt tggtgacgac tgaaggtttg gaattattga tgcatattgg catggatacg
     3421 gttaacttag atggtcaagg atttgaagct ttggtgaagc aaggtgatca ggttaaggct
     3481 ggacaaacat tgattcaatt tgatatagca gctatttctg aagctggata cgccactgaa
     3541 acgcctcttg tggtgactaa tcaagatgct tttacggtaa ctgttgaagg taacttaccg
     3601 catcagatta aggctaatga taagttagta gtagcgatga aaaagtaggt ttactaggga
     3661 gaaaacgttc aggaactgag atcagttcct gaaactattt tagggaggaa aactcatgac
     3721 aattgataaa aagaaagtcg tctatcaaat ttacccaaaa tcctataagg acactactgg
     3781 aaatggtgtg ggagacttgc gagggatcat tgataaattg ccttacttac aagaactagg
     3841 aatagatatg atttggttga accctttcta ccctagtcca caacgagaca atggctatga
     3901 tgtttcagat tatacggcgg tcaaccctga ttttgggacg atggctgatt ttgaagattt
     3961 ggtaaaagct gctaaggagc atcagattga gttaatgttg gatatggttt tgaatcactg
     4021 ttccacagac cacgagtggt tccaaaaagc tttagcagga gacccttatt atcaggattt
     4081 ctttatcttg agagatcagc cgactgattg ggtttccaaa tttggtggga atgcttgggc
     4141 gccttttgga gatacaggca agtactactt acacttgttt gatgtgacac aggctgactt
     4201 gaattggcgg aacccacatg ttcgtgagga attggctaaa gtggttaatt tttggcgaga
     4261 taaaggagtg aagggattcc ggtttgatgt gattaacctg attgggaaag atgaagagct
     4321 ggtggattgt ccggtcaatg atggtaagcc agcttatacg gatcgtccta ttactcacac
     4381 ttatcttcat gatctcaatc aagccagttt tggtcaggat gattcgttta tgacagtagg
     4441 ggaaatgtct gccacgacta ttgacaactg tcttttatac acggctcccg aacgtgagga
     4501 gctatccatg gcttttaatt tccaccatct aaaagttgat tatgagaatg gtcagaaatg
     4561 gactattatg gcttttgatt ttgcagcgct gcgagactta ttccatgctt ggggtgaagg
     4621 catgggtcag ggcaatgggt ggaatgcctt gttctacaat aaccatgatc aaccacgtgc
     4681 cctgaatcgt tttattgatg taacacattt ccgaaacgaa ggtgcgacga tgttagcggc
     4741 ttccatccat ttgtcacgag gaacgcctta catttatatg ggtgaggaga ttggcatgct
     4801 tgatccagat tttgatagta tggatgatta tgtggatgtg gaaagtctca atgcttactc
     4861 aagcttatta gtctcaggta aaagtgcgaa agaagccttt gccattatca aggctaagtc
     4921 aagggacaat gccagaacac caatgcaatg ggatgctagt gaacatgctg gctttacgac
     4981 tggtaagcct tggttagagg ttggcaaatc ttatcgagac atcaatgtcg aaacagaaaa
     5041 agaaggacgt atttttcctt tctaccaacg cttgattgct ttgcgtaagg aactccctat
     5101 tattgctgaa ggggactatc gggctgcttt taaagatagt caggctgtct acgcctttga
     5161 acgccattta ggtgaccagt gtttgcttgt tctcaatcat ttctatgctg atgaggtcga
     5221 actggaatta cctccacgtt atcaacatgg acaggtctta atcagcaact atgagaaagt
     5281 ttctatttgt gaaaaagtga tactgaaacc ttatcagaca cttgctatct tagctgaaaa
     5341 ctaactaaaa gcctgaggtt aggcctcagg cttttggtga ttgtcacgaa aaaagaagtt
     5401 ttctcatata tttattttta aactcttatt ttttctaaat aaaaacaatt gagataagtt
     5461 attttagcta aattttggta caatggaaaa gattataagt agagtttaag ataaagattc
     5521 tacaaattta aaatgatata aggagatacg aatgacaact tatcaagatg atttttacca
     5581 ggctgtgaat ggaaagtggg ctgaaacagc agttattcca gacgacaagc ctcgaacagg
     5641 tggattttca gacttagcag atgaaattga ggcattaatg ctagacacta cagatgcttg
     5701 gttagcaggg gatcatgtcc ctgatgatgc aattttagca aactttgtga aattccatcg
     5761 tttggtggct gactacgcta aacgtgatga agttggagta agtccaattc ttcctttaat
     5821 tgcagaatat cagtccttaa aatctttttc agaatttgtt gctaatatag cgaaatatga
     5881 attggcaggt ttaccaaatg aatttccctt tagtgtggca ccagacttta tgaatgctca
     5941 gctgaatgtg ctttgggcag aagcaccaag cattcttttg ccaaatacaa catactacga
     6001 agaaggcaac gaaaaagctg aggaattgcg tggtatttgg cgtcagtcgc aagaaaagct
     6061 tttacctcaa tttggttttt caacagaaga aatcaaggat cttttggata aggtaattga
     6121 gctagataag caattggcca agtatgtctt gtctcgtgag gaaggctcag aatatgccaa
     6181 attataccat ccttatgtgt gggctgattt taaaaaacta gctccagagc ttcctttgga
     6241 tagcattttt gaaaagattt taggccaagt acctgacaag gttattgttc ctgaggaacg
     6301 tttttggaca gaatttgcag ccacttatta ctctgaagcc aattgggatt tactcaaggc
     6361 taatttgatt gtggacgcgg ctaatgctta caatgcttac ttgacggatg atattcgtgt
     6421 ggaatcaggc gcttattcgc gcgctctttc aggaacacct caagctatgg acaaacaaaa
     6481 agcagccttt tatttggcgc aaggaccgtt tagccaagcg cttggcttgt ggtatgctgg
     6541 tcaaaaattc tccccagaag ctaaggctga tgtggaaagt aaggtagcac gcatgattga
     6601 ggtttataag tctcgtctag aaacggctga ttggttagca ccagccacac gtgaaaaagc
     6661 cattactaaa ttaaatgtta tcacgccaca tattggttac ccagaaaaat taccagaaac
     6721 ctattctaaa aaagtgattg atgagagctt gtcattggtt gaaaatgcac agaacttggc
     6781 taaaatcacg attgctcaca cttggagtaa atggagcaag ccagttgacc gtagtgagtg
     6841 gcatatgcca gcccacttag tcaatgctta ttatgatcct cagcaaaatc agattgtctt
     6901 tcctgcggca atcttacaag agccatttta ttctttagat cagagttctt cagccaatta
     6961 tggaggaatt ggtgcagtca ttgcgcatga gatttcacat gcctttgaca ccaatggtgc
     7021 ttcctttgat gaacatggta gcctaaatga ttggtggaca caagaagact atgctgcctt
     7081 taaagagcgt acagataaga ttgtggcgca gtttgacggc ttagagtcac atggtgctaa
     7141 gatcaatggt aaattgacag tttcagaaaa cgtcgctgat ctaggtgggg tagcttgtgc
     7201 tttagaagcg gcacaatcag aagaagactt ctcagcccgt gacttcttta ttaactttgc
     7261 gacgatttgg cgtatgaagg cccgtgagga atacatgcaa atgctagcta gcattgatgt
     7321 tcatgccccg ggtgaactgc gaacaaatgt caccttaacc aactttgatg ctttccatga
     7381 gacctttgat attaaagaag gcgatgctat gtggagagct ccgaaagacc gtgtgattat
     7441 ttggtaatta ggtagttgat aaaccttcaa atgcttgtga ttataaaaat gactgattct
     7501 gtgaaacaca ggatcagttt tttgcttcct tattggtgtc cgttttgaca caacaaaaag
     7561 acaaccctca ggaaagaagg tcgccttttt tagacttatc agaaataagt gattagttgt
     7621 taagggcagc agccatagta gctgcaactt ctgattcaaa gtcgttagct tttttctcga
     7681 taccttcacc aacttcaaaa cgtgcaaaag caatcgcttt agcgtttact gagtcaaggt
     7741 aagcttcaac agttttgctg tcgtccatga tgtaaacttg tgcaagaaga gtgtaagctt
     7801 ggtcaacttt agtgttgtca agcatgaagc ggtccatttt acctgggatg attttgtccc
     7861 agatcttttc tggtttgcct tcagcagcaa gttcagcttt gatgtctgct tcagcagcag
     7921 taatcacgtc gtctgataat tgagcttttg aaccgtattt caagaatgga agtgctggtt
     7981 tgtcaaccat tgcacgcgat tcgttgtcaa gttcgatagc gtggttcaat tgagcaagtt
     8041 catctttgat gaattgtgca tcaagctcag tgtatgaaag aacagttggt ttcattgcag
     8101 cgatatgcat tgatacttgt ttagcaagag catcgtcgcc gccttcgaca actgagataa
     8161 ccccgatacg gccgccatta tgttggtaag caccgaagtg ttgctcatct gttttttcaa
     8221 tcaaagcaaa acgacggaat gagatttttt ctccgatggt agcagttgca ttaacgtaag
     8281 cttcagcaag agtttcacct gaaggcataa caagtgcaag tgcttcatcg ttgttagctg
     8341 gtttgccttc agcgataact ttagcagttg cgtttaccaa ttcaacgaat tgagcatttt
     8401 tagcaacaaa gtctgtttca gcgttaactt caacaacagc agcaacgtta ccatgaacgt
     8461 aaacgcctgt taaaccttca gcggcaacac ggtcagcttt tttagctgct ttagccattc
     8521 ctttttcacg aagaagttca acggctttgt ccatgtcacc atctgtttca acaagtgctt
     8581 ttttagcgtc cataacgcca gcaccagatt tttcacgtaa ttcttttaca agcttagctg
     8641 taatttctgc catgtgtagt atcctccaaa tgatatttgt tttggtttca aaaaacgaga
     8701 cagagcggtt tgctttctgc cccgtttggt tttcgattgt taaagacaaa caaagtttgc
     8761 cataagagtt aggaactctg tgctaattaa gcgttgtcgc cttcgacaac ttcaacgatt
     8821 tcttcgattg aatctgcttg agtgtcagct tcaaaagcaa cgtctgcatc ttcaccttga
     8881 cggccttcga taatagcatc agctaattta gcagtgatta atttaacggc gcggatagcg
     8941 tcatcgttag ctggaatgat gatatcgata tcatctggat cagcgtttgt atcaaccatc
     9001 gctacgactg ggataccaag ttttttagct tctttaacag caatttgttc tttatgtggg
     9061 tcaacaacat acatcacgtc tgggatacga ggcatatctt cgataccgcc caagaatttt
     9121 tcaagacgag cgcgttgttt gttaagaagt gcaacttctt tcttaggaag aacgtcaaag
     9181 gttccttctt cttccatacg tttgatttct ttcaaacgag cgatacgttt ttggatagtt
     9241 ccccagttgg taagcgttcc acccaaccaa cggtggttga tgaagtattg gcctgcacgc
     9301 gttgcttcgt cagcaactgc ttcagcagct tgttttttag taccaacgaa caagatgaca
     9361 gcatcgtttg ctgcagcatc acgaacaaat tcgtaagctt ggtcagctaa ttttacagtt
     9421 tgttgaagat cgataacgtg gataccgtta cgctctgtga agatgtattt agccatctta
     9481 gggttccagc gacgagtttg gtgaccaaag tgaacaccag cctcaagaag ttgtttcatt
     9541 gaaattactg ccatgagtag tttctccttt ataaaatgtt ttttcctctt ccagacttcg
     9601 acttgcaggc taacctagcg gcaacaagcc cacaattggt ctagaatgag tatttgccgt
     9661 ctacacgacc ctactatatt atcaaaaaaa gaggttaaaa gcaaggagaa atgctatgaa
     9721 ttcccttatt ttcgagatga ctagcttctt tgtttattgg taagtgttaa gacaagcttt
     9781 tgctcatttt gggtgacact tgccaaatcg ttttatttaa gatatagtga acttgactaa
     9841 tttttggagg cgttttcatg acgaagggta ttcgatttca attgcttggc agtccccaca
     9901 tttttcttga tgacaaagaa caattttttg cctttgccaa agcaaatgct ctcctttatt
     9961 acttagtggt taatggatct gttagccgcg aggtggcggc gagtctactt tgggaaaata
    10021 agaacacgca aacagcgaaa aagaatttac gaaacgctat ctaccaggtc aataaagtct
    10081 tgcaagctga tgtgattatt tgtcccaatc gcaacctgct ggttttgaat aaaacacttg
    10141 atatcaaaac agatattaac ctttttctgg ctgatccgct agctcactta gaactctacc
    10201 aaggggagtt tttgcagggc ttttacctta aaagtggtga ggaatttgat ctttgggttt
    10261 caaaaatgcg tatgcagtat gaacaggtat acctaaaagc gtgttaccaa aaaattgaag
    10321 aaaagctctc tttagatgcc attgaagatg tcgaggaaca tttaaaacag cttattgaac
    10381 gtgatgaatt tgaagagaaa aattatcagc tcttgatgag gctttatcag caggggaatt
    10441 gtcctggtaa ggtcatcgaa acttattatc agttggctaa tgtgttggat aaggagctag
    10501 ggattcagcc aagtttgcaa agccaacaga tttatcagga agtggtcgct aaagatcgta
    10561 atgaacgcaa aatcaagcat tttttgcgca acagtaatca ttttttgggg cggattgatg
    10621 agattaagca gctggaaaac tattttgcca actgtttggc ctgccaagag gtaggagctc
    10681 tgctcttaat tggtgataca gggattggga aacgaaccct agccagacag gtcttagcca
    10741 atcaaaccca aacgtttcaa attgtcacag ctaaatgttt tcgtgaagag gctatggatt
    10801 ccttattgcc ttggcgtaat atcttagatg gcctgggaga tttggtgatt caaaaccgct
    10861 tattgaccac caaagcttgg aaggctgctc ttaaacgctg ttttcctgtg gcaaccattt
    10921 ttcaagaaga taacaaccaa ccctttatca aggaccacac cagcttgttg gtttccttta
    10981 ttgttgatat tttacaacat ttggcagaaa tcaaggcgct gataatcctt attgaggatt
    11041 gtcattggat ggatgaggac agtttgacct tgttgcaacg ggtcatgaat caattggtcc
    11101 actatccgat tgcttttgtc ttgaccaagc atctaggaac gactcctgag ctcggtcttt
    11161 gtttgaatgc tttgatgagt caggggtgtt tagaaagcat ttgtttggag ccttttaatc
    11221 ggcaagaaag tttggcttat attaacagtc aactgggtag tcagccagtg acggaagaag
    11281 aaatggagca cctctatcaa gccagccaag gcaccccttt ctttttgtca gaatacactc
    11341 aggccctact gtgtcacgaa aaatttgtgc ctttaacgcc tgccattaag gctaagttgg
    11401 gtcttaaact agctaatcta agtagtcgtg atgacgtatt gttaaactat ttgtcttgtt
    11461 gtcggaggcc tatccctcta aataccctcg ctcaattgat gttactgcct ttagaagaag
    11521 tgatcgagat ggtggataat ctggggcact actatatttt ggtggaagaa agcgtaggag
    11581 aggaggtgtt gatttcattt cgtcagcgaa ttattcaact ctatagttat gaccgtttat
    11641 ctttgtcaaa aaggcgttta ttgcacggac aaattgccaa acggttagaa gatttactgc
    11701 ctattttgac accaagccct cacttattag atgacattgc ttaccattat caggaatcac
    11761 ggcaggtgat taaggctttg gaatacaacc ttaactatct agatgctacc ttgccatttc
    11821 agcatgagct gtttcccatt tattcaaaga gtattggctc cttggaaaag tcagatcgtg
    11881 accatcaacg cttaatgcaa gagcagtttg ataagattcg gcaaagcatt gcggatttgg
    11941 aactgaccta tgataataac cgtgatttcc agcagttgct tattcgtttt tcttaccttg
    12001 aaggccgtta tgatattcga acagggaggt atcaagaagg gattaaacac attcaaaagg
    12061 tcatcgcttt ggcaaccgag ctaaaacagc cctccttctt gctagaaggc tatcgccagt
    12121 taattcatta ctgtatccaa gtggaaaaca agccagaaat gcgttattac actagtcttt
    12181 cattggaggc agcggttgct gctaatcatt ttgaggccat tgcgattagt ttgcggttaa
    12241 acggtcttta tcatttgatt attggggagt taaatgaggc agagcgtctg cttcaacagt
    12301 cgattgactt ctttaaggtg acgtcaggct tgcaagctaa ctatgcgatt cagattgctg
    12361 ctgccttaga ttatttgggg gagatcgccc agattcgcta tcagtttgaa aaggccgttg
    12421 cttaccaaaa acaagccatt gccttgaccg aaaacaagcc agctgagtta tcagtgagta
    12481 ttttttatat tggattaggg atttcctact tttatttagc tgattttgaa caagctgagc
    12541 aaatcttgag tttggctaag gaagctttgg tcaatcatag ttatccttgg aaagaaacgc
    12601 aacttgaaat ttacctggcc atgattcaat ggaaaaaggg taattatcag ccagccttga
    12661 ctctacttga ttacagagaa accttgatgt ctaggtatcg caatccgcga gataagggct
    12721 tggttttcta tttgatggct gtggtcaagt accaattgat acgtcaagga gctaccctaa
    12781 gtcagcagga aaaggaaatg gcagaccacc tgttgtcaga atcctttgag tattattatg
    12841 agattgctag tactaacctc aacccttacc gagattgtca tttggtgagt gagttgaacg
    12901 atttgcgtca acaattatct gctaaaagct aaggaaaacc ctctgattca catcagaggg
    12961 ttttagttat gttgtgccat aatctgtacg aggtaaaaga taaaggtggc tgctaaattg
    13021 gcggtgtggt tatcaatatc gtgtggtggt gaaacctcaa ccacatcaaa gccaactaat
    13081 tttccgctag ctgcaatgtg ttgcaaaacc aagacggcaa gttttgggtc aacaccgagg
    13141 gattggatag cactaacccc aggtgcagca ccaactgaga agcagtccat gtcaatggtc
    13201 agatagactc tttcttgccc ttctaagaaa cgatcaatgg tttggcaaac cttttgatag
    13261 cccatgcgat aaatatcttg cccagtcaaa aattgaatgc ccttggattt tgccacaaag
    13321 tcaaatagga aaaggttgtt attatgttct tgaatgccta aaacgaagta tttaaagagg
    13381 cgtttgtcag caaccgcatc gtcaaacatt tgtcgaaagc ctgtccctga attagggcct
    13441 gtttggtcgt agggtcttaa atcaaagtgg gcgtccatat tgatgacagc taggtcatca
    13501 cttgaggata ggctttggcg taggcctaag tagtggccgt aggcggtttc atggccacct
    13561 cctaagacga tgggtttgag attgaggtca cacatccgtt tgatggcttt gcttaaactg
    13621 ttttgcagtt gttccaaaga gcgattgggg ccgtcaatgt tacccacatc gtaaaccata
    13681 acttggtttc ctaaatgcca agggaatttg gcgagctggg tacgtatagc agctggactt
    13741 tcgaccgctc cgacacgacc attattgata taaactccct tatcactttt aaagccaatc
    13801 aaggcaaaat gtgtcccttc aaatggggtt agtgagctgt cgttaaggtc aaggaaagtc
    13861 atgaccatgc cccatttggc agtatataag tcatcgtcga tcccaccatg ataatagcta
    13921 gtggtggatg ggtagtaatc ttctagcatg cgcgtcatct cctttaggat tatgatagca
    13981 aaataaaact tacagtcaac actttcttgg cctttgtcac tgctttttag gaatagcaga
    14041 gcaataaaat cgtctgcttt tggtcccggt tagttagtct aaagaggcca tgacctctgc
    14101 cacagcccct ctagttaaag aagtacctta ttcactaaaa ttggatactc aactcaactg
    14161 cctgctcaac cgcttcgagg aagcttgggt cttcaatgac tgctgatgcc ttgtttagtt
    14221 catcgtagat ttcgatgtgt ttatcgtgtt cgataaagtt gacttccttg cggaagagat
    14281 cgtaggcaac cttagtccct ttacctagtt catgattttc agatttgaga tcaagagctt
    14341 ggcaggctgc catgatttca gtagccacga tgcggcgaga atttttaagg atttcaaagg
    14401 ccttgcgagc agcggtggtg cccatgctga caaagtcttc ttggttctca caagatggga
    14461 tagaatccac gctggctgga tgtgccaaaa ccttgttttc agaagcaaga gaggcacagg
    14521 cgtactgggt aatcataaag cctgaattga gacctgggta tttgacaagg aaggatggca
    14581 atttgctgag ctggctattg accaaacgtt ccacgcggcg ttcagaaaca tttccaattt
    14641 cagaaatggc gatgcctaag aagtcaaatg gctgtgccat tggttctccg tggaagttac
    14701 ctcctgaaat aacgtgacca tctttacaga taattggatt atctgtgaca gaattgattt
    14761 caatgtcaac tttggatttg acataggcga tactgtcttt gctagcccca tgaatttgag
    14821 gcatgcagcg caaggtgtaa gggtcttgca cacgagattg agtagctacg gttgtgtttt
    14881 ggctgccttc aatgaggttt cggatattgc gggctgttgc caactggccg ctttgtgggc
    14941 gaatggtgtg aaggttttct tcaaatggac tagtaatccc gttatgcact tcaagggata
    15001 gggcaccagc taggtctgac agtttcaata attgaatggc gtcataggtt gcaagggcac
    15061 cgattgcagt gagaacagtt gttccgttga taagggcgag tccttcttta gcggctaaag
    15121 aaatcttgtc aataccagct ttatcaagag cttcttgacc agaaagaagc tctcctttgt
    15181 agtaagcttt ccctagacca agcataggaa gcaccatgtg agcaagcggc gccaaatctc
    15241 ctgaagcccc tagagaccct ttttcaggaa tgtaaggatg aaccccttta ttgagcagtt
    15301 ccaacagctt ttcaatggtg gatagacgaa taccagaata ccctttgacg agagagttga
    15361 tacggatcag catgatagcg cgcacggcat cttctggcaa tgggtcacca aatccactag
    15421 catgggtacg aatcaaattt tcttggagtt gaactgtatc ttctggtgag atgctcacgt
    15481 tacaaaggga tccaaaaccg gtggtcaccc cgtaaaccac ccgtttttca ctgacaatgt
    15541 catcaacaat tttacgagag gcattcacag cttcaatagc actatcatca atgcgacagg
    15601 ctatgccttg gcgagcaatc gcaatcacgt cttcgatagt aaggctttcg ccatctaaat
    15661 taatcactct tgtcatagtt tttctcctag atttgttcct tagggggcat ttggtccatt
    15721 tggtggttac gtccgtgcat aaggtagtac acactacttg caatgatgac accaactgca
    15781 ccataaacaa agttcattgg gttgtcaccc caaatcataa ctaaactaac gataactgca
    15841 agaattggga taataggacc aaatggtact cggaagatca cattagcatc gggatcgtcc
    15901 ttccgtaatt tcataacggc tagtgctgtt ggaatgtatt ggaagaagcg gaaaataaca
    15961 ctgagttttg ctaagttttc aaatgagcca gtgagtagga gtacaatagc aattgctcct
    16021 gaaaccaaga tagcaacgag tggtgcgcca ttttgatttt gcttggcaat agcagctggt
    16081 aagagccctt catcagcaat ggcagcaccg taacgtggca ccatgatgga ttctcctatg
    16141 ttaagacctg taatggaaat taaagcaccg attgagacca tccaagcgcc agcagggcct
    16201 atcattttaa caaaggcatc ttgaacaggt gcattggtca tcatgatttg agagccaagc
    16261 atggcaatgg ttccaccgat aatcagcata tataagacag agacgatact gattgatcct
    16321 aaaagagcac gcggtacatt tttttcaggg tcacgcattt ccccagcgac aatagagagg
    16381 gtttcaaaac cgataaagcc atagaagata taaacagcag tattggagat agcccctaag
    16441 aggttagtcc ctggttccaa ctgaacaaat ggggtaaagt taggaagccc gtttttgatg
    16501 aagaaaaggg tacaggcaca aaaggcaacg attggaatta acttagcaat ggtggctgtg
    16561 atggtgacaa ttttagaggt tttcaaacca gcgatattca tcaaagatag taagatgatt
    16621 aacccaatgc taagtggaat gtgccaacct tcaaaggcag ggaaagtaat gataaacatt
    16681 ctggcaaacc cagctgccat ggctgcccaa gcgaagatgg taacggtcca accaaggaag
    16741 ccaacgttaa agccaataaa atcaccaaaa gctcgtttgg aatattggaa ggctccgccg
    16801 tttttcccaa aataaccaga gacttctgca aaacaaactg ccaacatgat ggtcaaaata
    16861 gctgttccaa acataacagc gatggatgca gggccaaggc ccttgtaaat ggccctaggt
    16921 aagagaaaaa taccagagcc gataacggca ttgatcccgt aaagagtggc accgctcaag
    16981 ctaaactttg cctgttctct ttcttgttcg ttcatgtgat ttgacgccat aataatgctc
    17041 tcctttttgt ttgcaaattg cgttcaaata taatggataa ggtcgctagg aaatgaggag
    17101 atagtcggtt taggagggtc cttcttgcac ctaacgatag tgatgagcta ttggttagcg
    17161 atgagaaata ggtagccttt tgtgatgatg tgtagtgatt tcattggaga agaataggca
    17221 tgagtagtgt gatgcctatc tttatcccac taagtcttat accataagcc tgcccaaaca
    17281 gctactggag tttgggcggg gcttgtttga ttggggcaaa cttttaggtc tgaaaggctg
    17341 gcctcctgct cgattaccaa caagtgattg gcttcaagct cttgattcgc ctgattggtc
    17401 acagaataag tcagtttgct tagggcataa agcagttggc agtccgcttt gggtgcctca
    17461 aatagtgtgt cagaccagac gggtaataat tgcccttggt aatgatcatc atacattaaa
    17521 ttgatgtctt ggcaggttcc acgactgacg attctggtat cgccttcaaa cgcatagggt
    17581 tcaaaaggag ctagggaaat ctctttagca tgactcaaat cacttaatgt gatgggcttg
    17641 tctaggctca ttaggatcct gtgatagcca tcaagtcggg taaaggttgt ctgtcgtagg
    17701 gctacagttg cagttgagag acgataggag aacgttcttt ctttgtagtc tcccttttca
    17761 ggataaagga acaattggtt ggtttcaccg ccagaccagt cagtttgagt gtaatctgct
    17821 ggcgttttga ccattacggt tgtcatctgt tctctccttg tttccaatta gaatagtcct
    17881 gtgatgttgc cctcctcgtc aatatcaatt gtttcgctgg caggtacttt tggaaggcca
    17941 ggcatggtca tgatagcacc tgtcagggca acgataaatc ctgctcctgc ggacacttta
    18001 aggttgctaa tagtcactat aaagtcagtt ggtgcgccaa gttttttggc atcgtctgag
    18061 aaggaatact gggttttggc catgcaaatt gggtagttgc caaagccgag gcgttctaag
    18121 tcagccagtt cacgtttggc tgcgggggtc aaggtgatgc ctttaccacc gtaaaccttg
    18181 gtaacgattt tagtcagttt ggtttcaatg ctatcgtctg catcgtaaac aaagcggaat
    18241 tgattgtctt gctcggctag tgtcacgact ttttcagcta attcacggcc accagctccg
    18301 ccatttgccc aaacatcaga aatcacaacg tcaacgccac gtttgtcgca ggcatcatag
    18361 acagcttgta attcagcatc ggtatcaagc gggaatttat tgatcgccac gacaactgga
    18421 aggccataga cgtcttggat attagccaaa tgtttgtcaa ggttaggtaa accatccaca
    18481 acggcctgaa cattttcagt agccaggtct gcttttggca cacccccatg cattttaagg
    18541 gcacgaatag tggctactag gacaacggct gctggacgaa ggccggacat gcggcatttg
    18601 atgtcaatga atttttcagc tccaaggtca gcaccaaatc cagcttcggt aacggcataa
    18661 tcaccatatt tcaaggctag tttggtggct aggacactat tacagccatg ggcgatatta
    18721 gcaaatgggc caccgtgaat gagggctggt gtgtgttcca gggtttgcac gagattagga
    18781 tgaatcgcat ccttgagcag tgcggctagg gcaccaccag ctttcaagtc tttagctgtt
    18841 acaggttcgc cttgatagtt gtaaccgatg atgatttttt caaggcgggc tttgaggtca
    18901 gaaatatttt cggataggca caggattgcc atgatttcag aggcgacggt aatgtcgtaa
    18961 ccatcctcac gagggacccc gttgaccttg ccttgcaagc cgtcaacaat atgacgaagc
    19021 tggcggtcat tcatatccac cacacgtttc caagtaatac gtctggaatc aatgcctagg
    19081 ctattgccat ggtgaatgtg gttatcaatc aaggcagcta ataagttgtt ggcgacaccg
    19141 atggcatgaa agtcccctgt aaagtgaagg ttgatgtctt ccattgggac aacttgcgcg
    19201 tggccaccgc cagcagctcc tcccttaacc ccaaaaacag gaccaagaga aggttcacga
    19261 agggcaataa ctgctttttt tccaatggca gaaagagcat ctaccaatcc aacggaagtg
    19321 gtggttttgc cttcaccagc tggtgttggt gagatagcag tgacaagaat gagtttgcca
    19381 tcaggtttgt cttttaaggc gaccagttga cgggcatcaa tttttgcctt gtacttgcca
    19441 tagagacaga gcgcttcctc gtcaatccct aattgattgg caaccttact gataggctcc
    19501 atagtgacag agttggctat ttcaatatct gataaaacca tagttactcc tttacttgtt
    19561 aaacaatatc gagaattttc gtgtagatgt catctgccaa gtgacagccc ttgtctaata
    19621 aggcttgtcc tttttggcga tagtcggtaa caaagtcttc atctttgatg ccagacagat
    19681 taatcaaaac attcaaccaa gcgctttgta aacctgcttt taagtttaaa gcagcaactc
    19741 ctaagtcgct agcagcattt gtattagact taccgacagc tgttgcagtg atctcaagtg
    19801 cttcgaccat gagggtcatc atctcaaaag gtgattgtgc agctgttttc aaggcctttt
    19861 gcatggcagt tcgtctagca gccttgtctt cgtcagtctc ttttggcata tcaaaaacgg
    19921 cagaaaccag gttgaaggct tcggtatctt tgtcaatggc tgcaagcaag ctagcttgta
    19981 gagcggtgct tttagcatgg atctctgtaa taatagcttg gtagtcagcg tattttttct
    20041 taccaagagt caattcacag accatcttgg ttaaggaaat gccattagcg ccagagagag
    20101 cagcagcaga accaccacct ggagcagggg catctgagcc aaggacttta gcgaaatctg
    20161 ttaagcttaa atcaactaaa cccatagaga acctcctaac ctagcaaatg gttttcaaga
    20221 atctgtttat ggtagtcaaa gtcctcaact tgtaagtagt attcagccac atcgattaag
    20281 gccttggctg gcgctaagcc gatgatttca gaaccaataa cattcacacc gtagcgacgc
    20341 gcttcaaatt tgatggtttc aaaagtacgg taaagagaac atttttcaaa gttaaccatg
    20401 ttcatggaaa cttgggcaat atgacggtct tctaacatga caccgatagc cttacaatat
    20461 ttgtagccac cacctgaacc gcggataatt ttagcaattt tatgagcaat gtcgatattg
    20521 tcagtatcca aattaacatt gaaggcaacc aatggcatgc gagcaccaac ggcagttacc
    20581 ccagcagttg ggtgaatctt acgatctccg tagtcaggtg cccaatcctc ttccaacagt
    20641 ttttctggca tgccttcgaa ttgtccttta cggactttgg ccaggttttg acgttctggg
    20701 cgtgtggcag agtcttcgta aaggaagatt gggattccaa gttcacggtt aatccgttct
    20761 gcgacttgtt tggaaatctc aacacattct gctgtggtga tgtctttgat tgggacgaac
    20821 gggcagacat cggtggcacc catacgaggg tgttcgccat gatgtttggt catgtcaatg
    20881 ttttcagaag cgtatttcac cagttggaaa gctgcttctt gaatagactg gtcatctcca
    20941 actaaggtaa acacgctccg gttgtggctc gcatcagaag agtagtcgag aagggtcact
    21001 ccaggaatac ttttggtagt cgctaccaag ccgtcgataa cagcctgatt ttggccttct
    21061 gagaagttgg gaatacattc aacaattttc gccatgttta atctcctttt atgtcattga
    21121 gttactcatt aagagaatag gttacctaat ttagataacg cttacatttt tagctagcag
    21181 ttattagtga aataattgat ctaccgctga agtcacaagg tcatcatcag ccaagtatgg
    21241 gatagtgatg tgatcagtac ctgcatggag acggttgtat tcaatagcgg tttcaatagc
    21301 gtgctcattg cgagcccaat tgcgacgagc aacaccaccc atggtgtccc aagcaatggc
    21361 agatttaata atctcatcaa tgcgttcgct gccatctaaa acaagaccga aaccaccatt
    21421 gatggcttta ccaataccag ttccgccacc gttatgaaga gccactaaac tcataccacg
    21481 cgcagcgtta ccagcgtaac attgaaccgc catatcacag gtcacattgg aaccgtcttt
    21541 aatgtttgag gtttcacgaa atggtgagtc ggtaccagac acgtcatggt ggtcacgacc
    21601 aatcatgaca ggaccaatct tgcctttgcg gaccaattca ttaaatttaa gggcgatagt
    21661 cacacgaccg atacagtctt ggtaaaggat ccgggcttgg gtaccaacta cgagttgatt
    21721 tttctcagca tcacgaatcc agttgtagtt atcacggtct tgatagcggc ggtcaggatc
    21781 gatagcttcc atggcagctt tgtcagtggc caccaagtct tcgtgtttac cactcaagca
    21841 gacccaacga aaaggtccat agccatagtc aaaaagcata ggtcccatga tgtcttcaac
    21901 ataagaaggc cagataaagc cgtctttgtc gttacgacca ttcttagaaa tttctgtaat
    21961 accagagtcg taaacagact tcataaaggc attaccgtag tcaaagaaat aggtgccatt
    22021 ttcagttaac gtcttaatag cttcaaaatg ccgtgccaag gtatcatcaa ccatttggtg
    22081 gaaggtatct ttatcttctg ccaagagccg ggttctttcg tcaaagctaa tacctactgg
    22141 gcagtagcca ccgtcataaa cgttgtgaca agaggtttgg tcagacaaaa gatcaacatg
    22201 aatctgcttg tcattgacgt actctaaaag atcaacgata ttaccatggt aagcaatcga
    22261 agtggactct ttagcatcta tggctttttg ggctaattgc agagcttctt cagggctctc
    22321 agcaatttgg gtaacccagc cttgcgaatg acgggtttca atccgagatt ggtcaacttc
    22381 agcaataatg gcaactgctt tagcaatttc agcagcctta ccttgtgccc cactcatgcc
    22441 tcctaaacca gatgagataa agagtttacc ggtcaaatca ccatcgtcag ccaccccaag
    22501 tttgagacga ccagcattta agagggtatt aaatgttcca tgaacgattc cttgtgggcc
    22561 aatatacatc caaccacctg cggtcatttg accatagttg gtgacaccca tctcttcagc
    22621 gatttcccag tctttcatgt tgtcgtattc acccaccaaa aggccattag taataatcac
    22681 acgaggcgct tcaggtttgg atttgaaaag accaactgga tgaccagact caaccaccaa
    22741 ggtttgttca tcagtcatga cttctaggta ttttttaatc aaacaatatt gcatccagtt
    22801 ggcacaaaca gaaccagttt ctccataagt gactagttcg taagggtaca aggcaatctc
    22861 aaaactcaag ttattgtcaa tcatgacctg catggccttg gcagctgtac aatttccctt
    22921 gtactcgtta atgggtttgc cgtaaatgcg gtcttttgga cgccatctgt aaccatagat
    22981 gcgtcccctt gttttgagtt cttccaaaaa ttctggaatg acttcttcgt ggaacttcgt
    23041 aggtacatag cgtagggcat ttttcaaggc gatttcggtc tgagcctggg tcaatcgaaa
    23101 acctctgtct ggtgctcttc ggataccttc tttgaagaca gtcttttccg gtaaaacatc
    23161 gtctaactta acagtcatag cggcagctat gtcagtttca ctgtaaaatg acatagaaaa
    23221 ctcctcctca actccttttt attgtcgaga ttagtctaac ttagtctagt ctataaaaag
    23281 tcaaccacgc tttgaaatgc gaaaaaagtc taaaaaaaaa tcaaaacctc attttttgac
    23341 taaaatttga cgcttaatca gtacacttga ggcatgacag ataaagtcag tcagaggtta
    23401 attgttgtta ccaaagttgc aaagatcgca actattgagt gggtgagggg ggactagtaa
    23461 tggctattaa tcagttgaca aacgatgtta aaaaaggagg tttttatggt tgctgatgtc
    23521 ttattaacac attttaatca actattttgt ctaaatgatc caggtcatcc tttaactggt
    23581 caagaaatga aaaaagccac tatcgttgaa gatggctata tagccatcaa agacggcctc
    23641 attgtggctc ttggttctgg tgaaccagat gctgagcttg tcggtccaca gactattatg
    23701 cgttcttata aaggtaaaat tgcaacgcct ggtattattg actgtcatac ccatttggtc
    23761 tatggtggca gtcgtgaaca tgaatttgcc aagaaattag caggggtgtc ttacttggat
    23821 attttagctc aaggtggtgg tattttaagc acggtgaggg ccactcggtc agccagcttt
    23881 gacaaccttt atcaaaaatc caagagattg ctagactaca tgttgcttca tggagtgact
    23941 actgtcgaag cgaaaagtgg ctacggcctt gattgggaaa cagaaaaacg ccaactggat
    24001 gttgttgctg ctttagaaaa agaccatcct attgatttgg tgtctacgtt tatggcagcc
    24061 catgcgattc ctgaagaata caaagggaat cctaaggctt atctcgatgt gattatcaag
    24121 gatatgcttc ctgttgttaa agaggaaaat ctggcagaat tttgtgatat tttctgtgaa
    24181 aaaaatgttt ttacagctga tgagtcccgc tatctgttaa gcaaagccaa agaaatgggc
    24241 ttcaagcttc gtattcatgc agatgaaatt gcgtctatcg gcggagttga tgtcgcagca
    24301 gagctcagcg cagttagtgc agagcatttg atgatgataa cagatgatgg tatcgctaag
    24361 ttgattggtg ctggtgttat tggtaatttg cttccagcca ccacctttag tttaatggaa
    24421 gacacttatg cccctgctcg caaaatgatc gatgccggca tggctatcac tctctcaaca
    24481 gatagtaatc caggcagttg cccgactgct aacatgcagt ttgtgatgca attaggttgt
    24541 tttatgttac gtttaacacc gattgaagtc ttaaatgctg ttaccattaa tgcggcctac
    24601 tctgtcaatc gtcaggagag ggttggtagt ttgacagttg gtaaagaagc agatattgcc
    24661 atctttgatg ccccaaatat tgattaccca ttttatttct ttgccaccaa cttgattcac
    24721 caagtctata aaaaaggaca actcacggtt gatcgaggtc gtattcttta aaaaatttca
    24781 aaaagaagct ttgagcgtta gctcaaaact tcttttaaat atgctgtttt attgacgaat
    24841 caagtagtca aaggcaccga tggcagcagt agcaccagac cccatggaaa taatgatttg
    24901 tttataagct gaatcagtac agtccccagc agcaaagatg ccgggaatat tggttgaacc
    24961 atgtttatcc acaatgattt ctccgcggtc ggttaggttg acaccactat ctttgagcca
    25021 agctgtgttt ggaataagac caatttgaac aaagacccct tcaaggtcaa ggtgtttgtc
    25081 ttcaccgcta tcacgttcag tgtagttgag accagtaaca tgatcgtcac caacaatgtc
    25141 tttagtagca acatttttga taatagtcat gttagcagta tcagctgcgc ggtcttgaag
    25201 cactttgtca gctttgagct caggcaagaa ttctaacacg taaacgtgtt tagcaagacc
    25261 agccaagtca agggccgctt ctaaaccgga gttaccacca ccgataacag caacatcttt
    25321 gccttcaaag agtgggccat cacaatgagg acagtaagtc acccctttat tacggaattc
    25381 atcttcacca ggaacattaa tgttacgcca tttggcccca agagctaaga tagcagtttt
    25441 ggcttgtaga accgcaccat tagctagagt aacctcgatg ttttcttttt tctcaataga
    25501 agtagctagt tgtgctttga taatatcaac atcataagac ttagtatggg cctccacttc
    25561 ggccattaat tttggtcctt cagtgtaaag ggtaccaatc atgttttcga tgccgaccgt
    25621 ttccatgacc tgaccaccaa aggtttcagc aagtaaacct gtttttagtc ctttacgagc
    25681 tgcatagata gcagcgctat tgcccgcagg gccaccacca ataacaagga catcatagag
    25741 gcctttatca gcaaaggctt cctcagaaag tgggccagcg atttgttcta agagttgctc
    25801 aatggtagcg cgaccagaag tgaattcttc cccgtctaaa aagacagtag ggacagacat
    25861 aatgcctttt gctttcactt catcttggaa catgccgcct tctaccatag tgtgtgaaat
    25921 cttgtcgttc aagacggaca taatgtttaa agcttgaaca acatctgggc agttatggca
    25981 ggttaagctg acataagttt caaaatgcaa cggacggtca atggccttga tgcgatcaat
    26041 aacatcttga tccacttttg gagcacgtcc agatacttgc aagagagcaa gaataaagga
    26101 cgtcaattcg tggcctaaag gaagaccagc aaagacaacc ccactaccgt gacctttttt
    26161 cgcaacctta aagcttggtt gtcgatctaa agtgatgttt tcaatagaaa tacgctcaga
    26221 catagcagct atttcttcca caaagtcttt gactttttga gactgctcat tgtcaccaag
    26281 ggaaacttgc aaaacaagat cagcttctaa cagggtgagg tattgggcaa gttgttcttt
    26341 aatatcagga cttaatgcca tagctttcct cctaaaattt attaaatttt accaactaaa
    26401 tcaagacttg gtgtcaaagt ttcagcgcct tctttccatt tagctggaca aacttcacct
    26461 ggatgtttac ggacgtattg ggcagcgtga attttatcaa tcaaggtgct agcatcacgt
    26521 ccaataccat cagcattaat ttccatcatt tggataatac catctgggtc aacgataaat
    26581 gttccacgtt gagcaagtcc gtcttcgcca agcacttcaa aggcttgtga aataaggtgt
    26641 gaagggtcac caatcatagg gtatgtgata gtgccaacca catctgaatc atcatgccaa
    26701 gctttatgaa caaaatgagt atcagtagag acagaataaa cttctacacc aagagatttc
    26761 agtgtttcgt attgctcttg aaggtcaccg agttcagttg ggcaaacaaa tgaaaagtct
    26821 gctgggtaga aacaaaaaac tgcccatttt cctttaacgt cttcatttgt aacagtgatg
    26881 aattttccat cgtgataagc ttgagctgaa aattcagcaa tttcttttcc aattagagac
    26941 atatagttgt cctccttttt tatagtttta tacttgtatt ataaa
//
LOCUS       DAUGFP010000020        26659 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.020, whole genome shotgun sequence.
ACCESSION   DAUGFP010000020 DAUGFP010000000
VERSION     DAUGFP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26659
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.020"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..215)
                     /locus_tag="VH663_000884"
     CDS             complement(<1..215)
                     /locus_tag="VH663_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEQ8174099.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIG"
     gene            complement(629..934)
                     /locus_tag="VH663_000885"
     CDS             complement(629..934)
                     /locus_tag="VH663_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEQ8174100.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            complement(946..1365)
                     /gene="ruvX"
                     /locus_tag="VH663_000886"
     CDS             complement(946..1365)
                     /gene="ruvX"
                     /locus_tag="VH663_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEQ8174101.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            complement(1362..1631)
                     /locus_tag="VH663_000887"
     CDS             complement(1362..1631)
                     /locus_tag="VH663_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEQ8174102.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            complement(1745..2143)
                     /gene="spx"
                     /locus_tag="VH663_000888"
     CDS             complement(1745..2143)
                     /gene="spx"
                     /locus_tag="VH663_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEQ8174103.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
     gene            complement(2434..3570)
                     /gene="recA"
                     /locus_tag="VH663_000889"
     CDS             complement(2434..3570)
                     /gene="recA"
                     /locus_tag="VH663_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEQ8174104.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPQLFD
                     EIDRKVRVKFGLLEESEEESAMAVASEETDDLALDLDNGIEIED"
     gene            complement(3659..4930)
                     /locus_tag="VH663_000890"
     CDS             complement(3659..4930)
                     /locus_tag="VH663_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEQ8174105.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSNLVILCGGLGPTKDDLTKQTLAKYLRKDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPM
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            complement(4999..5559)
                     /locus_tag="VH663_000891"
     CDS             complement(4999..5559)
                     /locus_tag="VH663_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEQ8174106.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLHDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVAAFTSEEMADALENLSIIRHKLKLAATVNNAIA
                     VQKIQEEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            complement(5569..6165)
                     /gene="ruvA"
                     /locus_tag="VH663_000892"
     CDS             complement(5569..6165)
                     /gene="ruvA"
                     /locus_tag="VH663_000892"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEQ8174107.1"
                     /translation="MYDYIKGQLTKITAKYIVVEANGLGYMINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLDGKFVEAPQETGNTKARSNKAGNTQLDEAIEAL
                     LALGYKATELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            complement(6167..7387)
                     /locus_tag="VH663_000893"
     CDS             complement(6167..7387)
                     /locus_tag="VH663_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8174108.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFAGILLVEIASSFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFDVAYYYFDETRPETHAFDHGKGLLASFQNYRQVFQDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQFYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            complement(7398..9380)
                     /gene="mutL"
                     /locus_tag="VH663_000894"
     CDS             complement(7398..9380)
                     /gene="mutL"
                     /locus_tag="VH663_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEQ8174109.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLVISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQSRGLYYDPQKNDFFVKESAVSEKIPETDFYSGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNTFTETDHPNLDLKNRQKLSQMLNRLE
                     NEEQSVFPGLDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGDVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            complement(9509..12064)
                     /gene="mutS"
                     /locus_tag="VH663_000895"
     CDS             complement(9509..12064)
                     /gene="mutS"
                     /locus_tag="VH663_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEQ8174110.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFNSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRTGFDERLDHYRKVMREGTGWIADIEAKERQASGINNL
                     KIDYNKKDGYYFHVTTSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKTLATVDVLQSLAVIAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIISVPS
                     QVESSSAVRQGQLSLFGDEEKTHEIRQALEAIDVMNMTPLQAMTTLYELKKLL"
     gene            complement(12051..12403)
                     /locus_tag="VH663_000896"
                     /pseudo
     CDS             complement(12051..12403)
                     /locus_tag="VH663_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
     gene            complement(12400..12837)
                     /gene="argR"
                     /locus_tag="VH663_000897"
     CDS             complement(12400..12837)
                     /gene="argR"
                     /locus_tag="VH663_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ8174111.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVSTPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            13128..14819
                     /locus_tag="VH663_000898"
     CDS             13128..14819
                     /locus_tag="VH663_000898"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEQ8174112.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKAKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFAKMGYQPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAEIDHTVDEEARGWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLDLLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHAVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
     gene            14907..15215
                     /locus_tag="VH663_000899"
     CDS             14907..15215
                     /locus_tag="VH663_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ8174113.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKACHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(15242..16114)
                     /locus_tag="VH663_000900"
     CDS             complement(15242..16114)
                     /locus_tag="VH663_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ8174114.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(16157..17035)
                     /locus_tag="VH663_000901"
     CDS             complement(16157..17035)
                     /locus_tag="VH663_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ8174115.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVMGILSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGLVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     DELGRGVTYLTGEGAYSGRRKKVIYVALGRRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            complement(17055..17996)
                     /locus_tag="VH663_000902"
     CDS             complement(17055..17996)
                     /locus_tag="VH663_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ8174116.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIIPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            complement(17989..19737)
                     /gene="aspS"
                     /locus_tag="VH663_000903"
     CDS             complement(17989..19737)
                     /gene="aspS"
                     /locus_tag="VH663_000903"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEQ8174117.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVKGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTNGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANAQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            complement(20075..21355)
                     /locus_tag="VH663_000904"
     CDS             complement(20075..21355)
                     /locus_tag="VH663_000904"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEQ8174118.1"
                     /translation="MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKDMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDIYLA
                     VLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFENISEQLLS"
     gene            21575..21757
                     /gene="rpmF"
                     /locus_tag="VH663_000905"
     CDS             21575..21757
                     /gene="rpmF"
                     /locus_tag="VH663_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEQ8174119.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            21773..21922
                     /gene="rpmG"
                     /locus_tag="VH663_000906"
     CDS             21773..21922
                     /gene="rpmG"
                     /locus_tag="VH663_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ8174120.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            22216..22830
                     /locus_tag="VH663_000907"
     CDS             22216..22830
                     /locus_tag="VH663_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEQ8174121.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGEILEKYSRWFIAVVYLGLGIYILIENNSFDMLWAVLG"
     gene            22842..23180
                     /gene="cadX"
                     /locus_tag="VH663_000908"
     CDS             22842..23180
                     /gene="cadX"
                     /locus_tag="VH663_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEQ8174122.1"
                     /translation="MKKDSICQVNVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLTVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            23374..24147
                     /locus_tag="VH663_000909"
     CDS             23374..24147
                     /locus_tag="VH663_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174123.1"
                     /translation="MLYLNTQTDSDYKEIIFGDIAKFVENMFYHYFSSILFRDLETVD
                     KRMYSFSDDDLISIQSSCLRLSKTFANFNIQRKNFLASDETEMDTFHKKDDIDITVGE
                     KSYNLARSFRTSTINELTVFNFEEMFDIIWLMLGDNLIKSFEVNVCGILFELDGNGIP
                     STFRQENIDPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFLVDDILERMYKENIPK
                     SYLTSVPLVISKKARSTLSIKSNGSQKIDKSKFAQILSL"
     gene            24213..24965
                     /locus_tag="VH663_000910"
     CDS             24213..24965
                     /locus_tag="VH663_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ8174124.1"
                     /translation="MAIPDLDKFSSSLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            24962..25565
                     /locus_tag="VH663_000911"
                     /pseudo
     CDS             24962..25565
                     /locus_tag="VH663_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26041..26322)
                     /locus_tag="VH663_000912"
     CDS             complement(26041..26322)
                     /locus_tag="VH663_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ8174125.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGI
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRSALRIERFKKTYDVQTYR"
     gene            complement(26329..26373)
                     /locus_tag="VH663_000913"
                     /pseudo
     CDS             complement(26329..26373)
                     /locus_tag="VH663_000913"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8364 a         5644 c         4486 g         8165 t
ORIGIN      
        1 cctatatata agttttggga ctgaagacca ttggctgcat ctgttaaaaa aggtactgca
       61 attgttaaag aactgataat aatgctaaag aaaagcagat agcatttatg aagccgaggt
      121 actttagttt catattgagt ggtgtgtact cgtgggccat cgttggccag ttctatcgtc
      181 tcttttcgga tgaattcatc gtttaactcg atcactattt ttctccttga aatatcatat
      241 tctctaacga ttcaataaaa gcaatatttc ttcatgtcac tagtctaaca tagctattat
      301 ccattatttt tttagtatga gataaataac atgactttgt attacacata gcaaagtgac
      361 agtgcataaa caaagtaagt tgaaaaatga tattatcgtc aaagaatatt ataaaacaag
      421 tataacagaa tcttttattt agtcaatttc tgatatattt tcacatactg taataattct
      481 ctaaatctat gcaaaggttt agccttataa ttattaataa atttatcatt taattctttt
      541 atcattatca cctcctgctt atttattcga aaataacatt aaaaaaatag ccctcacggc
      601 tactttttaa catagtgtca ctaacaattt agttctcatc tagaaaacta ttaaatacct
      661 cttcaatcat atcccattca gcatctgaat cctcaggaat cggttgtaaa tccccttcag
      721 taccatcttc attttcagta aatgaatagg cttggatttc aatttcacca gactcatctt
      781 cttcagatcc cgctggaacc aagagaacat aatttttacc aaactcttca cgaccatcaa
      841 ttgttaataa aatttcaaac aaggtttcat ttccttgttc atccacaagt gtgataactt
      901 cgtgttggtg gtcgttttca tgattatgtg tcatttttta tctccttaaa aattccgatt
      961 taaatagttc tgtaaaatga gttgtgcagc taatttatca atgacttttt ttcgcttacc
     1021 acgactaatg tcagcttgct caataagcat acgctctgcc tcaacagtcg tcaatcgttc
     1081 atcttgataa tggactggaa gtccaaaaag gtgctctatg tggtttccat aagttatact
     1141 tgcatcaaca cgtggaccat tggtgttatt catatttttt ggcaatccaa taacaaactg
     1201 ttcaacttgg tactgcttaa caagctcttc taaacgtgta aagccaaatt ctgctttttc
     1261 ctcatcaatc ttgataattt caagtccttg ggctgtaaag ccaagaggat cactaatagc
     1321 tacaccaacc gttttcgagc caacatcaag tcccattatt ctcatttaac atcaattcca
     1381 tttccttgta aatagtagcg aacaagttct tctacaattt catcacgttc atatttacga
     1441 atctgatttc gagcatcatt ataacgagga acataagctg ggtccccact caagacataa
     1501 ccaacaattt gattaattgg attataaccc ttttcatcaa gtgaatgata gactgctgtt
     1561 aatgtttcac taatttgtcg tttgtcgcca tcatccaact taaagcggac tgtttcatct
     1621 gtaaatccca tacttacacc ttctttctaa aattaagtgc tatgctttat tatagcttat
     1681 ttctattaaa taaacaagaa tttcaagcga agaaggtatt taaaaaaaag ctctacaaga
     1741 gcttttagag tgcagcacgt aatcgtgctt cagtattttc aatattacga atagagcgag
     1801 gtaaaaaagc acgaatatcg tcttctttgt agcctacttg cagacgttta tcatcaatca
     1861 aaatgggact tttaaggatg cgcgggttat cttgaatcaa atcaataacc tcactaacac
     1921 ttaattcttc aatatcgcaa tcgagagctt tggcataacg atttttagat gaaacaatgc
     1981 tctccactcc attttcggtt tttgataaaa ttgctaaaat ttcttcttta gttagcggtt
     2041 cttttcctaa gttctgttct ttataagcaa gcttatgggc atttagccaa gttttggctt
     2101 tcttgcagct cgtacaactt gaaatcgtgt aaattttaat catgttgctg actcactttc
     2161 tatttcgtga tagctttatt gtagcacagt ttcttatcta taaatatagg tctttggacc
     2221 tattttttta attttaaaga tattatctta gtaaaataag gtttttcacc tgtattccct
     2281 tgaattatta gttattttaa ttttatatca gttaacctaa gttctgctct ctcacatctt
     2341 tttaaatgat ggtttctatt cacccacaca cctatgaaca gtagttctac taacctttaa
     2401 aaatacattt taaaaagccc tgaagggctc tttttaatct tcaatttcaa taccattatc
     2461 taaatctaaa gcaagatcat cggtttcttc tgatgctact gccatagcag attcttcttc
     2521 gctttcttca agcaaaccaa atttaacacg tactttacgg tcgatttcat caaacaattg
     2581 tggatgatcg gccaaataac gcttagcatt ttcagaacct tggccaatct tctcaccatt
     2641 ataagagaac caagcacctg ctttttggat aatgtccaaa tcagaagcaa ttttcacaag
     2701 ctcccctgta cgagaaatac cttccccata catgatttca acttctgcta ccttaaatgg
     2761 cggagcgacc ttgtttttaa caaccttgat tttggtctcc ttaccaatgc tgctatcttt
     2821 ttggtcacca gttcctttaa tttgagttgt tccacgcaca tccagccgaa cagaagcata
     2881 gaatttcaaa gctcgaccac ctggtgttgt ttcaggattt ccaaacatca caccaacttt
     2941 ttcacgcaat tggttaataa agatagcgat agtttttgtt ttattaatag aagctgataa
     3001 tttacgcatg gcctgactca tcatacgtgc ttgcaatccg acatggctat cgccaatatc
     3061 accatcaatt tcagcacgtg gcactaaagc tgctactgaa tcgacaacaa ccagatcaac
     3121 cgcaccagaa tcaatcaatt tacctgcaat ttcaagtcct tgttctccag aatctggttg
     3181 agacaagagg agttcatcaa tattaacccc aagcgcagca gcataagctg gatcaagcgc
     3241 atgctcggca tcgataaagg ctgcgattcc accttctttt tgagcttgtg ctacagcatg
     3301 taaagccaca gtcgttttac cggaagactc tggaccatag atttcgatga tacgtccttt
     3361 aggataacca ccagctccaa gcgcaatatc aagagctaga cttcctgaac tcataacctg
     3421 aactttttgc tctgcacgtt cccctaatcg catcactgcg cccttaccaa aatctttttc
     3481 aatatttttc aaagcatcat caagggcttt gcgacgttcg tcaccaaact tcttagtgat
     3541 ttcttcattc ttttttaatt tttttgccaa tactttctcc tcttttttaa caaaccatcg
     3601 tcagcaggat taatctacat aatcgtaaac catatcctac tatagctact aaataacatt
     3661 atagcaaatt ctcaagtttt aataaagttt tacggaccat attaaaggca tgtaaagtag
     3721 cgatatagcg cacatccaaa cgacttcgcc cgctaatcaa aacctttatt gattctacct
     3781 tattttgagt ggcaagacca atgaaaactg tacctgcagg ctgttcctcc aacatatctg
     3841 gtccggcaac acctgttaaa cttaccccaa tatcagcacc agtcaataac cttgcttgct
     3901 ccgccatccc ctcggccgta taagcactaa caacgccatg cgatttcaac tcctctaaag
     3961 gaaggcctag cattttcgct ttttcttcca tgctataagt cacaaagcct ccattgaata
     4021 cttgagaagc tcctggaaaa ctcgccaaag ttgactgaaa taatcccgcg gttagacttt
     4081 ctgctgctgt aattgtctta tcatatttta ctaagagctc aaatgtctca cgcgctaagg
     4141 aattatcctc cccatagcca taaaagacat cagctaaggg ttgaccttca agagtttttc
     4201 gggatagtag ctgcgcttct agctgaccta actttttatc tgccagagct tggttttcag
     4261 tttttgttga taagcgaaga gtcacttcgc cagtcttagc atacggagca atggttggat
     4321 cagtttgatt ctcaataaaa tctgacaaga ctgttaccaa ctgactttcc ccaataccaa
     4381 aaaagcgtag tacctttgaa tacaatgtac tgtattgttt tgacagaagt ggtaccaatt
     4441 cttcatttac catcggcttc aattcacttg gaggacccgg taagacaaca taactaatac
     4501 catcgactgt gatgaaccca ccaaccgcaa gaccagtttt atttggcaaa gggattgacc
     4561 cttcaatcac ttgtgcctgt cgctcattat ttggtgtccg tgatgaaggc ttgcgcttag
     4621 caaaaaagtc atctagtttc tgacaagctt gctcatcata aaccaagtct ttcctaaggt
     4681 actttgctaa agtttgtttg gttaaatcat ctttcgttgg accaaggcca ccacataaaa
     4741 ttaccaagtt actacgctga ctagcagttg taatcactga aagtaaacgc tcctcgttgt
     4801 ccccaacagc cgtttgaaaa tagacatcaa tacctagctc tgccattttt tccgacagaa
     4861 attgagcatt ggtattcaca atttgaccag tcaaaatttc ggtacctact gcaatcagtt
     4921 cagctttcat ttctccacct acttatctat tcgtaatctg atctcatttt atcaaaaaat
     4981 cttccaattc acgaagaatt agaagattct attgaaaaca caagcctctt catggtcatt
     5041 taccaaaccc gatgcttgca taaaagaata aaccgttgta ggtcctaaaa atttaaatcc
     5101 tcttttcttt aaatccttag ctaagcgaat agataattct gtttgagcag gaacaagatt
     5161 ctcttgattg acaaggttgt taattggctt tcccccaaca aaattccaga gataagttga
     5221 aaaactacca aactcttctt gaattttctg tactgcgata gcattgttga cagttgctgc
     5281 tagctttagc ttgtggcgga taatggatag attctctaaa gcgtcagcca tttcctccga
     5341 ggtaaaggct gcaaccgatg caatgtcgta atgatgaaaa acagttctga aagcttggcg
     5401 ttttttcaaa acagtcagcc aggataagcc tgactggtaa ctttcaaggc agagtaattc
     5461 aaaaaaatct ctatcatcat gtaggggttg accccattct aaatcgtggt aatcacagta
     5521 aagctggtta tccttaggaa cccatgagca acgtttcatg tgaaacacct agcctttcat
     5581 taatagttta agggctgatt tgatgtattg ttcggctgtc tcagaggttc cttcaaagaa
     5641 ggcacgaatt ttcttcaatt ccgttgcctt ataaccgagg gcaaggaggg cttcaatcgc
     5701 ttcgtccagt tgagtattgc ctgctttatt gctgcgcgct ttggtattac cagtctcttg
     5761 tggagcctcc acaaatttgc catctaagtc aagaaccatt tgctgcgctg tttttttacc
     5821 aattttagga aatttcatta ggtacttaat gtcactgtta tcaatggcat tcacaagtcc
     5881 ctcattatca tcaactgcca caatagcaag agctgttgtc ggaccaatac ctgatacaga
     5941 aattaatttc agaaaaacat ctttttcatc ttccgtgtga aaaccaaaca acaggtgagc
     6001 atcctcacga atcacttgat gcagataaat ggttaccaat tggttgacac tatctgtaaa
     6061 gctataagga ttggcaacat taatcatgta gcccaatcca ttagcttcaa cgacaatgta
     6121 ttttgccgta attttggtca attgaccttt aatataatcg tacatattac acctttttca
     6181 tttttgcaga aacaaccgta aaataaatcc ctagcaaagc aatgactaca aataagcatt
     6241 gcacgcctaa aggacctgta aaatggctga cagatactag gagactagcc aaaatagcac
     6301 ctaggggttg tgccattgac acaaaacctg tataagatcc tatttgggaa tgatccatca
     6361 tatcagcacg taggacttga ctagcaggaa catttatcat ttccccaaaa gttaacaaaa
     6421 caactgataa ccagatagca tagaactgcg taaaggtaaa tgccaagagc atccctagag
     6481 taaatagtaa agaaccaacc acaagctgag gtaatagttt ccatttttcc gttaatttat
     6541 ttactactgt catgaaaagg acaatcagca atgtattagt taaaaccatt aatgataaca
     6601 ttttagaact agttacttgg aaacctaaca cagccgttgg ctgaaaatac agtttcaaat
     6661 ggactggcac atagttatcc atctgcatcc agatactacc agaaaagatg gcacctaagg
     6721 taaacaagac aaaggcacga tcctgaaaca cctgacggta gttctgaaaa ctcgccagta
     6781 atcctttacc atgatcaaaa gcgtgtgttt ctggtctagt ctcatcaaaa tagtagtaag
     6841 caacgtcaaa acaaagtaca ttaacgagta ataaggctac taacaaggct aaaaaatgat
     6901 ggtcataaaa aagcccagat agccctgcac caaacatgac ggcaatattg ataaaccaat
     6961 aattgatggt gtaaacaaat cgacgattac tctcatcagt caaatcaatt aacatagctt
     7021 cataggcagg accataaaaa ctagaagcaa tctctaccaa taaaataccc gcaaaggtta
     7081 accaaggaat agctgcatta ggcaaattag ctaaaatagt cagaaaccag cctagtgttg
     7141 ttcctactga cccaatcata atgacttttt tacgaccaag agcatctgac agatgcccac
     7201 cgtataaagt tccaacaaat cccatcaaac tggtaatcat cattaagagg cctgtccaaa
     7261 acgtaccaaa gtaagtcgta taatacatgg ccataaaggg aaagatacta ctgcctaagg
     7321 taatggtcac aaagcgtacc agttgcctca gctgaatctg cttaggaagg tttaaaaact
     7381 cttgcatctc atcctctcta atattttcct agctctcgca ggctagtgtg attttcttga
     7441 attcgacgga acattttttc catatctgcc ttactaaaat taatcaatac gggtctacca
     7501 tgagggcagt tataaggatt ttgacattgg gccaattgca gtagcagatt tcttgctgaa
     7561 taatcatcca agctatgatt tgccttgata gatcgtttac aactcatcat aatagctaac
     7621 tctgctcggt aagttttgat agatacctca ttggttagaa gcaacatgtc acacatttca
     7681 tagacaccag atgcaatctc ttcttctttc atccagatag gatgctccct caaaatgaaa
     7741 gtattgtgcc cataaacttc taagaagata ccgacttcat ttaagagtgc cattttctcc
     7801 tgtaagttaa tgaaatcaga accagaaaac tcaaacaaat agggcaccaa caattgctgt
     7861 aaactactgt caacgtcgcc aatcttatca cgataatatt cgtatttaac ccgctcctgg
     7921 gcagcgtgtt ggtcaattat aaataaacca tcttttccct gagcaaagag ataagttccg
     7981 tgcatctgac caaaataatc caatccggga aacactgatt gctcttcatt ttccaagcga
     8041 ttcaacattt gagataactt ttgtctgttt tttaagtcaa gattaggatg atcagtctcg
     8101 gtaaaggtat tctggggacg acttgcgtgt ttaaccgaag aaggtcctat aacttcttct
     8161 gagtggggta acagctctgc tttttcaacc tttacactgt tgtcaacagc gccggaataa
     8221 aaatcagttt caggtatttt ttccgagaca gccgactctt tgacaaaaaa gtcattcttt
     8281 tgaggatcat aataaagccc cctagactgt aaaggaagtt gtgtttgctc tggtttagag
     8341 aaatgtcgtg tgcttgattt ggctaagttt tccagagcat ctggaatcaa atcttgttcc
     8401 ttaagactct ctgaaatagc cgtgctaatt aaagccatta attcacgctc ttttgaaata
     8461 cgaacctctt gttttgtggg atgaacattg acatcggcca aatagggatc aatctgaata
     8521 tcaatcacaa caattgggaa acgtccaacc atgagcttag aaccgtaacc atcaagaatt
     8581 gctcgattaa gcaagaaatt cttgatgtaa cgcccattaa ccaaaatcgt catgtaatta
     8641 cggttggcgc gtgtcaactc tggcaagcta acatagccgg aaacttcaaa atccaaatca
     8701 gcattagaga taaccagcat ttttttggta gtatttaaac cataaatccc tgcaattgct
     8761 tggcgtaaat cgccagttcc tgatgtctga gttaattggc gaccatcact aatcagtgtg
     8821 aaagcaactt ctggatgtgc caaactcaac cggttgacca catctacaat gtgagctaat
     8881 tctgcctgta aacttttcat atatttgaga cgagcaggcg tgttgtaaaa aaggttttca
     8941 accttaattt tggttccagt aggagttgag atcgcttcaa gtgtctcaat ttccccacca
     9001 gtagctataa gaagagaacc gtgagtgact tcttttgttg ctgtttttat cgtgatttta
     9061 ctgatagagg caacagacgg taaagcctct ccacggaagc caagtgttct aattctaaac
     9121 aaatcactct gactcttaat tttactagta gcgtggcgac gcagacttaa aggtaaatct
     9181 tcatgagaca ttccctcacc gttgtctgta acctgtatca tcttaaggcc agactcttca
     9241 atttcaacgg taatctggct acttttagca tcaatagcat tctcaaccaa ttccttgaca
     9301 acactcgctg gcctttctac aacttcacca gctgcaattt ggttggcgag aacttccggt
     9361 aattcaataa tgtttgtcat aatttcctcg cattcttatg gactattata acacacttaa
     9421 taaatagccg aaggcctcgg accgtttttg acagaacaaa aagatgcttt ctatattgaa
     9481 ggcatctttt tcattttcag ctgaaagact ataacaactt tttcaattcg taaagggttg
     9541 tcattgcttg aagcggggtc atgttcatga catcaatagc ttccagtgct tgcctaatct
     9601 catgagtttt ctcttcatca ccaaaaaggg ataattgccc ctgtctaaca gcgctgcttg
     9661 actcaacttg tgaagggact gatattatct cagtagatcg tgactgtgtt tctaaacggg
     9721 tcagaacttc gtctgctctc tttaataggg attttggcag tcctgctatt tttgccacat
     9781 gaataccgta agatttatcc gccggtccct cagcaatctt atgaaggaag gtaacatcgc
     9841 catctttttc aagcgttgct acatgaacat tgactagact tgtcaactta gttgacaagt
     9901 ctgtcaattc atgataatgc gttgcaaata tggtcttagc accaactcta tcatggatat
     9961 attcaataat tgcctgggct aaagccatac catcataagt tgccgtacct cgtcccagtt
    10021 catcaaatag aataagagag ttgtcacttg cgcgtttgat tgcttggttt gcttccatca
    10081 tctccaccat aaaggttgat tgcccagaaa tcaaatcatc agcagcccca atacgcgtaa
    10141 aaatcgcatc aaataaaggt aaatcaacat ggtcagcagc cacaaatgaa cccatctggg
    10201 ccatgataac cgttaaggcc agctgtctca tataagtcga cttaccactc atatttggac
    10261 ctgtaatcag ctgaatactg gtctgttggt caaaagagat actattggga atgtattcct
    10321 gcactcccat aaccttttca acaaccgcgt gacgaccttc ttgaattgta atcacatgat
    10381 tatcattgaa ctgcggccgg atataatgat tggtttcagc aatgactgct aaactttgca
    10441 aaacatccac cgttgccaaa gttttagcca gtttctgtaa acgattaata taggtttcaa
    10501 cttgagctcg aatacacata aaaatatcgt attctaaact agatgactct tccctagcct
    10561 ctaacatctg accttcaatc ttagccaatt ctgctgttcc ataacgttca gagtttttta
    10621 aagttgcctt tctgaaaaaa tgctcaggca ctaagctaag atttgaagtc gtaacgtgga
    10681 aataatatcc atctttttta ttgtaatcaa tttttagatt attaatgcca cttgcttgac
    10741 gctctttagc ctcaatatcc gcaatccagc ctgttccttc tcgcatcact ttacgataat
    10801 ggtccaagcg ctcatcaaaa ccagtacgaa taatactgcc ttcactaata gttgctggtg
    10861 cgtctggatc aatggctgtt ctaatcaagt attccaactc aggcaaactg tcaatatcat
    10921 tgacaagttt gtcaacacaa ggactgttaa aagactctaa gatagctttg atataaggca
    10981 cttgggctaa ggtatgcccc aattgaagta aatctttcgg atttgccttg ccaaaagaca
    11041 cgcgactaga taagcgttcg atgtcgtaaa caccttttaa actattgctt aaatcggttc
    11101 gctcaataaa agcattcaga aaaacttgaa taatttcttg acgctctaaa atagcttctt
    11161 tagaaaccaa aggtcgatca atccatgagc gcaaaagcct catccccata gctgtcttgg
    11221 tttcatctaa aagccaatac agacttccat gttttttatt agtcctagca ttttctacca
    11281 aatctaaact tgacttagtg gcatacgaca tctgcaaata atccttgatc tcatagtgaa
    11341 ccaaggcttg caagtggctg agttctcgca tttgtgtctt gtgaacgtat tgcaagagtt
    11401 ttcctgccgc tgtgagttct accgttgtca attggccgtc aattaaagat ttatcttcat
    11461 agaccgtttc ttcataagaa agcagcaaat tcatctgctt gaccaaaatc gtctgttctt
    11521 cttcagataa atcaaaacct agtaagactt cttttgcctt gaggttttgg atttcgctac
    11581 gaacactcgt aaagtccgcc aaatctgtca cgcaaaattc acctgtggat acatccatat
    11641 aagccaatcc ataacggcaa ccatcaaagt caacagctac caaaaagtta ttggcgctat
    11701 ctggcttagc tgaatccaca accgttccag gagttatgac ttgaacgacc tcacgcttca
    11761 ccaccccaac agcttgcttt gggtcttcca tttgttctgc gacagcaacc ttgtaaccca
    11821 actcaattaa cacatcaatg tattgttggg cagaatgatg tggcacgcct gccatgggaa
    11881 ttggattttc cgcattcttg ttgcgactgg tcaaaccaat ttctaagagt tgtgctgctt
    11941 tgacagcgtc ctcgtaaaat aattcataaa agtcacccat cctaaaaagc aaaaaagcat
    12001 ctggataatc ttttttgatg tccagatact gttgcattcc aggagaaatg ttagtttttg
    12061 ccatttgtta actccttgtt taactgatct tctatacgtt tggtgatata ctgagtcaca
    12121 tcacttgcat cttgcatctt ttctcgataa tcatcaatta atggctgatg actgagccta
    12181 tcttctagtt cttttgccct gcttgcagat actgtcgcag ccttttcttt atctactttt
    12241 tttgaaaagc agggcatctt gttgatagcg tttcatctga taagcttact ttttaaaatc
    12301 acggagctta gacagctatt gcttcacttc tttataagca acgatactag aattcatgga
    12361 tagcaagaat caattgtgtc aaagactctt aatagctagt cataggcctg cagccaaatc
    12421 tttttcaaag cgtttagcaa tctcttcgtc tctgcaaatc acaagcaagg tatccgcacc
    12481 agcaatggtt cctagaatat cttcaatggc atcattgtca atcaaattag ctagaacatc
    12541 agcctctcct aaattggtat ggagaaccag cataaaacct gctcgatcaa ctttcaaaac
    12601 ataaaaacgc acctcaggac taaatggtgt tgaaacgggt tcagacaaac tataatagag
    12661 tttgccttgt tcatctctca atttcaaaag gccaatttct cttaagtccc gagatagagt
    12721 tgcttgggtg accacaatgc cttctttttg aaggtgattt ttaatatctt cttgtgtgcc
    12781 aatatgctct gcttgaatga ttcgcttaat ctgctgttga cgctccattt tattcatgat
    12841 atatcacccc aattgtataa ttatcctctc aaattataac aaaaaaagtg tattatttca
    12901 tcttcgccca gctattttca gcaatttttt atcaaattca actagaattt cacttgaaaa
    12961 ctgttgaaaa gaaacctcat ttctcttttt ataacatttt cagaaaaaca acaacctcaa
    13021 gcaattcatt tccttgaaag tttcagtata atttatcaat cctctcctcg tttattttaa
    13081 agaaatatgt tagaataggg tgacaaacaa tttttaggag acgctgtatg gatactaaaa
    13141 ctctaatcgc aagtgaaata gctaaagttg ttccagaatt agaacaagat gctattttta
    13201 acttgcttga aaccccaaaa aattctgaca tgggagacct tgcctttcca gcctttagtt
    13261 tagcaaaagt ccttcgcaaa gcccctcaaa tgattgctag cgagttagct gaacagattg
    13321 atgagagcca atttgaaaaa gtcgtagctg tcggccctta catcaacttt ttccttgata
    13381 aagctaagat ttcctcacag gtccttgaac aagtgattac agcaggttca gactatgctc
    13441 agcaagacga aggtcaaggg cgaaatgttg ctattgacat gtcaagccca aacatcgcta
    13501 agccattttc aatcggtcac ctccgctcaa cagttattgg tgatagcctt gctaatatct
    13561 ttgctaaaat gggttatcaa cctgtaaaaa tcaatcacct tggtgactgg ggtaaacaat
    13621 ttggtatgct cattgttgcc tacaaaaaat ggggagacga agctgctgtt caggcacatc
    13681 ctattgatga actcttaaaa ctttacgttc gtatcaatgc tgaagctgaa attgatcaca
    13741 ccgttgatga agaagctcgt ggatggttcc gtaaattgga agatggcgac aaagaagcta
    13801 ctgaattatg gcaatggttc cgtgatgaaa gtttacttga gttcaaccga ctttatgacc
    13861 aattacatgt caccttcgat agttacaacg gtgaggcctt ctataatgac aaaatggatg
    13921 aggttcttga cttattagaa gctaaaaacc ttcttgtaga atccaaaggt gcccaagttg
    13981 ttaaccttga aaaatacggc attgaacacc ctgccttgat caaaaaatca gatggcgcta
    14041 ctctttacat tacccgtgac ctagctgccg cactttaccg taaacgcact tacgactttg
    14101 ctaaatccgt ttatgttgtt ggtaatgagc aagctgctca cttcaaacag ttaaaagcag
    14161 tgctaaaaga aatgggttac gattggtcag atgacatgac acacgttgct tttggacttg
    14221 tcacaaaagg cggtgctaaa ttatcaacac gtaaaggtaa tgttattctt cttgaaccta
    14281 cagttgctga agccattaac cgcgctgctt ctcaaattga agcgaaaaat ccaaatcttg
    14341 ctgataaaga agctgttgca cacgctgttg gtgtcggtgc catcaagttt tacgatctca
    14401 aaacagatcg tatgaatggg tatgactttg accttgaagc catggtttcc tttgaaggag
    14461 aaactggacc ttatgttcaa tacgctcacg ctcgtatcca atctattcta cgtaaagctg
    14521 actttacgcc atctgcaacc accacttaca gtttagctga tgctgaaagc tgggaaatta
    14581 tcaaacttat ccaagatttc ccacgcatta tcaagcgaac atcagataac tttgagcctt
    14641 ctattatggc taaatttgct attaacttag cacaaagctt taacaaatat tatgctcaca
    14701 ctcgcattct tgacgacaac tcagaacgtg ataaccgctt agctctctgc tatgcaactg
    14761 ctactgttct taaagaagcg cttcgcttac tcggtgttga cgcaccaaat gaaatgtaag
    14821 taataacatc actaaggctg tcacatttat gacagtctta gttttttata ttataataat
    14881 agcacaatat gttacaggag aatgccatgc catcagaaaa agaaatatta gatgctctta
    14941 gtaaagttta tagcgaacaa gtaatacaag cagatgatta ttttcgtcaa gcgatcttcg
    15001 agctcgccag tcaacttgaa aaagagggga tgagctcact actagctaca aaaattgact
    15061 cacttatcaa tcaatacatc ttaacacatc aatttgatgc tcctaaaagc atatttgatc
    15121 ttagtcgctt ggtaaaaaca aaagcttgcc attacaaggg aactgctata tcagctatta
    15181 tgcttggtag tttcttaagt gggggaccaa aataaaaata caggctgttt gacctgtatt
    15241 tttatttatc ataatttctt cggcgaattt taaaatcaga gctaataact tcgtccacat
    15301 caataatgga gataaaggca tctggatcca aaccagctaa aatacgtttg acttcccgaa
    15361 cttcagttgg gttcaaggtc acatacatga tattgtaatc attaccagag taagctcctt
    15421 gacctttcaa gtaagtcact ccacgattga tttcggttag gatagcttga gcggcagcct
    15481 ctgaatgctt ggtaataata atcattccac ggatagtgta accaccattt tgaaccacac
    15541 taatcatttg gctaaaaaca aagcttgcca ccaaagtata aagcatgtgt tttaaatcca
    15601 cataagtcaa cgaagctgtc aatacaagag catccaccaa aagcaaagtt tgacctagct
    15661 tagctccaaa tttttcttct gcaatgcgac caataatatc cgttccacct gtggttgcac
    15721 catagcgaaa aacaatgcca ctcccaatcc ctgaaaagag acctgccact acagccacca
    15781 acatcatgtc attttccaag ccaagttcga taggaacttt ttgccacatc cagataaaag
    15841 cggacatcag aactgttcca tagatggtta gagccaaaga acgcttccca aaaatctttg
    15901 cccctaaaat aaaaagtgga agattaaata agagagaaga gtaagcagga ttaatcccaa
    15961 aatgcgcgtg taaaatcaag gttattcctg ccacaccacc ttcagccaaa gcattagcca
    16021 tgttaaaatt aacaaaaccg aaggtataaa tggctacacc tagggcaatc aaaaacagtt
    16081 ttagtagttt agtcaattta tcgtgatgtg ccatccttta tcctcactat tcttcgcagg
    16141 caactctaat gctcatttat ttcctctttt tatggtattt gcttttaata gttaaaaatt
    16201 ctgggctatt gacttcatta acatcaaaat aagtgataaa ggcattggga tcaacctgag
    16261 ttaaaaaagc ttttaattcc ctaatatctc ttcgccccaa agccacataa atcacttttt
    16321 tacggcgccc agaataggct ccttcacctg ttagataagt aactcctctt cccaattcgt
    16381 ccatgatgaa ccgggcaatt ttctccgctt gttctgaaac gataaaaatg ccacgggctg
    16441 aataaccacc ttgttgtacc aagcgaacaa tttgaccata cataaagcta gcaatcagag
    16501 catacatcat ttgcggcagg gaaatataag tcaaagagac aatcatgaca aaaagatcaa
    16561 tgcctaaaag agcttgattc agctgtagtc ctaacttgtc ttctaataac ttagcaataa
    16621 tatctgctcc accgatggtt ccaccattgc gaaacacaag accgccacct agaccaccaa
    16681 taattccagc catcaaggaa acgactaaat tgtcatggtc caagtcaacg accaaaggaa
    16741 agcgttgaaa cagccacaca aaaaagtaaa gtgacaaaat acccattact gtgtaaacca
    16801 tggctctttt cccaaaacat ctagctccca acaagaccaa aggcaaatta atcaaatagc
    16861 ctatcaaaga aggatcaatc tcaaaaagtg ccttaccaac tagggtgaga ccagctaaac
    16921 cattggctgc aatgcgatta gccatgtaaa aataaacaaa agcaaaggcg tagatagcag
    16981 ctcctaaaat aataaagcaa aatttcttta actgtaaacg ttggtcaatt gacatgacac
    17041 ctcctgtgtt tttcttaatc atcttccaca aaacgaccga taattctaac attctcagcc
    17101 actgaaacaa aggcttgtgg gtctgttttt aacatcagcc atttgaattc tgggtattct
    17161 tcacatgtca aaatggtaat caaaactgct ttttgttcat gcttgtaagt cccttctgcg
    17221 tcgttgatac tggtcacccc gcgatgcaga cgtttatgga tcatatgaat cacacgctct
    17281 ggatggctgg taacaatagt tgcctgcatt ttcttttgct tggtgaaaat ggcatccgta
    17341 acacgacttg atacaaagat agagaccata gaataaaggg cgtactgcca gccaaataaa
    17401 atccctgcaa aggctaaaat tgcaccatta accatcaatg agagactgcc cacatccttg
    17461 cctgttcgtt ttctaagggt caaactgaca atatcagtcc ccccactaga gatacgtgat
    17521 ttgagacctg taccaattcc cattcccata accaaaccac caaaaatagc attgataaga
    17581 ggatcagtcg tcagcgtcac ttgaggtatg atttgaataa agaaagaact catgctgact
    17641 gtgataaagg taaaaatggt aaattgatgc ccaattttat accaagctaa aataagcaaa
    17701 ggaatattaa tcaaataaaa cgcaaaggcg atgggaaaat catagcctaa aagacgatga
    17761 ctaagagctg aaaaaacctg tgctagacca gttgctccac ttgaataaac atgcccaggc
    17821 tggaagaaaa aattcacagc aatactagag agaatgccat aaagcagaga agccgaaatc
    17881 ttctctgcat atttttctct tgaaatactt cttagagcgt ggagtaggcc aactttcttg
    17941 gcccctctac taatgacata tttaactttt ttcttatagg ttgttttctt aatcatgact
    18001 ttctatttga agactgagtt cttctaattg gttttcagaa acaagactag gtgcttgcgt
    18061 cattggatca gaggctttgt tatttttagg gaaggcaata acttctcgaa tattgtcttt
    18121 tccagcaagc aacatgacaa aacggtcaag accaattgcc aagccaccat gaggaggaaa
    18181 gccgtagtcc atcgcttcta gtaagaaacc aaattgggcg ttagcttcat cagcggtaaa
    18241 gccaagagcc ttgaacatgc gctcttgcat ctctttttga ttgatacgca ggctaccacc
    18301 accaagctcg taaccattta aaacgatgtc gtaagcaata gcccgtacct tagccaaatc
    18361 accctctaac tcatgagcag attctggcgt tggaagagtg aatgggtgat gagctgacat
    18421 ataacgtcct tcttcttctg accactcaaa cattggccaa tcaaccaccc aaaggaaatt
    18481 aaactgcgac tgatcaatca tgtcaaggtc cttggcaata cgattacgaa gggcacctaa
    18541 tgtattatta gcaacctcaa gagtatccgc tacaaataaa accaaatcat tctcagcaag
    18601 ttttaattgg cttgacaact ctgtctcaat agctgtcaag aacttggcta ctgggcctgc
    18661 caattgacca tttgtcacct taacccaggc aaggccttta gcaccaaatt gtttggcaaa
    18721 ctcagttaat ttgtcaatat ctttacgaga gtatcgatct gcatttccct tcaccacaat
    18781 cgctttgaca gctggtgctt ctgagaaaac cttgaagtca attcccttga cagttactgt
    18841 caagtcttgt aaaagcatct caaaacgtgt gtctggttta tcagagccat agctattcat
    18901 tgctacatcg tagctcatac gtgggaatgg tagggttaca tctatttctt ttgtctcttt
    18961 catcacttta gcaatcatgc cttcaacgat gtcttgaatt tcttgttcag aaaggaatga
    19021 cgtttccaag tcaacttgtg taaactcagg ttgacggtca ccacgtagat cttcatcgcg
    19081 gaaacatttc acaatttgat aatagcgatc aaagccagca ttcatcaata actgtttggt
    19141 aatttgcgga ctttgtggca aagcatagaa atgcccttgg ctaacacgac ttggtaccag
    19201 atagtcacgc gcaccttctg gagttgattt tgtcaacata ggtgtctcga catcaataaa
    19261 ctcaaggtca tcaaggtaat tacggataga atgagtcact tttgcacgta gcttgaagtt
    19321 ttctaacatt tctggacgtc gtaaatctaa gtaacggtaa cgtaaacgcg tgtcatcact
    19381 tacttcaacg tcatctttaa tttcaaaagg cgtcgtttta gctgtgttta aaatggtcaa
    19441 agcagatact tttaactcaa ccattcctgt cgccaactta tcattggctt gttgtcgagc
    19501 ttcaacaaaa ccttccactt caatcacata ttcactacgt aggcgttcag cagttgccat
    19561 cacatcactg gaaacttcct cagggttaat gactaattgc atcacaccct cacggtcacg
    19621 taaatcaata aagatgagac cacccaagtc acgacgacga ctgacccatc ctttcaatgt
    19681 gattgtcgtt ccaatgtgtt cttcgcgaac acgccctgca tacatgctac gtttcataaa
    19741 taaattccct aaaacaaaca gaatcctgtg aaacctgctc gtttccaatt cctttcacta
    19801 tataaatcaa ttaattcatt ccctactatt atagcaaaaa gcccttaaaa accccaccca
    19861 tttaaaaaga gtttttctaa agataaagca agaatactga gtcgtcagca ttttgacata
    19921 tcaggtgtcc tcacagaata ctaagaaagc taatcaatcc ccttacagaa aacaaacagg
    19981 tgcaactccc ttctaaaaag agaagttaca cctgttttag tgattatctt ctaaacaatc
    20041 atatcaacac aagcttatga cgacaataaa atcattaaga caataattgc tctgagatgt
    20101 tttcaaagtt ggtcatcata tcttcaaaac tgacttccac ttcttgacgg ctacgattat
    20161 tcttaatcac agctttgcct gcttcaactt cactttctcc taaagtcatt accaattttg
    20221 ctttaaaggt atcagctgat ttaaactgag ccttaatctt acggccaagg taatctcgtt
    20281 cagctgtaaa cccttgacgg cgaatggctt gaaccaattc taaagctttg ctattagcac
    20341 catcacctaa cacagcaagg taaatatcca tctctgtctc aattggcaag gtaatgccct
    20401 gcttttcaat aatcatcaag agacgttcta aaccaagacc aaagccaaat cctggagtct
    20461 caggcccacc aaaataacct actaaactat cataacgtcc acccgcacag atagttaagt
    20521 cagaaccttc cacagacgtg atgaattcaa aaatagtgtg gttatagtaa tcaaggccac
    20581 gcaccatatt ggtgtcaatc acataaggaa tgtcaagtgc ttcaagcata tctttgaccg
    20641 cctcaaagtg cgcctgactt tcctcatcta agtaatccaa aatagaaggc gctttttcaa
    20701 cggctagttt atcttctttt tctttagaat caagcacgcg cagtgggttt tcatccaagc
    20761 gacgttgact atctttagat aattgatcac gcattggtgt taagtagtca atcaaggctt
    20821 gacgataagc tgcacgactc tcaggactcc ccaaactatt taaatgaagg gtgacatcct
    20881 taatgcccaa tttttcaaag aggtgatagg ccatcgcaat ggtttcaaca tctgttgctg
    20941 gattagcagc gccgaaacat tctaccccaa tctggtgaaa ttcacgtaaa cgaccagctt
    21001 gaggacgttc gtagcggaac atggaaccga tataataaag tttgacgggt ttttggacct
    21061 ctggcgcaaa caatttattt tcaacataag aacgcaccac tggagctgta ccctctggtc
    21121 gcaaggtaat gtgacgatct cctttatcat agaaatcata catttccttc gtcacaatat
    21181 cagtcgtatc acccacagac cgcgagatta cctcgtagtg ttcaaacata ggagtacgaa
    21241 tttctccata attgtattgg ctaaacgtat cacgcgcaac agattccaca tactgccatt
    21301 tcgcagcatc cccaggcaga atatcctgag tcccttttgg tttttgaagt ttcataagtt
    21361 tacctctttg attagttttc tctttaaaca ttctaccatt ttttcttata ttttaaacaa
    21421 gcatatatca tttgctttag tttgatagta ttttcaccat gaaattgtaa aaataaaaag
    21481 ttttgtcaag taactttact ttacaaaaaa gatatgttat catagtgaag ttgatgaaaa
    21541 tcaaaactta cacatcgaaa ggaagaacaa gaaaatggca gtacctgcac gtcacacgtc
    21601 aaaagcgaag aaaaacaaac gtcgtacaca ctacaaattg acagctccat ctgtacaatt
    21661 cgacgaaact actggagatt actcacgttc tcaccgtgta tcccttaaag gatactacaa
    21721 aggacgtaaa atcgctaaag ctaacgaagc taaataatag aagggagata ccatgcgcgt
    21781 aaatattaca cttgaacata aagaatctgg tgaacgcttg taccttactt caaaaaacaa
    21841 acgtaacact ccagaccgtc ttcaattgaa aaaatactca ccaaaattac gtaaacacgt
    21901 tacatttaca gaagttaagt aaggggttca ctacacgtca ttagacgtca aaacattgat
    21961 tttaagcgat ttttgaattt tttgaagttc attacattgc aatataaatc aataaaaact
    22021 ctacctttta ctctaccttt tttagatgaa tctatcattt ggatattgaa gttaggccct
    22081 gttatcattt cgatggcagg gctttttaat atttggtaca ctaactcctt atctatattt
    22141 tggtggagct gaagttgaca tctataaagt ttaatgttaa tatacattca aaaatacatt
    22201 tgaatgaggt gttttatgat tcaaaatgtt gttacttcaa taatcctgta ttctgggaca
    22261 gccgtagact tacttattat cctaatgtta ttttttgcca aaagaaaaag cagaaaagac
    22321 atcattaaca tctatttagg acaatttcta ggctctgtta gtctaatatt gctaagtttg
    22381 ctttttgcat ttgtcttaga ttatattcct agtaaagaga ttttaggttt gctcggtttg
    22441 attccaattt tcctaggcct caaagttttg cttttaggag attctgatgg agaagctatt
    22501 gccaaagaag gtttgcgcaa agataataaa aacctgattt ttctagtcgc tatgattact
    22561 tttgcaagtt gtggtgctga caatattggt gtctttgtcc cgtattttac taccttaaat
    22621 ttagcgaatt tgatagtggc tttacttacc tttctagtca tgatttatct cttggttttt
    22681 tctgcccaaa aattggcaca agtcccttct gttggagaaa ttttggaaaa atatagcaga
    22741 tggtttattg ccgttgttta tttaggattg gggatatata tcctgattga aaacaacagt
    22801 tttgacatgc tatgggctgt gttaggctag gagaaaatat tatgaaaaaa gatagtatct
    22861 gccaagtgaa tgttataaat caacaaaatg ttacaaccgc aacgaactac cttgaaaagg
    22921 aaaaagtcca aaaatcactt cgtattttat caaagtttac cgataataaa cagataaata
    22981 tcatctttta tctccttact gttgaagaac tctgtgtctg cgatatagcc tgtttactaa
    23041 atctcagtat ggcatctgcc tcccaccatc ttcgtaaact agccaatcaa aacatcttgg
    23101 acactagaag agaggggaaa attatatatt attttataaa agatgaggaa atcagagatt
    23161 tttttaatca actaggataa caactatttt taccactttt ccatgtttat agagggctta
    23221 tctatgaaat tttttgatga aaattactca caagaaatac ctactcgtat caaatgttta
    23281 agaaaaaagt ataatttaaa gtaaagtgac ctaggtaatg caggtcaagt tagccaagtt
    23341 gaaaagggaa agcgacaagt taccgcttca atattgcttt atttgaatac tcaaaccgac
    23401 tcggactata aagaaattat ttttggagat attgccaagt ttgttgaaaa tatgttttac
    23461 cactacttca gctctatttt atttagagat ttggaaactg tagataaaag aatgtattct
    23521 ttttcggacg atgacttaat ttcaatacaa tccagctgtc ttagactttc caagacattc
    23581 gcaaatttca atattcaaag aaaaaatttt ttggcttctg atgaaactga aatggatact
    23641 tttcacaaga aagatgacat tgacattact gttggtgaaa aaagttataa cctcgctaga
    23701 agttttagaa ccagtaccat caatgaattg actgtcttta attttgaaga aatgtttgat
    23761 attatttggt tgatgttagg ggataattta attaaatcat ttgaagttaa tgtctgtggt
    23821 atcctatttg aattagacgg aaatggcata ccctcaacat ttaggcaaga aaacattgat
    23881 cccctcatca ataaatggtg gtatgataat gtttcgacag aaattatccc taacctaata
    23941 aaaaaactca aagaaaatcc actatttaat atcggattcc tggttgatga tattctagaa
    24001 cgaatgtata aagaaaatat cccaaaatct taccttacgt ccgtcccgtt agttatttcc
    24061 aaaaaagcaa ggtcaacttt atcgattaag tcaaacggaa gtcaaaaaat agataaatca
    24121 aaatttgcac agattttgtc cctttagaga cggatgactt acacattcaa cgtggattat
    24181 tcttcccttg gagaaagggg atttcagaac gcttggcgat tcctgattta gataaatttt
    24241 catctagctt acaacagggg aaaattcctg tcataaagca cttcgaactc aatttagata
    24301 aggggagtca ctgggctatt gctggaaatt caggttctgg gaaaccgtac gctctcactt
    24361 acttcttgag tgtcctgaaa cctatgtccg gcctaattat cattgaccca aaattcgata
    24421 cccctagcca atgggcaaga gaaaataaaa ttgccgttat ccacccagta gaaaaccatt
    24481 caaaatctga tttcgtttca caagtcaatg aacaattaaa ccagtgtgca actctcattc
    24541 agaaacgaca agctatcttg tatgataatc ccaatcatca atttacccat ctaactattg
    24601 ttattgatga agtcctcgct ctatcagagg gagtcaataa gaatatcaaa gaagcttttt
    24661 tctccctact atcacaaatt gccttgttgg gacatgctac taaaatccat ctattcttgg
    24721 gaagccagcg ttttgaccat aacactattc ccatttcagt gagggaacag ctaaacgtat
    24781 tattgcaaat tggaaatatc aatcagaaaa ccactcaatt cttatttcca gacctagatc
    24841 cagaggggat tgtcattcca acaggacacg gaacaggtat cattcaagtt gtcgataatg
    24901 aacactccta tcaagttcta cccttactct gtccaaccta ctatactaag cgaggtatcc
    24961 tatgaccact aaaacctact tcatgagaag cttcaatctc atattatact tactgcttat
    25021 tgctccaatc tgctatgtgt tcggttggct gttcaatctt gtttcagttc aattatctac
    25081 cgtcacattt gtcaatctgg atacggttaa aaacgttact gataccatta gttctgtatg
    25141 ttatggattc gctcttattt ctttatttct agccttatta tctggattaa agatgataca
    25201 acgcttgaat acagatagcc ttgggaacta ctgccaatct atttatcgca cctttctact
    25261 aagacacttc cttttccagc gtgaacgagt acagaaggta agcaactttg aacaccagac
    25321 cgttaccact atcaatccaa tccataacgg gtttaaccga gctgtacgta agtgtatcgt
    25381 agatatacga aaagatgcca ttactgtatt tctaaaagtt tctagagacc caacaggggc
    25441 aaaaaatcct aaaggatacg ggagtacaac tgaaagaaga gattgtcatc caacacacag
    25501 actattattt ctcttctcct gttcgagttc gtaaccaatt gtggtttatt ggtcaaaagc
    25561 gttaattcct tggggctgtg gcttgcgtta agcaaaagcc agacagcccc ttttttatat
    25621 tcttatctat catgacctat tggggttact acgacaacga aagttagaag ctgtgggtat
    25681 ttatttatct tgaaaagatt gctgaactaa cgcaataact actctaccat tcactctacc
    25741 ttttattaag caactatcaa taaatattga tacaataagg tttataacag ctaaaataga
    25801 aaatacttaa cctttacaga agtaaaataa ttgagatgag aaaagcctat gaaatgaacg
    25861 tttcaaaggc ttttttcgtg ttaaaaaaaa caaatgaaaa aacaaaatag agagtagggt
    25921 tattaacact gtcaatcctg tgtacgcaat aacaatccat gctttttgat aaggatattt
    25981 tgctttttct ttaatcttat caatctgatc taagctctca ccctttgcta tagcttcaag
    26041 ttatcggtaa gtctgaacat catatgtctt tttaaatcgc tcaattctta aggcgctacg
    26101 catgattatc aaccataaga tagcaaccgg accatatccc caccaaccta accaagacat
    26161 tataggataa atactgatag caattaggag tatcccaaat aacataatat gcgtattcct
    26221 atcacattgt ttaatctcct catccttaat aattaaagtc atttttggga tatcttctgc
    26281 tattagttgc tctaaaggaa cctgtaagga cttacttaac atcaaaagat tttgcaggtc
    26341 tggatagctt ttaccgtttt cccagtttga gatagtcttc ttgaaacata catcatttca
    26401 gcaagctctt cttgtgacat ctgtaatttt tttcaataat aaggtaactg gtttttaaag
    26461 cccataataa atctcctttt taatagctac tataatcctt cttcaataag tttactatca
    26521 aaagtctttg acctaagctt tttttcgttt atctttttga taattatcgt aaggtttaca
    26581 gtctttgtaa attttattta caactttgac aatcaaactt gacttgatca caaagacatg
    26641 gtgtttaagg aaaatatct
//
LOCUS       DAUGFP010000021        26486 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.021, whole genome shotgun sequence.
ACCESSION   DAUGFP010000021 DAUGFP010000000
VERSION     DAUGFP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26486
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.021"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            45..1241
                     /locus_tag="VH663_000914"
     CDS             45..1241
                     /locus_tag="VH663_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEQ8174126.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTLCVFIVLYILGLMV
                     ALRLKK"
     gene            1307..2014
                     /locus_tag="VH663_000915"
     CDS             1307..2014
                     /locus_tag="VH663_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEQ8174127.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQTTGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLRVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADIVLPD"
     gene            2077..3132
                     /gene="prsA"
                     /locus_tag="VH663_000916"
     CDS             2077..3132
                     /gene="prsA"
                     /locus_tag="VH663_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ8174128.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            3519..6137
                     /gene="alaS"
                     /locus_tag="VH663_000917"
     CDS             3519..6137
                     /gene="alaS"
                     /locus_tag="VH663_000917"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEQ8174129.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRN
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAETV"
     gene            6477..6692
                     /locus_tag="VH663_000918"
     CDS             6477..6692
                     /locus_tag="VH663_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8174130.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            6689..7450
                     /locus_tag="VH663_000919"
     CDS             6689..7450
                     /locus_tag="VH663_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEQ8174131.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEEDDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVFSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            7469..8164
                     /locus_tag="VH663_000920"
     CDS             7469..8164
                     /locus_tag="VH663_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ8174132.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIVSTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
     gene            complement(8256..9413)
                     /locus_tag="VH663_000921"
                     /pseudo
     CDS             complement(8256..9413)
                     /locus_tag="VH663_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558257.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(9509..10822)
                     /locus_tag="VH663_000922"
     CDS             complement(9509..10822)
                     /locus_tag="VH663_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEQ8174133.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVTLT
                     SLWSQRCNLSKADQKLLLACASSAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIWSVLAGLILSLFAHAYKHLLEHLPK
                     ADAKSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVAKA
                     VAISLVFASGAKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLMIIPLALANLIFYFSYQFYRFILK"
     gene            complement(10797..11756)
                     /gene="nrdF"
                     /locus_tag="VH663_000923"
     CDS             complement(10797..11756)
                     /gene="nrdF"
                     /locus_tag="VH663_000923"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ8174134.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINDIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            complement(12089..14248)
                     /gene="nrdE"
                     /locus_tag="VH663_000924"
     CDS             complement(12089..14248)
                     /gene="nrdE"
                     /locus_tag="VH663_000924"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ8174135.1"
                     /translation="MSLKDIGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFITELDSIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYANGSYFDKYVTGHFVPKSNLVKSLFKDHFIPQAEDWKALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(14268..14486)
                     /locus_tag="VH663_000925"
     CDS             complement(14268..14486)
                     /locus_tag="VH663_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEQ8174136.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
     gene            14879..15142
                     /locus_tag="VH663_000926"
     CDS             14879..15142
                     /locus_tag="VH663_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEQ8174137.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            15147..16880
                     /gene="ptsP"
                     /locus_tag="VH663_000927"
     CDS             15147..16880
                     /gene="ptsP"
                     /locus_tag="VH663_000927"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEQ8174138.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            17065..18492
                     /locus_tag="VH663_000928"
     CDS             17065..18492
                     /locus_tag="VH663_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ8174139.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALISGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLVLAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAIKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
     gene            18587..19861
                     /locus_tag="VH663_000929"
     CDS             18587..19861
                     /locus_tag="VH663_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ8174140.1"
                     /translation="MKKLNVILVGLLSILILSLAIVFINRWKLNEDSQRIVLAEKKKN
                     TSDLVIKAVKHIKKDQKDYYYFSPIKQADDFFVDNLPVSLYKKKNSDKELILVKPKLQ
                     SSHLRSVNTLTISKIVYQKKFFHLAKKSEKVISTYHVTDDLKPFQVRDLVSGHLERIQ
                     EEVEKKYPNAGFNSDKYNGLKESNSLLSDGFEVKSGNLIFDKKLTIPLTTLFDVINPD
                     FLANSDRAAYDNYRTYKEQHPKKLVALTFDDGPDPTTTPQVLDILAKYQAKGTFFMIG
                     SKVVNNENLTKRVSDAGHEIANHTWDHPNLTNLSVSEIQHQVNMTNQAIEKACGKKPR
                     YLRPPYGATNATVQQSSGLTQMLWTVDTRDWENHSTDGIMTNVKNQLQPGGVVLMHDI
                     HQTTINALPTVMEYLKAEGYECVTVSELYAHQ"
     gene            complement(19971..21056)
                     /locus_tag="VH663_000930"
     CDS             complement(19971..21056)
                     /locus_tag="VH663_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ8174141.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            21154..21780
                     /gene="udk"
                     /locus_tag="VH663_000931"
     CDS             21154..21780
                     /gene="udk"
                     /locus_tag="VH663_000931"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEQ8174142.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            21860..22123
                     /locus_tag="VH663_000932"
     CDS             21860..22123
                     /locus_tag="VH663_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEQ8174143.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERKLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(22176..22985)
                     /locus_tag="VH663_000933"
     CDS             complement(22176..22985)
                     /locus_tag="VH663_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEQ8174144.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     TEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            23130..23627
                     /locus_tag="VH663_000934"
     CDS             23130..23627
                     /locus_tag="VH663_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEQ8174145.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKESHLIGVLDLDSSLVADYDEVDQEYLEAFVDLLLEKTTFTFNM
                     FGVKN"
     gene            23627..25297
                     /gene="dnaX"
                     /locus_tag="VH663_000935"
     CDS             23627..25297
                     /gene="dnaX"
                     /locus_tag="VH663_000935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEQ8174146.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISMLALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAQKEQILSQANLSGELISEIETLKNELAQLKQQLSQLQSRPD
                     SLARSDKTKPKTTSYRVDRVTILKIMEETVRNSQQSRQYLDALKNAWNEILDNISAQD
                     RALLMGSEPVLANSENAILAFEAAFNAEQVMSRNNLNDMFGNIMSKAAGFSPNILAVP
                     RTDFQHIRKEFAQQMKSQKDSVQEEQEVALDIPEGFDFLLDKINTIDD"
     gene            25392..25595
                     /locus_tag="VH663_000936"
     CDS             25392..25595
                     /locus_tag="VH663_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEQ8174147.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKAANSPKKKD"
     gene            complement(25570..26481)
                     /gene="birA"
                     /locus_tag="VH663_000937"
     CDS             complement(25570..26481)
                     /gene="birA"
                     /locus_tag="VH663_000937"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HEQ8174148.1"
                     /translation="MSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQGIQIDSLKHK
                     GYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPRLYLAPSQEAA
                     KGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISRLTGIDTEIKW
                     VNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIAQKAGSLFTEK
                     PTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEKRAIAIDLTDQ
                     GHLIVQFDNGDLQTLRSGEISLSSWEN"
BASE COUNT         8367 a         4583 c         5469 g         8067 t
ORIGIN      
        1 ttttttaaaa aataaacgtt cgtataataa aaaaggagtt ttttatggaa aaaacaaaac
       61 gttatatcat tgcaacagca gggatacttc tccacttaat gttgggatcg acctatgctt
      121 ggagtgttta tcgtaatcca attttacaag agaccggttg ggatcaagct cctgtcgctt
      181 ttgccttttc tttagctatt ttttgtttag gtttatctgc tgcttttatg ggaaatttag
      241 ttgaacaata tggtcctcgt ctaaccggaa cggtgtcggc tattctttat gcttctggaa
      301 atatgctgac tggtttagcg attgatcgta aagaaatttg gcttttatat attggttatg
      361 gtgttattgg tggcttagga cttggagcgg gatatatcac accgatatca actattataa
      421 aatggtttcc agataaaaga ggcatggcaa cagggtttgc tattatgggg tttggttttg
      481 cgtctttatt aacaagtccg attgcccagt ggctaattga aacagaaggt ttggtagcaa
      541 cattttacct tttagggctg atttatctaa tagttatgct ttttgcttct caattgataa
      601 taaaaccaac tgctgctgag atagctatac tagataaaaa aagattacaa aataacagtt
      661 atttaatcga aggaatgacc gctaaagaag cacttaaaac aaaatcattt tattgtcttt
      721 gggtaatttt atttatcaat atcacatgtg gcttaggact tatctcagtt gttgcgccaa
      781 tggcacaaga tctaactggt atgagtccag aaatgtcagc aattgtggtt ggtgctatgg
      841 ggatttttaa cggtttcggt cgtttagtgt gggctagttt gtcagactat attggacgcc
      901 gtgtgacagt aatactttta tttttagtaa gcataattat gactattagt ttaatatttg
      961 cacatagttc cctaattttc atgataagca ttgccaccct tatgacttgt tatggtgcgg
     1021 gtttttctct cattccgcct tatttaagtg atctttttgg ggcaaaggaa ctggctacat
     1081 tacatgggta tatattgaca gcttgggcaa tagctgcatt aactggacct atgttattat
     1141 ctatcactgt tgaatggact cataattatc ttcttacctt atgtgttttt attgtcttat
     1201 acattttagg attaatggta gcattaaggt taaagaaatg atgtcctata aaaaatttaa
     1261 aagtaaaccc tagcaaagcc tagggttttt ttatataatt aggtttatgg ttaaatcata
     1321 cagtaaaaca gcaaatcata atatgcgtcg tcctgttgtc aaagaagagc ttgttcatta
     1381 tatgcgaaca cggcagaagc aaaccacagg ttttttagca gagttagagc aattcgcaag
     1441 acaagaaaac attcctatta ttcagcctga ggtcgtggct tatttcaggt ttttattgca
     1501 aagcttacag ccaaaacaca ttttggagat tggcacagcc attggttttt ctgcgctttt
     1561 aatggctgaa aatgcaccag atgcgacgat tgtgactatt gatcgtaatc gagagatgat
     1621 cgattttgct aaagcaaatt ttgctaagta cgacagtcgc caacagatcc gcctgttaga
     1681 aggagatgca gcagatatat tatctacact tgaaggaaat tttgactttg tgtttatgga
     1741 ttccgccaaa tccaaatata ttgtctttct accagagatt ttaagactgt taagagtcgg
     1801 tggtgtagtc attttggatg atgtctttca aggaggtgac attacaaaac cgatcgaaga
     1861 cattcgacga ggtcagcgta ctatttatcg tggcttacag tctttgtttg atgccacctt
     1921 aactcatcca aacctaacaa ctagcctggt ccctcttagt gatggattat taatgattcg
     1981 taaaaatcaa gcagatattg tattaccaga ttaagtaaca ttgataaatt tatgttataa
     2041 taaattagtt aaagataaaa aggagctcag aagtaaatga aaaactcaaa taaactcatt
     2101 gctagtgttg tgacattggc ctcagtgatg gctttagcag cttgtcaatc aactaatgac
     2161 aatactaagg ttatttcgat gaaaggtgat acaattagcg ttagtgattt ttacaatgaa
     2221 acaaaaaaca cagaagtatc gcaaaaagcg atgctaaatc tggtaattag tcgtgttttt
     2281 gaagctcaat atggtgataa ggtttcaaaa aaagaagttg aaaaggcgta tcataaaaca
     2341 gctgaacagt atggcgcttc attctctgct gctttggcac aatcaagctt gacacctgag
     2401 acttttaagc gtcagatccg ctcttcaaaa ttagtagaat atgcggttaa agaagcagct
     2461 aaaaaagaat tgacaacaca agaatataag aaagcatatg aatcttatac tccaacaatg
     2521 gcagtcgaaa tgattacttt agataatgaa gagacagcta aatcagtctt agaggaacta
     2581 aaagccgaag gcgcagactt tacagctatt gctaaagaaa aaacaacaac acctgagaaa
     2641 aaagtgacct ataaatttga ttcaggcgcg acaaatgtac cgactgatgt cgtaaaagcg
     2701 gcttcaagtt tgaatgaggg tggaatatca gacgttatct cggttttaga tccaacttct
     2761 tatcaaaaga agttttacat tgttaaggtg actaaaaaag cagaaaaaaa atcagattgg
     2821 caagaatata agaaacgttt gaaagctatc attatagctg aaaaatcaaa agatatgaat
     2881 ttccaaaaca aggttattgc aaatgcattg gataaagcta atgtaaaaat taaagacaaa
     2941 gcttttgcta atattttggc gcaatatgca aatcttggtc aaaaaactaa agctgcaagt
     3001 gaaagttcaa caaccagcga atcatcaaaa gctgcagaag agaacccatc agaatcagag
     3061 caaacacaga catcatcagc tgaagaacca actgagactg aggctcagac gcaagagcca
     3121 gctgcacaat aagtaggatt ttattgacta atcagttaaa aaaacttata ataatttagt
     3181 taagaattgt ttatcaagga ttagatacag aaaagtgacg gttgctgcga gtcattgata
     3241 attagataaa catgctactt aactttacaa caattgatat cgtaaattaa gaatgacaag
     3301 ttcattgaat agaggtggta ccgcggtttt cgccctctgt gagatggact tgttttgtat
     3361 tgaggactat ttgaaatcaa cgaaaggaat agagtagact aggctgccag gcttatagct
     3421 gatctcctag caagaaagac gagctgtgtc aactggtgtg attacattac tagcttcagc
     3481 ataacactgt ttttcttact ctttgtatct aaatttttat gaaagaatta tcgtctgcac
     3541 aaatccgcca aatgtggttg gatttctgga aatccaaagg acattgcgtt gagccttcag
     3601 ctaacttggt tcctgtgaac gacccaacgc ttctttggat caactcaggt gttgcaacct
     3661 tgaaaaaata ttttgatggt tcagtgattc cagaaaatcc acgtattacc aatgcacaaa
     3721 aatcaattcg tactaatgat attgaaaatg ttggtaaaac agcacgtcac catactatgt
     3781 ttgaaatgct tggtaacttc tcaattggag actatttccg taatgaagct attgagtggg
     3841 gatttgaact cttgacaagt ccagactggt ttgatttccc taaagacaag ctctacatga
     3901 cttattaccc agatgacaag gattcgtata accgttggat tgcttgtggc gttgaaccaa
     3961 gtcacttggt gccgatcgag gataacttct gggaaatcgg tgctggtcct tcaggtccag
     4021 atacggagat tttcttcgat cgtggtgaag atttcgatcc agaaaatatc ggacttcgcc
     4081 tcttggctga agatatcgaa aacgatcgtt acatcgaaat ctggaacatc gttctctcac
     4141 aattcaatgc tgacccagcc gtaccacgtt cagaatacaa agaattacca aacaaaaaca
     4201 ttgatacagg tgctggtctt gaacgtcttg cagctgttat gcaaggggca aaaacaaact
     4261 ttgaaactga ccttttcatg ccaatcatcc gtgaagtaga gaagttgtca ggtaaaactt
     4321 acgatccaga tggcgacaac atgagtttca aggttatcgc tgaccacatc cgtgcgcttt
     4381 catttgctat cggtgatggt gcgcttcctg gaaatgaagg tcgtggttac gttcttcgtc
     4441 gtcttctccg tcgtgcggtt atgcatggcc gccgtcttgg catcaacgaa actttccttt
     4501 acaaattggt tccgactgtt ggacaaatca tggaaagcta ctacccagaa gtgcttgaaa
     4561 aacgtgattt tatcgagaaa atcgttaaac gtgaggaaga aacatttgct cgtactatcg
     4621 atgcaggtag cggtcactta gattcattgc ttgcgcagct taaggctgaa ggtaaggata
     4681 ctcttgaagg taaagatatc ttcaaacttt atgatactta tggattcccg gttgaattga
     4741 cagaggaatt ggcagaagat gcaggctaca agattgacca cgaaggcttt aagtcagcca
     4801 tgaaagaaca acaagaccgt gcgcgtgcag ctgttgttaa gggtggttca atggggatgc
     4861 aaaatgaaac cctagctggt attgttgaag aatcacgatt cgaatacgac acatatagtc
     4921 ttgaatcaag tctttcagtc atcatcgctg ataatgaacg taccgaagct gtttcagaag
     4981 gtcaagctct tcttgtcttt gctcaaacac cattctatgc tgaaatgggt ggacaggttg
     5041 ctgacacagg tagaatcaaa aatgataagg gtgacacagt tgctgaggtt gttgatgttc
     5101 aaaaagcacc taatggtcaa cctctacaca ctgtaaacgt tttggcatca ctttcagttg
     5161 gaacaaacta cacacttgaa atcaacaaag agcggcgttt ggctgttgag aaaaaccaca
     5221 cagctactca cttgctccat gcagctcttc acaatgttat tggtgaacac gcaactcagg
     5281 ctggttcatt gaacgaagaa gaattcttgc gctttgattt tactcacttt gaagcagtaa
     5341 gcaatgagga acttcgtcac attgaacaag aagttaatga gcaaatttgg aacgctctta
     5401 caatcacaac gactgaaact gacgttgaaa ccgcaaaaga gatgggagca atggcacttt
     5461 ttggtgagaa atatggtaaa gtggttcgtg tggttcaaat tggtaattat tctgttgaac
     5521 tttgtggtgg aactcactta aataattctt cagaaatcgg tctcttcaag attgtcaaag
     5581 aagaaggtat tggttcaggc actcgtcgta ttattgcagt tactggtaga caagcttttg
     5641 aagcttatcg taaccaagag gatgccctaa aagagatcgc tgctactgta aaagctccgc
     5701 aattgaaaga tgcagcagct aaagtacaag ctcttagcga ctcacttcgt gatcttcaaa
     5761 aagaaaatgc agaacttaaa gaaaaagcag cagctgcagc agctggtgat gtctttaaag
     5821 atgttcaaga agctaagggc gtgcgcttca ttgctagtca agttgatgtt gcagatgcag
     5881 gggcacttcg tacatttgct gataactgga aacaaaaaga ctactctgat gtgcttgttc
     5941 tcgtagcagc tattggtgag aaggttaatg tccttgttgc aagcaaaacc aaagatgtcc
     6001 acgctggtaa catgatcaaa gaattggcac caattgtagc aggtcgtggt ggaggtaaac
     6061 cagacatggc tatggcaggt ggtagcgatg caagtaaaat tgcagagctg ctagcagcag
     6121 ttgctgaaac agtgtaaacc cataaaaaac caagttattt agaacttggt tttttatatt
     6181 tgaaatgact attatcgttg tcaaaaagaa aaagatatgt tacaatgata gtaacaaata
     6241 gacatgagga ggtgttagag aaaatgacac atggtatcaa acttaagagg cagatttgct
     6301 tatgggcaag tatcctgact cttttcattt taagttatca tcttatcagg gtaatctggt
     6361 ttggagtacc cttgagttat ctggttttgg gctttacagg actagccttg ctgttgaata
     6421 ctttttctta ttttagaaag taagttataa gttaaagaag aatgaaggag atggcaatgg
     6481 aggttattct taaaaatcgg ctcaaagaat tacgagccag agatggaatt aatcaaacgg
     6541 aaatggctaa actggcagga gtatcgaggc agaccatcag tctcattgag cgtaacgagt
     6601 acaccccatc agttataatt gccatgaaaa ttgcaaaggt atttcaggaa ccagttgaag
     6661 aggtgtttcg tctagtggag gtcgaggaat gaaaaagaag aagaatagtt ttttacgctt
     6721 gttaaaaatg ttattgctat caagtttggc gggagggatt ataggtggca tggttggagc
     6781 ctttttaggt taccatggag agagattaga tcagctcacg ttcctaaaag atgatgtgat
     6841 taacctgatt atcttattaa atcgtctagt agttgtcaca ggcctcacac tttcttttgt
     6901 ttttttaacg caactgaaaa aagagacagc tgtttataac acaattgaag aggatgatta
     6961 ttctgaaaat ggttaccgtc agctgaataa aaagcatgct tataccatgt tattgattgc
     7021 cgtagcttct attttatcca tgtgcaatgt gctacttggt ctgaccttaa ctaatgattc
     7081 acagcatgcc atgctggcca ttccgcttct ggatatcctt ttattattaa tggtcattcc
     7141 ttttcaagct ttagccatga aacgttacaa tgccattcgg ggaaccgatg ttccttattt
     7201 tccaaatttg aaagaattaa aacataatat catggctcta gatgaggcag agttacaagc
     7261 ctatcataag acatcgtttg aaagtgtgtt ttcattaaat ggagtcatca tcccttcctt
     7321 gtatgtgatt ttattttttg tctatctatt cactggtcaa gtggagttaa cagccatctt
     7381 agttttggtg ttaattcagc tctatttact cgttaagagt gcaacaatga ccagacaatt
     7441 ttaccgttag cctaaaagga gactgtttat gaaaggattc attaattatt taaaaatagc
     7501 agtgctcatt attctggcta tggttttcaa tgtccttccg atgattttat tgcaaaagca
     7561 acacgatatt cctatggtac ttaattgggg aattggtatt ttctacttgg ttattgttgg
     7621 aagtgtcctt attgtattat ggcgtcttta tcaagctaag caagacaccc ctattaaaca
     7681 gcaaaaaatg agattggttg actggggtta tttagcatta ttttggttaa tcatccgtgt
     7741 gatagctatt gtaagtaccc ttgttaacca gctatggtct ggtcaacaag tgagtgctaa
     7801 tgatgctgca atacatacct tagctagact tatcaaaggt ggtttcccgc tttatactgc
     7861 cctatttgta cttgtgatag cttttatcgc tcctattatg gaagaactag tctttagagg
     7921 atttcctatg attgatctct tcaaaggaaa atcacttaag gtggcaggtt tagtgacctc
     7981 tcttgttttt gctttaccac atgccaccaa tagtgttgaa tttatcatgt acagctgtat
     8041 gggcattttt ctctttgttg cctatcaaag acgaggaaac ttaaaagatg ctatcttgtt
     8101 acatattttt aataacttga ttgaagtcat tttgttaatg tcaataggct taggagtcat
     8161 ataataaaaa gcttcggatg gatgaactgc accccaaaag ttggacaaaa aaactaacat
     8221 ttggttggac ttgtcaagaa aaaatagaca taaagttaag ctaatgagta actatttttt
     8281 tcaaattcaa caggtgagag ataccctaaa gctgaatgca gacgtttggg gttatagtaa
     8341 gtctcactat atttaaaaat ctcaagctga gcttgctcaa tagttgcgaa atgagcatca
     8401 ttgacaagct ctctttttaa tgttttataa aaggattcca tgagtgcatt atcgtatgga
     8461 ttaccttttc gactcatcct ggacttacat tttttctgtc taagtaggtc ctgaaattct
     8521 gctccggtat attgagaacc ctgatctgta tggacgatta ccccttcttt aggcttttct
     8581 ctatttataa gcttgattga aagcttctgt taccaacttg tcttgcatac gttagcccat
     8641 tgcccaacca ataacctttt tactataaac gtctataaag acagaaacat aaagtattcc
     8701 ttcttgtgta ggaatgtagg ttaggtctcc tagccataat ttattcttac cagtcgcttg
     8761 gaaacattga ttaacaagat tgggacgagt taatgaagaa cgtcgacggt tatagtattt
     8821 atattgatat cgacttcctt tagcatagag acctagctga tgaaggagtc taccaacacg
     8881 tttatgattg acatggatgc cacgtctaca aagttcttgg gtaattcgta cgcttccgta
     8941 acgctcttta tgttcataga aaatggcttt aatcatttcc ttcaaagctt cattctcaac
     9001 atgccttgaa gagggacgac gttttaaata agcatagaaa cctgaacggg agacttgaag
     9061 agtttgacag gtgtgatgac tattcaagct aacactattt tccttcagaa actgaaattt
     9121 tatttccggt ttggcttcaa gaagacctgg aactttttta gaagagctaa ttcctcctgt
     9181 agcagtttgt tctctttttc aagttttttt atctcaaatt gagcatgacg aagtgcgctc
     9241 ccatgtcctg ggaacgcact ttctccatat ttttcatatt cttgaatcca tcgataaaga
     9301 ctattgggat gaatttctaa agttgaacta accattttta caggctgctc ttcctcaagg
     9361 atgagtttta cagcagagag tttgaaggct ttatcaaagg tttttcttgg cataatgact
     9421 tttcctcgtt tccacttaat tttgtgtcta ctttagtata ccaagtccaa ttcatccgaa
     9481 gctttttatt aaaaaagtaa ttaactaatc atttaaggat gaaacgataa aactgatagc
     9541 taaaataaaa aatgaggtta gctaatgcta aagggataat cattagtaac gattggcctg
     9601 taatttcaac cagaaaaact ggtgccgcca agggtatctt attgatcaca cctaaaaaga
     9661 ttgttgcacc aaccataatg gctaacgtat ttgatagaga caagggaata aggtactgac
     9721 taaggatagc tagtagtaag ccactagcac ctccaagcat catagaagga gcaattttac
     9781 ccccttttgc tccagaagca aaaactaaag agatagcgac agctttggca acaagtagcc
     9841 agctaatata gctcagatgt ggttcttcat ggagaaaaaa gagtaatcca gctttgccat
     9901 ttcctaaaat ctctgggaag aaaatgctga gtcctgcgat aaggctaaaa gcgatgagaa
     9961 ctttcggaat aaaccactga cttttggcat cagccttagg aaggtgttcc aaaagatgtt
    10021 tgtaagcgtg agcaaagagg gaaaggataa gaccagccag gacggaccaa ataagggttc
    10081 ctagcgtcca atgttgctga ggcattaggt attgaatctc atgtcggcct tgtaataaag
    10141 caactgtttc cacagccaca tagctcgtta ggcaggcagc gtatatattt ttaagggacc
    10201 aacggttaag aatagcttcc aagataaata aaatagtggc taagggagca ttgtagacag
    10261 caccaagggc agcgctagaa gcgcaagcta ataaaagttt ttgatcggct tttgacaagt
    10321 tgcatctttg gctccaaaga gacgtcaagg taacagcgac ttcccgtgaa gctccttctc
    10381 ggccgacagg agctcccata gatacggtcg ttaattggag ccaaccatgc caaaactgtg
    10441 tccagggact aaatgagatg tcatcttgaa tgatttgctg aatagattga atgtcctttc
    10501 cttttttagc caataggtgc cacccaagac ctgcaagaag tccagcaaag agaagtgaaa
    10561 gagctcgtct ttgtggtgga acgctagcaa tcatagtgct aaaagaacct tgactgaatc
    10621 caaaactgag ggactgaatc aggtgaatgg cttttgttaa gataaaagca acaaggccag
    10681 ctgttaaacc tgttaaaaag aggtaaaagg tcaagagtaa aaagtgtcgt ttcataagac
    10741 tattataaca aaagttaaga aaagagactg atagattatc agtctcgtga gcactattag
    10801 agtccataat tataatcgtc atcagacata gcttctacag aaccaagaag atagccattc
    10861 cctacctgtg agaaaaagtc atggtttgat gttcctgttg aaataccatt cataacaatt
    10921 gggttaacat cgtttgcagt atctgggaat aaaggatctt gtcctaaatt cataagagct
    10981 ttgttagcat tgtagcgtaa aaaggtcata acttcctcag tccaccctac gccatcataa
    11041 agcgttttgg tgtatttttc ttcattttca tacagctgat aaaggaggtc atacatccaa
    11101 tcacgaaaat tctcttgctc atcttctggt agttcgttaa aaccaagctg gaatttgtaa
    11161 ccgatataag taccatgtac agattcatca cgaataatta gtttaatgat ttcagcaaca
    11221 tttgctagct tattatttcc caaatagtaa agaggggtga aaaagccaga ataaaaaagg
    11281 aaagtttcga gatatgttga ggcaactttc ttttgtaagg catctccatt agcgtaaatg
    11341 tcattgataa tacgtgcttt ttcttgaaga aactcattat tattagtcca ctcaaagatc
    11401 tcttcaattt cttttttggt gtttaaggtt gaaaagatag acgaataaga tttagcgtga
    11461 acagattcca tgaattgaat attgtttaag acagcttctt cgtgaggagt gcgaacatca
    11521 gcacgaatag cttcgacacc agtttctgat tgcatggtat caagtagggt taatcctcca
    11581 aaaaccttgc caacaagatc tttttcttga agagaaagtt tgcgccaatc gtctaagtca
    11641 tttgataaag ggatacgggt atcaagccaa aattgttcgg tcaacttttc ccaagttgac
    11701 ttgtcaatga cgtcttcgat ttcattccag ttaattgctt cataatatgt agtcatagaa
    11761 aaatgatctc ctaaagagta gtaaagtgcg ttaaggatat cgagataaac cagtagaacc
    11821 gaccaaagtt ctagtggtaa taataagttt aaatagattc aaaagggtaa cgaatgagtt
    11881 agtgagtggt ttaggcagat cgcctaaagc gactgccgta gaagtaatag atgctttagc
    11941 atcattaggg catatcgagg aattacgaca cggagtaagg aaagtcgata atactttcct
    12001 tacatagtta gtgagtggtt taggcagatc gcctaaagcg actgccgtag aagtaataga
    12061 tgctttagca tcattacttc ttaaccaatt aaatgacaca agattcacat tggtttgcgc
    12121 ccacttcttc cccatcatcc gtaaaggtac ggatatagta gatagattta atgcctttat
    12181 tgaaagcgta gtttcgtaag atggataaat cacgagtggt ttgtttgctt tgtgttttcc
    12241 actcataaag ctccataggc aactcactac gaaggaatag agttaatgac aagccttggt
    12301 ctacatgttc ggtcgcagcg gcatagacat caataacttt gcgcatgtcc atatcgtaag
    12361 cagatgtata gtaaggaatg gtatccgtag acaaaccatt tgcaggatag tagattttac
    12421 caattttctt ttcttgacgc tcttcgatgc gttgtgtgat tgggtgaata gaagcagagc
    12481 agtcattgat ataagaaata gagccatttg gagcaactgc tagacggttt tgatgataaa
    12541 gaccatcttt ttgaacggcg tcgcgaagag ctttccaatc ctctgcttga ggaataaagt
    12601 gatctttaaa gagacttttg accaaattag attttggaac aaagtgtcct gtaacgtatt
    12661 tatcaaagta actaccatta gcgtacttag agttctcaaa gccaacaaag gtagtttggc
    12721 gctcacgagc gatgttattg gattcgacca aggtccaata attcaggagc ataaagtaaa
    12781 tatcagtaaa ctcgatggat tctggactgc catattcaat atgatgttga gcaaggtaag
    12841 aatgtagtcc catagctcca aggccaaaag tatgagcttg gctattgcca tgtttaatgg
    12901 ttggaacagc ttcaatgctt gatgaatcag taacaaaagt tagggcacgt gtcatggtct
    12961 taatagaacg gccaaagtct ggtgaggtca tcatgttcag gatattagtg gaacctaagt
    13021 tacatgaaat atcagttccc atttctacaa actcttgcgc atcattgata aggctaggtg
    13081 tttgaacttg taaaatttca gaacacaagt tgctcatgat gatttttcca tcgataggat
    13141 tagctttatt agctgtatca atattgatga tataagggta accagattct tgttgtaatt
    13201 ttgaaatctc tgtttcaaga tcacgagctt taattttagt cttagtaatt ttagggttcg
    13261 ccactaactc atcgtacata ttggtaatgt cgagatagtt aaagggaatg ccatattctt
    13321 tttcaacatt gtaaggacta aagagataca tgtcctcgtt tttacgagct aattcgtaga
    13381 atttatcagg aacggtaatc cctagtgaca aggttttaac acgcaccttt tcatcggcat
    13441 tttctttttt agtagataag aaagcaatga tatcaggatg aaaaacattt aggtaaacaa
    13501 caccagctcc ttgacgttgc ccaagttgat ttgaataaga aaaactatct tcaaataatt
    13561 tcataacagg aacaactcct gaggctgcac cagcatagcc tttgattggt gcgccagctt
    13621 cacggaggtt agacaaggta atcccaacac ctccaccaat acgggataat tgcaaggcag
    13681 agttaatgga acgtccgata gaattcatgt catcagttac ttgaatcaag aaacaagaga
    13741 ccaattcacc acgacggctt cgaccagcat ttaaaaagga aggagtagcc ggttggtaac
    13801 gttggttaat catttcaacg gctaaatctt ttgctaagtc ttcttgacca tctgcaaaat
    13861 acaaagcatt aaacaagaca cggtcttcaa ggttttctaa ataatgctct ccatcatttg
    13921 tttttaaggc gtattgctgg tagaacttgt aggctgccat aaatgattta aagcgaaaat
    13981 tttctgattt gattatgcta tctaattcag taataaattc aggacggtat ttctgaataa
    14041 aagctgattc aatgtaatca tttgagatta aatactcaat tttttccgta atggaagtaa
    14101 aagacatggt gtttggcagc acattttcag cgaaaaaagc ttttaaagct tctttgtctt
    14161 tatgaagtgg aattttacca ttaacaggac ggttaatttc attatttagg cgaaaatatg
    14221 aaatatcgcc aatatctttg agactcatat tatttttcct tgtcttatta aatcaattct
    14281 tttaactttg caggttggaa gccagaaaat acgaggttat ctgcttcgat aacaggagca
    14341 gatgtgaagc caagactttt cacataatcc actttttcag gatgttcatc gatattaatt
    14401 tcctggaagt tggctccgtg ttgttctaaa aatttcttag tcattttgca ctgcatgcaa
    14461 ttatttttgg aatatactgt aatcatgtct tgtccttcta aaattagtaa tggttccatc
    14521 ttaaatggaa ttactataat agcatataaa aaataaagct aaaaagcaag ctataaactt
    14581 atctaaaaca caacatattg tgttgaaata aaaaacagaa acctaaatat tgtgtcaatc
    14641 tggattctta tggttagagc tctagcgttt tggtgataga agctaagcag ttttaataaa
    14701 gcattaaagt ctgaaaaata aattgtgaaa aacgtgaaaa taaaagaaaa taatttcaca
    14761 ttttttgtcg tttaaatatt taatcttgaa aataaaaaag cattgtgcta taatcggttg
    14821 ttgtaagggt tatcatgtga tggctcaaaa atacacaact taaaaggaga accatactat
    14881 ggcttcaaaa gactttcaca ttgttgcaga aacaggtatt catgcgcgtc cagcgacttt
    14941 gcttgttcaa acagctagca aatttgcttc agacatcact cttgactaca aaggtaaagc
    15001 agtaaatcta aaatcaatca tgggtgttat gagtcttggt gttggtcaag gtgcagatgt
    15061 tactatctca gctgaaggtg cagatgctga agatgccatt gcagcaattg aagaaacaat
    15121 gactaaagag ggattggcat aagactatga cagaaatgct taaaggaatt gcagcctcag
    15181 acggcgttgc tgttgctaaa gcatatctac tagttcagcc ggatttgtca tttgagactg
    15241 ttacagtcgc agatacaaat gcagaagaag ctcgccttga tgttgcactc caagctgcac
    15301 aagacgagct ttctgttatc cgtgaaaatg cagtagaaag cttaggtgaa gaagcagcag
    15361 ccgtttttga tgcccatttg atggttcttg ctgatccaga aatgatcagc caggttaaag
    15421 aaacgattcg cgcaaaacaa acgaatgcag aaacaggtct taaagaagtg actgacatgt
    15481 tcatcaccat ctttgaaggc atggaagata acccatacat gcaagaacgc gcagcggaca
    15541 tccgcgacgt tgcaaaacgt gtgttggctc accttttagg tgtaaaactt ccaaatccag
    15601 ctacaatcaa tgaagaatca atcgttatcg cacacgattt gacaccttca gatactgctc
    15661 aacttaacaa acaatttgta aaagcatttg ttacaaatat cggtggtcgt acaagtcact
    15721 cagctatcat ggcacgtaca cttgagatcg ctgcggtact tggaacaaat gatattacaa
    15781 aacgtgttaa agatggtgat gtgattgccg ttaatggtat cactggtgaa gtgattatcg
    15841 atccaagcga agatcaagta cttgctttta aagaagctgg tgcggcttat gccaaacaaa
    15901 aagcagagtg gtctctcctt aaagatgcgc atactgaaac agctgatggc aaacactttg
    15961 aattggctgc taatatcggt acacctaaag acgttgaagg tgttaatgac aatggcgctg
    16021 aagctgttgg cctttaccgt actgagttct tgtacatgga ttctcaagac ttcccaactg
    16081 aagacgaaca atacgaagct tacaaggcag tgcttgaagg catgaatggc aaacctgttg
    16141 tggttcgtac gatggatatt ggtggcgaca aggaacttcc ttactttgac cttccaaaag
    16201 aaatgaatcc attccttggt ttccgtgctc ttcgtatttc catctctgaa actggggatg
    16261 ccatgttccg cacacaaatg cgtgcgcttc ttcgtgcctc tgttcacgga caacttcgta
    16321 ttatgttccc aatggttgcc cttcttaaag aattccgtgc tgcaaaagca gtctttgatg
    16381 aagaaaaagc aaacttgctt gcagaaggcg ttgcggttgc tgatgacatc caagttggta
    16441 tcatgattga gattcctgca gctgctatgc ttgcagacca atttgctaag gaagttgatt
    16501 tcttctcaat tggaacaaac gaccttatcc aatacactat ggcagcagac cgtatgaacg
    16561 aacaagtatc atacctttac caaccataca acccatcaat attacgtttg atcaacaatg
    16621 tgatcaaagc agcgcacgct gaaggtaaat gggcaggtat gtgtggtgag atggcaggtg
    16681 accaacaagc tgttccactt cttgtcggaa tgggcttgga tgagttctct atgtcagcaa
    16741 cttcagtact tcgtacgcgt agtttaatga agaaacttga ctctgctaag atggaagaat
    16801 atgcaaatcg tgcgcttaca gaatgttcaa cagcagaaga agttcttgaa ctttctaaag
    16861 aatacgtttc tgaagattaa cgggttaaag agttggttta aaccagctct tttttgctag
    16921 ctaaaaatac aattactttt tggtgtgaaa tcatttgagt catcaaatga ttatcagaat
    16981 tttcagatat ttagtgtagc caaatcttga attatatcgg aaaagtgata gaataagatt
    17041 actatcataa aaggagataa aacgttggca aaacaatata aaaatttagt gaacggtgaa
    17101 tggaaactat cagaaaacga gattaccatt tacgcaccag caacaggtga agagttagga
    17161 tcagttccag cgatgacgca ggcagaggta gatgctgttt acgcttcagc taaaaaggct
    17221 ctatcagatt ggcgcgcctt gtcttatgtg gaacgtgcag cttaccttca taaagcggct
    17281 gatattttag tacgtgatgc tgaaaagatc ggcgcgattc tttcaaaaga agtagccaaa
    17341 ggtcacaagg cagctgtcag tgaagttatt cgtaccgctg aaatcattaa ttatgcagca
    17401 gaagaagggc ttcgtatgga aggtgaagtt cttgaaggtg gtagcttcga agccgcaagt
    17461 aagaagaaga ttgctattgt tcgtcgtgaa ccagttggtt tagttcttgc catctcacct
    17521 tttaattatc ccgttaactt ggcaggttct aaaattgctc cagcccttat ttcaggaaat
    17581 gttgttgctc ttaaaccacc aacacaaggc tctatttctg gtttgttact agcagaagct
    17641 tttgcagaag ctggtattcc agcaggtgtc tttaatacca ttacagggcg aggttctgtt
    17701 attggggatt acatcgttga gcacgaagcg gttaacttta tcaactttac aggttctact
    17761 ccaattgggg aaggaatcgg taaattagcg ggtatgcgac caattatgct tgagcttggc
    17821 ggtaaggatt ctgctatcgt tttggaagat gcagatcttg ttttagcagc gaaaaatatt
    17881 gtagccggtg cttttggtta ctcaggacaa cgttgtacag cggttaaacg cgttcttgtg
    17941 atggacaagg tggcggatca attggcggct gagattaaaa cacttgttga aaaactaagt
    18001 gtcggaatgc ctgaagacga tgctgatatt acaccattaa ttgatacatc agctgctgat
    18061 tttgttgaag ggttgattaa agatgcaact gataagggag ctactgcttt gacagccttt
    18121 aatcgtgaag gcaatcttat ttcacccgtt ctctttgatc atgtgacaac tgacatgcgt
    18181 ttggcatggg aagagccgtt cggcccagta ttaccaatta ttcgtgtaac cactgtagaa
    18241 gaagccatca agatttctaa tgagtctgaa tatggtttgc aagcttctat ttttacaact
    18301 aatttcccaa aagcttttgg cattgctgag caattagaag ttggaactgt tcaccttaac
    18361 aataaaacac aacgtggaac agataatttc ccattcttag gcgctaaaaa atcaggtgca
    18421 ggggtacaag gagttaaata ttctatcgaa gctatgacaa ctgttaaatc tgttgtattt
    18481 gatatccagt aataaaaaaa ataaacaaaa agttaggtta acttaacttt ttgtttattt
    18541 ttttaatgtt ttttgttaca atagtctata cagatggagg caactattga aaaaattaaa
    18601 tgttattctt gttggtttat taagcattct gattttgagt ttagctattg tgtttattaa
    18661 tcgttggaaa ctaaacgaag atagtcagcg tatagttttg gctgaaaaga aaaaaaacac
    18721 gtcagattta gtgatcaaag ctgtaaaaca tattaaaaaa gatcaaaaag actattatta
    18781 tttttccccg ataaaacaag cagatgattt ttttgtagat aatttacctg tttcattata
    18841 caaaaaaaag aattcagata aagaattgat tttagtaaag cctaaactgc aatcttctca
    18901 cctaagatca gttaacactt tgactatttc taaaatagtt tatcagaaaa aattttttca
    18961 tttggctaaa aaatcagaaa aagttataag tacatatcac gttacagacg acttgaaacc
    19021 gtttcaggta agggatctag tatcaggaca tttagaaaga atacaagaag aagttgaaaa
    19081 aaaatatcca aatgctggtt ttaatagcga taagtacaat ggcttaaaag aatctaattc
    19141 tttattaagc gatggctttg aggtaaaatc gggaaacctt atttttgata aaaagctaac
    19201 gatacctttg acgacattat ttgatgttat taatccagat tttttagcaa atagcgatag
    19261 agctgcgtat gataattata ggacctacaa agaacagcat cccaaaaaac tagttgcatt
    19321 aacgtttgat gatggtccag atccgacgac gactcctcaa gttttagata ttttggcaaa
    19381 ataccaggct aagggaactt tctttatgat aggttcaaaa gttgtgaata atgaaaacct
    19441 tactaaacgt gttagcgacg ctggccatga aattgctaat catacttggg atcatcccaa
    19501 tctgactaat ctttcagtca gcgagattca acatcaagtt aatatgacga accaggctat
    19561 tgaaaaagct tgtggcaaga aacctcgcta tttacgccct ccatacggag ctacaaatgc
    19621 aactgttcag caatcctcag gattaacaca aatgctttgg acggttgata caagagattg
    19681 ggaaaatcat agcactgatg gcattatgac taatgtcaaa aaccaattgc aaccgggagg
    19741 agttgtttta atgcatgata ttcatcaaac aactatcaat gctttgccga cagttatgga
    19801 atatctaaaa gcagaaggat acgaatgtgt gactgtatca gaactctatg cgcatcagta
    19861 agtaatatcg ttatgtttct ttagaattga tatactaaac aatccaatta ctttgaagta
    19921 attggattgt tgtttttagt agcccttgat taagttttgt tttttaacaa ctagttgatg
    19981 aaatgaagtt gttgattttt gagggagatt tcagagactt tagcaaattt tttcaatttt
    20041 ttgaggtctt cagggtggct aacaaaggta ataacgatac cagattttcc catacggccg
    20101 gttcgtccag ctctgtgagt ataattttcc ttatcacgag caacatcaaa atgaatgaca
    20161 tagtcaagat tatcaatatc aatgccccga gcaacaagat ccgtagctaa caataaggag
    20221 agttggtggg atttgaattt ttcgagaatg gttttacgga atttgacatt gatgtcactc
    20281 gctaaggaaa ccgcagcagc tccattatat tggagacgtt cttccgtagc acccaaatca
    20341 gataaactgt tgaaaaagac taaagctcta aaatgaggaa tattagcaaa ttttcgtaat
    20401 aaatcggtac gttcgcgctt gtcaaccatg aggtaaaaat gctgaatagc atcgttggtt
    20461 tgctctgaaa gatcgatgca gaatgtattt ggagctaagg aagtttgatc caccttatta
    20521 gtagcactca tgtataccat ttgatgatct cgaggaacat agtggctaat tttttgaaca
    20581 aaatcatatt gggaatcacc taatagttcg tcatattcat ctaaaacaat ggtgttgaca
    20641 gacatcatct tgattttttt caatttaatc agttcaaaaa tgcgtccagg ggtcccaatc
    20701 aggatttcag gacctttttt gaggcgttcg atttgacgtt tttgactggt tccagaaata
    20761 aagagttgtg ccgttaagcc tagtggctgt gcccaatcct ttgtgacttc aaaaatttgg
    20821 ccagctaatt cggtattagg ggctaaaatg agtagttgtt gggacttttt aggcgtcagt
    20881 gccaatagac taggaaagac ataagctaag gtcttgcccg tcccagtagg gctaatacca
    20941 aggaaatttt taccatcaac aataggttga aaggcctgtt cttggattgg cgttaaatgg
    21001 gtaaatgcaa cttggtcaag tttttcttgc cattgagggg gaaatttagt gatcatgcgt
    21061 cttcctttta ctattgtttc tatattcatt atagcattat agtctcaatt ttggtaaaat
    21121 agtgagaaag actttttaga tttggagttt aaaatgctta aaaaaccgat tattattggt
    21181 gtaactggtg gttcaggtgg cggtaaaaca agtgtgtcac gcgctatttt ggacagtttt
    21241 ccaaatgctc gtattgcgat gattcaacac gattcttact ataaagatca gtctcacatg
    21301 agttttgaag aaagggtcaa aaccaattat gaccatcctc tagcttttga tacagatttt
    21361 atgattcagc aactaaaaga attattagct ggtagacctg tagatattcc tatctatgat
    21421 tacaaaaagc atacccgcag taatacgacc tttcgtcagg atcctcagga tgttattatt
    21481 gttgaaggta ttcttgtcct agaagatgag cgtttgcgag atttgatgga tatcaagctc
    21541 tttgtagaca cagatgacga tattcgtatt attcgtcgta ttaagcgtga catgatggaa
    21601 cgtggcagaa gcttagaaag tattattgat cagtacacaa gcgtggtaaa gcctatgtat
    21661 catcagttta ttgagccaag taaacgctat gctgatattg ttattccaga gggagttagt
    21721 aatgtcgttg ctattgatgt tattaatagt aaaattgcga gtattcttgg agaagtatag
    21781 tggaagtggc tgagtagatt atttaaatct gctcttttat tgttgtgctc ttcatagaga
    21841 aagggatcat aaaatagaaa tgttattaat ttggcttgga ttactatttg tttggaatga
    21901 actcgtgttt gtcatatatg cctttgataa aagaaaagcc ataaaaaaga aacggcgtat
    21961 tagtgaaaga aaattattag tgataacagt tttgtttgga ggttttggag cattacttgc
    22021 agctaaaaag taccaccata aaaccagaaa gtggtacttt gtcatcactt ggtatactag
    22081 tattttgttg acattactag taacttatat ctttatgaag tgagtcactt aaaagaccct
    22141 taatcagtat tatttagtca aggctctttt atagtttaaa aaagcacatc atcgtcaagg
    22201 gtgttgattt tttgaaaaag atcaccaaag attaaaagga ttaatgttcc tagtaaaact
    22261 gtcatgccag gaatgcttgt tagaggagag ttccagatga aatgtagaac cagaggactt
    22321 aataaccaga aaatttgttg ctgttttgaa aaaaggttag aaccctttaa taataagtat
    22381 gctccaatgg taaaaatagc tgtatgtacc caatgtgaag tcacagcagt gctaatacgt
    22441 tcaatagcag tcggtacgag actatctata gattttgctt gaatcaaata acggatatct
    22501 tctgttagtt gaaagcccat gccagaaagt aaagcaacaa ctaatttttc tttaagagta
    22561 agtctaggaa ataaagtaat tactaaaata gccgaagaag ccttaatggg ctcttcgaca
    22621 ataggggcag ttaaagcatc tccccaaagc tttaaagcgt ctttagaagg gatgatgtat
    22681 gaccaaaatt gtcctacgag atggttccca caagaagcta aaaaacctga aatataaagt
    22741 ccgctaagac agctaacaat aataacgttc aggttcatat gaaatttttt acttaaataa
    22801 aaaatagctg ctgcaaatgg aattaagtaa atgcctaata gactaatggt tagtattaga
    22861 gcaaaagctt ctttaaagga aaggttgtta gctgtcagtc ctaacaattc aagttcaata
    22921 ccatttaaaa ctaaagcaat aaaaagaccg tatcgtaacc atttctgttg agtgatttgt
    22981 ttcacgaaaa cctcctaata ttaagatgat attaataata ttgaaaatta tatcataaac
    23041 aacctcaaaa accattttaa aaactgactg aagcgaaaga tagtcgtaaa aaatactcga
    23101 atagacccag atatgctata ataaaaggta tgaataagtc taaaaaaatt gagcaatacc
    23161 aactaatgat agcgcaggca aaagagctgt ttgcgaatga atcaaatgct ttggctaatt
    23221 tgtcaaatgc cagcgccctg ttaaacatga cactacccaa ttctgttttt gcgggatttt
    23281 acctttttga tgggcaggaa ttaattcttg gcccttttca aggtagggtt tcttgcgtcc
    23341 atatcaagct tggtaaaggt gtttgtggcg aatcagctca gtctcgtcga accattatta
    23401 taaatgatgt gaagcagcat gccaactata tttcctgtga tgcggcagcc atgagtgaaa
    23461 ttgttgtgcc tatggtgaaa gaaagccatt tgattggggt gctggacttg gattctagcc
    23521 ttgtagctga ttatgatgag gttgatcaag aatatttaga ggcctttgtg gacctacttc
    23581 ttgaaaagac cacgtttacc tttaacatgt ttggagttaa aaactgatgt atcaagctct
    23641 ttatcggaaa taccggagcc aaacgtttga cgaaatggtg ggacaatcgg ttatttccac
    23701 aactttaaag caggcagttg aatctggcaa gattagccat gcttatttat tttcaggtcc
    23761 tagagggact gggaaaacaa gtgcggcaaa gatttttgca aaggccatga attgtcctaa
    23821 ccaagtcgat ggtgaaccct gtaatcaatg cgatatttgc cgagatatca cgaatggaag
    23881 cttggaagat gtgattgaaa ttgatgctgc ctcgaataat ggtgttgatg aaattcgtga
    23941 cattcgagac aaatcaacct atgcgccaag tcgtgcgact tacaaggttt atattattga
    24001 tgaggttcac atgttatcaa caggggcttt taatgcgctt ttgaaaactt tggaagaacc
    24061 gacagaaaat gttgttttta tcttggcaac aacggaattg cataaaattc cagccactat
    24121 tttatctcgt gtgcaacgct ttgaattcaa agctattaag caaaaagcta ttcgagagca
    24181 tttagcctgg gttttggaca aagaaggtat tgcctatgag gtggatgctt taaatctcat
    24241 tgcaaggcga gcagaaggag gcatgcgtga tgctttatct attttagatc aggctttgag
    24301 cttgtcacca gataatcagg tcaccattgc aattgccgaa gaaattacag gttctatttc
    24361 catgcttgct ctgggtgact atgttcgata tgtctcccaa gaacaggcta cgcaagctct
    24421 ggcagcctta gagaccattt atgatagtgg gaagagcatg agccgctttg cgacagattt
    24481 attgacctat ctgcgtgatt tattggtggt taaagctggc ggcgacaatc aacgtcagtc
    24541 agctgttttt gataccaatt tgtctctctc gatagatcgt atattccaaa tgataacagt
    24601 tgttactagt catctccctg aaatcaaaaa gggaacccat cctcggattt atgccgaaat
    24661 gatgactatc caattagctc agaaagagca gattttgtcc caagcaaact tgtcaggaga
    24721 gttaatctca gagattgaaa cgctcaaaaa tgagttggca caacttaaac aacaattgtc
    24781 gcagctccaa tcgcgtcctg attcactggc aagatctgat aaaacgaaac ctaaaaccac
    24841 aagctacagg gttgatcggg ttaccatttt gaaaatcatg gaagaaacgg ttcgaaatag
    24901 ccaacaatct cgacaatatc tagatgctct aaaaaatgct tggaatgaaa ttctagataa
    24961 catttctgcc caagacagag ccttattgat gggctctgag cctgtcttag caaatagtga
    25021 gaatgcgatt ttggctttcg aggctgcctt taatgcagaa caagtcatga gccgaaataa
    25081 tcttaatgat atgtttggta acattatgag taaagctgct ggtttttctc ccaatattct
    25141 ggcagtacca aggacagatt ttcagcatat tcgtaaggaa tttgctcagc aaatgaaatc
    25201 gcaaaaagac agtgttcaag aagaacaaga agtagcgctt gatattccag aagggtttga
    25261 ttttttgctc gataaaataa atactattga cgactaaaac tgaaatacat aagggtaagg
    25321 ttttgccctt acccacgttt tatctcgtta ctttttttca tagtacagta tttttttgtt
    25381 ataataaagc tatgactatt cgacaatttc tttttatggc ctttgtatgt gcctttgaaa
    25441 cctatttttt taatgacctg ttgctctctg gaaattatct cttcgcctta ttttggggct
    25501 tgttgctctt tagggattta agaagagtgc ataccattaa tcaattgact aaaaccattt
    25561 taaaagcggc taattctccc aagaagaaag actgatttca ccgcttcgca aggtttgaag
    25621 gtcaccattg tcaaattgga caataaggtg tccttgatct gtgagatcaa tcgcaatagc
    25681 acgtttttct tgactatttt ctataaaggt cacttgttta ttaagaacaa gtgatttttc
    25741 tttgtaaact ttgacatggt ccttgacagg aatagataag aataatttcc agatgtcaat
    25801 gataaggtca ttgcgcgtga tagtcggttt ttcggtaaag agagaacctg ccttttgagc
    25861 aatcgcttct gggaagtcgg tgacaaaaaa gttgagacca accccaataa tgacatcggt
    25921 gattaaacca gtttcaacag aagtaatggc ttctgtcaga atccctgcaa ctttatggtt
    25981 acctagatag atgtcgttta cccatttgat ttctgtatca atgcctgtta atcgcgagat
    26041 agctttgaca atactggaag caaccatcat cgtataagga ggcatatcag cataagggac
    26101 attgggtttt aggtacatgg acatgtaaat accaccggtg ctagctgaaa aaaattgccg
    26161 atcgaggcgt cctttggcag cttcttggct aggagctagg taaagccttg gagcgctgtt
    26221 atgagcttcg attccttgtt tggcatctaa ttgggtagat tggctgtgag gggtataggt
    26281 gactggcatc ccaagccctt ggctaatggt tttgggtaat aaaatatcac cttgtaccat
    26341 gcggtagccc ttgtgtttta aagaatcaat ttgaatcccc tgattttcga gacttttgat
    26401 ggatttccaa acggatgtac gagagatact gagttgatct gctagatatt ccccactgac
    26461 gaagtcatcg gtttgtgaca gtaatt
//
LOCUS       DAUGFP010000022        25299 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.022, whole genome shotgun sequence.
ACCESSION   DAUGFP010000022 DAUGFP010000000
VERSION     DAUGFP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25299
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.022"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            178..426
                     /locus_tag="VH663_000938"
     CDS             178..426
                     /locus_tag="VH663_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ8174149.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            463..1074
                     /gene="amaP"
                     /locus_tag="VH663_000939"
     CDS             463..1074
                     /gene="amaP"
                     /locus_tag="VH663_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEQ8174150.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVQLRHKNK
                     KGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRKFKIIVKGRLASRVAVADQIS
                     GVKEGIEKGLTEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            1085..1273
                     /locus_tag="VH663_000940"
     CDS             1085..1273
                     /locus_tag="VH663_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEQ8174151.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            1286..1825
                     /locus_tag="VH663_000941"
     CDS             1286..1825
                     /locus_tag="VH663_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ8174152.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1866..2066
                     /locus_tag="VH663_000942"
     CDS             1866..2066
                     /locus_tag="VH663_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ8174153.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            2077..2565
                     /locus_tag="VH663_000943"
     CDS             2077..2565
                     /locus_tag="VH663_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ8174154.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTNAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            2728..3369
                     /locus_tag="VH663_000944"
     CDS             2728..3369
                     /locus_tag="VH663_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8174155.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(3512..4054)
                     /locus_tag="VH663_000945"
     CDS             complement(3512..4054)
                     /locus_tag="VH663_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ8174156.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKTVTLDN"
     gene            4172..4873
                     /locus_tag="VH663_000946"
     CDS             4172..4873
                     /locus_tag="VH663_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174157.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEIDKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHCTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4888..7524
                     /locus_tag="VH663_000947"
     CDS             4888..7524
                     /locus_tag="VH663_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ8174158.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMGLGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWPKRETDLVLSSSLAKNYQIGDELAVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRAALAKETVKLAASEENL
                     TFLEVSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSTTSLSPYFNLLDC
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNVNHIKSIQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            7616..8212
                     /locus_tag="VH663_000948"
     CDS             7616..8212
                     /locus_tag="VH663_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ8174159.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLVNL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDSPIGQALLDAADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            8223..9179
                     /locus_tag="VH663_000949"
     CDS             8223..9179
                     /locus_tag="VH663_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ8174160.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGPQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVSTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            9276..10616
                     /locus_tag="VH663_000950"
     CDS             9276..10616
                     /locus_tag="VH663_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEQ8174161.1"
                     /translation="MNEIKCPHCHTLFTINKSEYSQLLEQVRGQAFDEELQKRLINEI
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANEQVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFAKDNQLSSRGSKGDYIYREVDANGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALVKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVKRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFEALKDQ"
     gene            10732..11616
                     /gene="truB"
                     /locus_tag="VH663_000951"
     CDS             10732..11616
                     /gene="truB"
                     /locus_tag="VH663_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEQ8174162.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISQFERTSPLNFT
                     EDDLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            11645..12577
                     /locus_tag="VH663_000952"
     CDS             11645..12577
                     /locus_tag="VH663_000952"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEQ8174163.1"
                     /translation="MEIEYIKDYRDINQEDDTVLVLGYFDGLHRGHKALFDKAREVAN
                     KEGLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNRTDSDYLTRNFEGQVYTIEEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            12620..13024
                     /locus_tag="VH663_000953"
     CDS             12620..13024
                     /locus_tag="VH663_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEQ8174164.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDIDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            13026..13304
                     /locus_tag="VH663_000954"
     CDS             13026..13304
                     /locus_tag="VH663_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEQ8174165.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLDS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSIYQVVKKAKAIEKGFFSLGN"
     gene            13294..14082
                     /locus_tag="VH663_000955"
     CDS             13294..14082
                     /locus_tag="VH663_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEQ8174166.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVILEDQ"
     gene            14085..15395
                     /locus_tag="VH663_000956"
     CDS             14085..15395
                     /locus_tag="VH663_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEQ8174167.1"
                     /translation="MMSLPKEFINIYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKESRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALTMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLTKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKSDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
     gene            15534..16400
                     /locus_tag="VH663_000957"
     CDS             15534..16400
                     /locus_tag="VH663_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEQ8174168.1"
                     /translation="MKMKKKFFLLSLLALSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            16411..17346
                     /gene="pstC"
                     /locus_tag="VH663_000958"
     CDS             16411..17346
                     /gene="pstC"
                     /locus_tag="VH663_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEQ8174169.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTILSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATRWQTIWRVVLNAARP
                     GIFTAVIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            17336..18223
                     /gene="pstA"
                     /locus_tag="VH663_000959"
     CDS             17336..18223
                     /gene="pstA"
                     /locus_tag="VH663_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEQ8174170.1"
                     /translation="MNAKKVDKVATGTLYIIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSTMTAAK"
     gene            18239..19042
                     /gene="pstB"
                     /locus_tag="VH663_000960"
     CDS             18239..19042
                     /gene="pstB"
                     /locus_tag="VH663_000960"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174171.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            19055..19813
                     /gene="pstB"
                     /locus_tag="VH663_000961"
     CDS             19055..19813
                     /gene="pstB"
                     /locus_tag="VH663_000961"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174172.1"
                     /translation="MTEPILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            19881..20534
                     /gene="phoU"
                     /locus_tag="VH663_000962"
     CDS             19881..20534
                     /gene="phoU"
                     /locus_tag="VH663_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEQ8174173.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
     gene            20739..23276
                     /locus_tag="VH663_000963"
     CDS             20739..23276
                     /locus_tag="VH663_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEQ8174174.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQPTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
     gene            23622..24296
                     /locus_tag="VH663_000964"
     CDS             23622..24296
                     /locus_tag="VH663_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8174175.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRSVGY
                     ILKNNE"
     gene            24289..>25299
                     /locus_tag="VH663_000965"
     CDS             24289..>25299
                     /locus_tag="VH663_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ8174176.1"
                     /translation="MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYS
                     SVDSSLVSVSNNASSYANRTMARISSFYFDTENNIIKALPDSDSSKLLGTPAANTDII
                     LFSANGTILNAFDAFSNYQNFHLDKRRLGSIETTSLMNFYGQEEKYHTITVRVHIKNY
                     PAVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEK
                     QKMFVENASHELRTPLAVLQNRLESLFRKPNETILENSEHLASSLDEVRNMRILTTNL
                     LNLARRDDGINPQWTHLDTDFFNAIFENYELVAKEYGKIFYFQNQVNRSLRMDKALLK
                     QLI"
BASE COUNT         8040 a         4169 c         5197 g         7893 t
ORIGIN      
        1 cattttatag gattttgaaa tatcggtatt caaacaaaaa ataaactaac actttattat
       61 ttagtttata tagtcacttt ttgaaagtcg actattaaaa gactattgct tgaagaataa
      121 cagctgttat tcttccttgg acaaaaggta caggagtatc taaaaaggag aattatcatg
      181 ggacttattt ggactttaat cgtcggtgcg cttattggtg tgattgctgg agcacttact
      241 aaaaaaggtg gttcaatggg ctggattgca aacatcgctg ctggtctagt tggttcttcc
      301 gttggtcaag ctttacttgg ttcatggggc ccttctttag caggaatgtc tttgatcccg
      361 tcagttattg gtgccgttat cgttgttatg atcacttctt ttgtactaaa caaaactaac
      421 aactagtttc ctctttattt agtaagtact tacaaataga gaatgttaag tagaaaagag
      481 gtctgtatgt caaaattgtt aaagattagt tattgtcttg tcggacttgt tctattgtct
      541 gtttttggat gggtagttgg aataaccgga ggctatatat atttacctta tagttatcgt
      601 tggctaagtt ggggtatgga tagttttccc aatctcttag attcagcttt atcctattat
      661 tacttctgga cagcccttgt attatttgtt ataacatttc ttgccttatt agttattatt
      721 ttatacccaa gaatttatac tgaggtccag ttacgccata aaaataaaaa aggaacactt
      781 ttattaaaaa aatcggctat tgaaagttat gttgcgaccg ctattcaaac tgcaggtttg
      841 atgccaaacc caacagtcac agctaaatta tataagcgga agtttaagat tatcgttaaa
      901 ggacgtttag cttctcgtgt agcagtggca gatcaaatta gtggcgtgaa agaagggatc
      961 gaaaaaggtt taactgagtt cttcggaatt aattatcctg ttaatttcaa agtttatgtt
     1021 aaagatattg ctgattctga tcgtaaacat attaccagaa atcgtgtaga ataggagatc
     1081 tatcatggaa ttttatgaaa aattcaagta ccctattatt ggtggcttgg tggggctaat
     1141 tattgccata ttgctcatgg cttttggttt attcaaaacg ctactagcaa ttattttcat
     1201 tattttagga atatatggtg gcctgtatgc caaaaaaaca ggtattattg atcaatttct
     1261 taatcgcaaa taaaggagac ttattatgac tgaaacttat attaaaaaca catctaaaga
     1321 cttaacttca gcaattcgtg gtcaattaac atatgatgat aaagtaattg aaaaaattgt
     1381 tggtttagca cttgaaaatg tcgatgggtt actgggtgta aatggtggtt tttttgctaa
     1441 cctaaaagat aaattagtaa acaccgaatc tgtacgtgat ggcgtgaacg tggaagttgg
     1501 aaaaaaacaa gttgccgttg atcttgatat tgtcgctgaa tatcaaaagc atgtgccgac
     1561 tatttatgac tctattaaat cgatcgttga agaagaagtg aagagaatga ccgatctgga
     1621 tgtgattgaa gttaatgtta aagtagtcga cattaaaaca aaagaacaat ttgaagctga
     1681 aaaagtaagt ttacaagaca aagtttcaga catggcacgt tcaacatctg aatttacgag
     1741 tcatcaagtt gagaatgtta aagcttcagt ggataacggt gttgaaaaac ttcaagatca
     1801 aaaagctgag ccacgtgtaa aataattata tgtcgtcaat taggtaagct aaaggagggt
     1861 ttattgtgtc agatgaaaaa tataatgcga aattagatca agctggtggc aagttaaaag
     1921 aaggatttgg taagatttct ggagataaat ctttagaaac tgaaggtaaa gtagataaag
     1981 ttactggaaa agttaaagag gttattgcag acgccaaaga tactgtaaaa ggattagcta
     2041 aaggcttaga taataaagat aaataaaggg gacatgatgg aaaaaactca aattaaaagc
     2101 actcttactt atgacgataa ggtcatcgaa aaaattgtcg gtcatgcttt ggaaaacgtt
     2161 ggcggattac tagctgtaac aggtggattt ttctcaaata ttaaaaataa tttggtgaat
     2221 tctgagtctg taactgatgg tgtttcagtt gaagttggta gcaaggaagt agcagttgat
     2281 ttagcaatca ttgtagaata cggtaaagat attcctgcta ttgcagaatc tattaaagct
     2341 attgtttctc aaaacgttga tagtatgaca catttgaaag ttgttgaagt caatgtgaac
     2401 gttgttgata ttcgtactaa ggaagaacat gaagctgcta gcgttactgt tcaagatcgt
     2461 gtcactaatg cagcttcaag tacttcacaa tttgtatcag aacaaacgga aaaattaaaa
     2521 gatactatct ctgatactgt aaatagtgat gaggcagcta aataagcaat cgtctaggaa
     2581 gtattattga ctagtggtga taagtttgag ctggtattta ccagctcttt ttgtcacggt
     2641 taaaaagaca cggactattg tcatctctct aaaaatatga tttaattaga tagaaatttt
     2701 tcaaattgtt agaaaggagt aaattaagtg ataagtccta aaaaagagat aacaagttca
     2761 aaatatcaaa aaattgctat ttcagtagct cagcgtatcg caaatggcga atatgaagtg
     2821 ggcgaaaagt taaagtcaag aactactatc gcctcgacat ttaatgtatc accagaaacg
     2881 gctcgtaaag gcctgaatat tttagctgac ttgaaaattt tgactttaaa acatggtagt
     2941 ggagctattg tgctgtcaaa agaaagagct attgagttta taaatcaata cgaatcaacc
     3001 cattccatcg ctgttttaaa agaaaaaata cgtgagacta ttaatgatca gggaaaagca
     3061 atggaaaaaa tggctgtttt ggtcaatgat tttttaatgc aaagtcaatc tgttagtaag
     3121 caatacccat tagcacctta tgagattatt tgtagtcaag attcggaaca ttttggcaaa
     3181 tctattggtg tgttaaatat ctggcaccag acaggagcaa ctatcgtcgc tattgaacat
     3241 gctggtcagt ttatcgtttc accaggtcct tattctgtta ttgaaaaagg cgatcatatt
     3301 tatttcgttg gtgatgagtc agtcatttca cgaatgaaga ccttttttaa tttacgaaaa
     3361 ggattataag tttgagggcc cacctcaaac tttttttgtt gctgttgagc attattaaaa
     3421 gaggcagatc atcatggttt gtctttccaa aatatctgtt tagacagttg ttttggattg
     3481 ggagcatgat ttcagactct tttgatgttt tttaattgtc tagagtaact gttttgagaa
     3541 taatatcggc aacttcttca gtggtttctt gatagccatt agcaatccaa tagctgataa
     3601 tgctctcaat actggcaagg taaacttcta aagcataggg ataaggaata tggtattgat
     3661 tggtcacaat gtcttggaaa ccagtaatag tagtcaaaaa ttggtaacag aaatccttga
     3721 tgagttgcgg aaatttgagg taagaaatag tagcgagtgc ggttataaat tctctatgtt
     3781 ctataaggta ggacaaggtt tgattgagga cttgtcgagt gtctgtgtag atttcagcct
     3841 ggtttaacaa acggtacaaa tcatccagag tgtcattttt gaagtgattc atcatgtcaa
     3901 atttatcagt gtagtggagg tagaaggttc ctctattgat accagccttt ttagtaaggt
     3961 cagagacagt aagagtttca aaagattgtt cagttagtaa ggtggttaag gcggtcttta
     4021 cataagcttt ggtttcagta tgtcttttgg tcattctcag gtgctcctta aatcaacagt
     4081 tgcgtcagat ttgttcttgt taaatactta gggccattat ataataaggc tataattaaa
     4141 tcaacactat gttcaaataa tggaggggca gatggctttt attgaattaa aacaggtttc
     4201 aaaatcttat caggtaggag agacgactgt ttttgccaac cacgaagtgt cttttgagat
     4261 tgataaagga gagctggtgg ttattttggg agcatccgga gctggtaaat cgacagtcct
     4321 caatattttg gggggcatgg acacagtaga tgcaggtcaa gtaattattg atggcaaaga
     4381 cattgctcat tgcacatcta aggccttaac tcagtatcgt cggaatgcta ttggtttcgt
     4441 ttttcaattt tataatttgg ttcctaattt gacagctaaa gaaaatgttg aattagcagt
     4501 tgaaattgta gcagatgctt tagatcctgt gaccatttta aaggaagtag gactcagtca
     4561 tcgtctggat cattttcctg ctcagctctc aggtggtgaa cagcaacggg tttcgatagc
     4621 acgtgcctta gctaaaaacc ctaaattgct tctttgtgat gaacctacag gtgcccttga
     4681 ctaccaaaca ggaaagcaaa ttctaaccct cttacaggat atggcacgaa ctaaggggac
     4741 cacggtagtt attgtaaccc acaatgctgc tattgctccg atagctgatc gtgtcatttt
     4801 tatgcatgat gcacaggtta ccaagacagt cattaataag gaacctgcaa gcattgagac
     4861 aattgattat taagaggaag gtgtcgtatg ataaagaaaa ccttatggaa ggatattttg
     4921 agagctatca agaatagtaa agggcgattc atttccttat ttttcctaat gggcttaggc
     4981 tcctttgctt tagttggact taaagtcacc ggtccagata tggaacgaac agccagccgt
     5041 tacctcgaaa gacaccaggt gatggaccta acggtactgg cttctcatca attttcccaa
     5101 gccgataaac aagagttaga tacgttaaaa ggggctcatt tagaatatgg tcatttactt
     5161 gatgtcagtc taactagcaa ccagaaatct cttaggctct atagcgtgcc aaagaaagtg
     5221 tctaagccag tcttggttaa agggagttgg ccaaagcgag agacagattt ggttttatcc
     5281 tcctcacttg ctaaaaacta tcagattgga gatgaactag cagtcacctc acctatggaa
     5341 ggtttgctga cgacaaccca ttttcaagtg gttggttttg cgaattcttc agaagtttgg
     5401 tccaagtcca atttaggaag ttcctcaacc ggagatggaa gtctctacgc ttatgctttt
     5461 gttaatccaa acgtttttaa aagtgccttt aacctgctgc gcatccgttt tagtaatctt
     5521 cgtctgacaa acgctttttc caaagactat caaaaaaggg taacccaaaa ccaagctcat
     5581 ttggataatc tacttaaaga taatggccag aagcgttatg atgacctcca aaatcagtat
     5641 gaccttgcct taaaaaatgg cagagcagca cttgcaaagg aaacagtgaa actagctgcg
     5701 agtgaggaga acttaacttt tttagaagtc tctgctttac aagaagctaa gcatcagatt
     5761 gaacaaggca aacaagcatt agccaaggag gaaaagcagt tagagcaggt gcaggctaca
     5821 aaagataagc tagaaaaacc cagttacctg acttataatc gctcgaccct accaggagga
     5881 gaaggatatc atacttatgc aacttcaacg acctccattt caaatgttgg aaatattttt
     5941 cctgttgttc tttatctcgt agctgctcta gtagccttta ccaccatgac acgttatgtt
     6001 gatgaagaaa gaacaagctc tggtctatta aaagccattg gttattctaa caaggatatc
     6061 agtttaaagt ttcttatcta tgggctttta gccagttttt tagggacaac tttaggtatt
     6121 attgggggaa cttacctctt atctgccttg atttcagaga tcttaacagg agctttgact
     6181 attggaaaga ctcaccttta tagttattgg ttttataatg gcatagctta cttgctggct
     6241 atgttatctg ctgttttacc agcctactta attgtcaaaa aggaattatt cctcaatgca
     6301 gctcagttat tgctgcccaa acctcctagt aagggggcaa aaatctggtt ggaacacctt
     6361 acttttgtct ggaaagctct gtcctttact cacaaggtga ccatacgtaa tatttttcgc
     6421 tataaacaaa gaatgctgat gaccattgta ggcgttgcag gctctgtagc ccttttattt
     6481 gcaggcttag ggattcagtc gtcattagcc aaagtagttg agcatcaatt tggtgattta
     6541 acgacttatg atattttggc tgtcggttcg gccaaagcga cagcgacaga gcaaactgac
     6601 ttagctagct atcttaaaca agaacctatt acagggtacc aaaaggtatc ttatgccagc
     6661 ttaacccttc ctgtaaaggg attacctgat aagcaaagta tttccatttt atcaagttca
     6721 actacctctc ttagccctta ttttaatctg ctggattgtc aggagcaaaa gaaggttccc
     6781 attccaacct ctggtgtttt gatttctgag aaattagcct cttattacaa ggtaaaacca
     6841 ggtgatcagt tggtattgac tgatcggaaa ggacagtctt ataaagtgac gattaaacag
     6901 gttattgaca tgacagttgg ccattacctg ataatgtcta atacctattt taagaatcat
     6961 tttaaaggat tggaggctgc tcctgcctat ctgattaagg taaaagacaa agatagcaag
     7021 cacataaagg agacagccag tgacttgtta accttaaaag cgattagagc agtttcacaa
     7081 aacgtcaatc atattaaatc tattcagcta gtagtcacct ctcttaatca ggtcatgacc
     7141 ctccttgtct tcttgtctat tttattagca atcgttatcc tttataactt aacgactatt
     7201 aatattgctg agcgtattcg agaattatcc actattaaag ttctgggatt ttacgatcag
     7261 gaggtcacct tatatattta tcgagaaact atttcgctat ccctagtagg cattctttta
     7321 ggtatctatt taggaaaagg cctgcatact tatatcatga caatgatttc aactggggat
     7381 attcaatttg gtgtaaaggt tgatgcttat gtttatctag tgccaattct agtaatcctt
     7441 agcttgttag cggtattagg tatctgggtt aatcgccatt taaaaaaggt tgatatgtta
     7501 gaagctttga aatccataga ttgattggtg aaaagacaga tatagacagc acttgaaaag
     7561 ttaatcatat tatgatagta tctaaatact ataatttaat agaaagatac tatgtatgga
     7621 aaatagcatt gaggcctatc tagcagatag cagagaaaag gtaacattag gtacagttgt
     7681 tgattgtttt aaaggaaaag cagtctcaag taaggttgtg ccaggtgatg tgggattggt
     7741 taatttatct gacatgggca ccttagggat tcaatatcac caattaagaa ctttccagat
     7801 ggatcgtagg cagctcttgc gttacctttt ggaagacgga gatgtcttaa tcgcctctaa
     7861 gggaacccta aaaaaagtct gtgtttttca taagcaaaat cgagatgtgg tggcatcatc
     7921 aaatatcact gtcttacgcc ctcaaaaact gttaaggggc tactatatca agttcttttt
     7981 ggactcacct attggtcaag cgctcttgga cgcagcggac catggcaaag atgtgatcaa
     8041 tctctcaacg aaagaattgt tggatattcc aattcccgtt attcctttag tgaaacagga
     8101 ctatttgatt aatcactact tgagaggatt aacagattat catcgtaaac tcaatcgagc
     8161 agaacaggag tgggaatata ttcagaatga aattcaaaaa ggattaggtt aggagtcttg
     8221 tcatgggttc agttgaacgt acattaaaaa tgaccttggc taccattgtt gccattttaa
     8281 ttgcttacca acttcatctg gattatgcaa tgtcagcggg gatcattgcc ctgttaagtg
     8341 ttttagacac ccgtaagtcc agtttggtgg tcgctaggaa tcgcttgcta tcttttttcc
     8401 tggctttcgg gattgcgatg atgtgcttta gcttgtttgg ttttacgaca gtaggtttta
     8461 tgtgttattt gttaattatt attcctttgc tctatcactt tcagatagag gcaggactgg
     8521 ttcctataac agtacttgtg acacatttga ttgctaaaaa gagtattgca ttacctattt
     8581 tatcgaatga gtttatgctc ttttttgtag ggactagtgt cgctttacta tttaatgctt
     8641 atatgggtcc acaagatcag caaattcggt actatcacca aaaagttgaa tcggatctga
     8701 aaggtatctt ataccgtttt gaaagttttc tactggaagg aaaaggtcaa aatgaagggc
     8761 tcttgatcaa aaaccttgat aaaatattag atgaagctct aaaactagtt tatcgagaaa
     8821 ggcataatca actatttcag cagaccaatt atcaagttca ttattttgag atgcgacgac
     8881 aacaaaatag actattggga caaatggcta tcaacgtaag cacgttaatg agacaaagta
     8941 aggaaagtat tcttttgtca catctttttc acgaaactgc ttgtcagcta agcgaacaaa
     9001 atccggcttt aaccttgatt gatgacatcg aacaattgct tgaaaccttt cgtcatggtg
     9061 atcttcctca aactcgggag gaatttgagc gacgtgcggt tttatttcag ctcttacaag
     9121 acttggaacg ttttatctta ttaaaggtag agttttatca ggattatcaa aatgactaaa
     9181 tctctattgc acacttctta tctatgacag cttgggactt aacttcctga gcttttttgc
     9241 tataataaaa acaagaatct cagaatggag acaagatgaa cgaaataaaa tgccctcatt
     9301 gtcataccct atttactatc aataagtcag agtatagtca attattggaa caagtcagag
     9361 ggcaagcctt tgatgaagaa ttgcaaaaac ggctaatcaa tgagatagcc ttgctagaag
     9421 aaaaagcgaa gcatcaattg catgaggttg tcgcaaaaaa agaaacagct attaccagct
     9481 tgacaaacca attagagcaa attgaaaaag aacaagccta cctcagacaa gaagaactgg
     9541 ctaaaaaaga ccagcttatt gcttctttag aagcgaaact tgataaatta gcctcgcaaa
     9601 atgctttgga actggccaat caattagctg agaaggacaa agaagtggtt agtttgacca
     9661 atcagctgga caaattagca cttgaaaaag atgccacgtt ccagtcaaag cttgctacaa
     9721 tcgaaaaaga acgcgatgga atcaaaaacc aattggcgct tcaagctaaa gaaagtgaat
     9781 tgtcgttggc ttcggtgcgc agcgactacg aagcacagtt aaaagcggct aacgagcaag
     9841 ttgaatttta taaaaatttc aaagcgcagc aatctaccaa agcaatcgga gagagtctag
     9901 aactctatgc tgaaacagag tttaacaaag taagaagcta tgcttttccg aatgctagct
     9961 ttgccaaaga taaccaattg tctagtcgtg ggtcaaaagg ggactatatt taccgagagg
    10021 ttgatgccaa tggtgtagaa atcctctcta tcatgtttga gatgaagaac gaggcagaca
    10081 ccaccaaaac aaaacataaa aatagtgatt ttttcaagga gttagataaa gaccgtcgcg
    10141 aaaaagattg tgagtatgct gttttggtaa gcatgttaga agctgataat gattactaca
    10201 atacagggat tgttgatgtc agtcatgagt atcaaaagat gtatgtggtt cgtcctcaac
    10261 tctttattca gttgataggg attctgcgaa atgcggccct gaatagttta cattataagc
    10321 aagagttggc cttggttaag gaacaaaata ttgacatcac tcacttcgaa gaggatttgg
    10381 accaatttaa gaatgccttt gctaagaact atcagtctgc cagcaacaac tttaaaaagg
    10441 ctattgacga gattgataaa tcgattaaac gcatggagga agtgaaacgc tttttaacca
    10501 ctagtgaaaa tcagttacgc ttagctaata ataaattaga ggatgtttca gttaaaaaac
    10561 tgacccgcca aaacccaacc atgcgagaga aatttgaagc cctcaaggac cagtaagcct
    10621 gaaagtgagt ctttccttgc taaaattggt taagcgttta ttttggcaaa actgatgata
    10681 aagcggtaga atagaatcag taacaattac taattagaga gaaaaataga catgataaat
    10741 ggaatcatta atttaaaaaa agaagccggt atgacctctc atgacgcagt ttttaaatta
    10801 cggaagttat tacaagaaaa aaagattgga catgggggga ccttagatcc tgatgtggtc
    10861 ggtgttctac caattgcagt tggtaaggct actcgtgtga tcgaatacat gacagaagct
    10921 ggcaaagttt atgaaggaca agtgactctc ggttactcaa caacgactga ggatgctagt
    10981 ggagaggtag ttgcgaggtc ctctttgcca gctgtgctaa cagaagaact cgttgatcaa
    11041 acgatgacga ctttccttgg gaaaatcaca cagaccccac ctatgtattc agccgtaaag
    11101 gttaatggcc gtaaattata cgagtatgcg cgtgctggtg aaagtgtgga acgtcctaga
    11161 agagaagtga ccatttccca atttgaacgc accagccctt tgaattttac tgaagacgat
    11221 ctctgccgat tctcttttaa agttgcttgt agcaagggaa cttatgtgcg gaccttagca
    11281 gttgatttag gtcgagctct aggggtagag agtcatatgt ccttcttgca gcgatcagct
    11341 tcagcaggat taaccctcga gactgcctat actttgggag aaattgctga tatggtttct
    11401 aaacaagaga tgtctttttt gttaccgatc gagtatggcg ttgctgatct tcctaaaatg
    11461 gttattgatg atactgaatt gactgaaatt tcatttggtc gccgtcttag tctccctagt
    11521 caagagccac tgttagctgc ttttcatggc gaaaaagtaa ttgctatttt agaaaaaaga
    11581 gatcaagaat acaaacccaa aaaagttctt atttaaatag tttgagtaag tagaatggat
    11641 aattatggaa attgaatata tcaaagatta tcgtgatatt aaccaagaag atgatacagt
    11701 cttggtattg ggatattttg atggattaca ccgtggtcat aaagccttat ttgacaaagc
    11761 aagagaggtt gcaaataaag aagggttaaa agtagtcgtt tttacgttta cagagtctcc
    11821 gaaattggct ttttcgcgtt tttctcctga attattgctt catattactt atccaaagaa
    11881 gcgttatgaa aaatttgccg attacggtgt aaataaatta tatttggttg attttacttc
    11941 taaattctct aaggtctcat ctgatcattt tataactcat tatattaaaa acctgaaagc
    12001 aaagcatatt gttgtcggtt ttgactataa gtttgggcat aatcgtacag atagtgatta
    12061 tttaaccaga aattttgaag gacaagttta taccattgag gaaataaaag aagatcatcg
    12121 aaaaatttct gctacatgga taaggaagct tattcaagaa ggtaatgtag ttaaagcaaa
    12181 ccacttgtta ggctatgact tatcgacaag ggggagagtc gttcacgggg atgcgcgtgg
    12241 ccgtactatt ggtttcccaa cagccaatct agctccaatt gacaacacat acttaccagc
    12301 tgatggagtc tatgttacaa atgttattgt tgctaataaa atctatcgct ctatgacgag
    12361 tttaggtaaa aatgtgacat ttggggggaa agagttgcgt ctagaagtta acatttttga
    12421 ctttgacgaa gagatctatg gagaaattat tgagattgtt tggttagata agattcgaga
    12481 catggaaaaa ttcgagggaa tcgaagattt gactgataga ttggaatatg ataaacgaac
    12541 agcattaaat tggaaaaaag atagcaaatt atcttaaatt tttgtataat aaggtaagta
    12601 aatttgatag aggaaaaata tggttacctt atttttatca cctagctgta ccagttgtcg
    12661 taaagcaagg gcatggttag taaaacacga agttgacttt caagagcata atattattac
    12721 aagtccatta agccgtgatg aattaatgtc aatcctctct tttacagaga atggaacgga
    12781 ggatattatt tcaacacgtt caaaggtttt tcaaaaactt gatattgatg tagaagagct
    12841 ttcaatttca gatttaattg atttaattgc taaaaatcca agtcttcttc gtcgccctat
    12901 tatcatggat caaaaacgca tgcaaattgg ttttaatgaa gatgaaatta gagcgttttt
    12961 atcgagagat tatcgcaagc aagaactacg tcaagcaacg ataaaagcag aaattgaggg
    13021 gtgatatgtc aggtaattat tattatccac ttgacttaag ttggagcact gaagaaatat
    13081 cctcagtgct tcattttttg aataaagtgg aattagcata tgaaaaaaaa gtagatgcaa
    13141 aacaactact agatagttat aagacttata aaaccattgt caaaagtaaa gctcaggaaa
    13201 agcaaatcga ccgtgatttt caaaaagtta gtggttattc gatctatcaa gttgttaaaa
    13261 aagcgaaggc aattgagaaa ggattctttt cccttggaaa ctaaatacgc atttgcaagg
    13321 caaatcatta aagaagcagg gctttttatc aagtcaaaaa tgtcagagca gctagatatt
    13381 caggtgaaaa cacaatttga tgatctggtt accaatgttg atcaagaaac tcaacagtta
    13441 ctgatggatc gtattcatca gacctatcct tgtgatgcca ttttagctga agaaaatgac
    13501 gttcgtcacc ccattaatca aggaaatgtt tgggtcatcg atcccattga tggaacggtt
    13561 aactttattg tgcagggatc acagtttgca gtgatgattg cctattatga acaaggaatt
    13621 ggccaatttg ggcttattta tgatgtgatg gcagatcaat tactagctgg tggtggtgat
    13681 tttgaagtta ctttaaacgg tgataagctt ccagcttacc aagaaaaacc tttagagcgt
    13741 agcttaattg gctgcaatgc aggcatgttt gccagaaatg atcgtaacct tgctcatcta
    13801 attgcaaaga ctttaggtgt aagggtttat ggtggtgcag gtatctgcat ggttaaggtc
    13861 atgaagcagg aattactggc ctatttttca tttatccaac catgggatta cgcagcagca
    13921 aaagtactag gtgataaact gggttatgtt ttactaacta ttgacggata tgaacctgat
    13981 tttcaaacac gtcaaaaaat catgtttgtt cctaaatgcc aactcactag gatagcgtct
    14041 tttctaacaa aggactctgc cgtcatactt gaagatcaat agaaatgatg tcattaccaa
    14101 aagaatttat taacatttat caagcaatac taggcaaaga attagaagat ttcttggcaa
    14161 gttttaacca agagccggtc aatgcttttc ggattaaccc cttaaagaat caactaaaaa
    14221 cctttgaaca ccccattcca aataccttat ggggatatta tggtaaacta tctggcaagt
    14281 ctccagagca tgtgtcgggg ttggtttatt cccaagaacc agcggctcaa atggtggcac
    14341 aagtagcggc tcctcaaaag gagagtcgtg ttttagactt ggcagctgca ccaggaggaa
    14401 aatcaactca tttattagct tatttagaca atacaggtct tttagtcagc aatgaaattt
    14461 ctaagaagcg cagcaaggtt ttagttgaaa atatcgaacg ttttggcgct agaaatgttg
    14521 tggtgactaa tgaatccgca gatcgtttag ccaaggtatt ttctcattat tttgatacta
    14581 tcgtctttga tggaccatgt tctggtgaag gaatgtttcg caaggaccct gatgctattc
    14641 aatattggca tcacgggtat cccgcagaat gcgccaagtt gcaaaaatct attttagaag
    14701 acgccttaac catgttaaaa ccaggaggag aattgatata ctccacctgt acttgggctc
    14761 ctgaagaaaa tgaagatgtt gtgcagtggc tcttagagac ttataccttt ttagaattgg
    14821 tcgatgttcc aaaactaaat ggaatggtta gtggtatcgg acttccagag actgctagaa
    14881 tgtaccctca tcgttatcaa ggggaagggc aatttgtggc caagctaaaa gataagcgtc
    14941 aagaaggaca aagcactaaa ctaaaagcac ctaaatccaa cttgaccaaa gaccagttac
    15001 gtctttggaa aatgtttgaa aaagaccatc taaaccttac cctatcagga acactacaaa
    15061 cctttggtga ttacctatac ctcttgccag atggtcttcc tgctttagat ggccttaaaa
    15121 ttgctcggaa cggtttagaa ttaggcgtct tcaagaagaa acgctttgaa ccctcttacg
    15181 ccttgggatt agctttaaag tctgatgagg tccgatcatc tattgagctt agagaggaag
    15241 actttagacc ttatgtgtca ggtaacgtca ttactttgcc ccaatcttat ccaacaggct
    15301 ggtaccaatt actcattaat ggcaatggtc taggatttgc caaggtcgtt gacaagacca
    15361 tcaaaaataa tttccctaaa gggttaagat tttagcgtaa aaaataagat taaaatgtta
    15421 aatatttaaa gaaaaaatgc tttcaaagca taggtagata tgttactata aagaatatgt
    15481 ggcacttagt ttctacatat atacctttta tttttacgaa taggaaataa ataatgaaaa
    15541 tgaaaaaaaa attctttttg ttaagtcttt tagccctatc aactttcttt ttatccgcat
    15601 gttctagctg gattgataaa ggcgagtcaa taaccgctgt aggatcaaca gcactacaac
    15661 ccttagtaga agcagtagct gatgaatttg gaagcagtaa tctaggtaag actgtcaatg
    15721 ttcaaggtgg tggttcaggt acagggttgt ctcaagttca atcaggagct gtccaaattg
    15781 gaaatagtga tgtctttgca gaagaaaaag atggtattga tgcttctaaa ttagttgatc
    15841 atcaagtagc tgttgcagga cttgcagtta ttgccaatcc taaagtcaag gtttccaatc
    15901 tcagtagtca gcagttgcaa aagatttttt caggagaata taccaattgg aaacaagttg
    15961 gaggagaaga tcttgcgatt tcagtgatca accgagcagc aagttctggc tcacgagcaa
    16021 cctttgacag tgttatcatg aaaggggtca acgctaaaca aagtcaagag caagactcca
    16081 atgggatggt taaatcgatt gtttcacaaa caccaggtgc catttcttac ctttcctttg
    16141 cctacgttga ttcatctgtt aaatctttgc aattaaatgg gtttaaggca aatgctaaga
    16201 atgtggctac aaatgattgg ccaatctggt cctacgaaca catgtacacc aaagataaac
    16261 caacagggtt gaccaaggaa tttcttgatt atatgttttc agatgaagta caacagaaca
    16321 ttgttacaca tatgggatat atttcgataa atgatatgga agtggtcaaa tctcatgatg
    16381 gaaaagtaac aaaaaggtaa tggaggaatt atggaaaatc aggaattggc taaaaaatta
    16441 gcctcaccat caaagaattc aagacttgaa acctttggcc gcaccattac gtttttgtgt
    16501 ctggctttga ttgtttttat cgttgcgatg attctgatct tcgttgctca aaaagggttg
    16561 tcaaccttct ttgttgacaa ggttaatctt tttgatttct tgtttggaaa ggagtggcaa
    16621 ccaagtgtga aaaatgcggc cggcattcct tatcttggtg ctctgccaat gattacagga
    16681 tcctttttgg ttaccatttt atctgctatt attgccaccc catttgccat tggcgcagcc
    16741 gtttttatga ctgaaatctc acctaaatat ggcgctaaat tattacagcc tgcggttgag
    16801 cttttggttg gaattccttc ggttgtttat gggtttatcg gtttgcaagt gattgttcct
    16861 tttatgcgct ctatctttgg tggcacaggt tttggaatcc tatctggggt ctgtgtcttg
    16921 tttgttatga ttttaccaac agtgactttt atgacaacag acagtctgcg ggcggtgcct
    16981 cgtcattacc gcgaagcgtc tatggctatg ggagcaacac gttggcaaac catttggcgt
    17041 gttgtcctta atgcagctcg tccaggaatt tttaccgctg ttatttttgg aatggcgaga
    17101 gcttttggag aagccttagc cattcaaatg gttgtcggta actctgctgt aatgccaagt
    17161 tcactaacga caccagccgc aaccttaacg tctgtcttga caatgggtat tggtaacacg
    17221 gttatgggaa cggtccaaaa taatgtgctt tggtcccttg ccctagtctt attattaatg
    17281 agtctggcct tcaattccct tgtcaaatta atcacgaaag aaagaaagag aaattatgaa
    17341 cgctaaaaaa gtcgataaag tagcaactgg tactctttat atcattgctg gaattattgt
    17401 agctatttta gcttccttaa ttctatatat ccttgtccgt ggcttgccac acatcagctg
    17461 gtccttttta acaggaaaat cgtcttcgta cgaagctggt gggggaattg gtatccaatt
    17521 gtataattcc ttcttcctat tgattgttac cttgattatt tccattcctt tatcaactgg
    17581 agcggggatt tacttggctg aatatgccaa aaaagggcct gttaccaact ttattagaac
    17641 ctgtattgag attctgtctt ccctaccatc tgtggttgta gggctctttg gttacttgat
    17701 tttcgttgtg cagtttgaat atggcttttc tattatttca ggggctcttg ctttgacggt
    17761 ctttaatctt cctcaaatga cccgtaatgt tgaagatagt ttactgcatg ttcatcatac
    17821 ccaaagagaa gcaggattag ctttaggcct gtcacgctgg gaaacggttt tttatgtggt
    17881 tatcccagaa gctctcccgg gaatggtaac aggtattgtc ttagcttcag gtcgtatttt
    17941 tggtgaagcg gcagcgctta tctatactgc tggtcaatca gcaccagctc tggattggtc
    18001 aaattggaat ccacttagtg ttactagtcc aatctctatt ttccgtcaat ctgagaccct
    18061 tgctgttcat atttggaaag tcaatagcga agggacaatt ccagatgcta ctcttgtatc
    18121 cgcaggaagt gcagcagtgc tattaatttt tatccttatt ttcaactttt cagctcgctt
    18181 tattgggaag aaacttcatt ctacaatgac agcagcgaaa taagatattg gagaaatcat
    18241 gacagaatac aattggaatg aacgtcatat catcactttt cctgaagaaa cgcttgctct
    18301 agctaccaag gatttacatg tttactatgg tgcgaaagaa gctatcaaag ggattgatat
    18361 gcagtttgaa aaacataaaa taacagcctt gattggtcca tctggttgtg gcaaatcaac
    18421 ttatcttcgt agtcttaacc gcatgaacga taccattgat attgctcggg ttacaggtga
    18481 gattctttat cagggcattg atgttaatcg aaaagacatg aatgtttacg agattcgcaa
    18541 gcacttagga atggtttttc aaagacccaa tcctttcgct aaatccattt ataaaaacat
    18601 tacctttgcc catgaaagag ctggtgtgaa agacaaaaaa gttttagatg aaattgttga
    18661 gacttcactg aaacaagccg cgctttggga tcaagttaaa gatgaccttc acaaatcagc
    18721 cttcacctta tcagggggtc agcaacaacg cttgtgtatt gctagagcca tttctgtcaa
    18781 acctgatatt ctattaatgg atgaaccagc ctctgccctt gacccgattg caacgatgca
    18841 gctggaagaa accatgtttg aattgaagaa gaattacacg attattattg tgacccacaa
    18901 tatgcaacaa gcagctcgtg ccagtgatta cactgccttc ttctatttag gcaacctcat
    18961 tgaatatgat aagacacgta atattttcca aaatgcgcaa tgtcagtcaa caaacgacta
    19021 cgtttcaggg cactttggtt agaaaggtaa tatgatgaca gaacctattt tacagatcag
    19081 agacctttct gtttactaca atcaaaagaa aacgttaaaa gatgtctctt tggaccttta
    19141 tccaaatgaa ataacagctt taattggtcc ttctggatct gggaaatcaa cgctcttgcg
    19201 ttctatcaac cgtatgaatg atttgaatcc tgaagtaacc attacaggct ctatcgtcta
    19261 taacggacac aatatttata gtccaaggac agatacggtt gatctacgca aagaaattgg
    19321 tatggtattc caacaaccca acccatttcc gatgtctatt tatgagaacg tggtttacgg
    19381 ccttcgttta aaaggtatca gagataaatc aatcttagac catgctgttg aaagctctct
    19441 taaaggggct tccatttgga atgaagtcaa agatcgttta catgattctg cggttggctt
    19501 atcaggcgga cagcaacaga gagtttgcat agctagagtt cttgctacca gccccagaat
    19561 aatcctttta gatgaaccta cctcagctct tgatcctatc tcagctggga aaattgaaga
    19621 aacattattg ttattaaaaa aagattatac actggctatt gtgacccgct caatgcagca
    19681 agcctcacgt ttatcagatc gaacaggttt tttcttggag ggtgacttac ttgagtgtgg
    19741 tcctacaaaa gccatgttta tgaatcctaa gcgcaaagaa accgaagact atatttctgg
    19801 taaatttggt tgagaaagac gtatatggtt gacccctctg tgtttttatt ataatgacta
    19861 gattgtagga agaaaaaatc atgttaagaa cgaaatttga agaagaatta gataaattgc
    19921 ataaccagtt ttattcaatg ggaacggaag tattggcaca aatcaataaa actgttcgtg
    19981 cctttgttag tcatgatcgt gaattggcca aagaagttat cgaagaagat gacactatta
    20041 ataattttga gacaaagctt gaaaagaaat cgctggaaat tattgcccta caacagccag
    20101 tttcgaatga tcttagaatg gttattactg ttttaaaggc atcaagtgat attgaacgta
    20161 tgggtgacca tgctgcttcg attgccaaag ccactattcg tatgaaaggt gaggagcgta
    20221 ttccagtggt tgaagaacaa atcaacctta tgggaaaagc tgtgaaacag atggtagaag
    20281 aagcccttaa tgcatatatc aatgctgacg atacaaaagc ttatgaaatc gcagcttctg
    20341 atgagattat cgatcaatat ttccgtaata tccaaacctt agctgttgaa gaaatccgta
    20401 agtctccaga tgctgttttt gcaggaaaag aatattttca agtattaatg tatttggaac
    20461 gtattggtga ctatgctcgc aacatttgtg aatggattgt ttatcttaaa actggaaaaa
    20521 ttattgaatt ataagaaatt atgctataat gagactggga aacagcttgc cagtccctgg
    20581 ttttatacga aattgtgcaa gttagaaaat gaactaacaa gaaaagtttt ctttgctgaa
    20641 tctgattttg ttaggttaac aaatatatgt agacagagat gatacttagg tatcatctgt
    20701 tgatctatta tgatttgtta ttagaattaa ggagttatat gaagacagta gaacatctca
    20761 tcgaaacatt tgtgcctgaa aattataaca tctttttaga tattaatcgt cagactaaaa
    20821 catttacggg gaatgttgcg attaatggtg aagctttaga taatcatgtg gctttccacc
    20881 aaaaagattt ggacattaag tctattttgc tcgataatga agctgttatt tatcaagtgg
    20941 ataacgacaa tgaagttgtg cgtgttgaat tacctgaaac aggaatgatg acgcttgtca
    21001 ttgaattttc aggtagcatt acagataata tgacaggtat ttacccttca tattacacta
    21061 aaaatggtga gaaaaaagaa gtcatttcaa ctcagtttga aagccatttt gcacgtgaag
    21121 ctttcccatg tatcgatgaa ccacaagcaa aagctacttt tgatttaagc ttgacctttg
    21181 atcaagaaat tggtgagata gcgttatcga atatgccaga agtcaatatt gaccgtcgcg
    21241 aggagactgg tctttggaca tttgatacga caccaagaat gtcatcttac ttgctagcgt
    21301 ttgcgctagg agaacttcat ggaaagactg tagaaagcaa aaaaggaaca actgttggtg
    21361 tgtatgcgac aacagcgcat cctcttagct cgcttgattt ttcattggat attgcagttc
    21421 gtgttattaa cttttatgaa gactattttg gagtacatta cccaatcccg caatccctta
    21481 atattgcgct tccagatttt tcatctggtg ctatggaaaa ttggggttta atcacttatc
    21541 gtgaaattta tttattagta gatgaaaatt cgactgttca aagccgacaa caagtggcac
    21601 ttgtaatcgc ccacgagata gcacaccaat ggtttggaaa cttggttaca atgaaatggt
    21661 gggacgatct gtggttaaat gagagttttg ccaacatgat ggaatatgtc tctatcgagg
    21721 ctattgagcc gtcttggaaa ataatagaag atttccaaac aggcggtatt cctcttgctt
    21781 taaaacgtga tgctactgat ggtgttcaat cagtgcatgt tgaagtcaat catccagatg
    21841 agataaatac attgtttgat cctgctattg tctatgcaaa aggtagtcgt ttgatgcata
    21901 tgttacgccg ttttattggt gatcgtgatt ttgccattgg cttacatcac tactttgaaa
    21961 aatatcaata tcgaaatact gtcgggcgtg atttatggaa tattttatca gatacttcag
    22021 gcaaagacgt agctgctttt atggatgctt ggcttgaaca accaggttat ccagttttaa
    22081 cagctcgcct tgaaaacgat caattgattt tgagtcaaaa acaatttttt attggaaaag
    22141 gtgaagaaaa aggtcgcctt tggccgattc ctttaaacac taattggcat ggccttcctg
    22201 aaactttgac agaggcagaa atggtcattc caaactttag tcaattggca gcagaaaatg
    22261 aaggagctct acgtttcaat attgataata cagctcatta cattacagat taccaaggat
    22321 cattattaga tgccttagtc actgaacttg cccaacttga taatacttct gctttacaag
    22381 ttattcaaga acgtcgttta ttggcagata gtggcttgat ttcctatgct gaattggttg
    22441 acttaattgc tcaattggac gattctaagt cttatatggt agcagaagcg gttcagcaag
    22501 tggtctctgg tttgaaacgc ttcattgatg aaggaagctt agcagaaaaa tcctttaatc
    22561 gcttggtaac caccatctat caagaagact ttaatcagca tggttttgag aaaaaagctg
    22621 acgaatcaga tgaagatgag atggtacgtc aggttgcctt gggccgcttg tggctagcag
    22681 aaaatcagac gattattgac ggattgagaa ctatttttga agcatatcaa aacaatattg
    22741 caagtattcc agcagctgtt cgtcgcttgg ttttagccaa ccaaatgaaa tattttgaaa
    22801 cagatagctt ggttgatatt tattttgaga cttatgtagc aactactgat aataatttac
    22861 gcagtgattt aacggttgct tttagtcaaa caaaacaacc aactactatt agacgtattc
    22921 ttgttagttt aaaagataaa gatattatta aacctcagga tttatcttat tggtataatg
    22981 ctttgcttgg acaaagcttt actcaagata tcatttggga atgggctaga gaaaattggg
    23041 attggattaa atctgcactt ggtggagata tgagctttga taaatttgtt atttatccag
    23101 caagtaactt taaaacacca aaacatctag cagaatataa atctttcttt gaacctaagc
    23161 tagatgatat ggctattagc cgtaatatta ccatgggaat taatgaaatt gaagcacgtg
    23221 ttgctttgat cactaaagaa aaagaagcag ttattgcagc tctcagtcat tactagacca
    23281 taagcaaaag agttggggga ctttccagct cttttttgta gtgagctagc ttcctttctt
    23341 agttccgtct ataactaagt gattatctat agtttaattc aaaaggagat tgcactttaa
    23401 agcgaaggct attagcctct taatactttt tttaccaggt ggtgaataag aacaattata
    23461 aatgatttaa tcattatgct ttaaatgcct taaaggagta ttaatatttt agtaaacctt
    23521 aagctttttg tagtatagtc tcgttagaaa ttattatcag aaatgagact aagttaaatg
    23581 aagtgatagt tactttattt aagaacaatt gaggtagaga catgataaag atattattag
    23641 tagaagatga cttgagctta tcaaattcca tttttgattt tttagatgat tttgcagacg
    23701 tcatgcaagt ttttgatggc gatgaaggtt tatacgaagc agaaagtggt atttatgatt
    23761 tgattttgtt agaccttatg ttgccggaga aaaatggctt tcaagtctta aaagaattgc
    23821 gagaaaaaga tattaaaatt cctgtgttga tcatgacagc taaagagggt ttagatgata
    23881 aaggtcacgg atttgaatta ggtgctgatg attatctgac aaagccgttc tatttagaag
    23941 agttaaaaat gcgaattcaa gcactgttaa aaagaacagg gaaatttgca gataaaaata
    24001 ttagttttgg caatttagtg gtcgacttag ctcgaaaaga ggtgaaggtt gaagggaaag
    24061 tagttgaatt acttggcaaa gaatttgatt tgttggtgta tcttttgcaa aatcaaaacg
    24121 ttattttacc aaagacacaa atttttgatc gtttatgggg attcgatagt gatactacga
    24181 tttcggttgt tgaagtttac atttctaaaa taaggaaaaa attaaaaggt acccgttttg
    24241 ttaacaggct tcaaacttta agaagcgtag ggtatattct aaaaaacaat gaataaatta
    24301 aaaaaagaga ttttatcaga taactataac cacttttttc atttttttgc ggtttttaca
    24361 ggtatttttg tcattatgac tattattatc ttacagatta tgcggtttgg cgtttattcg
    24421 tcagttgata gtagtttagt ttctgttagt aataatgcaa gtagctatgc taatcgtacg
    24481 atggctagaa tatcttcttt ttactttgat actgaaaata acattattaa ggcgctgcct
    24541 gattcagata gttctaagtt attaggaacg cctgcagcta atacagatat cattttgttt
    24601 agtgctaatg gaacaatttt aaatgctttt gatgcgtttt ctaactatca aaattttcat
    24661 ttagataaac gccggttggg gagtattgaa accaccagtt taatgaattt ttatggacaa
    24721 gaagaaaaat accatacgat aactgtaagg gttcatatca aaaattatcc tgcagttgcc
    24781 tatatgatgg cagtagtaaa tgtggaacaa ttagaccgcg ctaatgaacg ttatgagcgc
    24841 attattatta tagttatgag tgttttttgg ctaatttcta ttttagcaag tatttattta
    24901 gccaagtgga gcagaaaacc tattttagaa agctatgaaa aacaaaaaat gtttgttgaa
    24961 aatgctagtc atgaactaag gacccctttg gcggtcttac agaatcgtct ggaatcgctt
    25021 tttcgtaagc ccaacgaaac gatattagaa aatagtgagc atctcgcttc tagtttagac
    25081 gaggttcgca acatgcgcat cttaacaact aatttattaa atttagcaag acgagatgat
    25141 ggcattaatc cacagtggac tcatttagat acagattttt ttaatgctat ttttgagaat
    25201 tatgaactag ttgctaaaga atatggaaaa atattttatt ttcagaacca agtcaataga
    25261 tcgttaagaa tggataaggc tttactaaaa caattaata
//
LOCUS       DAUGFP010000023        24569 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.023, whole genome shotgun sequence.
ACCESSION   DAUGFP010000023 DAUGFP010000000
VERSION     DAUGFP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24569
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.023"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            48..1280
                     /locus_tag="VH663_000966"
     CDS             48..1280
                     /locus_tag="VH663_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ8174177.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFDGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1448..2629
                     /locus_tag="VH663_000967"
     CDS             1448..2629
                     /locus_tag="VH663_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ8174178.1"
                     /translation="MTKRIILTIFTFICFSMMPLVHAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFTMGR
                     Q"
     gene            complement(2781..4199)
                     /gene="sufB"
                     /locus_tag="VH663_000968"
     CDS             complement(2781..4199)
                     /gene="sufB"
                     /locus_tag="VH663_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEQ8174179.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4212..4676)
                     /locus_tag="VH663_000969"
     CDS             complement(4212..4676)
                     /locus_tag="VH663_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEQ8174180.1"
                     /translation="MALSKLNHLYMAVVADHSKHPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHSTSRTKM"
     gene            complement(4663..5889)
                     /locus_tag="VH663_000970"
     CDS             complement(4663..5889)
                     /locus_tag="VH663_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ8174181.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(5920..7182)
                     /gene="sufD"
                     /locus_tag="VH663_000971"
     CDS             complement(5920..7182)
                     /gene="sufD"
                     /locus_tag="VH663_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEQ8174182.1"
                     /translation="MTKEKLVAFSQAHAEPAWLQERRLAALEAIPELELPTIERIKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKISANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQETAERLVIRGF
                     LGAVIAEIPIPSVRQEIIKVLDEKLLNR"
     gene            complement(7277..8047)
                     /gene="sufC"
                     /locus_tag="VH663_000972"
     CDS             complement(7277..8047)
                     /gene="sufC"
                     /locus_tag="VH663_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEQ8174183.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADEDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYIIPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8169..9338)
                     /locus_tag="VH663_000973"
     CDS             complement(8169..9338)
                     /locus_tag="VH663_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEQ8174184.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLISAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9345..10106)
                     /gene="mecA"
                     /locus_tag="VH663_000974"
     CDS             complement(9345..10106)
                     /gene="mecA"
                     /locus_tag="VH663_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEQ8174185.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10252..11091)
                     /locus_tag="VH663_000975"
     CDS             complement(10252..11091)
                     /locus_tag="VH663_000975"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEQ8174186.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIVFIWIEKRNAQQEPAVTELARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTDYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11157..13082)
                     /locus_tag="VH663_000976"
     CDS             complement(11157..13082)
                     /locus_tag="VH663_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEQ8174187.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRIGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKTLEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGKEPV
                     DVSTSKKNKIVALETKISRRRTLLQLLFGKVIPLVEVGFLLSQLIQFTRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLTQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASAAITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSNSVQGLGCLLLILNSGLLIYLAIKESGLALIYLALMVLTMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFVSSLSSATTSSNFSVSSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            13280..14848
                     /locus_tag="VH663_000977"
     CDS             13280..14848
                     /locus_tag="VH663_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ8174188.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKIAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYAEATSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGAIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAIAS
                     FLGQMWAIFKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRYIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            14841..15581
                     /locus_tag="VH663_000978"
     CDS             14841..15581
                     /locus_tag="VH663_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174189.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDRIIFMDKGL
                     IAEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            15723..15839
                     /locus_tag="VH663_000979"
     CDS             15723..15839
                     /locus_tag="VH663_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEQ8174190.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(16065..17075)
                     /gene="gap"
                     /locus_tag="VH663_000980"
     CDS             complement(16065..17075)
                     /gene="gap"
                     /locus_tag="VH663_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEQ8174191.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINSAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(17415..19493)
                     /gene="fusA"
                     /locus_tag="VH663_000981"
     CDS             complement(17415..19493)
                     /gene="fusA"
                     /locus_tag="VH663_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEQ8174192.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(19977..20447)
                     /gene="rpsG"
                     /locus_tag="VH663_000982"
     CDS             complement(19977..20447)
                     /gene="rpsG"
                     /locus_tag="VH663_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEQ8174193.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(20468..20881)
                     /gene="rpsL"
                     /locus_tag="VH663_000983"
     CDS             complement(20468..20881)
                     /gene="rpsL"
                     /locus_tag="VH663_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEQ8174194.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(21089..23710)
                     /locus_tag="VH663_000984"
     CDS             complement(21089..23710)
                     /locus_tag="VH663_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEQ8174195.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIDAVEKTLSQQKAELTELATALTKTTAEINNLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKA
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEALAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQEALAALQAKQSSLEATIATTEHQLTLLKTLANEKEYRHLDED
                     IATVPDLQVAPSLTGVKPLSYSKVETTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESR"
     gene            complement(23701..24540)
                     /gene="purR"
                     /locus_tag="VH663_000985"
     CDS             complement(23701..24540)
                     /gene="purR"
                     /locus_tag="VH663_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEQ8174196.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
BASE COUNT         7283 a         5189 c         4514 g         7583 t
ORIGIN      
        1 atatggtata attaaaaaaa taatacttga gtaaggataa tctataaatg atcaaacgat
       61 taatttccct agtggtcatc gccttatttt ttgcagcaag cactgttagc ggtgaagagt
      121 attcggtaac tgctaagcat gcgattgccg ttgaccttga aagtggcaaa gttttatacg
      181 aaaaagatgc taaagaagtt gtccctgtcg cctcagtcag taagctcttg acaacctatc
      241 tggtttacaa agaagtttct aagggcaagc taaattggga tagtcctgta actatttcta
      301 actaccctta tgaactcact acaaactata ctattagtaa cgttcctctt gataagagaa
      361 aatataccgt taaagaactt ttaagtgcgt tagttgttaa taacgccaat agccccgcta
      421 ttgctttagc tgaaaaaata ggcggaaccg aacccaaatt tgttgacaaa atgaaaaaac
      481 aattaagaca atggggcatt tccgatgcaa aggtcgtcaa ttcaactggc ttaactaacc
      541 attttttagg agctaatact tatcctaata cagaaccaga tgatgaaaat tgtttttgcg
      601 ccactgattt agctattatt gccaggcatc tcttattaga atttccagaa gtactgaaat
      661 tatctagcaa atcctccact atttttgatg gacaaaccat ttacagttat aattacatgc
      721 ttaaaggcat gccttgttat cgagaaggcg tggatggtct ttttgttggt tattctaaaa
      781 aagccggtgc ttcttttgta gctactagtg tcgaaaatca aatgagggtt attacagtag
      841 ttttaaatgc tgatcaaagc cacgaggatg atttagctat atttaaaaca accaatcaat
      901 tgttgcagta ccttttaatt aattttcaaa aagtccagtt aattgaaaat aataaaccag
      961 taaaaacgtt atctgtctta gacagtcctg aaaaaactgt caaacttgta gcccaaaata
     1021 gtttattttt tatcaaacca atacatacaa agaccaaaaa taccgtccat attactaaga
     1081 aatcatccac aatgatcgca cctctatcaa agggacaagt cttaggtaga gcaacccttc
     1141 aagacaaaca tcttattgga caaggttatc tggatactcc tccttctatc aatcttatcc
     1201 ttcaaaaaaa catttctaaa agtttctttt taaaggtctg gtggaaccgt tttgtgaggt
     1261 atgtcaatac ctctttatag aataacacaa tgctttttgt atgacattga ctgacagagt
     1321 acttctgcag tctttttcat ttttatttaa gcaaactagg ctaatcctaa caatttttca
     1381 aaaaaacttc acattgtatt agcaaagtga tatactgaaa atgcattaca tttaaaggag
     1441 tcggaaattg accaaaagaa ttatactaac tatttttacc tttatttgct tttcgatgat
     1501 gcctcttgtc cacgccgaag atgtcatgga tattacaagg caagcaggct acacagttag
     1561 tgaggttaat cgaccaaaat cttctattgt cgttgatgca aatagttcag atattctttg
     1621 gcaagataat attgatattc ctagggatcc tgctagtatg tctaaaatgt ttacgcttta
     1681 cattttattt gaagagctcg ctaagggaaa aattacgatg gacactacta ttacggcaac
     1741 tccaaccgat caagctatcg ctaacattta tgagattagc aataataata tcgtcgctgg
     1801 agtagcttat cctattcgcg atcttattac tatgacagct gtgccttcat caaatgcagc
     1861 aactgttatg attgccaatt atttatcaaa caatgatgct tctgcgttta tcgatcgtat
     1921 taatgccaca gccaaacaat taggcatgac taacactcat ttttcaaacg ctagtggtgc
     1981 agcagcacaa gcttttcaag gttattataa tcctactaaa tatgatttat ctgcttcaaa
     2041 tattacgacc gctcgagatt tgtccaagtt actctatgcc ttcttaaaaa aataccctga
     2101 aattatctcc tttacaaata aatctgttgt gcacactatg gtaggaacgc catatgaaga
     2161 agaatttcac acctataacc actctctccc agacaatcaa tttggtatga aaggagttga
     2221 tggattaaaa acaggttcta gtcccagtgc tgcttttaac gccatgataa ctgccaaaag
     2281 aggcaaaact cgactcatta ctattgtcat gggggtcgga gattggtcag atcaaaacgg
     2341 agagttttat agacatcctt tcgtcaatgc cttgactgag aaagggttta aagatagtaa
     2401 gaccttatcc aaaaaagcac gccaaaaact cgaaaaatta gtgccacaaa ccaaaaaaga
     2461 aacatcacct aaacagcagc acttcaaagc cactaaaaag caatcgtact tggaaagagt
     2521 cgaagatttt atgaatcaca accacacttt cttgcttatt tgcttagcta tctttataat
     2581 tactatcctt ttactaagcc tcgttgtctt tacaatggga cgccaataat gaattatcaa
     2641 aaacctctgt caaagcacag aggtttttga tatgttatct tacgtgttta aggcaactaa
     2701 aaactaacca cttggggtta gtactacaac tcataaaatc ctatctcaaa gatctttcag
     2761 atggataaga gaaaatgcaa ttaaccgaca gaaccttcca tctcatagga aatcaatcga
     2821 ttgagttcga ccgcgtattc cattggcaat tctttggtaa aaggctccac aaatcccatg
     2881 acaatcatct ctgtggcttc gctttctgat aagccacggc tcatgagata gtagagttgt
     2941 tcttcagaaa tcttagacac tttggcttca tgttctaacg ccacctgtga attatgaatt
     3001 tcgttaaatg ggatcgtatc tgatttagaa atatcgtcca tcaaaatagt atcacattca
     3061 atatgggaaa cggatttttt agattgcttg ttaaaagtca cttggccacg ataatctact
     3121 ttaccaccag acttggcaat tgattttgac acaattgatg atgatgtatg tggagcattg
     3181 tgaatcattt tagcccctgt atcttggtgt tgacctgcgt tagcaaaggc aatagacagc
     3241 atggtgccac gcgcacctgg cccatcaaga taaacagaag ggtacttcat ggtggtttta
     3301 gctcctagat tgccatcaat ccattccact gttgcatccg taagggcgcg tgcacgtttt
     3361 gttactagat tatacacatt gtctgaccag ttttgaatgg tggtataacg catgtatgca
     3421 ccgtcaagcg caaagatttc aacgatagca gcatgtaagc tgttacttga ataagtggga
     3481 gctgtacatc cttcaacata atgaacactt gctccttcat caacaataat caatgtccgc
     3541 tcaaattgac cagtattttc attgttaatg cggaaataag tttgcaaagg gatatctacc
     3601 ttgacccctt taggaacata aataaaggta ccaccagacc aaactgctga attgagggct
     3661 gctaatttgt tgtctgttgg cggaaccagt ttggcaaagt attgtttgaa caaatcagga
     3721 tattctttga gggcggaatc ggtatctgta aagataatcc ctagcttttc aaattcaccc
     3781 ttcatgttat ggtaaaccac ttctgactca tactgagctg atgctccagc aagataagca
     3841 cgttctgctt ctggaattcc aatacgatca aaggtttctt ttattttttc tggaacatca
     3901 tcccaagaac gagctggctt atcagatgct ttttgatagt aaatgatatc atcaaagtta
     3961 atatctgata agtctgctcc ccaggtttgc atcggcattt tattaaaggt ttccaaggat
     4021 tttaaacgaa actccaacat ccactcaggt tcatttttgg cagctgatag ttcgcgaacc
     4081 actgcctcac tcaatccttt tcctgtagaa taaatgggct ctacatcatc gtgaaatcca
     4141 aattggtagt cccctaagtc aattggcttt ggctctactt tctcatttat atcagacatt
     4201 tttcccttcc tttacatttt ggtccgtgag gtgctgtgct gagcattggc acttcgttta
     4261 atggcttcct tgagagcatt ccaagctagg gtagagcatt tgatacgctg tggaaatttt
     4321 gcaactcctg ccaacaattc tgcttcacct agctcttttt gagcaggatt ttcctgtcct
     4381 tgtaccatct ctgaaaaaat atcagctaac gcgagtgctt cttctttact tttaccaata
     4441 acagcatctg tcatcatgct tgatgaagct gtggaaatgg tacagccgtt gcctgcaaaa
     4501 gcaatatctt caattttatc ttcgtcaaac ttaacggtca aagaaatcac atcaccacaa
     4561 gtcggattat tcagttgaac agcctctacg ccatctagtt gcccatgatg atgtggatgt
     4621 ttcgaatggt ccgctaccac agccatgtat agatggttca gtttagagag tgccattgaa
     4681 aaactccttt gcttttagaa ttgcttcgac taaacggtca caatctgcct tggtgttata
     4741 gatataaaaa cttgctctaa cagttgctgg tacacctaaa taactaagaa gaggttgggc
     4801 gcagtggtgc cctgctctta ctgcaacacc ttcatagtcc aaggctgttg ccaagtcatg
     4861 aggatgcaaa tcatccagat taaaagaaat cagaccagat cttgcactag gctggcttgg
     4921 tccatatatg gtaagcccct caatagcttc taatttcggc aaaacatagg ctattagttc
     4981 tgcttcatgc gcatgtatat cagccatgcc tagtctctga aggtaagaaa tggctgcgct
     5041 tagcccaata gcaccagcta tgtgaggtgt tcctgcttca aacttccagg gcaattcttt
     5101 ccaagtggcc tcttgttcgt aaacaaaatc aatcatttct ccgccaaatt caagaggctc
     5161 cacttgattc aaaagctctt ctttgccgta aagaacaccc aaacctgttg gccccaacat
     5221 cttatgagct gaaaaagcaa agaaatcaca atccaagtct tgaacatcaa tagccaaatg
     5281 tggaaccgac tgggcaccgt caacaacaag gtaggctcct ttagcatgtg ccagcttggc
     5341 aatttcttta atgggattga tgcaaccaag aacattggag acatgtacta ggctaacaaa
     5401 acgtgttttt gtcgtcaatt tgtttgccaa atcgtccatg tcaagttggc catcttttaa
     5461 ataaacatag actaaccttg ctcctgtttt ttgacaggct tgttgccaag gaatgatatt
     5521 ggcatgatgc tccataatcg aaatcaacac ctcatcttct ggcgtcaaga cctgctctgc
     5581 aaaccgagca acccaattca aactggttgt tgttcctctg gtaaagagca cttccttgct
     5641 tgatttagca tgaataaagt cagcaacctg ctggcgactg gcctcatatt tgtgcgttgc
     5701 acgttcagcc aaggtatgaa ctcctcggtg aacattagca ttatcttctt gataatagga
     5761 ttgcaaagcc tcaagaacaa gcgccggttt ttgtgtggtg gcggcattat ccaaataaac
     5821 aaggggttca tcattgactt gttgatttaa gatttgaaag tcttgtttga tgtcttttgc
     5881 gtctagcaat actaacctct ttctttttgg cagtaggtct taacgattaa gcaatttctc
     5941 atctaaaacc ttaataatct cttggcggac tgatggaata ggaatttcag cgataactgc
     6001 tcctaggaat cctctaataa ccaatcgttc tgctgtttct tgatccagtc ctcgactcat
     6061 caagtaatac atatcttcag ggtcaacctg accgatagaa gctgcatgac ctgctgttac
     6121 ttcattttca tcaattaaga ggattggatt ggcatcggct cttgcttggt cagaaagcat
     6181 caaaacacgg ctttcttgtt gagcatcagc tcccttagcg ccttttagaa tatgaccaat
     6241 cccgttaaac gttaaggtgc cacgttccaa aatcacacca tgctgtaaaa tatgaccgac
     6301 cgtacgttga ccatagttgg tcacgcgcgt gtcaatacct tgtacctgac gaccacttga
     6361 ggctgcaaca actttcaaat cagcttgtga gccctgacca atcaaatcac tgtcaaaatc
     6421 agcaatgaca ttgccttcat tcatcacagc taaggcccaa tcaatgttgg catccttctc
     6481 taaacgtcct cgacggctaa tataggttgt cactgaagga cctaagcggt cgatagccga
     6541 gaatttaatc tggctgcctg cttgagcaat cacttctaca ctgatattag cgctgatctt
     6601 ttgagtggca ttgccaatag attcaaaacg ctctaaatag gtgaacttac tttcttttcc
     6661 tgcaatcact agaacatgct tgttaaaagg aacgtcactg tcactatctt gtaagaaaat
     6721 agcttcaata ggagttgtga tttccaagtg atcaggaacg tagagcacgg ctgcgctatt
     6781 aaaataagca gtgtggtagg cagctagttt gtcttcatca aaagctaatg cctgaccaaa
     6841 atgagcttca attacttctg ggatttcctc aagcgccgta taaaaatcac tgaaaacaac
     6901 tcccttgtca attaacgcca ttggtaactg ttctaagact gtttgcgtgc ctacctgaac
     6961 aagctttggg ttatctccaa tagctataaa atctggaaca ctagctagac tttcattttc
     7021 tgttaaggta ccatctccta gattccaacg gtgaaattta atcctttcga tggttggtaa
     7081 ctctaattct ggtatggctt ctaatgccgc taaacgccgc tcttgcagcc aagcaggctc
     7141 agcgtgggct tgcgaaaaag ccactagttt ttcttttgtc atctgtcaat cctttgtctc
     7201 tggtttttaa attggctggg tatatagaac atcacctagc tttttgtaga aagctaaagt
     7261 aattgtctcc aaaaacttaa ctttcttcct tgtactcgat tcccagatcc tgtgcaatac
     7321 ctgcgtagcc ttctttttca agacgagtag ccaaggcagc atcaccagat aagacaatac
     7381 gaccatccat cataacatga actaggtctg ggataatgta attcaacaaa cgttgatagt
     7441 gggtgataat catagctcca aagtctttcc cacgcatctc attaacccct ttagagacta
     7501 cttttagggc atcaatatca agaccagaat caatctcatc taacaaagca aatttaggct
     7561 ctaacatgag caattgaaga atttcattgc gttttttctc cccaccagaa aaaccttcat
     7621 tgaggtaacg ctctgccatc tcttctttca tacctaacaa agccattttc tcatcaagtt
     7681 tggtgataaa atctcgcacg gaaatcttat cctcatctgc cttaccggca ttcattgctg
     7741 cacgcataaa ttcagcgttg gtaatccctg gaatttctga agggtattgc atggcgagaa
     7801 aaagtcccag tctcgcacgc tcatcaactt ctagatcgag aatattgacc ccgtcaagca
     7861 aaatctgccc ttgggtcact tcgtagttag gatttcccat aatagctgct gaaagggtag
     7921 actttcctgt cccatttggc cccataatgg cagctacttc cccagtttta agagttaggt
     7981 taaccccttt caaaatctct ttaccttcta ttgaaacatg taaattatta atctcaagaa
     8041 ttgacatgat acacccctta ttttctctgt catctatacc atctcaagaa aagacagtgt
     8101 catttgcact gcttttttta taatggtcac tgcatctatt atagcaaaaa aagccttact
     8161 cggctttttt agtgtttcaa atccttcttt tctttccatt tcagaagcat tgcttgacga
     8221 taatcactat taccaataaa ctttaacaag ttaaacaaag gcgttcgctt ttcaccccag
     8281 atctccaatc cttcaataaa aacttctaaa ccaaataaaa gtccaagcat caagaggacg
     8341 ccaccaattc gactagaaac atttaaaagt aaagaaataa gggaaaaaag catggtaata
     8401 ccataaacta ctaagacagc tcctcgatga gtaaagccca ttgataagag tcgatgatgc
     8461 aggtgcatct tatccggctc gtagaatttt tgacctgata aactacgtcg aataatagcc
     8521 acgatggtat ccataatggg tacaccaaga ataataacag gtgtcaccac agccacagcc
     8581 gttgaatttt tcaatccttg aagagacaaa acccctatca taaagccaat aaagagggct
     8641 cctgtgtcac ccaaataaat catagctgga tggtaattat aaggaaaaaa tcctgcaata
     8701 gcagaaatca agaccaaaat ggttaacgtc aaaaagaaat ccttttgagg taaaaagaaa
     8761 taagatacaa tagccatggt cactaaacta ataatagaga ccccactaac caaaccatcc
     8821 aaaccgtcaa tcaagttaat agcattagtg atggaaacaa tccacaggac tgttaagaat
     8881 aaggttaaga aaggaccaaa aactaacaac ggccctccaa aaggaatttt gaagctatca
     8941 aatttaaagt cggtaaaagc ccagacaatc actgcaccaa ttagaatacc aaacatcttt
     9001 aatttaggac ttaactcata caaatcatct aaaaaaccag taagggtaat tactgtagca
     9061 ccaatcacga caggtaaaat ataactgaag taggtctgac caaaaaacat ggcgccttta
     9121 gaagcaatcg gaataagtcc caaacttgct actaaaaaag acataaaaat agctaatcca
     9181 ccactggttg gcatcggaac cttattgacc cgtctggcat tgggattgtc tactgcaccc
     9241 acacgaaagg ctaaaaagcg aacaagaggg gttaagaata aggacattaa caaagcccct
     9301 atcagaacaa ggacataatc tattgtgaat gaaaacatac tattttatcc caactcaatc
     9361 ttttgtaatt gctcaacagc accatcaagc atcaattgta agccatgctc ttgtaaataa
     9421 gctcttgttt ttgatccagg gtttgcatgc tcaatcaatc tcgcatacat gacattcgca
     9481 aaataagatg gttgttgctg aacatctaat aaaatagtca tatggtagca gttgctcccc
     9541 ttatataact ctgaagcttc tatcgaaaaa tcaatcgttt tagcaaaagc cactgcctct
     9601 gtaatcgttg aaaaatccaa tacataatgg acatagtcta aaggttctga ctcatgatta
     9661 gttgatggat cagccgcctc agactgatta gctagagtgg cttcaacaac atcttccatt
     9721 atctcttcaa ttttttccaa tttttcatga gctttgacat ctcccttttc acgcatggat
     9781 tgctccaagc tcttgaaaaa atcttccgga gtcatttggg acatatcacc aaactctgct
     9841 aaatcttcaa gattaatatc tttatttatc tcagatttgg taacaaaaac atctagacgg
     9901 tccttgcgtg gagtcactcg aaaactaagc ataccactat ctttaaaatt atctggaaga
     9961 tcgagttcgt ccatcacaga atagaaaaac tcttctgttt tttcttgagg aatcaagaaa
    10021 tctttcaact ccattcctct ttcttctaaa tcgtccatac taattgttat ttttagcgtt
    10081 gtctcgctaa tttgtttcat ttccattctc aatacctcgc acgcgtagtc attttattat
    10141 actagaaatg ctgtaatttc gcaatttatg actaactcct cttctacaaa aggaaattta
    10201 aaaagcgaca gtccccaagg tctgtcgcaa catcctaaac gatatgtaag tttaaaaaac
    10261 aaatttaaag aagctataaa taagtaaaag gcttcctaac acaatacggt atttcccaaa
    10321 aatggtgaaa tcgtgtttct taacataatc tgtcaagaac ctaatcgcca ataaactaac
    10381 cacaaaagcg gtcaagctag ccactaaaag aataaggact tgtgcaaaat caaggtgatg
    10441 gccatcaagg aaaaatttca cagcctttaa tccactatat ccaaacatgg taggaatggc
    10501 caaaaagaag gtgaaatcag cggccactgt tcgactagtt cctaaaataa tagctcctaa
    10561 gatggtagcc cctgatcgac tagtaccagg cacaatactt aagacttgaa aacaaccaat
    10621 gaagaaagct gttttgtaag acattctagc taattcagtg acggcaggtt cttgttgagc
    10681 atttcgtttt tcaatccaga taaagacaat cccgtagacg attaaagcga tggcaatcgg
    10741 aaccataaaa taaaaatgtg cttcaaacca attatcaaga ggaaccgcta ttaaaataga
    10801 aggaatacac gcaatcacca cttttagcca taattgccag gtaagctgta cttctcttgc
    10861 tgttttccca ggttgaaagg gattgagacg ttcaaaataa atcaacataa cagcgataat
    10921 cgcccctaat tgaataacga tattaaacat ttctataaaa gccttatcct gatttagtcg
    10981 gataaattcc tgcactaaaa tgaggtgacc agtgcttgaa actggtaacc attctgtgat
    11041 tccctcaatg atgccaaaga aaatggcttt tagcaattcg ataattaaca tgttttctcc
    11101 ttattaacca ctcttattgt gattttctga actgtgatta atgccaagca gcttttttaa
    11161 aaggcaccgc cgccgcctcc tccgccaccg ccagagaagc cgccaccacc actaatcccg
    11221 cctccggaag agactgagaa atttgaggaa gttgtagctg aagacaagct agacacaaag
    11281 gttggtgtcg tagcatacat taccattgat aattcacctg gtcgaacagc ttggtaaacc
    11341 tcaggtaagg caatacgatg gactttcaaa tagcgttcca ctttcttagc gtaaccaaat
    11401 aaggtggcat aaaccaaaac acggttccaa acaaccaaac cttcaatagc aacatcctca
    11461 aatttgtcaa tgtctcgtat catgttttta aagctctgcc attgatgtaa ccgaacaccc
    11521 ccctcaggtg tttcaatacc caatttcttg tattgatcta acttcaggct aatgtaaaag
    11581 ccaaggcaca tcgttagcac cattaaagca aggtaaatga gggctaaccc actttcttta
    11641 atagccaagt aaatcaataa accactattt agaataagca acaaacaacc taatccctgg
    11701 acactattgc tttttcgttt ctctgccggg gtcatttgac gataagtatc tggtaagttt
    11761 aactttttga tagtttctag gacattgttt gtaatagctg ccgacgcttt tttcatcgcc
    11821 ttaatcacct gctcagacga ttgacggact tcttgctcga gctttttacc tttgtacgtt
    11881 ttctttaatt gtttaatggt atcagcatca taatggtatt gactaaacaa ttgatccacc
    11941 ggcaatgtca ccttgtttcc aaaagccata tctaagaagg cagcttcctc atctgtcaca
    12001 cgatctaact gactgatttc aagataagct ttcccttctt ctttcgttaa taaaagcacc
    12061 ttttggtcga tcagatccaa caaggtagct tgaacaaggg cctcaaaggt cactccttta
    12121 gggatcaata atttctggct ttctgatgct gtaggggata aataggcaaa actctgtcca
    12181 taaatcgctt gtgtcaacac aagaggcgaa agatcttcag ggacttcgta actgtgatct
    12241 gtatggttag ccaaatgata acgattgaat tgtttcttta atcttgtaaa ctgtattaac
    12301 tgcgaaagaa gaaaacctac ctctaccaga ggaatgactt tgccaaatag cagctgtaac
    12361 aaggtccttc gtctggaaat cttagtttct aacgctacaa ttttattctt tttactcgtt
    12421 gaaacatcaa ctggctcttt tcccaataat gcttttttat cccaataagc atgcaattcc
    12481 aattgccctg agacattctt ggcattaatc tgatagcgac tgttaccttc ttttaacact
    12541 tgaggtttct tttggtaata tcctctgtga gcccatagat tagaatcctg aatatcagtt
    12601 ggggtagtga cggtcaaact caccttttct aatgttttgt cccaatcact aatcggtgtc
    12661 caattcagct ctgccacgtc ttgataagct gttaaaaggt ggtgcagttg ccagataacc
    12721 ttgacgtcaa ccttatcacc agcttggcca gcattataaa gttttaaacg atagccatct
    12781 ccaaggtcac taaactcttg actaacaggt acctgttgac cattctgata tacctcaact
    12841 ttaggctttt ggtcaatagc aaagcccgct ggcaaatgac ctatccggcc cagcgaaata
    12901 tactggccgt tataagaagt gtcaaattga taggttactt tttgctcaaa ccgagcagta
    12961 ttttcttttg ataacagtaa ctgaccttca tagttggtga tgctatagtc tacatcagct
    13021 gcttttattc gtatccctaa aagactgaag cagagcacaa gcgtcatcaa aatttttttc
    13081 atggcaacat cctctttctg ccctcatttt accatatttt aaggagatga caaaacctaa
    13141 accaagtcct tcatcttatt tttactcatt attttgaata tttttccata tttcaaccta
    13201 ttcactgata atatattgaa ataggatgca aattgctgta aaataggtct gaatactatt
    13261 tttgaaacag gagacctata tgaaaaagct aatccttagc tgtttggtcg ccttggccct
    13321 tctgtttgga ggaatgagcc gggctcaagc aaaccaatat ttaagagtcg gaatggaagc
    13381 agcctatgct ccttttaact ggactcaaga tgacgcttca aacggggctg tccctattga
    13441 gggaactagc caatacgcca acggttacga tgtccaagtc gctaaaaaaa tcgctaaagc
    13501 tatgaacaaa gaacttttag tcgttaagac ctcttggacc ggtttaattc cagcattaac
    13561 ttctggaaaa atcgatatga tcgctgctgg tatgagtcct accaaagagc gtagaaacga
    13621 aattagcttc tcaaacagct actacactag ccaacctgtt ctagtcgtaa ctgctaatgg
    13681 caaatatgct gaggcaacaa gcctcaagga tttttctgga gctaaagtaa ctgcccagca
    13741 aggcgtttgg catgtcaatc tcttaactca actaaaaggt gctaagttac aaacaccaat
    13801 gggagatttc tctcaaatgc gacaagccct tacttcgggt gctatcgatg cctatatttc
    13861 tgaacggcct gaagccatga ctgctgaagc tgctgatagc cgtttgaaaa tgatcactct
    13921 taaaaaaggg tttgctgttg ctgaatcaga tgctgctatc gctgtcggaa tgaaaaaaaa
    13981 tgacgatcgt atggcaactg tcaaccaagt gcttgaagga ttttctcaaa cggatcgtat
    14041 ggccctgatg gatgatatgg ttaccaaaca acccgtggaa aagaaagccg aagatgctat
    14101 agcatcattt ctaggccaaa tgtgggctat ttttaaaggt aactggaagc aattcttacg
    14161 tggaactgga atgacccttc tgatttccat ggtcggaacc attacaggtc tctttattgg
    14221 attattaatc ggtattttcc gtacagctcc taaagctaag cataaagtag ccgccttggg
    14281 acaaaaactc tttggttggt tactcactat ttatatcgaa atcttccgtg ggacacctat
    14341 gattgttcaa tctatggtta tctactacgg aacagcgcaa gcctttggta tttcgatcga
    14401 ccgtacccta gcggctattt ttatcgtatc tatcaatacg ggtgcctata tgagtgaaat
    14461 tgttcgcggt ggtattttcg ctgtcgacaa aggtcaattc gaagcagcaa ctgctcttgg
    14521 ctttactcac ggacaaacca tgcgtaaaat cgtgctacca caagttgttc gcaacatttt
    14581 accagcaaca ggtaatgagt ttgtcatcaa tatcaaagat acttctgttt tgaatgttat
    14641 ctctgttgtg gaactttact tctcaggtaa taccgtagcc acacaaacct accaatattt
    14701 ccaaaccttt acgattatcg ccattatcta ctttgttctt accttcacgg tgacacgtat
    14761 tcttcgttat attgaacgcc gtttcgatgc cgatacttac accactggag caaaccaaat
    14821 gcagattgcg gaggtctcaa atgtctaact caattattga aatcaaaaac ctcaaaaaat
    14881 cttacggcag caatgaagta ctcaaagata tctccctttc tgttaacaag ggagaagtga
    14941 tttccattat cggttcttct ggttctggaa aatcaaccct cctgcgctcg atcaatcttt
    15001 tggaagagcc aagtgccggt caaatcctct ttcatggtga agatgtcctt gctgaacact
    15061 acaacttgac tcactaccgt gaaaaactag gcatggtttt ccaatccttt aacttgtttg
    15121 aaaacctaaa tgtattggaa aatgctatcg tcgctcaaac caccgtcctc aaaagagacc
    15181 gtgcacaagc cgaacaaatc gctaaagaaa acctcaatgc ggttggtatg acagaacaat
    15241 actggcaagc caaaccaaaa caattatcag gggggcaaaa acaacgggtt gccattgcca
    15301 gagccttatc tgtcaatcca gaagccatgc tctttgatga accaacatca gctcttgatc
    15361 ctgaaatggt tggtgaggtc cttaaaacca tgcaagattt ggcaaaatca gggttgacca
    15421 tgattattgt tacccatgaa atggaatttg ctcgtgatgt ttctgaccgt atcatcttca
    15481 tggataaggg attgattgct gaagaaggaa gtcctcaaca aattttcgag aatcctacac
    15541 aagatcgtac caaagaattt ttacaacgct tcttaaaata agataaagga tccaactatt
    15601 tctatgccag ttgggtcttt tttcaattca aaaagaattt gtcataaaat actttaccag
    15661 ttaactattt tatgctatac ttatttttgt taacgttaat aatttatatt taggaggata
    15721 atatggcaga aaaaactgac ttatcgtcag cttacagaag actgaaaagt cccaatatta
    15781 aaaccagaaa acgtgctctg aagattattc acgagtacaa acgttacggt aaaaaataag
    15841 attttacttt ttatctctcc aaatttaagt cagacaaaaa aaggctaaga caccccttcc
    15901 atcttagcct ttttgtatac agtttattaa gggaactatt ttaaaatatc ttttatgcta
    15961 gagtctagaa tagttcaaaa cgttaaaatc atacatttaa ggtagtgttt aaagtatacg
    16021 aaaaagaact cagcataaac caagctctct ttcgttataa ctaattattt agcaattttt
    16081 gcgaagtact caagagtacg tacaagttga gcagtgtaag acatttcgtt gtcataccat
    16141 gatacaactt taaccaattg tgatccgtca acttccatta ctttagtttg agttgcgtca
    16201 aacaatgaac cgtatgatac gcctacgata tctgaagaaa cgattggatc ttcagtgtaa
    16261 ccgaagctat cgtttgaagc agctttcata gcagagttga tttcgtcaac agaaacgttt
    16321 ttgtcaagag ttacaaccaa ctcagttact gatccagttg gaacaggaac acgttgtgca
    16381 gcaccgtcaa gtttaccgtt aagttctggg ataacaagac cgatagcttt agcagcacca
    16441 gttgagttag gaacgatgtt tgcagcacca gcgcgtgcac gacgaaggtc accaccacgg
    16501 tgtggtccgt caaggatcat ttggtcacca gtgtaagcgt ggattgtagt cataagacct
    16561 ttttgaatac cgaatgcatc gtgaagagct ttagccatag gagctaaaca gtttgtagta
    16621 catgaagcac ctgagataac tgtttcagta ccgtcaagaa tgtcgtggtt agtgttgaaa
    16681 acaactgttt taacatcgtt tccaccagga gctgtgataa caactttttt agcaccgtta
    16741 gcatgtaagt gtttttcagc tgcttctttt ttagcaaaga aaccagttgc ttcaagaacg
    16801 atttcaaccc catcagttgc ccagtcgatg ttttctggat cacgttcagc agaaactttg
    16861 atgaagtttc cgtttacttc aaatccacct tctttaactt caactgttcc atcaaaacga
    16921 ccttgagttg tatcgtattt caacaagtgt gcaagcatat ttggatctgt aaggtcattg
    16981 atacgagtta cttcaacacc ttcgatgttt tgaatacggc ggaatgcaag acgtccgata
    17041 cgaccgaaac cgttaatacc aactttaact accattagtg atttcctcct tatgaaaatc
    17101 aaaagaatat tatttagagg gaatgctccc tacctattgt gaaaagatta acttacagtc
    17161 atctgcaaca acctttcaac agaactatta tataactatt tcagaaaaaa tgcaaataaa
    17221 ttagccattt tcataaaggg cagcttcttt cttctagcct tcaaaaccca ccaaatatag
    17281 gaaagggctt tctttttcta ttctgtaatt tatcttttta ttgggaaaga tttccaaaac
    17341 ttcaaactgt actataacgt tctgttacac taaaaaagag gttaggatag ttatcctcaa
    17401 cctcttagtg gtggttattc acctttgttt ttcttaatga tttcttcttg aactgactta
    17461 ggaacatctt catagtggtc aaataccatc atgaacgtac cacgtccttg agttgcagaa
    17521 cgaagaactg ttgcataacc gaacatttca gcaagtggta cataagcacg aacgatttgg
    17581 ctgttaccat gtgcttccat accatcaaca cgtccacgac gagctgtcac gtgtcccata
    17641 acgtctccaa ggttatcttc tggagctgta atcgttacaa gcatcatcgg ctcaaggata
    17701 gctggttgtg cagatttagc tgcttcttta agtgcaagtg atgctgcgat cttgaaggca
    17761 gtttcagatg agtcgacatc gtggtatgaa ccatcgtaaa gcttagcttt aacatcaacc
    17821 attgggtaac ctgcaagaac accgtttgcc atagattcga tcaacccttt ttcaacagct
    17881 gggataaatt cacgaggaac tacaccacca acgatagcat tttcgaattc gaaaccttta
    17941 ccttcttcat ttggtgtaaa ttcaatccaa acatcaccga attgaccttt accaccagat
    18001 tggcgtttga agaatccacg agcttgtgta gaagcacgga atgtttcacg gtaagatact
    18061 tgaggagcac caacgttagc ttcaactttg aactcacgtt tcatacggtc aacaaggaca
    18121 tcaaggtgaa gttcacccat accagcgata actgtttcac cagtttcaac gtttgtttca
    18181 acgcggaatg ttggatcttc ttcagcaagt ttttgaaggg caacacccat cttgtcttgg
    18241 tctgctttag attttggctc aaccatcaac tggataactg gttctggaac ttcgattgac
    18301 tcaagaataa cttttgcttt ttcatcagtc aatgagtcac cagttgttgt gtctttcaaa
    18361 ccaacagcgg cagcgatgtc accagcgtaa actgtttcaa tttcttgacg gctgtttgcg
    18421 tgcatttgaa ggatacgtcc gatacgctca cgtttacctt ttgatgtgtt cataacgtaa
    18481 gaaccgctat taagaacacc tgagtaaaca cggaagaatg tcaaacgacc tacgaatggg
    18541 tcagtcatga tcttgaaggc aagagctgca aatggctctt catcagatgc tggacgttct
    18601 tcttcagcat ctgtatccgg gttaacacct ttgattgcag ggatgtcaag tgggcttgga
    18661 aggtatgcga taaccgcatc aagcatcaat tgaacacctt tgtttttgaa ggcagaacca
    18721 caaagtactg ggaagaattc aacgttgatt gttgctttac ggataccagc aatcaattcg
    18781 tcgttagtga tttcttcacc ttcaaggtat ttcatcatca agtcttcatc agtttcagca
    18841 actgcttcga ttaatttttc acggtattct tgagcttgtt ctaagtattc ttctggaata
    18901 tcttcttcaa ggatatctgt accaaggtcg ttggtataga tttctgcttt catcttgatc
    18961 aagtcaatga taccacggaa atcatcttct gcaccgattg gcaattggat tgggtgtgca
    19021 tttgcttgaa gacggtcatg aagggtttgt actgagtaaa ggaagtcagc accgatttta
    19081 tccattttgt tggcgaaaac gatacgtgga acaccgtatt cagttgcttg acgccaaact
    19141 gtttcagttt gaggctcaac acctgattgt gagtcaagaa cggttacagc accatcaaga
    19201 acacggagag aacgttgtac ttcgatggtg aagtccacgt gtcctggtgt gtcgatgatg
    19261 ttaacgcggt gaccatccca ttgagctgtt gtagcggcag aagtaatagt gataccacgt
    19321 tcttgctctt gctccatcca gtccatttgt gatgcgcctt cgtgtgtttc accgatttta
    19381 tgaattttac cagtgtagta aaggatacgc tctgttgtag ttgttttacc agcatcgacg
    19441 tgagccatga taccgatatt acgagttttt gcaagtgaaa attcgcgagc catgtgtttc
    19501 tcctattttt ttagtttact ctttcattat agcactattt agcaaaaacg gataggcagg
    19561 acctacccgt tatctaaata tttgttggta aagataatac ctattgcctt tataaggctt
    19621 ggtatcttat tcattgaact caggctaaac gctgtgtgaa aaagataaac tttcctagag
    19681 acaaaggttt cttcgtcaaa tttcctattt tcactttgcg ttctatacgc cttcgtatct
    19741 tattaattga gttcgagcta caagccactg acaaaaagat aatagttcct agaaactgaa
    19801 gtttctgcgt cactattcct attttgtctt ggacttgctt aacactctca catcttatat
    19861 tagttgaatt ttagctacaa atgtctgcca caaagataaa attacctaga aactgaagtt
    19921 tctgcgtcaa tttccctatt ttggctagca tttgtttgac gctaaaatat cttatattac
    19981 caacggaagt gagcaaaggc acggttagct tcagccattt tgtgagtgtc ttcacgtttc
    20041 ttaactgatg cacctgtgtt gtttgcagca tccatgattt ctttagcaag acgatctttc
    20101 atagtgtgtt caccacgagc tcgtgatgcg tttaccaacc aacgaagtcc aagtgttgta
    20161 cgacgttctg gacgaacttc aactgggact tggtagttag aaccaccgac acggcgtgcg
    20221 cgtacttcaa gtacaggcat gatgttgtcc atagctgttt caaatacttc aagagcgtca
    20281 tttcctgttg cttctttgat agcgctgaaa gcatcgtaaa cgatagtagc agctgtacca
    20341 cgtttaccgt caagcataac acggttgata agacgtgtta cgatttttga gttatataat
    20401 ggatctggta atacttcgcg tttaggcgct tgatttttac gactcatttt ctcttatccc
    20461 ccttctatta tccttttgga cgtttcgcac cgtatttaga acggccttgt ttacggtcag
    20521 caacacctgc agtatcaagt gcgccacgaa cgatatggta acgtacccct ggaaggtctt
    20581 ttacacgtcc gccacgaata agaacaacac tgtgctcttg caagttgtgt ccgatacctg
    20641 ggatgtaagc tgtaacttca ataaggttgc tcaaacgtac acgagcgaat ttacgaaggg
    20701 ctgagttagg ttttttaggt gtcattgttc caacacgagt agcaacgcca cgtttttgtg
    20761 gggcagccat tttagtttga acttttttgt gactgttgta accaatgttc aaagctggtg
    20821 aatctgattt ttcaatttta gatttacgtg gtttacgtac caactggtta attgtaggca
    20881 tctacattct cctgtgtttt tttatttttg gttgatatag cacttggtga cagcccatat
    20941 ccggtgcact ttttgcaaca attgtcagca cgtctctgta cacttttgag agaccaaaag
    21001 taaaaagtac cgtctaatat agtaacacag acggcacttc atgtcaaata gaatttttcc
    21061 tttgatgcaa ttgcttatga cgaatgcatc acctagattc cttacggaac ctaaatcctg
    21121 tgaggccgac agctgccaac atgacgactc ctgctttaag agctctttga gtactttcat
    21181 caacatcaga aatgagattg ctcgttgtag aagatcctga gccataagat gtcttagatg
    21241 agggctgagt aatcgaagat gtgattttag acacaatggc attacttgct accatttcag
    21301 aggtttggcc aacaagcgct tctgctacaa gacttgtatt ttcagcagct aatcttgctg
    21361 aagcttctaa tagttgtttc gtttctttaa ccatttcttg aacaagcgga gtagtctcta
    21421 ccttactata tgatagcggt tttacgcccg taagagatgg agctacttgc aaatcaggca
    21481 cagtagctat atcttcgtct aagtggcgat attccttttc attagctaag gttttaagca
    21541 aagtcaactg gtgttctgtg gtagcaatag tagcttctag actgctttgt ttagcttgta
    21601 aggctgctaa agcttcttgt gcatttaaca aagatgaggt agttttagcc aaatcttgct
    21661 tagtattatc aatgcgctca cgtatcactt gaaggcggtt aggattcaat ttaaagtccc
    21721 ttaagtattg caaatgagct tttttagcca ccagtgctgt cactctggct gcggcttgct
    21781 ctgctaaggc ttctgtctgg tgcagtgcgg ctttcaacga tgctagctta gccaatgatt
    21841 gatcacgagt agcttcgagt tgtgcttgtt ttgctttagc tgctagtaat tctgttctca
    21901 aagaaccttt agtatcattt aattgtctca cttggaggtt taagctgtct gactgcttaa
    21961 gtgtgcttgc taatttacct tgaagttgac ttactttagc ttgggctgcc ataatatcag
    22021 cttcttgatg aatagccgac aaacgatttt ctaatgagct tacttttcct ttgatcgctg
    22081 caatagtatc agatacagtg cctactcttt gggcataatc ttttgtactt ccaacggctt
    22141 ttataggggt cttattaaag cgttgatggt tagcaatgtt agactctgga aacattacaa
    22201 agtgttcatt caaagatcct acattgctgg ttgaaaatcc aaggtaaaca ggcgcattag
    22261 ggttacgttt atctacacgt aaaaagttaa tagcatggcc gtatgtattt ccgtgtaaat
    22321 gatctgtaaa gagcatatac ttgatactgt cataaatacc acgtttaata ccattcacag
    22381 tatgcacatc gttaaaagca ccgatattct cgtacatgtt atcatcattt cgaatgagcc
    22441 ctgacgctcc ggcagagtct tcaataatag ttttatcatg aggcccaaca ccataatgcc
    22501 ctgatacccc tggctgtccg tagacaaatg atggtcttgt attaccatga gttttcttat
    22561 agctggtact aagtaatctt gcaaattctt gtgatcctgc tgtaacagta actggtggta
    22621 gacctaattg tcttcttaca ctattaatca tgtgagctgc aaactgcgct agctcatttt
    22681 gcacttctgg tgtcaaatta tcgggatcaa caaagcgatt acgatctgct ggaatatctt
    22741 ggtattgatt taattgatta cctggactag ctttggcaat aatttgatct gcatgctctt
    22801 tgtaataatt attgtaacta gctgatccaa tataaccact agcttctaat tttttaagtt
    22861 cttcaagagg atagccttgc ggtgctttca tggtattatt acccacaatg ctatcttgag
    22921 tagacggagc tgaggattta agacgactaa gttctgcctc tttttctgct agagcagctt
    22981 tctgagctgc caactcttca gtcaattgct ttttaacttt agctttttga ttttctaagt
    23041 cagctttagc cttctccaat tctgagctta atgcttttgt attatcatta gccgtttgag
    23101 ctgctttagt gatagcatca ggattgctaa tcatagcatt gagcttagca atattttgtt
    23161 cagacgtttt gacttgttcc actaaatctt gagctcgagt agtttctgct gaaatgctag
    23221 ctttttgttc tgacaatgca gtctcttttg aatgttgatc tgcttgagca ttatgaagct
    23281 ctgtttcagt agctgttaac tctctttgat gttcggctcc ttgggctaat agcgtctcct
    23341 cactacttgc aagagtatta gtgtaaattt cttgtgcaga ggttaaagct ttttgttcat
    23401 tatcttgctg ctcttttaag ttgttgattt cagcagtagt ttttgtcaga gcggtagcaa
    23461 gctctgtcag ttctgctttt tgttgactga gagttttttc aactgcatca atagttgcct
    23521 ttgcctcttg aattgtttct ggttttggta aactatcgtc gtgagtatta ctcgccttcg
    23581 tttctcctga ggctctatca tctgctttga cttggtgaga tacgcctaca ctggcactca
    23641 ataaagcaat tgttgaggtt aaagcaattt tctgcttaac ttggtttggc ttcgtttgtt
    23701 ctaagtccat tctctctcct ttttccattt atcgaaaata ttaccaactt ctacaacaac
    23761 attgttgttt ttgacatcaa tctctgaaac tttcagcaat gatttgaagg tcatttgctc
    23821 acgctctgat tgagcgtttt ctgcaaacac agcaacacca actaaagtgc tgtcaaattc
    23881 tgtcaagaga ctgatcattc ctgtaatcgt ccccccgccc ttcaagaaat catctacaat
    23941 caatacacga ctattgggtt tcaaactacg ctttgacaga aacatctttt caatacgatc
    24001 actagaagca ctcgcataat tgacagacac tgtcgatcct tctgttattt ttaaatcacg
    24061 tctaacaatg acaaaaggaa cacttaaaat attagccact gcatttgcta aaggaactcc
    24121 tttggttgca actgtcatca cagcgtcaat cttttctcct ttaaaggcat tggcaataat
    24181 acgaccaata ttttgtaaaa ttttaggtgt actcagcaag tctgacaaat agatatagcc
    24241 tccaggtaag atgcgatcac tctctgataa cctttgacac aggtcttcaa cgatggtacg
    24301 cgcttctgtt tctgaaatac tgggggtaaa aattacgcct ccacttgctc ctgtaagtgt
    24361 atcgatatca ccaatgttgg cttcttcaaa agctttcttg ataatagcga tatcttctga
    24421 gatagaagat tttgcggcct catatttagt tgcaaaggtg tttaaactag ttaatttata
    24481 cggattgtta atcaaataat ttgaaatgac aaccatacgt tcacttcgtc ttaatttcat
    24541 agtttcccca tttttatgta ctttttgct
//
LOCUS       DAUGFP010000024        21994 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.024, whole genome shotgun sequence.
ACCESSION   DAUGFP010000024 DAUGFP010000000
VERSION     DAUGFP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21994
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.024"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            79..333
                     /locus_tag="VH663_000986"
     CDS             79..333
                     /locus_tag="VH663_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEQ8174197.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            351..746
                     /locus_tag="VH663_000987"
     CDS             351..746
                     /locus_tag="VH663_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEQ8174198.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            1031..1350
                     /locus_tag="VH663_000988"
                     /pseudo
     CDS             1031..1350
                     /locus_tag="VH663_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264372.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1347..1910
                     /locus_tag="VH663_000989"
     CDS             1347..1910
                     /locus_tag="VH663_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ8174199.1"
                     /translation="MNLIIIGAQASGKMTIGQEVARQTGMTLFHNHDSIDFVLRFMPW
                     SQESTALIERIRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTQLTAAETAQLIIQKMTHIKEN"
     gene            1912..4560
                     /locus_tag="VH663_000990"
     CDS             1912..4560
                     /locus_tag="VH663_000990"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEQ8174200.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKCVHSVGHSERSGAAVEPRLSTQWFVKMDELAKQAMDNQETDDRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIL
                     RLLHPIMPFVTEEIYAQYAQGSIVTAVYPTVTPTFENEAAHKGVESLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLGNERFVANAKPEVVQKE
                     KDKQADYQAKYDATQERIAEMKKIKS"
     gene            4714..7122
                     /locus_tag="VH663_000991"
     CDS             4714..7122
                     /locus_tag="VH663_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HEQ8174201.1"
                     /translation="MRMILAHYDCKKDKKQSLDEHLWHVACSSRQEASIIGQGDVLFL
                     IGLYHDLGKADRTFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKNRGVDKNERMTFN
                     EMVGYVISAHHGMYDLCYYFDDAEYYGFNKFKNRINRDLDGYHYHEDIKGYALKLEKK
                     LCDYGYKDLRELIDKAFDNYQQAMSSLNWQDKSEWNYYQSCMVRLYLSLLKNADILDT
                     VNAYGLKISPMDKTERSFLKHSYLAAIEQKYASFGQPNNQLNTIRTEIAERVKERGKR
                     DSKGIYRLDLPTGAGKTNLSMRYAFHQLVHHDKSRFFYITPFLSVLEQNASEIRKVTG
                     DLGVLEHHSNVVKQANEDDDDKDSLLSAYLSDSWDSQVVLTSMVQFFQTLFKTKSANL
                     RRFSSLINSIVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYDSSEID
                     HRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVHLTDVINLILGEENSVL
                     AIFNTKKTVHNCYTMLKDMTDRPVYQLSTNMCAQHRLDLIAKIKTELQNNIPIICIST
                     QLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEENISRLT
                     EIKAKKEATESILHKIGSPIDISTLNRDFFEYYYANNQGLMDYPLEDNLSIYDYLSLN
                     IYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGEAEKKLAYLEELGMSHFLS
                     AEDYQTIKSLLKELQPFTVNVRENDPLFETTKSYLNGQILVLTSEYYDTERGVKYDSA
                     SFYF"
     gene            7255..7983
                     /gene="cas5c"
                     /locus_tag="VH663_000992"
     CDS             7255..7983
                     /gene="cas5c"
                     /locus_tag="VH663_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEQ8174202.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            7983..9878
                     /gene="cas8c"
                     /locus_tag="VH663_000993"
     CDS             7983..9878
                     /gene="cas8c"
                     /locus_tag="VH663_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEQ8174203.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRSNESVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QTQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANREKLGI
                     CNISGREEQLTDKHRGLMGNTKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYHKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            9883..10731
                     /gene="cas7c"
                     /locus_tag="VH663_000994"
     CDS             9883..10731
                     /gene="cas7c"
                     /locus_tag="VH663_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEQ8174204.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKPIFVQANERIEDDFRSLERRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYHQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     EILEGL"
     gene            10733..11407
                     /gene="cas4"
                     /locus_tag="VH663_000995"
     CDS             10733..11407
                     /gene="cas4"
                     /locus_tag="VH663_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEQ8174205.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQI
                     LHTKADNPYIKEKRKDLLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMHEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            11404..12429
                     /gene="cas1c"
                     /locus_tag="VH663_000996"
     CDS             11404..12429
                     /gene="cas1c"
                     /locus_tag="VH663_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ8174206.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGRASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILMTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            12440..12733
                     /gene="cas2"
                     /locus_tag="VH663_000997"
     CDS             12440..12733
                     /gene="cas2"
                     /locus_tag="VH663_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ8174207.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVNYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     repeat_region   12882..13445
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12882..12913
                     /rpt_unit_seq="gtctcacccttcatgggtgagtggattgaaat"
     gene            13604..14314
                     /gene="ccdA2"
                     /locus_tag="VH663_000998"
     CDS             13604..14314
                     /gene="ccdA2"
                     /locus_tag="VH663_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEQ8174208.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNIFYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHILLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            14327..14950
                     /locus_tag="VH663_000999"
     CDS             14327..14950
                     /locus_tag="VH663_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEQ8174209.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMAKKEITQDKMSMAAKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFKKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            14993..16099
                     /gene="msrB"
                     /locus_tag="VH663_001000"
     CDS             14993..16099
                     /gene="msrB"
                     /locus_tag="VH663_001000"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ8174210.1"
                     /translation="MEHKWKLWLFIVGVVFLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKVDPNAPVDQKVIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVTYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEDYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSAAEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVIRYKEDKSFNMTRTEVRSRSGDSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            16187..16927
                     /locus_tag="VH663_001001"
     CDS             16187..16927
                     /locus_tag="VH663_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ8174211.1"
                     /translation="MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLRKKLELSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKELGMPFQDYLIQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLIGVTPSQYKKGDQA"
     gene            16924..18657
                     /locus_tag="VH663_001002"
     CDS             16924..18657
                     /locus_tag="VH663_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8174212.1"
                     /translation="MKRYPLLVQLISYIFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNTEIQAFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVSTNSQLIMKTSSDMMAEPWYKAAIDRQAMPILTPARQLSLSS
                     KKEWVVSVTQEVVDAAGHNLGVLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIKNGYAKDKSSFVYQRVIPNSQWTLIGVASLDQLHRV
                     QRQIFWSFLGMGLLACLICGLTTILVLRKWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTDLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYD
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLMLTVWDNGK
                     GIEDSSLTNSQSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDHFTEIQLTLPKMH
                     ELMADDTQENE"
     gene            18730..19809
                     /locus_tag="VH663_001003"
     CDS             18730..19809
                     /locus_tag="VH663_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEQ8174213.1"
                     /translation="MSLASDYFSRQTPIVEKLVAYGFEKRDNGYFYNERFMEGEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLGRLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVGGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKAFPQLLQQEGIYPAYHMSKKNWISIILDDKVTDDKLWTLVTQ
                     SRQLVNPNGLSNPNGPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYVCQVLETNIPNEGYRKNPNIDKLMRLRKRQQYKDGLLSFDLMKEHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            19823..20539
                     /locus_tag="VH663_001004"
     CDS             19823..20539
                     /locus_tag="VH663_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEQ8174214.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     ITSVHVLDKDNPHVLLFTEN"
     gene            complement(20705..21178)
                     /locus_tag="VH663_001005"
     CDS             complement(20705..21178)
                     /locus_tag="VH663_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ8174215.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATICGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            21320..>21994
                     /locus_tag="VH663_001006"
     CDS             21320..>21994
                     /locus_tag="VH663_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ8174216.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     DYFLQYT"
BASE COUNT         6894 a         3933 c         4766 g         6401 t
ORIGIN      
        1 cagttattaa taacatggca caagaggcct gccttatggt ataatggtaa agataatttc
       61 agaaagagga agccaagcat gtcttatgaa aaagaatttt taaaagattt tgaagactgg
      121 gttaaaactc agattcaagt caatcagcta gccatggcaa ccagtcaaga agttgcccaa
      181 gaagatggcg atgaaagggc taaagatgcc tttattcgtt atgaaagtaa gttagacgcc
      241 tacgaatttt tactaggtaa atttgacaat tataaaaatg gtaaagcctt tcacgatatt
      301 ccagatgagt tatttggagc aaggcattat taagaaggag acacgaaata gtggcaaaac
      361 aaaaaaatcg taaaaaagca ttgaaattag aaatgaagcg taatggcttg ttaaaaaaag
      421 caggtcaagt ttttgataag gcagttgaaa gtgttgaaac agctgtggat aaaaccattt
      481 ctgctggtaa aaatcttgtg gaaaaaggca gccaaacggt tgaaaacctg acagcaagca
      541 aggaaagact tactcttgat gagtttgcag ggcagccagt tgtagctggt atccgctctg
      601 accttgtcga aaccctatat gcagaaggca ttcattcagc gcaagctttt aaggagtgga
      661 cagaaaaaga cctccttgct cttaagggca ttggcccagc aacggttaaa aaattggtcg
      721 aaaatggcgc aagctttaaa aaatagtcat cttctcgttg cttaatggct ataaatttgt
      781 taaaatatta gcaatcagag gtgcattgag ttggtctgtt acagagagcg gtggtgttga
      841 gaaatccgca cagatgtcaa ggctagtagc tgaggatcag aaatcagata tgaaaagtag
      901 cagacccatt ttatggtttg aaacagtggt ggtaccgcgg aaatcgtaac acgaaattcg
      961 tccctgtcta ggttatctag gcagggatta tttggtttca tttgaaagaa gatgatggct
     1021 tattgttaaa atgttgttag aagtatataa tagaaaaact ttgcccaaag tggaaacaga
     1081 acggctgcta ttacgtcaac gaaaagggga caaggcattg ttccagaagc ggcttcagct
     1141 ctgattgaaa ttggttttac cttacttgat ttgcataaaa ttgagctggg ttgctacgac
     1201 tacaacaagc aaagtcaggc agtggctcgc aaattaggct ttaccttaga agccaatgcc
     1261 cgtgaccgta aggacgttca aggtcgccgc tgtggcgaca tgcgttttgg gttactgaga
     1321 agtgagtggg aggagcagaa acaaaaatga accttatcat cattggtgct caagcgtcgg
     1381 gcaagatgac cattgggcaa gaagttgcca ggcagacagg catgactttg tttcataatc
     1441 acgattctat tgactttgtt ttacggttta tgccttggtc tcaagagagc actgccttga
     1501 ttgaacgtat ccgttttgct ttttttgaaa cctttgctaa gacaggtcaa gacatgattt
     1561 ttactattgt aattgacttc aatgatccaa atgatgtagc aatgcttgaa aagattcagg
     1621 ctgtttttca gtcttatgac caagaggttc tctttgtcga attaaaaaca gatatagagg
     1681 aacgtcttaa acgcaatcgc acagaaaatc gtctcaaaca taaacctctt aaacgcaaca
     1741 tcgagtggtc agaacaagat attcaatcca cgatggcata tgctgttttt aatccagaag
     1801 aaccacccaa aacgctcacg cattaccaaa aaatcaataa tacccagctc accgctgctg
     1861 aaacagcgca gcttattatc caaaaaatga ctcatatcaa ggagaactaa gatgacagaa
     1921 ctatcaccta aatacaatcc tgccgaggtt gaggcaggtc gctatcaaaa atggcttgat
     1981 gcagatgttt tcaagccatc aggagaccaa aaagcaaaac cgtattctat cgtgattcca
     2041 ccgccaaatg tgactggtaa gttgcacttg ggacatgctt gggacacaac gcttcaagat
     2101 attatcattc gtcaaaaacg catgcagggc tttgatacgc tttggttgcc agggatggac
     2161 catgcaggga ttgcgacaca ggctaaagta gaggagcgtc tgcgcgagca ggggatttct
     2221 cgttacgacc ttggtcgtga caaattccta gacaaggtct gggagtggaa agacgaatat
     2281 gccactacga ttaaagagca gtggggcaaa atggggcttt ctgtggacta cagccgtgag
     2341 cgcttcaccc ttgatgaggg cttgtcaaaa gcagttcgca aggtttttgt tgacctctac
     2401 aaaaaaggct ggatttaccg tggtgaattc atcatcaact gggatcctgc tgccagaact
     2461 gccctttctg acatcgaggt tatccacaag gatgttgagg gagctttcta tcacatgaac
     2521 tatatgttgg aagatggctc acgcgccctt caagtggcaa caacgcgacc agaaaccatg
     2581 tttggagacg ttgccgttgc cgtaaatcca gaagatcctc gttacaaaga tttgattggc
     2641 aaaaatgtta ttttaccaat cgtgaataaa ttgattccaa tcgtgggtga cgagcatgcc
     2701 gatcctgaat ttgggactgg agttgtgaaa atcacgcctg cccatgatcc taacgacttc
     2761 gaggttggtc agcgtcataa cttaccacaa gtcaatgtca tgaacgatga cggtaccatg
     2821 aatgagttag ctggcgactt tgcaggcatg gaccgttttg aagctcgtca agctaccgtt
     2881 gctaaacttg aagaacttgg cgccctagta aacatcgaaa aatgtgttca ctcagtaggt
     2941 cactcagaac gttcaggtgc agccgttgag cctcgtctat ccacccaatg gtttgtaaaa
     3001 atggatgagc ttgctaaaca agctatggac aaccaagaaa cagatgaccg agtagatttc
     3061 tacccaccac gctttaatga taccttcctt caatggatgg aaaatgtcca tgactgggta
     3121 atctcgcgtc agctttggtg ggggcaccaa attcctgctt ggtacaatgc agaaggtgag
     3181 atttacgttg gcgaagaagc accagaaggt gatggctgga ctcaagatga agatgttctt
     3241 gacacttggt tctcatctgc tttatggccg ttctcaacta tggggtggcc tgacacagat
     3301 gtagaagatt tcaaacgtta tttcccaacc tcaaccttag taacaggtta tgacatcatt
     3361 ttcttctggg tgtcacgtat gattttccaa tcgcttgaat ttaccgggcg tcagccattc
     3421 caaaatgtcc tgattcacgg tcttattcgt gacgaagaag gacgcaaaat gtctaaatca
     3481 ttggggaacg ggattgatcc tatggatgtc attgagaagt acggtgccga tagcttacgt
     3541 tggttcctct caaacggctc tgccccagga caagacgtgc gtttctcata cgaaaaaatg
     3601 gacgcctctt ggaatttcat taataaaatt tggaacattt ctcgctatat cctcatgaat
     3661 aatgaagggt tgaccttgga agaagctgaa agtaatgtgg ctaaggtagc tgcctcagaa
     3721 gctggaaatg tgactgacca gtggattctc cacaatctca atgaaaccat cgctaaagtg
     3781 acagaaaact ttgacaagtt tgagtttggt gtggcaggac acattcttta caactttatt
     3841 tgggaagaat ttgccaactg gtatgtggaa ttgaccaagg aagtgctgta tagcgacaac
     3901 gaagctgaga aagttatcac gcgctctgtc cttctttata ctttggacaa aatcttgcgt
     3961 ctccttcacc caatcatgcc atttgtaacc gaagaaatct atgcccaata tgcccaaggt
     4021 tctattgtga ccgcagtata ccctacggtt actcctactt ttgaaaatga ggctgcgcat
     4081 aaaggtgtgg aaagtctgaa agatcttatc cgtgctgtac gaaatgcgcg cgctgaagtt
     4141 aacgtggcac catcaaaacc aatcaccatt ttggtgaaga cagcagatag tgagttggaa
     4201 gacttcttca ccagcaacgt gaactacatc aaacgtttca ccaatcctga aaaattagaa
     4261 atcagctcag ccattgctgc gccagagctg gctatgacca gcatcatcac aggtgctgaa
     4321 atctacttgc cactagcaga tttgcttaac gtggaagaag aactcgctcg cctagataaa
     4381 gaactggcta aatggcaaaa agaattggac atggtcggca aaaaactcgg aaacgaacgc
     4441 tttgtagcca acgccaaacc agaagtcgtc caaaaagaaa aagacaaaca agccgactac
     4501 caagccaaat acgacgcaac ccaagaacgg attgcagaga tgaagaaaat taaatcatag
     4561 tgtttagaac gcagttgaaa aactgtgttc ttttcttact aatagagata ttagattgaa
     4621 gtttaattat gagaaaaacg ctttctttat ttaaagagtt atgttacaat actattacaa
     4681 tcatcgtaca aatagtgtta ttttgagggg catatgagaa tgattttagc acactatgac
     4741 tgtaaaaaag ataaaaagca atctttagat gagcatttat ggcatgtggc ctgttctagt
     4801 cgacaggaag catctataat tggtcaagga gatgtgcttt ttttaattgg tctttaccac
     4861 gacctgggca aagctgatcg aacctttcaa gataaattat taaataatcc aaatcggcat
     4921 gttgatcact cttatgcagg ggcaaaatac ttatgttcta ttattgggcc tcatctaaaa
     4981 aaccgagggg ttgataaaaa tgagagaatg acattcaacg aaatggtggg gtatgtcatc
     5041 tctgctcatc atgggatgta tgatttatgc tactattttg acgatgctga atattatggc
     5101 tttaataagt ttaaaaatcg tatcaataga gacttagatg gttatcacta tcatgaagat
     5161 attaaagggt acgctctaaa attagaaaaa aaattatgtg attatggcta caaagattta
     5221 agggagctta ttgataaagc ttttgataat taccaacaag ccatgtcttc cttaaactgg
     5281 caagataaga gtgagtggaa ttattatcag tcttgtatgg tgagacttta cttgtcactc
     5341 ttaaaaaacg ctgatatttt ggacacagta aatgcctatg gccttaagat aagtcctatg
     5401 gataaaacag agcgatcctt tctaaaacac tcctatttag cggccattga acaaaaatat
     5461 gctagctttg gacagccaaa caatcagttg aacactattc ggacagaaat cgctgagcgt
     5521 gttaaagaaa gaggtaaacg agattccaag gggatttatc gcttagattt accgacagga
     5581 gctggcaaga ctaatcttag tatgcgttat gcgtttcacc aattagttca tcacgacaaa
     5641 tcaaggtttt tttacataac gccctttctt tcggttcttg agcaaaatgc ttccgaaatt
     5701 agaaaagtta caggtgacct tggcgttcta gaacaccatt ccaatgtggt gaaacaggct
     5761 aatgaagatg atgatgacaa ggacagttta ttgtcagctt atcttagtga tagctgggac
     5821 agtcaagtag tcttgacttc tatggttcaa tttttccaaa cacttttcaa aacaaaatca
     5881 gctaatctga gacgtttttc aagtttgatt aatagtattg tgattctaga tgaagttcaa
     5941 tccctgccta ttgaagtcac cactttgttt aatttaacga tgaatttttt aaataaagtt
     6001 atggatacaa ccatcgttct ttgcacagcg acacaacctg cttatgattc ttcagagatt
     6061 gaccatcgta tctgttatgg agggaacttg ggagaattag ctgaaatcgt tgagttaacg
     6121 attgaagaaa aacagatttt ttcaaggaca gagcttagaa aatttgatga tagtgatcag
     6181 aaagttcact tgactgatgt tattaacctt attctaggtg aggaaaactc cgttcttgct
     6241 atttttaata cgaaaaaaac ggttcataac tgctatacta tgctaaaaga catgactgat
     6301 agaccggtct atcagctttc gacaaatatg tgtgcgcagc atagacttga cttgattgct
     6361 aagatcaaaa cggagttaca aaataatatc cctattattt gtattagcac gcaattaatt
     6421 gaagcaggtg tagatgttga ttttcatcgc gtcattcgtt cctactcagg gattgattct
     6481 attgttcagg ctgctggacg gtgtaaccga gaaggcaaac gagataaagg gcaagtcact
     6541 cttgtcaatc tgaccaatga agaggaaaat atttctaggc tgacagaaat aaaagctaaa
     6601 aaagaagcca cagaatctat tcttcataag attgggtctc caattgatat ctcaacttta
     6661 aaccgtgact tttttgagta ttattatgcc aataatcagg gactgatgga ttatcctttg
     6721 gaagacaacc tatcaatcta cgactattta agccttaata tttatcagac ggcaaataaa
     6781 aagttcaaag gtaagttaaa acaagctttt aaaacagcag gagccaaaat gaacctcatc
     6841 aataatgata tgataggaat tctcgtacct tatggcgaag ctgagaaaaa attggcttat
     6901 ttagaagaat tgggtatgtc tcacttttta tcagcagaag attatcaaac gataaaatca
     6961 ttactaaaag agttacaacc ttttacggtt aatgtccgcg agaacgatcc tctctttgag
     7021 acaacaaaat cttatctaaa tggtcagatt ctggttttga cgtcggagta ttatgacacg
     7081 gaaagaggag ttaaatacga ttcagctagc ttttacttct aactcaaaac gaaagcagat
     7141 taacaaaagg ttgttagagg accttgttaa cctgccaatc atcattaata attattatca
     7201 atttagacta tttaataaaa ttagattaca aaaaaacaga aggaggaaag tagcttgtac
     7261 agatctagag acttctacgt gagagtaagt ggtcagcgag ctctttttac aaatccagcc
     7321 acaaaagggg gatcggaacg ctcatcctat tcggttccga ctagacaggc actgaatggt
     7381 atcgttgatg ccatctatta taagccgacc tttactaata tcgtcacaga ggttaaggtt
     7441 attaaccaga ttcaaaccga attacagggt gtcagggctc tgttgcatga ttatagtgca
     7501 gatttaagtt atgtatccta tttgagtgat gttgtttatc tgatcaagtt tcattttgtt
     7561 tggaatgaag atagaaaaga tttgaactca gatagacttc cagctaaaca tgaagccatt
     7621 atggagcgtt ctattcgtaa agggggacgt cgagatgtgt ttttgggtac aagagaatgt
     7681 ttagggcttg tagatgatat cagccaagaa gagtatgaga ctactgtgtc gtattataat
     7741 ggtgtcaata tcgacttggg aatcatgttc cattcctttg cctatccgaa ggacaaaaag
     7801 acaccattaa aatcatactt tacaaagact gtgatgaaaa atggagtcat tacgtttaaa
     7861 gcacagtctg aatgcgatat tgttaacacg ctttctagtt atgcttttaa agcaccagag
     7921 gagataaaat cggttaacga tgaatgcatg gagtatgatg ccatggagaa aggagaaaac
     7981 tgatggattt ttttacttct ctcttgaaga cttatgaaaa agcagagcta gcagacttgg
     8041 ttgatcacca aaaaagaagt aatgagtcgg ttttactgcc gatttatcat acgagtttaa
     8101 agtcaaatgg caaaaatatc atttcagtga aacttgacaa agatggccag tttcacaagg
     8161 cagaatttat ggcagataag caaatgatta tttttcctgt aacggctgat tctgttgcta
     8221 ggtcaggtag tcatcctgca ccgcatcccc tagtcgataa atttgcttat tatagtgctg
     8281 aaatggggca gactcagtat gattcttttc ataagcaact taataactgg attgattatt
     8341 gtgaggaggg tgacgtcaag aggtttttaa ccattgttca gcaatttatt ttgaaaccag
     8401 aatttctgac actgattctc gattctttaa ttggtcctga ttatcaaaat aatcaattaa
     8461 aagtcacatt ttgtgatgcc actggaaaag aaaaattaat tgatttatca gcttgctttt
     8521 tagaattttc aattgatcag ttccagggct ttaaaaatga atcggtttcg acatttaaaa
     8581 ctttacacca atcctatatt tcttttgttg aagccaatcg tgaaaagctt ggtatttgta
     8641 atattagtgg acgagaggaa cagctcaccg ataagcatag aggtttgatg gggaatacta
     8701 aaatcatctc tgttagtaat aaaagagaag cttataaagg acgttttaga gaacgcgaag
     8761 acgtttttag tgttggctat gaaacttccg aaaagattca tttaatgctc aagtaccttt
     8821 tagaaaataa aaataccagt acttggttag ggtcttctca atatttaatc aactggttta
     8881 gtgatgattt aacaaatgat agtcggttgg atatcgtatc accaatcttt gatgacggac
     8941 ttgaagaaga tgatgatgac gatacgcctc ctgttataac attagcaact gaagacaata
     9001 aaagaattgg taaatcattc atcaagggac aaaaattatt tgctaatgat gccacttact
     9061 acgttgctat tttgaataaa accagcaatg ggcggattgc tttaaaatat tttcgtcagc
     9121 ttcaagcgtc ccaattactc accaatctta acaagtggca ggaaacatac agttgggagt
     9181 cgcgatctaa gtttggaaaa agtcgcttaa gaacccctac ttttcatgac atccttaatg
     9241 tgtcctacgg ggttgatagg gatcgcttcc ttgaattaga taatgataac ttcaaaagtg
     9301 atcaaattca aaagttagtg gcaagtttga ttgatggtaa accgatgcca cagtccattg
     9361 tcaaaaagtt aggtaacaat gttaaagaac gacatcgtta ccataagcac tggtatcaag
     9421 ttgagcaggt ctgcttagca attttacaca aacaaaatgg ggaggaattt tcaccgatgc
     9481 tagatcatac caatcaaaat cgttcctatc tttttggacg attattagca atttttgaat
     9541 taatcgagac cttgcgttat ggcttggatg gaaacaataa cgaccgtatt accaatgctg
     9601 aacgttattg gacagcctat actggacaac caacaaaatt gatgatgtta ttggaaaata
     9661 aaattaagcc ttacgaagaa ccattgaaat taaatcgtcg tggcagttgg atgaaattag
     9721 aaaaagaaaa agaagagatt ttagaactgt taaatcctct gttagagaca gaaacaatgg
     9781 aaaaaccctt agattaccgc tttatttttg ggtattatgc tgagaaaaac tattactata
     9841 caaaacaaaa cactgaagta acagaaagtg aggagtaaaa agatgttgga acacaaaatt
     9901 gattttatgg taactcttga agtgaaagaa gcaaatgcaa atggtgatcc cttaaatgga
     9961 aatatgcctc gtacagatgc caaaggatat ggtgtgatga gtgatgtctc tatcaaacgt
    10021 aagattcgta atcgtctaca agatatgggg aaacctattt ttgtgcaagc taatgagcgt
    10081 attgaagatg actttcgttc actagaaaga cgcttttcgc aacattttac agctaagaca
    10141 cctgacaaag aaattgaaga aaaagcaaat gcattatggt ttgatgttcg tgcttttgga
    10201 caagttttta cttatctgaa aaaatcaatt ggggtgcgtg gaccagtttc catcagtatg
    10261 gctaagtcct tggagccaat tgtcatttcc agccttcaaa ttacgcgtag taccaatggt
    10321 atggaagcta agaataatag tggccgctct tctgatacga tggggacaaa acattttgta
    10381 gattatggtg tgtatgtact taaaggttct atcaatgctt attttgctga aaagactggt
    10441 ttttctcagg aagatgctga ggctattaaa gaagttttgg ttagcttgtt tgaaaatgat
    10501 gcgtcgtctg cacgtccgga aggctctatg cgagtttgtg aagtcttttg gtttacgcat
    10561 tcaagcaaac tggggaatgt ttcaagtgcg cgtgtctttg acttgttaga gtatcatcaa
    10621 tcaatagaag aaaaaagcac ttatgacgct tatcagattc atctaaatca agaaaaattg
    10681 gctaaatatg aagcgaaagg gttaacggtt gaaatcctag aaggactcta gtatggtcta
    10741 tgccgaagat gattatttaa tgctatcagg tattcagcat ttccaatttt gtaaacgtca
    10801 atgggcgttg atccatattg agcaacaatg gcttgataat gaagcgacag cgcatggaca
    10861 aattctacat actaaagcag ataatcctta cattaaagaa aaacgaaaag atcttttggt
    10921 ctcacgtgct atgcccattt cttctgcaga acttggactt tcaggaatta tggatgttgt
    10981 ggaattttat aaagatgatc aaggtgtgtc tttgagggga aaacgtggga aatggttacc
    11041 aaaagttgtg gaatacaagc gcggaaaacc taaaaaagat accagagata ttgtccagtt
    11101 ggtggctcag accatgtgtt tagaagaaac gctagactgc gacattaacg aaggttgtct
    11161 ttattaccat agtgtcaatc aaagagtgat tgttcctatg acatcagctt tgcgtcaaga
    11221 agtgaaggaa ttagccgcag agatgcatga ggtttatcag agtcaaatgc tacctaaagc
    11281 agcttatttt aaaaactgtc agctttgttc tttagtcgat atttgtaagc ccaggttgag
    11341 taaaaaaaca aggagtgtgt cgcgttacat caatgaggct atgaccagtg aggagatgga
    11401 cctatgaaga agttgctaaa taccttgtat ttgacgcaag aagattttta tgtcactaaa
    11461 gagggcgata acattgttat caagcaagaa ggtaaggttc tcaaacggtt tccgtttcgg
    11521 attattgacg gtattgtctg tttttcttat ttgggtgtgt cgcctgcttt ggtgaagtta
    11581 tgtacggaga atcagattaa tttatcgttt catacaccac aagggcgttt ttgtggtcgc
    11641 tatattggtt caaccaatgg gaatgtgttg ttgcgtagag aacattatcg tttatctgat
    11701 cgtgaggaat ctttggaata cgcaaagcgg tttattttgg ctaaaatttc caattcaagg
    11761 aaatacttgc tacgctttaa acgagatcat cgtcaacaga ttgataccaa gctttttgag
    11821 gctgttaatg acgaattgat atgggcttta gagatggttc aggcagcaga taataaagac
    11881 tctttaagag ggattgaagg ccaagctgct aatcagtatt ttcgcatatt taatgacctg
    11941 gtgttgacgg acaaaaaaac gttttacttc caaggtcgga gtaaacgacc acccttagat
    12001 tgtgttaatg ccctcttgtc ttttggttac agtttactga cctttgaatg tcaatctgcc
    12061 ttggaagctg tcggattaga cagttacgtt ggtttctttc acacggatcg tcctgggcgt
    12121 gctagtttag cgcttgattt agttgaagag ttccgctcat atattgtaga tcgttttgtc
    12181 ttttcattaa ttaataaagg acaacttcag aaaaaacact ttgaggttaa agaaaatggt
    12241 agtattttaa tgacggaaaa tggcagagct atttttattg atttgtggca gaagcgtaag
    12301 catactgagg tagaacatcc ttttacaaaa gagaaagtaa aacttatgtt attaccctat
    12361 gtacaagcgc agcttttagc taaggccata cgaggagatt tagaaagcta tccacctttt
    12421 atggtttagg agatgttata tgatggtttt agtcacttat gatgtaaata cggaaacacc
    12481 tgctggtaga aaaagattgc gtcatgttgc caaactctgt gtgaactatg gacaacgtgt
    12541 tcaaaattct gtttttgaat gttctgtgac acccgcagaa tttgtggata taaagcaccg
    12601 cttaacacaa atcattgatg agaaaactga tagtattcgc ttttatttat tggggaaaaa
    12661 ttggcagagg cgtgtggaaa cacttggtcg ctcagacagc tatgacccag ataaaggtgt
    12721 cttattattg taaaaatctc ttgtgcgaag ctagctttca cagaaacacc ttgcttgctc
    12781 gcgcaaaaat aacttaaaaa agaagcgaaa tggagataaa aaggcttaaa acacctctat
    12841 gtgtgccatc cgtttctcta taaactgtgt catttggcgc agtctcaccc ttcatgggtg
    12901 agtggattga aataagctaa acctatccta aaaatcaaag gcacagggtc tcacccttca
    12961 tgggtgagtg gattgaaata atgactatta tggggatatc tgccacgata tagtctcacc
    13021 cttcatgggt gagtggattg aaataccaat aattctaaag tcgctatctc tatctacggt
    13081 ctcacccttc atgggtgagt ggattgaaat aaaaacaaat cgttatcaat ttgcgtcttt
    13141 ccagcgtctc acccttcatg ggtgagtgga ttgaaatgat tcccccacga agcaaacaag
    13201 aattcacagt cgtctcaccc ttcatgggtg agtggattga aatacgtaaa atattaatca
    13261 gtgttgattt accagtaacc gtctcacttt tcatgggtga gtggattgaa ataaatttgg
    13321 cgaggagagc gtgaggcgaa tctagtgtct cacccttcat gggtgagtgg attgaaatat
    13381 tagcactggt tatattaagt gccgaggaaa aagtctcacc cttcatgggt gagtggattg
    13441 aaagatgaac ccgacctctc ttttaatgag gtcgggtttt tacatgcctt tagtcaacca
    13501 tttatctgat agcgtatcaa attcaagttt ttgcatgttc tagtctatgg tttgcgccac
    13561 tagctttcgg taaactaaga gtaacttaag agaggagtta tttatgacac tatcacttgt
    13621 attaatggta tcggtttttg gggcaggctt gttatcattt ttctctccgt gtatctttcc
    13681 ggtccttcct gtctatttag gcattttact ggatgcagat gattcaaaga cgattactat
    13741 atttggtaaa aaactctatt ggtatggcat tgtcaaaacc ttagccttta tttttggtct
    13801 atctactatt tttgtgattt taggttacgg ggctggtttt ttaggaaata tcttttatgc
    13861 ggtatggttt cggtacttac tgggagcctt ggtcattatc ttggggattc atcagatggg
    13921 cctcattacc attaagagct tacaatttca aaaatcactg actttccata ataacaagaa
    13981 tcgcaacggt ttgttcaatg catttatcct tggtttaacc tttagctttg gttggacgcc
    14041 ttgtgtggga cctgttttga gttctgttct agctttggtg gcttcagggg gaaatggtgc
    14101 ctggcaaggt ggcgttttga tgattattta tactcttgga ttgggcattc ctttcctgct
    14161 tatctctttt gcgtcaggca ttgttttgaa acagtttaac aagctcaaac cccacatact
    14221 tttactgaaa aaagtaggag gcgttctgat tatagtcatg ggaatcttgc ttatgacagg
    14281 aaccttaaat aacttagcac aactttttgg ataaaggaga aacacaatga aaaaaggact
    14341 attagtaaca actggtttgg cttgtctcgg gctactaact gcttgctcaa cccaagacaa
    14401 tatggctaaa aaggaaataa ctcaggacaa gatgagcatg gcagctaaaa agaaagataa
    14461 gatgtcaaca tcaaaggaca agtccatgat ggcagataaa tcatctgata agaaaatgac
    14521 caatgatggt cctatggcgc ctgatttcga actcaaaggc atcgatggta aaacctatcg
    14581 cttatcagaa ttcaaaggta aaaaagttta tttgaaattt tgggcctctt ggtgttcaat
    14641 ctgtttatca accttggcag ataccgaaga cctggctaaa atgtcagata aagactatgt
    14701 ggtcttaacc gtcgtctcgc caggtcatca aggagaaaaa tcagaagcag actttaaaaa
    14761 atggttccaa ggaacagact ataaggactt accagtcttg ttagatccgg atggcaagct
    14821 attggaagct tacggcgtca gatcttaccc aacagaagtt ttcattggaa gtgatggtgt
    14881 tcttgctaaa aaacacattg gttatgccaa aaaatcagac atcaaaaaga cccttaaagg
    14941 tatacattag gaaggaccag ctgataggca ttagaaaaaa ggagttgagt ttatggaaca
    15001 caaatggaaa ttatggctat ttatcgttgg agtggtcttt ttaataggcg gagcttctgt
    15061 tctcattcag aaaggtcaag ccaaggcttt tagtcaaggc aagcctcaac taccagctaa
    15121 aaaagtcaaa tccactaaag tagatcccaa tgcccctgtt gatcaaaaag tgatttacct
    15181 ggctggaggc tgtttctggg gcgttgaaga atatttttca caagtggatg gcgttctaga
    15241 tgcggtgtca ggctatgcca atggtagagg cgataccacc aactaccagt taattcatca
    15301 gactggtcat gcagaaaccg ttgaagtgac ctatgatgcg aaccgtatta gcttgaaaga
    15361 attattactg cattttttcc gaattattga cccaaccagt cttaataagc aaggaaatga
    15421 tcgcggtagt caataccgaa ctgggattta ctatactgac aaagcagatt tagctattat
    15481 tgatgaggtc ttcaaagaaa aagccaagga ttacaagaaa aaaatagtgg ttgaaaaagc
    15541 accattgaaa cactttatta aggcagaaga ctaccaccaa gattacctca agaaaaatcc
    15601 aaatggctac tgccatattg acatcaacca agcgacttat cctgtgattg atgaaagcaa
    15661 gtacccaaaa ccaagtgcag ctgagatcaa agcaaaactg tctgcagacg agtaccgagt
    15721 gacgcaaaaa aatgaaactg aaaaagcttt ttctaatcgc tactgggatt cttttgatgc
    15781 aggaatttac gtagacgttg tgacaggaga accacttttc tcttcaaaag acaaatttga
    15841 atcaggttgt ggttggccaa gttttagccg ccccatcagc ccagatgtta ttcgttacaa
    15901 ggaagataag agtttcaata tgacgagaac agaggtgcgc agccgttcag gggattctca
    15961 tctggggcat gtctttactg acggacctaa agaccaaggc ggccttcgtt actgcatcaa
    16021 tagcttgtct attaccttta ttccaaaagc tgatatggaa gctaagggct atggttattt
    16081 attatcatct gtagaatagg gttaggccta aaaatctgat ataatagaag aaagactagt
    16141 atgagagggc catgcaaggc cctttataaa gataaggaga caccgtgtgt actcattgtt
    16201 aattgtagaa gacgaatacc ttgtgcgcca gggtattcgt tctttggttg attttagcca
    16261 gttcaagatt gatcgggtca acgaagcaga aaatggccag ttggcttggg acttgtttca
    16321 gaaagagcct tatgatattg ttttgacgga tatcaatatg cccaaattaa atgggattca
    16381 actagcagaa ctcattaaac aggaatcccc ccaaactcat ctggtatttt tgacgggcta
    16441 cgatgatttt aactatgcct tatctgcttt gaaattaggg gcagatgatt acttgctcaa
    16501 acccttttcc aaggcagatg tagaagacat gttaggaaag ctccggaaaa aattagaact
    16561 ttccaagaaa acagaaacca ttcaggaatt ggttgagcag cctcaaaaag aagtatcagc
    16621 aatagcaatg gctattcatg agcggttggc agattctgat ttgaccctaa aaagcttggc
    16681 tcagcagctt ggttttagcc ctaattacct tagcgtcttg attaaaaaag agttagggat
    16741 gccctttcaa gattatctga tacaagaacg gttgaaaaaa gccaagcttc tcttgttaac
    16801 cagtaatctt aaaatctatg aaattgcaga gcaggtcggt tttgaggata tgaattattt
    16861 ttctcagcgc tttaagcaac tgataggcgt taccccaagt cagtataaaa aaggagacca
    16921 ggcatgaagc gttatcccct actggtccag ttgatttctt atatctttgt gatcgtaatt
    16981 gccctcatta ccacgcttgg tttgctttat taccagacga gttctcgtaa tatcaggcaa
    17041 ttaattgaac gtgatactcg gcagagcatt cggcaaagtt cccaatttat tgacgcttac
    17101 atcaagcctc ttaaagaaac aacttcggtg ctggcgaaaa atacggaaat tcaagccttt
    17161 gctagtcaaa ttcatcaaga aaatgacaag caggtccttc agctcatgaa gatggttctt
    17221 gcgaccaatt ccgatttaca agcagctgtt ctggtgacta aggatggtcg aacggtgtct
    17281 accaattctc agttgattat gaaaacctcc agtgatatga tggcagaacc ctggtataaa
    17341 gcagccatag accgtcaagc catgccaatc ttaaccccag ctcggcaatt atccctttct
    17401 tctaaaaaag aatgggtagt ttctgtgacc caagaggtag tggatgctgc tggtcataat
    17461 ttaggcgtgc taagacttga tattgcatac ccgaccatta aagcgtcttt agatcagctt
    17521 cagctaggcc gccaaggctt tgcatttatt gtgaatgata agcatgaatt tgtttaccat
    17581 cccaaaaaga gtgtttacag ttcttctaag gagatggctg cgatgaagcc ttatttggct
    17641 attaaaaatg gctacgccaa ggataaatca tcctttgttt atcaaagggt cattcccaac
    17701 agtcaatgga ctttgattgg tgttgcttcc ttggaccaat tgcaccgagt acaacgtcag
    17761 attttttggt ccttccttgg gatgggactt ctagcctgtc ttatttgtgg ccttacgact
    17821 attttagtct tacgtaaatg gattcgtccc attcaacaat tgcagcaagt tattcttgct
    17881 attcaaaaag gagatcgtca gttacgtgcc caagaaacgg gttctccaga attgacagac
    17941 cttgcccaac agtttaatgc gctcttagat caaattgata gcttgatggt tgccgttgcg
    18001 gataaggaaa aggcgattgg gcagtatagg ttacaagcct tggctagtca gattaacccg
    18061 cattttctct ataacacctt ggacactatt atttggatgg cagaatttaa tgacagcaag
    18121 cgcgtggtag aagtgaccaa gtctctagct aagtattttc gtttggccct taatcagggg
    18181 aacgaataca ttcgtttggc agatgaactg gatcacgtta gccaatacct ctttattcaa
    18241 aaacagcgct atggagacaa gctaagttat gacgttcaag gcttagatgt ctacgcagac
    18301 tttgttattc ctaagcttat cttacagccc ttagtagaaa atgctatcta ccatggcatc
    18361 aaagaagtcg atcgcaaagg catgatcaag gttactgtat ctgatacagc tcagcatctg
    18421 atgttgactg tttgggataa tggtaaaggc attgaagact cttcactgac caatagtcag
    18481 agcttgttgg ctaggggagg tgtgggcctt aaaaatgttg accagcggtt aaaacttcac
    18541 tatggtgaag gctaccacat gaccattcat agccagtcag accacttcac tgaaatacaa
    18601 ttaacccttc ctaaaatgca tgagttaatg gcagacgaca cacaggaaaa tgagtaaggt
    18661 tacaggaagt gcatgtcagc catttcccta agttagggta ttggagataa gaaccttaga
    18721 aaggatatca tgagtttagc atctgactat tttagccgac agacccccat agttgaaaag
    18781 ctagtggctt acggttttga gaagcgagac aacggttatt tctataatga acggtttatg
    18841 gaaggagaat ttgaggcgca gcttaggatt gatgaggctg gcaatatctg ggatcgggtt
    18901 attgattgtg atttggagga ggattatttg cctttacaac aagcagcctg gcaaggaacc
    18961 tatactgggc aggttagagc agcctatttg gaactcttgg ggcgcttatc agtagcctgt
    19021 tttgaagtca ctccttttca gtcaatgcaa gctaatagac ttgctaagca tatcaccaaa
    19081 gaatggtctg acccaatgga ctatcctttt gaaaagcacc cagaccttgc aacctatcgg
    19141 gtagggggca aatggtatgc tatgattttt tctctcttgg ctgataaatt agaccagata
    19201 ccagagagat tagtaggaca gacatgtgaa gtgatgactg tcaaagttaa tcccaaggct
    19261 ttccctcagt tactgcagca agaaggcatt tacccagcgt atcacatgtc aaagaaaaac
    19321 tggattagta ttattttgga tgataaggta acagatgata agctttggac gttagtgact
    19381 caaagtcgtc aactagtcaa cccaaacggt ctttctaatc ctaatggtcc tgattattgg
    19441 gttattccag ctaatttgaa atactatgat attgatgcag aatttgcagc caatgatgtg
    19501 attttatgga cccaaaaagc tggtattgcc gtgggagact atgtgctgat ttatataaca
    19561 gccccagtca aatccattcg ctatgtctgc caagttctag aaaccaatat acctaacgaa
    19621 ggctatcgta aaaatcctaa cattgacaaa ctcatgcgtc tccgaaaacg ccaacagtac
    19681 aaggatggtc tcttgtcttt tgaccttatg aaagaacatg gtgtagctgc agtgagaggg
    19741 ccaagacgat taagccctca attaattgca tttttaaagg aaaaagagta ttttaaagag
    19801 aacaactaaa ggagaataag agatggttga atttgtagca gatcaaagtt tttgggaact
    19861 ttttccagat gccaaactag gtgtcgtctt attaaaggat gttcagaatc aagcagagtc
    19921 accagaagat gtcaaacagc tcttagcaga tagccatgag ttagcaaaag cttatttaac
    19981 agcagacaat tttagtgata atcaggtggt tcaagtctac cgcaaggcct atcaacactt
    20041 taaaaccaaa aaaggagctc gctctagcat tgaagccctt ctcaagcggg tgtcaaatgg
    20101 tcaaagcatt ccgtctatta acccactagt ggatatttat aatgcggcta gtctacgttt
    20161 tggtttacct gttggagccg aagatagtga cagttttatc ggagatttgc gtctgacgat
    20221 aacagatggt ggtgacgact tttacttgat tggggatgct gacaataacc caaccttgcc
    20281 aaatgaactg tgttataaag atgatattgg agcggtttgt cgttgcctta actggcgaga
    20341 tggcgaacgc accatggtga cagaacacac caaaaatgca tttctgatta tcgaagctct
    20401 tgatcaagaa ggtcaaaata gattgcaaga agctcttgac ttcattgaag gcagcgcaaa
    20461 aatgtacctt catgcaatca cttctgtgca tgtgttggat aaagacaatc cacacgtgct
    20521 actttttaca gaaaactaga ttagcaggat gagctgtgta aaatagaagg ttaacaaaaa
    20581 agcgagtaaa cgaatgtcct gttctttagg ttttcgttta ctcgcttttt gatgagatct
    20641 tactgctgcc ctagcaaaag gtgaggatga ggtcgtatat cacatagagg ttttctgtta
    20701 agccttattt gttactaaag aagaatggtg gtgtgtaatg actaagggtc tcgtaacaag
    20761 ccttggcttg ctcattgtct tcacagacaa tcaagcaagt atcatcacca cagatcgttg
    20821 ctacaatttc tggaatttgc atggcatcta aaatggagcc aaaggattgg gctagtccag
    20881 gtaacgtttt taaaataatt tgatgttgaa ctatctttaa catgaccaaa gcgtcttcca
    20941 tgtaaaaacg aagacgatgt tcccagcggg tttgtgaaat ggctaacgct tcatagtgtg
    21001 tgtcgtttcc agaagttact ttgaccaggt tcaattcctt catgtcacgt gatagggtag
    21061 cctgagtgat cgtgatgcca tttttttgca accgttcttg taattcttgc tgtgtatgta
    21121 tagttgtctc agagatgagg gaacgtatga gttggtgacg cgtctctttc ttattcattg
    21181 ttataatctt cctttaacgt aattgataac aatccgagta tagcaaaaag aggagaaggt
    21241 gtaaagtttt tgatgaaccg cttacaaaat ggctttttta tggtaaaata aagatgatgt
    21301 ttagaaaaag gagatttgtg tgattcgaag agaagattat caatacctta gaaaactaaa
    21361 cgattttcgc tacttttcta ttgaacaatt tgacaaaatt gtagggcaga tggaattccg
    21421 aaaagctaaa aaggaccata tccttttttt tgagggagat aaaagagata agctttttct
    21481 agtgacctct ggttatttca aggtggagca gtccgatcaa tcaggaacct tcatgtatac
    21541 ggatttcatc agacacggca ccatttttcc ttacggtggc ctctttacag atgattatta
    21601 ccatttttca gtggtagcca tgacagacgt gacttatttt tactttccag tcgatttatt
    21661 tgaagattat tcgcttgaaa atcgtctaca aatgaaacac ttatatagta agatgtctaa
    21721 gctgctcgag ttacatgagt tgagggtccg aaacttgatt acatcaagtg ctagttctcg
    21781 tgtgattcaa tctctggcga ttttgctggt tgaaatgggt aaagacagtg ataccttacc
    21841 ttttcaatta acaacaacag atattgctca aataagtggg acgacaagag aaacagttag
    21901 ccatgtgctt agagatttga agaagcaaga attgattacc ataaaaggta agtatttgac
    21961 ttatctagat aaagactatt ttttacaata caca
//
LOCUS       DAUGFP010000025        21691 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.025, whole genome shotgun sequence.
ACCESSION   DAUGFP010000025 DAUGFP010000000
VERSION     DAUGFP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21691
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.025"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            169..2547
                     /locus_tag="VH663_001007"
     CDS             169..2547
                     /locus_tag="VH663_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEQ8174217.1"
                     /translation="MKTKKVIILVGLLLSSQLTLIACQSRGNGTYPIKTKQSRKGMMS
                     NKIKPIKKSKKTNKTHKGVAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQQAASQGTSKVADSHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSPVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAHVADQYDLAKKAEKPAETHHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVEIQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPDPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTPFPSNEKDPNKM
                     KEWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGIGFTPIKDISPVLQFKKLKRLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLRQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPKLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHAYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            3083..4279
                     /locus_tag="VH663_001008"
     CDS             3083..4279
                     /locus_tag="VH663_001008"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ8174218.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQICQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKIDALVE
                     AVKTVL"
     gene            4453..5712
                     /locus_tag="VH663_001009"
     CDS             4453..5712
                     /locus_tag="VH663_001009"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ8174219.1"
                     /translation="MRKIIINGGKVLSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
     gene            6070..6807
                     /locus_tag="VH663_001010"
     CDS             6070..6807
                     /locus_tag="VH663_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ8174220.1"
                     /translation="MGKEIKVKYFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERAIDELKKQAIEDKEATTAIEAASSDALEALADQA
                     DALQSEEAAVVQSDTAASDALEALADQTDALQSEEAAVVKADTAASDALEALADQTDA
                     VQSEEASVVKADTAASDAWEKAATPTALDVKKTKDTTPVVKKEERQNVNTLPTTGEES
                     NPFFTAAALAIMVSTGALVVSSKCKEN"
     gene            7045..7587
                     /locus_tag="VH663_001011"
     CDS             7045..7587
                     /locus_tag="VH663_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8174221.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            7596..8879
                     /locus_tag="VH663_001012"
     CDS             7596..8879
                     /locus_tag="VH663_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ8174222.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCENSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            8895..9755
                     /locus_tag="VH663_001013"
     CDS             8895..9755
                     /locus_tag="VH663_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEQ8174223.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTGSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            9757..10722
                     /locus_tag="VH663_001014"
     CDS             9757..10722
                     /locus_tag="VH663_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEQ8174224.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            10833..12116
                     /gene="aroA"
                     /locus_tag="VH663_001015"
     CDS             10833..12116
                     /gene="aroA"
                     /locus_tag="VH663_001015"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEQ8174225.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSHLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            12109..12600
                     /locus_tag="VH663_001016"
     CDS             12109..12600
                     /locus_tag="VH663_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEQ8174226.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFANDNSVIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            12808..14259
                     /locus_tag="VH663_001017"
     CDS             12808..14259
                     /locus_tag="VH663_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ8174227.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDTPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            14308..15699
                     /gene="rlmD"
                     /locus_tag="VH663_001018"
     CDS             14308..15699
                     /gene="rlmD"
                     /locus_tag="VH663_001018"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ8174228.1"
                     /translation="MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVD
                     NALPEEVIDMRVLKVNKNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQ
                     LTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
                     SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVHRGHYSGE
                     MMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTI
                     TDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFA
                     KTVKAVYGVEVIEAAVRDAQHNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVD
                     PPRKGLTESFIQASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQT
                     HHVECVSLLEKRS"
     gene            15851..16632
                     /locus_tag="VH663_001019"
                     /pseudo
     CDS             15851..16632
                     /locus_tag="VH663_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            16717..17118
                     /locus_tag="VH663_001020"
     CDS             16717..17118
                     /locus_tag="VH663_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEQ8174229.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(17223..18707)
                     /locus_tag="VH663_001021"
     CDS             complement(17223..18707)
                     /locus_tag="VH663_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEQ8174230.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLEKNGIASYLHYPIKGYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIKASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            19081..20301
                     /locus_tag="VH663_001022"
     CDS             19081..20301
                     /locus_tag="VH663_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8174231.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLGL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGVAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     SIGQLKAERYQQLR"
     gene            complement(20421..20786)
                     /locus_tag="VH663_001023"
     CDS             complement(20421..20786)
                     /locus_tag="VH663_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEQ8174232.1"
                     /translation="MTIYSHTYETRPNHSAKAMSSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            20901..21632
                     /locus_tag="VH663_001024"
     CDS             20901..21632
                     /locus_tag="VH663_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ8174233.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKTVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVVGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKGVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
BASE COUNT         6814 a         3803 c         4590 g         6484 t
ORIGIN      
        1 ggaaaagaaa agttagtttt tttgtgtaat gctactaaca acagaaacta ttgtttgttg
       61 aagcttcaga gctccaactc gtcaacaact aatagttatc ctaaaaagag cttgaggaaa
      121 aacaagtgat gtgatatact taaccagtta aataaaaagg agggttgaat gaaaacgaaa
      181 aaagttatta ttttagttgg tctattgtta tcatctcagt tgactttgat agcttgtcaa
      241 tcacgaggta atggtacata tcccattaaa acgaaacaat cacgtaaggg aatgatgtca
      301 aacaaaatta aaccgattaa aaaaagcaaa aagacaaaca agactcacaa aggtgtggcg
      361 ggtgtcgatt ttcctacaga tgatgggttt attttaacca aagactcaaa aatcttatca
      421 aaaacagatc agggaatcgt tgttgaccat gatggtcatt cgcattttat tttttatgcc
      481 gatttaaagg gaagtccatt tgaatacctt attccaaaag gagcaagttt agctaagcca
      541 gctgttgctc agcaagcagc tagtcaaggg acttctaaag tagcagattc tcatcaccat
      601 tatgaattta acccagcgga tattgtggct gaagatgctt taggctacac ggttcgccac
      661 gatgatcact tccattatat tttgaagtca agcttatcag gtcagacaca ggcacaagct
      721 aaacaggttg ctactcgctt gccacaaacc agtagccctg tttcaacagc tacagctaat
      781 ggtattccag gcttgcattt cccaacctca gatggttttc aatttaacgg tcaaggtatt
      841 gttggggtaa caaaagacag tattttagtg gaccacgatg gtcacttaca tcctatttct
      901 tttgcggacc ttcgtcaggg tggctgggca catgtggcag atcaatacga tctcgctaaa
      961 aaagcagaaa agccagcaga aacccatcac acaccagagc tatctgaacg tgaaaaggaa
     1021 taccaagaaa aattagctta tttggcagaa aaattgggga ttgatccatc aactattaaa
     1081 cgtgtggaaa tacaagacgg taaacttggt ttggaatacc ctcaccatga ccacgcacac
     1141 gtattgatgt tatctgatat tgaaatcgga aaagacattc cagatccaca tgctattgag
     1201 catgcccgtg aattggaaaa acataaggtt ggaatggata ccttgcgtgc cttagggttt
     1261 gatgaagaag tgattttgga tatcgttcgc actcacgatg ctccaacccc attcccatca
     1321 aatgaaaaag atccgaataa gatgaaagaa tggttagcaa cggttattaa acttgacttg
     1381 ggcagccgta aagatccttt gcaacgtaaa ggactttcac tgttacccaa cttagaaact
     1441 ttaggaattg gctttacacc aatcaaagat atctcacctg ttttgcaatt taaaaaattg
     1501 aaacggttac taatgacaaa aacaggggtg actgattata gatttttgga taatatgcca
     1561 cagttagaag gcattgatat ttcacaaaac aatctcaaag atattagttt cttgagcaaa
     1621 tataaaaact taactctagt agcggctgct gataatggta ttgaagatat taggccgctt
     1681 ggtcaattac caaatctcaa attcctcgta ttgagtaata ataagatttc tgatttaagc
     1741 ccactggcat cgttacgtca attgcaagaa ttgcacattg ataataatca gattacagat
     1801 ttaagccctg tttctcataa agaatcattg acggttgttg atttatcaag aaatgctgat
     1861 gttgacttag caacacttca agcccccaaa ttagaaacgt taatggtcaa tgataccaag
     1921 gtttctcatt tggatttctt gaaaaataat cctaatctat ctagcctatc tattaaccgt
     1981 gcgcaattgc aatctcttga gggtattgaa gcaagtagcg tcattgtcag agtagaagca
     2041 gaaggtaacc aaattaaatc gcttgtgctt aaagacaagc aagggtcact tactttcttg
     2101 gatgtgacag gcaaccagtt gacttctcta gaaggtgtta ataattttac agcacttgac
     2161 attttaagcg tgtctaaaaa ccaattaaca aatgtcaacc tatctaaacc caataagaca
     2221 gttactaaca ttgatattag tcataacaat atcccattgg cagaccttaa attgaacgag
     2281 caacatattc cagaagccat tgcgaaaaac ttcccagcgg tttacgaagg ttctatggta
     2341 ggtaatggaa cagctgaaga aaaagcagct atggctacta aggcgaaaga aagtgctcaa
     2401 gaagcatcgg aatcacatga ctacaaccat aatcatgcct atgaagatga agaaggtcat
     2461 gctcacgagc acagagacaa agatgatcac gaccatgaac atgaggatga aaatgaagct
     2521 aaagatgagc aaaaccatgc tgactaaact aatctaagaa taacccgatc caactgaatc
     2581 gtcttaatga caccattgtt gactatcaag aaaacctcaa gaataacctt gaggtttttt
     2641 tgaaataaat aggataatga gtgaaaagaa tggcacctaa atgactaaaa catgttataa
     2701 tagattgtta ttgagtcccg ataagacggt ctgttgaacg aaatgttttg agacgttgtt
     2761 gacttcattt ggggaacagt cgttctctct ttagctaaat tacttcgtct aaggcttatt
     2821 ttgtggtgac atcctctcaa caaggagacc ggattctttg taactcaacg ctattgtcag
     2881 acattctttg tctggttaat ggacgctagc cactaggtgg actagctagt tgccttacta
     2941 gtaaaaccgt aactgattag ctaagttggt tttggccact tgacttgctc aatcagctag
     3001 gaagaaaggg gtaggcaact tcttgctttg tggtcaatgt gactgaaaaa ggaagaatca
     3061 acatattagg ggggacattt ttatgtcaga acgtaaactt ttcacgtctg agtccgtatc
     3121 tgaagggcat ccagataaga ttgcagacca aatttcagat gctattttag atgccatttt
     3181 agcagaagat ccagaagccc atgtggctgc agaaacttgt gtttacacag gttctgttca
     3241 tgtttttgga gaaatctcaa caacagcata cattgatatt aaccgtgtgg tccgcgacac
     3301 cattgcagaa attggttata cagaagcaga atatggcttt tcagcagaat cagtgggagt
     3361 acatccatca cttgtagaac aatctggaga tatcgctcag ggggttaatg aagctctcga
     3421 aagcagagaa ggtgatactg atgaccttag ccatattgga gcgggtgacc aagggctcat
     3481 gtttggtttt gccatcaatg aaacaccaga attgatgcct ttaccaattt ccttatctca
     3541 ccaattagtg cgtcgcttgg cggaattacg taagtcaggt gagattagtt atctacgtcc
     3601 agatgctaaa tctcaagtga cagttgagta cgatgaacac gataaacctg ttcgtgtgga
     3661 tacagtcgtt atttctactc agcatgatcc agaagctact aatgaccaaa tttgtcagga
     3721 tgtgattgaa aaagtgatca aggctgtaat tccagcagat tatttagatg atgacacaaa
     3781 gttcttcatt aatccaacag gacgttttgt tattggaggt ccgcaaggag actctggatt
     3841 gaccggacgt aagattattg tggataccta tggaggctat tcacgtcatg gtggcggtgc
     3901 tttttcagga aaagatgcga caaaagtgga ccgttcggct tcttacgctg ctcgttatat
     3961 tgctaaaaac cttgtggcag caggccttgc gacgaaagct gaagtccaac tagcttatgc
     4021 gattggagtt gctcagcctg tgtctgttcg cgtggataca tttggaactt caacggttcc
     4081 tgaagctgtt ttagaagctg ctgttcgtca agtctttgat ttacggccag caggcattat
     4141 tcaaatgctt gatttaaaac gcccgattta caaacaaaca gctgcctatg gtcacatggg
     4201 gagaacagat attgatttgc catgggagcg tttaaataag attgatgctt tagtagaagc
     4261 tgtaaaaaca gtattataaa cgacaacttt attaaaaaag tagagtgagt gaaaaaatct
     4321 tactctgctt ttttgttatt aaataagcct ttaaaaggga tgattaaaaa ttaagacgtc
     4381 aataattgtc aggcttcatg atttatgata aaatgaaaag gttgaattct agtataagaa
     4441 agttttgaat gtatgcgtaa aattattatt aatggcggaa aagtcttatc tggagaagtg
     4501 gcagtttcag gggcaaaaaa cagtgttgtt gcgcttattc cagcaatcat cttagccgat
     4561 gatattgtga ttcttgatgg tgtacctgct attagtgatg tagatagtct gattgaaatc
     4621 atggagctta tgggagcaac agtaaactat catggtgata ctttagaaat tgaccctaga
     4681 ggggtgcaag acatcccgat gccttacgga aagattaata gtcttcgagc ttcctattat
     4741 ttctacggta gtcttttagg ccgttttgga caagcagttg ttggcttacc tggtggttgc
     4801 gatttaggac caagaccaat tgacttgcat ttgaaggcct tcgaggctat gggagttgaa
     4861 gtgtcttatg agggtgaaaa tatgaattta tcgacaaatg gtcaaaaaat tcatggcgct
     4921 catatctata tggacaccgt tagtgtcggt gctacaatta atactatggt tgcggcaaca
     4981 aaagctcaag gtaaaacggt tattgaaaac gctgcacgtg agccagagat tattgatgtg
     5041 gctactcttt tgaataacat gggtgcgcat attcgtggag caggtacaga tattattacg
     5101 atacaaggtg ttcagaaatt gcatggtact agacaccaag ttattcctga tcgtattgaa
     5161 gcagggactt atatcgcttt ggcggcagct attggaaaag gtgttaagat cactaatgtt
     5221 ctttatgagc atttagagag tttcattgct aagttagaag aaatgggtgt ccgtatgacg
     5281 gtagaagagg atgctatctt tgttgaaaag caagaatccc tcaaggctat tacaattaaa
     5341 acctctcctt accctggttt tgccactgat ttgcaacagc cattaacgcc cttgttgtta
     5401 aaagcagatg gtcgtggaac gattattgac actatatatg aaaagcgcat taaccatgtg
     5461 ccagagttga tgcgcatggg agcagatatc tcagttattg gtggtcagat tgtttatcaa
     5521 gggccgtctc gcctgacagg agctcaggtc aaagccactg atttacgtgc cggagcggct
     5581 ctagtgactg ctggtctaat agctgaaggc aagacagaaa ttactaatat tgaatttatc
     5641 ttacgtggct atgctagtat cattgcaaaa ttaacagcac taggggcaga tattcaatta
     5701 atcgaagact aaagagaagc taacgcttct cttttttata ttacatgtgc ttaggctaat
     5761 aatacttagg tgcatacttt gttgcatttt atggcttttt aactgtcata aaaaatataa
     5821 aaatgatatc actaactaat attattaatt ttaatgctaa aataatgagt tgacggtagt
     5881 aaagtgataa ataaacactc cttaaaatct tattttatta atttttttaa aaaataatat
     5941 ataaattaga taataaactt tattttatgc aattaatctg tataataaaa gatggaatta
     6001 ttaattataa tttccaaatt atcggtaatt taatatgcta atgcatataa aaataaaaaa
     6061 ggagaaacaa tgggaaaaga aataaaagtg aaatactttt tgcgtagatc agcttttgga
     6121 ttagttgcgg tgtcagcatc agtattagtc ggttcaacag tatctgctgt tgactcacct
     6181 atcgaacagc ctcgaattat tccaaatggc ggaaccttaa ctaatcttct tggcaatgct
     6241 ccagaaaaac tggcattacg taatgaagaa agagccattg atgaattaaa aaaacaagct
     6301 attgaggata aagaagctac gacagctata gaagcagcaa gttcagatgc cttagaagca
     6361 ttagcggatc aagcagacgc tttacaatca gaagaagctg cagtagttca atcagatacc
     6421 gctgctagtg atgccttaga agcattggcg gatcaaacag acgctttaca atcagaagaa
     6481 gctgcggttg ttaaagcgga taccgctgct agtgacgcct tagaagcatt ggcggatcaa
     6541 acagacgctg tacaatcaga agaagcttca gttgttaaag cagataccgc tgctagtgac
     6601 gcctgggaaa aagcagcaac tccaaccgct ttagatgtta agaaaactaa agatacaaca
     6661 cctgtagtta aaaaagaaga aagacaaaac gttaataccc ttcctacaac tggtgaagag
     6721 tctaacccat tctttacagc tgctgcgctt gcaataatgg taagtacagg tgcgttagtt
     6781 gtaagttcaa agtgcaaaga aaattagttg ctatttgttc tagcaaatga aacaagagaa
     6841 tcgaaagatt ctctttttct gatatttaaa aatgctacag tttataatac cttgaaagac
     6901 taaatgttct caaactcatt agaacaagct ttaataaaac taacccagtt gtatcaaagg
     6961 atagataaag actaagactg ttatagaata tttgcaaaag atttgttata atggacccaa
     7021 tacaggtatt gtgaaaggta taatatggat atttggacaa agcttgcagt gtttgctttt
     7081 tttgagactc cgaaagtaat attacggcct tttcgctatg aagatcattg ggatttttac
     7141 agcatggtta acgacactaa aaacctttat tatgtttttc cagaacaaaa aactaaggca
     7201 gcaagtgact atcttttagt acatagtttt ataaagtttc ctttaggtca gtgggcaata
     7261 gaagataaag caacccacca agtaataggt tctattagaa ttgagcatta tgatgctaaa
     7321 acgcgttgtg ctgatattgg ctatttttta aactatgcct tttggggaca aggaattatg
     7381 acagaagtcg taataaaact tgtttattta tcttttcacg aatttggtct caaaacgttg
     7441 cgtattataa ctcatttaga aaataaggcc agccagaaag tagctaaaaa agcaggtttt
     7501 caactaaaaa cctgttttaa aggaagtgat cgtaatactc ataaaatctg catttataaa
     7561 atgtaccaac taactaatga taggtgataa tcattatgag caaacatcaa gatattttag
     7621 attatttaga aaaacttgct attggtaaaa aagtgagtgt tagaagcatt tcaaaccact
     7681 taaaagtaag cgatggaaca gcctaccgtg ctattaaaga ggcagaaaat cgaggtattg
     7741 tcgaaaccaa accaagaagt ggaactgttc gtattgaaaa aaaaggacgg gttcgtattg
     7801 accgtctgac ctattccgaa attgctcgga tcagtgattc ggaagtgcta gcgggacatg
     7861 ctggtttagg acatgaattc agtaggtttt caattggtgc tatgactcaa caaaatattc
     7921 gtcgttactt ggtcaaagga gggttattaa tagttgggga tcgtgaaacg attcaactct
     7981 tagctcttga aaaccataat gccattttgg tgacgggcgg atttccagta tccaaacgtg
     8041 ttattgaaat ggcaaataac caacgaatcc cagttatggt aactcattat gataccttta
     8101 ctgtggcaac aatgattaat cacgcgctat ctaatattcg tattaaaaca gatctcaaaa
     8161 cagttgaaca ggtaatgata cctattacag attatggtta cctttgcgaa aacagctctg
     8221 ttgaagaatt caatacatta ataaaaaaaa cacgacaagt caggtttcct gtattagatt
     8281 ataaaagaaa agtgattggt gtagttagta tgcgtgatgt ggtggatcag ttgccaacaa
     8341 ctaagcttac caaggtcatg tctaaaaatc caataacggc tagaccaaat actagtcttg
     8401 ctaatattag ccagaaaatg atctttgaag atttaaacat gttacctgtc actgacgaag
     8461 aaaacaattt acttggtatg attactcgta gacaagcaat ggaaaatttg ccaaatcacc
     8521 aacccaataa cccctatact tatagtgagc aaatactttc taaccttgaa gaaacggttg
     8581 attattacca agtagtagtt gagccaacaa tgattgatag tgctggtaac atgtcaaacg
     8641 gtgttatttc agagttctta aaggaaatta gtatcagagc cctcacgaaa aagcaccaaa
     8701 aaaatatcat tatcgaacaa atgatggtat actttttaca tgctattcaa attgaggatg
     8761 aattaaaaat atatcctaaa atcattactg aaaatagacg aagcagcacc attgatattg
     8821 aaatttttgt ggatgatcaa gtgattgcta aagctattat tacaacgaaa ataaattaga
     8881 ggagtaacaa aacaatgata acattaaaat cagcccgtga aatagaagcg atggatagag
     8941 caggagactt cttagcaggt atccatattg gcttaaggga tattattaag cctggtgtgg
     9001 atatgtggga agtggaagca tatgttcgtc gccgttgtaa agaagacaac gttcttcctt
     9061 tgcaaatagg ggttgatggt cacatgatgg actatcctta tgcgacctgt tgtggtctaa
     9121 atgacgaagt agcacatgcc tttccccgtc attacattct gaaagaaggg gatttgctta
     9181 aagttgacat ggtactcagt gagcctttgg ataaatcaat tgttgatgta gcagctttag
     9241 attttgataa tgtgcctgag atgaagaaat ggacaggatc ttatactggt ggtttagctg
     9301 actcttgttg ggcttatgct gttggaacac cgtctgacga aataaagcaa ttaatggatg
     9361 tcaccaaaga agccatgtac cgtggtattg aaaaagcagt aattggcaat cgtattggtg
     9421 atattggcgc agctgttcaa gaatacgctg aaagcttcgg ttatggtgtc gtgcgtgatc
     9481 ttgttggtca cggtgttggt cctacaatgc atgaagagcc aatggtacca aactatggaa
     9541 cagcaggtcg tggacttcgt ctaaaagaag gcatggtctt gactgttgag ccaatgatta
     9601 atacaggaac ttgggaaatt gataccgaca tcaagacagg ttgggctcat aaaaccctag
     9661 atggtggttt atcttgccaa tacgaacacc aatttgtgat tactaaagat ggtcccgtta
     9721 ttttaacaag tcaaggagaa gaaagaactt actaaaatgg cagaaaaaaa atggtttgat
     9781 aaggtactgt ccaagtggca gtatgaaccc attcaagtct ttatgaggca tttacaaagt
     9841 gcagaaatgg atttatctgc cattgcggtt gcgtattacc ttatcttaac ggcctttcca
     9901 ttaattgtta ttgcagccaa tatttttcct tacctcaata ttgatattgc tgatttgtta
     9961 cgtttaatga agcaaaattt gcctaaagat atttttagac cggcttctgc aattgtagaa
    10021 aatattttct ctaaaccgtc tggaagtgtt ttgggagtag cgactttgac aggattatgg
    10081 acaatgtctc gaagtttgac atcgctgcaa aaagctatta ataaagccta tggcgcttct
    10141 cagcatcgag atttctttat cggacacttg gttggtttgt tgaccagttt aattatttta
    10201 tttttattag cctttgcctt aatcttttca acattttcta aagctgctat tcaagtgctg
    10261 gataagcatt accatttgag tgataatatc acaacgattt tcttactcct cattcaacct
    10321 attacagttt tgattatttt tgttggcttg atgctactat attttttatt acccaatgtt
    10381 aagattaaaa aaattcgcta cattttacca ggaaccctat ttacatcttt tgtgatgaca
    10441 tttttgagta atctggttgg caattacgtt gtgtataatg ttgagcggat ggtagatatt
    10501 aaaatgtttg gttcagttat gatttttatc atcatgctat ggtttatttt cttagcaaga
    10561 atcctgattt taggagctat atttaatgcg acctatcaag aaatgtcctt ggggaaatta
    10621 gaaggtcgta gtggtgatat gatcgctatc cttaaaaaaa cacttgggaa tgattcagat
    10681 ctatctcctt cacagtctat cgaagatagt cacactgatt gatacaaaaa agataataag
    10741 tcgtggagtg agaaactatt ttcttgcttc attttttatg aggataaata gaaaatcatt
    10801 gatcttagca aatagaaagg cagataaaac ggatgaaatt gcgaacaaat gcaggaccat
    10861 tacagggaac gattcaagtt ccaggagaca agtccattag tcaccgggca gtcatattag
    10921 gagctgttgc taaaggggaa acacgagtaa aaggactctt aaaaggagaa gacgtgcttt
    10981 ccacgattca agcctttagg aatttggggg tccgtatcga agaaaaagat gaccagcttg
    11041 tgatagaagg tcaaggtttt caaggattga ccgctccttg tcagacactt aatatgggaa
    11101 attctggaac ctctatgcgc ctcattgctg gcttattggc aggtcaacct tttagtgtca
    11161 aaatgattgg cgatgaaagc ttatccaaac gtcctatgga tcggattgtt tatccgttaa
    11221 agcaaatggg ggttgagata tctggagaaa cagataggca atttcctcca ctacaattgc
    11281 aaggtaatcg taatttacaa ccaatcacat atactttgcc tatctcttct gctcaggtta
    11341 agtcagctat tttgttagca gctttgcaag caaaaggaac aacacaggtt gttgaaaaag
    11401 agattactcg aaaccataca gaagaaatga ttcaacaatt tggtggacga ttgatagttg
    11461 atggtaaacg cattactttg gtgggacccc agcagctcac tgctcaagag ataactgtcc
    11521 ccggagatat ttctagtgca gctttttggc ttgtcgccgg cttaattatt cctggttcgg
    11581 agctcctttt aaagaatgtt ggagtcaatc cgacacgaac agggattctt gaagtggttg
    11641 aaaaaatggg agcacagatt gtctatgaag atatgaataa gaaagagcaa gtaactagta
    11701 ttcgagtggt atattctcac ttgaaaggaa ctatcatatc aggaggtctt atccctcgat
    11761 tgattgatga acttcccatc attgcacttc ttgcaaccca agcacaagga acaacctgta
    11821 ttaaagatgc tcaagaatta cgagtcaaag aaacagatcg tattcaagta gtaactgata
    11881 ttcttaacag tatgggagct aatatcaaag cgacagcaga tggcatgatt atcaaaggcc
    11941 ctacggtact atatggggct aacacaagta cctatggaga tcatcgtatt ggtatgatga
    12001 cagcaattgc agccttgctt gttaaacaag gacaggtcca tttggataaa gaagaagcca
    12061 tcatgacgtc ttatccaacg tttttcaaag atttagaaag gctgtgtcat gactaaagtg
    12121 ttattgggat ttatgggagt ggggaaaaca acggtttcga aacacctaag tatgcactgt
    12181 aaagatatgg atgctattat tgaagctaaa atcggtatgt ctattgcagc tttttttgaa
    12241 cagcatggag aaatagcatt tcgaacaatt gaaagtcaag tgttaaaaga tctattgttt
    12301 gcaaatgata atagtgtcat cgtaacaggt ggcggtgtag tggttttaca agaaaatcga
    12361 caattactga gaaaaaatca ccagcataat attttattgg tagcttcttt tgaaactttg
    12421 taccagcgtt taaaacatga taaaaagtct caaagacctc tatttcttaa gtactctaag
    12481 gaagcatttt atgaatttta tcaacaacgt atggtatttt atgagggact ttcagattta
    12541 gtaatcagag ttgaccatcg tacgcctgaa gaagttgcca acatcattga gggttattag
    12601 taggtcattg tcgtaatctg atatttatgg ttttaaacag gtatctgtct aaagcacttc
    12661 ttatggcaag ttggctttat taaatattag gaaataatct tgattattct ataatgatta
    12721 aaattttgag aggacaatct ggctaatata ggttataatg gtagtattcg ttaaaagtgc
    12781 aattgtaata ctaagagatg ataagatatg acaaaatacc ccatgggtgg ccttagtcac
    12841 catgaagaat tacgttattt ttatctccta aggaatttga gttaccttag tgagaatgaa
    12901 aaaaaagagt ttgctttttt aaaatctaaa ttggaaattg gtcgagctta tgctccttct
    12961 aaacagcatt ataggaagtc aaaacggcaa gaaccttact ttgaagatga ctactataac
    13021 gactactcac caaatgattt actagaagat gatgacgtca atcatgactc ttcttttgtt
    13081 ccatatggtt taccgatata tcctaaagaa gacagatatt taaacaaaaa aacaaaactt
    13141 acagctcgta gacctataga tactccgcag cctattgatg aagacgatgc ttttttaaca
    13201 gagtctgttg ctcgttgtgc tcttcctaga tctcaaaaaa gaaagcataa gaaaaaagga
    13261 tgtatgaaat ggttttttaa cattcttggc ttattgctaa tgactgtgct tatggggtta
    13321 gggttgatgt ttgcaaaagg ggtttttgat atttctacta ataaagctaa ttacaaacca
    13381 gctgtttccc aagcttttga tggtcaagaa actcaagatg gtacgaacat tctggttttg
    13441 ggttctgacc aacgagttac tcaaggttct acagatgctc gcacagatac cattatggtt
    13501 gtcaatgtcg gcaaccatgc taaaaaaata aaaatggtct cttttatgcg tgacactttg
    13561 attaacattc caggatacag ttataatgat aattcgtatg atcttaaatt aaatagtgca
    13621 tttaatcttg gagagcaaga agatcaccac ggagcagagt acgttcgacg ggccttaaaa
    13681 cataattttg atatcgacat taagtattat gtgatggtag attttgaaac atttgcagaa
    13741 gctattgaca ccttgtttcc aaatggtgtt aagattgatg ccaaatttgc gacagtaggt
    13801 ggtgtagctg ttgactccgt agaagttcca gatgacctgc gaatgaaaaa tggggttgtt
    13861 cccaaccaaa ccatcgaagt tggagaacaa cgcatggatg gtagaacctt gctcaactat
    13921 gctcgtttcc gtaaagatga cgaaggagat ttcggacgaa ccgtccgtca gcaacaggtt
    13981 atgtcagctg ttatgtccca aatcaaagac ccaactaaac tctttacagg gtctgcggct
    14041 attggtaaaa tctatgcgct gacttcgacc aatgtttctt ttccatttgt tgttaaaaat
    14101 ggtgtttctg ttttaggaag tgggaaaaat ggagttgagc acgtgaccat cccagaaaac
    14161 ggggattggg ttgacgaata cgatatgtat ggaggacaag ccctttacat agattttgat
    14221 aagtatcaga aaacgttagc caaattaggt ctgcgctaag atatggtata atggagagta
    14281 actaaagttg ctcttctttt ttgagcaatg gttagcccta gaaaaggaaa aagaataaga
    14341 atgttgaaaa aaaatgatat tatccaagtt gctatttcag atttaagcca tgagggagca
    14401 ggtgtcgcta aacacgatgg ttttgttttc tttgttgata atgccttgcc agaagaagtg
    14461 attgacatgc gtgtcttaaa ggtcaataaa aacagcggtt ttggcaaagt agaagcctat
    14521 cattacctgt cgccagctag aaatgcagat gtcaatttaa cttatttgcg aacaggcatt
    14581 gcagaccttg gacatctaac ttatgaagac caactgactt tcaaaaagaa acaagttcag
    14641 gacagcctct ataaaattgc tggaatcagt gacgtgacag tcgaaagcac gattgggatg
    14701 acagaacctt tagcttaccg aaataaggct caagttcctg ttagacgtgt taatggtcag
    14761 ctagaaacag gctttttccg caagcattct catgacttga ttcctatttc ggattattat
    14821 attcaggaca aagagattga tcgtctcatt aattttaccc gtgatttatt acgccgcttt
    14881 gatattaaac cgtatgacga aacagaacaa acaggtctct tgagaaatat tgttgttcac
    14941 cgaggccatt actctggaga aatgatgctg gtcttagtga ctacacgccc aaaagttttc
    15001 cgtgttgacc aagtcattga aaaaatcgtt gaagcttttc cagcagtggt gtctattatt
    15061 caaaatatta atgataagaa caccaatgca atctttggta aagatttcaa aacgctttac
    15121 ggcaaggata ccattactga tagcatgctt ggaaacaact atgccatttc tgcccagtct
    15181 ttctaccaag tgaatacggt tatggcagaa aaactctatc aaacagctat agccttttca
    15241 gacctttcta aagatgacat tgtgattgac gcctactcag gtatcggaac cattggccta
    15301 tcatttgcta aaacagttaa agctgtttat ggtgttgaag tgattgaagc agctgttaga
    15361 gatgctcaac acaatgccgc tcttaatggc atcaccaatg cctactttgt cgcagatacc
    15421 gcagaacatg ccatggcaac atgggcgaaa gatggcatta aaccaagtgt tattctggta
    15481 gacccgccac gaaaaggctt aacagaaagt ttcatccaag ccagcgtggc aatgggacca
    15541 caaaaaatta cctacgtttc ctgcaaccca gccaccatgg cgcgtgacat caagcgttat
    15601 caagagttgg gttacaaact ggccaaagta cagcccgtag atttgtttcc ccagacgcat
    15661 cacgtggagt gtgtaagttt acttgagaaa cggagttaaa gagtccgcct cactctgtta
    15721 tttcaaggtg agacgctcaa aaggtccact ggaccttttg actcccgcag acccatcacg
    15781 tcgagtgcgt agtgttgcta atcaaggagt aaggcagctg cctccacatg acaaaaataa
    15841 ggagaaacga atgcctatta ccaaacacga gattcccctt ttagaatgtg atagtaatcc
    15901 atcagccgtc attataccta cacatgaagg tctacagcta agtgtgtcta tgctttctta
    15961 gaagacgaga ttgaagcttt tgctttagac catcaaggta aaaaagtagc agaattcatt
    16021 tcagccacaa aggtttaccc tgtttatcag ataccttata aaggccaaga catttgccta
    16081 gctcaagctc ccgttggctc agctcctgcg gcgcaattca tggactggct gattggttat
    16141 ggggttaaag aaatgatcag tacaggaacc tgtggcgttt tggttcctat cgcagaaaac
    16201 cgttttttag tgccagttaa ggctttgcga gatgaaggaa caagttatca ttacgtggca
    16261 ccatcttgtt atattgatat tgatcctaag atgcttagac cgatagagaa aacgctttca
    16321 gcacaggggt tagcttatca agaggtcatc acctggtcaa cagatggttt ttaccgagaa
    16381 accaaagaaa aagtggctta tcgccaggaa gaagggtgtt cggttgttga gatggaatgt
    16441 tctgccttag cagcagtagc tcaattaaga ggcatccttt ggggacaact cttatttaca
    16501 gcagatactc tagcagatgt agaggtttac gatcaacgca accggggcgc tgattccttt
    16561 agttttgcgc ttcatttatg cttggaagtg ttcaatacgc tagaaaagga tggaaaggct
    16621 actgattttt gaaacatgag ctctgccttc tgttcctatt tgtagtataa tgaggcttgt
    16681 attgctttta gactaagctt agaaaagaga aaaacaatgt cagatttaac acaagagatg
    16741 accttaaatg ttattagtat ttttgataat taccaaattg aacttgctga aaaaggacac
    16801 cttattttaa gtactgaagt gactgagacg gctttgaatt actatgggaa tgctcatggt
    16861 ggttatcttt tcactctatg tgatcaagta ggtggtttgg ttgctagaac tactggcgta
    16921 gaatctgtca ccttacaggc caatgctaac tatttaaaag cagggcacaa gggagacaag
    16981 ctgatggttg aaggtcgatt ggttcatgga ggtcgaacca cgcaggtggt tgatgtgacg
    17041 attcataacc aaacaggagc tcttctaaca aaaacaagct tgaccatgtt tgtgactggc
    17101 cgacgtcata acgggtgatt aatgaatcaa taaaggaaaa aagtcccaac tctctgagaa
    17161 ttgggacttt tttcctttat tgagagatac cataaatcac ccgttagacg ggtggttata
    17221 gtttatttac ggtaaagttt gtgatgttgg taaacagggt caaaagtaat atttacccca
    17281 agttttcgga gtacattttt atcttcttcg gttaagataa cggtagaatg ggcttcgcta
    17341 cctttcaaat tgccgagttc tttcatggct agattagctt cttcactagt catagcagta
    17401 atggctaacg caatcagaat ttcattggaa tgaagccgcg gattgtgact gcctaagtgg
    17461 ttcactttga gcccttgaat aggtttcacg tattcgggtt caatcaaatg agtggtttta
    17521 tcaatctttg ccaaggtttt aatcgcattg atgataacag ctgcagttgg tccaaagagt
    17581 tcagatgttt tacctgtcac aatttgccca tttggtagtt ggagagcaag ggcaggttgg
    17641 cctgtttgct ctgctttttg atgagcagct acagtaacgt gacgatcgtc aggggtgaca
    17701 ccgatatcat tcatcaagag ctcaattttt ttgacagcag attctgtgac ccgctctgcc
    17761 ttaaaatcca cgagggtttg gtaataacga cggatgattt cttgtttaga agctttgata
    17821 gcagcttctt cattgacgat agaaaaacca accatgttaa cacccatatc ggttggtgag
    17881 gcataaggag acttgctcaa aatacgctcg aaggtccgat ttaagacagg aaagacctcg
    17941 atatcacggt tgtaattaac ggccgttttg ccataagttt ccaaatggaa ggggtcaatc
    18001 atattcacat catctaggtc tgccgtggct gcctcataag ccagattgac agggtgatga
    18061 agaggaagat tccaaactgg gaaggtttca aatttagcat agccagactt aaccccattg
    18121 atttggtcgt gatacatttg ggacatgcaa gtggctaatt ttccagaccc agggcctggt
    18181 gctgtaacaa cgataaggtt acgactcgtt ttgatgtaat catttttccc catcccctca
    18241 gatgagataa tatgattaat atctgtcggg tagcccttga taggataatg caggtaagaa
    18301 gcaatgccat ttttttcgag ttgcttgcga aaggcatctg cagcgggttg gttattgtat
    18361 tgagtaataa cgacagaacc tacgtaaata tctaacgtgt taaatttgtc aattaagcga
    18421 aagacttctt ggtcataaga aatgcctaaa tcaccacgcg ctttagaatg ttcaatatta
    18481 ttagcgttaa tggcaattac aatttcgact tgttctttga gctctttgag taatttaatc
    18541 ttattgtcag gttcatagcc aggaagtaca cgggcagcat ggtaatcttc taacatttta
    18601 ccaccaaatt ccatgtaaag tttgccatca aattggctga tacgttctaa aatatgatct
    18661 ctttgcaagt ttaaatactt gttagaatca aaagcaatag tcttcatttt ttcacctctg
    18721 taaagcatta taacaaagaa ataccaagaa agaaaaggtt ttttgcaggg atacctcatt
    18781 tagtgacttt ttaaaaaaac ttaggcttgg ttcttagaag aaaaatgtat ccgctatgtt
    18841 caaaagaact tgacaattaa aatgagcttt actatactag taacaattaa atactacttt
    18901 aaacatgttc cgtgaagcat gaaagtctta acagtaaaga gagttttttt gtcatctctt
    18961 attatttagt gaactttgat ggacttgcgg acttgtttcc aagtcttttt ttgacctttg
    19021 ggcaaagggt attcaattaa ttaaggtagt cagtgagcta ctattaagaa ggagacaatt
    19081 atgtcaacat tatcattgga caccacaaat aagcgagctt tagtggcagc tattgcagct
    19141 tcaggaacgg atgacttgaa tgtcatgttt cttgcctttt ccatgtcttc cattatgaca
    19201 gacttgggtc ttagtggcac tcagggtggc tggattgcga ccattaccaa tttaggaatg
    19261 ttggtgggag gcctcttatt cgggttgctg gctgataggc accacaagtt taaggtcttt
    19321 aaatggacga ttttactgtt ttcagtagca acaggattaa tttattttac ccagtctttg
    19381 ccctacctgt acctgatgcg atttattgct ggcataggag ttggaggtga gtacggtgta
    19441 gccattgcca taatggcagg gattgtacca ccagaaaaaa tgggtcggat gtcttcttta
    19501 aatggtattg ctggccagct tggctccatt agttcggctc ttttagcagg gtggctggct
    19561 ccaagtctag gctggcgtgg cctttttctc tttggattac ttccgattct cttggtgatt
    19621 tggatgactt tagccataga tgaccaaaaa atttgggatc attatgggca agaggaggaa
    19681 gaatgcagtc aaccaattaa aatcaatgaa ctattcaaaa caaaatcctt aacagctcaa
    19741 acccttgctt taatggtcat gacgactgtg cagattgcgg gctattttgg aatgatgaat
    19801 tggttgccaa ccattattca aacaagctta aacctttcag taaaaagttc gtccttgtgg
    19861 atggtggcta ctattgtcgg catgtgtttg gggatgctgt gttttggtca gcttttagat
    19921 tgctttggtc cacgtctgat ttattccctc tttttattag catcatctat ttgtgtttac
    19981 ctttttcaat ttgccaattc catggcgagt atggttattg gaggtgctat tgtcggtttt
    20041 tttgtcaatg ggatgtttgc tggctacggg gctatgatta ccagacttta tccccatcac
    20101 attcgatcca cggctaataa tgttattttg aatgttggcc gtgctctagg aggattttcg
    20161 tccgttgcta ttggtagtat tttagatgcc tcaggtattt caatggtcat gattttccta
    20221 gcttctctgt atgtgattag ttttggagcc atgtggtcta ttggtcagtt aaaagcagaa
    20281 cgctaccaac aattgcgtta gcaaagagat gggtgttagt atttgagtta ctttcactca
    20341 caaagctatt ttattgattg agataaccaa aaaggtaagc acttagggat tgcttacctt
    20401 tttggttctt ttcttttgtt ttactccgct actttggcca taaaatgttt actgttcacc
    20461 ctaactctag tatgacaggc ttttccaatg agttgttctc ccacaaaagc ttccaagcgg
    20521 aaatcgtatt ttcgaccttc ttctttaagg gctgtaatga tgattgtaac aggatctcca
    20581 atggctgaag ctgccaaatg ttggatagct atctcgctgc caaccgttgt aaagtcactg
    20641 tcgagctgtt cttgggcaaa taagtaagaa gcattttcca taaaagcaac aagagcagga
    20701 gtagctagga cattcagcgt tcctgagctc atggctttag ccgaatgatt gggacgggtt
    20761 tcataagtgt gagaataaat agtcataagt taatctcctc gtttctataa gcctattata
    20821 atatattaag cgcttacagt catgaaacca ctgttttttt atgttatact taataatgaa
    20881 ttttctttga aagggttatg atgcgtttag ataaattttt agtggcaact ggtgttggaa
    20941 ctaggagcca agtcaaactt ctcttaaaaa agaaggctat cttcgtcaat caaaaggttg
    21001 aaacctctgc caaagcacac attgatgaat acaaggattt ggtgacgtat caaggcacgc
    21061 ccttggtcta tgaatccttt gtttattacc tgttaaacaa gccgtcaggg tatgtgtcgg
    21121 caacccagga cagacagcag gctactgtta tggagttgtt agatgacacg gctaggcaaa
    21181 aaacagtttt tcctgtgggt aggttagata aggatacccg tggacttttg ctcttgacca
    21241 ataatggaca actagcccat gaccttttat cccctaaaaa acatgttacg aaagagtact
    21301 tggctaaagt agtaggcatc atgactgaag ctgataaaga ttattttgct agaggtattt
    21361 ccttaaagga tcaccagtgc ctacctgctc atttagaagt gcttgctagt gatttacagc
    21421 agcaaacaag tttggttaaa attaccatac aagaaggaaa attccatcag gtgaaacgca
    21481 tggtagcagc ctgtggaaag ggagtgcttg acttgcagcg tctcagcatg ggaccattaa
    21541 aactagaccc tagccttgca gaaggggagt ttaggcgcct gacgcctgag gaattgcaaa
    21601 gcctggcacc ttattgtcag agctcactat aaaaagaaac ctcccctgtc agtactgacc
    21661 ccaaaaagtt ggacaaataa ttattgaaag g
//
LOCUS       DAUGFP010000026        21534 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.026, whole genome shotgun sequence.
ACCESSION   DAUGFP010000026 DAUGFP010000000
VERSION     DAUGFP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21534
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.026"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..675
                     /locus_tag="VH663_001025"
     CDS             <1..675
                     /locus_tag="VH663_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEQ8174234.1"
                     /translation="RREQQAYMTNLETYGKHLEKLYRDVRAFQSDYLSRIERLGQAIK
                     SESITQIQDIYAQTVHEANDYWDDKHYNISKLRKINISSIKSLLSAKIISAEKSGIDL
                     NVEVPDNIKETYIPELDLLLLMSIFCDNAIEAALEAQQPHMSIAYFLLDDYQMFVVTN
                     TTKKKVDINKIFEEGYSSKGSERGIGLSNAQRILKKYPYLSLRTKSFDKEFSQTLTMP
                     KEEVDR"
     gene            672..1955
                     /locus_tag="VH663_001026"
     CDS             672..1955
                     /locus_tag="VH663_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8174235.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSVIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFIYLAIFIWVIVKLDRFAKDQLSQKLTQ
                     AQNERIAYLENYNQSIEQLYREIRTVKHDSENILISLKDSIDSGDIDLITRVYDTVIQ
                     QSATSMMRTNYEISSLDNIKEAVIRSIMNSKLLEAQYLGIELYIEIPDVIDHLPIKLI
                     DLIVLFTGLVDNAIETAKGSRRPFLSIAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQ
                     QQNSAHFLTVLDSYPQITLSTKSDHYRLRQLLEMR"
     gene            1959..2699
                     /locus_tag="VH663_001027"
     CDS             1959..2699
                     /locus_tag="VH663_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8174236.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            3239..3598
                     /gene="rnpA"
                     /locus_tag="VH663_001028"
     CDS             3239..3598
                     /gene="rnpA"
                     /locus_tag="VH663_001028"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEQ8174237.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            3564..4391
                     /locus_tag="VH663_001029"
     CDS             3564..4391
                     /locus_tag="VH663_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ8174238.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLILMPLFNMQIKSSQKMQDIQPELRELQRKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            4403..5317
                     /locus_tag="VH663_001030"
     CDS             4403..5317
                     /locus_tag="VH663_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEQ8174239.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLPRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINRQNAPAVNSADVEPEEIKATQRLEAED
                     TKVVPLMSEDSPAQIPSNLAETVTETKAQQPSIPVEESEVPQDAGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            5631..5765
                     /gene="rpmH"
                     /locus_tag="VH663_001031"
     CDS             5631..5765
                     /gene="rpmH"
                     /locus_tag="VH663_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEQ8174240.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            6039..6743
                     /locus_tag="VH663_001032"
     CDS             6039..6743
                     /locus_tag="VH663_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEQ8174241.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMAEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
     gene            6792..8111
                     /locus_tag="VH663_001033"
     CDS             6792..8111
                     /locus_tag="VH663_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ8174242.1"
                     /translation="MNMKKLASLVMLGASVLGLAACGGKSQKEAGASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTKEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKTFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAISNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            8215..9102
                     /locus_tag="VH663_001034"
     CDS             8215..9102
                     /locus_tag="VH663_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ8174243.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLMNTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIVILLTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            9115..9945
                     /locus_tag="VH663_001035"
     CDS             9115..9945
                     /locus_tag="VH663_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ8174244.1"
                     /translation="MTKKKLTASDILTTVMLCVLTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            9965..10420
                     /locus_tag="VH663_001036"
     CDS             9965..10420
                     /locus_tag="VH663_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEQ8174245.1"
                     /translation="MFYDRLKNISRYRGIHPHLDAAITFLQTTDLRQLAEGKYPILGE
                     KVFAVIQRNQLSKADNSLLEYHKRYADCHLLLAGNECIRYGIGNQAEVVPFEQEADIG
                     FVTCDRTYDLDLVDDSFAYFFPNEPHQPNNFNQAGEAVTKCLIKVLMAD"
     gene            10464..11126
                     /locus_tag="VH663_001037"
     CDS             10464..11126
                     /locus_tag="VH663_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEQ8174246.1"
                     /translation="MTSKKQGLLHSLFNLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLVTTYSSRFKHYFKSGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVRKELSLSTLFKRSFLLAGLFFP
                     WSFAFLALICLTIFSLQLSLLTLFGGVALLAIIGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            11138..12052
                     /locus_tag="VH663_001038"
     CDS             11138..12052
                     /locus_tag="VH663_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEQ8174247.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGAISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYSVADYWNAISEAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDGHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIADKDLEKAKALQYTINEIISVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLSELVEEDRVICQRAAALINQAKETF"
     gene            12074..13012
                     /locus_tag="VH663_001039"
     CDS             12074..13012
                     /locus_tag="VH663_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ8174248.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMAQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITLEKIENNLASS"
     gene            complement(13123..13953)
                     /locus_tag="VH663_001040"
     CDS             complement(13123..13953)
                     /locus_tag="VH663_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEQ8174249.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEELLNLAKLIDASERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
                     "
     gene            14238..15029
                     /locus_tag="VH663_001041"
     CDS             14238..15029
                     /locus_tag="VH663_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEQ8174250.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHEAEELALAQEMGVAYHNVV
                     GFDQATISGALALADQYPHVYATIGWHPTEAGSYSEAVEESIVSQLSHPKVIALGEIG
                     LDYHWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKETTANAKRVFKLD"
     gene            15001..15591
                     /gene="rnmV"
                     /locus_tag="VH663_001042"
     CDS             15001..15591
                     /gene="rnmV"
                     /locus_tag="VH663_001042"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEQ8174251.1"
                     /translation="MQRGYSSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHARFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            15705..16577
                     /gene="rsmA"
                     /locus_tag="VH663_001043"
     CDS             15705..16577
                     /gene="rsmA"
                     /locus_tag="VH663_001043"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEQ8174252.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVED
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            17003..17875
                     /gene="rsgA"
                     /locus_tag="VH663_001044"
     CDS             17003..17875
                     /gene="rsgA"
                     /locus_tag="VH663_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEQ8174253.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            17885..18547
                     /locus_tag="VH663_001045"
     CDS             17885..18547
                     /locus_tag="VH663_001045"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEQ8174254.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGADIMTIHTESTRHIHGAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            18540..19172
                     /locus_tag="VH663_001046"
     CDS             18540..19172
                     /locus_tag="VH663_001046"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEQ8174255.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVPQKAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMLTYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            19174..20445
                     /locus_tag="VH663_001047"
     CDS             19174..20445
                     /locus_tag="VH663_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEQ8174256.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     MTYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEEINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLNASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQATKSLLNMPLLEEENNEN"
     gene            20435..21373
                     /locus_tag="VH663_001048"
     CDS             20435..21373
                     /locus_tag="VH663_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEQ8174257.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVEEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
BASE COUNT         6425 a         3856 c         4685 g         6568 t
ORIGIN      
        1 agaagagaac aacaagctta tatgactaac ttagagactt atgggaaaca tcttgagaaa
       61 ctctatcgag atgtaagagc ttttcaaagt gattacctga gccgtataga gcgtttaggt
      121 caagccatta agagtgaatc cattactcag attcaagata tttatgccca aacggttcat
      181 gaagctaacg actactggga tgataaacac tataatatct cgaagcttag gaaaattaat
      241 atctcatcga ttaagagctt attgtcagct aaaattatca gtgcggaaaa atcaggtatt
      301 gatttgaatg tggaagtgcc agataatata aaagagactt acattcctga gttggactta
      361 ctattgttga tgtctatttt ttgtgataac gccattgagg cagctcttga agcacagcaa
      421 cctcatatgt caattgctta ctttttgtta gatgactatc agatgtttgt tgtgacaaat
      481 acgactaaaa aaaaggtaga cattaacaag atctttgaag aaggctattc ttcaaaaggt
      541 tcagaacgtg gtatcggcct ttctaatgcg cagcgtattc tcaaaaagta tccttattta
      601 tccttgcgga ctaaaagttt tgataaggaa tttagccaaa ccttgaccat gccaaaggag
      661 gaggtggata gatgatggtt atatacagta tggctttatt ctatgctacc atctttatta
      721 acagctgggt catttttgca aaggtttctg ctattaaact aagctggaaa cgggttagcg
      781 ttataggcat tagttttgta attgctaata tgatatttga caaagtcatt ttgattgacc
      841 aactgttttt tattattgtt agtttgttaa gtgctccgaa aaagaaatta tttgagcata
      901 tgtttaatgg tttctttact atcttgattg tcgaattact gtttcgcgtc atcggatctt
      961 tctttttacc agctgtctta ggatttagca ttggtcaaat taataataac cttaagcttt
     1021 tagagttatg ttatcttttt gtattaccaa tattttatct gtttagctac attttcagta
     1081 ttgacttaag cttaataagg tttatcagtg aagataagat gaaaaagtgg gtattttgga
     1141 tgaatactgc catgttttct tactactttt tcgcccattt tttggtaact gtgcaaagcg
     1201 gatttcttgc actatatttc cagtaccggt ctattttagt atttatttat ttagctattt
     1261 ttatttgggt tattgtgaaa ttagatcgat ttgctaaaga tcagctgtca caaaaattaa
     1321 cacaagcaca aaatgaacgt attgcgtatc ttgaaaatta caatcaatcg attgagcaac
     1381 tttaccgaga aatcagaaca gttaaacatg attctgaaaa tattttaatt agtttaaaag
     1441 atagtatcga tagtggtgat atagatctga ttactagagt ttatgacacg gttattcagc
     1501 aatcggcaac ttctatgatg cgcacaaatt atgaaatatc ttctttagac aatatcaaag
     1561 aggctgtgat tcgcagcatc atgaattcca aattgcttga agctcagtat ttgggtattg
     1621 aactttatat tgagattcca gatgttatcg atcatttacc tattaagtta atcgatttaa
     1681 ttgtactttt tactggacta gtggacaatg ctattgagac agccaaaggc agtagacgtc
     1741 cttttttatc tattgcatac tttaaacaag ataacaagca attatttatt attgaaaata
     1801 gcacaaaaac caatcgtgtg gatatagcca aacgctttga tgctcagcag cagaactcag
     1861 cccacttttt gactgtttta gatagttacc cccaaataac gctttcgact aaaagtgacc
     1921 attatcgatt aagacaattg ttagaaatga gataaagtat gatgaacatt tttattcttg
     1981 aggatgattt tatccagcag acacgcatag aatctatcgt agtaggcatc ttaaaagaga
     2041 ctagaatacc atgtaatcag cttgaagtgt tctcgactcc tcaaaaatta tttgagagta
     2101 tccaagaaag aggagaccat cagctgtatt ttttagatat tgaaattggt gaatatactc
     2161 gttgtgggct tgaattagct gcagctattc gacaaaaaga tcctaatgcc gttattgtgt
     2221 ttgtcaccac tcattcagaa tttgctccta tttcttttaa atataaggta tccgctctag
     2281 attttattga taaagcaggt ggtcaaaaac agtttaaaga acaaatcgaa gaatgtatcc
     2341 gatatactta cgatatgatg tctagtcgtg agtcaaagga catgttttta tttgagactc
     2401 cacagacaag attaaaattg ccctataagg atattttata ctttgctaca gccacaactc
     2461 ctcacaaagt ttgtttatgg actcagacag aaagattaga gttttatggt aatctatctg
     2521 agattcaagc agtagcaccg aaactttttt tgtgccatag atcctacctt gtgaatctag
     2581 ataaggttgt tcgaattgat aagagtaagc agctccttta tttcgaaaat ggtgattctt
     2641 gtatggtatc acggttaaaa atgaaatgtc tgtttgaacg gtgggaggcc cttcgctaaa
     2701 gttaagagag gaataacatt ttaggacgaa aaagcaacat ttcaggaaat gatgctctta
     2761 ttgaagtatt tttcgctata ataattaatg taaataaaga tttacgaagt catgagttta
     2821 tcgagcaatc aatccccaat tcaatcaaaa tattgttttc tctctctcca taaacaaata
     2881 gtatttttga tatgatggct cggcagacaa aatgacgatg tcagttgtct ttgtttggat
     2941 atcatattag ctgattgtaa aatcagtatt aaagcccggc ttgccgggtt ttttgatagg
     3001 taaatacagg gataagtagc tgacgtcttg tggattctga ctttttatgg ttgttttctg
     3061 aaggttattc ctttgtcgaa agaagaacta tactacgaaa agatggagtg acaagaggca
     3121 gttgaagcac aaaaactaga aatagtacag aaagaggctt aatctgaaag ggttagggct
     3181 ttttaatttt ttaggggatg tggtataatg gtaataattt agatattgga gttaaatttt
     3241 gaagaagacc tatcgtgtca agcgtgagaa agatttccaa gccatattta aggatggaaa
     3301 aagtacagca aatcgaaaat ttgtcattta tcatttaaat agaggccaag accacttccg
     3361 tgtgggtatt tctgtcggta aaaaaatagg aaatgcagtc accagaaatg cagtcaaacg
     3421 aaagatacgt catgttatca tggcattagg gcatcagctg aagtcagagg atttcgtggt
     3481 gattgcccgt aagggtgtcg agtctttgga gtatcaagag cttcaacaaa atttacatca
     3541 tgttttaaag ttagcacaat tgcttgagaa aggttttgag agtgaagaaa aacattaaaa
     3601 ttgcaagaat agtcccctta gttctattat tagtggcctg tggtcgtggt gaggtaacgg
     3661 cacaatcatc tagtggttgg gaccagttgg tttacttatt tgccagagca attcaatggc
     3721 tttcctttga tggttcaatt ggtgttggca ttattctttt tacccttact atccgtctca
     3781 tactcatgcc tttgtttaac atgcaaatca aatcaagcca gaagatgcaa gacatccaac
     3841 ctgagcttag agaattgcaa aggaaatacg ctggtaaaga cacacaaacg cggatgaagt
     3901 tggctgaaga aagtcaagcc ctttacaaaa aatatggggt taacccttat gctagtctct
     3961 tacccctctt gattcagatg ccagttatga ttgccctatt ccaagccttg acacgggtat
     4021 ctttcttaaa aacagggact ttcttgtggg tggaattggc acaacatgat catttgtacc
     4081 ttttaccagt tttggcagct gtcttcactt tcttgtccac ttggttgacc aatctagcgg
     4141 ctaaagaaaa aaatgtcatg atgactgtta tgatttatgt gatgccgcta atgatctttt
     4201 tcatgggctt taacttggct agtggagtag tgctctattg gacggtctcc aatgcctttc
     4261 aagtggtaca actactgtta ttaaacaatc cttttaagat tattgcggaa agacaacgtc
     4321 ttgccaatga agaaaaagaa cgccgtcttc gtgaacgtcg tgctcgtaaa aaagcaatga
     4381 agagaaaata ggagtttagg ttatggtatt atttacagga aaaacagtcg aagaagctat
     4441 cgaaacagga cttcaagagt tagggttacc acgccttaaa gcgcatatca aagtcatttc
     4501 aaaagagaaa aaaggtttcc tcgggtttgg taaaaagcca gcccaagttg atatcgaagg
     4561 tatcagcgac aagacggttt ataaggctga taaaaaagcc actcgaggtg ttccagagga
     4621 tattaatcgt caaaatgctc ccgcggtcaa ttctgctgat gtagaacctg aagaaataaa
     4681 agccactcaa aggttagaag cagaagatac caaagtggtt cctctcatgt ctgaagatag
     4741 tccagcgcaa atcccttcta atcttgctga gacagtcact gaaacaaaag cacaacaacc
     4801 gtcaatccca gtcgaagaat cagaagtgcc ccaagatgct ggtaatgatg gtttcagcaa
     4861 agatattgaa aaagctgctc aggaagtgtc cgattatgtg actaaaatta tctatgaaat
     4921 ggatatcgaa gctaccgttg aaaccagcaa caatcgtcgc caaatcaatt tgcagattga
     4981 aacgccagag gcgggacgtg tgattggtta ccatggtaag gtcttgaaat ccttgcagtt
     5041 gttagctcaa aatttcttgc acgaccgcta ttcaaaaaat ttttcagtat ccttgaatgt
     5101 ccatgattat gtggaacatc gaacagaaac cttgattgac tttacgcaaa aagttgccaa
     5161 acgcgttttg gaatctggtc aagattacac catggatccc atgagtaaca gtgagcgtaa
     5221 aatcgttcat aaaaccgttt catccattga aggggttgat agttattctg aaggcaatga
     5281 ccctaaccgc tatgtggtag tcagtcttca acgttagatc ctaaaagaat ctttggtagt
     5341 tagtttagct atcgaagatt ttttgtatgg ccagcttaac cgtttgtttt actgatctca
     5401 ttgattattg ttgctattga taaggtttca acggtaaggc aaaggaacag tgtcaaagaa
     5461 gagacacttt atgaaatgag cggcctctta aatgagagag aaactgttga aaaacgaaaa
     5521 taaatcttga cagtagaccc tctttaagat agaatattat agtatgttta gtaactgata
     5581 tcacaaatag ccggctaaac gaatacaaaa tctatgagga ggtattcact gtgaaacgta
     5641 cttatcaacc aagtaaaatc cgtcgtcaac gtaaacacgg attccgtcac cgtatgtcaa
     5701 ctaaaaacgg acgtcgcgtg cttgctgcac gtcgtcgtaa aggacgtaaa gtattatcag
     5761 cttaattgca aaaaatacaa ctaaagtaaa ccaagagtgc tcgaggctct tggtttttta
     5821 acgttttcta atcggaaatt tgcaatgtat aagtatggtg tcaactcctt acctttttat
     5881 ttgttgtatg aaagagcatt ccaaataata gaaaataata taaaaaatga aagtagattc
     5941 taaaagtctc attgctattg aaagcgcttt attttttgtt ataataactt tgtgaacgtt
     6001 atttttatta aaactaagaa acacaaggag tagaaactat gcctgataaa ccaaccaaag
     6061 aaaagctcat ggagcagtta aagggtggga ttatcgtttc ttgtcaggct ttgccaggtg
     6121 agcctctcta ttcagagact gggggtatta tgccactgct ggcaaaagct gctcaagaag
     6181 ctggtgctgt cggcatcaga gctaattcgg ttagagacat taaagaaatt caagccataa
     6241 cagacctacc aattatcggt atcatcaaaa aagattaccc gccgcaagag ccttttatta
     6301 cggcaacgat ggccgaagtg gatcaattag ctgccttaaa tattgcagtg attgctatgg
     6361 attgtaccaa gcgtgaccga catgatggct tggatattgc tagctttatt cgacaagtta
     6421 aggaaaagta ccccaatcag cttttgatgg cagatattag tacctttgat gaaggactag
     6481 tggcccatca ggctggcatt gattttgttg gaaccacctt gtcaggctac accccgtata
     6541 gccgtcagga agcaggtcct gatgtggcat tgatagaagc tctttgcaaa gcaggcattg
     6601 ctgtcattgc cgaaggaaag attcattccc cagaagaagc caagaaaatt aatgacttag
     6661 gtgtggcagg tattgttgtt ggaggggcca ttacaaggcc taaggaaatt gcagagcgtt
     6721 ttatcgaagc gctcaaatcc taatagtggt tgtcaaacta gaaacacaca tcaaaaaaag
     6781 gagattttac gatgaacatg aagaagttag cttcattagt gatgcttggt gcatctgttt
     6841 taggactagc agcttgtggc ggaaagagcc agaaagaggc tggtgcaagt aaatctgata
     6901 ctgctaagac agaaatcaca tggtgggctt tcccggtctt cacgcaagaa aaagctgaag
     6961 atggtgtggg aacctatgag aagaaattga ttgctgcctt tgagaaagct aatccagaaa
     7021 tcaaggtaaa attggaaacc attgatttta catctggtcc tgagaaaatc acaactgcta
     7081 ttgaagctgg aacagctcct gatgtcctct ttgatgctcc aggtcgtatc attcaatatg
     7141 gtaaaaatgg gaaattggct gacttgaatg acttgttcac aaaagagttt accaaagatg
     7201 ttaacaatga caaattgatt caagcctcaa aagcaggaga tacagcttac atgtatccta
     7261 tcagctctgc tccattctac atggcgttga ataaaaaaat gctcaaagat gctggcgtcc
     7321 ttgaccttgt gaaagagggg tggacaacag atgattttga aaaagtcttg aaagcattga
     7381 aggacaaagg atataaccca ggttctttct ttgctaatgg tcaaggtggt gaccaaggac
     7441 cacgtgcctt ctttgctaac ctttatagta gtcatattac cgatgacaaa gtaaccaaat
     7501 acaccacaga tgatgctaac tctattaaag ccatgactaa aatttccaat tggattaaag
     7561 atggtttaat gatgaatggc tctcaatacg acggttcagc tgatattcaa aactttgcga
     7621 atggccaaac ttcctttacc attctttggg cgcctgcaca acccggcatc caagctaaat
     7681 tgttagaagc tagtaaagtg gattaccttg aaatcccatt cccatcagat gatggcaaac
     7741 cagaactaga atacctcgta aatggttttg cggtctttaa taacaaagat gaacaaaaag
     7801 tcgctgcctc taagacattt atccaattta tcgctgatga taaagaatgg ggacctaaaa
     7861 atgttgttcg tacaggtgct ttccctgtaa gaacttctta tggggatctt tacaaagaca
     7921 aacgaatgga aaaaattgct gaatggacaa aattctactc accatactac aacacgattg
     7981 atgggtttgc tgaaatgaga actctttggt tcccaatggt tcaagccatc tctaatggtg
     8041 atgaaaagcc agaagatgct ttgaaagcct tcactgaaaa agcaaacaag acaatcaaaa
     8101 aaacacaata agtgttcaga acaagtggct tcttttcacg gcacatgtca aatggtcact
     8161 ggaaaggagc ctttcctttt ttaggagaga ttaatagaaa aagaggtatt tcaagtgaac
     8221 gtcaataaat taaaaatgag agagacgctc atctcatacg ctttcttagc tccggtcttg
     8281 gttttctttg tgatttttgt cttgataccg atgattatgg gctttgtgac gagttttttc
     8341 aattattcca tgacggattt tacctttgtt ggttttgcta attatgctag gatgtttcaa
     8401 gatcccattt tcatgaagtc tcttatgaac accttgatta ttgttattgg ttcggtacct
     8461 gttgtagttt tcttttccct ctttgtggcg gctaaaacct acgacaaaaa tgtagtggcg
     8521 cgttcgtttt accgggcagt tttctttctg cctgttgtta ccggaagtgt tgcggtgacg
     8581 gtggtttgga aatggatcta tgatccgatg tcagggattt tgaattatgt cttaaaatat
     8641 gctcatgtga ttgaacaaaa cattagttgg ctgggagata agcactgggc tttgttagct
     8701 attattgtta ttttattaac cacatctgtt gggcagccta ttattctata tattgcagct
     8761 atgggaaata ttgataactc tttggtagaa gctgcgcgtg tggatggggc gacggaattt
     8821 caggtgtttt ggaatatcaa gtggccaagt ctcctgccaa caaccttata tattgctgtc
     8881 ataacaacca ttaattcctt ccaatgtttt gctttgattc aactcttgac gtctggaggg
     8941 ccaaattatt caaccagtac cctcatgtat tatttgtatg aaaaggcctt taagctctct
     9001 gaatatggct atgccaacac aatgggtgta ttcttggcag tcatgattgc aatcatcagc
     9061 tttgctcaat ttaaaatttt aggtaatgat gtggaatatt aggaggatat taagatgacg
     9121 aaaaagaaac taaccgcatc agatatctta accactgtga tgctatgtgt tttgaccatt
     9181 ttgtttattt ttccatttta ttggattatg acaggagcat ttaaggctca agccgatacc
     9241 attatgattc caccgcaatg gtggccaaag gcgcctacta ttgaaaattt taaggccttg
     9301 gtagtgcaaa atccagcctt aaaatggttg tggaatagtg tctttatttc ggtggcgacc
     9361 atgttcttgg tttgtggcac ttcctctttg gctggctatg ctttggccaa aaaacggttt
     9421 tatgggcagc ggcttttgtt ttcgattttt attgctgcta tggctttgcc aaaacaggtt
     9481 gtcctagtac ctcttgttcg gattgttaat tttatgggaa tccatgatac tttggcggct
     9541 gttattttgc ctcttgtggg ctggccattt ggtgttttcc ttatgaaaca gttctcagaa
     9601 aatattccaa ccgagttatt ggaatccgcc aagattgatg gatgtggcga aattcgtacc
     9661 tttttcaatg tggctttccc tattgtcaaa ccaggctttg cagcgctagc gatttttacc
     9721 tttatcaata cttggaatga ttattttatg cagttagtga tgttaacctc tcgggaaaat
     9781 ttaacgattt cacttggggt tgccactatg caagctgaaa tggctactaa ttatggcttg
     9841 attatggcag gggctgccat ggctgcagtg cctattgtaa cagtctttct tgtcttccaa
     9901 aaatcattta cccaaggcat tactatgggt gctgtgaaag gttaaatcaa gaaaggtttc
     9961 taacatgttt tatgatcgtt taaaaaatat tagccgttac cgagggattc accctcattt
    10021 ggatgcggcg attacctttt tacaaaccac agatttgagg caactagctg aagggaaata
    10081 tcctatttta ggggagaaag tatttgcagt tattcaaaga aatcaattga gtaaagcgga
    10141 caattcattg ttggaatacc acaaacggta tgcagattgc caccttttgc tagctgggaa
    10201 tgagtgcatt cgttatggta taggtaatca agctgaagta gtgccttttg aacaagaagc
    10261 tgatattggc tttgtaacct gtgatcgaac ctatgaccta gatttggttg atgatagctt
    10321 tgcttacttt tttcctaatg agcctcatca acccaataat tttaaccaag caggggaagc
    10381 agttacaaaa tgcttaatca aagtgttaat ggcagattag gttgatttgg cagtgaacca
    10441 attgatcgaa aggaagtgat ggtgtgacgt cgaaaaaaca gggactttta cattctcttt
    10501 ttaatcttga tagtaaatgg atgagggcta gtgcagcact atttgatttg ctcgtgttta
    10561 atctcttatt tgtcttgtcc tgccttcctc ttctgacaat tggggttgct aaaatggctt
    10621 tgtatgcgag tttgttggat tggcgagaag gtcaagttag tcagcttgtt actacttata
    10681 gctctcgttt taaacattat tttaaaagtg ggatgcgcct aggtcttatt gagcttttga
    10741 taatgagtat ttgtgtctta gatcttttct taattcgaaa ccaatcaggc ctagtttttc
    10801 aagggtttaa agtactttgc attgctgttt tatttctggt agttatactc tttttgtacg
    10861 cttatcctca ggccgtcaga aaagagcttt ccctatctac gctgtttaag cgaagctttt
    10921 tactagccgg actctttttt ccatggagtt ttgctttctt ggctcttatt tgtctcacga
    10981 tatttagcct acaattgtct ctgttaacgt tatttggagg cgtggctttg ctagctatca
    11041 tcggcatcag tagcctaact tacctctact tgattatcat ggaatccctt cttaggcgat
    11101 tcccacttaa taatgacatt gaataaagga cattactatg acagatctta cgaaatacca
    11161 aggcattatt cctgcctttt atgcttgtta cgatgaccaa ggagctatta gccctgagcg
    11221 tgtgcgtgcc ttgacccagt actatattga caaaggggtt caggggcttt acattaatgg
    11281 ctcttctgga gagtgtattt accagagcgt ttttgaccgt caattggtgt tagaaaatgt
    11341 catggcagtc gctaagggca agctgacgat tattaatcac gttgcctgca acaacactaa
    11401 agatagtatt gaattggcag ctcattcaga acgtttgggc gtggatgcca ttgcagctat
    11461 tccgcctatt tatttccggt taccagaata ttcagttgct gattattgga atgctatttc
    11521 ggaggcagct cctcacacag attttatcat ctataatatt cctcaactgg caggggttgc
    11581 cttgacgcca agcctttata agactatgtt ggctaataag cgcgtgattg gggttaaaaa
    11641 ctcatccatg ccagtgcagg atattcaaac cttctgtgcg attggtgggg acggccatat
    11701 tgtttttaat ggcccggatg aacaatttct aggagggcgc ttgatggggg ctgctgctgg
    11761 tattggtggt acctacgggg ctatgccaga attattttta aggctgaacc aacttattgc
    11821 tgacaaggat ttagaaaagg ccaaggccct tcaatatact attaatgaga ttattagtgt
    11881 ccttgtttct gctcatggca atatgtatgg ggttattaag gaagtacttc gtattaatga
    11941 gggtcttgat attggttctg ttcgttcccc attatctgag ttagttgagg aagatagggt
    12001 tatttgccaa cgggctgctg ctctgattaa tcaggctaag gaaacctttt gataagatgc
    12061 gaggggataa caaatgaagc attatttagc aattgatatt ggtggtactg ctatcaagta
    12121 tggcttgatt tctgaaacag gagacctgtt ggaaaaagag gaaatggcca ctgaggctta
    12181 taaaggtggt ccctctattt tggaaaaggt caagggcttg gtaaagacct atcaagacca
    12241 aatggatctg gctggagtgg ccatttcttc tgcaggaatg gttaatcctg ataagggaga
    12301 aatcttttat gccggccctc agattcctaa ctatgctgga actcagttta aaaaagagat
    12361 tgaagagact ttcgggttac cgtgtgaagt ggaaaacgat gttaactgtg ctggtctagc
    12421 cgaagctatt tctggtagtg ccaaggatta tcctgtggct ctttgcttga caattgggac
    12481 gggaatcggt ggctgtttat tgtttaattc ccaagttttt catggtagta gtcattcagc
    12541 ttgcgaagtt ggttaccttc atttgtcaga tggtcagttt caggatctgg catctaccac
    12601 agctcttgtt caagaggtgg ccttagctta tggcgatgac attagccaat gggatggtcg
    12661 tcgtattttt gagcaggcta aagcgggtga tgcgatttgt atagcagcca ttagtaagca
    12721 ggtggattac ttaggacaag gtattgctaa tatctgctac gttgttaacc caaatgtggt
    12781 ggtgcttggt ggcggtatca tggcgcaaaa agattattta gctgataaat taaagacagc
    12841 tcttgatagc tacttggttt ctagtcttgc caagaaaact cagctgaagt ttgctagtca
    12901 tggaaataat gctggtatat taggagctta ctaccatttt aaacaaaaga atgagcgatc
    12961 ctgttcattt ataactcttg agaaaatcga aaataattta gcgagtagct aatcttagtg
    13021 gctgtgagtc tatatgacgt gaagagttct tgtttaagta tactggggga ggtgaatgac
    13081 gaccctttaa aaagaaaaca acgccctttt tgctattgta gtctaaaggt cgttattttc
    13141 tttgtcaata atagtgtttt tgaagatttt ttctttgtgc ggtttgtcaa tcgctaaaac
    13201 gtaagcataa ataatgtcca tcataataag aagaggaaat tgtggtgaca cacgattccc
    13261 ataatgtagt tggtgggttg atgatacggg aataatatct aaggagtcgt caaattcggt
    13321 ttgattatct gttgttaaga ggacagtttt ggcccctcgc tgactagctt gatgaagtgc
    13381 tgtgatgaca gagtttgttt tgccagataa agaaaaacca aaaacaaggc aattttcatt
    13441 gacgaggcta tttgcccaag taaaaccatc agtgtctgaa taagcatcac agattaaacc
    13501 aagtcgcata aagcgtagct tcatttctct tgctactagt ccagaagatc ctttgccaaa
    13561 gaaatagact cgctcagaag catcgatcag cttggctaag ttcagtaatt cttcctcatt
    13621 aacgagctca taggttttat taataagagc atcgtagtcc atcagcacgc gctttgtcaa
    13681 ttctaagtga atagattgga aagtctcttg tgattcttga agactgtgaa ggtaatcaaa
    13741 ggcaaaagca cgataaccag taaaaccaca cttttttgca aatcgagtca aggcggcttg
    13801 agagatgtgt agtcgcttaa ctatctcgga tgcggtcaac tcttgaggtg tcagatcggt
    13861 tgtaataaaa aaatgagcaa tccctttttc taaactggtc atatgttcca atgatgcttc
    13921 gattttggta attaggcggt ggtttttagg catagcaaag cacactcctt acagtttaac
    13981 tatctactag tatagcatag atcgctaaga gaaggtagcg accctttttg tgatacagat
    14041 ttgttcaacc cactttattt gagtaggttg tagtattttt gagggcttat gtaggagccg
    14101 actattaaag gtcatttgtc aaaaaagtag aaaatagaca agtgtcagct agcaaagggt
    14161 tttacgatca acctaaacgg cagaagttct caccaagttt tctgatgtgg ttaagatctt
    14221 tatgttataa taacatcatg acaaataaag tagaaaaact aactattttt gatacccata
    14281 cgcacctgaa tgtagcagaa tttcaaggac atgaagctga agaattggct ttggctcaag
    14341 aaatgggcgt ggcctaccat aatgtggttg gctttgacca ggccactatt agtggagcac
    14401 ttgctctcgc agaccagtat cctcacgtgt atgctactat tggatggcac ccgacagaag
    14461 caggctctta ttctgaagca gttgaggagt ctatcgtctc tcaattatca catcccaaag
    14521 tgattgccct gggtgaaatt ggcttagact atcattggat ggaggacccc aaagaggtgc
    14581 aaatagaggt ttttaagcgc caaatgcaat tagcaaaaga ccatgatttg ccttttgtgg
    14641 tgcacacccg tgatgccttg gaagatacct atgaagtcat caaagcagct ggtgttggcc
    14701 ctcgtggagg catcatgcat tcttactcag ggtcattaga gatggctgaa cgtttcattg
    14761 aacttggcat gatgatttcc ttttcaggtg tggtcacttt caaaaaggct cttgatattc
    14821 aagaggcagc gcagcacttg cctttggata agattttggt ggaaacagat gccccttatc
    14881 ttacaccagt tccaaaacgt ggcaaacaaa atcatacagc ttatacccgc tatgtggttg
    14941 ataaaatcgc agagctacgg gggatgactg tggaagaagt tgcaaaagaa acgactgcta
    15001 atgcaaagag ggtattcaag cttgactgaa aaaattaata ttcaagaggt tcttgttgta
    15061 gaaggcaagg acgacacggc taaccttcgc cgtttttacg aggtggatac ctatgagacc
    15121 agaggctctg ctatcactga agaagattta gaacggatca accgcctaaa tgacttgcgt
    15181 ggtgtcattg ttttgacaga cccagattat aatggtgagc gcattcgtaa gctcattatg
    15241 gcagctgtgc caacagctcg tcacgccttt ttaaaccgaa acgaggcggt tccgagttct
    15301 aagagtaagg gtcgttcatt aggggttgaa catgccagat ttgaggatct ccaaaaggca
    15361 cttgctcatg tcactcagca atatgatgat gagtcttatt ttgacattcg tcaaacagac
    15421 ctgattcgtc tagggctttt aatggcgtca gatagccgta aacgcaggga atacttgggt
    15481 gaaaagctcc gcatcggtta tgccaacggc aaacaactgc tcaagcgcct cgaattattt
    15541 ggcattaccc tagcagaagt ggaagaagtg atggagacat atgaggagta aagagtacaa
    15601 taaaatataa cagccccatt aactgaatgg cgtgtcctgg caaaaaagtt taggctagtg
    15661 gttcaaatgc caaaaagaaa gtgatcataa aaagaagaga atatatgaga attgcagatt
    15721 atagcgtgac caaggctgtc cttgatcgtc acggatttac ctttaagaaa tcttttggtc
    15781 agaacttttt gaccgatacc aatattttac agaaaatcgt tgacacggct gagattgatc
    15841 agaatgtcaa tgttatcgag attggtcctg gaattggggc tttgacggaa tttttggcag
    15901 aaaatgctgc tgaagtgatg gcttttgaga ttgatgaccg cttggtgccg attttggcag
    15961 ataccttgcg cgattttgat aatgtgcaag tggtcaatca agatattttg aaagctgatt
    16021 tgcagaccca aatcaagcag tttaaaaatc ctgatttgcc tatcaaggtg gtggctaacc
    16081 taccttacta catcacaacg cctattctca tgcacttgat tgagagcaaa attccattcc
    16141 aagagtttgt agtcatgatg cagcgtgaag tggcagatcg tatttctgcg gagccaaaca
    16201 ctaaagctta tggttccttg tccattgcgg tacagtatta catgactgcc aaggttgcct
    16261 tcattgtgcc gcgtaccgtc tttgtacctg caccaaatgt cgatagtgct atcctcaaaa
    16321 tggtgcgtcg cgaccagcct ttgattgagg ttgaggacga agatttcttt ttcagagtgt
    16381 ctcgtctcag ttttgttcat cgtcgcaaga ccctgtggaa taacttgact agccattttg
    16441 gcaagtctga agacatcaag gctaaacttg aaaaaggtct ggcactagca gatatcaagc
    16501 cttctattcg tggtgaggcc ttgtcgattc aagattttgg caaattagct gatgctttaa
    16561 aagaagttgg attataaaag ctttcccccg gtgacaataa aaaaattccc ttgtagacaa
    16621 aacagagatg ttcagttcag ggggattttt cttattgaag ctggccgtta aggcatcgta
    16681 gtgcagagct agttttttta atacctcgga aaaaaagggg caattaacaa gagcgtgact
    16741 atagcggtac ggttagactc tttttcgtaa atagcaaagg tcttcttaaa attcatatcg
    16801 tttcctacct caaaaaatca tcagtgatct gatttttagt tgataaaata ttctattttt
    16861 aaaaagtagt gacctaaatg atagccagtg gtctagagaa tcttgacagt tttttaaggc
    16921 gagtgaaact tagactcaaa tgctgatttg ggagcggttt tctgatataa tagagttatt
    16981 atagtagggc aaaggagcac agttgcaagg taaaatcatt aaatctctgg ctggttttta
    17041 ttacgtggag tcagaaggac aggtatatca gacgcgagcg cgtggtaatt ttaggaagcg
    17101 cggtgagaca ccttatgttg gagatatcgt ggatttctca gcagaggaca actctgaagg
    17161 ttatatttta gcgattcatc ctcgtaaaaa cagtttggta agacctccta tcgtcaatat
    17221 tgaccaagca gtggttatta tgtctgctaa agaaccagaa tttaacagca atcttctaga
    17281 ccgctttctc atcttgcttg aacacaaagc cattcatcca gtggtttata tctctaagat
    17341 ggacttatta gatagtcccg aggagattaa ggctattggt cgacagtatc aggcaatcgg
    17401 ttacgatttt gtgaccagtc ttgaggaatt gttgcctttg ctagctgata aaattaccgt
    17461 ctttatggga caaacaggtg ttgggaaatc aacccttctc aaccgaatcg cccccgaatt
    17521 ggctcttgaa acaggagaaa tctcagatag tctagggcgt ggtcgtcaca ccacccgagc
    17581 tgtgagtttt tacaataccc atggcggaaa aatcgcggat acgccaggtt tctcatcatt
    17641 agactatgat attgctaatg ctgaagattt gaacgaggct tttccagagc tgcgccggct
    17701 gagccatgag tgtaagttta gatcctgtac ccatacccac gaacccaagt gtgctgtcaa
    17761 ggcagcgctt gaaacgggag agttgtggcc agttcgttat gagcattacc tgcaatttct
    17821 cagtgaaatt gaaaaccgtc gtgaaacata taaaaaagtt atcaaaagaa agtaggtcta
    17881 ctccatgtca actctaaaaa ttgccccatc tattctagca gctgattacg ctaattttgc
    17941 ttctgagcta gctcgtattg aagaaacaga tgccgaatac gttcacattg acattatgga
    18001 tggtcaattt gttcctaaca tcagttttgg tgcagatgtt gtggcaagta tgagaaagca
    18061 cagtaaattg gtttttgatt gtcatttaat ggtggttgat cctgaacgct atgttgaggc
    18121 ttttgcacaa gctggtgctg atatcatgac cattcacaca gaaagtactc gtcatattca
    18181 tggagccctt caaaaaatca aggcagctgg catgaaagca ggggtagtga ttaatccagg
    18241 aactcctgca acagccctag aacctttgct agacttagtt gaccaagtcc tcattatgac
    18301 agtcaatcca ggttttgggg gtcaggcctt tatcccagaa tgtttagaaa aagtggctac
    18361 cgtagccaaa tggcgagatg aaaaaggatt gtcctttgac attgaggttg atggcggagt
    18421 agacaataag accattcgtg cttgttatga agcaggggcc aatgtctttg tggcaggttc
    18481 ttaccttttc aaggccagtg acctagtgag ccaagtccaa accttaagga cggccttgaa
    18541 tgtctaaagt tgctcttttt gcgggaggag atctctctta tatcagccgt gattttgatt
    18601 attttgtcgg tattgataga gggagcctat ttttattaga aaatgggctt cctttgaata
    18661 tggctgttgg tgattttgat tcggttcccc aaaaagcgtt tacggatata aaagagaagg
    18721 cagagctttt tattactgct catccagaaa aaaatgatac ggatacggaa ttagccctta
    18781 aagaggtctt tgctcgtttt cctgaggcgg aagtgaccat ttttggggct tttgggggtc
    18841 gaatggacca tctcttatcc aatatttttt tgcctagcga ccctggtatt gctcccttta
    18901 tggcacaaat cgccttgcgt gaccaacaaa atatgctgac ctaccgcccg gcaggtcagc
    18961 accttatcca tcaagaagaa gggatgacct atgtggcatt tatggcagaa ggagaagctg
    19021 acttgaccat tactggtgct aaatttgagt tgacgcaaga taattttttc aaaaagaaaa
    19081 tctattccag taatgccttt atccatcaac ctattacagt tagtttgcca agtggctact
    19141 tgattattat tcaaagtaaa gattggagtt agtatggacc ttatcttgtt ccttttggtc
    19201 ttggttctct taggtttagg ggcttatctg ttgttcaaag tcaacggcct tcaacatcag
    19261 cttgcccaaa ccctagaagg caacgcggat aatttgtctg accaaatgac ctaccagttg
    19321 gatacagcta acaagcaaca attgttagag ctaacacagc tgatgaaccg acaacaagca
    19381 gacctttacc aacaattaac agatattcgt gacgtcttgc accgtagttt gtctgatagt
    19441 agagaccggt ctgacaaacg cttagaagaa attaaccagc aggtcaacca atcgctcaaa
    19501 aatatgcaag aatctaacga aaaacgtttg gaggaaatgc gccagatcgt tgaagaaaaa
    19561 ttggaggaaa ccttaaaaaa tcgtctgaac gcctctttcg attctgtatc caagcaacta
    19621 gaaagtgtca ataaaggctt gggagaaatg cgtagcgtgg ctcaagatgt gggtacttta
    19681 aataaggttt tgtccaatac caaaacacga ggcattttag gcgaacttca actaggccaa
    19741 atcattgagg atatcatgac atcaagccag tacgaaagag aatttgtaac ggttagtggt
    19801 tctagtgaac gcgtagaata tgcgattaag ctcccaggaa atggtcaagg cggctatatt
    19861 tacctaccga ttgactcaaa attccctctt gaagattatt accgattaga agatgcttac
    19921 gaagttggtg ataaactggc catcgaggct agccgaaaag cacttctggc agctatcaaa
    19981 cgctttgcca aagacattca taaaaagtac ttgaaccccc cagagacgac caatttcgga
    20041 gttatgttct taccaacaga aggtctttat tcagaagtgg tcagaaatgc ggctttcttt
    20101 gatagccttc gtcgggaaga aaatattgtg gttgcaggtc cttcgaccct gtctgctttg
    20161 ctcaattcct tatctgttgg tttcaagacc cttaatatcc aaaaaaatgc tgatgacatc
    20221 agtaaaattt tgggcaatgt caagttagaa ttcgataaat ttggcggcct gcttgccaag
    20281 gctcaaaagc aaatgaatac agctaataat acgctggatc agctcatttc aacaaggaca
    20341 aatgccattg ttcgagcctt gaataccgtt gaaacttatc aagaccaagc aacaaaatct
    20401 ctcttgaaca tgcccttatt agaagaggaa aataatgaaa attaatcaaa tgaaaaaaga
    20461 ccagcttttt gaagggttct acctcattaa gtcggcagag gttcgtaaga caagggctgg
    20521 taaagatttt attagcctta cctttcagga tgacactggc gagatttcag gaaatctgtg
    20581 ggatgcccaa ccttataacg ttgaagaatt taccgctggt aaagtcgtgt ttatgaaagg
    20641 gcgacgcgag gtctataatg gtacccctca ggttaatcaa atcaccttgc gaaatgttcg
    20701 cccaggggag cccaatgatc caaaggactt caaagaaaag gcacctgtca gtgttgaaga
    20761 agtacgtgac tatttggaac agatgctctt taaaattgaa aatgcaacgt ggcaacgaat
    20821 tgtaagggct ctttatcgta agtatgataa agaattttat acttatcctg cagctaaaac
    20881 aaaccaccat gcttttgagt caggcctcgc ctaccatact gctacgatgg tcagactagc
    20941 agatagcatt ggagatatct accctgactt aaataagagt ctactgtttg caggtattat
    21001 gttacatgac ttagctaaag tcatagaatt aacaggacca gataatacag aatacaccgt
    21061 tcgtggtaac ttgattgggc atatttctct cattaacgaa gaaattacaa aagtaatttc
    21121 agaactacaa atcgatgata ccaaagaaga agtcattgtc cttaggcacg tgattttaag
    21181 ccatcacggg caattagaat acggtagtcc ggtacgtcca cgcatcatgg aagctgaaat
    21241 tattcatatg attgacaata ttgatgctaa tatgatgatg atgacaacag ctcttagccg
    21301 tgttagcgag ggcgagatga ccaatcgtat ttttgccatg gataatcggt ctttttacaa
    21361 gccaaactat tgatcagtgt ttctcaagta atagttactt tgcttttgtg atgatttttg
    21421 atgtgcttaa cacttatgat ggtttcataa gtgtttttgg ctcttgttgt ggttattaag
    21481 tagaatgtcc tttcagtagg aatgttgatt agtttatctt agattgaatt cttg
//
LOCUS       DAUGFP010000027        20939 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.027, whole genome shotgun sequence.
ACCESSION   DAUGFP010000027 DAUGFP010000000
VERSION     DAUGFP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20939
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.027"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(397..783)
                     /gene="rplQ"
                     /locus_tag="VH663_001049"
     CDS             complement(397..783)
                     /gene="rplQ"
                     /locus_tag="VH663_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEQ8174258.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(798..1736)
                     /locus_tag="VH663_001050"
     CDS             complement(798..1736)
                     /locus_tag="VH663_001050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEQ8174259.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(1782..2165)
                     /gene="rpsK"
                     /locus_tag="VH663_001051"
     CDS             complement(1782..2165)
                     /gene="rpsK"
                     /locus_tag="VH663_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEQ8174260.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2183..2548)
                     /gene="rpsM"
                     /locus_tag="VH663_001052"
     CDS             complement(2183..2548)
                     /gene="rpsM"
                     /locus_tag="VH663_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEQ8174261.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2566..2682)
                     /gene="rpmJ"
                     /locus_tag="VH663_001053"
     CDS             complement(2566..2682)
                     /gene="rpmJ"
                     /locus_tag="VH663_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEQ8174262.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2708..2926)
                     /gene="infA"
                     /locus_tag="VH663_001054"
     CDS             complement(2708..2926)
                     /gene="infA"
                     /locus_tag="VH663_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEQ8174263.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3044..3682)
                     /locus_tag="VH663_001055"
     CDS             complement(3044..3682)
                     /locus_tag="VH663_001055"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEQ8174264.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEELGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(3832..5136)
                     /gene="secY"
                     /locus_tag="VH663_001056"
     CDS             complement(3832..5136)
                     /gene="secY"
                     /locus_tag="VH663_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEQ8174265.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(5153..5593)
                     /gene="rplO"
                     /locus_tag="VH663_001057"
     CDS             complement(5153..5593)
                     /gene="rplO"
                     /locus_tag="VH663_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEQ8174266.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(5808..5990)
                     /gene="rpmD"
                     /locus_tag="VH663_001058"
     CDS             complement(5808..5990)
                     /gene="rpmD"
                     /locus_tag="VH663_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEQ8174267.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(6005..6499)
                     /gene="rpsE"
                     /locus_tag="VH663_001059"
     CDS             complement(6005..6499)
                     /gene="rpsE"
                     /locus_tag="VH663_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEQ8174268.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6518..6874)
                     /gene="rplR"
                     /locus_tag="VH663_001060"
     CDS             complement(6518..6874)
                     /gene="rplR"
                     /locus_tag="VH663_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEQ8174269.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(6979..7515)
                     /gene="rplF"
                     /locus_tag="VH663_001061"
     CDS             complement(6979..7515)
                     /gene="rplF"
                     /locus_tag="VH663_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEQ8174270.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(7718..8116)
                     /gene="rpsH"
                     /locus_tag="VH663_001062"
     CDS             complement(7718..8116)
                     /gene="rpsH"
                     /locus_tag="VH663_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEQ8174271.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(8267..8452)
                     /locus_tag="VH663_001063"
     CDS             complement(8267..8452)
                     /locus_tag="VH663_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEQ8174272.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(8468..9010)
                     /gene="rplE"
                     /locus_tag="VH663_001064"
     CDS             complement(8468..9010)
                     /gene="rplE"
                     /locus_tag="VH663_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEQ8174273.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(9034..9339)
                     /gene="rplX"
                     /locus_tag="VH663_001065"
     CDS             complement(9034..9339)
                     /gene="rplX"
                     /locus_tag="VH663_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEQ8174274.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(9418..9786)
                     /gene="rplN"
                     /locus_tag="VH663_001066"
     CDS             complement(9418..9786)
                     /gene="rplN"
                     /locus_tag="VH663_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEQ8174275.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9811..10071)
                     /gene="rpsQ"
                     /locus_tag="VH663_001067"
     CDS             complement(9811..10071)
                     /gene="rpsQ"
                     /locus_tag="VH663_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEQ8174276.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(10097..10303)
                     /locus_tag="VH663_001068"
     CDS             complement(10097..10303)
                     /locus_tag="VH663_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEQ8174277.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10313..10726)
                     /gene="rplP"
                     /locus_tag="VH663_001069"
     CDS             complement(10313..10726)
                     /gene="rplP"
                     /locus_tag="VH663_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEQ8174278.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10730..11383)
                     /gene="rpsC"
                     /locus_tag="VH663_001070"
     CDS             complement(10730..11383)
                     /gene="rpsC"
                     /locus_tag="VH663_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEQ8174279.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11396..11740)
                     /gene="rplV"
                     /locus_tag="VH663_001071"
     CDS             complement(11396..11740)
                     /gene="rplV"
                     /locus_tag="VH663_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEQ8174280.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11756..12034)
                     /gene="rpsS"
                     /locus_tag="VH663_001072"
     CDS             complement(11756..12034)
                     /gene="rpsS"
                     /locus_tag="VH663_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEQ8174281.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(12173..13006)
                     /gene="rplB"
                     /locus_tag="VH663_001073"
     CDS             complement(12173..13006)
                     /gene="rplB"
                     /locus_tag="VH663_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEQ8174282.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(13024..13320)
                     /locus_tag="VH663_001074"
     CDS             complement(13024..13320)
                     /locus_tag="VH663_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEQ8174283.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13320..13943)
                     /gene="rplD"
                     /locus_tag="VH663_001075"
     CDS             complement(13320..13943)
                     /gene="rplD"
                     /locus_tag="VH663_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEQ8174284.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(13967..14593)
                     /gene="rplC"
                     /locus_tag="VH663_001076"
     CDS             complement(13967..14593)
                     /gene="rplC"
                     /locus_tag="VH663_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEQ8174285.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14841..15149)
                     /gene="rpsJ"
                     /locus_tag="VH663_001077"
     CDS             complement(14841..15149)
                     /gene="rpsJ"
                     /locus_tag="VH663_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEQ8174286.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(15348..16637)
                     /locus_tag="VH663_001078"
     CDS             complement(15348..16637)
                     /locus_tag="VH663_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEQ8174287.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKLPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITFH"
     gene            complement(17025..18041)
                     /gene="adhP"
                     /locus_tag="VH663_001079"
     CDS             complement(17025..18041)
                     /gene="adhP"
                     /locus_tag="VH663_001079"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEQ8174288.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNIGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADILVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVAPVVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(18293..20935)
                     /gene="adhE"
                     /locus_tag="VH663_001080"
                     /gene_synonym="adhC"
     CDS             complement(18293..20935)
                     /gene="adhE"
                     /locus_tag="VH663_001080"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEQ8174289.1"
                     /translation="MTEGHNTVETTSVSVTIDALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSTHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIVDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKTADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
BASE COUNT         6003 a         4467 c         3792 g         6677 t
ORIGIN      
        1 gtttgagcat ctgtttgcga tgcaagtatt ttttcgaaca tttaaatgag aggattacac
       61 cgtttatttg cccatgagag gtctttaaac acctgcataa ggttttctta ttctaatgac
      121 cttattgtct ctgagaaaac tttataaacg catcagaaga ggcttctgtg ttattgtttt
      181 actcagctta aaagcgggag tgtcacctca taatacaaca aaacgcctgc ttataaaaag
      241 tttctatagc ttagatacct ttcataaaca agcgttacat attaacaata tgatgagtgt
      301 tcccgctagg aaaaatccta gcggtagacc agagctagac taagaataat ctctcgatta
      361 ctccatcatc ataacactca acaaaattga taaaaattaa actaattcaa taattgccat
      421 tggagcagca tctccacggc gtggttctgt tttaagaata cgagtgtatc caccgttacg
      481 ttctgcataa cgaggtgcaa tttctgagaa aagtttttga agagctgtcg ttgatgtgta
      541 tttatcggta gcttcatcat aattttctga tgcgatttcg ttacgaacgt atgcagctgc
      601 ttggcgacga gcatgaagat caccacgttt accaagagta atcatttttt caactgtttt
      661 acggatttct tttgcacgtg cttctgtcgt tacaattgat tcgttgatca aaagatctgt
      721 cgtcaaatca cgaagcattg ctttacgttg tgagctagtg cgtcctagtt tacggtaagc
      781 cattttgtcc tcctatatta tttatcgttt tttagtccga gacctaagtc agcaagttta
      841 accttaactt cttcaaggct cttacgtccc agattacgga ctttcatcat ttcaggctca
      901 gatttttctg ttaagtcgaa gacagtgtta atgcctgcac gttttaaaca gttatatgag
      961 cgtacggata aatcaagctc ctcgatggtg cggtcaagta ccttttcatc gttcactttt
     1021 tcagtttctt tcattacttc ggtagctttt gcaacctctg ttaaatcagt aaacaggttt
     1081 aagtgttcga tcaaaactcg agcagataga cctaaagcat cttcaggaat aattgttccg
     1141 tttgtcatga tttcaatcgt taatttatca aagccatcat tgctacctac acgggcaggt
     1201 tcaacttgat aattaacttt tttaactggc gtatagattg aatctactgc caatgtaccc
     1261 acaggtgcat catctttttt gttgccttct gctggcacat aaccacgttt tttagcaaca
     1321 gtcattgttg cacgtagaga atgtccttca gcgattgtaa aaagataatg atccgggtta
     1381 acaagttcga tatcactgtc agtaagaata tcaccagctg ttacctctgc tggtccttct
     1441 acttcaagtt cgataatctt ttcgtcttcg acgtatgatt tcacagcaag tcccttaaca
     1501 ttaaggataa tttgcatgac atcttcacgt acacctggga ttgtatcaaa ttcgtgtagt
     1561 actccatcaa ttttaattga tgttactgct gcacctggaa gtgaagacaa gagtacacga
     1621 cgaagagaat tacctagagt tgttccgtag ccacgttcaa gtggttcgat gacaaatcta
     1681 ccgtaatctt tattttcatc aatttttgtt attattggtt tttcaaactc aatcattttt
     1741 tcacccctcg aaacgaaact tgtgtactat ttgttatatg attatacacg acgacgtttt
     1801 ggaggacgag caccattatg tggcacaggg gtcacatcac gaattgcagt tacttcaaga
     1861 cctgcagctg ctagagcacg aatagctgat tcacgacctg aaccaggacc ttttacagtc
     1921 acttcaacag tttttagtcc gtgttcttgt gcagattttg cagctgcttc tgcagccatt
     1981 tgagcagcaa atggagtaga tttacgagaa cctttgaaac caagagcacc agctgatgac
     2041 catgcaagag cattaccatg cacatctgta atcataacaa tagtgttatt aaatgtagcg
     2101 tgaatgtgtg caacaccaga ttcgatgttc tttttcacac gacgtttacg tgttggttta
     2161 gccaattgtt ttacctccta ttttattttt tcttacctgc aatcgcaaca gctttccctt
     2221 tacgagtgcg agcattgttt ttagtatttt gtccacggac aggaagtcca cgacgatgac
     2281 ggattccacg gtatgaaccg atttccatca aacgtttaat gttcatgttt acttcacgtc
     2341 gaaggtcacc ttcaactttg attgcatcca cttcacgacg gatagcatct tcttgatctg
     2401 atgttaaatc tttaacacgg atatcttcag aaatacctgc agctgctaag atttttttag
     2461 atgttgcaag accaattccg taaacataag taagtgaaat tactacgcgt ttatcatttg
     2521 gaatatcaac tccagcaata cgagccattt tttctccttt ctattttatc cttgacgttg
     2581 tttgtgtttt ggatttgtcg gacaaattac cataacacga ccattacggc gaataacttt
     2641 acagtattcg caaattggtt taaccgatgg tcttaccttc atgttttaat ccctccaatt
     2701 atttcgatta tttaaagcgg tatgtgatgc gtccacgtgt caagtcataa ggactcattt
     2761 ctactgtaac acggtcacct actaaaatac gaatgtaatt tttgcggatt tttcctgata
     2821 cagttgctag aatttgatgt ccattttcca attcaacagt aaacattgca tttggcatcg
     2881 tttctactac tttgccttca atttcaatca cgtcttcttt tgccacgcaa aagtaccccc
     2941 taaatttcga tttgatgtcc tctgagcaca gaggtaacaa ttataagtca gactaaacta
     3001 gtatatcatt actaatctac ttatgcaagt agattagtat aatttatttg agttctagca
     3061 acgctttttc aacatctgca aaaacgtctg ttatttcttg attaccttca atatctgtaa
     3121 caagaccaag cttacgatag tgttcaagaa taggttctcc ttgagccata ttgacgtcca
     3181 aacgacgttt aacagtttca ggcttatcat cttcacgttg atagtaatct tcttctttat
     3241 aatctactgg tgggttgaac actttatgga acgtttcacc agttttacga ttgataatac
     3301 gaccactcaa acgctctaca agacatgatg gatccacttt aatattaata acaccatcta
     3361 agcggagtcc tagttcttca agtgttgcat ctaaggcgtg tgcttgttca atagtacgtg
     3421 gatacccatc aagcaaaaaa cctttttctg cgatatcatc ctcagctaag cgctctttta
     3481 caatcccgtt tgtgacttca tcaggaacca attcaccttt atcaatataa cttttagcta
     3541 aacgtcccat ttcggtttga ttagccattg cggcgcggaa catatcccct gttgagatgt
     3601 gagcaacacc aagttcttca acaatcttag ctgcttgggt ccctttacca gctcccggca
     3661 aacccatgat taaaagattc atcttttcct cctcttttaa aataaataca tagtaggaaa
     3721 ttattttcct tggtttttgt tttcaaatgg caacaagaaa tcgtttgatg ataaccaagt
     3781 cttattgtca tttaaaaaca aaatagaagg ttgacaatgt caacctgtat tctattctgc
     3841 tgtattcata aatccgacat attttctctt tagaagatac ccctcaagct gtttcatacc
     3901 ttcgatacca gttgaaatca aaatgagcaa actagttcca cctaaagcaa tactagaaga
     3961 aaggttgaga gcttgttgtg ctgctattgg cgctaaagag ataaatgcta agaagatagc
     4021 tcctacggtt gctagttttt ttagcaatgc ggacataaat tgttctgtct cacgtccagg
     4081 gcgaacactt ggtatatatg aggaattctt ctgaagattt tctgctgtct tctcaggatt
     4141 aacttgtaca aaggtataga agaatgagaa caatataatc aacaaggcgt aaactatcat
     4201 tccgactgga gtttgataat taaaaatttc ttgcaaacgg tttaaccacg gtaaatctct
     4261 accattttga acaaaaggaa taatcgtact tgggatagtt gtaatcgagc tagcaaagat
     4321 aacgggaata acgccggctg gatttacttt taatggaaga tatgaacttg taggtgcacc
     4381 ttgcattagc tttgtgtatt ggattggaat tttatattcc gcttgttgga catatgttgt
     4441 aaagaaaaca atagcaagaa cagctatgat taaaatccca acaatgagat aagacgagtg
     4501 taaatcactt gccttaacat ttacaaaata gtcttcacga attgttgcaa ttgcacttgg
     4561 aatagacgaa ataatacccg caaagataat cattgatacg ccattaccaa atcctttatc
     4621 tgtgatttgt tctccaagcc aggtaacaat aacgctacct gttgtcagta atgccccgat
     4681 cagcaaatat gttttgatgt ctggtgtctt aacaagtgca acgttcgaca aagtattaaa
     4741 tcctgcagtg ataccaatgg attgggcaaa agccaaaaca agtgaaatat agcgcgtcgc
     4801 ttgatttaat ttacgacgtc caacctcacc ttgtttgccc cactcaacaa acttaggtaa
     4861 aatatccatc tgcaataact gaacaacgat agaagcagta atatatgggc taacccccat
     4921 tgaaaatact gagaaatttc tcatcgcatt accactaact aagtttaaca tatttaagaa
     4981 agggagttca cttaattgct ctaagctctt ggcatttaca ccaggtacgg taatatgtgt
     5041 cccaattcgg aatacaagaa tgataaatat agtaaagaaa attttattcc gtacagtctt
     5101 tatctttaac gcgtctttta gtatttttaa gaacataatg agtatcccct cattagatga
     5161 cttcgattga accaccttta gcaataatag ctgcttcagc agattttgag aacttagctg
     5221 cttttacagt caatttttta gtcaattcac cgttaccaag cactttaaca cctgattttt
     5281 cagctcggac aataccagca tcttttaaaa tagccggagt cacttctgta ccatcatcaa
     5341 aaacattcag ttgatcaaga ttcacaagtg catattcttt agtattaata tttgtaaagc
     5401 cacgttttgg aatacgacgg aacaatggtg tttgtccacc ttcaaaacct aaacgtacac
     5461 cgccaccgct acgagctttt tgaccttttt gaccgcgacc agatgtttta ccattacctg
     5521 atgatgtccc acgaccaaca cggttacgta ctttacgtga gccttcagca gcttttaatt
     5581 catgaagttt cattttttct cctttttttg taaaatgcta gcgcctctat gagggaaaag
     5641 gactccctat agaaactcgc ctatacatta atgatactta agttatccaa aataacttaa
     5701 tacagatacg cactaaccga ctactgtttg ttgccaaaaa agcaaactac aagagtcggt
     5761 tttgtgctcg tttttaatta tgaaatcaga gatttcacag ttataactta tttaacatct
     5821 tcaacggtaa ccaagtgaga aatagctgtc accataccac ggatagcagc attatcttct
     5881 ttgactactg aagagtttaa tttaccaagt ccaagagcaa caacagtttt acgttgttct
     5941 ggcttacgtc cgataggaga cttagtcaaa gtaattttaa tttgagccat gatgatcccc
     6001 tttcttatgc taagtcagaa actgagatgc cacgaagtgc ggcaacttct tctgcacgtt
     6061 taagttgttt caatccttca acagttgcac gaacaatgtt gattggagtg tttgaaccaa
     6121 gagattttga agtaatatca gcaacacctg ctaattcgat gacggcacga actgcaccac
     6181 cagcagcaac tccagaacct tctacagctg gtttcaataa tactttagct ccaccgaagt
     6241 tagtgtaaac ttcgtgaggg attgttgtac caaccattgg tacttcgatc atgtttttct
     6301 tagcagcttc aactgcttta cggatagctt ctggaacttc ttgagcttta ccagtaccaa
     6361 atccaacacg accatttcca tcaccaacaa ctacaagagc tgcaaagcga agacgacgtc
     6421 cacctttaac aacttttgta acgcggttaa tcgcaacaac gcgttcttca agttcaactg
     6481 cattatcttt aaatgccatt attttgtgtc ctccctatta gaatttcaat ccgttttcac
     6541 gagctgcatc agccaaggct ttaacacgtc cgtgatagag atatccaccg cggtcaaaca
     6601 ccacttcaga aataccttta gccactgcac gttcagcaac aagtttgccg actacaacgg
     6661 cttgttctgt ttttgttcct ttagaaacgt ctttatcaag agttgatgca cttgcgagcg
     6721 ttacacccgc tacgtcatca ataacttgag cgtagatgcc tgtattagaa cggaatacgt
     6781 tcaaacgtgg gcgatcagca gttccagaga gtttaccgcg gacgcgacgg tggcgttttt
     6841 ggcggatttt gtttttatct ggtttcgaaa tcacaatttt cacctcttaa ttttaatcaa
     6901 tttgtctcat tgacaagttg tcgattgaac ttatcgagca tgttaaggtg caatacttcc
     6961 catacccgac taagaacatt atttacctgt tttaccttct ttaaggcgta cgtattcacc
     7021 aacgtaacgg atccctttgc ctttgtaagg ctctggtgaa cgcaagctac ggatgtaagc
     7081 agctgtttga ccaacaactt ctttgttgat tccttcaact gagattgaag ttgggttagc
     7141 aacagtgaaa gtaattcctt ctggagcttc aacttcgtct tggtgagatt tacctactga
     7201 aaggacaagt ttagtacctt gaagttgagc acggtaaccg acacccttca tttcaagatc
     7261 ttttttgaaa ccttcagaaa cacctacaac catgttattc aagttagcac gggttgtacc
     7321 atggattgtt ttcatttctt ttgagtcgtt aggacgtaca actgtgattt cagtcccttc
     7381 aactttgatt tcaatatttt tgttaaactc acgagtgagt tcgcctttag ggcctttaac
     7441 agtaataaca ttgttgttat ttgttaattc aacgcctgca ggcatagtaa ttactttatt
     7501 accaatacgt gacatgttta ttttctcctg ttaaattgtc aagccgcata gcgactagtt
     7561 ttcacggggg tttgatagaa tgatatactt caatttatat ttcaaaattg aacacgagct
     7621 gtggtctgtg tgaaaaagat aaatcttcct tgagtataaa cacgctacgt cagatttcct
     7681 attttcactt tgtccacgac gctcttagta tcttaaatta ccaaacgtaa gcgatcactt
     7741 ctccaccaac attcttttgg cgagcttctt tgtcagtcaa aagaccttct gatgttgaaa
     7801 tgattgcaat tccaagtccg ttaagaactt taggcatgtc atcacgtttt gcgtaaacgc
     7861 gaagacctgg ttttgaaata cgttttaagt tagtgataac acgctcacca ttttttccgt
     7921 attttaagaa cacacgaatg atgccttgtt tatcatcttc gataacttca acatttttta
     7981 caaaaccttc acgtttaaga atctcagcaa tccctttttt aatgtttgaa gcaggtactt
     8041 ctaacacttc gtgttttact tgattagcat tacggatacg tgttaaaaag tctgcaattg
     8101 ggtcagtcat aaccattttt tatattctcc tcttactagc agtttgatgc tatcacttac
     8161 tagttaatgt tgtccttaaa ggactttggt atcagcttat gctcacaccg ttttaattaa
     8221 gcacaagact taatctgaag aacaaattaa gttttgtgtc atatttttac caagaagctt
     8281 tgacaacacc tggaatttga cctttgtaag ccaattcacg gaagcaaaca cgacaaagtt
     8341 taaacttgcg gtaaactgaa tgtgggcgtc cacatttttc acagcgagta taagcttgtg
     8401 tagagtgttt tgcaggacgt ttgttcttag caatcataga ttttttagcc aatttattta
     8461 ccccctatta ttttgcaaaa ggcattccaa ggcccttaag caattcacgt gattcttcgt
     8521 cagtatttgc agtagtgacg attacaatat caagaccacg tactttatca acatcatcaa
     8581 agctgatttc tgggaagata agttgttctt tcacaccgag agtgtagttt ccgcgtccat
     8641 caaacgattt tgttggaacg ccatggaagt cacgtacacg aggaagtgaa acgctaacca
     8701 atttgtctag gaattcgtac atacgttcgc cacggagggt aacttttgca ccgatcgcta
     8761 caccttcacg aagacggaag ccagcgattg atttcttagc tttagtaata agtggttttt
     8821 gacctgagat aagtgctaat tcagctgcag ctttctctag gttttttgcg ttggatacag
     8881 catcaccaac acccatattg agaacaattt tctcaacttt tggcacagcc ataactgatg
     8941 tgtaattgaa tttctctgtc aacgcaggaa ttacttcgtt agtatatttt tcttttaaac
     9001 gatttgccat tatgcttctc ctttccttcg tgattaatca agcacttcgc ctgatttttt
     9061 gctgtaacgt acttttttac cgtcaacaac tttataacca acacgtccag ctacaccatt
     9121 tttatctaat acttgaacat tagagacatg gataggtgct tctttttcga cgatagctcc
     9181 ttgaggattt tcagtgttag gtttttggtg ttttttaatc attccaacac cttcaacaat
     9241 aactttgtta acttttggaa gtgctttaag aactacagct tcagtgcctt tgtccttgcc
     9301 agcaattacg cgaactttgt cgcctttttt tacaaacatt ctggtttctc ctattatttc
     9361 ttacgccccg atgggcaccc taggaaaacc taggggacta cgtttgttaa cctgagatta
     9421 aagtacttct ggtgcaagtg atacgatctt catgtagcca ccctcacgca attcgcgtgc
     9481 aactgggcca aagatacgag ttccgcgagg agttttatca tcacggatga ttacagcagc
     9541 attgtcgtca aatttgatgt atgaaccgtc tggacggcgt gcaccagttt ttgtacgaac
     9601 gataacagct ttaaccacat cacctttttt aactgctcca ccaggagtag cttgttttac
     9661 agaagcaacg attacgtcac cgatgttagc gaatttacgt cctgaaccac caagtacttt
     9721 gatagtcaag atctcacgag caccgctatt atcagcaact ttcaagcgag tttcttgttg
     9781 aatcatttca attttctcct tttagtttga ttagataata acagcttttt ccactacttc
     9841 cacaagacgg aaacgttttg tagctgaaag tgggcgagtt tccatgatac gaacgatatc
     9901 gccttcttta gcaacgttgt tttcgtcatg tgctttatat tttttagaat agttgatacg
     9961 tttaccatag actgggtggt tacgtttagt ttcaactaca actgtgattg ttttgtccat
    10021 cttgtcagac actacgcgtc cgtaaagagt tttacgttga ttacgttcca tttagaattt
    10081 ctcctttccc aatctattat ttcatttctg attgcacagt tttaacacgt gcaatttgtt
    10141 tcttaacttc gtcaaggcga gcagtctttt caagttgacc tgctgcagct tggaaacgaa
    10201 gatcaaacaa ttcttttttg agttcgtttt ctttcttagc aagttcttct tgagacaatc
    10261 cacgaagctc tttaacaaaa tctttaattt cttgaagttt catgtcttct ccttattctg
    10321 cttcacgttt tacgaatttg cacttaactg gtaatttgtg gctagcaaga cgtaatgctt
    10381 cgcgagcaat ttcttcagaa acaccagcaa tttcaaacat cactttacca cgtttaactg
    10441 gtgctaccca accttcaggt gcccctttac cagaacccat acgtacaccg atagcttttg
    10501 cagtgtatga tttgtgtggg aagattttaa tccaaacttt accaccacgt ttcatgtaac
    10561 gagtcatcgc aatacgggca gcttcgattt gacggtttgt aatccagtgg ctagttgtgg
    10621 cttgaagtcc gtattcaccg aatgaaactt ctttaccacc tttagcctca ccgcgcattt
    10681 ttccacggaa ttcacgacgg tgtttaacac gtttaggtac taacatttgt tatttgcctc
    10741 ctttagtgtt tttacgagct ggaagaactt ctccacggta aatccaaact ttaacgccaa
    10801 gtttaccata agttgtgtca gcttcttccc aagcatagtc gatatcagcg cgaagcgtgt
    10861 gaagtggaac agttccttct gaataacctt cagcacgagc gatatcagca ccgttcaaac
    10921 gaccagaaac ttgagtttta atccctttag ctcctgcacg cattgtacgt tggattgctt
    10981 gtttttgagc acgacggaaa gcaacacgtt gctcaagttg acgagcaatg ttttcaccaa
    11041 caaggtgagc atctaaatca ggttgtttga tttcgatgat gttgatgtgt acttgttttc
    11101 cagttaattt gttaagttga ccacgaagag cgtcaacgtt tgcgccacct ttaccgataa
    11161 ccatacctgg tttagcagtg tgaagtgaaa caataacttt gtttactgca cgttcaattt
    11221 caatagttga aactgatgcg tcagctaatt ctttattaat gaatttacgg attgcaagat
    11281 cttcatgaag gtaatccgcg tattcttttt cagcatacca tttcgcatcc cagtcacgga
    11341 tgattccgac acgcatacca attggatgta ctttttgacc cacgatttta cctccttatt
    11401 tttctgatac aactacagtt acgtgagttg tacgtttgtt gattggtgaa gctgaacctt
    11461 tcgcacgtgg acggaaacgt ttcattgttg gtccttcgtt agcgaatgtt tcagatacta
    11521 ccaagtttgc tttttccaaa ccaaagttgt tttctgcgtt agcaattgct gagttaagag
    11581 ttttctcaat aacacgagct gctttgtttg gagtgaattt taagattgcg attgcgtcag
    11641 caactttctt accacggata agatcaagta ctaaacgtgt tttacgaggt gaaacacgca
    11701 ctgtacgagc cattgcttta gctgaagtaa tttctgccat tgtgtcctcc tcctattaac
    11761 gacgtgtttt cttgtcgtca gctgcgtgac ctttgtaagt acgcgttggt gcaaattcac
    11821 caagtttgtg acctaccatg tcttcttgga tgtaaacagg tacatgttta cgtccgtcat
    11881 aaactgcgat tgtatatccg atgaaacttg ggaaaatcgt tgaacgacgt gaccaagttt
    11941 tgattacttt tttcttttcg tcgtttgctt gagcttcaac ttttttcatc aaatgctcat
    12001 cgacgaaagg tcctttttta agactacgtc ccattttgta gttttctcct ttaaataatg
    12061 taccacagcg gcttgaattc aagagcaaga ctccaaactc taccgagttg gcggaataaa
    12121 tagcaaagct attgtaacgc tatttcagca agttttaagc aacttactaa gattattttt
    12181 cgttacgacg acgaacgata agtttgtctg atttagcttt cttgttacga gttttaagac
    12241 caagcgctgg tttaccccat ggagttgatg gcgctttacg tccaactggt gctttacctt
    12301 caccaccacc gtgtgggtga tcgttagggt tcattacaga accacgaact gttgggcgga
    12361 tacctttcca acggctacgt cctgccttac caatgttaac aagtgattgt tgttcgttac
    12421 caacagtacc gatagttgca cggcatgtac caagaatcat acgaacttcg cctgattgaa
    12481 gacgaacaag aacgtattta ccttcttgac caagtacttg agcagaagct ccagctgcac
    12541 gaacaagttc tccaccttta ccaggtttca actcaatatt gtgaacaact gtaccgacag
    12601 gaatgtttgc taatggaagt gcgttaccaa ctttgatatc tgcatctgga ccagaaacaa
    12661 tacgttgacc tacttcaaga cctttaggtg caatgatgta agctttcaca ccgtcagtgt
    12721 aatgtacaag tgcgatgttt gcagtacggt ttggatcgta ttcgattgtt ttaacaactg
    12781 cttcaacgcc atctttgtta cgtttgaagt cgatcaaacg gtaatgacgt ttgtgtccac
    12841 caccttggtg acgaactgtg atgcgaccat tgttgttacg accagctttg ctcttaagag
    12901 aaacaagcaa tgatttctca ggcgtgcttg ttgtgatttc cgcgaaatcc aaagaagtca
    12961 tgttacgacg gccatttgtc gttggtttat aaactttaat acccacgtta tttcctcctt
    13021 agattattca gcttcagctg cgaacaactc gattgcttta gaatcagctg taagagtgat
    13081 gatagctttt ttagtttttg aagtgaaacc tgtgtaacga ccaacgcgtt ttgcttttgg
    13141 tttaacgtta acagtgttta cacttgcaac tttaactccg tcaaaagcag cttcaacagc
    13201 ttgtttgatc aaaagtttgt gtgcacgagt atcaacttcg aaagtgtatt tgcctgcttc
    13261 aagagcaatc attgacttct cagtgataac tggttttttg attacgtcgt acaaattcat
    13321 tatgcaagaa cctcctcaat tgtagagatt gcttctttag taacaagaag tttgtctgcg
    13381 ttcacgatat caagaacact tgcagttgct gcagttgcaa cggttacgtt tggaaggtta
    13441 cgtgcagaaa gtgctgcaaa ttcatttcct tcttcaacaa gaacaagtac ttttgtatca
    13501 atgctaagag ctgaaagcac ttttgcaaat tcagcagttt ttggtgctgc aaatgaaagg
    13561 ccttctacag ctacaaattt atcttcagca acttttgctg agtaaactga tttcaacgca
    13621 aggcgacgaa ctttttgtgg aagtttgtat ccgtatgaac ggggagttgg tccaaagaca
    13681 acaccaccac cacgccattg tggtgagcgg atagaacctt gacgagcgcg tccagttcct
    13741 ttttgacgcc atggtttacg tccaccacct gatactgctg aacggttttt aaccgcatga
    13801 gtaccttggc gaaggctagc gcgttggcta attacaacat caaaaacaac tgattcgttt
    13861 ggttcgatac cgaagatagc gtcgtttaat tcaactgagc taacttcttt accagtttgg
    13921 tcaaatagtt ttacgtttgc cattttaact gttttctcct ttcttattat ttagcagctt
    13981 taactgctga cttgatagtg ataagagatt tcttagcacc tggtacgtta cctttaataa
    14041 ggataacgtt cttttctggg ataacttgta caatttcaag gttttgaact gttacacggt
    14101 taccacccat acgtcctgcc aagcgtttgt ttttgaaaac gcggttaggc gcaacaggtc
    14161 ccatagaacc tgggcgacga tggtaacgag aaccgtgagc cataggacca cgtgattgac
    14221 catggcgttt gataacacct tggaaacctt taccttttga tgtccctgtg acgtcaacaa
    14281 catcaccagc ttcaaattgt tctacagata attctgcacc aacttctaag ccttcaatgt
    14341 ttttgaattc acgaatgaag cgcttaggag ctgtgtttgc ttttgcaaca tggcctttgg
    14401 caggtttgtt actcaagact tcacgtttgt catcaaaacc aacctgaact gcttcataac
    14461 cgtctgtttc aacagtttta acttgaagca caacgtttgg agttgcttca atgacagtaa
    14521 cagggatgaa ttcgcctgat tcagtgaaga tttgagtcat tcccactttt ttccctaaga
    14581 ttccttttgt catgagaaaa tatttccttt tcttaataat atttcaaaaa gttttttatg
    14641 agcgtttttt gtgctcaaaa aagtttttta cgagcgtttt ttgtgctcat agcaagatac
    14701 aaagagcgtc aaactcaaaa taaaaataga aaatctaaca caaaaacttc tgtttcgagg
    14761 aagatttatc tttttcatgt agagtttagc tcgtgttcaa gttccatcaa acactaactg
    14821 tacttgcaaa atctcaccga ttaaagtttg atttctacgt tgacaccact tggaagatca
    14881 agtttcatca aagcatcaac agttttttgt gttggattga tgatgtctac caaacgtttg
    14941 tgtgtacgca tttcaaattg ttcgcgagaa tctttgtatt tgtgagtcgc acgaataatt
    15001 gtgtaaagac tgcgttcagt tggaagtgga actggtccag caactgtagc acctgtacgt
    15061 gttgcagttt caacgatttt ttctgccgct gtatcaagtg tacggtgttc gtacgctttc
    15121 aaacggatac ggattttttt gtttgccatc ttttctcctc tcgtctattt acgataatag
    15181 gctagctcct caagaaaacc gacacctgtt gcgtggcaat gcacccgagc gtgtcgcaac
    15241 ctcttgcatc attgctaaag ctgtattttt acagcaccac aatataataa cagaatcatt
    15301 tagtcattgc aagcttttta gggcattttt tcaaaaaaac taccttccta atgaaaggta
    15361 atttcttggt actttttaaa ttgtttagag agaataaacc accgactggt attatctaac
    15421 actgtcgcca tccagacccc tatgagtcca tattgccaaa ccacaccaat cacataacca
    15481 aggccaatgc gaatcaccca cattcctatg gttgttgcat agaaaggaag ctttgctttt
    15541 cccaaaccct gccagacggc tgtataaact aaagtgcctg ccgttgcggg agctcctagt
    15601 aaagaaaata gtaaaacaac catggcggac ctttgagcat cagtattctg agtaaatagc
    15661 ggcaagagac taggacctag cagataggtc aaggctccca taaccagcat catgagggtg
    15721 gataaaataa aagcttctct aataatatac cttatctcag tcaccttacc gccccccagt
    15781 tgcctggcca ctagaataat tgttgccgtt gccatggcta agccaggcat gtaattaaac
    15841 tgagtcaagg tttctccaat agcattacct gccaaggccg ttgtcccaaa gcgaacaaca
    15901 atgccaataa ttaggacatc tcctgctctc atcatcaaac gttcaccagc agcaggtagt
    15961 gataaatcaa aaataatttt gtccaaaggt ctcatcaatc gtttcgcaac ctgtttaata
    16021 ggaataaact gacaaagtaa aaacacaccc actaaacgag ataaaaccgt cgcccaagca
    16081 acaccaagaa gaccaaatcc ccatacatag atagacaagg cagaaaaaat ggcattgagt
    16141 acgtttatta ataagctcac ttgcatagga agcttaggat acccttgagc tcgaacaata
    16201 gctcctaagc tagttaaaag tcctagacta acaatcatac cgccgacaat ggatagatat
    16261 tgccctccaa ccaatgttac agaagcttca gctcctagcc attctaacac ctgatgatgt
    16321 ccaacaactg ataacaaccc taagccgacc gaaaggagga gtgttacttg caagacacct
    16381 gccatatagt ttaattgttt gttctgatta ttttctccaa tacttcgagc aatcagacta
    16441 gatacagcag ctcccagcgc gataaagagg gactgataaa tactgataat gttgttagct
    16501 atggagacac cagagactgc tacaagtcca atttgagcca ccaggtaatt atcaaccatt
    16561 cccattaaca tctgtaaaat attttctatc atagaaggca gagctaaaga gaaaattttt
    16621 cttctattat atatcattta tgtcccctaa taaaaaattc ttgatcaaac gattttttca
    16681 aacacagtca ctggactcat tcagttattt tggtccatta atgaattcat aaagtctatc
    16741 ttaatagcag agtcccaaga tacacgacac ttagtgctac agtaacaatt gaccacatgc
    16801 caatccttaa catttaccta acgtttataa ctaagaagat gaagaacaaa aggactccaa
    16861 acgaattttt tctattatat aatatattct tcatttagca actttcaatg tatcaaaata
    16921 gcactctcga gtcaaaaatg ttttctgata ctcgagagtg ctattttatt tggaatcaag
    16981 tttaagcaac ttaggcttat ctcatattta atacatttgt atgtttagct gatgaaatct
    17041 aagaccttgc gcccttggat taaaccacgt tccatttcat caaagacttc tggagcagtg
    17101 tcaactggaa ctttttcgac aaccggagca accagacctt ctgcaccaaa ggcaaaggct
    17161 tcttccaagt ctttacgtgt ccctacaagt gatccgacca ctttgatacc atctaaaaca
    17221 gtttttacga tagacagttc catgtattct gatggaagac ctacggctac aactgttcca
    17281 ccggcacgga cactatcaat tgcttggtta aaagcaactt tagaaacagc cgttaccaca
    17341 acaccatgtg ctccaccggt tttttcttga atatagcctg gaacatcttc aatttctttg
    17401 ccgttaacta aaatatctgc gccaacttct ttagctaact caagtttatc attattaatg
    17461 tccactgcga caacatgtgc attaaagact tttttagcgt attgaacagc cagatttcca
    17521 agaccacctg ccccaaagat aacaatccat tggcctggag cagcacctgc ctctttaata
    17581 gctttataag ttgttacacc agcacaagta atggaagaag cttgagccgg gtctaaacct
    17641 tctggaactt taactgcata atcagctgtt actactgcat attcactcat tcctccatca
    17701 acactgtatc cagcattttt aacactacga caaagtgtct cacgtcctgt agtacagtat
    17761 tcgcaatgac cacatccttc aaagaaccat gcaatagaga cacgatcacc aacctttaag
    17821 gatgttacgc cttctccaat ttcttctact ataccaatac cttcgtgccc caaaatacgt
    17881 cctggtacct gaccaaaatc gccatgtgca acgtgtaaat cagtatgaca aacgccacag
    17941 tattctactt taacaagtgc ttcaccatga ccaatatttg gaagatcatg ctcaacaact
    18001 tcgacacctg tactagcttg attaacaact acggctttca tatcagtcct ccatctaata
    18061 atgacttttt tgtaatcggt tacacataaa ttgtacatct tttcacaatg aaagtcaata
    18121 gaaacctgaa aagtttctga aacttaactg gttaatctta tagctgtttg cctatctgca
    18181 aaatacttaa ctacattttc tgacactgga tataaatgat atatttagaa aataaaatga
    18241 tttcttatga ctttaatcat aagaaatcat ttcctggtat caacattaaa aatcacttac
    18301 gtcgtcctgg acgttcagca taaccatagt aagcatcttc aataatttct tgcatgtgat
    18361 caaccattgg aagacgtggg ttagcaggtg aacattggtc ttcataagca aggtaagcca
    18421 attcacgaga atgttctttc cattcatttt cgtcgatacc ttgagctttg aagttcattt
    18481 gaataccaac tcggcaaccc agatcataaa cagctttcgc ataagattcc actgcttctt
    18541 ctggtgtaga agctggaagc cctaacaatt tcgcaatgtc ttggtatttt tcatccgcac
    18601 ggtagtaatt atacttaggc catgtcgccg ttttagctgg acgagttcca ttatagcgga
    18661 taacatacgg caaaagaatt gcatttgtac gtccgtgtac ggtatggaat tgtgcgccaa
    18721 ttttatgcgc cattgagtga gaaataccta agaatgcatt ggcgaaagcc atacctgcca
    18781 tagttgacgc attgtgcatc ttttcacgtg cttcaaaatc ggctgtttta actgattttt
    18841 caagattatc aaaaacaatt ttaatggctt gaagagctaa accatccgta aagtcattgg
    18901 ccatttgtga cacgtaagct tcggttgcgt gtgtcaaaac gtccatacca gtatcagcag
    18961 caataaagcc tggaacagtc aataccaaag ctgggtcaac aatggctaca gttggtgtta
    19021 atgagtaatc agcgattgga tatttacgat tatttgcttt gtcagagata acggcaaatg
    19081 gtgtcacctc tgaacctgtt ccagaagttg ttgggatagc aacaaatttt gttttcttac
    19141 caagttctgg gaatttgaac gcacgtttgc ggatatccat gaatttttga acgaggtcat
    19201 ggaaatcaac ttctggttgc tcgtagaaga gccacataac tttggctgca tccataggag
    19261 atcccccgcc aagggcaata atggtgtctg gtttgaaggt acgcattaat tcagtacctt
    19321 tcataacagt tgtaatgtct ggatctggtt ctacttcagc aaagatctga taaacaactt
    19381 tattgcgacg aagatcaagt tgttcaatga tacgatccaa gaaaccaagc tcaaccatag
    19441 cgtgatctgt aacaatcatg acacgttcaa cgtcacgaca tttttgcaaa tattgaattg
    19501 aatcacgttc gaagtatgtt tttgaaggaa ctttaaacca ttgcatatta tttctacgtc
    19561 ttcctacttt tttgatgttc aagagattta tagcactaac gttatcacca actgcgttac
    19621 gtccatacga accacaacca agagtcaatg atggcaagaa agcattgtaa acatccccga
    19681 taccaccaaa tgtagaaggt gaattccaga tgacgcggat agcacggatt ctagttccaa
    19741 attcttttgc caactctgca tctgctgtat gaatggcagc tgagtgaccg agtccattaa
    19801 attcaaccat ttgacgagct ttttcaacac cgtcttcacg tgattcagat ttcaaaacag
    19861 caatgactgg agataatttt tcacgtgtta aaggctcatt ttctgaaact tctttacatt
    19921 ctgcagcaag aatattggtt ccttctggaa cagtaaatcc agcttgttct gcaatccaag
    19981 tagctggttt cccaacaatg tttggattca atttagcgcc agcacagttt ttactgttag
    20041 cttttgcacc aaaacagaat tcttcaagaa gggctttttc ttttttattt acaaaataag
    20101 tatggtaaga tttgaactct gcaacaaaat cgtcatagat ttctttgtcc acgataacag
    20161 cttgttcaga cgcacagacc ataccgttat caaatgattt agacatcaca atatcatgag
    20221 cggcttggcg gatgttagca gatttttcaa cataagctgg aacgttacca gcacctaccc
    20281 caagggcagg tttcccacat gagtatgccg ctttaaccat tgcatttcca cctgtcgcaa
    20341 ggatagtggc aatgccgtca tgattcatca aagcatttgt tgcttcaagt gatggtgttt
    20401 caacccactg aacacaattt tcaggagcac cagcggcgat agcagcgtct ctaacaatcc
    20461 gagcggcatg cgttgatgat tcttgtgctg atgggtgaaa ggcaaaaata attggattac
    20521 gtgtttttaa cgagatcaaa gatttaaaga tagctgttga agttgggtta gttgttggtg
    20581 taatcccaca gataacaccc accggctcag caattaatgt caaacctgtc acatcatctt
    20641 cttcaataat tcctactgtt ttttgatggc gcatgttatt aaccacatgc tcacaagcaa
    20701 atagattttt tgttgcttta tcttcaaaga caccgcgacc tgtttcttca taagcatgtt
    20761 tagcaagttc accgtgagca tccaaagcag caacagaagc tttggctacg atataatcaa
    20821 cttgttcttg gtcaagctta cgcatctctt caagagcagc taaacctttt tgaacaagag
    20881 catcaattgt cacagaaaca gaggttgttt caacagtatt atgtccttca gtcatttga
//
LOCUS       DAUGFP010000028        20294 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.028, whole genome shotgun sequence.
ACCESSION   DAUGFP010000028 DAUGFP010000000
VERSION     DAUGFP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20294
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.028"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..768)
                     /locus_tag="VH663_001081"
     CDS             complement(<1..768)
                     /locus_tag="VH663_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ8174290.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRM"
     gene            1012..2259
                     /locus_tag="VH663_001082"
     CDS             1012..2259
                     /locus_tag="VH663_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ8174291.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            2533..3894
                     /locus_tag="VH663_001083"
     CDS             2533..3894
                     /locus_tag="VH663_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ8174292.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFTYIYWCNLKSARNLYLFKQKGQKIPSFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINTKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            3894..4730
                     /locus_tag="VH663_001084"
     CDS             3894..4730
                     /locus_tag="VH663_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ8174293.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            4888..5685
                     /locus_tag="VH663_001085"
     CDS             4888..5685
                     /locus_tag="VH663_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ8174294.1"
                     /translation="MTSKITIKDIAREAGVSVATVSYVLNQRNDQKISEETRNKVLHI
                     SNLLNYTPNQAARSLVTQQSNSVVLYYKYADSILARAEQLHTISFLSRLLHEQDYQLV
                     CLNEDSIGKFDWADLILTLDIENDTFYKIGKLNFKPLIAIDSFVNDPLFFQINTDYAN
                     LKDQADQFFKDEPYTLLTLPINNQERYEAISTLFPNLHVVDHMRELPSFEKKNLLLID
                     ESLDQFFVNSSQKLYCPRFSPEKGQVILEAIEWAKQRQLNKNHNLLV"
     gene            complement(5870..6001)
                     /locus_tag="VH663_001086"
                     /pseudo
     CDS             complement(5870..6001)
                     /locus_tag="VH663_001086"
                     /inference="COORDINATES: protein motif:HMM:NF037788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6607..7467)
                     /locus_tag="VH663_001087"
     CDS             complement(6607..7467)
                     /locus_tag="VH663_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ8174295.1"
                     /translation="MRKTYVSTSMKRKRFFTQLLTYLYLISLAIVILFPILVTVSSAF
                     RPGNTTAFSFHFDGPWTLSNFKTLFQDTLYLRWYWNTLIVAFFTMLIQVTVITLTGYA
                     YSRYNFFGRKKSLIFFLVVQMVPTMAALTAYFVMAWLFNALNQYWFLILIYVGGGIPM
                     NAWLMKGYFDTVPYDLDESAKLDGSGHFRTFYQIVLPLVRPMIAVQSLWAFMGPFGDF
                     MLAKFLLRAQENYTVAVGLQSFITNDARNPKVTLFAAGAILIAVPISVLFFFLQKNFV
                     SGLTSGGTKG"
     gene            complement(7467..8771)
                     /locus_tag="VH663_001088"
     CDS             complement(7467..8771)
                     /locus_tag="VH663_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ8174296.1"
                     /translation="MVDNQRQQKPNKAMWLSVIPGLGQLYNKQIVKGGVLLVVFFLEL
                     LEIVVLGIPALTGLYSLGNVPMQDHSLFMLIKGAMQLITLVLFGIFHLVAMRDAKLVA
                     HQMNEGKKVPVTAKETLEAIYEKGFPYLLIIPAYLAMAFAIIFPVLVTLLIAFTNYDF
                     RHIPPYRLLDWVGLKNFLNILELSTFKTAFTSVLSWTLIWTLAATSLQIVIGIATAVI
                     ANQSFIRGKRLFGVIFLLPWAVPAFISIMSFGNFFNDSIGAMNVQVLPFIEKFLPFVD
                     FGIVSWKTDPFWTKVAVIMVQGWLGFPYIYILVSGILQAIPADLYEAATVDGVTVLQK
                     FRHITMPMIFAVAAPTLIAQYTFNFNNFSIIYLFNNGGPGTVGGGAGTTDILISWIYK
                     LTTQTSPQFSMASAVTLIISVIVISVSLITFKKFKAFDMEER"
     gene            complement(8846..10981)
                     /locus_tag="VH663_001089"
     CDS             complement(8846..10981)
                     /locus_tag="VH663_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrin glucanotransferase"
                     /protein_id="HEQ8174297.1"
                     /translation="MRELHIKTYKLLTKSAVLLGLISFPLTVSAADNASVTNKADFST
                     DTIYQIVTDRFNDGNTSNNGKTDVFDKNDLKKYHGGDWQGIIAKIKDGYLTDMGISAI
                     WISSPVENIDSIDPSNGSAAYHGYWAKDFFKTNQHFGTEADFQQLVKVAHQHHIKVVI
                     DFAPNHTSTAEKEGTTFKEDGALYKNGKLVGKFSDDKDKIFNHESWTDFSTYENSIYH
                     SMYGLADLNNINPKVDQYMKEAIDKWLDLGVDGIRVDAVKHMSQGWQKNWLSHIYEKH
                     NVFVFGEWFSGHTDDDYDMTTFANNSGMGLLDFRFANAIRQLYTGFSTFTMRDFYKVL
                     ENRDQVTNEVTDQVTFIDNHDMERFATKVANNQTAVNQAYALLLTSRGVPNIYYGTEQ
                     YATGDKDPNNRGDMPSFNKESQAYKVISKLAPLRKQNQALAYGTTEQRWISDHVLVFE
                     RKFGNHVALVAINRDQTNGYTITNAKTALPQNSYKDKLEGLLGGQELIVGADGTISSF
                     ELGAGQVAVWTYEGEDKTPQLGDVDASVGIAGNKITISGQGFGNSKGQVTFGEISAEI
                     LSWSDTLITLKVPTVPANYYNISVTTADKQTSNSYQAFEVLTDKQIPVRLLINDFKTV
                     PGEQLYLMGDVFEMGANDAKNAVGPLFNNTQTIAKYPNWFFDTHLPINKEIAVKLVKK
                     DSIGNVLWTSPETYSIKTGHEAQTITIKK"
     gene            complement(11007..12710)
                     /locus_tag="VH663_001090"
     CDS             complement(11007..12710)
                     /locus_tag="VH663_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEQ8174298.1"
                     /translation="MNVAGMLHIPDSRYCFVISQKELVIRLRVAKEDRGITVNLVHGP
                     KYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTT
                     SYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKDDSYVNMGW
                     LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQF
                     GTKYDLQELIDLAHQMGIKIILDAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEH
                     PSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHD
                     FWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTS
                     KQLAEKLSNILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGI
                     PCLYYGTEVAMEGGYDPDSRRGFPWDDAERDKDFFSKVKAIIALRQQPALQEGAISID
                     YDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQDGYIVFS
                     N"
     gene            complement(12876..14135)
                     /locus_tag="VH663_001091"
     CDS             complement(12876..14135)
                     /locus_tag="VH663_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ8174299.1"
                     /translation="MSWNWKKTSVLGTLSLASVLPLTACVSGGGKGVKETDGKTIVVS
                     VDEGYVDYIKSIKGEFEKEHKVTVKVKKEGMMDTLDKLSTDGPTGASPDVFLAPFDRV
                     GGLGTEGQIAEVTLGNSKEFDDTVKKLVTIDGKTYGAPDVIETLVTYYNKDLVPQAPK
                     SFTELEVLQKDSKFAFASEPGKSVGFLAKWTDFYYGYGLIAGYGGYIFGDKGTKPSDL
                     GLGNDGTVEGLNYAKQWYGTWPQGMQDTKKAGDFITEQFISKKAGVIIDGPWAASSFK
                     DAGVNFGVMEIPTLTNGKKYQPFAGGKAWVISNYSKGKTTAQKFLDYVTNAENQKRFY
                     DKTQEIPANLTARNYASKEGNELTKAVISQFENAQPMPNIPEMAEVWEPGANMFFNVA
                     SGKEEASKAAKEAAKTIKEAIEQKYAE"
     gene            complement(14750..15733)
                     /locus_tag="VH663_001092"
     CDS             complement(14750..15733)
                     /locus_tag="VH663_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ8174300.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGMQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTAPQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDYEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEHT"
     gene            complement(15764..17014)
                     /gene="dltD"
                     /locus_tag="VH663_001093"
     CDS             complement(15764..17014)
                     /gene="dltD"
                     /locus_tag="VH663_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEQ8174301.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(17007..17246)
                     /gene="dltC"
                     /locus_tag="VH663_001094"
     CDS             complement(17007..17246)
                     /gene="dltC"
                     /locus_tag="VH663_001094"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEQ8174302.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(17264..18520)
                     /gene="dltB"
                     /locus_tag="VH663_001095"
     CDS             complement(17264..18520)
                     /gene="dltB"
                     /locus_tag="VH663_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEQ8174303.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKAFMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(18517..20055)
                     /gene="dltA"
                     /locus_tag="VH663_001096"
     CDS             complement(18517..20055)
                     /gene="dltA"
                     /locus_tag="VH663_001096"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEQ8174304.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGERLTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELSSGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(20067..20210)
                     /locus_tag="VH663_001097"
     CDS             complement(20067..20210)
                     /locus_tag="VH663_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEQ8174305.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
BASE COUNT         6496 a         4135 c         3405 g         6258 t
ORIGIN      
        1 catacgaata gccagaacat caccaacaac aaccaaggca gttgctttaa aggaagatat
       61 agtttccatc aatatttcta cgctaacagg atccttacga tcaaataaca gcattggctt
      121 cgtttttaaa gatcgcttaa aacacccctt caaatagcca agataacgct cagaagttac
      181 ttctgatagt aaatcatctg ttataaataa tatgttgtga tggccttttt gataaaggtg
      241 ttctactgct gttttagcca ttagctgatt atcattatca atataagtgg ttttattagt
      301 gtcaccttgt ggagcaccta caatgacaaa tggtaagtta tttgtcatca aatatttttt
      361 gacaggatca tcttgatcag aataaagaat aataaaacca tcaactcgtt tttgaagatg
      421 catcagtttc acttgctcta tcaggtcatc aagagatata cctgtagcaa tcgacacagt
      481 aaaatgagat tctttagctt cgttagtaat agtcgacaaa atttccataa agaaaggttc
      541 acttaatcta tcgctagttg taataggagg aaaaacaagt cctatattgt gtgtcaaacc
      601 actcgctaga atctgtgctg cgacatttgg aacatatccc aaatctgcca tcgcttttct
      661 aaccttctct tttgttttca tagaaataga tcgattatct tttaataccc tactcaccgt
      721 cgaagggtta acacctgcct tttgagcgac atcttttatt gttaccatat taacctcgtt
      781 actatattta ccaaagataa accttatcat gggtttgtca tgtatttcgt ttccattata
      841 caattatctt tcaaaaaatc aacttcaatc aagattccct tgtcatttta actcaaattc
      901 tctaaactat ttttttaaaa aaacgacctt ttttttaaaa aaggcttgac tttttctgca
      961 agcggttgca taatattaag tgtaagaaat atatcttagg aggaaatagc tatgaaatca
     1021 tggcaaaaag ttatcgtcgg cggagcaagt ttgacacttg caagtacctt gttagtggga
     1081 tgtggatcag gttcaaaaga taaaaaggaa gctggcgctg actcaaagac tattaaactt
     1141 tgggtaccga ctggctctaa aaaatcatat gcagatacca ttgcaaaatt tgaaaaagat
     1201 tctggttaca ccgttaaagt ggttgaatca gaagatccaa aagcacaaga aaaaatcaaa
     1261 aaagatgcct caactgctgc cgatgtcttt tcacttcctc atgatcaact tggtcaactt
     1321 gtagaatctg gtactattca agaagttcca gaaaagtaca ataaagaaat tgctgctact
     1381 tctactgatc aggcgctcgt cggtgcacag tacaaaggga aaacatatgc tttcccattt
     1441 ggtattgaat cacaagttct tttctacaat aaatcaaaat tagctgctga agacgttact
     1501 tcatatgata caattactac aaaagcaact tttggtggta cctttaaaca agctaacact
     1561 tacgctactg gtccattatt catgtcagtt ggtaacactt tatttgggga aaacggtgaa
     1621 gatgtaaaag gtactaactg gggcaatgaa aaaggcgctg ctgttcttaa atggatcgca
     1681 gaccaagctt caaataaagg ttttgttagc ttagatgcta acaacgttat gtctaaattt
     1741 ggtgacggtt cagtagcttc ttttgaatca ggaccgtggg actatgaagc tgctcaaaaa
     1801 gctatcggta aagaaaacct tggtgttgct atttatccaa aagtaactat tggaggcgaa
     1861 acggttcaac aaaaagcatt cttgggtgta aaactttacg cagtcaacca agcaccagct
     1921 aaaggtgata caaaacgtat cgcagctagc tacaaattgg catcctactt gactaatgct
     1981 gaaagccaag aaaaccaatt caagactcgt aacattgtcc cagccaacaa agaagttcaa
     2041 tcttcagaag ctgttcaatc aaacgaactt gctaaaaccg ttatcactat gggttcttct
     2101 tcagactaca ctgtcgtaat gccaaaactt agccaaatgg gcacattctg gactgaaagt
     2161 gctgctattc ttagtgatgc cttcaacggt aaaatcaaag aaagcgacta ccttactaag
     2221 ttgcaacaat tcgacaaaga tatcgctgca acaaaataat atgatctcat gtcatcactg
     2281 ctatactaaa acaagcacaa ggtaagtact tctaaaaagt tctttccact taccttatga
     2341 agagtggaac taggctccat cacattttat tcaagcttag tctcactctt acttgttttt
     2401 gttttactag tagcttagtg ctacttaaat ttttaactct ttaaaagttt acgtaacact
     2461 tagctatttc atcctaacaa gagattgaat ctgaactacc actctagtat cctaaaataa
     2521 aggagaatct atatgactca gtcaaacttg acaccaaata tatctgtaat agaagcctta
     2581 aaaagaggca gttgggatat taaactttcg agtattatca tgggatttgc caactttgct
     2641 aacaaacagt ttatcaaagg aatcttattt ttaataagtg aacttatctt tctagtagct
     2701 tttgtctccc aaatcatccc tgctatccgt ggccttgtca ctcttggtac tcaaactcag
     2761 ggaatgacta caaaaactat tgatgggatt aacatccaag ttgctgttga tggcgataac
     2821 tctatgctaa tgcttatttt tggtttagca tcactcattt tttgcctagt ctttacttac
     2881 atttactggt gtaatctcaa aagtgctcgt aacctttatc ttttcaaaca aaaaggtcag
     2941 aagataccaa gcttcaaaga agatcttgcc acacttacaa atggtcgctt tcatatgact
     3001 ttaatggcta tcccactgat tggtgtgctt ttattcacca ttcttccttt gatttacatg
     3061 atctgtttgg cctttaccaa ttttgatcat aatcacttgc ctccaaaatc tttatttgat
     3121 tgggtgggac ttgctaactt tggaaatgtt ttaagcggcc gtatggcagg aacctttttt
     3181 cctatcttct catggacttt gatttgggct gtttttgcca cagtaactaa tttcttcttt
     3241 ggtattattc ttgctttgtt aatcaatacc aaaggattaa agtggaaaaa aatgtggcga
     3301 accatctttg ttatcaccat tgcagttcca caattcatct cattattgat tatgcggaac
     3361 ttactcaatg acgaaggccc actaaatgct cttctcaata aaatcggctt gattaatggc
     3421 tcactgccat ttttatctga tcctctttgg gcaaaattct caatcatttt tgtcaacatg
     3481 tggatcggta ttccatttac catgttgatt gcgactggta ttatcatgaa ccttccaagt
     3541 gaacaaattg aggctgctga aattgatggc gctagcaaat tccaagtctt caagtctatc
     3601 acattccctc aaattctctt gatcatgaca ccaaacttga ttcaacaatt tatcggaaat
     3661 atcaataact ttaacgtcat ttacctcctt actggtggtg gtccaacaaa ttcagaatac
     3721 tatcaggcag gaacaacaga cttgctggtc acttggctct ataaattaac cgtcacagct
     3781 gctgactaca atttagcttc tgttatcggt atcttaatct ttacagtttc agctatcttt
     3841 agcttacttg cttatacaag gacagcatcc tacaaggaag gagcggctaa ataatgaaaa
     3901 acaaacgtcg atttcaactt ggacttgttt atgctacttt aatcatcctc tcaatcattt
     3961 ggctcttccc aatcgcttgg gttattctta ctagcttccg tagcgaagga actgcttacg
     4021 tcaattattt tattcctaag acttttacat taaatcacta cattaatctt tttactaatg
     4081 aaacctttcc ttttggaaaa tggtttatga atactcttat tgtagccact tttacatgta
     4141 ttatttcaac cttcataact gttgccatag cctactctct tagccgaatt aaatttaagt
     4201 ttcgcaatgg ctttttaaaa ctagccctta ttttaaatat gttccctggt ttcatgagca
     4261 tgattgccat ctactatatc ttaaaggcct taggtttgac gcaaacccta acagcacttg
     4321 tccttgttta ttcctcaggt gcagcccttg gtttttacat tgcaaaagga ttttttgata
     4381 ccattcctta ttcacttgat gaatcagcta tgattgatgg cgctactcgt atggatattt
     4441 tctttaaaat aacccttcct ttagcaaaac caatcattgt ctatactgcc cttcttgctt
     4501 ttatggggcc ttggattgac tttatctttg cacaagttat tttaggtgat gctacaagta
     4561 aatataccgt tgctattgga ctattttcaa tgcttcagcc agataccatt aacaactggt
     4621 ttatggcctt cactgctggt tctgtcttaa ttgccgtccc aatcactctc ctcttcatgt
     4681 ttatgcaaaa atactatgtt gaaggcatca caggtggttc tgttaaataa gttacaacat
     4741 accaaacctt ctaagggcaa gccctttagg aggtttttga taaataaggc cgtagataat
     4801 catattagtc ctcacattta tcttcagatc agccaaatgt attaatgatt ccttgacttt
     4861 taccatgaat tcggatataa caaaatgatg acatctaaaa ttaccattaa agatattgca
     4921 cgagaagccg gggtttctgt ggcgaccgta tcttacgtgt taaaccagcg caatgatcaa
     4981 aaaatcagcg aagaaacccg caataaagtg ctacatatta gtaaccttct taactataca
     5041 cctaatcagg cagccaggtc attagtcaca caacaatcaa acagtgttgt cctgtattac
     5101 aaatatgcag atagcatttt agctagggct gaacaactgc ataccattag ctttctatcc
     5161 cgcctattgc atgagcagga ctatcagctc gtttgcctta atgaagatag tattggaaaa
     5221 tttgactggg ctgaccttat tctgacctta gatattgaaa atgatacctt ttacaaaatc
     5281 gggaaactta atttcaagcc cttaattgct attgatagct ttgttaatga ccctctcttc
     5341 ttccaaatta acacagatta cgcaaaccta aaagaccaag cagatcagtt ttttaaggat
     5401 gaaccttata ccctgctgac tctccctatc aacaatcaag aacggtatga ggctatctcc
     5461 accctttttc caaacctaca tgtggttgac cacatgaggg agttgcctag ctttgaaaag
     5521 aaaaatcttt tactcattga tgagagtttg gaccaattct ttgtgaactc ttcgcaaaaa
     5581 ctctactgcc cacgtttttc cccagaaaaa gggcaagtta tcttagaggc catcgagtgg
     5641 gccaaacaac gacaactcaa caaaaatcat aatcttctcg tctaatagca cacaaaaccc
     5701 cttgcacttc aactgaagtg caaggggttt tgatatgaca tcggtgtcag agttctctta
     5761 ggatggtaac gaatctctaa actgtgtttt gtagaacacc caggtcagca ttaataaggt
     5821 accaaatact tggcaattca taatcaatac gggatcctga ataaagaaac taagagcaat
     5881 tgccaataga cttggccagg ccaaacaatt gaccatcaaa ccaagacctt ctgaaaaatt
     5941 tcgaatcata aacagccgtg atcgtttggt caaggtcaaa aagaaagcgc ctagaccaat
     6001 cacaataagg gtgcccacat acaccatgag aacaaacgtc aacagaagaa aaccaagtac
     6061 actggcttta ttactgtcat accattgctg attgacaaag tccttcactg ctttaactgt
     6121 tgtgaggtcg gcgagcgact gctgctggcc tctaacgtga gcacggagtt ccttgccttc
     6181 aggggtaaca aaacgccatt ctttttttgt cacaataatt tgcctggttg attctgatgc
     6241 caatttctct aaatcgacct ttgtcggtaa gacagccaaa accaattcac catctcgtcg
     6301 aatcagagat tgaccctgaa aggtattttc ctcaaccttt ccttgggtca atgcgtgata
     6361 ggttttgtct gtgataggcg acaaactctt agttacaatc cgttccaagg gatacgttgt
     6421 catgttagca tagtgtaacg taactggaat cattattaag gcgttcaaaa aaacaataat
     6481 aagaaggttt tgccaccaag taaactgagc tcggctggca aacattgccc taggagaaag
     6541 gcaatgtgaa atgtaagaaa taggaaattg ttttttatct ggcattaaac attactcctt
     6601 gtcaggttac cctttggtac caccacttgt taaacctgag acgaaatttt tctgtaggaa
     6661 gaaaaagaga actgaaatag gcactgcaat cagaatagct cccgcagcaa ataaggttac
     6721 cttaggattt ctagcatcat tcgtaataaa cgattgaaga ccaactgcta ccgtataatt
     6781 ttcttgcgcc cgtaaaagga atttagccaa catgaagtct ccaaaaggac ccataaaagc
     6841 ccataaggat tgtactgcaa tcattggtcg cacaagtgga aggacaattt ggtagaaggt
     6901 cctaaagtgt ccagaaccat ctaacttagc agattcgtcc aagtcataag gtaccgtgtc
     6961 aaaataaccc ttcatcaacc aggcattcat tggaatacca ccaccgacgt aaatcaaaat
     7021 caagaaccag tattggttta aggcattaaa gagccaagcc ataacaaagt aagccgttaa
     7081 agccgccatg gtaggaacca tctgtacgac taggaaaaag attaaacttt ttttgcgtcc
     7141 aaagaaattg taacggctat atgcataacc tgtcaaagtg atgactgtta cttgtatgag
     7201 catggtgaag aatgccacga ttaaggtatt ccaataccat cttaagtata aggtatcttg
     7261 aaagagtgtt ttaaagttag acaaggtcca tggcccatca aaatgaaaac taaaggctgt
     7321 ggtatttcct ggtctaaaag ctgaactgac cgtcactagt atcgggaata aaatcacgat
     7381 cgctaaggag attagataga gataggtcaa taattgggtg aagaaacgtt tgcgtttcat
     7441 ggatgttgaa acataggtct ttctcattat ctctcctcca tatcaaaagc tttaaatttc
     7501 ttaaaggtaa tcaaggatac cgaaatcact attacagaga tgattaaggt tactgccgac
     7561 gccatcgaaa actgaggtga ggtttgtgtg gtcaacttgt aaatccagga aatgaggata
     7621 tcagttgtcc cagctccgcc cccaacagta ccaggccccc cattattaaa cagataaata
     7681 atagagaaat tattgaagtt aaaagtgtac tgtgcaatca gcgtcggtgc tgcaaccgca
     7741 aaaatcattg gcatggtgat atgacggaat ttctgcaaga ctgtcacacc atcaacggtt
     7801 gctgcttcgt ataaatcagc tggaattgcc tgtaaaatac ctgataccaa aatatagata
     7861 tagggaaatc ctagccaacc ttgcaccata ataacagcca cttttgtcca gaaggggtct
     7921 gtcttccagg acacaattcc gaagtcaacg aaaggaagga atttctcgat gaaaggaaga
     7981 acctgaacat tcatggcccc aatggaatca ttaaagaaat taccaaaact cataatagag
     8041 ataaatgctg gaacagccca tggtaacaag aatatcacac caaagagacg ttttcctctg
     8101 ataaaggatt gattagcaat aacagccgtt gcaattccaa taacaatttg aagacttgtc
     8161 gctgctaatg tccagatcag cgtccatgac aaaacagatg taaatgctgt tttgaaggtg
     8221 cttaattcta agatattaag aaaattcttc aaccctaccc aatccaataa acgatacgga
     8281 ggaatatgcc taaaatcgta attggtaaaa gcaatcagca aggtcactaa aactggaaaa
     8341 ataatagcga aagccattgc taaataagcc ggaatgatga gcaagtaagg aaatcctttt
     8401 tcataaatcg cttcaagggt ctctttagcc gtcaccggaa ccttcttgcc ttcattcatt
     8461 tgatgtgcaa ctaatttagc atctctcata gcaaccaaat gaaaaatacc aaataagact
     8521 agagtaatta attgcatggc ccccttaatt aacataaata aggagtgatc ttgcattgga
     8581 acatttccaa gactgtaaag acctgttaaa gcaggaattc ctagaactac aatttctagt
     8641 agctctagga agaaaacaac taataagact ccccctttaa cgatttgttt attgtatagt
     8701 tgtcccaaac ctgggataac agatagccac atggctttat taggcttctg ttgtctttgg
     8761 ttatctacca ttttttattc ctcttctcat aaatgattta aggttatcct ccttatcata
     8821 ctaacttcct tttaagctga ctaatttatt tttttatagt aatggtttgt gcttcatgac
     8881 ctgtctttat actataagtc tcaggacttg tccataaaac attcccaata ctatcttttt
     8941 taacaagttt gactgctatt tctttattga ttggtagatg agtatcaaag aaccagtttg
     9001 ggtacttggc aatggtctga gtgttattaa atagaggacc aacagcattc ttagcgtcat
     9061 ttgcccccat ctcaaaaaca tcacccatga gatatagttg ttcccctggt actgtcttaa
     9121 aatcattgat gagtaaacga acaggaattt gtttatcagt caatacttca aaggcttggt
     9181 aactattgct ggtttgctta tcggcagttg tcactgaaat gttataataa tttgctggaa
     9241 ccgtcggtac ttttaaggtg ataagggtat ctgaccaaga aaggatctca gcagagattt
     9301 ctccaaaagt cacttgacct ttagaattac caaaaccttg acctgaaata gtaatcttat
     9361 ttccagcaat acccactgaa gcatcgacat ctccaagttg tggtgtcttg tcctctcctt
     9421 cataagtcca tacagcgact tgccccgctc caagttcaaa gctactaata gtgccatctg
     9481 ctccaactat taattcttga ccgccaagaa gaccttctaa tttgtccttg tagctatttt
     9541 ggggcaaggc tgttttagca ttagtaattg tataaccatt cgtttgatct ctattaatag
     9601 ccactagtgc gacatgatta ccaaatttac gctcaaatac caaaacatga tcactaatcc
     9661 aacgttgttc agttgttcca taagctaaag cttgattttg ttttcttaaa ggagctagct
     9721 tactaatcac tttataggct tgtgactctt tattaaaact tggcatatca ccacgattat
     9781 taggatcttt atcacctgtt gcatactgct ctgtaccata ataaatatta ggcacacctc
     9841 tagatgttaa aagcaaagca taggcttgat taacagcagt ttgattatta gccacttttg
     9901 ttgcgaagcg ttccatatca tgattatcaa taaaggtcac ctggtctgtc acttcattag
     9961 tcacctgatc tctattttca agaaccttgt aaaaatctcg catggtaaac gttgaaaaac
    10021 ctgtatacaa ctgtctaata gcattggcaa atctaaaatc taaaagcccc atcccactat
    10081 tgttagcaaa tgtcgtcata tcataatcat cgtcggtatg tcccgagaac cattccccga
    10141 aaacaaagac attatgtttt tcatagatat gactcaacca atttttttgc caaccttgtg
    10201 acatatgttt aacagcgtca actcggatac catcaacacc caggtctaac catttatcaa
    10261 tagcttcttt catgtactgg tcaactttcg gattaatgtt atttaaatca gctagtccgt
    10321 acattgaatg ataaatagaa ttttcataag tactaaaatc ggtccaagat tcatgattaa
    10381 aaatcttgtc tttatcatct gaaaatttac caactaattt accgttttta tataaagcgc
    10441 catcttcttt gaaagttgtg ccttcttttt cggctgtaga cgtatgatta ggagcaaaat
    10501 caataactac cttaatatgg tgttgatgag ctactttgac tagttgttga aagtctgctt
    10561 cagtgccaaa atgctggttt gttttaaaga agtccttagc ccaataccca tgatatgcag
    10621 cacttccatt agaaggatca atactgtcga tattttcaac aggagaagaa atccaaatgg
    10681 cagaaatccc catatctgtc aggtaaccat ccttaatctt ggcgatgatt ccttgccaat
    10741 cacctccatg gtatttttta aggtcatttt tatcaaaaac atcagtctta ccattattag
    10801 aggtattacc atcgttaaaa cgatctgtta caatctgata aatcgtatct gttgaaaaat
    10861 ctgctttgtt ggtgacagaa gcattatcgg cagcagaaac agttagtgga aatgaaatta
    10921 agccaagtaa aacagcactt ttcgttaata acttataagt tttgatatgt aattctctca
    10981 tcgctttttc cttttcaaat aaccttttag ttactaaaca caatatatcc atcctggagt
    11041 aattgttttt tgtttttagt taccccattg cttgtcaaaa tctgtcctcc ttccttaata
    11101 gaataagcac tctcaccatt gttaagaaca agtgaaatgc tttgctcacc taagctgcgt
    11161 ttcagaatga ggcagtcttc gtcataatca atgctaatag ctccttcttg aagagctggc
    11221 tgttggcgaa gagcaataat cgcttttact ttgctaaaga aatccttgtc ccgctcagca
    11281 tcatcccaag gaaaaccgcg acgcgaatca gggtcgtaac ccccttccat tgccacttct
    11341 gtcccataat acaagcaagg aatgcccata aagataaaga gcaatgctaa agcagctaac
    11401 aatttatctt tggaaccctt aacttgagtg aagaaacgat gcgtatcatg agaatccaaa
    11461 agattcaagt tcatgcgatt gacttgccaa gtgtttcgca tcaaaatgtt gcttaatttc
    11521 tcagccaatt gttttgatgt catggtttca aaagcaaagt aatccaaaca ggctttggtg
    11581 aaagcataat tcattatccc atcatactga tcccctgcca aataaggata ggcatcatgc
    11641 cagttctccc caattaaaat ggcatctgct ttttccgctt taaccgcctg acggaaacgt
    11701 cgccaaaaat catgagatac ttcatcagaa acatctaggc gccaaccatc gatacaaaat
    11761 tctttaatcc agtatcgccc aatctcaatc agatagtctt gcacgtctct atttgaggta
    11821 ttccatttgg gcatatagtt acatccagca aaggtttcat agtttactag gtccatggat
    11881 gggtgttcat catgagtcat aaaccaatca aaaaacttag actctttacc gtatctcaag
    11941 acatcctgaa attccacggc atcactgctg gcatggttaa agacagcgtc tagaataatc
    12001 ttgattccca tttggtgagc taaatcaatc aattcttgca aatcatattt agttccaaat
    12061 tgtggatcaa tagcgtagta atcactaata tcatatttgt gattactaat ggattgaaaa
    12121 attggcgtta aatagatgac agtaatccct aaatctttga ggtaatctaa tttttctgta
    12181 attcccttta aatctccacc agcaaaactt ttaggagtcg gtttttctaa ccaacccatg
    12241 ttgacataac tgtcatcttt ttcccaatcc ccacggttaa agcgatcaac aaagatttga
    12301 taaaaaaccg cgtcttttgt ccaaggaatg acttgatgga catcaacagc attgatataa
    12361 ggcatttgga agaaattata aaacccattt gaaaaatcgt aactagtagt taacccatct
    12421 tcactaaaat aataggtttg accttcttct tcaattttaa aaatgtaggc caacctcaca
    12481 tcgtctaatg ttaaagtagt ttcataataa gcatgcgtat ggtctgtata ggcaactgac
    12541 atttccaatt cttcttggaa ttcatgataa ccatatttag gcccgtggac caaattaaca
    12601 gtaatacctc tatcttcttt ggccacgcgt aatcggatga caagttcctt ttgagatatg
    12661 acaaagcaat acctcgaatc aggaatatgg agcattccgg caacattcat ttctacctcc
    12721 attaataaag tattgacgat tacttctttt tctttaaaac gaaaaaatag cagagcgact
    12781 catacttcta tatttttagt atctgtttca tcttcatcaa actaacagaa atagaagctg
    12841 ggcgtacttc tttatcccag cctcccttct ttgccttatt cagcgtattt ttgttcaata
    12901 gcttccttaa tagtttttgc tgcttctttt gcagctttag atgcttcttc cttaccagaa
    12961 gccacgttaa agaacatatt tgcacctggt tcccaaactt cagccatttc tggaatattt
    13021 ggcattggtt gtgcgttctc aaactgacta atcacagctt tcgttaactc attaccctct
    13081 ttagaagcat agttacgtgc tgttaagtta gctggaattt cttgtgtttt atcatagaaa
    13141 cgtttttggt tttcagcatt ggtcacataa tcaaggaatt tttgagcagt cgtttttcct
    13201 ttagaatagt ttgaaataac ccaagcttta ccacctgcga acggttggta ttttttacca
    13261 tttgtcaaag taggaatttc cattacccca aagttaacac cagcatcttt aaaggagcta
    13321 gctgcccatg gaccatcaat aatgacacca gcttttttag aaataaattg ttcagtgata
    13381 aaatcaccag cttttttagt gtcttgcatt ccttgaggcc aagtgccata ccactgtttc
    13441 gcgtagttta acccttcaac agttccgtcg ttacctaggc ctaaatcact tggtttggtt
    13501 cctttatcac caaagatata accgccataa ccggcaatta aaccataacc ataatagaag
    13561 tctgtccatt tcgccaagaa acctacagat tttcctggtt cagaggcaaa agcaaactta
    13621 gaatcttttt gtaagacttc taattcagta aagcttttag gtgcttgagg cactaaatcc
    13681 ttattataat aagttaccaa agtttcaatt acgtctggtg caccataagt tttaccatca
    13741 atggttacta atttcttaac cgtatcatca aactctttgc tgtttcctaa agtaacctca
    13801 gcgatttgac cttcagttcc taagccacct acacggtcaa aaggtgctaa aaagacatct
    13861 ggtgaagctc ctgttggacc atccgttgat agcttatcaa gagtatccat cataccttct
    13921 tttttgactt taacggtgac tttatgttct ttttcaaact cacccttaat actcttaatg
    13981 taatcaacat atccctcatc aacagagact acaatagtct ttccgtctgt ttccttaacg
    14041 cctttaccac caccacttac gcaagcagtt aggggtagca ctgaagctaa ggataaagtt
    14101 ccaagcacag atgttttttt ccaattccat gacataacat gttcctcccg aaatggtttt
    14161 ttcttaaaca caaatcaaat catctatcta ctctcatcat attagcaaat aaaagataac
    14221 ataaggcatt taaaattaat aacagtaata gtttcgaaac ttaaacgttt aagttaagtt
    14281 tatcgtaatc gctttcattt gtcaataact tttttacttt tcttttcttt taagagcata
    14341 tcagtagttt tcctgctaat tgaaaacaag gcatttactg tcacacaagt ctgtcctttc
    14401 tttttttcta caactttatt tcgtcagcgt aaataaaaca aaagctctat aatctcttga
    14461 atgggtaaat ccactgtaga gattatagag ctttttgaat atcaaaaaag tcccataaga
    14521 tttataatga aaagcaacca aactctcatt agaaagactc ttatggaaca tatcaatcat
    14581 accttacaac acatcggaat taaagacaaa aatatcactc taaacaatgt tttgaaggat
    14641 gaatctccta ctgagctcgt tactacttat cagaaaggta gataggaaat cctgatgaag
    14701 agtgtgtcca agggattatc aattaggtct tagattacaa aatagtaatt caggtatgct
    14761 ctactgtttt caacactagt tctgcaattt gttttcgagc ttgcttagct tctcttattg
    14821 gaaaaataac atacgcatga atcatttttt ctgcaataat cagctgatta gcaattcgtt
    14881 gtcttgttaa ttgatcatgt aaagacataa tgtctgggta aaaaatctct tttgtcccaa
    14941 caaaggtcgt tatttcaggc aaatcctcat aatcaccata caaaggactg acataaggaa
    15001 gttgcatatt accttgacca cctgcccata gttcacctat atcacgaaga cgacgagcat
    15061 ctaggtaagc ttcttctgct tcaaaatcct tgtaggcttg atttttaaaa ctaacatcta
    15121 accaaggaga tataagaatt aattgctttg gctggggtgc tgttttagta tctcgtatat
    15181 acttagcaaa cgccaaagcc atacccccac cagaagaatc accaatgaca ctaacttgac
    15241 tcggtccatt agacaagcaa aattgctggt aagcttcaac taatttagga tagacatctt
    15301 gataagtagc atacggtact ttaggaaagt taggaaaaac aaccttggca ttagtcagct
    15361 gtataagatg cttaaccgtc ttaaaatgaa gtttttcggg tcgtcgtaaa tagacactgc
    15421 cataaagata ataaataaca ggctgatgac tttgcttttg atcattaata ataaaaactt
    15481 gcatcccgtt cacatcgtat tcttgagttg aaaaacctac tatctcttca ggattatcta
    15541 gatgataagg gatattacga ttacgctgcg tctcaacccc cttataatta tcaacttttt
    15601 tagataactt atactttcta taagcaaaca ttttctcaac caaccaactt gaaaaggagc
    15661 gcttttgacc atattcaact atcaaaatac ctagtatcac tccaaaaaaa atcaccacag
    15721 caataatgac cacttattat cctcgttttc taatattatt ttactattgc aagtctttcc
    15781 tatttgccca aattttacta taaaaataag ggttcatttt atagttgggc actggctgct
    15841 tcgtttctag aaatggttgc actttcttat caaaagctaa ccagccattc caaccgagat
    15901 ggatggtatc ttgcataaag taggactcac caccatcttt tgagaagtca gcaatgcgat
    15961 gaaatccttg tgactttaac tggaatttta ttttacgaac tgccgcttga tacttatctt
    16021 gatttaagcc ggtataatcc gcccaagctt tattaacagg agttataaca aagagtacat
    16081 ctgtttttcg tttagcaaat tctgataata aaagctgaaa atcattgtat tctggtgacg
    16141 ccaggtaact ataattaact tggaaattct tataaagatt gtatttaggt gctattcgtt
    16201 tacgataaaa tgtattttta atcccaaaac ggttgttggt tgttgctaat tggcctctct
    16261 tagttgctaa tgcattcaat tgtttatacg aaaatacttt aggtaatccc ttaacgcgag
    16321 gcaaaattct tttttcatag ttagtagatt tgcctaaaaa actaaaaagg gactcttctc
    16381 tcaatgctac ttgatgttga catttcaaaa tagctctgtc taaccgacta agagacttac
    16441 ccttacttac ttttttcaat aagtttgatt tagacacacc agggttaagc tcaagcaaac
    16501 gctttgctgc aaactgtgat tctttatcag ttctagcttt tagtaaaaat tcaatcactt
    16561 gagtgttaga caagtacatc tgaaccgcac taggattaat cccttgagca gtaaaccatt
    16621 gaggagatac tacaaagatg gcttttttct tttgcatttc attggtaatt tgttgcatac
    16681 cataataatg cgacaaagat gctgatcctc tcttaccaat taaaaatggt ctatagctcc
    16741 gcttgtagcg ctctgcaagc accgaagggt gcatactatc cattcgactc cattcgctag
    16801 aaccaaaaaa aggcacaaaa cgacaagtct catctgataa agcttgtctt ttaatcaaac
    16861 catttttaaa agaactatct gtgatcgcaa cggcatttgc tttttcctga gctatggaat
    16921 gatcaagttg tgtgggaaaa ctaaaaatag taatcactac taaaacaaag gcaataagaa
    16981 gaggacctag aattaaccag agtctcttaa gcatgttgca attcctctac accttgaaca
    17041 atcttagtta ctgtgttcca atcatcacga ccaaattctg aaataggtac tttaatattg
    17101 aacgttgact ctaactcaac aatcaattcg actgtcccaa gactatccaa cacacctgca
    17161 tcaaacaagt cttcgtccat catctctgaa acatcttcca taaacaaacg gtcaaacaat
    17221 tcaattacag tttcttcaat agacattttt atacttcctt ttattaatgt agtggtctgt
    17281 taaaccaaag gtcatttaaa aaaccagaga ataacagaag tgaaaacatg acaacgtgga
    17341 aagttaccac gatacataag acatggaatg ccctgctttg aaagaggggt gataagcctt
    17401 ttttcttacg gtgacggttg atttcttttt tcttacgaat ccaagcatca ttaataataa
    17461 gtcctatacc atggaaaagc ccataagcga tataatacca ggttaagcca tgccaaaatc
    17521 ccatgactag catattaacc agataggcaa cacctgaagt gacatttcgg tttttaaagg
    17581 ttttatgctt aatcaataga tgaaccaatc gcataaacac ataatctcta aaccagaagg
    17641 acaaactcat gtgccatcta ttccaaaaat ctttcaaact tgcagataag aaaggcatgt
    17701 taaagttttc tggtgttcga atacccaata ggtaggaaat accaatcgca aacatcgaat
    17761 aaccagcaaa gtcaaaaaag agattcaagc catacacgta cataaccaga atagtttcct
    17821 tattaaagta gccaccaacc ataaaagcct tattttcaag gagaggaagt aagatgccac
    17881 ccaaacaata ggaaatgata tgcttgtata gaaaacctaa catgagatac atgactgcct
    17941 tattgagcat tgcaaaataa tcatcacgtt caggaagttt atgataatct tcctgaaaac
    18001 gtctgaagcg atcaatgggg ccacttgaga aagtggggaa gaaaatcatg aagcgaataa
    18061 aatccggtaa acgaacctct tggagagtcc cgtcacgcat ctcaataatc attccaatac
    18121 ttttaaaggt caaatatgaa atccctagaa aactaaagag tgtttgagaa gaaggacctg
    18181 ttaggggagc aactttgacc cagataaggg ggaataaggc catcgcaata gctaagtaaa
    18241 acactcctgc actattagct ttctcacggt atttctggta agcaaaaaca ctgatgattt
    18301 gccacaaaag ataaaagaga aaagcaacta attgttgata atgatctcct ccaaacataa
    18361 agagaataaa taccaaactc accaaggttt cataagtgct taagcgcttt tgtttaaaaa
    18421 tcccaatgat gactggtaag aaagccaaaa taagatagac aaagtaaatg gggttaccgt
    18481 aaggttccat gtagggaatg tggctaaaaa acatcatcat cggttattga cctcattaat
    18541 caaagtttta atatctatct taccatttgg agttaatggt agactatctc gataaagaaa
    18601 cttagaaggc atcatataac tcatcatatg atccttaaca gaggctttga tagctttggt
    18661 taactccaat tccctatcaa aacgctcttt aaccccatct ttgacaacga tataggccaa
    18721 gagattttga accttatgtt ctttattgta ccttggaaca gccacagccg atgctaccat
    18781 tggcgattga ttcaactgtt gggaaacatc ttctagttca atacgataac cagcatattt
    18841 aatctgaaaa tccaaacgcc caccatacag caaaatatta tcttctgtca atgagccaat
    18901 gtcccctgta tgataagcag gctgtccttt aaaagtaaag aaagcttctg cggttttctc
    18961 aggattgttg aggtaacctt ttgacacagc aggccctgta acaataattt caccctgctc
    19021 ccctgaagat aactccttac catcttcatc aataatatag gtcggagaat ctggtttggg
    19081 gtagccaatc ggcaagcggg tataattatc caccatttct ctggtaattt caatagcgga
    19141 taaggctaca gtcgcttctg ttggtccata ggcattgata atcttggcac tcgggaaacg
    19201 ttcaaagagt tttcgagccg tagaaacggt taattcttcg ccatcaaaat aaaaatgcgt
    19261 taaagctggc atcttagcct ggcaaaaatc atcgctaagc atggccatat cagcaaatga
    19321 aggagtcgat gtccaaatgc caactggaag ttgcgcaatc gttgtaaata actgtttaaa
    19381 gtctgccacc aattcttttg gaagtgcaaa taaggttcca cctaaagcca aggtaggtgc
    19441 ccagtacatg acagaaaggt caaaggaata gggtggctgc gccagcattt gtggctgttt
    19501 aggtacatca aatgctgcat cttcgatcat ccagttggta aagctcaaaa gattgtcatg
    19561 tgaaatttgc accccttttg gttgacccgt tgttccagag gtgaaaataa tgtaataatt
    19621 atcatctcct ttaacagaat gtgttcgctc atagggcatt tctgctaatt ttgctgactc
    19681 aatctctgat agtgagacaa gggaaatccc ttcaatagta agagggaatt cctcaatagc
    19741 aatgattaaa ctgggctttg caatctcaat aatcgctaaa atacgttctg gtgctgaatg
    19801 cacatctact gggatatagg catgaccaga tttggttaaa gctacaaaag tagctaacat
    19861 atcataagtt tgcgccccaa aaaccaaaac tggagatttt gccagtaaag ctaagctatc
    19921 tataaatgca gcaatgctat cagaatctct tttgagttgt ccgtaagtga ggcgttcccc
    19981 taaacaatca taaactggaa aatcagcctg tgtctgagca aactgctcaa tactgtcaat
    20041 catatcctta atcatcagtc aatctcctaa aattcattat aaataaagtt actttgacca
    20101 tgtcctagat aaccaaagaa atagaccagc aacattaaaa tcagataaaa taaaattgtc
    20161 ttgccaataa aaacagcaat tcctctcatt cttgatttgt tttttatcat actttacctt
    20221 cttgtattag tgaaataatg tcctaaaaaa ttctactcga aaaaacctaa cttgacaagc
    20281 taaaagtcat atta
//
LOCUS       DAUGFP010000029        18122 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.029, whole genome shotgun sequence.
ACCESSION   DAUGFP010000029 DAUGFP010000000
VERSION     DAUGFP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18122
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.029"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            541..930
                     /locus_tag="VH663_001098"
     CDS             541..930
                     /locus_tag="VH663_001098"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEQ8174306.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
     gene            1024..2076
                     /locus_tag="VH663_001099"
     CDS             1024..2076
                     /locus_tag="VH663_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ8174307.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            2215..3747
                     /locus_tag="VH663_001100"
     CDS             2215..3747
                     /locus_tag="VH663_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174308.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVADRVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGVPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNNEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVLPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            3740..4804
                     /locus_tag="VH663_001101"
     CDS             3740..4804
                     /locus_tag="VH663_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174309.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            4806..5762
                     /locus_tag="VH663_001102"
     CDS             4806..5762
                     /locus_tag="VH663_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174310.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(5980..7698)
                     /locus_tag="VH663_001103"
     CDS             complement(5980..7698)
                     /locus_tag="VH663_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ8174311.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENTALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
     gene            complement(7874..8443)
                     /locus_tag="VH663_001104"
     CDS             complement(7874..8443)
                     /locus_tag="VH663_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8174312.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEMIISAIITFVLIPTLSRLKR"
     gene            complement(8501..9046)
                     /gene="coaC"
                     /locus_tag="VH663_001105"
     CDS             complement(8501..9046)
                     /gene="coaC"
                     /locus_tag="VH663_001105"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEQ8174313.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            complement(9039..9731)
                     /gene="coaB"
                     /locus_tag="VH663_001106"
     CDS             complement(9039..9731)
                     /gene="coaB"
                     /locus_tag="VH663_001106"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEQ8174314.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNKRLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            9912..10763
                     /locus_tag="VH663_001107"
     CDS             9912..10763
                     /locus_tag="VH663_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEQ8174315.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDRNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            10760..11959
                     /locus_tag="VH663_001108"
     CDS             10760..11959
                     /locus_tag="VH663_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEQ8174316.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVINQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNIAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            11956..12954
                     /locus_tag="VH663_001109"
     CDS             11956..12954
                     /locus_tag="VH663_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEQ8174317.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEEAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            12996..13328
                     /locus_tag="VH663_001110"
     CDS             12996..13328
                     /locus_tag="VH663_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEQ8174318.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            13321..14133
                     /locus_tag="VH663_001111"
     CDS             13321..14133
                     /locus_tag="VH663_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEQ8174319.1"
                     /translation="MPSSFDLLGEMIGLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGHHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEAAQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKALYDTYLQKENNCE"
     gene            14126..15007
                     /locus_tag="VH663_001112"
     CDS             14126..15007
                     /locus_tag="VH663_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEQ8174320.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILETKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            15054..16073
                     /locus_tag="VH663_001113"
     CDS             15054..16073
                     /locus_tag="VH663_001113"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ8174321.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KSTYQPAIEALHHLGATGVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     DLIFS"
     gene            16201..17871
                     /locus_tag="VH663_001114"
     CDS             16201..17871
                     /locus_tag="VH663_001114"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ8174322.1"
                     /translation="MKSDIEIAQSVALQPITDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLILVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFAEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            17956..>18122
                     /locus_tag="VH663_001115"
     CDS             17956..>18122
                     /locus_tag="VH663_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEQ8174323.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVW"
BASE COUNT         5529 a         3120 c         3793 g         5680 t
ORIGIN      
        1 aacctttttt aatgtatttt aactgaaaaa ggctttcata attttaaaaa aatgttaaaa
       61 taggtgtata acaactgatt accaagagat tattatcatt atcttctaag gtgtcattgt
      121 tatggtgaca atcaaccata cgctactaaa gataggtagc gttcgatcac agattgatca
      181 agtgattgat acagatattt ataattttac aaatatctgt atcaatccat gttaaatagt
      241 ctatgcttct aaggtgtcga cgttaatgta ctgacgggac cgttatagtc tttctttttg
      301 gagaaacgtc ggataccagt gaactagttg aagttaaaga tgttaattca aacagtgctg
      361 aagagattga tatcgttttg aacggtcgtt tacttcaaag caaagaaatt accagtctat
      421 cgaagatgat ataagtgcta aagttagatc acgttaatta tctatcaagt cgggtgcata
      481 tttcgttaaa gtatctacag gggtttcaat agatgtagct attatgaaag gtgatgttaa
      541 atggggacta ctgatttagt tagttgtgct gtacaagcta gtgagtatgc ttatgtccct
      601 tactctcatt ttccagtagg agcagcttta aaaacaaaag atggaacgat ctatactggc
      661 tgtaatattg aaaatgttag ttttggtctg acaaattgtg gagagcgaac agctatattt
      721 aaagccatat ctgatggtca taaagaattg gttgaaattg ctatttatgg agaaactatg
      781 caacctgtat ctccttgtgg ggcttgtcgt caggtaatgg cagaattttt tgctccttct
      841 tctttagtca ctttaattgc taaaaatggt caaactgttg aaatgactgt tggagattta
      901 ctcccctatt cttttacaga tttaatataa tctgctttaa tttctgttat ctagccgtct
      961 tggctttata tttactttca gcaacttatt tgctagaaat cttttttagg agggttcata
     1021 ttcatgaaca agaaatttat tggtcttggt ttagcgtcag tggctgtgct gagtttagct
     1081 gcttgtggta atcgtggtgc ttctaaaggt ggggcatcag gaaaaactga tttaaaagtt
     1141 gcaatggtta ccgatactgg tggtgtagat gacaaatcat tcaaccaatc agcatgggaa
     1201 ggcctgcaat cttggggtaa agaaatgggc cttcaaaaag gaacaggttt cgattatttt
     1261 caatctacaa gtgaatctga gtatgcaact aatctcgata cagcagtttc aggagggtat
     1321 caactgattt atggtatcgg ctttgcattg aaagatgcta ttgctaaagc agctggagat
     1381 aatgaaggag ttaagtttgt tattatcgat gatattatcg aaggaaaaga taatgtagcc
     1441 agtgttacct ttgccgacca tgaagctgct tatcttgcag gaattgcagc tgcaaaaaca
     1501 acaaaaacaa aaacagttgg tttcgtgggc ggtatggaag gaactgtcat aactcgattt
     1561 gaaaaaggtt ttgaagcagg agttaagtct gttgacgata caatccaagt taaagttgat
     1621 tatgctggat catttggtga cgctgcaaaa ggaaaaacaa tcgcagcagc tcagtatgca
     1681 gcaggtgctg atgttattta ccaggcagca ggaggcactg gagcaggtgt atttaatgaa
     1741 gcaaaagcta ttaatgaaaa acgtagtgaa gctgataaag tttgggttat tggtgttgac
     1801 cgtgatcaaa aagacgaagg aaaatacact tctaaagatg gcaaagaagc aaactttgta
     1861 cttgcatcat caatcaaaga agttggtaaa gctgttcagt taatcaacaa acaagtagca
     1921 gataaaaaat tccctggagg aaaaacaact gtctatggtt taaaagatgg cggtgttgaa
     1981 atcgcaacta caaatgtttc aaaagaagct gttaaagcta ttaaagaagc gaaagcaaaa
     2041 attaaatctg gtgacattaa agttcctgaa aaatagtgtt taagaacaag aagcgcgacc
     2101 tttgtcggtc gctcttttta aactgagata atttttatct cagtaaagtt tgtgataggt
     2161 gatagatcat aggctttact gaaataaaag attgcattga aaggaaatga tcctatgaca
     2221 cagcatgtca ttgaaatgag agaaatcacg aaaaaatttg gtgattttgt cgcaaatgac
     2281 cacattaact taaatgtcag aaaaggtgag attcatgcct tattgggtga aaatggggca
     2341 ggtaaatcaa ctttgatgaa tatgctagct ggtctcttag agcctactag tggagaaatt
     2401 gtcattaatg acaagccagt tcaaattgat tcgccatcta aatcagctaa gcttggtatc
     2461 ggaatggtac atcaacattt catgttagta gaagccttca cggttgctga gaatattatt
     2521 ttaggaaatg aagtagtaaa aaatgggtgc ttagatctta accaagcatc gaaagatatt
     2581 aaggtactat ccgaaaaata tggccttgca atcaatccat cagctaaagt ttctgatatt
     2641 tcagtaggag cacaacaacg tgttgagatt ttgaaaacct tgtatcgtgg tgcagatatt
     2701 cttatctttg atgagccaac tgccgtttta acacctgcag aaattaaaga attgatgact
     2761 atcatgaaga atcttgtgaa agaaggaaaa tcaattattt taattaccca taaactggac
     2821 gagattcgtg ctgtcgccga ccgtgtcaca gttattcgtc gtggtaagag tatcgaaaca
     2881 gttgatgtgg caggagcaac ttcacaagat ttagctgaga tgatggtggg acgctcggtt
     2941 tcctttacaa ctagcaaaaa agctgctgaa ccaaaagatg tggtcttatc catcaaaaat
     3001 cttgaagtag atgaaaatag aggggttcca gcagttaagg ggctatctct agatgtccga
     3061 gctggtgaaa ttgtagggat tgctggaatt gacggaaatg gtcaatctga gttaattcaa
     3121 gctattacag gtttacgtaa agtcaaatct ggaagtatta tgattaaaaa caatgaagtc
     3181 actcatttgt cttcacgcaa aattactgag ttatcagtgg gtcacgtgcc agaagatcgt
     3241 catcgagatg gtttgatact agatctttct ttagctgaaa atacagcctt acaaacctat
     3301 tacaaacaac ctctaagtca aaatggaatc ttaaattata ctaaaattaa tgactatgct
     3361 cgtcaattaa tgaaagaatt tgatgttcgc ggagccaatg agctagtacc agctagagga
     3421 ttttctggtg gtaaccaaca aaaggccatt attgctcgtg aagtggaccg tgatccagat
     3481 ttacttattg ttagccaacc aacacgtggt cttgacgtag gagctattga atatatccat
     3541 aagcgtttga ttaaagaacg tgataaaggt aaagctgtcc tcgttgttag ttttgaactt
     3601 gatgaaatat tgaatttatc tgatcgaatt gctgttattc atgatggtaa aattcaaggt
     3661 atcgtattgc cagaaaatac caataaacaa gaattgggta ttttaatggc aggtggttca
     3721 attcataagg aggaaggtca tgtctaaaaa tgcgcaaaaa attgctgtac cattgatttc
     3781 tgtcttgcta ggatttttac ttggcgctat tattatggtt atctttggtt atgatcctat
     3841 ctgggggtat gaaggtttat tccaaattgc ctttggtagt gttaaaaata ttggagaaat
     3901 attccgttca atggggccat tgattttaat agcgcttggc tttactgttg ctagccgtgc
     3961 aggcttcttt aacgttggtt tgtctggaca ggctttagct ggatggattt cagcaggttg
     4021 gtttgcactc ttaaatccag atatgcctcg tccattattg attcttatga cagctcttat
     4081 cggaatgatt gctggtggta ttgctggagc tattccaggg atacttcgtg cttatcttgg
     4141 tacaagtgaa gttatcgtta cgattatgat gaactacatc attttatatg tcggaaatgc
     4201 tattgttcaa cgtggttatc ctgagagtgt caaacaaagt attgattcaa ctattcaagt
     4261 tagtgataat gctagttatc aaacgcattg gttatcagcc ctaactaata actctcgtat
     4321 taatattggt attttctttg ccattattgc cattgcccta atctggttct tgcttaacaa
     4381 aactacttta ggttttgaga ttcgttcagt tggtctcaac ccacatgctt cagaatacgc
     4441 agggatgtca tcaaaacgta ctatcatttt atcaatgatt atctctggag ctcttgcagg
     4501 tcttggagga gtagttgaag gtctaggaac ctttgaaaat gtttttgtac aaggatcttc
     4561 tttagcagtt ggttttgacg gtatggcagt tagcttatta gcagcaaatt caccgcttgg
     4621 aatatttttc tcatcattcc tatttggtgt cttaaatatt ggtgctccag gaatgaatat
     4681 tgcaggcatt ccaccagaac ttgttaaagt agtaactgca tcaatcattt tctttgttgg
     4741 ttcacattac ctcattgaac gttacattat tcgccctaaa aaattagtga aaggtggtaa
     4801 ataagatgag tttagtaaca atttttgcct tattggtgtc atctatgctt atttatgcaa
     4861 caccactcat tttcacaagt attggaggaa ctttctcaga gcgctcaggc gttgttaatg
     4921 ttggtttaga aggtatcatg gtcatgggag cgttctctgg gattgttttc aacttagagt
     4981 ttgctgaaac atttggaaaa gcaacgcctt ggatagccgt tttagtcggt ggtattgttg
     5041 gtttgatttt ttctttgatt cacgctgtag caaccatcaa ctttcgagct gaccacattg
     5101 tcagtggtac agtgttgaac ttgttagcac cttcttttgc tgtcttcttg gttaaagcta
     5161 tgtacggtaa gggacaaaca gacaacattc aacaatcttt tgggaagttt gatttcccag
     5221 gtttatcaca aattcctgtg attggtgata ttttctttaa aaatactagc cttattggtt
     5281 acttcgccat tgctttttcg ttctttgctt ggtttttgct ttataaaacc aggtttggtc
     5341 tgcgtttacg atcagttggg gaacaccctc aggcagctga cacacttggt attaacgtct
     5401 atttgatgaa atactatggt gtgatgatct caggttttct tggtggaata ggtggagctg
     5461 tttatgctca gtcaatctcc gttaactttg ctgtaacaac tatcttaggt cctggattta
     5521 ttgctttggc agctatgatc tttggtaaat ggaatccagt tggcgccatg ctatctagtc
     5581 ttttctttgg cttatcgcaa agtttagccg ttattggtgc tcaattacca ttactagaaa
     5641 agattccaac ggtttactta cagattgcac cttatatggt aacgattatt attttagctg
     5701 ctttctttgg tcaagcagtt gcaccaaaag ctgatggaat caactatatt aaatctaaat
     5761 aacgttatat tagtagtaag cctcttagtg aggtttaaca gcactcctaa agcaagatag
     5821 ttaggtctag ctttaagggt gcttttttgg gacttaccgc aagcgtttgg attgccagtc
     5881 accatcacaa tagaagcaag aatagactaa aaagaactaa gtcagcggct tagttctttt
     5941 tgcttgtctt tcgtttttac tgatagctct taagttgttt tagccaacaa aggtattgat
     6001 ttctgtttca atgttagcaa ttttttcttg agcgatgtca agagtatcac ctattgtggc
     6061 aatatagaac ttgatttttg gttcagttcc tgaagggcga actgcaatcc atgaatcatc
     6121 agctaacgtg tacttcaaaa cattacttgg aggagttgtt aagttagata ttgttccatc
     6181 atttttagta gcggtttgtt tttggaaatc ttctaataat acaatatcag tattgttaaa
     6241 ttgttttgga ccattttcac ggaatttgtc cataatcttc ttaatttcag cagctccatc
     6301 cacacctgaa agtgttactg aaatcgtttt ttcagcaaaa tagccatatt ctttgtaaat
     6361 ttcatcaata ccatctgcaa gtgttaaacc acgcgaacgg taataagctg cgatctcagc
     6421 aaccaaaaga actgcttgaa tagcatcttt atcacggaca aatggtttaa taagatatcc
     6481 gaagctttct tcaaaaccaa acatataggt gtggttatgt ttttcttcaa actcttgaat
     6541 tttctcagcg atgaatttga atccagttaa aacgttaaac attgtagcgc cataactttc
     6601 ggcaattttt gttactaatt ctgttgatac gattgattta gcgagagcag tattttctgg
     6661 aagtgttcct gctgttttat gagcttccag gatgtatttc gcaataatcg ctccaatttg
     6721 atttccagaa aggtttttat aagaaccatc tggttggcga atttcaacgc ctaggcggtc
     6781 tgcatcagga tcagtagcta ctaagacatc tgcatcaact tctcgtccga gttcttcagc
     6841 aagagcaaag gcagcttggc tttcagggtt aggcgattta acagtagaaa agtctggatc
     6901 tgccttagct tgtgattcga ctacaacaac agattcaaaa ccggcttgag caagggcacg
     6961 gcgtgtcaac atctcaccag ttccgtggag aggagtatag acaattttca tgtcttttcc
     7021 aaagttattg attaaatctt gattgatatt aacatcttta acctctctaa gatattccat
     7081 atcaacatct tcaccgataa cttgaataag acctgatgat ttgctttcat ctaaatcagc
     7141 aagttcaact gcaaatggat tttcaatagc acggataaag tctgttagtg catcagcatc
     7201 agctggtggt agttgacctc cgtcttgtcc ataaacttta taaccattga aaggtgctgg
     7261 attgtggctt gcagtaacca taataccagc ataggcattt aaatggcgaa cagcaaaaga
     7321 gagttctggt gttggacgaa gagcttcaaa tacgtaagat ttaatcccat gctgtgccaa
     7381 gacctgtgca gactcaaaag caaattctgg agagaagtgg cgcgaatcat aagcgatggc
     7441 tacaccacgt tttttagctt cctctccctt agtttcgatt aattttgcta atccttcggt
     7501 tgcttgacgg acaacataaa tattaatacg atttgtacca gcaccaatat aaccacgcat
     7561 accagcagta ccaaattcta agtttgtgta aaaagcatct tcttttgttt tttcatccat
     7621 tgatagtaat tcttgacgaa gatagtcagg aagctgctca aaatctaacc atttttgaaa
     7681 attttctgtg taagtcattg aaatactcct tcaatattta taaacgcttt cattatatca
     7741 ttttgaagga aaaaaaacac taattatttg cagctgttta caaaactttt tccatatttt
     7801 caagtagcta atgtaagaaa gttaaaataa ccaagttttt aaaagagaaa aactcggtta
     7861 ttgtgaaagc taattacctt tttagtcttg atagtgttgg aataagtaca aaggtaataa
     7921 tagctgagat gatcatctca acaatagcgt ttgatgagat gatggctgtt agtagtgctt
     7981 taatatttcc atcaaaaact gtcgcgaaaa aaataaatat cccagataat acaaatatag
     8041 tatttgttag agaaccaatg ataccggata ctattaaacc aaactgatta ggaatcaatt
     8101 tatagcaata ataaggggta ataccaatca aaatacgtgg gataatagca attattaaag
     8161 aagcgaaagt tccatgatcg acaaaaggtg aaaagagata atttgtaggc agtagaatga
     8221 tggtatttgt aatgacactt ataatcccca ttaatcctcc taaaatagca cctatccttg
     8281 ggccatataa gacagaagca ataataactg ggatatggac aagcgttggt ttgattggaa
     8341 taggccaaat attaaagaca agactgctca caaaatgaat gactaacatg atggcaaaaa
     8401 agatagcaat acgagaaata tctgctgatt tacggtgatt cattttactc tccaaaaaat
     8461 ataatactta attctaaaaa ggtgatcaga aaaacgactg ttaaagtttc caaatcgtat
     8521 caatagtagc taagataaca tcaatatcag ccaaagcacc aggtccttta tctccacagg
     8581 ctaataagct agattttgga ggaatttctg taaaccctat tgtcgataag cgtttaatat
     8641 tttcttgggt aatagggttt tgatacattt tggtattcat ggctggggct attaattttg
     8701 gtgtggtagc tggtagggca agagcaacac ttgttacaat attatcagcg aatccatatg
     8761 ctagatgagc aatagtatta gcagaggcag gtgcaacaat aaataaatct gtgcgttttg
     8821 ctaattcgat atggttgata acttttgggt catgctcatc cataacatct aaatgaatag
     8881 cattttttga taacacttgc agggttaatg gtgtaataaa ttgtgtagca gcctgagtca
     8941 ttatgatgtg aacatcatag ccaattttag ttaattgact cgtaagatct gctgctttat
     9001 aggcagatat actaccagaa acagctaagg taatatgttt agtcatgttt ggtcatcctt
     9061 tcatataata agtcagcaat tgcctctttg gtatctgcag atgcaacttc gttattggat
     9121 atcaaaagag ccttgtgcat acctgtttgg atatcaacta aatcattggc gagaatataa
     9181 tcagcatggt ttttagctag acttgctctg gctactttaa ttaactcttc ttgaggcaca
     9241 ttaactagaa gtttaaagcc gactagttta atattaggat tccactgttt aacataagag
     9301 ataactttgg gtgttttttt caaaaaaaga acctgataat cagatgcaga tgagattttc
     9361 ggctcactgt tatgttcaca tagaaaacaa ttaaggttat cagcttgaga gacttgttcc
     9421 aaatcagtca tgtagactgg agtatagtca gaaacagcca tactatgaat tagaatgtca
     9481 tggtgaggaa cctgatcttt gagtgcagcc attaagtcgt taactgtttc cacttcaata
     9541 atccttaggc gcttatttgg caggggttta gtggctgttt tagtagtgac tagggtaacg
     9601 tcatgatgat attgtaaaaa tcgctctgtg attaattttc caagttgccc agttgagtga
     9661 ttagtgatac ctcgtactgc atctataggt tcagttgttc cacctgatgt tattattaat
     9721 ttcatagtca tattttatca aaaaaggagc tgatagtaaa cggaagataa tgacgccaga
     9781 cattgtcttg cgtttgtatt aatatggaat agtaatagat ttctagtaat taaaacgaag
     9841 ttctggaaag caagacacta tagtagctaa aatctaaatt ttaaggtaaa atagagcaag
     9901 cggtttcaag tgtgaaagga agaattgtga taactattag agatttagcg tcactgccga
     9961 tttttgttta cggtgataga aataaaaaag ttccaggagc tttgagtcct tttgtgtgga
    10021 cagaggtgtt tttaaaaact atcaatcaag aacctaatca actgatctta catatttggc
    10081 cgatgacaag aacagttata ttagggatgt tagatagaca gcttccctac tttgagctgg
    10141 ctaaaacaga gattggcaat aatggttatg ttcctgttac tcgtaatatt ggcggtttgg
    10201 ctgttgtcgc tgatgatggg attttgaatt tttcgcttgt tattccagac catttttcgg
    10261 aatctatcag tattagtaat gcttatttaa taatggttga tgtcattaga gagagttttt
    10321 cggattatta tcagcgtatt gaatatcacg aaattaaaaa ttcttattgc cctggtaatt
    10381 ttgatcttag tattgcaggc cgcaagtttg caggtatcgc ccagcgtcgt atcaaaaagg
    10441 gaattgttgt gtctatttac ttgagcgttt gtggggatca agcagcgcgt ggtcaactca
    10501 tcaaggattt ttatgaggcc ggaacacaag gagaagttac caaggttaat taccctcaaa
    10561 ttgatccaga atgcatggca actctttcag aactccttga gacgcctttt acagtggcag
    10621 aagtccttga acgtcttcga ttgaccttgc gacagcttgg cttttccctc acggaaaaaa
    10681 gtccagatca agccttactg actaattttg atgctgtcta tgaacggatg cagctagaag
    10741 tcgtcagaaa ggaaggcaaa tgaaagaaag atttagtcct ttatttgagc ctttgacttt
    10801 gccaaatggg agtcaattgg ataatcgctt tgttttgtct cctatggtga ccaactcatc
    10861 cacaaaagac ggttatgtca cccaagatga tgtatcctat gctttgagac gagcagcttc
    10921 tgcccctcta caaattacag gagcggccta tgtggatcct tatggtcaat tgtttgaata
    10981 tggttttagt gttacaaaag atgccgatat ttctggcttg aaagagctag ctcaagcgat
    11041 gaaggcaaag ggagctaaag ctgtcttaca actgacccac gctggccggt ttgctagcca
    11101 tgccttgact aaatatggct ttgtctatgg gcctagttac atgcagttga gatctcccca
    11161 acctcatgaa gtcaaacctt taacaggtca gcaaatagag gaattgattg cagcttacgc
    11221 tcaggccact cggcgggcaa ttcaagcagg ctttgatggc gtggaagtat cttcggcgca
    11281 gcgcttattg attcaaacgt tcttttcgac tttttcgaac aagaggacag atagttatgg
    11341 ttgtcaaaca ctttttaatc gctctaagtt aactttagct gttttgcaag ctgttcaaca
    11401 ggtgattaat caagaagctc ctgatgggtt tatttttgga tttcgtgcta caccagaaga
    11461 aactagggga aatgatattg gttatagtat tgacgagttt ttgcagttga tggattgggt
    11521 acttaatatt gcaaaacttg attatttagc gatagcaagt tggggccgtc atgtatttcg
    11581 aaatactgtt cgttctcctg gcccttatta tggaaggcga gttaatcagg tagttagaga
    11641 ttatttaaga aataagctac ctgttatggc tacaggggga atgaacacac ctgacaaagc
    11701 tattgaagca ctagctcatg ctgattttat tggagtatct acaccttttg tcgtggatcc
    11761 agaatttgct cataaaatca aagaagggtg tgaagagagt attcatttaa ggattcgacc
    11821 tgctgatttg aaaagtttag cgattcctca ggcttctttc aaagatattg tacctttgat
    11881 ggactacggg gaatcattgc ctaaagaatc aagaacttta tttcgtagtc ttactcacaa
    11941 ttataaggag attaaatgaa agtcagtatt cttgattatg gggtgattga taaagaaaaa
    12001 acaccccaag aagctttatt agaaacgcgc tgcttagcac aagtagccga caaacttggt
    12061 tttcatcgtt tttgggtagc agagcatcat aatatttatg cctttgcaat aagcagtcca
    12121 gagctgttga tgatgcattt agcagatcat accaaacaga ttcggatagg ttcaggaggt
    12181 attatgcctc tacattatag tagctttaag attgcagaat ggataatgac tttagaggcc
    12241 ttgcacccta atcggattga tttaggtatt ggcaactctt taggaactac gttagttcaa
    12301 agagctctat caagtatcca ttgcaaagac agctatagtc aggttgtcac agaattatac
    12361 cagtatctta atcctgatca tttgtcacca ctaccgattt ttgttaaccc taggggtaat
    12421 acttaccctc agatttggac gctaagcaat agtttggaga cagcagaact ggctggtcaa
    12481 ttaggtttag gctatacctt tggtattttc ccctatatac caaaagatcc tataacagaa
    12541 gctaaaagag tatcggcaca ttatagaaag gcttttaggc cttcaaagct attaaaaatc
    12601 cccaagttaa tattagcagt ttttattgtc ctttctgata ccgacgagga agcagaagct
    12661 ttggctaaac cgttggacat ttggatgctt ggtcagcaag attttaatga atttaagacc
    12721 taccctgatg ttgaggaggc tagaaactat cacttaacag agaaacaaag agaagccatt
    12781 gcagcaaatc gtagtcgcat ggttattgga tcacctcaca cagtcaagaa acaattggat
    12841 aggctgatag aagcttgtca agctgacgaa ttattagcca tccccttagt tcctgaattt
    12901 gccaatagac aacgaacctt agagctgcta gcagacttat acgtgaccat ttaaccttac
    12961 caaaaacaat tccctaaact gaaaggaaag aaacaatgaa aaaaattgca aattacttat
    13021 taattgagaa aacagatgac cgctatacca ttagcatgac accagaatta caagacgata
    13081 ttggtactat tggttacgct gaatttacgg ataacgacca ccttgccgtt gatgacatta
    13141 tcttaaatct agaggcttct aagaccgtca tgtctgtctt gtcaccattg gctggggcag
    13201 ttgttgagcg caatgaggca gccacactaa caccaaccct tttgaattca gaaaaagctg
    13261 aggaaaattg gattgttgtg ctaacagatg ttgatcaagc agcttttgat gccctagaag
    13321 atgcctagtt catttgattt attaggggaa atgattggtc tgttgcaaac agagcagctt
    13381 actagttctt gggcctgtcc cttgcctaat gctttaacaa agcgacaaga cttgtggcgg
    13441 gcgctcatta accagcgccc tgctttgccc ttatcaaaag attacttgaa tctggaagat
    13501 gcttatctag atgattggcg tgcttctttt gttcctgtat cagtcaagga ttgccaaaaa
    13561 actaattata ccagtctttt tctttaccat ggggatatta ggtaccttgc tgtggatgct
    13621 attgtcaacg ctgctaatag tgagttattg ggatgtttta ttcctaacca tggctgtatt
    13681 gataatgcga ttcatacgtt tgccggaagt cgtttacgct tagcttgtca ggcaatcatg
    13741 actgaacaag gccgtaagga agccattgga caagcaaagc ttaccagtgc ttatcatcta
    13801 ccagcctctt atattattca taccgtaggg ccaagaatta ccaaaggaca ccacgtttct
    13861 ccgattcgag ctgatttgtt agcacgttgt tatcgttcta gtcttgattt ggcggttaaa
    13921 gcaggattga ctagcctagc tttttgctct atttcaacag gagaatttgg ctttcctaaa
    13981 aaagaagccg ctcagattgc tattaagaca gttttaaaat ggcaagcaga acatccagaa
    14041 tctaagacct taactatcat ttttaatacc tttacatcag aagacaaggc cttatacgac
    14101 acttatttgc aaaaggagaa taactgtgag taattggaca acctatcctc agaaaaacct
    14161 cacccaggca gaacaactgg cccaattaat caaagaagca gatgcacttg tagttggtat
    14221 tggagctggc atgtctgcag ctgatggttt tacttatatc gggccgcgtt ttgagacggc
    14281 attcccagat tttattgcta agtatcagtt tttggatatg ctgcaagcta gtctttttga
    14341 ttttgaagat tggcaagaat attgggcttt tcaaagtcgc tttgtcgctt tgaattatct
    14401 tgatcagccc gtaggccagt cttatcttga tttaaaagag attttggaaa ccaaagacta
    14461 tcatattatt actactaatg ctgataatgc tttttgggtg gctggttatg atcctcataa
    14521 catttttcac attcaagggg aatatggttt gtggcaatgt agccagcact gccatcagca
    14581 aacttataag gacgatacgg tcatccgtca aatgatcgca gagcaaaaaa acatgaaagt
    14641 cccaggtcaa ctgattcctc attgccctga atgtgaggcg ccttttgaaa tcaataagcg
    14701 taatgaagaa aaaggaatgg ttgaagatgc tgactttcat gcccaaaaag cacgttatga
    14761 ggctttttta tcagaacata aagagggtaa agtgctttat ctagaaattg gtgtgggaca
    14821 tacgacgcct cagtttatca aacacccctt ttggaaacgt gtctcagaaa accctaatgc
    14881 cttattcgtt accttgaatc acaaacatta ccgcatccct ctgtcaataa ggcgacaaag
    14941 cctagaactg acagaacaca ttgcacaatt gattagcgcc acaaaaacca tttaccaaaa
    15001 atcgtaacca aagccaatag acggttaatt aagagatata gaaggagact aacatgtacc
    15061 ttattgaacc gattcgtaac ggtaaacgta tcaccgatgg agctgttgct ctagccatgc
    15121 aagtttatgt tcaagaaaat ctatttttag atgatgatat tttatttccc tactattgtg
    15181 atcccaaagt tgaaattgga aaattccaga atgcggttgt agaaactaat caagagtatt
    15241 taaaagagca tcatatccct gttgttcgcc gagatactgg cggtggtgcc gtttatgtag
    15301 attctggtgc tgttaatatt tgctacttga ttaatgataa tggtattttt ggtgatttta
    15361 aaagtaccta tcagcccgct attgaagccc ttcatcattt gggagcaaca ggggttgaaa
    15421 tgtctggacg gaatgacttg gttattgatg ggaaaaaggt atctggcgct gcgatgacta
    15481 ttgctaatgg ccgtgtttat gggggatatt cccttttgct agatgtggac tttgaagcca
    15541 tggaaaaagc attaaaacct aatcgcaaaa aaattgaatc aaaaggcatc aggtcagttc
    15601 gtagtcgcgt tggaaacatc agagaacatt tagcaccaca ataccaaggt attacgattg
    15661 aagagttcaa agatttaatg gtttgccaat tgttacagat tgagacgatt agccaagcta
    15721 agcgttatga tttaacggaa aaagactggc aacaaattga tgctttaaca gagcgtaaat
    15781 atcacaattg ggaatggaat tatggcaacg cgcctcaata ccgttaccat agagatggcc
    15841 gttttaccgg tggaacagta gatattcatt tagatattaa aaaaggctat attgcagctt
    15901 gtcgcattta cggagatttt tttggcaagg cagacattgc agaacttgaa ggtcacttga
    15961 taggaacacg aatggaaaaa gaggatgtct tagctaccct caatgcaatt gatttagccc
    16021 cttatttagg tgcgattaca gcagaagaat taggagattt gatattttct tgataaaaaa
    16081 gatacctcag accggtaaag tgcgattaaa aacgaacaat aattgtttta attcaaataa
    16141 atgtacgtat gatatgaatt gtgatataat gacgtcatta cgtaaaattg aggagtcatc
    16201 atgaaatcag atatcgaaat tgcccaaagt gtcgccctac aaccaatcac tgacattgtt
    16261 aaaaaagttg gaatagacgg cgatgacatt gagctttatg gcaaatataa ggctaaattg
    16321 tcttttgaga aaatgaaagc tgtcgaagct aatgagcctg gaaaattaat cttggtaact
    16381 gctattaacc ctactccagc gggagaagga aaatcaacca tgtctattgg actggcagat
    16441 gccttaaatc aaatgggaaa aaaaaccatg cttgccttac gtgaaccctc ccttggtcct
    16501 gttatgggga ttaaaggcgg ggcagcaggt ggtggttatg cccaagtatt gcctatggaa
    16561 gacattaatc ttcactttac tggtgacatg catgccatta caactgctaa taatgcatta
    16621 tctgctttga ttgacaatca tttgcagcaa ggaaacgatt taggaattga tcctcgccgc
    16681 attatttgga agcgcgtgtt ggaccttaat gatcgcgctc ttcgtcaggt aattgttggc
    16741 ttaggtagtc cagtgaatgg tgtgccacgc gaagatggat ttgatatcac agtagcttct
    16801 gaaatcatgg caattttatg tttggcaact gacttaaaag atctaaagaa acgtcttgct
    16861 gatatcgtcg tggcttatac ctatgatcgt aagcctgtct atgtgcgtga cttgaaggtt
    16921 gaaggagcat tgacattaat tctaaaagat gctatcaagc caaacttggt gcagactatt
    16981 tatggaacac cagctcttat tcatgggggg ccatttgcta atattgctca tggttgtaat
    17041 tctgtccttg ctacctcaac agctttgcga ttagcagatt acactgttac cgaagcagga
    17101 ttcggggccg acctaggagc tgaaaaattc ctaaatatca aagtacctaa tcttcctaaa
    17161 gcaccagatg ctatcgtcat tgttgcaacc ttgcgcgccc ttaaaatgca tggaggtgtc
    17221 gccaaatcag atttagccgc tgaaaattgt gaggctgttc gcttaggatt tgccaactta
    17281 aaacgtcacg tggaaaacat gcgccaattc aaagttccag ttgtggtggc tatcaatgaa
    17341 tttgtagcag ataccgaggc tgaaattgct accttaaaag cgctttgtga agagattaag
    17401 gttccagtag aattagccag tgtttgggca aatggagctg agggaggctt agcattagct
    17461 aaaacagtgg ttcgtgtgat tgatcaagaa gctgctgact acaaacgtct ctattcagat
    17521 gaggatactt tggaagaaaa agtgatcaat atcgttaccc agatttatgg tggcaaagct
    17581 gttcaatttg gccccaaggc caaaacacaa ttgaagcagt tcgcagaatt cggttgggat
    17641 aaattgcctg tttgtatggc taagacccaa tacagttttt cagataaccc atccttgctt
    17701 ggagcgccaa ccgattttga tattaccatt cgtgaatttg ttcctaaaac aggggcaggt
    17761 tttattgttg gtttaacagg tgatgtgatg accatgcctg gtttgcctaa agttccagct
    17821 gccatggcaa tggacgtagc agaaaatggc acagccctcg gtctctttta ggaaaaattg
    17881 ctcgaataat tcgagcaagc gctcctttct tcccgcttta ctgtgatata ataaggtaaa
    17941 tgagattttg gagggatgat tatcaaaaag aaagcaaaag taaaatattt gctccataaa
    18001 gggaaacatg gatttttaag aggaattttt agtagaacaa caatcattgt attattaatt
    18061 attttgcaat tagttttctt atttcaatct tatgcctgga tggagcagta ccgtgtttgg
    18121 at
//
LOCUS       DAUGFP010000030        18005 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.030, whole genome shotgun sequence.
ACCESSION   DAUGFP010000030 DAUGFP010000000
VERSION     DAUGFP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18005
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.030"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            91..1509
                     /locus_tag="VH663_001116"
     CDS             91..1509
                     /locus_tag="VH663_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ8174324.1"
                     /translation="MDKKIQRFSCTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMI
                     VVKPEDQPRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATE
                     TITTNSRVLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVA
                     FSDEHDIWWLETIGGHHWIARRVPDDAYVTNPNQFGIDHFEFNNPEDYLCSADLKDFI
                     DTYHLDLTYSHEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPRSFALAWC
                     QKPYRKITVEDVKYVLSSHYQDTGYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRP
                     NKPQEIAAIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAAL
                     ADPHYNHHETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQ
                     TLLNKILFDASNLMTNRFSLSD"
     gene            1661..3208
                     /locus_tag="VH663_001117"
     CDS             1661..3208
                     /locus_tag="VH663_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEQ8174325.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPIYEF
                     TKGIVGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDTDAFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGAGHDHHHEDADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTKKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGERKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            3359..4081
                     /locus_tag="VH663_001118"
     CDS             3359..4081
                     /locus_tag="VH663_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8174326.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMIENDKLLSERELSDLYGVSRI
                     TIRLALKELELRDLIYKKQGKGTYVSGIKEPVTDLSSTYSFTEQMKKVGKTPKTEILS
                     FEQYQVTPYLSGLLEFDPDTEVFELERLRIADDMPLMFERSYIPAQLFQGLSIEHLKR
                     KPMYDLFAEDYQETIRLAEEEFYASIALDYEAGLLGIKKGDPVLHIIRKTYNDNNILI
                     ELTFSIARADQFRYRVQHHPNG"
     gene            4100..5299
                     /locus_tag="VH663_001119"
     CDS             4100..5299
                     /locus_tag="VH663_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEQ8174327.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFDETRDSLDS
                     FLKRVCNSANRNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQRYLFESVASTDLVAAP
                     DYYLVEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKILALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQYNKA"
     gene            complement(5367..5627)
                     /locus_tag="VH663_001120"
     CDS             complement(5367..5627)
                     /locus_tag="VH663_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEQ8174328.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(5742..6683)
                     /locus_tag="VH663_001121"
     CDS             complement(5742..6683)
                     /locus_tag="VH663_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEQ8174329.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLAWMSQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            7077..7526
                     /locus_tag="VH663_001122"
     CDS             7077..7526
                     /locus_tag="VH663_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEQ8174330.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSEQFALTGAIKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            7736..8020
                     /locus_tag="VH663_001123"
     CDS             7736..8020
                     /locus_tag="VH663_001123"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEQ8174331.1"
                     /translation="MRLEEIRQEINSIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QVRENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRKYQTQHLTGGDTND"
     gene            8013..9275
                     /locus_tag="VH663_001124"
     CDS             8013..9275
                     /locus_tag="VH663_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEQ8174332.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGVAVGVIDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILLSTWVTHLFGASAGREGVAVQIGATISHYCRRFVTSQDAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFACLLGWFKPYLSQKLPNPYIRIAFIGVLLSICLMIGHV
                     GRYSGLGTHLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGII
                     LAPYLGLPVLLVAALGYVTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            9390..9737
                     /gene="rplS"
                     /locus_tag="VH663_001125"
     CDS             9390..9737
                     /gene="rplS"
                     /locus_tag="VH663_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEQ8174333.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            9985..10056
                     /locus_tag="VH663_001126"
     tRNA            9985..10056
                     /locus_tag="VH663_001126"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10018..10020,aa:Arg,seq:cct)
     gene            10748..11317
                     /locus_tag="VH663_001127"
     CDS             10748..11317
                     /locus_tag="VH663_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEQ8174334.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVATFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            11318..13270
                     /gene="gyrB"
                     /locus_tag="VH663_001128"
     CDS             11318..13270
                     /gene="gyrB"
                     /locus_tag="VH663_001128"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEQ8174335.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEIIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRDFIEENAVYSTLDI"
     gene            13638..15362
                     /gene="ezrA"
                     /locus_tag="VH663_001129"
     CDS             13638..15362
                     /gene="ezrA"
                     /locus_tag="VH663_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEQ8174336.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(15494..15952)
                     /locus_tag="VH663_001130"
     CDS             complement(15494..15952)
                     /locus_tag="VH663_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEQ8174337.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQTPEKTKSFWKRFLKK"
     gene            16185..17492
                     /gene="eno"
                     /locus_tag="VH663_001131"
     CDS             16185..17492
                     /gene="eno"
                     /locus_tag="VH663_001131"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEQ8174338.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
BASE COUNT         5592 a         3165 c         3755 g         5493 t
ORIGIN      
        1 aagataagga ggtgctgctg gaattatgaa aatttttgta tccagttcaa tggatatttg
       61 atataatcgt cttaaaaagg aggtcgtgaa atggataaaa aaatacagcg tttttcatgt
      121 acaactatac tcgtcggtaa aaaggcatct tatgatggct caaccatggt tgctcgaaca
      181 gaagattctc aaaatggtga tttcacgcct aaaaaaatga ttgtggtgaa accagaagat
      241 caaccgagac attaccgttc ggtgcaatct tcctttgaaa tggacttacc ggacaatccc
      301 atgacttaca cgtctgttcc cgatgcgctt ggtaaagatg gtatctgggc agaagcaggc
      361 gtgaacgagg ctaatgtggc catgtcagca actgaaacca tcactaccaa ttctcgtgtt
      421 ttaggggcag atcctctagt agcttctggt attggtgagg aggacatggt gaccttagtg
      481 ctcccttata tccgttctgc gcgtgaaggg gttttgcgcc ttggtgccat tttagaagac
      541 tatggcactt acgagtcaaa tggtgtggct ttttcagatg aacatgacat ttggtggctg
      601 gaaacgattg gtggtcatca ctggattgca agacgggtgc cagatgatgc ttatgtcacc
      661 aatcccaatc aatttggcat tgatcatttt gaatttaaca atcctgaaga ttacctttgc
      721 tcagctgatt taaaggactt tattgacacg tatcacttag atttaaccta tagccatgag
      781 cattttaatc cgcgctatgc ttttggtagt caacgtgaca aagaccgtca ctataatacg
      841 ccacgtgctt ggatcatgca aaaattctta aacccagaaa ttgtgcaaga cccgcgcagc
      901 tttgcccttg cttggtgtca aaaaccttac cgtaaaataa cagtagaaga tgtcaaatat
      961 gtattgagta gtcactatca ggacacgggt tatgaccctt acggttcaga aggaactcct
     1021 gttagtaaga aagtctttcg ccctattggc attaaccgta ccagccaaac cgctatctta
     1081 cacattcgtc ctaataaacc tcaagaaatt gccgctattc aatggatggc ttatgggtca
     1141 atgccattta ataccatggt gccttttttc acacaggtga agactattcc agattatttt
     1201 gccaacactt acgaaaatgt gtctaccgac aatttctatt ggaccaaccg tctgattgcg
     1261 gctttggcag atcctcacta caatcatcat gaaactgatt tggacaatta cttggaagaa
     1321 accatggcaa aaggtcatgc catgttacat gctgtggaag cacagttatt ggctggtgaa
     1381 acagtcgatt tggaagagga aaatcaaaaa atgagtgact atgtgcaagg agaaacgcag
     1441 acgctgttga acaagattct ctttgacgct agcaatctaa tgaccaatcg tttcagtttg
     1501 agcgattaat agtgaagcta aggctgagca actgcttagc cttttttacg ataacttttt
     1561 aggaaaagta atcgcaaaaa gacaaaaact tggaaaaaaa atcaaagtat gctatactta
     1621 actggtcaat ttttaaccag ttaataacaa aggaggaatc atgaaaaaga aaattctttt
     1681 aatgatgagt ttaatcagtg tcttttttgc ttggcaactt actcaggcaa aacaagtctt
     1741 agcagagggt aaagtgaagg tggtgacaac tttctatcct atttatgaat ttacaaaagg
     1801 gattgttggt aatgatggcg atgtttccat gcttatgaaa gcaggaacgg aacctcatga
     1861 ttttgagcct tctacaaaag acattaaaaa aatccaagat acagatgcat ttgtttatat
     1921 ggatgacaat atggaaactt gggtttctga tgtgaaaaaa tcattgacat ctaaaaaagt
     1981 gaccatcgtc aagggaactg gtaacatgct cttggtagca ggagctggac atgaccatca
     2041 ccatgaggat gctgacaaaa agcatgagca taataaacat agtgaagaag gacacaacca
     2101 tgcttttgac ccacacgtgt ggttgtcacc ataccgtagc attacggtcg ttgaaaatat
     2161 tcgcgacagt ctttcaaaag cttacccaga aaaagcagag aacttcaaag ccaatgccgc
     2221 tacttatatt gaaaaattaa aagagcttga caaagactat acggcagcac tttcagatgc
     2281 taagcaaaag agctttgtga cacaacacgc agcttttggt tatatggcac ttgactatgg
     2341 cttgaaccaa atttctatta atggtgtcac accagatgca gaaccatcag caaaacgtat
     2401 tgctactttg tcaaaatacg ttaaaaaata tggcatcaaa tacatttatt ttgaggaaaa
     2461 tgcgtcaagt aaagtcgcaa aaaccctagc taaagaagca ggagttaaag cggctgtgct
     2521 tagtccgctt gaaggtttga ctaaaaaaga gatgaaagct ggccaagatt actttacggt
     2581 catgcgtaaa aaccttgaaa ccttacgctt aaccactgat gtggctggta aagaaattct
     2641 tccagaaaaa gatacgacaa agacagttta caatggttat ttcaaagaca aagaagtcaa
     2701 agatcgtcaa ttatctgact ggtcaggtag ctggcaatct gtttatccct atctacaaga
     2761 tggtacttta gaccaagttt gggactacaa ggctaaaaaa tccaaaggta aaatgacagc
     2821 agccgagtac aaagattact acactactgg ttataaaact gacgtggaac aaatcaaaat
     2881 caatggtaag aaaaagacca tgacctttgt tcgtaatggt gaaaggaaaa ccttcactta
     2941 cacatacgcc ggcaaagaaa tcttgaccta tccaaaagga aatcgcggag ttcgtttcat
     3001 gtttgaagct aaagaaccaa atgctggcga attcaaatac gttcaattca gtgaccatgc
     3061 cattgctcct gaaaaagcag agcatttcca cctgtactgg ggtggtgaca gccaagaaaa
     3121 attacataaa gagttagaac attggccaac ttactacggt tcagacttat ctggtcgtga
     3181 aattgcccaa gaaatcaatg ctcattaatc ttaactcact aagcaaatct tcttttaagt
     3241 aaggaggttt gctttttctt tgtggagtaa aaaaattaat aattctcaac attaatttga
     3301 cggacactta tgattataat atactttaag tggttataac aagttagagg tgaataagat
     3361 gccaaaagaa caaccactct atctacagat tgttgatgac ttggaagtga aaatcaaaaa
     3421 aagcatgata gaaaacgata agttgttgtc agaacgagaa ttaagtgacc tttacggtgt
     3481 tagtcgtatc acgattagac tggctcttaa ggaattggaa ttacgagact taatttataa
     3541 aaagcagggc aaggggactt atgtttcagg aatcaaagaa cctgtcactg atttatcaag
     3601 tacttacagc tttacagagc aaatgaaaaa agtggggaaa actcctaaaa cagagattct
     3661 ttcttttgaa cagtatcagg taacacctta cttgtctggt ctcttagagt ttgatcccga
     3721 tacagaagtt tttgaattag aacgcctaag gattgcagat gatatgccct tgatgtttga
     3781 acgatcctat attcctgctc agctttttca agggttaagc attgagcact tgaagcgtaa
     3841 gccaatgtac gatttatttg cagaggatta ccaagaaact attcgcctag cagaagaaga
     3901 gttttatgct agcattgcct tggactatga agcaggtctc ttaggcatta aaaagggaga
     3961 tcctgtctta catattattc gtaaaactta taatgataac aatatcttaa ttgaattgac
     4021 tttcagcatt gcaagggcgg atcagtttag gtaccgagta caacatcacc ctaatggtta
     4081 aggaattgga ggttaggaca tgtttacaaa aacgcaggaa gctttggagg ctcttggtgc
     4141 agctatcact accaaggaaa ttaaacaaga accccgttta tggcaagaaa ccatgacgtt
     4201 ttttgacgaa acaagagatt ctctggattc ttttttaaag agagtttgta acagcgctaa
     4261 cagaaataac gttcatgtca tctttacagg tgcgggtaca tctgagtaca tcggtaatac
     4321 catttgccct tacttgaaga aggtggggaa tcggcagcgc tatctctttg agagcgttgc
     4381 ttcaaccgat ttggttgcgg cgccagatta ttatcttgtt gaggaagaga cggtactatt
     4441 agtctccttt gctaggagtg gtaatagtcc agaaagtgta gcggctgtta acctggtcaa
     4501 tcaactagtg ccaaatagtt atcatttaac cattacctgc gccaaagatg gagaactggc
     4561 gaaaaaagca cagcaagatg agcgttctta cctttatttg atgcctgaag acgctaatga
     4621 tgctggtttt gccatgactg gtagttttac ctgtatgatg ctagcggctc ttttgatttt
     4681 tgatgatgct aacagtatag ctcagaaaga agcttatgtg actgatatga ttcttgctgg
     4741 gcaagctgtt attgaccaag aaagacggct acaagagcta gctgatttag gatttgaacg
     4801 tttagtttat ctaggttctg ctggtttagc caagttaacc caagaagctc aattaaaaat
     4861 gttagaattg acagctggac aagttgcgac cctctacgag tcctctatgg gctttaggca
     4921 tggtccgaaa tcttttatta atgaccgtac cttagtcatt ggatttgtca ataatgatgc
     4981 ttacgtcaga cagtacgatt tggacatgtt agaggaaatc caagcagatg gcattgccct
     5041 taaaatcttg gctctattgc aaggagggga tatcaacttt tcagaggacc aatttcgact
     5101 agacacccgt cacttattgc cagaagctta cttagctttt cccatgattt tagtcgcgca
     5161 gaccttggcc ctcttaacgg ccgttacatt aggaaattcc cctgacaccc catctgcgac
     5221 aggaacggtt aatcgtgtgg tcaaaggagt aacgattcac ccttacccga ctagtagtcg
     5281 tcaatacaac aaagcttaaa gcttttgtgc tagtaggcca aaagaaaaac cctcctgata
     5341 caggaaggtt ttttgtattg tcttgcttat tttgctgcgt ttgcgtcttt gagaccgtat
     5401 tttttgttga agcggtccac acgtccatct gcttgagtga acttttgacg tcctgtgtag
     5461 aatgggtgtg aatctgatga aatttccaca cggataagtg ggtaagtttc accttcaaac
     5521 tcaacagttt ctttagaagc ttttgttgag ccgctaagga actggtaacc tgtagttgta
     5581 tcaaggaaaa caactggacg ataatctgga tgaatgtctt ttctcatctt aaaaaattcc
     5641 tttctgccat gacacttgat gcatcatagt tattaactat tctagtgtaa caaaacttga
     5701 atgacttgac aaggcttttc tttccttatt ttttaaaaat cctaaatctc ttggcaaaca
     5761 gcgatgagtt ctcggaaaat agcttgattt tcttctaaat tagctgagtt ggctccgcta
     5821 gcaaggggat gcccccctcc accgtgacgc ttagcaatgc cattaataat cttttcttta
     5881 ctgcgcatac gcacacggta gttgccgtca gttaactcaa caaaaatagc ccaagcttga
     5941 acgttatcaa ttttaccagg agcacagaca atagcagagc tttctgctag ggtcacgtca
     6001 aaatgtttta aggtttcttg gctgacaagg acataagcag ccccactctc atcaattgtt
     6061 aaatgctcaa agacgtagct ttgcagcttt gctattttca aaggaaacga atccatttgc
     6121 cttgaaatcg cagcaaagtc gaactcgaaa tgtctgagtt ggctagcaat ggaaagggtt
     6181 ttactagtgg ttgaggcata aagaaatcgc cctgtatcac caacgatacc agtgtataag
     6241 agcttagcag ctttgtcaga gagagtaaga ttctgactaa aggcaaagtc tgcaatgatt
     6301 tcacttgcgc tagaagcgct tgtgtccaca taatagaagt caccatacac atcatcgttg
     6361 ggatggtgat caatcttaat taagcactgc cccagtgtgt agcgctcatc atcaatcctt
     6421 ggtcgattcg ctgtatctgt aatgatgacc aaagcctctt tgtagtcttt gtcagtcacc
     6481 tgatccattt ggctcatcca agctaaacta ggctcatcaa aaccagtcat taaaaccttt
     6541 ttgtctggga aattttgtgc aataatttct ttcaagccgg cctgactacc aagagcatca
     6601 gggtcaggat tttgatggcg atggataata atagtttggt gagcttttat tttatctaaa
     6661 attgtttcaa aagttgttat catgctttaa tctttctttt tcaaaaagag gaaaataacc
     6721 gtttaacata gtcttcaaaa aacggtttgt gtcataagga ggtgtgcgtt atgttcagtg
     6781 gctaatgcta gaatttttta agattgtcaa agaccataaa ttgacatttt atctgaaaat
     6841 catatcctat tttattatag cataaaagta agctctagat aatggtaaat taatctagga
     6901 agaaaagagg ttagttatca gctatgatac tatcaaagct agtcttgcaa atgcaagtga
     6961 aaaaatagtt tttaagtgtt ttaagtaatc atatcaacct tctcgttaaa gaattagtgc
     7021 ttttcctaaa aaagtgtgct aaaataaaca tagtttagta attttgggag gaatttatgg
     7081 cattagcaaa aatcgtttat gccagcatga cgggaaatac tgaagaaatc gctgatatcg
     7141 ttgcaaataa attgcaagaa ttgggccatg atgttgacat tgatgagtgc acaacagttg
     7201 acgcttcaga gtttgagaat gctgatatcg ctgtcgttgc gacttatact tatggtgatg
     7261 gtgacttacc agatgaaatc gttgattttt acgaagattt acaagattta gatttagaag
     7321 gtaaaatcta tggcgttgtc ggctctggtg acacttttta tgactatttt tgcaaatctg
     7381 ttgatgactt ttcagagcaa tttgcgctta ctggtgccat caaaggtgca gagccagtca
     7441 aagtagattt ggcagcagaa gatgaagaca tcgatcgttt agaggccttt gcagagcaac
     7501 tctcacaggc tgttaataat ctataaaact ataactctca caggctgtta ataatctata
     7561 aaactattta gtaaaaatgt caggggcttc cttggcattt ttagcttttt aacctaaaat
     7621 aaagggaatg tcacttcagg gtaggaattg aaaaaacaat cagcttcacc ttgcttctga
     7681 tcctcttatt aactataata aaaagataat ctaatacatc tagaaaagga aagttatgcg
     7741 tttagaagag atacgacaag aaatcaatag tattgatcac cacttggttg ccttattaga
     7801 aaagcggatg gcacttgtag agcaagtaac agcttataaa ctagccaatc acttgcctgt
     7861 cttagatcag gtgcgggaaa atcagatcct agatagggtt tcttatctcg ttaaagacca
     7921 agcatttgag ccagctatcc atgagacgtt taaaaccatt atgtctcttt cacggaagta
     7981 tcaaacgcaa cacttaacag gtggtgacac taatgattaa atcaattgca acactgccta
     8041 aaccctcctt aaaagaagcg ttaggactaa taggcttggc gcttcccata ggggtcgcgg
     8101 ttggagtgat tgatgtgatt tttggtaaag ggcttttgtt tctttccgag tatcgcgatc
     8161 accacctttt cttgcttctt ccttttcttg ccttagcagg ccttgttatc gtttttcttt
     8221 atgataagtt aggcaaagag gttcgtcaag ggatgggatt ggtttttcag gtagggcatg
     8281 gtcaaaaaga tcagattcct cctattctca ttcctcttat tttgttatcg acttgggtaa
     8341 cccacttatt tggagcgagt gcgggtcgag aaggggtagc cgttcaaata ggagctacca
     8401 tctctcatta ttgtcggaga tttgtgacta gtcaagatgc cgcacgacac cttttgatta
     8461 tggggatggc agccggtttt gcaggacttt ttcaaacgcc aattgctgct gtcgtatttg
     8521 cccttgaggt tttgttggta ggaacgcttc gctactgcgc tttattgcct tccttagttg
     8581 ctgcttgcgt ggcttcttgg acctctcatt ctttgggatt agagaaattt acgattgttc
     8641 ttgaagaaac attgaccata actcccttaa ccttagttaa attaattggg ttaggtctta
     8701 tttttggtct ggtgggaaat agttttgcct gtttacttgg ctggtttaaa ccttacctga
     8761 gtcaaaagct accaaatcct tatatcagga ttgctttcat tggtgttttg ttatctatct
     8821 gtttgatgat tggtcacgtt ggaagatata gtggtctagg gactcatttg attgcggcag
     8881 ctttttctgg ccaaactatc ctaacttacg actggctttt aaaaatgatc gtgacggtga
     8941 ttagcttatc tgcaggcttt caaggtggtg aagtgacacc tctatttgct attggagctt
     9001 ctttaggcat cattttagcc ccttacttag gtcttcctgt actgttagtt gctgcacttg
     9061 gttatgtcac ggtatttgga agtgcaacaa ataccttttg ggcccctatc tttattggga
     9121 tagaggtctt tggtccagag aatgccctag catattttgt cacctcagct gcagcctata
     9181 tggttagtca cagacattcc atttacagtt atcaaaaagt agtcatgatc aatttaaaag
     9241 acatgacggt aaatcagatg gatttaaaaa aatagcttta caataaagaa gctttttgtt
     9301 agaataaatc tcgtgtgtaa tggacacacg aaaattacgg ctaatccgct aagacaagta
     9361 cttaagatta gtaagatagg agaataaaaa tgaatccatt aatccaaagt ttgacagaag
     9421 gtcaacttcg ctctgatatc cctaacttcc gtcctggtga cactgttcgc gttcacgcaa
     9481 aagttgtcga aggaactcgc gaacgtatcc agatctttga aggtgttgtt atttcacgta
     9541 aaggtcaagg gatctcagaa atgtacactg ttcgtaaaat ctcaggtggt atcggggtag
     9601 agcgtacatt cccaatccac acaccacgtg ttgacaaaat cgaagttatc cgccacggta
     9661 aagtccgtcg tgcgaaactt tactacttgc gtgcattaca aggtaaagct gctcgtatca
     9721 aagaaattcg tcgttagttt gaaccgaaac gtaacgtaat agaataaaaa agactagagt
     9781 ctctagtctt tttatgtcgt cgtgacaatg tgacaattgg tgattaacgc tatgctcgta
     9841 ggacactttg aaagttcctt agcagttata attagtatat tggaataaca aaatgaagca
     9901 aaataaactt tcagttatct gatatttcat cttgcattac cttattttct aggttaaaat
     9961 agaagacaag gtgaatataa gatagacccc ttagttcaat ggatataaca actccctcct
    10021 aaggagtagt tgctggttcg attccggcag gggtcattat tgtcactgaa aaagccttta
    10081 catcaaaggc ttttttgtat tactcaatta atgttaggag cacaaaagag gcagttcgat
    10141 tattaaccaa ttcctcccta tttacagatg tatggctata aatggacaat cttaagttag
    10201 ggtctgagtg actaaacctg tccctaacca cttttggtgg taatcctttt tcagctaaaa
    10261 gcgaaatgtg cgagtgtttg aataagtgag tatggtaatc accataaatt tttaggcgct
    10321 tattgatgtg tgcatttaaa attggtaaac cttttgaata tggaaagctg ttagcactct
    10381 gcttgtgatt aatctatcgt atgttaaaag tgacatttaa attaaatcat tttttattat
    10441 ttcaaaagga tttatcatat tggaaaatag cggtgcttct attggcattt tactattgac
    10501 aggaatttcc ctgccttttg tctcctaaag aagatcagct ttgatttcta acttgattag
    10561 agtgggcttg gtactgttta tgaattatca gtgcatactt tctggagatc aaaaaggaaa
    10621 ggagcagcta aagaggtgta tgtgttacca ttagaggaat agaacatcgc aagactcgtt
    10681 tattcagtca ttacgatacc tgttttattt gggaaataac tataatatta agtgaatttg
    10741 tgatataatg aaaggtatga attatcaaga ttatatatgg gatttgggag gtaccttact
    10801 cgataattac gagctttcaa cccaagcttt tgtccaaacg ctagcttttt tcagccttcc
    10861 aggagatcac gatgctgttt accaaaaatt aaaagaatca accgctatcg ctgtggcaac
    10921 gtttgctcct aatgagccag aatttttgca tgtgtatagg ctaagagaag cagacaaatt
    10981 ggcacaaccc atttggtgtt tgggagctaa agaaattttg gaaaagatag cgacttcagg
    11041 ttcgcgaaat tttttaattt ctcatcgaga ttgtcaggtg aaccaacttt tagagcaagc
    11101 tgggttgtta atctatttta cagaagtcgt gacagcttca aatggctttg ctcggaaacc
    11161 aaatcctgaa agtttgtttt atttaaaaga aaagtatgat attaatagtg gattagtaat
    11221 tggagatcgg ctaattgata aacaagcagg acaagcagcg ggttttaata ccttgcttgt
    11281 tgacggtaga aaaaacctat tggagatagt aacgtagatg attgaagaaa ataaacattt
    11341 tgaaaaaaaa atgcaagaat acgatgccag tcaaattcag gttctagaag ggctggaggc
    11401 tgtccgaatg cgtccaggga tgtatattgg ctcgacagct aaagagggtt tgcatcattt
    11461 agtctgggaa attgttgaca actcaattga cgaagcatta gcaggttttg cctctcatat
    11521 taaagtcttt attgaagcag ataattccat tacagtagta gacgatggcc gtggaattcc
    11581 agttgacatc caagccaaga caggacgtcc cgccgttgaa acagttttta cagtcttaca
    11641 cgcaggtggt aaatttggcg gaggcggcta taaggtttct ggaggactac atggtgtagg
    11701 gtcatctgtt gttaatgctt tatcaacaca attagatgtg cgtgtttata aaaacggcca
    11761 aattcattac caagaattta aacgcggggc tgttgtagca gatcttgaga tcattggaac
    11821 cactgatgtg actggaacaa cggtacactt tacacccgat ccagaaattt ttactgaaac
    11881 gactcagttt gattacagtg ttttagcaaa acgtattcaa gagttagcct ttttgaatcg
    11941 tggtttaaaa atttccatta cagataagcg ctcaggtatg gaacaagaag aacatttcca
    12001 ttatgaaggt ggaattggtt cttatgttga atttttaaat gataaaaaag atgttatctt
    12061 tgaaacgcct atctatacag atggtgaatt agaaggtatt gcagttgaag tagccatgca
    12121 atacacgact agctatcaag aaacagtcat gagttttgct aataatattc atactcatga
    12181 aggtggaacg catgaacaag gctttagagc ggctcttact cgggtcatca atgactacgc
    12241 taagaaaaat aaaattctta aagaaaatga ggacaatttg acaggagaag atgttcgtga
    12301 aggtttgacg gcggtaattt ctgttaagca tccaaatcct caatttgaag gtcaaaccaa
    12361 aacaaaattg ggcaactcag aagtggttaa gatcactaat cgtctcttta gtgaggcctt
    12421 tcaacgtttt cttttggaaa acccacaagt tgctcgtaag attgtggaaa aagggatttt
    12481 ggcttctaaa gctagaattg cagctaagcg agcccgcgaa gtcacccgca aaaaatcagg
    12541 cttagaaatt tcaaacttac ctggaaaatt agcagactgt tcgtcaaatg atgctaacca
    12601 aaacgaactt ttcatcgtcg aaggagattc agcgggtggg tcggccaaat caggtcgtaa
    12661 ccgagagttt caagctatct tgcctattcg cggtaaaatt ttgaacgtgg aaaaagcaac
    12721 tatggataag attcttgcca acgaagaaat tagaagtctc tttaccgcta tgggtacagg
    12781 ttttggtgca gactttgacg tgtcaaaagc tcgctaccaa aagctggtta tcatgaccga
    12841 tgccgatgtg gatggcgctc atattagaac cttgctttta actttgattt accgctttat
    12901 gaggcctgtt ctagaagctg gctatgttta catcgcccag ccacctattt atggtgttaa
    12961 ggtcggtagt gagattaaag agtatattca gccaggtatt gatcaagaag accaattaaa
    13021 aacagctctt gaaaaatata gtattggtcg ttcaaaacca actgttcaac gttataaagg
    13081 tcttggggaa atggatgacc atcaactttg ggaaactact atggatcctg aaaatcgttt
    13141 gatggcgcgt gtgacagttg atgatgccgc agaagcagat aaagtatttg atatgttaat
    13201 gggagatcgt gttgaaccaa gacgtgattt cattgaggaa aatgcggttt atagtacact
    13261 ggatatttag tcaccttttt agtttcacaa ggaatctgct accaaagagg acgaattgac
    13321 taaaaatcat gatgcatgat gccaacagct ttgttagtta gtacgtcttc atcttgaggg
    13381 acagcgttaa tgcataggta ttgagaccgc ccctttatac ctatgttagt ccttattaag
    13441 aaagccgtgt gagtcactgg tacgagattg ttgaattagt caaatatatt ttgaaatgca
    13501 ttttaaagta tgttataatc gtaatgttat agtgtatttt aaataaaatt ttaataaaag
    13561 gcgattgagg aatttcagtt tttagttatt actggaacaa tgttgataaa tcgtgtctaa
    13621 ataataagga gattcagatg tcaagcggaa ttatcttgct gattgtggct atagtcctac
    13681 tagtgattat cgcctatcta gtgggtgtca tcatacgaaa gcgtaatgat tccctaatta
    13741 ccagtctaga agaacgaaaa caggccttgt ttgctttgcc tgttaatgat gaaattgaag
    13801 aagtaaagtc acttcatttg ataggacaga gccaaacctc ttttcgggaa tggaatcaga
    13861 aatgggtaga cttaactgta aattcgtttg cagatattga aaatcatatt tttgaagctg
    13921 aaaatttgaa tgatacgttt aattttattc gcgccaagca tgagattaat agcgttgaaa
    13981 gtcagcttaa tcttgttgaa gaagatatcg cttctattcg tgaggcactt aatatcttaa
    14041 aagagcaaga agaaaaaaat agtgctcgtg tcacccatgc tcttgattta tatgaaaagc
    14101 tccaagcttc tatttctgag aatgaagata attttggttc aactatgcct gaaattgaca
    14161 aacagatgaa aaacattgaa actgaatttt cacagtttgt cgcactcaac tcatcaggtg
    14221 atcctgtaga agcttctgaa gttctggata gagcagaaga gcatactatt gctctagggc
    14281 agattacaga acagattcca gctattgtgg ccaagttgga agatgatttt ccagatcagt
    14341 tagatgattt ggaaacagga tatcgccgtt tacttgaaga aaactatcat tttccagaaa
    14401 aaaacattga ggcacgtttt caggagattc gtgagtctat tcgtgctaat tcatcagagt
    14461 tagttacact tgacttggat cgtgctagag aagaaaacac gcatattcaa gaacgcattg
    14521 attctcttta tgaagtcttt gagcgcgaaa ttgccgctta taaggtagct gctaaaaata
    14581 gtaagatgct acctcgttac ctagcacatg tgaaacgtaa taatgaacaa ctcaaagatg
    14641 aaattgcacg tttatcacgt aaatatattc taagtgaaac ggaaagtctg actgttaagg
    14701 catttgagaa ggatattaaa gagatcgaag acagtacact tgctgttgca gaacaatttg
    14761 gtttacaaga aaaaccattt tcagaactgc aagtgacttt tgaacgcagt ataaagacgc
    14821 ttactaatgt cgagtcaggt caaatggatg tttttgcagc agttaaagat attgaaaaaa
    14881 ttgaatcaca ggcacgccat aaccttgatg tttatgttac tcagcttcat atgattaaac
    14941 gttatatgga aaaacgtcac ttgcctggaa ttccacaaga ctttcttagt gctttcttta
    15001 caacgagttc acagttggaa gctttgatgg atgagcttag tagaggacgg atcaatattg
    15061 aggcagtgtc acgtttgtcg gaagttgcga cggttgctat tgctaatcta gaagatttaa
    15121 cctatcaagt ggttcaaaat gctaccttga ctgaacaatt actacaatat tctaatcgct
    15181 atcgttcttt tgaagcagga gttcaaagta gttttgaaca tgctttgaga cttttcgaag
    15241 tagaaaatga ttatcaggct tcttttgatg aaatttctta tgctcttgaa acagttgaac
    15301 ccggggtaac agatcggttt gttaactctt atgaaaaaac acgagagcac attcgttttt
    15361 gatacttgaa agtcgtgata ttctcacgac tttttattag ctttttggac caatacgtgt
    15421 ttttgactaa aatcttcttt ttaatgatca aaagaagaag tagtaacagt gttttttgct
    15481 attgagagga tagttatttt ttcaaaaatc ttttccaaaa ggatttggtt ttttcaggag
    15541 tttgtttgct aagttgatcg ttttttgtct gctttaagat gacttctaaa cgtgtttcct
    15601 tcagatttat tgcgtggtcg gtatgaagga taaacgcaaa aggtgaagtc atatgcgttt
    15661 ctgtaataat agtaacaggt aaatggtttt cttttgctaa tttcatatag agtatttgaa
    15721 gttgaggtgc cagcttggaa gagattttta agtgtaaagg ttgatagact aactgaaggc
    15781 tcggtaattc tcttttcaaa tagtctttta ctgttttagt ttgagcttcg tctaataaag
    15841 cacttaaaac gactcgttca gcatacgttc ctagatagta gcgttgctgc tcaggattta
    15901 atcttctttc tccccaggct ccttttaata gcttatcttc taaattagtc atagataccc
    15961 tcgcttcttt ataataataa tagtttcttt attatatcga tttctacaat aaaataccaa
    16021 tttgaaaatt ttcggtaaat gtgttaaaaa acaagaaaga aagtgatttt tcttgcaaaa
    16081 aacaaaggaa gcttttcatg agattgcttt ttaagttgat taaaggccta gtttgtggta
    16141 ttatgaacat gtaaaatatt aaaactcata aggagagtat tacaatgtca attattactg
    16201 atgtatacgc tcgcgaagtc cttgactcac gcggtaaccc aacacttgaa gtagaagttt
    16261 atacagaatc aggtgcattc ggacgtggta tggttccttc aggagcttca actggtgaac
    16321 atgaagccgt tgaacttcgt gatggtgaca aatctcgtta ccttggtcta ggtactcaaa
    16381 aagcagttga taacgttaat aacattatcg ctgaggcaat catcggttac gatgttcgcg
    16441 atcaacaagc tatcgaccgt gcaatgatcg ctcttgacgg tactcctaac aaaggtaaac
    16501 ttggtgctaa tgctattctt ggtgtttcta tcgctgttgc tcgtgcagct gctgactatc
    16561 ttgaagtgcc actttacact taccttggcg gattcaacac taaagttctt ccaactccta
    16621 tgatgaacat catcaacggt ggatcacact cagatgcccc aattgcattc caagagttca
    16681 tgatcatgcc agttggtgca cctactttca aagaaggtct tcgttggggt gctgaagttt
    16741 tccacgctct taagaaaatt cttaaagaac gcggacttgt tacagctgta ggtgacgaag
    16801 gtggatttgc tcctaaattt gaaggaactg aagacggtgt agaaactatc cttaaagcta
    16861 ttgaagcagc tggttacgaa gctggtgaaa acggcattat gatcggtttt gactgtgcat
    16921 catcagaatt ctacgacaaa gaacgtaaag tttacgacta tactaaattt gaaggtgaag
    16981 gcgctgctgt tcgtacatct gcagaacaag ttgattacct tgaagagttg gttaacaaat
    17041 acccaattat tactatcgaa gatggtatgg atgaaaatga ctgggatggt tggaaagttc
    17101 ttactgaacg cctaggcaaa cgtgttcaat tggttggtga cgacttcttt gttacaaaca
    17161 ctgaatacct tgctcgtggt atcaaagaaa atgcagctaa ctcaatcctt atcaaagtta
    17221 accaaatcgg tactttgact gaaacgtttg aagctatcga aatggctaaa gaagctggat
    17281 atactgccgt tgtatcacac cgttcaggtg aaactgaaga ttcaacaatc gctgacatcg
    17341 cagttgcaac aaacgctggc caaatcaaaa caggttcatt gtcacgtaca gaccgtattg
    17401 ctaaatacaa ccaattactt cgtatcgaag atcaacttgg tgaagttgct caatacaaag
    17461 gtatcaaatc attctataac ttaaaaaaat aggcggtaag acagaaatcg gtcattcgta
    17521 gaatgcgatt tcgtcgtcgc acctccgtca ggctgactag gttggtcacg acaaaaggag
    17581 tgaacatcca gtcagctacg acgcaaaagt taaagaatag aaagcttggg aaaatcccaa
    17641 gctttttgct ttctaaatag taaaaattta ctgtgggcta atttatcctg agttaatgat
    17701 tcgtgagaaa aacttaacaa cttgttaaaa aactttggtt ctgataatac aggaccaaag
    17761 tttttttatt tagtaaaaac gtcgtggtat aggtttgtcc aggtattaca tatttcaaag
    17821 tagtatctag gatgaagtaa agatattagc tagagttatc aaaatgatat aagttataaa
    17881 gctttgaaaa ttagctctca tttttgtcgc tattgtttga aaaatatagt tttttagaga
    17941 aaattattat tttttttgtc atttttgata atattaaaaa gaaagggttt acatattaat
    18001 cattt
//
LOCUS       DAUGFP010000031        17681 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.031, whole genome shotgun sequence.
ACCESSION   DAUGFP010000031 DAUGFP010000000
VERSION     DAUGFP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17681
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.031"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            407..1756
                     /locus_tag="VH663_001132"
     CDS             407..1756
                     /locus_tag="VH663_001132"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEQ8174339.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKADSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDN
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
     gene            complement(2105..3613)
                     /locus_tag="VH663_001133"
     CDS             complement(2105..3613)
                     /locus_tag="VH663_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8174340.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFLSGYWTS
                     HQRQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIANFITSTIPSYKVELSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRVPS"
     gene            4301..4972
                     /locus_tag="VH663_001134"
     CDS             4301..4972
                     /locus_tag="VH663_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEQ8174341.1"
                     /translation="MTRLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGVQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSSRFLLLYVL
                     SGVMGNAFTFWLTPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGALLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
     gene            complement(5071..5970)
                     /gene="galU"
                     /locus_tag="VH663_001135"
     CDS             complement(5071..5970)
                     /gene="galU"
                     /locus_tag="VH663_001135"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEQ8174342.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(6003..7019)
                     /locus_tag="VH663_001136"
     CDS             complement(6003..7019)
                     /locus_tag="VH663_001136"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ8174343.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     RNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            7317..7766
                     /locus_tag="VH663_001137"
     CDS             7317..7766
                     /locus_tag="VH663_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ8174344.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            7759..9465
                     /locus_tag="VH663_001138"
     CDS             7759..9465
                     /locus_tag="VH663_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174345.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPFLFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIEPGQMVGIVGATGSGKSTLVQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            9468..11252
                     /locus_tag="VH663_001139"
     CDS             9468..11252
                     /locus_tag="VH663_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174346.1"
                     /translation="MKTARFFWFYFKRYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTMTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAVVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTISQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            11370..12137
                     /locus_tag="VH663_001140"
     CDS             11370..12137
                     /locus_tag="VH663_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEQ8174347.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWAKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            12247..12693
                     /locus_tag="VH663_001141"
     CDS             12247..12693
                     /locus_tag="VH663_001141"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEQ8174348.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            12774..14135
                     /gene="radA"
                     /locus_tag="VH663_001142"
     CDS             12774..14135
                     /gene="radA"
                     /locus_tag="VH663_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEQ8174349.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            14324..14821
                     /locus_tag="VH663_001143"
     CDS             14324..14821
                     /locus_tag="VH663_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEQ8174350.1"
                     /translation="MSYFEHFMAANQVYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGVDVSGQDFLPFQDVEDSVRKDMAKLRASSLIPDDVVINGAVYDVDTGKM
                     TQVME"
     gene            14952..15662
                     /locus_tag="VH663_001144"
     CDS             14952..15662
                     /locus_tag="VH663_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEQ8174351.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            15844..17289
                     /locus_tag="VH663_001145"
     CDS             15844..17289
                     /locus_tag="VH663_001145"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEQ8174352.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
                     NEAGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
BASE COUNT         5386 a         3169 c         3773 g         5353 t
ORIGIN      
        1 agataatgag agtaagtaat gttaagaaga tagcatgaga aagtatttac aggtgattgg
       61 ggcttcaatt gcctttgtgg gtgtggcttt atgcattgat catatgcatg atggggttat
      121 tacccgtgtt gaaatgataa aatccttaaa ataagttatt acggtaaagc ggcctcaggg
      181 ccttttttca tgatattttt aggaaatatg aaatcctatt ttctcttaaa aaaaacacta
      241 tgatgagaat atgcttgcat tgacacatgt tttatgtctg aaaaaacgaa aaccttttca
      301 aaaaacctag ctttttcaca ggtgaaaacc actaaaaacc attgaaatat tagatagaaa
      361 aggttatcat actaatgaaa cttaaaattt taagggagcg aaactcatgt cacatattac
      421 atttgattat tcaaaagtcc tcgagtcatt tgcgggacaa catgaaattg attttttaca
      481 aggccaagtc acagaagctg acaagttatt gcgtgaagga actggaccag gttcagattt
      541 cttgggttgg cttgatcttc cagaaaacta tgacaaagag gaatttgcac gtatcttgac
      601 tgctgctgaa aagattaagg cagacagcga agttcttgtg gtgatcggta tcggtgggtc
      661 ataccttggt gccaaagcag cgattgattt tttaaatcat cattttgcta acttgcaaac
      721 tgccaaagag cgcaaagccc cacaaattct ttacgctgga aactctattt catcaactta
      781 cttagctgac cttgtagagt acgttcaaga caaagaattc tcagtaaacg tgatctctaa
      841 atcaggtaca accactgagc cagctattgc ttttcgcgtg ttcaaagaac ttttggttaa
      901 aaaatatggc caagaagaag ctaacaaacg tatctacgct accacagaca aagttaaggg
      961 tgctgttaag gtagaagctg acgctaacaa ctgggaaaca tttgtggtgc cagataatgt
     1021 tggtggtcgt ttctcagtct tgactgctgt tggtttgctt ccaattgctg catcaggtgc
     1081 agatattact gctcttatgg aaggggcaaa tgctgctcgt aaagaccttt cttcagacaa
     1141 aatctctgaa aacattgctt accaatacgc tgctgttcgt aatgtgcttt accgtaaagg
     1201 ctacatcact gaaatcttgg ctaactacga gccatctctt cagtactttg gcgaatggtg
     1261 gaaacaatta gctggtgaat cagaaggaaa agatcaaaaa gggatttacc caacttcagc
     1321 taacttctca acagaccttc actcacttgg tcaatttatc caagaaggtt accgtaacct
     1381 ctttgaaaca gttattcgtg ttgataaccc tcgtaaaaat gttatcattc ctgaattagc
     1441 agaagaccta gatggtcttg gttaccttca aggtaaagac gttgattttg ttaataagaa
     1501 agccacagat ggtgtgcttc ttgcccacac agatggtggc gtgccaaaca tgtttgtcac
     1561 tcttccagct caagacgagt ttacccttgg ttacactatt tacttctttg agcttgccat
     1621 cgctgtttca ggctacatga atgctgttaa cccatttgat caaccaggtg ttgaagctta
     1681 caaacgtaat atgtttgcgc ttcttggaaa accaggtttt gaagcattat cagcagagct
     1741 taatgctcgc ctctaatcaa cagatagtca aaaagacttc ttccttgtac aagagcttaa
     1801 aagttagcca agcaatctaa gaattggggc ggcacttcca tcatcaagtg gatacaaggt
     1861 taagtaaaag cgataataaa gtgcgcttcc agaacatggg agcgcacttt attatgccaa
     1921 atttcagata aaaccttcaa aaaagggaag aagatcctac aagaggagtt gaaccgctaa
     1981 aacagttcca gttctgtttc taagcaaata gcaatggtta aaaagagagc ttatcaaggt
     2041 tgcaagtgac aaccttaaag acccccctag actgtgaaag tatctaggag ggactgtcaa
     2101 aaaattaaga aggaactctc caaagttctt ctaaacgttt ttggtagtgg ttctggtgca
     2161 aggctaagat ggttttgatt aaatcttcgc gctgctgttg gatacagtcc aaactcaaac
     2221 caaacaagcg ggtctctttg ggaaaaacac cgagttcttt taagaaaggc aagagcttct
     2281 gactggtcac aaccagatct acaggtttat catagggata aatattatca gataagatat
     2341 tgacccgtga cagttcaact ttgtaagaag ggatagttga ggtgatgaaa ttagctataa
     2401 cgttaccaac agtttcttgg ctctcaataa cagtaatatt gattggagca atagagcttt
     2461 ccaagatctg ctcaaggtat aaacacatca agtgtaagtg accagaacta atactgcctt
     2521 gacggcctgt gtcataaagc caatctttga taacggtttc aagcttatct agtagtagca
     2581 tgttgccttg atattgatca aaataataac catcaaaggt aattaaagtt tgtagattgt
     2641 agagagttct tttgaaaaaa ggaattaagt tagggatatg ctcatgaata tcggaatact
     2701 ttccaactag tgcctgaaga cgatggatta gtaaatgata atcgggatct ttggtgatta
     2761 aatggtaaag ttgttgtcgc tgatgacttg tccagtaccc actcaagaag gagttatcag
     2821 tcgttaaata aattaaaaag agatagtcaa aatcatcaga cgaaaatgtg acacgagttc
     2881 gaggtgctag ctcatccacg gcaatggttt tgatattgtc aaaaataggc aaggatttga
     2941 gatgttcaaa aagagctaaa tgaggcaaat gaacaggata gcgatggcgc ttccaagtca
     3001 acatcaacag cacatcaaaa aacaagaagc gtctatctaa aaaggcagaa ggttgtaagt
     3061 ttttttgtgc tgcaaagata aaagcatgaa tcgtttcaat gtctgcaggt tgtatatcat
     3121 ataatataat gccgtactct ttatgtaaaa gagcaatcaa ataccgtatt ttaagttcat
     3181 ctccgataac agtattgtct acaatattta aaccaatgtc ttgtaaataa tgttttaatt
     3241 tatgaataag acgataagct tttgactggg atatcccttg ttttcgtgta taaacagcga
     3301 ttgacttgtg tttgggatta tctagtaata agaatgtcaa catttttaga gtatctgata
     3361 aggcaaaaat atcataaaaa aatgcttctc gcttatcatt taatacctca caataaatac
     3421 gagatttaat aagctcaata cgtaagtacc ctttaaataa ggcgttaaat tgtcgaagat
     3481 actgctttat ttttaaagca gtcaaagttg tttgagaaca agctgctgcc atcgtcagtt
     3541 ctttttctgt caacaaaata gccagtagga ttttctggtc aataatatcg gattccaaat
     3601 aataatctaa caatgtaatt tttctctcct taaattaaat tgtataatcc tatatattgt
     3661 agcgaatcta ccgtaaaagt caactaaaat caataggatt actaatatag tcacaataaa
     3721 gatcatcaaa aagcagcaaa cttcatcaat agattgattg atgcgtgttt ttagcccata
     3781 attgagagag ctaagaacac gcgggtgata tcatattttt gctgttgact ttttaaattt
     3841 aaaaacctaa ccagccggtt aactggtcag gtttaaacaa gggagggcac ctaagaagat
     3901 aggtgcccag gagatttatg aaaagggaaa acaaggggaa aacctattct cccaacggtt
     3961 taggtaaacc gctttttagg atgttttcat tctacgatag taaccttaaa gttttcttaa
     4021 atgattcttt agaatgcctt tatttttaat cttttttgag agagctagct tcttgtcagc
     4081 cttgaacctt tttcctaatt tatggtatga taagaagata atgaaaaaag agattctcaa
     4141 agaactcttc tagtctttac gaactatgga tgtagactta aaacgtcaaa atgatcaagc
     4201 tttattagtg gatgttgtta gcaatcaagc ttatcaacgg gccaatacga ttgccaaatc
     4261 aaacgattta tgatattgct gtttaggagg tttatcttag atgacacggt tattaaaacg
     4321 atatcctatt actattttcc ttttagggtt aacaggctta atttttatag caatgcaagt
     4381 ggtatatgga catttagcaa ctggtgttca agctatttat caagttggag gtatgtttgg
     4441 attactagta aaagcaatgc cagatcagtt gtggcgattg gtgactccga tatttattca
     4501 tatcggtttt ggtcattttt ttgtcaatgg attaacacta tattttgttg gtcaaatcgt
     4561 cgaagacctc tggggctcaa gccgtttttt attactttat gttttatcag gtgttatggg
     4621 caatgccttt actttttggt tgactcctga aacagtagct gcaggagcat cgacgtcgtt
     4681 atttggactt tttgctgcta ttgtagtatt gagttttttg ggtaagaacc aagcgttgaa
     4741 agatttgggc aagtcatacc aaacgttaat tgtagtcaat ttactgatga atctttttat
     4801 gccaaacgtg agtatggcag gccatattgg cggtgtcgtg ggtggtgcgt tactgagtat
     4861 agtatttcca actaagatga gagtgataac agtgaagaaa accaagcgaa tgttggcttt
     4921 agtgagctat ggtattattt tggttggtgt gttagtgctt gggtttttat aaaaataagt
     4981 gagtggttag cactgttact tctcagatgg ctagtaggat gaaacgactc aaaaaccata
     5041 aaaataagga gggattttcc ctccttattt ttatggtttt gttttctcta gtgctttgcc
     5101 cagttggatg atgtaggctt tcaagtcatc tttgacttga gggtgtttca gggcataatc
     5161 caaagaagtt ttcataaacc caaatttatc gccaacatca tagcgtttcc ctttaaactc
     5221 acgagcaaag acacgctgtg tcttattgag ggtatcaata gcatcagtca gttgaacttc
     5281 attgcctgca ccaggttctt gtttttctaa aatactaaaa atttctggtg tgagcaagta
     5341 acgtccaata attgccaaat cactaggtgc atcttgaggt tgtggttttt caacaaaagt
     5401 atccacacta tagagtcctt ttactgcttt tgcttgagga gcaatgatac cgtagttaga
     5461 gacatcctcg tgaggaaccc tcatgacggc aatagttgca gcatgagttt cttcatagtc
     5521 atcgatcaat tgtttggtta atggttttac atttggattt gtaatatcca tcagatcatc
     5581 acctagcatg acaacaaaag gttcattgcc aacaaaggtc tttgcttgaa gaactgcgtc
     5641 tcctaatcct cttggatgac tctgtcgaat aaagtgaaga ttaatagaag tggtctcgtc
     5701 tactaacttt agaagttcga tttttccttt agcctgtaaa ttatattcta attcaaaatt
     5761 agaatcaaaa tgatcttcta tggagcgttt ggattttcca gtaacgataa gaatttcttc
     5821 gatacctgat tttagagctt cttcaacgat aaattgaata gttggtttat caacaatagg
     5881 tagcatctcc ttggcaaggg ccttggttgc aggtaaaaaa cgtgtgccta aacctgctgc
     5941 gggaataata gcttttctga ctttggtcat cagcacctcc ttttatttta gtgacgcatg
     6001 gcttaatgcc attcattttc agaacgaaat tcatttgaca tcatgccgag gatgctttct
     6061 ttgatatcag cgccctcgta gatggactta taaatagctg ttgtaattgg catgtaaact
     6121 ccgaggtctt gggcaatttc ataggctacc ttagttgttg cgatgccttc gataaccata
     6181 cccatgttgc gttcaatatc ttctaatttt tcaccacgtc caagggcagc tccagcccgc
     6241 cagttacgtg aatggacaga agtacctgtt acaattagat caccaacacc agataaccca
     6301 ctgtaggtta acgggtcagc accaagcttg acgcctagac gggtgatttc agctaaccca
     6361 cgagtaatta ccgctgcttt ggcattatct ccatacccaa gtccatggag agctcctgct
     6421 ccaacagcaa taatgttttt gagggcacca gctgtttcaa ctccgatgac gtcggtattg
     6481 gtataaagtc ggaagtagtg gttactaaag agggactgaa catactttgc agcttcaata
     6541 tcttttgagg ctgccgtgat gagagtaata tcgcgaacga tagtttcttc agcatgacta
     6601 ggacctgata caacgaccac ttcactacga aaatgagctg gaatttcttc ttctaggata
     6661 gttgataaac gttcgtgggt ctctggttct agaccttttg aggcatgcat gaccactact
     6721 ttatgatcta aaatggcggc aacttgtcta gcaactaatc gtgtcacttt agttggcaca
     6781 acaaaaagca ccgcatcaac atctgatagg gcttgaccta aatcaagagt tgccgtaatg
     6841 tttttgtcta acacaatgtc tttgaagtag tgtctattgg tatgcttggt atttatctct
     6901 tcaatttgat caggaatatt tccccataaa cggacgtcat ggccattgtc attaagaacc
     6961 tgagaaaggg cagttcccca agaaccaggt cctaaaatag ctactttttg ttttgtcatt
     7021 ctaaattctc cttcaagagc tagtcactct attataacat aacaccttga ttagctctat
     7081 tagaaaaggt atatggtctt ggctaataag gcgttcccaa gagagctagc tatattaata
     7141 atgttgttta ataagcttca tcaggtgagc tgttgtcatg gctttgccct gttcttttta
     7201 gttatttaag ttcggaaact tatttttaaa aataaagata ttttaattga caatagttat
     7261 ctagagaact aaaatactta tcatagaaac tattaaagaa aataaggagg gaagagatgt
     7321 cacaagtgat aggtgattta cgtgaattga tacatcaaat cgaacaaatt agtgatgaga
     7381 ttgcaaaaaa atatgatgta gagcatctag caggtcctca aggttatgtt cttgtttttt
     7441 tagctaaaca ccaaaatcaa gaaatatttg tcaaagatat tgaaaaacaa cttcgtatct
     7501 caaagtcagt tgctagtcat ttagtgaaac gtatggtcaa aaatgggttt atcaatgtga
     7561 tgccttccca agtggataag cgttataagc aagtagtgtt agcgcaggtt ggtagagata
     7621 aattgccttt gttgcgggag tgtcgtaagg atatcgagca ctatttttta aaagaaatta
     7681 caaaagaaga gttgctgaca gcgaaaaaag taattgaaca gctcaagcaa aatatgctaa
     7741 cttataaagg agacaacgat gcttaaagac gcaatcttac gttataaatg gtatgcttta
     7801 ggctcatttt ttatggttat tggggttgtt gcaagtagtc tcttgcaacc acattatctt
     7861 aaatctgtgc taagcgctgt tattcaaaat aatcaagaga aaatttatga ggttgggaaa
     7921 ccattactta ttattgccct tttagggtta atgtctggag cgattaatac ggttttagct
     7981 gctaaaatag ctcaaggtgt atctgctgat atgagggaga agacctttcg aaaaatccaa
     8041 gacttttcat atgccaatat tgaagctttt aatgctggta atcttgtggt taggctaact
     8101 aatgatatca atcaaatcca aagtttagtc atgatgatgt ttcaaattct ctttcgtttg
     8161 ccgttccttt ttattggcgc gtttatcatg gcagttcaga cattccctca attatggtgg
     8221 gtcattgttg taatggttat cttaattgcc ctcatcatgg gtttggtgat gcgtcaaatg
     8281 ggtcctcgtt ttggtaaatt tcaacggtta atggataaga ttaatcgcat cgctaaggaa
     8341 aatctccgtg gcgttcgcgt ggttaaatca tttgtacaag aacaacaaca atacactaaa
     8401 tttaaagaaa cgtccaatga tcttttggcg ttaaatttat ctattggtta tggtttttca
     8461 ctaatgcagc cggctttaat gttggtgtct tatttggcag tttatgtttc tataaatgtt
     8521 gtttcgacca tggtcgaaac agatccaaca gtgattggga atattgcctc tttcatgact
     8581 tatatgatgc aaattatgtt ttcaattatt gtggttggtt ccatggggat gcaagttagt
     8641 cgtgcttttg tttctatggc tagaattagg caaatattgt ctacagaacc tgccatgaca
     8701 tttgaaaatg aaaaggaaga aaccatctca ggaagcattg tatttgacga tgttagtttt
     8761 acatatccta atgatgatga gccaaccttg aagcacattt cttttgctat tgaacctggg
     8821 caaatggtcg gcatcgttgg tgcaacagga tctgggaaat caacgcttgt acagctcatc
     8881 cctcgcttat ttgatcctca agatggccaa attttgttag gaggaaagcc gattaaaacc
     8941 ctaagtcaaa caaccctgcg ccaatcggtt tccatcgtgt tgcaaaaggc tattctcttt
     9001 tcaggaacga tagctgataa ccttcgccaa ggctcagcaa aagctgatat tgatgctatg
     9061 caaaaagccg ctcaaatcgc gcaagcaaaa gaatttattg atcgcatgga tagccgttat
     9121 gagagccaag ttgaagagcg aggtagtaac ttgtctggcg gacaaaagca acgtctgtct
     9181 attgcacgtg gtgtcattaa tcatcccaaa atccttatat tagacgattc cacttcagct
     9241 cttgatgcaa aatctgaaaa acgcgttcaa gaagccttaa gtcataaatt agaaggtaca
     9301 actacagtta ttgttgctca aaaaatttca tcggtagtta aggcagataa gatcttagtc
     9361 cttgaccaag gacagttaat tggagaagga acacacgcag agttggtggc taataatgcg
     9421 atttatcgtg aaatttatga gacacaaaaa ggaagggagg agtagtgatg aaaacagcac
     9481 gttttttctg gttttatttt aaacgctatc gtttctcgtt tactgtcatt gctgttgccg
     9541 ttatcttagc aacttattta caagtaaaag ctcctgtctt cttaggggag tccttgactg
     9601 agttgggaaa aatcggtcag gcttattacg ttgctaagat gagtggtcag acacatttta
     9661 gccctgattt atcagctttt aatgccgtga tgtttaagct tttgatgact tatttcttta
     9721 ctgttttagc taatctaata tatagtttct tactcacacg tgttgtctca cattcgacca
     9781 accgcatgcg caagggcttg tttggtaaat tagaacgttt aaccgtcgcc ttttttgacc
     9841 gccataaaga tggggagatt ctttctcgtt tcacgagtga tttggataat attcaaaact
     9901 cgctgaacca atccttggtt caagtggtga ctaatattgc cctttacatc ggcctggtct
     9961 ggatgatgtt taggcaagat agccgtttag ctttgttaac catcgcatca accccagttg
    10021 ctctcatttt tttagtgatt aacatccgtt tggcaagaaa atacaccaat atccaacagc
    10081 aagaagtcag tgctttaaat gcttttatgg atgaaaccat ttcaggacaa aaggctatta
    10141 ttgtacaagg tgtccaagaa gatacgatga cagccttttt aaagcataat gaaagggttc
    10201 gacaagccac cttcaaacgc cgtctgttct caggacaatt atttccagtc atgaatggaa
    10261 tgagccttat taacacggct gtcgtgattt ttgtcggttc aacaattgtc ctcagtgaca
    10321 aatctatgcc agcagcggca gcgcttggtt tagtggttac ttttgtacaa tattcccagc
    10381 aatattacca acccatgatg caaatcgcgt ctagttgggg agaattgcag ctggccttta
    10441 ccggtgctca ccgtattcaa gaaatgtttg atgaaaccga agaagttcgt ccacaaaatg
    10501 caccagcgtt caccagctta aaagaagcag tggcgattaa ccacgtcgat tttgggtatc
    10561 ttcctgggca aaaagtttta tcagatgtgt caatcgttgc acccaagggc aaaatgattg
    10621 ccgtggttgg accgacaggt tctggaaaga ccactattat gaacttgatt aaccgtttct
    10681 acgatgtgga tgcaggttcg attacctttg atggccgtga tattcgtgac tacgatttgg
    10741 atagtcttcg tcaaaaggta gggattgtgt tgcaagagtc agttcttttt tcaggaacca
    10801 ttacggataa tattcgtttt ggtgatcaga ccattagtca agacatggtt gaaactgctg
    10861 cgcgtgcgac ccatattcat gactttatca tgtccttacc aaaagggtac aatacctatg
    10921 tctcagatga tgacaatgtc ttttcaacag gtcaaaagca gttgatttct attgctagga
    10981 cgctactgac ggaccctgaa gtgttgattt tggatgaggc cacttcaaat gttgatacgg
    11041 ttaccgaaag taaaattcaa cgggccatgg aagctatcgt ggcaggtcga accagctttg
    11101 tcattgctca ccgcctcaaa accattttaa atgccgatca cattattgtg ttgaaagatg
    11161 gcaaggtcat tgagcaagga aatcatcatg agctattgca tcaaaaaggc ttttatgccg
    11221 aattgtatca caatcaattt gtctttgaat agatggcatc agctccgctt cttagcggag
    11281 ttttttggtg aacaataaag cagtcaagct ttctttcctg atatggtata atagcaagca
    11341 agagaaaaaa caattagaaa gtagccacaa tgattgattt acaagagatt ctcgctaaca
    11401 tgaatcccaa tcaaaagata aattatgaca gggtcatgca acagatggcc aaggtttggg
    11461 ctaaggaatc cgtgcgtccc agtattttaa tgcatgtctg ctgcgcccca tgctccactt
    11521 acaccctaga atacctgacg cagtttgctg acattacggt ttactttgcc aattctaata
    11581 ttcaccctaa ggacgaatac catcgcagag cctatgtgac acaacagttt gtctccgagt
    11641 tcaacgctaa aacaggcaac accgtgcagt ttctcgaagc ggactatgtc cccaatgagt
    11701 acgttcgaca agtgcgaggt ttagaagaag aaccagaagg tggggaccgt tgccgtgtct
    11761 gttttgatta ccgactagac aagaccgctc aaaaagcagt ggaactaggt tttgactact
    11821 ttgccagtgc cctaaccatc agtcctcata aaaattcgca aaccattaat gatgtgggca
    11881 tcgatgtgca aaaggtttac accaccaaat acctgccaag tgatttcaag aaaaacaacg
    11941 gttatcgcag atccgttgaa atgtgtgagg agtatgatat ttaccgtcaa tgctattgtg
    12001 gctgtgtcta tgctgccaaa atgcaaggta ttgacttggt tcaagtgaaa aaagatgcca
    12061 aagcttttat ggcagacaag gatttggata atgatttcac ccacatccgc tttagttatc
    12121 gtggggatga gatgtgatgg catgattaaa agtggtccgt ttaaagttga ccgctttttt
    12181 gctattcttt tacgaatagt ctataataag aggacatgat tgacttgaaa ggatatacct
    12241 atcataatga caaaaattcg tggtttcgag cttgtttcaa gctttactaa tcctgattta
    12301 ctaccaaaac gtgagacgac tcatgcagct ggttatgacc tgtctgttgc tgaggctgtg
    12361 accattgccc caggggagat taagttggta ccaacagggg ttaaagccta catgcaagat
    12421 ggcgaggtgc tttacctcta tgatcgttcg tcaaatccac gaaaaaaagg gattatcctg
    12481 attaattcag taggtgtgat tgatgcagat tattatggca atgaggctaa tgaaggacat
    12541 atttttgcac aaatgcaaaa cattacagat caccctgtga cattagctgt gggtgagagg
    12601 atcgttcagg gggtttttat gcctttttta attgctgatg gtgaccaagc cagaggtgaa
    12661 cgaacaggtg gttttggttc gacagggcag taaagcgatt taacaaaaat gcttatgaca
    12721 gcagacctat tctttaagag tgggtttgtc ttgtctaggg gagggagatt actatggcaa
    12781 agaaaaaagc aacctttatc tgtcaggagt gtggctacca gtcgccaaaa tatttagggc
    12841 gctgtccaaa ttgctcggcc tggtcttctt ttgtggaaga agtagaggtc aaagaagtca
    12901 aaaatgcccg tgttagtcta gctggagaaa agtccagacc ggttaaacta aaagatgtgg
    12961 ataatattag ttatcacaga acacagactg acatgtctga atttaataga gttcttggag
    13021 ggggtgtggt tcctggaagc ttgattttaa ttggtggtga tccgggtatt ggaaaatcca
    13081 ccttactctt gcaggtatcc acgcagctcg ctaataaggg aacggttctc tatgtgtctg
    13141 gggaagaatc agcagagcag attaaactgc ggagtgagcg tctgggtgat attgacaatg
    13201 aattttacct ctatgccgaa accaatatgc aagctattcg cacagaaatt gaaaatatta
    13261 aaccagattt tttaattatt gactccattc agaccatcat gagccctgat attacaggtg
    13321 ttcaaggatc agtcagtcag gtgagagaag tcactgcaga attgatgcag cttgcaaaga
    13381 cgaacaatat tgcaaccttt attgtgggtc atgtcaccaa ggaaggaacc ttggctgggc
    13441 ctcgtatgtt agaacacatg gtggataccg ttctctactt tgaaggggaa aggcatcaca
    13501 cctttcgcat tcttcgggct gtgaaaaacc gctttggctc cactaacgag atcggcatct
    13561 ttgaaatgca gtctggcggc ttggtagagg tcttaaatcc tagtcaagtc tttttagagg
    13621 aacgcttaga tggggcaaca ggatcggctg tcgttgtaac gatggaagga agtcgtccta
    13681 tcttagcaga ggtgcaatct ttagtgacac caaccgtttt tggcaatgcc agacgaacca
    13741 ctacgggact tgatttcaat cgcgtaagcc taataatggc agttttagaa aagcgctgtg
    13801 gtttactctt acaaaatcaa gatgcctacc tcaagtcggc tggtggtgtc aaactagacg
    13861 aacctgccat tgatttggca gtcgctgtgg ctatcgcatc cagttataag gaaaaaccaa
    13921 ccagccctca agaagctttc ttaggcgaga tcgggttaac aggtgagatt cggcgagtga
    13981 cccgtattga gcaacggatt aatgaagcag caaagcttgg atttaccaag gtctatgctc
    14041 ctaaaaatgc cttgcaaggc attgatatcc ctcaaggtat tgaggttgtc ggagtgacta
    14101 cggttggtca ggttttaaag gcagttttta gctagaggat cagaaactaa gagcaaatga
    14161 ggaggaaatt tgtcaagaaa gtatcccaat catttgtttt ttaaactttt cgaacgatac
    14221 cgaccgtcta agaggttaat ccagctattt tccataaaag atgttaagat aagacaaact
    14281 aacagcaaaa atagctctgc tgtttctcaa aaggagttta ttgatgtctt attttgaaca
    14341 ttttatggct gccaatcagg tttatgtggc cttgcatggg acagctcatt tgcccttaaa
    14401 acccaagact aaagtagcga ttgtgacttg tatggattca cggctacatg tggcccaagc
    14461 tctggggctg gctctaggag atgctcatat tttaaggaat gctggcggac gtgtcaccga
    14521 agacatgatt cggtcgctgg ttatttctca gcagcaaatg ggaacgcgag agattgtggt
    14581 tctccaccat acagattgtg gcgcgcagac ttttactaat gaaggctttg ccaagcatat
    14641 tcatgagcac ttaggtgtgg atgtttcagg gcaagatttc cttcccttcc aagatgtgga
    14701 agacagtgtc cgcaaggata tggctaagct aagagcctcc tctcttatcc ctgatgatgt
    14761 cgttattaac ggtgccgtct acgatgtgga tactggaaag atgacacaag taatggaata
    14821 gttagtagcc aagttgtaga gctgaattca gctctttttt atttttaaaa attattgaaa
    14881 atattagaca aagaaatagg gaagcgttat aataattgta tcaagttatt catacaaaca
    14941 aggagagtaa gatggtcgac aatagaatga gatttacgat tgatcaaaac atgcaatttc
    15001 cactagttga gattgatctg gaacatgggg gatcagttta cttacaacaa ggaagtatgg
    15061 tttatcatac tgagaatgta accttgaata ccaaattgaa tggtaaggga tctggtttag
    15121 gaaaattagt tggagccatc ggccgttcca tggtttctgg tgaatcaatg ttcattacac
    15181 aagctatgtc aaatggagat ggtaaattag cattagcacc caatacacca ggacagattg
    15241 ttgctttaga actaggtgaa aaacaatatc gtctaaatga tggtgccttt ctagccctag
    15301 atggttcagc acagtacaaa atggaacgtc aaaatattgg aaaagcactt tttggtggtc
    15361 aaggcggact ttttgtcatg acaactgaag gccttggtac cttacttgct aactcgtttg
    15421 gatctatcaa gaaaatcaca cttgatggtg gcacaatgac tattgataat gcccatgttg
    15481 tagcatggag cagagaactt gattatgata ttcatttaga aaatggcttt atgcagtcaa
    15541 ttggtacagg tgaaggagtt gttaatacct ttagaggtca tggtgagatt tatatccaaa
    15601 gtcttaacct tgagcaattt gctgggactc ttaaacgtta tctaccaact agttcaaatt
    15661 agtcaaaata acataaaatc agctttttgg ggctgatttt tttcgcctaa gcaagtaaaa
    15721 tatgctataa taagaaagat tttagaagaa gtctgaaaag gtcttggcaa gctcaaatca
    15781 tcatttgttg cttgtttttc gtgccacaga tttctgagtg atatatgtaa caggagaaga
    15841 agcatgtcta aaccaattcg tgtgcgttat gcaccaagtc caacagggct tttacatatc
    15901 gggaatgccc gcacagccct atttaattac ctttatgccc gtcgtcacgg aggtactttt
    15961 atcattcgta ttgaagatac tgaccgtaaa cgtcatgttg aagatggtga acgttctcaa
    16021 cttgaaaacc taaaatggtt aggaatggat tgggatgaaa gcccagaaac gcatgaaaat
    16081 taccgccagt ctgaacgtct agcactttat caacaatata ttgatcaatt gttagctgaa
    16141 ggaaaagcct ataagtctta cgtgactgag gaagaattgg cagcagagcg tgaacgccaa
    16201 gaagcagctg gcgaaacgcc tcgctatatc aatgaattta ttggtatgtc agctgacgaa
    16261 aaagctaagt acattgctga gcgtgaagca gcaggtatcg ttccaacagt ccgtcttgct
    16321 gttaatgaag ctggtattta caaatggacg gacatggtta aaggcgatat tgagtttgaa
    16381 ggtggcaata tcggtggtga ctgggttatt cagaaaaaag atggttaccc aacttacaac
    16441 tttgcggttg ttgtagatga ccatgacatg caaatttcac atgttatccg tggggatgac
    16501 catatagcca atacgccaaa acaattgatg gtctatgaag ctcttggctg ggaagcgcca
    16561 gagtttggtc atatgactct tatcatcaat tctgaaacgg gtaaaaaatt atcaaaacgc
    16621 gacaccaata ccctccaatt cattgaagat taccgcaaaa aaggttatat gcctgaagct
    16681 gtctttaact tcatcgccct tcttggctgg aatcctggcg gagaagagga aatcttctct
    16741 cgtgagcaac tgattgccct ttttgatgaa aatcgcctta gcaagtcgcc agctgccttt
    16801 gaccagaaga aaatggattg gatgagcaat gagtatctta aacatgcgga ttttgaaaca
    16861 gtttatgccc tctgcaaacc tttccttgaa gaggcaggcc gtttgacaga aaaagctgag
    16921 aagctagtag aattatacaa acctcaatta aaatctgcag atgagattat tccattgact
    16981 gaccttttct tttctgattt ccctgaatta acagaagccg aaaaagaagt gatggcaggt
    17041 gaaacagttt cgacagtctt gcaagctttc aaggctaagt tggaagctat gtcagatgaa
    17101 gacttcaaac cagaaaatat tttcccacaa attaaggctg ttcaaaaaga aacaggaatc
    17161 aaagggaaaa acctctttat gcctattcgt attgcagtat ctggtgaaat gcatggccca
    17221 gaattaccaa atacgattta tttattggga cgtgataaat ccattgagca catcaaaaac
    17281 atgctataag gctgtatcgt tcacctttat ttttaaacgc tttaaggagt taaaaagtca
    17341 cgacaacgaa agattgaaga ttaagttctt cagtcttttt tatcgttatc tgagcttgct
    17401 accttggtaa agtaaaaaat agtatagttt atttctattt tggagattgg atttgaaaga
    17461 gaggtggtga gatgcttacg tgataatgac agcaaacggt gaggttttga ttagtgtgat
    17521 ttaaagaaat ggtttttatg aatcaattag tgtacaatat ttttttggta tatgctgaaa
    17581 gagactagtt tagtattcac ttagctaatc aattgtaatc aaataatatt gtaatcattg
    17641 tcataaaata tgctaaaata ataaagttgg aaaggagaaa a
//
LOCUS       DAUGFP010000032        17273 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.032, whole genome shotgun sequence.
ACCESSION   DAUGFP010000032 DAUGFP010000000
VERSION     DAUGFP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17273
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.032"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            106..311
                     /locus_tag="VH663_001146"
                     /pseudo
     CDS             106..311
                     /locus_tag="VH663_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(382..525)
                     /locus_tag="VH663_001147"
     CDS             complement(382..525)
                     /locus_tag="VH663_001147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174353.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            612..833
                     /locus_tag="VH663_001148"
     CDS             612..833
                     /locus_tag="VH663_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEQ8174354.1"
                     /translation="MNIGFLEHTARNIIREAKRIAIKKFEEAREIDQNAVQLSKSPFD
                     NRRLGIAPKDIVEELVGILLSEESLESEN"
     gene            836..2023
                     /locus_tag="VH663_001149"
                     /pseudo
     CDS             836..2023
                     /locus_tag="VH663_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106750.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2181..3416
                     /locus_tag="VH663_001150"
     CDS             2181..3416
                     /locus_tag="VH663_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ8174355.1"
                     /translation="MIKRWAILFFVVLTCSGLSKIVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDRVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVDRTYCRRKILKKGSLVSERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(3532..4494)
                     /locus_tag="VH663_001151"
     CDS             complement(3532..4494)
                     /locus_tag="VH663_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ8174356.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMSPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEIGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            4827..6104
                     /locus_tag="VH663_001152"
     CDS             4827..6104
                     /locus_tag="VH663_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ8174357.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLQDRYP
                     RLTPDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIVDMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRRYWKKHIEKSVLLTH"
     gene            6139..6723
                     /gene="folE"
                     /locus_tag="VH663_001153"
     CDS             6139..6723
                     /gene="folE"
                     /locus_tag="VH663_001153"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEQ8174358.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEHHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKQPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            6732..7532
                     /gene="folP"
                     /locus_tag="VH663_001154"
     CDS             6732..7532
                     /gene="folP"
                     /locus_tag="VH663_001154"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEQ8174359.1"
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESARPGCQFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWIDPGFGFAKSVQQNTELLKGLDRVCQLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
     gene            7539..7898
                     /gene="folB"
                     /locus_tag="VH663_001155"
     CDS             7539..7898
                     /gene="folB"
                     /locus_tag="VH663_001155"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEQ8174360.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGGKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            7895..8395
                     /gene="folK"
                     /locus_tag="VH663_001156"
     CDS             7895..8395
                     /gene="folK"
                     /locus_tag="VH663_001156"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEQ8174361.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKLLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            8545..9432
                     /gene="murB"
                     /locus_tag="VH663_001157"
     CDS             8545..9432
                     /gene="murB"
                     /locus_tag="VH663_001157"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEQ8174362.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     VYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSVGGAVFMNAGAYGGEISHIFLSAKVLTSSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVADGTAKDYEDLIAYVIETV
                     ENHSGVRLEPEVRIIGENL"
     gene            9478..10632
                     /locus_tag="VH663_001158"
     CDS             9478..10632
                     /locus_tag="VH663_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174363.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            10616..11410
                     /locus_tag="VH663_001159"
     CDS             10616..11410
                     /locus_tag="VH663_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174364.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            11407..12183
                     /locus_tag="VH663_001160"
     CDS             11407..12183
                     /locus_tag="VH663_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174365.1"
                     /translation="MKKFANLYLASVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            12176..13249
                     /locus_tag="VH663_001161"
     CDS             12176..13249
                     /locus_tag="VH663_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ8174366.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKVPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDGIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(13304..13969)
                     /locus_tag="VH663_001162"
     CDS             complement(13304..13969)
                     /locus_tag="VH663_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ8174367.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRTQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIMENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(13950..15422)
                     /locus_tag="VH663_001163"
     CDS             complement(13950..15422)
                     /locus_tag="VH663_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8174368.1"
                     /translation="MLIVMENRNMTFLSIKNQETHLLTSTGKMLASHQAIKELLLNNQ
                     PNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPNPKNIGKPFQGGDEEAVLAGKKVI
                     STAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTTLNDVALTSKRNYTLSLLLCLLIS
                     LLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLDQIEAAVFVVDKAGILQLCNQAGQ
                     KLIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQLFRYGEEDYLLAISPICVKNDHR
                     GHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHKFMNQLHVIYGLVDIAYYDQLKIY
                     LDSILEPENEILTSLSVLVKEPLLASFLIGEQEKYQELNVHLKIDVLSEIPHSATKNQ
                     LNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHLISHYTLTDNWIDLERVQPIFDLP
                     YFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGGQHERFNH"
     gene            15384..16100
                     /locus_tag="VH663_001164"
                     /pseudo
     CDS             15384..16100
                     /locus_tag="VH663_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
     gene            complement(16182..17231)
                     /locus_tag="VH663_001165"
     CDS             complement(16182..17231)
                     /locus_tag="VH663_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEQ8174369.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTDAIVQLVKTTICGTDLHI
                     LGGDVPACKEGTILGHEGIGIVKEVGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSS
                     HCEDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVL
                     PSHVNPGDNVCIVGAGPVGLAALLTVQFFSPANIIMVDLSQNRLEAAKAFGATHTICS
                     GSSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFN
                     LDELWIKNITLNTGLVNANTTEMLLNVLKTGKIDATRLITHHFKLSEVEKAYETFKHA
                     GANNALKVIIDNDIS"
BASE COUNT         5258 a         2934 c         3680 g         5401 t
ORIGIN      
        1 aaatttcaat ggattactag cttatattta atagtcttgt ccaaatagga caattaaaca
       61 ttttttaatc aaatctcctt tatactgata ttaggagaat gttagatgta cccgcgtatt
      121 aggaatttga gagaagataa cgatttcact caaaagtttg ttgcaaattt actttccttc
      181 tctcatgcaa attatgcaaa aattgagaga ggagaggttg ccttaatggc agatgttcta
      241 aactctataa cgtcagtatt gattatttat tagggcaaac agattatccg tatcgttttc
      301 ataataaata aaagtctcgc cttgatttgt tcattgacaa ctgaataaac cgaggtggga
      361 ctttcttgtt tgtcgagcaa tttagtgaga tacgccgtga taggagcgat aattattagc
      421 tataaaatag agtggttctc tatctgccat gaccacaaat agggacaatg atacttctga
      481 acgttcaggc tgccagcgta aaaggccagt gggtgagatt cccatgagtg atttaaccag
      541 tcatacccac ggaggttgca atttttttga aaggagtgga ggtgtgaaaa tagtaacata
      601 taaaaagctt gatgaatatt ggttttctcg aacatactgc aagaaatatt attagagaag
      661 caaaaagaat agctataaaa aagtttgaag aagctcgcga aattgaccaa aatgcggtac
      721 aattaagtaa atcgcccttt gacaatagaa gacttggtat tgctcctaaa gatattgtag
      781 aagagttagt aggtattcta ctctctgaag aaagtttaga aagtgagaat taaagatgac
      841 tataaaaaat caaaaaaagt acaaaggtgt ttattgtgat aagaatggta agatatttta
      901 tcaagctgat cttggtgtag atccagtaac aggtaaacga gttcaaaaga aagccagaaa
      961 gaattagtat ggaaagccat ttggtacaat gaaagaagcg tatgatgaac tggttcggat
     1021 taggtatgag tttagtaata atcttagtat tgaaaatgac aatatatcgt ttgaaaacta
     1081 tatgaatact atttacctaa gagcatataa gcaaaaggtt caggctgtaa cttatgaaat
     1141 agccttacca catcataagt tatttattca gtattttggt tctaaacttt taaagggaat
     1201 aacagcttga gattgtgaat cttttagttt acatattatt gaaaattatg caaagaattt
     1261 atggtcgcga ttcaaagctt gcatggggta tgctgaaagg ttagactata tttcaaaagt
     1321 gccgtgtaag gctttggata atcctaaagg gaaacatccc gatactccat tttggactta
     1381 tattgagttt cagaatttta taaaatcgtt tgatttgcaa gattatgaag agctacaaag
     1441 atttacaact atttggctat attatatgac aggtgttcgt gtaagtgaag gtttatcatt
     1501 atgatgggaa gatatcgatt tcgaacaaaa atttttgaaa gttcatacaa ccttggaaaa
     1561 ggatgaaaat ggaaattggt atcgtaaaga ccaaacaaaa acgccagcag gagaacgtct
     1621 tattgagtta gatgatgtga caatagtagt attggaaaat tggagaagaa atcaagtagt
     1681 caatactgac acagatttca ttatttcacg gtttggtgaa ccattttgta aatcaacgat
     1741 ttgtcgagtt attaagcaca aagctcaaga agtaggagta ccagtgataa cgggaaacgt
     1801 cttgcgacat agtcatgctt cctatcttat taatgttttg aaaaaagaca ttctgtacgt
     1861 tgtaaagtgt atgggacatg ctgataagtc tacaactttg aatacatata gtcactggtt
     1921 taatgcatta gataaaacag tatctgaaga aataacccaa aatgtaataa gttcaggatt
     1981 agattccatt ttataccaaa attcctgcca aacaactaat tgatataacg aagcttatta
     2041 tatcgcccat ataacaggat gtgttattgt tacagggtac ttgctttatg agcttgttta
     2101 agctactaaa ggtgtgtgcc actatacttc cttccaaatt tctgagaatt tttgttataa
     2161 tagtaactat gaaagatata atgataaaaa ggtgggctat cctttttttt gtggtattaa
     2221 cttgttcagg gcttagtaaa atagtcctcg cagctgattt tcaagtggga gcagaacatg
     2281 cgattgtcgt agaggcagac tctgggagag ttctgtatga gaaagacgct aagacgccag
     2341 atgctattgc ctctttgacc aaattggtga cggcttatct ggttttagat aaggttaaat
     2401 caggccagct gcaattatca gatcgagtta atctctcaga ttatgccttc gagttaacaa
     2461 aagataggtc tttaagtaat gtaccttttg acaaaaaaac ttattcagtt caagacctgt
     2521 tgacagctac tttggtagca agctcaaata gcgctgctat tgcgttagcc gaaaaagtgg
     2581 cgggttcaga gcttcatttt gtaaatcaaa tgagagaaca gctatctcat tgggggatta
     2641 cttctggtaa aatcctcaac gcctcaggtc tgccaaatga ggtgttaaag gacaatcgtt
     2701 atcctggttc tgcgttggaa gaagaaaata ccttgagtgc tcaagatgtt gcagttgtga
     2761 cgatgcattt gttagaagat tttcctgaga ttttagagat caccaaacaa acagaagtta
     2821 attttgctgg caattctatt aaaagtttta atcaactctt accaggtatg gctaagggta
     2881 gagcaggagt ggatggttta aagactggga caacagactt ggcaggtcat tgtcttgtgg
     2941 taacttctat tgaaaatggt atgcgagtga ttaccgttat tctaaatgcc gatggatctg
     3001 ataaaaacca aaacacgcga tttgagcaag caaatcgttt gttggactat gtggatagaa
     3061 cttattgtcg tcgtaagatt cttaaaaaag gaagtcttgt ctcggagcga tcgctcccta
     3121 ttcaagatgg ccaagtaaaa gaactaccgg tatctgttgc cgaagatgta accatcattt
     3181 tgcaacaggg tgaacaagtt cccaaaccta aacaatttat gatatcagag acttctttac
     3241 tagcaccgat tactaaaggt gaagtggttg cttatttgac gtctccaaga ataacagatc
     3301 agtcagtgag gtatttaaag gaacctaagc gtattcctct taaagctagc caatctctta
     3361 aaaaagcctc tgacttacag ctgtggtggc gagattttct tgaaaaaaga cgttaaagat
     3421 aagaataaaa taattttaaa acaatcaaaa aagacagtta tgaaagaatt ttttcataac
     3481 tgtcttttta gtttacagtg agtatgattt ggaatgataa aaaaattgat gttatggttc
     3541 cattgtttgt aaaagatttt caggactcat gtttgggagg atacgaatcc ggttaagaga
     3601 aagaagtcca ttagccgcgc tagctattcc ttcattagtg gtaacaccta atttatagtt
     3661 aagtctagct gcaatttgta aggtagtgtc attatatcta ccagatggat aagcaatggc
     3721 aatagtgttt tggtttaatt gcttatcaag atagtccttt gaatctttca tttcagttgt
     3781 ttgaacatca ggactagcct gctctaaatc aggatgattc actgtatgat cttggaaaga
     3841 cataccgaat tgtttcattt ctttcatctg cttaagagtc agattggcag cgcttcctat
     3901 ttcggttagt cctgtaataa cattattggt ggctttagca tcatattttt ttaagattgg
     3961 gtaagcaacg ttgtagaaat caatcatact gtcatcaaat gttagccata caactttttt
     4021 agcaggcaat tcattatttg aaagagcgcg gtagacctct tcaggactca aaaaatagta
     4081 gccctcgtct ttcatttttt gaagttgttg atcaaagaga tctgggttga caatcaaatt
     4141 agcattggcc gtctcttcag gggacattac atgaatagca tgatacatta aaattgggat
     4201 cttgacaggt gtttcttgct tggtccaagt taatttagaa tgggcctttt tttgagattt
     4261 tgaggaatcg aaatgagtct tcttaagttt tgtcgtttct ttttttgccg tctttttgtg
     4321 atgaaaaaga cttattttag gtacaaattg agagacatct gacaagccga atttgttaga
     4381 cccttgatat aaatagaaaa tgagggtcaa accaagaatg caggcaaata atagtataag
     4441 gttaatgaga gtgaaggctt ttcttttttg gcgccgttta tgtctattgt tcattacttg
     4501 ctcctttttt tgatataagt acctctattg taacattttt tagagaaaag aaggcttgaa
     4561 gtcagccttt tttcttgttt tttggaagaa cgtgtattaa ggagtaaaaa aattgttgtc
     4621 acagataaca ctttaggagg tagcaaagac tatgtgggac tggtattctt atcacgtaaa
     4681 aaaagcaatg aagataaaag tgtagtgtac tttagaactt atttgagtgt ttggtgagga
     4741 taagaaaaaa aaagggtact actctaggtt atctgctgtc actttttttt atatatggta
     4801 tactaaagat agatgtgagg tatgaaatga cttatgaaga gactctagaa tggattcatg
     4861 atcacttagt tttcggcatc aagccaggct taaaacgtat gctttgggtt cttggacagt
     4921 taggaaatcc ccaaaaaaat gtcaagggag tgcatatcgt tggaactaac ggaaaaggtt
     4981 ccacggtcaa tcatttacaa cacatcttta cgacagctgg ttatgaagtg gggaccttta
     5041 cctctcctta tattatggat ttcaaagaac gtattagcat aaatggtcga atgatttcag
     5101 aaaaagattt ggttattgct gctaatcgta ttcgtccttt gacagagcgt ctagttcaag
     5161 agactgattt tggagaggta actgaatttg aagtgattac tttgatcatg ttcctctatt
     5221 ttggtgatat gcatcctgta gatattgcta ttattgaagc aggccttggc ggtctttatg
     5281 attcgaccaa tgtttttcaa gcaatggttg tggtctgtcc ctcaattggt ttagatcacc
     5341 aagctatttt gggagagact tacgctgata ttgccgccca aaaagctggt gttttagaag
     5401 ggggcgagac ccttgttttt gccgttgaaa acccgtcagc tcgagaggtt tttttaacaa
     5461 aagctgagca ggttggtgct tcaatctggg aatggcaaga gcagtttcaa atggctgaga
     5521 acgcatctgg ctatcgtttt actagtcctt taggagttat ttcagatatt cacatcgcta
     5581 tgccaggaca ccaccaagtg tctaatgcag ctttagctat tatgacttgc ttaaccttgc
     5641 aagaccgcta tcctaggcta actccagatc atataaggga aggtctggca aatagtttgt
     5701 ggctaggccg taccgaatta ttagcaccaa atctaatgat tgatggagct cataacaatg
     5761 aaagcgttgc tgctttggta gccgtcttaa aaaataacta caatgataaa aagcttcata
     5821 ttttgtttgg tgcgatcgac accaagccta ttgtagatat gttggttgct cttgagcaaa
     5881 taggtgactt gcaggttact agctttcatt accctaacgc ctatccattg gaaaaatacc
     5941 cagaacgttt tggtagggtt gctgatttca aagatttctt ggccttgcgt aagcatgcta
     6001 aagcagatga ctttttcgtg attacagggt cactatattt tatttcagaa attagacggt
     6061 attggaaaaa acatattgaa aaaagcgttc ttttaaccca ttaaaaaaga gaaaatgaaa
     6121 aggaaaagga aaaaactaat gaaaagggaa agactaatgt ctattaataa ggaaaaagca
     6181 gaagcagcca tttatcagtt tttagaggct attggtgaaa atccaaatcg agaaggtctt
     6241 cttgacacgc ctaaacgggt tgctaaaatg tatgcggaga tgtttttggg actgggaaaa
     6301 gatcctaaag aggaatttac ggctgtcttt aaagaacacc atgaggatgt ggtgattgtc
     6361 aaagacatta gtttttattc tatatgtgaa caccacttag ttccttttta tggcaaggct
     6421 catatcgctt atttaccaag tgatggtcga gtaacaggtt tgagtaaatt agcgcgagca
     6481 gttgaagtag ctagtaaaca acctcaactc caagagcgtt tgacctctca aatagcggat
     6541 gctttagttg aagccctcaa tcctaaaggg actttagtta tggtagaagc agagcacatg
     6601 tgcatgacca tgcgaggcat caaaaagcca ggtagtaaaa ccattaccac cactgccaga
     6661 ggcttataca aggaaagtcg tgctgagaga caagaagtta tttctctaat gacaaaagat
     6721 taggagaact tatgaaaatt ggaaagtttg tgattgaggg caatgcggct atcatgggga
     6781 ttttaaatgt gactccagat tctttttcag atggggggtc ttacactact gtgcaaaaag
     6841 cattagatca tgttgagcaa atgattgctg atggggctaa aatcatcgac gttggtggag
     6901 aatcagcacg tccaggttgc caatttgtaa gcgctaccga tgaaattgac cgggtggttc
     6961 ctgtgatcaa ggccatcaaa gaaaactatg atattctaat cagcattgat acctataaaa
     7021 ccgaaacagc tagagcagct ttagaggcgg gtgccgatat tctcaatgat gtttgggcag
     7081 gtttgtacga cggtcaaatg tttgccttag cagccgagta cgatgcgcct atcatcttga
     7141 tgcataacca agacgaagaa gtttatcaag aggtaacaca agacgtttgt gattttctag
     7201 gcaatagagc acaagcagct cttgatgctg gcgtgccaaa aaacaatatt tggattgatc
     7261 caggatttgg atttgccaaa tctgttcaac agaatacgga gttattaaaa ggattggacc
     7321 gcgtctgtca gttgggctat cctgtcttgt ttggtatttc gagaaagcgt gtcgtagatg
     7381 ccttgttagg cggcaacacc aaagctaaag agcgagacgg agcgacagca gccttgtctg
     7441 cttatgccct tggaaaaggc tgtcagattg tacgcgtaca cgatgtcaag gctaatcaag
     7501 acattgtggc tgtgttgagc cagttgatgt gaggacttat ggataaaatc gtattagaag
     7561 gttgccgttt ttatggctac catggagcct ttaaagaaga acagaccctt gggcaaattt
     7621 ttctggttga tttagaattg tcagtcgatt tgcaagcagc ttctttgtca gaccaattaa
     7681 cagatacggt ccattacggg atggtgtttg atagtgtccg tcagttagtg gaagggggaa
     7741 agtttatttt gattgaacgt ttagcaggtg cgatttgtga acagcttttt aacgaatttc
     7801 cacctattga agccatcaaa gtggccatta aaaaggaaaa cccaccgatt gcaggccact
     7861 ataaggcagt tggtattgaa ttggagagac agagatgacc attgtttatt taagtttagg
     7921 caccaatatg ggggaccgag cagcttactt gcaaaaagct cttgaggctt tagctgactt
     7981 acctcaaaca cggttgcttg ctcaatcatc catttatgaa acgactgctt ggggcaagac
     8041 aggccaggct gattttctca atatggcctg tcaattagat acacaattaa ccgcggctga
     8101 tttcttaaaa gaaacacaag ctattgagca atctcttggt cgtgtgaggc atgaaaaatg
     8161 gggatcaaga actattgaca ttgatatctt gctatttgga gaagaggttt atgacacaaa
     8221 ggaattgaaa gtgccacacc cttatatgac tgagcgtgct tttgttttaa tccccttatt
     8281 ggaattgcag ccagatttaa aattgcctcc taatcataag cttttaaggg attatcttgc
     8341 agctttagat caatcagata tcacgctctt ttcagctcaa cagacagagt tctagactct
     8401 gttttcggag taatgcctgc tataagccaa tccataaagt tttgccaaat tcatcgttat
     8461 tttttctaaa atttgatata atagtatcgg ctttatgccg atttttttac gcgtataaga
     8521 agtgataaaa gaaagaagat aactatgata actgaacttc atgggattga tattcgagag
     8581 aatgaacccc taaaacatta cacttacact aaggtaggtg gcccagcaga tttcttggct
     8641 tttcctagaa atcactacga gctatcacgc atcgttgttt atgccaacaa agaaaatatg
     8701 ccttggcttg ttttgggaaa tgccagcaat ctcattgtgc gagatggtgg tattcgtggc
     8761 tttgtcatca tgtttgataa gctaaatgca gtgcacttaa atggttatac cttagaagcc
     8821 gaagctggtg ccaatctgat tgaaacaacg aagattgcca aattccatag tttaacaggg
     8881 tttgaatttg catgtggcat tcctggaagc gttgggggtg ctgtttttat gaatgcgggt
     8941 gcctatggtg gggaaatatc acatattttc ttatccgcta aggtgcttac ctcaagtgga
     9001 gagatcaaaa ccatttcagc tagggatatg gcttttggtt accgtcactc tgccattcaa
     9061 gaaacaggtg acattgtcat ttctgctaag tttgctctta aaccgggtaa ttatgatacg
     9121 atcagccaag aaatgaatcg gttaaatcac cttcgccaac taaaacaacc tttagaattt
     9181 ccatcttgtg gatctgtgtt taagcgcccg ccaggacact ttgcaggcca attaatcatg
     9241 gaagcaaatc ttaaagggca tcggattggt ggtgtggaag tttccgaaaa acacgctggt
     9301 tttatgatta atgtggcaga tggcacagct aaagattatg aagatttgat tgcttatgtc
     9361 attgagacag ttgaaaacca ttctggtgtc aggcttgaac cagaagttcg gattattggg
     9421 gaaaacctgt aaccattatt gataattaaa gaaagtaccg tggaggattt atgacaattg
     9481 actaagccaa ttatcacatt taacaatgtt tcaaaaacat ttgaggacag tggaacacag
     9541 gttctaaaga acattaactt tgaccttgaa gaaggaaaat tttacaccct gcttggagct
     9601 tctggctctg gaaaatcaac tattttaaat attatggcgg gcctactaga tgccagcagc
     9661 ggagacattt atttagatgg ggaacgtatt aatgatttgc cgattaataa acgtgacatt
     9721 cataccgttt tccaaaatta tgccctcttc cctcatatga ctgtttttga gaatgttgcc
     9781 tttgctttga agctgaagaa agtggacaag aaagagattg ctaagcgcgt gaaagaaacc
     9841 ttgaaaatgg ttcaattaga agggtatgaa aatcgttcta tccagaagtt atctggtggt
     9901 caacgtcaac gtgttgccat tgcgcgtgcg attatcaatc aaccgcgtgt ggtcttactt
     9961 gacgaaccac tctcagccct tgatttgaaa ttaagaacag agatgcaata tgaattacgt
    10021 gaattgcaac aacgtctagg cattaccttt gtttttgtta ctcacgatca agaagaagcc
    10081 ttggccatga gtgattggat ttttgtcatg aatgaaggtg aaattgttca gtcgggaaca
    10141 ccagtggata tttacgatga gccaattaat cattttgttg ctaattttat tggagaatct
    10201 aatattatta acggtaccat gattgaagac tatcttgtct cctttaacgg gaaagaattt
    10261 gaatctgtgg acggtggaat gcgccctaat gagcctgttg aagtggttat tcgtcctgaa
    10321 gatcttcaaa ttactttgcc agaagaaggg aaattacaag ttaaggttga tacccaatta
    10381 ttccgcgggg ttcactacga aattattgcc tatgatgaat tgggtaatga atggatgatt
    10441 cattctaccc gcaaagctat cgagggagaa gttatcggat tagactttac ccctgaagat
    10501 cttcatatca tgcgtcttaa tgagactgaa gaggaatttg atgcccgtat tgaagaatat
    10561 gtggaaatgg atgagcctga agatggattg attaatgcca ttgaggagga gcgtaatgaa
    10621 gaaaacctct agtctttttt cgattcctta cttcttatgg attctctttt ttgttgtggc
    10681 accagtcact ctcttgtttt acaagtcctt ttttgacata gaagggcgcg tgaccttagc
    10741 caattatgaa acctttttta gctcttggac ctatttgaga atgagtgtga attctatttt
    10801 atacgctggt attatcacac tcgtcacgct cttaatttca tatcctacgg ctctcttttt
    10861 aacgcgccta aagcacaagc agttgtggct tatgctcatt attttgccaa cttgggtaaa
    10921 cttattgcta aaagcctatg cctttatggg aatctttggt caacaaggag ggattaacag
    10981 ctttttaact tttatgggga ttggcccgca gcaaatcctt ttcacggatt tctccttcat
    11041 ttttgtagcc tcttacattg agcttccttt tatgatgtta ccgattttta atgctttgga
    11101 tgatattgac cataatgtca tcaatgccag tcgcgatcta ggagctagtg aatttcaggc
    11161 cttctcaaaa gttatttttc ccctttcttt aaatggggtt agggcaggtg ttcagtctgt
    11221 ctttatccca agtttgagtc tctttatgtt aacccgtttg attggtggaa accgcgtgat
    11281 tacacttggt acagccattg aacaacattt tttgaccacc caaaactggg gaatgggatc
    11341 aaccattggt gtcgtcttga ttttgaccat ggttgctatt atgtggctca caaaggagaa
    11401 aagtaaatga aaaaatttgc caatctttat ttagcgagtg tctttgtttt actctacatt
    11461 cctatttttt atttgatttt ctattctttc aacaaaggtg gggatatgaa tggttttaca
    11521 ggatttaccc ttgagcatta ccaaaccatg tttgaggata gtcgtctcat gacaatctta
    11581 ctgcaaacct ttgtccttgc ttttagtagc gctctactag caacgattat tgggaccttt
    11641 ggagctatct ttatccacca tgttagaggt aagtaccaaa atgccatgct atcagccaat
    11701 aatgtcttga tggtatcacc agatgtcatg attggggctt cctttttaat tctttttaca
    11761 tcattgaagt ttcagctggg catgtcttca gttttattaa gtcatattgc tttttcgatt
    11821 cctattgtgg ttttgatggt gttgccgcgc ttgaaagaga tgaatcaaga catggtcaac
    11881 gccgcttatg atttgggagc taattatttc caaatgctca aagaagtcat gctgccatac
    11941 ttaacaccag ggattattgc aggttatttt atggccttta cctattcctt agatgatttt
    12001 gcagtgactt tctttttgac tggaaatggt tttactactt tatctgttga gatttattcg
    12061 cgggctcgtc agggaatttc cttggatatc aatgctttgt caaccatcgt tttctttttc
    12121 tccatcctct tagtgatcgg ttattattat atgtcacagg acaaggagga aaaacatgcg
    12181 taaactttat tcctttctag caggagtttt gggtgttatt gttattttaa caagtctttc
    12241 tttcatcttg cagaaaaaat cgggttctgg tagtcaatcg gataaattag ttatttataa
    12301 ctggggagat tacattgatc cagctttgct caaaaaattc accaaagaaa cgggcattga
    12361 agtgcagtat gaaactttcg attccaatga agccatgtac actaaaatca agcagggcgg
    12421 aaccacttac gacattgctg ttcctagtga ttacaccatt gataaaatga tcaaagaaaa
    12481 cctactcaat aagcttgata agtcaaaatt agttggcatg gataatatcg ggaaagaatt
    12541 tttagggaaa agctttgacc cacaaaacga ctattctttg ccttatttct ggggaaccgt
    12601 tgggattgtt tataatgatc aattagttga taaggtgcct atgcactggg aagatctgtg
    12661 gcgcccagaa tataaaaata gtattatgct gattgatgga gcgcgtgaaa tgctaggggt
    12721 tggtttaaca acttttggtt atagtgtgaa ttctaaaaat ctagagcagt tgcaggcagc
    12781 cgagagaaaa ctgcaacagt tgacgccgaa tgttaaagcc attgtagcag atgagatgaa
    12841 aggctacatg attcaaggtg acgctgctat tggaattacc ttttctggtg aagccagtga
    12901 gatgttagat agtaacgaac accttcacta catcgtgcct tcagaagggt ctaacctttg
    12961 gtttgataat ttggtactac caaaaaccat gaaacatgaa aaagaagctt atgctttttt
    13021 gaactttatc aatcgtcctg aaaatgctgc gcaaaatgct gcatatattg gttatgcgac
    13081 accaaataaa aaagccaagg ccttacttcc agatgagata aaaaatgatc ctgcttttta
    13141 tccaacagat ggcattatca aaaaattgga agtttatgat aatttagggt caagatggtt
    13201 ggggatttat aatgatttat acctccaatt taaaatgtat cgcaaataag cactcaaaaa
    13261 ggctggtatt tctccagcct ttttaatgta tcgacagatg acgtcaccag tacacgcgat
    13321 agggacggcc aaccttggta aaaatttgtt ggctgttcag ttgcttattt tcttctaaat
    13381 aagcaatgta tttacggaca gagacatggg ataagtgaca agcggaagct aattcttgga
    13441 tagtaaaagg ttgatcaaag gctttgatat tttccataat ccactggaaa gtggattcag
    13501 ataagccttt ttctaacaat tgtttgtttt tagtgtcttt tttgctagtc aaacatttca
    13561 gttggtcaat ttgggcctgc tctagttgct gattggccaa atgttctcga tgtgtaacaa
    13621 actgttggat gctttcttga aagcgttcaa aggtaaaggg cttgatgagg tagtcgataa
    13681 ttcctaaatg aaaaccatct cggataatat tcccatcatt tgcggctgaa ataataatga
    13741 cttcgcaagg aatatgctga gtacgccatt tctccaaaaa ttgaatccca ttgccatctg
    13801 taatatgaat atccagtaaa atgaggtcaa ttgcgtaatc agtaagtatg ctttgaacag
    13861 ctttcataga gtcgctggat ataatgcgat caaaaagatt tagtttttcg aggtaattgc
    13921 gatggataaa atccaccata ggatcatctt caatgattaa aacgttcatg ttgccctcct
    13981 acataaggtg ttgtcactga gaactgtaac tcggttacgg tttgatgttg atggaattgg
    14041 gaatttgtat cggtcaagag ctgttgaaaa taaggtaagt caaaaatagg ctgtacccgc
    14101 tccaaatcaa tccaattgtc tgtcaaggtg taatggctga ttaaatgatt ctgatcatgt
    14161 tgaatactca aaacgagtga tttgggtctc agtgtggtta gaagattagt atggataaag
    14221 cggtaaatca tcaaaccatt attaagttga ttcttggtgg cagaatgagg aatctcgctt
    14281 aaaacatcaa tcttgagatg gacatttaat tcttggtatt tctcttgctc tccaatgaga
    14341 aaactagcta gaagaggctc cttaacaagg acagataagc tagtgagaat ttcattttcg
    14401 ggctccaaaa tactgtctaa ataaattttc aattggtcat aataagcaat atccactaag
    14461 ccataaataa catgcaattg attcatgaat ttatgagttt gggcttgtag agcagaagca
    14521 taggcagtgg tataggctag ctgatccaag gtatcaatag ccttaactgc ttctcgcatg
    14581 aaaataatgt ggcctcggtg atcgtttttg acacagatgg gtgaaatggc taacaagtag
    14641 tcttcctcac cataccgaaa gagttgttca tgcccctctt gcaaactaag ttttggaaaa
    14701 tcaggaaaga ggtgattgaa actgttgcca gtaggttttc ccaactggca tttccgagca
    14761 attaattttt gtccagcctg attgcagagc tgtaagatac cagctttatc taccacaaat
    14821 acggcggctt cgatttgatc caacatggca ttacgctctt caaagagctg gtaaatttca
    14881 cttggttcta actggtgcag ttgacgcttg agtcgaaaag aaataaaact ggtgaccagt
    14941 aaactaataa gaagacaaag gagtaaagac agggtataat tgcgttttga cgttaaagcc
    15001 acatcattta gggtagttag tttaatgcca accgcaatag caccaatctg tttgtcaccg
    15061 tcaaacacag gtactagata gcgaagtgac tttcctaaag tgcccttggc ggtagagata
    15121 accttttttc ctgctagaac agcctcctca tcacctcctt gaaaaggttt tccgatattt
    15181 ttcgggttag gatgggtgag tcgaatccct ttcatgttca tcacgaccac ataatcaagg
    15241 ttatagatgg aggcaatgga gttggtatag gctgttgttt tagcatttgg ttggttgttt
    15301 aggagtaatt ctttaattgc ttgatgactt gctaacattt ttccagttga cgttagcaaa
    15361 tgggtttcct gatttttgat ggaaaggaat gtcatattcc tgttttccat gacgatcagc
    15421 atggaacagc tattgtggtg ttagcagcca tctttaatag tttgaaactt cttcaaaaat
    15481 cccttgatga ggtatccatc gtggtgaacg gtggtggatc tgcgggatta tccattaccc
    15541 gtaaattatt agcagcaggt gctacgaaag tgacagtagt ggataaattt ggcattatca
    15601 atgaacaaga ggcagatcag ttagcaccac accatttgga tatagcgaaa gtcactaatc
    15661 gtgagtttaa atcaggtacc ttagaagatg ctcttgaagg agcagatatc ttcattggcg
    15721 tatcagtacc aggagtctta aaagcagagt ggattagtaa aatggcagcg cgcccagtaa
    15781 tttttgccat ggccaaccct attccagaga tttaccctga tgaagccctt gaagcggggg
    15841 cttacattgt aggtactggc cgtagtgatt ttcctaacca gattaataat gtccttgcct
    15901 ttccaggtat ttttagggga gcattagatg cacgggcaaa aaccattact gttgagatgc
    15961 aaattgctgc cgcaaaaggt attgccagtc ttgttccagg tgacgctttg agcactacta
    16021 atattattcc agatgccttt aaagaaggag ttgctgagat tgtcgctaaa agcgtcaggt
    16081 cggtagtcct taaatcatag aatatatgaa aaaaagagaa agttatactt taaatcacaa
    16141 aataagtaga agcatatttc tagcttctac ttattttgtg attagctaat atcattatca
    16201 ataataactt tcaaggcgtt atttgcacca gcatgtttaa aggtttcata agctttttca
    16261 acttctgaaa gtttaaaatg atgtgtaata agtcttgttg cgtcaatctt tccagttttc
    16321 aagacattta gtagcatttc agttgtattg gcattaacaa gtccagtgtt taaggtgata
    16381 tttttaatcc ataattcatc caggttgaaa tcaactggtt tcccatgaac cccaacgtta
    16441 gcaatatgcc cacctactga tataattttt tggcaaatat caaatgtcgc gggataacca
    16501 acacactcca tggaaatatc gacaccacgt ccattggtta tatcgtcaat gatagccttg
    16561 acttcttcac tacttcctga acaaatcgtg tgtgtggctc caaacgcttt agctgcttca
    16621 aggcgatttt gagataagtc gaccataata atattggctg gagagaaaaa ttgaacggtt
    16681 agtagggctg cgagtcctac tggaccggca ccaacaatac aaacattatc tccaggattg
    16741 acatgtgatg gcaagacacc aatttcatat gaagttggta aaatatcaga aagcatgaca
    16801 agtgcctcat catcaatcgt atcaggagca tgatagagac taccatctgc atgtgggata
    16861 tggacatatt ccgcttgtgt tccattaatt aaatggccaa gaatccaacc gccatcttca
    16921 caatgtgacg aaaggcctcg tttacaataa taacaggtat gacatgatgt gacgcaagaa
    16981 ataatcactt tatctccgat tttaaagtta gtaaccgcat caccgacctc cttaacaatt
    17041 ccaattcctt catgaccaag gattgttcct tctttacatg cgggaacatc cccacctaag
    17101 atatgcaaat cagttccaca aatagtggtt tttaccaact ggacgatagc atctgtaggc
    17161 tttataatga ctggttttgg tttgtctatg agctgtagat ttccggttga aaggtaagtt
    17221 gctgctttca tatttaactc cttataaggt tgagaggtaa tcaaccaatt cat
//
LOCUS       DAUGFP010000033        16447 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.033, whole genome shotgun sequence.
ACCESSION   DAUGFP010000033 DAUGFP010000000
VERSION     DAUGFP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16447
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.033"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..540
                     /locus_tag="VH663_001166"
     CDS             <1..540
                     /locus_tag="VH663_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEQ8174370.1"
                     /translation="VIRYSDRYIFTNGGTLRIDLKDFFSGAHSDPRNYLIQEIFRFLN
                     LCEKAGTGIPKIMEAVKESRLKYPKLRTQLDSVELTLWDTSLIDNLYIDNEYEKKILE
                     LIIENRFITRETLEEKLKVHKNTILKYLKKLVEKQAIDKFKSGRQYVYFIAQNQDPEF
                     QKYNHIDAMYSLLEEMKRK"
     gene            635..1531
                     /locus_tag="VH663_001167"
                     /pseudo
     CDS             635..1531
                     /locus_tag="VH663_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481078.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1626..1862
                     /locus_tag="VH663_001168"
     CDS             1626..1862
                     /locus_tag="VH663_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEQ8174371.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGAGEGFSMWAPTPCDVLAEDWIEVND"
     gene            1855..2553
                     /locus_tag="VH663_001169"
     CDS             1855..2553
                     /locus_tag="VH663_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEQ8174372.1"
                     /translation="MTKVVLVTGCASGIGYAQARYFLKQGYHVYGVDKSDKPDLNGNF
                     HFIKLDLSSELSPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLAEWVARETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWTLK"
     gene            2629..3588
                     /locus_tag="VH663_001170"
     CDS             2629..3588
                     /locus_tag="VH663_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ8174373.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYHMPLVTSKYEAILGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIAKKRKLILLEAITNQYLSNMTFIKEHLDQLGDIKIVECNYSQYSSRYDAF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKTTIQGNKGSLAVLGATNTLPQVQLSLYGHEAQVVNHNKH
                     DHRMYEEFVAFKDMIDQKDFEKVNQALEHSRAVMAVLERAVHS"
     gene            3921..5258
                     /locus_tag="VH663_001171"
     CDS             3921..5258
                     /locus_tag="VH663_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ8174374.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGILVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISILEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            5431..6813
                     /gene="glmU"
                     /locus_tag="VH663_001172"
     CDS             5431..6813
                     /gene="glmU"
                     /locus_tag="VH663_001172"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEQ8174375.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKSVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGQGSIITNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDNALTAAGSTISKTVPADSIVIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            6844..7398
                     /locus_tag="VH663_001173"
     CDS             6844..7398
                     /locus_tag="VH663_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ8174376.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKIVLVKQYRKAIESVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQIANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            7398..7649
                     /locus_tag="VH663_001174"
     CDS             7398..7649
                     /locus_tag="VH663_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174377.1"
                     /translation="MGRPLLTDDIIEKARRMETFESDDAVDFDTKVMTLPEKDEKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            7669..8364
                     /locus_tag="VH663_001175"
     CDS             7669..8364
                     /locus_tag="VH663_001175"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEQ8174378.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQTLMTFLENLPV"
     gene            8515..8856
                     /locus_tag="VH663_001176"
     CDS             8515..8856
                     /locus_tag="VH663_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174379.1"
                     /translation="MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGGYRQGLTD
                     EKSKLQYSKDNAKLQLRNILNGYQSDLGKHYSSYYYYNLRTVMGLSSEQDIEEHYKKL
                     KNKLHDMYNHY"
     gene            complement(8955..9602)
                     /locus_tag="VH663_001177"
     CDS             complement(8955..9602)
                     /locus_tag="VH663_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEQ8174380.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILNFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLEAHHLNINTELTLTQIDTFAKTYTICYDDKDLVIPENIAKQLYVTAL"
     gene            9748..10680
                     /locus_tag="VH663_001178"
     CDS             9748..10680
                     /locus_tag="VH663_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEQ8174381.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            10744..11469
                     /locus_tag="VH663_001179"
     CDS             10744..11469
                     /locus_tag="VH663_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174382.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            11470..12324
                     /locus_tag="VH663_001180"
     CDS             11470..12324
                     /locus_tag="VH663_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ8174383.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLGVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(12472..13278)
                     /locus_tag="VH663_001181"
     CDS             complement(12472..13278)
                     /locus_tag="VH663_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEQ8174384.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPEQDITITSIDIVKDYRFKN"
     gene            13495..15900
                     /locus_tag="VH663_001182"
     CDS             13495..15900
                     /locus_tag="VH663_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ8174385.1"
                     /translation="MVKRNQRKKSAPKKRLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQDIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMPKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVEVDFTPKTHLLYKLPTI
                     DLFAPDRPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKIGVRNIA
                     GYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRMAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGSAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(15970..16323)
                     /locus_tag="VH663_001183"
     CDS             complement(15970..16323)
                     /locus_tag="VH663_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEQ8174386.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTSLFKLNKKGFKFPDIALLLFA
                     VEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
BASE COUNT         5057 a         2794 c         3592 g         5004 t
ORIGIN      
        1 gtaattagat attctgatag atatatattt acaaatggcg ggacacttag aatcgaccta
       61 aaggactttt ttagtggggc acattctgat ccaagaaact acttaataca agaaattttt
      121 aggtttctaa atttatgtga aaaagcggga actggtatac ctaaaataat ggaagcagtt
      181 aaagagagtc gtttaaaata tcccaaatta cgaactcaat tagactcagt tgaacttaca
      241 ttatgggata cttcccttat agataaccta tatattgata atgaatatga gaaaaaaata
      301 ttagagttaa taatcgaaaa ccgattcata acaagagaga ctttggaaga aaaactaaaa
      361 gtccataaaa atacaatttt aaaatattta aaaaaattag tggaaaaaca agctatcgat
      421 aaattcaaat ctggtagaca atacgtatac tttattgcac aaaaccaaga tcctgaattt
      481 caaaaatata accacattga tgccatgtat tctctactgg aagaaatgaa acgcaaataa
      541 tggtcttctc gtaagcttaa gcatgatata aatccaatct aacaccgttt tgataggagc
      601 ttgcctgctg atgctgatgg aaaggtttat gatcatgaaa ataacagaag ttaaaaagaa
      661 agatggtgct attgtctacc gtgctagtgt ttgccttggt attgatcagg taacaggtaa
      721 gaaagtaaaa atcagtgtaa cgggtaagac gcaaacagag gttaagcaga aagccaaaca
      781 cgcgcagttt gattttatat ttaatggatc tacaatatgt aaagaagcac agattaggaa
      841 ttatcaggag ttggcagaac tatggctaaa gagctatcag ctcactgtta agccacagac
      901 atatgtagcg actaagagaa tgctttacaa tcatttaata cctgtttttg gatctatgaa
      961 aattgataaa ttaactgtca gctatatcca acactttata aatgacttat caagtcagtc
     1021 agaccattac ggtgtagtgc attcgacaaa tagacgtgtt ttacagtatg gtgtatctct
     1081 ccaacttatt ccaaagaact acagtaggtt attaaaacta ttaggtacac caaagagtaa
     1141 agcaagaatt agggcaatca gtatcgacaa gaaaacagtt aatatgttaa agctttacaa
     1201 gaagagacag cgacaactgt ttttagaagt cggcggttgt tctcctaagg tggtttttgc
     1261 tactccaacc aaagaatatc aaaacatggc gacaagccag gagtgtttaa acagatgatg
     1321 caaggaaata gatattcctc gctttacctc tcacgctttt agacatactc atgctagttt
     1381 attggtaaat gctggtatca gctataaaga attacaatac agatcaggtc atgccacttt
     1441 ggctatgact atggatagtt atagtcactt atcaaaagac aaagaaaaag aaccagtatc
     1501 ttattatgaa aaagcaataa atagcttcta ggtgaactaa aaggcgacca aatgtttaaa
     1561 attacatttt ataacattca caaaatctat tatatcaacg gatatagtca ataaaggaga
     1621 acaatatgac atttgaagaa attctaccag gtttaaaagc caagaaaaaa tatgtacgta
     1681 caggttgggg tggcgctgaa aattatgtgc agttgtttga tacccttgaa gtggatggga
     1741 aagtactaca ggcgacgcct tattttctga ttcatgttac tggtgcaggt gaaggttttt
     1801 ccatgtgggc accgacacca tgtgatgtgt tagcagagga ttggatcgag gtcaatgact
     1861 aaagttgttt tagtgacggg ctgtgctagt gggattggtt atgcacaggc ccgctatttt
     1921 ttgaaacaag ggtatcacgt atatggtgtg gataagtcgg ataagccgga cttaaacggt
     1981 aattttcact ttatcaaatt ggatttatcc tctgagttat ccccactatt taaagtggtc
     2041 cccagtgttg atattttgtg taataccgca gggattttag atgcctacaa gccactttta
     2101 gacgtcagtg atgaagaagt ggaacaccta tttgatatta atttttttgc gacggtgaag
     2161 ttaacacgtc attatcttag acgtatggtg gaaaagcagt caggtgttat catcaatatg
     2221 tgctcaatag caagttttat agcgggtggc ggtggtgttg cctatacgag ttctaagcat
     2281 gcccttgcgg gattcacgcg ccagctggct ttggattacg ccaaagatca gattcacatc
     2341 tttggtatag cgccaggtgc tgtcaaaact gctatgacgg ctagcgactt tgagcctggt
     2401 ggcttagcag aatgggtagc tcgtgagacg ccaattgggc gttggactaa accaagtgaa
     2461 gttgctgaat tgacgggatt tcttgcttca ggaaaagcga gatcaatgca aggtgaaatt
     2521 gtcaaaattg acggtggctg gactttgaaa tgatcacatg ccttagcttg agctggcgca
     2581 gtaagcccta gaggtgtgac gagtcaaaca gtcaaaggag atgaacaaat gaaattagcg
     2641 gttttgggga caggtatgat tgttaaggaa gttttgcctg tcttgcagaa aattgatggt
     2701 attgatttag ttgctatctt atcaacagtt agaagtttaa agacagctaa agacctcgct
     2761 aaggcatatc acatgcctct agttacgagt aaatatgagg ctatcttagg caatgaggag
     2821 attgacacag tttatattgg attgccgaat cacctgcatt ttgcctatgc taaggaggct
     2881 cttttggctg gtaaacatgt catttgtgaa aagcccttta ccatgacagc aggagaatta
     2941 gatgaactgg tggtgatcgc taaaaaacga aaactcattt tgttagaagc gattacgaat
     3001 cagtatttaa gtaacatgac ttttatcaaa gagcatcttg accaacttgg cgatatcaaa
     3061 attgtagaat gcaattattc acaatattct tcacgctacg atgccttcaa acgaggtgat
     3121 atcgcgccag cttttaatcc taaaatgggt ggtggggctt tacgtgattt aaacatctac
     3181 aacattcatt ttgtggtagg attatttggt cgccctaaaa cggttcaata tttagccaat
     3241 gtcgaaaaag gcattgacac atcaggcatg cttgtcatgg actatgagca gtttaaagtg
     3301 gtgtgtattg gggccaaaga ctgcactgcg gaaatcaaaa caaccatcca aggtaataaa
     3361 gggtcactgg cagtgcttgg agcaacaaat acccttcctc aggttcagtt aagtctgtac
     3421 ggtcacgaag cacaagtggt taatcacaac aaacatgacc accggatgta tgaggagttt
     3481 gtcgctttta aagacatgat tgaccaaaaa gactttgaga aagtgaatca ggctttggag
     3541 catagccgag ctgtcatggc tgtgttggag cgtgctgttc attcgtgact gtaatctggt
     3601 agccattgcc ttttgtgcag ttgctatggt aatggttgaa agacgtgttt tcacgagggt
     3661 taaccccctc gtgctttttt ggcaaaaata acttgttcag gatttgggcc ttctttgtga
     3721 gatgagagat gcctgacgaa ttgatttgat taaaataatg ataaagccaa acgatttaag
     3781 aaatgaccgt tcggaaattc tgccccaaaa ggtctctaga agcagtgaaa tcgaatttgt
     3841 aagcgttgac actactgcat aactctgtta cactaagaga atgtcatgac aaaaattaca
     3901 gtgtaggagt ttaattgttt atggaagagg attataacaa aagagaacct gagaagttta
     3961 cgcaatttct gcgccgtcaa aaggttgtct tttttgtcgc attttttggt tatgtgtgtg
     4021 cttacttggt acgcaacaat ttcaaactaa tgtcaaacac tattatggtg caaaatggtt
     4081 gggacaaggc tcaaattgcg accttgttat cgtgtttgac cgtttcttat ggcttagcaa
     4141 aattctatat gggagcctta ggggatcgtg ttagcttgag aaagctcttt tcgatcagct
     4201 tgggtgcaag cgctttgatt tgtattttaa tcggtttttt caatagttca atggtggtgt
     4261 tagggatact gctagtcttg tgtggtgtcg tacaaggtgc cttggcacct gcctcacaag
     4321 ccatgattgc caattatttt ccaaataaaa cacgcggcgg agccatcgct ggatggaaca
     4381 tttctcaaaa catgggatca gccctcttgc cattaaccat cgccttgctc accagtatgg
     4441 gcttggtggt accagctaat ggcaatattt tacttgcctt tttaatccct ggcattttag
     4501 tgttcttgtt tgctttgtgt tgctggaagc ttggcggtga taaccctgag tctgaaggac
     4561 ttgactctct tcgaacgatg tatggtgatg cgggcgagtc agctgttgcc agtgaagaag
     4621 aaaagcataa cctgtcttat tggcaactca tctggaaata cgttttttgt aacccgtcgc
     4681 tcttacttgt tgctgctgtc aatgtagccc tttattttgt tcgttttggg attgaagact
     4741 ggatgccgat ctacttgtca caagtagcca atatgtcaga ggctcatatc cattttgcga
     4801 tttcaatatt agagtgggta gctattccag gctcgctggt atttgcgtgg ttagcggttc
     4861 gttatcctaa taaaatggcc aagattgggg ctattggact ttttgtgttg gcggctattg
     4921 tgtttgtcta tgaacgcttg actgccacag gtgctccaaa ctatttcttg ttgcttgtta
     4981 ttgcaggtat tttagggtca ttgatttatg gcccacagtt gatcgtgaat attttaacaa
     5041 tcaactttgt tcctttaaat gttgcaggaa cagcgattgg ttttgtagga gtaacagctt
     5101 atctcatcgg caatatggga gcaaactggc tgatgccgat tttggcagat ggttttggct
     5161 ggttttggtc atatattgtc gttgcagcct tatctgcttt ttcagcggtt ggttatttga
     5221 ttttagccaa acgtgaggaa gaaatcatca aagattagct aggcaaatgc ctaagaaaga
     5281 cagacttatt gctgaagtac tgtctttttt agtcatcaca agccgcaaat gacactataa
     5341 aagataagaa aatgctagga tgtttcagga aaaaaagaga aaatatgcta taatgagaag
     5401 gtatttaaat tatatagtaa ggaaaaacaa atgacaaatt atgccattat tttagcagcg
     5461 ggcaaaggga ctcgcatgac ttctgatctc ccaaaagttc ttcataaagt atctggacta
     5521 accatgcttg agcatgtttt tcgtagtgta aaagcgatta gtcctgaaaa atccgtgacg
     5581 gttattggtc acaaatcaga aatggtacgt gctgtcttag cagatcaatc agcctttgtc
     5641 catcaaacag agcagttagg aactggccat gctgtgatga tggcagaaac acaactagaa
     5701 ggactagaag ggcatacctt ggtgattgca ggagatactc ctttgatcac tggagaaagc
     5761 ctgaaaagtc tgatcgattt tcatgtgaat cataaaaatg tcgccactat tttaacagct
     5821 acggctcaag atccatttgg ttatggtcgt attgttcgca acaaagatgg cgaagtgatc
     5881 aagattgttg agcaaaaaga cgccaatgag tatgagcaac aattaaaaga aatcaataca
     5941 ggaacctatg tgtttgataa caagcgtctc tttgaagcgc tcaaatgcat cactaccaac
     6001 aatgctcaag gagagtatta cctgactgat gttgtggcta tttttagagc aaacaaagaa
     6061 aaggtaggag cctatatcct gagagacttc aatgaaagtc ttggtgttaa tgatcgtgtg
     6121 gccttagcaa cagctgaaac agtgatgcgc caacgcatca cccaaaaaca tatggtcaat
     6181 ggggtgactt tccaaaatcc tgaaaccgtt tatattgaaa gcgatgttga gattgctcca
     6241 gacgtcctta tcgaaggaaa tgtgaccttg aaagggcgta cacatatcgg ctcaggaact
     6301 gtcttgacga atggtactta tattgtggat tctgaaattg gtcaaggaag tatcatcacc
     6361 aactcaatga tcgaatcatc tgttttagct gcaggagtta cggtaggccc ttacgcccac
     6421 cttcgaccag gaacgacctt agatagggaa gtccatatcg gtaactttgt tgaggtcaag
     6481 ggttctcaca ttggtgaaaa aacaaaggct gggcatctga cttatattgg aaacgctcag
     6541 gttgggtctt cagtcaatgt tggggctgga accatcactg tcaactacga cggccaaaac
     6601 aaatatgaaa cagtcattgg agatcacgct tttattggga gcaactcgac tctcattgca
     6661 cctttggaag ttggagataa tgctttaact gcagctggct caacgatttc taagacagta
     6721 ccagctgata gcattgttat tggtcgtagc cgtcaagtca caaaagaagg ttatgccaag
     6781 cgcctagcgc atcacccaag tcgaagcaaa taattcaaga tctatttagt aaggaggcca
     6841 ggcatgaaat ttgaagaaaa aacgcttaag agacaaacag tttttgatgg tcatattttt
     6901 aaagttgttg tggatgatgt tgaacttccc aacaatctgg ggcaatccaa gcgagaatta
     6961 atttttcatc gtggtgccgt tgctgttttg gctattactc ctgaacgtaa gattgtcctt
     7021 gtcaaacaat accgtaaggc tattgaaagt gtctcatacg agatcccagc gggtaaacta
     7081 gagattggtg aggaaggctc aaagctaaaa gctgctgcac gtgagctaga agaagaaaca
     7141 gcctatacgg ggacattgac gtttttgtat gaattttata cagcgattgg tttttgcaac
     7201 gaaaaaatca cacttttctt agcgacagac ctaacacaaa ttgccaatcc caaaccacaa
     7261 gacgacgatg aagttatcga ggtcttagag ttgacatatc aagagtgcat ggacttagtc
     7321 gctcaaggta aacttgctga cgctaaaacg ttgattgctt tgcaatacta tgccttacat
     7381 tttggaggtg agcagtgatg ggaaggccat tgttgaccga tgatatcatc gaaaaagcga
     7441 gacgaatgga aacatttgag tcagatgatg ctgtagactt tgacacaaaa gtaatgacgc
     7501 taccagaaaa agatgaaaaa gcccgcattt ataaaagccg tcggattgaa aatgccaaac
     7561 gcagccaatt gcaatctaaa ttaaatgtga ttttgatagc tgttatgctg ttaattgcca
     7621 tcttggtata tgctatcttt tatctttaaa aaaacgtaag gaaatgatat gaaaatagga
     7681 attattgctg ctatggagga ggagttgtcc ctgctcttag caaatctttt ggatgctcaa
     7741 gaacatcagg tgctttcaaa aacttactat acgggtcgtt ttggcaagca tgagctcatt
     7801 ttggtacagt caggtgttgg aaaagtgatg tctgctatga cagttgcgat cttggttgag
     7861 catttcaaag cccaagctat tatcaataca ggatcagcag gggcagtcgc ttcacattta
     7921 gcgatcggag atgtggtggt tgcagatcgg ttagtgtatc atgatgtgga tgctactgca
     7981 tttggttatg cttatggtca aatggctggt caaccattat actatgattg tgatccacag
     8041 tttgtggcta tcttcaaaca agtcctaaaa cacgaaaaga caaatggcca agttggtttg
     8101 attgcaacag gagacagttt tgttgctggt caggacaaga ttgaccagat caaaacagct
     8161 tttagcgacg tgttagcagt agaaatggaa ggcgcagcta ttgctcaagc agcacatact
     8221 gcaggtaaac ctttcatcgt tgttcgtgcg atgagtgata ccgcagcgca tgatgccaat
     8281 attacttttg atcaatttat tatagaagct ggcaagcgtt cggcccagac attaatgaca
     8341 tttttagaaa acttacctgt ttaactcacc ttatcgtgag acaattagct ggataatctc
     8401 tattttagga gtctggtcac ctcttgtttt gaaacctaaa aataagaatt gcaatcactt
     8461 gaccaaacgc tataatgggt taaggaacga tagattacta aaaggagtct aaaaatgaca
     8521 ctaaaaaaac actattatct tctcagtctg ctagctcttg taacggttgg tgctgccttt
     8581 aacacaagcc agagtgtcag tgcacaagtt tatagcaatg gaggatatcg tcaaggtttg
     8641 actgatgaaa aatcaaagct gcaatatagt aaagacaatg ccaaacttca attgagaaat
     8701 atccttaacg gctaccaaag tgacctaggg aaacactact ctagctatta ttactacaac
     8761 ctaagaaccg ttatgggact atcaagtgag caagacattg aagaacacta taaaaagctt
     8821 aagaacaagc tacatgatat gtacaatcat tattaagtca aaggcttaaa aatggcgact
     8881 agcccctcaa aaaaagaggc cacactcatt taaatttaaa tgagtgtggc ctctttttga
     8941 ggagacgcaa ggggttaaag agctgtgaca tagagttgct tagcaatatt ttcagggatg
     9001 acaaggtcct tgtcgtcata acaaatcgta taggtttttg caaaggtatc aatttgagtt
     9061 aaggttagct cggtattgat attgaggtga tgtgcttcta gatactggat taaatcaaag
     9121 tggtcgtgga ttctgcttag gcgaaaccgt cccaactccg tgatggtgtt gagagttgta
     9181 gtgttcattt cgacaagagg ctgaccatag cgaggaatag tccctccgtg tgggcagaaa
     9241 tctggaaaat taaggatctt gtctaaacgg tcgataaagg tgtctgagac agtgtgctct
     9301 aagacttcag cttcttggtg cacttcttgg gtattatagc ccagctggtg aatcaaaaat
     9361 acctcaatca agcggtgttt gcggtagagg ttagcaacaa gagcatagcc tttgtctttt
     9421 agcaaatagc cttttgcttt atctttgaca atccagcctt gtgaaatcat ttttttaatc
     9481 atctctgaaa cggcaggtgc cgaaacgtgc atcttttctg caaccatttt atttgtaatc
     9541 ttgggttctt gttctccaat ttcataaata catttcaagt aatcttcttt attaggcgtc
     9601 atatcgtttg gcctttctct tatttataat gattattata tcagaatttt taggaaaaag
     9661 cttgacaaaa agcctaaaag aggatatcct tatagcataa ttaactaaac ttaattaaat
     9721 aaataatata tcctaaggag agaaatgatg ggaaagagaa tgagcctcat tttgggtgct
     9781 tttttatctg tttttctttt agtagcttgt tcgtcaactg gcactaaaac tgctaagagt
     9841 gataaattaa aagtcgtggc aaccaattca attattgccg acatgacaaa agctattgct
     9901 ggtgataaaa tcgatctgca cagcattgtg ccaatcggtc aagatcctca tgagtacgaa
     9961 ccattaccag aagatgttga aaaaacaagt aatgctgatg tgattttcta taatggtatc
    10021 aatctagaag atggtgggca agcttggttc accaaactag tgaaaaatgc tcaaaaaacg
    10081 aaaaacaaag attactttgc cgtgtctgat ggcattgatg tgatttactt ggaaggtgca
    10141 agcgaaaaag gaaaagaaga tccacatgct tggttaaatc tcgaaaacgg aatcatttat
    10201 tcaaaaaaca ttgccaaaca attgattgca aaggatccta aaaacaaaga aacttatgaa
    10261 aagaacctaa aagcttatgt ggctaaattg gaaaaactag acaaagaagc caaatcaaaa
    10321 tttgatgcta ttgcagaaaa taaaaaattg attgtgacta gtgaaggctg cttcaagtac
    10381 ttttcaaaag cttacggtgt cccatctgct tatatctggg aaattaacac cgaagaagaa
    10441 ggaacaccag atcaaatttc atcattgatt gaaaaactaa aagtcatcaa gccatctgcg
    10501 ctttttgtag agtcaagtgt cgatagacgc cctatggaaa ctgtttctaa agatagtggt
    10561 attcctattt attctgagat ctttacagat tcaattgcta aaaaaggtaa acctggcgat
    10621 agttattatg ctatgatgaa atggaacctt gacaaaattt ctgaaggtct agcaaaataa
    10681 gataacaata aagcacagcg attttagtgt tgtgctttgt tttatttatg taaaggagac
    10741 aaaatgatta caactaacaa tctttgtgtg acctacgatg gcaacagtaa tgctttagaa
    10801 gcgattaatg tgacaattga aggtccttct atcgttggta ttatcggtcc taatggagct
    10861 ggaaaatcaa cctttatgaa agctatctta aatcttattg attatcaagg tcacgtaacg
    10921 gttgatggca aagatggtcg taagcttggg catacagtag cttacgtaga acaacgaagc
    10981 atgattgact ataattttcc tattactgtt aaagaatgtg tggcgctagg gacttatagt
    11041 aaattaggcc tttttcgccg agtaggaaaa aaacaatttg agcaggttga taaggttctc
    11101 aaacaagttg gtttagaaga ctttggccat cgaccgataa aatccttatc tggtggtcag
    11161 tttcagcgga tgttggttgc gcgttgcttg attcaagaat cagactatat tttcttagat
    11221 gaaccgtttg ttggcattga ctcggtcagc gaaaaaatca ttgtagatct tttgaaagag
    11281 ctcaaaatgg ctggcaaaac gattttaatt gtacatcatg atttgagcaa agtagagcat
    11341 tattttgaca aactcatgat tttaaataaa catttggtgg cttatggcaa cgtttgtgag
    11401 gtatttactg ttgatacctt gtcaaaagct tatggcaatc atttgatttt aggaaaggag
    11461 atggtgtaaa tgtctatgaa attttttgag ggcttgatgt cataccactt tttacaaaat
    11521 gcactgataa cggctgtagt cattggtatc gtctcaggtg ctgtagggtg ttttattatc
    11581 cttaggtcaa tgtctcttat gggtgatgcc atctcacacg ctgttttacc aggggttgct
    11641 ttgtcattta ttttaggagt caattttttt attggagcga ttatttttgg gttattagct
    11701 tctgttatta ttacttatat caaggaaaac tctgttatta aaggagatac ggctattggt
    11761 atcaccttta gctctttttt ggcactggga gtcatcttga taggggtagc caatagttcg
    11821 acggacttat ttcatatttt gtttgggaat attttagctg tgcaagatag cgataagtgg
    11881 attactattg gtgtttcgat ttttgtttta ggggttatta gtctcttttt caaagaacta
    11941 ttattaacat catttgatcc tatcttagcc aaatcaatgg gtgtgaaggt caatgcgtat
    12001 cattatttgt tgatggttct attaacttta gtggctgtta cggcgatgca aagcgtgggt
    12061 actatcttga ttgttgcctt actgattacg ccagcagcga cagcttattt atatgctaat
    12121 agcttaaaag taatgttagt gatgtcatct ttactaggtg ctttagcatc ggttttaggg
    12181 ctttatttgg gctatacctt taatgttgcc gcagggtcaa gtatcgtact gacttctgcg
    12241 atgatgtttt tgatcagttt ctttgtttca cccaagcaag gctacctcaa aagatggatg
    12301 caaaaaaaag aaaaaacacc ttaaactttt cgctttaaac accttagtgg tttgcaaata
    12361 gtggcaaatg acatcaaagg gtgtttttta tatgtcatca aaagaccata aaaaacctta
    12421 tcaaaccaag gtatttagcc tattatctga taaggttttt ttaagcatga tttagttttt
    12481 aaagcgataa tctttgacaa tgtcaattga ggtaatcgta atgtcttgtt ctggtttatc
    12541 attttggttg atagaagtcg cggcaatttt atcgacaaca tccataccat caatgacttg
    12601 accaaataca gtataaccgc catctaagct tggattgccg ccatgctcat aggcagagat
    12661 gataggtttt gggtagttgg tacttgataa tcccttgctt tgatttttct tgttttggtt
    12721 gatataaaat tggctaccgt tcgtattagc gccagcattt gccatggcaa gagcaccgcg
    12781 aatatgatat aaaaatggag agatttcgtt gacaaagcca ttgccagcat cttttttagg
    12841 atccttgccc ttccaaatcg attcaccacc tgtgccatct cctttggggt cacctgattg
    12901 aatcataaag tcgttgatca cacgatggaa ggtaaggtta tcatagtagc cttttttagc
    12961 atgggtgagg aagttctcaa cagctaaggg agcatatttt gggaataact taagggtgat
    13021 gtccccttga cttgtcctca ttacgacctt agcttcttct ttgccgactt ctttggttag
    13081 ttgtggaaat tggctagcat cagctttgag agctttttga atgctttctt cataggcttt
    13141 agaagctgct tcgctttctt ctttggcagt tttttcatca atgtatttat cgccttttat
    13201 agcgcggtca acagattcac aagcactcaa actaagtaaa ctaatggcta ctaaactgag
    13261 agataataat tttttcatgt ctactcctaa gaatgatttt ctttatttta tcataaaatg
    13321 caacagaaaa tagtagtaaa gagttaaaaa cgcctatttt tgtgttttat gatctaacct
    13381 tggttaaaag gctgctacga ctgttattat agcacaagat gctatagaaa aacttaaaac
    13441 accttggttt tttagagcta tttcctaata gttaaaaaat ggtataatag tgttatggtt
    13501 aaaagaaatc aaagaaaaaa aagtgcgcct aagaagcgtt taacaaaagc agaagtggaa
    13561 aagcaacgtg ccattaagag gatgatttta tctgttttaa tggctttgtt gcttattttt
    13621 gcgatgcttc gcttgggcgt ctttggcgtg acaacttata atatgattcg ttttttggtg
    13681 ggtagtttag cctatccgtt tatgtttgcc tggttaattt acttattttg ttttaaatgg
    13741 ttacggcaaa aagatggaat gattgcggga gttgttattg cctttttggg attgctagtt
    13801 gagtggcacg ccttcttatt tgcgatgcct aggatgcttg atcaagatat ttttctaggc
    13861 actgctcggc taattacaag agatttacta gccctgcgcg tgactgaatt tgttggtgga
    13921 ggaatgcttg gtgcattact ttataaaccc atagcctttt tattttcgaa tatcggctct
    13981 tactttattg gttttttgtt tatcttatta ggattatttc taatgacgcc ttgggatatc
    14041 tatgatgtga gtcactttgt gaaagaggct gttgataaac tagcagtggc ttatcaggaa
    14101 aataaagaaa agcggttcat caaacgagaa gagcaccgct tacaagctga aaaagaggct
    14161 ttagagaaac aagcacaaga ggaagaaaaa cgtctagcag aattgacggt tgatcctgag
    14221 acgggagaga ttgttgagga tagtcagtct caagtcagct atgatttggc tgaggatatg
    14281 ccaaaagagc cagagattct tgcttatgat agtcacctca aagacgatga ggcaagctta
    14341 tttgaccaag aagacttggc ttacgctcat gaggaaattg gggcttatga ctctttatct
    14401 gctttagcat cttctgaaga tgagatggat atggatgaac ctgttgaagt tgatttcaca
    14461 ccaaaaaccc atcttcttta taaacttcca acgatagatt tatttgcacc agataggccc
    14521 aaaaaccagt ccaaagagaa aaacttagtc cgcaaaaata tcaaggtgct tgaagatacc
    14581 ttccaaagtt ttgggattga tgtcaaggta gaacgtgctg aaattggacc atcagttact
    14641 aaatatgaaa tcaagccagc tgtcggggtc cgagttaatc gtatttcaaa tcttgctgat
    14701 gatttggcac ttgctctggc tgctaaagat gttcgtattg aagcgccaat tcctggaaaa
    14761 tccttaatag gtattgaagt tcctaactca gaaattgcaa cggtttcttt ccgcgaactt
    14821 tgggaacaat ctgatgccaa tcctgaaaac cttttagaag taccactagg taaagctgtt
    14881 aatggcaacg ctcgcagttt taacttagct agaatgccgc atcttttggt agctggttca
    14941 actggttcag gtaaatctgt ggcagttaat ggcattattt caagtatttt gatgaaggca
    15001 cgtccagatc aagttaagtt tatgatgatt gaccccaaaa tggttgaatt atctgtttat
    15061 aatgatattc cacatctatt aatcccagtt gtaaccaacc cgcgtaaagc cagtaaggca
    15121 ctccaaaaag tggttgatga gatggaaaac cgctacgagt tatttagcaa aattggtgtg
    15181 cgtaatatag caggttataa tacaaaggtt gaagagttta atgcttcttc tgagcaaaaa
    15241 caaataccgt tgcctttaat cgttgtcatt gtagatgaat tggctgactt gatgatggtg
    15301 gctagtaaag aagttgaaga tgctattatt cgtttgggac aaaaagcacg tgctgcaggt
    15361 atccatatga ttcttgcaac tcaacgtccc tctgtagatg ttatttctgg tttgattaaa
    15421 gcaaatgttc cgtcgcgtat ggcatttgct gtttcaagtg gtactgatag ccgtacaatc
    15481 cttgatgaaa atggtgctga aaagctcttg ggacggggtg atatgctctt taagccgatt
    15541 gatgaaaatc accctgttcg cttgcaaggg tctttcatct cagatgatga tgtggagcgt
    15601 attgtcaact ttatcaaaga ccaagctgaa gcggattatg atgacgcctt tgatcctggt
    15661 gaagttagcg acaatgatcc tggtttttct ggaaatggcg gatctgctga aggtgatcct
    15721 ctttttgaag aagcaaaagc cttggtctta gaaacacaaa aagctagtgc ctcaatgatt
    15781 caaaggcgtt tgtcggttgg atttaatcgt gccacgcgct tgatggatga attggaagaa
    15841 gcaggtgtta tcggtccagc agaaggaacc aaacctcgta aggtattgca aacaaactaa
    15901 aagaacctga cgtctcatga tgagatgtca ggttcttttt ttgtgatgtc aggccagttg
    15961 atgagatgcc tatgctaatg taaaaataaa gataactaga ataaggtaga aaataacggt
    16021 gactaaaaag ccaactcgcc aaaatagctt cataaagcga tggtaagaaa acgactgagt
    16081 tcggatgact agcagtagtg aaatgacgat ggctagcagc gacatgataa taaggtaata
    16141 aggcagtaaa ttatgggtga aaaattttcc tgagacaagc acaatttcta ccgcaaaaag
    16201 taaaagtgcg atatctggaa atttaaatcc ttttttatta agtttaaata aagaggttac
    16261 aatcatggca aaaagaggcg ttactatcaa gaaagctaag gcagttaatt tatagatcat
    16321 cataggtcta ttttatactc aaatccctta cttgtaaact gatacttacc tgttgtgata
    16381 gaaaatgatc tttgagaggg aaagcgccta cttcaattta aacattgcaa aaggcatcag
    16441 ctatttt
//
LOCUS       DAUGFP010000034        16063 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.034, whole genome shotgun sequence.
ACCESSION   DAUGFP010000034 DAUGFP010000000
VERSION     DAUGFP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16063
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.034"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..639
                     /locus_tag="VH663_001184"
     CDS             <1..639
                     /locus_tag="VH663_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ8174387.1"
                     /translation="RGETGDKGEQGPAGKDGDRGPVGPAGKDGQDGKDGLPGKDGKDG
                     QDGKDGLPGKDGKDGKDGQDGKDGLPGKDGKDGQNGKDGLPGKDGKDGQNGKDGLPGK
                     DGKDGQPGKPAPKTPEVPQKPDTAPHTPKTPQIPGQSKDVTPAPQNPSNRGLNKPQTQ
                     GGNQLAKTPAAHDTHRQLPATGETTNPFFTAAAVAIMTTAGVVAVAKRQENN"
     gene            839..1276
                     /gene="msrB"
                     /locus_tag="VH663_001185"
     CDS             839..1276
                     /gene="msrB"
                     /locus_tag="VH663_001185"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ8174388.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGEYDNFFEKGIYVDI
                     VSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYDQMEKEGYAQWLTLFDET"
     gene            1406..2425
                     /locus_tag="VH663_001186"
     CDS             1406..2425
                     /locus_tag="VH663_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEQ8174389.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWCLGRLFPIIGAPVFAILLGMLL
                     ALFYEHRDKTKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGSQKWLRLDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNEGFAIFAGTAVNDTSSVTATATAWDALHHSNTLNGATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEENFSLRKSFPRFILFFLLASLITTLMTSLGVS
                     ADSFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKMGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            2632..3057
                     /locus_tag="VH663_001187"
     CDS             2632..3057
                     /locus_tag="VH663_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ8174390.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDDFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDEIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            3076..3567
                     /locus_tag="VH663_001188"
     CDS             3076..3567
                     /locus_tag="VH663_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ8174391.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRATDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            3584..4393
                     /locus_tag="VH663_001189"
     CDS             3584..4393
                     /locus_tag="VH663_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ8174392.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            4390..5217
                     /locus_tag="VH663_001190"
     CDS             4390..5217
                     /locus_tag="VH663_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ8174393.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            5353..7002
                     /locus_tag="VH663_001191"
     CDS             5353..7002
                     /locus_tag="VH663_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8174394.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDDLGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPSQDLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            7006..7794
                     /locus_tag="VH663_001192"
     CDS             7006..7794
                     /locus_tag="VH663_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8174395.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            7788..8834
                     /locus_tag="VH663_001193"
     CDS             7788..8834
                     /locus_tag="VH663_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ8174396.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            8949..10346
                     /locus_tag="VH663_001194"
     CDS             8949..10346
                     /locus_tag="VH663_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEQ8174397.1"
                     /translation="MAYQTIYPYTNEVLHTFDNMTDQGLADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVNLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGIKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            10448..12244
                     /gene="uvrC"
                     /locus_tag="VH663_001195"
     CDS             10448..12244
                     /gene="uvrC"
                     /locus_tag="VH663_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEQ8174398.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLRIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            12429..13031
                     /locus_tag="VH663_001196"
     CDS             12429..13031
                     /locus_tag="VH663_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEQ8174399.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            13156..14565
                     /gene="pepV"
                     /locus_tag="VH663_001197"
     CDS             13156..14565
                     /gene="pepV"
                     /locus_tag="VH663_001197"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEQ8174400.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDQLADDKHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFAHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNKGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLNVLEAALEQFLSEHGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGVNGATLLAKFLNQFTFEGAAKDYLHVAGEVLHEDFAAEKLGLAYTDDRMGTLSMNA
                     GVFTFDNQSADNTIALNFRYPKGTDAATLKAGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(14633..16009)
                     /gene="mnmE"
                     /locus_tag="VH663_001198"
     CDS             complement(14633..16009)
                     /gene="mnmE"
                     /locus_tag="VH663_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEQ8174401.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQDSNRI
                     ILLNKTDLEQKIELEQLPADLIPISVLTNHNIDLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
BASE COUNT         4932 a         2951 c         3358 g         4822 t
ORIGIN      
        1 cgcggcgaaa ctggtgacaa aggagaacaa ggtcccgctg gaaaagatgg tgaccgtggt
       61 ccagtaggtc cagctggtaa ggacggccaa gacggtaaag atggtctccc aggtaaagac
      121 ggtaaggacg gccaagatgg caaagatggt ctcccaggta aagacggtaa agacggtaag
      181 gacggccaag acggtaaaga tggtctccca ggtaaagacg gcaaggacgg ccaaaatggt
      241 aaagatggtc ttccaggtaa agacggcaag gacggccaaa atggtaaaga tggtcttcca
      301 ggtaaagacg gtaaggacgg tcaaccaggt aaaccggctc ctaaaacacc agaggtccct
      361 caaaaaccag ataccgcacc acatactcca aaaacccctc agatccctgg ccaatcaaaa
      421 gacgtgacac ctgctcctca aaacccttct aatagaggtc taaacaaacc acaaacacaa
      481 ggtggtaatc agctcgcaaa aacaccagca gctcacgaca cacacagaca attgccagca
      541 acaggcgaaa caaccaatcc attctttaca gcagctgctg tagctatcat gacaacggct
      601 ggagttgtag ctgttgcaaa acgtcaagaa aacaactaat cctctttttg gctacctaac
      661 tgatggaaag taaaactcag agagaacctc aaggttctct cttttttgaa tcaagttttt
      721 gaatcaagat agtagctcta ggtataacgt cttgtctgag gtgctgtgaa caaatcagtt
      781 accactcacc tctttttttg gtaaaatgta agcactaaaa catcaaaagg agttttccat
      841 ggaaacatca gatgagttaa aacaacgcat tggtgatctt tcttacgagg tcacccagca
      901 tgcagcaaca gaatctccct ttacaggaga atatgataat ttttttgaga agggtattta
      961 tgtcgatatc gtcagtggtg aggtcttgtt ttcatcactg gataaattta attctggctg
     1021 tggttggcct gccttttcaa aacccattga aaatcgtatg gtcacaaacc acgatgactc
     1081 ttcttatggg atgagacgag ttgaggttaa aagccgtgaa gcaggatcgc atttggggca
     1141 tgtctttagt gacggcccca aagaagctgg aggcttgcgt tactgtatca attcagcagc
     1201 tcttaaattc attccttatg accagatgga aaaagaaggc tatgcccaat ggctcacatt
     1261 gtttgatgag acataacagg gtttcaaaat gactaaaaaa gaagcgtcag tgattcccac
     1321 aagcactgga ttggtgaggc gtacgggtca attttacaag cctttgtaaa aggtctataa
     1381 tagggaatga aaaaaggagg ttagtatgtc aactcactta agaaaacttc cagggctgtt
     1441 actttgctta ttgttagctc ttccagcctg gtgtttaggg cgcttgtttc ctattattgg
     1501 agcacctgtt tttgctattc ttttaggaat gttgttagcc ttgttttatg aacatcgtga
     1561 caagactaaa gagggaatta gttttacatc caagtatatt ttacaaacag cagtggtttt
     1621 gcttggtttt ggattaaacc taacccaagt tatggcagta ggcatgcagt ctttaccgat
     1681 tatcatttca actattgcga cagctctttt ggtagcttat ggctcacaga aatggctgcg
     1741 cttagatgtc aatacagcca ccttggtggg tgtagggtct tccatttgtg gggggtctgc
     1801 tattgcagcg acagctcctg tcattaaggc aaaggatgac gaggttgcta aggcaatttc
     1861 agtcattttt ctctttaata tgttagcagc tttgctattt ccaagtttag gacaattact
     1921 aggcttatct aatgaaggtt ttgctatttt tgctgggaca gctgttaacg acacctcttc
     1981 cgtgactgca acggcgacgg cctgggatgc ccttcaccat tccaatacac taaatggcgc
     2041 aaccattgtc aaattgactc gcaccttggc tattcttcca attactttag gtttatccct
     2101 ttaccgagcg aaaaaagagc acgacatcgt tacagaagaa aactttagcc ttaggaagtc
     2161 attccctcgt ttcatcctct tctttttatt agcttccctc atcacgacat tgatgaccag
     2221 tttgggagtt tctgccgata gtttccattc cctaaaaacc ttatcaaaat tctttatcgt
     2281 gatggctatg gcagctatcg gtttaaatac aaacctggtt aaactgatta agatgggcgg
     2341 tcaggctatc cttttaggag ctatttgctg ggtagctatc acccttgtca gtttagccat
     2401 gcaattaagt ttgggcattt ggtaatcaga cacgagaaat cagtttgact gatttctttt
     2461 tttgtatctt atttgtgtag aattgtctac attttaaaga gaataatgca ttttaaaaac
     2521 gttttcattt gataaaataa acctatcgtt aaaccagttc agtaaggaca actggttgag
     2581 ataagcaatt accgtttact cagcggtttt tgttaataga ggaggatagg aatgaaacga
     2641 aaatttctca ttgggagtca cggcagacta gccagtggtt tgcaaagctc tattgacatt
     2701 ttagcaggta tgggacaggc acttgaaacc attgatgctt atgttgatga tagtgattat
     2761 actagccaga ttgatgactt tatcgctggg gtagcagcag atgagcaggg ccttattttt
     2821 actgatttgt taggcggcag tgtgaatcag aaaatggtaa ccgctgtaat gaacagtggc
     2881 aaagacaata tctttttaat cacaaacagt aacttagcca ctttattgtc gttggtattt
     2941 ttaaaaccag gcgaagcgct tactaaagat gaaattgtaa ccgttatcaa tgaatcgcaa
     3001 gttcagttag tggatttggt ccctgagaca aattcagaag atgacttttt tgattaatat
     3061 ctaggaggaa gacaaatgat tactcaaatt cgtgttgacg accgtttgat tcacgggcaa
     3121 gtggcagttg tatggaccaa agagttaaat gcgccacttt tagtagtggc taatgatgaa
     3181 gctgcaaaaa atgaaatcac acaaatgacg cttaaaatgg ctgtgccaaa cggcatgaaa
     3241 ctgcttattc gttcagttga agactcgatt aagctcttta atgatccacg cgcaactgac
     3301 aagcgtattt ttgtgattgt caattctgtt aaagatgctt gtgccattgc taaagaagtt
     3361 ccagacttag aagctgttaa tgtggctaat gtggggcgct ttgataagtc agatccagca
     3421 agcaaggtta aggtgacgcc aagtctttta ttaaacccag aagaaatggc agccgcaaag
     3481 gaattagtca gtttaccaga acttgatgtc tttaaccaag tcttaccatc aaataccaag
     3541 gtccatttga gccaattagt caactaaaga gaggtaaaaa agtatgttag taccagcaac
     3601 aatggcagct ttagctgtat tgatttgttt tgggggaaat tatttaaccg gtcaaagcat
     3661 gatggaaaga cccctagtag ttggactggt cacaggtctt cttttaggag atatgaaggt
     3721 cggtatctta atgggggcct ctctagaagc tcttttccta ggaaatgtga atattggggg
     3781 tgttattgca gctgagcctg taacagcgac tgccatggca acaaccttta ccattatttc
     3841 acatattgac caaaaagcag ccatgaccct agctgttcca attggaatgt tagcagcctt
     3901 tgtggttatg tttttgaaaa acgtcttcat gaatatcttt gctccaatgg ttgataaagc
     3961 tgcagcagct aaccatcaag gtaaactagt gatgctccac tatggcactt ggattatcta
     4021 ttacttaatc attgcctcta tttctttcat tggtatttta gtaggtagtg gacctgttaa
     4081 tgcctttgtg gaacacattc ctcaaaatct catgaatggc ttgagtgctg cgggaggcct
     4141 tcttccagct gtcggttttg ccatgctgat gaaattattg tggaccaaca aattagctgt
     4201 tttttaccta ttgggctttg tcttaacagc ttatttgaaa ttaccagcgg ttgcagttgc
     4261 tgccctagga gcagttattt gcgtcattag ttcccaacgt gatctagaat tagacgcaat
     4321 tactagaggt gcaattagta aacaaacaac ctttgactct aaagaatcag aagaggagga
     4381 cttcttcgca tgacatcaca agataatttg actaaagaag accgtaaaat gcttcgctca
     4441 gtcttttggc gctcatggac gatgaatgct agtcgtactg gggcaacaca ataccacgca
     4501 gtaggggtta tttacacttt actaccagtc atcaatcgtt tttataagac tgataaggat
     4561 aaagcagagg cactggtccg tcatacaaca tggttcaatg ccaccatgca tatcaataac
     4621 tttatcatgg gtcttgtggc atcgatggaa aagaaaaaca gtgaagatcc tgattttgat
     4681 gctagtgcta ttacggcggt aaaagcatct ctaatgggac ctatttccgg cgttggagat
     4741 tccttcttct ggggaatttt aagggttatt gctgcaggaa ttgggatttc cttggcaagt
     4801 gcaggctcag ccatgggagc tgttgtcttc ttactcttgt acaatattcc tgcttttatt
     4861 attcactact atagccttta tggtggttat tctgtaggtg caggatttat caaaaaactt
     4921 tacgaatctg gtggtataaa aatcgtcact aaaacctcaa gcatgcttgg gttaatgatg
     4981 gtaggctcaa tgactgcttc aaatgtaaaa tttaaaacga ttcttacagt tgctgcgaaa
     5041 ggtgccaaag aagcagcttc tattcaagat taccttgacc aattgtttat tggtattgta
     5101 ccattaatgg taacactagc agctttttgg cttctacgaa agaaagtgaa tatcaactgg
     5161 attatgtttg gtatcatgtt ccttggcatt atccttggac ttttaggtat ttgctaataa
     5221 tctcctagca ataaaggtaa gtaagacaag ttttcttgtt ttgcttacct ttttcctttt
     5281 aagatgatga gacttgtttt gatgaaaaac gtagtagcat taagaaattt gttataatga
     5341 aaggagctta agatgcgagg tgaacaggtg gaagaacatt ttaaaaaaca attacaagat
     5401 gatatttcta gacatttttc gtaccaatcg ttaatgttat cattgttgct aattggtctt
     5461 tttattattt tttcattagc gccacaacaa ttaggcctct atcgtgatat caatgccact
     5521 gcaacccgtt atcaccgttt gattagcaaa caagaagcct tgcttgatga cttagggaaa
     5581 aatagcttac tacctttttt aaataaaaac ctcagcactg ctgatttaag caagcactat
     5641 tttcatttgc gccatagcag ccaaacgtca ccagaacttt tactattttc acctagtcaa
     5701 gaccttttat ttgctagtaa cccacattta ggaaatgttt ttagtaaatc tgtttatatt
     5761 caagaagtct tgagagcagc tcatcctcca aaaaccttgt ttaaagttgc tatggatagt
     5821 gaagatggtc actacttgat gatcattaag ccaatgattg atcaaaacca actaaaggga
     5881 tatgcttttt tagtgatgag tggcaaagat tttcttcatc ctacaaaaac attgacgtca
     5941 gagttggtca ttgctgataa gctggacaat acgtttacgt tttccaatcg tgagtttata
     6001 gcatctagtc tagataagat caatagccag tatttacacc attattttgt ttttcaagat
     6061 aatcgagcct ttatcacaag aaaggttgcc ttacaaggag gtctttggct ttacatgtat
     6121 aggcccttga ttccaatggt atcggtgatg ttattttcac ttatttcatc agctgttatc
     6181 tttgtgatct tgcaacgtaa atcaagtggt ttagctaacc gaattgcagc taaaaattca
     6241 agagcaatca atcaaatggt tagagatatg agtgcgatct ctcggcaaga aaaaagacgt
     6301 attgaccttg agagtcaaga tgaatttcaa tatttatctg atcaaatcaa tcaaatggta
     6361 gagcgattgc aacagttaca tgataaaacg ttagatttgg aaactcaaaa attattattt
     6421 gaaaaacgga tgttagaagc tcaattcaat ccgcatttcc tctacaatac gctagaaacc
     6481 attttgatta cgagccatta tgattctgcc ctaacagaaa aaattgttat ccaattgaca
     6541 aaattgttgc ggtatagtct cacggattct agcaaacctg tcttgctcaa agatgattta
     6601 agtgtcatcg agtcttattt agtgattaat caggtgcggt ttgaagaatt gcaatatagc
     6661 attaacctat ccccagacct tgatagtctt gcagtgccta aattgttctt gttacctttg
     6721 atagaaaatg ccatcaaata tggcttgaaa gaacgacacg atgtcaagat taacattgct
     6781 tgttattatc aagatgacca cattattttt tctgtgcgag ataatggttc gggaattgat
     6841 gctcatcacc aaaaagtgat tcgagagcaa ttggaagctg gagagtcaca ccatgggtta
     6901 attaactctt atcgtcggct taagtatcat ttttcagaag tatccttggt ttttgatcaa
     6961 ggtgataaac agtttaatgt tagttaccat gttaaggagt agtctatgta caaattagtc
     7021 attattgaag atgaacatat tattaggaaa tggttgcgat acgctattga ttataaagct
     7081 ttagatattt tggtcattgg cgaggctaaa gatggcaaag aaggagctgt tttaatcaaa
     7141 gagagtcaac cagatatcgt tttaaccgat attaacatgc ccatcatgac agcttttgac
     7201 atgtttgaag caacaaaaga tcaaacttac gccaaaatta ttttatctgg ctatgccgat
     7261 tttccaaatg caaggtctgc catacactac ggtgtacttg aatttttaac aaaaccgatt
     7321 gaaaaagcag ctctttggga atgtcttcaa accattattg ctaaaataga aaagcaaaag
     7381 gggtctaatc aaaaaacaga tgcttgcgtc tatattccct taccccagat gactgatcag
     7441 attcctgaag tagtcaaaga tattttggag tgggtacatg ctcattttca agacaagatc
     7501 agcacgagcc gcctagctca tgacttaggg tatagtgaga gttatatcta tcaaaatatc
     7561 aaaaagcatc ttcagatgcc tttaagtgat tacattaatc aatatcggat taatcaggca
     7621 attcaattga tgcaacaaga accagattta atggtatatg aaatagcaca agctgtaggt
     7681 atttatgact atcgctattt tgatagggtt tttaaaaagt atctaggaca aactgtaaag
     7741 gcttttaaag aagagcattt tatgaaagat accgatagga gtaagccatg ttaaaactca
     7801 aacgcaaatg gctattaagc tttttgttag taataattat tctagcaatc gttaacattg
     7861 caatgtatat attctcatcg tcaaaaaaag atagtgctaa agaattagtt atcttgactc
     7921 ctaatagcca aactatttta acagggacta ttccagcctt tgaggaaaag tatggggtta
     7981 aagtaagatt aatccaaggt gggacgggcc aacttattga tcaattaggt cgaaaagata
     8041 agccattaaa cgctgatatt ttctttggtg gcaattacac tcaatttgaa agccataaag
     8101 atttatttga atcctatgtt tctccgcagg tttctactgt catttcagat taccaattgc
     8161 ctagtcatcg tgcaacccca tatacgatca atggcagtgt actgattgtt aataacgaat
     8221 tagcaagagg acttcatatt accagttatg aggatttgct acaaccagct ttaaaaggca
     8281 aaattgcttt tgctgaccca aatagctcat ctagcgcttt ttcacaatta acaaacatct
     8341 tactggctaa aggaggctat acgagctcag aagcttggga gtatgtgaaa cgtttattac
     8401 tgtctatgaa ttctatcaga gcaacaagtt cttcagatgt ttacgaatct gttgcagaag
     8461 gtaaaatgat tgttggtttg acctatgaag acccttgcat caatctgcaa aaaagcggtg
     8521 caaacgtttc gattgtttat cctgatgagg ggacagtatt tgttccgtca tcagtcgcta
     8581 ttatcaaaaa agcaaaggca atgaaagaag ctaagcaatt tattaatttt attttatcat
     8641 cagatgtaca aaatgccttt gggcagtcaa ctagtaatag acctatccga cgagatgctc
     8701 aaacgagtca tgaaatgaaa ccattagatg aaattattac tttaaaagaa gattacggat
     8761 atgttactag acataagaaa aaaatattga atacctataa tcgactacat gaagcattag
     8821 aaagaggaaa gtagactcta ggcttgttgt tgctatacta gtagtgttac aaaatagcgt
     8881 agtcattttt tgaaatcgat ttcataatct ggtagactaa ggttatcacg aaaaggaagc
     8941 tatatgtgat ggcttatcaa actatttacc cttatacaaa tgaagtcctt catacatttg
     9001 ataatatgac cgatcaaggt ttggcagatg tccttgaaag agctcacctt ctctataaaa
     9061 aatggcgcaa ggaagatcat ttagaagaac gtaaagccca gcttcatcaa gtggctaata
     9121 ttcttcgtcg tgaccgcgat aaatacgctg aaatcatgac caaagacatg ggaaaactgt
     9181 tcacagaagc ccaaggtgaa gtaaatcttt gtgccgatat tgctgattac tacgctgata
     9241 aggcagatga atttttgatg tctacgccac tagaaacgga ttcaggtcaa gcttactatt
     9301 tgaaacaatc aactggtgtt atcctagctg ttgagccttg gaattttcct tactatcaaa
     9361 tcatgcgggt ctttgcgcca aactttattg ttggaaaccc aatggtttta aaacacgcgt
     9421 ccatttgccc acgctctgcc caatctttcg aagaattagt tcttgaagct ggtgctgaag
     9481 caggtagtat taccaatttg tttatttctt atgaccaagt atcacaagtg attgctgata
     9541 aacgtgtggt tggtgtctgc ttgacaggtt ccgaacgtgg aggagctagc attgcagaag
     9601 aagctggtaa aaacttgaaa aaaacaaccc ttgaattggg tggcgatgat gcctttatta
     9661 tccttgatga cgcggattgg gatcaattag agaaggtgct ttacttctct cgtctctaca
     9721 atgcaggaca agtatgtacc tcatcgaaac gttttatcgt gcttgacaaa gactatgacc
     9781 gtttcaaaga gttattaaca aaagtcttca aaacagctaa atggggtgac ccaatggatc
     9841 ccgaaacgac gttggcgcca ttatcatcag ctcaagctaa agcagatgtt cttgaccaaa
     9901 ttaagttggc cttggatcat ggtgcggagt tggtttatgg tggtgaagcg attgatcatc
     9961 caggtcattt tgtcatgcca accattattg ctggcctcac aaaagacaac cctatttact
    10021 atcaagaaat ctttggccca gtcggtgaaa tctacaaagt gtcttctgaa gaagaagcga
    10081 ttgaagtggc gaatgattct aactatggcc ttggaggtac tatctttagt agcaatcaag
    10141 aacatgcgaa agccgtagct gctaaaattg aaactggtat gtcttttatc aactcaggct
    10201 ggacaagtct tccggaactc ccatttggtg ggattaaaca ttcaggctat ggccgtgaat
    10261 tgagtgagct tggctttaca tcttttgtga atgaacacct tatctatatt ccgaataaaa
    10321 cgaataatag caatacgaaa gtttgatcta gattgttaaa ggagatctgc tcgccttaat
    10381 aaacaatcaa ggcgttacac tcttcttttt taaagtcaaa attttaaaaa tttgataaac
    10441 tagtaacatg aacgaactga ttaaacataa actagaactc ttaccagaca gccctggctg
    10501 ttacttacat aaagataaag aaggaactat tatctatgtc ggtaaggcca agaatttgaa
    10561 aaaacgcgtc cgatcctatt ttcgtgggag ccatgatacc aaaacagagt tattagtatc
    10621 tgagattgta gatttcgaat atattgtaac agagtcagat accgaagcgc ttttgttgga
    10681 aattaacttg attcaaaaaa atatgcctaa gtacaacatc aagttaaaag atgataaatc
    10741 ttatcccttt ctcaaaatca caaatgagtc ttttcctcgg ttagtgatta caagatatat
    10801 taagaaaaat gatggattgt actttgggcc ctacccagat tcttacactg ctaatgaagt
    10861 taaaaagtta ttagatagga tttttccatt taaaaagtgt aaaaatccta tcaataaggt
    10921 ctgtttttat taccatttag gccagtgctg tgctcatacc atttgtcata cagacaaggc
    10981 ctattgggat cgtttaattg atgatgttaa gcacttttta aatggtaaag atgacaaaat
    11041 tattgaagat cttcgttcta agatgttagc agcgtctgaa gaaatggcat ttgaacgtgc
    11101 cgctgaatat cgagatttga tttcaggtat tgccaccatg cgcaccaagc agcgggtgat
    11161 gagcaaggat ttgcaagata gagatatctt tggctattat gttgataagg gatggatgtg
    11221 tgtccaagtt ttctttgtgc gtcaagggaa attaattcag cgtgatgtta acctttttcc
    11281 ctattataac gatgcagaag aagatttctt aacttatatg gggcaatttt accaagataa
    11341 acagcatttc atccctaaag aagtctttat tccagaagct attgatgaag aactggtagc
    11401 cgctattgtg ccaaccaaaa tcatcaaacc caaacgcggt gaaaaaaaac aactggtagc
    11461 tttggctact aagaatgctc gtgttagtct gcaacaaaaa tttgatttat tagaaaaaga
    11521 tatcaaaaaa acgagtggtg ctatcgaaaa tttaggtcag ttacttagga ttgacaagcc
    11581 tgtgcgcatt gaagcctttg ataattctaa cattcaaggg acaagtccag ttgcggctat
    11641 ggttgttttt gttgatggca aacctagtaa gaaagattat cgtaaattta agattaaaac
    11701 agtggtggga ccagatgatt atgccagtat gagagaagtg ctttttcgaa ggtacagtcg
    11761 ggtgaaaaag gagggactgc aagctcctaa cttgattatt gttgatggag gcgttggaca
    11821 agtcaatgtg gccaaagatg tgatagaaaa acaacttgga ctcactattc ctgttgcggg
    11881 acttcaaaaa aacgataagc accagaccca tgatttgctt tttggaaacc cacttgaagt
    11941 tgtaccgttg ccgcgccgtt ctgaagagtt tttcttactg catcggattc aagacgaagt
    12001 gcatcgcttt gcagttacat ttcatagaca agtgcgacgt aaaaattctt tttcatcaac
    12061 attagatcat atttcagggc ttggcccaaa gcgaaaacag ttattactaa gacactttaa
    12121 aaccataaca gcgattgcat cagctacctc tgaggagatt caagctttag gtatacctaa
    12181 aacagtagtt gaagcaatac agcaacagat aacagacaat aaaaatgata gatcgtctcc
    12241 ttaaataagt gttatgtttt cagtactgat gaggtaaaaa ggcgctactt gttacgcacg
    12301 acatttcagt tcgttatttt gaaactttca gaaagttgcg aagtgtagtg agtttatgat
    12361 tttaaaaact attttatggt aaaataaaat ggtattaaaa aaaacagatt tagataggaa
    12421 gttttgaaat gaaatttcta gagttaaaca aaaaacgcca tgccattaag acctttaatg
    12481 accagccagt tgactacgag gatttgcgaa cagccattga gattgcaacc ttagcaccta
    12541 gtgccaataa cattcaacct tggaaatttg tagtcgttca agagaaaaaa gctgagttag
    12601 caaaagggtt acctctagca aataaagtgc aggttgaaca ggctcaatat gtagtggcgc
    12661 tattttcaga cactgattta gctttacgtt ctcgtaaaat tgcccgtatc ggtgtcaaat
    12721 cattaccaga cgacttaatt ggttattata tggagacttt gccaccacgt tttgctgctt
    12781 ttaatgaggt tcaaaccggt gagtacttag ctattaatgc aggcattgtg gctatgaact
    12841 tggtgttatc cttgacggat caaaagattg cttccaatat tatccttggt tttgataagt
    12901 caacgaccaa tgagatttta gacatcgatc cgcgttttcg tccagaatta ttgattactg
    12961 ttggctatag tgacgagaaa ccagaaccaa gttatcgttt gcctgttgat gaagtcatcg
    13021 aaagaagata gagtcaggac aattagttaa atgtaatgtc cagatgcgct actgagcgac
    13081 cactattgtt agactgatac tattttggct tattttaagt taaacattat ccctaaaacg
    13141 aataaggaga gtataatgac aaccattgat tttaaagcag aagttgacaa acgtaaagaa
    13201 gctatgctag cagatttagt tgacctatta cgcattaatt cagaacgcga tgaccaatta
    13261 gctgatgata aacacccatt tggcccaggt ccagttaagg ctttggagca tttcctcgca
    13321 atggctgagc gtgatggcta caaaacgcgt aatattgata actatgcagg tgactttgaa
    13381 tttggtcaag gtgatgaagt attaggaatc tttgctcacc ttgatgttgt cccagctggt
    13441 agcggttggg atacagaccc ttatgagcca gtgattaaag acgatcgcat ctatgcgcgt
    13501 gggtcttcgg atgataaggg gccaaccatg gcgtgctact acgctctgaa aatcattaaa
    13561 gaattgggtc ttcctgtgtc taaaaaagtt cgtttcattg ttggtaccga tgaagaatct
    13621 ggctgggggg atatggatta ttactttgcc cataatggct tgaaaaatcc agattttggt
    13681 ttctcaccag atgctgaatt tccaattatc aatggtgaga aaggaaatat cactgaatac
    13741 ctccatttcg ctggtgacaa caagggcgct tttgtgcttc atcgcttcca aggtgggttg
    13801 cgtgaaaaca tggtaccaga atctgccaca gctgtcatta cggcaccgca tgaccttaat
    13861 gtgctagaag cagccttgga gcaattttta tcagaacatg gtgttaaagg ttctatgaag
    13921 gctactgatg gtaaaattga agtaactatt attggtaagt ctgctcacgg gtcgactcca
    13981 gaagcgggtg ttaatggtgc caccttgctg gctaaattcc tgaatcaatt tacctttgaa
    14041 ggggctgcta aggattattt acatgtggca ggtgaggtgc ttcatgaaga ctttgcggca
    14101 gaaaaactag gccttgctta taccgatgac cgtatgggaa ccttgagcat gaatgcgggt
    14161 gtcttcacct ttgataatca atcagctgac aataccatcg ccttgaattt ccgttatcca
    14221 aaagggactg atgcggcaac cttaaaagca ggattggaaa aattaccagg attaactaag
    14281 gtaagccttt ctgagcatga acatacacct cattatgtgc caatggatga tgaattagta
    14341 gctaccttac ttgctgttta cgaaaaacaa acaggtctta aaggctacga acaagttatc
    14401 ggcggaggaa cctttggtcg tttgctagaa cgtggtgtgg cctttggagc gatgttccca
    14461 ggtgatgaaa acactatgca ccaagctaat gaatatatgc ctttagaaaa tatttatcgc
    14521 tcagcagcta tttatgctga agcaatctac gaattaatta aataagatgc ttatgacagc
    14581 aaaaagagct ggaacactgt tccagctctt ttgtgatgat tagagactgt tgttatttac
    14641 caagacaaaa ttggctaaag agttgggtaa tgagctcatc aggagcagca tcccctgtga
    14701 tttctccgag aatttcccaa gttctggtta aatcaacctg tagtaaatcc actggcatgc
    14761 caagggctaa accatcattg acagcttcca aactttggac tgccttttca atcaaagaaa
    14821 tgtggcgggc atttgacagg taagtggcat cttgctctac caaaccagcg ttgtcaaaga
    14881 aaagctggtt gatgcgatct tcgatcaggt caatattatg gttggttaac accgaaatag
    14941 gaataaggtc cgctggcaac tgttccaatt caatcttttg ttccaaatct gttttattca
    15001 gcaatataat gcggttactg tcttgactga gatttaacaa agcccggtct tggtcggtca
    15061 gtttttcaga agcattcagc acaagaagaa caaggtcagc ttcttgaaga gcttttttag
    15121 accgttcaac tccgatttgt tcgaccagat catctgtctc acgaataccg gccgtatcga
    15181 caagttttag aggaactccc ttgatattaa cgtattcttc aatgacatct cgggtggttc
    15241 ctgcaatatc agtcacaata gccttgtctt ctcgcaacaa attattaagt aagcttgact
    15301 taccgacgtt tggacgacca ataatagctg ttgataagcc ctcacgcaga attttaccgc
    15361 gtttagctgt tcgaaggaga ttttccaaaa ggctttggaa ttcttgtgtt ttctcacgta
    15421 gcagagcagt ggtcatttct tccacatcgt cgtattcagg gtagtcaata ttgacttcga
    15481 cttgagctaa ggtgttgaga atttcctgac gggtgtcatt gataagctgg gaaagggagc
    15541 catccagttg cttgactgca atcgtcatag ccttgtctgt tttggcacga ataatgtcca
    15601 tgacagcctc agcttgagtc aaatcaacac gaccatttaa gaaggcacgt ttggtgaatt
    15661 cgccaggctc tgccattcta gcgccttggc gaatcaatag ttgcaagatt tcatttgtca
    15721 cagcaatacc accgtgcgta ttgatttcaa ccacattttc acgggtaaag gttttggggg
    15781 ctagcatgac tgacaccata acttcatcga taatagttcc tgtcttagga tcaatgatat
    15841 ggccatagtt aatagtgtgt gaggcgactt gctctaagtt ctttccctta aaaacagatt
    15901 gtgcaatggc aagcgcatct gtccctgata aacgaacaat gccaatagct ccttcaccaa
    15961 gaggggttga aatggcggta attgtatcaa attctttagt tatactcatg ccatctattt
    16021 taaatcaagt cctagtagaa agcaaggtat tattttgcat aga
//
LOCUS       DAUGFP010000035        15028 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.035, whole genome shotgun sequence.
ACCESSION   DAUGFP010000035 DAUGFP010000000
VERSION     DAUGFP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.035"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            5..78
                     /locus_tag="VH663_001199"
     tRNA            5..78
                     /locus_tag="VH663_001199"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            158..231
                     /locus_tag="VH663_001200"
     tRNA            158..231
                     /locus_tag="VH663_001200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:192..194,aa:Arg,seq:acg)
     gene            227..808
                     /locus_tag="VH663_001201"
     CDS             227..808
                     /locus_tag="VH663_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ8174402.1"
                     /translation="MHNEKNNRTLVSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKV
                     KPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYL
                     KDLLRRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQER
                     MQFQALIRGERFKGRRALLRKISPYFKEFAQQL"
     gene            995..1744
                     /gene="truA"
                     /locus_tag="VH663_001202"
     CDS             995..1744
                     /gene="truA"
                     /locus_tag="VH663_001202"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEQ8174403.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            1734..2498
                     /locus_tag="VH663_001203"
     CDS             1734..2498
                     /locus_tag="VH663_001203"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEQ8174404.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHLGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIISLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            2488..2958
                     /locus_tag="VH663_001204"
     CDS             2488..2958
                     /locus_tag="VH663_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8174405.1"
                     /translation="MQNSKIRQMSLTGILTALIVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGLKRWLGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            2972..3532
                     /locus_tag="VH663_001205"
     CDS             2972..3532
                     /locus_tag="VH663_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEQ8174406.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERYVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQFMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(3597..4442)
                     /locus_tag="VH663_001206"
     CDS             complement(3597..4442)
                     /locus_tag="VH663_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEQ8174407.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFD
                     GTLHFIPNRSITVVSNKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIV
                     GKPNILGPQNNSNGQFTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYS
                     ITSKS"
     gene            4763..6046
                     /locus_tag="VH663_001207"
     CDS             4763..6046
                     /locus_tag="VH663_001207"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEQ8174408.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDTSKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
     gene            6263..6838
                     /locus_tag="VH663_001208"
     CDS             6263..6838
                     /locus_tag="VH663_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEQ8174409.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEVEEDEEPVL"
     gene            7095..8699
                     /locus_tag="VH663_001209"
     CDS             7095..8699
                     /locus_tag="VH663_001209"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEQ8174410.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            8867..9793
                     /locus_tag="VH663_001210"
     CDS             8867..9793
                     /locus_tag="VH663_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ8174411.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLINRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIRENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            9847..9932
                     /locus_tag="VH663_001211"
     tRNA            9847..9932
                     /locus_tag="VH663_001211"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9881..9883,aa:Leu,seq:aag)
     gene            10140..11021
                     /locus_tag="VH663_001212"
     CDS             10140..11021
                     /locus_tag="VH663_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEQ8174412.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            11403..11591
                     /locus_tag="VH663_001213"
     CDS             11403..11591
                     /locus_tag="VH663_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEQ8174413.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            11743..12108
                     /locus_tag="VH663_001214"
     CDS             11743..12108
                     /locus_tag="VH663_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ8174414.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            12108..13772
                     /locus_tag="VH663_001215"
     CDS             12108..13772
                     /locus_tag="VH663_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEQ8174415.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKTKA
                     EAKDFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSNPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            14009..14899
                     /locus_tag="VH663_001216"
     CDS             14009..14899
                     /locus_tag="VH663_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEQ8174416.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFAAKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
BASE COUNT         4890 a         2393 c         3281 g         4464 t
ORIGIN      
        1 tttatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt
       61 cgagtcctac tggcggagta gaaagaggta ggaagcgtcc tatctttttt tgcattttag
      121 agaagagtgt ggtaagatag aggcaatata tgcgttggca cccttagctc aactggatag
      181 agtacctgac tacgaatcag gcggttagag gttcgactcc tctagggtgc ataatgaaaa
      241 aaacaatagg acattagtgt cctgttgttt ttttgtggtt ctctttatac tagggatagt
      301 taaaaagaaa aggagactca aaatgtcgat agagacaaga gcagcgtttg aaaaagttaa
      361 gcccattatt ttgaaattaa agcgacacta ttatattcaa ttgtgggata gagatgactg
      421 gttacaagaa ggacatatta tcttattaca gttactagaa aggtacccag agttaattga
      481 ggaagaagag cgtttatatc gctattttaa aacaaaattt tcatcttatt tgaaagattt
      541 attacgccgt caagaaagtc aaaagcgtca gttccataag ttagcatatg aagagatagg
      601 ggaggttgca catgccattc catcgagagg gttatggcta gacgactatg tggcttatca
      661 agaggtaata gctagcttag agaaccaatt aaattcgcaa gagcgtatgc agtttcaagc
      721 acttatcagg ggcgaacgtt tcaagggaag acgtgctcta cttaggaaga tcagtcccta
      781 ttttaaggaa tttgcacagc agttgtaagc aagacctata aggaattggg ggtaaatcta
      841 taaaaaaagg agagagagtt tggaggaaat agctttctta tagcttttaa atcgttagta
      901 gcttagagta agcttcaata cgtctttcat ttgtttgggt acagtttgat taaaaatagt
      961 ttctcttacg tttaagaaat gttaaaatag taacatggta agatacaaag caacgatttc
     1021 ctatgatggg actctttttt cgggttttca aagacaaaga catttacgta cggttcaaga
     1081 agagattgaa aaaacactct ataaattaaa taacggtaca aaaattataa ttcatggtgc
     1141 aggacgtacg gatgcaggag tacatgctta tggacaggtg atccattttg atttgcctca
     1201 agaacaagaa gttgaaaaat tacgatttgc tcttgataca cagactcctg aagatattga
     1261 tgttgttaat atagaaaaag tggctgatga ttttcattgt cgttatcaga agcatttaaa
     1321 aacttatgaa tttttagttg ataatggtcg acctaaaaat ccgatgatgc gacactatac
     1381 cactcattac ccttatactc ttaatattaa acttatgcaa gaagctatta acggtctggt
     1441 aggtacacac gattttactg gttttacagc agcaggaact tcggtacaaa ataaggtgcg
     1501 aacgattacc aaagcaacgg tatctcggga tgaaaaaact gattttttgg tgtttacttt
     1561 ttcaggaaat ggttttctct ataagcaggt gaggaatatg gtgggaactt tgctgaaaat
     1621 tggtaatggt caaatgccag ttgaacaagt taaagtgatt ttgagctcca aaaatcgtca
     1681 gctagctgga ccaacgatat ctggaaatgg gctttacttg aaggagattt gttatgaaaa
     1741 ctgattatat cgtgactata tctgggaacg atattttaag tggaggtggt ctctatgctg
     1801 atttagcgac ttacataagg tatgacctac aggcctttgt ggcagtaact tgtttaacta
     1861 ctagatctga agagggcttt agtctatttc cggtagctaa agaaattttc cgtgatcagt
     1921 tgaacagctt tacgaacgct ccaatttctg ctattaaaat tggtttgtta cctaatgcgg
     1981 aaatgtgtga aatagttttg gattttataa aggggcatct gggaatccca gtggttttag
     2041 atcctgttct agcatgtaaa gaaatagatg atgttaaaat agttccttta agacaggaga
     2101 ttttacaatt actgccttat gtaactgttg ttactccaaa tttggtagag gctcagctat
     2161 tatctcaaaa agagattatt tctttaaaag atatgcaaga agctgctaaa tatttttatc
     2221 aacttggggc aaaacaggtg gttatcaagg gtggtaatcg atttagtcag aaaaaggcta
     2281 ttgatctttt ttatgacggt aaagagattg ttaccttgga atgtcctgta cttgagaaaa
     2341 ataatattgg tgcaggctgt acttttgcat ctagtatcgc tagtcagtta gtaaagaaaa
     2401 aaacgccact agaagctgtg aaaaactcaa aagagttagt ctatcaagct attttacaat
     2461 ctgatagata cggagtaaaa caaagttatg caaaatagta agatacgaca aatgagttta
     2521 acagggatat tgacagcatt gattgttgtt ttaggaagat ttgtcatgct acctactcca
     2581 acaggttttt taacgctgtt ggatgcagga atttatgctg tcagcttttc ctttggatca
     2641 gctcaaggag ctattgtggg agggttatca ggttttttaa tcgatttagt ggcaggatac
     2701 cctcagtgga tgtttcatag tttgattgct cacagtgtcc aagggtattt tgcgggatgg
     2761 agagggctca agcgttggct tggtgttgtg ataggttctt ttatcatgat tttttggtac
     2821 tttttaggct ctcttatgct tggttatggc ctttcgggat ctttagccgg tatttgggga
     2881 aacgtcatgc aaaatacatt agggcttttt gttggtttta ttatctttaa ggccatatta
     2941 aggcaaaaaa agagataagg agagtgagaa gttgttgaat aatttagaga aacaaacaag
     3001 ggaaatcgtc attgatgttg tagaacgctc tgctattcaa ccaggtaatc tttttgttct
     3061 aggtctttca tctagcgaaa ttttgggtag tagaattgga aaacaatcta gtctagaagt
     3121 aggtcaaata gttgttgaag ttgtgttaga tgaattaaat aagcgtggtg ttcatttggc
     3181 tgtacaaggt tgtgaacacg tcaatcgtgc cttggtagta gagaggtatg tggcagaatc
     3241 taagcagtta gagattgtca atgtggttcc gaatcttcat gctggtggca gtgctcagat
     3301 ggctgctttt caatttatgt cagaccctgt cgaagtagaa gaagttattg cacacgctgg
     3361 acttgatata ggtgatactg ctataggaat gcatattaag cgtgttcaga tccctctgat
     3421 tccttgtcaa cgagagctag gaggtgctca tgtgacagct ttggctagtc gaccaaaatt
     3481 aattggtgga gctagggctg attacaatat ggatattatc cgtaaatcct aaaccctttt
     3541 ttctggaata aaacaattgg tgttttttct agaaaaaagg attttttaag attatattaa
     3601 gattttgagg tgatagaata gctgattgca gtcggtaatt gaatgccttc tttgagtaaa
     3661 gcttcttgat aaagtctata aaaagtatgg taaatcttaa attgttctcc attttctgtg
     3721 aaaatagcaa ttctaaaagt gaactgtcca ttagagttat tttgaggccc taaaatattt
     3781 ggtttgccaa cgatttgagg atggtttggc aattcctttt gattcacttc atcaatgatt
     3841 cgagtgactt ggctcaaatt aacagtggaa taaagaggaa tttcaattaa ggcacgcata
     3901 ttaccacgag atttgttgct gacaacggtg atgctacggt ttgggatgaa atgtagtgtt
     3961 ccatcgaatc cacgaatttg agtagttcta attccaacac caaagacact gccttctatg
     4021 tcagagatag taacgttatc tcctacttca aattgattct caaataagat aaaaaagcca
     4081 ttgacaacgt cagataaaaa tccctgtgca cctaacccga ttgctactcc agcaattcca
     4141 gctcctgcta aaagactaga gacaggtatg ccaaataagc taagtatcca ataaatgaga
     4201 agaaaatata ataagtaatt taaaatgttg tgagtgagct tagagagtgt ttttttgcga
     4261 gcttcacttt gtcttgaata agcaaacgat ttgttaatgg ttttttcaaa aagatagtta
     4321 gtaacacgtt ttaaaatagc aaaaaagagt aatagaataa tgagagacac tagcttttta
     4381 aaaatcgtta agccaatatt ttcgatatga gactgctcta ggtaatgtaa tataaatgtc
     4441 atgagaaatc ctttctttga tagtttatca aaaaaaaacc aatcttgaaa agagataaat
     4501 taatatttta accaaagtta tgataattgt tttatcataa agaatgccat agtcatccgt
     4561 tgtgataatg cttgaaaata gtcagttagt aagcgaaaaa gtctaacatt aacctttata
     4621 aattgaaaat aactgttagt agactaatga caggtagata ctcttaatag cgcgtttgat
     4681 ttcacgcata aaaagcttta aaaacgcttt aaattatgat aaaatgaact gtatcgaaat
     4741 tattttaagg agaaatgact taatgtctac atcatttgaa aacaaagcta caaatcgtgg
     4801 cgtgattaca tttacaatca gtcaagataa aataaaacca gctcttgata aagcttttaa
     4861 taaaatcaaa aaagacttga atgcaccagg tttccgtaaa ggacacatgc ctcgtccagt
     4921 cttcaaccaa aaatttggtg aagaagttct ttatgaagat gctttgaata ttgtattgcc
     4981 agaagcttat gaagcagctg tgacggaact tggtcttgat gtggttgcac aaccaaaaat
     5041 cgacgttgtg tcaatggaaa aagggaaaga gtggacactt tctgctgaag ttgtgacaaa
     5101 acctgaagtg aaacttggtg attacaaaaa ccttgttgta gaagttgata cttcaaaaga
     5161 agtttcagat gaagacgtgg atgctaaaat tgagcgcgaa cgtcaaaacc ttgcggaact
     5221 cattattaaa gacggtgaag cagctcaagg tgacactgtt gtgattgact ttgttggttc
     5281 agttgatggt gttgagtttg atggcggtaa aggagataac ttctctcttg aacttggttc
     5341 aggacaattt atcccaggtt ttgaagatca actagttggt gctaaagctg gcgatgaagt
     5401 agaagttaat gtcacattcc cagaatctta ccaagcagaa gatcttgcag gtaaagccgc
     5461 taaatttatg acaactattc acgaagtcaa aacaaaagaa gtaccagagc ttgatgatga
     5521 gcttgcaaaa gatattgatg aagatgttga cacacttgaa gacttaaaag taaaatatcg
     5581 taaagaactt gaagcagctc aagaaactgc ttatgatgat gctgttgaag gagctgcgat
     5641 tgaactagca gttgcaaatg ctgaaattgt tgatttaccc gaagaaatga ttcatgaaga
     5701 agtcaaccgt tcagtgaatg agtttatggg caacatgcaa cgtcaaggaa tctcacctga
     5761 aatgtacttc caattgactg gtacaactca agaagattta cataaccaat attctgctga
     5821 agctgacaaa cgtgttaaaa caaaccttgt tattgaagca attgctaaag cagaaggttt
     5881 tgaagcaaca gatagtgaaa ttgaacaaga aattaatgac cttgcaacag aatataacat
     5941 gccagctgac caagttcgtt ctcttctttc agcagatatg ttgaaacatg atattgcaat
     6001 gaaaaaagca gttgaagtga ttacaagcac agcaagcgtt aagtaatctt attaaggaac
     6061 aaaacgtaca agccggatta gaacgtcttt ctagtctggt ttttcttttt ctaaagaaat
     6121 aataaggaat tagttaaacg ttattttaat gaattagctt taaagatttg tcaagaagaa
     6181 gtgatatgag ttgataaaag ttttgacgaa ttactttttt tagcgtaaaa tggtagagaa
     6241 tgataaaata aggagaactg ccttgaaatt agacgtattt gcaggacaag aaaaaagcga
     6301 gctttccatg attgaagtgg cgcgtgccat tttggaagaa cgcggtcgcg acaatgagat
     6361 gtactttagc gatttagtca atgagattca aaattactta ggaaaatctg atgcaggtat
     6421 tcgtcatgca ttaccatttt tctatactga cttaaatacg gatggctcat ttattcctct
     6481 aggtgaaaac aaatggggac ttcgttcgtg gtatgctatt gatgaaattg atgaagaaat
     6541 tattactcta gaagaagatg aagatggcgc acaaaaacgt aagaaaaaac gcgttaatgc
     6601 tttcatggat ggagatgaag acgctattga ttatagagat gatgatccag aagatgaaga
     6661 tttcacagaa gaatcagctg aggttgaata tgatgaagaa gatccagatg atgaaaaatc
     6721 agaagtggag tcatatgatt cagaattgaa tgaaattatc cctgaagatg attttgaaga
     6781 agttgatatc aatgaagaag acgaagaagt tgaagaagat gaagaaccag ttctctaatg
     6841 tctttagttc agaaataaaa atgtaattat catttgacaa attgtctatt atagtttata
     6901 ttattattcg ggcacctctt tatgaggtca ggcaaaagct ctctagttaa tagggggcta
     6961 tttttgtttt ctagaataat gccagtttgc ctctatattt agaaaactaa ggtgatacct
     7021 aaccaatgtg gaaccaacta atagggaatt aacaagaaag caatgctttt attcagctta
     7081 tataaaggag tactatgacg aaatacattt ttgtaactgg tggtgttgtg tcatcaattg
     7141 gcaaaggaat tgttgcggca agtcttggcc gattgttaaa aaatcgtgga cttaaggtga
     7201 cgattcaaaa atttgatcct tatattaata ttgatccagg gacgatgagc ccctatcagc
     7261 atggagaagt atatgtgaca gatgacggtg ctgaaaccga cttggatttg ggtcactatg
     7321 aacgttttat tgatatcaat cttaataaat attccaatgt tacaacaggt aaaatttata
     7381 gtgaagtcct tcgtaaagaa cgtaaaggcg agtaccttgg agcgactgtt caggtgattc
     7441 ctcatatcac agatgccctt aaagaaaaaa tcaaacgcgc agcttcaaca acggactctg
     7501 atgtaattat tactgaggtc ggggggactg ttggcgatat tgaaagttta ccgttcttag
     7561 aagctcttcg tcagatgaaa gctgatgttg gttctgaaaa tgtgatgtat attcacacca
     7621 ccttgttgcc atatttaaag gcagcgggtg aaatgaaaac taagccaacg caacattccg
     7681 ttaaagaatt gcgtggttta gggattcaac cgaatatgtt ggttattcgt acggaagagc
     7741 cagttgagca ggggattaaa aataaattgg ctcagttctg tgatgtgaat tcagaagctg
     7801 ttatcgagtc acgtgatgta gaacacttgt atcaaattcc attgaacttg caagctcaat
     7861 caatggatca gattgtttgt gatcatttga aattgaatgc gccacaagct gatatgaccg
     7921 agtggtcagc tatggttgac aaggtgatga atttaagaaa aacaacaaaa attgccttgg
     7981 ttggtaaata tgtggaattg ccagatgcct acttatctgt tgtggaagcc ttgaaacatt
     8041 ctggttatgc caatgatacc gcaattgacc taaaatgggt caatgctaac gatgtgacgg
     8101 tggataatgc agctgacttg ttgggagatg cggatgggat tattgtacca ggtggttttg
     8161 gtcaacgtgg tacagaaggc aagatccaag cgattcgcta tgcccgcgag aatgatgtgc
     8221 caatgcttgg tatctgtctt ggcatgcagt tgacttgtgt tgaatttgct cgccatgtcc
     8281 tcaatatgga aggtgcaaac tcttttgaat tagagccaag tactaaatac cccatcattg
     8341 atatcatgcg tgatcaaatt gatattgaag atatgggagg tacgcttcgt ctgggattgt
     8401 acccatgtaa attgaaacca ggttcaaaag cagcaatggc ttacaataat caagaggtgg
     8461 tgcaacgtcg tcatcgtcac cgttatgagt tcaacaacaa attccgtcca gaatttgaag
     8521 cagctggttt tgttttctca ggggtatcac ctgacaatcg cttagttgaa attgtagaat
     8581 tgaaagagaa aaaattcttt gtagcagctc aataccaccc agaattgcaa agccgtccaa
     8641 atcgtcctga ggagctatac accgcttttg ttacagctgc tattaaaaat agcaattagt
     8701 ggttttaagg gtaagtttgt ggcattctac ttggctggca gatactgata aagattacta
     8761 gctattaaag ggatcttttc tataaaaata gcattagaaa aagaaaaacg tgatattttt
     8821 aggttagcac tttctttttc agtcattttt ttgtaaaata cttggtatga aaactattcg
     8881 aatagcaaag tatttaggaa ttctattcct tttgataact cttattagcg taggtgctag
     8941 tttttatttc tttcatgttg cacaaataag agaagagaaa tcgtttatta ataataagaa
     9001 acggagtaca aataatccat tatacccagc tgaacagtct tttgacgctt taccttacga
     9061 aaaacgtcaa ctaataaatc gtgggttaaa acaagtgggg tggtacttac cagctgctca
     9121 aaaaacaaaa aagacagcta ttgttgttca tggttttacg aatgacaaag aagatatgaa
     9181 gccatatgcc atgctttttc atgatttggg ctataatgtc ttaatgccag acaatgaggc
     9241 ccatggggaa agtgaaggga acttgattgg ttatggctgg aatgaccgcc ttaatgtcat
     9301 ggcttggaca gaccaactga ttagggaaaa ccctgaaagc caaatcacac tctttggctt
     9361 atctatgggt gctgcaacag taatgatggc aagtggtgag cgattgcctg cgcaagtcac
     9421 ctccctcatc gaagattgcg gttataccag tgtttgggac gaattgaagt ttcaggccaa
     9481 ggctatgtac aacttgcctg cctttccttt actctatgaa gtctctgcct tatctaagat
     9541 tcgagcaggt tttagttacg gagaagcgag ctcagtgaaa cagctggcta aaaataaacg
     9601 tccaacttta tttatccatg gtgataagga tgattttgtt cctacaaaaa tggtttatga
     9661 caattataag gccacgaaag gtcctaagga aatcttgatt gttaaagggg caaaacacgc
     9721 caaatccttt gaaacaaacc cagaacaata ccagaaaaaa attgccgctt ttttgaaaaa
     9781 agttgagaaa taagtattga cagttaaact cacagttgct ataatgattt atgttactga
     9841 tgagtagcgg ggatggcgga attggcagac gcgcaggact aaggatcctg tgaccgcttt
     9901 aggtcgtgtg ggttcaagtc ccactctccg cataatgtaa gaggtgaatc attgattcgt
     9961 ctctttttgt ttggttaaat gaaggataag aaggcataga tttaccggag aaaacttttg
    10021 ataatctgct tttggaaaac gctttaagct tgtgaaagac tagaaattta tggaaaattg
    10081 tgctagaatg aacaatgtaa acggggagtt atcccgaaaa ataagaggag gccttacaaa
    10141 tggcaatcgt ttcagcagaa aaatttgtcc aagcagctcg tgaaaacgga tatgctgttg
    10201 gtggatttaa cacaaacaac ttagagtgga ctcaagcaat cttgcgtgca gcagaagcta
    10261 aacaagctcc agttcttatc caaacatcaa tgggtgcagc aaaatacatg ggtggttata
    10321 aagtatgtca atctcttatt actaaccttg ttgagtcaat gggtatcact gttcctgttg
    10381 ctatccacct tgatcatggt cactacgaag atgctcttga gtgtatcgaa gtaggttaca
    10441 cttcaatcat gtttgacggt tcacaccttc cagttgaaga aaaccttgct aaaactgcag
    10501 aagttgttaa aattgcacat gctaaaggcg tttcagttga agctgaagtt ggtacaatcg
    10561 gtggtgaaga agacggtatc atcggtaaag gtgagctcgc tccaatcgaa gatgctaaag
    10621 caatggttga aactggaatt gacttcttgg cagctggtat cggtaacatc cacggtccat
    10681 acccagaaaa ctgggaaggt cttgctcttg accacttaga aaaattaaca gcagctgtac
    10741 caggtttccc aatcgtattg cacggtggtt ctggtattcc tgacgatcaa atcaaagaag
    10801 caatccgtct tggtgttgct aaagttaacg ttaacactga aagtcaaatc gctttctcta
    10861 acgcaactcg tgaatttgct cgtaactacg aagctaacga agcagaatat gatggtaaaa
    10921 aattatttga cccacgtaaa ttcttggctc caggtatgaa agctgttcaa ggtgctgttg
    10981 aagaacgtat cgatgttttc ggatcagcta acaaagcata atttagagta ttaaataccc
    11041 taaagccctc ttgagggctt ttttagatac aattgacatc ttaaaatgga taccagtttc
    11101 tgtgaaacaa tctttgattg tgaacaaaca aagcataatt tagagtatta aaatttggac
    11161 cctgctatct atggtaaatc tttttgttat tgttaaagag gttaagcgga atgttaatat
    11221 gttttagcta aaaaatgtag tgaaaaatga gtacgtagac tattgtaata gtaattccgt
    11281 aaaattgttg aaaaagaaaa tgggaaatac cttgtcaaat taagcaccct atgatagaat
    11341 agataggtat gtggtgctag cacatccgta aacatattct acgtaggagg aaacagatca
    11401 aaatggctaa agtatgttat tttacaggac gtaaaaccgt ttctggaaac aaccgttcac
    11461 acgcgatgaa ccaaacgaaa cgtacagtta aaccaaacct tcaaaaagtt actatccttg
    11521 ttgatggtaa acctaaaaaa gtttgggctt ctgctcgtgc gcttaaatct ggtaaagtag
    11581 aacgcatcta atagaaataa gccttagggc tttttttatt agcttatttc tatccataag
    11641 ctagtcaggg taataaacgc tattgttaac tattctatct cttttacatt acttctaaaa
    11701 tatgataaaa taggatgata aaaacttttt gaggtagtaa ctatgactgt aaaaattaat
    11761 acaaaagatg gtctaatcga gctatccgac gatgtgattg caactgtcgt gggaggatca
    11821 gcaacggaaa tttttggtgt tgttggaatg gccagcaaaa gtgcaataaa agacaatttt
    11881 caatcactac ttcgcaaaga aaactacgct aagggtgttg tagtaaaatc aacagatctg
    11941 ggtatttctg ttgatgtcta cactgtgatg agttatggtg tgaaaatcag tgaagtctct
    12001 aaaaatatcc aagaacgcgt gaaatttaac cttgaaagtc agcttggtct cactgctgat
    12061 atggtcaatg tttacgtgca aaatattaag gttgtaggag aaaattagtg tcaaatatta
    12121 caacaagctt attccaagaa atggtccaag cggcagcgac tcgccttggc aaacaagcag
    12181 aatatgttaa ttccttaaat gtttttcctg ttccagatgg tgatacagga acaaacatga
    12241 gcatgactat ggacaatggt gccaaggaag ttgctgataa acctgcgtca actgtgggag
    12301 aagttggaca aatgttatca aaaggtcttt tgatgggagc gcgtggaaat tctggagtta
    12361 ttacttcaca actttttcgt ggttttggtc aaagtattaa gggaaaagat gagctgacag
    12421 gtaaagattt ggcacaagcc tttcaagtgg gtgtagaagt tgcctacaaa gcagtgatga
    12481 aacctgttga agggacaatc ttgacagtat ctcgtggagc agcaaccgct gccttaaaaa
    12541 aagcagatct tacagacgac gccgtggaag tcatgcaagc tgctttagac ggtgccaaag
    12601 gtgcccttgc taagacacct gatttattac cagtccttaa agaagttggt gtggttgatt
    12661 caggcggtca aggtcttgtc tttatctatg aaggtttcct atctgctttg aatggcgact
    12721 atgtgacttc agcagacttt aaagcgactc ctgctaacat gtctgaaatg attaatgctg
    12781 agcatcacaa atccgttgtt ggtcatgtgg ctactgaaga cattacctac ggttattgta
    12841 ctgaaatcat ggttgccctt aaacaaggcc caacctatgt gaaagaattc aactatgatg
    12901 agtttcaagg ctaccttagt ggccttggtg actcgctgtt agtggtcaat gatgatgaga
    12961 ttgttaaagt tcacgtccat actgaagacc ccggacttgt gatgcaagaa ggtcttaaat
    13021 atggttcgct catcaagatt aaagtggaca atatgcgtaa ccagcatgaa gcacaggttc
    13081 aaaagactga tgtcgaaaaa accaaggctg aagcaaaaga ctttggcttg attgcagtag
    13141 tagctggaga gggtttatct gaaattttca aggcgcaagg tgttgattac gtgatttctg
    13201 gtggtcaaac catgaatcca tctacagaag atattgttaa agctattgaa gcagttaatg
    13261 ccaaacaagt cattatcttg ccaaacaata aaaatatctt tatggctgcc caatctgctg
    13321 cagaagtggt ggatatcccg gctgctgttg ttgcaacacg tactgttccg caaggattta
    13381 ccagcttgtt ggcctttgat ccttcaaaat ctttagagga caatgtagca gatatgtcaa
    13441 ctagtctttc tgacgtggtc agtggaagcg ttactttggc agtccgtgac accaccattg
    13501 atggtttaga aatccatgaa aatgatttct tgggaatggt tgatggtaaa atcatcgttt
    13561 caaacccaga catggaagca actctaaaag ctgcctttga aaagatgatt gacgaagata
    13621 gcgagattgt gactatcttt gttggtgaag aaggtgatca agacttagca gaagaacttg
    13681 ctgggtattt aggagagact tacgaagatg tggaagttga gatccatcaa ggagatcagc
    13741 ctgtttaccc atatctcatg agtgtagaat aatagaatac aataaaaaaa gaggagttag
    13801 gcaaaggtct gactcttttt gtctatattg atgtcgtaat gagtggtttt agtgatggta
    13861 agcccctaaa aaatgagggt aatccatcac aaagctaatg gttacgattt aatttgacaa
    13921 aacctagaaa aacattataa ttgtattata acaataagtc aagaactgaa aacggttcag
    13981 ttctaagata tgacaaggag gagattttat gttaggacca tttatcttta ttgcttttgg
    14041 ggttattgta atcctagcta ttgttgccag taccctttat gttgttcgtc aacaatctgt
    14101 tgccatcgtg gaacgttttg gtaggtacca aaaaacagcc acgagtggta ttcatgtccg
    14161 ccttccgttt gggattgaca aaattgctgc gcgtgtgcag ctgcgtcttt tacagtcaga
    14221 aatcatcgtt gaaaccaaaa ccaaggacaa tgtgtttgta actttgaatg ttgccactca
    14281 gtatcgtgtt aacgaacaaa atgtaacaga tgcttactac aaattaatga aacctgaatc
    14341 tcaaatcaag tcttatattg aagatgcctt gcggtcatcc gtgccgaagt tgaccttaga
    14401 cgaattgttt gagaaaaaag atgaaattgc cttggaagta caacatcaag tggcagaaga
    14461 aatgtcaacc tatggatata ttattgtcaa aaccttgatt accaaggtgg agcctgatgc
    14521 tgaagtcaaa cagtcaatga atgaaatcaa tgcggctcaa cgcaaacgtg tagctgctca
    14581 agagttggca aacgcagata aaattaaaat tgtgacagca gcagaagctg aggctgaaaa
    14641 agatcgattg cacggggtag ggattgccca acagcgtaag gccattgtgg atggtttggc
    14701 ggagtctatt caagagctta aagaagcgaa tatttcctta aacgaagaac aaatcatgtc
    14761 cattttgttg accaaccaat acctggatac cctcaatacc tttgcggcaa aaggaaacca
    14821 aacccttttt ctaccaaata caccaagtgg agtagaggat atccgtacac aagttttatc
    14881 tgcccttaaa acaaaataac gtgtaaaagc ttggctaaaa agttgctcaa tactggattt
    14941 agcagaaggc tatcataact tgtgaagtgg caaacgacga aatgaataca ttacttttat
    15001 tggagaaatg aaagtaaaaa aagccttt
//
LOCUS       DAUGFP010000036        15008 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.036, whole genome shotgun sequence.
ACCESSION   DAUGFP010000036 DAUGFP010000000
VERSION     DAUGFP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15008
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.036"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..638
                     /locus_tag="VH663_001217"
     CDS             <1..638
                     /locus_tag="VH663_001217"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEQ8174417.1"
                     /translation="RTVYKDQATMLNGARKETIDGKGALNNQISSLIFEKLNKAGVAT
                     HYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSKRFGVEEGRVLETPIVEFYYKNDD
                     LNDPFINDEHVKFLGIVNDEEIAYLKGETRRINELLKCWFAQIGLNLIDFKLEFGFDQ
                     EGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLGNLTDVYQVVLEKLIAL"
     gene            762..4487
                     /locus_tag="VH663_001218"
     CDS             762..4487
                     /locus_tag="VH663_001218"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEQ8174418.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKALRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDITEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFENYKADDFTAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHSGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRLSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGEIIVDLERRFLDTNGVRVVVDVKVVDKDLTV
                     PEARTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKDNFSQFEAIQAQHKIT
                     AASAAKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADAVKIG
                     QTQADFTVTVNGNDLAGASLLAAFEGKLEEVYPTEFEQTDVLEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEVAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQTLFASAVKYFTGK"
     gene            4648..6102
                     /locus_tag="VH663_001219"
     CDS             4648..6102
                     /locus_tag="VH663_001219"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEQ8174419.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPNFIGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCNRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     HLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            6130..7152
                     /locus_tag="VH663_001220"
     CDS             6130..7152
                     /locus_tag="VH663_001220"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEQ8174420.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGFASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVRAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     ASVVIK"
     gene            7320..7874
                     /gene="purN"
                     /locus_tag="VH663_001221"
     CDS             7320..7874
                     /gene="purN"
                     /locus_tag="VH663_001221"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEQ8174421.1"
                     /translation="MKIAVFASGNGSNFQVIAEHFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLAGYMKIVGETLLLAYERRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            8058..9605
                     /gene="purH"
                     /locus_tag="VH663_001222"
     CDS             8058..9605
                     /gene="purH"
                     /locus_tag="VH663_001222"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEQ8174422.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLDWDIISTGGTKVALDDAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDVDAAIRIIRDFKDRPTVVVLKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKIHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEAIALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(9663..10787)
                     /locus_tag="VH663_001223"
     CDS             complement(9663..10787)
                     /locus_tag="VH663_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ8174423.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNDNTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFIVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            11041..12306
                     /gene="purD"
                     /locus_tag="VH663_001224"
     CDS             11041..12306
                     /gene="purD"
                     /locus_tag="VH663_001224"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEQ8174424.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKIDGDIITYYAGAKFAENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDPTGLFYRNDIGSKAIRE"
     gene            12584..13075
                     /gene="purE"
                     /locus_tag="VH663_001225"
     CDS             12584..13075
                     /gene="purE"
                     /locus_tag="VH663_001225"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEQ8174425.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            13062..14135
                     /gene="purK"
                     /locus_tag="VH663_001226"
     CDS             13062..14135
                     /gene="purK"
                     /locus_tag="VH663_001226"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEQ8174426.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSACQLPQGTDLLRISQNR
                     IVEKDFLANKAGVTVAPYKVVTSSLDLGGLDLTKTYVLKTATGGYDGHGQKIIRSAED
                     LPEVQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKTAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVTENPSAHLHMYGKLE
                     AKHNRKMGHVTLFAEDADEVEEF"
     gene            14137..>15008
                     /locus_tag="VH663_001227"
     CDS             14137..>15008
                     /locus_tag="VH663_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174427.1"
                     /translation="MYLKYIFKIKDRNLVSDYESLVRNLTINLQKLVIKDRTALIGDK
                     QLPITIYYRGENEKFPTGIVKVESDSVEGLELFDKTIKEIVGNESRFGKFTCIYDGIS
                     TYYSTEIYSELHEIERRTQEFLREAFFLSDKSGELKEYQATIKKMDSSDFSFSKLINE
                     IFKASDITDTFIDHVRACVEFGTVPNGDLLPKSFWDSLDNSLLDDKEQGLEINNLLNE
                     IRSTRNTIAHCKEFKKKDYINCIEQVKKLKQALDKIIASIESTDISKMSREVSRINNS
                     LQADNDYIKQKYFI"
BASE COUNT         4331 a         2938 c         3500 g         4239 t
ORIGIN      
        1 ttagaacggt ctataaagac caagctacta tgttgaatgg agctcgtaag gagactattg
       61 atggcaaggg agcactaaat aatcagattt catctcttat ttttgagaaa ttaaataagg
      121 ctggtgtggc gacacattat attgagcaaa tttcaaaaaa cgaacaattg aataaaaaag
      181 ttgatattat tccgcttgag gtggtgttac ggaatgtgac agcaggctca ttttcaaaac
      241 gttttggagt ggaagaagga cgtgttcttg aaacgccaat tgtggaattt tactacaaaa
      301 atgatgatct gaatgatcct tttatcaatg atgagcatgt gaagtttttg ggtattgtta
      361 atgatgagga gattgcttat ctgaaaggag agactcgccg tattaatgaa ctcttgaaat
      421 gttggtttgc tcagattggg cttaacttga ttgacttcaa gttggagttc ggatttgatc
      481 aagaaggtac aattatctta gcagatgaat tttcgcctga taactgtcgc ctatgggata
      541 agaatggtaa ccatatggac aaggatgttt ttagaagaga ccttggcaac ctgacggatg
      601 tctatcaagt tgtacttgag aagttgatag cattgtaact agaagaggaa ggaattgaat
      661 ccgttcggga aactccgaaa aaaagatgga acttactgaa atctcaattt cagatgtcct
      721 cctattttcc agtcgctccc ttgtcggact tggtatctta tatgaataaa cgtatttttg
      781 ttgagaaaaa ggctgacttt ggtattaaat cggctagtct tgtgaaagag ttgacgcata
      841 atctacaact gacctctttg aaggctttgc gtattgtgca ggtctatgat gtcttcaatt
      901 tggctgagga tttgctggcg cgtgctgaga agcatatttt ctctgagcag gtgacagact
      961 gtcttttgac ggaaactgaa atcactgcgg agcttgataa ggttgccttc tttgccattg
     1021 aggcgcttcc tggtcaattt gaccaacgtg ctgctagttc gcaagaagct ttgctattat
     1081 ttggaagcga cagtcaggtt aaggtcaata cagcccagct ttacttggtc aataaggata
     1141 ttacagaagc agagcttgaa gccgttaaga actatctttt gaaccctgtt gattcgcgtt
     1201 tcaaggacat tactttgccg cttgaagagc aggctttctc tgtgtctgat aagacgatcc
     1261 ctaatcttga tttctttgaa aattataaag ctgacgattt tacggcctat aaggcagagc
     1321 agggcttggc tatggaggtc gatgaccttc tcttcatcca agattatttc aaatcaatcg
     1381 gacgtgtgcc aactgagact gagttgaaag ttttggatac ttactggtca gaccactgcc
     1441 gtcacacaac ctttgaaact gaattgaaga acattgattt ttcagcttct aaattccaaa
     1501 aacaattgca gacaacttat gacaaatata tcgccatgcg tgatgagctt ggtcgttctg
     1561 aaaagccaca aacacttatg gatatggcga ctatttttgg tcgttatgag cgtgccaacg
     1621 gtcgtctgga cgatatggaa gtctcagatg aaatcaatgc ctgctcagtt gagattgaag
     1681 tagatgttga tggtgtgaaa gagccttggc tcctcatgtt taagaacgag actcacaatc
     1741 acccaacaga aattgagcca ttcggtggag cggcgacttg tatcggtggt gctattcgtg
     1801 acccattgtc agggcgttca tacgtttatc aggctatgcg tatttcaggc gcaggcgata
     1861 tcacgactcc gattgcggaa acacgtgctg gtaaattgcc acaacaagtt atttctaaaa
     1921 ctgcggcgca cggctattct tcatatggta accaaattgg gcttgcgaca acttatgtgc
     1981 gtgagtactt ccactctggc tttgtagcca aacgtatgga gcttggagct gtggttggtg
     2041 ctgcacctaa ggaaaatgtg gttcgtgaaa aaccagaagc aggtgatgtg gtcatcttgc
     2101 tcggtggtaa aacaggtcgt gatggtgtcg gcggtgcgac aggttcatct aaggttcaaa
     2161 cggttgaatc tgtggaaaca gcaggagcag aggtacaaaa agggaatgcc atcgaagagc
     2221 gtaagattca acgtcttttc cgtgatggca atgtcactcg tctgatcaag aaatcaaatg
     2281 acttcggtgc aggtggtgtc tgtgttgcca tcggtgaatt ggctgacggt cttgaaatcg
     2341 atttggacaa ggtgcctctt aaataccaag gtcttaatgg tactgaaatt gcaatctcag
     2401 aatctcaaga gcgtatgtca gtcgttgttc gtctaagtga tgtggatgcc ttcatcgcag
     2461 cctgcaacaa ggaaaatatc gatgcagtcg ttgttgcgac cgttactgaa aaaccaaatc
     2521 ttgtcatgac ttggaatggc gaaatcatcg ttgatttgga acgccgtttc cttgatacca
     2581 atggtgtccg tgtcgttgtt gatgttaaag tcgttgacaa ggacttgaca gttccagaag
     2641 cacgtacaac atcagcagag acacttgaag cagatacgct taaggtcttg tctgacctca
     2701 accacgctag tcaaaaaggt ctccaaacca tctttgactc atctgttggt cgttcaaccg
     2761 ttaaccaccc aatcggtggt cgttaccaaa tcacaccgac agaaagttct gttcaaaaat
     2821 tgccagttca acatggtgtg acaacaactg catctgttat ggctcaaggt tacaatcctt
     2881 atattgcaga gtggtcacct tatcacggtg ctgcctatgc tgtcattgaa gcgacagctc
     2941 gcttggtagc aacgggtgct gactggtctc gtgcacgttt ctcttaccaa gagtactttg
     3001 agcgtatgga taaacaggca gagcgttttg gtcagccagt atcagctctt cttggttcta
     3061 ttgaggctca gattcaactt ggtttgccat caatcggcgg taaggactct atgtctggta
     3121 ctttcgaaga cttgacagta ccaccaacct tggtagcttt cggcgtgaca acagcggaca
     3181 gccgcaaggt tctctctcct gagtttaaag cggctggcga aaacatttac tatatcccag
     3241 gtcaagctat ttcagaagat attgattttg accttatcaa ggataacttt agccagtttg
     3301 aagctattca agctcaacat aagattacag ctgcctcagc cgctaaatac ggtggtgtcc
     3361 tagaaagtct tgctctcatg acttttggta accgtatcgg tgcttctgtt gaaattgcag
     3421 agcttgacag cagcttgaca gctcaactcg gaggttttgt ctttacatca gctgaggaaa
     3481 ttgctgacgc ggtgaaaatc ggtcaaactc aggcagactt tacagtcaca gtcaatggaa
     3541 atgaccttgc tggcgctagc cttctagcag ccttcgaagg caaattggaa gaggtttacc
     3601 caacagaatt tgaacagaca gatgttcttg aagaagttcc tgctgtggta tcagatactg
     3661 ttatcaaggc taaggaaaca attgaaaaac cagtggttta cattccagtc ttccctggta
     3721 ccaactcaga atacgattca gctaaggcct ttgaacaggt tggagctagt gtcaacttgg
     3781 taccatttgt aaccttgaat gaggttgcta ttgctgagtc agttgacact atggttgcta
     3841 atattgctaa ggcaaatatc atcttctttg ctggaggttt ctcagcagcg gatgaaccag
     3901 atgggtctgc taagtttatc gtcaatatct tgcttaacga gaaggtccgc gcagctattg
     3961 acagcttcat cgaaaaaggt ggccttatca tcggtatctg taatggtttc caagcccttg
     4021 ttaaatcagg tcttcttcca tacggaaact tcgaggaagc tggtgagaca agtccaactc
     4081 tcttctataa cgatgctaat cagcatgttg ccaagatggt tgagactcgt atcgcaaata
     4141 ccaactcacc ttggttggca ggagttgagg tcggcgatat tcatgccatt ccagtttcac
     4201 atggtgaagg taaatttgtt gtcagcgctt ctgaatttgc agagctaaga gacaatggtc
     4261 aaatctggag ccaatatgtg gactttgacg gacaaccatc tatggattct aaatacaatc
     4321 caaacggctc tgtcaatgcc atcgaaggga ttaccagcaa gaatggtcaa atcatcggta
     4381 agatgggaca ctcagaacgc tgggaagacg gactcttcca aaatatccct ggtaacaaag
     4441 accaaaccct ctttgcaagt gctgtaaaat actttacagg gaagtaaaaa ggttcagtga
     4501 acctttttag ccctcgcctt gaattagtga agggcgaggt tagaaagaat ctcgcttaat
     4561 ttttgagaaa tagtaaacac gggcgataag ttgtgcgaaa aactttctat tttcgcttta
     4621 cttgacagcc tttgtatcga ggtaaaaatg acatacgaag taaaatctct aaatgaagaa
     4681 tgtggagtct ttggtatctg gggacatcct caggcagctc aagtcactta ctttgggctt
     4741 catagccttc aacatcgcgg tcaagaagga gctggtattg tttcaaatga caatgggaaa
     4801 ctctatggtt atcgaaatgt tgggctcctt tctgaagttt ttaagaatca atctgaatta
     4861 gataatttaa ctgggaatgc ggctattgga catgttcggt atgctactgc aggttctgca
     4921 gatattcgca atattcagcc ttttctttat aaatttcatg acgggcaatt tgctttatgc
     4981 cataatggta atttgacaaa tgctatttcc ttaaggaaag aattagaaaa gcaaggtgca
     5041 attttcaatg cctcctcaga tactgaaatt ttaatgcatt tgattcgtcg aagccataac
     5101 ccaaatttca taggaaaggt aaaagaagct ctaaatacag taaagggtgg ctttgcctat
     5161 ctactgatga cagaaaataa actcattgct gctcttgacc ctaatgcctt tcgtcctttg
     5221 tcaattggac aaatgcaaaa tggtgcctgg gttatttcca gtgagacctg tgcttttgag
     5281 gtggtaggcg caaaatgggt tagagatgtt gaacctggcg aagttattct tatagacgat
     5341 agtggtattc aatgtaatcg ttatactgat gaaacgcaac tggcaatttg ttcgatggaa
     5401 tatgtctatt ttgcaaggcc ggattcaact atacatggtg ttaatgttca tacggctcga
     5461 aaaaatatgg gaaagcatct tgcacaagaa tttaaacagg atgctgatat tgtaattggt
     5521 gtcccaaatt cgtccttatc ggctgctatg ggctttgctg aagaatccgg attaccaaat
     5581 gagatgggtc ttgtaaaaaa tcagtatacg cagcgaacct ttattcaacc gacacaagaa
     5641 ttaagggaac aaggtgttcg aatgaaacta tcagcggtat ctggtgttgt caaaggaaag
     5701 cgcgttgtta tgattgatga ctcaattgta agaggaacga cttctagaag gattgtcgga
     5761 ttattaagag aagcaggagc tagtgaagta catgttgcta tagctagtcc agaattaaag
     5821 tatccttgtt tttatggtat tgatattcaa acaagacgag aattaatttc agcaaatcac
     5881 gctgctgacg aagtgtgtga tattattggc gcagatagtc tgacctatct ctcactagat
     5941 gggttgattg aatcaattgg actcgaaaca aaagcaccaa atggtggttt atgcgtagct
     6001 tactttgatg gacattatcc aacaccgctt tatgattatg aagaagaata cctcagaagt
     6061 ttagaagaaa aaacaagttt ttatattcaa aaagtgaaat aagctctgct aattatataa
     6121 ggagaaaaca tgtctgaaaa aaatgcttat gcaaaatctg gtgttgatgt tgaagctggt
     6181 tatgaagtgg ttgaaagaat aaaaaaacac gttgctcgta cagaacgtgc gggagtcatg
     6241 ggagctctag gtggctttgg tgggatgttt gacttgagta aaacaggagt tagagagcct
     6301 gttttggtct cagggacaga cggtgttggc acaaaactca tgcttgctat caagtacgac
     6361 aagcacgaca caatcggtca agactgtgtt gccatgtgtg tcaatgatat tattgcagca
     6421 ggtgctgagc ccctttactt ccttgactac gtagcgacag gaaaaaataa tccagtcaaa
     6481 tttgaagaag ttgtttctgg cgttgcagaa ggttgtgttc aagcaggagc agcactcatt
     6541 ggaggtgaga ccgctgaaat gcctgggatg tatggcgaag atgattatga ccttgctggc
     6601 tttgctgttg gtgtggctga aaaatctcag ctcatcgacg gttcaaaagt aaaggaaggc
     6661 gatattcttt tgggatttgc ttcaagtggt atccattcaa acggttattc tttggtacgt
     6721 cgtgtctttg ctgactatac tggtaaagag ctgcttccag aacttgaagg aaaacaactt
     6781 aaggatgtcc ttcttgagcc aactcgtatt tatgttagag cagctctgcc attaatcaag
     6841 gaagaactag tcaacggtat cggccacatc acaggtggtg gctttatcga gaatgttcct
     6901 cgtatgtttg cagatgattt ggctgcggaa atcgatgagg acaaggtgcc agtgcttccg
     6961 attttcaagg cgcttgaaaa atatggtgac atcaagcacg aagaaatgtt tgaaatcttc
     7021 aatatgggtg tcggtcttat gctagctgtt agtcctgaaa atgttaaccg cgtcaaagag
     7081 ctcttggatg aaccagttta tgaaattggt cgtatcatca agaaagcaga cgctagtgtg
     7141 gtgattaaat aagtcactaa attttataag aaaggagtgt ttagttagtt tgcctaactg
     7201 aacacgggat taaatttcta ggaaaaaaga taaaatgact tagacgcaag cgtcgttgtt
     7261 cgttttccta tttttctacg aaatttttcg gcaagccgaa tcatcccttt gtatcgaata
     7321 tgaaaatcgc tgtttttgct tctggtaatg gttccaactt tcaggtcata gcagagcact
     7381 ttccagttag ttttgtcttt tcagatcatc gtgatgccta tgttttagag cgtgctcaga
     7441 atttagccat tccaagcttc gcttttgaac tcaaggagtt tgagaataag gcggcttacg
     7501 agcaagccat cgttgatttg ttggacaaac acgagattga cttagtctgt ctggcgggct
     7561 atatgaagat tgttggagaa accttactct tggcctatga gaggcgtatt atcaatattc
     7621 acccagccta cctgcctgaa tttccaggtg cccacggtat cgaggatgct tgggaagctg
     7681 gtgttgacca gtctggcgtg accatccact gggttgactc tggtgtggat accggtcagg
     7741 tcatccaaca agtgcgcgtg ccacgcctag cagacgatag cctagaaagc tttgaaaccc
     7801 gtatccatga aaccgaatat caactctacc cagctgtctt agatagcttg ggagtggaga
     7861 ggaaagtaat ctagccttta tcttaaaatt ttaggtaaag ttaggaaaga tagtaaagaa
     7921 gaattaacgt tccaaaaaga aaggaattga acccgtctgg aaggcacaga aaaaagataa
     7981 acatctccta ggcgcaagcg tcttcgtcga gtttcctatt tttccttggc cttctagtcg
     8041 gacttggtat cttaaatatg actaaacgtg ctttaatctc agtgtctgac aagtcaggaa
     8101 ttgttgactt tgcaaaagaa ttgaaaaact tggattggga tattatctca actggtggga
     8161 ctaaggttgc ccttgacgat gctggtgttg agaccattgc catcgacgat gtgactggat
     8221 tcccagagat gatggacggt cgtgttaaga ccctccaccc aaacattcac ggtgggcttt
     8281 tggctcgtcg cgacgctgac agccaccttc aggctgctaa ggacaacaat atcgagctga
     8341 ttgacctcgt ggttgtcaac ctctatccct tcaaggagac tatccttcgt ccagacataa
     8401 cctatgattt ggcggtggaa aacatcgaca tcggcggtcc atcaatgctt cgctcagccg
     8461 ctaaaaatca cgctagcgtg accgttgtgg ttgacccagc ggattatgcg actgttttgg
     8521 gagaattggc tgacgctggt cagacgacat ttgaaacgcg tcaacgcttg gcagctaagg
     8581 tctttcgtca cacagcagcc tacgacgctt tgattgctga gtacttcaca actcaagtgg
     8641 gagaggctaa gcctgaaaaa ttaaccatca cttacgacct taaacaggct atgcgttacg
     8701 gagaaaatcc acaacaagac gctgatttct atcaaaaagc cttgccaaca gactactcaa
     8761 ttgcttctgc taaacagctc aacggaaaag aattgtcctt caataatatc cgtgatgttg
     8821 atgcagcaat ccgtattatc cgtgatttca aagaccgtcc gacagtggtt gtcctcaaac
     8881 acatgaaccc atgtggtatc ggacaggctg atgatattga gactgcttgg gattacgctt
     8941 atgaagctga cccagtttca atctttggtg gaattgtcgt ccttaaccgt gaagttgacg
     9001 cagcgacagc caagaagata caccctatct ttttggaaat catcatcgca ccatcatact
     9061 cagaagaagc gctagctatt ctcacaaata aaaagaaaaa cttgcgaatc cttgagttgc
     9121 cgtttgatgc ccaagctgcc agcgaagtgg aagctgagta cactggcgta gttggtggac
     9181 ttttggtgca aaaccaagac gttgtggctg aaaatccatc tgactggcaa gtggtgacag
     9241 accgccaacc aacagaacaa gaggcgattg cccttgagtt tgcctggaag gctatcaagt
     9301 atgttaagtc taacgggatt attattacta acgatcacat gacgcttgga ctcggtgcag
     9361 gtcaaaccaa ccgtgtcggc tcagtcaaga ttgctatcga gcaggctaaa gaccaccttg
     9421 acggtgccgt tctagcatca gatgccttct tcccatttgc ggacaacatt gaagaaatcg
     9481 ctgccgcagg gatcaaagca atcatccaac caggtggttc agttcgtgac caagactcta
     9541 ttgacgccgc aaacaaacat ggcttgacca tgatcttcac aggtgtgaga cattttaggc
     9601 attaataaaa aaatgtggtc taattaaaac cacatttttt attaaaagaa aatataagtt
     9661 aactataatt tgttagcttt atcagcattt acccatgttc cacgagatcc gatttgaaca
     9721 taaataccat tacttggttg atcaattttt aatactttat aactaccagt gacgataaaa
     9781 tattcaccca cacggagaat ttgatctcct aaaacttttc cttggttatc tgtttcatct
     9841 aatggtgtgg gatcaatcca gtttagtgga gttgggtctc ctccggctaa ttctttatta
     9901 acgatcaaat tattaacaag ctgatctaca cgaaaaacgc cagggaaggt aacagtatca
     9961 ccgaccttaa tggaaggttt tgtattgtca ttttgaacca cagactgtgc gggctcttta
    10021 atgggaatat agcgtcggtt tccagaaaaa ctgaggtagc taagccatgt ataacctcca
    10081 gcagtcacga ctttatcgta ataaacagat tgacctgctt catagtaagc aagatcaggg
    10141 ctatcgatac tggtttgtcc tttgactggt aatctggttg taaagtgata agttccagag
    10201 gggtcaaatg aagcttgaga aatgtgtttt agttgtcttg ggtaggaatg acttgtctga
    10261 gatgataagt ctttaaaatg gatataacca cttacctgag attttggaat ttgacgctta
    10321 tggtatcttt cagggccttg tccagcgttg taattatact cttcgatagt gacagtgtct
    10381 ccacggatat cagctaccca tgctacatga ccgtaagcag catttgactg ataagcgttt
    10441 ttatcaaacc aagcgatagc ccctatgctt ggtgtcttat tcacaggata accctgattt
    10501 ttcgcgatat gtccccacgt gcaggcatta ccgtagcctt taggtaactg aaaaccatta
    10561 gcagagctta aacgaaaagc tgcaaaagaa gtgcattggc ggatatacat gttccacgaa
    10621 tcgattccat tgcctttttt ccacttactc ggatagttat cgcctaaaac agctgcatga
    10681 acaagatttt cctgttgcga aataagtgta gatggcatag taggtactag accattaaaa
    10741 cctaaaagga ttactcctga gaccaaaaaa cgatgaaatt ttttcattaa ttacctcctg
    10801 taacttatta ttcgaaagca agaaacaaaa aatgttattt ttagatgatt attttgaaaa
    10861 ttggctttac ttgatattaa ataactgttt gattggctaa tagagtgttc cttatttcaa
    10921 gaattgtggt ctactatcac atttttacaa tgtttcaatt attgataact aatacattat
    10981 gtaaaaaaag cgaacgttta tgatataata tctaattata atacgaaaga ggtggatgtt
    11041 ttgaaattac ttgttgttgg ttcaggtggg cgtgaacatg cgattgctaa gaagttgtta
    11101 gcgtctaagg gtgtggatca ggtttttgtg gcacctggta atgatggtat gaccttggat
    11161 ggtctggact tggtgaatat agtagtttcc gaacattcta ggttgattgc ctttgctaag
    11221 gagaatgaga tttcttgggc ctttattggt cctgatgatg cgctagcagc tggtatcgtt
    11281 gatgatttca acagtgctgg actcagagct tttggtccaa caaaggcagc agcggagcta
    11341 gagtggtcaa aagactttgc caaggaaatc atggtcaaat acaatgtccc aacagcagcc
    11401 tatggcacat tttcagactt tgaaaaagcc aaagcctaca tcgaagagca gggcgcacct
    11461 atcgtggtca aggctgatgg cttggcgcta ggtaagggtg tggtcgtggc tgaaaccgtt
    11521 gagcaggcgg tagaggcggc gcaagagatg cttctggaca ataagtttgg cgactcagga
    11581 gcgcgtgtgg ttatcgagga attcttggat ggcgaggagt tttccctctt tgcctttgtt
    11641 aatggtgata agttctacat catgccgaca gctcaggatc acaagcgtgc ctttgacgga
    11701 gacaaggggc caaacactgg tggtatgggt gcctatgcgc ctgttcccca cctgcctcag
    11761 agcgtggtgg atacagcggt tgagaccatc gttaagcctg tccttgaagg tatgattgcc
    11821 gaagggcgtc cttatctagg cgtcctctat gcggggctta ttctgacggc tgatggccct
    11881 aaggttattg agttcaactc acgtttcggt gaccctgaaa ctcagattat cctccctcgc
    11941 ctgacttctg atttcgctca gaacatcgac gatatcatga tgggcattga gccttacatc
    12001 acttggcaga aggacggcgt gactctgggc gttgtcgttg cctcagaagg ctatccgctc
    12061 gattacgaga aaggtgtgcc actgcctgaa aagatcgacg gcgacatcat cacctactat
    12121 gctggagcta agtttgcgga aaatagcgag ctacttctct caaacggagg acgtgtctat
    12181 atgcttgtca ccacagaaga cagcgtcaaa gcagggcagg acaaaatcta tacccagcta
    12241 gcccaacaag acccaacagg tctcttctac cgaaatgaca tcggaagcaa agctataaga
    12301 gaataagact aaccattcaa atattagcgt cagcaatcaa cgaccgaagg tcgctccaaa
    12361 aagaaagaag aaaggaactg aacacgggac taatttccta ggaaaaaaat aaaactacct
    12421 ggacgcaagc gtcatcgtta gttttctgcg tcgtaggcgt ctgatgctaa agcacctagt
    12481 cgccctgacg tgggttcgtc ttcgaaatca aagatttcgg acttaccgca tatttttcta
    12541 cggaaatttt cgacaagtcg aaacgcccct ttgtatctta attatgaaaa ctccaattat
    12601 ttctattatc atgggctcca aatctgactg ggcaaccatg caaaaaacgg ctgaagttct
    12661 agacaacttt ggcattgctt acgagaagaa agtcgtctct gcccaccgta cgccagacct
    12721 catgttcaag catgctgagg aagcacgtgg tcgtggcatc aaaatcatca tcgcaggagc
    12781 aggtggggca gctcacttgc ctggtatggt ggcagctaaa acaaccctgc ctgttatcgg
    12841 tgtgcctgtc aaatcacgtg ccctttcagg tttggattca ctctattcga ttgtgcagat
    12901 gcctggcggt gtgcctgtgg caaccatggc tatcggagaa gcgggagcga ctaacgctgc
    12961 cctgacagcc ctccgtattc tctccatcga ggatcaaaat ctggctgatg cgctagctca
    13021 tttccatgag gaacaaggaa aaatcgcaga ggagtcaagt ggtgaactca tttaagacca
    13081 ttgggattat cggtggtggt cagctggggc agatgatggc cattgcggct atctatatgg
    13141 gccacaaggt catcacgctg gatcctgcta gcgactgccc tgcctcccgc gttagcgagg
    13201 taattgtggc gccttacgat gatgttgagg ctttgggaca attggctgcg cgttgcgatg
    13261 tattgactta tgagtttgag aatgttgatg cggacggttt ggatgccgtt gtgtcagctt
    13321 gtcagctacc gcaggggacc gatctgctcc gcatttccca aaaccgtatc gttgaaaaag
    13381 actttctggc aaataaggct ggtgtgacag tcgctcccta taaggtggtg acgtctagcc
    13441 ttgacctagg gggacttgat ttgaccaaga cctatgtcct caagacagcg acaggtggtt
    13501 atgacggtca tgggcaaaag attatccgct cagcagaaga tttgccagag gtgcagcaat
    13561 tagctaactc agctcagtgt gtcttggaag agtttgtcaa tttcgacctt gaaatctcag
    13621 ttatcgtgtc tggaaacgga gaggatgtga cggtctttcc cgttcaggaa aatatccacc
    13681 gcaacaatat cctgtcaaaa accatcgtac cagctcgcat ctcagatcaa ctggctgata
    13741 aggctaagaa aacggcggtg cagattgcca agaaactcca gctgtcagga actctctgtg
    13801 tggaaatgtt tgcgaccgca gatgatatca ttgtcaatga aattgcccca cgtccccaca
    13861 actcagggca ctactcaatc gaggcttgcg acttttcaca gtttgacacc cacattttgg
    13921 gcgtgctagg agcgccgctc ccaactatcc acctccatgc tccagccgtc atgctcaatg
    13981 tcctcggaca acacgtccag caggcgattg actatgtgac tgaaaaccct agcgcccacc
    14041 tccacatgta tggtaaacta gaagcgaaac ataaccgcaa aatgggacat gtgacactat
    14101 ttgcagaaga tgcggatgag gtggaggagt tttaggatgt atctaaagta tatatttaaa
    14161 ataaaggata ggaatttagt atcggattat gaatctttag ttcgaaattt gacaatcaat
    14221 cttcaaaaac tagttattaa agatagaact gcattaatag gggataaaca acttccaatt
    14281 acgatatatt atcgtgggga gaatgaaaag tttcctacgg gaatagtaaa agttgaatca
    14341 gactcagttg aaggattaga gttatttgat aaaacgataa aggaaatagt tgggaatgaa
    14401 agtagatttg gaaagttcac atgtatatac gatggaataa gtacatatta ttcaacagaa
    14461 atttattctg aacttcatga aattgagcgt cgaacacaag agtttttaag agaagcattt
    14521 tttctgagcg ataaaagtgg agaattgaaa gaatatcagg caactatcaa aaaaatggat
    14581 agtagtgatt tctcattcag caaattaatt aatgaaattt ttaaagcaag cgatataact
    14641 gatacattta ttgatcatgt tagagcgtgt gttgaatttg gaacagttcc aaatggagat
    14701 ttattaccaa aaagtttttg ggatagttta gataactctt tactagatga taaagagcaa
    14761 ggtttagaga ttaataattt attaaatgag attcgttcaa caagaaatac aatagctcac
    14821 tgtaaagagt ttaaaaagaa ggattatatt aattgtatag aacaagttaa aaaattaaag
    14881 caagcattgg ataaaataat agcctctata gagtctacag atatttctaa aatgtcacga
    14941 gaagtgtcac gcataaataa ctccttacaa gccgataatg actacatcaa acaaaaatat
    15001 tttataga
//
LOCUS       DAUGFP010000037        14836 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.037, whole genome shotgun sequence.
ACCESSION   DAUGFP010000037 DAUGFP010000000
VERSION     DAUGFP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14836
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.037"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            97..1260
                     /locus_tag="VH663_001228"
     CDS             97..1260
                     /locus_tag="VH663_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEQ8174428.1"
                     /translation="MNKLYIDSFVEKKLTAGVQLLDEKDFSNIKEKNQLVQLVTKSNR
                     PIGTAYISKQNKGIGWYLGPEKIDLSISYFVSLFSVAKAKRQDFAQSDETNAYRLFNQ
                     EGDGFGGVTIDLYKDFAVFSWYNAFVYDKKEMIMEAFQQVFPEVKGAYEKCRFKGPDT
                     ETAHLYGELAQETFSILENGIAYQVFLNEGLMTGIFLDQHDVRRALVDGLAMGKSLLN
                     LFSYTAAFSVAAAMGGAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYF
                     KYAKRKKLIFDVIVIDPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAA
                     NMTVSQFKKQIIKGFGSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            1257..1943
                     /locus_tag="VH663_001229"
     CDS             1257..1943
                     /locus_tag="VH663_001229"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEQ8174429.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVALNDMKRI
                     IEVLEMDISN"
     gene            2037..3203
                     /gene="aroC"
                     /locus_tag="VH663_001230"
     CDS             2037..3203
                     /gene="aroC"
                     /locus_tag="VH663_001230"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEQ8174430.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            3264..3605
                     /locus_tag="VH663_001231"
     CDS             3264..3605
                     /locus_tag="VH663_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEQ8174431.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADVSASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            3826..5178
                     /gene="gorA"
                     /locus_tag="VH663_001232"
     CDS             3826..5178
                     /gene="gorA"
                     /locus_tag="VH663_001232"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEQ8174432.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKEKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFSLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(5266..6534)
                     /locus_tag="VH663_001233"
     CDS             complement(5266..6534)
                     /locus_tag="VH663_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ8174433.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFP"
     gene            complement(6564..7004)
                     /locus_tag="VH663_001234"
     CDS             complement(6564..7004)
                     /locus_tag="VH663_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174434.1"
                     /translation="MSSRYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITITRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTSETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSTTEGTQLPNATGAEDANTGLPTNP"
     gene            7239..8381
                     /locus_tag="VH663_001235"
     CDS             7239..8381
                     /locus_tag="VH663_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ8174435.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPVRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     VYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            8393..9607
                     /gene="thiI"
                     /locus_tag="VH663_001236"
     CDS             8393..9607
                     /gene="thiI"
                     /locus_tag="VH663_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEQ8174436.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVR
                     SDRDRTHVYLNGTDYQPVVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQSFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            9645..10937
                     /locus_tag="VH663_001237"
     CDS             9645..10937
                     /locus_tag="VH663_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEQ8174437.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     ATVVALIVALLLFGLIYDLLGVQKNELAAQKSAQPKVKTARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    11042..11116
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            11151..11465
                     /gene="rplU"
                     /locus_tag="VH663_001238"
     CDS             11151..11465
                     /gene="rplU"
                     /locus_tag="VH663_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEQ8174438.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            11477..11803
                     /locus_tag="VH663_001239"
     CDS             11477..11803
                     /locus_tag="VH663_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEQ8174439.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            11831..12124
                     /gene="rpmA"
                     /locus_tag="VH663_001240"
     CDS             11831..12124
                     /gene="rpmA"
                     /locus_tag="VH663_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEQ8174440.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            12482..13396
                     /locus_tag="VH663_001241"
     CDS             12482..13396
                     /locus_tag="VH663_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ8174441.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLSDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            13393..13851
                     /locus_tag="VH663_001242"
     CDS             13393..13851
                     /locus_tag="VH663_001242"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEQ8174442.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMVHLDFVDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            13841..14731
                     /locus_tag="VH663_001243"
     CDS             13841..14731
                     /locus_tag="VH663_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ8174443.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDTLAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGYGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
BASE COUNT         4586 a         2577 c         3219 g         4454 t
ORIGIN      
        1 atctcagatt aaattatacc aaaaatgtga agctaatgct tgttgtaagt tcaaatttag
       61 taggattttt tatcagattt tgttataata aaaactatga ataaactcta tattgattct
      121 tttgtcgaaa agaagctgac agcaggggta caattattag atgaaaaaga tttttctaat
      181 ataaaggaaa aaaatcagtt ggttcaactg gtgaccaagt ccaatcgtcc cattggaaca
      241 gcatatatct ctaagcaaaa taaaggaatt ggttggtatc taggaccaga aaaaatcgac
      301 ttgtctatct cttattttgt tagtctattt tcagttgcta aggccaaaag acaagatttt
      361 gctcaatcgg acgagaccaa tgcttatcgc ctttttaatc aagaaggaga cggttttggt
      421 ggagtaacaa ttgatcttta taaagatttt gcagtctttt catggtacaa tgcctttgtc
      481 tatgacaaaa aagaaatgat tatggaggct tttcaacagg tttttcctga agtcaaagga
      541 gcttatgaaa agtgtcgctt taaaggtcca gatactgaaa ctgctcatct gtatggtgag
      601 ctggcacaag aaaccttttc gattttagaa aatggcatag cctatcaagt cttcttgaat
      661 gagggcttaa tgacaggtat ttttctagac cagcatgatg ttagaagagc actagtagat
      721 ggtttagcta tgggcaagtc ccttcttaac ctattttcct atacagctgc cttttctgta
      781 gcagctgcga tgggtggtgc tatcgagacc acttctgtgg acttggctaa acgatcgaga
      841 gaattatcac tagctcattt tgagcataac cagttaaact tagcctcaca tcattttgtg
      901 gtgatggatg tttttgaata cttcaaatat gccaaacgga aaaaattaat ctttgatgtg
      961 attgtgattg acccaccaag ctttgctaga aataaaaaac aaactttctc tgtgagcagg
     1021 gactaccaca aattaatcac agaggccttg gacatattga gtcctaaggg aactattata
     1081 gcttcaacaa atgctgctaa tatgactgtt agccagttca aaaagcaaat tatcaaagga
     1141 tttggaagtc gtcgtcctga gtctatgact ctccagcagc ttccgagtga ctttactatc
     1201 aataaagcag atgaaagaag taactattta aaggtattta ctataaaggt acgagaatga
     1261 gaattgtagc accagtcatg ccaagacatt ttgacgaggc tcaagccatt gatatttcta
     1321 aatatgaaga tgttaattta attgaatggc gagcagactt tcttcccaag gatgagattg
     1381 tcgcagtagc accagctatt tttgaaaaat ttgccggaaa agaaattatc tttacccttc
     1441 ggaccgttca agaagggggg aatattaccc tttcaagtca ggagtatgtt gatattatca
     1501 aagaaatcaa tgctatttat aatccagact atattgactt tgagtatttt acgcacaagt
     1561 cagtatttca agaaatgctt gattttccaa atcttatttt gtcttatcac aactttgaag
     1621 agactcctga aaatttaatg gaagcttttt cagaaatgac caagttggca ccgcgtgtgg
     1681 ttaaaattgc tgtgatgccc cagagtgaac aagatgttct agatctgatg aactatacca
     1741 gaggctttaa gacgctcaat cctgagcaag aatttgcaac tatatctatg gggaaattag
     1801 gaagattgtc tcgttttgcg ggagatgtca ttggctcttc ctggacatac gtttcattag
     1861 atcatgttag cggtccaggt caagtggctc tcaatgatat gaaacgtatt attgaggtct
     1921 tagaaatgga tatctctaac tagtgaatgg tctaaaaata ttatttctac aatagttaag
     1981 aaatgatgtt tttagattaa tgtttttaaa ataagtggaa ccaaaaagga gaaaaattgc
     2041 gttatttaac agcaggagaa tctcatgggc catccttaac agctattatt gaaggtatac
     2101 cagcaggatt aactcttcat ccagcggata ttgatcacga attacagcga cgtcaaggag
     2161 gttatggccg tggtgccaga atgtctattg agactgatcg ggttcaaata tcctcaggag
     2221 ttcgtcatgg caaaacaaca ggggccccca ttaccttaac ggtaattaat aaggaccacc
     2281 aaaaatggct agatgtcatg gctgtaggag acattgaaga gactctcaag ttaaagcgtc
     2341 gtgttaagca tcctagacca ggacatgcgg atttggtagg aggtattaaa tatcatttta
     2401 acgatttaag agatgcttta gagcgctcct cggctagaga aacaacgatg agagtggctg
     2461 taggcgctgt tgctaaacgt atcttagctg aacttggaat tgatatgtta catcatatct
     2521 taatatttgg tggtataacg atcacaatac cttctaagct ctcttttaga gagctgcaag
     2581 aaagggctct ccattcagaa ttatctattg ttaatccaaa gcaagaagaa gaaattaaaa
     2641 cgtatattga taagataaaa aaagaagggg ataccattgg tggtattatc gaaacgatag
     2701 ttcaaggggt gccagcaggt cttggatctt atgtccaatg ggataaaaaa ttagatgcca
     2761 agctggctca agcagttctt tccattaatg cttttaaagg ggtggaattt ggagttgggt
     2821 ttgatatggg attccaaaag ggctctcagg taatggatga aatcacctgg acacctaccc
     2881 agggctatgg tcgtcaaaca aaccacttag gtggctttga aggtggcatg accacaggac
     2941 aacctcttgt tgttaaaggg gttatgaagc ccattcctac tttgtataaa ccactcatgt
     3001 cggtagatat tgatagtcat gaaccttata aagcaacggt ggaacgctct gatccgacgg
     3061 ccttaccagc tgcaggggtt atcatggaaa atgtggtggc gacggtgctt gccaaagaga
     3121 ttttagagac tttttcttct actaccatgt ctgaattgca aaaagctttt tcggactatc
     3181 gtgcctacgt caagcaattt tagatagcga gatactggct tttatggtat catacaggta
     3241 ttaatgaaca aaaggagact gtcatgtcac aagaaattta tgactatgct aatcaacttg
     3301 aaagagcagt tcgtgctctg ccagaatacc aaaaagtgtt agaagtaaaa gaggccattc
     3361 aagcagacgt tagtgctagc gaactgtttg atgagtttgt cgctatgcaa gaaaaaatcc
     3421 aagggatgat gcaatcaggc caaatgccaa cggctgaaga acaaacaagc attcaagaac
     3481 ttagccaaaa gattgaagct aatgaccagc taaaagctta ttttgaagcg cagcaggcct
     3541 tatctgttta catgagtgat attgagcgta ttgtatttgc tccgttgaaa gatttagtca
     3601 agtaagccaa ttaagatagg aaaaaaacag attgctggca gctcgtcagg atctgttttt
     3661 tagtgtttat tttctttgta tcagaaagtt gagcctcttt cttatttgtg atatagtcaa
     3721 ataaagtgac caaaatagca ggccagtagt ggctttaatg aaggcaagtt gtctgctaac
     3781 tggctatgct aaaacatcag tattagaaag taaaaggtga taaaaatggt tattccttat
     3841 gattatattg ttattggtgg agggagtgca ggtattgctt ctgcgaacag ggcagccatg
     3901 catggggcca aagtattgtt agctgaaggt aaagagattg ggggaacctg tgttaaccta
     3961 ggctgcgttc ctaaaaaagt catgtggtat ggcgctcaag tagcagatat tttagggact
     4021 tacgctaagg attatggatt tgactttaaa gagaaagcat ttgattttaa acaattgaaa
     4081 gcaaatcgtc aagcttatat tgatcggatt catgcttctt atgagcgagg ctttgagcag
     4141 aatggtgttg accgtattta tgattatgct gttttcaaag atgctcatac ggtagaaatt
     4201 gcagggcagc tttataccgc accgcatatt ttgattgcca caggaggaca tcctgttttt
     4261 ccagatattg agggggcaca atacggtatc agctcggacg gcttttttgc cttagatgag
     4321 gtaccaaaac gtacggctgt ggttggggca ggctatattg ctgtggaact tgccggtgtt
     4381 ttgcatgctt tggggtcgaa gacggatttg tttatccgtc acgatcgacc tttaagaagt
     4441 tttgacaaag ccattgtgga tgttttagtt gaggaaatgg cagttggtgg tccttgctta
     4501 caccctcatt cagaagtggc aaaggttgtc aaaaatactg atgagtcctt gaccctttac
     4561 ctcaaagatg gccaagaagt tgaggttgac cagctcattt gggctatcgg tcgcaagcct
     4621 aatctagaag gttttagtct tgacaaaact ggagtgacgt taaatgataa aggttatatt
     4681 gagacagatg cctatgaaaa cacgtctgtc aaaggtattt atgccgtggg agatgttaat
     4741 gggaaactgg ctttaactcc agttgctgtg gcagctggac gtcgcctatc agagcgtctt
     4801 ttcaatggca aaacagacga gaaattggac taccagaacg ttgctactgt gatttttagt
     4861 caccctgtta ttggctctgt tggcttgtca gaagaagcag cagtcaaaca gtatgggcaa
     4921 gaagcagtta aaacatatca atcacgtttt acgtctatgt ttactgctat cactaaccat
     4981 cgccaacctt gtctgatgaa gttggtaaca gtcggagata ccgaaaaaat agttggtctt
     5041 cacggaatcg gatacggggt tgacgaaatg attcagggct ttgcagtagc catcaaaatg
     5101 ggagcaacca aggcggattt tgataacacc gttgctattc acccaactgg ctcagaagaa
     5161 tttgtcacca tgcgctaaaa aatgtagcct aaggcaataa aaaatgatgt taacagtttt
     5221 tgaggaaagt cttctaaact gctagcatcg ttttttgtta ggtgattagg ggaatatgtt
     5281 tttaaagata tttgcgttgc tcagacaata ttgccttacc tcggagataa aataaagtga
     5341 ccctgtaaca acaaagagtt catcagatgt tacggaacct tgtaaccagt ctctgtaatc
     5401 ttttacctgt gggtatttat caggataatc ctctaaaaga tttgcttcag ggaaatcaaa
     5461 agtcgtgaca gatacttcaa aagtatctag ttgagccagc aagtcggcta aaggcttacg
     5521 tttgagacct gcaaagagaa tatgaatagg acgtttggga aattcttctt gcagtagaga
     5581 ctttaattta gcaatggaat ctggattgtg agcgccgtct aatagaagat taggttgaat
     5641 aaattcggac cggccaggcc aagttgttgc ctcaattcct ttttgaatcg tttttggaga
     5701 tattttagga aaggattttc taagtaattg agctgtcata atggctaaac aggcattcgc
     5761 tttttgatgt tgacctaata gtttgagacg gagtttggtt aaattcaagt tatcgtgttg
     5821 aaaggaaaac gtcttttctg aaggattgag caaaaaatct tttcctaatt gatataagcg
     5881 tgaaccggct ttttgggtaa cttgttgaaa gacttgacta gcctcctgct caagttgacc
     5941 gataataacg ggttctttag ctttgataac ttctgctttt tggtgagcaa tttctgctag
     6001 gctataacct aaacgttctt ggtgatcaaa actaatggaa gggcatacga cggccagagc
     6061 atgaaaaaca ttggttgcgt cggttttccc accaatccct gcctcaataa tggcaatatc
     6121 aacaggttta agattggcaa agtagcaaaa cattatgaga gtcactaact caaactcggt
     6181 aggtctatcc caatttgttt caagggacat tttagtgaga atgggtctaa tcacgctcac
     6241 acattgcgcc aattctaggt ctgaaatggg ctggccatta aggcagatcc gatcgtgaaa
     6301 agagatgata aaaggagatg taaaggttcc gacctgataa ccagaagttg acaggatatg
     6361 ctgtaagtag gctgtcacag agcccttgcc gttagttcca acaacgtgaa tagcagggaa
     6421 tttgtcttgt ggccggccta gtgcctcaag taaccaagcc attctggcca aatcggtctt
     6481 gcgaccatta gccttaaagg tatgaatcca ggaaatgcta tcataggtag tcattactat
     6541 ttcttgtcct tctaacaatg cttctaaggg ttcgtaggaa gaccagtatt agcatcttca
     6601 gctccagttg cattaggaag ttgcgtccct tcagtggttg atggtgtctc aggggctgta
     6661 ggagttgtct cgttgtgaga aggtgtggta gaagatgacg gaagagttgt cttattatca
     6721 tctgtggttt ctgaagtacc tgtatggttt tgattaccct gagaagactc tttttcaata
     6781 atcttgacaa tggtattact cgattctttg ggtgtatcat tagaggtctt atctggtaca
     6841 ggctgcagtg ctcttgtaat ggtaatggtt gccacaatga tactcaaaat actaccaata
     6901 agtgcaatgg atttttggaa agctgtaaaa cctgttttaa tatgctcggt agggacacct
     6961 cctgatttac gaggtttttg ttgacgggaa taacgtgatg acatattttt ctcctttaaa
     7021 atcatcgtaa tgccaagatt aaggaggagg aaacagaagt cctagctcct ctttaacact
     7081 tcatctatca gtataatcta gaaaaaaagt gaagtcaacg gtttacctta tgtctaggac
     7141 aaggaagtcc tttgggctta ttgtaatacg aatattcttg atttcatttt agctattttg
     7201 tgatagaata aagtattgag taaaaatgaa ggtgacacat gatttatttt gataatgcag
     7261 ccacaaccat accttatgga gaggctctta aaacttacca agaagtagca acaaaaatat
     7321 atggcaatcc ctcaagtctg caccaattag ggacaaatgc gtctcgcatt ttagaagctt
     7381 cccgtaaaca gattgctggt ttgttgggtg ttaagtcaga agaaatcttt tttacgtctg
     7441 gtgggactga aagtgctaat tgggctatta agggaattgc ttttgaaaaa accgcttttg
     7501 gcaaacatat tattatctca gccattgaac accctgccgt gagtgaaagt gtcaaatggc
     7561 ttctcacaca aggattcgaa gtttcttatg ctccagtgac aacacaggga gttgttgatg
     7621 tgaatgcttt agctgagctg attagaccag acaccatctt aatctcgatt atggctgtta
     7681 ataatgaaat gggagctatc caaccggtta gagccatttc aaacctattg gctaaccaac
     7741 caacgatcac ctttcacgtt gatgctgttc aagccattgg taaaatacca ctctgtgatt
     7801 atatgactaa tcgtgtggat ttggcttctt tttcagggca caaatttcat agtgttcgtg
     7861 gtgttggctt cctttataaa aaagcaggca agcgactcaa tcctctcttg tcaggcggcg
     7921 gccaagagca ggagttacgt tcaaccactg aaaatgttgc aggaattgct agcatggcca
     7981 aagcattaag aatagtcact gaaaaacaag tgagcgtctt acctaaacta acagcaatgc
     8041 gagatgtgat ttacaaagct ttgtccgttt acccagatgt gacagtattt tcagctcaag
     8101 aaggctttgc gcccaatatt ttgacctttg ggattagagg ggttcgagga gaagttattg
     8161 tccacgcctt tgaaaaatat gagatttata tttcgaccac aagtgcatgt tcgtccaaag
     8221 caggtaagcc agcaggaagc ctagtggcta tggggattcc tgtaaaaact gcccagacag
     8281 ccgttcgtat tagtctagac gatgacaacg atatgggaca agtagagcaa tttttgacca
     8341 tattcaaaca gatttatgat aaaacacaga aagttaggta agattaggta agatggacta
     8401 ttcagaaatt atggttcgcc atggtgaact atcaactaaa gggaagaatc gcatgcgatt
     8461 cattaacaaa cttaaaaaca atattcaaga cgtactggct cctttcccag ccattactgt
     8521 ccgttcagat cgtgacagaa ctcatgtcta tttgaatggc acagattacc agccagttgt
     8581 agaagcttta aagctggtgt ttggtgttca agctctatct cctgtttata aacttgaaaa
     8641 aagtgtccct cttttggtaa cagctgttca agatatcatg acttctcttt atcgtgatgg
     8701 actgacgttt aagattgcca ctaagcgtag tgatcatgcg tttgaattgg atagtcgtga
     8761 gcttaacagt cttcttggtg gagctgtttt tgaggtttta ccaaatatcc aagcccagat
     8821 gaaacatccc gatgtgactc tcaaagtcga aattcgtgat gaagcagctt acatttctta
     8881 tgaagagatt aagggagcag gcgggttacc agtaggaacg tcagggaaag ggatgctgat
     8941 gttatcgggc ggtattgatt caccagtagc tgggtattta gcccttaaac gtggccttga
     9001 tattgaagtt gttcattttg ctagccctcc gtacaccagt ccaggtgcct tggctaaagc
     9061 ccaagacctt actcgtcggt taactcgttt tggtggaaat atccaattta tcgaggttcc
     9121 ttttacagaa attcaagaag aaattaaaaa taaggcacca gaggcttacc ttatgacatt
     9181 aacccgccgc tttatgatgc gcatcacaga tgccattcgt gagcaacgta agggtcttgt
     9241 tattgttaac ggtgagagcc taggtcaggt tgctagtcaa acccttgaaa gcatgcaggc
     9301 cattaatgcg gtaacatcga ccccaattat tagaccagtc gtgacaatgg ataaacttga
     9361 aattatcgag atggctcagg cgattgatac ttttgacatt tctattcaat cgttcgaaga
     9421 ttgctgtact atttttgcac cagaccgtcc taaaaccaat cctaaactag gtaatgctga
     9481 aaaatatgaa gaacgctttg atattgatgg tcttgtccag cgtgctgtgt caggaattgt
     9541 tgtcacagaa atcacacctg aaatcgtcaa tgatgaggtt gaaaatctca ttgatgcttt
     9601 actttaaaag aaaagatcaa gtgtaaatct tttagtagtc agtaatgata ctattaaaaa
     9661 gcctctataa gggctttttt gctgcaatga aggaggaaaa ttttgaggca agatgttata
     9721 atagagaaaa aggagggtgt gtcttgatta aaactatgca atacaaaaaa acgatggcaa
     9781 cagttgttgc tcttatcgtc gcacttttgc tatttggctt gatttatgat ttacttgggg
     9841 ttcaaaaaaa tgagctagca gctcaaaaat ctgctcagcc aaaggtcaaa acagcacgtg
     9901 ttgtagctaa tggcgacatt ttaattcatg atattctcta catgagtgct agaaaagctg
     9961 acgataccta tgatttcact ccttattttg aatatgttaa ggactggatt agcggtgctg
    10021 acttagctat tggagattat gaagggacca ttagcccaga ttatccctta gcaggctatc
    10081 ccttattcaa tgctccagaa gagattgcag gtgctctgaa aaatactggc tatgatgtag
    10141 tcgatttagc ccataatcat atcttagatt cccaattaga cggtgccctt aacactaaaa
    10201 aggtttttca tcaattaggt atagacagta ttggcatcta tgacaaggat cgttcaaaag
    10261 aatccttctt aattaaaaat gtcaatggta tcaaaattgc aattttaggt tattcttatg
    10321 gttataacgg catggaggcc acacttagcc aagaagacta tgagaaacac atgtctgatt
    10381 tagatgaagc taagataaaa aaagaacttc agctagctga aaaaaaggct gacgtgacca
    10441 ttgtcatgcc acaaatggga acagaatatg ccttagaacc gacagcagag caaaaagaac
    10501 tttaccacaa aatgattgat tggggagctg acgtcgtcct aggaggccat ccgcatgtta
    10561 ttgaaccttc agagacagtt ataaaaggta ggcaaaagaa attcattatt tactctatgg
    10621 gaaattttat ttcaaatcag cggcttgaaa ccgtagatga tatctggact gagcgtggcc
    10681 tattgatgga ccttactttt gagaaaaaag ataacaaaac aaaaattaaa acagttgagg
    10741 cccatccaac catggtttta gccaaaggta aaggcattgt gggtaaagaa ggttttgaac
    10801 tatacaatta tcgaacaatg gttttagaag attttatcaa aggtggaaaa taccacgaca
    10861 agattgatga ggaaaccaaa gcaaaagtag cacttgctta tcaggaaatt aatgatttag
    10921 ttaacctgaa atggtaatgt ttggtggttt agcatctaac gataaaaaat agagattaaa
    10981 cttaattttt ccttgataaa aagcagttat ggtgctaaaa tagtaacgtt gactaaatgc
    11041 acatcctgtg caaccgcgcg aaaatcgtta tcaagtagac caagtctcac gattccaggc
    11101 gagtcttcac agagggaaac ctcaaacaaa aattttatag gaggtgcatc atgagcacat
    11161 acgcaatcat caaaactggt ggaaaacaag ttaaagttga agtaggtcaa gcaatctacg
    11221 ttgagaaaat tgacgctgaa gctggcgcag aagtcacttt taacgaagtt gttcttgtag
    11281 gtggtgacaa aactgtagtt ggtactccag ttgttgaagg agctactgtc gttggaactg
    11341 ttgaaaaaca aggaaaacaa aagaaagttg taacattcaa atacaaacct aaaaaaggta
    11401 gccaccgtaa acaaggtcat cgtcaacctt acactaaagt tgtcatcaac gcaatcaacg
    11461 cttaatttac tagcttatga ttaaagcaat ttttactcgc caaaaaaatg gtcaactgag
    11521 cagtgttaca ctgacaggtc atgctggtag tggtaaacat ggctttgata ttgtttgtgc
    11581 ctctgttagt actctagcca ttaactttgt taattctctt gaggtgttag cagattgcca
    11641 agcattagta gatcttaatg atgttgaagg tgggtatatg gctattacaa taccaccaca
    11701 tgacaacaag gaggaagttc aattattatt tgaatctttt cttctaggaa tgactagttt
    11761 ggcgaaggat tcttcgaaat ttgtcaacac tcaggtcatc taaatttaat ttaagagagg
    11821 aaacaacatt atgttaaaaa tgaatcttgc taacttgcaa cttttcgccc acaaaaaagg
    11881 tggaggttct acatcaaacg gacgtgactc acaagctaaa cgccttggtg ctaaagctgc
    11941 tgacggtcaa actgtttcag gtggatcaat cctttaccgt caacgtggaa ctcatatcta
    12001 cccaggtgta aacgtaggcc gtggtggaga tgacacactt tttgctaaag ttgaaggtgt
    12061 tgtacgtttc gaacgtaaag gacgtgataa aaaacaagtt tctgtttacc cagtagctaa
    12121 ataattgttt aacatcgcat tatataaggc tttccaggtg tctggaaagc cgattttttt
    12181 attttggtac tcatgttgct acccatattt taaaaatcaa tgtaggaaga gagcttgtca
    12241 gcagttttat cttttgagga tttagtaaca tgagcgtaga tgttcatggt agtttggata
    12301 gtttcatgac caagtgtatt ggattttcat tgtttgtttc tatcctacta aagattgtag
    12361 ttgcttattt agagcaatag ttctaattgt aaaaagtcag cataataaaa caatgtcata
    12421 agatgttgtt ataacgggct ttatggtata attaaggata ctcattttag gaaggaaagc
    12481 aatgaatatt caacaattac gctatgtggt agccatcgct aataatggaa cttttcgaga
    12541 agctgcctct aaattgtttg tcagtcagcc tagcttatca gtttctatca aagatttaga
    12601 ggcggaatta gggtttcaaa tttttaatcg aactacttca ggaacagtct tgacaagtca
    12661 aggactagtt ttttatgaga aagctttaga agtagtcaaa tcttttgatt cttttgaaaa
    12721 aaccttttct caagctgatc tggatcaaaa tgaattttct attgctagcc aacattatga
    12781 ctttttgcca cctcttatta cagctttttc gcaacaatac gatggtcatc gtgtctttcg
    12841 tatctttgaa tcaacaacta ttcaaatttt agatgaggtt gctcaaggaa acagtgaaat
    12901 tggtattatt tacttgaatg tagacaatca aaaaggcctc tttcagcgga tggataagct
    12961 gggcttggaa tatgtttctc taattccttt tactactcat atttatcttt caaaaacgca
    13021 tccattggct aatcgcgaag cgctttattt gagcgatatt caaggattac ctgcggtaag
    13081 gtttacccaa gaaagggacg agtatttgta ttattctgaa aattttgtgg atactagtga
    13141 atgccctcgt atttataatg tgtcagatag ggcaaccttg aacggtattt tagagagaac
    13201 caatgccttt gcgacaggat caggttttct cgatcatcgg agtgttaatg ggattaaagt
    13261 gatacctcta gctgatcata ttgacaatca aatgatttat gtcaaacgaa aagataaaaa
    13321 cctttctgtt gctggggcca cttttgtgac tattttaaaa gactattttg ataaaagaag
    13381 gaaggcctta acatgaaaaa acgattgttt gtgcttagct tgatcctcct tgtagctttg
    13441 gatcaactta gtaaattttg gattgtttct catatagcgc ttggagaagt gaaacccttt
    13501 atcccaggta tcgtcagctt gacttacttg caaaacaatg gggctgcctt ttccatattg
    13561 caggaccagc aatggttctt tgttgtcata acggttttag ttatcggtta tgctatttat
    13621 taccttgcta ctcatcccca tttaaatatc tggaaacaat tagctctctt gcttattatt
    13681 tctggtggaa tcgggaattt tattgatcgt ttgcgtttag cttacgtgat tgatatggtt
    13741 catttagact ttgtggattt tgccattttt aatgtggcag attcatacct taccgttggt
    13801 gtcatattat tagtgatatg tttatggaaa gaagaggatt atggaaatta atgtgataac
    13861 atcggggcaa cgcttggata aggcactagc tgatttaagt cccctgtcac gtggtcaagc
    13921 caatgaccaa attaagcaag gccttgtttt agttaatggt cagcaaaaaa aggctaaata
    13981 tacggttcaa gcaggagatg tgatttgttt tgaactccct aaagaagaag tcttagagta
    14041 ccaagctcag aatattccct tggacattat ctatgaagat gatactctcg ccattattaa
    14101 caagccacag ggaatggtag ttcacccttc ggcgggacac ccttcaggaa caatggttaa
    14161 tgccttgatg tatcatatca aggatttgtc ctcaatcaat ggtgtggttc gtccaggaat
    14221 tgttcatcgg attgataagg acacctcagg tctcttaatg gtagctaaaa cagatgcagc
    14281 tcatcaagcc ttggcagaag aattaaaggc taaaaaatct ctcagaaaat acctcgccat
    14341 tgtccatgga aacctaccga atgatcgcgg gatgattgaa gctcctatag gtcgtagcga
    14401 aaaggaccgt aaaaaacagg ctgttacagc aaaaggcaaa gaagcagtga cacgttttac
    14461 agtgctagaa cgttttggcg attacagtct agtggaatta caactggaaa ctggtcgaac
    14521 gcaccaaatt cgtgttcata tggcctatat tggtcatcct gttgctgggg accctttata
    14581 tggaccacgt aagacactat caggttatgg ccaatttctt catgcaaaaa cattgggatt
    14641 aacgcatcct atgacaggaa aagaaatgat atttactgtt gaagctcccg aaatttttca
    14701 aaaggtgtta aagctgctaa gaaaaaaata aacagcgagg agtagccatt atttttagtc
    14761 ttatatcttt gtgtaaagtt tttaacatta taaaaaatac taaaaagcat ttacgataag
    14821 ccaggaaata tgagaa
//
LOCUS       DAUGFP010000038        14248 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.038, whole genome shotgun sequence.
ACCESSION   DAUGFP010000038 DAUGFP010000000
VERSION     DAUGFP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14248
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.038"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            189..710
                     /gene="pyrR"
                     /locus_tag="VH663_001244"
     CDS             189..710
                     /gene="pyrR"
                     /locus_tag="VH663_001244"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEQ8174444.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            726..1985
                     /locus_tag="VH663_001245"
     CDS             726..1985
                     /locus_tag="VH663_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEQ8174445.1"
                     /translation="MKDIIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKAIGNDWIDNILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            2046..2981
                     /locus_tag="VH663_001246"
     CDS             2046..2981
                     /locus_tag="VH663_001246"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEQ8174446.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            3025..4107
                     /gene="carA"
                     /locus_tag="VH663_001247"
     CDS             3025..4107
                     /gene="carA"
                     /locus_tag="VH663_001247"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEQ8174447.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            4353..7529
                     /gene="carB"
                     /locus_tag="VH663_001248"
     CDS             4353..7529
                     /gene="carB"
                     /locus_tag="VH663_001248"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEQ8174448.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPALDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQELQMHVDHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLTIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESRCFNIEAI"
     gene            7704..8975
                     /locus_tag="VH663_001249"
     CDS             7704..8975
                     /locus_tag="VH663_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ8174449.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGVGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVTVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            8975..9685
                     /locus_tag="VH663_001250"
     CDS             8975..9685
                     /locus_tag="VH663_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174450.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            9697..10917
                     /locus_tag="VH663_001251"
     CDS             9697..10917
                     /locus_tag="VH663_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174451.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGKGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
     gene            11171..12904
                     /locus_tag="VH663_001252"
     CDS             11171..12904
                     /locus_tag="VH663_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEQ8174452.1"
                     /translation="MTFLSDLISLMTKIRLSWVIKAGIFQLLFVTIANIVLSEFFYFI
                     LDVTGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQEISFYL
                     FRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEFIVGELS
                     KITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKHVLLWMK
                     LFAINGLTLVVLSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTIFFKLCS
                     AMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDTSHSKTVIAHR
                     GLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVNKTIRNL
                     TLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVDLFLETY
                     HRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVIEDFSYR
                     SYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLKQDNSYF
                     SRLVRIISSLY"
     gene            13031..13303
                     /gene="rpsP"
                     /locus_tag="VH663_001253"
     CDS             13031..13303
                     /gene="rpsP"
                     /locus_tag="VH663_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEQ8174453.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            13313..13552
                     /locus_tag="VH663_001254"
     CDS             13313..13552
                     /locus_tag="VH663_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEQ8174454.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
BASE COUNT         4554 a         2257 c         2908 g         4529 t
ORIGIN      
        1 ttacttgttt ttcttttctt tttttgctat actgattgaa ataaaatatt cctttaaaac
       61 agtcctgtga ggctgtcaag gagacggatt aaaaacatgc actatgatac ttaattgtgc
      121 tatattttgc tgaatctcct tgtttccaag gagatctttt tatttgaatt tttagaggag
      181 gtttcaacat gaaaactaag gaaattgttg atgacgtgac catgaaacgc gccattaccc
      241 gtattactta tgaaattatt gaacgtaata aacaattaga caatgtggtt ttggcaggca
      301 tcaaaacgag aggtgttttt ctagctcgtc gcattcaaga aagactgcat caattagaag
      361 ggttggattt accaattggt gagttagaca tcaagccttt tagagatgac atgagagttg
      421 aggaagacac aactctgatg tcagtagata ttacaggcaa ggatgtgatt ttgattgatg
      481 atgtgcttta tactggtcgt accatccgcg cggccatcga taacttagtg agtttaggtc
      541 gaccagcccg tgttagtcta gctgttttgg tggatcgtgg ccatcgggaa ttaccgattc
      601 gcgcggatta cgttgggaaa aatattccaa caagcagtgt agaagaaatt gttgttgagg
      661 ttgtcgaagt tgatggccgt gatcgtgtta gcattattga cccaacataa gaaggaggtt
      721 ttactgtgaa agatatcatt tatgatgtgg aagaagtgcc aaaggcaggt atgttagttg
      781 gtttgtcctt tcagcattta tttgccatgt ttggggcgac ggttcttgtt cctattttag
      841 ttggaattga cccgtctgtg gccctcctat cgagtggttt aggaacactt gcacatttat
      901 cggtaaccaa atttaaaatt ccagcctaca tgggctcaag ttttgcttat atagcagcta
      961 tgcagttact gatgaagaca aatggtattg gtgctgtagc tcaaggggct atgacaggag
     1021 gtctcgttta cctgattgta gctttgattg ttaaagctat tggcaatgat tggattgata
     1081 acatcttacc accgattgtg gttggtccga ttgttatggt cattggtcta agcctagctt
     1141 ctacagctgt taatgatgtc atgctaaaaa atgggaacta taatctgact tatcttgtta
     1201 ttgggttagt caccttatta tcagttattt ttttcaatat ttatggtaag ggaattgttg
     1261 ctattgtacc gcttttatta gggctattag ttggttatgt tgtagccctt ttagtgggtg
     1321 ttctcaccgg tcaagaaatt gttgatttta ccaatgtggc tcaggccaaa tggtttagta
     1381 tcccatcagt ggaaattcct ttcttaacct acggggttaa attctaccca agtgccattt
     1441 taactatggc acccattgcc tttgttacaa tgacagaaca ttttggacac attatggtct
     1501 taaatagttt gacaaaaaga gattatttca aggatccagg acttgaaaag actttaactg
     1561 gtgatggttt tgcgcaaatc attgccggct tcttaggggc acctccggtc acttcttatg
     1621 gtgaaaacat cggtgtaatg gctttgaata aaatattctc ggtttatgtt attgcaggtg
     1681 ccgcagtgat tgccgctctc ctcagtttta tcggtaaggt atcagcctta attcaatcta
     1741 ttccaacacc agttattgga ggtatatcag ttgccttgtt tggtgtaata gcttctagcg
     1801 gtttgaaaat cttgattgaa tccaaagttg atatggataa taagaaaaac ttattgattg
     1861 ccagtgttat tttggtctct gggattggag gattgatgct tcaagtaaat ggtcttcaaa
     1921 tttcaggtgt cgctttctca acacttcttg gcatcatctt atatcaggta cttcctgaaa
     1981 aataggaaga attgttgtta gaccacttac atagtcatca gtcgttagtg ataaagggga
     2041 taatcatgtc agttgtaaat aatcgcgttg cacttaccaa tttagtttca atggaagcat
     2101 taaccaccga agaagtttta ggtcttatca accgtggttc agaatataag gctggtaagg
     2161 ttgttatttc ggatcatcaa aaggatcttg ttgccaattt attctttgaa aactccaccc
     2221 gaacccataa gtcttttgaa gtggctgaga agaaactcgg cttgacagtt cttgatttca
     2281 atgctgatgc aagtgcggtt aacaagggag aaagtttgta tgatactgtg ttaaccatga
     2341 gtgcacttgg aacagacatc tgtgtgattc gtcacccaga agatgattat tacaaggaat
     2401 tagtagaaag tccaactatt acagcttcca ttgtcaatgg aggagatggt tctggacaac
     2461 acccgagtca atgtttactt gacttattaa ccatctatga agaatttgga cgctttgaag
     2521 gcttaaagat tgcgatcgct ggtgacttaa ctcactctag ggtggcaaag tccaacatgc
     2581 aaatcctcaa aagattagga gcagagcttt atttctatgg acctgaagaa tggtattcag
     2641 aagcctttaa tgcttacggc acttatattg ctattgacca gattattaag gaattggatg
     2701 ttcttatgtt gctgcgtgta cagcacgaac gtcatgacgg ccatcagagt ttttcaaaag
     2761 aaggttacca tcaagcgttt ggattgacac aggaacgtta ccaacaatta aaagattctg
     2821 ctattattat gcatccagct ccagttaatc gggatgtgga aattgcagat agtttggtgg
     2881 aagctcctaa agcgagaatt gtatctcaga tggctaatgg tgtctttgtt cgcatggcta
     2941 ttattgaagc cattttgaat ggtagaaata aaaactcatg atgaaagatt aaagcatgtt
     3001 tagaaggtag gaaaaggtag gaaaatgaca aaacgattac ttattttaga agacggcact
     3061 atttttgaag gagaaccctt tggtgctgat attgatgtga ctggtgaaat tgtctttaat
     3121 acaggaatga caggctacca agaatccatc actgaccaat cctacaatgg gcaaatttta
     3181 acctttacct atcctttgat tggaaattat gggattaatc gagatgacta tgagtccatt
     3241 tcacccacct gtaaaggggt tgttgtgtct gaagtcagtc ggttggcaag taattggcgc
     3301 aagcaaatga cgctagatgc gttcttgaaa ataaaaggga ttccagggat ttcgggaatt
     3361 gatacgagag ctttgaccaa aattattcgg caacacggta ccatgaaagc tacgatggca
     3421 gatgatggag atagtatcca gcatttaaaa gatcaactgc gtgcaacagt cttaccgact
     3481 aacaccattg aacaagtgtc aactaaaaca gcttatcctg caccagggat cgggaaaaat
     3541 attgtattgg ttgattttgg tttaaaacat tctattttaa gagaattttc taaaagacaa
     3601 tgtaatatca ccgttgtccc tttcaacatc actgcagaag aagtgctcca actaaatccg
     3661 gatggcttga tgctatctaa tggcccagga aacccagaag atttgccaga ggctttagac
     3721 atgattcgtg gggtacaagg taagattcct atttttggta tttgtatggg acatcaactc
     3781 tttagtctag caaatggtgc caagacttgt aaaatgacct ttggtcatcg tggctttaac
     3841 catgccgtta gagaaattgc aacgggaaga attgatttta ccagtcaaaa ccatggttat
     3901 gccgttgaac gtagtagtct acctgatact ttaatggtta cgcatgaaga cattaacgat
     3961 aagacggtcg aaggagtgaa acatagagat tttccagctt tttcagtcca atttcaccct
     4021 gatgcggctc caggacctca tgatgccagc tacttatttg acgaattttt ggaaatgatt
     4081 gattcttgga gatgtacgag taaatagtaa atcagttgca atttcacgac tatatcacct
     4141 cttgttactc tatttattaa catttacagt tgatttagta ccctatcaaa aatattattt
     4201 aatgttgctc tgtgaggcaa caaatagaga aaggaaggca aggagaacac aagtaaataa
     4261 tttagtaaaa agttaggttt tcaaagagat aaagctctta ttgagaagtg ctgactttac
     4321 cgcttcttgt ggtattcttg gtatcattaa ttatgccaaa acgaaaagat attcaaaaaa
     4381 tcatggttat cgggtctggc ccgattatta ttggacaagc tgctgaattt gattatgcag
     4441 ggactcaggc ctgtttggct ctaaaagaag aaggctataa ggtcatactt gttaattcaa
     4501 atcctgcaac gattatgaca gataaagaaa tcgctgataa ggtatatatt gagcctttga
     4561 ctcttgaatt tgttaatcgt atcattcgaa aagaaaggcc agatgccata ctgccaacct
     4621 taggtggtca aacgggcctc aatatggcaa tggcattatc taaagctggt attcttgatg
     4681 atttagagat tgaattgtta ggtactaagt tatctgcgat tgatcaggca gaagacagag
     4741 accttttcaa acaattaatg caagaactag accaacccat tccagaatct acaattgtta
     4801 aaacagttga tgaagctgtt acatttgcta gagatatcgg ttatccagtc attgtgcgcc
     4861 cagcttttac tcttggtgga actggtggag gaatatgtag tagtgaagaa gaactttgtg
     4921 aaattactga aaacggccta aagttatcac cggtcacaca gtgtttgatt gaacgctcaa
     4981 ttgctggctt caaagaaatt gaatacgaag taatgcgaga tagtgctgat aacgcgctgg
     5041 ttgtttgtaa tatggaaaac tttgatcccg tagggattca cacaggagat tctattgtat
     5101 ttgcaccaac acagaccctt tctgatattg aaaatcaaat gttacgtgat gctagtttaa
     5161 aaataattcg agctctgaaa attgaaggtg gctgtaacgt ccagttagca ctagatcctt
     5221 atagtttcaa atattatgtt attgaagtaa atcctagagt gtctcgttcc tctgcccttg
     5281 cttcaaaagc aacaggttat cccattgcca aattggctgc aaagattgct gttggcctaa
     5341 cacttgatga aatgattaac cctatcacag gcactactta cgctatgttt gaacctgccc
     5401 ttgattacgt tgttgctaaa attccgcgtt ttccgtttga taaatttgaa catggtgaac
     5461 gccaactggg aactcaaatg aaagcgacag gtgaagtcat ggctattggc cgtaatttgg
     5521 aagagtcctt actaaaagcc tgtcgttctt tagaaattgg tgtttgccat aatgagatga
     5581 caagtttaag taacattagc gatgaagagt tagtgactaa agtgattaaa gctcaagatg
     5641 atcgactttt ttacttgtca gaagcaatac gacgaggtta tagtatcgaa gaacttgaga
     5701 gcttgaccaa gattgattta tttttcttgg ataaattact tcatattgtt gaaatagagc
     5761 aagaattaca aatgcatgtt gaccatttgg aaagcctcaa aaaagcaaag cgttatggat
     5821 tttcagatca aaaaattgca gaaatttggc aaaaggatga aagtgatatc agagcgatga
     5881 gacattctca tagcctgtat cctgtctata aaatggtaga tacttgtgca gctgaatttg
     5941 atgctaaaac cccttatttt tattcaacat atgagttgga aaatgaatct gttcagtcga
     6001 ataaagaatc aattttagtt ttaggttcag gccctattcg tattggtcaa ggggttgaat
     6061 ttgactacgc aacggttcat tctgttaaag ccatccaaaa agctggctat gaagccatta
     6121 tcatgaattc aaatccggag actgtatcta cagatttttc ggtatcagat aaattatatt
     6181 ttgaaccgtt aacctttgaa gacgtcatga atgtgataga tttagagcag cctaagggag
     6241 taatagttca gtttggagga caaactgcta ttaacttagc acaagcactt tctgaggcgg
     6301 gtgttacaat attaggaaca caagtagagg atttggatag ggctgaagac agagatttat
     6361 ttgaaaaggc gttgaaagag cttggaatcc cgcaaccaca aggtcaaacg gcaactaatg
     6421 aagaagaagc tttagaggca gctaaaaaaa ttggttttcc agtcttagtt agaccttctt
     6481 acgtacttgg tggccgggct atggaaattg ttgaaaacaa agaagatctt agagaatata
     6541 taagaacagc ggttaaagca agtcctgaac atcctatctt agtggactct tatattttcg
     6601 gtaaggagtg tgaagtggat gccatttcag atggaaaatc agtcttaatt cctggtatta
     6661 tggagcacat cgaaagagca ggtgttcatt caggagattc aatggcagtt tatccgccac
     6721 agcaattatc taagcaaatt caagaaacaa tagctgagta tactaaacgt ttaaccattg
     6781 ggttaaattg tattggtatg atgaatgttc aatttgttat taaaaatgaa caagtctatg
     6841 ttattgaagt gaatccacgc gctagtcgta ccgtgccttt cttatcaaaa gtaacaggta
     6901 ttccgatggc tcagattgct actaagttga ttttaggtca aacattaaag gatctaggat
     6961 atgaagatgg tctttatccc caaagtcaac tggtgcatat caaagcgcct gtgttttcat
     7021 ttacaaagtt ggcacaggtt gatagtcttt taggtcctga aatgaaatca actggagaag
     7081 taatgggttc ggatactagt cttgaaaaag cactttataa agcgtttgaa gccaataatt
     7141 ctcatttatc agaatttggt caaattgtct ttactatagc agatgatagt aaggcagaag
     7201 ctctaagtct agcaagacga tttaaggcga ttggctatca aattatggct actcaaggta
     7261 cagcagctta ctttgctgag caaggcttaa gcgcgtgttt agttggaaaa attggtgatg
     7321 ctgccaatga catcccgaca ctcgtgcgac atggtcacgt tcaggcaatt gttaatacag
     7381 ttggaattaa acgcacagct gataaagacg gacagatgat tagaagttca gctattgaac
     7441 aaggagttcc tttatttaca gctttagata ctgcaaaagc tatgttaacg gttttagaaa
     7501 gcagatgttt taacatcgaa gcgatataaa aatgattttt ttagattata atgacaaaag
     7561 tttcagtaag ctattaattt gtcatagcat ataagtcatt atcttgtggc ttgttttcga
     7621 ctaagggcta agaatttctt gacaacaata taaaaaggtc atatacttgt cttatacaaa
     7681 tagtacaaaa ggggaggata gaaatgtcta aaagaggaaa aattaaaata acaacgaaaa
     7741 caaagcttat tacagctagt gttataacgc tagtattaat tataactgga gtagtcttgt
     7801 ggaaacaaca acaaaatacg ctaacagctg atatcgctaa agaaccttac tctactgtta
     7861 gtgtaactga agggagtatt gcttcttcga ctttactatc aggtactgta aaggctttat
     7921 cagaggaata tatttatttt gatgctaata aaggaaatga tgcaactgtt acagttaaag
     7981 taggtgatca ggtaacgcag ggccagcaat tagttcaata taatacaaca acagctcagt
     8041 cagcttatga tactgctgtt aggagtctta acaagattgg ccgacaaatt aatcatctta
     8101 aaacatacgg agttcctgct gttagtacag aaactaataa agatgaagct accggtgaag
     8161 agacgacaac aacagttcaa ccatcagctc agcaaaatgc taattataaa cagcagctgc
     8221 aagatttaaa tgatgcttat gcagatgcac aagcagaagt aaataaagcg cagatagcgt
     8281 taaatgatac agtagttatc agtagtgtct ctggaactgt tgtggaagta aataatgata
     8341 ttgatccttc ttcaaagaac agtcaaacac ttgttcacgt agcaaccgaa ggacagcttc
     8401 aggtgaaagg aacattgaca gagtatgatt tagcaaacgt taaggttggt caatctgtaa
     8461 aaattaagtc taaagtttat tctaatcaag aatggactgg aaaaatatca tatgtttcaa
     8521 actatccaac tgagtctaat gcaggttcaa caacgccagc aggtagcact ggagtgggaa
     8581 gttctacagg agctgcctat gattacaaga ttgatattat aagtcctctt aaccagctta
     8641 aacaaggttt cactgtttct gttgaggttg ttaatgaagc taaacaggcc ttagttcctt
     8701 taacggctgt tattaagaaa gataaaaaac actatgtttg gacttatgat gatgctactg
     8761 gcaaagccaa aaaagtagag gtgacacttg gaaacgcaga tgcacaacaa caagaaattc
     8821 ataaaggagt aactgttggt gacattgtta ttgccaatcc agataaaaat atcaaaccgg
     8881 ataaaaaact agaaggggtt atttcaatag gtaccaacac aaaaccggaa aaagattctc
     8941 aatcaaagaa taaaaaatca ggggtggata aatagtgtcg catgaaaaaa aacaattaat
     9001 gcaattatca aatattgtga agagctatca aaatggggat caagttctta aagttttgaa
     9061 aggtattaat ttaacagtat atgagggtga atttttagct attatgggtc cgtctggttc
     9121 gggaaaatca acactaatga atattattgg tttattagat cgtccaacat ccggagatta
     9181 cacattacat aatactaaaa tagaaatcct taatgatcgt gaattagcaa aagtacgaaa
     9241 tgatgaaatc ggctttgtct ttcaacaatt ctttctgtta gctaaattaa ctgcattaca
     9301 aaatgttgag ttgcctctta tttatgctgg tgtaaacgtc tcaaaaagac gtgaacaggc
     9361 taagcaattt cttgaaaaag taggacttgg acgacgtatc aaacatctac cgtcagaact
     9421 gtcaggaggt caaaagcaaa gggtggctat cgcacgagct ttagtaaatg acccatctat
     9481 tattttagcg gatgaaccaa caggtgcact agatactaaa acaggtcagc aaattatgga
     9541 actcttgaca gagttaaaca aagaaggtaa aacaattatt atggttactc atgaaccaga
     9601 aattgctgat tttgcaactc ggaaaattat tattcgtgat ggtgatatca caacggatac
     9661 tacagctagt gttgttattg actaggagga aaaataatgg aaaattggaa attcgctctt
     9721 agctcaattt gggggcataa gatgcgctca atcctgacaa tgttgggaat tatcatcggt
     9781 gtagcagcag ttgtgattat tatggggttg ggaaatgcta tgaaaaatag tgtaacaagc
     9841 acattttcta gcaagcaaaa agatattcaa ctctattttc aagaaaaagg agaagaagaa
     9901 gacctgtatg ccggattgca cacgcatgaa aataatcacg aagtgaagcc agagtggtta
     9961 gaacaaattg ttaaagatat cgatggtatt gattcttatt actttaccaa ttctgctact
    10021 tctactattt cgtatgaaaa aaagaaagta gacaatgctt cgatcatagg ggtaagtaaa
    10081 gattatttta atataaaaaa ctatgatatc gtagctggtc gtacactaac agataatgat
    10141 tattctaatt tttcgcgtat cattctttta gatacagtat tagccgatga cctatttggc
    10201 aaaggtaatt ataagtcagc tttaaataaa gtggtaagtt tatctgataa agattattta
    10261 gttattggtg tttataaaac tgatcagacg ccagtttctt ttgatggatt atcaggcggc
    10321 gctgtcatgg cgaatactca ggtagcttca gagtttggca ccaaagaaat aggatctatc
    10381 tacattcatg tgaacgatat tcaaaacagt atgaacttag gaaatcaagc agctgatatg
    10441 ctaaccaaca tatcgcatat taaagatgga cagtacgctg tccctgataa tagcaaaatc
    10501 gttgaagaaa tcaatagtca gttcagtatc atgacaacag tcataggttc aattgcagcg
    10561 atctctcttt tagtgggagg aattggtgtt atgaatatta tgttggtatc tgtcacagag
    10621 cgaactagag aaattggttt acgtaaagca cttggtgcaa cacgcctgaa aattttatca
    10681 caatttttga ttgaatccgt tgttctaaca gttcttggag gcttgatagg attattatta
    10741 gctcaattaa gtgtaggggc tttaggaaat gcgatgacat taaaaggagc ttgtatttct
    10801 cttgatgtgg ccttaatagc agttttattt tctgcctcaa taggtgtctt ttttggaatg
    10861 ttgccagcta ataaagctag taagttagat ccaattgagg cattacgtta tgaatagaat
    10921 agttcgtatc aattaatcta tagacgctga ctagtttaac gtctgattat atctagtttt
    10981 tgtattagca cagtagttat atcattgaag tgttagtgca ttttttgagg tgataatatc
    11041 tagcaaagct atcattggta tgatgatgtg gatagcgtat aagtgataca tctcttattt
    11101 ctagctgaac ttcggttcgg ctaggaagtt gtcaggcaat gagttgttta aaataaagga
    11161 gattagttca atgacatttt tatctgattt gatatcatta atgacaaaaa tcagattatc
    11221 ttgggtaata aaggcgggta tttttcaatt attatttgta acgattgcta atattgtctt
    11281 atcagaattt ttctatttta tattagacgt tactggtcaa tatcatttag ataaagacaa
    11341 tgttgtgact tttttaaaaa atcctatagc acttgcttta ttaggtgcct atttattttt
    11401 attagctgct tttattcacc ttgagttttt tgctctatat cgaattattg cggatcaaga
    11461 aattagtttc tatcttttta gaaaacagtt ttcttattac ctaagggggc tttggaaaac
    11521 attttctggt taccaattat tacttttttt gctttatatc ctactgacta ttccagtctt
    11581 acatattggt ttatcttctg tgattactca aaagctttat cttccagaat ttattgttgg
    11641 ggaattatca aagataacta gcacaaagta cttgctttat ggcagtctta ttcttgtgtt
    11701 ttaccttaac ctaagattag tatatttttt accattgata gcaatcaacc atcgtacggt
    11761 tgctcaagca tggagagaga gttggcaaaa gactaaaaag aaacatgtat tgttatggat
    11821 gaaacttttt gcaatcaatg gtcttacgct tgtagtctta tcgctagcta tttccatgat
    11881 tcttattttt gttgatatgt ttaatcctaa ggggaataat attattgttc agctgggagc
    11941 tttgaccttt acatgggagc tcattttttt tactactatt ttttttaaac tctgttcagc
    12001 aatgatttta aaagaggcaa ttgaaccaca aaagcaatat gatgagccaa gaagaagtaa
    12061 taaggcatat gttgtaatct ttatcgtggt gacagtaggt tttgcttatc aatctcttga
    12121 acgtttaact ttttttgaca catctcactc taagacagtt atcgcgcata gaggacttgt
    12181 atcagcaggt gtagaaaatt cactggaagc ccttgaaggt gctaagaaag caggaagtga
    12241 ttatgtagaa ctggatctaa tcttgactaa ggataatcac tttgtggtgt ctcatgataa
    12301 tcgattgaag cgtttagctg gagtaaataa gacgattcgc aacttaacct taaaagaagt
    12361 tgaacatcta acgagtcatc aaggacattt ttcagggcgt tttgtttctt ttgacacttt
    12421 ttatcaaaag gctaagaagt tgaatatgcc attacttatt gaactcaagc caattggtac
    12481 agaacctgga aattatgtcg atttgttttt agaaacttat catcgacttg gtataagcaa
    12541 agataataaa gtcatgtctt tagatttaga agtaatagaa gctatcaaga aaaaaaatcc
    12601 atcaattacg actggttata tcataccaat tcaatttggc ttttttggag atgaatttgt
    12661 tgatttctat gtcattgaag acttttctta tcggtcttat ttgtcgtccc aagctttttg
    12721 gaataataaa gaaatttacg tttggactat taatgatccc aagcgcatag agcattatct
    12781 cttaaagcct attcagggaa ttattacaga tcaaccagct ttaactaatc aattgattaa
    12841 agacttaaaa caagataatt cttattttag tcgattagtc agaattatta gtagtcttta
    12901 ttaaaggttt aaagaaagtg tcaagaaaaa aacttgacac tttctttaaa ctgagttata
    12961 ctgtttaaca taggaattta gcgtagctgt atttcttata cattactaaa agaaaagaga
    13021 ctatttaaaa atggcagtaa aaatccgttt aactcgtatg ggttctaaga aaaaaccttt
    13081 ctaccgtatt aatgttgcag attcacgtgc accacgtgat ggtcgtttta tcgaaacagt
    13141 tggaacatac aacccattag tggctgaaaa tcaaattact attaaagagg atcgtgttct
    13201 tgagtggtta tctaaaggag cgcagccttc tgataccgtt cgtaacatct tgtctaaagc
    13261 tggtgttatg gctaagttcc acgaccaaaa attctctaaa taataacagc ctatggatac
    13321 cattgaaaat cttattatcg ctattgtgaa acctttgatt tcacaaccag ataatcttac
    13381 cattaaaatt gaagatactc ctgacttttt agagtatcat cttgacttgg atgctcaaga
    13441 catcggtcgt gttatcggca aaaaaggtcg aactattact gcgataagat cgattgtcta
    13501 ctcggttcca acactaggaa aaaaagttcg cttagttatt gatgagaaat aatcactaac
    13561 ttgagatgga ttgaaaagct tgttgactta aagtcaacaa gctttttgaa tagcaaaaat
    13621 cttaatattt ctgaaatgaa aagattagat ttttctgtct ttaactaaaa agaagttaaa
    13681 gaatggctta gctgattgcc tttataagag attatactgg tttttgatag ctgctgtcat
    13741 gtaaaatagc tacaattcat cttacgagat tgaccttagc attgccagtg cagtctttat
    13801 tttccaacct gctgtttgtt gtatgtttat agttccctaa aaaatgctct ctcagttttt
    13861 tggggggaat tcctaaaatt aagtatactt aattttagga atgtttatat tctttacatt
    13921 ctgatgaaaa tacaaattat cctaacatga tgtattgaaa ggggaaatag tttatgttag
    13981 tatactgatt aagtgaagct gtaatgagac ttatacgatc aaaaaagtta gaataacttt
    14041 ataaaacagt ggtgttgaag caagtttagc ttcttctcag aaatagatta tctataaaaa
    14101 atcccaaaag tagtttgttt ccagttaatg aacttctttt gggaaggctt ttttgctggt
    14161 cgccacctca cttgtatatc gggaaccttc acgctaagat gtataagttg aatcgtttgt
    14221 ctatggcttg atgaaaatta atcaaaag
//
LOCUS       DAUGFP010000039        13876 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.039, whole genome shotgun sequence.
ACCESSION   DAUGFP010000039 DAUGFP010000000
VERSION     DAUGFP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13876
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.039"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..746)
                     /locus_tag="VH663_001255"
     CDS             complement(<1..746)
                     /locus_tag="VH663_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEQ8174455.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAETSTLTP
                     QGRQEKIKKIKDHITQYPKINEDRFWDRNWFRNSFKDEDYLKAVQNFTEQALQEILDL
                     IEKGATKGDKGDAGPRGERGPQGPEGKQGKAGERGAQGPKGDRGEQGIQGKPGEKGDK
                     GETGERGEKGETGATGAQGPQGEAGKDGAPGEKGEKGDRGETGAQGPVGPQGEKGETG
                     EQGPAGPQGEAGKPGEQGPAGPQGEAGKPG"
     gene            1128..1610
                     /gene="nrdI"
                     /locus_tag="VH663_001256"
     CDS             1128..1610
                     /gene="nrdI"
                     /locus_tag="VH663_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ8174456.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNDTQTLPNS
                     "
     gene            complement(2004..2822)
                     /locus_tag="VH663_001257"
     CDS             complement(2004..2822)
                     /locus_tag="VH663_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEQ8174457.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPNIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMADETDYRTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLDLQDSHKVADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLDWR"
     gene            complement(2905..5091)
                     /locus_tag="VH663_001258"
     CDS             complement(2905..5091)
                     /locus_tag="VH663_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ8174458.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHDSAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGAFASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSILFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDHNTQATPTQVADSNSQVVQIINLLGGAGNIDDVDACMTRLRVTVKDP
                     AKVGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGALIPEVNMSQLT
                     SKPTPAKDFKHVTEDVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTHGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETTIVVAFTNTTEIQDVTLTSLGNQPAKTKVATVEL"
     gene            complement(5448..6197)
                     /locus_tag="VH663_001259"
     CDS             complement(5448..6197)
                     /locus_tag="VH663_001259"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEQ8174459.1"
                     /translation="MQQYFIKGKAEKEVTITDKDTIKHMFQVMRLADEAEVILVFDDG
                     VKYLAKVTNSMAHELEIIETLPDQVELPVEVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEQKAEFLKSLSS
                     FDHIFIAYEETAKAGQLATLAREVKEVKSGVKLLFIFGPEGGISPAEITQFEAAGAIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            complement(6197..7150)
                     /locus_tag="VH663_001260"
     CDS             complement(6197..7150)
                     /locus_tag="VH663_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEQ8174460.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIEQKD
                     RFGELYPDVEQSDMIAITAYYPSSTNLADVIATINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQILRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQENIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLVKKEGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMIQGEWNALVFKKTDDISGVIGG"
     gene            complement(7221..7691)
                     /locus_tag="VH663_001261"
     CDS             complement(7221..7691)
                     /locus_tag="VH663_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEQ8174461.1"
                     /translation="MAKYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKADFFLEWNAQAFEEGQQGLEEAASYPYPRY"
     gene            7892..9649
                     /gene="pabB"
                     /locus_tag="VH663_001262"
     CDS             7892..9649
                     /gene="pabB"
                     /locus_tag="VH663_001262"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEQ8174462.1"
                     /translation="MHRKTIIDFKELGQRYLFDEPLVELVAKSLDQVGPVIENVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDSITIP
                     NQWVSATQKEAYQKAIETIHREMQQGNTYQVNYTLQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDEFAVISASPELFFKQEGNQLTTRPMKGTTKRGVNTWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQIGSVCVDRLCEVERYSTVWQMTSTIVGDLKADCDL
                     IDILKALFPCGSITGAPKVSTMAIITSLEPKPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVQQKTVFLYRHKQIFDLKTTAKVEHQKIAFL
                     EQHLNRLKEAATYFAYPYDEKALQKQLSTYLENKDNAAYRLMIRLSKDGKISLSDQPL
                     EPLSADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQVFYNQAGQLLETSIGN
                     LFIQLGQTLYTPPVAVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLELTHLDALLAKSQA"
     gene            9682..10248
                     /locus_tag="VH663_001263"
     CDS             9682..10248
                     /locus_tag="VH663_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEQ8174463.1"
                     /translation="MILLIDNYDSFTYNLAQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LVFSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGTLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            10281..11549
                     /locus_tag="VH663_001264"
     CDS             10281..11549
                     /locus_tag="VH663_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEQ8174464.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDSSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLKTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLSD"
     gene            11638..11834
                     /gene="ssrS"
                     /locus_tag="VH663_001265"
     ncRNA           11638..11834
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VH663_001265"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            11835..11907
                     /locus_tag="VH663_001266"
     tRNA            11835..11907
                     /locus_tag="VH663_001266"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11868..11870,aa:Lys,seq:ctt)
     gene            12048..12488
                     /locus_tag="VH663_001267"
     CDS             12048..12488
                     /locus_tag="VH663_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8174465.1"
                     /translation="MKIIEYKGNYRDNLIFMVLEAKNALRRIPSINPDLLTIETSYLE
                     KGDCFWLAVNEESRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYL
                     RKIGKSHVRIHLGQPKEQWFESYRFYPKHGYQFYDDTHLIKKLI"
     gene            12543..13049
                     /locus_tag="VH663_001268"
     CDS             12543..13049
                     /locus_tag="VH663_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEQ8174466.1"
                     /translation="MKIAIIGHSGSGKSTLARFLGQHYHCEVVHLDQLHFSSNWQERS
                     DHDMIADLSTCLLNQDWIIEGNYANCLYEERMSEANHIIYVNFSRFHCLYRAFKRYLN
                     YRGKTRPDMADNCQEKFDVAFVKWILLDGRSRNQLKKYQSVIQKYPHKTIVLTNQKQL
                     NHYMNTIN"
     gene            13298..>13876
                     /locus_tag="VH663_001269"
     CDS             13298..>13876
                     /locus_tag="VH663_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ8174467.1"
                     /translation="MKKTKLIFSLTSIFIAIISRPVFGLEVDNNSLLRNIYSTIMYEY
                     SDTVIDFKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKAGDKIAVFSVPFDWNYL
                     SEGKVIAYTYGGITPYQKTSIPKNIPVNLWINGKQISVPYNEISTNKTTVTAQEIDLK
                     VRKFLIAQHQLYSSGSSYKSGKLVFHTNDNSDK"
BASE COUNT         3951 a         3186 c         2703 g         4036 t
ORIGIN      
        1 tcgcctggtt tgcctgcttc accttgtggg cctgctgggc cttgctcacc tggtttgcct
       61 gcctcacctt gtgggcctgc tgggccttgt tcgcccgttt cacctttttc accttgtggg
      121 cctactggac cttgagctcc ggtttcaccg cggtcacctt tttcaccctt ttcgcctgga
      181 gcgccatctt taccagcttc accctgtggg ccttgggcgc cagttgctcc ggtttcacct
      241 ttttcaccgc gctcaccggt ttcacctttg tcgccttttt caccaggttt accttggatt
      301 ccttgctcac cgcggtcacc tttaggacct tgggcgcccc tctcaccagc tttaccttgc
      361 ttgccttctg gaccttgtgg gccgcgctcg cctctcggac cagcatcacc tttatcgccc
      421 tttgttgcac ctttttcaat cagatctaga atttcttgga gtgcttgttc agtaaaattt
      481 tgtactgctt ttaaataatc ttcatcctta aaagagttac ggaaccagtt ccgatcccaa
      541 aatctgtcct catttatttt tggatattgc gtaatatggt ctttaatttt tttaattttt
      601 tcttgtctgc cttgtggcgt cagagtagaa gtctcagcct taacgctgct tgccccgcct
      661 ccaaaggcta gaaggacggc agcggctgag gttagcccgt agcgccagac cagtttgttg
      721 agattatggt gttttgatgt caacatatgt tgttctctct ttctctatta gatgatgtgt
      781 tttagataaa aataacatgg tagctagacc tgattattta taacagtatt ataatagagt
      841 gtgaaaacga ttgtaaagtt ctcttttgtt gttcatcttt tagattttga ctgattgctc
      901 ttttgtggag attagagggc cactttggca cttgcctgcc tcgtttttaa gtcgagggca
      961 agcggatcct ttgtttaagg tcactctttt ttaaaatgtt actttttttg actgcttgta
     1021 ccttcttgtc tggttggata ccgctaggtt tcttctttac ttgtttgttg gtcttgattg
     1081 tggtaagctt atgaagaaac cttgtaggat aaaaaaagga gacacttatg cctcagatta
     1141 cccttgtttt tattagcctc agtggcaaca cccttagctt tgtcaagcgg ctatcccttt
     1201 atctagcaga caattacgat taccatgtca aacagattaa cattaaagat ttgaaacacg
     1261 agacttttcc cgtcaaggaa gagtttgtgg cgattctacc cacttatcta gaaggcggaa
     1321 atggcgttga ctctggtgag gtggagatcc tcaccactcc tttgggagag tttattgctg
     1381 cccatggcaa cgcccagcgt tgtcttggga ttattggctc aggcaacaaa aactttaacc
     1441 atcagtattg cctgacagcc aaacagtatg ccaaacgctt tggctttcca ctgctaggtg
     1501 attttgagct gcgagggact cctgatgaca tttcgcggtt agcccaggtc atcatggagg
     1561 ccagctccag acactcgtct aacgacacac agacactgcc taacagctag acagtctata
     1621 gatgatcata gctgttttaa aaaagagcac ttttaggaca ccaactaggg aaggccccta
     1681 ccccacgcct tgtgatttct cagcctttta aaagagcgat cacagtgctt cttggggctt
     1741 gggatcacac caagcactcc tagaaatatc cccatgcaga ctttagcaag gcaactgtac
     1801 gcttggtggt actttctgtg aagctcagat ggttgggggc aaaaaagccc tggtttttta
     1861 gggttactga catgactaaa gaggtctaac cagctcaagg tagagctggt tagacctctt
     1921 tgtgttctcc taatccccct agacgatcgc agtttcttat gtgtcttggc ttctcctatg
     1981 tggtgtgcct gttagcacat gctctaacgc cagtctaatg tcacttctaa accatagtgg
     2041 tcactgacga ctggcgcatc cccgccttca aaggtaatct tagaagatcg gatgatgaag
     2101 tccttactgg taaagacatg atccaccttg agggcttctt tgttaccttg ccagccatca
     2161 atatcagcca cgatgctatg atcaccaaac acgtggtcag caaccttatg gctatcttgt
     2221 aggtctaggg ggctacccat catcacttga taaccgtctt gatccgttgg attgttaaaa
     2281 tctcccataa gtagcaaggg acagtttagc gtgagcagct ctttttctaa tttctcccac
     2341 tcccctaaaa accccttgtc aaaccatgac aagtgcacat tgacaacagc gacttctttg
     2401 ccatctaacg tggttttggc tatgagtgag cgtctggtac ggtagtctgt ttcatcagcc
     2461 atcgctgaca cgagaatatc tgaaacatga ataggttgtt ttgataagat tgccacgcct
     2521 tcctggtaaa tgtcatagcc aatatggtta taagcccaag accaataata gtgctgccct
     2581 cgtttttgta aataatggat caagagaaga gcaaaatggt ctttatgaat gtttggtgtc
     2641 cctgaaagag cttgatatct tggtaaatct gtggccagct cgctctcgat taactgattg
     2701 atttcttgca agcaaatgat atcgtatttt tcagctaaaa tatgctcagc caaagcaact
     2761 aattttttaa gagtgttagc ttgcatccac gaatgggtat tcagtgttaa taccttggtc
     2821 attaatcctc ctttataaga caagaagagc catagggcct aaaccagcta tggctattac
     2881 ctatcactat ctcgtgatgt tttgttaaag ctctacggtg gctactttag tcttagcagg
     2941 ttgattgcct aaagaagtca aggtcacatc ttggatctct gttgtgttgg taaaggctac
     3001 cacgatagtg gtctcacgct ctgctgattt aatagcagca agatctgcta cgactaatag
     3061 gtcacctgca tgaacccgtt gcccttctga gactttgact gaaaaaggca caccatttaa
     3121 ggcgacggta tcaagtccca cgtgcaccag cacttcaaga ccattatctg tcagtaaacc
     3181 aaaggcatgc ttggtcggaa agacactggt cacaaggcca gctacgggtg catagatatt
     3241 accatgggtt ggttcaaccg caaacccatc tcccatcatc ttagccgcaa acacctggtc
     3301 tttcacacct gtgattggga gaaccatccc gtctgccact gatagcacat cttctgtcac
     3361 gtgtttaaaa tcttttgcgg gagtcggttt gctagtgagc tgtgacatgt tgacttctgg
     3421 aataagagcc cctgagtcca gcaaatcttg aatatctgat ttcaaaatat ctgctttagg
     3481 gccatagacc gcttgaacgc cgttgccttt ttggatcaag cccatagcgc cagctttttt
     3541 ccaatcgtcc tcagcgccaa ccttagcggg gtctttgacc gtcactcgta agcgcgtcat
     3601 acaagcatca acatcatcaa tattgcctgc cccaccaagg agattgatga tttgtacgac
     3661 ttgagagttg ctatctgcta cttgggtcgg tgttgcttga gtgttatggt cacccagtat
     3721 atcggcatcg tagttgccta aacgaccagc tgttgcgaga tgcattttct tgatcatcat
     3781 atctgcaata aagtacatga taactgcaaa gaggatagaa acccaaacaa agttaatcac
     3841 atccatgcct aggcctgctt taagggccat aggggtacgg gtcaataatt caatgttccc
     3901 aaatgagtga acacggagat tcacaaggtc agccatggca aatgaagctc cttgaacaag
     3961 ggcatagacg atgtaaagtg gcattgctgc aaacataaat aggtactcaa gtggctcagt
     4021 tacccctgtc aaaaagaccg ctgccgctgc tgagatgaac atcattttgt atgtgtgttt
     4081 tttatcggca tccacattac ggtacatggc taaggcaacc cccatcaatg ttccggtagc
     4141 tccaatcatt tgtcctacct taaagcgagc tggagtcaca ctatccatta agtgactata
     4201 ggctgaagcg tctgatcctt taaggtgaac aaggtcagtt acccaagcaa gccacaaagg
     4261 gtcttgacca aatacttttg tccccgctgc tgcacctgtc atgacctcat aagttccacc
     4321 aagagctgta tagttcattg ggatcgtcaa catgtggtga agaccaaatg gcaacaagag
     4381 acgttccaag gtaccatata ggaatggggc taggattgga gctgaatctt gtgaagaggc
     4441 aatccacatc ccaaaactgt taatcccaga ctggatcacc ggccaaacaa caaccaaaat
     4501 aagggctaca aaaatagaac gtaaaataac gacaaacggt acaaaacgct tcccattaaa
     4561 gaaggtcaaa acttcaggaa gtttacggta attatagtat ttgttatagg ccgttgctcc
     4621 tacgaaccct gcaataatcc caacaaaaac ccctgtgttt aaagctggag actccaagac
     4681 actggtgaaa taatctttaa cgatcatttg agtgccaagg aggcttgtga tttttgcttc
     4741 tggatcagct agcatagcac tagacacgcc ataaaaagcg cctgttatcc gattgatcaa
     4801 gacaaaagca agccctgagg caaaggcccc tccagctctt tctttagccc aactaccacc
     4861 aatggccaag gcaaatagca aatgaaggtt aacaataaca gcccagccaa tctgagcgat
     4921 aatgttgcct agagatgcca aaaatgctga gtcatggtta atcattggaa tagagtttcc
     4981 gatactgatc attaaaccag ccgctggcat gacagcaata acgaccataa gacatttccc
     5041 aaatttttgc caaaattcga aacgaaacag ttgcttgaat gatgttttca ttctatctct
     5101 cctttattat ccccttttag ccatagctgc ccttaaggcg cttttgtgac acaatgatat
     5161 agactacaac tcagtgatgc aatcgtttgc attggtctga tacctttatt atactagcct
     5221 cgcaacaatt tgtaaagcgt tttcttagac agatgttagt cgtttttctt cattaaatgg
     5281 tagtgtaact tatcaaaaag atgacaagag cccatttcct tttttggtga tgagcccatc
     5341 aacgctcttc aaaaaaacaa gaagcaataa aaaaaggccg ttgacacaat cgtgaactgc
     5401 ctgttcgttt ctttagcctc ttctttctaa agtaagcaac agcttactta ttttgatgcc
     5461 tgcaattcca aagcataact aagagcactt aaggcatata acggcgctgt ctctgctcgc
     5521 ataatgcgag ggcctaagcc caccttgatg gcacctgctg cctcaaactg agtgatttca
     5581 gctggggaaa ttcccccttc tggtccaaag ataaagagga gttttacccc tgatttaact
     5641 tcctttactt cacgcgccaa ggtcgctagc tgacctgcct tagctgtttc ctcataagca
     5701 atgaagatat gatcaaagct agaaaggctt tttagaaact ctgctttctg ttcaaaaagg
     5761 tgaacttcag gcaccctatt tcgtttgctc tgctcagccg ctcctaatac aatcttagcc
     5821 aatttgtctt cttttttagc taattttttg ccatcccatt tgaccactga ccagtctgct
     5881 gggtagcccc atagcgcact ggctcccaat tcagtcactt tctgggcaat cgtgtctaac
     5941 ttatctcctt taggaaatcc agaagcaatg gtcacctcaa caggcaattc cacctgatct
     6001 ggcaaggtct ctataatctc taactcatga gccatactat tggtcacctt ggctaaatac
     6061 ttcaccccat catcaaagac caagataact tcagcctcat cagccaagcg catcacttga
     6121 aacatgtgct tgatggtatc tttatcggta attgttacct ctttttctgc tttgccttta
     6181 ataaaatact gttgcattag ccaccgatca ctcctgagat atcatctgtt tttttaaaga
     6241 caagggcgtt ccactcgcct tgaatcatgt gtgtctcaag gaagaatcct gcgctaaagg
     6301 cagcttccag aaccatatcc aatttctcag aaatgatgcc tgataggata aggtaacctt
     6361 cctttttgac caaacggtaa gcatcatcag tcaataagac taagatatct gccaaaatat
     6421 tagccacaat cacatccgct tcttggctaa ccccttttaa aaggtcgcca gcagccacat
     6481 gaatgttgtc tgttccttga ttcaaatcaa tgttttcttg agccactcga acagccacgt
     6541 catctaagtc ataagcgtag atggttttag cacctagtaa ggaactggca atcgagagga
     6601 cacctgatcc ggtccctaca tcgataactg tctcaccacc acgaaggatt tgttccaagg
     6661 caaaaaggct cattttagtt gtgggatgtg tccctgtccc aaatgccatt ccaggatcta
     6721 atttaatcac tttttcccca gcagaagcgt cataatccgt ccaactaggg acaatggtta
     6781 aatcatgcgt gatgcgagct ggttcatagt attttttcca attgtctgcc cagtcttcct
     6841 cagctaattc ttgactgtcc acagtaacct gaccaacctg taacccaaaa ctagctaact
     6901 ccgctaactg ttcattaatg gtcgcaataa catccgccaa attagtgcta cttgggtaat
     6961 aggctgtgat agctatcata tcagactgct ccacatctgg gtataactcc ccaaagcggt
     7021 ccttttgctc aatatagtct gcgctatctg cgatagcaac cccttggctg cctgtctcaa
     7081 tcaacacatg tgaaacagcc tcttgcgcat cacgatgcac atgaaccgtc acttcttgcc
     7141 acgtttccat aagtatgtcc tttctatttt gaccttttgg ttattgtcct aatagtgtta
     7201 gcaccatctc gtgtgtcacc ttagtacctt gggtaagggt aacttgctgc ttcttctagt
     7261 ccttgttgac cttcttcaaa agcttgcgcg ttccactcca agaagaaatc agccttgctg
     7321 tcatctgcta agaaatccat caaatcgctg tgcccttcaa tagcaacatc gttgagaaat
     7381 tgcgcaaagt aagctaagaa ttcacgagaa aagccctttt tggcgtcaaa aggaatagta
     7441 accaaataat ctgctgcatc aaattgagac ttggcagggt tgtaaaagag cacatagtcc
     7501 tcaaagacaa tatcatcttg ggtcacttcg ccactatcat caatggtttt tatggcctct
     7561 ttattttgag cttctaaaac aaaagtcact tccaccgcgt gatttttctt gtcccaatcc
     7621 atagcatagt catactgaaa atgcttatcc atttcttctt ctaatactga taaaaagccg
     7681 tattttgcca tcttttttta tacctaatct ttctaactgt tgctgtatag agctttgtaa
     7741 gacctcatta gtttacttac tttgacatgc tgagcgtact gttataactt ttattaactg
     7801 aattataaca tatttgacta atattgacca taagagaagg tcttaatggc aggatcacct
     7861 ctagcaaccg cctttatgat ataatttggg tatgcataga aaaacgatta ttgacttcaa
     7921 agagctcggg caacgctatc tttttgatga gccattagta gaattggtag ccaagtcctt
     7981 agatcaggtc ggtccagtca ttgaaaacgt acaacactac caacagctgg gctattacgt
     8041 ggtgggctat ctcagttatg aagcagctgc tttttttgac aatgccctac aaacccataa
     8101 tgacagacta ggcaacgagt accttgccta ttttacagtg cacaaaacct gtcaaaaaaa
     8161 agacctacct cttgattatg acagcattac tatccctaac caatgggtca gcgcaactca
     8221 aaaagaagcc tatcaaaaag ccattgaaac cattcaccgt gagatgcaac agggcaatac
     8281 gtatcaggtt aactacaccc ttcaattaac tcaggaactc aatgcagctg acagcttagc
     8341 catttacaat aaactcgtcg ttgaacaggc tgctggctac aatgcctaca tcgcccatga
     8401 tgagtttgcc gttatttcag ctagtcctga attgttcttt aaacaggaag ggaatcaact
     8461 caccaccaga cctatgaaag gcaccaccaa acgaggggtt aacacctggc tggatcaaca
     8521 agaacatgac tggctccaag ctgacgggaa aaatcgctct gaaaacatga tgattgtgga
     8581 tttgctccgc aatgacatgg ggaaaatttg ccaaataggc agtgtttgtg ttgataggct
     8641 ctgtgaggtc gagcgatact ctaccgtctg gcaaatgacc tcaaccattg ttggtgacct
     8701 caaagcggac tgtgacctga tagacattct caaagctctc tttccttgtg gctctatcac
     8761 aggggctcct aaagtctcca ccatggcaat tatcacatct ctggagccaa aaccaagggg
     8821 gatttattgt ggaagtattg gcatttgcct acctgacggt cgccgtttct tcaatgttcc
     8881 cattagaacc atccagctca gtcataatca agctacttat ggtgtcggag gcggtatcac
     8941 ttggcaaagc aagtgggaag acgaatacga agaagtccag caaaaaacag tttttctcta
     9001 tcggcacaaa caaatctttg acctcaaaac aaccgccaag gtggaacacc aaaaaatagc
     9061 cttccttgag caacacctca atcgccttaa agaagcagcc acttattttg cttaccctta
     9121 cgatgagaaa gctttgcaga aacaactgtc aacctatttg gaaaataagg ataatgctgc
     9181 ctaccgtttg atgattcgtt tatccaaaga tggaaagatt agcctctctg atcaaccctt
     9241 ggaacctttg tcagccgatt ttctgacagc tcaactctct ttacagaaga aggacgtgac
     9301 agcttcgcct tttacctact ttaaaaccag ctaccggcct catattgagc aaaaatctta
     9361 tgaacaggtg ttctataacc aagcggggca attattggaa acttccatcg gcaatctttt
     9421 tatccagctg ggtcagaccc tctacacacc accagtggct gtggggattt taccaggact
     9481 ttttcgccaa gagctgctag ctactggtca agctcaggaa aaagaggtga cactagctga
     9541 tttaaaagaa gctagcgcta tttttggagg aaatgctgtc cgtggcctct atcctctcaa
     9601 ccttgagctt actcaccttg atgccctctt agctaagagt caagcctaaa ctgccactca
     9661 tcttttgtta ttaaggaacc tatgatactc ttaattgata attacgattc atttacctac
     9721 aacctcgccc aatatttaag tgaatttgac gagacgattg tcttgtataa ccaagaccca
     9781 aacttatatg acatggccaa aaaagctaac gctctagtct tttcacctgg tcctggttgg
     9841 cccaaggaag ccaaccaaat gccaaaactc attcaagact tttaccaaac aaaacctatc
     9901 ttaggagtgt gtctgggaca ccaagctatc gctgaaactc taggaggaac cttacgcttg
     9961 gcaaaacgcg tcatgcatgg gagacaaagc accattgaaa cgcaaggccc tgctagtctt
    10021 tttcgctccc tgccacaaga gatcaccgtc atgcgttacc attctatcgt tgtggatcag
    10081 ttaccaaaag gttttagcgt aactgctaga gactgtgacg atcaagaaat catggccttt
    10141 gaacaccaca ccctgccact ttttgggcta caatttcacc cagaaagcat cggaactcct
    10201 gatggcatga ccatgatcgc caacttcatc gcagccattc cccgttaatc tcatccccca
    10261 aaagaaagga ggacagttac atgcctgatc acttagcgct cagaatgcgc cccaaaacca
    10321 tttcagaggt catcggacaa aaacaccttg ttggcgaagg caaaatcatc cgtcgcatgg
    10381 tggaagcaaa tagactatcg tccatgatcc tctatggccc tccaggaatc ggtaaaactt
    10441 ccattgcttc tgctattgct ggtactacta gatatgcctt tcgtactttt aatgccactg
    10501 ttgatagtaa aaaacgtctc caagaaattg cggaagaagc taaattttct ggtggcttgg
    10561 tattattact agacgagatt catcgccttg acaagacaaa acaagatttt cttttacctt
    10621 tacttgagaa tggcaccatt atcatgattg gcgcaaccac tgagaacccc ttcttttcag
    10681 tgacacccgc tatcagaagc agggtccaga tttttgaatt ggaaccactt tctaacgagg
    10741 atattaaaac agccattcag ctagcaattt ccgacaaaga acggggattt ccttttttag
    10801 tgacaattga tgatgaggcg ctagatttca ttgttaccgc cacaaatgga gacctacgat
    10861 ctgcttataa ttccttggat ctagcagtca tgtctacttc tcctaatgaa gatagtagcc
    10921 gtcatattag tctagagaca atggaaaata gtctgcagcg tagttatatt accatggata
    10981 aaaacgggga cgggcattat gatgttttat ctgccttaca aaaatccatt agaggttctg
    11041 atgtcaatgc tagcctgcac tacgccgctc gtttagtaga agctggagac ttgcctagtt
    11101 tagcaagacg attgaccatt attgcttacg aagatattgg cttagccaac cctgacgccc
    11161 aagttcatac cgtcaccgca ctagatgccg cccaacgcat tggttttcca gaagctcgta
    11221 ttcttatcgc taacgtggtc attgatttgg ctctatctcc taaatcaaat tcagcttatt
    11281 tagctatgga tgcagcttta gctgatttga aaacatcagg taacctccct attccaaggc
    11341 atcttagaga cgggcattat gctggcagca aggacctagg caatgccaaa gattacctct
    11401 acccacacgc ctaccctgaa aaatgggtca aacaacaata cctaccagat aaattagtag
    11461 gacatcatta ttttgaagcc aatgaaaccg gtaaatacga acgcgcactt ggttcaaata
    11521 aagagcgtat tgataagctc tcagactgac tgctagtttt aaaaatcgca accgatttca
    11581 aaaatcgata attttgcttg aaaaaatttt aaaaagtggt attataaaag tacagaaaca
    11641 ttgctgtggc atacgaatcc acatctaagt gttgaccgac tatttctttg tattttcggg
    11701 aaacaaagac tctttctagt ctgcaagccg tttcacgcgg acgcaacttc caagaaagag
    11761 cgcgttaccc acctgcttat actgcgcggg ctcaatacaa aaacgtggat caccggtgcc
    11821 aatacagcat tcttgcctcc ttagctcagt tggtagagca gtagactctt aatctatggg
    11881 tcacaggttc gagccctgta gggggtatac aactatacat gaaaaaacct ttgtttaaag
    11941 gctttttttg cttgtcagaa gaggatttgt tccatcattt atccccgcag taattttttt
    12001 atggtatgat accaattaaa gcgcttaaga ttaaggtgga gactgttatg aaaataattg
    12061 agtacaaagg aaattacaga gataacctca tttttatggt cttggaagca aaaaatgcct
    12121 taaggagaat tcccagcatt aacccggatc ttttgacaat tgaaacaagt tatttggaaa
    12181 aaggggattg tttttggcta gcggtcaatg aagaaagtcg tgttattggc tcaatcgctt
    12241 attcttctat tcccaaaaca aatgaggtct tcttgcaccg tctcttcatc aaaccaagct
    12301 taaaacatca aggcattggc agtcaacttc tgacgtttgc agaagattat ctaaggaaaa
    12361 tcggcaagag tcatgtccgt atccacttag gtcaacctaa agaacaatgg tttgagtctt
    12421 atcggtttta tcctaaacat ggttatcaat tttatgatga cacgcatctc atcaagaaac
    12481 taatctaaac acatttccta ccctttatag tcatttcata cgtcattttt aggaggtatc
    12541 cattgaaaat tgccatcatt ggacatagtg gctctggcaa gtcaacctta gcacgatttt
    12601 taggtcaaca ttaccattgt gaggtggtcc atttagacca actacacttc tcatcgaact
    12661 ggcaagaacg gtcagatcat gacatgatag ctgatttatc aacatgcctg ctcaatcaag
    12721 attggattat tgaggggaat tatgccaatt gtctctatga agagcgcatg agtgaggcta
    12781 atcacattat ctatgttaat ttcagtcggt ttcattgtct ctatcgagcg tttaagcgtt
    12841 atttaaacta taggggaaaa acacgtcctg atatggctga caactgtcag gaaaaatttg
    12901 acgttgcgtt tgtgaaatgg attctactag atggacgttc aagaaaccaa ttaaaaaaat
    12961 accaatccgt tatccaaaaa taccctcata aaaccattgt cttaacaaat caaaagcagc
    13021 tcaatcacta tatgaataca atcaactaat tagagtgaaa aactaaaata gccacgggta
    13081 acccgggcta ttcagactga aagcctttgt ttaaaggctt ttttgcttgt ttcataacta
    13141 atcggcttaa ttaaaagcgt caatccactt ttagtatcat gtctcaattt tcatttgata
    13201 gtcagcaagt atatctacaa aattgttaat gattatttct tttgaaagtg ttatattaaa
    13261 acataataaa ataataactc ctgaaaagag gctatttatg aaaaaaacaa aacttatttt
    13321 ttctttgact tcaatattca ttgcaataat ttctcgtcct gtgtttggat tagaagtaga
    13381 taataattcc cttctaagga atatctatag tacgattatg tatgaatatt cagatacagt
    13441 aattgatttt aaaaccagtc ataacttagt gactaagaaa cttgatgtta gagatgctag
    13501 agattttttt attaactccg aaatggacga atatgcagcc aatgatttta aagctggaga
    13561 taaaatagct gtgttctccg tcccatttga ttggaactac ttgtcagaag gaaaagtcat
    13621 agcatatacg tacggcggaa taacacccta ccaaaaaact tcaataccta aaaatatccc
    13681 tgttaattta tggattaatg gaaagcagat ctctgttcct tacaacgaaa tatcaactaa
    13741 caaaacaaca gttacagctc aagaaattga tctaaaggtt agaaaatttt taatagcaca
    13801 acatcaatta tattcttctg gttctagcta caaaagtggt aaattagttt ttcatacaaa
    13861 tgataattca gataaa
//
LOCUS       DAUGFP010000040        13379 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.040, whole genome shotgun sequence.
ACCESSION   DAUGFP010000040 DAUGFP010000000
VERSION     DAUGFP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.040"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..1807)
                     /locus_tag="VH663_001270"
     CDS             complement(<1..1807)
                     /locus_tag="VH663_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEQ8174468.1"
                     /translation="MIKDTFLKTNWLNISHHITLLVFGFYFSFYSLAKELVSSTAQPV
                     NYYTHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLCYQGNTFS
                     VALILAVVPISDIHLLKLISSRFSELVRAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLKSNKPSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVALLAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPN
                     IKAIKNSTTAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHT
                     ISEFYQGKNRIAMHPASANNFNRKTVYSNLGFSKFL"
     gene            complement(1804..3549)
                     /locus_tag="VH663_001271"
     CDS             complement(1804..3549)
                     /locus_tag="VH663_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEQ8174469.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMGPYFENFEEKEAVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKTESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSAFETNDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLLVYI
                     AWGNSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPA
                     KAMKYIFLRLMEKLK"
     gene            complement(3546..4553)
                     /locus_tag="VH663_001272"
     CDS             complement(3546..4553)
                     /locus_tag="VH663_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ8174470.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTIYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEVSELLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            complement(4578..5783)
                     /locus_tag="VH663_001273"
     CDS             complement(4578..5783)
                     /locus_tag="VH663_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174471.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            complement(5783..6586)
                     /locus_tag="VH663_001274"
     CDS             complement(5783..6586)
                     /locus_tag="VH663_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174472.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGVDVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYIVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            complement(6589..7521)
                     /locus_tag="VH663_001275"
     CDS             complement(6589..7521)
                     /locus_tag="VH663_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ8174473.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLNPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            complement(7511..8665)
                     /locus_tag="VH663_001276"
     CDS             complement(7511..8665)
                     /locus_tag="VH663_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEQ8174474.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEGNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWTK
                     QKGQLAELINQVDAGFDSDRLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            complement(8784..9638)
                     /gene="rfbD"
                     /locus_tag="VH663_001277"
     CDS             complement(8784..9638)
                     /gene="rfbD"
                     /locus_tag="VH663_001277"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEQ8174475.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            complement(9776..10114)
                     /locus_tag="VH663_001278"
     CDS             complement(9776..10114)
                     /locus_tag="VH663_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEQ8174476.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            complement(10350..11459)
                     /gene="rpoD"
                     /locus_tag="VH663_001279"
     CDS             complement(10350..11459)
                     /gene="rpoD"
                     /locus_tag="VH663_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEQ8174477.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            complement(11468..13282)
                     /gene="dnaG"
                     /locus_tag="VH663_001280"
     CDS             complement(11468..13282)
                     /gene="dnaG"
                     /locus_tag="VH663_001280"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ8174478.1"
                     /translation="MGFLWGGDDLAIDKEMIFQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNISSSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     ELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
BASE COUNT         4190 a         2603 c         2175 g         4411 t
ORIGIN      
        1 ctaagaattt ggaaaaacct aaattactat aaactgtttt tctattaaaa ttgttagcac
       61 tagcaggatg catcgcaata cgattttttc cttggtaaaa ctcactaatc gtatgaggtt
      121 tggccatttt aggaaagact tctgaataaa gaacagatac tgaagaagaa aaattataaa
      181 aaggtaagct tgttaacgtt tgaaactcca tgttagcagt tccacccccg taggagtctg
      241 actgcatgag tcccgcagtt gtgctatttt tgattgcctt gatattaggt aaaacatcgt
      301 ggctaacagt aacatttgat actctatcag gatctgacaa actttcactt aaaagataaa
      361 taaccgtttg gtcagcaata ttattttttc tgtctttatt aatatcttct gctaattttt
      421 ggtacttctg agcgatactc gctattgttt cttggctata atttgtcggt ttttccatta
      481 ttttcttggt cacctgtctt gtccaaacat atgctaaaga tttgtaccta gcatttgttg
      541 aaaaacctaa ccagtttata tcattatcat tatttaaagc cgaaataatc ggaagaccag
      601 ataatatttt accttctttt tcataagaaa atggtattaa catactacaa gatacaataa
      661 ataaacctaa tacagcactt gcccgtttga cagggctcat aaataatttc cctgctaaaa
      721 ctcggccatg tagataccaa gccaccacaa tacaaatagc aagtcctaca accatttcta
      781 caatcaccga ttttttaaca aagcttgtca ctaaacctaa ttcttgtaac caaacaaaat
      841 cagttactaa taacggctcg cttctcattg attcttttaa atcattaaca acagaaataa
      901 ttgttcccag aataagcaaa aagaaggtag ttggccaata tctattcgtt atcacgtatg
      961 ctaaaagagc aactagtgta atagctacta tctgaaaaag agtagctcca gggaaaacat
     1021 aatatcctag caatgcttca tcacctttta ctccatactg gaacgtatag ttaaaaacta
     1081 atgctaaaaa caaactagta gtagctgcta ctgaaaatga aggtttattt gacttaaggt
     1141 gttttagagc atttaaaacc acgtatatga ttgttgcaaa tatgacaagt aaaactatca
     1201 acagtccact tatttttaac agtgcaattt ggctattacc tgctcttact aattcagaaa
     1261 atcgagaact tattagtttt aacagatgta tgtcactaat tggcacaact gctaaaatca
     1321 gagctacaga aaaagtatta ccttgatagc ataatagccc aaatctatct ttttctattg
     1381 caaaatatgc taccaatatt tttattaaag tggccatcac taaaataatg agtaaagaag
     1441 gtagagcttg aaattgataa aattgatttt tagttaatga ccagatatca aaccgtttat
     1501 cattcaggtg ctgtgttatt tgtacccaat agctgacaat tagataagat atcacaataa
     1561 aactagtttt caaaaacagc tgtcgactaa cttggcgact caaataataa gataatccaa
     1621 tcagtgatat aatatatccc acaaaagaaa catttagtaa atgagtataa tagtttaccg
     1681 gttgtgccgt ggagcttact agttctttcg ccaaactata aaaactgaaa taaaaaccga
     1741 aaacaagaag ggtaatatgg tggctaatat ttaaccaatt ggtttttaaa aatgtgtctt
     1801 taatcattta agtttctcca ttaaacgcaa gaatatatac ttcattgctt tagcgggacc
     1861 aacgataata tattttaatg ctcctttgat accgcccatt tgattaaagt taacgtaggt
     1921 atgagctcct tcatcttcac gtaaatttaa cagtttattg tcaataaaag gtgttaactc
     1981 atagggattt ttaacaatcc taaaatcata agaattcccc cacgctatat agacaagtaa
     2041 ccgctcaatc gcatgtaata tagagttttg tggtaaaggc tcacttggaa tatcattttg
     2101 agttaattct aaatcaaaaa gtgattttag agcatcgtat ttaaaccata caaatgttcc
     2161 ataactcata acaaatgtat ccatagcttg aaaatcaatt tgttttttaa catccatttt
     2221 tctccacaaa gacatcattt cttgagcgat taaatgctca ttccaagcat tgacaatctt
     2281 attaaatcta aaaaaacttg gaatatctgc gattataata ccaatatcat ttgtctcaaa
     2341 agcgctaaga atactatctg ctggtttcac gagcatatca atcaattctt ttcgccaaga
     2401 ttctcctgcc caaaaatcag cttcttttga ttttttcgta tggaaatgac caatataatc
     2461 atattgagaa agtttatctt ttagtaataa cattggataa atatctcttc ctctatttcc
     2521 cgtaacgcgt atatcagcag ttttaccttt tctttgaagt atttctttga tctcttttct
     2581 tttgatatca ctatctgttg taataaaaag atcataatgg aagttccagt cttcgaaagc
     2641 tgttaaaaat tcatctagca aatcaacgta aaaaacatgt aaatgaacag ctaccttttg
     2701 acaagtttgt tttgctaacc gttggcagtt taggtatttt tgagataaaa ggtacttagc
     2761 atctggcaaa gagatttgcg acatatgtga aacaataaga tctataggat aattggttgt
     2821 ttctcgtatc agattcagta agtaaggagc gatatgctgg ttagcatcaa tagcttttac
     2881 ttttataaaa ggcacatggt gttttaaaat tgctgttgga ttataatatg aaaaatccgg
     2941 atgtggcatg aatgacgact cagtttttct agtatcaaag accgtctgat aactaaagcc
     3001 ttcatttaag agaatggatg ttaattgcgt ttcgtaatgc tgaatcactt cttgaacgtt
     3061 ttcatattct attatcgatt gccaaaattg ttgaaaaact ttgttttgaa tgacttgatt
     3121 tttaaacgtc ataaaataac tttggacatg ttctttaaaa gcttttgtac ctctattatt
     3181 agtaattccc caaaagtcaa ctgcctcttt ttcttcaaaa ttttcaaaat aaggacccat
     3241 ctcccaaata ggaccaaaac aagtatcgtt cataatcgtc aatgagtcaa actcttccaa
     3301 cttgtcaaat cccatagtaa tcaagccatc gtgccatgca gaaaaatcaa aacctttatt
     3361 tttgcgttgc aaaaactcat caataagaca gtggtttttg agacgtttta aatcttcatg
     3421 actcacttta ctatttgaga taaaaacaat cttagaaaac agtgatctca tttgttccaa
     3481 ttgatagtaa acatgtgcac ttattttatt gtatttgttg aagtgaacat ataataaaat
     3541 acgattcatt tttttctcct aataaaagca atgagcttag acgtgtattt ccattgacgt
     3601 gaattaataa tggcatggta ttgttgtctt aattcagata gttgattttt ttgttcttct
     3661 atgacatttt gcaactccac ttcacgtgtt tcttgctgct ttgttttatc tacgatagtt
     3721 tgttgtaatt cttgaataaa gcaactcaac tcatgaattc gtatttcttg cttagcatct
     3781 tgttgtaata atagttcaga cacttctttt gtatctacct gattatactt ttttaagtaa
     3841 tttaactgag tatcaagtag tttttgaata cgatgaatca tttttggtgt atcagtaggt
     3901 cttgaatcag aatcggttgt catgcgataa atcgtcgttg agtctttaat ataggtcagt
     3961 tgagtcagtt gaaataactc tagttgaata tcaaaagtat cgtctggtgt atctacattg
     4021 attttttggt ttacttgtct cattaaatca gcatcaacta accatgtcga tgccatcgtc
     4081 atccctttta aggctaaaac tttttcgtaa gtatctgtca aaggtgtatg accagatgaa
     4141 aaagcattag catataataa gtcgccttga ctatttacaa agtcaaaatc agtattacac
     4201 catcgtgacc gtttggaagc ttctaagaca tcaacttgtt tttggagttt aaaactatcc
     4261 gtccagtaat catcaccatc acatctagca atatatttgc cttttgcata aaggcatgct
     4321 tttatccaag tctttgttat tccaatatta gtttcgttaa agagtaattt aatttttcct
     4381 gaagactttt tttgataact ctttaaaatc tctcgtgagt catctgtaga agcatcatca
     4441 ataacaataa tttcaacttc aaaatctgtc acttggctta aaaaactgtc aagtgcatca
     4501 gatatccaag gagctttgtt ataattggtg caaataattg aaactttata cataaattcc
     4561 cttctagcta ttactcatta atgaaaaacc cacttcccat tacgttgata caagccactt
     4621 gctgaatcta gagctgacag atcatcttga tcaatatcgt ttctttgaat cactatctgg
     4681 ggtgattgtt ctgctgttga aaagagtagc atctgaccgt tcttatctcg gacagttact
     4741 tctaatttta gttttatatc attaagttga gatagttgac attggtagga aatgcatttt
     4801 ctacctatac cttgtgttgg ataatctaaa gaattgtcgt tgtaaaccca aatgtttcta
     4861 tcaatatccg ttaaagaaaa ggcaatgtag gtaaaatcat cttttaaaac ttcataagaa
     4921 acttcaaatg tgattgtgtc atttggtgtt attcttggat ttgagattaa tgacactttc
     4981 aaatctttgg caatatcaga tacagatatt ttttcagcat tcattgcatc ctcagctgtc
     5041 tctgtattat ctacactata ctgattagca acatcaaatg gttctccata tgctttaaca
     5101 agaccatctt caataagaac tgcacggtta caatacttct taacagctcc catatcatgt
     5161 gtcactaaaa tagtcgtctt accactgtct tttctttcca taaaataatc attacatttt
     5221 cgctgaaaag cttcatctcc tacagcaagc acctcatcta aaataagaac gtcaccttgg
     5281 gctttaatag ccactgaaaa tgctaaacgt acctgcatgc cagaagaata attttttaat
     5341 ttttgattca taaaatgatg caactctgca aaatcaacaa tatcattgta catatcatca
     5401 acttcgtctt tggtaaatcc cagcatggcg ccgttcatat agacgttttc tcgtcctgtt
     5461 aattctggat tgaatcccac tccaagctct ataaaagaaa ccatcttccc atctactgta
     5521 acctgtcctt tttctggaac atagatctgg gaaataattt taaggagcgt tgattttccc
     5581 gagccgttac gcccaacaat tccaaaaaaa tcgcctttat aaacatcaaa gttaatatct
     5641 tttagcacat gttgttcagt aaatccttta atccctctta ggcgattaac cagagtcgtg
     5701 cgaaaacttt tagttgcttc tgtaggcagt ttaaatgttt tactaacatg ctgtactttt
     5761 actgcaattt ctctattaat cattaaataa tctccgcaaa tctatcggca tttttcttaa
     5821 agacaaagat gccaataaat aatataacaa atggtactaa atatggaata acaatgtaaa
     5881 accaattagt tgacatctgc caaatcgtta cgttggctct gtcaatcaat aaataacgaa
     5941 aatcttgaat catttgtgct actggattta gcatcaacaa ctttgccgcc aaagggtggc
     6001 tatctaaaac aaaagtgatc ggataaatga ttggagtggc atacatacct gcttgtaata
     6061 gtacttccca gacttgagct aagtcacgat aataaacaaa gacattcgac aataacaatg
     6121 caattccaag cactaaaaca actaattcta taaaaagaaa aagagagaga taagcatacc
     6181 ctgatatagt cacaccattt atcaacgcaa aaattaaaac aacaaccaaa ttaataagaa
     6241 aattaattaa agctcctaac actgccgaaa aaacaatgat gtgcttagaa aagttcaatt
     6301 ttcgcaacaa gtctccccga gatacaatag ataccattcc catgctagtt gcttctgaaa
     6361 aaaaagacca gatgacattt gccaataaaa gcgctactgg aaaatgaggt acatctacac
     6421 ccaggcgaag aaaacgaata aataccaagt acataatcgt aaacatcata agcggtttta
     6481 agatagacca tagatagcca attgcgctcc cttgatagcg taatttaaaa tccgttttta
     6541 ccatttctct cagtaaaata cggtttttct ttgttaaaaa attcattctt actttctcct
     6601 gtaaccaaat aaggtaacaa ctaaactacg aaaaataaag gtatgaaaga tgcgattttt
     6661 tctaaaaccg taccttttca aggtagctaa gcgtttggta aaaggcatat ccaataaaga
     6721 aacataggcc gttaccaatt catggtcatt tggttttaat ggtaaatcta atagtaattg
     6781 agcctgtttt tggcttgaag taatcaacca ccaatatttg ttgactaaat gatgggggtt
     6841 taaccaattt ttcatgcgtt tagaccaagt tctagctccc aacacgttag catcgtgctg
     6901 acggtataat tctgttggaa tatcaaggta aactaatttc ccagtagcag aagctagtaa
     6961 agctaaatac cagtcatgca tcaataaacc gtcacaagtg gtccattcct cagccagagc
     7021 atgagtaatc atcattgtgc cgcctgttac ggtattttcg gttaattctt gaagtaggct
     7081 tgtgtttgca tgaccagatt gggtcttaat catactgtca tggcaaatag ctaaatgttg
     7141 ggttaccact ttgaggtctg tataaaccaa taaaggagct gtcatttcat gtttttgagc
     7201 ctccaataag gtcacttcca gtttattgtc taaccatata tcatcttggt cactgaaaaa
     7261 atagacatct gctttttggt gctttaagag ggtgtaaaaa ttcttaataa ctcccagatt
     7321 ttctgtctgc ccctcattga tccactgaat tcgtttgtct tccttgacaa atgtgcgaat
     7381 aatatcctgg gtgccatcag ttgaaccatc atctctaatc aataaggtcc agtcgttaac
     7441 tgtttgcctt tggatacttt gaatttgttc tgctaagaag cgttctccat tgtaggtgga
     7501 tagtaaaata ttaatgttca tttaaaaata aagcctcgta ctcccctaca attttttccc
     7561 aagtgtaatg ttcttgaata atagctttgg cttctttgcc cagacgatca ctatcaaaac
     7621 ctgcatcaac ttggttaatc agctctgcta attgcccttt ttgcttggtc caataaagcg
     7681 cagcagactt agccaccgac tggttaaaat caactcctaa aaccaaatta agattggtat
     7741 gggctagtgc ttctagaagg cctggattag tccctcctac ctcatgacca tggatatagg
     7801 cataagcctg ttctcgaatg taggctaata gttctttgtc ataaagggta ccaacaaatt
     7861 taatgcgtgg gtctttatca cactctgtct cagcaagtaa ttgcttaaag taagcattgc
     7921 cctcatgatt acaaataatc actaaatcac gcttagttga ggaagccata aattctttaa
     7981 tagcagtttc gtaattattt tcaggaacaa agcgtcctaa aatgagatag taatcctttt
     8041 ctctaatatc aaaggtttga aaataagctc tgaccttgct atctgctgtt gtcaaacgtg
     8101 aaggttgtgt ctgcgttcca taagcaataa aacatgtttt agaccaagga taaacctgtt
     8161 taaggtagcg gtcaatgcca atattatctg aaatgaccaa gtcagcctgt ctagtcattt
     8221 gcttttcgga aaacttcagg taggcctgca ctggttttga ccatttagac cgtttccact
     8281 ctaacccgtc tggattgata aaaaagcgtc cacctcgatt gtgaatctgc ttcacaaatg
     8341 gcgcaataaa agcgcctaca gtattgccca acacataaaa aatagggttc tgaatctggt
     8401 gttggtcgct ataagacagg gcataggtaa ttgccatcat atcataagca ataactctag
     8461 caggaccaag ttttggggga ttaaggtaaa agcaatctgc ccccttataa tcaaaatgaa
     8521 ccttatgctt cgtatcgctt aaacaagcaa cgtgataacg aatgttttta gatgattgat
     8581 gactaatcaa ctcttcgaca aaggtttcaa agccaccgta tttcgctggt aaccctctgc
     8641 taccaatgat aaaaacatcc tgcattgcat actaccccat tctaaaatat aatcagtgat
     8701 attataccac aattagaagg ctaatgcgac cttaaaccca tgaaaaagag ttggccttag
     8761 aaagccaact ctttttagaa tacttacttt ttcagtcctt gttggtaaaa tgcctttaag
     8821 gcttcttgcc atgttggaat aacaaaccct gttgcttttg ccttatcaag attcatggtt
     8881 gaatttaaag gacgtttggc ttttgctggg aaagctgatg aatcaactgg caccacttca
     8941 acagccttgt cttttaagat ttctttggca aaatcatacc aagtggtgtc ttccttagca
     9001 tcatttgaca agtgatagta accaaaggct ttttgattct ctgtcaaata acacatgaat
     9061 tctgccaagg ttcttgtcca cgttggccga ccgtgttggt cattgacaac agtcaaacgg
     9121 gagtgatttt ccgctaactg ttccatggta aagacaaagt ttttaccata atttccaaat
     9181 acccaagcag tacggataat ataaaaatgc tctgcgtatc gttcaacagc tagttctcct
     9241 aaacgttttg tacggccata ttctgtctta gggtctggac gatctgtttc tacccattct
     9301 tgaccgactg gtttattgcc atcaaatacg tagtctgttg aaatgtaaac aagggtagcc
     9361 ccatattttc cacaagcctt ggcaatattt tctgagcctg ttacattaat agcctcattc
     9421 aaggcttttc cttcgtcttc tgctgcatca actgccgtat aagcagcaca atgatagacc
     9481 aatgttggct tgacctgcgc aaaaacagct tcgactttgt cttcatttgt gatgtccatt
     9541 tctgcgacat caacggctac ataatcaaca cctcgttcat ctaataagta tcgaagttct
     9601 gtccctaatt gaccattgct tcctgtaatt aaaatcatca gttctccttt ttgccatagg
     9661 tagaacactt ttctcagtat cttagtgttc tctaacatta accaaaggct ttcacctgta
     9721 tttcctttta ttctacatca aaaaagggaa tatttcatcc ctttactatc aatgattagc
     9781 gaatacccag cgcaatccta gcatagcggc tcattttatc aaccgtccaa gcagggtacc
     9841 agactaattt gacctcagtc ttagtgactt ctggcacatc ttgcagggca tcatggatgt
     9901 aatctgtcaa caagtctgcc aaaggacaac ccattgttgt gagtgtcata tcaatctctg
     9961 tataaccgtt atcattaaac cgaatttcat aaatcaagcc tagattaaca acatcaatac
    10021 ctagctcggg atcaataaca gtttctaaag cttcaagaat tctattcttg atagctatca
    10081 cctgatcttg tgtatatttt ggtgtatcag acatggcttt cctttcattt tttgaatatg
    10141 tcgtagataa gtaaaaaaac cactaaagct ttgcctattt agaggaattg ccctctgggc
    10201 ttgtttaaga tatcaagtct acccttaaaa atacccacca cattcggtcc gttttctaat
    10261 ttgtaggtgt accgctgata agtgccactt gggattttcg ctcctttctt atttcaaagg
    10321 aacgacctaa aatcgtccac tggacgattt tagtcctcta taaagtctct taattgttta
    10381 ctacgactag gatggcgaag ttttctaagg gctttagctt caatctgtcg aatgcgctca
    10441 cgagtaacat taaagacttt acctacgtcc tctaacgtac gcattttacc atcgtcaagg
    10501 ccaaagcgta ggcgaagaac gttctcttca cggtcagtaa gcgtatctaa aacttcatcc
    10561 aattgctcac gaagaacaac acgcgtcgta taatctactg gattttcaat aacttcatct
    10621 tcgataaaat cacctaagtg actatcatct tcttccccaa ttggtgtttc taaagaaaca
    10681 ggttcttgag caatttttag aatttcacga accttgtctg gagtcatttc catacgttct
    10741 gcgatttgtt ctggcgttgg atcttgacca agttcttgaa gaaggttacg ttgttcacgg
    10801 actaatttat taatcgtttc aaccatatga acagggatac gaatcgtacg tgcttgatct
    10861 gcaattgcgc gtgttatagc ttgacgaatc caccatgttg cataagttga gaatttgaat
    10921 ccttttgaat aatcaaattt atcaacagct ttcatgagtc ccatgttacc ttcttgaatt
    10981 aaatcaagaa actgcattcc acgtccaaca taacgttttg caattgagac aacaagacga
    11041 aggtttgctt ccgctaaacg ttgtttagcc attaaatctc ccttggcaac cgcaacagcc
    11101 aattcttttt cctcttcact tgttaaaagc ggtaccacac caatttcttt gaggtacatg
    11161 cggacagggt cgtttacttt agctgaatta ctaccaatta attcttcatc agttaattct
    11221 tctggttttg gttcttcaac aatgtatttt gatgatgggt taccttcttt atccgtaatc
    11281 gagataccac catctgttaa ccgctcaagt aagtcatcaa tctgatcagc atccaaagca
    11341 aatggaatga caagtttttc ggtcacgtca tcatcaatcg ctgttccttc ttttttgtga
    11401 tggcgaataa actccgcaac ttgaacgtta aaagttgtta tttctttttg ttttgtcata
    11461 atttcctcta ttccattttt cgtttctgcg caataaaatg ttctagtact tctagagccg
    11521 cttgatgatc gcctttatta ctagattctc taactttttt cccttgtttg tgaagatcgc
    11581 gctctgctaa aagtttggca cgtttggata aaatatcatc aatctcacca agagctactt
    11641 ctttgggaag gttttcttct aaaacattat aataagcacg gttaacttcc tctgacatct
    11701 ctgacaaatc ataagatgta atgtgtcctt gttgcttcag ccgttgataa agtaattcta
    11761 aggtagaggt atcaaaataa aaatcatcac gatgtcgaaa ttcatttaat aaatagtcat
    11821 gatgtaagag ccgatgcatg agatgacttt ctgtcttagc aatagctgtc aaactttttg
    11881 gcattggtaa ggtcacaaga ttgctgacag cttgagctat ttgaccttga cgcttttgcc
    11941 tatcttgaat ccttaatgca tttactgatt gttctacttg aaaatagtca aagtttggca
    12001 acaaatcagc aatcttgtta atatacgaat gttgagctgt gatggatggt gattgagcaa
    12061 tcaatggtgc cattttctcc acataaacaa tttgtgattg caaattgtct acattagtag
    12121 gttttaggta atcaataaaa aattcaacac tactgatccg tgactgctta agcaaatctg
    12181 caaatgcttc tggggaatgc cgttgtacaa attcgtcagg atccatttta ttggggattc
    12241 tgacaatttc gacaacaaaa tctttaagca attctagtga ttttgcaata gcatgttgtc
    12301 cagcatcgtc accatcataa atcaaaacaa cttttttagt gacttgctta aggtgattga
    12361 catgttcttg agtcaatgcc gtccccattg aagcaacagc attttcatag ccggaacggt
    12421 aagcggcaat cacgtccata aacccttcca ttagaaatac ttcatgggtt ttggcaataa
    12481 caggccttgc cttgtccaga tgatacaatt cataagattt gttaaaaaga actgttcctc
    12541 ttgaattttt atacttggcc tgtctctttt ctgtatcagc tgccgtccag atacgtcctg
    12601 aaaaggcaat aatatgccct cggtcatctg ataagggaaa catgatacga tttcgaaagg
    12661 cgtcgtaaat ggtattagat tgatcggaca agttaaacaa tcctgaagca accaattgac
    12721 cttcctcgta ttttttagaa agagcttgat aaagataatc tgactcatct ggggctaaac
    12781 caatattgaa atgctcaatt aattggtcat ccaagcctct ctggtaaagg tacttcctag
    12841 cttcttgacc aatggtagtg gtcatcaaaa ctgcatggta aaatttagca gcatcctcat
    12901 gaagtgtcat caaagcgtga ttagggtgct tgtgttggct agcaagtact gcctgacttg
    12961 acgatatatt aagcgacata ccagtcttat cggcaataat ctgaacactt tctaagaagg
    13021 ggacttggcg gtattcctca ataaatttaa aaacatcccc tgattttcca cagccaaagc
    13081 agtgaaaaaa ttgtctgtct tcaacaacat taaaagaggg tgtcttttcc ttatgaaatg
    13141 ggcaaagccc gagataatgc cgccctgatc gggaaagttt gaccacttct ccaatgacat
    13201 cgacaatatt aacgctattt tttacttgga aaatcatttc tttgtcaatt gccaaatcgt
    13261 cacctcccca taaaaatccc attttagttt tacgttttat tataccaccg aaactctttt
    13321 ttgtaaattt aaaaactgaa gtgattccat tggattttgt ttactaatta tgctatgct
//
LOCUS       DAUGFP010000041        13340 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.041, whole genome shotgun sequence.
ACCESSION   DAUGFP010000041 DAUGFP010000000
VERSION     DAUGFP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13340
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.041"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            537..1064
                     /locus_tag="VH663_001281"
     CDS             537..1064
                     /locus_tag="VH663_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEQ8174479.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            1064..2182
                     /gene="yqeH"
                     /locus_tag="VH663_001282"
     CDS             1064..2182
                     /gene="yqeH"
                     /locus_tag="VH663_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEQ8174480.1"
                     /translation="MEELFCIGCGIQIQTEVKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIERINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGNKDVITTSRFPGTTLDKIEIPLDDGTFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLSRFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPGKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            2207..2515
                     /gene="yhbY"
                     /locus_tag="VH663_001283"
     CDS             2207..2515
                     /gene="yhbY"
                     /locus_tag="VH663_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEQ8174481.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            2584..3216
                     /locus_tag="VH663_001284"
     CDS             2584..3216
                     /locus_tag="VH663_001284"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEQ8174482.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDYYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            3213..3806
                     /gene="yqeK"
                     /locus_tag="VH663_001285"
     CDS             3213..3806
                     /gene="yqeK"
                     /locus_tag="VH663_001285"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEQ8174483.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            3830..4183
                     /gene="rsfS"
                     /locus_tag="VH663_001286"
     CDS             3830..4183
                     /gene="rsfS"
                     /locus_tag="VH663_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEQ8174484.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILILDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWHEAPAVALDAYLA"
     gene            4231..4974
                     /locus_tag="VH663_001287"
     CDS             4231..4974
                     /locus_tag="VH663_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ8174485.1"
                     /translation="MEHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEAPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTKTSKRWFFVAYK"
     gene            5228..6334
                     /locus_tag="VH663_001288"
     CDS             5228..6334
                     /locus_tag="VH663_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEQ8174486.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFISVQSADYFAQGAIDILCQLGIQQLAFGTENVID
                     YQKLIKVYEKKSKQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
     gene            6816..7532
                     /locus_tag="VH663_001289"
     CDS             6816..7532
                     /locus_tag="VH663_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ8174487.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            7735..8577
                     /locus_tag="VH663_001290"
     CDS             7735..8577
                     /locus_tag="VH663_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ8174488.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYKVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            8904..9749
                     /locus_tag="VH663_001291"
     CDS             8904..9749
                     /locus_tag="VH663_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ8174489.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKTKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            9999..11063
                     /locus_tag="VH663_001292"
     CDS             9999..11063
                     /locus_tag="VH663_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174490.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKIQLSANALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQKFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            11064..11756
                     /locus_tag="VH663_001293"
     CDS             11064..11756
                     /locus_tag="VH663_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174491.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSIFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(11810..13180)
                     /gene="brnQ"
                     /locus_tag="VH663_001294"
     CDS             complement(11810..13180)
                     /gene="brnQ"
                     /locus_tag="VH663_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEQ8174492.1"
                     /translation="MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIG
                     FCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGA
                     TSFSVGIAPILGDNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
                     VIASFVHPAGNYGDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEAT
                     KQFGAKTDKEVTKITLISGAIAILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPV
                     NGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWA
                     TIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIG
                     LTFIPAIFDALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLIL
                     SKTKKS"
BASE COUNT         4402 a         2090 c         2772 g         4076 t
ORIGIN      
        1 ggtaaatcta tgaaaaaagt gtaatcattt tttgatttac agttattatc atgtgttatc
       61 tggcgactgt cctgtttaca agtaagttca caagtatggg ataaaatagt ataaactata
      121 gtaaatactg aatagtcaaa gaaaaatatc ggcataggtc agaagagctg atatgtttaa
      181 tactttttgc ggatatctac tgtatttaaa tgtatttgat atccttgtca aggatatacg
      241 tctaaacaag ttcaaaacga aatcatttgt gattatctta tcaggagttg actcttgata
      301 agatttttgt atatcagtac tgtggtacgt ttagatagct tggcagtgaa tttatagagt
      361 gtcaaacaac gagtgttttg tgttacacta accattagaa gagtctattg ttgagcatat
      421 caaggtgtta tcttttcgag ttgaggtatc atatctagta tatttaacta tggttttaaa
      481 tgctatttca aaaaaaatat gataaaatag aaagtactaa ttattttgga gatgacatga
      541 gtatcgatga ttataggccg acctatatgg tagaggctat ttatgattta agagcaaatg
      601 atttgttgcg tcatggtatt acggctgttt tagtggattt agataatacc ttaattgcat
      661 ggaataatcc tgatggcaca ccagaggttc gcgcttggct tgatgaaatg actatcgcgg
      721 atattagcgt ggttgttgtt tcaaataata aacattctcg tgttgagcgt gccgtttcac
      781 gctttggtgt tgactttatt agccgggctt tgaagccatt tgcttatggt attgaaaaag
      841 ccattgctcg ttatggattt gatcgcaacg aggttatcat ggttggagat cagctaatga
      901 ctgatatcag agctagtcat agagctggaa taaaatcagt tttagtcaaa ccacttgttg
      961 cttctgatgc ttggaatact aaaattaacc gctggcgtga acgccgtgtg atggcaaaat
     1021 tagaagagaa atatggtaaa ttatcatatc aaaaaggaat ttaatggaag aattattttg
     1081 tataggctgt ggtatccaga ttcaaactga agttaaagaa aaagctggtt ttacgccagc
     1141 tgctgctttg aaaaagggta tggaaacagg agaactctat tgtcagcgct gctttagatt
     1201 gaggcattat aatgaaatta cagatgtcca tattaccgat gatgaatttt taagacttct
     1261 tcacgaagtg ggtgatagtg atgctcttgt ggttaatgtg attgatattt ttgactttaa
     1321 tggttctatt attccaggtc tatctcgctt tatttctggc aatgatgtct tactggttgg
     1381 gaataaaaaa gatattttgc caaagtctgt taaggatggt aaagttactc aatggttgac
     1441 ggaacgtgca cacgaagaag gtcttcgccc tttagacgtt atgttaacaa gcgctcaaaa
     1501 taaatatgcg atcaaagact tgattgagcg tatcaatgag ttacgtaatg gtcgagatgt
     1561 ttatgttgtg ggggtcacta atgttggtaa atcaaccttg attaatgcga ttatccaaga
     1621 gattactggt aacaaggatg tcatcacgac gtctcgtttt cctgggacaa ctcttgacaa
     1681 aattgaaatt cctttggatg atgggacgtt catttttgat acaccaggaa ttattcatag
     1741 gcaccagatg gctcattatt tatcccctaa agagctaaaa atagtgagtc ctaagaagga
     1801 aatcaagcct aagacgtatc aattaaaccc agaacaaact ctctttttag gtggtttgtc
     1861 tcgctttgac tttatcaatg gggagcgtca agggtttaca gctttttttg acaatcaact
     1921 agaattacat cgtacaaaat tagctggagc agatgctttt tatgataagc atgttggaac
     1981 tttgttaact cccccaggta agaaagagtt gacagcattt cctaaattag ttcgtcatga
     2041 atttacaatt gatcagaaaa tggatattgt tttttctggt ctaggatgga ttcgtgttaa
     2101 tggtcaaaaa gactcaaaag cgatagttgc tgcatgggct cctgaagggg tcgctgtcat
     2161 tgttcgtaaa gcgattattt aaaaataaaa aagaagttgg aaaaaaatgc ttacaagtaa
     2221 acaaagagct ttcttaaaat ctgaggctca tagcttaaaa ccgattgtac aaattggcaa
     2281 aaatggatta aatgaccaca ttaagactag tattcgtcaa gctttggatg ctagagaatt
     2341 gataaaggtc actttgttgc aaaatactga tgaagatatt catgaagtag cagagatttt
     2401 agaagaagaa attgggtgtg atacggtttt gaaaattggt cgtatcttga ttctctataa
     2461 ggtttctgct aagaaagaaa accgcaagtt gtcccctaaa gttaaagcaa tctagtcctt
     2521 ttatttgtat aggggctggt cttatgagac gaggactatt gtcgcgagaa ttttggagaa
     2581 agtatggcat tagaattgtt gacgcctttt acgaaggtgg aattagagga agaaaaaaaa
     2641 gaaagcaacc gcaaacagat tgggatttta ggagggaatt ttaatcctat tcacaatgca
     2701 caccttgtgg ttgcagatca agttcgccag caattaggat tagatcaggt gctattgatg
     2761 cctgaatgta aaccaccgca tgtggatgcc aaagagacta ttgatgagaa acatcgtctt
     2821 cgcatgttgg aattggctat tgaagatgtt gaagggctag ctattgaaac ctgtgaactt
     2881 gaacgtcagg gaatcagcta tacctatgat acgatgcttt atttgactga gcagcatcct
     2941 gatgtcgatt attattttat catcggagct gatatggttg attatttgcc aaagtggcac
     3001 cgtattgatg aactggtcaa actcgttcaa tttgttgggg ttcaacgtcc aaaatataaa
     3061 gctggaacct cttacccagt catttgggta gatcttcctt tgatggatat ttcttctagt
     3121 atgattaggg attttattaa aaaaggtcgt cagcccaatt atctcttacc taagcgggtt
     3181 ttagactaca ttactcaaga aggtttgtat caatgacata tgaagattat cttccttata
     3241 gcaggaccga actgttagca aaaattgcag agcagatgag ccctaaacgg tttaaacatg
     3301 tacttggcgt ggaaaaggca gccttgtcct tggcagagtg ttatggttgt aacccagaca
     3361 aagcagggtt agcagctctt ttacatgatt atgccaagga gtgccctgac caggtctttt
     3421 tagacttaat tgacaagtac caattatctc cagaattagc taaatggaat aataatgtct
     3481 ggcatggtat ggttggtatt tataagattc aagaagacct agggctaaaa gacaaagata
     3541 tcttgcgagc tattgaaatt cacactgttg gtgctgctga gatgaccttg ctggataagg
     3601 tcttatacgt tgctgattat attgaagaag gtcgcatttt tcctttggtt gatgacgcta
     3661 gaaaaatcgc gaagctcgat ttgaatcagg ctgttgccta tgaaacagtg aacactgtcg
     3721 cttatttagc ctcaaaagcg cagcctattt tcccacaaac tctggatacc tataatgcgt
     3781 tttgcagcta tttaaaggaa tactaatgga taatgatatg aggaataaaa tgaaaaaaga
     3841 agaactctta aaaattgttg tggaagcaac tgatgaaaag cgtgctaaag atattttaat
     3901 cttggattta gaggggttaa ctagtttgac tgattatttt gtgattgcga gtgcaacaaa
     3961 tagtcgtcaa ttagaggcta ttgcggataa tattcgtgaa aaggtaaaag aggctggtgg
     4021 agatgctagc catgttgaag gtaatagcca agcaggctgg gtcttgcttg atttgaccga
     4081 tgtggtggta catctttttt tagaagacga gcgttaccac tataatcttg aaaaattgtg
     4141 gcatgaagcg cctgctgtgg ctctagatgc ctatttagct taatgttaca aagagacctt
     4201 gtcaatacga cagggttttt gaggatgata atggaacata attatgaaaa atttgcgtca
     4261 gtttatgatg ctgtgatgga tgattctcta tatgacttgt ggacagattt ttcacttcga
     4321 catttaccaa agtctaaggg tcgaaatcgt ttgttagagt tggcctgtgg aaccggtatt
     4381 cagtcagttc gttttgctca agcaggtttt gatgtgactg gtttagactt aagtcaagac
     4441 atgctggcta ttgctaaaaa gcgtgcgcaa tctgctaaaa agaaaattga ttttattcaa
     4501 ggtaacatgc tggatttgtc acaggtgggg cagtttgatt ttgtaacctg ctattcggat
     4561 tccatttgct atatgcaaga cgaagttgat gttggcgatg tttttaaaga agtttatgat
     4621 gttttagcaa acgatggcat ctttattttt gatgtgcatt caacctatca aacggatgag
     4681 tgtttccctg gttattccta tcatgagaat gcagatgatt ttgcaatggt ttgggatacc
     4741 tatgctgacg aggctcctca ctctgttgtt cacgaactca ctttctttat ccaagaagac
     4801 gatggacgtt tttctcgctt tgatgaagtg catgaggaac gaacgtatga gttattgact
     4861 tatgatattt tgttggaaca agcagggttc aagtcattta aggtctatgc tgattttgaa
     4921 gataaggagc caactaagac cagcaagcgt tggttttttg tagcatataa atagaagaaa
     4981 aaagtagcct ttttagctat aggatttaat gaattatctt gaatgttact tatatgaaaa
     5041 gagttcatta ggcactaaat gctattatca aagcttagaa gtattggaga cattacgatt
     5101 gacactatgt ctgaaggttg ctaccaactt taaagaaagc attttctatt atacgttgtg
     5161 cttaagcatt tgatttaact cttattagcc ctataaaaga ttatttagta caatttgagg
     5221 agattctatg actgttaccg gaattatagc tgagtttaat ccgtttcaca atggacacaa
     5281 atatttgcta gaaacagcag agggactaaa aattattgct atgtcaggaa attttatgca
     5341 aagaggagag cctgctctta ttgataaatg gattcgatca gagatggctc ttaaaaatgg
     5401 tgcggatatt gttgtggaat tgcccttttt catttcagtt caatcagcag actattttgc
     5461 tcaaggagct attgatattc tttgtcaatt aggaattcag caattagcat ttgggacaga
     5521 gaatgtgatt gattatcaaa aactgattaa ggtctatgag aaaaaatcaa agcaaatgac
     5581 tgcctattta tcgacgcttg aagatacact tagctatcca caaaaaacac aaaaaatgtg
     5641 ggaaatcttt gctggagtta aattttcagg gcaaacgcca aatcatattt tggggttaag
     5701 ttacgcaaag gctagcgctg gaaagcatat acaactatgt cctatcaagc gtcaaggggc
     5761 tgcctaccat tcgaaagaca aaaatcatct gctagcttct gcaagtgcca ttagacagca
     5821 cctgaatgat tgggatttca taagtcattc agtaccaaat gcaggtttac ttataaacaa
     5881 tccccatatg tcatgggacc attatttttc attcttaaaa tatcaaattt tgaatcattc
     5941 tgatttaact agtatttttc aggtcaatga tgaattggcg agtcgtatta aaaaagcgat
     6001 aaaagttagt caaaatattg accacctagt tgacactgtt gctaccaaac gctatacaaa
     6061 agctcgtgta agacgtattt taacctacat tttggttaat gctaaggaac ctactttacc
     6121 taaggggatt catattctag ggtttacctc aaaaggacaa gcacatttaa aaaaattaaa
     6181 aaaaagcagg cctctgatta ctagaattgg agcagaaact tgggatgaga tgacacaaaa
     6241 agcagacagc atctatcaac ttgggcatca ggatatccca gaacaaagtt ttggaagaat
     6301 acctattata ataaaaaatg agaggctcaa ttaaactcta attgagtttt tttgggggct
     6361 tgactctaac attacgttga tggtttatac ttcagttaat ccaatgagga aattttataa
     6421 caataattaa aaaagtcagt caaaagatat gctttcgaag aacaggctaa cgtttttaca
     6481 agattatatc agtaatcatt ttgacaattg tagtaatgga atgttgccaa aatttagaat
     6541 gttgtgtgtt atgaatgaca agattttaaa ggaatactaa ttaaacggga ggtcgtgaac
     6601 cggaagctgt tgtcactcaa gcaccaaaat ctgtctgtaa cataatattg atgtatgaga
     6661 acgcaattat ttggttttta ttgtgatgct taaaacgatt ttatatttct attttaccaa
     6721 agcttttcaa agtgctataa taagggagtt agagtcaata ttagtaacgt taatgttgac
     6781 aatattcaat aaaatactat taaaaggaga caaaaatggg acgtaaatgg gcaaatattg
     6841 ttgctaaaaa aactgctaaa gatggagcaa catcaaaggt ctatgccaaa tttggtgttg
     6901 aaatttatgt ggctgccaaa caaggcgagc cagatccaga gttaaataca gctcttaaat
     6961 ttgtcattga tcgtgcaaag caagcacaag tgccaaagca tgttattgat aaagcgattg
     7021 ataaagctaa aggaaacaca gatgaaactt ttgtagaggg acgctatgaa ggttttggtc
     7081 caaatggttc aatgattatt gtggatactt tgacatcaaa tgttaaccgt acagcggcaa
     7141 atgtacgtac ggcttacggt aagaacggtg gcaatatggg agcttcagga tctgtatcct
     7201 acttatttga taaaaaaggg gtcatcgttt ttgctggtga tgatgctgac agtgtctttg
     7261 agcaattact tgaagcggat gtagacgtag atgatgttga agcagaagag ggaacaataa
     7321 cagtttatac cgccccaaca gatcttcata aaggtatcca agcacttcgc gataatggtg
     7381 tagaagaatt ccaagttact gaacttgaaa tgattcctca atcagaagta gtattggaag
     7441 gtgatgacct tgaaactttt gaaaagctta ttgatgcact cgaatcagat gatgatgttc
     7501 aaaaagtgta tcataatgta gcagattttt aaattgacat agaataataa aaagagttga
     7561 aatctatttc agctcttttt aagtgatgct tttataagta agtaccaggt aacatagtcg
     7621 ttgttagaag ataatgaaca atagtaaatc agtatcaact tgataggaat caattattag
     7681 tgaactctaa ttaaaaatgg taaactagac acaagaacaa aaaggagaga taggatgatt
     7741 aaaaaacaat atttaggaag agcaacaatt gccctagcat caacccttgt gctagcagct
     7801 tgtgggtcat caaaaacagc tgagtcagga aatcagggga gttctaaaga agtcttattt
     7861 gcaacggttg gaacaacagc tcccttttct tatgaaaaag gtggtcaact aactgggtac
     7921 gatatcgagg tggctaaagc agtctttaaa ggatcggatg attacaaggt gtcctttaaa
     7981 aaaacagaat ggtcttctat ctttacaggt ttggactcag gaaaatacca aatgggtggt
     8041 aacaacattt cctttaccaa agagcgttca gctaagtacc ttttttctta tccaatcgga
     8101 tcaacacctt ctgttttagt tgttccaaaa gacagtgata tcaagagctt tgatgatatc
     8161 caagggcata cgacacaagt tgttcaaggg actacaagtg ttgcacaatt agaagacttt
     8221 aataagaaac acagtgacaa cccggttact ctaaaattca ccaatgagaa cattactcaa
     8281 atgttaacca atttgagtga aggcaaggca gatttcaaga tttttgacgc tccaacggtt
     8341 aacgccatta ttaaaaatca agggttagat aatctcaaaa ccattgaact cacctcaaca
     8401 gagcaaccat ttatttattt tatctttagt caagatcaag agaaattgca aagctttgtt
     8461 aacaagcgta ttaaagagtt gactgctgac ggaactctta gcaagcttgc caaagagcac
     8521 cttggcggtg attacgtgcc atcagataaa gagttgaaac ttcctacagc taactaaacc
     8581 atctattaac cagttattca tctagaagac tggtttttct gatcggaaac aatattatag
     8641 tttatgaggt tatgttataa aaattgcaaa tagccttaat caaaatgaga tgttaaagtg
     8701 ctttttgaga agtgaatatt gtaagaagta tgctttgggt tttagtcctc aaataccaag
     8761 ttttttgttg actaaacaaa taaggatggt tagtatagca tatagtttaa aactatggta
     8821 tatgctagcc attatcaatt tgaaaactat atctaattaa gatacaataa tgatcgtaaa
     8881 caaaatgaat cgaggataac gctatgtctt ttaaaaaatg gattggaata gtcggaatcg
     8941 ctttagcatc aactgtattg gttgcttgtt ctgaaaaaca agatgataaa aacactctaa
     9001 ctattggggt gatgacaaaa acagaatctg atcaagcgag atgggataaa gttgaagaat
     9061 tattgaaaaa agacaatatc actttgaagt acaaagaatt tacagattac tcgcaaccta
     9121 ataaggcagt tgctaatggt gaagtcgata ttaatgcctt ccaacactat aatttcttaa
     9181 ataactggaa taaggaaaat aaggaacatt tggtggccat tgctgatacc tatatcagcc
     9241 caattaacct tttctctgga accagtcaag atgggaagac taaatacaaa tcagtagctg
     9301 atctgccaaa tggtgctcaa attgcagtac caaatgatgc gaccaatgaa agccgtgccc
     9361 tctatgtgct gcaatcagct ggcctcatta aattgaatgt gtctggtgat caattagcaa
     9421 ccattgccaa tatctcagag aacaaaaaga aattagatat caaggaatta gatgctagcc
     9481 aaacggcacg tgctttagta tctgctgatg cagctgttgt caataatagt tacgctgttc
     9541 ctgcaaaaat tgactacaaa acctctcttt tcaaagaaaa agcagatgac aattcaaaac
     9601 agtggattaa cattattgca ggtcaaaaag attgggaaaa atcagagaag gctgatgcta
     9661 ttaaaaaact catcaaggcc taccaaactg atgaggtgaa gaaagtcgtg gaaaaaactt
     9721 caaatggtat agatgtatcc gtatggtaaa ttttagccat tgttataaca agagtgtggt
     9781 cgtgtttaaa acacatgctt tcttgcatgc ttgaggaaga gtttacgact aatttacctt
     9841 tagaaagcta tttaagacct tttccctttt aggatttaga aaccttggga agactattgc
     9901 atgttatctt aatagtcatg attatgcagg cgatgaaaac attgctatcg tagattactg
     9961 tcgccatatc gtactaatag aggagttatt aaaatcttat gaatgaagca ataattcaat
    10021 tagatcatat tgatattacc ttccgtcaaa aaaaacgggt tatcgaagct gttaaagatg
    10081 tcacggttca tatcaatcaa ggagatattt atggcattgt tgggtattca ggtgccggga
    10141 aatcaactct tgtgcgtgtg attaacctgc tacaggcacc aacaaacggg aaaatcactg
    10201 ttgatgggga tgtgaccttt gaccagggga aaattcaatt gtcagccaat gcccttcgtc
    10261 aaaaacgacg tgatattggc atgatcttcc aacatttcaa tttgatggcc caaaaaacag
    10321 ccaaagaaaa tgtagcattt gcgctccgcc attcatcgtt aagtaagact gagaaagaac
    10381 ataaagttat cgagctgcta gaacttgtag gtttatcaga gcgagcagat aactaccctg
    10441 cccagttatc gggtgggcaa aaacaaaggg tggctattgc gcgtgctcta gctaacgatc
    10501 caaaaatttt gatttcagat gaggcaacat cagcactaga tccaaaaacc actaaacaaa
    10561 ttttggcgct cttgcaagaa ttaaaccgca aacttggcct taccattgtt atgattactc
    10621 atgaaatgca aattgttaag gatatttgta atcgtgttgc tgttatgcaa aatggtgttt
    10681 tgattgaaga aggttctgtc cttgatatct tttcaaatcc aaaagaagcc ttgacacaga
    10741 aatttatcac aacagctaca gggattgatg aggctttaga aaaaattaac caacaagaca
    10801 ttgtcaaaca tttgcctgca aatgcccttc tagctcagtt gaaatatgca ggaacttcta
    10861 cggatgagcc tctacttaat agcatttacc gtcagtttga agtgacagct aatattttgt
    10921 atggcaatat tgagatttta gatcatattc cagttggtga catgattgtt gtcttggaag
    10981 ggcaagctga aaatattctt gcggctgaga aagccttgca tgaagctggt gttgatgtta
    11041 gtattttaaa gagaggagcc tagatgtcac agttaattca aacttatttg ccaaatgttt
    11101 atgaactcgg atggtctgga gatgctggtt ggggcttagc gatttggaat actttataca
    11161 tgaccattgt gccttttatt gttgggggtg ctattggtct cttattaggc ttgttattag
    11221 ttttaacagg accggacggt gttattgaaa acaaaacgat ttgttgggtc attgataaag
    11281 ttacctctat ttttagagcc attccttttg ttattttgat tgctatttta gctagcttta
    11341 cctatttgct tttaagaaca actttagggg caacagcggc cttggtacct ttaacctttg
    11401 cgacttttcc tttttatgcc cgtcaggtcc aagttgtttt ttcagaatta gacaagggtg
    11461 ttattgaagc agcgcaagct tcaggtgcta ctttctggga tattgtcaaa gtgtatctta
    11521 gtgaaggttt accagatctt attcgtgttt ctacagtcac cttaatttct ttagttgggg
    11581 aaactgccat ggcaggagct atcggtgctg gaggattagg aaatgtagcg atttcttatg
    11641 gttataatcg ttttaacaat gatgtgactt gggtagcgac tattattatt cttttaatta
    11701 tttttgctat ccaatttatt ggtgatagct tgactagaag gtttagtcat aaataaacga
    11761 aacaacaccc taagttttta cttagggtgt tgttgtttag atgatgtcat tagcttttct
    11821 tcgttttact tagtataagc cctattaaaa aaccaatagc agcaaagata atccatccca
    11881 ttgagaattg ccctagtgga acagtttttt ggaaaaaagt tacaacggct tctggtaaat
    11941 gaaataatcc agtcatttgt gatagtgtta ggagtgcatc aaatatggca ggaataaagg
    12001 ttagaccaat tgtagttcga tagacaatag gatcattatt gaagcatttt tgtgccaaaa
    12061 caaggaaaat taaatcaact gttaatgggt ataaaaggaa taaaacagga gctgaccagt
    12121 tgataataac tgataaacca ccaaaataaa agaaagctga tagtaaagta aagatagttg
    12181 cccaagcaat atgagataaa gcaggcacta atttatggaa gtattcagca cttgacgtga
    12241 ttaagcctgt tgaagtggtt agacaggcca ggaaaatcac aacagctaga aatgcttgtc
    12301 cgatgccacc taggtaaaaa cgagaagcat gacttaagat ttgaccgcca ttaactggat
    12361 taccatgaag ggtaaagctg ccatcactaa aaggaaataa tgattgtgat gttgctccaa
    12421 tacgaccgac aaagatatag acaagtgcta atagcaaaat ggcaatagcc ccagaaataa
    12481 gtgttatttt ggtcacttct ttgtccgtct tagcgccaaa ttgtttggta gcctcaatga
    12541 ctaaaatagc aaaaacaaga gaagctagtg catccatagt gccataacct tgaattaatc
    12601 ctgctataaa aggaaaatct ttaaaggcat tattaacacc gaccccagcg ttaaaagcat
    12661 caccgtaatt tccagcagga tggacaaagg acgcgataac caaaatagaa ataacaacta
    12721 acaacgttgg cgttaaaaat tttccgatat tttcagctag cttactaggt ttgatagcaa
    12781 ggaagtagga cagaccaaaa aatagtatag cataaatagc tttattggtt gtattatctc
    12841 ctaagatagg agcgataccg actgaaaaag aagtggctcc tgttcttgga atagcaaaaa
    12901 atgggccaat agataaatac aaaatggaag aatagaagat tgcataccat ttagagatgg
    12961 ggcgtgccaa actttcaaca tcgcctgatc cagatttagc aacagcaatg acaccgagta
    13021 aaggcaagga gacaccagtt aaacaaaaac caatgataga ccatagaatc tgatgacctg
    13081 agtagatgcc taaaaaagct gggtaaatga ggtttgctgc tccaaaaaat agcgcaaata
    13141 gcatgaatcc aatgacgata tatacatttt tttgtttcat ttataaatag tctttctatt
    13201 gatactaatc cgaagattct aacaatttta attatattat gtgtcaaaat aacaacaaag
    13261 gttattatat ctaagattac ttcgatagtc aatagaaatt gactgaaaat tcttagcaca
    13321 aaaaatggtt aagatatgta
//
LOCUS       DAUGFP010000042        13337 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.042, whole genome shotgun sequence.
ACCESSION   DAUGFP010000042 DAUGFP010000000
VERSION     DAUGFP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13337
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.042"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            126..551
                     /gene="rplK"
                     /locus_tag="VH663_001295"
     CDS             126..551
                     /gene="rplK"
                     /locus_tag="VH663_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEQ8174493.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            657..1346
                     /gene="rplA"
                     /locus_tag="VH663_001296"
     CDS             657..1346
                     /gene="rplA"
                     /locus_tag="VH663_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEQ8174494.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            1670..2398
                     /locus_tag="VH663_001297"
     CDS             1670..2398
                     /locus_tag="VH663_001297"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEQ8174495.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            2427..2984
                     /gene="frr"
                     /locus_tag="VH663_001298"
     CDS             2427..2984
                     /gene="frr"
                     /locus_tag="VH663_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEQ8174496.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKATDDAIKEIDRMTAEKEKELLSV"
     gene            3093..3950
                     /locus_tag="VH663_001299"
     CDS             3093..3950
                     /locus_tag="VH663_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ8174497.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYIRLDVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            4023..4532
                     /gene="msrA"
                     /locus_tag="VH663_001300"
     CDS             4023..4532
                     /gene="msrA"
                     /locus_tag="VH663_001300"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEQ8174498.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPKQIAYKDLVELYWAQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPKRYAQSSAIR
                     HQFLEENWS"
     gene            4529..4744
                     /locus_tag="VH663_001301"
     CDS             4529..4744
                     /locus_tag="VH663_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEQ8174499.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            4900..6069
                     /locus_tag="VH663_001302"
     CDS             4900..6069
                     /locus_tag="VH663_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ8174500.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTEIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQVSATSQSTVPMAPSATPSDVPTTPFASAKPDSSVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDSTSANRP
                     VPNESASEEVSSAAPAQAPAEKEETSAPAAQKAVADTTSVATSNGLSYAPNHAYNPMN
                     AGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPENSALGDQVAQYA
                     IDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFNA"
     gene            6383..8155
                     /locus_tag="VH663_001303"
     CDS             6383..8155
                     /locus_tag="VH663_001303"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEQ8174501.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELIHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWIYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEGKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKDNETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            8314..9366
                     /locus_tag="VH663_001304"
     CDS             8314..9366
                     /locus_tag="VH663_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEQ8174502.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            9412..9987
                     /locus_tag="VH663_001305"
     CDS             9412..9987
                     /locus_tag="VH663_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEQ8174503.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            10146..10643
                     /gene="ybeY"
                     /locus_tag="VH663_001306"
     CDS             10146..10643
                     /gene="ybeY"
                     /locus_tag="VH663_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEQ8174504.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            10624..11031
                     /locus_tag="VH663_001307"
     CDS             10624..11031
                     /locus_tag="VH663_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEQ8174505.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            11151..12047
                     /gene="era"
                     /locus_tag="VH663_001308"
     CDS             11151..12047
                     /gene="era"
                     /locus_tag="VH663_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEQ8174506.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            12068..12544
                     /locus_tag="VH663_001309"
     CDS             12068..12544
                     /locus_tag="VH663_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ8174507.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
BASE COUNT         4174 a         2430 c         2873 g         3860 t
ORIGIN      
        1 tattttaaca aaaagacttg atttattttg gcaataagct ataatagtaa ggtatgcaat
       61 attgcatatc tgtgggaggt aaaaatctca attaccgcca aaaccacaac aggaggattt
      121 ttaaaatggc taaaaaagtc gaaaaacttg taaaacttca aatccctgct ggtaaagcta
      181 caccagctcc accagttgga ccagctcttg gtcaagcagg tatcaacatc atgggcttca
      241 ctaaagaatt taacgctcgt acagctgatc aagctggtat gatcatccca gttgttatct
      301 cagtttatga agacaaatca tttgatttca tcactaaaac accaccagct gctgttcttt
      361 tgaaaaaagc tgcaggtgtt gaaaaaggat caggtacacc taacactact aaggttgcga
      421 cagttactcg tgcacaagta caagaaattg ctgaaactaa gatgccagat ttgaacgctg
      481 caaacattga agctgcaatg cgtatgatcg aaggtactgc tcgttctatg ggattcactg
      541 ttactgacta gtcagtcaag aacccctatt acccgcacga cttcatcata agagatgagt
      601 gacgtgggag attttaaatc aaatcgatat gaccacatta caaggagaat tttaaaatgg
      661 ctaaaaaaag caaacaaatg cgtgctgcac ttgaaaaagt agatagcaca aaagcgtaca
      721 gtgtagaaga agctgtagca ttagtaaaag aaacgaactt cgcaaaattt gatgcgtctg
      781 ttgaagttgc ttacaacttg aacattgacg ttcgtaaagc agaccaacaa atccgtggcg
      841 caatggtatt gccaaacgga actggtaaaa cacaacgtgt tcttgttttt gcacgtggtg
      901 caaaagctga agaagcaaaa gcagctggtg cagacttcgt aggtgaagac gaccttgttg
      961 caaaaatcaa tggtggatgg cttgactttg acgttgttat cgcaacgcca gacatgatgg
     1021 ctatcgtagg tcgtcttgga cgtgtccttg gacctcgtaa cttgatgcca aaccctaaaa
     1081 ctggtacagt aacgatggat gttgctaaag ccgttgaaga gtctaaaggt ggtaaaatca
     1141 cttaccgtgc tgacaaagca ggtaatgttc aagctcttat tggtaaagtt tcatttgatg
     1201 ctgacaaatt ggttgaaaac ttcaaagcct tccacgatgt aatggctaaa gctaagcctg
     1261 caacagctaa aggaacttac atggcaaacg tctcaatcac atcaacacaa ggtgttggta
     1321 tcaaggttga tcctaactca ctttaagaaa aaaaattagg gcatcgccta atcatagcaa
     1381 aaagacaaat ttctgaagac aaatcttcac tagaggtttg tcttttttat ggtttaaaat
     1441 ggaatgcaaa caaaaaagtg taggcgataa taccatgaaa ggagttgatt ctcttaatga
     1501 gatgtggtgt taacacatca tgagctcaca aaaaatgata aaggaagtat cggtggtgtt
     1561 aaaaaaaggg attttaattg atatttatta gcctctaaaa tgtcctttcc ctattcataa
     1621 ttgagggttt gtggtaaaat agtaaggaca aactaaggag aacatagtag tggaacctaa
     1681 atatcaacgt atattaatta agttatctgg tgaagcatta gcgggtgaaa aaggagttgg
     1741 cattgacatt ccaactgttc aggctattgc aaaagaaatt gccgaagttc atgtgtcggg
     1801 agtacagatc gctcttgtta ttggtggagg caatctctgg cgtggagagc ctgcagcaga
     1861 cgctggaatg gaccgtgttc aggctgatta cactggcatg cttggaacgg tgatgaatgc
     1921 cctagtcatg gctgatagtc ttcaacatta tggtgttgat acccgtgtgc aaacagctat
     1981 tcctatgcaa aatgtagcag aaccttatat tcgcggacgt gccttgcgcc atctagaaaa
     2041 aaatcggatt gttgtttttg gagctggtat tggctcgcct tatttttcaa cagacaccac
     2101 cgcggcactg cgtgctgctg agattgaagc agatgctatt ttaatggcaa aaaatggtgt
     2161 ggatggggtc tataatgctg atcctaaaaa agatgctaat gcggttaaat ttgatgaatt
     2221 aacacatggt gaagtgatca aacgtggtct caaaatcatg gatgcaacgg catcgacctt
     2281 atcgatggat aacgatattg atttggtggt tttcaatatg aatgaagctg gcaatattca
     2341 acgtgttgtc tttggagaac atattggaac tactgtatca aataaagttt gtgactaaaa
     2401 gttagaaaag aaagagagta attatcatgg caaatgcaat tattgaaact gcaaaagaac
     2461 gttttgcaca atcccatcag tctttatcac gtgaatatgc cagcattcgt gcgggtcgtg
     2521 ccaacgctag tcttttagac cgtatccaag ttgattatta cggggcaccg acgccattaa
     2581 atcaattggc ttcaatcact gtgccagaag cgcgtgtctt attgatctcg ccttttgata
     2641 aatcttctat caaagatatc gagcgcgctc ttaacgcatc agatttaggc attacacctg
     2701 ctaacgatgg ctctgtcatt cgtttggtta ttccagcttt gaccgaagaa acacgtaaag
     2761 aattagccaa agaagtgaaa aaagttggtg aaaatgctaa aattgcaatc cgtaatatcc
     2821 gccgtgatgc tatggatgat gctaaaaagc aagaaaaagc aaaagaaatc actgaagatg
     2881 agttaaaaac acttgagaaa gatatccaaa aagcaaccga tgatgctatc aaagaaattg
     2941 accgcatgac ggctgaaaaa gaaaaagaat tgctctcagt gtaacaggat ttctacgaaa
     3001 aatagacgac tatagacaca aaaagatagg tgaccatttc agaagcgttt ggttttgatt
     3061 tgtccctatt tttttaaaga aagaaaaaag atatgaatga tttactagca acagtcatta
     3121 ctgggcttat taaagaagaa aacgctaatg actattttat ccaaaaagag gggtttacct
     3181 ttaccttgtc taaagcagaa ggagaacgcc agattggtga tatggtcaca ggctttgcct
     3241 atacagacat aaagcaaaag gcacgtctca caaccaaaga gatccgttca acacggacaa
     3301 gttatggctg gggggaagtg acagaggttc gtcgtgacct aggcgttttt gtcgatacag
     3361 gaattccaaa taaagaaatt gtggtttctt tggacgtgtt gcctgaaatg aaagaactat
     3421 ggcctaaaaa aggggataaa ctttacattc gtttggatgt ggataaaaaa gaccgtattt
     3481 ggggacttcc agcagagcct gaggtctttc aaaagatggc tagccctgca tacaataata
     3541 tgcaaaatca acattggcca gcaattgttt accgcctaaa attaacagga acgtttgttt
     3601 acctgcctga aaacaacatg ttaggcttta ttcacccaag tgaacgctac gcagaaccgc
     3661 gcttaggtca ggttttagat gcccgtgtga ttgggtttag agaggtcgat cgtaccctaa
     3721 acttgtcgtt gaaaccacgt tcttttgaga tgttggagaa tgacgctcag atgattgtca
     3781 cctatcttga agccaatggt ggtttcatga ccttaaatga caaatcaagt ccagaagaga
     3841 ttaaagcaac ctttggtatt tcaaaaggcc agtttaaaaa agccttgggt ggattgatga
     3901 aagctaaacg catcaaacaa gacgccacag gaactgaatt aataggataa aaatgctttg
     3961 ccactggcaa agttttttgt tacaatgaag accattaact gaagaaaaag gggatataga
     4021 gaatggaaag agccattttt gcaggaggtt gtttctggtg tatggtgcaa ccttttgagg
     4081 agcaagcagg tattttgtca gtcagaagtg gctacacagg tgggcatctc ccaaatccta
     4141 gctatgaaca agtttgtgcc aaaacaacag gacatacaga agctgttgaa attatttttg
     4201 accctaagca aatcgcttat aaagatttgg tagagctcta ttgggcgcag acggatccaa
     4261 cagacgcctt tgggcaattt gaagatcgtg gggacaacta ccgtcctgtc atttactata
     4321 caacggaaag acaaaaagaa attgcagagc aatcaaaagc aaacttgcaa gcttcaggac
     4381 gttttgacca acccattgtc accactattg aaccagcaga gccattttat ctcgcagagg
     4441 attatcacca aggattttat aaaaagaatc ccaagcggta tgcacaaagc agtgctattc
     4501 gtcatcagtt tttagaggag aattggtcat gagaaaatca ttttacagct ggctgatgac
     4561 acaacgtaac cctaaatcaa atgaacctgt ggcgatttta gcagatttgg tgtttgatga
     4621 caccactttt ccaaagcata ccaatgattt tgaactcatt agtcggtatc tagaagatca
     4681 agctagtttt tcctttaatt taggtcaatt tgatgaaatc tgggaagatt atctagcaca
     4741 ttaatacgtg gataaaaaag agtcatatcg actcttttga agggttatca cgttattatt
     4801 acaaaatgac atcactttct ttcaaaaaaa tgacaagcta ctttttttct taaaaatatg
     4861 ctatgatata gcttagaaaa aaataaaaag gaagcagaga tgattattac taaaaagagc
     4921 ttatttgtga caagtgtcgc tttgtcgtta gcacctttgg cgacagcaca ggcacaagag
     4981 tggacaccac gatcggttac agaaatcaag tctgaactcg tcctagttga taatgttttt
     5041 acttatactg taaaatacgg tgacacttta agcacaattg ctgaagcaat gggaattgat
     5101 gtgcatgtct taggagatat taatcatatt gctaatattg acttaatttt tccagacacg
     5161 atcctaacag ccaactacaa ccaacacggt caggcaacga ctttgacggt tcaagcgcct
     5221 gcttctagtc cagctagcgt tagtcatgta cctagcagtg agccattacc ccaagtatct
     5281 gccacctctc aatcgactgt tcctatggca ccatctgcga caccatctga tgtcccaacg
     5341 acaccattcg catctgcaaa gccagatagt tctgtgacag cgtcatctga gctcacatcg
     5401 tcaacgaatg atgtttcgac tgagttgtct agcgaatcac aaaagcagcc agaagtacca
     5461 caagaagcag ttccaactcc taaagcagct gaaacgactg aagtcgaacc taagacagac
     5521 atctcagaag attcaacttc agctaatagg cctgtaccta acgagagtgc ttcagaagaa
     5581 gtttcttctg cggccccagc acaagcccca gcagaaaaag aagaaacctc tgcgccagca
     5641 gcacaaaaag ctgtagctga caccacaagt gttgcaacct caaatggcct ttcttacgct
     5701 ccaaaccatg cctacaatcc aatgaatgca gggcttcaac cacaaacagc agccttcaaa
     5761 gaagaagtgg cttctgcctt tggtattacg tcatttagtg gttaccgtcc aggtgatcca
     5821 ggagatcatg gtaaaggttt ggccattgat tttatggtgc ctgaaaattc tgctcttggt
     5881 gatcaagttg ctcaatatgc cattgaccat atggcagagc gtggtatttc atacgttatt
     5941 tggaaacagc gattctatgc gccatttgca agtatttacg gaccagccta cacatggaac
     6001 cccatgccag atcgcggcag tattacagaa aaccattatg atcatgttca tgtctccttt
     6061 aatgcttaat tcatgctatc aaaaaaactc acgtttaggc gtgagttttt tgacaattct
     6121 gacagagtaa gccctataag aggctgagat acgcttttaa tacctgttgt ttttctcacc
     6181 ctttagacag ttttaacgtt atgtctaaaa gagatggttt ttattagcgc aaatatgggt
     6241 ttaggggtaa aataaaggta attaacctga tatgatcatg ataaaggtag aagaatacct
     6301 attatttcgt gacaactgag caatctgcct tacctttgga gtgatgatat cactttgctt
     6361 gactattcaa ggaggtcttg aaatgtatta tactagtggt aattacgaag cttttgcaac
     6421 acctcgaaaa cctgaagggg tagatcagaa atcagcttat attgttggta ctggtttagc
     6481 tggtttagca gcagctgttt tccttattcg cgatgggcat atggctgggg aacgcattca
     6541 tctgtttgag gaattgcctt tagcaggtgg ttctttagat ggtattgaaa agcctcatct
     6601 tggttttgtg acccgtggtg gtcgtgagat ggaaaatcat tttgagtgta tgtgggacat
     6661 gtatcggtct attccctcac tggaaattcc tggtgcgtct tatttggatg aattttattg
     6721 gttggataag gatgatccta actcatccaa ctgtcgtttg attcacaaga gaggaaatcg
     6781 tgtggatgat gacggccagt atacgctcgg taaacagtca aaagaattaa tccatttaat
     6841 catgaagaca gaagaatctc taggagatca aaccattgaa gagttcttct cagaagattt
     6901 ctttaagagt aatttttgga tctattgggc aaccatgttt gcttttgaaa aatggcattc
     6961 tgctgtagaa atgcggcgct atgcgatgag gtttatccac catattgatg gtttgccaga
     7021 tttcacctct ctcaagttca acaaatataa ccaatatgat tctatggtca aaccgattat
     7081 tgcttaccta gaatcacacg acgttgacat ccaatttgac acaaaagtca ctgatattca
     7141 ggtagaacaa acagctggta aaaaggtagc aaaaaccatc catatgacgg tgtctgggga
     7201 gggtaaggcg attgagctaa cacctgatga tttggttttt gtgaccaatg gttctattac
     7261 tgaaagtagc acatacggta gtcatcacga agtggctaag ccaaccaaag cgttaggtgg
     7321 ttcttggaat ttatgggaaa atctagctgc tcaatcagat gattttggtc atcctaaagt
     7381 gttttaccag gacttgcctg ctgaaagctg gtttgtgtct gccacagcaa ccataaaaca
     7441 cccagctatc gagccttata ttgaacgttt gacccaccgt gatttgcacg atggcaaagt
     7501 gaacactggc ggcatcatca ctattacaga ttccaactgg atgatgagct ttgctattca
     7561 ccgtcaacct cattttaaag aacaaaaaga taatgaaacc actgtctgga tttacggtct
     7621 ttattccaat agtgagggca attacgtcca caagaaaatt gaggagtgta caggtcaaga
     7681 aatcacagaa gaatggttgt accaccttgg ggtacctgtt gataaaatca aggacttagc
     7741 gagtcaggac tatatcaata cagttcctgt ttacatgcct tatattacga gttactttat
     7801 gccacgcgtc aaaggagacc gtccgaaagt tatcccagat ggttcagtca acttggcctt
     7861 tattggtaac tttgcggaat ctccatctcg agatacggtc tttacgactg agtattctat
     7921 tcgtactgcc atggaagcag tgtatagctt cttgaatgtg gaacgaggca ttccagaagt
     7981 ctttaattca gcctatgata ttcgtgaatt gctcaaagcc ttttattacc ttaatgataa
     8041 aaaggcaatc aaggatatgg atttgccaat tcctgcactg attgagaaaa tcggacataa
     8101 aaaaatcaag gataccttta tcgaagaatt gctcaaagat gctaatctta tgtaacatgc
     8161 tttagagtag ttaaagctga taagtttagg cttatcagct tttttgatga tttatagaaa
     8221 agtgataaag gaaaagtctg acgtgaacgt cacataagaa tttaaaattt gttataatgt
     8281 aagcaataaa gatgaaagaa agagggactc tatttgcaag agtattctat tgacattacc
     8341 ttaacccatc ctgatgatgt tttagctctt tttggtagca acgagcgtca tttgaagcta
     8401 atagaagctc atttaggagt tattgttcac gcaagaaccg agcgagtcca agtgattggt
     8461 gatgatgaag aagctgtgga attggctcga ttgaccatca aagccctact ggttctagtg
     8521 ggtcgtggca tggtggtcaa cacatcagat gtggtgacag ctttatctat ggcagaatct
     8581 catcagattg accaatttat ggcgctttat gaagaagaaa tcattaaaga taactatggt
     8641 aaggcaatac gagtcaaaac attaggtcaa aaaacctatg ttgatagtgt caaacgtcat
     8701 gatgtggttt ttggtgtagg acctgcaggg acagggaaaa ccttcttagc tgttacgctt
     8761 gcagtcactg ctctgaaaag aggacaggta aagcgtatta ttttgacgcg tcctgcggta
     8821 gaagctggtg aaagcctagg ttttttacca ggcgatttga aagaaaaggt ggatccttac
     8881 ctcagaccgg tttacgatgc cctttatcac attttaggca aggaacaaac cacacgcctg
     8941 atggaacgag atgtgattga gattgctccg ttagcttata tgcggggtcg tactctggat
     9001 gacgcttttg ttatcttaga tgaggctcaa aacacgacta tcatgcaaat gaagatgttt
     9061 ttgacccgtc ttggttttaa ttctaaaatg attgtcaatg gagatacgag tcagattgac
     9121 ctccctcgaa atgtcaagtc tggtttgatt gatgctactc agaaattaca aggcattaaa
     9181 cagattgatt ttgtctattt ctctgctaaa gatgttgtcc gacatcctgt tgtggctgat
     9241 attatcaagg cttatgaaac gtcctcagag gagatgaagg acttactaaa acctaaggct
     9301 agtgacgttg aagctgacta ccagccaggc ttgacaaaat accctgtgat cggtcaagag
     9361 cactaagacc tttacgatga tgtttaatat ggccttttta aggaggattc tatggatgat
     9421 ttaaccaaag ctattatggc agatgaggct aatctctcct ataccgaaag aggtattttt
     9481 cctttatatg atgctcctca aacagctagg attattattg ttggccaagc tcctggaata
     9541 gtagctcaag aaaccaaact ttactggaat gatcgtagtg gcatccgctt acgagactgg
     9601 ctaggtgtgg acaatgacac cttttaccac tcaggattgt ttggcattat ccctatggat
     9661 ttctattatc ctggtaaggg aaaatccggt gacttaccac ctagagaagg ttttgcggct
     9721 aagtggcacc cgccattaag agcattgatg ccagaagttg aattgacgat tttggttggt
     9781 cgctatgctc aggactttta ccttggtaac aaggcttaca aaaccttaac tgaaacagtc
     9841 cgtcactttg aggattactt gccagactat tttccacttg ttcatccttc tccaagaaac
     9901 caattgtggt tagctaagaa cccctggttt gagcaggatt tattacctat cttgcaaaaa
     9961 cgtgtggagg caattctaac caaataggcc ctgatttacc tagcattccc atttttaagg
    10021 ggcttgtggt ataatgggta aataacaaat gctgattgaa aggataaggg aaattatctg
    10081 tcagataata atgggtgccc aacataccaa atgaggtggg cagcccttcc ctaacaatta
    10141 accttatgta tatcgagatg attgacgaaa cgggacaagt ttcgcaagag attatggagc
    10201 aaacacttga tttgctcaat tttgctgctc aaaagacggg caaagaagaa aaagaaatgt
    10261 ctgtgacctt tgtgaccaat gaaagaagtc atgaactcaa cttagaatac cgagatactg
    10321 atcgtccaac agatgtgatt tctctagaat ataagccaga aacgcctatt ttatttagtc
    10381 aagaggattt ggctgctgat cctagtttgg cagagatgat ggcagagttt gatgcttata
    10441 ttggcgaatt gtttatttct attgacaagg cgcgtgagca gtcccaagaa tatgggcact
    10501 cttttgagcg tgaaatggga tttttagctg ttcatggatt cttacatatt aatgggtatg
    10561 atcactacac ccttgaagaa gaaaaagaga tgtttacctt acaggaagag attttgactg
    10621 cttatggcct tacacgacaa taataccacc aaacggaaat ggaaaaacag aaccattacc
    10681 tcaagcctgg agtttgcctt gacaggggtt ttcacagctt ttaaggaaga acgcaatctc
    10741 agaagtcacc ttttgtctgc ctgtttagct tgtgttgctg gtttattttt tagcatttca
    10801 gccatcgaat ggttattttt acttctagcc atttttttgg tcattacctt agaaattgtc
    10861 aattctgcta ttgaaaatgt agttgatttg gctagtgact atcacttttc tatgctagct
    10921 aaaaatgcta aggatatggc agcaggtgct gttttgatga tttctggcta tgctgtcttg
    10981 acaggactta ttatttttat tcctaaaatt tggaatattt tcgttcatta aaaagaaagg
    11041 aatgggctat cctatgtgat gatggcatag gagaataagg aaaggacaag acggttaagc
    11101 ttagtgacaa gcttgatctg aagccctacc gttaaagccc gtaattatct atgtttaaat
    11161 caggctttgt agccattctt ggtcgtccaa atgttggcaa atccaccttt ttaaatcacg
    11221 tcatggggca aaaaattgcc atcatgagtg acaaggctca gaccacccgt aataaaatta
    11281 tgggcattta caccacagaa acagaacaaa ttgtctttat tgatacgcca ggtattcata
    11341 aaccaaagac cgcccttggt gattttatgg ttgaatctgc ttatagtact ttacgtgaag
    11401 ttgagacggt tcttttcatg gttcctgccg atgaaaagcg tggtaagggt gacgacatga
    11461 ttatcgagcg gctaaaagca gctaagattc ccgttatttt agtcatcaac aaaattgata
    11521 aggttcatcc agaccaactt ttggaacaga ttgatgactt ccgtagccaa atggatttta
    11581 aagaagtggt cccaatttct gccctagaag gtaataatgt gccaacccta atcaagttat
    11641 taactgataa tcttgaagaa ggtttccaat attttccaga agatcagatt actgatcatc
    11701 cagaacgttt ccttgtgtca gaaatggttc gtgagaaggt actgcatttg acgcaacaag
    11761 aggttccaca ttccgtagct gttgttgtcg aatccatgaa gcgagatgaa gagactgata
    11821 aagtgcatat ccgtgcaacc attatggtgg agcgcgatag ccaaaaaggc atcatcattg
    11881 gaaaacaagg agccatgctc aaaaaaatcg gaaaaatggc gcgccgtgat attgaactca
    11941 tgctaggtga caaggtctac ttagaaacgt gggtcaaagt caagaaaaac tggcgggata
    12001 aaaagcttga ccttgctgac tttggttata atgagaaaga atactaacat agtaaagcga
    12061 gcatcatatg ccacaagact acatttctta tatccgctcc aaagttgggc atgataagat
    12121 tattcttaac tttgctggtg ggattttgac caatgatgac ggcaaggtgc tgatgcagtt
    12181 gcgtggtgat aagaaaacct gggctattcc tggtggcact atggaactgg gtgagagctc
    12241 tttagagact tgcaaacgtg agttcttaga ggaaaccgga attgaggttg aagctgttcg
    12301 cttactgaat gtttacactc attttgagga agtttatccc aacggtgatg ctgttcagac
    12361 tattgtcttt atctatgaac taacggctgt ttctgatatg gctattgata actttcataa
    12421 tgaggaaacg ctcaagttac agtttttctc tcatgaggag atagcagagt tagagagtgt
    12481 ctcagccaaa catcgcctga tgttagagga atattttagt gatagctttg ctatggggca
    12541 ttaagaactc tctaggacag ttggagtatt tttggagatt ggcttgttaa aaaaattttt
    12601 gaaagacgat gtttcgagag cttatgctct ctttttttgc ttccaatttt tctgattaag
    12661 tctgaataat tttgttaaat gttaacagga ttttctttcc aattgatact agcagacatc
    12721 aaacatttgt tttagagtta tgacgaattg ccacctagcc atgcatgact taggcaagtt
    12781 gaacctaact gtggcaatag acttggaagg ggtgacaaga atcatttcct aaaagaattg
    12841 atgaagtgcc tatccattat tcgaattgta aagttcgtct aagtaaagtt gaatttgatc
    12901 tcgaatggac tttaatgatt tatgacttgt tgtgtcaata acaccaaaaa ctcgataatg
    12961 atttttaaat atttcctcgt aattaatatg atttccgatt agaataatat gagcatgagg
    13021 ataacgcata cgtatttcgt ttagaaggtc atatacttgc ttagatttaa aaaagtatga
    13081 tttatgataa aataaacctg acatttgtat ttataatcta agtttttccc actaataatt
    13141 taagttttta gaaagtatct cttgaggtaa tagttaatat agatagatat catatatcct
    13201 aaaagagaac gatgtcttgt aagtattttc gaagactata atattaagtt cggagctcgt
    13261 attcataaag gattcggagt atattttgac acacttgata gcttatttga ctaatattca
    13321 aaaactctac tcttgtg
//
LOCUS       DAUGFP010000043        13325 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.043, whole genome shotgun sequence.
ACCESSION   DAUGFP010000043 DAUGFP010000000
VERSION     DAUGFP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13325
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.043"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            185..313
                     /locus_tag="VH663_001310"
     CDS             185..313
                     /locus_tag="VH663_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEQ8174508.1"
                     /translation="MAFGENGPRKKTTFEKVTMVVVILMVLVTVGGLIASALSVLM"
     gene            370..1683
                     /gene="obgE"
                     /locus_tag="VH663_001311"
     CDS             370..1683
                     /gene="obgE"
                     /locus_tag="VH663_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEQ8174509.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVDEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDESDLVDEEAYYGFAEAEKEFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDLVRIGKFEFEFVD"
     gene            1752..1910
                     /locus_tag="VH663_001312"
     CDS             1752..1910
                     /locus_tag="VH663_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174510.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(1915..2610)
                     /locus_tag="VH663_001313"
     CDS             complement(1915..2610)
                     /locus_tag="VH663_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEQ8174511.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDASQYAVVICFATFLINDSYTFYSWL
                     MLQRYQEKKVKKG"
     gene            2835..4235
                     /locus_tag="VH663_001314"
     CDS             2835..4235
                     /locus_tag="VH663_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ8174512.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSDAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEIIKNNTVDFLGVNYYQPLRVQAPSKSQQEGNPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            4244..5338
                     /locus_tag="VH663_001315"
     CDS             4244..5338
                     /locus_tag="VH663_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEQ8174513.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAERSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLAEGLPTVEDHRHLPIGLQVELMKAIGT
                     IDNILISNQFISEEELAACTQALARPVTTIKVRPIIDLTEVEEQIIGYPHCYRGDVSD
                     YVIRSTMPRLVYAQESIAPRDQSKEVKRGCIIIDNDRYHRYKGELQIALKNFTVSSKA
                     NVVAEVREDYLSLLDDLRPWQEFCLEIDPS"
     gene            5463..7439
                     /locus_tag="VH663_001316"
     CDS             5463..7439
                     /locus_tag="VH663_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ8174514.1"
                     /translation="MAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKELISSLPE
                     HGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVSQVEESVDREDYVLHKFF
                     FEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGKEEDIRH
                     FIMDYFFVTSFDNTLSTMVGNIFLEGINFAEIMIIVLDECRDAKLKLSDFVMNNLVLH
                     IALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEANYIALH
                     LKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAHMMPLTT
                     RLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISLHLMAAI
                     ERYSNRHKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYEITEERLKTVDL
                     IISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMSLAKAEQ
                     LLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTEMFVEDFYHQMVLRENYSPVIY
                     GEVLAFPHPANPMTYSEQVVVAICREPLEWDKVHQAVHFVFLLSPSKGHNHRLKYVSP
                     GLASFVNQVELQQTLLEEPNYSKFMTVFTPLIHD"
     gene            7462..7773
                     /locus_tag="VH663_001317"
     CDS             7462..7773
                     /locus_tag="VH663_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ8174515.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            7776..8111
                     /locus_tag="VH663_001318"
     CDS             7776..8111
                     /locus_tag="VH663_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ8174516.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRCES"
     gene            8108..8557
                     /locus_tag="VH663_001319"
     CDS             8108..8557
                     /locus_tag="VH663_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEQ8174517.1"
                     /translation="MIISAEGDVSIHHCFEAMLISLQEDYRQNTLRRLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYRLEALGENRTRITYSYD
                     YHPLDIFQKANHFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            8735..10114
                     /locus_tag="VH663_001320"
     CDS             8735..10114
                     /locus_tag="VH663_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ8174518.1"
                     /translation="MSHFSDKFMDISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPGLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            10111..10263
                     /locus_tag="VH663_001321"
     CDS             10111..10263
                     /locus_tag="VH663_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174519.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYLHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(10411..11151)
                     /locus_tag="VH663_001322"
     CDS             complement(10411..11151)
                     /locus_tag="VH663_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174520.1"
                     /translation="MTELKIDVQGLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVMVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEAAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(11151..13325)
                     /locus_tag="VH663_001323"
     CDS             complement(11151..13325)
                     /locus_tag="VH663_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ8174521.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGNFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATISDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDVVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
BASE COUNT         3997 a         2424 c         2863 g         4041 t
ORIGIN      
        1 cagacgagga ggtttttatc tgtaacatta ttttgcaaat gctaatgtat catattcata
       61 tgaaattcac aagatacaat actcagtaat agcctacata gcgtaaatat tcatctggta
      121 tttgcctaaa acgatctttc atgttaagat ataagaagaa aaatcaatag atgaggtatt
      181 taacgtggca tttggagaaa atggacctcg caaaaaaaca acctttgaaa aagtgacaat
      241 ggtcgttgtt atcctaatgg ttcttgtaac tgttggcggg cttattgcga gtgccctatc
      301 agtattaatg taaggaaacg ctgaaaggcg tttttttaaa gtaaaagaaa gttagagaat
      361 aaaaaaagta tgagtatgtt tttagatacg gcaaaaatca gtgtccaagc aggccgtggt
      421 ggcgatggta tggtagcttt tcgtcgtgaa aagtacgtcc caaatggcgg tccttgggga
      481 ggagacggcg gtaaaggtgg ctcagttatc ttccgagtgg acgaaggttt gcgtaccttg
      541 atggatttcc gctataatcg taaattcaag gcgaaatctg gtgaaaaagg tatgaccaaa
      601 ggcatgcacg gccgtggtgc agaagatttg attgtttttg ttcctcaagg gacaacggtt
      661 cgtgatgctg aaactggaaa agtaattaca gacttagtag aacatggtca agaagtggtc
      721 attgctaagg gtggtcgtgg tggccgtggg aatatccgtt ttgcaactcc tcgtaatcct
      781 gcgccagaga ttgctgaaaa tggtgagcca ggtgaggaac gtcagttaga gttagaactc
      841 aaaatcttag ctgacgttgg cttggtcgga ttcccatctg ttgggaaatc aaccttgtta
      901 agtgttgtgt catcagctaa accgaaaatt ggtgcctatc attttaccac tattgttcct
      961 aatcttggaa tggttcgtac aaagtcaggt gatagctttg ctatggcaga tctgccaggt
     1021 ttgattgaag gtgctagcca aggggttggt ttgggaactc aattcctccg tcatatcgag
     1081 cggacacggg ttattcttca tgtgattgac atgtctgcta gtgagggtcg tgatccttac
     1141 gaagattatg tttctattaa taacgagctg gaaacctata atttgcgatt gatggagcgc
     1201 ccacagatta ttgtggctaa taaaatggac atgccagagg ctcaagaaaa cttgaaagcc
     1261 ttcaagaaaa aattggctgc ccaatatgac gagtttgatg atttgccaat gattttccct
     1321 atttcaagcc tagcacatca aggcttagag aacttgttag aagcaacggc agaattactt
     1381 gctaagacgg atgaattctt attgtatgat gaatctgatc tggtggacga ggaagcttac
     1441 tatggttttg ctgaggctga gaaagaattt gagatcacac gtgacgatga cgctacttgg
     1501 gtcttgtctg gtgagaagtt agaacgtctc tttgtcatga ccaatatgga acgtgacgaa
     1561 tccatcatga aatttgcccg tcaattgcgc ggtatggggg ttgacgaagc ccttcgtgaa
     1621 cgtggagcaa aagacgggga tcttgttcgt attggtaaat ttgaatttga atttgtggat
     1681 taatgtggtc tttagaaatg gcattgccat agcagccttg tgttttatct ttaagatgga
     1741 ggtttctgat tatgggtgat aaaccgatat cctttaagga taaggatgga aattttgtat
     1801 cggcagcaga tgtttggaat gctgagaaat tagaagaatt gtttaatctt ttaaatccca
     1861 atagacgttt gcgcttagaa cgtgaaaaat tgaaaaagga tgaggcttag gccctcatcc
     1921 ttttttgacc tttttttctt ggtaacgttg gagcattagc caactataaa aagtgtaact
     1981 atcattaata agaaaggtgg caaagcagat gaccacagca tattggctgg catctgtttg
     2041 agcagcatgc atccataaga gaatgaggac tagatcattg agtccataag ccaaagcaaa
     2101 ataagggctt cgcttatagc tgagataaac ggctgagaaa gaagtggcaa tcgataaagt
     2161 ggagacaagt aaataggctg tttgaataga agccaagatg ccgtagaaaa taagagtgac
     2221 cagtatagta aaaacgaaaa gacatcttcg atcagtgggt gttaatcgtg aaatagtcac
     2281 ttgtgatttt ttcccttcaa aaggatggtt taaccaagta aaaagtgaaa aaatagtcat
     2341 agggagggtc atgaaaagat aggtcattaa ctctccatag taactgtttc gtaatgaaag
     2401 ataggcatag ataatggaaa aaataatgac gagcccttga ccaataggat tagctttagc
     2461 actaaaaatg agagaggtca caccaatcaa agaagctatt aaagctaagg gagcttgctt
     2521 accgaataaa agggcgctaa ttaaaatggc caagacagag cttaaccaaa gtgcccactc
     2581 ggtttttgta aagtagttta aataagccat acccctattc aaacataggc taaggcatct
     2641 gtcaataggg gcttggtttt gataagaagt agcagggcag gttagatagg ctctttaaga
     2701 taataaaata tgtcaagctt taaatcactt attttccgtg atgataagga aaaaacagat
     2761 tttttaaagc gtttacatca cctgtttttc ttcttataat aaaaagagaa gtgagcaaag
     2821 aaaggacaaa ggatatgaaa caaagaaaac accgttatca atttccagat ggctttttat
     2881 ggggaagttc cacatctggg cctcaaagcg aaggaacggt tcctggagat ggcaaaggac
     2941 ctagcaactg ggattactgg tttagtattg agtcagctaa gtttcatcac caaattggtc
     3001 cagaaaaaac ctcaaccttt tatgaaaact ataagggtga cattgccctt ttgaaagaaa
     3061 caggccacac gatttttcgg acctcaatcc agtggtctcg attgattcca gaaggagtcg
     3121 gggaggttaa tccaaaggcg gtgacatttt accgagaagt atttcaagac attattgctc
     3181 aaggtattaa attgattgtt aacctctatc attttgacct cccctatgcc ttgcaagaaa
     3241 aaggtggctg ggaaaacaaa gctactgtct gggcttatga aacctacgca aaaacctgtt
     3301 ttgagctttt tggtgattta gtaaacactt ggattacctt taatgaaccg attgttccag
     3361 tcgaatgtgg ctatttaggt tattatcact acccttgtaa agtagatgcc aaggcagcag
     3421 ttcaagtggc ttacaacaca caactggcca gttcactggc ggtcaaagct tgtcataagc
     3481 ttcatcccga ccacaagatt agtattgtgc ttaacatgac acctgcttat cctcggagtg
     3541 atgctcctga agatgtcaag gctgctagaa tcgcagagct tttccaaacc aaatccttcc
     3601 tagatccatc agttttgggt gtttacccag cagagttagt ttccatttta gaggaagctg
     3661 atttattacc tcaatatagt gctgatgaat tagagattat caaaaacaat actgttgatt
     3721 ttttgggagt taattattat cagcctttgc gggttcaggc acctagtaag tcacagcaag
     3781 aggggaaccc ccttatctta gatatttatt ttgaacctta tgatatgcct ggtaaaaaag
     3841 tcaatcctca ccggggttgg gaaatttatg aaccaggact ttatgatatt gcccttaatt
     3901 taaaagaaca ctatggtaac atcgaatggc tagtcacgga aaatggtatg ggtgtcgaag
     3961 gtgaagaagc ttttttagca gatggccaga tacaagatga ctaccgcatc acctttatcg
     4021 aggatcatct tatccagctt cataaggctt tgggagaagg agccaactgc aaaggctacc
     4081 tcttgtggac ttttattgat tgctggtctt ggttaaatgc ttataaaaac cgatatggtt
     4141 tggtagcttt ggatttagag agtcaaaagc gaaccataaa aaaatcaggt tactggttta
     4201 aggcgttgag cgagtcaaat ggttttgata aataggaggc aatatggttg atttaggatt
     4261 ttctctatac cctgagcggt atgatgttac caagagtaaa gcttatattg acttatgtca
     4321 tagctatgga gccaagcgct tgtttatgag cctcttacag ctggcaccag ctgatcacca
     4381 gatgtttcat tgctacgcag agctcattgc ttatgcaaat caattaggca ttcgagtgat
     4441 tgctgacgtt agtccaagct ttattagcca agctggttgg tcagatcagc ttattgaacg
     4501 agctcatgcc ttcgggttag cgggcttacg cttagacgag gctctccctc tagcagagat
     4561 tgtaacttta acaagaaatc cctttgggct taaaattgaa cttaatatga gcactgataa
     4621 acaattattg atgtcattgc tagcgacaga tgcagaacgg agcaatatta ttggttgcca
     4681 taatttttat ccacatgaat ttaccggttt gtcatggcaa cattttaagg atatgtcacg
     4741 tttttatcat gagcatgata ttgaaacggc tgcttttatt accgctcagt cagcatctga
     4801 aggcccttgg ctactggcag aaggcttgcc gacagtggaa gaccataggc acttgcccat
     4861 tggcctgcaa gtagaattaa tgaaagcgat aggaaccatt gataatatcc tcatatctaa
     4921 ccaattcatt tcggaagaag aattggcagc atgtactcag gctttagcaa gaccagtcac
     4981 aacgatcaag gttaggccta tcatcgattt gacagaagtt gaggagcaaa tcattggcta
     5041 tccgcattgt tatcgtggcg atgtatctga ttatgtgatt cgatcaacca tgccacgtct
     5101 agtttatgct caggaatcga ttgctccaag agaccaatct aaggaggtca aacgaggttg
     5161 cattattatt gacaatgacc gctatcatcg atataaggga gaactgcaaa ttgctttgaa
     5221 aaattttacg gttagttcaa aagctaatgt ggttgctgag gtcagagagg actatcttag
     5281 tttgttagat gatttgcgac cttggcagga attttgtttg gagattgacc catcatagat
     5341 tcttttcaca atcaatgcgg aaaatgatgg tcaccttttt caggaaatat ttggtacact
     5401 aaaagagggt agaaagcgtt ttctaaagta gtaaggagga ctaatgttat caaaaaaaag
     5461 aactggccat cataacattt ctaatccatc acaaagagca gtttgtctct agtgcaactc
     5521 tggctgaggt tattggtatg agtgaccgta cggttaggaa atacctcaaa gaactgatca
     5581 gtagccttcc tgaacacgga gctcacatca tttctaaaca aggtcgaggt tactgcttag
     5641 aaattgacca ttctatggca tttgacatct tttggcaaga aagtgtgacg tctaaaaaac
     5701 gtttggcaga tgtgtctcaa gtggaagaat cggttgatcg agaagattat gtgcttcata
     5761 aattcttttt tgaagatgcc gttcaggatt ttgaagagct ttgtcaagaa ttgtatatca
     5821 gtcggacaac tttaaaacat gttttagcag gcattaagga gcgcataata ccttaccagt
     5881 tagagcttga aataactcac caacatattc aaataagagg taaagaagag gatattaggc
     5941 attttatcat ggactatttt tttgttacta gttttgataa taccttgtcc accatggtag
     6001 gtaatatctt tcttgaaggt attaattttg cagaaatcat gattattgtc ttggatgaat
     6061 gtcgggatgc taagttgaaa ctttctgatt ttgtcatgaa taatctagtc ttgcatattg
     6121 ccttaatggt tcagcgtatt cgatcggggt gtccattaga actgttttcc attccaatag
     6181 ctattcgtca gtctgatgag tatcaagtag ctctgcgcat tttgtatcga gtggaagaag
     6241 tgatggggat acgttttcct aaagaggagg ctaattatat tgcactgcat ctaaaggtca
     6301 aacattctgt tggtaagcat tggcaagatg ataataccga tgaaaagttg caggaccatt
     6361 taaaagcatg tattgccaag attagtcaat tgacaggcat gactttagaa acagatacca
     6421 atttatttca aggtttatta gcccacatga tgcccttgac cacacgtctt gaaaaccata
     6481 ttcagctgac taatccttta actgaggaaa ttaaaagcca gtatcctgaa atatttaccc
     6541 ttaccaagca gaccttttcg gatttgcttg tatgtcaaaa gaatgatgtc tcagatgatg
     6601 aatgggctta tattagtttg catttaatgg cggccattga gcgttattca aaccgtcata
     6661 aattgagggt tctagtagtt tgtgccacag gttatggtag tgccatgatg ttgaaaaatc
     6721 gcttggaaaa ggaatttgaa ggacgtttgc gtattgtcga tgttatcagt tactatgaaa
     6781 taaccgaaga gcgactgaaa accgttgatt tgattatttc ctctatcagc cttgccaatc
     6841 tgatgttttt aacaccagtg attactgtca gtgttttttt gtcaaatcag gatattgaaa
     6901 ctattcgaca atttattgga gaacaagaag gtatcaagaa agaggtcagt ttaccgtcac
     6961 agatgagttt agcaaaagca gagcaacttc taacaggtgt ttttagccct aatcgttttc
     7021 tgtatttaga cgagaaaatc tcaaaggaag acttgctttt acggatgatt gcttgtcttg
     7081 acgaggcggg aacagaaatg tttgtagagg acttctatca tcaaatggta ctgagggaaa
     7141 attacagccc agtcatctat ggagaagtgc ttgcctttcc gcatcctgct aatcccatga
     7201 cttattctga acaggtagta gtggctattt gccgagagcc tcttgagtgg gacaaggtgc
     7261 atcaagctgt tcattttgtc tttctattat ccccgtctaa agggcataat cataggttaa
     7321 aatatgtatc accaggtttg gcatcttttg tcaatcaggt ggagcttcag caaacgttac
     7381 tagaagaacc aaattattcc aaattcatga cagtgtttac cccacttatt catgattaaa
     7441 acgattaaaa ggagaaacct tatgaaatct attatgttag tttgtaatgc cggtatgtca
     7501 actagtatgt tggtgactaa gatgcaaaaa gcagcccaag cgcgtgacct tgaagtttct
     7561 atttgggcag ttcctgtgag tgaagctgac aacgaagtgg ctgctaatag cattgacgtt
     7621 ctcttgttag gtccacaagt caaattctta ctaaaggatt ttaaagataa gtttgaacca
     7681 gatattaaag tagatgccat taacatggct gattatggtc ttatgaacgg tgaaaaagtc
     7741 cttgaaacag ccctagcgat gatggaggag taggcatgac ggaaccatcc aacttagaag
     7801 ctattatggg cttaatcatg tatggaggcg aagccaaggg caaggccgta gaggctattc
     7861 atgcagcacg agatgggcag tttgaggagg ctcagcactt acttcaagaa gcgacaaact
     7921 cgttaaatat tgcccataaa tcgcagacac atttgctgtc ccaagaagca gctggtgatg
     7981 cggttgaatt gtctttattg atggtgcatg ggcaagatca catgatgact gccttagcct
     8041 ttattgattt agccaaggaa ttggttaccg tttacgacaa aatgtcagca atacagaggt
     8101 gtgagtcatg atcatttcag cagaaggaga tgtttccatt catcattgtt ttgaagccat
     8161 gctgatatcg ctccaagaag attatcgaca aaataccttg agacggttgt caagagatga
     8221 tatcaaagcg gggataaact atgttaaaca ttatggtaaa tacaaagagc gtacagtgag
     8281 ggtgcttatt acggccctag aagctccgca attatatact gcttgtttta gttctaatca
     8341 tgggataaag gaattggctt atcgattaga ggctctgggg gagaatcgga ctagaattac
     8401 ctattcttat gactatcatc ctctagatat ttttcaaaaa gcaaatcatt ttattgttgg
     8461 caaactgttc aaaaaaagcc tagaacgcca atctcaagct cagttagcag cccttatcca
     8521 atatgctaaa cagttgcagc aaccatcgtc tttgtgaaga ttattcacag accttggatg
     8581 agagagaagg gttcatttgg ataagagtcg attaccggtt atctctaagc atcacgttta
     8641 acaaggatga ttttgagata attgtgttag gcagacaccg ctgcttttaa ggttaccttt
     8701 gttacattaa aaaaataaaa agaggagaag atttatgtcg catttttctg ataagtttat
     8761 ggatatttca ggaaaaattg ggtcacaacg gcatttagta gctattcgtg attcctttat
     8821 ttcaatgatg cctattacca tggcaggttc agttgctgtc ttattgaacg tttttttacg
     8881 cgatattcca aacaatatgg gatggacagg gtttgcaaaa gctatgcaac ctgttatcga
     8941 catcaatggc tatgtttatt ttgggacaat tggtatcatg gcgctctttt ttgcctttgc
     9001 tttcggttac aatttagctg ttatgcataa gattaatcca ttggcaggag gattgatttc
     9061 ttttggctca tttattgcca ccctacctca gactttaacc atttcgacac cacttgaaaa
     9121 tgcctctgct aacctaatta gcaatttaaa agaaatgggg ctttcagtcg ttactgcggg
     9181 tggagcttca agtattgaaa ctagtcaatg gggagcaata gctctaaagt atgtcggtgc
     9241 cacaggcttg ttcacagctt taattattgg ctttttatca agttttgtgt atgcggcttt
     9301 gaccaaacgt aatattacta ttaccttacc agataatgta ccacctgcgg ttaataaagc
     9361 ctttgctgcc attattccag gaacagttgc gatttacgct tcggcaattt tcgcctacct
     9421 catcttcgca ttaacaggtt cttccctaag tgatgtcatt tcaacctata tccaattacc
     9481 attgctaggc ttatcacagg gtattggttc agttatcttg ttaaccttct tagttcaact
     9541 gctctggttc ttcggcttgc atggtcataa tgtcttagcc ccagtgatgg atggtattta
     9601 tatggtagct ttaacagaaa atacagctgc ctacaataca gcgcatagcg ctgctaattt
     9661 accatacctt tggacacgcg ggtcctttga tgcttatgct caaatgggtg gttcaggagt
     9721 aaccttggca ttgattattg ctatcttcat attctcaaaa cgagaagagc ataaaaccat
     9781 tgccaaattg tccgcaccaa tgggtgtttt caatatcaac gaaccaatta catttgggat
     9841 gccaattgtt ttgaatccaa cctttgtgat tccagggcta attgtgccac caatttgtgc
     9901 tagcatagct tattttgcta ctgcaatagg tttgattcca ccggtattct tatcagtacc
     9961 ttggattacc cctgttggac tttatgctta cctagctact ggaggtaata ttatggcagg
    10021 tttggtttca ctgtttaacc tctttgttgc cttccttatc tgggcaccat ttgtcatttt
    10081 ggccaataaa gaaaaagcga gtgaccttgc atgacagaaa aaaatggagc aactttttta
    10141 atggctgttg gcttttttct cattgccatt agtccgatgg ttggtaacaa atacttacac
    10201 atagtgacgg gactagctct tacgggatta ggttattacc tcactaaaca taaacgccca
    10261 taaaaaagca taggggacac tcttatgaag gagagagtca taagagtatc cctattttgc
    10321 tggtttgaat aataacagta accaaaaaag gcaaggaaac aggtttcctt gccttattat
    10381 tttttggaag ttagtatggt gtctgctcta ttagacgttt aagaccttgt ctaaaaattc
    10441 aatgagacga gggtgcttag gatgatcaaa aatctcttca ggagtaccat cctccaagaa
    10501 ttgaccaccg tctgtaaaga taacacggtt agctacttgg cgagcaaatc ccatctcgtg
    10561 agtaacaatt aacatggtca tgccttgttc tgccaagtct ttcataacgt tcaaaacgtc
    10621 cccaaccatt tcggggtcaa gagcagaagt tggttcatca aagagcataa tatctgggtt
    10681 catggctaaa cttctggcga tggcgacacg ctgtttttgc ccgccggata aacttcctgg
    10741 gaaggcgtcg gccttatcag aaagtccgac tttttctaga agtgccatgc cgtgcttttt
    10801 agcagcttct ttagactctt tacctaattc aacaggagca aagataatat tctcaagaac
    10861 agtcatgtga gggaaaaggt tgaagtgttg gaataccatc ccgatatttt cacgtgcctt
    10921 gtcaatatta gtcttaggat ctgataattc aaaaccgtcc accatgacct tcccactggt
    10981 aatcgtctct agaaggttga gggtacgtag aaaggttgat ttcccagaac cagaaggacc
    11041 aatgatacaa acgacatcac cttcgtagaa tttggcatca attcctttta agacttcatt
    11101 ttgaccataa gatttgtgta aaccttgaac atcaattttt aattctgtca ttagttaagc
    11161 ctcttttcta aacgttttgc aagtcttgtt aagagtgtaa tcatgataag gtaaataatt
    11221 gctaaaatag catacatgcg gaacgactgg taatttctag caataatgat tttacctgtt
    11281 tggaagagtt ccactaaacc aattgctgag acgattgttg tatccttcaa tgaaataaca
    11341 aactgattga taaagttagg taacattagt ttcacagctt gtgggagaat tacttttctc
    11401 atcgtggttc cgtaagacaa accaagactt cgactagctt ccatttgccc tgctggaaca
    11461 gcttcgatac caccgcgaac aatttcagca atataggctc cgccattaag tgacagtgca
    11521 attgtagcag ctaagaaatc attaatcggt gactggtggc cggtcatact ctcgataagg
    11581 tttggtactc cccagaaaat gaaggcagcc acaatcatca aaggaatccc tcgaacaacg
    11641 tccacaaaga ccgttgaaat gagtcgaagt gatttagttg gtgacacggc catcatccca
    11701 aagatgatcc cgataattat agcaatagca aatgaaataa gggttaaact gagcgtggtt
    11761 ccaagtcctg ccaatagttg tttgtagtta tttgataata ggcctgaaat agtagactca
    11821 tcagcacctt tttcagaagg agttgcagct ttcttagagt caaggtattt atctataatg
    11881 tcatcatact gaccagattt tttgagagca gctaagccat tgttgaacat ttcgattaat
    11941 tctggattgg ttcctttctt gacagcaaaa ccaacttcac cagtagaaat gccctcaaga
    12001 ggtgtttcaa agcgacgacc ttgagagata gcgtatttaa gaaccgcctc atcatccatg
    12061 atagcatcta cagaacctga atttaagcta tcatacatgc tagaaccatc atcaaatgcc
    12121 ttgacattat aaccatattt aggagcgttt tcttttaacc aagagtaaga tgaagtgccg
    12181 tttttagcac cgactgtttt tctgtctaag tcttcatagt tcttgatgtt ttttccagct
    12241 ttaacagcta aaatgatatt agaagtataa taaggatcag aaaaatcaaa gatagcttta
    12301 cgagcgtcag aaatagttgc accagcaatg accccatctg cttggctaga ttgcacagca
    12361 tttaaggcag catcgaaacc tggattagcg atttcaattt tgaaaccttg ttgtttagca
    12421 atagctttga ttaattctat gtcaatacca acgtatttgc ccttaccatt ttgaaattca
    12481 aaaggtgcaa aagacgaatc agagacaatt ttataactgt cctttatagg tgtagctttg
    12541 gcagatggat ttcccgttgc ctgactggtg gtagctttat catctgtgcc taaccacttg
    12601 gtcatgatag cttggtaggt accatcagct ttcatagctt taagagctgt attgaaatca
    12661 ttaactagat aatcatatcc gcttcccttt ttgacagcaa acccaaagtt tccaatgggc
    12721 tctcctttca tgttaatagc aatatcttgg ttttggctga ttgcgtattg gataaccgcc
    12781 tcatcatcca taacagcggc aatagaacca gcagataaac tattatacat aagatcacct
    12841 gtgtcaaatg ttttaacagt ataatcatac ttttttttat agttattcaa aaaggcttga
    12901 gccgctgttc catttttaac accgaccgtt tttcctttta agtcactgta ttttttgatg
    12961 gcattggctt tacgtgtcgc aatgacaatt ttggtatcgt aatatggctc tgagaaatga
    13021 aagactttct tacgagcatt cgtaatggtt gtaccggcca ttagagcact cgcttgacca
    13081 gattgaacag catttacagc tgcatcaaaa cccgggaaac tcatactgaa atcccaagat
    13141 tgacgtttgg ctacttcatt aataatatca acgtcaattc ctttgtaaat ttgatctgag
    13201 tctttaaatt caaatggggc ataagctgta tctgaaacaa tagcaatagt ttcagcactt
    13261 gcaatattgc atgtcaaaaa aatagacatt atcgcaagca gcaatacttt tattttgtgc
    13321 gtcat
//
LOCUS       DAUGFP010000044        12984 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.044, whole genome shotgun sequence.
ACCESSION   DAUGFP010000044 DAUGFP010000000
VERSION     DAUGFP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12984
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.044"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(184..947)
                     /locus_tag="VH663_001324"
                     /pseudo
     CDS             complement(184..947)
                     /locus_tag="VH663_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1112..1185
                     /locus_tag="VH663_001325"
     tRNA            1112..1185
                     /locus_tag="VH663_001325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1146..1148,aa:Arg,seq:tct)
     gene            complement(1342..1821)
                     /gene="rlmH"
                     /locus_tag="VH663_001326"
     CDS             complement(1342..1821)
                     /gene="rlmH"
                     /locus_tag="VH663_001326"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEQ8174522.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIDLTDERTP
                     DKASDAENQMIMAKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            2033..3256
                     /locus_tag="VH663_001327"
     CDS             2033..3256
                     /locus_tag="VH663_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEQ8174523.1"
                     /translation="MPSMKHILKSLSILLIGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVKISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKTVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            3315..4121
                     /locus_tag="VH663_001328"
     CDS             3315..4121
                     /locus_tag="VH663_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEQ8174524.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIITNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLK"
     gene            4354..5709
                     /gene="dnaA"
                     /locus_tag="VH663_001329"
     CDS             4354..5709
                     /gene="dnaA"
                     /locus_tag="VH663_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEQ8174525.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQAQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
     gene            5864..7000
                     /gene="dnaN"
                     /locus_tag="VH663_001330"
     CDS             5864..7000
                     /gene="dnaN"
                     /locus_tag="VH663_001330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEQ8174526.1"
                     /translation="MIQFSINRTLFIHALNATKRAISTKNAIPILSSIKIEVTSTGVT
                     LTGSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQH
                     QVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
                     ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDD
                     IETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAME
                     RAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYL
                     IESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN"
     gene            7075..7272
                     /locus_tag="VH663_001331"
     CDS             7075..7272
                     /locus_tag="VH663_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEQ8174527.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            7602..8717
                     /gene="ychF"
                     /locus_tag="VH663_001332"
     CDS             7602..8717
                     /gene="ychF"
                     /locus_tag="VH663_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEQ8174528.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            8787..9356
                     /locus_tag="VH663_001333"
     CDS             8787..9356
                     /locus_tag="VH663_001333"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEQ8174529.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            9359..12862
                     /gene="mfd"
                     /locus_tag="VH663_001334"
     CDS             9359..12862
                     /gene="mfd"
                     /locus_tag="VH663_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEQ8174530.1"
                     /translation="MNILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLRNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GISINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLVVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFTDNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYERLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEHEIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQISSLSKYVSADGKEPKINKLNDGRFQKTKQ
                     KVTRQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVAELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYISDERQK
                     IDIYKRIREIQSREDYLNLQDELIDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LMLFGERLSEIKIRKNNSVFK"
BASE COUNT         4389 a         2108 c         2352 g         4135 t
ORIGIN      
        1 ggatatctcc ttaacactct tactattata gcagattgat tttttttgtc tatacttttt
       61 ttaaaaaaaa tttgattttt tttaaaccgc aagttgtcaa gtcaagtgca acgagttcat
      121 agataactag agttccagag gttaagctgt tcgggatagc tcaaacagga attttgagga
      181 ttgctattga agaagtcgtc ttaggcgctc attgatggca gtaatatcat tgtcaagttc
      241 ttcagaggtt agtggattaa gtgagattcc tttagggaca tattctctga ggaggccatt
      301 gaagttctcg tttgttcctc tctcatgtga agaataggga tgtgcaaagt aaacgtcaac
      361 agcttctaag tctgagagta aactaaactc agaaccatta tccgctgtga tggatgcaat
      421 aggatactga ctgatgagat gcgtgacagc acggttaatg gtttgggact gcttgtcttc
      481 tagcttaact cctagtgcat agcgtgtctg gcgctctacc aaagtcaaca taacagcttc
      541 acctttggtt ttcttgccga gaaccaaatc aatctcccaa tgcccaaatt cagaacgatc
      601 attaatatat tctggacgtt cttcgataga tttacctaaa gtcttcttat tcgtcttcat
      661 gactttctta gaacgttttc tgatactaac catcttagac aaatcgattg gtttaataga
      721 ctatcagcga aataggcgtt gaagaaggtt tgttttccat tcttatcctt tacctgatct
      781 actgaaccac gttttatttc tcggcagatt gtagaacagt gacgccccag aagtcgagca
      841 atctcaacgg gtttctttcc cattttaaga taagcagcaa tttcaccccg ttctgtggct
      901 gaaagatgag aataggagga ttttctggta gaattaatat tagacatgtt catctgcttt
      961 ccatactgaa ttggggaatt ctagtatatc agacgaacat gtcttttttg ttgtacttca
     1021 ttttataacg cgggatataa tgttattttc tagttttttt aaaataaata ttgacagttt
     1081 tagataattt ttgttaaaat gatgtggtta aggtctcata gctcagctgg atagagcatt
     1141 cgccttctaa gcgaacggtc gcaggttcga atcctgctgg gatcaaagat aaaaaagcac
     1201 tgttttagtg ctttttttgt atttacctta gtgtattgaa aagataggat ttagtctgtc
     1261 tttttttaat atttgctttt aattatacca aactattatc taataaagtg gtaagagtta
     1321 gttattccag ttcacactct cttacttatg ataaggactg ccttgtgtaa tcataaaagc
     1381 acgataaatt tgctcagtaa ggactagacg cattaactga tgaggtaagg ttaacaatcc
     1441 aaaactcatt agcatattag ctcttttttt aataattgaa tctaatccaa gactcccacc
     1501 aataataaac gtgatagttg aatatccctt aacagtaacc cctgatatta attcactaaa
     1561 tgtctctgaa ggaaactgtt ttccttcaat agctaatgca attacaaaat cacgttcccc
     1621 aattttttta tggattcgct gagcttcctt agccatgatc atttggttct cagcatcact
     1681 tgctttatct ggtgttcttt catcagttaa atcaatcatt tcgaattgac aaaaacggga
     1741 aagtcttttt tgatattctg aaataccatc tttgaggtat ctttctttta gttttccaac
     1801 acaaattagt tttactttca tagcttaatt gtatcacaag aacattactt tttacacaat
     1861 ttatccacaa gttatttttc ttcaactact aataaaaacc tttgtacaaa ggtctttatt
     1921 agtaaaaaga aaagtaatcc acagcctgtg gattactttt ctttttttta tttttaaggt
     1981 ataattaagc aaagttctat aagctatgga caaataattc ggaggacaaa acatgccaag
     2041 tatgaaacat atcttaaaat ccttaagtat tttactaatt ggatttttag gaggattaat
     2101 agcaattatt acattcaata atctctaccc acattctcct tcaaaaataa attcaggtaa
     2161 ggcaacaact agtaatatgg tttttaacaa cacaaccaat actactaaag ctgtcaaagc
     2221 tgttcaaaat gcagttgtat cagttattaa ttatcaagat aacccttctt catccctttc
     2281 taacccttat acaaaactct ttggagaagg gcgttcaaaa gagaataagg atgctgaatt
     2341 atctattttt agtgaaggat ctggggtcat ttatcgaaaa gatggcaact ccgcttacgt
     2401 tgttactaat aaccatgtta tcgacggagc taaacggatt gaaattctta tggcagacgg
     2461 atctaaagtt gttggtgaat tagttggagc tgatacttat tcggatttag ctgttgttaa
     2521 gatctcttca gataagataa aaacagtagc tgaatttgca gactctacaa aactaaatgt
     2581 tggagaagtt gctattgcta tcggcagccc actaggaaca caatacgcta attctgttac
     2641 tcaaggaatc gtctctagtt taagtcgtac tgtaacttta aaaaacgaga atggtgagac
     2701 tgtctcaaca aatgctattc agacagatgc tgctattaac cctggaaact ctggtggacc
     2761 actaattaat attgaggggc aagtaatcgg aattaattca agtaaaattt cttctacccc
     2821 aactggtagc aacggtaata gtggtgctgt tgaaggaatt ggttttgcta tcccatctac
     2881 tgacgttatt aaaattatta aacaactaga aactaatggt gaagttatta gacctgctct
     2941 tggaatatcc atggtcaatt taaatgactt atccacaaat gctcttagcc aaattaatat
     3001 tccaactagt gtaactggtg gtattgtagt agcagaggtt aaggaaggaa tgccggcatc
     3061 tggcaaactt gctcagtacg atgtgattac agaaattgat ggcaaaacag ttaattcaat
     3121 tagtgattta caaagtagtc tatacggaca cgatattaat gatactatta aagtaacttt
     3181 ttatagagga actacaaaga aaaaagcaga tatcaaatta acaaaaacta ctcaagattt
     3241 gactaaaacg cagtaaacaa gctacctaaa tactattaca ttttatcaca taaaacagtc
     3301 caaaggactg ttttatgttt cttattctat ccaggaactc ccttatgaca aaagaattat
     3361 tgatagactt accaattgaa gatattatta caaatcccta tcagccaagg atacaattta
     3421 atcaaagaga actccaagac ctagcaactt ctattaaatc aaacggcctc attcaaccta
     3481 ttattgttcg aaaatctgac atttttggct atgagttagt tgctggagaa cgacgactta
     3541 aagcttcaaa aatggctggt ctaaaaaaag ttccagctat catcaagaag atctctacac
     3601 tcgagagcat gcaacaagct atagttgaaa atttacaacg ttctaacctt aacgctatcg
     3661 aagaagctaa agcctatcag ttattagttg aaaaaaaaca catgactcac gatgagattg
     3721 ctaaatatat gggaaaatca agaccttata ttagcaatac cttacgtctg ttacaactcc
     3781 cagcaccaat cattaaagca attgaagaag gaaaaattag tgcgggacac gcgcgtgctc
     3841 ttttaacttt gagtgacgat aagcaacaac tgtacctcac tcataaaata caaaatgaag
     3901 gcctaagtgt taggcaaatt gagcaactag ttacttctac tccaagttcg aagctatcta
     3961 aaaaaactaa aaatattttt gccacttctt tagagaaaca attggctaaa tcattgggac
     4021 tctctgtcaa tatgaagctg acagcaaacc atagtgggta ccttcagata tctttttcca
     4081 atgatgatga attaaacaga attatcaaca agctaaagta gcttgttgat attctgtttt
     4141 ttctttttta gttttccaca taaaaaatag ttgaaaacaa tagcggtgtc accttaaaat
     4201 ggcttttcca caggttgtgg agaacccaaa ttaacagtgt tattttattt tccacaggtt
     4261 gtggaaaaac taactattat ccattgttct gtggaaaact agaatagttt atggtagaat
     4321 agttctagaa ttatccacaa gaaggaacct agtatgactg aaaatgaaca aattttttgg
     4381 aacagggtct tggaattagc tcagagtcaa ttaaaacagg caacttatga attttttgtt
     4441 catgatgccc gtctattaaa ggtcgataag catattgcaa ctatttactt agatcaaatg
     4501 aaagaactct tttgggaaaa aaatcttaaa gatgttattc ttactgctgg ttttgaagtt
     4561 tataacgctc aaatttctgt tgattatgtt ttcgaagaag acctaatgat tgagcaaaat
     4621 cagaccaaaa tcaatcaaaa acctaagcag caagccttaa attctttgcc tactgttact
     4681 tcagatttaa actcgaaata tagttttgaa aactttattc aaggagatga aaatcgttgg
     4741 gctgttgctg cttcaatagc agtagctaat actcctggaa ctacctataa tcctttgttt
     4801 atttggggtg gccctgggct tgggaaaacc catttattaa atgctattgg taattctgta
     4861 ctattagaaa atccaaatgc tcgaattaaa tatatcacag ctgaaaactt tattaatgag
     4921 tttgttatcc atattcgcct tgataccatg gatgaattga aagaaaaatt tcgtaattta
     4981 gatttactcc ttattgatga tatccaatct ttagctaaaa aaacgctctc tggaacacaa
     5041 gaagagttct ttaatacttt taatgcactt cataataata acaaacaaat tgtcctaaca
     5101 agcgaccgta caccagatca tctcaatgat ttagaagatc gattagttac tcgttttaaa
     5161 tggggattaa cagtcaatat cacacctcct gattttgaaa cacgagtggc tattttgaca
     5221 aataaaattc aagaatataa ctttattttt cctcaagata ccattgagta tttggctggt
     5281 caatttgatt ctaatgtcag agatttagaa ggtgccttaa aagatattag tctggttgct
     5341 aatttcaaac aaattgacac gattactgtt gacattgctg ccgaagctat tcgcgccaga
     5401 aagcaagatg gacctaaaat gacagttatt cccatcgaag aaattcaagc gcaagttgga
     5461 aaattttacg gtgttaccgt caaagaaatt aaagctacta aacgaacaca aaatattgtt
     5521 ttagcaagac aagtagctat gtttttagca cgtgaaatga cagataacag tcttcctaaa
     5581 attggaaaag aatttggtgg cagagaccat tcaacagtac tccatgccta taataaaatc
     5641 aaaaacatga tcagccagga cgaaagcctt aggatcgaaa ttgaaaccat aaaaaacaaa
     5701 attaaataac atgtggaaaa gaatatcttt tatgaaatag ttatccacaa gttgtgaaca
     5761 accatttagt cttggattct ctcgtttatt tagagttatc cactatatac acaagaccta
     5821 ctactactac ttattattat acttattaaa taaaggagtt ctcatgattc aattttcaat
     5881 taatcgcaca ttatttattc atgctttaaa tgcaactaaa cgtgctatta gcactaaaaa
     5941 tgccattcct attctttcat caataaaaat tgaagtcact tctacaggag taactttaac
     6001 agggtctaac ggtcaaatat caattgaaaa cactattcct gtaagtaatg aaaatgctgg
     6061 tttgctaatt acctctccag gagctatttt attagaagct agttttttta ttaatattat
     6121 ttcaagtttg ccagatatta gtataaatgt taaagaaatt gaacaacacc aagttgtttt
     6181 aaccagtggt aaatcagaga ttaccttaaa aggaaaagat gttgaccagt atcctcgtct
     6241 acaagaagta tcaaccgaaa atcctttgat tttaaaaaca aaattattga agtctattat
     6301 tgctgaaaca gcttttgcag ccagtttaca agaaagtcgt cctattttaa caggagttca
     6361 tattgtatta agcaatcata aagattttaa agcagtagcg actgactctc atcgtatgag
     6421 ccaacgttta atcactttgg acaatacttc agcagatttt gatgtggtta ttccaagtaa
     6481 atctttgaga gaattttcag cagtatttac agatgatatt gagaccgttg aggtattttt
     6541 ctcaccaagc caaatcttgt tcagaagtga acacatttct ttttatacac gcctcttaga
     6601 aggaaattat cccgatacag accgtttatt aatgacagaa tttgagacgg aggttgtttt
     6661 caatacccaa tcccttcgcc acgctatgga acgtgccttc ttgatttcta atgctactca
     6721 aaatggtact gttaagcttg agattactca aaatcatatt tcagctcatg ttaactcacc
     6781 tgaggttggt aaggtaaacg aggatttgga tattgttagt cagtctggta gtgatttaac
     6841 tatcagcttc aatccaactt accttattga gtctttaaaa gctattaaaa gtgaaacagt
     6901 aaaaattcat ttcttatcac cagttcgacc attcacccta acaccaggcg atgaggaaga
     6961 aagttttatc caattaatta caccagtacg aacaaactaa gtaagaaaaa gctccctttt
     7021 aggagttttt ttgttattat aaatattaac gataatataa gtggagaaaa gggaatgtat
     7081 caaattggat catttgttga aatgaaaaaa cctcatgctt gtgtaattaa agaaactggt
     7141 aaaaaggcta atcaatggaa ggtactgaga gtaggagctg atattaaaat tcagtgtact
     7201 aactgtcagc atgtgattat gatgagtcgt tatgattttg agcgaaaatt aaaaaaagta
     7261 ctgcaaccat gaaagtcctt ttaaataaat aattctagct atatttgcaa ccaatacttt
     7321 cctaaaaaat tgctagtatg ccgttggaaa attcgctatt ctaatgttat cgaaagaaga
     7381 aagatggcca ttgacaatga cgaaagttgt agcacagtta cttagtttct cggcaaacta
     7441 tatcaaaatg agaataatac accaaacttt gataatttaa atgaaggtga aagagtggca
     7501 taatgataag caaatctgaa attttcttct tattcatacc gctttttctt ctattttttg
     7561 gtataataat cttgattgaa atttgaatgg agattcgcta aatggcttta acagcaggta
     7621 ttgtaggctt accaaatgtt ggtaaatcaa ccttatttaa cgcaattaca aaagcaggag
     7681 cagaagctgc taattatcct tttgcgacta ttgatcccaa tgttggtatg gtagaggttc
     7741 cagatgagcg tctgcaaaaa ttgacagaat tgattacacc taaaaaaaca gttccgacaa
     7801 cctttgaatt tactgatatt gcaggtatcg ttaaaggtgc ttctagaggg gaaggtctag
     7861 gaaataaatt tttagctaat attcgtgaag tcgatgctat tgttcatgtg gtacgtgctt
     7921 ttgatgatga aaatgtcatg cgtgaacaag gtcgtgagga tgctttcgtt gatcctatag
     7981 cagatattga cactattaat cttgaattaa tcttagctga tttagagtca atcaataaac
     8041 gttatgcgcg tgttgaaaaa atggcacgaa ctcaaaaaga taaagaatca gtagcagagt
     8101 ttaatgtttt gcaaaagatt aaacctgttt tggaagatgg gaaatcagct aggacaattg
     8161 agtttacaga agatgaagca aaagttgtta aaggtctctt tttattaaca actaaacctg
     8221 ttttatatgt agctaatgtc gatgaagata aagttgctaa tccagatggt attgattatg
     8281 tcaaacaaat tcgtgacttt gcagctactg aaaatgctga agtagttgtt atctcagcgc
     8341 gtgcagaaga agaaatttca gagcttgacg atgaggataa agaagaattt ttggaagcta
     8401 tcggtcttac tgaatcaggc gttgataaat taaccagagc agcttatcat ctcttaggcc
     8461 ttggaaccta ttttacagca ggtgaaaaag aggttcgtgc ttggacgttt aagcgtggta
     8521 taaaagctcc acaagctgct ggtattatcc attcagattt tgaaagaggt tttattcgtg
     8581 cagtaaccat gtcttatgat gatctaatga cctacggttc agaaaaagcc gtcaaagaag
     8641 ctggacgctt gcgtgaagaa ggaaaagaat atgttgtcca agatggggac atcatggaat
     8701 tcagatttaa tgtgtaatta tattaaaata atatcagaag gttggaagaa cattccagcc
     8761 cttttggcat tttagaaaga gaaaatatgg taaaaatgat tgttggtctg ggaaatccag
     8821 gctctaaata tgaaaaaaca aagcacaata ttggttttat ggctattgac aatattgtca
     8881 agaaccttga cgttaccttt acagatgata aaaattttaa agcacagata ggaagtactt
     8941 ttattaatca tgaaaaagtt tactttgtga aacctactac ttttatgaat aatagcggca
     9001 tagcagtaaa agcattacta acctactata atattgacat aacagattta attgttatct
     9061 atgatgattt agacatggaa gtcagtaaat tacgtttacg tagtaagggt tcagcaggag
     9121 gacataatgg cattaagtca atcattgccc acattggaac tcaggaattt aaccgaatca
     9181 aagttggtat tggacgacct ttaaaaggta tgactgttat taaccatgtg atgggccaat
     9241 tcaataccga agataatatt gctatttcgt taactcttga cagagttgtc aatgctgtca
     9301 agttttattt acaagaaaat gattttgaaa aaacaatgca gaaatttaat ggataatcat
     9361 gaatatttta gaattattta gtcagaataa gaaagtccaa tcctggcact ctggattaac
     9421 caccttagga agacaactgg taatggggtt atcgggttca agtaaagcat tggctatagc
     9481 ttccgcttat ttagatgatc aaaaaaaaat agttgtggtt acatcaactc aaaatgaggt
     9541 tgaaaaatta gccagcgatt tatctagttt acttgatgaa gaacttgttt tccaattttt
     9601 tgcagacgat gtggctgcag cggaatttat ctttgcgtca atggataaag ctctatcaag
     9661 aatagaaacc ctgcaatttt taaggaatcc taaatctcag ggcgttttaa ttgttagttt
     9721 atcaggctta agaactttat tgccaaaccc agatgttttt acaaagagtc agattcaact
     9781 aacagttgga gaagattatg atagtgatac tcttactaaa caactgatga caattggata
     9841 tcagaaggtc tcacaggtta ttagtccggg agaatttagc cgtcgagggg atattttaga
     9901 tatctatgag attacacaag aattgcctta tcgattggaa ttttttggtg atgatattga
     9961 tagtattagg caatttcatc cagaaactca aaaatctttt gaacaactag aaggtatttc
    10021 tattaatcca gcaagtgatc ttatttttga ggctagtgat tttcaacgtg gcattgagca
    10081 attagaaaag gctctacaaa cagcacaaga tgataaaaaa tcttatttag aagatgtatt
    10141 agttgtttca aaaaacggtt ttaaacataa ggatatccgt aaatttcaat cattatttta
    10201 tgaaaaagag tggtcattat tagattatat tcctaaggga acgccaatct tttttgatga
    10261 ttttcaaaaa ctagttgata aaaatgcaag atttgattta gagattgcta atctcttgac
    10321 agaagattta cagcaaggaa aggctctttc caatcttaac tactttacag ataattatcg
    10381 agagcttagg cactataagc cagcgacctt cttttcaaat tttcataagg gacttggaaa
    10441 tatcaaattt gatcagatgc atcagctaac tcagtatgcc atgcaggaat tttttaatca
    10501 atttcctttg ttgattgatg agattaaacg gtatcaaaaa aatcaaacaa cggttattgt
    10561 acaggtagag tctcagtatg cttacgaacg acttgaaaaa tcttttcaag attaccaatt
    10621 tcgtcttcct ttagtgagtg ctaatcaaat tgtttcacgt gaatcacaaa ttgtaattgg
    10681 agctatctcc agtggttttt attttgctga tgaaaagtta gcgttaatca cagagcatga
    10741 aatttatcat aaaaagatca aacgacgcgc tagacgatct aatattagca atgctgagcg
    10801 tttgaaagat tacaatgagt tagcagtagg tgattacgtg gttcataatg tacatggtat
    10861 cggtcgcttt cttggaattg aaacgattca gattcaggga atccatcgag attatgttac
    10921 tattcaatat caaaattcag accgtatttc tcttccaatt gaccaaatta gtagcttatc
    10981 aaagtacgtt tctgctgatg gaaaagaacc taaaattaat aaactcaatg atggtcgttt
    11041 ccaaaagaca aagcaaaagg tcactagaca agtagaggat attgctgatg accttctaaa
    11101 attatatgct gaaagaagtc agcaaaaagg attttcattt tcaccagatg atgacttgca
    11161 gcgcgctttt gatgatgatt ttgcttttgt agaaacagaa gatcaactta ggtctataaa
    11221 ggaaattaaa gctgatatgg agagcatgca gcctatggat cgtcttttag taggcgatgt
    11281 aggatttggt aagacagaag tagctatgag ggcagccttt aaagcggtga atgatcacaa
    11341 acaagtagct gtcttagttc caaccacagt cttggcccag cagcattatg aaaacttcaa
    11401 agcacgcttt gaaaattacc ctgttgaggt tgacgtctta agtcgtttcc gtagtaaaaa
    11461 agagcaagct gaaacactag aacgtgtacg aaaaggtcaa attgacatta ttatcggaac
    11521 ccatcgactc ctatcaaaag atgtggtctt ttctgattta ggattgattg tgattgatga
    11581 ggaacaacgg tttggtgtta agcataaaga aactttaaag gaattaaaaa ctaaggttga
    11641 cgtcttaacc ttaacagcta ctccgattcc tagaacctta cacatgtcta tgttaggtat
    11701 ccgagattta tcggttattg agaccccacc aaccaatcgt tatcctgttc aaacctatgt
    11761 tttggaaaat aatccaggtc tcgttagaga agctatcatt cgtgaaatgg atcgtggagg
    11821 acaaattttt tacgtttaca ataaagttga cactattgaa aagaaagttg cagagctaca
    11881 agaattagtc ccagaagctt ctataggttt tgttcatggg caaatgagtg aaattcaact
    11941 tgaaaatacc ttgattgact ttataaatgg tgattatgat gtccttgtgg caacaacaat
    12001 catcgaaaca ggagttgaca tttctaatgt aaacactttg tttattgaga atgctgatca
    12061 tatgggattg tcaactttat atcaactacg agggcgcgtc ggaagaagta atcgtattgc
    12121 ttatgcttac ctgatgtatc gtcctgataa ggtcctaaca gaagtctctg aaaagcgttt
    12181 agaggctatt aaaggcttta ctgaattagg ttcaggcttc aagattgcta tgcgagattt
    12241 gtctatacga ggagcaggta atattttagg ggcttctcag agtggcttta ttgattcggt
    12301 cggttttgaa atgtattctc agttattgga acaggctatt gctagcaagc aaggaaaaac
    12361 gactgttcgc caaaaaggta atactgaaat caatcttcag attgacgcct atttgccaga
    12421 tgattacata tcagatgagc gtcaaaaaat tgacatttac aagcgtattc gagaaattca
    12481 atcaagagaa gattatctca atttgcagga tgagctgata gatcgttttg gagagtatcc
    12541 tgatcaagtt gcctatttgt tagagatagc tctgctaaaa cattatatgg acaatgcctt
    12601 tgcggaatta gttgaacgca aaaataatca ggtcattgtt cgatttgaag taacttcttt
    12661 aacttatttc ttgactcagg attatttcga agccttatcg aaaacccatc ttaaagctaa
    12721 aattagtgaa catcagggga aaatcgatat cgtctttgac gttcgccatc aaaaagatta
    12781 tagaatttta gaagaattga tgttatttgg agaaaggctt agtgagataa aaatcagaaa
    12841 aaacaattca gtttttaaat aataatttga aacaactttt agactgaata gtattaatga
    12901 caatggaacg aactagctct ttatactttt gtcaaaaatc taagatcttt gctgcgaaat
    12961 taggatcttt tctaccattt aaaa
//
LOCUS       DAUGFP010000045        12141 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.045, whole genome shotgun sequence.
ACCESSION   DAUGFP010000045 DAUGFP010000000
VERSION     DAUGFP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.045"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(1..2274)
                     /locus_tag="VH663_001335"
     CDS             complement(1..2274)
                     /locus_tag="VH663_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ8174531.1"
                     /translation="MTDDKFDLLPLLDYIDPSKLSYQDWVNVGFALKHEGYTAMDWDI
                     WSQSDSRYKKGECFAKWDSFQGNGLGTVTGATITQLAKENGWTSDYRTSDEAHELDWD
                     STIDRDYKIIDKNWIEAKEIREPTNWSPVHDLITYINTLFESTDKVGYVTETYPIEID
                     KGIIYKPTKGAYDRTAGQLIQELQKNPNDIGAVLGDYKEEAGAWIRFNPLDGNGVKND
                     NVTDFRYALVESDSMELGKQYALFKELELPIATLVHSGKKSLHAIVKVDAKDYQEYRK
                     RVDYIYQICKKNGLDIDTQNRNPSRLSRMPGIMRNGHKQFLIDTNIGKTNYEEWYQWI
                     EDLNDDLPEPETLADEWDNLPELAPELIKGVLRQGHKMLIAGPSKAGKSFALIELSIA
                     LAEGQKWLGWQCEQGKVLYVNLELDRPSALHRFKDVYDAMGLPPANVANIDIWNLRGK
                     TVPMDKLAPKLIRRSLKKNYQAVIIDPIYKVLTGDENSADQMAHFTNQFDKVATELGC
                     SVIYCHHHSKGSQGGKKSMDRASGSGVFARDPDALIDLVELELTEDLIKARSEKAAAK
                     IYQQALQEKALGYYQQEVTLDDLESRYQMQQHFDKAIKDIMVKQPYLEAVKKAQHEVE
                     IATAWRVDGTLREFAKFQPVNMWFSYPKHEVDTTGVLADIQLEDSKPLWKKAKEARKS
                     KEENLKERNQKLETAYSALFDGSTPVTVQEIREYLDLKSNKSVENYIKEHDGYDVKKG
                     VVFQISDNKETEKKENN"
     gene            complement(2264..2893)
                     /locus_tag="VH663_001336"
     CDS             complement(2264..2893)
                     /locus_tag="VH663_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174532.1"
                     /translation="MGEYCHLKELDGLRFGSLTVINRNRNNSKGGNARWNCLCDCGNK
                     TVVIGSKLRSGYTKSCGCARKNDNAKGYSSTRLYRIWKGMMNRCYNHKNDNYKYYGGK
                     GISICDEWLTFINFRTWSLSNGYKESLTIDRINPKGNYTPLNCRWVSMKMQQNNKTNN
                     RYLSYLGQEYTIAEFSEKLNVTYWTVINQLKLGWSVERIVEEARMKNDR"
     gene            complement(2903..4486)
                     /locus_tag="VH663_001337"
     CDS             complement(2903..4486)
                     /locus_tag="VH663_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ8174533.1"
                     /translation="MQLRPYQEEARSAVQHEWEDGKKRTLLVLPTGCGKTIVFSKIIE
                     DRVRLGERVLVLAHRSELLEQASDKLMTATGLGTALEKAENTSLGSWFRVVVGSVQTM
                     QREKRLSKFPTNHFDTIIIDEAHHAISDGYQRVLQHFDSSNVLGVTATPDRGDKQNLG
                     KYFDSLAYEYSLVDAIKSGYLSKITAVTIPLTLDLSTVSQQAGDFKASEIGTALDPYL
                     EQIADEMVKQCANRKTVVFLPLVKTSQKFRDILNQKGFKAAEVNGESKDRAEVLEDFD
                     NDKYNVLCNSMLLTEGWDCPTVDCVVVLRPTKVRALYSQMVGRGTRLAPGKENLLLLD
                     FLWHTERHELCRPAHLIAKTPEVAQKMVDNMTEETEVTFELLEAEEVASKDVVAEREE
                     ALAKQLAEMRKRKRALVDPLQFEMSINAEDLVDYVPEFGWEMAPVSDKQKNALEKFGI
                     FTDDIGNAGKATKLLERLAKRRETGLTTPKQIRMLERYGFKNVGMWKFEVASNMINRI
                     AASGWRLPKGINAREYQPE"
     gene            complement(4486..5121)
                     /locus_tag="VH663_001338"
     CDS             complement(4486..5121)
                     /locus_tag="VH663_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174534.1"
                     /translation="MTEYNNNFDRELGWDDEITQDSVGFVQLVPGDYQFTVTNMERAR
                     HTPNPKSPGKLPACNKAVVTIQIETSEGIAQLTHNLFLHTTTEGMLSAFFGAIGQKKH
                     GEPLKMNWNTVIGAKGVARINKRKGTGDYADREYDNIKSMIYADEVDWTKVLNKDVAQ
                     PQQMTHQQSAQPTYQGQQAQGTNFAQQPATQSYQQGQMQTPQQPQGGWGGF"
     gene            complement(5143..5877)
                     /locus_tag="VH663_001339"
     CDS             complement(5143..5877)
                     /locus_tag="VH663_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174535.1"
                     /translation="MTENIKEALEYAVNTAHEKPKTILGSDGKEYYDRNKYSLVELKT
                     KYYPKTLNLNTLDSLIDYLKSDMNNINSKRLMVIVEGPREVIVCEEDDDDLNRNVLVT
                     VEAIIPNVQFGRYDSPSDFNISLQSLFVNADDRNTVIEFASALKIENGSEIVDDGIGQ
                     TATIKQGVASLAKAKAPNPVTLRPYRTFSEVEQPSSEFIFRINQLANMALFEADGGKW
                     RLDAINNIANYLKEELADQTNITILA"
     gene            complement(5879..6301)
                     /locus_tag="VH663_001340"
     CDS             complement(5879..6301)
                     /locus_tag="VH663_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174536.1"
                     /translation="MNKTIKLDLSAIGEGGLQEKVDKELEKVFDNILDPNTETKVARK
                     VTITLTMKPDDARQTVSTAMEVKSTLAPQTGVATTVLVGQKDGKVYANELKSAMPGQT
                     YFDDEAVLRTDIGEPIDHLEKGINEDVIDFNKQKKVGN"
     gene            complement(6341..7432)
                     /locus_tag="VH663_001341"
     CDS             complement(6341..7432)
                     /locus_tag="VH663_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ8174537.1"
                     /translation="MQITRGKRARAQRVIIYGPEGIGKSSFAANFPEPLFIDTEGSTD
                     NMDVARMDKPTSYTMLKNQIAWIKANPTCCKTLVIDTIDWAESLIVDDVCAQHGKKGI
                     EDFGWGNGYTYTKEEVGRFLNMLQELIELGINIVLTAHAQMRKFEQPDEMGAYDRWEL
                     KLGKKTSSQTAPLVKEWADMVLFANYKTVVMTSESKKKKATGGQRVLYTQHHPAWDAK
                     NRHGLPDEMPLDYAVIAHLFAQVPTQPVPQTPPVQETPASQTAHESVHEQAEKAPEQP
                     PMQPTSAPVAYPPSMPKALTDLMSAEQVTPDELVAVANIRGHFPPMTPIENFPSDYWN
                     MIVANWLATLEVIKTQVRTVEPPFTVEGA"
     gene            complement(7447..8766)
                     /locus_tag="VH663_001342"
     CDS             complement(7447..8766)
                     /locus_tag="VH663_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ8174538.1"
                     /translation="MSITINKLEIENVKRIKAVKIEPSATGLTIIGGNNNQGKTSVLD
                     AIAWALGGNKYKPSQAMREGSQVPPTLKITMSNGLIVERKGKNASLKVIDPNGQKGGQ
                     QLLDSFVEELAINLPKFMDSTPKEKADVLLEIIGVGDQLAELELKEKEIYNQRHAIGV
                     IADQKEKFAKEMTYYPDAPKQLVSISELIQQHQAILAKNGENAQKRQNVERIRYDYNQ
                     SILEVDRLRKLLADAEAKTNKLSEDLKIANTDAMDLHDESTAEIEANIADIDEVNRKV
                     RANFDKDKAEEDAKQQREQYNILTNDIESIRQQKRDLLINADLPLEGLSVDDGKLLYL
                     GQEWDNMSGSQQLIVATAIVRKLKPDCGFVLIDKLEQMDNITLEQFGKRLEQEGLQAI
                     ATRVSIGEECAIIIEDGYSVENKAHNFKTAEGGNFAETVAPTWKGEF"
     gene            complement(8766..8993)
                     /locus_tag="VH663_001343"
     CDS             complement(8766..8993)
                     /locus_tag="VH663_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174539.1"
                     /translation="MKQQLINDNEFLRDENRRLNNELAEHYFVVTAKANLLDVIIASD
                     FILKSTLDKCIAELDEIDQIALLKAWNKGEL"
     gene            complement(9117..9254)
                     /locus_tag="VH663_001344"
     CDS             complement(9117..9254)
                     /locus_tag="VH663_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174540.1"
                     /translation="MKNKKEQWTPKVHCFRKDGSQCEPENITVPISFAGYYQILLELG
                     V"
     gene            complement(9323..9553)
                     /locus_tag="VH663_001345"
     CDS             complement(9323..9553)
                     /locus_tag="VH663_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8174541.1"
                     /translation="MTINLKRLKAERIASGMTQCEVAQSMGWKTRTPYAKRENGIVSI
                     GADELAKITLIFGLPIEKIAIFFDKDVPVMER"
     gene            9737..10498
                     /locus_tag="VH663_001346"
     CDS             9737..10498
                     /locus_tag="VH663_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8174542.1"
                     /translation="MRTNSEIISLIQSKVEERKMSMSELARNVGIAKSTMSRYFNKTR
                     EFPLNRADDFAKALNISTEFLLGIDLNSEVDGSELLGIYRELEEQRRVIVLDTAKEQL
                     EEQTKIINFSDKKTDIEDIFEVKGTTFAAAASGFGRGFEADDYDTYTVYTDEEPPHYD
                     YAIGIRGDSMLPKYEQGDMLYIVDKGMSTYSGQLCIVVHNGNTYFKKVYTEHDGLRLV
                     SLNKKYSDIFIGYPPAEDTYIKIYDVVGSFTPIEF"
     gene            10525..10890
                     /locus_tag="VH663_001347"
     CDS             10525..10890
                     /locus_tag="VH663_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174543.1"
                     /translation="MTLKQLLKSKNLGEYFEAKNNAEKMNEIENRGLKQVMKNSVRQT
                     QLNSEIKQVIKKEKEAVRCPKCKSTNVGFMQQGKKTFSVKKAVAGTLLIGGTVMGFLG
                     EKGKKQWHCNECSCIFETK"
     gene            11014..>12141
                     /locus_tag="VH663_001348"
     CDS             11014..>12141
                     /locus_tag="VH663_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ8174544.1"
                     /translation="MRKVAIYSRVSTINQAEEGYSIQGQIEALTKYCEAMEWKIYKNY
                     SDAGFSGGKLERPAITELIEDGKNNKFDTILVYKLDRLSRNVKDTLYLVKDVFTANNI
                     HFVSLKENIDTSSAMGNLFLTLLSAIAEFEREQIKERMQFGVMNRAKSGKTTAWKTPP
                     YGYRYNKDEKTLSVNELEAANVRQMFDMIISGCSIMSITNYARDNFVGNTWTHVKVKR
                     ILENETYKGLVKYREQTFSGDHQAIIDEKTYNKAQIALAHRTDTKTNTRPFQGKYMLS
                     HIAKCGYCGAPLKVCTGRAKNDGTRRQTYVCVNKTESLARRSVNNYNNQKICNTGRYE
                     KKHIEKYVIDVLYKLQHDKEYLKKIKKDDNIIDITPLKKEIE"
BASE COUNT         3483 a         2410 c         2148 g         4100 t
ORIGIN      
        1 ctagttattt tctttctttt ccgtttcttt gttatcagaa atttggaaca caacaccttt
       61 tttgacatcg taaccatcgt gttccttgat ataattttca acgcttttat tggattttaa
      121 atccaagtat tcacgaattt cttgcactgt aaccggtgtt gaaccatcaa ataaggcgct
      181 ataagctgtc tcaagttttt ggttccgttc cttgagattc tcttctttac ttttcctagc
      241 ttctttagct tttttccaaa gaggtttact atcttccaac tgaatatcag ccaacacgcc
      301 agttgtatcc acctcgtgct tgggataact aaaccacata ttaactggct gaaacttcgc
      361 aaactcacgt agcgttccat caactcgcca cgctgtggca atttctacct catgctgggc
      421 ttttttgacg gcctcaagat atggctgttt aaccatgata tccttgatag ctttgtcaaa
      481 atgttgttgc atctgataac gactttctag gtcgtctagc gttacttcct gttgatagta
      541 tcctagagct ttttcctgca acgcttgttg gtaaatctta gcagctgctt tttctgagcg
      601 tgccttgata aggtcttcag tcaactcaag ctccactaag tcaatcaatg catcagggtc
      661 acgagcaaat actccagaac cacttgccct gtccatagat tttttgccac cttgacttcc
      721 tttagaatgg tggtggcaat aaattacgct acatccaagt tcagtggcca ccttatcaaa
      781 ttgatttgta aaatgggcca tctgatcagc actgttttca tcgccagtga gaactttata
      841 aatagggtcg ataataacag cttggtaatt tttcttcagt gagcgtctga taagtttagg
      901 tgctaattta tccattggta ctgtttttcc acggaggttc caaatgtcaa tgtttgccac
      961 attagcggga ggtaatccca tcgcatcata aacatcttta aagcggtgca aagctgatgg
     1021 tctgtctagc tccaaattga catagaggac tttcccttgt tcacattgcc aacctaacca
     1081 tttctgtccc tcagccaaag caatagataa ctcaatcaat gcaaatgact taccggcttt
     1141 tgatggtcct gcaattagca ttttatgtcc ttggcgtaac actcctttaa ttaattcagg
     1201 agccagctct ggcaaattat cccattcatc agctagcgtt tctggctctg gtaaatcatc
     1261 atttaaatct tcaatccatt gataccattc ttcataattg gttttaccaa tatttgtatc
     1321 aatcaaaaat tgcttgtgac catttcgcat aatacctggc attcgtgata gacgacttgg
     1381 attacgattc tgtgtgtcaa tatcaaggcc gtttttctta cagatttgat agatgtaatc
     1441 aacccgtttg cggtactcct gataatcttt agcatctact ttgacaatag cgtgtaatga
     1501 ttttttaccg ctatggacca atgtcgctat tggcaattct agttctttaa ataaagcata
     1561 ctgcttacca agttccatac tatcggattc aaccaaggca tacctaaagt ctgttacatt
     1621 gtcgtttttg acaccattgc catctaaagg gttaaatcgt atccatgcac cagcttcttc
     1681 tttatagtca ccgaggacag caccaatgtc attaggattt ttttgtaatt cttggataag
     1741 ctgaccagct gtcctgtcat aggctccttt tgttggttta tagataatgc ccttatctat
     1801 ttcaatcggg tatgtttctg ttacatatcc gactttgtca gttgattcaa aaagagtgtt
     1861 gatgtaagta atcaaatcgt gaacgggtga ccagttagta ggttctctaa tttcttttgc
     1921 ttcgatccag ttcttgtcaa taattttata gtcacgatca atagtgctat cccaatcaag
     1981 ctcgtgggct tcatcactcg tacggtagtc agatgtccac ccgttttctt tcgctaactg
     2041 tgtgatagtt gcgccagtga cagtcccaag tccattacct tggaaactat cccatttagc
     2101 gaaacattca ccctttttgt atctactgtc agactgtgac caaatgtccc aatccatagc
     2161 agtatatcct tcgtgtttta gggcaaaacc aacattcacc cagtcttgat atgatagttt
     2221 tgatggatca atataatcaa gtaaaggtaa taaatcaaat ttatcatctg tcatttttca
     2281 ttctcgcttc ttctactatt ctttctacac tccaacctaa tttcaattga tttattacag
     2341 tccaataggt aacatttaac ttctctgaaa attcagcaat agtatattct tgacctaaat
     2401 atgataaata cctattattc gttttattgt tttgctgcat tttcatactt acccatctgc
     2461 aatttagtgg agtatagtta ccttttggat ttattctatc aatggttaat gattctttat
     2521 atccattaga gagcgaccat gttctaaaat taataaaagt tagccattca tcacagattg
     2581 aaataccttt tccaccataa tatttgtaat tatcgttttt gtggttatag catctattca
     2641 tcatgccttt ccaaattctg taaagtcttg tagatgaata acctttagcg ttgtcatttt
     2701 tacgtgcaca tccacatgat tttgtataac cacttcttaa tttacttcca ataacaactg
     2761 ttttatttcc gcagtcgcaa agacaattcc atcgtgcatt gccaccctta gaattgttac
     2821 ggtttctatt aatgactgtc aatgatccaa atctaagtcc atctaattct tttaagtgac
     2881 aatattctcc catttttgtc tcctattcag gttggtattc tcgagcattg atgccttttg
     2941 gtaggcgcca tccgctagca gcaatgcggt taatcatgtt tgatgctact tcaaatttcc
     3001 acattccaac gtttttaaat ccgtatcgtt ctagcattct gatttgttta ggtgttgtta
     3061 ggccagtttc tctcctcttg gccaatcgct ccaataattt agtagcttta cctgcatttc
     3121 caatgtcgtc ggtaaaaata ccaaattttt ctaaagcgtt cttttgttta tcagaaacag
     3181 gagccatttc ccagccgaat tctggtacat agtccactaa gtcttcagca ttgatagaca
     3241 tttcaaattg caacggatca actaatgcac gtttacgctt acgcatttct gctaactgct
     3301 tagcgagtgc ttcttctcgt tctgcgacaa catctttgct agctacttct tcagcttcta
     3361 acaattcaaa ggttacttca gtttcctctg tcatgttatc gaccattttt tgagcaactt
     3421 ctggtgtctt tgcaattaga tgagcaggac gacaaagttc atggcgttcg gtatgccata
     3481 aaaaatctaa taatagtaga ttttctttcc ccggcgctaa tcgtgtgcca cgaccaacca
     3541 tttgactata aagagctctt acttttgtgg gtctcagtac aactacgcaa tcgactgtcg
     3601 ggcaatccca cccctccgtt aaaagcattg aattgcatag tacattatat ttatcattat
     3661 caaagtcttc taggacttca gcgcggtctt tcgattcccc attgacctct gctgctttaa
     3721 atcctttttg gtttagaata tcacgaaatt tttgggaagt tttaacaagc ggaagaaata
     3781 caactgtttt acggtttgcg cattgcttca ccatttcatc ggcaatttgt tctaggtacg
     3841 ggtcaagcgc cgtcccaatc tcacttgctt taaaatctcc agcttgctga ctaactgtag
     3901 ataagtccaa agtcaacggg atagtaactg cggtaatttt agataagtaa ccagacttga
     3961 tggcatctac taaggaatat tcataagcta aactatcgaa atacttacct agattttgtt
     4021 tgtcaccgcg atctggtgtc gctgtcactc ccaaaacatt gctactatca aagtgttgca
     4081 aaactctttg gtaaccatca gagatggcat gatgggcttc atcaataata attgtgtcaa
     4141 aatgatttgt aggaaactta ctcaaacgct tctctctctg catcgtttga actgatccaa
     4201 ctacaaccct gaaccatgac cctaaagaag tattctctgc tttttctaat gctgtcccta
     4261 atccagttgc tgtcatcaat ttatcactag cctgttctaa aagttcggac ctatgagcca
     4321 gaaccaaaac tctctctccg agccttacac ggtcttcgat aattttcgaa aagacaatag
     4381 ttttaccgca accagttgga aggacaagaa gagttctctt cttgccgtct tcccactcgt
     4441 gttgcactgc tgaccttgcc tcttcttggt aaggtctaag ctgcattaga atcctcccca
     4501 tccgccttga ggttgttgag gtgtttgcat ttgaccttgt tgatatgact gcgttgctgg
     4561 ttgttgtgca aaattagtgc cttgtgcctg ttgtccctga tatgtaggtt gagcagattg
     4621 ttgatgtgtc atttgctgtg gttgtgcgac atctttattt aatacttttg tccaatcaac
     4681 ttcatcagcg taaatcatag atttgatgtt atcgtattcg cgatcagcat aatcgccggt
     4741 gcctttacgt ttattaatac gagcaacccc cttagctccg atgacggtat tccagttcat
     4801 tttaagtggt tccccatgtt tcttttgacc aatagctcca aagaaagctg atagcatacc
     4861 ttctgttgta gtatgtagga ataagttatg agttaattgc gcgattcctt ctgatgtttc
     4921 tatttgaatc gtaacgaccg ccttgttaca cgctggtaat tttcctggac tcttcggatt
     4981 aggtgtgtga cgagcacgct ccatattggt tactgtgaat tgataatcac caggcactaa
     5041 ttgaacaaaa cctacactgt cttgtgtaat ttcatcatcc catcccaatt cacggtcaaa
     5101 gttgttattg tattcagtca tttttatttc tcctattttc tattaagcta aaattgtaat
     5161 gttagtttga tcagcaagtt cttcttttaa gtagttagca atattattga tagcatctaa
     5221 gcgccacttt ccaccatctg cttcaaacaa agccatatta gcaagttgat taatgcggaa
     5281 aatgaactca cttgaaggtt gttctacttc agaaaatgtg cggtatggac gtaatgtaac
     5341 tgggtttggc gccttagctt ttgcaaggct tgccacacct tgtttaattg tggcagtttg
     5401 accaatgcca tcatcaacaa tttctgagcc attttcgatt tttaatgcac tagcgaattc
     5461 aataactgtg ttacgatcgt cagcgttaac gaataatgat tgcagactaa tattaaaatc
     5521 agaaggtgaa tcatagcggc caaattgaac atttggaata attgcctcca cagttactaa
     5581 cacatttcga ttaaggtcgt catcatcttc ttcgcaaaca ataacttcac gagggccttc
     5641 tacaatgacc ataagacgct tggaattaat attgttcata tctgatttaa gataatcaat
     5701 taaactgtca agcgtgttta aattcaatgt ttttggataa tacttagttt taagttcaac
     5761 caaagaatat ttgttgcgat cataatactc tttaccatca ctacccaaga tagtcttagg
     5821 tttttcgtga gctgtattta ctgcatattc caaagcttct ttaatgtttt ctgtcatgtt
     5881 agtttcctac tttcttttgc ttattaaaat caataacatc ttcattaata cctttttcaa
     5941 ggtgatcaat tggttcaccg atgtcagttc ttagcactgc ctcatcgtca aaataagttt
     6001 gaccaggcat cgcgcttttg agctcgttag cataaacctt accatctttt tgaccaacaa
     6061 ggaccgttgt agccacgcct gtctgtggtg ctagcgttga cttaacttcc atagctgttg
     6121 aaacagtttg acgggcatcg tctggtttca tagttaacgt aatagttact ttacgggcta
     6181 cttttgtttc tgtattagga tctaagatat tgtcaaagac tttttccaat tccttgtcta
     6241 ctttttcttg taagccacct tctccgatgg cagacaagtc taatttgatt gttttattca
     6301 ttgtttgtta tacctcgcta taatttctaa ttcccaaaac ctatgcgcct tccacagtaa
     6361 agggtggctc gacagtacgc acttgcgttt taatcacttc taacgtagct aaccaattgg
     6421 ccacaatcat attccaataa tctgacggga agttttcaat tggtgtcatt ggcgggaagt
     6481 gtcctcggat gtttgctact gccacaagct catcaggagt gacctgctct gccgacatca
     6541 agtcagtcaa tgcttttggc atacttggtg ggtaggctac cggcgcagat gtcggttgca
     6601 taggtggttg ttctggtgct ttttcagctt gctcatgtac ggattcatgt gcggtttgtg
     6661 atgcaggagt ttcctgtact ggtggcgttt gtggcactgg ttgagttggt acttgtgcga
     6721 atagatgtgc gattactgca tagtcaagag gcatttcgtc tggtaaccca tgacggtttt
     6781 ttgcatccca cgctgggtga tgttgcgtgt atagcacacg ttgaccacca gttgcctttt
     6841 tcttttttga ttcgctagtc ataactactg ttttgtagtt ggcaaacaag accatatcag
     6901 cccattcttt gaccaagggg gcagtctgtg agcttgtctt tttaccaagt ttcaactccc
     6961 aacggtcata agctcccatc tcgtcaggtt gttcaaattt tctcatttgt gcatgggcag
     7021 ttaatacaat attgataccg agttcaatca gttcttggag catattcaag aatcgaccta
     7081 cttcctcttt tgtgtaggta taaccattgc cccaaccaaa gtcctcgata cctttcttgc
     7141 catgttgagc acagacatca tctactatta agctttctgc ccaatcgatt gtgtcaatga
     7201 ccagtgtctt gcaacatgtt ggattcgctt taatccatgc aatttgattt tttagcatag
     7261 tgtagcttgt tggtttatcc atacgggcca catccatatt gtctgttgat ccctccgtat
     7321 caatgaacaa tggttcagga aaattagcag caaaactaga tttaccgatt ccttctggtc
     7381 cataaatgat gacacgttga gcacgtgctc gtttacctct tgtaatttgc atatttacct
     7441 ctctttctaa aactcacctt tccaagtcgg tgctactgtt tcagcaaaat taccaccctc
     7501 tgctgtttta aagttatgag ctttgttttc cactgagtat ccatcttcta taatgatggc
     7561 acactcttca ccaattgata cacgggttgc aatagcttgc aaaccctcct gctctagccg
     7621 tttaccaaat tgttcaagtg tgatattgtc catctgttcg agtttatcaa tcaaaacaaa
     7681 gccacaatct ggttttagtt tacgtacaat agcagttgct actattagtt gttggctgcc
     7741 tgacatgtta tcccactctt gaccaaggta aagtaacttt ccatcatcta ctgataagcc
     7801 ttcaagcggc aaatctgcat taatcaataa gtctcttttt tgttgacgaa ttgactcaat
     7861 atcattagtc aatatgttat attgttcacg ctgctgttta gcatcttctt cagctttatc
     7921 tttatcaaag ttagcgcgca ctttgcggtt gacttcatca atatcggcaa tattcgcctc
     7981 gatttcagct gttgattcat catgtaaatc catagcgtca gtatttgcta tttttaaatc
     8041 ctcgcttaac ttattcgtct tagcttctgc atctgctaat aacttgcgta aacggtcaac
     8101 ttccaagata gattgattat aatcatagcg aatacgttct acattttgac gcttttgagc
     8161 attctctccg tttttggcta agatagcttg gtgttgttgg ataagctcag aaatacttac
     8221 cagttgttta ggtgcgtcag gataatatgt catctcctta gcaaactttt ctttttggtc
     8281 tgctataaca ccgatagcgt gacgttgatt gtatatttct ttttctttta gctctaactc
     8341 tgctagctga tcaccgacac caataatttc aagcaagaca tctgctttct cttttggtgt
     8401 actatccata aatttcggca agttaatagc caattcttct acaaagctat ctagcaactg
     8461 ctgaccgcct ttttggccgt ttggatcaat tactttaaga ctggcatttt tgcctttccg
     8521 ttcaacaata agtccatttg acatggtaat tttaagcgta ggtggcactt gcgatccttc
     8581 gcgcatagct tgactaggct tgtatttatt accgcctagt gcccaagcaa tagcatctaa
     8641 tacgcttgtt ttgccttggt tattattacc accgataatg gtcaagcctg ttgcagatgg
     8701 ttcaatcttt accgctttaa tacgcttaac gttttcgatt tcaagtttgt ttattgttat
     8761 tgacattata attctccctt gttccatgct tttaacaggg ctatttgatc aatttcatcc
     8821 aattcagcaa tgcatttatc tagcgtactt ttcaagataa aatcactagc aataatgaca
     8881 tctaataaat ttgctttagc tgtaactaca aagtaatgtt ccgccaattc attatttaac
     8941 ctgcgatttt cgtctcgcaa aaattcgttg tcgttaatta gttgttgttt catacttttt
     9001 ttctcatata agcgtggaat gcatcatact gcttttcaga tgacaatcgc ttagtatcgt
     9061 gtttaatcgg ttcctgtttt tttggttttg taaaaataaa atctaatagt ttcatgttat
     9121 actcctagtt ctaataaaat ttgatagtaa cctgcaaaag aaattggcac tgttatattt
     9181 tctggctcac attgtgaacc atcttttcga aaacagtgaa cttttggggt ccattgctct
     9241 tttttgtttt tcatggtaaa attgcctcat aagaatctgt ctgtcccgta aactgctatt
     9301 ttacagggct tttttatttg catcatcgtt ccattacggg aacgtcttta tcaaaaaaaa
     9361 ttgctatttt ttcgattggt aatccaaaaa tcaaagtaat ttttgcaagc tcatcagctc
     9421 caattgaaac aataccgttc tctctttttg catatggtgt tcgtgttttc caccccatag
     9481 attgtgcaac ttcacattgt gtcataccgc tagcaattcg ctcggctttt agtcgcttca
     9541 aattgattgt catatacata ctcctttttt tatttttgtt cccgttttgg aacaccttta
     9601 ttataaaacc tacgttccaa attgtcaacg attttttcta aaaaaaatac gtttatttgt
     9661 tttttagcgt tttattgtat ttttttggga acgatggtat aatgatatta ctaaataaca
     9721 ttaaggataa caaaacatga gaacaaacag cgaaattatt tctttgatac agagcaaagt
     9781 agaagaaaga aagatgtcaa tgagtgagtt ggctagaaat gttggtattg caaaatcaac
     9841 gatgtctaga tattttaata aaacaagaga atttccttta aacagggcag atgattttgc
     9901 taaagcactt aatatatcaa cagagttttt acttggtatt gatttaaata gcgaagtaga
     9961 tggttcggaa ctgcttggaa tataccgcga attagaagaa caacgaagag taattgtttt
    10021 agatacagca aaagagcaat tggaagaaca aacaaaaatc atcaatttca gtgataaaaa
    10081 aactgatatc gaagatattt ttgaagttaa aggaacgact tttgctgcag cagcaagcgg
    10141 atttggtaga ggttttgaag cagatgacta tgatacttat acagtttata cagacgaaga
    10201 acctccacat tatgattatg ctattggcat aagaggcgat agcatgttac caaagtacga
    10261 gcaaggagat atgctttata tcgttgataa aggaatgtca acatacagcg gacaattatg
    10321 tattgttgta cataacggga acacttattt taaaaaagtt tacactgaac atgatggact
    10381 tcgattagta tcacttaata aaaaatatag cgatattttt attggctatc caccagcaga
    10441 agatacatac attaaaatat atgacgttgt tggtagtttt acaccgattg aattttaact
    10501 aatgttttta gagaggacaa tgatatgacg ttaaaacaac ttttaaaatc aaaaaatctg
    10561 ggagaatatt ttgaagcaaa aaacaatgca gaaaagatga atgagattga aaaccgtggc
    10621 ttaaagcaag taatgaaaaa ctcagtacga cagacccaat taaatagcga aataaaacaa
    10681 gtgataaaaa aagaaaaaga ggcggtaaga tgtccaaaat gtaaaagtac taatgttgga
    10741 tttatgcagc aaggtaaaaa gacattttct gttaaaaagg ccgtcgcagg aactctttta
    10801 ataggtggaa cagttatggg atttttagga gaaaaaggga agaagcaatg gcactgtaac
    10861 gagtgcagct gtatatttga gacaaaataa aaaaagcccc acgctctcaa agtttggcga
    10921 ctctgagcgt gaggcgaatc tagtatagta aaaacctgct ttaagtaggt ctctttactg
    10981 tactcatttt aacaaaaaat gaggtaaaaa acaatgagaa aagtagctat ttactctaga
    11041 gtatcaacaa taaatcaagc cgaagaagga tattccattc aaggacagat tgaagcttta
    11101 acaaagtatt gtgaggcaat ggaatggaaa atttacaaaa actactctga tgcaggtttt
    11161 tcaggcggta aacttgaacg accggcaata acagaattga ttgaagacgg taaaaacaat
    11221 aaatttgata ctattttagt ctataaacta gacaggctat caagaaatgt aaaagacaca
    11281 ctttacttag tcaaagatgt atttactgct aacaatatcc attttgtcag cttaaaagaa
    11341 aatatcgaca cttcttcagc aatgggaaat ctctttctca ctctcttgtc agctattgca
    11401 gagtttgaaa gagaacagat taaagagcga atgcagtttg gtgttatgaa tagagcaaaa
    11461 tctggaaaaa caacggcttg gaaaacacca ccttatggat atagatacaa caaagacgaa
    11521 aaaacattgt cggtcaacga gttagaggct gctaatgtca ggcagatgtt tgacatgata
    11581 atctctggct gctcaatcat gtcaattaca aattacgcga gagacaattt cgtcgggaat
    11641 acgtggacac atgtaaaagt aaaaagaata ttagaaaacg aaacatataa aggattagtg
    11701 aagtataggg aacaaacttt ctcaggtgat catcaagcca ttatcgacga aaaaacatat
    11761 aataaagcgc aaatagcttt agcacatagg acagatacga aaacaaatac tagacctttt
    11821 caagggaaat atatgctttc tcacatagca aaatgcggtt attgtggtgc ccctttaaaa
    11881 gtatgcacag gaagagctaa gaacgatggt acgagaaggc aaacttacgt ttgtgttaat
    11941 aaaacagaaa gtttggctag acgaagtgtt aataattata ataaccaaaa gatttgtaac
    12001 actggtcgat atgaaaaaaa acacatcgaa aaatatgtta ttgatgttct ttataaactt
    12061 caacacgata aagaatatct taaaaaaata aaaaaagatg acaatataat tgatattaca
    12121 cctttaaaaa aagaaataga g
//
LOCUS       DAUGFP010000046        12041 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.046, whole genome shotgun sequence.
ACCESSION   DAUGFP010000046 DAUGFP010000000
VERSION     DAUGFP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12041
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.046"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            152..370
                     /locus_tag="VH663_001349"
     CDS             152..370
                     /locus_tag="VH663_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174545.1"
                     /translation="MEIYKQRMIEEYKQLKKRAEKLSIVLNRYYLDELDFELSCPIEL
                     LQTQWHIMGAYLKILEQRFLVEGIYFND"
     gene            363..758
                     /locus_tag="VH663_001350"
     CDS             363..758
                     /locus_tag="VH663_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEQ8174546.1"
                     /translation="MIEFFLPMDKIPTTTHQQKKVTVINGKPHFYEPESLKNARDKFT
                     SLLAQHVPPSKLDGPIRLTVKWLFPKIKGSTNGQYKTTKPDTDNLQKLLKDCMTELGF
                     WNDDAQVASEIIEKFWADTVGIYVKVEEL"
     gene            755..976
                     /locus_tag="VH663_001351"
     CDS             755..976
                     /locus_tag="VH663_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174547.1"
                     /translation="MKIDYINFFEQVVPDWMRESNVKMQEVGFNTEAYWQWANQSIVA
                     ICEKYGNDSLINGQFHLIWEWLEDKANGG"
     gene            979..1251
                     /locus_tag="VH663_001352"
     CDS             979..1251
                     /locus_tag="VH663_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174548.1"
                     /translation="MTEEQELIKPLLEEIERKDDRLYMQSNVIAVLMTAVLMLLMISI
                     ALQNHYEPQITGLRTQLSRTQKQLKRASEDRTRQTKRIAELTGNGG"
     gene            1254..1589
                     /locus_tag="VH663_001353"
     CDS             1254..1589
                     /locus_tag="VH663_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174549.1"
                     /translation="MDYLMIGIIVLSVVIFIMGFFILYYQAIIVNSMKNDFRRMRKEL
                     REELGFDSYNWSDSFRNMRRDIKSNKELISEINSLQIIKKAKEAKRLEELEQTKANVE
                     KEINKLNGG"
     gene            1780..2181
                     /locus_tag="VH663_001354"
     CDS             1780..2181
                     /locus_tag="VH663_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174550.1"
                     /translation="MKNENFLGYDLAELLEEKLVGKRLKSIDDEKIVLADGTVIKIEL
                     NEGCGGCGNGWSELNITTENPNLESAVMGVEYTEKYSEWDDEFKIFVYMTDNSVIEIY
                     GYDGVGNGYYGYGFWVTVKNPGDLIESELED"
     gene            2181..2816
                     /locus_tag="VH663_001355"
     CDS             2181..2816
                     /locus_tag="VH663_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEQ8174551.1"
                     /translation="MLTTNQIHKLLGVKEVYKAPDTLIKIILDKEKREDLFRQFLKYE
                     TDVSYDWFMQYFEEEQADRKNKKQDFTPKSVSTLLSKIISGNQYYEVAVGTGGILIQA
                     WQEQRLNDSPFTYRPSKYWYHVEELSDKAVPFLLFNMSIRGINGVVVHGDSLTRQVKN
                     IYFLQNTKDDMLSFSDINVMPRTQDIEREFNVKEWIGDGIEHIENPLIEWI"
     gene            3081..3518
                     /locus_tag="VH663_001356"
     CDS             3081..3518
                     /locus_tag="VH663_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEQ8174552.1"
                     /translation="MGNIPTTKANNFLEELKTIPHLIETLERDANLMSRSLVKSPQWS
                     DMRVSGGVKQSQEDKNIKMLHMVSYYSDQIERLKDRRQEMANLIVQSMGICESHVLLT
                     TYLDCDGDYERARERLNIGNRNKYFMFVRRGKESLELILKNTN"
     gene            3680..6040
                     /locus_tag="VH663_001357"
     CDS             3680..6040
                     /locus_tag="VH663_001357"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ8174553.1"
                     /translation="MEFVDKKLSEITPYKNNPRNNDEAVGPVAESIKEFGFKVPIVVD
                     KNGEIVNGHTRYKAAQKLGLETVPVIVADDLSEEQIKAFRLADNKVGEIADAYEVKAT
                     RERNGNYSLYIKYPLDGVFASVFKEEMKIKSDAGRRTKWQTFEINRVLRNSKDHIEIF
                     ARHISMRTQDIALKPFVNGASVGAESALEIWKKNLVGDDKFDVKSDILTLGSFNWEID
                     KIGNARGALGGVAGSILDVYGGEYEFDNRTIILRKQMGRKAPTVLEYGRNIVSVEEER
                     LLDGNYTSIYPYVRYTPQPKPQEEPPGKPHVGEHKQPEEQLVTLPEFILDGQYLSLYA
                     QRRIQMVDLSSHFNDDKNKKEPTIEEIRKLAQKYLKDNNVGAPKVSIEVDYIDLSQTL
                     DYQDFRVMEEVELCDIVPLYYPKFGITTESEKVVEIVYDVYTDSNHTIKLGTIGQSIS
                     KSLTGGVSERINALENNQKVITNSQKQFELNLPKYLNDLNGKRVWYEKPDDNIEHKIG
                     DYWFEKNGKYQRTWIWDGNQWVKVLDTEDLNPNQRAFDAAIAELEKAKKAQEEINQRT
                     DKELEEFRATLKNLALPEEAIKKITEAIKVDDIPSIKQSFDDLKNRVSETSEESRLTA
                     EILGNNGKTRYNKNLLVGEPNRTKSYDQDYIEVEANDGGFKRGETYTISFSQTCEPLK
                     KVAVTLTQANNKGVKLVLTPTKAKMEAQTFEVAKDKQSIEVYPLSYTAVLTGDWYKSK
                     QIDLNASEVRELALEMDYKEIADAKGATITGAWSDSPQIILDGGKK"
     gene            6037..7059
                     /gene="hylP"
                     /locus_tag="VH663_001358"
     CDS             6037..7059
                     /gene="hylP"
                     /locus_tag="VH663_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase HylP"
                     /protein_id="HEQ8174554.1"
                     /translation="MSENIPLRVQFKRMTASEWARSDVILLESEIGFETDTGFARAGD
                     GHNRFSDLGYISPLDYNLLTNKPNIDGLATKVETAQKLQQKADKETVYTKAESKQELD
                     KKLNLKGGVMTGQLKFKPAATVAYSSSTGGAVNIDLSSSRGAGVVVYSNNDTSDGPLM
                     SLRTGKETFNKSALFVDYSGKTNAVNIAMRQPSTPNFSSALNITSGNENGSAMQLRGS
                     ERALGTLKITHENPSLEANYDKDAAALSIDIVKKQKGGKGTAAQGIYINSTSGTTGKL
                     LRIRNLNDDKFYVKPDGGFYAKETSQIDGNLKLKDPIANDHAATKAYVDGEVEKLKAL
                     LTAKQM"
     gene            7072..8958
                     /locus_tag="VH663_001359"
     CDS             7072..8958
                     /locus_tag="VH663_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEQ8174555.1"
                     /translation="MSRDPTLTLDESNLVIGKDGRVHYTFTASQDNQTVRLASNCLGT
                     AHFNQLMIERGDKATGYVAPVVVEGTGNPTGLFKDLKEISLELTDTANSQLWSKIKLT
                     NRGMLQEYYDGKIKTEIVNSARGVATRISDDTDKKLALINDTIDGIRREYRDADRKLS
                     ASYQAGIEGLKATMANDKIGLQAEIKASAQGLSQKYDDELRKLSAKITTTSSGTTEAY
                     ESKLAGLRAEFTRSNQGTRTELESQISGLRAVQQTTASQISQEIRNREGAVSRVQQDL
                     ASYQRRLQDAEDNYSSLTHTIRGLQSEVGSPTGKIQSRLTQLAGQIEQRVTRDGVMSI
                     ISGAGDSIKLAIQKAGGINAKMSGNEIISAINLNSYGVTIAGKHIALDGNTTVNGTFT
                     TKIAEAIKIRADQIIAGTIDAARIRVINLNASSIVGLDANFIKAKIGYAITDLLEGKV
                     IKARNGAMLIDLNSAKLDFNSNATINFNSRDNALVRKDGTHTAFVHFSNATPKGYTGS
                     ALYASIGITSSGDGVNSASSGRFAGMRCFRHATGYNHTAAVDQTEIYGDSVLIADDFN
                     INRGFKFRPDKTKKILDMNNLYDAVVALGRCWGHLRNAGWDTARSNFIAAVTRELSNY
                     IDII"
     gene            8970..9398
                     /locus_tag="VH663_001360"
     CDS             8970..9398
                     /locus_tag="VH663_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEQ8174556.1"
                     /translation="MDLTLKNKDLNTFYSVLDKIKITNMRANRGRAKLLAKVVDKINE
                     YAKDEADIIDLYALKNKDGKFVIDEHKNIKIEDPKKIDELNDLLTELGNEDITIKGHE
                     YSKRFIDFLEYLAESEDEFTSEEIVLIDNILEQFEESKGE"
     gene            9401..10018
                     /locus_tag="VH663_001361"
     CDS             9401..10018
                     /locus_tag="VH663_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEQ8174557.1"
                     /translation="MRNWKVTGKYPQYDSTGAVASTHIIITAEDGSVISQPIKQDLTS
                     TNDTEIIKAALEEFKKSEYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQRMINLQTIHVLTSGGKVEPDIYKGMLELIEPAKKGEYQAYDVFTVVDSTKEED
                     GEAGEGNLVFVHVNEPFTYEAQTLEELESEDKVTVIKYAELVKQD"
     gene            10029..10328
                     /locus_tag="VH663_001362"
     CDS             10029..10328
                     /locus_tag="VH663_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174558.1"
                     /translation="MHFDLLQIGAACTALLSILGVWGFIVNPFKKAMEANEFAMAQLK
                     DSIKELAYELKNLDRDREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            10325..10510
                     /locus_tag="VH663_001363"
     CDS             10325..10510
                     /locus_tag="VH663_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174559.1"
                     /translation="MNKWFKKVAIKTIKTMAQTAVGLIGSSVLITDINWPTMLSAVLL
                     SGLTCVLMNVSQIKEEE"
     gene            10623..11825
                     /locus_tag="VH663_001364"
     CDS             10623..11825
                     /locus_tag="VH663_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEQ8174560.1"
                     /translation="MAFLDKIKSAVIAEWHTHKILPSLTAAQAILESGWGKHAPHNAL
                     FGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRAYDSWDESIADHGQFLVDNPRYE
                     AVIGETDYKKACYAIKAAGYATASSYVELLIQLIEENDLQSWDREALKNNKEETMTTA
                     NEIVQYCVNLANSGMGVDKDGAHGTQCCDLPCFVAKNWFGVDLWGNAIDLLDSASAQG
                     WEVHRMPTEANPKAGATFVQSVPYHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDALYV
                     GAPARFNTRDFTGVIGWFYPPYQGDTVTQPVSTEPQTSDTIVETAKTGTFTLDVAEIN
                     IRRWPSLASEVVGIYKQGDTVSFDSEGYANGYYWISYVGGSGMRNYLGIGQTDKDGNR
                     ISLWGKLN"
     gene            complement(11893..>12041)
                     /locus_tag="VH663_001365"
     CDS             complement(11893..>12041)
                     /locus_tag="VH663_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ8174561.1"
                     /translation="GTKDGKHEQIDLFDSPNEGTRSDIFAKYKDNRIINMKNFSHFDI
                     YLEK"
BASE COUNT         4043 a         2157 c         2747 g         3094 t
ORIGIN      
        1 ataactcttt cagagttatt ataaaatgtt tttccttcgt caaaagtcaa agggaaaagg
       61 aagaggggcg actgagctac gccccctctg tcctttgtcc catatttgac aaaggcgcat
      121 ataaaaaaca caaattggag atgaggtaaa aatggaaatt tacaaacaaa gaatgattga
      181 agaatataaa cagttaaaaa aacgcgcaga aaaactatct attgtgctaa acagatacta
      241 ccttgatgag ttagattttg aattatcttg cccaattgaa cttttacaaa cgcaatggca
      301 tattatggga gcgtatttga aaattctaga acaaagattt ttggttgaag ggatttattt
      361 taatgattga attttttttg cctatggata aaatcccgac gacaactcat cagcaaaaga
      421 aagtgactgt cataaatggc aagcctcact tttacgagcc tgagagtttg aaaaatgcta
      481 gggataagtt tacaagtctt ttggctcagc atgtaccacc cagtaagtta gatggtccta
      541 tccgtctgac agtcaagtgg ctatttccca aaatcaaggg cagcacaaac ggacagtaca
      601 aaacgacaaa accagatacc gacaacctgc agaagctgct taaagactgc atgaccgaat
      661 tgggattttg gaatgatgat gcgcaggttg cttcggaaat tattgagaag ttctgggctg
      721 atacagtagg aatatacgta aaggtggaag agttatgaaa attgattata ttaatttttt
      781 tgaacaagtg gttcctgact ggatgagaga gagtaatgtc aaaatgcaag aagttggatt
      841 taatactgaa gcgtattggc aatgggcaaa tcaatctatt gttgcaatct gcgaaaagta
      901 cggaaatgac agtctgatca atgggcagtt tcatctgatt tgggaatggt tggaggataa
      961 agcaaacgga ggttaacgat gactgaagag caagaactaa taaaaccgct gctagaagag
     1021 atagagcgga aagatgatag attgtatatg cagtctaatg taattgccgt cttaatgaca
     1081 gcagttctta tgttactgat gataagtata gccttacaaa accactacga gccgcaaatc
     1141 acaggactac gtacacagct aagtaggaca caaaagcagc ttaaacgtgc tagcgaagat
     1201 aggaccagac agactaagcg gattgcggaa ttgacaggaa atgggggata aggatggatt
     1261 atttaatgat tggaataata gtattatcag tagtaatttt tataatggga tttttcatac
     1321 tttactatca agcaataatt gttaattcga tgaaaaatga ttttcgtaga atgcgtaaag
     1381 aattgagaga agaactcggt tttgatagct ataactggtc tgacagtttc agaaatatgc
     1441 gtagagatat taaatcaaat aaagagttaa tatccgaaat aaactctttg caaatcataa
     1501 aaaaagccaa agaagctaaa cgactagaag aactcgagca gacaaaagca aacgtcgaaa
     1561 aagaaatcaa taagttgaat gggggatgaa gtggtatctg gatgtttagc tttaataagc
     1621 tgtttagcta aaattttagt tgctgttgtt atattaagtg tttttgcgac tattctaatt
     1681 gtaatatctt gcatactgta ttatatagcc gccgggatcg tttcgtttac ttttagcaag
     1741 acgatagata aaacaaaaaa taagtgaggt aggtacaaaa tgaaaaacga aaatttttta
     1801 ggttatgatc tagctgaact attagaagaa aaattagtag ggaaacgact taaatcaata
     1861 gacgacgaaa agattgtact tgccgatggg acagtaatta aaattgaatt aaacgagggt
     1921 tgtggaggtt gtggaaatgg gtggtcagaa ttaaacatca cgacagaaaa cccaaacttg
     1981 gaatctgctg taatgggtgt tgaatacaca gaaaaatata gtgaatggga tgatgaattc
     2041 aaaatttttg tctacatgac agacaattct gttattgaga tttacggata tgacggcgtt
     2101 ggtaatggat attatggata tggtttctgg gttactgtga agaatcctgg tgatctaata
     2161 gaatctgaat tggaggacta atgctaacta caaatcaaat acacaaactg ctgggagtaa
     2221 aagaagtcta caaagcgcct gacacgttga taaaaataat tttagacaag gaaaaacgcg
     2281 aggacttatt cagacagttt ttgaaatatg aaacagatgt atcttacgac tggtttatgc
     2341 aatattttga ggaagaacaa gctgaccgca aaaataaaaa gcaagacttt acgcctaaat
     2401 cggttagtac actattatct aaaataataa gtggtaatca atactacgag gtagcagtcg
     2461 ggacgggtgg gatacttatc caagcatggc aagaacaacg attaaatgac agtccattta
     2521 cttatcgtcc gagtaaatac tggtatcacg tagaagagtt atcggacaaa gcagtaccgt
     2581 ttctactctt taatatgtct ataagaggga taaatggtgt ggtggtgcat ggcgactctt
     2641 taacaagaca agttaaaaac atttattttt tgcaaaatac aaaagacgac atgctgagtt
     2701 ttagtgatat taacgttatg ccaaggactc aagatattga gcgagaattt aatgtcaaag
     2761 agtggattgg tgatgggata gaacacattg aaaatccact aattgaatgg atataaccaa
     2821 taacggtatc ccgcacgttc gactcgtgcg gttggtattg accgaaattg aaaaatagaa
     2881 acgaggacct ccttacacca aaacaaaatc taacgctggt tatcggtcac tggctattat
     2941 ttaaggcgct aatactgaca tcgtacgcct gtgtcaaaaa acaaaggaaa gagaggactt
     3001 ttctccacaa aaaaagacgt ccatgcgaac gcctccatga ttaaataccc aacaatatta
     3061 tatcatgaat ggagagttag atgggcaaca ttccgacaac aaaagctaat aattttttgg
     3121 aagaattaaa gactatccca catctcatag agacccttga aagagacgct aacctaatga
     3181 gtcggtcgct tgttaagtct cctcagtggt ctgatatgcg agtgtctggt ggggttaaac
     3241 aatcacagga agacaaaaac ataaagatgc tgcatatggt tagctattat agtgatcaga
     3301 ttgagcgttt aaaagaccgt cgacaagaaa tggctaattt gattgtgcaa agcatgggca
     3361 tttgtgagag tcacgtttta ctcacgactt atcttgactg tgatggagac tatgagagag
     3421 ccagagaacg cttaaacata ggaaatcgta ataaatactt tatgtttgtt aggcgaggaa
     3481 aggaaagtct ggaattgata ctaaaaaata ctaattagat acaaattgat actacataat
     3541 actaatagct gtgttaatat tgtagtatag caaaatagca agaagagata accttttaac
     3601 caattggcta tttatctatg gttaaccaat tagtcatcac attgtggtga ctttttatta
     3661 tgtaaaaaag gaataaaaca tggagtttgt ggacaaaaaa ttaagtgaaa taacacctta
     3721 taaaaataac cctagaaaca atgatgaggc tgttggacca gttgctgaat ctattaaaga
     3781 gtttggtttt aaagtgccta ttgtcgttga taaaaacggc gaaatcgtaa acggccacac
     3841 tcgttataaa gcagcccaaa aattagggct agaaacagtt cctgttattg tcgcagatga
     3901 cttatctgaa gagcaaatta aagcgttcag acttgctgac aataaagttg gtgagattgc
     3961 ggatgcttat gaggttaaag ccactcgtga gcgcaatggt aattactcac tgtacatcaa
     4021 atatccgcta gatggtgtct ttgcctcagt ttttaaagag gaaatgaaga ttaagtctga
     4081 cgctggtcgt agaaccaaat ggcagacttt tgagattaat cgggtactac gaaatagtaa
     4141 agaccacatc gagatttttg cgcgtcatat ctctatgcgc acacaggata ttgctttaaa
     4201 accgtttgta aacggtgcga gcgtaggagc cgaatcagct ttagaaatct ggaagaaaaa
     4261 ccttgtcggt gatgataagt ttgacgttaa aagcgacatc ttaacgcttg gtagctttaa
     4321 ctgggaaatt gataaaatcg gcaatgcccg tggtgctcta ggaggtgtcg ctggctctat
     4381 cctagatgtt tacggtggtg agtacgagtt tgacaaccgt acaatcatct tacgcaagca
     4441 aatggggcgt aaagctccca cggtattgga gtatggccgt aatatcgtca gcgtagagga
     4501 ggagcgattg ctagatggca attacacctc tatctatcct tacgtaagat atacgccaca
     4561 accaaaaccg caagaggaac cccctggtaa gccgcatgta ggcgagcata aacaacccga
     4621 agaacagcta gtgacattgc ctgaatttat cctagatggt cagtatctca gcttatatgc
     4681 tcagcgcaga atccaaatgg ttgatttatc aagtcatttt aacgatgaca aaaataaaaa
     4741 agagccaacg atcgaagaaa tccgaaagct ggctcagaaa taccttaagg ataataacgt
     4801 tggtgcacca aaagtcagca ttgaggttga ttatattgac ttgtcacaaa cgcttgacta
     4861 tcaagatttt agagtcatgg aagaggttga gctttgcgac attgtaccac tttattatcc
     4921 aaagtttggc atcacaactg agtctgaaaa agtcgttgag attgtctatg acgtctatac
     4981 agatagcaac catacaataa aacttggcac gattggtcag tccatctcta agagtttgac
     5041 aggtggggtc tcagagcgta tcaatgcttt ggaaaataac caaaaggtta tcaccaacag
     5101 ccagaagcag tttgaactca atctgcctaa atacctcaat gacctcaatg gtaaacgcgt
     5161 ttggtacgaa aaaccagatg acaatattga gcacaagata ggcgactatt ggtttgaaaa
     5221 aaatggcaag tatcagcgta cttggatttg ggatggcaat caatgggtca aggtactaga
     5281 tacagaggat ttaaacccca atcaacgagc ttttgatgcc gcaatagccg aactcgaaaa
     5341 agcaaaaaaa gcgcaagaag aaatcaacca gcgtactgac aaagagcttg aagaatttag
     5401 agccaccctc aaaaacctag ctttaccaga agaagcgatt aaaaaaatca cagaggctat
     5461 caaagttgat gacatcccgt ctattaaaca aagctttgat gacctcaaaa atagagtgag
     5521 tgagacaagc gaagaatctc gtttaactgc cgaaatttta ggaaacaacg gtaaaacccg
     5581 ctacaacaaa aatttattgg ttggtgagcc taatcgcacg aagtcttacg accaggatta
     5641 catcgaggta gaagccaacg acggtggttt caagcgtggc gagacctata cgattagctt
     5701 tagccagaca tgtgagccac tcaaaaaagt ggctgtcacg ctgacacagg ctaacaacaa
     5761 gggagttaag ttagtgctga cacctaccaa ggctaagatg gaagcacaga cgtttgaggt
     5821 cgctaaggat aaacagtcta tagaggttta tcctttgagc tacacggctg ttttaactgg
     5881 cgactggtat aaatctaagc agatagattt aaacgcgtcg gaggtgaggg aattggctct
     5941 ggagatggat tataaagaga ttgcagatgc caaaggtgca actatcacag gggcatggtc
     6001 agacagtcca caaattatat tagacggagg taaaaaatga gtgaaaatat accgctgcga
     6061 gtccaattta agcggatgac tgctagcgag tgggctcgta gtgatgtcat cttactggag
     6121 agtgagatag gctttgagac agacacaggt tttgccagag caggtgatgg ccacaatcga
     6181 tttagtgacc ttggatacat tagcccactc gattacaatc tactgactaa caagccaaat
     6241 atcgatggat tagcgacaaa agtcgagacc gctcagaaac tacaacaaaa agcagataaa
     6301 gagaccgtct atacaaaagc tgaatcgaag caagagcttg acaagaaatt aaatctcaaa
     6361 ggtggcgtta tgacaggtca actaaaattt aagccagccg ccactgttgc ttattcctcg
     6421 tcaacgggtg gggcggtcaa tattgacttg tcgtctagca gaggtgctgg tgttgttgtc
     6481 tattctaaca atgataccag tgatgggccg ctaatgagct tgcggacggg taaagagacc
     6541 ttcaataaat cggcgctttt tgtcgattac agcggtaaga ctaatgccgt taatattgcg
     6601 atgcgccagc caagcacacc taatttttcc tctgcgctta atattactag cggcaatgaa
     6661 aatggtagtg cgatgcagct acgagggtca gaaagagcgc taggaacgct aaaaattact
     6721 catgagaacc caagtcttga agcgaattat gataaagatg cggcagcgtt atctattgat
     6781 atcgttaaaa aacagaaagg cggaaaaggt actgctgctc aaggaatcta cattaactca
     6841 acatcaggca cgacagggaa gttgcttagg attagaaacc ttaatgatga taagttctac
     6901 gtcaagcctg acggtggttt ttatgccaag gaaacttcgc agattgatgg caacctgaaa
     6961 ctcaaggatc ccatagcgaa tgatcatgcg gcaaccaaag cttatgttga tggtgaagtc
     7021 gaaaaattaa aagcactctt aacggctaag caaatgtaaa aaggaggaga tatgagtaga
     7081 gatccaacgt tgacattaga cgagtcaaat ctcgttattg gtaaggatgg acgtgtgcat
     7141 tatacattta ctgctagtca agacaaccaa acagttagac tagccagcaa ctgcttaggc
     7201 acagcgcact ttaatcagct catgattgag cgaggggata aagcgactgg ctatgtggcg
     7261 cctgtggttg tcgaggggac aggtaatccg actggactat ttaaagacct caaagagatt
     7321 agcttagagc tgacagatac tgctaattcc cagctttggt caaaaatcaa gctgactaac
     7381 cgtggtatgt tgcaggaata ctacgacggt aagatcaaga ccgagatagt caactccgcc
     7441 agaggtgtcg ctacacgtat cagcgatgat actgataaaa agctagcgct catcaatgac
     7501 accattgatg gtatcaggcg tgagtatcga gatgctgata ggaagctatc cgcaagctat
     7561 caggcaggca tcgaggggct aaaagccaca atggccaatg ataaaatcgg tttacaagct
     7621 gagattaaag cctcagcaca agggctatcg caaaagtatg atgatgagtt gcgcaagcta
     7681 tcggctaaga tcacaacaac ctcaagcggc actacagagg cctacgagag taagcttgcg
     7741 ggcttacgtg ctgagtttac tcgctcaaat caaggcacga ggacagagct cgagtcacaa
     7801 attagcggac taagagcggt acaacagaca accgctagcc aaatctcaca agagatacgt
     7861 aaccgcgaag gtgctgtcag ccgtgtgcaa caggacttag ctagctatca gcggcgattg
     7921 caggacgcag aggataatta cagtagctta acccatacaa ttagaggttt gcagagtgaa
     7981 gtgggatccc cgactggtaa aatccaatca cgccttactc aactagcagg acaaattgag
     8041 cagcgggtta ctagagatgg tgtcatgagt attattagtg gcgctggaga cagcattaaa
     8101 ttagctatcc aaaaggctgg cggcattaat gccaaaatgt ctggtaatga gattatctca
     8161 gcaattaacc tcaactccta cggagtaaca atcgcaggta aacacatcgc tctcgatggc
     8221 aatacgacgg ttaatggcac ctttaccaca aaaatagccg aggctatcaa gattagggct
     8281 gatcagatta ttgcaggcac gattgacgct gctaggatta gagtgattaa ccttaacgca
     8341 agtagtatcg ttggtttaga cgctaacttt atcaaagcta aaattggcta tgctatcact
     8401 gatttgctcg agggcaaagt catcaaagct cgtaatggcg ctatgcttat cgaccttaac
     8461 agcgctaagc ttgattttaa cagtaacgcg accatcaact ttaacagccg agataacgct
     8521 ctagtgcgta aagacggtac acacactgcc tttgtacact ttagtaatgc cacaccaaaa
     8581 ggttatacag gttcggcact atatgcctct attggtatca cctcatctgg tgatggggtc
     8641 aacagtgcgt catctggacg ttttgcgggt atgcgttgct ttaggcacgc tacaggctac
     8701 aaccacactg cggcagtcga ccagacagaa atttatggtg atagtgtact tattgcagat
     8761 gactttaaca tcaatagagg ctttaagttt agaccagaca agacgaaaaa aatccttgat
     8821 atgaacaact tgtatgatgc tgttgttgca ctcggacgtt gctggggaca tctgaggaat
     8881 gccgggtggg ataccgctcg cagcaatttt atagctgctg tcactagaga gttgagtaat
     8941 tacattgata ttatttaaaa ggagacaaca tggatttaac actaaaaaat aaagacttaa
     9001 acacgtttta tagcgtactt gacaaaatca aaatcactaa tatgcgtgcc aaccgtggac
     9061 gtgccaagct actggcaaaa gtggtcgata aaatcaacga gtacgccaag gatgaggctg
     9121 acattattga cttgtacgct ctaaaaaata aagacggcaa gtttgtcatc gacgagcata
     9181 aaaatatcaa gattgaggac ccaaaaaaaa tcgacgagct caacgactta ctgacagagc
     9241 ttggtaatga ggacatcact atcaaaggcc atgagtattc aaaacgcttt atcgatttct
     9301 tggaatatct agccgaatca gaagatgaat ttacatcaga ggaaatcgtc ttaattgata
     9361 atattttgga acaatttgaa gaaagtaaag gagaataatt atgcgcaatt ggaaagtaac
     9421 agggaaatat ccacaatatg acagcacagg agcagtcgca agcacacata ttattatcac
     9481 tgctgaggat ggctcagtca tctctcaacc aatcaagcag gacttaacct caactaatga
     9541 cacagagatt atcaaagccg ctttggaaga atttaaaaaa tctgaatacg ttgaaattgc
     9601 tatgggcgaa gcagtccaaa aagttgatga cttggaaaaa atctcacaag aaaccgctaa
     9661 gactgctaaa acagcccaaa cagccgctgg actagctaag gtgtccgcag agcgtacaca
     9721 gcggatgatt aacttgcaaa ccatccacgt attgacaagt ggtgggaaag ttgaacctga
     9781 tatctacaaa ggtatgttag agctaatcga gccagccaaa aaaggtgagt atcaagctta
     9841 tgacgtgttt acggtggttg acagtactaa agaggaagat ggtgaagcag gcgaagggaa
     9901 cttagtcttt gtacatgtta acgagccatt tacttatgag gcacagacct tagaggagct
     9961 tgagtcagag gacaaagtaa cagtcatcaa atatgcggaa ttagttaaac aagattaggg
    10021 gtggttagat gcactttgat ttattgcaga taggagctgc gtgtaccgct ctcttgtcta
    10081 tcttgggtgt ttggggattt atcgtcaacc cctttaaaaa agccatggaa gctaatgagt
    10141 ttgccatggc tcagctcaag gactcaatta aggagttagc ttatgagctt aaaaaccttg
    10201 atcgtgaccg tgagattacc aaaaaaatta tcgatcgcca cgaagagcgt ttagggcgtg
    10261 tcgaagacga agttattatc aacaaagaac gtattattac gctatttaaa aaaggagaag
    10321 aaaaatgaat aaatggttta aaaaagtagc aatcaaaaca atcaaaacaa tggcacaaac
    10381 agctgttggt cttatcgggt caagcgtgtt gattacggat attaactggc caacgatgtt
    10441 gtcagcggta ttactatcag gactaacttg tgtcctgatg aatgtgtcac aaattaaaga
    10501 agaggagtaa ccatgagaaa agcaatcaca caactagccg tcatcatagc tatcatagtg
    10561 ctatattttc cactggccgt gattgctttg attttggctc catttatagg agaggatgat
    10621 agatggcatt tttagacaaa ataaaaagcg cagttatcgc agagtggcac actcataaga
    10681 ttttgccatc tctgacagct gctcaagcca ttttagagag cgggtggggc aaacatgccc
    10741 cacacaacgc tctgtttggt attaaggcag atagctcttg gactggtaaa tcatttgata
    10801 ccaaaaccca agaggaatat caagcaggtg ttgtcacgga tattgtggac cgatttaggg
    10861 cgtatgatag ttgggatgag tcgatagctg atcacggaca atttttagtt gataatccac
    10921 gctatgaggc agttattggg gagactgact ataaaaaggc ttgttacgct attaaagcag
    10981 ctggatacgc tacggcaagt agctatgtcg aacttttaat ccaactgatt gaggaaaacg
    11041 acttacaaag ttgggataga gaagctctta aaaataataa ggaggaaacg atgacaaccg
    11101 caaacgaaat tgtacaatac tgtgttaacc ttgctaattc aggcatgggt gttgacaaag
    11161 acggtgctca cgggacgcaa tgctgtgact tgccttgttt tgtcgctaaa aattggtttg
    11221 gtgttgatct ttggggcaat gcgattgatt tattagacag cgcaagtgcg caaggctggg
    11281 aagtccatcg tatgccaaca gaggcaaacc caaaagcagg cgctacattt gtccaatcag
    11341 tgccgtatca tcaatttgga catacgggaa ttgttatcga ggatagtgac ggttacacca
    11401 tgcgcactgt cgagcaaaac attgatggca atcctgatgc tttgtatgtc ggtgcaccag
    11461 ctcgttttaa cactcgtgac tttactggcg tgataggttg gttttaccca ccatatcaag
    11521 gggatacagt cacgcaacca gtcagcaccg agccgcaaac ttctgacact atcgtagaga
    11581 cagcaaaaac aggcaccttt acccttgatg ttgcagagat caatatcaga cgctggccaa
    11641 gtctagccag cgaggttgta ggtatctaca agcaaggtga tactgtcagc tttgatagcg
    11701 agggctacgc taatggctat tattggatta gctatgttgg aggctcaggt atgcgtaact
    11761 acctaggtat tggacagact gataaagatg ggaatcgcat cagcctttgg ggtaaattaa
    11821 attaggaggt aaagctccga gataagacaa aaccgctcag attattctgg gcggtttttt
    11881 gtgtatgatg aattattttt caagataaat atcgaaatga ctaaagttct tcatattgat
    11941 aattctatta tctttatatt ttgcaaaaat atctgatcta gtcccttcat ttggtgagtc
    12001 aaataagtct atttgctcat gtttcccatc ttttgtgcca a
//
LOCUS       DAUGFP010000047        11140 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.047, whole genome shotgun sequence.
ACCESSION   DAUGFP010000047 DAUGFP010000000
VERSION     DAUGFP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.047"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..466
                     /locus_tag="VH663_001366"
     CDS             <1..466
                     /locus_tag="VH663_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ8174562.1"
                     /translation="ALLQKKEYSKITVRDMITLANVGRSTFYAHYESKEMLLKELCEE
                     LFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSNDPYFLLRFKNELEHDVYPN
                     LRCKYIDKTTIPEVFLKQFVLSSFIETLKWWLHQRQRMSANELLKYYLELIR"
     gene            681..1199
                     /gene="rimM"
                     /locus_tag="VH663_001367"
     CDS             681..1199
                     /gene="rimM"
                     /locus_tag="VH663_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEQ8174563.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DRFVQEVTIVSHRKQKHFDIIKFKDMYHINAIEKYKGYTLKVSKANQGDLQEGEFYYH
                     QIIGMAVYEKGRLIGYVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            1189..1920
                     /gene="trmD"
                     /locus_tag="VH663_001368"
     CDS             1189..1920
                     /gene="trmD"
                     /locus_tag="VH663_001368"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEQ8174564.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALGQGED"
     gene            1920..2912
                     /locus_tag="VH663_001369"
     CDS             1920..2912
                     /locus_tag="VH663_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ8174565.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGAELTENLIKQLSRFEDHTTICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLKASTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEMLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            3090..4148
                     /locus_tag="VH663_001370"
     CDS             3090..4148
                     /locus_tag="VH663_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEQ8174566.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNKFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFED"
     gene            4161..5084
                     /locus_tag="VH663_001371"
     CDS             4161..5084
                     /locus_tag="VH663_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEQ8174567.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     VVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPNNQDEGHQV
                     ADLLNKANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
     gene            5340..6053
                     /locus_tag="VH663_001372"
     CDS             5340..6053
                     /locus_tag="VH663_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ8174568.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKTIAQRASQFIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGVDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            6050..6961
                     /gene="pfkB"
                     /locus_tag="VH663_001373"
     CDS             6050..6961
                     /gene="pfkB"
                     /locus_tag="VH663_001373"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEQ8174569.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETELN
                     GQGPIISQEQLEELKTKLSQLTAEDTVVFAGSAPANLGNAVYKELLPLVRQSGAQVVC
                     DFEGQTLIDALTYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNAIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            6958..8904
                     /locus_tag="VH663_001374"
     CDS             6958..8904
                     /locus_tag="VH663_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ8174570.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSET
                     AVSTEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVAIGAIVSGILFGALRKKA"
     gene            8996..9595
                     /locus_tag="VH663_001375"
     CDS             8996..9595
                     /locus_tag="VH663_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ8174571.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDPLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYHTKLNELIEWYQLTNFDGLMK"
     gene            9746..10453
                     /locus_tag="VH663_001376"
     CDS             9746..10453
                     /locus_tag="VH663_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ8174572.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNAYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
     gene            complement(10510..10821)
                     /locus_tag="VH663_001377"
                     /pseudo
     CDS             complement(10510..10821)
                     /locus_tag="VH663_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(11025..>11140)
                     /locus_tag="VH663_001378"
     CDS             complement(11025..>11140)
                     /locus_tag="VH663_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ8174573.1"
                     /translation="YDAKPNSTRSDVLKKYKDNKTINMKDFSHFDIYLWTK"
BASE COUNT         3419 a         1821 c         2376 g         3524 t
ORIGIN      
        1 tgctttatta caaaaaaaag aatattcgaa aataactgtt cgagatatga ttacacttgc
       61 taatgtaggg agatcaacct tttatgcaca ttatgaaagc aaagaaatgt tactaaaaga
      121 actctgtgaa gaactttttc atcatttgtt taggcaaaaa agaaatgtga cttttgagga
      181 ctatttggta catattttaa agcattttga acaaaataaa gacagcattg cgacgttgtt
      241 actttctaat gatccctatt ttttattaag atttaaaaac gaactagaac atgatgttta
      301 ccctaacttg cgctgcaagt atatcgacaa aacgactatt ccagaagtat tcctgaaaca
      361 atttgttcta tctagtttta tagaaacctt aaaatggtgg ctccatcaaa ggcaaaggat
      421 gtctgcaaat gaactattaa agtattattt agagctcata agataatgac tagtgagtca
      481 tcttgccaaa ttcaattttt taatacagac ggattttaac aatttaacag agaaagacaa
      541 aactgtaagt gcaaaataga ctataacgtg ataaaataat aggtaatttg gttctgaaaa
      601 agaagtgtct atcaaacttt agaatcaaga gaggtcgcta aagtgacgtg agataaagag
      661 ttaacgattt aggagctacc atggaatatt ttaatgttgg aaaaattgtc aacacgcaag
      721 gcctgcaagg agagatgcgc gtgttgtctg tcagtgattt tgctgaggaa cgctttaaaa
      781 aaggctcaca attagcttta tttgatgata aagatcggtt tgttcaagaa gtgacaattg
      841 ttagtcaccg taaacaaaag cattttgata ttatcaaatt taaagacatg tatcatatta
      901 atgcgattga aaaatataag ggctatacgt taaaagtgtc aaaagctaat caaggagacc
      961 tgcaagaagg agaattttac taccatcaga ttatcggtat ggctgtttat gaaaagggcc
     1021 ggttgattgg ctatgtcaag gaaatcttgc agccaggtgc taatgatgtt tgggtagtga
     1081 aacgccaagg aaaacgagac ttgctcttac catatattcc cccagtagtt cttaatgtag
     1141 atgttcctaa caagcgcgtg gatgttgaat tgatggaagg cttagacgat gaagattgat
     1201 attttaaccc tttttcctga aatgtttgcc cccctagaac attccattgt aggcaaagca
     1261 aaagaaaaag gattacttga cattcactac cataactttc gagattatgc tgaaaaagct
     1321 cgtcatgtgg atgacgaacc ctatggtggg ggccaaggca tgttattgag agcacagccc
     1381 atttttgaca ccattgaaca gattgaagcc aaaaaaccaa ggattattct tttagatcca
     1441 gcaggtaaac cctttactca ggcttatgct gaagaattag ctcttgagga agagttaatt
     1501 tttatttgtg gtcattatga aggttatgat gagcggatta agaccttggt gacggatgaa
     1561 atttcattag gtgactttgt attaacagga ggtgagttgg cggccatgac tatggtggat
     1621 gcgaccgtca ggttaattcc acaggtactt gggaaagaaa gtagccatca ggacgattct
     1681 ttttcatcag gacttttaga ataccctcaa tacacaagac cttatgatta tcgtggtatg
     1741 acagttccag atgttctaat gagtggtcat cacgaacgta ttcgcttgtg gcgtctagaa
     1801 gaaagtctca gaaaaaccta tttgagaagg ccagatttgt tagagcggta cgatttttcc
     1861 gaggaagagc gcaagctatt ggataagatt aaagaagcat tgggtcaagg agaagattag
     1921 tgaaagataa agcatatgat attacgatta ttggtggtgg tccaataggt ttatttgcag
     1981 ccttttatgc aggtcttagg ggcgtaacag taaagattat tgagagtttg tcagaacttg
     2041 gtggacagcc tgccattctt tatcctgaga aaatgattta tgatattcca gcttatccat
     2101 ctttaacagg tgctgagttg acagagaatt taataaaaca attaagccgt tttgaagatc
     2161 atacaaccat ttgtttaaaa gaagaagtat taacctttga caaggttaaa ggtggttttt
     2221 cgattaggac taataaggca gagcattttt caaaagccat tatcattgct tgtggaaatg
     2281 gcgcttttgc accaagaact ttagggttag aaagtgagga aaactttgct gatcataacc
     2341 ttttttataa tgttcaccag ttggaccagt ttgcgggaca aaaagttgtg atttgtggtg
     2401 gtggagattc agcagttgat tgggctttgg ctttagaaga tattgctgag agtgtgacgg
     2461 ttgtgcaccg tcgtgatgct ttcagagcac atgaacacag tgtggaatta ttgaaagcat
     2521 caacggtcaa cctcttaaca ccttatgtgc caaaagcatt gaaaggaatt ggaaacctgg
     2581 ctgaaaaatt agttattcaa aaagtgaaag aagatgaaat gttagaactt gaattggaca
     2641 gtcttattgt tagttttggt ttttcaactt ctaataagaa tttgaaaaat tggaatctcg
     2701 actataagcg ttcaagtatt actgtttcac cactttttca aacaagtcaa gaaggtattt
     2761 ttgccattgg tgatgcagcc gcttataatg ggaaagtgga tttgattgct actggttttg
     2821 gcgaagcacc aacagcagtt aatcaggcca ttaactatat ttatccagat cgagataacc
     2881 gtgtggtgca ttcaacctct ttaattgact aagggagtcg gaagtttcaa gagcagtagc
     2941 aacagctaat attcagtttg tcaggagagg acaaatggtt tttacctaag tcttcgtgac
     3001 aagttttata ctttgtgata aaatagtcat tgtgaaaaaa gtgtgatggt tttcacagat
     3061 taacacatta caatgaaaag gggtctatca tgacaacaac agataaagag acatttagtt
     3121 cttttatgaa taaggtcttg gctggtacag cgattgctat cgttgtggca ctcattccaa
     3181 atgctatttt agcgactttc ttaaaaccgc ttttgccaaa catggcagct gctgaatttt
     3241 tacacattgt gcaagtcttc caattcttca cgccgattat ggctggtttc ttgattgggc
     3301 aacaatttaa gtttaatcct atgcaacagt tggctgttgg tggagctgct tatatcggtt
     3361 ctggggcttg ggcttataca gaagtcattc aaaaaggtgt ggcgacagga acattccaac
     3421 ttagaggtat tggggactta atcaatatga tgattacagc tagccttgcg gttttagctg
     3481 tgaaatattt tgggaataaa tttggctctt taaccattat cttgttacct atcactatag
     3541 gaactggcgt aggttatatt ggttggaaat ttttgcctta tgtgtcttac gtgacaaccc
     3601 tgatcggaca aggtattaat tcttttacaa ccttacaacc tattttaatg tctattttaa
     3661 ttgcagttgc tttctcgctt atcattgtta gtccgatttc aactgttgca atcggtttag
     3721 ctattgggct taatggaatg gctgcgggag cagcttctat gggaattgca tcgacagcag
     3781 cagtcttagt gtgggcgacg cttaaagtta ataaatctgg tgtaccaatt gctatcgcac
     3841 ttggggctat gaaaatgatg atgccaaact tcttgaaaca tcctatcatg gctattccga
     3901 tggtgttcac tgcagcaatt agctcattaa cagtaccatt gtttaatctt gttggaacac
     3961 cagcttcatc tggttttggc ttagttggtg cagtaggtcc gattgcttct ttagcaggtg
     4021 gtagctcaat acttattatt atccttgctt ggatcattgt tccgtttgcg gttgcttttg
     4081 cggcgcataa ggtttctaaa gatattctaa aactttataa agaagacatt ttcgtctttg
     4141 aagactaata ggaggaaaat atgttagttt atattgctgg ctcaggagcc atggggtgcc
     4201 gttttggtta ccaaatttca aaaacaaata acgatgtgat tttgttagat aactgggaag
     4261 atcatatcaa tgccattaaa gaaaacggtt tagtggtgac aggggatgtg gaagaaacag
     4321 ttaagcttcc tattatgaaa ccgactgaag ccactcaaga agccgatttg attatcttgt
     4381 ttacaaaagc aatgcagttg ccacaaatgc ttcaagacat taaaggcatt attggaaaag
     4441 aaaccaaagt gctttgcctt ttaaatggtc ttggacacga agacgttatt cgtcagtaca
     4501 ttccagagca caacattttg atgggggtca ctgtctggac tgctggtcta gaaggaccag
     4561 gtcgtgctca tcttcaagga gttggtgctc ttaacttaca aagtatggat ccaaacaatc
     4621 aagatgaggg acatcaagta gctgacttgc taaataaagc taacttaaat gcgacttatg
     4681 atgagaatgt tgtgccaaac atctggcgaa aagcttgcgt gaatggtacc atgaattcga
     4741 cttgtgctct tttagattgt acgattggcg aattatttgc cagtgaagat ggtctcaaaa
     4801 tggtaaaaga aatcatccat gagtttgtta tcgttggtca agcagaaggt gtcgaactaa
     4861 acgaagaaga aatcacgcaa tatgtaatgg atacatctgt taaagctgct caccattacc
     4921 catcaatgca ccaagacctt gtgcaaaatc accgattgac tgaaattgat tttatcaatg
     4981 gtgcagtcaa taccaaaggt gaaaagcttg gtatcaatac tccatactgc cgtatgatta
     5041 cagaactagt tcacgctaaa gaagcagtgt tgaacatcca ataaacgcta tcaaatcgtt
     5101 atactcaata ttatggctaa agctttaata ttgagttttt tgttatgctg atagtatttt
     5161 tcttaggcgt cattatttgt gagcctagat tatgagttaa taaaagaaat aataattggt
     5221 agaatttgat agattttgaa aggttatgct tgacttttag ttataaaata gtataataat
     5281 tgtaaacgat ttcagttagg ggtggttcta tcttaaaatc aaaacgaaaa caattaatca
     5341 tggcaaagat cactgaagaa aactatgttt ctttagagga tttaatgcaa ttactcaact
     5401 cctctgagtc tactatccgt agagatctag gagagttgga gcaagaagga cggctacatc
     5461 gtgttcatgg aggtgctgaa ctttttcact ctttacaaga ggaactttct aaccaagaaa
     5521 agtctgtcaa aaacagtcat attaaaaaaa ctattgcgca aagagcctct cagtttatct
     5581 atgacaacga tgtgatcttt attgatgctg ggacaacgac tgaattttta ttaccttttt
     5641 tacaagctaa aaatttaact gtagttacta attctattca tcatgctgct agattagttg
     5701 aactctctat tgagacgatt attgtagggg gttatgtcaa acaaacgacc gatgccagta
     5761 ttggtaatgt agctttggaa cagattaggc agatgaattt tgacaaggca tttcttggaa
     5821 tgaatggtgt tgatgattct tacctgacga ctcctgatat ggaagaagca gttattaaaa
     5881 aagcggttct gtcaaatgca aaattagctt acattttagt agatggcact aaaattggtc
     5941 aggtatcgtt tgtaaaagta gctcctatta atgatgtgac tattattaca ttaggaggat
     6001 cagcaagtat tcttaaacaa ataaaggaaa aggcaaaggt gattgaatta tgatttatac
     6061 cgtgacctta aacccttcta ttgactttat tgtcagaatt gaccaaataa atcttgggtc
     6121 agtgaatcgc atggcgagtg atgataagtt tgctggaggt aaaggcatta atgtgagtcg
     6181 catcttgcaa cgtttagaca ttgctagcac ggcgacaggg ttcttgggtg gttttacagg
     6241 acgttttatc gaagaaagtt tgagtgcaga aggcgttaaa actgattttg taaaaggtga
     6301 tcaagatact cgaatcaatg ttaaaatcaa gtcacaagaa gagaccgaat taaatggtca
     6361 aggacctatc attagtcagg aacaactaga ggagttgaaa actaagcttt cccagctaac
     6421 agcagaggat acagttgtct ttgcaggttc agcgccagct aatcttggca atgctgttta
     6481 taaggaatta cttcctttag ttaggcaaag cggtgctcag gtggtttgtg attttgaagg
     6541 acagacttta atagatgcgc ttacatataa tcctctgttg gttaaaccta ataatcatga
     6601 actagaagct atttttggta cgattctgac tagtcttgat gatgttgaaa cctatgcccg
     6661 tcgtctttta gagatgggtg ctcaaaatgc catcatttca atggctggtg atggtgcttt
     6721 gctggttact aaagaagcga cttattttgc taaaccgatt aagggagaag ttaaaaattc
     6781 tgtcggtgct ggtgattcga tggttgctgg ctttacaggc gaatttatga agagtcaaaa
     6841 tccaatagaa gccttaaaat ggggcgtggc ttgtggtaca gcgacggctt tctctgatga
     6901 cttggcgaca attgcattta ttaaggaaac atatcataaa gttgaggtag aaaaacgatg
     6961 aaaattcaag atttattgag aaaagatatt atgattctcg atttacaggc tatttctaaa
     7021 gaagttgcca ttgacgagat gattactaaa ttagttgaaa aagatattgt acatgatttt
     7081 gatgtcttta aaaagagtat catgacacgt gaagaacaaa catcaacagg gcttggtgat
     7141 gggattgcca tgcctcattc taaaaatatt gtagtagata aaccagcggt cttgtttgcg
     7201 aaatcaaata aaggcgtgga ttataaggct ttagatggtc aaccaacgga tctcttcttt
     7261 atgattgctg cgcctcaagg agcaaatgat actcacttgg ctgcgctagc agaattatca
     7321 caataccttt tgaaagacgg ctttgctgat aaacttcgtg ctgcagcgac accagaagca
     7381 gtcattgctg tttttgatga agcgtcaaca gctaaagaag aagtggttgc tccaacaagt
     7441 ggtcaagact ttatcgttgc tgttacagct tgtccgacag gaattgctca cacttatatg
     7501 gcagaagagg ctttgaaaaa acaagctgca gaaatgggag tagctattaa agttgaaaca
     7561 aacggtgctt ctggtgtggc taatcgttta accgctgaag atattcagag agctaaagga
     7621 gttattgttg cagctgataa agcggttgaa atggaccgtt ttgatggtaa acaattcatt
     7681 gctcgccctg ttgcagatgg tatcaagaaa agtcaggaat tgatttcttt gattttaaac
     7741 aatgaaggaa acacttatca tgctaaaaat ggaaaatctg aaacagcagt atcaactgag
     7801 aaaacaagtt taggcggtgc tttctataaa cacttgatgg gcggtgtctc acaaatgttg
     7861 ccatttgtta ttggtggcgg gattatgatt gctctagcat ttttattgga taacatgctt
     7921 ggtgtgccaa atgatcagct tggaagtctt ggatcctatc atgaaatagc agctattttt
     7981 atgaacattg gtggagcagc cttttccttc atgttaccag tactagcagg ttatatcgct
     8041 tattctattg ctgaaaaacc gggcttagta gctggttttg tggcaggtgc cattgcctca
     8101 aatggtcttg cttttggtaa ggtaccattt gcagcaggag gagaagtgag cttgggctta
     8161 acgggcgtgc catcaggttt ccttggggcg cttgtgggtg gtttccttgc cggtggtgtt
     8221 attcttgccc ttcgtaaatt gttggcaggc ctaccacgtt ctctagaagg ggttaaatct
     8281 atcctccttt acccattact tggcgtcctt gtcactggtt tcttgatgct ttttgtcaat
     8341 attccaatgg cagctattaa cacagccctt aatgatttct tacaaggtct ttcaggaagc
     8401 tctgctgtcc ttatgggact tcttgttggt ggaatgatgg ctgttgacat gggtggtcct
     8461 gttaataagg cagcttatgt ttttggtaca ggaactttag cagctactgt cgcaaatggc
     8521 ggttcagtcg ttatggctgc tgttatggca ggtggtatgg tacctcctct agctgttttt
     8581 gtagccactc tcttgtttaa agataaattt accaaagaag aacgtgaatc aggtttgacc
     8641 aacattgtca tgggactttc tttcatcact gaaggagcaa tcccatttgg cgcagctgac
     8701 ccagcgcgtg caattcctag ctttattgct ggttctgcct tgacaggagc tcttgttggt
     8761 ttggctggta ttaaattaat ggcgccacat ggtggtatct tcgtcatcgc cttaacaagt
     8821 aatccaatct tataccttgt ctttgtagcg attggtgcta ttgtatccgg aatcctcttc
     8881 ggtgcccttc gtaaaaaagc ttaacgttaa aagacttttt ccttactaaa actagaatct
     8941 gctaaggttc tagttttttg atatactaga gttaaaggat aagaaatgag gacctttgaa
     9001 ggtgaaaaaa aggagaagaa gggctaaatc atcagtaaat cgacttgtct tagggttagt
     9061 attacttaat ctgattgtta gtatgtggac attaaaattg ggaaaccagc gattagctcc
     9121 gtatgcggat cacgaaactc tgacatttgt caggaagatt agtcatgctg ctcaatcagt
     9181 cgctcaaaag aaacagttat acagttcggt gatgatggct caggccattt tagaatccaa
     9241 taatggtaag tcacaactaa gtcaaaaacc ttattataat tttttcggga ttaaggggag
     9301 ctataaagaa cggtcagtca tttttccaac tttagaagac gatggacaag ggaatcttta
     9361 tcaaattgat gctgcttttc gttcctatgg gagcctgaca gcttgttttt tagattacgc
     9421 aagagttttg aatgatccac tttatgacaa aacccacaaa aagttttggt ctcattatca
     9481 agatgctact gcaaccttaa caggcactta cgccactgat acaacttacc ataccaaatt
     9541 aaatgaattg attgaatggt atcaactcac taattttgat ggtctaatga aatagttatc
     9601 cacaatttta aaaaaatcag aagaaatatg tcgatttcaa gacaaaagtt atcaatctca
     9661 ttaaattatc tgtggaaaag ttaatttaaa cgttcgtgtg ctataataga aacatagaaa
     9721 atgtataaaa aaggatatgt gaataatgac aaaaaagaaa ggtaagcttg tacttatcag
     9781 tctttttgtt ctagcagctt gtttaggagc ttatagtgca atgagacaat ctcataaaac
     9841 ttcaaacgtg tcagctgaga ctatcgccag ttcttcaacc cgacatttta ttgatgaaat
     9901 tggtccaact gctagtacta ttggtcaaga acgtgatctt tatgcgtctg ttatgatagc
     9961 acaagcgatt ttagaatcaa gtaatggtaa atcaagtcta agtcaagcac cttattataa
    10021 ttttttcggt attaagggtg cctataacgg ttcttctgtg acgatgtcaa cttgggaaga
    10081 tgatggtaat ggcaatgcct atacgattga tcaagccttt agagcttatc caagtattgc
    10141 agactccctt aatgactatg cagacttgct aagttctagc acttatatag gtgctagaaa
    10201 atcaaacacg ctatcgtatc aagatgctac agcagctcta actggcttat acgctacaga
    10261 caccagttat aacttaaaac taaataatat tattgcaacc tatggtttga cggcttatga
    10321 tgttgctaat agctcagctc aagaaactgg tctagcaact tcgggttatg tttggaatga
    10381 gtatcgtcgt aattacactg atgcggagac cctagcagtt gatgaggctt gggctaaacg
    10441 catgacttat taatgtaaca ctaacaacaa tctgcttgca ctgaccccaa aaaagctgga
    10501 tactctattt taagtaaagg atttagttct gtattgtaca gaactaaatc cttttagttt
    10561 tactttgata tgtttatcgt tgtaataatc aatgtcattt gtaatcgctt attctaagtc
    10621 ttctagtgat ttatagttat tctcataacc atagaacatt tctgacttta agatgccaaa
    10681 gaaagattcc atcatgctat tgtcaggact gtttctttta cgtgacatag acggacgaat
    10741 tcctttagag tttaggaaat gatgataata gttgtgttga tattgccaac cttgatcact
    10801 ctatcatata gttccatcat agcatttatt tggagatgtc tttttccatt attggtttaa
    10861 tgtgatttaa gcaaggttat tagctaaact taatgccaga caaaaacacc ccaattatta
    10921 gattttttgt ctaacttttg gggcgcaatt caatctgagc agttttagat ttggtatcaa
    10981 tgttattata ggtcaattga tcatatttat ccaaagcttt tagcttattt agtccaaaga
    11041 taaatatcaa aatggctgaa atctttcatg tttatagtct tattgtcttt atattttttt
    11101 aagacatcag atctggtgct attgggtttt gcgtcatata
//
LOCUS       DAUGFP010000048        10986 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.048, whole genome shotgun sequence.
ACCESSION   DAUGFP010000048 DAUGFP010000000
VERSION     DAUGFP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.048"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            107..1096
                     /locus_tag="VH663_001379"
     CDS             107..1096
                     /locus_tag="VH663_001379"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ8174574.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILAKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            complement(1205..1996)
                     /locus_tag="VH663_001380"
     CDS             complement(1205..1996)
                     /locus_tag="VH663_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEQ8174575.1"
                     /translation="MTYTSLKSPEKTDYLYDLNIAHLYGNLMNTYGDNGNVLMLKYVA
                     EKLGARVTVDIVSLNDAFDQDYYDLVFFGGGQDYEQTIVAKDLPSKKAALADYIAKNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTALKKKYGSAISLPSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(1996..3339)
                     /locus_tag="VH663_001381"
     CDS             complement(1996..3339)
                     /locus_tag="VH663_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEQ8174576.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGRLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     ERQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFVCLNCQFQRPTLDYQLTELTAITHQSSEFVIDGQNYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITHMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIMEAIEKQESKHAYILATYTAMLEFRSLLADRHVVEKEMN"
     gene            3446..4297
                     /locus_tag="VH663_001382"
     CDS             3446..4297
                     /locus_tag="VH663_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEQ8174577.1"
                     /translation="MNNLSSIDIKFLLSLFADPWLLAVHLLDILIVAYLIYRFIKALT
                     GTKIMSLVQGVIFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEK
                     FGRSTQVFLQKQYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPL
                     NADISSQLLINIFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAI
                     GLSENSDALTIIVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKK
                     ILGGKSK"
     gene            4294..5250
                     /locus_tag="VH663_001383"
     CDS             4294..5250
                     /locus_tag="VH663_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEQ8174578.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVSVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVNKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            5304..6659
                     /locus_tag="VH663_001384"
     CDS             5304..6659
                     /locus_tag="VH663_001384"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEQ8174579.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     AKIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            6783..7424
                     /locus_tag="VH663_001385"
     CDS             6783..7424
                     /locus_tag="VH663_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174580.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTQKQDT
                     QNRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLAD
                     MQEKLPSLSIMVSQESSKLFYQEQGFVLHAKGCKPGNENDSDVCQLLKYFSKP"
     gene            7487..8617
                     /locus_tag="VH663_001386"
     CDS             7487..8617
                     /locus_tag="VH663_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEQ8174581.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FDSYGIRDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTITNLKAAGFHNISIDLIY
                     ALPGQTLDQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGMSFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            8627..9379
                     /locus_tag="VH663_001387"
     CDS             8627..9379
                     /locus_tag="VH663_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEQ8174582.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGD
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKLHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCEFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQITSDGQLNAQAMIEWRAIKQTESETD"
     gene            9379..10143
                     /locus_tag="VH663_001388"
     CDS             9379..10143
                     /locus_tag="VH663_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEQ8174583.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQERGIPYLLVTNN
                     TTRTPEMVQSMLANQFHVETSIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            10143..10775
                     /locus_tag="VH663_001389"
     CDS             10143..10775
                     /locus_tag="VH663_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEQ8174584.1"
                     /translation="MVENTKLLCSWGWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
BASE COUNT         3391 a         1939 c         2263 g         3393 t
ORIGIN      
        1 aagtgtaggt actgaagatg tcatacagaa taggacactt gtcttttata tttgttttca
       61 ttatactaga gaggtagatt acatttttga aaggattttt tctcctatga aatacatcgt
      121 taacaaaagt cacaatcctg cctttaacat cgctttggaa gcctatgctt ttagagaact
      181 agtggaagaa gacgaattgt ttatcctttg gatcaatgag ccagctatta ttattggcaa
      241 acatcaaaat accattcaag aaatcaataa agaatacatt gatgaacatg gtattcatgt
      301 tgtgcgacgt ttatctggtg gtggtgcggt ttatcatgat ttgaataact tgaactacac
      361 tatcatttca aataaaacag cggaaggcgc atttgatttc aaaaccttct cacaacctgt
      421 gattgcgact ttagctgatt tgggtgtaac agccaacttt acaggccgta atgatattga
      481 aattgatggt aaaaaaatct gcggtaatgc tcaagcttac tacaaaggtc gcatgatgca
      541 ccatggttgc cttttgtttg atgtggatat gaccgttctt ggcgatgctc ttaaggtaag
      601 taaggacaag attgagtcta aaggagtgaa gtctgttcgt gcccgtgtga ccaatatttt
      661 aaatgaatta ccagaaaaaa ttaccgttga ggaattttca gataaaatct tggccaagat
      721 gaaagaaacg tacccagaca tgacagaata tgtcttgtca gaggatgagt tggctaagat
      781 tgaacaatct gccaaagagc aatttggtag ctgggattgg acctacggca aagcgccaga
      841 atatacgatt gaacgcaatg ttcgttaccc tgcaggtaaa atttcaacct ttgcaaatgt
      901 tgaaaattct atcattaaaa accttaaaat ctatggtgat ttctttggaa tcaaagatgt
      961 gcaagacatt gagaacttat taattggttg taaatatgag taccgtgatg tttttgaacg
     1021 tttaaaaacg attgatacga cccagtactt ctcacgcatg accgttgaag aagttgctaa
     1081 agctattgtt tcttagtggt tttaatcact taagaagctc tgtcagaaga aaaaactata
     1141 aaactcctaa gcgaactatc gtgtcctact taggagtttt tgtttgatga catgaaagaa
     1201 gaacttaaac tttattaaag gaagctttgc ttttaaggtc ggcatattct tcagtgattt
     1261 cttgtttcag tatatcatca tagctaggta gtgaaatagc tgatccatac ttttttttaa
     1321 gagctgttgt tactaagcgg taagcaagat taacattgcg tgataagata ggaccgtgga
     1381 aataactacc atagacgttt ttatagtgaa caccttcagt ttggtcttct ttattattac
     1441 cgttgccata aaccactcgt ccaagtggct tttcgtcacc agataagaag gtacgtcctt
     1501 gatgattttc aaagccataa taggtttcgt taaattcgtc attatgaatt ttaatatctc
     1561 caataaagcg attttggtgc tgattaaggg tgtagtgtcc cataattcca agtccatcaa
     1621 ttttaacccc attagcttgg acatagtatt gccctagtaa ttggaagcca ccacagatgg
     1681 caagcaccac tttattctta gcgatataat ccgctaaagc agcttttttg ctaggcaaat
     1741 ccttagcaac gatggtttgc tcatagtctt gtccaccacc aaaaaaaaca aggtcgtagt
     1801 aatcttggtc aaaggcatca ttgagggaaa caatatcaac ggtaactcta gctccaagtt
     1861 tttcagcgac atacttgagc atcaagacat tcccattgtc accgtaggta ttcatcaggt
     1921 ttccgtagag atgagcaatg tttaggtcat agaggtagtc agtcttttct ggagatttta
     1981 gtgaagtgta agtcattagt tcatctcctt ttcaacaaca tggcggtcgg ctagcaagct
     2041 acggaattct aacattgcag tataggttgc taaaatataa gcatgtttgc tttcttgttt
     2101 ttcaatagct tccatgattt gctcgagctt ttcagcttgt tttatcttag tgtcgtcaaa
     2161 tcctgtgact ctaagtcttc tggcaatttc agagtgacgg acaccaccag cattaatctc
     2221 agtaattggc atgtgtgtaa tgagttcgaa attagcatcc caaatccagc tggtatcaat
     2281 gccatcagcg taattggcat ttaaaagcac agataagcta aatgggtaat cagctagctg
     2341 aatcatttca agcgcttgac tggctccaac aggattcttg ataaggataa gggtgcatga
     2401 tttatcacca atagtaaaag tttcttgtct tccaaaaacc gccttacttt tgttaaagcc
     2461 cgccttgatt ttctcgggag aaacaccgaa aaattcagca acagagacgg cagcaagcgc
     2521 attgtaaata ttataaagac caccaacatt tatcttgtag ttttgtccat caataacaaa
     2581 ttcagaactt tggtgagtaa tagcggtgag ctcagttaat tgataatcta acgttggtct
     2641 ttggaactga cagtttaagc aaacaaagtc tcctagattc gcataagtat tgagacgata
     2701 ctgaagaata tgttcgcatt ttggacataa aatcccttct gtgttgtaat gagctagctg
     2761 cggagcatgc ttagcagtat caaaaccata atactgaact ggattaacta tgtccttaga
     2821 agagaaaata ggactatctc cattggctag aatagttgct tttggagcat ttcgagcacc
     2881 atctacaatc atctgataag tagtataaat ctctccgtag cggtccatct gatccctaaa
     2941 aatattagta taaacaaaaa gactaggttt taagtaagtg gtaattctag gaagactagc
     3001 ctcgtcaatt tctaaaacag caatttgcct ttcagatttc ccttttttag cagctaaaaa
     3061 agtagaagta attccagtaa tcatattggc accacttgga tttgtgatta tctcgccaaa
     3121 tgcctctttc aaaataccga cagtaagagc agttgttaac gttttaccat tagtaccagt
     3181 gacgaccaca atatcatagt ctttagacaa atcctttaaa atatctttat tacaagctag
     3241 agccaatcga ccaggataag tagatcctcg ccctagtttt gttaaaatgg attgagcagc
     3301 tttgccagca ataatgccga gtaatgtctt cattttcatg cttatatttt atcataagtt
     3361 tattgatttt agatagggaa aaatctttga tcatgatatg atataatata gctaataact
     3421 tttttgatat agaaatgaga gttgtatgaa taatttatct agtatcgata ttaaattttt
     3481 attaagctta tttgcggatc cttggctgct agcagtgcat cttttagata ttctaattgt
     3541 tgcttatttg atttatcgtt ttattaaagc attgactgga actaaaatca tgtctttagt
     3601 tcaaggggtc attttctttt tggttcttcg ttttattgca gagtggattg gctttacgac
     3661 aatcacatac ttaatgaacc aagtcattac ttatggtgta attgcaggag tagtcatttt
     3721 tacccctgaa attcgggcag gactggagaa atttggtcgt tctacgcaag tttttttaca
     3781 aaaacagtac gtcagcagtg aatctgcttt ggtcgatgct ttgattaaat ctgtggccta
     3841 tatgggtcca cgcaagatag gtgcactgat tgctattgaa cagactcaaa ccttacaaga
     3901 atatattgcg acaggaattc cgctgaatgc cgatatttca agtcaactac tcatcaatat
     3961 ttttattcca aatacgccgc ttcatgatgg agctgttatc gttggtcaga ataagattgt
     4021 ggcagcttgt gcctacttgc ccttgtcaga atcaaaggca atttccaaag aatttgggac
     4081 ccgtcataga gcggctattg gattatctga aaactctgat gcactaacta tcattgtttc
     4141 agaagaaaca ggagctattt cagtaactcg caaaggccaa tttttacacg atttaagcac
     4201 agatgaattt gaaacggtac ttaggaccta cctgatgagt gattcaaatg ttagtttacc
     4261 atggtataag aaaattctag gagggaaatc taaatgaaac gatttttaaa tagtcgtcct
     4321 tggttaggaa tggtatcagt cttttttgcg attctccttt ttttaactgc agcatcgagc
     4381 aatcataata actctagctc gcaaatttat agtccgattg aaacctatac gcatagcctt
     4441 aaagatgtac caattgatat gaagtatgat agtgacaaat attttatcag tgggtattct
     4501 tatggtgcgg aagtttatct aacctccact aatcgtatca aattagattc tgaagtgaac
     4561 aatgatacac gtaattttaa gattgtggcg gatttaacac atagtcatcc aggtacagta
     4621 agtgtcaacc taagagtaga aaacctccct tctggggtga cagctaccgt atctccagat
     4681 aaaatatcag tcacaattgg caaaaaagaa tctaaggtat ttcctgttag aggaagcgtt
     4741 gatgctaaac aaattgcaaa tggttatgaa attagcaaaa ttgaaacggg ggttaataag
     4801 gttgaagtga ccagtgatga atctaccatt gctttaattg atcacgttgt ggcaaagtta
     4861 cctgatgatc aagtgttaga taggaattat agtagtcgag tgaccttaca agctgtctca
     4921 gctgatggaa ctattttagc aagtgcaatt gatcctgcaa aaacaaattt atcagttgct
     4981 gtaaaaaaaa taacgaaatc agtcccgatt agagttgagg cagtggggat gatggatgat
     5041 agcttatcag atattcaata caaattgtca aaacaaacag ctgttatctc aggtagtcga
     5101 gaggtcttag aagatattga tgaaattatt gcagaagtta acatttctga tgtcactaaa
     5161 aatactagta agaccgtgag tttgtcttct agtcaggtgt caattgagcc gtcagtagtg
     5221 accgttcagt tgacaaccac caaaaaataa tggataataa ctaggaaaat tgaatggagg
     5281 aagccattag gcttatataa attatgggaa aatattttgg aacagatggt gttcgtggcg
     5341 aagcaaatgt tgagctgaca cctgagttag cttttaaact gggccgattc ggtggttatg
     5401 ttttaagtca acatgagacc gaaagaccta aagtttttgt ggcgcgtgac acccgtattt
     5461 caggagaaat gttggagtca gccttgattg ctggtctact atctgttggt attgaagttt
     5521 ataaacttgg cgttttagcg acacctggcg tctcttattt agttagaact gaaaaagcaa
     5581 gtgcaggtgt gatgatttct gcaagtcata accctgcgct tgacaatggc atcaaattct
     5641 ttgggaatga tgggtttaag ttagctgatg accaagaatt ggaaattgaa gctctcttag
     5701 atgctccgga ggacacgctt cctcgcccga gtgcagaagg acttggaacc ttggtagatt
     5761 atccagaagg acttcgtaaa tatgaaaagt tcttggtaac aacaggtact gacttaagcg
     5821 gtatgacagt tgccttagac actgctaatg gagctgcttc agtgtcagca cgtgatgtct
     5881 ttttagattt aaatgcagaa attgctgtca ttggtgaaaa accaaatggt cttaatatta
     5941 atgacggtgt tggttcaacg catccagaac aacttcaaga gttagttaag gaaacaggag
     6001 cagatcttgg tttagccttt gatggcgata gcgaccgtct gattgctgtg gacgaaactg
     6061 gcgagattgt tgacggtgat cgtattatgt ttatcattgg taaatatttg tcagaaaaag
     6121 ggcttcttgc tcacaatacc attgtcacaa cggtgatgtc taacctcggt tttcataaag
     6181 ctttagataa acaaggtatt aataaagcaa ttacagcagt tggagaccgc tatgtggttg
     6241 aagaaatgcg tagctcagga tacaatctcg gtggtgaaca atcaggccat gtcattatca
     6301 tggactacaa taccactgga gatggtcagt tgacagctat ccaattagcc aaggtgatga
     6361 aggaaactgg gaaatcactt tcggaattag ctgctgaagt aaccatttac cctcaaaaac
     6421 tggtgaatat tcgtgtggaa aacagcatga aagaccgtgc tatggaagtt ccagctattg
     6481 cgaagatcat tgcaaaaatg gaggatgaaa tggctggtaa tggtcgtatt ctcgtgagac
     6541 caagtggtac agaacctctt cttcgtgtca tggcagaggc gccaacagat gccgaagttg
     6601 attattatgt cgatactatt gctgatgtcg tccgtacaga aatcggatgc gataactaac
     6661 gagttgagta caaacaagaa accttatttc taaggcttct gtcaacaatt ggaaagaggc
     6721 ttggggcgtt tagcccaagt ctctttttat aaattactaa agtagaagag aaaggatatc
     6781 ggatgacaaa acagcaaatc gaaaaactag cggacttact gcctcctttt gcagccacac
     6841 tttacttaga tatgattcct gaatttcaag aaggttatgc ttcttatgtt tatcaaacac
     6901 aaaaacaaga tactcaaaac cgtcgcatca agcagttaac taaaaatttt gaacgattac
     6961 tagatacccc atctttttat gtgatgcctg tgacaagaaa aacagcagag caattgctag
     7021 cagataaagg cctagcttat catttgttat cgacagttgc agctaatcaa gtgtttgcgg
     7081 ctgctgttaa cgaggatcag acgctttttc aagtgcttcg aaatggagca agagtagcct
     7141 ttgtgattct tcaagaaaag ggggaatttc tagaaatgac tttagggatt cttgaaacag
     7201 tgacagaaaa cgaggttcca gcagctattg tggaggctgt ccagacatat cttctggcag
     7261 atatgcaaga gaaactccct tctttaagca ttatggtcag tcaagaatca agcaagcttt
     7321 tttaccaaga acaagggttt gttttgcacg ctaagggttg caagccagga aacgaaaatg
     7381 attctgacgt ttgtcagctc ttaaaatact tttccaagcc atgaggaatc aagggaaatg
     7441 gtttattcat agaaacattt tatcatcaaa gtggtataat aagaccatgt caaaaaaacc
     7501 tacttcagcc tatgtgcaca ttcctttttg cacacaaatt tgttattatt gcgatttttc
     7561 aaaagtcttt atccaaaatc aacctgttga tgcttatttg aaagccttga ttcaagaatt
     7621 tgactcttat ggcattcgag acctaaaaac cctttatatt ggaggaggta cgcctacagc
     7681 tatcactgct aaacagttgg agtatctcct aaatcacctt gaacgaaacc tcaatttaga
     7741 tgatcttgaa gaattcacaa ttgaggcaaa tcctggtgat ttgacgccag aaaaaattgc
     7801 ggtgcttcag aggtcagctg tcaatcggat ttccttgggg gtgcagactt ttaataacaa
     7861 gcaattaaag cagattggcc gtagccataa tgaagaacaa atttatagca ccatcactaa
     7921 tttaaaagct gcaggctttc acaatatttc cattgattta atttatgcct tgccaggtca
     7981 aaccttagat caagtcaagg aaaatgttgc taaagctttg gcacttgata ttccccatct
     8041 tagtctttat agccttattt tggaacatca caccgttttt atgaacaaaa tgcggcgtgg
     8101 taaacttaac ttaccaacgg aagacttgga agcggaaatg tttgagtata ttatttcaga
     8161 aatggaagct aatggttttg agcattatga aatttccaat tttaccaaac caggttttga
     8221 aagtcgccat aacttgatgt attgggataa tgtggagtat tttggatgtg gtgcaggggc
     8281 ttcaggttac cttaatggca ttcgttatca aaatcgggtg cccatccagc attatcttaa
     8341 agcggttgaa gcaggaaacg ctagactaaa tgaagaagtg ctgcgcaaag aggaaatgat
     8401 ggaagaggaa ctctttttgg gcttacgtaa aaagacagga gtctccatcc agcggttcca
     8461 agaaaaattt ggtatgtctt ttgaggagcg ttatggaaat attgttaggg agcttcaaaa
     8521 ccaaggactg cttgtcaaag atgacgcttt tgtcagaatg actaaaaagg gtcttttctt
     8581 aggtgattct gttgctgagc ggtttatatt agattaggag agtattatgg gattaagtta
     8641 tcaggaggag ttgacacttc cttttgaatt atgtgatgtc aaatcagata taaaattgcc
     8701 ccttttatta gactattgtt tgatggtttc tggtagacag tctgcgcaat taggacgaag
     8761 taacaacaat cttttagtcg attacaagct tgtttggatt gtaacggatt atgagatcac
     8821 tattcatcgc ttgccacatt ttcaagaaac catcaccatt gaaacaaaag ccctctccta
     8881 caataaattt ttttgttatc gccaatttta tatttatgat caagaggggg atcttttagt
     8941 ggatatctta gcctattttg ctttgttaaa cccagatacg cgaaaagtgg caactattcc
     9001 agaagattta gtagcgcctt ttaagactga ttttgttaaa aagctacacc gtgttcctaa
     9061 aatgcctctt ttagaacaat caattgatcg tgattattat gtgcgttatt ttgatattga
     9121 tatgaatggt catgtcaaca acagtaaata tttagattgg atgtatgatg tgttggggtg
     9181 tgagttttta aaaacgcatc agcctcttaa gatgactttg aaatatgtta aagaagtctc
     9241 accaggcggt caaattactt ccagttacca tttggaccaa ttaacttctt accatcaaat
     9301 cacctcagat gggcagctga atgcccaagc catgattgaa tggcgagcga ttaaacaaac
     9361 agaaagcgag acagactagt gccttataag ggttatttaa ttgatttaga cggaacgatt
     9421 taccaaggga aaaaccgtat tccagcaggg gagcggttta tcaaacgcct acaagaaaga
     9481 ggcattccct atttattagt gactaataat accacaagaa cacccgaaat ggtgcaaagc
     9541 atgctggcta atcagtttca tgttgagaca agcattgaga ccatttatac ggctaccatg
     9601 gcaaccgtcg attacatgaa tgacatgaac cgtggcaaga cagcttacgt tattggagaa
     9661 acagggctca aatcagcgat tgcagcagca ggttacgtag aagaacttga gaatcccgct
     9721 tacgtggtgg ttggtttaga cagtcaggtg acctatgaga tgttggctat agccaccttg
     9781 gctattcaaa aaggtgccct ctttattggg acaaatcctg atttaaacat tccaaccgaa
     9841 cgtggtttga tgccaggagc aggtgcattg aatgccttac tagaagctgc cacacgtgta
     9901 aaacctgttt ttattggcaa gcccaacgct atcattatga ataaatcact agaggtattg
     9961 ggcattcagc gctctgaagc cgttatggtt ggcgataatt acttgactga tatcatggca
    10021 ggtattcaaa atgatattgc aacgatcctc gtgacaacag gctttaccag acctgaagag
    10081 gtaccaacct taccaattca gccagatcat gtcctatcta gtcttgatga gtggaggttg
    10141 tgatggtaga aaacactaaa ttactatgta gctggggatg gttattagca ttagctattc
    10201 tcataacgat ctattcaacg tggctgtggt atcctcttga ggtggaccat ctaaagttag
    10261 aacaagttgt ttttatgagc aaggatgcta tcttgcataa ctataacgga ctactaaatt
    10321 accttaccaa tccttttgta acaaggttgg aatttgctaa ttttcactcg tcagcagacg
    10381 gactcaagca ttttgcagat gtgaagtggc tttttcacct aactcaagtc gtttttcttg
    10441 gcctccttta cccaaccctt aagactttca cacaaaggtt aaaaaccaaa cgtttctggc
    10501 tccttcaaaa acctttaata ctagcagctt tgtttcctct tatgattggt ctaatggcta
    10561 gtttcattgg gtttgaacat ttttttactc tttttcatca ggtgcttttt gtaggagata
    10621 gcagctggct ctttgatccc ttgaaagatc ctgtgatttg gatccttcca gaagtctttt
    10681 tcttgcactg tttccttttc tttatgatag tttacgaaat catcctgtgg agcttagtag
    10741 gtttagcaag atggcagcgc ctaaatgtag aatgataaaa ggattaagag attaatttcc
    10801 ctaaaaatga taaaacaagc gttttgaaag cgcttgtttt ttagtttgca gtcagagtag
    10861 aatagaagta tcaaaaaaac accgactcgg tgccactttt tcaagttgat aacggactag
    10921 ccttatttta acttgctatg ctgttttgaa tggttccaac aagattattt tataactttt
    10981 ataaca
//
LOCUS       DAUGFP010000049        10450 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.049, whole genome shotgun sequence.
ACCESSION   DAUGFP010000049 DAUGFP010000000
VERSION     DAUGFP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.049"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            311..1855
                     /locus_tag="VH663_001390"
     CDS             311..1855
                     /locus_tag="VH663_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ8174585.1"
                     /translation="MSILEVKQLSYGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGYLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DFSKLT"
     gene            2219..3442
                     /locus_tag="VH663_001391"
     CDS             2219..3442
                     /locus_tag="VH663_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ8174586.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLILINQDITRTQAHQKKRLKKL
                     EDQKASLERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPKVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
     gene            complement(3484..5046)
                     /gene="guaA"
                     /locus_tag="VH663_001392"
     CDS             complement(3484..5046)
                     /gene="guaA"
                     /locus_tag="VH663_001392"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEQ8174587.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            5213..6646
                     /locus_tag="VH663_001393"
     CDS             5213..6646
                     /locus_tag="VH663_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174588.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKEPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            6729..7427
                     /locus_tag="VH663_001394"
     CDS             6729..7427
                     /locus_tag="VH663_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8174589.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            7516..7857
                     /locus_tag="VH663_001395"
     CDS             7516..7857
                     /locus_tag="VH663_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEQ8174590.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            7872..9434
                     /gene="ffh"
                     /locus_tag="VH663_001396"
     CDS             7872..9434
                     /gene="ffh"
                     /locus_tag="VH663_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEQ8174591.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQLFGKGFKGKIGQFTMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(9551..10249)
                     /locus_tag="VH663_001397"
     CDS             complement(9551..10249)
                     /locus_tag="VH663_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ8174592.1"
                     /translation="MFSGHQLKTARLSKGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
BASE COUNT         3477 a         1789 c         2018 g         3166 t
ORIGIN      
        1 ttagcttata taggtactca gctaccactt tcggtatctt tttgggtagc tcttggaaaa
       61 attggtggta gtttcggaac atcttttgtg actttagttg ctgttcctct tttgtatcct
      121 gttatcaaaa acttaaaaga aaaggttaca atcggacaag caaaaagagg tttttgatac
      181 aagtacctta tcgataaaaa agcaaggtac acttttagtc aaatattgac aaaaggttac
      241 aattttgcca taatgaaata actataaagc cgttttgacg gcttttttga tgacagttaa
      301 ggaagaaacg atgagtattt tagaagttaa gcagttgagt tacggttttg gggatcgggc
      361 tatttttgaa aatgtgtcat ttcgcctctt aaaaggcgaa catattggac tagttggggc
      421 aaatggtgaa ggaaaatcaa cctttatgag tatagtcaca ggacatttac agcctgatga
      481 aggaaaggta gagtggtcga agtatgtcac tgcaggttac ctggatcaac atacagtgtt
      541 ggaatcagga caaaccgttc gtgatgtctt gcgaactgct tttgatgagt tatttaagac
      601 cgagaatcgt attaatgaga tttacgcgtc aatggcagat gataaagctg atattgctgt
      661 tttgatggaa gaagtaggtg agcttcaaga ccgtttagaa agtcgtgatt tctatacttt
      721 ggatgctaag attgatgaag tagcgcgtgc gcttggtgtt atggattttg gaatggagtc
      781 agatgttaca tccttatcag gtgggcaacg aacaaaggtt ttattagcca aattactatt
      841 agaaaaacct gatattctgc tattagatga accaactaac catttggatg ctgagcatat
      901 tgaatggtta aaacgctatt tacaacatta tgaaaatgct tttgtgttga tttcgcatga
      961 tatttctttc ttaaatgatg tgattaatat tgtttaccat gttgaaaatc aaagtttagt
     1021 tcgctatact ggggattatt accaatttca agctgtttat gagatgaaac aatctcaact
     1081 tgaagcagcc tatgaacgtc aacaaaaaga gattgctaac ttgcaggatt ttgtcaaccg
     1141 aaataaggct cgggtagcga cacgtaacat ggcaatgtct cgccaaaaaa aacttgataa
     1201 gatggatata attgaacttc aagctgagaa accaaaacca aattttgaat ttaagcaagc
     1261 tagaactccc agtcgattca tttttcaaac aaaaaatctt gtgattggtt atgattaccc
     1321 attgaccaaa gaacccttaa atataacgtt tgaaagaaat caaaaaattg ctattgttgg
     1381 ggccaacggt attggaaaat ctactttgct aaaaagttta ttaggtgtta tcgagccttt
     1441 agaaggtcat attgtcacag gggatttttt agaagttggc tactttgaac aagaagtgac
     1501 aggtgttaac cgacaaactc cgctagaagt agtttgggat gcttttcctg ccttaaatca
     1561 ggcagaagtt cgagcggcac tagctcgttg cggactaaca tcaaaacata ttgaaagtca
     1621 aattcaagta ctttcgggtg gtgaacaagc aaaagttcgt ttttgtttgt tgatgaatcg
     1681 tgaaaataac gtgcttattt tagacgaacc aacaaatcat cttgatattg atgctaaaaa
     1741 tgagctcaaa cgtgctttaa aagcatataa gggttctatt ttaatggttt gtcatgaacc
     1801 tgatttctac aatgggtggg taaccgatac ttgggatttt agtaagttaa cctaaactat
     1861 tccttaattt tttacacagg aattactaaa atcagtaaag atatagaata aatactgcta
     1921 gatatgtgat ggtttaggta actgatcacc aaccaaaaaa gatgtgacta catctttttt
     1981 tatctaatgg aatggtgtaa agtcgtttaa taatagctta ttttttgatg atcagttgtt
     2041 catgaaattg ttggtgatta acaagtcatt taagatacaa acgttattac taaaaagtga
     2101 ttcaaatagt ttccctattt ttatgttttt ttaaaattta ttacgaataa atagataaag
     2161 acagtgaaga tgattagtaa gtacatctgc taagattacg aatagatggg gacaataaat
     2221 gacgaattta acgttttatg caaaaatcgg tatttcagaa gaagagcatg actcttttgt
     2281 taaagaacat cagcaaatta gcgttttaca aggtagtgat tgggcaaaaa tcaaaaatca
     2341 atggcagaat gagcgaattg gtatctataa agaggaaaag caggttgcct ctttatcact
     2401 tttgattaag ctattgccac ttggaagaag cattatttat attccaagag ggccagtcat
     2461 ggattatctt gaccgtgatt tggtggcatt taccatgaaa acactaaagg attatggtaa
     2521 aactaaaaag gccctcttta tcaaatatga tccagctatc ctgttaaaac aatacgcact
     2581 gggacaggaa gaagaagaaa aacctttagc tttagcagct attaagaatc tccaagaagc
     2641 tggtgttcat tggactggtt taacaatgga gattgcagat agtatccaac ctcgtttcca
     2701 agctaatatt tacactcaag aaaaccttga gatgcaattt cctaagcata ccagacgttt
     2761 aataaaagat gctaagcagc gtggtgtaga aacatatcgt gccagtcaat cagaacttca
     2821 caaattttcc aagattgtct ccttaacaga aaaacgtaaa aatatttctt tgcgtaacga
     2881 agcttacttt caaaagttga tgactactta tggggataag gcctacttac atctagcaaa
     2941 agtgaatatt cctcaaaaac tagatcaata ccgccagcaa ttaattctta ttaaccaaga
     3001 tattactcgc acccaagctc atcaaaagaa gcgtttaaaa aaattagaag atcaaaaagc
     3061 ttctttagaa cgttatataa ctgaatttga aggctttaca gaccaatatc ctgaggaagt
     3121 tgttgtagca ggtatattat ctatttctta tggaaatgtt atggaaatgc tttatgctgg
     3181 gatgaatgat gattttaaga agttttatcc tcagtatctg ctgtatccta aagtttttca
     3241 ggatgcttat caagatggta ttatttgggc taacatggga ggagtagaag gctcgcttga
     3301 tgatggactt accaaattta aggccaactt tgctccgaca atagaagaat ttataggaga
     3361 atttaatctc cctgtcagcc cactttatca tattgctaat accatgtaca aaatacgaaa
     3421 acagttaaag aataaacatt aaaagactcg ccaaaatgag cgagtctttt aatgtttatt
     3481 ctgttattcc cattcaactg ttgcgggtgg tttacttgtg atgtcgtaga cgatacggtt
     3541 aacgtggtca acttcattta cgatacgtgt tgagattttt ttcaagacat cccaaggaag
     3601 ttgagcaaag tcagctgtca taccatcaat agacgtaata gcacgaatgg cgatggtata
     3661 atcataagta cgaccatctc ccatgacgcc tacagaacgg acacctgtgt taactgtaaa
     3721 gtattgccac acgtcacgat caagtccagc cttagcaatt tcttcacgaa ggatagcgtc
     3781 tgattcgcga acggtttcaa gtttttcttc agtaattgct cccatgacac ggatagcaag
     3841 tccaggacct ggaaatggtt ggcgccaaac aatttcttca ggcattccaa gagcgattcc
     3901 aagcgctcga acttcatctt tgaaaagagt gtttaagggc tcaatcaatt caaactgcat
     3961 gtcttcgggg agaccaccca cattgtgatg tgatttgatg gtttgagcag tttctgttcc
     4021 tgactcaatg atatcagtat aaagtgttcc ttgggcaagg aagtcaacac cttttaattt
     4081 gctggcttca tcatcaaaaa catagacaaa ttcattacca ataattttac gttttttctc
     4141 aggatcttca acgtctgcaa gaaggtctaa gaaacgtttt gaagcatcca cacggataat
     4201 atttaggcca aatttgcccc caagcattcc cataacttga tcgccctcgt ctttacgaag
     4261 aagaccgtga tcaacgaaaa tacaagttaa ttggtcaccg atagcttttt gaagtagaac
     4321 accaacaact gaagaatcaa ctccaccaga aagacctaga agaactttac gatcgcctac
     4381 agtttcacga attttagcaa tttccatgtc aataaaatta tccattgacc aatcaccacg
     4441 cgcgccacaa attgatatag caaagttttt aagaatgtca tttccataaa cagagtgtct
     4501 cacttctggg tggaactgaa taccgtaaag gtttttctca gtattttcaa tagctgcata
     4561 gggacagtca tttgagtctc caacaaggtg gaaaccttct ggaatttcag taacagcatc
     4621 accatggctc atcaaaacga gttgttcttg aggtgtgcct gaaaataatt ttgacgtttc
     4681 acgaagatga agggttgact gaccgtattc acgattacca gcttgtccag caggaacaac
     4741 tttaccacct aatttatggg tgattaattg cataccgtaa cagataccaa gaatcggaat
     4801 ccctagttca aagatttcag ggtcaatgcc aaaggcgtta tcagcgtaaa cagagttagg
     4861 ccctcctgat aaaacgatac ctatgggatt gatctcacga agttcttgag cggtgatttt
     4921 atggcttttt agttcggaga aaacaccaaa ctctcgaata cgtctagcaa taagctgatt
     4981 gtactggcta ccataatcaa gaacgataat tttttgaaca tcattcaaaa ttgaaatttc
     5041 agtcattata atcctttcat tctataccaa ccgtcaggct gatagttgtc aacttattgt
     5101 agcatatttt catcgaaaag acattacaat ttctctagct cgttggaaaa aaacgtaatc
     5161 gttatgttac aatagaggct atgaaaggga attaagacaa cagggagggc ctatgctaag
     5221 aaaaaagaga attttgggtt tatttcgacc tgttgaatta attttcctag gattattgtt
     5281 gagtttagtt gtttcgtacc ttgcgtggac caatagtttt gcaacacttc acaatatatt
     5341 agcaactgtc ggaatagtgg aacgaagcaa agatcaacag cctcgctatc acattggaca
     5401 agctattcaa gtacaaaaaa gtggtccata tcatcaatgg attggaacta ttaataaaca
     5461 ggtagaagat atagccgaaa attaccgagt gagttatcat tatgaagtgg tatttccaat
     5521 aggaaaagtc actgtttctt tgccagaaca taacctgaaa gagcctgata aaccgaggtt
     5581 taaaaaagga gatatcgtta aattatcttc attaactaaa aagccacata taaaagtgta
     5641 tcaaggtcaa ttagcgacta ttaaacaagt taaaaaacgc tatgactatt cgttaggagg
     5701 atatcagtac gatattaacc tgaaagataa tctaagatta gatggaattt cagagcaaga
     5761 ttttgttaaa ccttattata ttaggttcaa taaaggaaat tcccctgagc aaaacaatcg
     5821 tcttttgcga aaagctttcg cttatgcaaa gcagcatcca aatagcgtta tatcttttcc
     5881 aaaggggcaa tttcacattg gctctttgcc ttcacaaaaa gattattttg agcttccatc
     5941 tgatacagct attattggtc atcagacaga gttcattatt cacggtaaaa tgttgtggtt
     6001 tggattccct acaggaccaa aggctgaaca aggtgttcgt aatctcgtgt tgactggagt
     6061 gcatttcaaa gcaaatgatt tgaaaaaagg agaccacttt atgattatgg ctgatcatgg
     6121 tactgattgg catatttacg ataacaaatt tactatggtt cataagcgta atagtcatat
     6181 ttttgattta ggatctctac aaaattcatt gtttgagaaa aaccaattta ttggctatgc
     6241 gccagaatta gtacaagacc aacagctgct atcaaaggct caagggcatg attttttttc
     6301 agaagtcatt cagtttgatg ctgctgttca tcattttgca tgggatggag gtctacttag
     6361 taatattgct ccaaactatg aagcatttaa ccaaactcga catctatgtc acaatattac
     6421 tgtaagccaa aatcaatttt taccttatat agatccgact ggttgcctga gagcctatag
     6481 tggttctatt ggtcagcatt cctcaaaggt aggagttatt agggttttaa ataatgtttt
     6541 tacctcatcc attgttacta aagcgaagct cactagttgg tttatggaac ctattcattt
     6601 tccaccaaat tcaccagtta ttgtcgcagg taatatcatt aattgatgcc aaatacctca
     6661 atatttacaa ttcaattctc atcaaaaata tgataaaata gatgaataac cgacttgagg
     6721 agtgtggcat gttaccagct tatataaaaa ttcatgatgc aattaaaaaa gacattgatc
     6781 tgggcatctg gccaattggt agtcgcttac caagtgaacg acatttagcg gaacatttca
     6841 ccgttagtcg aatgactcta cggcaggcta ttacattact agtagaagaa gggatccttg
     6901 aaaggcggat aggaagtggt acttatgttg ccagtcatcg tgttcaagaa aaaatgcgag
     6961 gaacaacaag tttcacagaa attatacgct cccaaggacg acaaccctcc tctaaattat
     7021 tatcctatca aaagcaatta gctagtgata ccgaagttaa agaattgaac ttagacaaaa
     7081 cagacttggt cattcgaatg gaacgtatcc gctatgcaga cagtgtccct ttggtgtacg
     7141 aaattgcttc aatcccagaa aaatttataa aaacggttaa acgagctgat attacagaac
     7201 acttttttca ctcattgata gcaaatggtt acgagattgg taaaagtaaa cagacaatct
     7261 acgctaagct agcaagtgag cgtgtagcct cctacttgga agttgctaaa ggacatgcca
     7321 ttttggctct aactcaggtg tcttacttta cagacggcaa accttttgag tatgtccgca
     7381 gtcagtatat cggtgaccgt tttgaatttt acttagaaaa taattagtaa agaagtcatt
     7441 tatgacttcc ttttttacac gaacagcata aggcaattaa tttgctactg ttaaaaacaa
     7501 tcatagtatg ataaaatgaa cattatggaa attgaaaaaa caaacaggat gaatgccctt
     7561 tttgaatttt atgcagcttt gctaacagat aaacaaatga attatattga actgtattat
     7621 gccgatgatt atagcttagc tgagattgct gatgaatttg gtgtcagtcg tcaggctgtc
     7681 tatgataata ttaagcgtac ggaaaaaatt ttagagactt atgagatgaa acttcatatg
     7741 tattcggatt atgttgttcg aagtgaaatc tttgacgaca tgattgctca ctaccctcat
     7801 gatgagtatt tgcaagaaaa aatttccatt ctaaccagta ttgacaatag agagtaagag
     7861 gagttttgac tatggctttt gaaagcttaa cccaacgttt gcaagatgtg tttaaacaca
     7921 ttcgcggtaa aaagaaatta tcagaatcag atgttcaaga agtgacaaaa gagattcgtc
     7981 tagctctttt agaagccgat gtcgctttac cagttgttaa aacctttatt aagcgcgtac
     8041 gtgagcgcgc tattggccat gagattattg atactcttga tccaacacaa caaattctta
     8101 agattgttaa tgaagaatta acgagtattt taggctcaga aactgcagag attgataaat
     8161 ctcccaaaat tccaactatt atcatgatgg tcggtttgca aggggctggt aaaacaactt
     8221 ttgcaggaaa actcgctaat aaattaatta aagaagaaaa cgcgcgacca ctgatgattg
     8281 ctgccgatat ttaccgtcca gcggccattg atcagttaaa aacactgggg caacaaatca
     8341 atgtacctgt ttttgatatg gggacagacc actcagcagt cgatattgtc agaaaaggtt
     8401 tggaacaagc tcgtgaaaac cgcaatgact atgtcttgat tgatactgcg ggtcgcttgc
     8461 aaattgatga aaaactgatg gaagagctgc gcgatgtcaa ggctttagca caaccaaatg
     8521 aaattctttt ggttgtagat agcatgattg gtcaagaagc tgccaatgta gcctacgagt
     8581 ttaatcatca gttgagcatc acaggggttg tgttaacaaa gattgatggt gatacacgtg
     8641 gaggtgcggc gctatctgtc cgtgaaatca ctggaaaacc aattaaattt acaggtattg
     8701 gtgaaaaaat cacagatatt gaaacatttc acccagaccg catgtccagt cggattttgg
     8761 gaatggggga tttgcttacc ctcattgaaa aagctagcca ggaatatgat gagaaaaaat
     8821 ccttagagtt agctgaaaaa atgcgtgaaa acacctttga tttcaatgat tttattgaac
     8881 agttagatca agtgcaaaat atggggccta tggaagacct cttgaaaatg attccaggaa
     8941 tggctggtaa cccagctttg gccaatatca aagttgacga aaaccaaatt gctcgcaaac
     9001 gtgctattgt ctcttcaatg acacccgctg aacgtgaaaa tcctgactta ctaaacccga
     9061 gtcgtcgccg tcgtattgca gctggttccg ggaacagctt tgtagaagtt aataaattta
     9121 tcaaagactt taaccaagcc aaaagcatga tgcaaggggt tatgtcgggc gatatgtcta
     9181 aaatgatgaa agatatgggt attaatccca ataatcttcc taaaaacatg cctgctggta
     9241 tgccagatat gtcttcctta gaaggaatga tgggacaagg tggtatgcct gatttatctg
     9301 gcctcggtgg tgatatggat atgagtcaac tatttggtaa agggtttaaa ggaaaaattg
     9361 gccaatttac catgaaacag gccatgaaac ggcaggctaa caaactaaaa aaagcaaaga
     9421 aaaaacgaaa ataaaagaga cgaaaatttt cgtctctttt tgatgtgaaa ttcatttatc
     9481 ttgtatacaa gattattaaa attacggaag accgtgttac ataccatgat gagttcatta
     9541 tcaatctaac ttaaatttct aagggcctaa aattagcgat aattttgcca ataatgcgag
     9601 gttcttcgct atagggagca aacttatcag aatatttttt atttaaggac actagacgta
     9661 atccctcatc ttcacgaaat acctttttga tatatgtttg cccatcccat tcgactgcat
     9721 aaattgctcc atcgtaatca aaactatttt gttttataag aacaacttca ccatttaaat
     9781 aagttggctc catagaatct ccaaaaaccc aagacgcaaa atcgtattct aattgttcat
     9841 cgtaaaagac aacatcaaag ttaccatcac caaaatagga gtaaccagta ccagcagata
     9901 aactttcata gacccgataa gcatataatt gtgatggttt atctatgaga tctttagatt
     9961 ttttgtctat ttgatgattt aacaatcgct ctgaatagcc aattactttt tctttattag
    10021 atattgtcag ctggttataa ggcgttagca atctatagtt tgggtcgaaa taatcagatg
    10081 tcacattgaa tagatgcaat aaggcattta aatgtttttc atttggtata ttcttacctt
    10141 tttcccaatt agatattgtc attttattga catgcaacaa tcttcccaat tctgattgag
    10201 ttattccctt tgataatcgt gctgttttta gttgatgtcc agaaaacatt tggtagctcc
    10261 tttagtaaag ttataacttt actaatagta tcaaaaaaat aaaacgaaag caagtgtaac
    10321 attttctaca attaattcat gtgatagtaa cactgtcaaa aagggggaac acctaaaaca
    10381 taacatagcg actagattcg tgtactagca ggtctatatc tgataaagag ttaattactt
    10441 taaagataaa
//
LOCUS       DAUGFP010000050         9931 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.050, whole genome shotgun sequence.
ACCESSION   DAUGFP010000050 DAUGFP010000000
VERSION     DAUGFP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.050"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            35..2047
                     /locus_tag="VH663_001398"
                     /pseudo
     CDS             35..2047
                     /locus_tag="VH663_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888982.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
     gene            complement(2129..2539)
                     /locus_tag="VH663_001399"
     CDS             complement(2129..2539)
                     /locus_tag="VH663_001399"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ8174593.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLNLTGLTAQICQHELDHLEGILI"
     gene            3254..3331
                     /locus_tag="VH663_001400"
                     /pseudo
     CDS             3254..3331
                     /locus_tag="VH663_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3429..5306)
                     /locus_tag="VH663_001401"
     CDS             complement(3429..5306)
                     /locus_tag="VH663_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ8174594.1"
                     /translation="MSHFLVEKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRIGFDGDHSPFSKANDYKIAYLTQDPEFNDASSVLDTVLSSDVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDATNLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKISVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRIFYGNYSDYLDEKAFEKETVEADLAKAIVTEEMPLPPKEEKKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(5303..6511)
                     /locus_tag="VH663_001402"
     CDS             complement(5303..6511)
                     /locus_tag="VH663_001402"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEQ8174595.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDH
                     IEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            complement(6636..7484)
                     /locus_tag="VH663_001403"
     CDS             complement(6636..7484)
                     /locus_tag="VH663_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ8174596.1"
                     /translation="MKLAVITDSTATLPIDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDRTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            complement(7484..7864)
                     /locus_tag="VH663_001404"
     CDS             complement(7484..7864)
                     /locus_tag="VH663_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEQ8174597.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            complement(8110..8267)
                     /locus_tag="VH663_001405"
                     /pseudo
     CDS             complement(8110..8267)
                     /locus_tag="VH663_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            8766..9791
                     /locus_tag="VH663_001406"
     CDS             8766..9791
                     /locus_tag="VH663_001406"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HEQ8174598.1"
                     /translation="MRKRCYSTSAAVLAAVTLFALSVDRGVIADSFSANQEIRYSEVT
                     PYHVTSVWTKGVTPTANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNM
                     LHWWLDQNNEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKA
                     FPGLSARRIGVMPDLVLDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGD
                     QSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSN
                     GNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQ
                     DSWNQTN"
     gene            9844..9931
                     /locus_tag="VH663_001407"
                     /pseudo
     CDS             9844..9931
                     /locus_tag="VH663_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
BASE COUNT         3173 a         1979 c         1623 g         3156 t
ORIGIN      
        1 ctataacgat tacgttaact tattaggaga ttttatgaaa aaatatttta ttttaaaaag
       61 tagtgtattg agtatcctga ctagttttac tctattagtt acagacgttc aagcagatca
      121 agttgatgtg taattccttg gcgtcaatga ttttcacggc gctcttgata ataccggaac
      181 agcttacaca ccaagtggta aaataccaaa tgctgggacg gctgctcaat taggtgctta
      241 tatggatgac gctgagatag atttcaagca agcaaatcaa gacggaacaa gtatacgtgt
      301 tcaagctgga gatatggtcg gagccagtcc tgctaactct gcacttttac aagatgagcc
      361 tactgtgaaa gtctttaaca aaatgaaatt tgaatatggc actcttggta atcatgaatt
      421 tgacgaagga ctagatgaat ttaaccgtat catgacaggt caagcgcctg atcctgaatc
      481 aacaattaat gatatcacca aacaatatga gcacgaagct tcgcatcaaa ccatcgtcat
      541 cgctaatgtc attgataaaa aaaccaatga tatcccctat ggttggaaac cttatgctat
      601 aaaagacata gccattaatg acaaaatcgt taagattggc ttcattggtg ttgtgactac
      661 agagattcca aatctcgttt taaagcaaaa ctatgaacac tatcaatttt tagatgtagc
      721 tgaaaccatt gccaaatatg ctaaagaact gcaagaacaa catgttcatg ctattgtggt
      781 tttagctcat attcctgcaa caagtaaaga tggtgttgtt gatcatgaaa tggctacggt
      841 tatggaaaaa gtgaaccaaa tctatcccga acatagtatt gatattattt ttgcaggaca
      901 taatcatcaa tacactaatg gaactatcgg taaaacacgt atcgttcaag ccctctctca
      961 aggaaaagct tatgcagatg tccgtggtac gctagatact gataccaatg attttattaa
     1021 aactccatca gcaaatgttg ttgctgtagc accaggtatc aaaacagaaa attcagatat
     1081 caaagctata ataaatcatg ctaatgatat tgttaaaaca gttactgaac gaaaaatcgg
     1141 aactgcaact aattcttcaa ctatttctaa aacagaaaat attgataaag aatcccctgt
     1201 cggtaactta gtaacaacgg ctcagcttac tattgctaag aaaacttttc caactgttga
     1261 ctttgctatg accaataatg gtggtattcg aagtgaccta gttgtcaaaa attaccggac
     1321 catcacctgg ggagctgcac aggctgtaca accatttggt aatatccttc aagtcattaa
     1381 aatgactggt caacacattt acgatgtcct aaatcagcaa tacgatgaaa accagaccta
     1441 ttttcttcaa atgtcaggtt taacatacac ttatacagat aatgatccta agaactctga
     1501 tacccccttc aagatagtta aggtttataa agacaatggt gaagaaatta acttaacaac
     1561 tacttacacc gttgttgtca acgactttct ttacggtggc ggtgatggct tttcagcatt
     1621 taaaaaagct aaattagtcg gagctattaa cacagatact gaagctttca tcacatatat
     1681 cacaaattta caagcatcag gtaaaactgt taatgctact atcaaagggg ttaaaaatta
     1741 tgtaacttca aaccttgaaa gatcaacaaa aattaatagt gctggcaaac acagtatcat
     1801 tagtaaggtt tttagaaatc gtgatggcaa tatagtgtct agtgaagtca tttcagacct
     1861 tttgacttct actgaaaaca ctaataacag ctttggcaaa aaagagacaa caacaaacaa
     1921 aaatactatc tctaattcca ctcttccaat aacaggggac aattataaaa tgtctcctat
     1981 tatgacaatc cttgccttga taagcttagg tgggctaaac gctttcatta aaaaaagaaa
     2041 atcctagcgt ttactagtaa ttgatcatcc ttatataatc aggcattttc gccaaaaaaa
     2101 cctaaagtgt cctactctag gttttttgtt aaattaaaat accttctaga tgatctaatt
     2161 cgtgctggca tatttgagca gttaggcctg ttaagtttaa acgctttggt cgccaattat
     2221 ggtccaaata ctcaacagta atcttatcat accgctgtgt tttgcgatag ccagataatg
     2281 acaaacaaga ttctttagtt tggtaaattc cctttttaga aaccatcaca ggattaaaca
     2341 tcaccaaatc aataaatccc atactcacaa taacaatgcg cttttgttca ccaatcatat
     2401 tggcagccat cccaagacat tcctgtctat aaaaagctaa ggtatcttgt aaatcttggc
     2461 caatccatag atcttctttc ttggcgactt gcgctttttg ctgtaataaa aaatgatctg
     2521 ttatgatttc tctaatcatt cttatctcct taaaccttct ttttaattca taaactacat
     2581 gttcaatgga ctattatcac catatgatag acttagtatc atctgtttaa aacatcaggt
     2641 gattccttat ttcaagatat cttgggcaac tttatgaaat tctgcaatat gagtaccaaa
     2701 taaaaattga cctgcatcag cagattgttg ctaagttctc atcacaatat tatcgaattt
     2761 actgtctgct tcttcaaatg tctaagagag atagttattg ctttgtgaga ttttcaaacc
     2821 agatactgct ttctcttgca tttacagcct tgataggtcc aaacaaaaca cctgctgttt
     2881 gaaatgcttt aatagctgat atgttgttaa tagttagtat tataaatttt ctgaatattt
     2941 ctgtcaagtt tttcagttga cttaactttt ttacttgatt tttcctcacg taataataga
     3001 aaagaagcgc cataatgtaa actacacccc aaacgttaga taaaaaatct aacgtttggg
     3061 gtgtttttgt atgacattaa gctatgaaga taagctagac atttagcgct taaaaactga
     3121 gggaactccg tttgaaagaa gcatctctgc aaagaagaag acaaaaaacc attataaaga
     3181 atggacaaag acgagttctg attagatttt cttttaagaa cggctaaatt gactcgttct
     3241 acctactatt atcatcgatt attgcaacaa taaacgtatc aaaataaaac gaaaagaact
     3301 tagtcctcta caatacagaa ctaaatcctt tacttcaaat agagtagcca acttttgggg
     3361 ctcagtgcaa gagcacttct tttctattat cacttgttta ttataaacat aacattacca
     3421 tagtatactt atatgtctag tccactcaaa tactcaaacc gttcatacgc caataataaa
     3481 tcattattgc gctgatcgag ttccttttgt aattgagcta actggccata atcactaact
     3541 acagttaaca tttggttttc aatttcttca atattagcct caatagttgc tattttatct
     3601 tctatctgag cccattcttg tttttcaagg taggacatgc gctttttctc ctctttcgga
     3661 ggaagtggca tttcttcagt gactattgct ttagctaagt cagcctcaac tgtttctttt
     3721 tcaaaagctt tttcatcaag gtaatcactg taattcccat aaaaaatacg aatatcgcct
     3781 tcttcaaagg ctaaaatctt agtggctact ttgtctagga aataacgatc gtgactaacc
     3841 gtaatgactg gtcctgcaaa gttagctaaa aagttttcta agaccgtcaa ggtggcaatg
     3901 tctaaatcat ttgttggctc atcaaggaga agaacatttg gcttttcaat gagcagtttt
     3961 agcaagtaga gccgtttttt ctccccacca gataatttag caatcagcgt gccatgactt
     4021 gatctcggaa aaagaaattg ctctaaaagc tcactgatac tcgttgttcc cacgcttatt
     4081 ttaacctcat cagccacttc ttgaaggtaa ttaatgaccc gtttggtctc atccatatct
     4141 ttgagctgct gagagaaata acctacacga atagtatctc caatatcaag cttcccagaa
     4201 gttggtttga gatcaccgtt gatgatattt aacagggtag attttccgac gccgttatct
     4261 ccaacaattc caatacgatc tttattttga ataatcaagt taaaatcctt aatcaactgt
     4321 cggtcgccat aagcaaaaga aaggtcttca aagtgaatca ccttcttccc aattcggctc
     4381 gtttcgaaag ccatctccaa tttatctgca gaactgtctt ggtgaacttc ttttttcaag
     4441 tcagtgaaac gattgattct agcctgctgt ttggttgcac gcgcctgtgg ctgcgtcctc
     4501 atccaagcta attcttgttt atagagttgc ttcttcttgt gtaaattagt agcatctcgt
     4561 tcgtcttgtt ctgcttttaa gcgcacatag tcttggtaat tgccttgata ttctgtcagt
     4621 cccgctttat ccaattcaaa aatacgggta gccacatgat caaggaaata acggtcatgg
     4681 gtaataaaaa ggacggtttt tttagctgtc ttgagatagg ttgttaacca agcgatggtg
     4741 tcaatatcga gatggttggt cggctcatcc aacaagagta agtcagcagc tcctagcaat
     4801 acttgagcta actggacacg tcttctcata ccacctgata ggtctcccac tttttgttcc
     4861 aaatccgtga tgcctaattt tgataggact gttttgacat cactttcaat agaccaagca
     4921 tctaagcgat ccatttcaga catgagggac tctaaggtct cttgtttatc ttcagtataa
     4981 ttcgccatca atagttcata ctgacgaatc agctgaatcg ccttaacatc agatgacaat
     5041 accgtatcta aaacagagga tgcatcgtta aattcaggat cttgtgttaa gtaagcgatt
     5101 ttgtaatcat tggctttaga aaatggtgaa tggtctccgt caaaaccaat ccttcccgac
     5161 aagacatcta aaagagttgt ttttcctgtt ccattaaccc caataatccc aatacggtca
     5221 aagtcatgaa taataaagga aatatcctga aagactgttt tatctcccac cgtttttgtc
     5281 aatttctcca ccaaaaaatg gctcatgttg ctaattcctc tctaacaaat gcttcaattg
     5341 tagtgaaatc attatctagg cgtccctcta caatcgctaa ttcaactttc tcaagcataa
     5401 gccctagctg aggacctggt ttcatcccaa aatccttaat caggtgagaa ccattaagga
     5461 cgatatcgta tttgttatga atggctagcg cttgatctaa agcttcaatg tggtccatgt
     5521 caacagataa gctttgagct tttcggaggc cttccactag acttgccatt tccttgccgt
     5581 actggtaaat atccaatttt tcaaaagatc tttcctgacg aattctataa agagcaatca
     5641 actttgtgac ataacgctga aaatcatttg aagtcttcca tgcttttagg aagcttttag
     5701 gatcctcaat ggctaaacta atcatcacat aagcccaagc ttggtggtga tcactaaagg
     5761 taaagttatc tttcaaagaa actatcagat gattcaagcc tgcttcctgc tgcttcaagc
     5821 ctggtaaata gtcataagcc tgacaagcaa tcatggcaga aatccctttt cgccagtgcg
     5881 gtgccatcaa taatttatca aattcagtaa aagaacgttc cacagaaatc ttttccaata
     5941 gaggtgaatg gctcctcata gcttcaaaag ttgcagcttc aatgtcaaaa tccagactcg
     6001 ccgcaaatcg aaagcctctc ataatgcgta gtgcatcttc ttcaaaacgt tcctctgctt
     6061 tacccactgc tcgtaaccgt ttttgctcca aatcaatgag tcctctaaat ttatcaataa
     6121 cttgaccatt ttcatccaag gccaaggcat tgacggtaaa gtctcttcgt tttaagtctt
     6181 cctctaaaga gcgcacaaaa gaaacctgac taggacggcg atagtcaaca tagacatctt
     6241 ctgtcctaaa ggttgtgatt tcatactcac cgccattttc aagcacgagc actgtaccat
     6301 gttcaatgcc aacatcgaca gttctgttaa aaatcgcttt agtctcttca ggataggaac
     6361 tagttgcaat atctacatca tgaatgggac gctcgagtaa aacatctctg acgcttcctc
     6421 ccacaaagta ggcttcataa ccagcttctt ttattttagt caatatgggt aaagccttct
     6481 gaaactcaga aggcattgtc attaatttca tacgttgttt tctaacctat catccaacta
     6541 gcccaatcta aagtgtgatt aggctacttg ggttctatca cattttgtta ttgttttagt
     6601 gattcttgca attaactatt ttttctaatc gacaattaaa gcctcggtgt tacgccaaat
     6661 gcaatggctc cttctcctaa atgagtagca atcaccgctc caaaatgaac ctcttcaatg
     6721 tcatattgat aaccactgtc ttgtaagagt cgtttcaaat aatcagcttt gtcttgtgct
     6781 tttgagtgaa taatagacac attatactga ccgtctgcta tcaagtcatt taaaatctca
     6841 accaagcgtt tcatggcttt cttttctgtt ctcacttttt cataaacaac aatttttccc
     6901 tcttcatcaa agcgtaaaat aggtttaata ctcaaaagat ttccaagaag agcagaccca
     6961 tttgacaggc gtccgccttt aaccagatga ttaaggtcat caaccataat aaaggctgtc
     7021 gtcctatcaa tctgttcttg cagcttgttt aaaatagctt gaaatgtcat tccttgacgt
     7081 gaccaatcta gcacattttt taccatacta cctaaaggag cagatgtgat cttactgtct
     7141 ggaaatgcca tttcaatctc aggatgttct tcagctaaaa attgaatatt ttgccaaaag
     7201 cctgagattc ctccagctaa gaacaagcca ataacatgtg tataaccctt agaagacaaa
     7261 agtcctaaaa gattatctag ttcagataaa cttggctgac ttgtcttggg aagattttgg
     7321 gagtcagcca tcttttgata aaaatcatct atactcaaat ttcgaccttc aaaataggtc
     7381 tcatcgtcaa tgataactgg tatatcaagg ctaaaaatag ctttatcttg tttcagatct
     7441 attggtagtg ttgctgtact gtcagtaatt actgctaatt tcattaattt ttaaactcca
     7501 aatgaatacc tggacgatcc aaggcaattt ctgtgacttc ataagtcaat cgcttaacca
     7561 tgtctagcaa aggagcagct aaggattcaa cttcctcttg cgtaaattcg cttggtttat
     7621 ccactaaacg atctaaaatg cgaaccattt gtgagatgac cccactaata acaaattctt
     7681 ctttgacaat aacaaattgc aatacagata caattactgt ttctttttgc tgctcatctt
     7741 tatcaatcaa ctgaaaagtc acttcaaaat tggtctctgg tgttccgttt tctttttccc
     7801 actctaaatt tctagcatca tagtggtatt gattgacaaa ttctttttca cgaactaatt
     7861 gcatttttta ttctccttgg tatcaatatg atccattata gcatagattt gaagattatt
     7921 aaagtagccc ctaaactact attttcgcat aacgttttaa acagtgctat ctctcgaaca
     7981 gatatctatt ttttcaccta tttatattta gtcgtcctta tactgacatt atatcaaaaa
     8041 acacccgtta gtggtcttgt actgcacctc aattaaaaca gaaccactgg tcaggtaagt
     8101 tttaagtcaa ggataaaaag gtttatcatt aaggcctcat catgcttagc caagtatttc
     8161 gaactagctt gtcttagctc tagtcgctca aacgtaaatc ctaggcgttc aatccgccgc
     8221 aaaacctctc caactaaccc atacttaaac ccatcgggct taatcatcgc tccgcccttc
     8281 ttcttttatc cattataaaa taaaaacgtt ttaacagaca aaaaatagga aagtaccaga
     8341 ctattggatt tattttttta tctcaaactc cattaacgat gttgagttaa aggcattcaa
     8401 aacaacatag atcgtaagct aacaagattt tttatcaatt gaacctatcg tccccagtta
     8461 taccacgacc tcttctggat gaggtctttt tttatttgtc taatttcatc aacgcttctt
     8521 cccttctttc ttctatcaca gctctcctat tgctcacttc ctcatgcagc aacatatcat
     8581 caaaaaaccg ttcattttta agcacaacca ctactgtatc tcttgtgatg tctccattgc
     8641 aatcgcttgt ttaattttat taaaaattat ttaaaaaatt aaatattatt ttaagaactt
     8701 gcaataatat atgtctatat tatataattt ctttatcata aaattatata gaaaaaggta
     8761 ttcttatgag aaaaagatgc tattcaactt cagctgcagt attggcagca gtgactttat
     8821 ttgctctatc ggtagatcgt ggtgttatag cagatagttt ttctgctaat caagagatta
     8881 gatattcgga agtaacacct tatcacgtta cttctgtttg gaccaaagga gttactccta
     8941 cagcaaactt cactcaaggc gaagatgttt ttcacgctcc ttatgttgct aaccaaggat
     9001 ggtatgatat taccaaaaca ttcaatggaa aagacgatct tctttgcggg gctgccacag
     9061 cagggaatat gcttcactgg tggttagatc aaaacaacga aaaaattgaa gcatatctaa
     9121 aaaaacaccc agataaacaa aaaatcatgt ttggtgatca agaattattg gatgtaagaa
     9181 aagttattaa tactaaaggt gaccaaacaa atagcgagct ttttaattat ttccgagata
     9241 aagctttccc cggtttgtca gcacgccgaa ttggagttat gcctgatctt gttttagata
     9301 tgtttatcaa tggttattac ttaaatgttt ataagacaca gactactgat gtcaatagaa
     9361 cctatcaaga gaaagatcgc cgaggtggta tttttgacgc cgtatttaca agaggtgatc
     9421 aaagtaagct attgacaagt cgtcatgatt ttaaagaaaa aaatctcaaa gaaatcagtg
     9481 atctcattaa gaaagagtta accgaaggca aggctctagg cctatcacac acctacgcta
     9541 acgtacgcat caaccatgtt ataaacctgt ggggagctga cttcgattct aacgggaacc
     9601 ttaaagctat ttatgtaaca gactctgata gtaatgcatc tattggtatg aagaaatact
     9661 ttgttggtgt taattccgct ggaaaagtag ctatttctgc taaagaaata aaagaagata
     9721 atattggtgc tcaagtacta gggttattta cactttcaac agggcaagat agttggaatc
     9781 agaccaatta aagcgtatca ataagaaaga atatcttcaa cagatgatgg tattctaagg
     9841 actaatacta ttgcagggaa tttttttata gatgggcaac agcaaaaaac attaataaat
     9901 cctataaaaa tcgataagga tattgttacg a
//
LOCUS       DAUGFP010000051         9526 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.051, whole genome shotgun sequence.
ACCESSION   DAUGFP010000051 DAUGFP010000000
VERSION     DAUGFP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.051"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            115..4221
                     /gene="cas9"
                     /locus_tag="VH663_001408"
     CDS             115..4221
                     /gene="cas9"
                     /locus_tag="VH663_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEQ8174599.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASRVDAKAILSARLSK
                     SRRLENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPEFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSDILKE
                     YPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWKQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVRKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNFGA
                     PAAFIYFDTTIGRNRYKSIKEVLDATLIHQSITGLYETRIDLSQLGGD"
     gene            4221..5090
                     /gene="cas1"
                     /locus_tag="VH663_001409"
     CDS             4221..5090
                     /gene="cas1"
                     /locus_tag="VH663_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ8174600.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLE
                     TTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLGKQMSWSETVK
                     SQVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTL
                     FGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFGLKHANQFNQFNFASDI
                     MEPFRPLVDKIVYENRNQPFPKIKRELFTLFSDTFSYNDKEMYLTNIISDYTKKVVKA
                     LNNEGKGVPEFRI"
     gene            5087..5428
                     /gene="cas2"
                     /locus_tag="VH663_001410"
     CDS             5087..5428
                     /gene="cas2"
                     /locus_tag="VH663_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ8174601.1"
                     /translation="MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSI
                     YSKLLLNNTANNAMIGRLREHNPNKGNITLLTVTEKQFARMIYLHGERNNCIANSDER
                     LVFLGEAFDES"
     gene            5418..6080
                     /gene="csn2"
                     /locus_tag="VH663_001411"
     CDS             5418..6080
                     /gene="csn2"
                     /locus_tag="VH663_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEQ8174602.1"
                     /translation="MNLNFSLLDEPIPLRGGTILVLEDVCVFSKIVQYCYKYEEDSEL
                     KFFDHKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVA
                     TITELIVFECLENELDLEYDEITILELIKSLGVKVETQSDTIFEKCLEILQIFKYLTK
                     KKLVVFVNSGAFLTKEEVASLQEYISLTNLTVLFLEPRELYDFPQYILDEDYFLITKN
                     MV"
     repeat_region   6183..6482
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6183..6218
                     /rpt_unit_seq="gttttagagctatgctgttttgaatggtcccaaaac"
     gene            6595..7056
                     /locus_tag="VH663_001412"
                     /pseudo
     CDS             6595..7056
                     /locus_tag="VH663_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            7126..8958
                     /gene="lepA"
                     /locus_tag="VH663_001413"
     CDS             7126..8958
                     /gene="lepA"
                     /locus_tag="VH663_001413"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEQ8174603.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDAKK"
     gene            9264..>9526
                     /locus_tag="VH663_001414"
     CDS             9264..>9526
                     /locus_tag="VH663_001414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEQ8174604.1"
                     /translation="MLTFGGASAVKADNQQLELAKKKNDLMTELHSNLEQMKSGTLPS
                     SNGDVEESTVKKILKYLEERDRLEDQWREKLFEGMQNKLFDGQ"
BASE COUNT         3182 a         1512 c         1983 g         2849 t
ORIGIN      
        1 tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa ctattagtga
       61 aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc aaaaatggat
      121 aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc ggtgatcact
      181 gatgattata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt
      241 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact
      301 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta
      361 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa
      421 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata
      481 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg
      541 gcagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt
      601 aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac
      661 aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac
      721 gcaagtagag tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta
      781 gaaaatctca ttgctcagct ccccggtgag aagagaaatg gcttgtttgg gaatctcatt
      841 gctttgtcat tgggattgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct
      901 aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt
      961 ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt
     1021 tcagatatcc taagagtaaa tagtgaaata actaaggctc ccctatcagc ttcaatgatt
     1081 aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa
     1141 cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat
     1201 attgatggag gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa
     1261 atggatggta ctgaggaatt attggcgaaa ctaaatcgtg aagatttgct gcgcaagcaa
     1321 cggacctttg acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt
     1381 ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa
     1441 atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt
     1501 gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc
     1561 gataaaggtg catcagctca atcatttatt gaacgcatga caaactttga taaaaatctt
     1621 ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac
     1681 gaattgacaa aggtcaaata tgttactgag ggaatgcgaa aaccagaatt tctttcaggt
     1741 gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag
     1801 caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga
     1861 gttgaagata gatttaatgc ttcattaggc acctaccatg atttgctaaa aattattaaa
     1921 gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca
     1981 ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc
     2041 tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg
     2101 tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt
     2161 ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg
     2221 acatttaaag aagatattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa
     2281 catattgcaa atttagctgg tagccctgcc attaaaaaag gtattttaca gactgtaaaa
     2341 gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa
     2401 atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgtga gcgtatgaaa
     2461 cgtattgaag aaggaataaa agaactagga agtgatattc taaaggagta tcctgttgaa
     2521 aacactcaat tacaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg
     2581 tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga tcacattgtt
     2641 ccacaaagtt tcattaaaga cgattcaata gacaataagg tactaacgcg ttctgataaa
     2701 aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat
     2761 tggaaacaac ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa
     2821 gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt
     2881 gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa
     2941 tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta
     3001 gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat
     3061 catgcacatg atgcgtatct taatgccgtc gttggaactg ctttgattaa gaaatatcca
     3121 aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt
     3181 gctaagtctg agcaggaaat aggcaaagca accgcaaaat atttctttta ctctaatatc
     3241 atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta
     3301 atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca
     3361 gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca
     3421 ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt
     3481 aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca
     3541 gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag
     3601 ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta
     3661 gaagctaaag gatataagga agttagaaaa gacttaatca ttaaactacc taaatatagt
     3721 ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attgcaaaaa
     3781 ggaaatgagc tagctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat
     3841 gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat
     3901 aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta
     3961 gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga caaaccaata
     4021 cgtgaacaag cagaaaatat tattcattta tttacgttga cgaattttgg agctcccgct
     4081 gcttttatat attttgatac aacaattgga cgaaaccgtt acaaatcaat taaggaagtc
     4141 ttagatgcaa ccctcatcca tcaatccatc accggtcttt atgaaacacg cattgatttg
     4201 agtcagctag gaggtgactg atggctggtt ggcgtactgt tgtggtaaat acccactcga
     4261 aattatccta taagaataat catctgattt ttaaggatgc ctataaaacg gagctgatcc
     4321 atttatcaga aattgatatt ttgttattag aaacgaccga tattgtcttg tccactatgc
     4381 tggtaaaacg gctagtggat gagaatgtcc ttgtcatatt ctgtgatgat aaacgattac
     4441 caacagctat gctgatgcct ttttatggtc gtcatgattc gagtctacag cttgggaaac
     4501 aaatgtcctg gtcagaaaca gtcaaatcgc aggtttggac gacgattatt gctcaaaaga
     4561 ttttgaatca atcttgctat ttaggagcat gctcctattt tgaaaaatcc caatctatta
     4621 tggatttata tcatggtttg gaaaattttg atccgagtaa tcgagaaggg catgcagcga
     4681 gaatttattt taatacactt tttgggaacg atttctcaag agatttggag catccaatca
     4741 atgcaggtct ggattatggt tatactttat tattgagtat gtttgcgcgt gaagtggttg
     4801 tgtctggatg tatgactcag tttgggctta aacacgctaa tcagtttaat cagttcaatt
     4861 ttgctagcga tattatggaa ccatttaggc ctttagtgga taagattgtt tatgaaaatc
     4921 gaaatcagcc ttttcccaaa ataaagagag agttatttac tttgttttca gatacatttt
     4981 catataatga taaagagatg tatctcacga atattattag cgattatact aaaaaagttg
     5041 tcaaagctct gaataatgaa gggaaaggag ttcctgaatt taggatatga gttatagata
     5101 tatgagaatg atacttatgt ttgatatgcc gacggacacc gctgaggaac gaaaagccta
     5161 tcgaaaattt cggaaatttt tacttagtga agggtttatc atgcatcaat tttctattta
     5221 tagtaagttg ctgttgaata atacagctaa caatgccatg attggtcggc tgagggagca
     5281 taatcctaat aaaggaaata ttacattact aacggtcacg gaaaaacagt ttgcacgaat
     5341 gatttattta catggtgaaa gaaataattg tattgcaaac tccgatgaaa gacttgtatt
     5401 tcttggggag gcttttgatg aatcttaatt tttccttact agatgaacca attccattaa
     5461 gaggcggtac aattcttgtg ctcgaagatg tctgtgtatt ttcaaaaata gtgcaatatt
     5521 gttacaaata tgaggaagat tctgaactta aattttttga tcacaagatg aaaaccatca
     5581 aagaatcaga aatcatgctt gtaacagata ttttaggatt tgatgttaac tcctcaacca
     5641 ttttaaaatt gattcatgca gatttagaat ctcaatttaa tgagaaaccc gaagtgaaat
     5701 cgatgattga caaattggtt gctacgatta cagaactgat tgtctttgaa tgcttagaaa
     5761 atgaattaga tttagagtat gatgaaatca caatcctgga attgattaag tccttaggag
     5821 taaaagtaga aacacaaagt gatactattt ttgaaaaatg tctagaaata cttcaaattt
     5881 tcaaatatct caccaagaaa aagttagtcg tctttgtcaa tagcggagct tttctaacaa
     5941 aagaggaagt ggctagttta caagagtata tatcattgac aaatttaaca gttctctttt
     6001 tagaaccacg tgaactatat gattttccgc agtatatttt agatgaagac tatttcttaa
     6061 taactaaaaa tatggtataa tattcttaat aaatgcagta atacaggggc ttttcgagat
     6121 tgaagtctag ctgagacaaa tagtgcgatt acgaaatttt ttagacaaaa atagtctacg
     6181 aggttttaga gctatgctgt tttgaatggt cccaaaacta tcactaactc cctattgcgt
     6241 ggggaactgt tttagagcta tgctgttttg aatggtccca aaactgtttc ttgcaatcgc
     6301 aatatttggg atgagtttta gagctatgct gttttgaatg gtcccaaaac ttagaggcct
     6361 atttcacaga caaagacatc gttttagagc tatgctgttt tgaatggtcc caaaacgact
     6421 catcccaact accgtaagcc ctaaaggttt tagagctatg ctgtcttgaa tggtctccat
     6481 tcaacattgc cgatgataac ttgagaaaga gggttaatac cagcactcgg ataccttcct
     6541 attctttctg ttaaagcgtt ttcatgttat aataggcaaa agaacagtag agtgatggaa
     6601 caaacatttt ttatgattaa gccagatggg gttaagcaag gggggttagt tggagaggtt
     6661 ttgcggtgga ttgaacgcct aagacttacg tttaagtgat tcgagctaag acaagctagt
     6721 tcgaaatact tggctaagca cgacgaggcc ttggtgataa accttttgat cctaaactta
     6781 aagcttacat gacaagtggt cctgttttaa ttaggacact tcttggggac taaggtggtt
     6841 ttgtcttttt gaaccatgat gagagtaacc aatcctacgg atgctctttg cggaaccatt
     6901 cgtgggaatt ttgctcaggc tcctcgtgat gatggaggtg tttttaacat ggcgtatggg
     6961 cctcattcaa gagactctgc taggcgtgag attttccttt ggtctcatca aagtaatctc
     7021 ggtagctccg ctattctctc acaagataat ccgtaagaaa agataggcaa aggcctacct
     7081 tttttgttat aatagaagga aagactaaat gtataggaaa atagaatgaa cagtcaagat
     7141 ttaaaaaaac gtcaggagaa gattcgtaat ttctccatta ttgcacatat tgaccatggc
     7201 aaatctactc tcgctgaccg cattttggaa aagacagaga cggtttcgtc tcgtgaaatg
     7261 caggctcagt tattggactc catggatttg gagcgtgagc gtgggattac catcaagtta
     7321 aacgccattg agcttaatta cacagctaaa gatggggaaa cctacatttt ccaccttatt
     7381 gataccccag ggcatgtgga ctttacttat gaagtatcgc gttcgttggc agcctgtgag
     7441 ggagcgattc tagttgtgga tgcagcgcag ggcattgaag cacagaccct tgccaatgtt
     7501 tacctagccc ttgataatga tttggaaatt ttaccggtta tcaataaaat tgatttgcct
     7561 gctgctgacc ctgagagagt ccgccatgaa gtagaagatg tcattggact tgatgcttct
     7621 gaggctgttc tggcgtcagc caaggctggt atcgggatcg aagagattct tgagcaaatt
     7681 gttgagaagg ttcctgctcc tacgggtgat gtggatgcgc ctttacaagc cttgattttt
     7741 gactctgtgt atgatgctta tcgaggcgtt atcctgcaag ttcggattgt gaatggtatt
     7801 gtcaaacctg gcgataaaat ccagatgatg tctaatggaa aaacctttga tgttacagaa
     7861 gttgggattt tcactcctaa ggcagttgga cgagatttcc ttgcaacggg agatgttggt
     7921 tatgttgcgg cttctatcaa aacggtagca gatacccgtg tgggggatac ggtgacttta
     7981 gctaataacc ctgctaagga agccttgcat ggctacaagc agatgaaccc gatggtcttt
     8041 gcagggattt atccgattga atccaataaa tacaatgact tgcgtgaagc gcttgaaaaa
     8101 ttacaactga acgatgccag tttacagttt gaacccgaaa cgtcacaagc gcttggcttt
     8161 ggttttcgat gcggcttttt aggcttgctt cacatggatg tgattcaaga acgtttagag
     8221 cgtgaattta acattgattt gatcatgaca gcaccatctg tagtctacca cgttcacaca
     8281 acagatgagg acatgattga agtgtctaac ccttcagaat ttccagaccc aacacgggtg
     8341 gcttttattg aagagcctta tgttaaagcg caaatcatgg tgccacaaga gtttgtaggg
     8401 gctgttatgg aattatcgca gcgcaagcgt ggtgattttg tgaccatgga ttacattgat
     8461 gataaccgtg tcaatgtgat ctaccaaatc ccgctggctg aaattgtctt tgatttcttt
     8521 gataaattga aatcctcaac gcgaggctat gcgagttttg attatgacat gtcagaatac
     8581 cgcaggtcac aattggtgaa aatggacatt cttttgaacg gtgataaggt cgatgcgctc
     8641 agctttattg ttcataaaga atttgcttat gaacgcggga aaattattgt tgagaaattg
     8701 aaaaaaatca ttccacgtca acagtttgaa gttccgatcc aagccgctat tggtcaaaaa
     8761 attgttgcgc gttcagacat caaggccctt cgtaaaaacg ttttggcgaa gtgttatggt
     8821 ggtgacgtgt ctcgtaaacg taaattgctc gaaaaacaaa aggctggtaa aaaacggatg
     8881 aaggctattg gttctgtcga agtcccccaa gaagccttct tgagtgtcct ttcaatggat
     8941 gatgatgcca agaaataacc aagcctaacc gcttagcctt cagaggagaa ctcttgtggg
     9001 tctacatgac catcttataa aaaaagaact ttacaatcat ttgcacataa tgttataatt
     9061 ctattatagt gttatctcat tattaggtga gcctacattt aatcttatta aagagaaaga
     9121 ggaaacaaaa actgtgaaca aaacaaaaca aaacaaaaca aaacaaaaca aaacaaaaca
     9181 aaacaaaaca aaacaaaaca aaacaaaaca aaacaaaaca tagccttctt tgtcgctatg
     9241 gcttaacctc agcggctgct cttttgctga cctttggagg tgcaagtgcg gttaaggcgg
     9301 ataatcagca actagagtta gcgaaaaaaa agaatgattt aatgactgaa ctccatagta
     9361 atcttgaaca gatgaaaagt ggaactttac ctagttctaa tggtgatgtg gaggaaagta
     9421 cggttaaaaa aatactaaaa tatcttgaag aaagagatcg attagaagac caatggagag
     9481 aaaaattatt tgagggaatg caaaataagc tttttgatgg tcaaga
//
LOCUS       DAUGFP010000052         8661 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.052, whole genome shotgun sequence.
ACCESSION   DAUGFP010000052 DAUGFP010000000
VERSION     DAUGFP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.052"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(170..2746)
                     /locus_tag="VH663_001415"
     CDS             complement(170..2746)
                     /locus_tag="VH663_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEQ8174605.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMSIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLTSMP
                     DAIYLFTLIKFGLIGLAACYSFHRLYPKISAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVVLGLNKLITENKTRTYYLSISLLFIQNYYFGYMIALFCILYALVCLLSLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPVKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIIL
                     TSLPYSFSQQYNFLPLTLFLLSVFLLLGYTISLFSFRNSQIPSTFISAFILIFSLLES
                     GLNTYYQLQGINKEWGFPSRQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDS
                     MKFNYYGISQFSSVRNRLSSSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSE
                     GPLNKFGFTKLKTSGNTTLYQNHYSSPLAILTRNVYKDVNLNVNTLDNQTKLLNQLSG
                     KSLTYFNLQPAQLISGANQFNGQISAQASDYQNSVTLNYQINIPKHSQLYVSIPNIIF
                     SNPDAKEVRIQTDNHNFIYTTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHF
                     YSLSIESYLEAMNSIKQKNVHTYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGK
                     NLPVKKAQGGFLSVTIPKGKGRVILTFIPNGFKLGLSLSCVGIIAYMLLYKYIDIKSK
                     LL"
     gene            complement(2829..4448)
                     /locus_tag="VH663_001416"
     CDS             complement(2829..4448)
                     /locus_tag="VH663_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ8174606.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATDDSILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(4527..5399)
                     /locus_tag="VH663_001417"
     CDS             complement(4527..5399)
                     /locus_tag="VH663_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ8174607.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMTSNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    5522..5763
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5818..6840
                     /gene="trpS"
                     /locus_tag="VH663_001418"
     CDS             5818..6840
                     /gene="trpS"
                     /locus_tag="VH663_001418"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEQ8174608.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
     gene            7148..8629
                     /gene="guaB"
                     /locus_tag="VH663_001419"
     CDS             7148..8629
                     /gene="guaB"
                     /locus_tag="VH663_001419"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEQ8174609.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
BASE COUNT         2911 a         1397 c         1641 g         2712 t
ORIGIN      
        1 agtagtttaa cattaaccct cacgaaaaat ctgaccttta tattatttga gtttattagt
       61 aaaatcgata aaagcatctc aagtattaga atatttgtct agctcttgag atgcttttag
      121 tatattgact catttgccaa attgataaaa aaatctgatt ttttttggat taaagtaatt
      181 tagactttat atctatgtac ttatacaaaa gcatataagc gataattcct acgcaagata
      241 gagataaccc taatttaaaa ccattaggaa taaaggtaag gataacacgt ccctttcctt
      301 taggaatagt aactgataga aatcctcctt gtgctttttt gactggaaga tttttcccat
      361 ctttttgtgc tgaccaacct ttatcgtaag gaagtgtaaa aataagagaa cctttcgttt
      421 ttgaattata atcagtgatt accgtattac ttttagcgta agtatgaaca tttttttgtt
      481 taatactatt cattgcttca aggtaagatt caatagacaa actataaaaa tgaggttcct
      541 taaaactaat ttgtttattt tttggaaaaa caaacgaaaa tgtagcaact ttggcatcgg
      601 cgaaatatcc taaatcaaaa aaagagtaag cgttatctgt agtatatatg aaattatgat
      661 tatctgtctg aatacgcacc tctttagcat caggatttga aaatataata ttgggtatgc
      721 taacatagag ttgactatgt ttagggatgt taatttgata attaagggta acggagtttt
      781 gataatcaga agcttgtgca gatatttgtc cgttaaattg attagcacca gaaataagtt
      841 gagctggttg taagttaaaa taggttaaag atttcccact tagttggtta agtaatttgg
      901 tttggttatc aagggtattg acatttaggt tgacatcttt gtaaacatta cgtgttaata
      961 tagctaaagg gctactatag tgattttgat aaagagtagt attcccgcta gtttttagtt
     1021 ttgtaaatcc aaatttattt agaggtcctt cgctaagatt atattttata ccaagtagac
     1081 tgtccataat aatagtattg ttttggtatc taaggtttaa atttgtgcct ttagactgaa
     1141 atcccaatcg atccaataaa gaactagata gtctatttct tacagaggaa aattgtgaaa
     1201 tgccgtaata attaaatttc atgctatcgt tccctgtttg gggaagtagc ctttccattc
     1261 taaaaaaagg ttgactattt tttgacacag agttgacaag gttgttaata tcctttaatt
     1321 gactattata tatctgtcgt gatgggaatc cccactcctt attaattcct tgaagctggt
     1381 agtaggtgtt taaccctgat tcaagaaggc taaagataag tatgaaagca gaaataaaag
     1441 tagatgggat ttgagaattt ctaaacgaaa atagtgaaat agtataacct aataataaaa
     1501 aaacactaag taaaaaaaga gttaaaggta gaaaattata ttgttgagaa aagctataag
     1561 gaagagatgt cagtataatg aggaaaataa aagcaaaacc tgcttttatt tgagtcactt
     1621 cttttagtcg agagagagtc tcacatgcga gtaataggat aactattgaa aaagaccaag
     1681 cgtagcgatg caaaaacata tttggtgagt gcatcccctg ccaaaaaaga tcaagtggct
     1741 gtaggtaaaa acttattata ataaaagtta acaagcaggc attggctaat tttattttta
     1801 aagggatact ttctaaagta aaataaataa cactaagcat taggggaaat aatcctacgt
     1861 aaatcatagg cagagcatta aacttggtag tatcgtacac tcctattgag agcttagcag
     1921 gtatatccaa aaaccaagca ttgttcgtaa ctaactgttt taccggggat agattctctc
     1981 cataagttga caaatctaga taggtaggaa gtattactag agcacttgtt aaagcagcac
     2041 atattgacac agctgtaaac ctaacaaaag cgataaacat tttgttaaaa tcattcagac
     2101 ttaaaagaca aactaaggcg taaagaatac aaaaaagagc aatcatgtag ccaaagtagt
     2161 aattttgaat gaataataat gatatcgaaa gataataagt tctagtttta ttttctgtga
     2221 taagtttatt taatccaagt acaacaagtg gaagaagaat gaaaacatct aaccaagaat
     2281 ttagttccat ttgacttgtc aagaagctca ttaagctata aaaaactgag atggaaatca
     2341 tcaagaaagc actgattttt ggatataatc tatgaaaaga atagcatgca gctaatccta
     2401 ttaacccaaa ttttatcaag gtaaacaaat agatagcatc tggcatagag gttaaattaa
     2461 aaaagaaaag taatggagaa aagaaactgc caagataata acacattaaa gcataaaaat
     2521 ttatccctag tccgcttgta aaggtataaa aaaaactatc agaaccgtgc atgatgttac
     2581 gaaagttctg cgcaaaaata acatactgat gaaaagcatc actagctaga attgttttat
     2641 cactattata ataaatgctc atcgatagaa ggatgataaa tataatacta agagggaaca
     2701 aaaaactagc aagtccagca attatccatt tattattatt tttcataaat tatatggtct
     2761 ttcgtttaca aattttaaaa aagaggctag gaagagatct tcttagccac ctcatttatc
     2821 ataaggtttt atttccaaag ttcagcaact cgggcttgga cttctggatt gtcaaggaat
     2881 tcgtcataag tttcatctat acggtcgata acaccatttt ttgaaataac aacgatgtgg
     2941 ttagcaatcg tttgaataaa ttcatggtca tgactggcaa agataacaga ttctttgaag
     3001 tctttgatac catcattaag gcttgaaata gattccaaat ctaagtgatt agttgggtca
     3061 tcaagaataa gaacgtttga tttcaatagc atcaatttag aaagcatgac acgcactttt
     3121 tcgccaccgg aaagaacgtt aacagatttt ttaacttcgt ctccagagaa aagcatacgt
     3181 cctaggaaac cacgtaaaaa ggtatcatca tcttcacctt ttgaggcgaa ttgtcgtaac
     3241 cattcaagga tggaatcatc tgttgcaaaa tcttttgaat tgtcttttgg caagtaagaa
     3301 cgacttgttg tcactcccca tttaatggtt ccttcgtagt caatatcatc tgctaaagca
     3361 cgcataagag cggttgtttg aatatcattt tgaccaataa tagcagcttt gtcacctgga
     3421 cgtaaaatga aactgatatt atcaatgatt ttctctccat cgatcgttac agaaagattt
     3481 tcaacagtga ggaagtcatt tcccatttca cgctctgctt taaaattaat aaatgggtat
     3541 ttacggcttg agggaacaat ttcttctaat tcaatcttat caagcatttt tttacgagag
     3601 gttgcttgtt ttgatttaga tgcgtttgct gagaaacggg caacgaattc ttgtaattct
     3661 ttgatttttt cttcggcttt agcattgcgg tcagcttgta aacgagcggc tagctcagaa
     3721 gattgtttcc agaaatcgta gttacccaca aagagtttga ttttaccaaa gtcaaggtcg
     3781 gccatgtgag tacatacttt attcaagaag tgacggtcat gggaaacaac gatgactgta
     3841 ttttcaaaat caatcaagaa atcttccaac catgaaatag attgaatatc aagaccattg
     3901 gttggctcgt caagtaataa aacgtcaggc ttaccaaaga gcgctttagc aagaaggact
     3961 ttcactttat ctccattagc cagttcactc atattttggt agtgaaggtc ttctggaata
     4021 tttagatttt ggagaagttg agaagcttca ctttctgcct cccagccacc taattcagca
     4081 aagatacctt ctagctcagc ggcacgaacc ccatcttctt ctgaaaaatc tgctttcata
     4141 tagatagcgt ctttttcttt catgatattg taaagttgct cattacccat gattacaaca
     4201 tcaatcgcac gttcttcttc ataatcaaaa tgattttggc gaagaacaga taagcgttcg
     4261 tcaggtccta aagagatatg accagtactt ggttcaatat ctcctgcaag gattttaaga
     4321 aaagttgatt ttccggcacc attcgcaccg attaagccgt aagtattgcc ggctgtgaat
     4381 ttgatattaa catcatcaaa tagttttcga tcactaaagc gtagtgatac atcagaaact
     4441 gtaagcaatt tgtcacctca ttttatttat ctaagcttca ttgtacctaa aatagtcccc
     4501 tttttcaatc agaaaagggc taatcactac ataggcaata aaacgtcttt gtcttcacgt
     4561 ttgtattgtt tagtcaaact aaatcctctt cccataactt gagctgctgg catgacaaca
     4621 ataaaggctg tgtcgtcaat ttcgaggatt ttttcttgga gagtaggtaa ttcgtgactt
     4681 gatacaatag ccattaacat aattttatca gaagtagtat aaccaccgcg aataggaagt
     4741 ttagtcactc cacgatccat aacagttgtg atatattctc gaatagcttg ataatctttg
     4801 gaaataatca taacattttt ggatgagtca aagccatttt ccataacgct aatcacataa
     4861 ccaattacga aaagtccaat cgtagaatac atcacgtcat ctgcagaaag tgcaataaat
     4921 cccataagga cactaatacc atctacaatt gtcattgcaa ctccaagaga taaaggagag
     4981 tatttatgaa gtatttgtgt tagaattcca gttcctcctg tcgaagagtt tccccaaaaa
     5041 accattccta aaccaatgcc acagataata ccaccaaaaa tagctgctaa caactgattg
     5101 tgtgtcagag taggtaatga attggttgaa cggatggcaa taggatatat ccaagaacca
     5161 taaagtgttt taataaaatt ttgttttcca agaaagaagt aacacattaa taataagggg
     5221 atattactag tcattaaaaa aagcgacgga ctaatgccaa atagagcttt tataacaact
     5281 gcaataccaa ccataccccc tgatgcaata tgattgtcga caaacattgt attgaaacca
     5341 atagctgtta tgaatgagcc aattgtgaca agtagtaaat caatccctcg ttttttcatg
     5401 cttttaatct cctttataac tggttaaaaa tagtagactc attatctctt aaatgtactg
     5461 agaccgtcaa gtcatatttt caaaaaatct ggtgaacttg acaagaagtt taaaatttca
     5521 tagaataaat ctaaattaga agagtattct tttcatgttt ttagagagtt agtggttggt
     5581 gcaagctaac aacagaaaag gtgaaatgga ctaatgatta tttgaaagga aaccataact
     5641 ttcaggttgg caccccttac cgtgcagctt ctttcattga gaagattgag atagtatctc
     5701 gttttgataa agcagatgct aattgaggtg gtaccgcgta ttacttgtaa taacgccctc
     5761 acgttttaat agcgtgggga ctttttgcta tatcaagaga tagttaggga gaaaatgatg
     5821 acaaaaccta ttattttaac tggagataga ccaactggaa aattacattt aggtcattat
     5881 gtcggaagtc ttaaaaatcg tgtcttttta caaaatgaaa acaagtataa gatgtttgtt
     5941 ttcttggccg atcaacaggc actgacggat catgctaaag aatccgaatt aattcaagaa
     6001 tctattggaa atgttgcctt ggattacctc tctgtcggtt tggatccgaa gcaatcaacg
     6061 atttttattc aaagtcagat tccagagcta gctgaattga gcatgtatta tatgaatctg
     6121 gtatcattag cacgtttgga aagaaatccc actgttaaaa ccgaaattgc tcagaaaggt
     6181 tttggcgaaa gtatcccatc tggttttttg gtttatccag tatcacaggc cgctgatatt
     6241 acagcattta aagcgaattt agtacctgta ggtaacgacc agaaaccgat gattgaacaa
     6301 acacgtgaaa ttgtgagaag ttttaatcat acttaccata cagactgttt agtagaacct
     6361 gaaggtattt atccagaaaa tgaaaaagct ggacgcttac ctggtcttga tggcaatgcc
     6421 aaaatgtcta aatcattggg aaatggaatc tatctctcag atgatgcaga taccgttcgc
     6481 aaaaaagtga tgagcatgta tactgatcca aatcatatta aaatagaaga tcctggtcaa
     6541 attgaaggga atatggtctt tcattatttg gatatttttg ctagaaaaga agatcaagct
     6601 gatatcgaag caatgaaaga gcattatcaa agaggtggtt taggagatgt gaaaacgaaa
     6661 cgctaccttc tagatatttt agaacgtgaa ttagcaccta ttcgtgaaag acgtttggag
     6721 tacgctaaag atatgggaga ggtgttccgt atgttacaag aaggtagtca aaaagcaaga
     6781 actgtggcag ccaagacttt atcagaagtg aagtcagcaa tgggtattaa ttatttttaa
     6841 tgttttagag aaaaagctaa gttagtaatg ataagataat tactattatc aaagcaggac
     6901 ctaatttgtc atttctttac aggatagtag agattatgtt ttatatagtc ttatttttga
     6961 ttaaaaataa gacccaaatg aattttttga ttaataaaaa acggataata ctgatatatt
     7021 atgctgatag tgacttcagc tgtaggaata ttttattgac aaatgatttt agcgtgatat
     7081 gatagtaaca ataaaaaata gaccagggta atattcccta aaaataaaag aaaagaggat
     7141 tatctggatg tcaaattggg acactaaatt tttgaaaaaa ggctacactt ttgacgatgt
     7201 cttgcttatt ccggcagaga gtcatgttct tccaaatgaa gttgatttaa agacaaaact
     7261 cgcagacaat ttgacattaa atattcctat tattacagca gctatggata cggttactgg
     7321 tagtaaaatg gctattgcaa ttgcgcgtgc gggtggttta ggtgttattc ataaaaacat
     7381 gtctattact gagcaagcag aagaagttcg caaggtaaaa cgttctgaaa atggtgtcat
     7441 cattgatcca ttcttcttaa cacctgaaca caaggtttca gaagctgaag aattaatgca
     7501 gcgctaccgt atcagtggtg tgccaattgt tgaaacttta gcaaaccgta agttggtcgg
     7561 tatcattact aatcgtgata tgcgttttat ttctgattac aatgcaccaa tctctgaaca
     7621 tatgactagt gagcacctcg ttacggctgc tgttggtaca gatcttgaaa ctgcagaacg
     7681 tattcttcat gaacaccgta ttgaaaaact tcctttggtt gataatagcg gtcgtctgtc
     7741 tggtttaatc actatcaaag atattgaaaa agtaatcgaa ttcccacatg ctgctaaaga
     7801 tgaatttggc cgtcttctgg tcgctgctgc ggtaggtgtt acctcagata cttttgagcg
     7861 tgccgaggcc ttgtttgaag ctggtgcaga tgcgattgtt attgacacag ctcatggtca
     7921 ttcggctggt gttcttagaa aaattgctga aattcgtgct catttcccaa atcgtacttt
     7981 gattgctgga aatattgcca ctgctgaagg tgcgcgtgct ctttatgatg caggtgttga
     8041 tgttgttaaa gtgggtattg gtcccggatc tatctgtacc actcgtgtgg ttgcaggtgt
     8101 cggtgttcct caagtgactg ctatttacga tgctgctgct gttgctcgtg aatatggcaa
     8161 aacgattatt gctgatggtg gtatcaagta ttctggcgat attgttaagg ctcttgcagc
     8221 cggcggtaat gctgttatgc ttggatcaat gtttgcaggt actgatgaag ctccaggtga
     8281 aactgaaatc taccaaggcc gtaagttcaa aacttatcgt ggaatgggtt ccattgcagc
     8341 tatgaagaaa ggatcaagcg atcgttactt ccaaggttcc gtaaatgaag ctaataaact
     8401 agttccagaa ggtattgaag gccgtgttgc ttataaagga gctgcttcag atattgtctt
     8461 ccaaatgtta ggtggtatcc gctcaggtat gggctatgtt ggcgcaggtg acattcaaga
     8521 attacatgaa aatgctcaat ttgttgaaat gtcaggtgca ggcttgattg aaagtcatcc
     8581 gcatgatgtc caaatcacaa atgaagcacc aaactattct gtgcactgat aatttaattt
     8641 agtaacttta agggctgaca t
//
LOCUS       DAUGFP010000053         8626 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.053, whole genome shotgun sequence.
ACCESSION   DAUGFP010000053 DAUGFP010000000
VERSION     DAUGFP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.053"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            24..3239
                     /gene="rexB"
                     /locus_tag="VH663_001420"
     CDS             24..3239
                     /gene="rexB"
                     /locus_tag="VH663_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEQ8174610.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVVVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFLKKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELISFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIKVIDQE
                     KNFHLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLVTYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMGQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            3200..6868
                     /gene="addA"
                     /locus_tag="VH663_001421"
     CDS             3200..6868
                     /gene="addA"
                     /locus_tag="VH663_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEQ8174611.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERYSDQSQKRTAQ
                     QIEAIYTSGQNILVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLCQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFSEFMNQKEAPAFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKYTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTDLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDIAHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGNVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYGIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNVFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQD
                     ITDPKKLGKLLPLALREQLLTFQDWLLAIADIFSTEDLYFDVRFIEDSDLTQESVGRL
                     QIPQLLNADDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKAAYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKAKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALRGIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
     gene            7007..7819
                     /locus_tag="VH663_001422"
     CDS             7007..7819
                     /locus_tag="VH663_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ8174612.1"
                     /translation="MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAV
                     SPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKAD
                     LAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAVQKGSIE
                     EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLETPVAAGFLAQHKDLALAPFSL
                     KTSDGDAKAVALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
     gene            7960..8136
                     /locus_tag="VH663_001423"
     CDS             7960..8136
                     /locus_tag="VH663_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEQ8174613.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(8264..8626)
                     /gene="mscL"
                     /locus_tag="VH663_001424"
     CDS             complement(8264..8626)
                     /gene="mscL"
                     /locus_tag="VH663_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEQ8174614.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAV
                     APTQEELLTEIRDLLAQK"
BASE COUNT         2927 a         1458 c         1589 g         2652 t
ORIGIN      
        1 ttcagtttgt ataaaggagt catatgaaat taatttacac agagatgagt tactcgatga
       61 cagagatttt ggtgaatgaa gcacgaaaag ctgctgacca aggttatcga gtgttctata
      121 ttgctccaaa ttctctttca tttgaaaagg aaagagaagt cttaacctta ttgcctgaac
      181 gagggacttt ttcgataata gttacccggt tcgttcaaat gtcaaggtat tttacagttg
      241 agagctctcc ttctaagcaa catctagatg acacgacttt ggctatgatt ttttatcggg
      301 ccttgatgca attaaaacca gaagatttgc cgagttatgg tcgtttgcaa aataattcgg
      361 tttttattga acaactagtt gagttatata aggaattaaa aaacgcacaa ttaagcgtac
      421 atgatttaac aggattagac catcctcaaa aacaagaaga tcttattaaa attattgagc
      481 ttgcagagac gatcatgatt cagcaagatt ataatcaaga tagtcctcta caatcttttg
      541 caagagcaat taagttagga cttttaaata accaactatc aaaaacagtt gttgttattg
      601 atggtttttc acgtttttca gctgaagagg attatttatt atcactttta aataacaact
      661 gtcaagaggt catcattggc agttatgtga gtcaaaaagc ttatcaaaaa tcttttatta
      721 agggaaatat ctatgaggct agccttcatt ttttgcagga tttggctcaa aaataccata
      781 tcaagccggt atttgcgacc tctaatcaag tgtttaaacc agccttttca cggctaacac
      841 aattatttga agcaactcat gatttttcac aagtagactg gcaactccaa aagaatgatc
      901 ttgatcactt tagcctttgg caatgtcatc atcaaaaaga ggaaattgaa catgtagcaa
      961 aatccattcg gcaaaagcta tatgaaggtt accgttataa ggatatctta gtgttgctag
     1021 gagatatgga tgcttatcaa ttacaaattg ggcctatctt tgataagttt gaaatcccct
     1081 actatttagg aaaagcagag cctatggctg cccatccatt agtccaattt atagaatcgc
     1141 tcgaacgaag tcagcgctac aattggcgtc gtgaagacat tttaaacatg ttaaaatcag
     1201 gactttttgg ttgttttgat gatagtgata ttgatcgctt tgaggaatat actcagtttg
     1261 cagatattaa agggtttact aaattttcaa agccttttac cattaactct tctaggcagt
     1321 atcctcttga ctttttaaat gaaatgagac aagacatcgt tttacctttg caagaattgt
     1381 ttaagagtca aaaacaactt ggtgcttcac ttgttgacaa attaatactt tttttaaaaa
     1441 agattagatt agctgagaat atgcaaggtt tagcacagtc ccaattagag gtggaaaaaa
     1501 acgaagaagt ttggaaaagg tttacagata ttttgacttc ttttcatcat atctttggcc
     1561 aagaaaaatt acggctatca gattgccttg ctttaatcaa aacgggaatg aaatcagctc
     1621 agtatcgtgt tgttccagcg acattagatg ttgtcactat taaatcttat gaccttgttc
     1681 aaccacattc aaaacctttc gtttatgcaa ttggtctaac tcaatcacat tttcctaagc
     1741 aaattcacca ttcaggacta ctttctgatc aagagcgtgc tagaattaat gaaataagaa
     1801 attatcgtca ttttgatatt gctagtgccg aaaatagtaa aaaaaaccat caaactgcgt
     1861 tatctctgtt taatgctgct actaaagagt tagttcttag tgttccaacg gtaatcaacg
     1921 agacattcga tgatctatct ccttatctta aagaattaat aagctttggg ctacctcttt
     1981 tggataaagg caaaaactat ctatcttatg ataatagcga tattggtaac tataaagctc
     2041 tcttgtcaca aattattgct attaaccgac aagatcttat agaaatgtca gatcaagata
     2101 agatgttttg gactgttgtc ttacgttatc tcaggaaaca gttaagaaag caacagctag
     2161 agttacctac ttcagattat cgtttatcaa caaagccttt gtctaaagaa gttatcgagg
     2221 tatgttttcc aaagggaatc cctttaaagc tatctgcaac tgctttaacc gttttttata
     2281 ataatcagta caactatttt ttgaaatatg ttttgaattt aaacaaaaca gaatccattc
     2341 atccagattc cagaattcat ggtcaatact tgcatcgtgt ctttgagcgg ttaatgaaag
     2401 atcacacgca agaaccgttt gataataagt taaaacaagc aatctaccat accaatcaag
     2461 aaagtttttt tcagcaggtt taccaagata atgccgaggc tgagtacagt ttagctatac
     2521 tagaagatat tgttagatca acagcaccta tcttgcaact taaccaaaac ataaaggtca
     2581 ttgatcaaga aaagaacttt catctggata tgggaaatga gatattagtc cacgggatca
     2641 ttgatcgtat tgaccagctg agcgacggta gcttgggcat tgttgactat aaatcaagcg
     2701 cgaatcaatt tgatattggg accttttata atgggttgag tccacaacta gtgacttacc
     2761 tcgcagcctt aaaacagatc gcaccgcacg atataaacca attatttggg gctatgtacc
     2821 ttcatttgca agatcctaaa ttggatctag taacatttaa acaaattgat aacacattag
     2881 tggagagtat ctataaagcg ttgacctata aaggtatttt ttcagaagtc gaaaaagagc
     2941 atctatctac tggtgcttac caaaccaaaa atgctcttta ttccaatgac gaattggaaa
     3001 cacttttaaa ttataacaag tacctctatc ttaaagctgc taaacacatt aaaaagggac
     3061 attttttgat taatccttat acaagtgatg gtaagacagt tcaaggagat caattaaaag
     3121 ccattacccg ttttgaagca gatcttgaca tgggacaggc aaggcgacta gtgactctcc
     3181 ctgctaagga gaaaaaagag tgctttttaa cattaatgag aaaggagagc cacttgtgat
     3241 ttcttttgcc ccatttttaa gccccgaagc tattaaacat ttacaagaaa acgaaaggta
     3301 cagtgatcag tctcaaaaac gcacagctca acaaattgaa gcaatttata ctagtggcca
     3361 aaatatactt gtatcagctt ctgctggttc aggaaaaacc tttgtaatgg tcgaacgcat
     3421 acttgataaa attttgagag gtgtttcaat tgatcggctt tttatctcaa cctttactgt
     3481 taaagcagct acagaactgc gtgaacggat tgaaaacaaa ttatactcac aaattgctca
     3541 aactacagat tttcaaatga aagtttattt aacagaacaa ttgcaatctc tttgtcaagc
     3601 tgatattggt actatggatg cttttgcaca gaaagtagta agtcgctatg gttatagcat
     3661 tggcatttca tcccaatttc gtatcatgca ggataaagca gaacaagatg ttttaaagca
     3721 agaggtgttt agcaaactct ttagtgagtt tatgaatcaa aaagaggcac cggcgtttag
     3781 ggctcttgtg aaaaattttt ctggtaactg taaagacact tcagctttta gagagttagt
     3841 ttatacttgt tattctttta gccaatcgac agaaaatcca aaaatatggt tgcaagaaaa
     3901 ttttctaagc gctgctaaaa cttaccaaag acttgaagat atcccggatc atgatattga
     3961 actcttactt ttggcaatgc aagacactgc aaatcagcta agagatgtga ctgatatgga
     4021 agattatggg cagctgacta aggcaggtag ccgatctgct aaatacacta aacacttaac
     4081 gatcatagaa aagttgtctg attgggtgcg tgattttaaa tgtttgtatg gaaaagccgg
     4141 attggatcgg ttgatcagag atgtgacaga ccttatacca tctgggaatg atgttacagt
     4201 ctcgaaggta aaataccctg tttttaagac cttgcatcaa aaattaaaac aatttaggca
     4261 tttagaaaca attttaatgt atcagaaaga ctgttttccc ttattggaac agttacaaga
     4321 ttttgtgctt gcgttttcag aagcttattt agctgtcaaa atacaagaaa gcgcttttga
     4381 attttcagat attgcacact ttgcaatcaa aattttagag gaaaatacgg atattcgcca
     4441 atcctatcag caacactatc atgaggtgat ggttgatgaa tatcaagata acaatcatat
     4501 gcaagagcga ctcctgacct tactatcgaa cggtcataat cgctttatgg taggagatat
     4561 caaacaatcg atctatcgat ttcggcaagc cgatcctcag atttttaatc aaaagtttag
     4621 agactatcaa aaaaaacctg agcaggggaa agtgatttta ctcaaagaaa actttcgtag
     4681 ccaatcagag gtgttaaatg tcagcaacgc tgtttttagt cacttaatgg acgaatcagt
     4741 aggaaacgtc ttatatgatg agcaacatca gttaatagca ggtagtcatg ctcaaacagt
     4801 cccctatcta gaccgtcgtg ctcagttatt gctatataat agcgataaag atgatggcaa
     4861 cgccccttca gatagtgagg gtatttcatt tagtgaggtt acaattgttg ccaaagaaat
     4921 tattaagctt cacaatgata agggtgtccc ttttgaagac attacgttac tcgtttcttc
     4981 aagaacaaga aatgatatca tttctcatac attcaatcaa tatggtattc ctatagtaac
     5041 agatggtggg cagcaaaact atcttaaatc tgttgaagtg atggttatgt tagatacatt
     5101 acgcaccatt aataacccaa gaaatgatta tgcccttgtg gctttactgc gctcaccgat
     5161 gtttgccttt gatgaggatg atttagcaag aatagcactt caaaaagaca atgagctaga
     5221 taaagattgc ctatatgaca agatacaaag ggctgtgatt ggaagaggtg ctcatcctga
     5281 attgattcac gataccttgc ttggcaagtt aaatgttttt ttaaagacgt tgaaaagctg
     5341 gcgtcgatac gctaagctag ggtcgttgta tgacttgatt tggaaaattt tcaatgatcg
     5401 tttttatttt gattttgtag ctagtcaagc aaaagcagaa caagcacaag ctaatctata
     5461 cgcattagct ctacgtgcta atcagtttga aaaatcgggc tataaagggc tgtaccgttt
     5521 tattaaaatg attgataagg tacttgagac gcaaaatgac ttagcagatg tggaagtggc
     5581 tgctcctaaa caagctgtta atttaatgac cattcacaag tctaaaggtt tacaatttcc
     5641 gtatgtattt atccttaatt gtgacaagcg cttctcaatg acagatattc ataaatcatt
     5701 tattctgaat cggcagcacg gtatcggtat caagtacctt gcagatatca aaggtttact
     5761 tggtgaaaca acactcaatt ctgttaaagt aagcatggaa accttacctt atcaattgaa
     5821 caaacaagag ttacgcttag caactttatc agaacaaatg cgcttactgt atgttgctat
     5881 gacacgagct gaaaaaaaag tttattttat tggtaaagct agtaagagca aaagtcaaga
     5941 tatcacagat cctaaaaagt taggcaaact tttgccgctg gctttacgag aacagttatt
     6001 gacattccaa gattggctat tagcaatagc agatatattt tcaactgaag atctttattt
     6061 tgatgttcgc tttattgaag atagtgattt gacacaagag tcagtcggac gacttcagat
     6121 accacagtta ttaaatgcag atgatcttaa agataatcgt caatcagaaa caattgcacg
     6181 ggctttagat atgttagaag cagtgtctca attgaatgcc aattatgaag cagctattca
     6241 tttgccaaca gttcgaacgc ctagccaact taaggcagct tacgagcctt tattagaacc
     6301 cattggtgta gatattatag agaaatcttc tcgatcgcta tctgatttta ctttgccaca
     6361 tttttcaaaa aaagcaaaag ttgaagcaag tcatattgga tcagctcttc atcagttgat
     6421 gcaggtgctc cctttgtcaa aaccgataaa tcaacaaacg cttttagacg ctttaagagg
     6481 aattgatagt aacgaagagg taaaaacagc tcttgatctc aaaaaaatag agtcgttctt
     6541 ttgtgataca agcctaggcc aattttttca gacttaccaa aaacacttgt atcgagaagc
     6601 gccatttgct attttaaaag ttgaccctat tagtcaagaa gaatatgtcc tacgtggtat
     6661 tatagatgcc tactttttgt ttgatgatca tattgtatta gtggactata aaaccgataa
     6721 atacaagcag cccattgagt taaaaaagcg ttaccaacaa cagttggagt tatatgcaga
     6781 agctctcact caaacgtata aacttcctgt gactaagcgc tatcttgttt taatgggagg
     6841 tggaaagcca gaaattgtcg aagtttaagg ttgttgtgac acaagggtat tttgcaaatg
     6901 ataatagtag agttgaggat tttacgaaat gcttgaaaag ctattgacct ttgaataata
     6961 atacaatata ataagtttta ttagcgaaaa aaggaataaa tatactatga attttaaaaa
     7021 aataagattt ggttttttgc tttgcctcct actttatcct ttagcagctt gtaacaagtc
     7081 tgaacaactt aatcactacg aaagaattaa aaaaacaaga aaattagtcg ttgctgtaag
     7141 ccctgattat gctccttttg aatttaaagc tttagttaat ggaaaagata ctattgttgg
     7201 agcagatgtt caattagctc aggctattgc tgatgaatta gacgttgatc ttgagctttc
     7261 accaatgagt tttgataatg ttttgtctag tttacagact ggtaaagctg atcttgctat
     7321 ttcaggaatc tcacatacta aagaaagagc taaagtatat gatttttcaa ttccttacta
     7381 tcaggcagaa aacgctatag tgatgagagc atctgatgct aaagtgacca aaaatattag
     7441 tgacctaaac ggtaagaaag tagccgttca aaaaggtagt atcgaggaag gtttagttaa
     7501 aatacaatta ccaaaggcca atctgatttc tttgactgct atgggagaag ccattaatga
     7561 actaaaagca ggacaagtct atgcagttac cttagaaaca cctgtagctg ctggtttttt
     7621 agcccaacat aaggatctgg ctttagcacc ctttagctta aaaaccagtg atggagatgc
     7681 caaagcagtg gcccttccta aaaatagtgg tgacttaacc aaagccgtta ataaggttat
     7741 tgctaagtta gatgagcaag aacggtacaa gtcatttatt gcagagacaa tagcactaac
     7801 gaaaaatact atgaagtaat aaggttaata aaaactttat tagtatccgt tggaaaagtg
     7861 tttggaataa acaataaaag aggtttgact ttttttaaaa aataatgtaa tataattggg
     7921 aacgtgaaaa tgttacgata tcttgaggag gtgaaacaca tgtcaaaaac agtagtacgt
     7981 aagaatgaat ctcttgacga tgctcttcgt cgcttcaaac gttctgttac taaagctggt
     8041 actcttcaag aatcacgtaa acgtgaattc tacgaaaaac cttctgtaaa acgtaaacgt
     8101 aaatcagaag cagctcgcaa acgtaaaaaa ttctaatggt aaaaaataaa aacagttctc
     8161 acaattagaa ctgtttttat tttttatcca gaagaagcaa gggagctttt ttgatatcaa
     8221 aaaaagcagt tagacatgcc taactgcttt atcaaaaatc gccttatttt tgagccaaca
     8281 aatcacggat ttctgtcaat aattcttctt gagtaggagc tacagcttct ttttcttttt
     8341 taggcatagc tttttcagcg gcttttacaa caaagaaaag actggtaccg atgataagaa
     8401 aattaataac ggctcctaaa aagctaccat atttaacacc attccatgac aattgagtga
     8461 tattttcaac atttgcagct ttcagagctg ggtttaaaat aagcggtgtg ataatatcgt
     8521 tcacaaaaga agtgacaata gcaccaaaag ctcccccgat gataactgct acagcaagtt
     8581 caataatgtt tcctctaaac aaaaatgctt ttaattcttt aaccat
//
LOCUS       DAUGFP010000054         8535 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.054, whole genome shotgun sequence.
ACCESSION   DAUGFP010000054 DAUGFP010000000
VERSION     DAUGFP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.054"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(39..743)
                     /gene="speG"
                     /locus_tag="VH663_001425"
     CDS             complement(39..743)
                     /gene="speG"
                     /locus_tag="VH663_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeG"
                     /protein_id="HEQ8174615.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLKDLKRSLRFAYNITPC
                     DYENVEIAFVTTNSIHINTKQKRSECILYVDSIVSLGITDQFIKGDKVDVFGLPYNFS
                     PPYVDNIYGGIVKHSNQGNKSLQFVGILNQDGKETYLPSEAVRIKKKQFTLQEFDFKI
                     RKFLMEKYNIYDSESRYTSGSLFLATKDSKHYEVDLFSKDDKLLSRDSFFKRYKDNKI
                     FNSEEISHFDIYLKTY"
     gene            complement(845..943)
                     /gene="ffs"
                     /locus_tag="VH663_001426"
     ncRNA           complement(845..943)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VH663_001426"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1143..2375
                     /locus_tag="VH663_001427"
     CDS             1143..2375
                     /locus_tag="VH663_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEQ8174616.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANMPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQAQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDHLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEPLSLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(2989..3504)
                     /locus_tag="VH663_001428"
     CDS             complement(2989..3504)
                     /locus_tag="VH663_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEQ8174617.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(3504..4283)
                     /locus_tag="VH663_001429"
     CDS             complement(3504..4283)
                     /locus_tag="VH663_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ8174618.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKIVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PEGINSKNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(4423..4962)
                     /locus_tag="VH663_001430"
     CDS             complement(4423..4962)
                     /locus_tag="VH663_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEQ8174619.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTKQ"
     gene            complement(4966..5277)
                     /locus_tag="VH663_001431"
     CDS             complement(4966..5277)
                     /locus_tag="VH663_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174620.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQETSPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(5495..6637)
                     /gene="tgt"
                     /locus_tag="VH663_001432"
     CDS             complement(5495..6637)
                     /gene="tgt"
                     /locus_tag="VH663_001432"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEQ8174621.1"
                     /translation="MTDYPIKYRLIKTEKHTGARSGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVVKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            6856..7719
                     /locus_tag="VH663_001433"
     CDS             6856..7719
                     /locus_tag="VH663_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEQ8174622.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGLILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     GNLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGSSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDKVKEGTYLGAIAVIETSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
     gene            7916..8535
                     /locus_tag="VH663_001434"
                     /pseudo
     CDS             7916..8535
                     /locus_tag="VH663_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2720 a         1713 c         1524 g         2578 t
ORIGIN      
        1 tcatttgaat acttaaaaag caaaggggga gaatagcact agtacgtttt taagtagata
       61 tcaaaatgac taatttcttc actattaaaa atcttattat ctttatacct tttaaagaaa
      121 ctgtctcgac ttaaaagctt atcatcctta ctaaataaat caacttcata atgtttacta
      181 tctttagtag caaggaaaag gctccccgat gtataacgcg attccgaatc atagatattg
      241 tatttttcca ttagaaattt tcttatttta aaatcaaatt cctgtaaagt aaactgtttc
      301 ttttttatgc gaacagcctc agagggcaaa taagtttctt tcccatcttg atttaaaatt
      361 cctacaaact gtaatgattt atttccttga ttcgaatgtt ttacaatacc accataaata
      421 ttatctacat aaggtgggga aaaattataa gggagaccaa aaacatcgac cttatcccct
      481 tttataaact gatcagtaat gcctaaagat acaatagaat caacataaag aatacattcc
      541 gatctttttt gtttagtatt aatatgtatg ctatttgtag taacaaatgc aatttctaca
      601 ttttcataat cgcatggggt aatattatag gcaaacctta aacttctttt taaatctttt
      661 aaattttcat ctgcataagc taattgactt ccatagctaa aaacacatga taatatgata
      721 attgtcaaaa tgtttgtttt catttaaaac tttttatgtc ctgttttata cctcttaacc
      781 taaataaaaa tatcaatcgg tttcaacaac tgatgacact atagaaggaa gagattactt
      841 tagtaaagtc aacaaaaaaa gagcacacac ccgaaaatcg cttagggctg ctggattcct
      901 cccctgaccc acttcacgca caagtgttgc tccgcctatt attatatcat attacggaca
      961 gtttgcaagt tttccaaata agtaaaatca ctttcaagtg agcatagctt cattttggga
     1021 caagatggac tgaataccta gaaaaatcat gaccctttcc aaacacgatt ttgctttttg
     1081 aaaatctttc cctctcccca ttccagctat tctctatgtt ataataagat tattagatta
     1141 gaatgggagt tatgatgaaa caaaagatta aaatcttaac agtaattggc cttatgacag
     1201 ttggtatgtc tgcgtgtcac aacactagca aaccttctaa taccgattct gttttttctt
     1261 taaccggaaa aaagcgccaa caaatcgtca aacaagtaag acaacggtat tatttccaac
     1321 aattatccaa aaccgaacaa gaaaattacc tcactttata cgatagtcta gcacaatttc
     1381 gtgaaatcat cagtttgacg ccggcttcta aaaaaagctt aattaaaacg attgacgcct
     1441 ttgtgatgga taatcccgaa ttttactgga ttacatctgc cgattatcga tttgagtttt
     1501 ctgatcaaac ggtctttgtc acctttccta tcccagaaga tgccaagaac gtttatcaag
     1561 acttgcaagc aatcggtaat gatatcgtcg caaacatgcc atcaaaggat cgctatgagc
     1621 aggtcaaata tttttatgag gtcatcattc gagacacaga ctacaataaa aaagcctttg
     1681 aagcctatca atcaggcagt caagcccagg tcgcttctaa tcaagacatt aaaagtgttt
     1741 ttattgatca tttatctgtt tgtaatggct atgcccaagc ctttcagttt ctttgtcaaa
     1801 aagctggcat tccagtcgcc tatatccgtg gaactggtac atctcagcaa cctcagcaat
     1861 cttttgcaca tgcatggaat gctgttcaaa ttaataacac ttattatggc gttgatgtca
     1921 cttggggtga ccctgttttt gataaccatc tctcacatca aaagcaagga actatcaatt
     1981 acagttttct atgcctgcca gaccatctaa tggccttatc acatcagcca agcaaagaca
     2041 ttgcttttaa tactaaagaa cgttttgaaa atgtttggac tataccttct tgtacagatg
     2101 attctttgct ttattctaaa cgtcatcaaa gctatatctc aacgtttgac agtgacgcca
     2161 tccttgcaag ccttgaaaat cagttgttaa atagacaaga gccactgtct ctacaatttg
     2221 ctcatcaaga tgattatcaa caaatggtaa ctgatttaac gacgaatcaa acaggttatc
     2281 ataacctttt caatcagtat tggaataatt atacgggctt tacctatgga ctcttaccag
     2341 agactcttag tatttcattt gctagtagga actaggttta gacacatgaa acacaaagtt
     2401 aagatgatta tctttgtcaa acattacaag aaattgatat actaacacaa ttgaaggcga
     2461 ttattattct aaccgtcttc aatagatata accttttcag attgagcaca tctaatgctt
     2521 gtaacattag caacagtgat gagctctttt ttaatgcttt agcaaaatat atcagctaag
     2581 acaaaagatt aaataactca agccatgcct ataagtcact tgtttttaat ccctgtgtca
     2641 gtgtcaatct acgccacaga taactgacag ctctgagccc tagaagtcta agcttgataa
     2701 gtttactaaa aacaaaaata aggtgagcca ttactatctg tttttagata gtaaatcttt
     2761 ttaagaatga ctaactcatc tagtcatcac gtgttttatt tttcgattgt tttcatctcg
     2821 attgggtctg aaaaagagcg cttctagtac ttggaaacgc ccttttgtat attctatctt
     2881 cgtatgttga atctaacaac cattggaaga atggtctgac gtttcactca gtattttgac
     2941 aggctaccct tcaccaaaaa tgcatgtgat aacaaactcc ttaaaactct agtcaaaggg
     3001 atctgactgt tccttgatca aatgcttagc tatttttttg cgctctcgcc cttggcggaa
     3061 aaaggtttgc atgatgttag cacagtctgc tgctagcagg cctcgttcaa cttgcacgcg
     3121 atggtttaag cgctcatctg tcaaaatctg gtaaaggctg tctgctccac caaatttttg
     3181 attgctggct ccataaataa catgcggaat ccttgctagc ccaatagcac cactacacat
     3241 cacacaaggc tcgatggtca caaagagtgt ggtgtctaat aaacgccagt tgccttcatg
     3301 agcgttagcc tcgttaatag ccatcatttc agcgtgcata atggcttggt tactctcttc
     3361 acgcgcattg tgcccacgac caataatctc accatccttg acaatgacac agccaatggg
     3421 gatttcagct ttctgtaagg atttttcggc ttcttttaaa gcctcttgca tgaaataagt
     3481 ttgctcttct aaactatatg gcatcaagaa agactcccca agttccaaga tttgtgtggt
     3541 aaggcaaggt caacaggaat attacctgca taggcctgtg tttcttgcct caacacttct
     3601 aaatggtttt tggaattaat accttctgga ggaaccggtg gcatgtgggt caagacaagc
     3661 ttcttaacac ctgcctggct agccattagt cctgcttcct tactggtcaa atgggccata
     3721 tgattttcat tgccttcgta taagtaaaca tccgctaaga aaaggtctgc ttcttttgcg
     3781 aagtctgcca atccttcaaa ataaccactg tctccagtaa agacaagtac ctgaccagtc
     3841 gctctttcta caattcgaaa ggcataacac acaactggat gaactatttt aagaaaggta
     3901 atgtcaaaag gaccaatctg ttcaactcca gtaacatcat aggctctacc ttctgaaaca
     3961 tcaggaatgc ttaacttagc aaattcgtgt cggtcctcat catgcccata aatgggcaat
     4021 actttaggct gccacaggtg tttaggataa agttgaaaat aatggcgcaa cacgcctaaa
     4081 tcggccacgt ggtctggatg gtaatggcta ataatcactg catccaaatc gagaggactg
     4141 atttcttttt ccagctcatt gagcgctcga ctaccagcat ccatcaataa ctgaaagccg
     4201 tcatgtcctg tgaccaaata agaggttgtg cctgcatctt gatagggata ggctccccaa
     4261 gaccctaagg tggttaattt catctcaaac ccttctttct tgctttaact cttattataa
     4321 caaaaattag tgtcaaagag gattgagacc caccaaaaac aggtcctagc agataaatct
     4381 gccaagacct gttcaacaca acttaactat ttgatgatta gattactgtt ttgtattcaa
     4441 aaaactatct ttgtatttaa cagcgataat ggtcgcaatg attgctttga tggtatctcc
     4501 tggaataaag accaagtttg acaaaagcga tgtcacaaga gacatgccag tgtaggctgc
     4561 caaccagata gcacccagaa catcaaccag tacaactcct gagattaaaa gagctaggaa
     4621 tgctaatggc gtgctttttg agaaccagtt gcggtaaagt aatgaaaaga caataggcac
     4681 aagaaggtag gcaataacat agccagcaga tggccccatc aatactggaa tggttgttct
     4741 tgagcctgag aaaacaggta agaaaaggcc aatcactaaa aataagaaaa cagataaggt
     4801 tcccttttta ccacccagca tcaatccagc caacatgaca cctaagtttt gtaaaacaat
     4861 agggactggg ataaagccta gcgggatagc aggaataaag cctaaaataa taatcaaagt
     4921 ggtcatcata gccaccttaa caagttcttt ggttgtaaac atcagttacg actcctttaa
     4981 aaaatagata tccttgtgcc tatgacaggc tggattaaag ggactttgac aataaggaca
     5041 gcatccacaa ccatactcag ctaggctcaa gagctttcga caatgcccac aaataacagg
     5101 agatgtttct tgatgtcctg ttggcacaaa ggcatgatct gttaactggt catggcaatg
     5161 gtaacaagca taataagtct gacaaaaagc acatttgagc ccaacaatat caagcggggt
     5221 atgataatgt aggcaccgat actcttggtc aaggtcaatt ccaaaacagt ccgtcatatc
     5281 tcatcctctt gtaatagttt aatcaaaagc ttatgatttg tcaactctat ttttacaaaa
     5341 gggtaactgt tttctgaaaa agaacacgta aaacaagaac tttcctttaa aaagtgacct
     5401 atcagttgaa agcaatagga catccgtcta tacaaaaaaa cctgaaacca aagacgatag
     5461 atcgccctca gttccaggat catagcattt ttaattagaa attacggttt gatttattat
     5521 aaccataccg ttcaagaaaa tcttggcgga attcaagtaa gttatcgtcc ataatagcct
     5581 gacgtacttt tttcatcaag ttaaccaaga aataaagatt gtggtagctt gtcaaacgaa
     5641 taccaaaagt ttcgtccgct tttagcaagt gacgaatgta ggcacggcta tagttttgac
     5701 aagtgtagca gtcacagtca tgatcaagtg gggtaaaatc ttcagcgaat ttggcatttt
     5761 taaccacaag gcgcccctca ctagtcatac aggtcccatt acgggcaata cgcgttggca
     5821 agacacagtc aaacatatct acaccacgaa tcacaccatc aatcaagcta tctggtgctc
     5881 ccactcccat caggtaacga ggtttatttt ctggtaagag gggagtcgta aagtcaagga
     5941 cggcattcat ttcttcatgg ctctccccaa cagctaaacc accaatagaa taacctggga
     6001 aatccatagc caccaaatct gccgcagact gacgacgaag gtcctcaaat ccagctcctt
     6061 gcacaattcc aaataatcct tggtcatgag gacgacgatg ggcttttaag ccacgttctg
     6121 cccaacggct agtacgttca atggattttt tcacgtaatc ataaggttgg taaaattgcg
     6181 ggcactcgtc aaaactcatc atgatgtctg agccaaggtt attctgaatg gaaatggctt
     6241 tttcgggtga tagaaacatt tttgagccat tcaagtgatt tttgaaagtc actccttctt
     6301 cagtgatgtt tcgtgaatcg gctaaagagt aaacttgaaa accacccgaa tcggttaaga
     6361 ttggctgatc ccagttcata aacttgtgca aaccaccaga gcgagcaatc aactcatctc
     6421 ctggacgcaa ccagaggtga taagtgtttg ataaaataat ccctgatcca attgctttta
     6481 actcttctgg agactgtgtt ttaacagtag cctgagttcc cactggcata aacattggtg
     6541 tcggaaaggt tccgtgaggg gtgataattt cgcccgagcg tgcaccggtg tgcttttctg
     6601 ttttgataag gcgatactta atcggataat ctgtcatgtg ctttcttcct tgctatcaag
     6661 ataaacagtc ttgagctttt ttgatctgcc tgagtttcca tcataatgaa gggcttcaag
     6721 atgacatgtt gttctagcgc ctatcatttt atcaaaaaat ccataatttg tcatgctttc
     6781 ccaaaaaagt atagtcaaaa taatggttag ctgtctcatt ttgttttatg gtataatgaa
     6841 ataaaggagt ctgttatgtc aattaaagca atcaaaggcc aggctagaga cacgttaaaa
     6901 aatctctctg gcaagtattt actattttta attccaaccc ttctctttat gtttcatttc
     6961 ggtattgaga ttcatcaagg ttacgtccta tctagtggta ttgaagtctc gctagcggct
     7021 agttatttcc cattactact aggacttatc cttagccttt tcattttatc agcatccttt
     7081 acaatgattg atgttgttag gcattttcgt cagaaagttt cttttgccga atcaacaaca
     7141 gccttctcaa aagaattctt tgggaacctg ctggttttag cgatcactaa gtggctattc
     7201 tttctcatct ggtcactaat ttggtttttt ggcctcttta ttttcctttc cggactttca
     7261 gcgtttttgg tcaatgctaa atcaggtagc tcaacagtca tttcactgat ctttttacta
     7321 tttggagcag ttttgtcctt aattggtttt ggtatttata tcaaccgtta ttacgcttat
     7381 agtctatcag aataccttct ctacgataag gttaaagagg ggacttatct aggagctata
     7441 gccgtcattg aaacaagtgt agctatgatg aaaggttata aatggaagct ttttttccta
     7501 caacttagct tcaccggttg gtttctactg aatattgtta cttttgggtt acttaatatc
     7561 tatcttttac catattttac aacagccaat gtcatttttt atgatcaact caagaaacgt
     7621 ttcaaagaca aagatgaccc aattgaagga gaacatttaa gccttcatca gcttaataaa
     7681 aagcctactc ctatgatgta tgacgatcaa aaagactaaa taaatcctcc atccataaaa
     7741 atagcccttg aactgcaccc caaaagttag acacaaaatc taacgattgg ggtgctattt
     7801 ttttatgaaa tttaatcaag aaacgaaagt taagatttat gagttacggc aaatgggaga
     7861 gtccattaaa tccatatcaa aaaagtttga tatggcagaa tccgatctca aatatatgat
     7921 tcgcttgatt gataggtatg gggtaaccat tgttcaaaaa tgtaagaata attattattc
     7981 tccagaactg aagcaagaga taataaatga agtcctgatt gacggccaat ctcagaaaca
     8041 aacatcctta gattatgctt taccaacttc tagtatgctc tcaaggtgga tagcgcaata
     8101 caagaaaaac ggttatacta ttcttgaaaa accaagaggg agaccgagta agatggggcg
     8161 taaacgtaag aaaaatttgg aagagatgac agaggttgaa cgtcttcaga aggaattaga
     8221 ataccttaga gcggagaatg ctgtgctaaa aaagctgaga gaataccgct tgagggacga
     8281 agtaaagctc aaagagcaac agaaatcatt caagcattaa ggaatcaatt tcccttagag
     8341 atgctacttg agatccttga tttatcacgc tccacctatt actatcaagt gaagcgacta
     8401 gctcaaggag ataaggatat agaactaaag catgtgattc gagagattta tgatgagcat
     8461 aaaggtaatt acggttatcg tcgtattcat atggagttgc gtaaccgagg atttgtggtc
     8521 aatcataaga aagtt
//
LOCUS       DAUGFP010000055         8021 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.055, whole genome shotgun sequence.
ACCESSION   DAUGFP010000055 DAUGFP010000000
VERSION     DAUGFP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.055"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(36..821)
                     /locus_tag="VH663_001435"
     CDS             complement(36..821)
                     /locus_tag="VH663_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174623.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNINELLEGFFNSFKMDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHSHIFNIN
                     TSADSFPFTEQKTIYLLILEQFIEMIMFYLATNFIIKNKLNLQ"
     gene            complement(831..1529)
                     /locus_tag="VH663_001436"
     CDS             complement(831..1529)
                     /locus_tag="VH663_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174624.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            complement(1529..1900)
                     /locus_tag="VH663_001437"
     CDS             complement(1529..1900)
                     /locus_tag="VH663_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ8174625.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            2085..5195
                     /locus_tag="VH663_001438"
     CDS             2085..5195
                     /locus_tag="VH663_001438"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEQ8174626.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELTDCQQLTTFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQELTEAGLKEKGLWKEPYQSRLLHELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQVYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSDYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIIEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKIMDNLDGLLVFVNELGSLFSDSSFSWVDTKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            5275..6288
                     /gene="pfkA"
                     /locus_tag="VH663_001439"
     CDS             5275..6288
                     /gene="pfkA"
                     /locus_tag="VH663_001439"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEQ8174627.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            6351..7853
                     /gene="pyk"
                     /locus_tag="VH663_001440"
     CDS             6351..7853
                     /gene="pyk"
                     /locus_tag="VH663_001440"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEQ8174628.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICRETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
BASE COUNT         2545 a         1342 c         1658 g         2476 t
ORIGIN      
        1 tgtgtcatag tttaaaagaa agtcttaacg cttacttact gtaggttgag tttatttttg
       61 ataataaaat tagtggcgag gtaaaacatt atcatttcaa taaattgttc caaaataaga
      121 agataaattg ttttttgctc agtaaatggg aaactatcgg cacttgtatt aatattaaag
      181 atgtgactgt gaacatatgt tgcagcaacg ctaatcagaa taactaatat aaaatatgca
      241 ataaaggcca tcaagcctcg ccgattagaa aaaagttgac ctatagaaat ggaaagataa
      301 attaataaga tactcgtaaa tgttgataat aggacatagg ctagtacagt tagcatattg
      361 atgaaataat ccatcttaaa actattaaag aatccttcta atagttcgtt gatgttaaac
      421 attggtacaa ttacaatagc gatagcaaat gcaaggatga tagtattgaa aacactgcaa
      481 ataaaagaag ctagtagttt tgataaaata atttgatgcg aattaacggg taaagtcaac
      541 gttaaatatc cttcccgtcc aaaaacactt ttgctaaaac gtttgataat gataagtaat
      601 gttgataaga gggaaccagc gatcagggag ccaaaagtta aacctaatgt cagaggcaac
      661 atcttattgg ttagtactcc aaatagtcca tcactattgc tttgagcaaa cagttttatc
      721 gtaaatgaca aaatggcagc gatggcaata acgaaagcat taagcgcaaa gtaccatttt
      781 ccaatagatc taaattcgta ttttaataat tttccaaaca tgatatcctc ctaaacctta
      841 taagtttccc taaagaggga atcaatggat tgctggtatt cttgtcttag atcatcagca
      901 ttgccagata agaaaagtct tccttgtttc aagaaaatga cttcgtcaag gataggttca
      961 atgtccgaaa tcaagtgggt tgagataata acagaagcat tttcacaata actattaatg
     1021 atggttttta ggatgtagtc tcttgcggca gggtcaactc caccaatagg ttcatcaagt
     1081 acatagagtc tggctttacg actcatcaca agaattaatt gtactttttc tttgtttcct
     1141 ttggatagag ttttgaagcg gtcttcaggg tctagttcta aatctcttag aaggcttgtt
     1201 gctttggatt tgtcaaagtc gctgtaaaaa tcactaaaga actccaacat gtccttaatt
     1261 ctcatatttt cattgagata agtagtatct ggtaaataag aaataatttt ttttgtctca
     1321 acacagggac ggtagccatc aatgacaatt tctcctttat ttggttgtaa aaggccatta
     1381 atgagtttaa ttaaagttgt tttaccactt ccatttggac ctaataatcc gataatttta
     1441 ccgttcggga tagtaatggt taagtcatca atagcttttt tttcacggta agattttgaa
     1501 acatgatgta gttgcaatag gtgtgccatt attgtgaatc ctcctttaaa aagctcgtca
     1561 aaacagggat aatctcagtg tgactaaatc ccatttttgt catattggtg ataaatgatt
     1621 ctaattcact aattgctaac tctcgtcttt tacgagcaat taatttttga tcgtcagtta
     1681 caaaacggcc agcagtacgt tgcgaataaa ccattccttc ccgttcaagt tctgtaaacg
     1741 ctcgttgcat ggtgttagga ttgacacctg cgatttctgc atattcacga acagttggaa
     1801 gttgatcacc actcttgatt tcttggctaa taatttgcat catgacatgt tgtgctatct
     1861 gcgcataaat aggagatttt tcatcaaatt tccaagacat gtggtctcct ttctgagtaa
     1921 tatagatatt tgtactaatt aaatggtaca gaaaaaaaag gggaatgtca agttttatgc
     1981 catttttcac tctttgagaa gtagacgtag attagagaga aaattgaagc aagaaaacct
     2041 ttttcatagt ataataggga acaagataac caaaggagga acttatgttt gctcaacttg
     2101 atactaaaac tgtatactca tttatggata gtttaattga cttaaatcat tattttgaac
     2161 gagcaaagca atttggttac cacaccatag gaatcatgga taaggataat ctttatggtg
     2221 cttaccattt tattaaaggt tgtcaaaaaa atggactgca gccagtttta ggtttggaag
     2281 tagagattct ctatcaagag cggcaggtgc tccttaactt aatcgcccag aatacacaag
     2341 gctatcatca gcttttaaaa atttccacgg caaaaatgtc tggcaagctt catatggatt
     2401 acctctgcca acatttggaa gggatagcgg ttattattcc tagtaagggt tggagcgata
     2461 cattagtggt cccttttgac tactatatcg gtgttgatca gtatactgat ttatctcata
     2521 tggattctaa gaggcagctt atacccctaa ggacagttcg ttattttgcg caagatgata
     2581 tggaaaccct gcacatgttg catgccattc gagataacct cagtctggca gagacccctg
     2641 tggtagaaag tgatcaagag ttaacagatt gtcaacaact aaccaccttc tatcaaacac
     2701 actgccctca agctctacag aatttagaag acttagtgtc aggaatctat tatgatttcg
     2761 atacaaattt aaaattgcct cattttaata gagataagtc tgccaagcaa gaattgcaag
     2821 agttgactga ggctggtttg aaggaaaaag gattgtggaa agagccttat caatcgcgct
     2881 tactgcatga attggtcatt atttctgaca tgggctttga tgattatttt ttgattgtgt
     2941 gggatttact tcgctttgga cgcagtaaag gctattatat gggtatggga cgtggctcgg
     3001 cggcaggtag tctagtggct tatgctctga acattacagg gattgatcca gttcaacatg
     3061 atttgctatt tgagcgcttt ttaaacaaag aacgttatag catgcctgat attgatatcg
     3121 atcttccaga tatttaccgt tcagaatttc tacggtatgt ccgaaatcgt tatggtagcg
     3181 accattcggc gcaaattgtg accttttcaa cctttggacc taaacaggct attcgtgatg
     3241 ttttcaaacg attcggggtt ccagaatacg aactgaccaa tctcactaaa aaaattggtt
     3301 ttaaagatag cttggctact gtctatgaaa agtcaatctc ttttaggcag gttattaata
     3361 gtagaactga atttcaaaag gcttttgcca ttgccaagcg tatcgaagga aatccaagac
     3421 aaacgtccat tcatgcagct ggtattgtga tgagtgatga taccttgacc aatcatattc
     3481 ctctaaaatc gggcgatgac atgatgatca cccagtatga tgctcatgcg gtcgaagcta
     3541 atggcctgtt aaaaatggat tttttggggt taagaaattt gacctttgtt caaaaaatgc
     3601 aagagaaggt tgctaaagac tacgggtgtc agattgatat tgcagccatt gatttagaag
     3661 acccgcaaac gttggcactt tttgctaaag gggataccaa gggaattttc caatttgaac
     3721 aaaatggtgc tattaatctt ttaaaacgga ttaagccaca acgttttgaa gaaattgttg
     3781 ccactaccag tctaaataga ccaggggcaa gtgactatac cactaatttc attaaacgaa
     3841 gagaaggaca agaaaaaatt gatttgattg atcctgtgat tgctcccatt ttagagccaa
     3901 cttacggtat tatgctttat caagaacaag ttatgcagat tgcacaggtt tatgctggtt
     3961 ttacgttagg caaggccgac ttgttaaggc gtgccatgtc taaaaaaaat ctacaagaaa
     4021 tgcaaaaaat ggaagaagac tttattgctt ctgctaagca cctagggaga gctgaagaaa
     4081 cagctagagg actttttaaa cggatggaaa aatttgcagg ttatggtttt aaccgcagcc
     4141 atgcctttgc ctattcagct ttagcttttc aattggctta tttcaaagcc cattacccgg
     4201 ctgtttttta cgatatcatg atgaattatt ctagcagtga ctatatcaca gatgctctag
     4261 aatcagattt tcaagtagcg caagttacca ttaatagtat tccttacact gataaaattg
     4321 aagctagcaa gatttacatg gggctgaaaa atattaaggg gttgccaagg gattttgctt
     4381 attggattat tgagcaaaga ccatttaata gcgtagagga ttttctcact agaactccag
     4441 aaaaatatca aaaaaaggtt ttccttgagc ctctgataaa aataggtctg tttgattgct
     4501 ttgagcctaa ccgtaaaaaa attatggaca atttggatgg tttactggta tttgttaatg
     4561 agcttggttc tcttttttca gattcgtcct ttagttgggt agatacgaaa gattactcag
     4621 caactgaaaa atattctttg gaacaggaga tcgttggggt tggcatgagc aagcatcctt
     4681 taattgatat tgctgagaaa agtacccaaa cttttactcc catttcacag ttagtcaaag
     4741 aaagcgaagc agtcgtactg attcaaatag atagcattag gatcattaga accaaaacaa
     4801 gtgggcagca aatggctttt ttaagtgtga atgacactaa gaaaaagctc gatgtcacac
     4861 tttttccaca agagtatgcc atttataaag accaattaaa agaaggagaa ttctattact
     4921 taaaaggtag aataaaagaa agagaccatc gactgcagat ggtgtgtcag caagtgcaaa
     4981 tggctattag tcaaaaatat tggttattag ttgaaaacca tcagtttgat tcccaaattt
     5041 ctgagatttt aggtgccttt ccaggaacga ctccagttgt tattcactat caaaaaaata
     5101 aggaaacaat tgcattaact aagattcagg ttcatgtaac agagaattta aaggaaaaac
     5161 ttcgtccttt tgttctgaaa acggtttttc gataaaaatg gacaattagt gaagtatttt
     5221 gcaaatgaat gtgctataat aagaccgtta aattaattaa aggagtacca gaaaatgaaa
     5281 cgtattgctg ttttaaccag tggcggcgac gctcctggta tgaatgctgc tattcgtgca
     5341 gtcgttcgta aagcaatttc agaaggaatg gaagtttatg gtatcaaccg tggctatgca
     5401 ggtatggttg atggcgacat tttcccactg ggttcaaaag aagttggtga taaaatctca
     5461 cgtggtggaa ctttcttata ctcagctcgt tatccagaat ttgctcaact tgaaggtcaa
     5521 ttggctggta ttgagcaact taaaaaacat ggtattgagg gtgttgttgt tattggtggg
     5581 gatggttctt atcatggcgc tatgcgcttg acagagcacg gcttcccagc ggtaggtatc
     5641 ccaggaacaa tcgataacga tattgcggga actgactaca ctatcggatt tgatacagca
     5701 gttaacacag cagttgaagc tattgataaa cttcgtgaca cctcatcaag tcacggacgt
     5761 acttttgtgg ttgaagtgat gggtcgcaat gcgggtgata ttgctctttg ggcaggtatt
     5821 gctagtggtg ctgatcaaat cattgttcca gaagaagagt ttgatattga aaaagtggca
     5881 tcaacaatcc aatatgattt tgaacacaaa ggtaaaaatc accacattat cgtcttagct
     5941 gaaggtgtta tgagcggtga agcttttgct caaaaactga aagaagctgg cgataagagt
     6001 gaccttcgtg tgacaaatct tggtcacatt cttcgtgggg gttcaccaac tgctcgtgac
     6061 cgtgtcattg cttcttggat gggttcacat gctgttgagc tacttaaaga aggcaaaggt
     6121 ggtttggctg ttggtattca caatgaagaa cttgttgaaa gcccaatcct tggtactgca
     6181 gaagaaggtg ctttgttcag cttgactgaa gaaggcaaga ttattgttaa taatccacac
     6241 aaagctcgtt tagactttgc agctttgaat cgttcattgt cacaataagt ttagttaaaa
     6301 ttgttaaatc gtcaattatt gacaaaaaaa taagggagtt tcagagtaaa atgaataaac
     6361 gcgtaaaaat cgttgcaaca cttggtcctg cggttgaaat ccgtggtggt aaaaaatacg
     6421 gagaagatgg ctactgggct ggtcaacttg atgttgaaga atcagcaaaa aaaatcgctg
     6481 aattaattga agctggtgcg aatgttttcc gtttcaattt ctcacatggt gaccacaaag
     6541 aacaaggtga ccgtatggca actgttcgcc ttgcagaaga aattgctcgt caaaaagttg
     6601 gtttcctttt ggatactaaa ggtcctgaaa tgcgtacgga attgtttgca gatgatgcta
     6661 aagagtttag ctatgttact ggagaaaaaa tccgtgtagc gactacacaa ggtattcaat
     6721 caactcgcga tgttattgct ttgaacgttg ctggtagcct tgacatctac gatgaagtag
     6781 aagtaggtca tactattctt atcgatgatg gtaaacttgg tttgaaagtg atcgacaaag
     6841 atatcgcaac acgtcaattt atcgttgaag tagaaaacga cggtattatt gctaaacaaa
     6901 aaggtgttaa tatccctaac actaaaattc ctttcccagc acttgcggaa cgcgacaatg
     6961 ctgatatccg ttttggtctt gaacaaggac ttaactttat tgcaatctca tttgttcgta
     7021 cagccaaaga cgttgaggaa gttcgtgaaa tctgtcgcga aactggtaac gaccatgtgc
     7081 aattatttgc taagattgaa aaccaacaag gtatcgataa tcttgatgaa atcatcgaag
     7141 ctgctgatgg tatcatgatt gcacgtggcg acatgggtat cgaagtacca tttgaaatgg
     7201 ttccagtttt ccaaaaaatg attatcacta aagtgaatgc agctggtaaa gcagttatca
     7261 cagcaacaaa catgcttgaa acaatgactg aaaaaccacg tgcgacacgt tctgaagtat
     7321 ctgacgtctt caatgctgtt atcgatggta ctgatgcaac aatgctttca ggagagtcag
     7381 ctaacggtaa atacccggtt gaatctgttc gtacaatggc gactattgat cgtaatgcac
     7441 aaactcttct taacgaatat ggtcgtttag attcatctgc cttcccacgt accaataaaa
     7501 cagatgttat tgcttcagct gttaaagatg ccactcattc aatggacatc aaacttgttg
     7561 tgactattac tgagacaggt aatacagctc gtgcgatttc taaattccgt ccagatgcgg
     7621 atatcttagc tgtaacattt gatgaaaaag tacaacgtgc tttgatgatt aactggggtg
     7681 ttattcctgt tcttgctgaa aaacctgcat ctactgatga catgtttgaa gtagcagagc
     7741 gtgttgctgt ggaggcaggc ttagttcaat ctggggataa tattgttatt gttgctggtg
     7801 ttccagttgg tactggtgga acgaatacta tgcgtgttcg tacagtaaaa taaatgtgtt
     7861 ataactattt acctaaaggt tagaaggcaa gcttctaacc ttttttgata tcaaaaatat
     7921 catgttaatt cactagtgtt aatttacttt aatgagtcta agtttgtatt ttatacagaa
     7981 tgagcaacgt ttattggtat tgatcaaaaa ttgtttactt t
//
LOCUS       DAUGFP010000056         7405 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.056, whole genome shotgun sequence.
ACCESSION   DAUGFP010000056 DAUGFP010000000
VERSION     DAUGFP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.056"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..320
                     /locus_tag="VH663_001441"
     CDS             <1..320
                     /locus_tag="VH663_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEQ8174629.1"
                     /translation="VYISNASKITINIVEKMGIIFVISAIIGATVLAMYLTWTPVGSS
                     TVLGVQSRYLIGIIPLVLLLFSSQQQKFKQIEDILSDKLAIHISLLFILAMLMSTIFR
                     YYH"
     gene            414..1637
                     /gene="pepT"
                     /locus_tag="VH663_001442"
     CDS             414..1637
                     /gene="pepT"
                     /locus_tag="VH663_001442"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEQ8174630.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALQLAIDFHEKLPAKDRPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            1678..2166
                     /locus_tag="VH663_001443"
     CDS             1678..2166
                     /locus_tag="VH663_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEQ8174631.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHVEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(2153..2350)
                     /locus_tag="VH663_001444"
     CDS             complement(2153..2350)
                     /locus_tag="VH663_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEQ8174632.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            2399..2875
                     /locus_tag="VH663_001445"
     CDS             2399..2875
                     /locus_tag="VH663_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ8174633.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKTSSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            2890..3570
                     /locus_tag="VH663_001446"
     CDS             2890..3570
                     /locus_tag="VH663_001446"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEQ8174634.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPISFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWV
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    3594..3717
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            3732..4262
                     /gene="infC"
                     /locus_tag="VH663_001447"
     CDS             3732..4262
                     /gene="infC"
                     /locus_tag="VH663_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEQ8174635.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            4304..4501
                     /gene="rpmI"
                     /locus_tag="VH663_001448"
     CDS             4304..4501
                     /gene="rpmI"
                     /locus_tag="VH663_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEQ8174636.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            4560..4919
                     /gene="rplT"
                     /locus_tag="VH663_001449"
     CDS             4560..4919
                     /gene="rplT"
                     /locus_tag="VH663_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEQ8174637.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(5210..7381)
                     /locus_tag="VH663_001450"
     CDS             complement(5210..7381)
                     /locus_tag="VH663_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEQ8174638.1"
                     /translation="MKKFKTLITGFINTRLGFIITLLFCYWIKTLWAYHTDFSLDLGN
                     IYQVFLTIINPIPLAFLLLGVALYVKNTRAFYICSWVVYIILNILLISNSIYYREFSD
                     FITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSITKKIKNDQRPFNKR
                     AAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPAFTLYSGNQTYQAQ
                     KERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLESFQQFLIDYKLKEG
                     DKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSLFGLNSGSFMVNYG
                     GENTQFATPSILAQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYFFDSSYFSKQNSKN
                     SFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGESSEEGFPLAKTDD
                     ETINGYFATANYLDAALKSFFDYLKATGLYDNSIFVLYGDHYGISNSRNSSLAPLLGK
                     DSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLLHILGIDTSQFVQL
                     GQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNPDEMTIAKTKEIRS
                     AVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKDISAKLGSESTSLY
                     SKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNTKNRE"
BASE COUNT         2419 a         1209 c         1470 g         2307 t
ORIGIN      
        1 ttgtttatat tagtaatgct agtaaaataa ctattaatat tgtagaaaaa atgggtataa
       61 tctttgttat ttcagcgatt ataggtgcaa ctgtattagc tatgtacctg acttggacac
      121 cagtagggtc ctcaactgta ttaggtgttc aaagtcgtta tctgatagga attatcccat
      181 tagtgttgct tctttttagt agtcaacagc aaaagttcaa acaaatagag gatatccttt
      241 ctgacaaact ggctattcat atttcgcttt tgtttattct cgcaatgtta atgagtacta
      301 tttttagata ttatcattaa actcttaatt agaggcttta aaaaacttag cttgaataag
      361 tattttaaag ccttttatgc tataattaaa ttgagaaatt attgaggtgg cctatgaagt
      421 acgataactt gttagatcga tttataaaat atgtcaaagt gaatacaaga agcgatccag
      481 acagtgagac tactcctagt acggagagtc aagaagcatt tgccttaacg attttaaagc
      541 ctgaaatgga ggcaattggt ttacaagatg ttcattataa tccagttaat ggctatctta
      601 ttggaactct gcctgccaat aatccaacct tgacgcgtaa aattggtttt atagcccata
      661 tggatacggc tgatttcaat gctgaaaatg ttaatccgca gattattgac aattatcaag
      721 gaggggacat tacacttggc agttctaatt ataagttgga tccaaaagca tttccaaatc
      781 ttaataatta tatcggtcaa acattgatta ccactgatgg gactacactt ttaggggcag
      841 atgataaatc tggtattgct gaaatcatga cagcaataga attcttaaca tcacaaccac
      901 aaatagagca ctgtgacatt aaagtggctt ttggccctga tgaagagatt ggggtcggag
      961 ctgataagtt tgaggtggct gattttgagg tggattttgc ttatactatg gatggcggtc
     1021 ctttaggcga attgcaatat gaaactttca gcgctgcagc tctagaagtg acttttttag
     1081 gcagaaatgt tcatcctggt acggctaagg atcaaatgat taatgccctt cagttagcaa
     1141 tagattttca tgagaagcta cctgctaaag atcggcctga atacacagat ggttatcaag
     1201 gtttctatca cttaacggga ctaactggaa cagttgaaga ggcacgcgca tcttacatta
     1261 ttcgtgattt tgaagaagca tcatttgaag ctagaaaagt gaaagttgaa aatatagctc
     1321 aatcgatgaa tgctcagcta ggaacaaaac gtgtcttggt agagcttaat gaccaatact
     1381 acaatatgaa aaaagttatc gaaaaagata tgaccgctat tgagttagct aaagaagtaa
     1441 tggaagagct tgctatcaag cctgtaatcg aacccatacg tggaggaaca gatggctcta
     1501 aaatttcatt tatgggtatt cccactccta atatttttgc agggggtgag aatatgcacg
     1561 ggcgttttga gtttgttagc ttacaaacga tggagagagc tgtcgatgtc attattggac
     1621 ttgtttgcaa agcctaatta tcatggaatt gatatttagt ggaaggggct tataaaaatg
     1681 attaaactat ttggtaaaat aaggtatcac tggcagccag agttgtcttg gtctattatt
     1741 tattggtcta ttgcgtttgc acctattttt gtaggtttat ctttgttgta tgagcgcact
     1801 gaaataccta gtcgggtttt tattctattt gctatttttg ctgtcctagt tggcattgga
     1861 ttacatcgtt atttcattat tgaaaataat ggtattttaa gaatcgtatc ttttaaattg
     1921 tttggtccac gtaagctact tatttccact attactaaga ttgaggtgac caaatcaaca
     1981 ctttgtctgc acgttgaaga taaaagttat ctcttttata tgcgcaaatg gcctaaaaaa
     2041 tattttcttg atgccttagc ggttaatcca tacttccaag gagaagttat attaagtgat
     2101 aactttatta aattagacta ttttgaagtt tatcagcatg ataaaaaagc ccttactcga
     2161 gggtaagagc tttagttggg caagatttga cagctaaaat agcgtcctta tctgaaggac
     2221 aaattgactg acttgtttca gatgagcttg aaaatgtgac gataccattg tcatgataat
     2281 caaaaagtga tgaataggtt tggcatagac cacaagcgat gcatttttct ggaatgatag
     2341 atactttcat aactattatt ctaatatgat tttgttaaat taacaagggg ggacgatcat
     2401 ggctaaagaa ccatgggaag aaaaaattgt tgatgatact atagggacac gaacacgtaa
     2461 atcaagaaat gctttcatta gcacgccttg gttgactgct ttattaagtg tattctttgt
     2521 catcattgtt gctatacttt ttattttctt ctatacatca aatagcggta gtaatagaca
     2581 agctgaaaca aatggttttt atggagcatc cactcataaa aaaacaagga aagcttctaa
     2641 cgctaaaaaa acatcaagta gttcaacaac tacagacaca acaccttcta gcgaagaaac
     2701 acttgcttct agtgaaggaa ctggcgaaac ccttactgta ttggcaggtg agggggcagc
     2761 ttctattgca gctcgtgcag gtatttctgt ggagcagtta caagcactta atccagagca
     2821 catgactcaa ggatattggt atgccaatcc aggagatcaa gtcactatta aataattgaa
     2881 gaggaaaaaa tgaaagcaat taaaattgcg attgatgggc cagcttcaag tggaaaaagt
     2941 acagtagcca agattattgc aaaaaatctt ggctatactt atctagatac tggtgctatg
     3001 tatcgctcag caacttatat cgcactgaca catggctata cgggtaaaga agtagccctt
     3061 attttagagg aacttgagaa aaatcctatt tcctttaaaa aagcaaaaga tggctcccaa
     3121 ttagtttttc ttggggatga ggacgtgacc ttagccatta ggcaaaatga tgtgactaac
     3181 aatgtttctt gggtctcagc tttgccagaa attagagaag agctggttca tcaacaaaga
     3241 cgcattgcac aagctggtgg cattatcatg gatggtcgtg acattggaac ggttgttctc
     3301 ccagatgcgg aactaaaaat ctttttagtg gcttctgtcg aagagcgggc agagcgccgc
     3361 tataaagaaa acctagaaaa aggaattgaa tcagattttg agacgctaaa agaagagatt
     3421 gcagctcgtg attacaagga tagtcatcgt aaggtttcac ctctaaaagc agctgaagat
     3481 gcccttattt ttgataccac aggtgtttct attgatggcg tggtgcaatt tatccaagaa
     3541 aaagcagaaa aaatagttga catgtcctag agcctatgtt agaatagtaa caatgagaaa
     3601 agtagaagtg agagcttctc gccttgcgac ttaggttgtc tggccctaac atgtcaaatt
     3661 cctatggaat attatatgtg cgggcttgat ttatcaagtc cgctttcgtt tttctctaaa
     3721 aaaataaaga ggtgaagatc atagctaaaa aggatctatt cattaatgat gaaattcgcg
     3781 ttcgcgaagt tcgtctcgtt ggtcttgaag gtgaacaatt aggtattaaa ccattgtctg
     3841 aagcgcaatc attggctgat gcttcaaatg ttgatttggt tttaatccag ccacaagctg
     3901 ttcctcctgt tgccaaactt atggactatg gaaagttcaa atttgagtat caaaagaaac
     3961 agaaagaaca acgtaaaaaa caaagcgttg tgactgtgaa agaagttcgt ctcagtccag
     4021 ttatcgataa aggtgacttt gaaacaaaac ttcgtaacgg tcgtaaattc cttgaaaaag
     4081 gaaataaagt taaggtttct attcgtttca aaggacgtat gattactcat aaagagattg
     4141 gtgccaaggt actagctgac tttgctgaag cgactcaaga tattgctatc attgagcaac
     4201 gagcaaaaat ggatggacgc caaatgttta tgcaacttgc accaatttca gacaagaagt
     4261 aattgtcaca gtttcattat atctttaaga ggagaatact aaaatgccaa aacaaaaaac
     4321 acaccgcgca tcagctaaac gttttaaacg tacaggttca ggcggtttga aacgcttccg
     4381 cgcctttaca tcacaccgtt tccacggaaa aactaaaaaa caacgtcgtc atcttcgtaa
     4441 agctggtttg gttagctcag gagatttcaa acgtatcaaa gcaatggtta ctggtcttta
     4501 attttaaagg cagtagacat ttacgagtat tataagaatt atttgaagga gaactattaa
     4561 tggcacgtgt taaaggtggc gttgtttcac gcaaacgtcg taaacgtatt ttaaaattag
     4621 ctaaaggtta ctatggagca aaacacatct tgttccgtac tgcaaaagag caagtgatga
     4681 actcttacta ctatgcatac cgtgatcgcc gtcagaaaaa acgtgatttc cgtaaactat
     4741 ggatcactcg tatcaatgcg gctgctcgta tgaacggttt gtcatactca caattgatgc
     4801 acggtttgaa acttgctgaa atcgaagtaa accgtaaaat gcttgctgat ttagcagtag
     4861 ctgatgcagc agctttcaca gctcttgctg atgcagccaa agcaaaactt ggcaaataag
     4921 aaacgctata cctaaaaaat aactttctat tacctataga aagttataaa gaaacaaaaa
     4981 tgaaggagac gatggacgtc ttctttttat tatactcaat ataataaaaa gaagtttccc
     5041 atgttttatt accagtaatg tgggatattt agatggtagc aagaagtttt atagttgatt
     5101 tgttttcttt aggtctaatt agcatatttt gattactgat aaacttgaat atcgccaata
     5161 aaaaagaagc aaaattatta ttttgcttct agtcagatca ttaaattgat tattctctat
     5221 ttttggtgtt atcctctttt agttcagaag ttgccgcgtc agcttctacg ggatttagtt
     5281 ctaagtaaga tggtgcttta aaaagtttct gagttgattt atgaccattt ttactgtata
     5341 aacttgttga ctctgatccg agttttgctg aaatatcttt cagttgtttt aactgcttag
     5401 tgtagataaa ctggttagga tcaatagctt ttagaccatt ttgagtatca aagcgcagga
     5461 gatcacccgt ttgaatagcg tcgctagctg ctagttgttg agcaacagca gagcgaattt
     5521 ccttagtttt ggctatagtc atttcatcgg gattagtgat ttctaaacct gtttgcgtgt
     5581 tgtacagacg tccactatag ttagtgtatt caggagtcat ataagtccca gatgttcgtt
     5641 gtgccacaat ctggctattt tgaggtgata ataaatcttg tcctaattga acaaactgac
     5701 tagtgtcaat accaagtatg tggagtaaag taggaagagc atcgatttca ccgccaaagg
     5761 tttctttgat actaccattc gtataacctg gaatatggat catatacggg actcgttgta
     5821 gcatagcatt atcatattca gaccaagttt cagaatcttt accaagaagt ggagcaagac
     5881 tagaattacg agaatttgaa ataccataat ggtcaccata taaaacaaaa atagaattgt
     5941 cgtacaaacc agtggctttc aagtaatcaa aaaaagattt aagggcagcg tctaaataat
     6001 tggcagtagc aaagtaacca ttgatcgttt catcgtctgt tttggctaaa ggaaaacctt
     6061 cttcactact ttcgcctttt agactagtat aaggatagtg attactcaca gtgataaact
     6121 tggtataaaa aggttgttgc atctgttcaa gatattttat ggaatcctta aacatgtact
     6181 tatcatttaa cccatattga aaagagttct tactattttg tttagagaag tagctagaat
     6241 caaaaaaata attatagccc cattgtttat aagcattatt gcgattccag aaagttccaa
     6301 cgttaccatg aaagacagcg ctggtatagc cacctttttg ggctaaaata cttggagtag
     6361 caaattgtgt attttcacca ccgtagttca ccataaaaga accactattt aaaccaaata
     6421 aggaattctc catcatggtt tcagcatcag aagttttacc tgctttaacc tgatgaaaaa
     6481 agttagggaa tgctaaggta gcatttgagt gataaagcga gttgataaaa ggcgttacct
     6541 catattcttt atctccttct tttagtttat aatctattaa gaattgttga aagctttcta
     6601 aatgaagaac aataatattt ttgccttttc caattccaaa atactgggga tcaggtgctg
     6661 cataatgtcc tttgacataa gttttaacat caattaattc ttctgcagta gctccatttc
     6721 gttctttttg agcttgatag gtttgattgc cgctatagag ggtaaacgct ggtaatccta
     6781 aagctctcac aatataagta tttgaaaagc cacgtgttaa tagttccgga cgatcaattt
     6841 ctgctaaaaa aagattaata gaaagcaata gactagataa agcggtgatg gcaaatgctg
     6901 cgcgcttgtt aaaaggacgt tgatcatttt tgattttttt agtgatagat aaactaatta
     6961 gaataataaa atcaagaata tagataatat cccagatgcg taaaaggttt aaagcagagt
     7021 cccctaatcc agcggagact ttactgctag ctagcatggc actcactgta ataaagtctg
     7081 aaaattctcg gtaatatata gaatttgata tcaagagaat gttaaggata atataaacca
     7141 cccaagaaca gatataaaag gctcgggtat tcttaacgta aagagcgacc cccaaaagta
     7201 aaaaagctag tgggattgga ttgataattg ttagaaagac ttggtatata tttcccaaat
     7261 ctagtgaaaa gtctgtatga tatgcccaca gagtctttat ccagtaacaa aagagtagag
     7321 taattataaa acccaatctt gtgttgataa aacctgtgat aagtgtttta aatttcttca
     7381 cagtagcaac ttccttatcg ttttt
//
LOCUS       DAUGFP010000057         7312 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.057, whole genome shotgun sequence.
ACCESSION   DAUGFP010000057 DAUGFP010000000
VERSION     DAUGFP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.057"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            237..554
                     /locus_tag="VH663_001451"
     CDS             237..554
                     /locus_tag="VH663_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEQ8174639.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRTKVNQLAFVVENHFDIDDVFWHNIEFHYLVSPLEDPKLEMIENASDRFVNGFQSIN
                     WIK"
     gene            678..2576
                     /gene="mnmG"
                     /locus_tag="VH663_001452"
     CDS             678..2576
                     /gene="mnmG"
                     /locus_tag="VH663_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEQ8174640.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDADYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            2666..4642
                     /locus_tag="VH663_001453"
     CDS             2666..4642
                     /locus_tag="VH663_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEQ8174641.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYQLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            4639..5091
                     /gene="rplI"
                     /locus_tag="VH663_001454"
     CDS             4639..5091
                     /gene="rplI"
                     /locus_tag="VH663_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEQ8174642.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLAITEA"
     gene            5127..6488
                     /gene="dnaB"
                     /locus_tag="VH663_001455"
     CDS             5127..6488
                     /gene="dnaB"
                     /locus_tag="VH663_001455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEQ8174643.1"
                     /translation="MPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDDFY
                     KYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSANA
                     EYYAKIVAEKAMLRDIIARLTDSVNLAYDEILKPEEVIAGVERALIELNEHSNRSGFR
                     KISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGKTA
                     FVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNV
                     TIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKPEN
                     RQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADI
                     VAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIAQF
                     EER"
     gene            6505..6777
                     /locus_tag="VH663_001456"
     CDS             6505..6777
                     /locus_tag="VH663_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEQ8174644.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
BASE COUNT         2427 a         1118 c         1529 g         2238 t
ORIGIN      
        1 acattactgt aaaggtctgt gatgaaatca atattgacaa tcaataatca tttttttaaa
       61 atagataata acaatcggaa tgatggtcaa agctcatgaa agttgtaggt ttttttgtcc
      121 tgcaatcttc tagggttttg gcccttttta tggtttagct ctagaattga gatggctaat
      181 aggactggga tttagaatca ctagaggtca gataacttta gtttacgagc tagtgcttga
      241 ttgttagaaa tggtaagaat tttttaacta gagatgcagc tgaccaatat tacaccattg
      301 gtggtacaag tttggtaggt gaaaaaacgc atgaaactgt tttacgtgaa accttagagg
      361 aagttggtat tcgaactaag gttaatcaac tggcttttgt ggttgagaat cattttgata
      421 tcgatgatgt tttctggcat aatattgaat ttcattattt ggtaagcccc ttagaagacc
      481 ctaaactaga aatgatagag aatgcaagcg acaggtttgt gaatggattc caatcaatca
      541 actggatcaa atgaatcttg tttctgagtt tttacaaacc gagttggcta aatggccagg
      601 acatattgtt cgcattgaag gctaaactaa agcgtatgat tttaaatgaa tagactgaaa
      661 taaaggattt taaaaaaatg acacatgaat ttacagaaag ctatgacgtc attgtcatag
      721 gtgcaggtca tgctggtgtt gaagccagtt tggccactag tcgtatgggt tgtaaaactt
      781 tattggcaac aatcaacctt gatatgttgg cttttatgcc gtgtaatcct tctattggag
      841 gatctgccaa agggatagtg gttagagaga ttgatgctct tggcggcgaa atgggtaaaa
      901 atattgataa aacctatatc cagatgaaaa tgcttaatac aggtaaggga ccagctgtac
      961 gcgctctccg agctcaagca gataaatccc tgtatgcgcg tgaaatgaaa catacggttg
     1021 aaaaacaggc taatttgact cttcgccaaa cgatgattga tgatattcta gttgaagatg
     1081 gacgagttgt aggtgtttta acagccacag gacaaaaatt tgcggctaag gcagttgtag
     1141 tgacaacagg aacagctctg cgtggcgaaa ttattttagg agaactcaaa tattcttctg
     1201 ggcctaataa tagtttggca tcagtaactt tagcagataa tttaaaaaaa ctcggcttag
     1261 aaattggcag attcaaaaca ggaacgcctc ctcgtgtcaa agcatcttct attaattacg
     1321 atcaaacgga aattcagcca ggtgatgaca aacctaatca tttttcattt atgtcaaaag
     1381 atgctgacta tctcaaagat caaattccct gctggttgac ttatacgaat caaactagtc
     1441 atgatatcat taatcagaac ctttacagag caccgatgtt ttctggcatt gttaaaggtg
     1501 taggccctcg ttactgccca tctattgaag ataagattgt tcgctttgct gataaagaac
     1561 gtcatcaact tttcttagag ccagaggggc gtgatacaga agaagtgtat gttcaagggt
     1621 tgtcaacgag tcttccagaa gatgtccaaa aagatttgat tcattcgatc aaaggacttg
     1681 aaaaggctga aatgatgcgc actggttatg ctattgaata tgatattgtt ttacctcacc
     1741 agctacgtgc cacacttgaa accaagctaa tttcaggact ctttacggct gggcaaacta
     1801 atggaacttc tgggtatgaa gaggctgctg gccaaggact tattgctgga atcaatgctg
     1861 ctttgaaagt tcaaggtaag ccagaactta ttttgaaacg gagcgatgcc tatattggcg
     1921 ttatgattga cgatttagtt accaaaggaa ccttggaacc ttatcgattg ttgacatcgc
     1981 gagctgagta tcgtttgatt ttacgtcatg acaacgctga tatgcgtttg acagagattg
     2041 gtcgcgacat tgggttggtt gatgatgagc gttggaaggc cttcgaaatt aagaaaaacc
     2101 aatttgataa cgaattaaaa cgtctgaaca gtatcaaatt aaaaccagta aaagcaacca
     2161 acgatcgtgt gcaggaactt ggttttaaac cattgacgga tgctatgaca gctaaagaat
     2221 ttatgcgtcg cccagaaatt gattatgcga ctgcagtctc ttttgttggt cctgcggctg
     2281 aagatttaga tgctaaaatc attgaacttt tagaaacaga aatcaagtac gaaggctata
     2341 ttcgcaaagc tttagatcag gttgctaaga tgaaacgcat ggaagaaaaa cgtattcctg
     2401 caaatattga ttgggatgct attgattcta ttgcaactga ggcgcgtcag aaatttaaga
     2461 aaattaatcc tgaaacaatt ggacaagcta gtcgtatttc aggagtcaat cctgcggaca
     2521 tcagtatctt gatgatttat ttagagggaa acggaaaagc tcatcgaaaa tattaaatgg
     2581 aaatgaaatt gtaatctcaa agcaagtgga agctctgtat ttcagggctt ttttatggta
     2641 taatgtgtgc ttaagaggtt ataagatgaa aaaatttcgt tttgaaacca ttcatttgat
     2701 tatgatggga ttgattttat ttggactact ggcactttgt gtcagtatta tgcaatcaaa
     2761 aatattaata ttattagcta tttttctcgt tttgctattt gttgttgctc ttttatggta
     2821 tcaaaaagaa gcttatcaat tatcagactt agctcatatt gagctcttaa atgaacaaac
     2881 agaagataac ctaaaaacgc tacttgataa tatgcctgtt ggagtagttc aatttgatca
     2941 agagaccaac gctgtagaat ggtataatcc ttatgcagaa ttaattttta caactgaaga
     3001 gggttttata caaaacagtt tgattcagca aattattacg gaaaaacgtc gtgaagatat
     3061 ttctcaaaca tttgaagttt ctggtaataa atacacttct tatattgatg tatcatcagg
     3121 aattttttat ttttttgata gctttgtggg aaacaggcaa ttagctgatg ctagtatgtt
     3181 aagaccggtt gttggcatta tttcggttga taattatgat gatatcactg atgatttatc
     3241 agatgcagat acctcaaaaa ttaattcatt tgtagctaac ttcattgatg agtttatgga
     3301 gtcaaaacgg attttttacc gtcgtgttaa tatggatcgc tactatttct ttacagactt
     3361 taaaacatta aatgatttga tggataataa attttctgtc ttagaagagt ttcgtaaaga
     3421 agctcaagat gctcagcgcc ctttaacact cagcatagga atttcttttg gtgaagaaaa
     3481 tcatagccag attggacagg tagctcttga gaatcttaat attgcgcttg ttcgtggtgg
     3541 tgatcagatt gtcattcgtg aaaatgcgga tcatacgaat ccaatttatt tcgggggagg
     3601 gtctgtttct acagttaaac gttcaagaac acgtacccgt gctatgatga cagctatttc
     3661 agatcgaatc aagatggtgg acaatgtttt tattgttgga cataggaaac ttgatatgga
     3721 tgcactaggt tcagctgttg gtatgcaatt tttcgcaggt aacattattg aaaatagttt
     3781 tgccgtttac aatccagatg agatgagtcc agatattgaa agggctattg agcgattgca
     3841 ggctgacgga aagactcgtc taatcagtgt ttctcaagca atgggtttag tgactccaag
     3901 atcactttta gtaatggttg atcattctaa gatatcttta acactttcta aagaatttta
     3961 tgaacagttt caaaatgtta ttgtagttga tcatcacaga agagatgatg attttcctga
     4021 taatgctatc ttaactttta ttgaaagtgg agcaagtagt gctgcggagc tcgtcactga
     4081 gttaattcaa tttcaaaatg ctaaaaaacg tttaaataag atccaagcca gtgttttaat
     4141 ggcaggtatt atgcttgata ctaaaaattt ttctacgcga gtgacaagtc gtacctttga
     4201 tgtcgctagt tacttaagaa gtaaaggaag tgatagtgtt gaaattcaaa atatatccgc
     4261 aacagatttt gaagaatata agcaaataaa tgaaattatt ttacaaggag aacgtcttgg
     4321 tgacagtatc attgtagctg caggagaaaa aaatcatctt tatagcaatg ttattgctag
     4381 taaagctgca gatacgatac tgtcaatggc tcacgttgag gctagctttg tattagttga
     4441 aacagcttct cataagattg ctatttcagc tagaagtcgt agtaaaatca atgttcaacg
     4501 tgtgatggaa aaattaggtg gaggaggcca ctttaatctt gctgcctgtc agttaacgga
     4561 tatcagtctt ccccaagcaa agcacttatt attgaaaact attaatatga caatgaaaga
     4621 aacaggagaa gtagaatcat gaaagttatt tttttagcag atgtaaaagg aaaaggtaaa
     4681 aaaggagaaa tcaaagaggt cccaacaggc tatgctcaaa atttccttat taagaaaaat
     4741 ttagctaaag aagcaactag tcaaagcatc ggtgaattaa aaggaaaaca aaaagctgaa
     4801 gaaaaagctc aagctgaaat attagcagaa gctcaagctg tcaaggctgt tttggatgag
     4861 gacaaaacac gtgttcaatt tcaagaaaaa gttggtccag atggacgtac atttggttcc
     4921 atcacagcta aaaagatttc agaagaattg caaaagcaat ttggtgtaaa agttgataaa
     4981 cgtcatattg ttttagatca tcctatccgc gccattggtc taattgaagt tcctgtgaaa
     5041 ttacataagg aagtaacagc tgagattaaa ttggctatca cagaagcata atataaaaaa
     5101 catcacaaga tttggaggtt atgaggttgc ctgaagtagc tgaattacga gttcaacccc
     5161 aagatttact agcggagcaa tctgttcttg ggtcaatctt tatctcacct gataagctga
     5221 ttgcagtgag agaatttatc agtccagacg atttttataa gtacgctcat aaaattatct
     5281 ttcgggcaat gattactctc agcgatcgta atgatgccat tgatgcaacc actataagaa
     5341 caatcctaga tgatcaagat gatctgcaaa gtattggtgg cttatcctat attgttgaac
     5401 tagttaatag tgtcccaact agtgctaatg cagaatatta tgctaaaatt gtagctgaga
     5461 aagctatgtt gcgtgatatt attgctaggt tgacagattc tgtcaaccta gcttatgatg
     5521 aaattttaaa accagaagag gttatcgctg gagttgagag agctttaatt gaactcaatg
     5581 aacatagtaa tcgtagtggg tttcgcaaaa tttcagatgt attaaaagtt aattacgagg
     5641 ctttagaagc acgttctaag cagacttcaa atgttacagg tttaccaact ggttttagag
     5701 accttgacaa gattacaaca ggtttacacc cagatcaatt agttatttta gctgctcggc
     5761 cagcagtggg gaagactgcc tttgttctta atattgcgca aaatgtgggg actaagcaaa
     5821 aaaagactgt tgctattttt tctttggaaa tgggtgctga aagtttagta gatcgtatgc
     5881 ttgcagcaga aggaatggtt gattcgcaca gtttgagaac agggcaactc acagatcagg
     5941 actggaataa tgtaacaatt gctcagggag ccttggcaga agcaccgatt tatattgacg
     6001 atacgcccgg gattaaaatt actgaaatcc gcgcaagatc acggaaattg tctcaagaag
     6061 tggatggtgg tttaggtctt atcgtaattg actacttaca gttgattaca ggaactaaac
     6121 ccgaaaatcg tcagcaagag gtttcagata tttcaagaca gcttaaaatc ctagctaaag
     6181 aattgaaagt accagttatt gccctaagtc agctttctcg tggcgttgag caaaggcaag
     6241 ataaacgacc agttttatca gatattcgtg aatcaggatc tattgagcag gatgccgata
     6301 ttgtagcctt cttataccgg gacgattatt accgtaaaga aggtgatgat gctgaagaag
     6361 ctgttgaaga taacacaatt gaagttatcc tcgagaaaaa tagagctggg gcgcgtggaa
     6421 cagtcaaact gatgttccaa aaagaatata acaaattctc aagtatagcc cagtttgaag
     6481 aaagataaga aagagagaca aaaaatgagc gatgcattta cagatgttgc aaaaatgaaa
     6541 aaaattaaag aggacataag agcccatgaa ggacagttag tagaattaac ccttgagaat
     6601 ggacggaaac gggaaaaaaa taaaattggt aggcttattg aggtttacag ttcactcttt
     6661 atcattgagt atagtgatag ctctgatact ccaggagcaa ttgataattc ttatgtcgaa
     6721 tcatacacct attcagatat tttgacagaa aaaacattaa ttcgttattt agattgattt
     6781 ttgcctcata gaggcttttt ctttacaata aattgatata attattagcg atagaagatt
     6841 attctgcctg tattcttttt attttgaaaa aattcctata aaagataata tttttaggtt
     6901 agattgaagg aaaaaagagg tcttcaccat ggttaagcaa atgatcttag tactaaggtt
     6961 aaaagattat cgtgaaataa agttttgaca caagtgtaat taatagagga acttgaagta
     7021 tccggtaaaa ctatcttgaa gtgaaaaaac gagtagacct cccctgataa atacaatata
     7081 ctagccattt cagagcaatt aagagtttcc agtgaagtgc ttttgataga gagctaagaa
     7141 aaaaacagtc aaggtttaag aactgcaaat tagcatttaa attatttttt gttgatgaca
     7201 gttgtcttta ttaccataac tataacttct tataatatag tcttcttatt catcagtggt
     7261 ggctcgtctc atatctgacc ttaaaatatt aaactgatca tcagatgata ga
//
LOCUS       DAUGFP010000058         7309 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.058, whole genome shotgun sequence.
ACCESSION   DAUGFP010000058 DAUGFP010000000
VERSION     DAUGFP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.058"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            318..557
                     /locus_tag="VH663_001457"
     CDS             318..557
                     /locus_tag="VH663_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ8174645.1"
                     /translation="MRKQEQDHHKQDVNQKKPEEMTKLERLQEENEYLRTEVAYLKKP
                     RELRLKEASLQKRRQKTIIKNGQRRALIRFSFKNG"
     gene            complement(705..1628)
                     /locus_tag="VH663_001458"
     CDS             complement(705..1628)
                     /locus_tag="VH663_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174646.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1621..2691)
                     /locus_tag="VH663_001459"
     CDS             complement(1621..2691)
                     /locus_tag="VH663_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174647.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2700..3626)
                     /locus_tag="VH663_001460"
     CDS             complement(2700..3626)
                     /locus_tag="VH663_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174648.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3626..5128)
                     /locus_tag="VH663_001461"
     CDS             complement(3626..5128)
                     /locus_tag="VH663_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174649.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYKKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMVGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5193..7163)
                     /locus_tag="VH663_001462"
     CDS             complement(5193..7163)
                     /locus_tag="VH663_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ8174650.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTIGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKAEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDNAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYENAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2283 a         1412 c         1349 g         2265 t
ORIGIN      
        1 ctttttttgt acttttttat ttgcccgagt tgtaaaatga agtgcaacaa aaaagaggaa
       61 aagccttgaa ctacacccca aacgttagac aaaaaaatct aacgttcggg gtatttttta
      121 tgaaattaag tgatgaaggt acgaaataga catcgttaaa aaaaatacct actatccacc
      181 agagataaag caggaaataa tggataacgt tttgatagaa ggcccgccac aataccaagt
      241 atcacttgtt tatggcttgt tacatgcagg aactctccca agttgaatag cacaatataa
      301 gaaaaacagt tatactgttg agaaaacaag agcaagacca tcacaaacag gacgtaaacc
      361 aaaagaaacc tgaagaaatg actaaattag agcggcttca agaagaaaat gaatatctca
      421 gaactgaggt tgcttactta aaaaaaccga gggaactccg tttgaaagaa gcatctctgc
      481 aaaaaagaag acaaaaaacc attataaaga atggacaaag acgagctctg attagatttt
      541 cttttaagaa cggctaaatt gactcgttct acctactatt atcatcgatt attgcaacaa
      601 taaacgtatc aaaataaaac gaaaaggact tagtcctcta caatacaaaa ctaaatcctt
      661 tactaaaaat agagtgttca acttttgggg tcagtgcatc tcttttacaa ttctttttga
      721 tatttttcaa tttctgcttg gtttgcccaa acaaagtgat ttggtttgat ttcaaccatt
      781 gatggcttat ctaatgtata atcatgctgg tctggatgat agacaacaag ttctttttga
      841 cgctctaaaa ttggatctgg gataggcacg gctgataaca aagattgggt gtagggatga
      901 attgggttat taaacagttc ttctgtttct gcaacttcta caataacccc tttatggata
      961 accgcaatac gatctgaaat aaagcgaacg actgaaagat catgggcaat gaagagataa
     1021 gtcaaacctt tttcggcttg catccgtttg agaaggttta aaacctgtgc gcgaacggaa
     1081 acgtccaaag ctgaaatcgg ttcatcagca ataacgaatt cagggttcat tactagggct
     1141 ctagcgatac cgatccgctg acgttgacct cctgaaaatt catgagggta gcgcgtcaaa
     1201 tgctctgata gcaaaccaac ttcggccatc atgttcttaa ttttttcttt acgttcttcc
     1261 tctgttttaa acagattaaa gttataaaga ccttctgaaa tgatgtagtc aacggttgcc
     1321 cgttcattca aactagcagc gggatcttgg aaaatcattt gaattttacg aatgagctca
     1381 ttggcttctg attttgattt tctgccatta attactttcc catcgtataa aatttgacct
     1441 gaactagtat cgttcaaacc aataatagca cgaccaattg ttgttttacc actcccagat
     1501 tctccaacta aagaaaaggt ttctcctttt ttaataaaga aattagcatt tttaactgca
     1561 acaaattttt tctttccttc tccgaaggaa atttctaggt cttttacttc gactaatttc
     1621 tcagacattt ccttcctcct gttgtgacat tttccttaag attttttgat gcaaatcttg
     1681 aatgacttct ggtttttgaa cttttggagc ctctgggtgt aaaagccatg ttttggccca
     1741 atgagtctca gatacgttaa tcgccggagg tgctttttca aagtctaaaa ccatagcata
     1801 ttctgagcga agtgcaaagg catctccgat aattggtgaa taaagtgatg gaggcgttcc
     1861 tggaatagcg taaagttcac cagattcatc tgccaactgc ggtaagctag acagcaaact
     1921 ccatgtatag gggtgtcttg gatcatagaa aatctcttcg actgttccaa attcaacaat
     1981 ttctcctgca tacatgacag ccactttatc tgcaatgctt gccacaacac ctaaatcgtg
     2041 cgtaataaag ataatggtga aatgatattc tcgttgaagc gacttcaata actcaacgat
     2101 ttgagcttga atagtcacat caagggctgt tgttggctca tcacagataa gaatatctgg
     2161 acgacaagct aaagcaatag cgataacaat acgttgacgc attcctcctg aatactcaaa
     2221 tgggtaatct tcaaagcgtt ttttggcatt tgggataccc actttattca tgtaatcaag
     2281 ggccatttct ttagctttgg catgacttac tttttggtgc ttaataatca cttctgtgat
     2341 ttggctaccg atagttttaa tgggactaag actggtcatt gggtcttgga aaatcgttgc
     2401 gatttttgag ccgcgaatct ttgcccactc tttatttgtt tttaaatctg tcaattcttg
     2461 cccacgatag acaattgaac cattagcaat gcgtccatta gactccaaca tccctgtaaa
     2521 ggtctttgtt aaaacagatt tacctgagcc tgattcccct acaaaagcaa ggacttctcc
     2581 ttcaacaagt tccagtgaga cgttacggat agctgttaaa acacgatcac gcacatcaaa
     2641 ttctaccacc acatctttgg cagttaagat tacattattt tcttttgtca ttttaactcc
     2701 tatctatgtg aacgtgggtc actggcatca gccaagtttt gtccgacaat gtatagtggt
     2761 aacgatacta aaatcaatgt tactaaggga atccaaaaga ggtaggcatt tgttgttaag
     2821 ttgcttgaat aattagcaat caaacgtcct aaacttggag tggtggttgg taaaccaatc
     2881 ccaaagaagg ataagaaggc ctcagaagat acataaactg gtagcatttg tgacaacata
     2941 gtcatgataa ctgaaaccaa ttgaggcagg aggttcttaa cagcaatctt gtacattggt
     3001 gttcccaaag tttgactagc aaggttgtat tctaaatcac ggtaacgcaa gatttgaaca
     3061 cggatggagt aggcgacacc aatccatcca gtgatacaga aagctagaat caaattccaa
     3121 aatcctgcac ctaatgaata ggtcaaaaca atgataataa gcatagaagg gatatttgag
     3181 ataatgttat aaatttcaat cataacttta tcaaatgctt tagaaactcc ccatatggct
     3241 cctaacacta ccccaatggt gatattaatt agtgtcgcta taactgagat taaaatagaa
     3301 ttacgtgccc cataccaaac accatcaaac agagattgcc catttttgtc ggttccaaac
     3361 cagtactctg cattcggcca aatataacgc tttgaaaagt cattgatatt actaacgtca
     3421 ttaaagtcgt agttggcaaa cattggataa ataaagctca tcatcaagac tgtcactaaa
     3481 atcacgagca taaagactgt agatttttta gaaaaaaact gacgaaacac tgatttccag
     3541 taagaataag caggggtatc aatcacttcg gaggcttcac tatcgcgctc aacaaatcga
     3601 aatttagatt tatcaatcga ttccattact tacctccttt ttttgttgac aatttaatac
     3661 gtggatcgac aagggtcatg acaatatctc ctagtaaaag tgaaacaatt gacaagacag
     3721 tgaagataaa ggttaatcca acaatcatag aattgttagc agatttaata gaatcaatta
     3781 acattttacc catacccggg aaggcaaaga cagtctctgt taaggttgca ccaccaatag
     3841 caaggataat acttgcagga acccctgaca caattgggac catggcattt ttgaagagat
     3901 gccctctgta aatttcagat tctgataatc cttttgaacg cgcaaaacgt acccaatcgc
     3961 tggcctgaag gtcaacaaga tagcgacgga accagataac cgtagttgga atatttaaaa
     4021 ttcctaaaat aagggctggt aaaatataag atttaggatc agatgccccc agcattggga
     4081 aactatctgg taaaccaacc atgccaccta ggaagcgaac aacatagata acagcaatac
     4141 ttggtaaagc aagcatgaag gtcatggtcg ctgttgagaa acggtcaaaa taggtatttt
     4201 taaaccgagc cataaataaa ccaagaggca aaccaacgat ataggaaaac ataacaccaa
     4261 aaaaaccaat tacaaaagaa ttgtgaatca ttgatgggtc gacataatta ttgatggttt
     4321 tagtgtagga atctccctta ccatagttta ccttggtgat atcatccaat gattttggat
     4381 ttttgtagct acggctatag atatccactg acgatttctt agtgacacct gatgggaagg
     4441 tcacatcttg aagagctgtt cgtccttgcc cttgtgaaat aacctgaaga acaggaatat
     4501 tgctgtatgt tggataagat gttcctaagt ttagggtaac aaagttttgg tgaagataag
     4561 gaaattttcc gttagtatag aggagatatt tatgttttgt ccccgaacca acaagtgacc
     4621 agccaactga tttatctttt tctagacgaa tatacctagc taattttgga ttatctttat
     4681 cctgaatctt ccaagggtga tcaataacaa ctagatttga gaaaaagttc caaacacgtt
     4741 cgtaaatagg aatattgcga attgcataaa actgtttgct agtggggaag cgttttagct
     4801 gccagccatt gccaagagaa tcaatatact tcttatagat agctttgtta gcactagttg
     4861 cttctgtcgt tacactacta tctactttag aagcattatc ttttaattct ttgcttgaaa
     4921 aataattgac ataacccata cgttgaaaag ttagattttc atacgctact tttttatctg
     4981 gtgttgttgt cattttatta taattagggt cttgtttaaa gataaggctg gtaggtacta
     5041 gagtatatac gatggtgtat gttaaagccg ttaccaaaac taccgacact aacgatcgca
     5101 tgatacggtt taaaatgtat tttttcatag ttatcctcaa tagttcaaaa gaactccctc
     5161 acataaacga gaaagttctt ttgaacatcg ttttattttt caacgtgatc agctaacttc
     5221 tcagcatatt ttgcatttga cttagccttt gctttcatcc atttttcttt tgcattttcg
     5281 tattgttttg ctgtgacagg tttgtcttga agtttcattc ctttatatgc tagaggacct
     5341 ttagaccctg cccaagagaa gcccccgcta aatggaacgg ctttagtaac tcgtggagtg
     5401 ccacccaatg ccacaactgg aatatctacg gcattatctg taaggtaggc ttgtgctttt
     5461 gcgtaagctt tatagcgcgc atcgttgtcg tctgtaattg ctgctgcttc atcaagaaga
     5521 gtttggtagg tatcaagacc tgcagctgcc acaacatcct tattttgacc tgctttgatt
     5581 ccaagttttt ggataacaga tccaccacct actggactca tgatgtcaag gtaggtccgt
     5641 ggatcttgat agtctggtcc ccaccatgat gaaatgatat cgtagtcttg ttgttctggg
     5701 gtctcagcat agaagccttg ggcttcgtga gttgatgttt ctgtttcaag aacattgaca
     5761 atgacatttt ctttaccaag agatgcttca acagattgtt tgaaagactg ggcttcctga
     5821 acagttgctg cgtttgcttg gtcaacaggg taatctaatt gaactgggaa ggttacacct
     5881 tcagctgtca aagcttcttt ggcttttgca aactcagctt ttgctttttc aggattatag
     5941 aaaccatctt gagcatcagc taagttaacg tctttccatt catcaccaag ttttgccatt
     6001 tccttttcaa cttctgaacc aaaatcactt tctccaatgg tcacaaatgt tggtggcaca
     6061 agcatgttac gtaaggcttt tgttttggca tcttgacctg cagtttgggc ttggaatgac
     6121 gctcggtcaa aagcaaactg aatagcttga cgaaaatcct tgttgttaag agctttctta
     6181 ccggcatctt gttgtgcagg gtctttctta gtgtttttga aagaagtacg gttcaaattc
     6241 catgttaaat gacggatatc tccagtcaac attccgtaag taatgttatc agcatagttt
     6301 ttcttagctg atttgtaggt agggtcattt gggtaaagtc gtgcaacgct gaactcaccc
     6361 ttgtcaaagt tcttgtagaa cgaacctggg tctgaaccat ctgagtaagt caatttaaca
     6421 gattctatcc caacattctt agcatcccag tagttttcat ttttatggaa ttccatagat
     6481 gattttgagg tgaaggcgct caagaagtaa gcaccattaa caaggattga tgatggatcg
     6541 gttgtaccaa aatctttacc ttttgacttc aaaaatttcg cattaactgg gaaaagcaca
     6601 ctataagttg tttttgaatt ccagtagctt tcaggcttgt tcaaagtata ctgaacagtt
     6661 ttatcgtcaa gggctttgac accaacttct ttaaaatcta cttcaccatt ttggtaagcc
     6721 tttaagtttt ttattgaatc ttcaacaacg taaagagcat ctgatttatc gtcaaccgcg
     6781 tgcttcaaac cagtcacaaa atcttctgct gttactgggg catattcttc accatcagcc
     6841 gtataccaag agacaccatc gcgaagagta taagtataag tcagaccgtc ttttgaaacc
     6901 ttccaatctt tagcaagtga tggtactaaa ttaccatact catcgttttc caaaagacca
     6961 tcaaccattt gtgttatcac atcagtcgtt ccaccgccat tagtcaaaat ataatccaat
     7021 gattttggat cgttaacaaa aacgtacttg taggtttttg tagcttctga gccacctgaa
     7081 gcatttttat taccacaagc tgccaaagcg gatactgaca agatcgcaac acttacagct
     7141 gccaaccatt tacttttctt cataaaagtc acccctaaac tttatttttc attttttggc
     7201 aattgctaaa tgaaattggt taattgaaga cattatatca taaaactttc ttaaagaaaa
     7261 taatgaaatg aaaaattatc tgaatatttc gtagaatttt ctttatttt
//
LOCUS       DAUGFP010000059         6990 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.059, whole genome shotgun sequence.
ACCESSION   DAUGFP010000059 DAUGFP010000000
VERSION     DAUGFP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.059"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(364..1770)
                     /gene="lacG"
                     /locus_tag="VH663_001463"
     CDS             complement(364..1770)
                     /gene="lacG"
                     /locus_tag="VH663_001463"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEQ8174651.1"
                     /translation="MTKTLSKDFIFGGATAAYQVEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNPDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFAALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGTNVKGYFMWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(1858..3555)
                     /locus_tag="VH663_001464"
     CDS             complement(1858..3555)
                     /locus_tag="VH663_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ8174652.1"
                     /translation="MNGLIAQIEKGKPFFEKMSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIESLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFMSTMLAAIVGFLLMAANPAKEGGFLTGFMGTKGLLTAFIAAFITVNVYKTCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFIWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLVVVDVIIYYPFLKIYDEQILAEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQVALDFVKAQFEN"
     gene            complement(3555..3872)
                     /locus_tag="VH663_001465"
     CDS             complement(3555..3872)
                     /locus_tag="VH663_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ8174653.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(3896..4879)
                     /gene="lacD"
                     /locus_tag="VH663_001466"
     CDS             complement(3896..4879)
                     /gene="lacD"
                     /locus_tag="VH663_001466"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ8174654.1"
                     /translation="MTITLTENKRKSMEKLSVDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEAGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDVNEQKKAYIERIGSECSAEDIP
                     FYLEILTYDEKIADNASPEFAKVKAHKVNEAMKVFSKERFGVDVLKVEVPVNMKFVEG
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLEFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            complement(4883..5812)
                     /locus_tag="VH663_001467"
     CDS             complement(4883..5812)
                     /locus_tag="VH663_001467"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEQ8174655.1"
                     /translation="MILTVTLNPAIDVSYPLDELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNEFGETVKATGCIGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPMLSVDEIDGFTHHFKYLLNDVDVVTLSGSLPAGMPDDYYQKLIGIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTRDFDALKEVLQDKLFDGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKIKEV"
     gene            complement(5860..6375)
                     /gene="lacB"
                     /locus_tag="VH663_001468"
     CDS             complement(5860..6375)
                     /gene="lacB"
                     /locus_tag="VH663_001468"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ8174656.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHEIIDCGTYDNVRTHYPI
                     FGKKVGEAVASGKAELGVVICGTGVGITNAVNKVPGIRAALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(6410..6838)
                     /gene="lacA"
                     /locus_tag="VH663_001469"
     CDS             complement(6410..6838)
                     /gene="lacA"
                     /locus_tag="VH663_001469"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ8174657.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKHEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
BASE COUNT         2160 a         1434 c         1138 g         2258 t
ORIGIN      
        1 aagatttttg agatgtcaca taagaagtag ccctctgata taaaaaacaa aactcgttct
       61 ttacagctat tttacaatct aatcgcttct cgctataaca tcaatctcta atcagaaaat
      121 ggttacttta cttatttaat caggtcagga gtttgaatga cagaagtcaa atatctgaaa
      181 aaggatgtta aataaaactt tcaaaaccca ttttcaccaa ctaaaaacac ttataacccg
      241 atcttacaac taatggatta ccatcaacag caaataatac tatgcttgga aaatgtcaac
      301 atcatctact ttttgtgact acgagcatga cgtgtaaaaa tgtattaaaa aagtatctca
      361 aaatcattca atcacttgag tttctgctac ttttttatac cagtaggcac tcttcttagg
      421 ataacgttct tgagtttcaa aatcaacata gaagagaccg taacgttttt catagccatt
      481 tgaccatgaa aagacatcca tcagtgacca cataaagtat cctttaacat ttgtaccatc
      541 agaaatagca tcagaaataa cttctaagtg ttttttcaca taatcgatac gtccatcatc
      601 ataaacagta ttatctacaa actcatcttt gtaaccaaga ccattttcag tgatataaat
      661 tttcttgtag ttaggataat cggctttgac acgcatgatt tgatcatata agccttgtgg
      721 gaagataatc caatcccagt ccgtttttgg aacatcaacg ggagcttttc ttcgacccac
      781 acccttgatt tggtatttag agctgccttt ttccccttta ccattatgaa tgatttcagt
      841 ctcaccatca aaagcttgca tccaatcact catataatag ttaataccaa ggaagtcatt
      901 caaatctttt gcagcgtcta gtgcagcaaa atcttcctca cgaagatcaa gttcaccacc
      961 atttacctca aggatatggt taacaccttc cattgtttta tctgaatact tacctagata
     1021 agttgcatca agaataaatt tattatggat gatatcttcc agttcagctg ctctaacatc
     1081 atcaggattg ttagcgtcaa atggatactt agttggaaga gcatggacaa cgccgatttc
     1141 acctgaataa ccactatctt taaagagttt gactgcacga gcatgagaga ccatcatgtt
     1201 atggtgtgat tggaaaactt tagcaagatc atattggata cctggaggga atttaccaac
     1261 caagtattgg ccatcaccaa taggcccaat ttcgttaaat gttgtccaat agttaacttc
     1321 tgagaattct ttaaaacaaa attctgcata atttacaaaa tgttcaatgt tctcacgatt
     1381 gaggaaatca ccatccgagt ggagagcttc tggtgtatca aaatggtgaa gtgtaacaaa
     1441 aggctcaaca tgacgcttat gacactctgc aaagagattg tggtagtatt caactccttt
     1501 agggttaact tctccttttc ctgttggaaa aatacgagac caggcaatag agatacggat
     1561 gccgttgaca ccaaattctt cactaagttt caaatcgact gggtaacgat tataaaaatc
     1621 acttgctggc tcagctgtgt accaatagtt gtcttctaag tatttatccc aagctactgg
     1681 tcctttgcca tctgtatggg tagcaccttc aacctggtaa gcagctgtag caccaccaaa
     1741 aataaaatct ttagataatg tttttgtcat ctcagtttct ccttgaatat ctttcaaaaa
     1801 taaagttccc aaaacaattt ttgtgtttga gaactttacg acatttaaac tgttttctta
     1861 attttcaaac tgtgctttta caaaatcaag agctacttga ccatcacggg tcaatttgat
     1921 gtattgagcc ccttctgttt tagctaattt aatatcaagt ttatcagttt ccactttcat
     1981 atcttcatag ttagaagcta cttgaggtgc aaggataaca agttgatatt gtggtaaaat
     2041 ctcacggtga gcaccataac ttcctgctgc tgcagtgaca ggtgcaccat attctttagc
     2101 tgctttattc aaagcgtttg caagcaaacc acttgtacca ccaccggcac aaagaacgag
     2161 aacattagtt tgatcagtga tttctttggt ttctttagct gctgattttt caagaatagc
     2221 atcagctttt ttagtatcaa agtttgctgc tactttttct ttgagggcat ctgatgaatt
     2281 cgtacctgct gcttcttcag caagaatttg ctcatcataa attttcaaga atgggtagta
     2341 gataatcaca tcaacaacaa ctaacaaagc tgccaaaatg aatgctagca cttggaagtt
     2401 tgtaccaagg ataagaccca atggagctgg tgttgtccaa ggtaagtttg cagtgaaact
     2461 gttcattcca agtgtgtcaa tgaagaattt gaaaatccat acattgacaa ttggtgccaa
     2521 gataaatgga ataaagaaga ttgggttcaa aacaagtggg gcaccaaaca aaattggttc
     2581 gttaacacca aagaatgttg gaacaaccga tgcacgtcca atcgctttgt tacgttttga
     2641 ttttgttaac caaatgaaca tgaatggaac gacgagagtt gcaccagtac cacccatggt
     2701 aacgatgaac atttgagtcc ctgaagtcaa aatcttgtca gcatgttcac cagcttgaag
     2761 taattgaagg ttggtttcag catttgcgta agtaatcgct gcaatggctg gttcaacaat
     2821 agatggtcca tgaataccaa caaaccagaa gaaagcaaat gcaccgaaga taatagtgat
     2881 tccaacatag ccatctgctg cttggaacaa tggtgcaaga agtttaccaa ttgattctgc
     2941 aacgttaacg cctaaagtag tatgaacaat caattcaatc acataaagca gtacaactga
     3001 taacgtaaat ggaatcaaat ccttaaatac ttgagagata tttggtggaa cctcttctgg
     3061 catacggatt gtaacattgt ttttcacaca ggttttatag acatttacgg taataaaagc
     3121 tgcgatgaag gctgtcaaca aaccttttgt tcccataaat ccagttaaga aaccaccttc
     3181 ttttgctgga ttggctgcca taagtaaaaa acctacaata gcagcgagca tggtagacat
     3241 gaagttgatt tggtttgtgc ttggtaatga tcgattcatt gagtcagtta aagctttggc
     3301 tgtcgtacca gccacaaaca aacctaaaat acccattgag tagttgtatg gcgtcatcaa
     3361 gagactctca atatccttac tccaatggaa gccccatgca tttggaacat aggcaatcaa
     3421 gataaagata cttgaaaaca agataactgg cataccagcg ataaaaccat cacgaatagc
     3481 gcgaagatag atgtttcgag acattttctc gaagaaaggt ttccctttct caatctgagc
     3541 aattaatcca ttcattattt tgctcctctc ttataaagtt caatcatgtg tttcattaaa
     3601 tcttgtaata aaatggtcgt cattaaatga tcttgaccat gcattaaagt gacactataa
     3661 gctaagtcat ctccagctgc ttctttttgt agtagacttg tctgagcatt gtgagcatca
     3721 ttcaagcagt cactacctgc agcgattaat tcttctgctt tagcataatc tccttcttgc
     3781 gctgctttaa gcgcttccaa aaattttgaa cgagcatcac cagcataggc tacgatttca
     3841 aaacctaaaa gagtagtttc ttctctattc attgtagttt cctccgaaaa taatgttaca
     3901 ttttttcagt ccaaggactt gcagttttat ctagtacttt gtttaattcg tcaatatttt
     3961 tgaaaccttc tgtacgcaac cattcacgag ctgcttgtgg tccttcttcg atatagactt
     4021 ttacagatcc tgcccaggta gcacgtccac ataagacacc gttgaattta gcacctgatt
     4081 ctgctgcaaa ttccaaagta tcttggaaca acttggctga aacaccagca ctcaaataaa
     4141 tgtatggtaa atcagtagaa gcttcttgat cacgaaaagc ttgagctgct tcttcttttg
     4201 taaagagaac ctcaccatct gcaaagcctt ctacaaattt catatttact ggaacttcaa
     4261 ctttcaacac gtcaacacca aagcgctctt ttgagaaaac tttcattgct tcgttaactt
     4321 tatgagcttt aacttttgca aactcagggc ttgcattgtc agcaattttc tcatcataag
     4381 ttagaatttc taagtagaat ggaatatctt ctgcactgca ttcagaacca atacgttcaa
     4441 tgtaagcttt tttctgctcg ttaacatctt gatctccatc aatatcatag taaagaagga
     4501 atttaactgc ttctgctcca gcttctttaa tacgttttgc agaccaaaca tctaagcaat
     4561 caggtaagcg actagttgtt gttgcatcgt atcctgtttt ctcatatgcc aacaacaagc
     4621 ctgcttcttc tgaacgaaca cgacttgctg gcaagccata ttcaggatca agtagaattg
     4681 aggatgcaaa tggtgttagt tcttcagaaa ctaaactttt aagctcttca atttgttcta
     4741 ctgttggctc ttttgtttga tgttgagcca tcatacgttt gagagctcca cgttggtcaa
     4801 aagctaaggc agaaataaca ccatctacac ttagtttttc catgctttta cgtttgtttt
     4861 cagttaaagt aattgtcatt ttttatacct ctttgatttt aatagattga tataacttat
     4921 cgtaatttgc catgttaaca tgcccagttg ttttttcttg cgcatttaac atccctaaga
     4981 catttgcttt tgttaataaa gctcgatcat cttcatcatt cgctaaacct gaagcaatac
     5041 ctgccactgt agaatcccct gaacctacag cgcttacaat tttaatttta ggaatgtcaa
     5101 cgttataaaa cgtatccttg tgctttgcaa atacaccatc agcaccgaga gaaacgatta
     5161 tccattcaat tccatcaaat aacttatctt gtaagacttc ttttaaggca tcaaaatctc
     5221 ttgtcatttc tttacctaga agttgagaaa gttcttcaag attaggcttg ataactgttg
     5281 gtttactatc accttttagt actgcttcta aagcatttcc agaacaatct agaacagtct
     5341 tcttaccatt aagattggca ataccgatta acttctgata gtaatcatct ggcataccag
     5401 caggcagact gcctgagagc gtcactacgt caacatcatt cagtaaatac ttaaaatgat
     5461 gagtaaatcc atcaatttca tctacactta acatcggtcc tttttctaaa atctccgttt
     5521 gattaccttc gtgtaaaatg gctatacaag ttctagtatc acctgaaatt ttatagaagc
     5581 gacttaaaat cgaatcagga agatgattaa tgataaaatc accgctttct ccacctatac
     5641 aaccagtagc tttgactgtt tcaccgaatt cattaagaac tcgggaaaca ttcaagcctt
     5701 taccaccggg tgtctttgta acgtctacaa ctcgattgac agtatcgcat tttagctcat
     5761 ccaaagggta ggagacgtca atagctggat ttaaagtaac tgttaaaatc acaatagctc
     5821 ctttttctat atatatccct atagtaattt attcaaatat tagtcgtgat attcaccacg
     5881 gtcccatttt tctaggaatt catcaaagaa gtgtgggttg ttttgatctt ggctagcatg
     5941 ttgcaagcta tccatttttt caatccattt tttattttct tcagttggtt tgtattccgc
     6001 ggctaagaaa gcatcgataa tatcattcat caataaacca ccgatgattt ttccaccaaa
     6061 accaataacg tttgcgttta gctcttcttt tgagtagata gcagatgtca tatctctaac
     6121 gagtgctgca cgaataccgg gaactttgtt tacagcattt gtgataccaa caccagtacc
     6181 acagataaca acacctaatt ctgctttacc acttgctact gcttcaccaa cttttttacc
     6241 aaagattgga tagtgtgtgc ggacattatc gtatgtcccg caatcaataa tttcatgacc
     6301 ttgtgatttt aggtaatcca caacagcgat tttttcataa gtaacaatat ggtcacaacc
     6361 tacagcaatt ttcatttttg ttctccttgt tttaacctat ttcaatcaat tagcacattt
     6421 tattaagcat gtcgacacga acttgatgac gtccaccatc atatttacct tcgacaaagc
     6481 ctttgataat gttctttgca attcctggag cactaatttc agaaccaagc gtgatgattc
     6541 gagcattgtt gtgtcctctt gtcatgtatg ctgagcgctc atctgatact tcagctgcca
     6601 ccattccttt gattttagta gctaccatga aagaaccaac accatatgca tcaatgacaa
     6661 tacctaagtt atcttcggat gcattaactt cttttgcaac agctaaagtt gtatctacaa
     6721 agtcacgtac ttcgttttca ctaacatcaa cgacttcgtg tttaccttct ttaaggtaat
     6781 ctttgataac ttctttaagt tcttgacctg ctttgtcagc accgataata attgccattt
     6841 tatttctcct atgatttaga ataagtaaat gtggaaaacc tacgtagata gttaattaac
     6901 gatttgcaac atcttttgaa ataacgcttt cattatttct atgacctaat catacaccca
     6961 ccaaacataa aagtcaacat tttttgttta
//
LOCUS       DAUGFP010000060         6638 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.060, whole genome shotgun sequence.
ACCESSION   DAUGFP010000060 DAUGFP010000000
VERSION     DAUGFP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.060"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            109..2307
                     /gene="nrdD"
                     /locus_tag="VH663_001470"
     CDS             109..2307
                     /gene="nrdD"
                     /locus_tag="VH663_001470"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEQ8174658.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIVKAMKQACEDWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            2489..2635
                     /locus_tag="VH663_001471"
     CDS             2489..2635
                     /locus_tag="VH663_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174659.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRLQELKARFLEKSK
                     KQDN"
     gene            2664..3599
                     /locus_tag="VH663_001472"
     CDS             2664..3599
                     /locus_tag="VH663_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ8174660.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGQAVLYSDVKELPKASLDGVFIHAATSAHAELASVFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKELSSLSTKRKVAVEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLTEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIAASQLSYGELKVELPKD"
     gene            3608..4102
                     /locus_tag="VH663_001473"
     CDS             3608..4102
                     /locus_tag="VH663_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ8174661.1"
                     /translation="MEIRRPTLEDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLASQGVPAIQYVAFDEKNQALGFLNLRLRLNASLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSCAISKNITTILVTCDETNVASRAVIVANGGVLEDSRGGTERY
                     WIEE"
     gene            4117..4731
                     /gene="nrdG"
                     /locus_tag="VH663_001474"
     CDS             4117..4731
                     /gene="nrdG"
                     /locus_tag="VH663_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEQ8174662.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRRELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(4905..5636)
                     /gene="yaaA"
                     /locus_tag="VH663_001475"
     CDS             complement(4905..5636)
                     /gene="yaaA"
                     /locus_tag="VH663_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEQ8174663.1"
                     /translation="MLTFLIPTAKEMMIPKESHPHLLPQDSQAILKIMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQSLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFHYHPDLSTDHEFVYIKKEA"
     gene            complement(5678..6535)
                     /locus_tag="VH663_001476"
     CDS             complement(5678..6535)
                     /locus_tag="VH663_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ8174664.1"
                     /translation="MTYPYNSTISLGTVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTAFLVPDRHSLGLVLNHFLTRGISLEGAAD
                     HGYSEAIYLSDPEDNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKR
                     FLLAQDTRIGHVHLSVKNALASSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHW
                     SAPYLKKHQEGAPGLAFLTIHIETPLLFSATLKNARLHGLAILQEDSSSFTTEDEEGI
                     CVNVILGQD"
BASE COUNT         2011 a         1106 c         1523 g         1998 t
ORIGIN      
        1 cagaaaaagt aatctaatat caatatattg tgttcgagca aaaaacttga cacaatatat
       61 tgattttttt agttgtcgtg atataatagt tccgtaagga gatttgatat ggtaagttta
      121 gaagaagaca aggtgactgt tcaacctgat attaaagtga ttaaacgaga tggtcgcctt
      181 gttaattttg atagtacaaa aatctatagt gctttattaa aagcaagcat gaaagtaact
      241 cggatgtcgc cacttgttga ggctaaatta gaggctattt ctgatcgcat tatagcagaa
      301 attattgagc gttttccaac taatatcaaa atttatgaaa tccaaaatat tgtagagcat
      361 aagcttcttg cagctaatga atatgctatt gcaaaagaat acattaatta tcgtactcag
      421 cgtgactttg cacgttcaca agcaacagat atcaattttt ctattgataa attaattaat
      481 aaagatcaaa cagttgttaa tgaaaatgct aacaaagata gcgatgtttt taatactcaa
      541 cgagatttaa ctgctggaat cgtagggaaa tcgattggtt taaaaatgtt accttcgcat
      601 gttgctaatg ctcatcaaaa aggagatatc cattaccatg atttggatta cagtccttat
      661 acaccgatga cgaactgctg tttaattgac tttaagggca tgttagccaa tggctttaaa
      721 attggtaatg ctgaagtgga aagtcccaag tctattcaaa ctgcaacagc tcagatctca
      781 cagattattg cgaatgtagc atcaagtcag tacggcggat gcacagctga tcgcattgac
      841 gagtttttag ccccatatgc ggagcttaac tttaaaaaac atatggctga tgctaagaaa
      901 tggatcgttg agactaagag agaaagctat gcttttgaaa agactcaaaa agatatttat
      961 gatgcgatgc agtctttgga gtatgaaatt aatacgctct ttacgtctaa tggtcaaaca
     1021 ccatttactt ctttaggatt tggtttgggg acgtcttggt ttgaacgtga gattcaaaaa
     1081 gctattttga ccattcggat taatggtctt ggtagtgaac atcgcacggc tattttccct
     1141 aaattaattt tcacggttaa acgtggcttg aatttagaac cagattcacc aaactatgat
     1201 attaagactt tggctttaga atgtgcgact aagcggatgt acccggatat gttatcttat
     1261 gataaaatta ttgatttgac aggatctttc aaatctccaa tgggatgccg ctctttcctt
     1321 caaggctgga aagatgaaaa tgggcaagat gtgacctcag gccgtatgaa tcttggggtt
     1381 gtcaccctca atttacctcg cattgccatg gaatcaaatg gcgatatgga taagttttgg
     1441 gagctgttta atgagaggat gctaattagt aaggatgctt taatttatcg tgtcgaacgt
     1501 gtcacagaag caaaaccagc aaatgcccct attctttatc aatatggtgc ttttggaaaa
     1561 cgtttggaga agacagggaa tgtaaatgat ctctttaaga atcgtcgtgc aacagtctct
     1621 cttggctata ttggtcttta tgaagtggca tctgtttttt atggtggtca atgggaaggt
     1681 aatccagatg ctaaagcttt taccttgtca attgtcaagg caatgaaaca ggcctgtgag
     1741 gattggtcag atgaatatgg ttatcatttc tctgtttatt cgactccatc agaaagtttg
     1801 acagatcgct tttgtcgttt agatacggaa aaatttggca ttgtgacaga tattacggat
     1861 aaagaatact atacaaattc ttttcactat gatgtgcgta agagtccgac accttttgaa
     1921 aaattagatt ttgaaaaaga ttatccagaa gcaggtgctt caggtggttt tatccactac
     1981 tgtgagtatc ctgttttgca acaaaatcca aaggccttgg aagcggtttg ggactatgct
     2041 tatgatcgtg tggggtattt gggaaccaat acgcctattg ataaatgcta taattgccaa
     2101 tttgaaggcg attttacccc aacagaacgt ggttttactt gcccaaactg tggcaataat
     2161 gaccctaaaa cagttgatgt ggtcaaacgt acatgtggtt acttggggaa tcctcaggcc
     2221 cgcccaatgg ttaacggtcg ccataaggaa atctctgcgc gtgtaaaaca tatgaatggt
     2281 tctactataa aatacccagg cctgtaaact caagctgggg aagagccctt ttgatggaaa
     2341 tatgatatgc ttaaaaggtt gcttcatgca agcaatcttt tattgtttaa tggtttctta
     2401 gcaattgtga gataagaata actaatccaa aacgactaat tagctgttag caatcggact
     2461 ataagaagaa agagataagg aagaaataat gggaaaatat caattagact ataaaggaat
     2521 gcagcaggta gaacgctttc atgagaagca ttctaaaaaa aagacggata aaaaatcccg
     2581 ccttcaagaa cttaaggctc ggtttttaga gaagtcaaaa aaacaggata actaaagtat
     2641 ccagtaaaga aagaaggctg agtatgctta atattgggat tgtgggacta ggtgctattt
     2701 cacaaaaagc ctatttacca tatatgagac agctgagtga cattacttgg catctatcaa
     2761 cgcggaatgc agcagtacgt cagcaggtcg gtcagttatt tggacaggct gttctttaca
     2821 gtgatgtcaa ggagttgccg aaggcaagtt tagatggcgt ctttattcat gcagccacat
     2881 cggcccacgc agagttggct agtgtgtttt taaatcaggg aatcccagtc tttatggata
     2941 aacctatagc tgacaattac ctgatgacaa agaacctcta tgacttagcc aaagaaaatc
     3001 agacttttct gatggcagga tttaacaggc gttttacacc tcgtgtcaag gaattgtcaa
     3061 gtttgtcaac taagcgtaaa gtggctgttg aaaaaaatga cttgaaccgg ccaggagaca
     3121 tgacttttaa gctctttgat ttctttatcc atcctttaga tacagcctta ttcttgacag
     3181 aagggacctt gctaaaaggg cattttcaat accatctgga agcaggctta ctgagtcaag
     3241 ttatggtaac cttgatgacc gaaagcatga cgactacagc ttctatgaat ttacaatcag
     3301 ggagtcgccg tgaggtaatg gaagttcaac gggctgaaga aacttatcat ttagagaact
     3361 tagacgagtt atctatttat aaaggtactg aaaaaagggt gcttggcttt gcttcttggg
     3421 acaccaccct gcacaaaaga ggttttgaaa caatgatcga tgccttttta gaagctatta
     3481 gcacaggcgt caatcctgtc agccctgagt ctagtctttt aagccactgg atttgtcaac
     3541 agattgctgc ctctcagctt tcttatggag agttaaaggt agagttgcct aaggattagg
     3601 aattaatatg gaaataagac gaccgacttt ggaagataaa gatgctgtat tgtcaatgat
     3661 taacgaattt ttagagcaga aaagtgctac agatgggtta tggcatttta acgtcaatga
     3721 ctttaactat gaaacatggt tggaagattc cttgcgacag gaaatgggtt tggccagtca
     3781 aggagtgcct gctattcaat acgtcgcatt tgatgagaaa aatcaagctt tgggattttt
     3841 aaaccttagg ttacgtttaa atgcttcatt acttgaaaaa ggtgggcata ttggttactc
     3901 tgtccgtccc agccagcgtg ggaaaggtta tgccaaggag atgcttaaac aagctgtcag
     3961 ctgtgcgatt tccaagaata tcacgacaat tttggtaact tgtgatgaga ctaatgttgc
     4021 aagccgagca gttattgtgg ctaacggcgg tgtcttggaa gatagtcgag gtggaacgga
     4081 acgttattgg attgaagaat aaagaggcga taagtgatgg cagaaaagtg ttggaataat
     4141 cctaagccaa aggaatggca ggcagaggag ttaagtcaag gccggattat tgactataag
     4201 gcttttaact ttgttgatgg tgaaggggtt cgtaattccc tttatgtgtc tggttgttta
     4261 ttccattgca aggggtgtta taatgctgcg acttggtctt ttaaggcggg aatgccttat
     4321 acgcaagaac ttgaagagca gataatgacg gatttggcac agccttatgt tcagggactg
     4381 acacttttag gtggagaacc ctttttgaat acgggtatct taattcctct gattaagcgt
     4441 attcggcgtg aattgccaga aaaagatatt tggtcttgga cgggttatac ttgggaagaa
     4501 atgatgcttg aaacaccaga caaactggaa atgttgtcct taattgatat tctggtagat
     4561 ggtcgttttg atatcacaaa aaagaacctc atgttgcaat ttagaggctc ttctaaccag
     4621 cgaattatcg atgttcaaaa gtctttggct gctaaagaag tgattatctg ggataagtta
     4681 aacgatgggg accaaacctt tgaacaaatt agtcgggagg atttgcttta agaagctgac
     4741 ttatccacat gatttttttg aaaaggtcat gtgaaaacag gtcctttaag ggtggattgg
     4801 ggagaaagta attgttattc cctggttttc cccacattat cctcatagtt atccacaggt
     4861 tgtgtataag ttgtagaatt gtgaataaag actaaaaaag aggcctaggc ctcttttttg
     4921 atatagacaa attcatgatc tgttgatagg tctggatggt agtgaaaacc agcaaagaca
     4981 aggcttttaa ggctgtcaac agtttggcaa ttattttcca tacaggcagt gagaaatgca
     5041 cctcgtgcct tttttgaaat ggtggaatgg gttttgaatt ggccctcttt ttctgccata
     5101 aacttgggac ttatccacaa ttgtttacag tctttggaaa agacatcgtc aaattcactg
     5161 gataaaaggg aaatcacttg gggatgctct ttggcaaact gattataaca gggccgccag
     5221 taagacttga ggctttgtcc ttcaattttt attctggtgt gaaaatcatg gcggtgctct
     5281 gcgataggat gattagcggg aatgataccg taaaaggatg aggtgatgta aacttgctgg
     5341 gtgaggtaag cctgttcttg ggtagtgagc ttatcgcgct tgatatggcg gtacataagg
     5401 ccattgaaca gttggtaggc agggtaagcc aagctttgtt gagaggccat gtcttgccag
     5461 cgttgctgtt cttttttagc tgcctcttct ttgatacgat aggatttggc caagtcctcc
     5521 gtggtcatag ctgccatgat ttttaaaatg gcttggctgt cttgtggaag gaggtgtggg
     5581 tgagattcct tagggatcat catttctttt gcggttggaa ttaaaaaggt tagcatagtg
     5641 ttattgtagt aaagttactt gctttctgtc aagaaaacta gtcttgtcca aggatgacgt
     5701 tcacacagat accttcctcg tcttctgtcg tgaaagaaga agaatcttct tgtaaaatag
     5761 ctaggccgtg taatcttgcg tttttcagag tagctgaaaa aagcaggggc gtctcaatgt
     5821 gaatggtcaa gaaggctaag cctggggcac cttcttggtg ttttttgagg taaggggctg
     5881 accagtgatt gaatgcaagg tgatgatggt agttccctga agcaatccag ctagcacttg
     5941 gaatggtcat tttatcgcct aaatcaaaaa ccttttgata gagaagtgaa gaagccagag
     6001 catttttgac actcagatgg acatggccaa tgcgtgtgtc ttgagctagt aaaaagcgct
     6061 tggggatatc tgtcaactgc tccaatatgc ttttggtatc cgtgggctca gtcacaccaa
     6121 taatttgacc attgtcgcgg atatcccaat gctcaacagc tttgtcatga taaatttcaa
     6181 tcccattgtc ctcaggatcg ctgaggtaga tagcttcgct atagccgtga tcagctgctc
     6241 cttctaaaga gatacctctg gttaagaaat gattgagaac aagtcccaag ctatgacgat
     6301 caggaactaa aaaggctgtg tgataaagac cataagcctt atttgctggt aaaggtgttt
     6361 gtcgtaactc taagataacg gttttaccgt ctgtagtcaa ttgacgagat gttgtgtctt
     6421 gtgagagaac ctgtaaaccg ataatacttg tgtaaaaggt tgtcatcttt gctagatcgg
     6481 taacatttag agaaaccgtt cctaaagaga tggttgagtt gtaagggtat gtcatcctat
     6541 tacctccaaa aactaattaa atctatcata accaatcagt ttcaaaaaga aaagtaggca
     6601 caaataagtg gtataggcac taaaaagtaa ctcattta
//
LOCUS       DAUGFP010000061         6244 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.061, whole genome shotgun sequence.
ACCESSION   DAUGFP010000061 DAUGFP010000000
VERSION     DAUGFP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.061"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..603)
                     /locus_tag="VH663_001477"
     CDS             complement(<1..603)
                     /locus_tag="VH663_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEQ8174665.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIVLFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWF"
     gene            695..1042
                     /gene="ssrA"
                     /locus_tag="VH663_001478"
     tmRNA           695..1042
                     /gene="ssrA"
                     /locus_tag="VH663_001478"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1337..1963
                     /locus_tag="VH663_001479"
     CDS             1337..1963
                     /locus_tag="VH663_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEQ8174666.1"
                     /translation="MTYDKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRL
                     HQAAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPSQHDPKTAGEVTIDAYTW
                     QFDQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDN
                     YSQASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
     gene            complement(2220..4484)
                     /gene="glgP"
                     /locus_tag="VH663_001480"
     CDS             complement(2220..4484)
                     /gene="glgP"
                     /locus_tag="VH663_001480"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEQ8174667.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNSA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSTAEVEDVELEPSLGNGGL
                     GRLASCFIDSIASLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNYWIEDQSWLVPTNI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEDVDYGLIKDGISFDKTEIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNKDIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     CLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
     gene            complement(4519..6012)
                     /gene="malQ"
                     /locus_tag="VH663_001481"
     CDS             complement(4519..6012)
                     /gene="malQ"
                     /locus_tag="VH663_001481"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEQ8174668.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVADGYLQEADLVGVDFGSDPEVVDYA
                     QIFQARRPLLEKAVQAFLATKEGIKELAAFEAEASWLGDFAEFMSLKEHFQNKALQEW
                     DDRAIVKREEAALHHYRELLAKKITYHKVCQYFFYQQWSALKIYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            complement(6128..>6244)
                     /locus_tag="VH663_001482"
     CDS             complement(6128..>6244)
                     /locus_tag="VH663_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HEQ8174669.1"
                     /translation="MSFKQLLDIINSNEQNLSQTIFVPFSLKQRESVRDISH"
BASE COUNT         1987 a         1196 c         1070 g         1991 t
ORIGIN      
        1 gaaccaagat ataacatctt ttccaaaaat aaataaataa ctgtagccga taatagaaat
       61 tggtttagtt attaaaagaa tagttacaaa gcgctttaaa gacatgtccg tcaatcccgt
      121 aatcataacc ataatatcag ctggagaaac aggcgatgcc atacaaaaaa taaataattt
      181 ttcatatcct ggtgtctcca aacgcctttc atacttgtaa aatgttttgt catttacaaa
      241 gagtaagata aatttacgac cataagtctt aacaagcaag aagaggacta tactgccaat
      301 aatgatgcct acataattat aaataaaacc tgttacaggg ccaaagatta aaaaacctgc
      361 aactgtcgta aggcctccag gaatcacagg aaaaacaatc tgtattatct gcacgacaat
      421 aaaaacaaac ggcccccata atctatagcg ctgcactaag tctttaagag cattactatc
      481 gtttaatata cctagtctat acagccaaaa agcgaggaaa aaagtaccaa ttaatgcaat
      541 aattcctaaa attttgataa ttttttgcca taatatatag gcttttgaat tacgttttgt
      601 catagactaa tcataccata agctgtaaaa ttttgctatt taaaaaaagt tttgatagaa
      661 tagttagtgt aagaaattgg ttaattatct atttggggtt gttacggatt cgacaggcat
      721 tatgaggcat gttttgcgtc ccatcggcag atgtaaattg ccagttaaat ataactgcaa
      781 aaaatacaaa ctcttacgct ttagctgcct aaaaaccagc tagcgtgact tctacaagat
      841 tgcttgtgtc ctgttagaag tctcaaaata gcaagctacg gttacgaaat tgtctagttt
      901 cgtgacaaga gattgataga ctcgcaaact aatggcttga gttatgtgtc tttagtttgt
      961 taaatgaaga cataacctat ggacgtagac aaatatgttg gcaggtgttt ggacgtgggt
     1021 tcgactccca ccggctccat caatgcttac cgtaagtaat cataacttac taaaaccttg
     1081 ttacatcaag gttttttctt tttgtcttgt tcatgagttt ccgttatccc tatagccata
     1141 ccatcacgct gaccgatact ccatcaatac tactcaaaac cttgtcatat cagagctttt
     1201 tgaccatttt tttcatgaat atctaaaaaa aagaatcaaa atagtactaa attccccata
     1261 gcacatgcgt tatggggaat catagctata tcttgtttat aatttatgat ataatacagc
     1321 aaaaatagtt taggaaataa catatgacaa aaaacactta ctaacacttc ttctcatctc
     1381 tttttttact agctttttgg tagcttgttc aacaacgaaa gataaagagc ctcaaccgtc
     1441 tgattcagaa atcattactc cccgactaca ccaagccgct catcaagata aacgcgctaa
     1501 ctttgaaaaa attaaacttg cgactgttga ttcctcattt acaggaggaa caagccttga
     1561 agaacttatt tcactctttg gagagcctag ccaacatgat ccaaaaacag caggcgaagt
     1621 aacaatcgac gcttatactt ggcagtttga tcaagttact ctcactgtta atctttatca
     1681 aaatagtagt attgttaaaa ccatctctaa ttttaccttt gcaagagagt taggcttatc
     1741 gcaaaaggaa taccaacaat tacaaaaagg aatgtcttat gaagacgtta aaaagatctt
     1801 aacagaacct gataattata gccaagcgtc atctagtgat catcaaactt tgcaagcgat
     1861 ttgggttagt ggcttaaaga cagatacaag cggagctaat atttctctcg tttttgaaaa
     1921 taatcagtta acagaaatgt ctcaggtagg acttgaagaa taaaaaatgc gtaatatatt
     1981 ttgctaaaaa gattgatttc gatgtacacc gaaatcaatc tttttatatc attttaataa
     2041 aaaactttag tgtagtttct tataattacc tccatagatc gataaacaga cttgctttac
     2101 gcttaatgta ataattaacc taagtctgaa gaaaaatcat aagacagcag aaactactgt
     2161 cttataattt agtttaagca tgttacggta ccgctacccc acattgcaat tattattttt
     2221 tatcgtgaat gccaaatatc ttcgttatac tgctcaatgg tacggtcaga tgagaagaat
     2281 ccagcttttg cgatgttatg gaccactttt gtcatccata aatcttggtc ttcatagtct
     2341 gctaacattt tttctttcac ttcaatgtac tcttcaaggt caatcaaggt catgaaccag
     2401 tcttttgaaa tcaattcttt ataaagacga tcaagacatt cttcgttgcc aaatgctagc
     2461 aattctggac tgataataaa gttcactgct gctttaatag cagggtggtt atcatagtaa
     2521 tcttttgcta cataagaagc agttgcataa aggttgatga tggtatcaga atctttacca
     2581 aaggtgtaga tattttccat gcctgcaagc tcagcgattt ctacgttagc accgtccatt
     2641 gtaccaagtg ttaaagcacc gttaagcatg aatttcatat taccagttcc agaagcttct
     2701 ttggatgcta gtgaaatttg ctcagaaata tcagttgctg gaatcaagtg ctctgctact
     2761 gtcacgttgt aattttcaac tagatgcaca ttaaggtatg ggcttacttc agggtcattg
     2821 ttaatcaatt cagacaagca aaggatcaag tgaatgatgt cttgagcaat gatgtaagca
     2881 ggcgctgctt tacctccaaa gataacagtg attttacgtt ttggaaggtt gcctttttta
     2941 atttccaaat atttgtgaat cacgtaaaga gcattcatct gttgacgttt gtactcgtgg
     3001 aaacgtttga tttgggtatc aataatagaa tgttcatcaa gctcaatgtc tttattgtct
     3061 ttaaggtaac gtttaagagc taatttgttg ttgaatttga tttcagccaa tttagcatga
     3121 acagctttgt catctgcaaa ggccatcaat ttttctagtt ttgttgcgtc agtcaagtac
     3181 tcatcgccaa taagttcttt aatgtaatca gccaagtctt ggttagcaaa ttctagccaa
     3241 cgacggaaag taatcccgtt ggttttgttg ttgaattttt ctgggtaaag gtcatagaaa
     3301 gcttttaatt cgctgttttt caagatttct gtgtggagtg ctgctacccc attgacactt
     3361 gttgcaaaat gaatatccat atgggccatg tgcacacgac ctgattcgtc aataatttga
     3421 acagctgggt cagacacttc tgaacgaaca agagcatcca atttttcaat gatggttacc
     3481 aagtgtggca ctacttcgtt taagtaagca gttggccatt tttcaagggc ttctgcaaga
     3541 atagtgtggt tagtgtaacc aaccatattt ttcacaacag ctaccgcttc atcaaagtca
     3601 aagccatgtt tttcagtcaa gagacgaatt aattctggga tgaccattga tggatgcgtg
     3661 tcattaattt gcacgtaagc gtagtctgca agatcatgaa ggttagaacc acgttcgata
     3721 gcttcatcaa tgattaattg cgctgcattt gacaccataa agtactgttg gtaaatacgg
     3781 agcaattccc catttttatc agaatcgtct gggtagagga acaaggtcaa gtttttagca
     3841 atttccgttt tatcaaaaga aatgccatct ttgataagac cataatcaac atcctcgatg
     3901 tcaaataaat taaggtagtt tttagtgtca cgtttgtaac ccaaaacatc aatacgatca
     3961 agacgagatt ttaaggtaaa gtttttgaag ggcacatcat atgaaatgtt tgtaggaacc
     4021 aaccatgact ggtcttcaat ccagtagttt ggctcagctt cttgctcatt atgtttgaag
     4081 acttgcttaa acaacccaca atgatagttt aaaccaaccc cttcaccatt aatcccaaga
     4141 gatgcaatag aatcaatgaa acatgatgcc aaacgtccta aaccaccatt ccctagtgat
     4201 ggttctaatt caacatcttc gacttctgcg gtagatttgc cagcagctgc caattcttct
     4261 ttgatgtctt tgtaaattcc taggttaatc aggttgtttg atagtaattt accaatcaaa
     4321 aactctgctg agatatagta aactttgcgt ttagcagaat ttttagcctt gtggctagct
     4381 tcttctttga caaagtttaa taatgataga taaatctctt cattgctggc ttgtgtaagt
     4441 gattttccta gtttagtttc aacatattct gtaaagcgtg tcatgtcttc ctcttttcaa
     4501 taatgtaaca tttaaatatt atcttgcgcg accataaaga tctgttaaat aaataaggta
     4561 atctttgtga tcttggttca attcaccgtc aagcatacgc cattgccaat tacctccaat
     4621 tgtatttggc atattcatac gactcttacc atctttatct aataaatctt ggatgcaaag
     4681 aatggctgta tcacaaacag aagcaaagat tgttcttagc atggcttttg taatactttc
     4741 atctgcatgt ttgtgaagat agttatttac aaagtgtact tgttcttcac taagattgtc
     4801 ataccatccg ttaaccacct cgttatcatg agtccctgtg taagcgacac aattacggtc
     4861 atagtaatgt ggcaaatcta tactttgact cgttatatca aataaaccaa attctaatat
     4921 cttcatgcca ggaaagcctg ttgatgctaa tagctgctct gctttttcat caatataacc
     4981 aaggttttct gcgatgatag gaagctctcc taaggcctca cgaaccgcag aaaataatgc
     5041 tatacctggg gctgaggccc aatggccatt tctagcagtt ttatcaccag caggaatttc
     5101 ccaaaaatct gagaagcctt taaaatgatc gattcttagc tgatcatata gtttaaaact
     5161 ttcttgaata cgataaatcc accatgcaaa attagacttt tcatgagcag accaattgta
     5221 ggttggattt ccccacagct gaccatcttc actaaagcca tctgctggta cacctgcaat
     5281 aaacaatggc tttttatcac tatctacttt aaatagctca ggcatcgtcc aaacttctac
     5341 actgtccgca gaaacataaa taggcatatc accaataatt tctatccctt tatggttagc
     5401 gtaaattttc aaggctgacc attgttggta aaagaagtat tgacagactt tatgataggt
     5461 aatctttttt gcaagcaact cacggtaatg atgcaaggct gcttcttcgc gtttcacaat
     5521 tgctctgtca tcccattctt gtagggcctt attttgaaag tgttctttca atgacatgaa
     5581 ctcggcaaag tcacccaacc aactcgcttc tgcttcaaaa gctgccaatt ctttgatgcc
     5641 ttctttagtt gcaagaaagg cttggaccgc tttttctaat aaggggcgtc ttgcctgaaa
     5701 aatctgagca tagtcaacca cctcagggtc agaaccaaaa tcaacgccaa cgagatcagc
     5761 ttcttgcaag tagccgtctg caacaagtaa ttcaaaatca atgaaatgtg tgttcccagc
     5821 aatagctgaa aatgactgat aaggagaatc tccaaaactc gtcgttgtta aaggcagaat
     5881 ttgccaatag gtttgtttcg tctctgctag aaaatcaaca aattcaaaag ctgattttcc
     5941 aaaagtgcct atcccaaatt tcccaggcag ggaactaata tgcattaaga taccgcttgc
     6001 acgtttgttc atgatttttc tccttgtgaa atctattcat gatgagatta taacgcaagc
     6061 gcttgcgtaa gtcaaccagt ttcctaaaaa aagtaacatt ccttaaaaat gctacttttt
     6121 tttgctctta atgactaata tcacggacgc tttctctttg ttttaaacta aaaggaacaa
     6181 aaatagtctg actaagattc tgttcattgg aattgataat atcaagtaat tgcttaaaag
     6241 acat
//
LOCUS       DAUGFP010000062         6013 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.062, whole genome shotgun sequence.
ACCESSION   DAUGFP010000062 DAUGFP010000000
VERSION     DAUGFP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.062"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            201..812
                     /gene="rpsD"
                     /locus_tag="VH663_001483"
     CDS             201..812
                     /gene="rpsD"
                     /locus_tag="VH663_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEQ8174670.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(966..1499)
                     /locus_tag="VH663_001484"
     CDS             complement(966..1499)
                     /locus_tag="VH663_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ8174671.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFRTEELSQTEKEYQSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
     gene            1634..3907
                     /locus_tag="VH663_001485"
     CDS             1634..3907
                     /locus_tag="VH663_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEQ8174672.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKKGDYYMV
                     ITLPEDLSQRATTLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMTKLKESVSQNITKI
                     YTSAVFRSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEELSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     SLQSLSQEDQSKQLEQLKEAVAQIANQSNQALPGASSALTELSTGLAKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTAIGSGVIKLSEGTNALSSKSGELLDGSHQLSEGATKLADS
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(4023..5102)
                     /locus_tag="VH663_001486"
     CDS             complement(4023..5102)
                     /locus_tag="VH663_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEQ8174673.1"
                     /translation="MKNISRKCFITSVLCIILGGILLGAGYATGGLQDIKHQTAPKKV
                     IKTFDQITALDIDSSASTITVETGPVQRPTVTYYTHPKFIDPIVTTVTGKTLSLSQKP
                     KDVVITGGIEILGFTLNNSRQEKNYRSITITVPEKTSLNEVKGSNVPHTTLSNLTVQD
                     MQFDGNLTLLHTKVKKATITGMLEATKSQLTNLELKADYSFSNLTDSSVENGTISLGN
                     GQLTTKDTTLKAVNIQSLHPGGIEAERTTLENVTFTVSKSKEEEENDYYDNDAIFTAH
                     ALTLKGTNTITGGDIDVDITLTKAKAIAYRARTENGKVSLGSQLTPAKIGKESTSDVI
                     SYVAENKAATGNLTVNLNKGDITIK"
     gene            complement(5099..5686)
                     /locus_tag="VH663_001487"
     CDS             complement(5099..5686)
                     /locus_tag="VH663_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEQ8174674.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEAMEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGLAGILVAGVTLFESLTLIGSSIPAQAMGIGSALLCFG
                     GGLLIWIIATAILRFSGRAFVAFIKWISNKKGAQA"
     gene            complement(5673..5999)
                     /locus_tag="VH663_001488"
     CDS             complement(5673..5999)
                     /locus_tag="VH663_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEQ8174675.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
BASE COUNT         1799 a         1137 c         1176 g         1901 t
ORIGIN      
        1 tgatagatag actgacttta caaaacaact ttcttatggt aaactgttct ttgtgcgaaa
       61 taacagcaga aaaatatgaa gctcgtcaac aggtggttat ctattcaagt aacctttgct
      121 gtttaggcga aaaccccctg cacgaatcag gattttttaa accgttattt cctcaaaaaa
      181 tttagaggag gacatgtaat atgtcacgtt atactggtcc atcatggaaa caatcacgtc
      241 gccttggttt atcacttaca ggcacaggta aagaattggc acgtcgtaac tacgttcctg
      301 gtcaacacgg tccaaacaac cgtagcaaac tctcagaata tggtttgcag ttagctgaga
      361 aacaaaaact tcgtttctca tacggtttag gtgaaaaaca attccgtaac ttgttcgttc
      421 aagctacaaa aatcaaagaa ggaactcttg gtttcaactt tatggttctt ttagaacgtc
      481 gtcttgacaa cgttgtttac cgtctaggtt tagcaactac tcgtcgtcaa gcacgtcaat
      541 tcgttaacca cggtcacatc cttgttgacg gtaaacgcgt tgatatccct tcataccgcg
      601 ttgatccagg tcaagtgatt tcagttcgcg aaaaatcaat gaaagtacct gcaatccttg
      661 aagctgttga agctactctt gggcgtccag ctttcgtatc atttgatgct gaaaaacttg
      721 aaggttcatt gactcgcctt ccagaacgcg atgaaattaa cccagaaatc aatgaagcac
      781 ttgtcgttga attctataac aaaatgcttt aattttaaga ttaaacttac aaaaagccta
      841 tttaataggc tttttttgta tctcataaca aatctattct gatcccttca ttttactatg
      901 gcttctaaaa aagttatcac taaggctgat gacttgtaaa atgatacttt cgtaagggtt
      961 actatttagg tactcgtaag cattttgaga acgaattgtg tcatttcttg aggtgattct
     1021 tttttacctt tagcaatcca actttgacag acaccaaaaa aagcatgggc taagtagata
     1081 ctttggtatt ctttttccgt ttgagataac tcttcggtac ggaacttgtc ctgtaaatca
     1141 gtagtgatga gtaggcgaac tttattgatg ataaaggcct gtatttcttt agtaccgtta
     1201 gcagacaaaa gggaagagag aagctgttct ctttggagaa attcaaatac ttcaaggaag
     1261 gcttgttctt tattttgata ttttttctca aaaatatatt ctaatttgtg aaaaaaggtt
     1321 tgctgataat agtcaatcat ttcatattta tctttatagt gtgtgtaaaa actagagcga
     1381 ctaattccag ctcttttaga taatttaact gtggtaatat cgtcaaaact ttcggttttt
     1441 agtaacataa ccattgcttt taaaattgct tgcttcgtat tttctttacg gttgaccatc
     1501 taccatctct ccttttgaac acttttagac ataatgtcca aaagtgaatt tttttctctt
     1561 gtaaatttaa atttaaattg tataatatat tttatcataa tttttgaaca gtatgtccat
     1621 tttaggagaa aatatgttag aagaattgaa aacacttatt aaaaatccaa aattaatgat
     1681 tacaatgatt ggtgtggccc tagtgcctgc cttatataat ttatcctttc taggctcaat
     1741 gtgggatcct tatggtcggg tcaatgacct tcccattgct gttgttaatc atgataagcc
     1801 tgcaaagaga gctgataagt cattgacaat tgggaatgat atggtggaca agatgtctaa
     1861 aagtaaagat ttagagtatc attttgtctc agctaagcaa gctcaagagg gtcttaaaaa
     1921 aggtgattat tatatggtca ttactttacc cgaagatctt tctcagcgag caacaacctt
     1981 attaaatcct gaaccccaaa aactaactat ccgttaccaa actagtaaag gacatggaat
     2041 ggttgctgct aagatgggtg aaacagcgat gacaaaactg aaagagtctg tttcgcaaaa
     2101 cattacgaag atttacacct cagcagtttt taggagtatg acagacctcc aatcaggatt
     2161 aaaagaagcc tcagctggaa gtcaggcact tgcttcagga gcgaagaccg ctcaggcggg
     2221 gagtcaaaca ctttcgacga acttagcagc cttaacgggt gctagccaac agtttcaaca
     2281 aggtactggt cgattgacat caggtttgac tacctataca gatggtgtca accaagtcaa
     2341 gaatgggtta ggaacattat caacggacat ccccaattat ctgaatgggg tttctaggtt
     2401 atcccaggga gcttctcagc ttaatcaggg tctttcacag ttgacacaag caacaacact
     2461 ttctgacgag aaagctaagg gaattcaatc cttaattgta ggactaccag tcctaaatca
     2521 aggcattcag caactaaata cagagctatc aacattgcaa ccccctaacc ttaatgctga
     2581 tgagttaggt aatagcttag gagctatcgc tcaagctgcc aaacaagtca ttgctgaaga
     2641 gactgccgct cagaatgaag aattatcggc tctccaagct actagcgttt accaatcatt
     2701 aactgctgaa caacaaggag agttagctgc ggccctcagt caatctgata aaagtcagac
     2761 cgtatctgca gcccaaacta ttttaagttc tgttcaaact ttgtcaacaa gtttacagtc
     2821 tctctctcaa gaagatcagt caaaacagtt ggagcaactt aaggaagctg ttgcacagat
     2881 tgctaatcaa tccaatcaag ctttgccggg agcaagttct gctttaactg aattatcaac
     2941 gggattagca aaggtaaatg gtagcttaaa tcaacaagtt ctaccaggaa gtaatcaatt
     3001 gacaacagga ttagcacaat taaacaggta taatactgcc attggttctg gggtaataaa
     3061 actctcagaa ggtaccaatg ccttgtcatc caagtccgga gaattactag atggtagcca
     3121 tcaattatca gagggtgcta ctaaactagc tgatagtagt tctcaattga gtcagggtgg
     3181 tcatcaatta acgagcggat tgactgaatt atcaacagga ttatcaacct taaatggttc
     3241 cttagccaaa gcctctcagc agttatcgct tgtttctgtg actgataaaa atgctaaagc
     3301 tgtcgcaaaa cctcttgtgt taaatgagaa agacaaagat ggtgttaaga cgaatgggat
     3361 cgggatggca ccttatatga ttgctgtttc tctaatggtt gtggcccttt caaccaacgt
     3421 catttttgct aattctttat ctggtcgtcc ggtcaaagat aaatgggatt gggctaaaca
     3481 aaaatttgtt attaatggtt ttatttcgac tatgggatcc attgttctct acttagctat
     3541 tcaattatta gggtttgaag cccgttatgg tatggaaacc ttaggattta ttatgctaag
     3601 tggttggacg tttatggctc ttgtcacagc tttggtcggt tgggatgatc gatatggctc
     3661 ttttgcttct ttggtcatgt tattgcttca ggttggctct tcaggtggct cttaccccat
     3721 tgagttaagt ggagcattct tccaaaagtt acatcctttc ttaccaatga cttatgtggt
     3781 atctggttta cgacaaacca tttcattatc aggtcatatt ggagtagaag tgaaagtctt
     3841 aactggtttc ttactggcat ttatggtatt agcactactc atttatcgtc ctaagaaaac
     3901 agtctaaata aaataacctc aaaaaaaaca gcatctaagg cagttacact agatgctgtt
     3961 ttttgtctta ttttgacaat aacttgacaa tttctgtcaa gttttttatt gtcttgttaa
     4021 gatcatttga tagtaatgtc tcccttattg agattaaccg ttaaatttcc agtagctgct
     4081 ttattctcag ccacataaga aataacatct gaagttgatt ccttaccaat cttagctggt
     4141 gtcagctgtg agccaaggga gactttacca ttttcggtcc ttgctctgta ggcgatggcc
     4201 tttgcttttg tcaaggttat gtcaacatca atatcaccac cagtaatagt attagtccct
     4261 ttaagggtaa gggcatgagc ggtgaagata gcatcattgt catagtagtc gttctcctct
     4321 tcttctttgc ttttagaaac agtgaaggtc acattttcaa gggttgttct ctcggcttct
     4381 ataccaccag ggtgtaatga ttgaatattg acagctttta gagtggtatc tttagtagtt
     4441 agttgtccat ttcctaagct gatggtccca ttttccacac tagaatcagt caggtttgaa
     4501 aaagaatagt cagcttttaa ctcgagattt gttaactgac ttttagtggc ttccaacata
     4561 ccagtgatag tagctttctt gactttggta tgtaaaagag taagattgcc atcgaattgc
     4621 atatcttgga cagttagatt tgacaaagtg gtatgtggga cattacttcc cttaacttca
     4681 ttgaggctag ttttttcagg aacagtaatg gtaatagaac ggtagttctt ctcttgacga
     4741 ctattattga gagtaaaacc taaaatttca attccaccag tgatgacaac gtctttaggt
     4801 ttttgcgaaa gagatagggt cttacctgtt actgttgtga cgatagggtc aataaattta
     4861 ggatgtgtgt aataggtcac tgttggtctt tgaacgggtc ctgtctctac tgtaatagtt
     4921 gaggcagaac tgtcaatatc aagagcagtt atttgatcaa atgttttgat gacctttttg
     4981 ggagctgttt ggtgtttaat gtcctgaagt cctccagttg catagccagc ccctagaaga
     5041 atgcctccta gaataataca taacacactg gttataaagc atttgcgtga gatatttttc
     5101 atgcttgagc tcctttctta ttactgatcc acttaataaa ggcaacaaaa gcccgtcctg
     5161 aaaaacgaag gattgctgtg gcaataatcc aaataagaag accaccacca aaacacaaca
     5221 aggcgctgcc gatgcccata gcttgtgcag gaatagatga gccaatcaag gtcaagcttt
     5281 caaacagagt cactcctgcg acaagaatac ctgcaagccc aaaaatgtag gcggcaaaga
     5341 tagctgtgag cccgagcatt aggcaggcaa taataaagag aatcaacgcc agcaatacgg
     5401 gtaaagcaat cggagcagca aagagggaca aaatggttaa tccaataatt gttgcccgat
     5461 ttttgggagt tttctcggga gttgccaagt gttttcctag aacatttgaa atgatttcac
     5521 tggcagcttc cttaggattt cctaaatcgt cgataacctg gctttctttg tcaggaccag
     5581 cttcatcaaa atattcaata taatattcca tcgcttcatg gtagtcttct ctaggtagtt
     5641 tgcgcaagta tttatctagt tctgctagat actcagtcct tgtcatgtct taagctccct
     5701 tcaataatcc cgtctaaggc aaatttatag ctctgccatt ctttttttag aaaaatcagc
     5761 tgtgctctcc ccgaagaagt gacagcataa tatttacgct ttcgtccctg atgttcttga
     5821 ctataagttg tcaaaaaacc tgccttctcc aattttttaa gaataggata gagggtggat
     5881 tctttgatat tagccactag tttaatggtt tgactaatat cgtaaccgta agaatcgttt
     5941 ttttcgacaa tggctaaaat cagaaattca atcaaggtag ccgatactgg aaaatacatt
     6001 ttaggctcct ttc
//
LOCUS       DAUGFP010000063         5899 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.063, whole genome shotgun sequence.
ACCESSION   DAUGFP010000063 DAUGFP010000000
VERSION     DAUGFP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.063"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(311..1183)
                     /locus_tag="VH663_001489"
     CDS             complement(311..1183)
                     /locus_tag="VH663_001489"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEQ8174676.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEAESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            1407..2717
                     /locus_tag="VH663_001490"
     CDS             1407..2717
                     /locus_tag="VH663_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEQ8174677.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLIAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPVLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTIFPIIATISAQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            2795..3945
                     /locus_tag="VH663_001491"
                     /pseudo
     CDS             2795..3945
                     /locus_tag="VH663_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558257.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4074..4226
                     /locus_tag="VH663_001492"
                     /pseudo
     CDS             4074..4226
                     /locus_tag="VH663_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4273..4803)
                     /locus_tag="VH663_001493"
     CDS             complement(4273..4803)
                     /locus_tag="VH663_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEQ8174678.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(4796..>5899)
                     /locus_tag="VH663_001494"
     CDS             complement(4796..>5899)
                     /locus_tag="VH663_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HEQ8174679.1"
                     /translation="TFTSLEGRKKVSLYWVCLPMATRTALSSYEMFWYPWDDKTEDIW
                     VRPMPSQDYVINLENNSITTYRYKMNQEDLAKQFGRWYKQIHGNQKTVCILGNRASES
                     LHRYSGFINKKYGYQKECWITKQFKDVWTASPLYDWSVEDIWHAYYKFSYSYNELYDL
                     FYKAGLKPSQMRVASPFQDYAVDSLNLYRIIDQETWVKLLGRVQGVNFSNIYGRTKAM
                     GYKSIALPKGHSWKSYTQFLLSTLPVRLRNNYVRKFNKSIDFWHKTGGGLAEETINEL
                     IEKGYRIARNGISNYTTFKHSRVIFLDQIPDDTDDIVTTKDVPSWKRMCFCILKNDHI
                     CRTMGFGLTREQQKIVAFLKEKYKTSLKELESE"
BASE COUNT         1815 a         1276 c          958 g         1850 t
ORIGIN      
        1 cctttcaata attatttgtc caactttttg gggtcagtac actcctagtt aacgactttg
       61 attgatttta ttatgatgaa tcgtgatttc tgaaaacaaa cttttcttca tggatttggc
      121 gtggaacttt tacaataaaa acactaacct ctccctatcg ttttttcagg tgaatgcttg
      181 gaattggtat aataagcgga cacgaatcag gtgaaaacag ggaaagtatt tgaaaaggcg
      241 tttaaactat ctgacataaa actcgttcta gaggaaagac atcctttaaa aagttagttt
      301 attttgcaac ttagacatca aggtaggtta aacccttcat ggaaaaatcg agactcttag
      361 cactatgggt taaactacca ctggagacgt aatcaatcgc taaaccacga aaacggctaa
      421 tagtggtcat atcaatattt ccagaacatt caatccgaga acgtcctgca attagagtaa
      481 tggcctgttc aatctgttcc aatgacatat tatccaacat gataatatca acacccgccg
      541 ccgcagcttc ttcggcagca gcaaggcttt ccgcttccac ctcgaccatt ttcacaaaag
      601 gggcataggc acgcgcttga gcaatcgcct tttgaacact acctactgcc gcaatgtgat
      661 tgtcttttag catgatagca tctgataaat taaagcgatg attatagcca ccgccaactc
      721 tcacggcata tttctcaaaa agacgtaaat taggagtggt ttttcgagta tcaaatacct
      781 taatgcgatc atcgcctaag gcttctacat aagcagctgt catcgaagca atccctgata
      841 aatgttgtaa aaaattcaag gcaacgcgtt cacatgttaa gaggcttctc accgagccta
      901 tgatttctaa aaccaaatcg ccactagtca aacgatcccc atccttaaat tgatgaggat
      961 tctggaaggt cacctcggca tcaaataggg taaaaaccct ttgaaaaacg gttagccccg
     1021 ctaaaacacc agcttccttg gcaaaaagcg acaccttggc ttggccatga tgatcaaaaa
     1081 tggcattggt actgtaatct tcggaatgaa catcttctcg caaggctgct ttcaatgtat
     1141 catctatttg aaaaggggtt aaatcagttg aaatgattga catcactaag tcctctattt
     1201 ttaatatcac ttgtaagcac tagaggcaat tgctattaac ttccgtagga aatcacaaac
     1261 ctgaatacct cattttaaat cccaacaagt gtcaagacaa ttgtaatttt tttgtgtata
     1321 atagtcaagt accaatagga aacaggtggt cttttgagcg ccaaaattgc caacctattt
     1381 tcatattcta actaaaggac aattcaatga ttcatttaat aataattagc gccattgctc
     1441 tagccattgg aattggttac cgcaccaaaa tcaatattgg cctgctggct attgcttttt
     1501 cttacctcat cgcaaccact ctcatgggat taagccccaa agaacttctt catttttggc
     1561 caacctcact cttttttacc atttttagcg tctctctctt ttataacgtt gcaacaacta
     1621 acggcactct tgatgttttg gctcaacaca ttctctaccg cacacgcacc caccctaacg
     1681 ccctctacat gattttatac ctgatagcaa cccttttgtc tgctttaggt gctggatttt
     1741 tcactactat ggccgtttgc tgtcctctag cgattaccct ctgtcaaaaa gcggacaaac
     1801 accctttgat tggagctcaa gccgtcaatt ggggagcttc aggaggggct aatttgataa
     1861 ccagtggctc aggcattgtc tttcaaggcc tgtttaagca aatgggatgg gaagagcaag
     1921 ctttttcact tggcaatcat atctttatcg tcagcattat ctacccactc atcgtcttgc
     1981 ttttattatc ttgttacatc cgctacagta aaggaagaac aaactcatcc ttgactattg
     2041 accaaccacc agtcctcagc aaggtccaaa gacaaaccac cttgctcatg atcagcagca
     2101 tggttttggt ttggcttttt cccttgttgc atctcatttt cccaaacatc gcatggatag
     2161 ctacttatcg acaaacattt gatattggct ttgtctcaat actcatggtc tgcctcgcct
     2221 taaggctcaa attagggaag caagaagcca ttttggctaa ggttccctgg gctataatta
     2281 tcatgctctg cggcatgagc ttactaatgt ctctagcggt caagtcaggt ttagttacac
     2341 tgatagggca cctcataaca acaaccattc ctcatttctg gttacctctg ttcttttgtg
     2401 tgatagccgg agtaatgtca ctctttagtt ccaccctatc cgttgtagcc ccaactatct
     2461 ttcccatcat tgctaccatc agtgctcaaa gtcctcacat tgatattcgc ttactcacaa
     2521 cagccactat tattggcgct ctatctacta atatctctcc tttttcatca gctggttctc
     2581 tcattcaact ctcccttccc catatagagg aacgcagtct tgctttcaaa aaacaaattc
     2641 ttctgggtgt gcccatcagc ttaagtttgg cactcttgac catctggatt ctcatgctgc
     2701 tagcttcctt aagttagatt tattgtctaa cttaaggctg gacttggtat actaaagtag
     2761 acacaaaatt aagtgaaaac gaggaatagt cattatgcca agaaaaacct ttgataaagc
     2821 cttcaaactc tctgctgtaa aactcatcct tgaggaagag cagcctgtaa aaatggttag
     2881 ttcaacttta gaaattcatc ccaatagtct ttatcaatgg attcaagaat atgaaaaata
     2941 tggagaaagt gcgttcccag gacatgggag cgcacttcgt catgctcaat ttgagataaa
     3001 aaaacttgaa aaagagcgca aactgctaca ggaggaatta gctcttctaa aaaagttcca
     3061 ggtcttcttg aagccaaacc ggaaataaaa tttcagtttc tgaagaaaaa tagtgttagc
     3121 ttgaatagtc atcacgcctg tcaaactctt caagtctctc gttcaggttt ctatgcttat
     3181 ttaaaacgtc gtccctcttc aaggcatgtt gagaatgaag ctttaaagga aatgattaaa
     3241 gccattttct atgaacataa agagcgttac ggaagtgtac gaattaccca agaactttgt
     3301 agacgtggca tccatgtcaa ttataaatgt gttggtagac tccttcatca gctaggtctc
     3361 tatgctaaag gaagtcgata tcaatataaa tactataacc gtcgacgttc ttcattaact
     3421 cgtcccaatc ttgttaatca atgtttccaa gcgactggta agaataaatt atggctagga
     3481 gacttaacct acattcctac acaagaagga atactttatg tgtctgtctt gatagacgtt
     3541 tatagtagaa agattgttgg ttgggcaatg ggccgacgta tgcaagacaa gttggtaaca
     3601 gaagctttca aacaagctta taatagagaa aagcctaaag aaggggtaat cgtccataca
     3661 gatcagggtt ctcaatatac cggagcatga tttcaggacc tacttagaca gaaaaaatgt
     3721 aagtccagta tgaatcgaaa aggtaatcca tacgataatg cactcatgga atccttttat
     3781 aaaacattaa aaagagagct tgtcaatgat gctcatttcg caactattaa gcaagctcag
     3841 cttgagattt ttaaatatag tgagacttac tataacccca aacgtctgca ttcagcttta
     3901 gggtatctct cacctgttga atttgaaaaa atagttactc attagcttaa ctttatgtct
     3961 atttttcttg acaagtccag ggtatgaagt ccccttatac acatactttc aaaacagctc
     4021 tcaatcctct gtccgctaca aaaatacgtc accgctgccc tccagctgct tcattcaacg
     4081 acaaacttga agccacaaac agactcatca agggcattaa gtacaatgcc tttagatttc
     4141 agaactttga caactttaaa accaaaatcc tcatcgcttt gaacatccaa aaagagagaa
     4201 ccaatctgat tctctctcgt tgttaatgat aagtcaccca ctacagttga caatgagtct
     4261 cttacttcct gtttactcat ttaatagctc ttctggttcc caagattgat taaactcatg
     4321 gtccttaaaa agagaagcca atcctgttat ttgtttcagt cttaaaattt catctttatc
     4381 cattcctaaa tgttttgcaa tccagttatc acttcgccca cactcatgaa gatcctttac
     4441 aatctgactc atcaaatcaa catcatgact tcctctagct ctattatgtc gaatggtaga
     4501 agccattcga tagtctaatg acttatcaat aacagaaaca ggtaatctcc cactttccct
     4561 ttcataaata tctgaataat ctagcattat acgatatcta tgaaaaccat caacaatact
     4621 ataccgatcc tcttccttat cgtaatagca aacaatcggc atagtataac catcagattt
     4681 tatactatca tacaataatt tcatttcagg cggagcaact gaattcggat tataatcatt
     4741 tggagaaatc ttttcaattg gaattgattt aatctcatag actggactaa taaaattact
     4801 cactttctaa ctcctttaaa ctcgtcttat atttctcttt taaaaaggct actatttttt
     4861 gttgctctct tgtcaaacca aaccccatag ttcgacaaat atgatcattt tttaaaatac
     4921 agaaacacat tcgtttccag cttggtacat cctttgttgt tacaatatcg tctgtatcat
     4981 caggaatttg atctaaaaaa ataacccttg aatgtttaaa agtggtataa tttgaaatac
     5041 catttctagc aatacgatat cctttttcaa ttagttcatt aattgtttcc tccgcaagtc
     5101 ctccgcctgt tttatgccaa aagtctattg atttattaaa ttttctaaca taattatttc
     5161 ttaacctcac tggtagcgtt gataaaagaa actgagtata cgacttccac gaatgaccct
     5221 ttggtaaagc aattgattta tatcccattg ctttagtacg tccataaata ttcgaaaagt
     5281 taactccttg aacacgtccc aaaagcttta cccaagtttc ttgatctatt attctataaa
     5341 gatttaggct atcaacagca tagtcttgaa aaggagatgc aactctcatt tgtgatggtt
     5401 ttaaacccgc tttatagaat agatcataaa gttcgttata agaataagaa aacttatagt
     5461 aagcatgcca tatgtcttcc actgaccaat catataaggg agatgctgtc cagacatctt
     5521 tgaattgttt ggtaatccag cattcctttt gataaccata ctttttattg ataaaaccac
     5581 tatatctgtg taaggattct gaagcacggt ttcctaatat acaaactgtt ttttgatttc
     5641 cgtgaatttg tttataccac ctaccaaatt gtttcgctaa atcctcctga ttcattttat
     5701 aacgataagt agtaattgaa ttattttcca aattaataac ataatcttga gatggcattg
     5761 gtcttaccca gatatcttct gttttgtcat cccacggata ccaaaacatt tcataactac
     5821 taagtgcagt tctagtagcc atcggtaagc acacccaata caaagaaact ttttttctac
     5881 cttctaaaga agtgaatgt
//
LOCUS       DAUGFP010000064         5584 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.064, whole genome shotgun sequence.
ACCESSION   DAUGFP010000064 DAUGFP010000000
VERSION     DAUGFP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.064"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1248
                     /locus_tag="VH663_001495"
     CDS             <1..1248
                     /locus_tag="VH663_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ8174680.1"
                     /translation="DLYYVKLENGLTVYFIKKIGFLEKTAMLTVGFGSLDNKLTVDDE
                     SRDAPAGIAHFLEHKLFEDESGGDISLKFTQLGAETNAFTTFNQTSFFFSTASKFQEN
                     LELLQYFVLSANITDESVSREKKIIGQEIDMYQDDADYRAYSGILQNLFPKTSLANDI
                     AGSKASIQKITKILLETHHTYFYQPTNMSLFIVGDIDIDETFLAIQRFQTTLSYPDRK
                     RVTVDPLHYYPVIKSSSVDMDVTTAKLVVGFRGYLTLTQHSLLTYRIALKLFLSMLIG
                     WTSKIYHTLYEDGKIDDSFDVDVEIHHHFQFVLISLDTPEPIAMSNYIRQKLATIKIS
                     KEFTNEHLNLLKKEMYGDFIQSLDSIEHLTHQFSLYLSDSDKETYFDIPKIIERLTLK
                     DVVTIGKAFFEKADASDFTVFPK"
     gene            1298..2323
                     /locus_tag="VH663_001496"
     CDS             1298..2323
                     /locus_tag="VH663_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ8174681.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIAILGTVAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            2337..2879
                     /gene="pgsA"
                     /locus_tag="VH663_001497"
     CDS             2337..2879
                     /gene="pgsA"
                     /locus_tag="VH663_001497"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEQ8174682.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            2881..3720
                     /locus_tag="VH663_001498"
     CDS             2881..3720
                     /locus_tag="VH663_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ8174683.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            3696..4538
                     /locus_tag="VH663_001499"
     CDS             3696..4538
                     /locus_tag="VH663_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ8174684.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELKEV
                     LKHG"
     gene            4531..5331
                     /locus_tag="VH663_001500"
     CDS             4531..5331
                     /locus_tag="VH663_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ8174685.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
BASE COUNT         1818 a          891 c         1035 g         1840 t
ORIGIN      
        1 gacctttact atgtaaaatt agaaaatggt ttaactgttt attttataaa aaaaataggg
       61 tttttagaaa aaacagctat gttgacagta ggtttcggtt ctcttgacaa taagttgaca
      121 gtcgatgacg aatcacgaga tgctccagca ggtattgcac actttttaga acataaactc
      181 tttgaagatg aatcaggtgg agatatttca ttaaagttta cacagctagg agcagagaca
      241 aatgccttta caactttcaa ccaaacgagt ttcttctttt caacagctag taaatttcag
      301 gaaaatttgg aactccttca atattttgtt ctttctgcta atataacaga tgaatctgtt
      361 agtagagaaa aaaaaataat cgggcaagaa attgatatgt atcaagatga tgctgactac
      421 cgtgcatata gtggaatcct tcagaactta tttccaaaaa caagtttagc taatgatatt
      481 gctggctcaa aagcatccat tcaaaagatc acgaaaatat tattggaaac gcatcatact
      541 tatttttacc aacctactaa tatgagtcta ttcattgtcg gtgatattga tattgacgaa
      601 acttttttag ctattcaaag gtttcaaacc acattatcct atccagatag aaaaagggta
      661 actgttgatc ctttacacta ctatcctgtc attaaatcat cttctgttga catggatgtt
      721 actactgcca agctggttgt tggttttcga gggtatctga ccttaactca gcattcacta
      781 ttaacgtatc gtattgcctt gaagctattt ttatcaatgc ttataggttg gacttctaaa
      841 atctatcata cgctatatga agatggtaaa atagacgatt catttgatgt ggatgttgaa
      901 attcatcatc attttcaatt tgtattaata tcacttgata caccagagcc tatcgcaatg
      961 tctaactata tccgtcaaaa attagccact ataaagatat caaaagagtt tacaaatgag
     1021 cacttaaatc tattaaaaaa agaaatgtat ggtgacttta ttcagagttt agactctata
     1081 gaacatttaa cccatcagtt cagtctatac ctgtctgatt ctgataaaga aacatatttt
     1141 gatattccaa aaattataga aagactcact ctaaaagatg ttgttacaat tggaaaagca
     1201 ttttttgaaa aagctgatgc ttcagacttc acagtcttcc caaaatagat gaaaatctgt
     1261 tataataggg ttactatatt atttttgagg tacctgtatg agtgaaaaaa gtcttggtga
     1321 agtgttaaga gaatcgcgtg ttggaaaaaa tatcacacta gatgatatag aattgaagac
     1381 agggatttct tcccattact tgctggcaat ggaattggat cagtttaaaa ttattccgga
     1441 agaaaaattt gatcaatttt taaaagaata tgctgacatt gttgagttag atttcaatac
     1501 tttaaaaaga cgataccgtt atcaagttaa ttccaaaaaa gacagtgata taaaatctgt
     1561 aactgagatt gttgaagaga agttaagtaa gaaacgatta caagagaagt tgttatcaca
     1621 tcagagcatg gggtatggtc aaccgttaga agtttctaaa aacaaagaaa ccttagttat
     1681 ccctgtaaag actgttggtt tagatagcaa atcgccacta aaaacgatat cgcctgttag
     1741 ccaatttaaa tcagataaca ggttaattcc taaaaaaaat gtgtcacgta tgaggcgaaa
     1801 tagcactgac agtcgttcta aaacgtcact atttcccata gctatattag gaacggttgc
     1861 ttttgcaatt atcagtgtta tcattttagg tgtttggcaa caattcgaaa agagtcaaag
     1921 agctaaagaa gctgaagttg ctttgcttaa agcctctgag cagtctacta acggtttaaa
     1981 aaataattca aatgatcgca aaactcaagt cacaactgag gggtcgggta attatttaat
     2041 agcgactgtt actaaatcta aagagaccgt tgatatcagt gtgtctttaa ctgaggcaca
     2101 aagtagttgg atttctctaa caaattcaga aattggtgaa ggtggtataa cattgactca
     2161 agactcgcca acttatacag cgactttacc tgcagaaata acagaatcat tgttgacact
     2221 tggtgtgaca aaaggtgtat ctgtaacaat tgatggacaa cctgttgatc tatcaccttt
     2281 aacaagtact gacttaagtt atatcacgtt taagatccaa taattaaggt agactaatga
     2341 taaaaaaaga aaatattcct aatctattaa ctcttgttcg aatcgcaatg attcctttct
     2401 ttctttttat tacttcctca tctaataagg tggggtggca tatttttgca gctgtgattt
     2461 ttgcaattgc cagttttacc gattatctag atgggtatct tgcgcgtaag tggcatgtag
     2521 ccagtaattt tggaaagttt gccgatcctc tagcagataa gatgcttgtc atgagtgcct
     2581 ttatcatgtt agttggactt ggcttagttc ctgcgtgggt gtcagctgtt attatttgcc
     2641 gagaattggc agtaactggt cttcgattac tacttgttga aaccggagga aaggtccttg
     2701 cagctgctat gccgggaaaa atcaaaacag cgacacaaat gttgtctatt attttattac
     2761 tttgccattg gatattccta ggaaacgtct tactctatat tgctcttttt ttcactattt
     2821 attctggata tgattatttt aaaggagcaa gctttctttt taaggatacg tttaaataac
     2881 atgtcagcta ttattgaact taaaaaagtt acatttaatt accataaaga ccaagaaaaa
     2941 ccaacattag atggcgtatc gtttcatgtg aaacaaggtg agtggctgtc tatcattggc
     3001 cataacggat ctggtaaatc aacgactatt cgattaattg atggattatt ggaaccagaa
     3061 tcagggtcta ttattgttga tggagatcta ctaaccataa ctaatgtttg ggaaattcgt
     3121 cataaaattg gtatggtctt tcaaaaccct gataatcagt ttgttggagc aactgttgaa
     3181 gatgatgttg cttttgggct tgaaaataaa ggtattgcac atgaagatat aaaagagagg
     3241 gttaatcatg ctttagagtt agtcggcatg cagaacttta aagaaaaaga accagcccgt
     3301 ttatctggtg gccaaaaaca gcgcgtagct attgcaggtg cagttgctat gaagcctaaa
     3361 attattattt tagatgaagc tactagtatg cttgacccta aaggacgatt agagttaatc
     3421 aaaactataa aaaacatccg tgacgactac cagctgactg ttatttccat tactcatgac
     3481 ttagatgaag ttgctcttag tgatagagtt ttagtgatga aagatggtca agtggaatca
     3541 acatcaacac cagaacaatt atttgcaaga ggggatgaac tactccaatt aggtcttgat
     3601 atccctttta caacatctgt tgtacagatg cttcaagaag aaggttatcc tgttgactat
     3661 ggatatctca cagaaaagga attagaaaat cagttatgtc aattaatctc caaaatgtaa
     3721 gttatactta tcaagcaggg actccttttg aagggcgtgc cctttttaac attaatttag
     3781 atattttaga tggttcttat acggctttta tcggacatac tggatccggg aaatcgacca
     3841 ttatgcaatt gctaaatggt ttacacgttc cgacaacggg aattgtcagc gtagataaac
     3901 aagatataac gaatcactct aaaaataaag agatcaaatc tattcgaaaa catgtaggat
     3961 tagtttttca atttccagaa agtcaactat ttgaagagac agttttgaaa gatgttgctt
     4021 ttggccctca aaattttggg gtctctccgg aggaagcaga agctttagcg cgtgaaaaac
     4081 tagctcttgt tgggatttca gaaaatcttt ttgaaaagaa tccatttgaa ctctcagggg
     4141 gtcaaatgcg tcgtgttgct attgcgggta tccttgctat gcagccaaag gttttagttt
     4201 tagatgaacc gactgctggt cttgatccta aagggcgtaa agaattaatg actattttta
     4261 aaaagttaca ccaatctggc atgactattg ttttagtaac ccatctgatg gatgatgtgg
     4321 ccaattacgc agattttgta tatgttcttg acaaaggtaa aattatttta tcaggaaaac
     4381 ccaaaacaat ttttcaacaa gtcagtttgc ttgagaaaaa acagttagga gttcctaagg
     4441 tgactaaact tgctcaaagg cttgttgata gaggaatacc tatctcttct ttgcctatta
     4501 ccttagagga gctaaaagag gtactaaaac atggataagc tgattcttgg tcgttatatt
     4561 cccggagatt ctctcattca tcgtcttgac ccaagaagta aattattagc gatgattatt
     4621 tatattgtca ttatcttttg ggctaacaat gttgtgacaa atttgttgat gttgactttt
     4681 actttagcag ttgttttttt gtcaaaaatc aagttgtctt tctttttaaa tggggttaaa
     4741 ccaatgattg gtattatcct cttcacgact ttatttcaga tgtttttctc acaaggagga
     4801 aaagtaattt ttagttggtg gttcattagc ataactgatt taggattgag ccaagctatt
     4861 ttaattttta tgcgtttcgt tctaattatt ttcttttcaa cgctgttaac gttgacgaca
     4921 acacctttaa gcctatctga tgcagttgaa tcattattaa aacctttaac aagatttaaa
     4981 gttccagctc acgagatcgg actaatgctt tctttaagtt tacgttttgt gcctacttta
     5041 atggatgata ccacgcgtat tatgaatgca caaagggccc gtggagttga ttttggagag
     5101 ggaaatttaa ttcaaaaagt taaatccatt attcctattt taatccctct tttcgcttca
     5161 agctttaaaa gagccgacgc attagctatt gctatggaag ctagaggtta tcagggagga
     5221 gaagggcgaa ctaaatatcg tcaattagac tggcagctaa aagatagttt agctattgga
     5281 atagtttctt tattgggttt gctacttttt ttcttgaaaa cctctttgta atatttttta
     5341 gcacaaaact tttctttatt ataagaagtg aattgtacct caaaagttag atacaacatc
     5401 taatgattga ggtacttttc ttcatttgag gaattgaaaa ccgctatctc aactatatta
     5461 atcctcccaa taataacaaa tcgaacgaaa tctaaaagga ctgagtcctg ttcaatacag
     5521 aactaaattc ttagtttaat gatattatct cactttttaa tgtagctaag aagaagagtt
     5581 ttat
//
LOCUS       DAUGFP010000065         5539 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.065, whole genome shotgun sequence.
ACCESSION   DAUGFP010000065 DAUGFP010000000
VERSION     DAUGFP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.065"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..1234)
                     /locus_tag="VH663_001501"
     CDS             complement(<1..1234)
                     /locus_tag="VH663_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEQ8174686.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLSLSVAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKSIFSGQQVDIDPLGAIVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVAVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMIPEEEIFDNVAKNLYRYEKLILSKVPDYDHYIAKSFQLIDANGQ
                     TVNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQHTSIWRRHESWSLLFHQ
                     ITPIAKRQLHPTHYRIVKM"
     gene            complement(1354..2676)
                     /locus_tag="VH663_001502"
     CDS             complement(1354..2676)
                     /locus_tag="VH663_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEQ8174687.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNESVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFVLM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     regulatory      complement(2804..2890)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3205..5523
                     /gene="pcrA"
                     /locus_tag="VH663_001503"
     CDS             3205..5523
                     /gene="pcrA"
                     /locus_tag="VH663_001503"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEQ8174688.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALALNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSAEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
BASE COUNT         1837 a         1078 c         1129 g         1495 t
ORIGIN      
        1 acatttttac aatacggtag tgtgtgggat gtaattgtct cttagcgata ggggtaattt
       61 ggtggaagag taaggaccaa gattcatgac gcctccaaat actggtatgt tgattaccat
      121 ttaattggta agttaccaac atcgtttttt gactaatgga ttcaatctga aaatggttga
      181 agttacttgg ataataaatt tcttggttca aaaattgttc atagttaact gtttggccat
      241 tcgcatcaat cagttggaaa gacttagcaa tgtagtggtc atagtcagga accttactca
      301 aaattaattt ttcgtagcgg tagagatttt tggcgacatt atcaaaaatc tcttcttcgg
      361 gaatcatggc aggctcaacg tgaatgtcaa tgtcataaat agaaaattgg tcactcaata
      421 actgctccac tttctctgta atagagtgac tttcatagac tgaaagatca ggattcattt
      481 caagtacaat atcaagatag acattgctac cataggtcct agctcgttga gacttaacgg
      541 cgacaatttt agggatttct aaaatggctt tttcgtattt tttcaaatga cggctatcaa
      601 atccatcaga taaactaaac gaactttcca taaagatatc aaaagctgtt ttaagaataa
      661 agaacgtaat gatcatagca gctatatgat cgataactgg taaatgcaaa gaagctgcta
      721 taatagcaat tgatgttccg atagaagtaa cagcatcagc tagattatcc ttagaagcag
      781 cgactaaggc actggatttt acacgtttgg aaagacgttt gttaaagaca taaaccccta
      841 acataacaaa agctgaaacg ataccgacaa tcgccccaag agggtcaata tcaacttgct
      901 gaccactaaa gatactttta attgtgtgaa tcagtacttg gaaacctaca agaaacataa
      961 taaaagaagt gacaaggctg gataagtctt caattttcca atgaccaaat ttatgattgg
     1021 catcggctgg ttggctagct aagtgaagac caataagcag ggctacattt cccacaatat
     1081 ccgataaatt gttaaatcca tcagcaataa gggaacttgc atttaacaaa taaccagcta
     1141 ataatttggc aacactaagc gacaagtaga caataatact aacgattggg ccctttctgg
     1201 ctagttttaa atttgcaatc ggatcctgtg tcataataac tcctttgatt tcaactttat
     1261 attataccat aaaaaggggc ttaagaaaaa agcctagcgt cttgaacttg gcagaagaag
     1321 actaggtata actgggtagt actaacgtca tgtttactta ataaagtaat gcttggtttc
     1381 taaaataaca actggcgaga gagctaggag ggcaatcagg ttaggaagtg ccattaaacc
     1441 attgacaata tcagccaaaa cccaaatcag ttctaactta aggaagcctc ctaggcctac
     1501 cattaagaca aagacaatac ggaagtaggt gagatgtgta atgccaaaga ggaattcaaa
     1561 gcagcgttct ccataataac tccatcctaa aatcgttgta aaggcgaaga gaactaggga
     1621 aaaggttagg ccaaaagtcc ctaaatttcc aaacacagtg gcaaaggcag attgggtcag
     1681 aggagcccct tcgagctgcc cagtccattg gcctgttact aaaatagata ggccggttag
     1741 ggtacagata ataatcgtat caataaaagt tcctgtcatg gagatcaggc cctgctccac
     1801 ggattcattg gttttagctg ctgctgcagc aataggtgca gatcctagtc cagactcatt
     1861 ggagaagaca ccgcgtgcaa ttcctttttg aatggcatcc ttcatgagac ttccagcaaa
     1921 gcctccaata gcagctgtcg gagtgaaggc tgattgaaag actaaacgaa taacaggaag
     1981 tagctgctgg tagtgactaa aaataactgc taaactagat agaatataaa aaatagccat
     2041 aaaaggaacg actttttcag ccactttgga aatggaatga atgccaccaa aaataatggc
     2101 agccacaaag atagctaaaa caatactgac catttgtggg gataagccaa agctatgacc
     2161 taaagacgag gtgatcgagt tcacttgagc aaaggttcct attccaaata gggcaactag
     2221 gatccctgaa cccgcaaaaa gaatggctaa gggtttccat tttgtcccca tgccattaac
     2281 aatatagtac ataggaccac cagaaatatg accgttggca tcctttgtac ggtatttaat
     2341 cgctagtacc ccttcagcgt acttagtggc cattccaaaa aaagcggcaa cccacatcca
     2401 aaagagcgct ccaggaccac cagacttgat agcagtggca accccaacaa tgttaccagt
     2461 tccgacagta gcggcaaggg cagttgcaag agcagcaaag gatgaaatat ctccatgtcc
     2521 ttcgtcatct gaaaaaatga gtttaaaggc tcttggtagt tttaagattt gaattaatcc
     2581 taaacgactg gtaaggtaaa tccccgtccc aaccaataaa attaagaggg gcggtcccca
     2641 aacaaggtta tcaattaatt taacgagtgc tatcatcttt ctctcctaaa agtcatcgaa
     2701 gacgccaaaa gaaaaaagct atctctttct ttgagacaac ttctgaagca atgataaaac
     2761 agcaaacaca aaaaagacta agactgttct taatggtgtt tgtattttac cttctgtcct
     2821 tttgcctgag agattgagca gtatgccttg ccccttcggc gcctaataag gtctctccag
     2881 aagtccgtcc atcaaacagt cccgttcaaa aactgaacac ctgagagtat aactccttcg
     2941 gcgaaaaaaa ttcttctcct gcatgacatc attcggttta ttaaattagg actatcatac
     3001 ctcaaaaaac gaatgaagtc aaccaaaata gcaaaaaaat gaggaatgct ttaagaaatc
     3061 gctattattt ttaaatatct gaacctaaac tagaaaaaag gaatctcttt acttttgggg
     3121 tcttctttgc tatttttctt ggaaacatga tataatttta aggttgataa aagagatgtc
     3181 actgaacgag attaaggaga aagcatgaat cctttattaa atggaatgaa tgatcggcag
     3241 gcacaagctg ttcaaacaac tgaaggcccc ttattgatta tggcgggggc tggttctggt
     3301 aaaacaaggg tactgaccca tcgtattgcc tatttaattg atgaaaaatt tgtcaatcct
     3361 tggaatattt tagctattac ctttactaat aaggctgcgc gtgaaatgaa agaaagagcc
     3421 ttggccttaa atccagctac taaagatacc ttaattgcta cctttcactc catgtgtgta
     3481 cggattctgc gtcgtgaggc tgatcacatt ggttacaacc gaaacttcac aatcgtggat
     3541 ccaggtgagc aacgaacgtt gatgaagcgc attttaaaac aattaaatat agaccctaaa
     3601 aagtggaatg aacgttctat tttaggaact atttcaaacg ctaaaaatga tttgcttgat
     3661 gaaaagggat acgaggcaca ggctgctgat atgtatagtc aaatcgtagc gaggtgttac
     3721 aaagcttatc aggaagaatt acgacgtagc gaagcacttg actttgatga tttaatcatg
     3781 atgacgctac gcctgtttga cgctaatcct gacgtgttag cttattacca gcagcgttac
     3841 caatacattc atgtggatga gtaccaagat accaaccatg ctcaatatca gctaattaaa
     3901 ttgctagctt ctcgctttaa aaatatttgc gttgttggag acgctgatca gtctatctat
     3961 ggttggcggg gagctgacat gcaaaatatt cttgattttg aaaaagatta cccagatgct
     4021 aaggtagtgc ttttagaaga aaactatcgc tctaccaaga aaattctgca agcagctaac
     4081 gacgtgatta acaataaccg taatcgccgt cctaagaaac tttggacaca aaatgcggat
     4141 ggggaacaac tggtttatta cagggcaaat gatgagcgag atgaggcagt atttgtggcc
     4201 tcaaccatta gcaatatgag tcaagagctt ggaaaaaact tcaaagattt cgctgtttta
     4261 taccgtacca atgcccaatc tcggaccatt gaagaagcac tcttaaaatc caatattcct
     4321 tacaccatgg ttggaggcac caagttttac agccgtaaag aaattcggga tttgattgcc
     4381 tatttaacca ttgttgctaa ccctgctgat aatatttctt ttgagcggat tgtgaatgag
     4441 cctaaacgag gtgttggtcc aggaacgctt gataaactgc gtcagtttgc ctatgagagt
     4501 gaccagtctt tattagaagc agcttcaaat ctattgatgt cccccttgaa aggtaaagca
     4561 gcacaagcta tcatggactt ggccaatatt ctcggtcagc tacgtcaaga cctagatcag
     4621 atgagtatca ccgacttagc agaagctttg ttagaaaaaa caggctattt agatagctta
     4681 cgcctgcaaa atacattgga aagccaagcc cgtatcgaaa atatcgaaga attcttatct
     4741 gttaccaaaa attttgacga gtcaagtgcc tctcaagaag aagacgaaac cggtgttgat
     4801 cgcttgggac gttttttaaa cgatttagca ctgattgctg atacagatga ttcccaagca
     4861 gaagctgctg aagtaacctt aatgacccta catgctgcca aaggcttgga gttccctgtt
     4921 gttttcttaa ttggtatgga agaaggtgtc tttcccttat cacgtgcctc tgaagatcca
     4981 gatgaattgg aagaagaaag acgtttagct tatgtcggaa tcacaagagc tgaagaagtt
     5041 ctctttatga ccaatgccaa caccagaacc ctctttggta aaagtagtta taaccgtcca
     5101 acacgcttct tgaaagaaat ttcagaggaa ctactctctt ataaaggatt ggcaagacca
     5161 gcacaatctt catttggtgt gcgtttttct actgaaacac acacacagtt tggacaaggg
     5221 atgagcttat cagaagcctt acaagctcga aaggcccagg ctcaagtaag acaatcagca
     5281 cagccaatgc aggcacatac gataccatca gcttcaacta gctcagtttt accgtttggt
     5341 tctaatagct ctgcagaaga ggtcacttgg cagattggcg atatcgctca ccataaaaaa
     5401 tggggagacg ggactgtgct tgaggtttca ggctccggaa aaacgatgga attgaaaatc
     5461 aaatttccag aagttggttt aaagaaatta ctagcaagtg ttgctcctat tgagaaaaaa
     5521 taagaaaaaa atgagcttc
//
LOCUS       DAUGFP010000066         5474 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.066, whole genome shotgun sequence.
ACCESSION   DAUGFP010000066 DAUGFP010000000
VERSION     DAUGFP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.066"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(113..1255)
                     /locus_tag="VH663_001504"
     CDS             complement(113..1255)
                     /locus_tag="VH663_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEQ8174689.1"
                     /translation="MTINRVLKDDNLLQYTEKEFIEDMKILVAIDSFKGSVTSPELNT
                     SVAQALLSVDKQLVIETRAIADGGEGSLAALSQTVAGRWHQVKTIDLLRRPIKVAYYR
                     HAKQAFIESASIIGIDKITPNSVTYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTS
                     DGGKGFLESLNYDFMTGRSYLDTLASPVTLLGLTDVTNPYHGPQGFAAVFGPQKGGSL
                     SQIEETDQIASNFAKKVFCQTTIDLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAEL
                     LNLDNSLQSCDLVITGEGCLDTQSQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLA
                     AEDFLAVFSIQQQPISLEAAIDKTTTLSNIKILAANLMLLIAQFNK"
     gene            1536..4514
                     /locus_tag="VH663_001505"
     CDS             1536..4514
                     /locus_tag="VH663_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEQ8174690.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDSQTTSAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVVTTIQKLNAMISQMESYDTPKFKKLKERLEHLNVV
                     FVVDECHRAVTPERQRHLTNTFRNSRWYGFTGTPIFMENKRAQLGDLAQTTERQYGKC
                     LHQYTVKEAIHDKSVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLNSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLIQKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDKALSLYSNGGENDVLAPSWEEEKARFLEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAYQAFDKLYASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIAYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGMSTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            4527..>5474
                     /locus_tag="VH663_001506"
     CDS             4527..>5474
                     /locus_tag="VH663_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ8174691.1"
                     /translation="MTKSKQPQYRFDGFEGEWEEKRISDIANVFTGGGTPSTQNDQYW
                     TGNIPWIQSSDLSKDEILDVNPKKFITKLGLSNSAAKLIPKNSIAIVTRVGVGKLSII
                     NREYTTSQDFLSISDLKENLKFTVYSLYRLLQKELNQLQGTSIKGITKGYLLSKTILI
                     PSLPEQGAIGELFQMVDQLIQLQDQKLATLKEQKQTFLRKMFPAQGQKVPEIRLQGFK
                     GEWEEKKLGEVSTHRSGTAIEKYFDSEGEFKVISIGSYGTNNLYVDQNIRAVSNELTN
                     SKLVASGELTMVLNDKTANGAIIGRCLLITENNKYVVNQR"
BASE COUNT         1836 a         1082 c         1002 g         1554 t
ORIGIN      
        1 acaaaatgtt tctgtttttc atactatgct aatgagttag ctcgtgcgtc tccttcttca
       61 taggttactt ttaaccgcca aatgaatgct acacaatcga aaatagatga gtttacttat
      121 taaactgagc tatcaatagc ataaggttag ctgctaatat tttgatatta gaaagtgttg
      181 ttgttttatc tatcgctgct tctaaggaaa ttggctgctg ttgtattgaa aaaacagcca
      241 aaaagtcttc tgctgctagt cctgtctcaa tctttacaga accacataga gcaatcgttg
      301 gaacttgata ttttttagcc attcttgcaa tcgcaacagg taccttgcca gattgtgatt
      361 gtgtatccag gcatccttct ccagtaataa ctaaatcaca agactgaagg gaattatcca
      421 gatttagtag ctctgcaatc ctgctaaaac cactagttaa ggtgcctcct aataggacaa
      481 tggcacctcc taatcctcca gctgctccac ttcctggtat tgtctgtaaa tcaatggttg
      541 tttggcaaaa aactttttta gcaaagttac ttgcaatttg atcagtttct tcaatttgac
      601 taaggctccc acctttttga gggccaaaga ccgcagcaaa tccttgaggg ccatgatatg
      661 gattggtcac atcagtcagc cctaatagcg taacaggtga tgctaaagta tcaagataag
      721 atcttcctgt catgaaatca tagtttaaac tttccaaaaa ccctttgcca ccatcagaag
      781 tgcctgttcc tcctagcatg atttcgattt gagtagctcc tttttgaatg gcatctttaa
      841 ctgccaaacc caaaccatag gaagtggctt gtgcataggt tactgaattg ggggtgatct
      901 tatcaatacc aataatactc gcagattcaa taaaagcttg ctttgcgtga cgataataag
      961 cgactttaat cgggcgtctt aatagatcaa tggtttttac ttgatgccac ctccccgcca
     1021 cggtttgtga gagggctgct aaacttcctt ctcctccatc agcgatagct cttgtttcaa
     1081 taactaactg cttatcgaca gaaagaaggg cttgagctac actagtgtta agttctggag
     1141 acgttaccga tcccttaaaa gaatctatgg ctaccaaaat tttcatatct tcaataaatt
     1201 ccttttctgt atattgtagc agattatcgt cctttaaaac cctattaata gtcaagctag
     1261 atctgtctta agttttttat ctattttgag aactcgacct ttttactaat gttgtcttat
     1321 tactttcttc accttaaatt attgtcaagc aaactcaaaa atacaagaac tttctcgatt
     1381 gcatttacat agtcatagct ttgtttagtt gctaggatct taagcattca actaatcaaa
     1441 aaaagactgt ttggaaggtt tcaatagctt tgccattcta aaaaggatcc gccaatgata
     1501 taatattact atatcatgtc gaatggggat taaaaatggg aaatcaaact aaaacggaac
     1561 ttgaacttga aaaagagttg atccatctct tagaaacagg tgaaagtcaa tggacatatc
     1621 gtaaagaatt aaaaactgaa gatgctttat gggataattt ctttaagatc ttagcgcaaa
     1681 ataacacgca atatctgaac gaggagcctt taacagctag tgaaaaagag cagattaaga
     1741 atcaacttaa ctttgtcaat tactatgaag cggctaaatg gttagctggt gaaaacggca
     1801 ttgctaaagt acaagtgcaa cgcgaagatg caaaactagg aacgattcgt ttagaagtcg
     1861 ttaaagcaga caatgtggct ggtggaactt ctgtttatga gattgccaat caagttgctt
     1921 tctctggttc tcgtgataga cgaggtgatg tgacacttct catcaatggc ttacctatga
     1981 tccaaattga attaaaatct cagaatcatc aatgcattga agcttttaac caagtcaaaa
     2041 agtatgataa agaaggccag tttagaggta tcttttcaac cttacaaatg tttgtggtct
     2101 caaataaaac agatacaagg tatatcgctg cagctaaaga aaataaatta aaccctaact
     2161 tcttgaccca atgggtagat caaaacaaca aaccacaaaa agatctcttt gcctttgcca
     2221 aagaagtcct atctatccct cgtgctcacc aaatggtcat gacttactct gtcattgatg
     2281 atgataaaaa agcactaatt ctccttcgtc cttatcagat tcatgcaatc gaagccgtcg
     2341 ctgaagcgag tcgtcaccgc aagtctggct atatctggca cacaacaggt tctggaaaaa
     2401 ccttaacttc ctataaagtg gctcgcaata tccttcaaat accggccgtt gaaaaatcca
     2461 tctttgtcat tgaccgaaag gatttagata gtcagaccac ttctgctttt caatcttatg
     2521 cgcaaaatga tatctttgat gtcgatgaga cagaagatac gagacaactc atcaaaaatt
     2581 tagaatccag tgatcgtcgt gttgtggtca ccaccattca aaaactcaat gctatgatca
     2641 gtcagatgga aagttatgac actccaaaat tcaagaaact aaaagagcgt cttgaacacc
     2701 taaatgttgt ctttgttgta gacgaatgcc accgtgctgt cacgccagaa cgtcaacgtc
     2761 atctcacgaa tacttttcga aacagtcgct ggtatggctt tacaggtact cctatcttta
     2821 tggaaaataa gcgtgctcaa ttaggtgatt tagcccaaac aacggaacgg caatacggca
     2881 aatgtctcca tcaatacact gtcaaagaag ccattcacga taaatcggtt ctaggcttcc
     2941 aagttgagta taagacaacc attcctgata tgcctgaaga cagtattcct gaagaagctt
     3001 atgatcatga agaacatatg ttagctgtct taaatagtat cattaatcaa tcacgtaaga
     3061 agcttggctt taataatggg attggtcaga cattcgaagg cttattaacc gttaagagta
     3121 ttgctcgtgc ccaagcctat tatgatctga ttcagaaagt caaagctggt gaaacggatt
     3181 tagtcatttc taagaaggtc aaagaaaaac tgccagactt tccaaaggta gctattactt
     3241 attctatcac tgaaaatgac aatgcgtcta tcagccgaca agataaaatg accaaaaatc
     3301 tagaagacta taatcacctc tttggcacta actttaccat cgataactta caaggctata
     3361 acagagattt aaatgatcgc ttagcccgta aaaaagataa attcaaagac cgtcatgaac
     3421 aattagacct cgtgattgtg gttgaccgtc tcttaacggg ctttgatgcg ccttgtttat
     3481 caaccatctt catcgatcgc cagcctatga aacctcagca cattattcaa gctttctcac
     3541 gaaccaaccg tatctttgaa agtagaaaac attacggtca ggttgtgact ttccaaacac
     3601 cacttcgttt taaagaagct gttgacaaag cgcttagcct ctactctaat gggggtgaaa
     3661 acgacgtttt ggccccttct tgggaagaag aaaaagcaag attccttgaa aaagtaacag
     3721 tattaaaaaa tatagttcct gatccagacg ctttcccaac tattgagagc gcacagacag
     3781 cttttctcaa acaatacgcc aaagcctatc aagcatttga taagctctat gcatctgtac
     3841 aggtctatag tgactttaat gaaacactgt tgagtgaggt tgggttatca gatgaagtga
     3901 ttgatactta taaaggcact taccaaaatg tcattgcaga aatacgaaag cgaagagaag
     3961 atgacgaagc catcccagaa atcaatatcg actatgagct agaaagtgtt cagatggatg
     4021 atatcaacta tcactatatc ttgaccttaa ttcaagcctt tgtcgatcaa gaacaagaag
     4081 ctcttcaaga aaggctcaat gacaatccca tggatcaata catccaagat ttggcaaaat
     4141 caaaccctgc tatggctgac tcactagctg agttatggca agatatccaa aaagaaccca
     4201 aagcctatga agggaaaagc attgcttatg agttggataa cctcattggt gataaaatcc
     4261 aaagggccat caaacacttt gcggaccaat ggaaggcaga tcctgacaaa ttagcctttg
     4321 tggcgactaa ttatcatagt gctaactcta ccaaacaagt gggcatgtca accttaaaag
     4381 aatccctaga ctaccaagcc tataaagaaa aacaaggtga ttctgccatg aataaactca
     4441 aatacaagag ccaatttgaa cgggagttag ttcaattcat tagagatcaa atccaaccct
     4501 taaaagggca ctaggaaagg aaagagatga caaaatctaa acagcctcaa tatcgctttg
     4561 acggttttga aggggagtgg gaagagaaac gaatttctga tattgcgaat gtgtttactg
     4621 gaggtggcac acctagtact caaaacgatc aatactggac tggcaatatc ccctggatac
     4681 aatcatctga tttatccaaa gatgaaatat tagatgtaaa ccccaaaaaa tttattacaa
     4741 aattgggcct ttctaactct gctgcaaaat taataccaaa aaattctatt gctattgtta
     4801 cacgagttgg tgtaggaaaa ctttcaatta tcaataggga atacacaacc agccaagatt
     4861 ttttatctat ctcagatctt aaagagaact tgaaatttac tgtatattcg ctatatagac
     4921 tcttacaaaa ggaactaaat caattacaag gaacgtcaat taaaggtatc acaaaagggt
     4981 atctcctttc taaaacgata cttatcccct ctctccccga acaaggagcc attggcgaac
     5041 ttttccaaat ggttgatcaa ttgattcagt tgcaggatca aaagctagca acccttaaag
     5101 aacaaaagca aaccttcctc aggaaaatgt tcccagctca aggacaaaaa gtccctgaaa
     5161 tccgtttaca aggttttaaa ggagagtggg aagagaagaa attgggggaa gtatctactc
     5221 atagaagtgg aacggcaatt gaaaaatatt ttgattcgga aggtgaattt aaagtcattt
     5281 caataggtag ttatggaact aataacctct atgtagacca aaatattaga gcagtctcaa
     5341 atgaacttac taattctaaa ttagttgcct ccggtgaatt aacaatggta ctcaatgata
     5401 agacagcgaa tggggcaata atcggaagat gtctacttat tacagaaaat aataaatacg
     5461 ttgttaatca aaga
//
LOCUS       DAUGFP010000067         5449 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.067, whole genome shotgun sequence.
ACCESSION   DAUGFP010000067 DAUGFP010000000
VERSION     DAUGFP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.067"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..946)
                     /locus_tag="VH663_001507"
     CDS             complement(<1..946)
                     /locus_tag="VH663_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HEQ8174692.1"
                     /translation="MRKYDIQKLLLSGENQSVEFKEAKNSFPKDGMKTICSFANTNNG
                     LLILGVSENTKNKDFYISGVNDSDKVLDELYSLLNNPNKINRNVINEESVEVTNVKGK
                     EIIIIPVNKVDYKDKPIYLNDNIASTYFRQGTGDFRCSQEQINAMLRDSAKESFDSTL
                     VQNFSISDLDRETINRYREKFDDINAEHPFSKLDTEQFLLKINALRRDRTDNKIKPTV
                     AGLLIFGTHNAIKEFIPHYNVEYVLKDFTENNRFKDRVIYDGTWGEDNLFNFFHLVIE
                     KLYLTLNDNSNIQENSLNRIGISKLRIAIREAFVNSLIH"
     gene            1503..2135
                     /locus_tag="VH663_001508"
                     /pseudo
     CDS             1503..2135
                     /locus_tag="VH663_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411913.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2230..2481)
                     /locus_tag="VH663_001509"
     CDS             complement(2230..2481)
                     /locus_tag="VH663_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174693.1"
                     /translation="MPEKKVLPVLSMVVSFVCLLDHIFFFATYSYLYLPLVASSLALI
                     GLVQNKGRQKVWSLAGLVLAAIAVAVVAYAYYDPTVIPY"
     gene            complement(2715..3275)
                     /locus_tag="VH663_001510"
     CDS             complement(2715..3275)
                     /locus_tag="VH663_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174694.1"
                     /translation="MKTKSKRFLNLATLYLALLGTTLLMGRPVNADNADMDGYGSESK
                     VQREQSPYEKGVRDGHDAGYKQGKQDRQQGSPDASPTPPDKLRVPEPESNPYNKDHDK
                     QNNQRYKNGWDIAYRSSYYTGWYSAEGTGHLESDDYPNEEEGEDISDNSPSQGSTGST
                     DDGFSNLIDMVVDITTFLWGLVSNWF"
     gene            3975..4112
                     /locus_tag="VH663_001511"
     CDS             3975..4112
                     /locus_tag="VH663_001511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174695.1"
                     /translation="MLTYHELKKAVANRYIKGDMISITRKLVSSLILSFLIELYEYQE
                     F"
     gene            complement(4404..5057)
                     /locus_tag="VH663_001512"
     CDS             complement(4404..5057)
                     /locus_tag="VH663_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174696.1"
                     /translation="MKKTLTLLLALFAIGVTSSVRAEDAIKWELDPETSQNSPIAVNV
                     DNANKLRFTLPTGWSVSFKRKKDHYNPLEVNNKLVSETKNRNTSPRIVDYGKNGNPIL
                     AIYNDNQGSSGLPITVTVDKPNEGDELTLLYRADDGFFGGFTKLKDSKTLKKEKKEKE
                     RQLAEEKQIEEERKHVEQKQLEESMLKQIREEDHKPWHQRLSESIQDQWWNFKGLFQ"
BASE COUNT         1663 a         1060 c          946 g         1780 t
ORIGIN      
        1 tatgaataag gctgttaacg aatgcttcac gaattgcaat tcgtaacttt gaaatcccaa
       61 ttctattcag agaattttct tgaatattag agttatcatt taaagttaga tacaattttt
      121 caataactag gtgaaagaaa ttaaaaagat tatcttctcc ccacgtacca tcataaatga
      181 ctcgatcttt aaagcgatta ttttcggtaa aatcttttaa aacatattct acgttatagt
      241 gaggaataaa ctcttttatg gcattatgtg ttccaaaaat cagtaatcca gccacagttg
      301 gttttatttt gttgtctgta cgatctcttc ttagagcgtt tatttttaag agaaattgct
      361 cggtgtctaa tttggaaaaa gggtgttcag cattaatatc atcgaatttt tctctataac
      421 ggtttattgt ttctctatct agatctgata tagagaaatt ctgaacaagc gtactatcaa
      481 aactttcttt tgcagaatct cttagcatcg cattgatttg ttcttgtgaa cacctaaaat
      541 caccagtccc ctgacgaaaa taggtagatg ctatattgtc attaagatat attggtttat
      601 ccttgtaatc tactttgtta acagggataa tgataatctc ttttcctttt acatttgtga
      661 cctcaacact ttcctcgtta atcacatttc tattaatttt attagggtta ttaagtaaac
      721 tatataattc gtccaatact ttatcggaat cattaacgcc agaaatataa aaatctttat
      781 tcttggtgtt ttctgagaca cctagaatta acagaccatt atttgtattt gcgaaggaac
      841 aaattgtttt cataccgtct ttaggaaaag aatttttagc ttctttaaat tcaacacttt
      901 ggttctcccc tgataataac aatttttgta tatcgtattt tctcattttt tctccaatta
      961 attcgcacac gcattcgcac acacgtggga taataaattt gtttcctgtt cgaaaaaggc
     1021 gttattaaag gatttattga taacatcaat tcacacacgc attcgcacac gcgtgggaac
     1081 gtctaaaaat catctgagtt aatttattct tgttttactt ataacttctg ccagttattg
     1141 ttataccatt cccaaacggc atctcttggg aaagctaacc ttcttccata tcctctatca
     1201 atctgctgtg agatatctta acgttctaaa ctctcctttt tcttcatcaa gtataacatt
     1261 ttttagcgta ataaataaaa aaagtacgaa aaagcatcaa aaaagcctac cgactgcaat
     1321 cagagaggct ttaagttcaa aatccaccaa caggcaagat aggaactttg gtcagcctgt
     1381 ctgcccctac caaggatagt cgcgccattt cccaaccaca agacatgaac tccctatctt
     1441 tttagcgcaa gaattggtat aataaccctt gtcttaaaaa atgagcaaaa ggagagggaa
     1501 aaatgaaaaa gacattaacc ttgctactgg cactctttgc catcgggata actagtagcg
     1561 tcaaagcgga ggaggtattt gaatttaaac ctatcggtca acaaacagta ccaacagtta
     1621 cagtctcacc ttttaaaaca ggagagcaaa ctattgacat caccctccaa tcaaacggtt
     1681 acgtatatgc ttctataaaa cataaaaacg gagaaacaca aaaaattaaa acaaatctta
     1741 aataaatcac tgttacatgt ccagcagagc aaagcacctg tactgagcgt acttggatga
     1801 taagcggaaa tcgtgctgat aaatatggga attataccgt ccaactacaa aaaccacttg
     1861 cagttggaga cgtagttact ctatcctttg caaatgatgg tcatttttac ttgggtaaac
     1921 tagtcttcga agatgaaaaa acacaaaaca caaggttgca agcggaaaaa gcacaggaac
     1981 aagaagaggc aaaaaaacgc gataatgaat taagaaaaca actagaagaa agcatgctaa
     2041 agcagataag agaagaagac cataaatctt ggcatcagcg gttaagcgaa agcatccaag
     2101 atcagtggtg gaactttaag ggactgtttc agtgagggtt tacgaggcta tgaggttggc
     2161 taaagcacaa aaagcacctg tcaggggtgc tttttgtggt gctgattgcg tgcgaagggg
     2221 aggttaaggt tagtagggga tgacggttgg gtcatagtag gcataggcca ctactgccac
     2281 ggctatagca gctaagacga gcccagctag cgaccacacc ttttgcctgc ctttgttttg
     2341 cactaggccg ataagagcta gactacttgc tacaagcggc aagtagagat aactataagt
     2401 agcaaaaaag aagatatgat caagcaggca gacaaaactc accaccatgc ttaagacggg
     2461 caacactttc ttttctggca tacatgctcc tttctcaaca cacgggtcac tacaacctca
     2521 aaaaaggcca cccctatcag aggtggcctg actactaaag ggactatccc aattatagca
     2581 gattagtgtg ggaatggctt gagctaagga aagtatcctc aaattaagta tctcccctgt
     2641 tatttaaaaa taatttattt agtatattat ttctcggtaa atctaagcta cgaagctcaa
     2701 actaaagtat cattttaaaa ccaatttgac actagacccc aaagaaatgt ggtaatatca
     2761 acaaccatat caatgagatt agaaaaaccg tcatctgttg aacctgttga accctgtgaa
     2821 ggagagttgt cagaaatatc ttctccttcc tcttcatttg gataatcatc gctttctaaa
     2881 tgacctgtcc cttcagcaga ataccagcca gtataataag aagagcggta cgcaatatcc
     2941 cagccatttt tgtatctctg gttattctgc ttatcatgat ccttattata agggttactc
     3001 tcaggctcag gtactctaag tttatctgga ggggtaggag aagcatctgg ggatccttgt
     3061 tgtctatcct gtttgccttg cttataacct gcgtcatggc catctctcac acctttttca
     3121 tatggacttt gttctcgttg aacttttgac tcacttccat atccatccat atccgcatta
     3181 tccgcattta cgggacgtcc cattagcaaa gtcgttccta gtagggccaa gtaaagggtt
     3241 gctagattta aaaagcgttt tgattttgtt ttcataaagt cactccattt cattattttt
     3301 tgacacggtt atagtttact actcgcattc tttcatttca agttcatttt tttatcattt
     3361 taaatatcaa atgattagaa aaaaatggca agctatcctt atcacagaaa gtgcccgctt
     3421 ggagcagtta gataaccttt ttgagcttta ggaactgttt ttgaaagtat ttgatagttt
     3481 atataagagg gagggacttt gcatttgata aggaataacc gttgggcata ggccactact
     3541 gccacagcta gagctgctaa aacgagccca gctagcgacc aaaccttttg cctgcctttg
     3601 ttttgcacca gactaataat agctagacta cttgctacaa gcggcaagta aagataacta
     3661 taagtagcag aaagaagata tgatcaagca gggacacaag gctcactacc atgcttaata
     3721 cgagcagccc tttcttttct gacataaaaa tacctcttga gtagattttg gttatttagc
     3781 ctgtctactt aagacgtatc atatcatcaa tcgagggcga tttttttgtt tttgcacaca
     3841 caaacatcaa acatcttgat ttaaaatatt tacccgaaca tgtattcgca cacgggcata
     3901 tccgttataa tggacttttt ttaaatatct attttaagag attttttacg aatagattaa
     3961 gtaggaggat aaaaatgtta acatatcacg aattgaaaaa agctgtagct aacaggtata
     4021 tcaaaggaga catgatcagc attaccagaa aactggtaag ctctttgatt ttgtccttcc
     4081 tgattgagct ttatgaatat caagaatttt agtatcttta attaaaacat gattagggat
     4141 actgtccacg aacaaactaa tcagtcttgc ttcatgcgtt atcaggatat caaaagcaag
     4201 atttaattcc aaacttaatt gatttgtgtt atactcttta tacatcgtca tggctttcta
     4261 gttgttttgc cgttattata attataaacc atggcagaag aaaacgagca tctccgactg
     4321 cggaaatgct cgttttttat atgctaaagc tagtttttgc ctagcctctt gtgctttagc
     4381 caacctcata gcctagcaaa ccctcactga aacagtccct taaagttcca ccactgatct
     4441 tggatgcttt cgcttaaccg ctgatgccaa ggtttatggt cttcttctct tatctgcttt
     4501 agcatgcttt cttctagctg tttttgttca acgtgctttc tctcttcctc aatttgtttt
     4561 tcttctgcta gctgtctttc tttctccttc ttttcttttt ttagtgtctt cgaatctttt
     4621 aattttgtaa atccgccaaa aaaaccatca tcggcacgat aaagcagtgt taattcgtct
     4681 ccttcatttg gtttatcgac agttacagtg attggtaaac ctgatgaccc ttggttatca
     4741 ttataaatag ctaaaatagg attaccattt ttaccataat ctactatcct aggactagta
     4801 tttctgtttt ttgtctcaga tactagctta ttatttacct ctagaggatt gtaatgatct
     4861 ttttttcttt taaaagatac cgaccagcct gtaggtagtg tgaaccgtag tttattagcg
     4921 ttatcaacgt tcacagcaat tggactattt tgagaagttt ctggatctag ttcccacttg
     4981 attgcatcct ccgctctgac gctactagtt accccgatgg caaagagtgc cagtagcaag
     5041 gttaatgtct ttttcatttc attctctcct tttgctcatt ttttaagaca agggttatta
     5101 taccaattct tgcgctaaaa agatagggat tgattggctg aggcgaccgg agaagtcctt
     5161 gatctctctg agtagctgct gctcacttaa tgtttcacta ttttattggg atatgctaat
     5221 acttcaagat atctttatat cgtctatagt caagagccta tttgacactt tatctctatt
     5281 aaataatttg cacactctgt taagtgctct ttttggtcgc ttattctctc ttaaagttgt
     5341 tttatgtcag ttgaaaacga cgtttcttga gtaacctaac ttacctgata tctcgtatcg
     5401 tgatatgtta tttatcaata ttatgaaata gaacaacgat attaggcat
//
LOCUS       DAUGFP010000068         5154 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.068, whole genome shotgun sequence.
ACCESSION   DAUGFP010000068 DAUGFP010000000
VERSION     DAUGFP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.068"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            101..1649
                     /locus_tag="VH663_001513"
     rRNA            101..1649
                     /locus_tag="VH663_001513"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1701..1773
                     /locus_tag="VH663_001514"
     tRNA            1701..1773
                     /locus_tag="VH663_001514"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1734..1736,aa:Ala,seq:tgc)
     gene            2072..4974
                     /locus_tag="VH663_001515"
     rRNA            2072..4974
                     /locus_tag="VH663_001515"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5060..>5154
                     /gene="rrf"
                     /locus_tag="VH663_001516"
     rRNA            5060..>5154
                     /gene="rrf"
                     /locus_tag="VH663_001516"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1462 a         1062 c         1480 g         1150 t
ORIGIN      
        1 agacgaacca aacgtgaggg tgatatggag acatattacc cgtcaagaaa cgagaaataa
       61 atctgtcagc gacagaagga acgagtaagt tcaaacgcaa attaaagaga gtttgatcct
      121 ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct gagaactggt
      181 gcttgcaccg gttcaaggag ttgcgaacgg gtgagtaacg cgtaggtaac ctacctcata
      241 gcgggggata actattggaa acgatagcta ataccgcata agagagacta acgcatgtta
      301 gtaatttaaa aggggcaatt gctccactat gagatggacc tgcgttgtat tagctagttg
      361 gtgaggtaaa ggctcaccaa ggcgacgata catagccgac ctgagagggt gatcggccac
      421 actgggactg agacacggcc cagactccta cgggaggcag cagtagggaa tcttcggcaa
      481 tgggggcaac cctgaccgag caacgccgcg tgagtgaaga aggttttcgg atcgtaaagc
      541 tctgttgtta gagaagaatg atggtgggag tggaaaatcc accaagtgac ggtaactaac
      601 cagaaaggga cggctaacta cgtgccagca gccgcggtaa tacgtaggtc ccgagcgttg
      661 tccggattta ttgggcgtaa agcgagcgca ggcggttttt taagtctgaa gttaaaggca
      721 ttggctcaac caatgtacgc tttggaaact ggagaacttg agtgcagaag gggagagtgg
      781 aattccatgt gtagcggtga aatgcgtaga tatatggagg aacaccggtg gcgaaagcgg
      841 ctctctggtc tgtaactgac gctgaggctc gaaagcgtgg ggagcaaaca ggattagata
      901 ccctggtagt ccacgccgta aacgatgagt gctaggtgtt aggccctttc cggggcttag
      961 tgccggagct aacgcattaa gcactccgcc tggggagtac gaccgcaagg ttgaaactca
     1021 aaggaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg
     1081 aagaacctta ccaggtcttg acatcccgat gcccgctcta gagatagagt tttacttcgg
     1141 tacatcggtg acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa
     1201 gtcccgcaac gagcgcaacc cctattgtta gttgccatca ttaagttggg cactctagcg
     1261 agactgccgg taataaaccg gaggaaggtg gggatgacgt caaatcatca tgccccttat
     1321 gacctgggct acacacgtgc tacaatggtt ggtacaacga gtcgcaagcc ggtgacggca
     1381 agctaatctc ttaaagccaa tctcagttcg gattgtaggc tgcaactcgc ctacatgaag
     1441 tcggaatcgc tagtaatcgc ggatcagcac gccgcggtga atacgttccc gggccttgta
     1501 cacaccgccc gtcacaccac gagagtttgt aacacccgaa gtcggtgagg taacctatta
     1561 ggagccagcc gcctaaggtg ggatagatga ttggggtgaa gtcgtaacaa ggtagccgta
     1621 tcggaaggtg cggctggatc acctcctttc taaggaaatg gaacacgttt atcgtcttat
     1681 ttagttttga gaggtcttgt ggggccttag ctcagctggg agagcgcctg ctttgcacgc
     1741 aggaggtcag cggttcgatc ccgctaggct ccatcaggat acaatcctac taaacttaat
     1801 acaagtgaag ttgaacacgc aactcacttc ctaggaaaat agacaatctt cccttgtgtg
     1861 caaggcccac atggtcagat tcctaatttt ctacagaagt ttcgctaaag cgagcgttgc
     1921 ttggtatcct atataatagt ccattgaaaa ttgaatatct atatcaaatt ccacgatcta
     1981 gaaatagatt gtagaaaagt aacaagaaaa taaaccgaaa acgctgtgac tatttaataa
     2041 gttttctagt ttaaaaaaac taggttaata aggttaagtt aataagggcg cacggtggat
     2101 gccttggcac tagaagccga agaaggacgt gacaaacgac gaaatgcttt ggggagctgt
     2161 aagtaagcgc tgatccagag atgtccgaat gggggaaccc actaactaat ggttagtatc
     2221 cataactgtt aaggttatga gaaggaagac gcagtgaact gaaacatcta agtagctgca
     2281 ggaagagaaa gcaaacgcga ttgccttagt agcggcgagc gaaacggcag gagggcaaac
     2341 cgaagtgttt acacttcggg gttgtaggac tgcgacgtgg gactttaaaa ggatagaaga
     2401 attacctggg aaggtaagcc aaagagagta aaagcctcgt atttaaaatt cttttgagcc
     2461 ctagcagtat cctgagtacg gcgagacacg tgaaatctcg ttggaatctg ggaggaccat
     2521 ctcccaaccc taaatactct ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg
     2581 aaaagcaccc cgggagggga gtgaaataga acctgaaacc gtgtgcctac aacaagttcg
     2641 agcccgttaa tgggtgagag cgtgcctttt gtagaatgaa ccggcgagtt acgatatgat
     2701 gcgaggttaa gttgaagaga cggagccgta gggaaaccga gtcttaatag ggcgatttag
     2761 tatcatgttg tagacccgaa accatgtgac ctacccatga gcaggttgaa ggtgaggtaa
     2821 aactcactgg aggaccgaac cagggcacgt tgaaaagtgc ttggatgact tgtgggtagc
     2881 ggagaaattc caaacgaact tggagatagc tggttctctc cgaaatagct ttagggctag
     2941 cgtcgatgtt aagtctcttg gaggtagagc actgtttggg tgaggggtcc atctcggatt
     3001 accaatctca gataaactcc gaatgccaac gagatatcat cggcagtcag actgcgagtg
     3061 ctaagatccg tagtcgaaag ggaaacagcc cagaccacca gctaaggtcc caaaataact
     3121 gttaagtgga aaaggatgtg gggttgcaca gacaactagg atgttagctt agaagcagct
     3181 attcattcaa agagtgcgta atagctcact agtcgagtga ccctgcgccg aaaatgtacc
     3241 ggggctaaaa cagtttaccg aagctgtgga tgacacaaaa gtgtcatggt aggagagcgt
     3301 tctatgtgtg aagaaggtgt accgtgagga gcgctggaac gcatagaagt gagaatgccg
     3361 gtatgagtag cgaaagacag gtgagaatcc tgtccaccgt aagactaagg tttccagggg
     3421 aaggctcgtc cgccctgggt tagtcgggac ctaaggagag accgaaaggt gtatccgatg
     3481 gccaacaggt tgatattcct gtactagagt atatagtgat ggagggacgc agtaggctaa
     3541 ctaaaccgga cgattggaag agtccggcta agcagtgagg tgtaagatga gtcaaatgct
     3601 tatctttata acattgagct gtgatgggga gcgaatttta gtagcgaagt tagtgatgtc
     3661 acactgccaa gaaaagcttc tagcgtttaa tgatactcta cccgtaccgc aaaccgacac
     3721 aggtagtcga ggcgagtagc ctcaggtgat cgagagaact ctcgttaagg aactcggcaa
     3781 aatgaccccg taacttcggg agaaggggtg ctgacttagg tcagccgcag tgaataggcc
     3841 caagcaactg tttatcaaaa acacagctct ctgctaaatc gtaagatgat gtataggggg
     3901 tgacgcctgc ccggtgctgg aaggttaaga ggagggttta gcgcaagcga agatctgaat
     3961 tgaagcccca gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
     4021 cgggtaagtt ccgacccgca cgaaaggcgt aatgatttgg gcactgtctc aacgagagac
     4081 tcggtgaaat tttagtacct gtgaagatgc aggttacccg cgacaggacg gaaagacccc
     4141 atggagcttt actgcagttt gatattgagt atctgtacca catgtacagg ataggtagga
     4201 gccattgact tcgggacgcc agtttcgaat gaggcgttgt tgggatacta cccttgtgtt
     4261 atggctactc taacccagat aggttatccc tatcggagac agtgtctgac gggcagtttg
     4321 actggggcgg tcgcctccta aagagtaacg gaggcgccca aaggttccct cagattggtt
     4381 ggaaatcaat cgcagagtgt aaaggtataa gggagcttga ctgcgagagc tacaactcga
     4441 gcagggacga aagtcgggct tagtgatccg gtggtaccga atggaagggc catcgctcaa
     4501 cggataaaag ctaccctggg gataacaggc ttatctcccc caagagttca catcgacggg
     4561 gaggtttggc acctcgatgt cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt
     4621 tgggctgttc gcccattaaa gcggcacgcg agctgggttc agaacgtcgt gagacagttc
     4681 ggtccctatc cgtcgcgggc gtaggaaatt tgagaggatc tgctcctagt acgagaggac
     4741 cagagtggac ttaccgctgg tgtaccagtt gtcttgccaa aggcatcgct gggtagctat
     4801 gtagggaagg gataagcgct gaaagcatct aagtgcgaag cccccctcaa gatgagattt
     4861 cccatgattt tatatcagta agagccctga gagatgatca ggtagatagg ttaggagtgt
     4921 aagtgtagcg atacatgtag cggactaata ctaatagctc gaggacttat ccaaaaagaa
     4981 atattgacaa cgttacggat tcttgttaga ctatagatat tcaattttga gtgggttatg
     5041 aaaatagcac atttacaagt taagtgacga tagcctagga gatacacctg tacccatgcc
     5101 gaacacagca gttaagccct agaacgcctg atgtagttgg gggttgcccc ctgt
//
LOCUS       DAUGFP010000069         4911 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.069, whole genome shotgun sequence.
ACCESSION   DAUGFP010000069 DAUGFP010000000
VERSION     DAUGFP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.069"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..677
                     /gene="tilS"
                     /locus_tag="VH663_001517"
     CDS             <1..677
                     /gene="tilS"
                     /locus_tag="VH663_001517"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEQ8174697.1"
                     /translation="IQGLNQLALENSLLFQAFKELTNHITTTDLTEFNEQSKSIQYFL
                     LQDYLEGFPDLDLKKSQFTQLLQIIQTAKQGYYYLKKDYYIFIDKFSFKITKIVPKTE
                     LVKDEKMLEYDSNLCYRDYCFSFMPKSNEDQGQVSIPLFSLSSIKLRSRQSGDYISFG
                     HFSKKIRRLFIDEKFTIAERQNAIIGEQDEQIIFVLIGNKTYLRKACKHDIMLAKLYI
                     DKLEKG"
     gene            682..1224
                     /gene="hpt"
                     /locus_tag="VH663_001518"
     CDS             682..1224
                     /gene="hpt"
                     /locus_tag="VH663_001518"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEQ8174698.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
     gene            1246..3225
                     /gene="ftsH"
                     /locus_tag="VH663_001519"
     CDS             1246..3225
                     /gene="ftsH"
                     /locus_tag="VH663_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEQ8174699.1"
                     /translation="MKNNKNNGFVKNSFIYILMIIVVITGFQFYLKGTSTQSQQISYS
                     KLIKHLKAGDIKSLSYQPSGSIIEVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSL
                     VLPSDTILKEMTAAADKNGTELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGG
                     GGARGAMSFGKNKAKSQSKGDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFV
                     GADLENVLNEAALVAARRNKIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGR
                     VAEEIVFNAQTSGASNDFEQATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEK
                     AYSAQTAQMIDDEVRELLNQARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIY
                     ETGKMPVDLETDDNEAHALSYDEIKNKMTESESAE"
     gene            3551..>4911
                     /locus_tag="VH663_001520"
     CDS             3551..>4911
                     /locus_tag="VH663_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ8174700.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLIFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLA"
BASE COUNT         1652 a          711 c          929 g         1619 t
ORIGIN      
        1 tcattcaggg attaaatcaa ttagcacttg aaaatagctt actttttcaa gcttttaagg
       61 agctaacaaa tcatattact accactgatt taacagaatt taatgagcaa tctaagtcta
      121 ttcaatactt tttgttacaa gattatttag aaggttttcc agatttagat ttaaaaaaat
      181 ctcaatttac tcaattattg caaattatcc aaactgctaa acaaggctat tattatctca
      241 aaaaagacta ttatatattc attgataaat tctcttttaa aattacaaaa atcgttccta
      301 agactgagtt agttaaagat gaaaaaatgt tagaatacga tagtaactta tgctacagag
      361 attattgttt ttcatttatg ccaaaaagta acgaagatca aggacaggta agtatacctt
      421 tatttagttt atcgtctatt aaattaagaa gtcgccaatc tggagattat atttcttttg
      481 gtcacttttc aaaaaaaata agacgtttat ttatagatga aaaattcaca atagctgaac
      541 gccaaaatgc tattattgga gagcaagatg agcagattat atttgtattg ataggtaata
      601 aaacttattt gagaaaagct tgtaaacatg atataatgtt ggctaaactg tatattgata
      661 aattagaaaa agggtgacag catgctcgag caagatattc aaaaaattct ttactctgaa
      721 aatgatatta ttcggaaaac taaaaaatta ggagaacaat taacaaaaga ttatcaagaa
      781 aaaaatccgc ttatgattgg tgtattaaaa ggatcagttc cttttatggc agaactaatg
      841 aaacacatcg atactcatgt tgaaattgat tttatggtag tatcaagtta tcatggcggt
      901 acatctagca gtggtgaagt taaaatttta aaagatgtag ataccaatat tgaaggaaga
      961 gatatcataa ttgttgaaga tattattgac accggtcgca ccttaaaata tttacgagat
     1021 atgtttaagt accgtaaagc taatactatc aaaattgcaa cactgtttga taaacctgaa
     1081 ggtcgtgttg ttaaaattga agcagactat gtttgttaca atattccaaa tgaatttatc
     1141 gtaggctttg gtttagatta cgcagaaaat tatcgcaatc ttccttacgt aggtgtatta
     1201 aaagaagaag tctattcaaa atagaaaaag gtcaatgttt actgtatgaa aaataataaa
     1261 aataatggtt ttgttaaaaa ttcttttata tatatattga tgattattgt tgtcatcaca
     1321 ggatttcaat tttatcttaa gggaaccagc acacaaagcc aacagattag ttattccaaa
     1381 ttaattaaac atcttaaggc tggagatata aaatctttaa gttatcagcc tagcggtagt
     1441 attattgaag ttaaagggaa atatgagaaa cctcaaaaag tgacagttaa ttcaggacta
     1501 tcatttttag gtggaagagc ttcaacacag gtaacagagt tctcatcttt agtattacct
     1561 agtgatacca tcttgaaaga aatgacagca gcagctgata aaaatggaac agagcttaca
     1621 gttaagcagg aaagttcaag tggtacttgg attaccttct taatgagctt tttacctatt
     1681 gttatttttg ctgctttcat gatgatgatg atgaatcaag gtggtggagg agctcgtggt
     1741 gctatgagtt tcggcaaaaa taaggcaaaa tctcaatcaa aaggtgatgt taaagtgaga
     1801 tttacagacg ttgctggtgc cgaagaagaa aaacaagaat tagtagaagt tgttgatttt
     1861 ttgaaaaatc ctaaaaaata taaatcacta ggagcacgga ttccagcggg agttcttcta
     1921 gaggggcctc caggtactgg taaaacatta ctagctaaag ctgtagcagg tgaagctgga
     1981 gtgccatttt tcagtatttc aggttcagat tttgtcgaaa tgtttgtcgg tgtaggtgct
     2041 agtcgtgttc gttctttatt tgaggatgct aaaaaagctg agcgtgcgat catctttatc
     2101 gatgaaattg acgctgtagg ccgtcgtcgt ggtgctggca tgggaggcgg taacgatgaa
     2161 cgagaacaaa cccttaacca attactcatt gaaatggatg gttttgaggg taatgagaac
     2221 attatcgtta ttgctgcaac taaccgtagt gatgttttag atccagcttt actaagacca
     2281 ggtcgttttg atcgtaaggt tttagttggt cgccctgatg ttaaaggacg tgaagccatt
     2341 ttacgtgttc atgctaaaaa taaaccactt gcaaatgacg ttaatttgaa agtagtagcc
     2401 caacagactc caggttttgt aggtgctgat cttgaaaatg ttttaaatga agctgcgtta
     2461 gttgctgctc gcagaaataa aattaaaatt gatgctagtg atattgatga agctgaagat
     2521 cgtgttattg ctggaccatc aaagaaagat cgcactattt cgcaaaaaga gcgagaaatg
     2581 gttgcttatc acgaagctgg tcatactatt gttggtttag ttttatctaa tgcgcgtgta
     2641 gttcacaagg taactattgt acctcgtgga cgagcaggtg gttatatgat cgctcttcca
     2701 aaagaagatc aaatgcttct ttctaaagaa gatctgaaag agcaattagc aggattgatg
     2761 ggtggtcgtg ttgctgaaga aattgttttt aatgctcaaa catcaggtgc gtcaaatgac
     2821 tttgagcaag ctactcagat agcaagggcc atggttacgg aatatggtat gagtgaaaag
     2881 cttggaccag ttcaatatga aggtaaccat gcgatgatgc caggacagat ctctcctgaa
     2941 aaagcttact ctgcccaaac agctcaaatg attgatgatg aggttcgtga actcttaaat
     3001 caagctcgta atcaagcagc tgatatcatc aatgagaatc gagatactca taaattgatt
     3061 gctgaagctc ttctcaaata tgaaactcta gacgctgcac aaattaaatc aatttatgaa
     3121 actggtaaaa tgccagttga tttagaaact gatgataatg aagctcatgc gttatcttat
     3181 gacgaaatta aaaataaaat gacagaatct gaatctgcag aatgagataa aaaagctgaa
     3241 cttatagatt aagttcagct tttttatcaa cacaatatcc ttgtaaacgt tatgaattgt
     3301 aataggtgcc taattgctag aaatgagaag atgacatact ttttttcttt attacttaaa
     3361 gtgagaaaga cttttgtatt taaaactaga aaattgttaa agccatttga aaaaatctat
     3421 tagtgttttc ttagaataat agaagaacag aagatagcaa aagtttcagt caaccaataa
     3481 gatgaaattt acaatcatta ctaactatga tacacttagt agaaaatcat ttactataga
     3541 aggtgataac atgactattt ttcgtaaaaa aaagaaatat tccaataaaa cagaaatgca
     3601 aaggcacttc aaagttattg atttaatttt tttaggccta ggttcaatgg ttggaacagg
     3661 tatctttaca gttactggca taggcgctgc taagtatgca ggtcctgctt taacaatatc
     3721 gattatcatt tccgcgattg ctattggaat tttagcctta ttttatgcag aatttgcgtc
     3781 tcgtatgcct tctaacggtg gtgcttatag ttatgtttat gcaaccttgg gagagtttcc
     3841 agcatggctt gttggctggt atattattat ggaattttta acagctatat ctagtgttgc
     3901 tgttggctgg ggaagttatt taaaaggatt acttgccaat tataatatag tattaccaaa
     3961 cgctttaaat ggcacgttca atcttaagaa tggaacttac atagatattc ttcctgtttt
     4021 agtgatgttt ttcgtgacag ggattgtcct aatgaattcg aaattagccc tgagattcaa
     4081 tagtttttta gttattttaa aattttcagc actggcttta tttatctttg taggcatttt
     4141 ttttattgat cacaataatt ggtctcattt tgctccatat ggtgtaggac aaattactgg
     4201 aggtaaaaca ggtatatttg ctggtgcttc tgttatgttc tttgcatttt taggatttga
     4261 atctatttca atggcagttg atgaagtgaa agaaccacaa aaaacaatcc ctaaaggcat
     4321 cattttatct ttgattattg taacagcact ctatatagtt gtcaccacta ttttaacggg
     4381 aattgtacac tacactaaat taaatgttcc agacgcggtg gcttttgctt taagaaatat
     4441 tagactttat tgggcagctg actatgtgtc gatagttgct attttgactc ttattacggt
     4501 atgtatttct atgacctatg cattagctag aacaatttac agtattagta gagatggttt
     4561 attacctaaa tcactttata ctctcactaa aaaaaataaa gtgcctcaaa atgctactct
     4621 tgtcactgga ctacttgcta tgatttgtgc gggaatattt cctttatcga gtctagcaga
     4681 gtttgttaat atttgtactt tagcatattt aattattctg tcgggtgcaa ttattaaact
     4741 cagaagaata gaaggagaac caaaagcaaa tgaatttaaa actcctctag taccattcct
     4801 accaatgttg gcaattatca tttgtctatc atttatgagc caatataaag cttttacttg
     4861 gattgctttt gctattgcca caattattgg gacattaatc tacttagctt a
//
LOCUS       DAUGFP010000070         4879 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.070, whole genome shotgun sequence.
ACCESSION   DAUGFP010000070 DAUGFP010000000
VERSION     DAUGFP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.070"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(59..745)
                     /locus_tag="VH663_001521"
     CDS             complement(59..745)
                     /locus_tag="VH663_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8174701.1"
                     /translation="MYKILAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFK
                     GYDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKP
                     FSLKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYR
                     EYEILELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKT
                     IRGIGYKWDV"
     gene            complement(896..1591)
                     /locus_tag="VH663_001522"
     CDS             complement(896..1591)
                     /locus_tag="VH663_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
                     /protein_id="HEQ8174702.1"
                     /translation="MEHQIERLIAEDIYIKVSFGVKQSGLIFIPNYQLDIRKEENHKK
                     YKVYIRETAQFFIYNKEASELNRYMRGHELICQLTNDSKSIPKRRRQTIDTLKKKIEE
                     ISLLIELDTENKPYQDIKDDIVKDMAQLDLTITELQDHIAHLNKVAEVLLNLNNNDIE
                     NRRLARYDYAKMNLTAAIKIEEVEKEIETSQNELNISIDEYEYLVRRLEKFGEILSDS
                     KIIDTSRNEIQWE"
     gene            complement(1857..2066)
                     /locus_tag="VH663_001523"
     CDS             complement(1857..2066)
                     /locus_tag="VH663_001523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174703.1"
                     /translation="MTDRVMKKPIRGRQLSKRDLYDEEFFRTHFAKIEIESRLEFLLN
                     RANSLEELLLKAKEVNLTIDLKQKM"
     gene            complement(2112..2389)
                     /locus_tag="VH663_001524"
                     /pseudo
     CDS             complement(2112..2389)
                     /locus_tag="VH663_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883480.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2380..2705)
                     /locus_tag="VH663_001525"
                     /pseudo
     CDS             complement(2380..2705)
                     /locus_tag="VH663_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(2885..3484)
                     /locus_tag="VH663_001526"
                     /pseudo
     CDS             complement(2885..3484)
                     /locus_tag="VH663_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            complement(3783..4148)
                     /gene="rplL"
                     /locus_tag="VH663_001527"
     CDS             complement(3783..4148)
                     /gene="rplL"
                     /locus_tag="VH663_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEQ8174704.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
     gene            complement(4213..4713)
                     /locus_tag="VH663_001528"
     CDS             complement(4213..4713)
                     /locus_tag="VH663_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEQ8174705.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     misc_feature    complement(4743..4878)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT         1441 a         1023 c          732 g         1683 t
ORIGIN      
        1 aactccaaag acaatttcaa cgacggcgcg ccaaacacga ttttttattg aatatttttt
       61 aaacatccca tttataacca atcccgcgaa tagtttttat tggattaatt ccaaaacttg
      121 aaaatttcat tctaatctga tagatatatt ctgaaataga atgaaacaat gctgaagctt
      181 catcgctata tacttgttca tatatctctt cttttgtgaa aactttatac ggatgacgtg
      241 acagtaattc taaaatttcg tactctctat aagtcaggaa taacggctgt ttatcaatat
      301 ataacatacg ttcctctaga tagatttcta taggtggaaa acttcgcttc actctctcct
      361 cagcctcatt ctttatcttt gctctctctt ctctcttcaa atgagagtta actttagcta
      421 cgaattgttt taagctaaat ggtttcacaa taaaatcgtc tccccctata tttaaagctt
      481 ggacaatttc attatcgcca tctaaagcac taacaaaaat aattggagaa tgaacttctt
      541 cccgaatttt ataacaaaac tcagttccac ttatattagg catcatgata tccaataaga
      601 tcaaatcata tcctttaaaa tcatcaaaaa ctattggtaa ttctatctct tgacaggtta
      661 taacatggta attttcaatc tccaaagcag tcttcataag cttcagaatt tctttatcat
      721 catctatggc taaaatctta tacattatta ccccttttaa ataatgaaat acttacaaat
      781 aaactattct caatgtaagc ggcaggaatc atttctttaa tatagttatt tacgaaacat
      841 caactagatt ttaatatctg atataaaaat tgctaccacc caaattatat cataattact
      901 cccattgtat ttcatttcga gaagtatcga taattttgct atcactcaag atctctccaa
      961 acttttccaa ccttcttact agatattcat actcatctat ggatatatta agttcatttt
     1021 gagaagtttc aatctctttc tcaacttctt ctatttttat agctgcagtc aagttcatct
     1081 tggcatagtc atatctggcc aaacggcggt tctctatatc gttgttgttc agattaagca
     1141 agacctccgc aaccttattg agatgagcaa tatggtcctg caactctgta atggtcaaat
     1201 ctaattgagc catatcttta acaatgtcat ctttaatatc ttgataaggc ttattctccg
     1261 tatctaactc aatgaggaga cttatttctt caatcttttt cttaagtgta tcaatggttt
     1321 gtcgtcttct ttttggaata cttttgctat cattggttaa ctggcaaatg agctcatgcc
     1381 ctcgcatata gcgattaagt tcagacgcct ccttgttgta aatgaagaat tgtgcggtct
     1441 cacgaatata gactttatat tttttatgat tttcttcttt cctaatatcc aattgatagt
     1501 taggaatgaa aataagacca ctctgcttta caccaaaaga caccttgata taaatgtcct
     1561 cagcaattaa cctctcaatt tgatgttcca caagttcaac ttcaaactca tggatggtat
     1621 ctcgttgccc cttgtatttc ttgaagactt ccttactctc ttcagtggat accttctctt
     1681 tatctcgttc ttcttgaaaa gcatgtattg tttctgtaga ctttctaatc gatctgaaac
     1741 accgacttcc ttatttttta aagtaatctt gaaaaaattg acatcatata atttcttatc
     1801 gcttattttt ttatgaccta aacttatctt ttgagtatct tcttctagga taaaagttac
     1861 attttttgtt ttaagtcaat ggttagattc acttcttttg cttttaatag tagctcctct
     1921 aatgaattgg cacggttcaa caaaaattct aatcgacttt caatttctat cttggcaaaa
     1981 tgtgtacgaa aaaattcttc atcatataaa tctcgtttac tgagttgacg gcctcgaatt
     2041 ggttttttca ttactcgatc cgtcatcatg aatctgctat gcttctgact aaaatcaata
     2101 tgaacatgta attactctgc tttttctaaa aaatcatcaa agaactttga ctgctgcatc
     2161 aaaaaataaa gacgttgctt tagttcaaac ttatggctag tcgctttata tttttgatag
     2221 tcacggtaag agaaatgctt ttcaataatt ttcgccccag ctattttaga aatacggtct
     2281 gagatcatac gaagatttcg ttccaaggca taattccata tcaatttttt atctgaatta
     2341 cggtcaatgg cgctgattgg atgtgattat gggtatgatt tttatctgtt tggtcgctcg
     2401 agtaatctca aagaagagag ttctcctagt gccctcaaat cccagtcgtc tgcctgcgag
     2461 tgaaaatgct tgacaaggga aaaaccaccg cagatgatat ccacttgccc tctaagttgt
     2521 ctaaagtctt ggtctgtgac ttgtctaatg tcatgaaatt ctatttctcc ttcggtctca
     2581 tagatggctt tgtaagattg cctcgcaaac ttatcaatct cacaaaagcc aatacactca
     2641 tggcactgat taattaaacc caatcgaaag cctccaattc ctgcaaacaa atctaaaaat
     2701 ttcatatctc tcattccttt ctgacaaaat aaaaggacct tactgcttat gtaacgtcct
     2761 tactcaacta taatcttcca tttctggtgc agtgataact tcaataatca accaaattgt
     2821 ccttagtccc caaacaccta ataatagcca ccaaaacatc ttactcacct cacttcgctt
     2881 cttgtcacct tagttgcttg gtccattcgg ataagacacc attaaagaca tattccgcaa
     2941 tagcttcagc tgttcttgcg aaccgtatgt gttccagcgc ataagcatag cgttcttcaa
     3001 aatagctcat gactaagtca ctagcagctt gtgataaacc accttcacgt aattgggaat
     3061 gaaccaaact ccaaatataa tctttgtcat atttggtgat ctgatcaact tgacgggtaa
     3121 agtgaccctc agtctttttc acactagtgc actcctttct ttcctcctcg tcctcaatga
     3181 ctaaattact ctctttagtt tcactacctt cagtttcact cttttcagtc tcactagggg
     3241 ctgaatttaa cacggggctg gtctgttttt gagactggcc ccgtctgaac ttaagactgg
     3301 ggtggaatca tgttccaact ttcctaaata aatcttatta gccaaacgcc ctttttcact
     3361 gggagactgt tggacttcat cgatcaaacc atagtctcgt aggttttctt aatcgctaac
     3421 aatttcgact tggaacagcc aaggagtact atcattttga gttggaataa atgagataga
     3481 tggcaatcac catttgtcgt gatgactgtt cattcatttg catgattttc ctcctgaaaa
     3541 taaaaaaatg agaacactta ttaaaatgtt ctcgttcaca taaaaaaatt attactagac
     3601 acatatagat agaataacca ctctactcag cttaattttt atagaaaata aaaatcctgc
     3661 caaagatttt ttgggcagga tttttggcaa gaaaccaaat caatttatca gtttcttcaa
     3721 atcgctataa actctaaaag actgataaat cgggatttcg ctctcaaatt gctatctgct
     3781 tcttatttaa gagtgattgt tgctccagct tcttcaagtt tagctttgat ttcttcagct
     3841 tctgcagcag caacgccttc tttaacgtta gcaggtgctc catcaacaag acctttagct
     3901 tctttaagac caagacctgt gatttcacga acagctttga taacgccaac ttttttgtcg
     3961 ccagcagatg tcaattcaac gtcgaatgaa tctttagcag cttcttcagc accaccagca
     4021 gctgcagcag ctacaggagc agctgcagtt acaccaaatt cttcttcgat agcttttaca
     4081 agatcgttaa gctcaaggat tgaagcttct ttaatttcag caataatgtt ttcaatgttc
     4141 aatgccattg tgatttcctc caaataatta atttaaaatg ttaagcgtag ccttaagcta
     4201 ctctgagacg acttaagcag cgccttcttt gttttctgca acagctttga cagcgtatgc
     4261 aacgttgcgg actggagctt gaagtacaga aagaagcata gaaagcattc cttcgcggtt
     4321 tggcaatgtt gcaagtgctt ggatttcttc ttttgaagaa acagcgcctt cgattgcacc
     4381 acctttgatt tcaagtgcgt cagcagtttt agtaaagtcg ttgataactt tagctggtgc
     4441 gataacatct tcgttagaaa atgctactgc agatggtcct acgaatacat ctttcaactc
     4501 atcaagacct gctttttcag ctgcacgagt caaaattgag tttttgataa ctttgaactc
     4561 aacgccactt tcacgaagtg aacgacgaag aacagtatct tgatcaactg taagaccacg
     4621 tgaatcaaca acaacgatac ttgctgctgc tttcattttc tcagcaataa gttcgacttg
     4681 ttcagctttt ttagcaataa ttgcttcact cattagtttt acctccgttt ttatttttgg
     4741 gctaggtaca aaaaaaatcg cacctaaacc tagacacgaa agtacaaata cgttattttc
     4801 tttaatcacg ttttgtgcct cggcaggaat attatgagca tgctcccctg ctgtcttagg
     4861 tcagtttttt atcaaaacc
//
LOCUS       DAUGFP010000071         4772 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.071, whole genome shotgun sequence.
ACCESSION   DAUGFP010000071 DAUGFP010000000
VERSION     DAUGFP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.071"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(9..251)
                     /locus_tag="VH663_001529"
     CDS             complement(9..251)
                     /locus_tag="VH663_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ8174706.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHIAIPDEDVEDMVFMGDVLDYLVQRLT"
     gene            complement(244..1251)
                     /gene="plsX"
                     /locus_tag="VH663_001530"
     CDS             complement(244..1251)
                     /gene="plsX"
                     /locus_tag="VH663_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEQ8174707.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQMINSGGIKTKIGASLLKSSLYEMKKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            complement(1354..2109)
                     /gene="recO"
                     /locus_tag="VH663_001531"
     CDS             complement(1354..2109)
                     /gene="recO"
                     /locus_tag="VH663_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEQ8174708.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            complement(2295..3257)
                     /locus_tag="VH663_001532"
     CDS             complement(2295..3257)
                     /locus_tag="VH663_001532"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ8174709.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMGIQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            complement(3510..4706)
                     /locus_tag="VH663_001533"
     CDS             complement(3510..4706)
                     /locus_tag="VH663_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ8174710.1"
                     /translation="MKKRILSAVLVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTE
                     QKAAQNQVSALQAQVSSLQSEQDKLTARNTELEALSKRFEQEIKALTSQIVARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEE
                     KQAANQTAINTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATEDKANLVAQK
                     EAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPAAPSAGPQTSYDSSNTYPVGQCTWGAKSLAPWAGNNWGNGGQWAYSAQAAGYR
                     TGSTPMVGAIAVWNDGGYGHVAVVVEVQSASSIRVMESNYSGRQYIADHRGWFNPTGV
                     TFIYPH"
BASE COUNT         1503 a          988 c          734 g         1547 t
ORIGIN      
        1 tattctaact aagtcaaacg ctgaactaaa taatctaaaa cgtctcccat aaataccata
       61 tcctcaacat cttcatcagg aatagcaata tgaaattcat cttcaacatt aataataaat
      121 tcaactagtt caatagagtc aaccgctaaa tcatttttta aatgtgtttg ttctgtaata
      181 gctacactca aatagcttct ttgcttctga attaagttaa tcagcctttc aaaaatctcc
      241 tgtctagtca tttacttgag tctccttagc aaattcttct actaattgtc cgaccacgtt
      301 agtgtctagc atcgtacgta cttgttttat cgttgaaaaa atagctttta catcactaga
      361 accatgagac tttacaacag gtgcctttaa accaaataaa actgcacctc cagcactaga
      421 gtagtcaaga gtcttcttca tctcgtaaag actactttta agtagactag ccccaatttt
      481 agtcttaata ccgccgctat taatcatctg tttcagttgc cccataatgc taatagctgt
      541 tccttctatt gatttcaaca ctgcgtttcc cgtgaaacca tctgatacaa taacatctgc
      601 aacacctgac ataagttctc gcgcctcgac attaccaaca aaagatattg tattatctgc
      661 tgttaaaagt tcataagtag ccttacgaag gggatctcct tttgtttctt ctgtaccatt
      721 gtttaataga ccaacacgtg gattagcaat cccacgtaca ttcttagcat agaaagagcc
      781 taaaatagca tattgatgta aatgttttgc cgtattttca gcattagctc caagatccaa
      841 catatcaaat cctaatccgg ttactgtggg aatagttgat aataatcctg gacgatcaac
      901 tcccttgata cgccccacaa caaacaaacc tacagctaaa agagcacctg tattacctgc
      961 tgaaataatg gcatctgctt ttccttcttt cactgcctta gctgctaaaa ccattgaagc
     1021 cttcttttta cggcgaacag cttttgcagg ttcatcatct gacataattt tttcatctgt
     1081 atgaataata gcaacacgat ctgattgaat aagataggag ttaatctttg tctgatcacc
     1141 gtaaagttga atttcgatat ctgaaaatgc ttctattgct tgattaacac cctcaacaat
     1201 agctttagga gcgttatctc cacccatagc atctattgca attctcttca tttccactcc
     1261 tttaattata tcttgtgttc ttacacaatc tattaatagt atttttatta tagcatattc
     1321 tgaaaccaat tcccataaac gaaagaggcc ctatcatttc ataatatctc cccacttaac
     1381 taaattatca atgaatgttt tactttttaa tttgattcct acatagtcgt gatacaactc
     1441 atcaatgaac tgacgaagct tctttttgat gtctttattc aatgaaatag ttctcaaatc
     1501 atcaaatgtg attttttgaa atcgactcaa caagtagata acatttggat ctaaatgatt
     1561 acgtcggttg tctttgtaat aatgttcaga acaaagtaca gctgaaaaac gatgggaaaa
     1621 atcaagcggt aaatcagtgc gatgacaaat ggcacactca tgaaagttta gactaatacc
     1681 aaaacgatct aatatctgaa tttcaaaaat atttgtcaaa atttcataat ctaggccctc
     1741 ttccatcaaa tcaagtgttt ttttcaaaaa cgtaaacaaa tgcgaatctg attcattatc
     1801 tgcaatcgca gcatcagcta atgctaagac ataactagca taggctaatc gaaaaatatc
     1861 attattaata taccgataag tgttaacgtt actatagtca acaacataag acaagcctgt
     1921 atcatttaac ttgaaaataa aatcagcaat cgttagtggt tgaatgattg aggacatttt
     1981 ggaacgacta atatgtttca cgaaaaacat ctgcttacct gctacttcag taaatatttt
     2041 gactaattta tcatcttctc gataattcct attaaaaaga acaatgccta gtgattctgt
     2101 tagttgcata ttacctcgtc gtatttttct tttacctttt atcttagttt acaataaact
     2161 gttattatct ttcttattat acccaaaatc ctttagaatt gcatagttca tcccacaata
     2221 attaatacca cattaccttt acattttatt caagtcaaaa agcccgagat ctattatctc
     2281 aggcttcgtt aagtttaatt gcccatctca aataaaggtg acaacggacg tttttcatga
     2341 atacgaataa tcgcttctgc aaccaaatca gcaattgaaa tttgttcaat tttatcaata
     2401 agacgttctt ttggcagata aatagtatca agaacaatta gtttttcgat agccgaacgt
     2461 tgaatattat caagtgctgg tccagaaaga acgggatgcg tacatgacgc atacacagct
     2521 gttgcccccg cttcagcaag cgcatctgct gcatggcaaa tagtccctgc ggtatcaatc
     2581 atatcgtcaa tcaagataca tttcttgccc gaaacatttc cgataatatt catcacttca
     2641 ctggtgttca tcttatccac actacgacgc ttatcgataa ttgcaattgg tgtttggagg
     2701 aattgtgcta atttacgcgc acgagtcaca ccaccatggt caggactaac aacgacaaca
     2761 tcttcaccca ctaaaccatg acgatcaaaa tagtctgcaa ttaaaggtgc acccatcaag
     2821 tgatcaactg gaatatcaaa gaacccttga atttgagcag catgtaaatc aaccgttaat
     2881 aagcgatcta caccagcaac ttctaacata ttagcaacta attttgaagt aataggttca
     2941 cgagaacgcg ctttacggtc ttggcgtgca tagccgtagt aaggcatcac aactgaaatt
     3001 ttttcagcac tagcacgctt taaagcatcg accataatca aaatttccat taaattgtca
     3061 ttgacaggtg agctagttga ttgcaaaata aagacatgat gaccacgaat tgattcttca
     3121 atatttactt ggatttcacc atctgaaaac tggcgaaccg tagactttcc aagttgaata
     3181 cccattgcag acgcaacttt ttctgctaat tccttgtttg acgataaagc aaacaatttt
     3241 aaatcagaat aagacatttt ttcctccaaa gttttaatcc tataccattt taacgctttt
     3301 caagtgaatt atcaaatgaa attgaaaaac cgttaaagaa tgtattaaat atacctttta
     3361 tcgactttat atacttctct tatattatac ataagcgatt ggccaaacaa ctctcttata
     3421 aattaactat atcgtcaata ctctcagaac tattccacaa taggagtaat caaaaaggtt
     3481 agaacaactg ttctaaccta aataatatgt tagtgtggat aaataaatgt aacacctgtt
     3541 ggattaaacc agccacggtg gtcagcaatg tactgtctac cactgtagtt agactccatc
     3601 acacgaatac ttgaggcact ttgaacctca actacaacgg cgacatgtcc ataaccacca
     3661 tcgttccaaa cggcaatcgc acctaccatc ggcgttgaac cagtacgata accagctgct
     3721 tgagcactat aagcccattg accaccattt ccccaattat ttcctgccca aggagctaaa
     3781 gatttagctc cccatgtgca ttgtccaact ggataagtat tagaagaatc atatgatgtt
     3841 tgtggtcctg cagaaggagc tgccggagcc gtgagtgcag ctggttcaat agcattacta
     3901 ctttgcgctg ccggagtagc ttgtggtgct ggagtaatag cagattttgc tgcttcaaca
     3961 gatgctgctt gttgtgcagc ctgttcttgt gccttaactt tagcagcctg ttcttgtgct
     4021 aaggcttcag cagcagcttt ttctgcagct tctttttgag ctaccaaatt agctttatct
     4081 tcagtagcag atgctaattg gagagctaaa tttgcagttg cagctaccaa attagcttgt
     4141 tgagtacgta atgtattttg gttttcttca gccattgcca tattagcggc aatggtatta
     4201 atagctgttt ggttagcagc ttgcttttct tcaagggaaa ctttatcagc tttttgttgt
     4261 tctaacaatt tagcgttagc agagacagct ctattaattg ctactaaacg gtttacaaca
     4321 tcagaaattg atttagaatt caaaagtgca ttaatataac cagaagtttc attgttttta
     4381 taagcactac gagcttgatt ttttaatttt tcattacgag caacaatttg acttgttaga
     4441 gccttaattt cttgctcaaa tcgctttgaa agcgcctcaa gttctgtatt tcttgcggtc
     4501 agtttatctt gttcagattg tagtgaactt acttgagcct gtaacgctga aacttgattc
     4561 tgtgcagctt tttgctctgt agtcagattt gagataatag aatcttgctt agcaatctta
     4621 gtacttaaat cctccgctcc tacagttgta gctgctccga gggtaacacc acttacaaga
     4681 actgctgata aaattctttt tttcataata aaaaaactac tccttttcga taagacatct
     4741 tatattttac caaaaaaaaa agtatttaat at
//
LOCUS       DAUGFP010000072         4702 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.072, whole genome shotgun sequence.
ACCESSION   DAUGFP010000072 DAUGFP010000000
VERSION     DAUGFP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.072"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            192..3218
                     /locus_tag="VH663_001534"
     CDS             192..3218
                     /locus_tag="VH663_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEQ8174711.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQINSGSQLEQSKESLSLNKRVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTLGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDKAIAKAEKALVTKKATKNGQLLERSINKAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIRQVPT
                     AAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSNPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
     gene            3238..3639
                     /locus_tag="VH663_001535"
     CDS             3238..3639
                     /locus_tag="VH663_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEQ8174712.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAQGRMG
                     VPKAVGIIHIERIFNLQSGANAGKEINSESN"
     gene            complement(3819..4694)
                     /locus_tag="VH663_001536"
     CDS             complement(3819..4694)
                     /locus_tag="VH663_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEQ8174713.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYRTLHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLRESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSSIEVN"
BASE COUNT         1671 a          740 c          895 g         1396 t
ORIGIN      
        1 tattcattag taagcataag atataacaag agaaattacc ttaaaacagg aagtactaga
       61 gtatttaaaa taagtgatag acttggatat tattaggtcg actagtgtcc ttttcaataa
      121 tttaatatcg ttattattag tatcagccaa tcaaatgtgg ataagttggc ttttagaaag
      181 gttttaccac aatgaagaaa catcttaaaa cagttgcctt gaccctcact acagtatcgg
      241 tagtcaccca caatcaggaa gtttttagtt tagtcaaaga gccgattctt aaacaaactc
      301 aagcttcttc atcgatttct ggcgctgact acgcagaaag tagcggtaaa agcaagttaa
      361 agattaatga aacttctggc cctgttgatg atacagttac tgacttattt tcggataaac
      421 gtactactcc tgaaaaaata aaagataatc ttgctaaagg tccgagagaa caagagttaa
      481 aggcagtaac agagaataca gaatcagaaa agcagatcaa ttctggatct caactagaac
      541 aatcaaaaga gtctctttct ttaaataaaa gagtgccatc aacgtctaat tgggagattt
      601 gtgattttat tactaagggg aatacccttg ttggtctttc aaaatcaggt gttgaaaagt
      661 tatctcaaac tgatcatctc gtattgccta gtcaagcagc agatggaact caattgatac
      721 aagtagctag ctttgctttt actccagata aaaagacggc aattgcagaa tataccagta
      781 gggctggaga aaatggggaa ataagccaac tagatgtgga tggaaaagaa attattaacg
      841 aaggtgaggt ttttaattct tatctactaa agaaggtaac aatcccaact ggttataaac
      901 atattggtca agatgctttt gtggacaata agaatattgc tgaggttaat cttcctgaaa
      961 gcctcgagac tatttctgac tatgcttttg ctcacctagc tttgaaacag atcgatttgc
     1021 cagataattt aaaagcgatt ggagaattag ctttttttga taatcaaatt acaggtaaac
     1081 tttctttgcc acgtcagtta atgcgattag cagaacgtgc ttttaaatca aaccatatca
     1141 aaacaattga gtttagagga aatagtctaa aagtgatagg ggaagctagt tttcaagata
     1201 atgatctgag tcaactaatg ctacctgacg gtcttgaaaa aatagaatca gaagctttta
     1261 caggaaatcc aggagatgat cactacaata accgtgttgt tttgtggaca aaatctggaa
     1321 aaaatcctta tggtcttgct actgaaaata cctatgttaa tcctgataag tcactatggc
     1381 aggaaagtcc tgagattgat tatactaaat ggttagagga agattttacc tatcaaaaaa
     1441 atagtgttac aggtttttca aataaaggct tacaaaaagt aaaacgtaat aaaaacttag
     1501 aaattccaaa acagcacaat ggtgttacta ttactgaaat tggtgataat gcttttcgca
     1561 atgttgattt tcaaaataaa actttacgta aatatgattt ggaagaagta aagcttccct
     1621 caactattcg gaaaataggt gcttttgctt ttcaatctaa taacttgaaa tcttttgaag
     1681 caagtgacga tttagaagag attaaagagg gagcctttat gaataatcgt attgaaacct
     1741 tggaattaaa agataaatta gttactattg gtgatgcggc tttccatatt aatcatattt
     1801 atgccattgt tcttccagaa tctgtacaag aaatagggcg ttcagcattt cggcaaaatg
     1861 gtgcaaataa tcttattttt atgggaagta aggttaagac cttaggcgag atggcatttt
     1921 tatcaaatag acttgaacat ctggatcttt ctgagcaaaa acagttaaca gagattcctg
     1981 ttcaagcctt ttcagataat gccttgaaag aagtattatt accagcatca ctgaaaacga
     2041 ttcgagaaga agccttcaaa aagaatcatt taaaacaact ggaagtggca tctgccttgt
     2101 cccatattgc ttttaatgct ttagatgata atgatggtga tgaacaattt gacaataaag
     2161 tggttgttaa aacgcatcat aattcctacg cactagcaga tggtgagcat tttatcgttg
     2221 atccagataa gttatcttct acaatggtag acctcgaaaa gattttaaaa ctaatcgaag
     2281 gtttagatta ttctacatta cgtcagacta ctcaaactca gtttagagac atgactactg
     2341 caggtaaagc gttattgtca aaatctaacc tccgacaagg agaaaaacaa aaattccttc
     2401 aagaagcaca atttttcctt ggccgcgttg atttggataa agccatagct aaagctgaga
     2461 aggctttagt gaccaagaag gcaacaaaga atggtcagtt gcttgaaaga agtattaaca
     2521 aagcggtatt agcttataat aatagcgcta ttaaaaaagc taatgttaag cgcttggaaa
     2581 aagagttaga cttgctaaca ggattagttg agggaaaagg accattagcg caagctacaa
     2641 tggtacaagg agtttattta ttaaagacgc ctttgccatt gccagagtat tatatcggat
     2701 tgaacgttta ttttgacaag tctggaaaat tgatttatgc acttgatatg agtgatacta
     2761 ttggcgaggg acaaaaagac gcttatggta atcctatatt aaatgttgac gaggataatg
     2821 aaggttatca tgccttggca gttgccactt tagctgatta tgaggggctc gacatcaaaa
     2881 caattttaaa tagtaagctt agtcaattaa catctattcg tcaggtaccg actgcagcct
     2941 atcatagagc cggtattttc caagctatcc aaaatgcagc ggcagaagca gagcagttat
     3001 tgcctaaacc aggtacgcac tctgagaaat caagctcaag tgaatctgct aactctaaag
     3061 atagaggatt gcaatcaaac ccaaaaacga atagaggacg acactctgca atattgccta
     3121 ggacagggtc aaaaggcagc tttgtctatg gaatcttagg ttacactagc gttgctttac
     3181 tgtcactaat aactgctata aaaaagaaaa aatattaaat tgatttaagg agaagaaatg
     3241 ataacacaag aaatgaaaga tcttattaat aaccaattag caatggttgc gacggttgat
     3301 gccaaaggtc aacccaatat tggtccaaaa cgttctatgc gtctctggga tgataaaaca
     3361 tttatttata atgaaaatac agatggtcaa actcgtatta acattgaaga taatgggaaa
     3421 attgaaattg cctttgttga tcgtgagcgc cttttaggtt acaggtttgt aggaactgct
     3481 gaaattcaaa cagaaggtac ttattacgaa gcagcgaaaa aatgggcaca aggacgcatg
     3541 ggggtaccaa aagcagttgg tattattcat atcgagcgta tttttaattt acaatctggt
     3601 gctaatgcag gtaaagaaat aaacagtgaa agtaactaaa attaactgta acagtattta
     3661 ggacccttat tttaaacgct aaaaaacaag catactcttt tttacagcat agtgactatg
     3721 ttgtaaaaag gggtatgctt attaggtgct cttaatattt ctagataact gcacctaata
     3781 agttttcaat agacttatag cacattaatt gacttcaatt agttgacttc aatactagaa
     3841 agagaagaac agtgatgttg gtgttcgttt aaagaagtat cgatttccac agtgactgag
     3901 tttatattgt acctttgaca aatcagtcta atagattttt tgcaatgttt ttcagtggta
     3961 gattctctca agcagcaatg tatagttgct ctatgatcaa taccatccat cgaccaaaca
     4021 ttaagctgat taacagacac tatatgagga agttggctga gttcatggtg caaagtgcga
     4081 tagtctatcg aatcaggaac accatctaaa aaaatgttag cagttgctac taactttgga
     4141 agagctttag ataatataaa agaagcaatg gcaattgata ataaaggatc aagaatgtac
     4201 caaggtttcc attttaagat cagtgacaat atgataatgg ctaaccaccc aaggatatct
     4261 tctaaaaagt gaaggcttaa tatttcttca ttttttgtct ggttagagtg aatgatgaag
     4321 ctagcaaatc cattgattat aattgcaaaa atagctaaaa cgaacatacc gtcataatta
     4381 acaatagtag gatgccataa ttttggtatc gtttcaatca ttactaataa tgacccgcta
     4441 ataagtatta gatttgttgt tagtgctcct aaaaggctaa atcgcttata gcctagtgaa
     4501 aaatttggac tctctttttt aacagccttt ctttctaaga tagctgagat accaatagca
     4561 atggcatctc caaaatcgtg gacagcatct gctaaaatag cacccgaaaa gaataatgtc
     4621 ccaaaaataa attcaatgag ggaaaaacta agatttaata taaaaataat ggtgactttt
     4681 ttacttgctg gcataatttt ct
//
LOCUS       DAUGFP010000073         4648 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.073, whole genome shotgun sequence.
ACCESSION   DAUGFP010000073 DAUGFP010000000
VERSION     DAUGFP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.073"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            1..2149
                     /locus_tag="VH663_001537"
                     /pseudo
     CDS             1..2149
                     /locus_tag="VH663_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018223.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
     gene            2146..2883
                     /locus_tag="VH663_001538"
     CDS             2146..2883
                     /locus_tag="VH663_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174714.1"
                     /translation="MNQLLLKNVCKRYPNQPNYALDHINLTVRKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGSIYCADKEISAFSDNEATSFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNVDSSKINDLINNYAKRFNLYEQLEKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEETMMILSEINKTEKVTILMVTHDVLSASYADRVVLLKDGK
                     LHLEIDKEDCGETFYDVISQALSDRGE"
     gene            2885..>4648
                     /locus_tag="VH663_001539"
     CDS             2885..>4648
                     /locus_tag="VH663_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ8174715.1"
                     /translation="MIWSITKSNIKKNFSLYRIYFLATIGLLSIFIAFLNFISDKIIT
                     EKIGDSGQALVIANGSLVFLTVFLVIFLIYFNNFFVKKRSQELGVLAILGFSKKELTQ
                     LLTLENLVILVLSYLVSLLLGPTLYFLAVLVITHLLELTLEVQWFITANEIIESLGIL
                     VVVFLINVITNGLIISKQSLIEFVNFSRKAEKKIKIRKVRAIIAITALLLSYILCLAT
                     VFSSTRNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFVVSLLKGNKKRLYRPLSNI
                     IYPKFNYRIATKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGIERLTPSAIEYNVES
                     ENGQVNVTTILENDQVSLVDVDLLRLNTNPEVTITESGQTIPYFDIINYSDYKELMKA
                     QGRINSIEGSKSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVSTHNVFSFSTSVTTL
                     VVSDKLYAKLSSCFPEKEMTIRTFNGNSIRSSEAFYNQFSMVPDVISSYSKEYTVKTA
                     NIATYIFITFLSILFIICTGSILYFTSLIEIMENKEEYGYLSKLGYSKKMIHRILRYE
                     TGILFLIPVFIGIVNGGMLLIY"
BASE COUNT         1621 a          619 c          853 g         1555 t
ORIGIN      
        1 tgaaaatcag ttatttgaaa ctataatgtt tctttcaatg aaaacactag ttctagatat
       61 aaatcatttt tccaaggaaa ttgaaaacaa gtcaaaggca tatgaacaat atatacagca
      121 gataagggaa gaaaatggga taagtaattt ttttgacaga tatccatatt tgctaaaaca
      181 aatcaataga gaagttggat tggtagtgga atcatactct ttgctatttg ataggttttt
      241 aaaagatttg tctgaaataa ggtcatgctt taatattact gagtccttaa gtaatgttga
      301 attttcactt ggagacagtc attcccaaaa acaaacagtc gttaagattg aatttaaagg
      361 gaaatccgtt tactaaaagc ctaaaagcta tgattcatac aacatcttgt tggaacttat
      421 tagtctattg aaatctaata atataccttc gttcagtctt cctgaaagct taattaaagc
      481 ggattattgt tggcaacttg gtgtagctta tacaagttcc aataaagatg aagtagcgaa
      541 aatttacttt aagtatggtg ttttagcagc attttctgaa atattttcta ttacggactt
      601 acatatggaa aatgtgattg tttcaggcgg agatttatat ttaattgacg ttgaaacctt
      661 ttttcaaaga aagttgaatg tacaaaatca gaattttgaa gggattactg tagacaccta
      721 tcagagaatt tataagacat cactttcaaa tggtttattt cctgtacaat ttgagaaaaa
      781 cagcgctcct aacgtttctg ggataagtgg aaaaggtggg aaaagaaaaa agggaaagta
      841 tgaactgata aataaaaatc gtggagatat gaagttagtg aaaactgact attttcagga
      901 agatggttat aatattccga cattaaatgg gaaagtggta gagcctcttg actatgctaa
      961 tgagatcatt tcaggattta gagagtgcta catatttctt ctatcacaac gttcaaagat
     1021 taaaggaata gtagaaggtt ttccagaatt gaaaagcaga gccctatttc gaaatacttc
     1081 cgactatgga aaatttttgc aagcttctac gaatccaaag tatcttttct cagaaaaaaa
     1141 gaggaagaat ttattttcga tactatatga ggcaaaacat attgaacatt ttattgttga
     1201 taatgaaatt aaagatttga tgaatggtga tattccttac ttttcaatgg ataccagagg
     1261 aaacgtctat aattctgtag ggacattaat tggaaatcta ggagatacaa cctcgttatt
     1321 tgatagtata actattttga acgatgaaag attgaagttc acttgtgaat tgcttgaaat
     1381 agtctagaaa aaaccgatta agcattggga gagggaaaag ggcaaatctt atcagttttt
     1441 atcaatctcc tcagaacaca attttagtga ggaaatctta gacagcatta gacgaatatt
     1501 tattgatgca gataagaata gcttttcttc agaggaggag ataacatggc tcaatattga
     1561 tattacagag actgagcaat gggtaatatc tcctcagaac attaccttct ctaaaattga
     1621 taaaactgat tttaggaata tatcggcttt tgtatctcaa gattctccaa tatttgatgg
     1681 ggatgtgatg tataacattt cgctaggaag agaatctgtt acagaaaaac aagttattga
     1741 aacctgtaaa agggtatcac tatatgagga tatcaggagt atgccaatga agtttcatac
     1801 cccacttttt cgagacaatc catcactatc tggggggcaa aaacaacgaa tttctctagc
     1861 aagagagtta gtaactaccc ctagaatctt agttcttgat gaacctacat cagctttaga
     1921 tgtaaaaact gaaagaataa tccaaaaaaa tgttgaggct ttacattgta cgagggtttt
     1981 ggttacccat agacttaata cagttgaaaa agctgataag attttaataa tggataatgg
     2041 gaaaattatt gactatggta gtcatcattg tttatataaa aataatgagt actatcgtga
     2101 tttatatgat tcgtacatga acaactatca ggaggaaggg ataaaatgaa tcaattacta
     2161 ttgaaaaatg tttgcaaaag gtatccaaac caaccgaatt atgctttaga ccatatcaat
     2221 ttgactgtta gaaaaggaga atttgtagct gtcatgggac gtagtggtag tggaaaaaca
     2281 acacttttaa atgttacttc aattattgat aaaattgata gtgggagtat ttattgtgca
     2341 gacaaagaaa ttagtgcatt ttctgataac gaggcaacta gttttagaaa aaatgatatt
     2401 ggttttgttt ttcaagatta tatgctgtta gacagtctaa ccattaggga gaatatttct
     2461 gttgctctat ctttaaaaaa tgttgattca tcaaaaatta atgatttgat aaataactat
     2521 gcaaaaagat ttaatttata tgagcagttg gaaaaatatc cgtatcaact ttctggaggt
     2581 caaagacaga gggtcagtat tatcagagca atcattaaag aaccagagat tatttttgct
     2641 gacgaaccaa ctggagcact tgacttgaaa tcttctgagg aaacaatgat gattttgagt
     2701 gaaatcaata aaacagaaaa ggtaactatt ttaatggtta ctcatgacgt tttatcagct
     2761 tcttatgcgg atagagttgt acttctcaaa gatggtaaac ttcacctgga gattgataaa
     2821 gaggattgtg gagagacgtt ttatgacgta attagtcaag ccttatccga tagaggagag
     2881 taatatgatt tggtccatta caaaatctaa catcaaaaaa aacttttcgt tatatcgtat
     2941 ctattttcta gctacgattg gtttattaag tatttttata gcttttctaa attttatctc
     3001 agataaaatc attacagaaa aaattgggga tagtggtcaa gctctagtta ttgctaacgg
     3061 gtcattagtt tttttgactg tatttttggt gatattttta atttatttca ataatttctt
     3121 tgtaaaaaaa cgtagtcaag agcttggagt cttagcaata ctagggtttt caaaaaaaga
     3181 attaacacaa ttactgactt tagaaaatct tgttattcta gttttaagtt acttggtaag
     3241 tttactattg ggaccgacct tatatttttt agcggtactg gtaattactc atctattgga
     3301 attaacattg gaagttcagt ggtttattac agctaatgag attatagagt ctttaggaat
     3361 attagtcgta gtttttctga ttaatgtcat cacaaatgga cttatcatta gtaaacagtc
     3421 tttgattgaa tttgttaatt tctcaagaaa ggctgagaaa aaaattaaga taagaaaagt
     3481 cagagctatt attgctatca ctgcattgct attgtcatat attttatgtt tggcgacagt
     3541 gttttcatcc acacgaaata tgctattaag catagggatg gtaccggttt ctctattgat
     3601 aattgtctta gttgttttag gaacagtgtt caccatcaga tatggattgg cttttgtagt
     3661 ttcgttatta aaaggaaata aaaaaaggtt ataccgtcct ctgtctaata tcatctatcc
     3721 caaatttaac tatcgtattg caacaaaaaa taaattatta acagtcttgg gaggtctttt
     3781 aacagtaacc gtttcagttg ccggaatgat ggtaatgctc tatgcttatt ctcttaatgg
     3841 gatagagagg ttgactccat ctgccataga atataatgtt gaatcagaaa acggtcaagt
     3901 caatgttaca actattttag agaacgacca agtgagcttg gttgatgttg acctattgag
     3961 attgaacact aacccagaag taactatcac agaatctggg caaacaattc cttattttga
     4021 tataattaat tacagtgatt ataaagagtt aatgaaagct caagggagaa taaattctat
     4081 tgaaggtagt aagtcactcc cattgttaat aaattattat ccaacagaaa ttagccttgg
     4141 aaaaaccttt aacttaggaa atgcatatga tgttactgta aaacaagtat caacgcataa
     4201 tgtttttagt ttttctacaa gtgtcacgac cttggttgtt tctgataaat tatatgctaa
     4261 acttagttct tgttttccag agaaagaaat gacaattagg acttttaatg gaaattcgat
     4321 taggtcaagt gaagcatttt acaatcagtt tagtatggtt cctgatgtta tcagtagtta
     4381 tagtaaggaa tacacagtaa agactgctaa tattgcgact tatatcttta taactttcct
     4441 atccatactc tttattattt gtacaggtag tattctgtac tttacaagcc tcatcgaaat
     4501 catggaaaat aaagaagaat atggctatct aagtaagcta ggttatagta aaaaaatgat
     4561 tcatcggatt cttcgatatg aaacaggtat acttttcctt attcctgtat tcattgggat
     4621 tgtaaatggt ggtatgttgc ttatttac
//
LOCUS       DAUGFP010000074         4586 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.074, whole genome shotgun sequence.
ACCESSION   DAUGFP010000074 DAUGFP010000000
VERSION     DAUGFP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.074"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            64..1878
                     /gene="glmS"
                     /locus_tag="VH663_001540"
     CDS             64..1878
                     /gene="glmS"
                     /locus_tag="VH663_001540"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEQ8174716.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSRESTYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLTTTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2074..2409
                     /locus_tag="VH663_001541"
     CDS             2074..2409
                     /locus_tag="VH663_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEQ8174717.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            2516..3157
                     /locus_tag="VH663_001542"
     CDS             2516..3157
                     /locus_tag="VH663_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ8174718.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            3167..3796
                     /locus_tag="VH663_001543"
     CDS             3167..3796
                     /locus_tag="VH663_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ8174719.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQNVDAKEKALDLLTRLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            3812..>4586
                     /locus_tag="VH663_001544"
     CDS             3812..>4586
                     /locus_tag="VH663_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ8174720.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKSLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQDKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     ALLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQSGK"
BASE COUNT         1425 a          762 c          977 g         1422 t
ORIGIN      
        1 tataccaatt attgaaatta tgagagaaag tagcaagtta acgaacttgt taaggtaatt
       61 aagatgtgtg gaattgttgg agttgttgga aatcgcaatg caacggatat tttaatgcaa
      121 ggccttgaaa agcttgaata ccggggttat gattcagcag gaatttttgt ggctaatgcc
      181 aatcaaacaa acttgattaa atcagtgggg cggattgctg atttgcgtgc caagattggc
      241 attgatgttg ctggttcaac agggattggt cacacccgtt gggcaacgca tggccaatca
      301 acagaggata atgcccatcc tcacacgtca caaactggac gttttgtact tgttcataat
      361 ggtgtgattg aaaattacct tcacattaaa acagagttcc tagctggaca tgattttaag
      421 gggcagacag atactgagat tgcagtacac ttgattggaa aatttgtgga agaagacaag
      481 ttgtcagtac tggaagcttt taaaaaagct ttaagcatta ttgaaggttc ctatgccttt
      541 gcgttaatgg atagccaagc aactgatact atttatgtgg ctaaaaacaa atctccattg
      601 ttgattggac ttggtgaagg ttacaacatg gtttgttcag atgccatggc catgattcgt
      661 gaaaccagtg aatttatgga aattcatgat aaggagctag ttattttaac caaagataag
      721 gtaactgtta cagactacga tggtaaagag ctgatacgag attcctacac tgctgaatta
      781 gacttatctg atattggcaa agggacttat cctttctata tgctgaaaga aattgatgag
      841 caaccaaccg taatgcgtca attaatttca acttatgcag atgaaactgg taacgtacag
      901 gttgatccag ctatcattac ctctatccaa gaggctgacc gtctttatat tttagcggca
      961 gggacttcct accatgctgg ttttgcaaca aaaaatatgc ttgagcaatt gacagataca
     1021 ccagttgagt tgggagtggc ttctgagtgg ggttaccaca tgcctctgct tagcaagaaa
     1081 ccaatgttta ttctactaag ccaatcagga gaaaccgcag atagtcgtca agttttagta
     1141 aaggcaaatg ctatgggcat tccgagtttg acagtaacta acgttccagg atcaacctta
     1201 tcacgtgaat caacatacac catgttgatt catgctggac ctgaaattgc tgttgcatct
     1261 acaaaagctt acactgcaca aattgctgcc cttgcctttt tggctaaggc agttggtgag
     1321 gcaaatggta agcaagaagc tcttgacttt aacttggtac atgagttgtc attggttgcc
     1381 caatctattg aggcgacttt gtctgaaaaa gatctcgtgg cagaaaaggt tcaagctttg
     1441 ctaactacta ctcgtaatgc tttttacatc gggcgtggca atgattatta cgttgcgatg
     1501 gaagctgctt tgaaattaaa agagatttct tatattcaat gcgaaggctt tgcggctggt
     1561 gaattgaaac atggaaccat ttcattaatt gaggaggaca cgccagtaat cgctttaata
     1621 tcgtctagtc agttggttgc ctctcatacg cgtggtaata ttcaagaagt tgctgcccgt
     1681 ggggctcatg ttttaacagt tgtggaagaa gggcttgacc gtgagggaga tgacattatt
     1741 gtcaataagg ttcatccttt cctagccccg attgctatgg tcattccaac tcaactgatt
     1801 gcttactacg cttcattaca acgtggactt gatgttgata agccacgtaa tttggctaaa
     1861 gctgtaacag tagaataaat gattataatg gataaggcaa ccatctgtta ggtggttgct
     1921 ttttgttttt aggagaatag cagagcttag aaaatggcct tgagaaaagc tatacaagaa
     1981 gaatagtcat cttctgatga gaacgtattc atcttgtcaa atcattgatt ctaaagtaaa
     2041 atggtaacat taagaaatgt ttaggagtgt aagatgtcat taccaaattg tttgcaatgc
     2101 cagtctgaat atgtttacga agatggtctt ttattaatct gtccaatgtg tgcttttgaa
     2161 tggacaccag gcgaagaagc gactgaagaa gaaggacttg ttgttttaga tagcaatggt
     2221 gtccgactgt ctgatggtga taccattact gtggttaaag atttgaaagt aaaaggcgct
     2281 ccaaaagacc tgaaacaagg gactcgtgtt aaaaatattc gcctggtgga aggtgatcat
     2341 aacattgatt gtaagattga tgggtttggc gcgatgaaat tgaaatcaga atttgtcaag
     2401 aaaatctaat aataatacgc aaccagctga tctggttgta tttttataaa aaaacctctc
     2461 cccaaatacg ttcaaatttg gtataatagt aaaatattca gttttaggag gcaacatgac
     2521 ctatattcag caagttttac ccagcttatt agatggtgcc ttggtgacct tacaagtatt
     2581 ctttattgtt atcattcttt ctataccctt aggggctatt ttagctttct tgatgaagat
     2641 tccctttaaa ccgctacagt ggtttttgac cctatacgtg tggatgatgc gagggacacc
     2701 attactactt caattgattt ttttctatta tgttttgcca agtgtgggga ttagttttga
     2761 tcgaatgcca gctgctattt tggcgtttac tttgaattat gctgcctact ttgctgaaat
     2821 ttttagaggt gggattgagg ctattccaaa aggtcaatat gaagcagcta aagtattaaa
     2881 gttaaaacct cttcaaacca ttcgttatat tattttgcct caagtgttta aaattgtgtt
     2941 accaagtgtt ttcaatgaag tcattaattt ggtcaaagat tcttcccttg tctatgtact
     3001 cggtgtagga gatcttttat tagcaagtaa gacggcagcc aatagggatg caaccttagc
     3061 ccctatgttt atcgctggtc ttatctattt gcttttaatt ggactggtca cgattatttc
     3121 aaaacaagtt gaaaaacggt ttaattatta tcagtaagga ggcttcatgt tagaactcaa
     3181 aaatatttcc aaacagtttg gtcaaaaaac catttttgat ggctttaatc taaccgttca
     3241 agacggagaa gtgctctctt tggtaggtcc atcaggtggc ggtaagacaa cactcttacg
     3301 gatgttggct ggtcttgaat ctattgattc aggtcaagtc ttttacaatg gagaagatgt
     3361 cggtattgat catttggaaa atcgtaactt gcttggattt gttttccaag attttcaatt
     3421 gtttcctcat cttaccgttt tagacaacct aacgctgtct ccaacaatta caatgggcaa
     3481 gcaaaatgtt gacgctaaag aaaaggcttt ggatttactg acacgtttag gattaaaaga
     3541 acatgcccaa gtttatccct attcgttatc tggaggtcaa aaacaacggg ttgctttggc
     3601 gagagccatg atgattgatc ctcaaattat cggttacgat gaaccgacca gtgccctaga
     3661 tcctgaatta cgtcagacgg tagaagcctt gattgttcaa aaccgtgaaa tgggaataac
     3721 tcaaattgta gtcactcacg atcttgtttt tgccgaagcc atctcagatc gtattattag
     3781 ggttaatcct aaatagaaga ggacccaaaa gatgattata aaaaaaagaa ccgtagcaat
     3841 tttagccata gctagtagct ttttcttggt agcttgtcaa gctactaaaa gtcttaaatc
     3901 aggagatgct tggggagttt accaaaagca aaaaagtatt acagttggtt ttgacaatac
     3961 gtttgttcct atgggctata aggatgaaag cggcaaatgc aaaggttttg atattgattt
     4021 ggctaaagaa gtttttcacc aatatggact caaggttaac tttcaagcta ttaattggga
     4081 catgaaagaa gcagaactaa acaatggtaa aattgatgta atctggaatg gttattcaat
     4141 aactaaggag cgtcaggata aagttgcctt tactgattct tacatgagaa atgaacaaat
     4201 tattgttgtc aaaaaaagat ctgatattaa aacaatatca gatatgaaac ataaagtgtt
     4261 aggagcacaa tcagcttcat caggttatga cgccttgtta agaactccta aactgctgaa
     4321 agattttatt aaaaataaag acgctaatca atatgaaacc tttacacaag cttttattga
     4381 tttaaaatca gatcgtatcg atggaatatt gattgacaaa gtatatgcca attactattt
     4441 agcaaaagaa gggcaattgg agaattatcg gatgatccca acgacctttg aaaatgaagc
     4501 attttcggtt ggacttagaa aagaagacaa aacgttgcaa gcaaaaatta atcgtgcttt
     4561 cagggtgctt tatcaaagtg gcaaat
//
LOCUS       DAUGFP010000075         4464 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.075, whole genome shotgun sequence.
ACCESSION   DAUGFP010000075 DAUGFP010000000
VERSION     DAUGFP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.075"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(283..646)
                     /locus_tag="VH663_001545"
                     /pseudo
     CDS             complement(283..646)
                     /locus_tag="VH663_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1067..1600)
                     /locus_tag="VH663_001546"
     CDS             complement(1067..1600)
                     /locus_tag="VH663_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEQ8174721.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            complement(1680..2456)
                     /gene="recX"
                     /locus_tag="VH663_001547"
     CDS             complement(1680..2456)
                     /gene="recX"
                     /locus_tag="VH663_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEQ8174722.1"
                     /translation="MKITKIEKKKRLYLIELDNDESLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIADIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIEDLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            2571..3926
                     /gene="rlmD"
                     /locus_tag="VH663_001548"
     CDS             2571..3926
                     /gene="rlmD"
                     /locus_tag="VH663_001548"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ8174723.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGENIFC
                     QITAVKRNFAEAKLLTVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLANFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
BASE COUNT         1344 a          715 c          929 g         1476 t
ORIGIN      
        1 actttggtta aagtaatcga actaacttgg ttaattgtat tttaatgata aaaagcttaa
       61 atttattata caacaaattt aagcttttta ttgtttcttt ttaattgcag attgaggttt
      121 aaaagacact ttacaatgtt ctcaagtcat gtgttgttgt aggttgtatg cacatattac
      181 aaatcccatt atagaagaaa ttgtttaatt aatggatagt aatggatggt atggagatct
      241 taatgtggtt ttaatggtaa ttttagcagt tatagtaaga caaaaggtga cttggaaaag
      301 cttatgcaga cgattcttaa ctcccacaat atcttctgtc agattttatt agacttcttc
      361 tcagtgtcaa ttttgtctgt tttacgaacg tgcaggctat tcaattgctt cttagcttct
      421 aaaggattaa accgagtata agcgtagcca ttatctgcga aaaaacctga agacgatgag
      481 aatagacatt aatcgcttta aggataatct cagggttatg gacggttttc aagtttctaa
      541 gaagacgatt gaggccgatg gtatcgtttt gtcttgaatg attccttatt ttagtggttt
      601 cattttcaat acacgacgtc aagcgtccta catactttga taaaattcta cctaaaagag
      661 gtgacttgtc agttttgatt acaacatcaa gtgccaaaag actttgcgac ttaacaagac
      721 acttctactt taacgcaaag aaaaagtagt gagtactctc tttcgtcgga gatttcctcg
      781 ctactaaact tagtattttt tgatactttc cttaggtagt gcttaaggca gcgactttct
      841 ttggaattcc atgccttatt tttgagatcc taggctcaaa aatgccaagt ggcgtcaaca
      901 ttaacattga cgccactttt accacaacga aaaacatctg ttttggctca ctccacacgc
      961 agaaatagga aattggatgt caaatcagca gagctgttca tgctttttga gcctaaaact
     1021 gagtttgtct gcgttctgag cactccttgg gagtgctgga caactcttaa cgattcttca
     1081 gttcaagata acgtttgtac caaatagtga tataggcctt ggaaaatggt cctttttcat
     1141 gattaatcca atcaaccaat attttgacat tttcttttag gataaagtcc atatcggctg
     1201 aatactgcat ctctttttta tgaatctcgt attcatcaac atcaagtaat ctcttttcac
     1261 catccgcaaa gactttgaca tctaaatcgt aatcaatgta tttaagggcc tcagtgtcca
     1321 tcatgtatgg actagctaag ttacagtaat aagagacgcc attatctcga atcatggcta
     1381 taatgttaaa ccagtatttt ttatgaaagt aaacgattgc aggttcgcgg gtcacccatc
     1441 gtctaccgtc actttctgtg acaagagtgt gatcattaac tccaatgagg gcattttcag
     1501 ttgtttttag taccatagta tcgcgccaag tacggtgcaa actaccatca tgcttataac
     1561 tttgaattgt aataaagtcg ccttctttag gtaatttcat gccttaccaa ctttctacaa
     1621 ttaaacttat cctctatagt ctatcataaa tgtgtgaaaa aatcacttct tttgtcagtc
     1681 tataaataat ttcttaactt gcaattaatg tcgtcgctgt tgtagccttt tcggtagaga
     1741 gcctgataga gcttttgctt taaggtataa ccatcatatt tgcgactgag tttacgatat
     1801 tgtttgtcta attctttgtc aagaagatct tctatttctt gattatcttg gtcaaaatta
     1861 aggtgattga ggctatgttt agctagatcg taagaaaagc ctttggtcag taatgcttgg
     1921 gtgattttat ctttcaaggc ttttggtggc aatttttctt gatatttgtc aaagagtttt
     1981 tggcttactt tttgagcgag ttggctaaag tcagtttgag ataagatagg atcaatgtca
     2041 tgacttgcaa tgcctttttg taataatttt tgttttaaga cttggggacc tttatcacca
     2101 tttaactgat tttggcgaat gtaggtatca gccaatttgg tgtcgtctat ccattgttct
     2161 tcttggagtt gagtgatgat gtcagcaata atgtgttctt caatctcatg cttgcgcagg
     2221 taatcagcaa cttgcttgtt gctgcgttgt tgaaaggaaa gaaaataaag ggctaaattt
     2281 ttgccgtagg acagttgggc aaaatgtttc atgtcttcaa gctgatcatt gtcaaggact
     2341 ttatctttac tcaacataaa ccgaacaata gtatcttctg ttacataaag ggattcgtca
     2401 ttatccaatt cgataaggta gaggcgtttt ttcttttcaa ttttagtgat tttcatactt
     2461 tgattatatc aaaaatggta aaatagaaga tgaaaatact gctaaaaatg tgttgcttgt
     2521 gaatgctttt ggtggtgaga ttaggatatt agaggaagta aggggaagag atggtcgtaa
     2581 aggttaaaca gaaaattcct ttaaaaatta agcgaatggg aattaacggt gaggggattg
     2641 gtttttatca gaaaactttg gtatttgtcc ctggtgcttt aaagggagaa aatatttttt
     2701 gccaaatcac ggcggttaaa cggaattttg cagaggccaa gctgttaacg gtaaacaagg
     2761 cttctaagaa tcgtgtcaaa ccagcttgtt ctgtttatga aacttgtggt ggttgccaaa
     2821 tcatgcatct agcctatcct aaacagctag atttcaaaga tgatgtgatt aggcaggccc
     2881 ttaaaaagtt caaaccaact ggctatgaac aatttgagat tagaccgact ttaggaatga
     2941 aaaagccaga tcactatcgg gccaaattgc agtttcagtt gcggtcattt ggcgggacgg
     3001 tcaaggctgg tttattttcc cagggaagtc atcgcttggt tcctattgac aattgcttgg
     3061 tacaggatca actaacgcaa gatattatca ataagattac gcagttagtg gacaagtaca
     3121 agttgcccat ttacaatgag cgcaagattg ctgggattcg gacgataatg gttcgtaagg
     3181 cacaggctag tgatcaggta cagattattg ttgtgagtag caaagaagtt cggttagcga
     3241 atttcattgg cgaattgact aaggcatttc ctcaggttaa aacggttgcc ttgaatagta
     3301 atcgttctaa atcgagtgag atttatgggg acgaaacgga aattctatgg ggacaagagg
     3361 cgattcatga agaagtttta gactatggat ttgcgttatc accgagagct ttttaccaac
     3421 ttaatccaca gcagacagaa gttttgtatg gtgaggtggt gaaggccttg gatgtaggga
     3481 gcaaagatca tattattgat gcctattgtg gggttggctc gattggcttt gcttttgcag
     3541 ggaaggtcaa gtctgtgcgg gggatggata ttatcccaga agcgattgaa gatgcacaaa
     3601 aaaatgccaa agccatgggc tttgacaacg cttactatga ggcagggaag gcagaggata
     3661 ttattcctaa atggtataaa caaggttacc gcgcggatgc catcattgtg gacccaccaa
     3721 gaactggttt agatgataaa ttattaaaaa cgattcttca ttaccagcct aaacagatgg
     3781 tttatgtgtc ttgtaatacc tccactttgg cccgcgactt ggtgcagcta acaaaggttt
     3841 acgatgttca ctatatccaa agtgtagaca tgttccccca tacagctagg accgaggctg
     3901 tcgtgaagtt acaaaaacgt gtatgattga gtggcaataa caactcaagt tttataagct
     3961 caggtaatgg gtttgagcaa tagcttgaca tagtagggat gtcaaaaacg gtttactaag
     4021 catagtcata ttattgataa ctctatgttt attttagaaa cattcatggt taaaaaagtt
     4081 tgttagtttg tcactaagta gggtctattt gaagagcaag tacacacaga caatattagg
     4141 ggaattgatt gttgaaaaat agctgttttt tgctataaat tagaaaactg agtggtcagg
     4201 aggttacatt accagaagtt attttagtat gctaatatga acgatgatat agaggattta
     4261 tcctgattta atcttttaag ctgtaagaaa cctttgcaaa aactgtaggc tgtgtgagtc
     4321 tttatgtgtt agatattaaa gataaatgca acactttttt ataatgagat caacaactga
     4381 taaatctgag ttgataacca gtaaaatgat cggaatatag aaaagttact taatcaagct
     4441 tttcgtaatt taactgttag aatg
//
LOCUS       DAUGFP010000076         4406 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.076, whole genome shotgun sequence.
ACCESSION   DAUGFP010000076 DAUGFP010000000
VERSION     DAUGFP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.076"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..565
                     /gene="xerD"
                     /locus_tag="VH663_001549"
     CDS             <1..565
                     /gene="xerD"
                     /locus_tag="VH663_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEQ8174724.1"
                     /translation="STANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINTDIF
                     YQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLSQIL
                     IPFLEQQLVGKEVYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFILKEK
                     LAGKSIIELSDILGLKSPMTLEKYYKS"
     gene            565..1269
                     /locus_tag="VH663_001550"
     CDS             565..1269
                     /locus_tag="VH663_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEQ8174725.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKNNHTV
                     IERDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNKVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            1266..1817
                     /locus_tag="VH663_001551"
     CDS             1266..1817
                     /locus_tag="VH663_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEQ8174726.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            1939..2661
                     /locus_tag="VH663_001552"
     CDS             1939..2661
                     /locus_tag="VH663_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ8174727.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            2658..2921
                     /gene="yidD"
                     /locus_tag="VH663_001553"
     CDS             2658..2921
                     /gene="yidD"
                     /locus_tag="VH663_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEQ8174728.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            3099..3647
                     /locus_tag="VH663_001554"
     CDS             3099..3647
                     /locus_tag="VH663_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEQ8174729.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHIYEHDKLK"
     regulatory      3756..3880
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3959..>4406
                     /locus_tag="VH663_001555"
     CDS             3959..>4406
                     /locus_tag="VH663_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8174730.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVT"
BASE COUNT         1459 a          711 c          889 g         1347 t
ORIGIN      
        1 atcaacagct aatcaattct tatattactt gtatcaaata aagtatttaa atagttattt
       61 tcgattaaca gataccatga aagtgatgcg gactgagaag cagcaagctg ctattattaa
      121 tactgatatt ttctatcaaa aaaccccttt tgtgtggggg caattaatca gcttattaat
      181 attggagctg ggtttaactc cgagtgaagt agctggtatt gaagtagcta acttggattt
      241 aaattttcaa atgttgacgc taaagactaa aaaaggtgtg cgtgtattac ctttgtcgca
      301 aatactaatc ccttttttag aacaacaact cgttggcaaa gaagtctatc tttttgaaca
      361 tcgaggcatc cctttttcaa gacaatggtt ttttaatcac ctaaaaacct ttgttcgaag
      421 tattggttat gaaggattga ctgctcaaaa attacgagag caatttattt tgaaagaaaa
      481 actagctggt aaatcgataa tagaacttag cgatattctt gggctgaaaa gccctatgac
      541 cttagaaaaa tactacaaat cataatggat ataaaactta aagattttga aggtcctctt
      601 gatttactct tgcacttggt atcacagtac aaagttgaca tctacgaggt tcctattgtt
      661 gaggttattg agcagtacct taattatatt gaaaccttac aggtaatgaa acttgaagtg
      721 gctggtgact acatgctgat ggcaagtcag ctcatgttaa tcaaaagtag aaggctatta
      781 ccaaaggtgg tagaacacat cgaagaagaa gatttagaac aagatctgtt agaaaagatc
      841 gaagaatata gtcgctttaa agcagttagt caagcgttag cgaagcaaca tgaccaaagg
      901 gcaaaatggt attcaaaacc taaacaagaa ttaatttttg aagatgctat cttacaggaa
      961 gataaaacgg tgatggatct atttctagct ttttcaaaca taatggcagc taaaagagca
     1021 gttttaaaaa acaaccatac agtcatcgag cgtgatgatt ataagattga agacatgatg
     1081 gcttctatta aacagcgact ggaaaaagaa aacgttatta gcttatcagc gatttttgag
     1141 gaatgccaaa cattaaataa agttatcagt atatttttag ctagtctaga attgattaag
     1201 cttcacgtgg tatttgtaga gcagctctct aattttgggg ctatcatact acgaaaggag
     1261 aagaaatgac gtatttgtca cagatagaag cgctgctatt tgttgcaggt gaagaaggat
     1321 taagtttgcg acatctagcg tctatgttat cattaacgcc aacagctctt caacagcaat
     1381 tagaaaaact ctctcaaaag tatgaaaaag atcaacattc tagtctttgc ttgatagaaa
     1441 cagctaatac ctacaggcta gtaactaaag aaggttttgc agaactcttg cgtgcttatg
     1501 ccaaaacacc aatgaaccaa agtttatctc gtgcgagttt agaggtcttg tctattgttg
     1561 catacaaaca acctattaca cgaatagaaa ttgatgacat tcgaggggta aactccagtg
     1621 gagctttgag taaattattg gcatttgatc tcattagaga agctggcaaa aaagatgtgg
     1681 ttgggcgtcc tcatttgtat gcgacaacag attattttct ggactatatg ggaattaatc
     1741 atttagacga attgattgaa gtatctgccg ttgaaccagc agatgaagag attgctcttt
     1801 ttaggacaca agactgagtt ttgcttgtca tcaaatagca aatggcgttt acaaaaggat
     1861 ttgttttaag gcgttttagt tgaaaaaaag ccttaaacta gggacaacaa aaagttgctc
     1921 aaaagaaaga agaatataat gcgaattaac aaatacattg cccatgcggg tattgccagt
     1981 cgtcgtaagg ctgaagaatt gattaagcaa ggcttagtaa ccttaaatgg tcaggttatt
     2041 acggatttag caaccactgt aaaatcggga gacgtggttg aaatagaagg aagtcctatt
     2101 tacaacgagg aaaaggttta ctatttgctt aataaacctc gtggcgctat ctcaagtgtg
     2161 tcagatgaca aaggccgtaa aacggtactg gatttactac ctcaggttaa agaacgcata
     2221 taccctgttg gacgattgga ttgggacaca tcaggggtcc ttattttaac gaatgatggt
     2281 gattttacag atactatgat tcatcctaga aatgaaatag acaaggttta tctggcgcgt
     2341 gtcaaaggaa ttgcaacgaa agaaaatctt cgtcccttaa ctcgaggaat tgttattgat
     2401 ggtaaaaaga ccaaaccagc tcgctacaat attgttcgtg tagaagctga taagaatcga
     2461 tcgattgtag aattgactat ccatgaaggg cgaaaccatc aggttaaaaa aatgtttgag
     2521 tctgtggggc ttttggttga taaattatct cgtacacgtt ttgggaccgt cgacttaaag
     2581 ggactaagac caggagaagc aagacgctta aataaaaaag aaataagtca actgcataac
     2641 ttagctaata cgaaaaaatg atgaaaaaac tattaatagt atctgttaaa gcttatcaaa
     2701 aatacatttc tcctttatct cctcctagtt gtcgctacaa gccaacctgt tcagcttata
     2761 tgttaactgc tattgaaaaa catggtacta agggaatact aatggggata gcaagaatat
     2821 taagatgtca cccttttgtt gctggtggtg tcgatcctgt tccagaagat tttagcctaa
     2881 tgagaaataa aaacacgtct aaaaacgctg aaaaggcatg actttatcaa aaaatggctt
     2941 gacattgata gctgaagtgt tataatggaa aacaagcatt cgttggttta aatcaaacct
     3001 gttatgattt aagttagcga gtcaccatca accacattgt ttgctgagca tgactccgag
     3061 cagtgtggtt ttttaaatta aaaagagtaa atgagattat gacgacaaaa gaacttatca
     3121 acaaaaacga caaagtaaaa aaggctcgca atcatattgt cctgtttcaa ccccaaattc
     3181 cacaaaatac tggtaatatt gctagaacat gtgctgcaac taatgcacct ttacacatta
     3241 ttaaacccat gggatttcct attgatgacc gcaaaatgaa gcgtgccggc ttggattact
     3301 gggacaagtt agaactgcat ttttatgatc atttagaaca gtttatcaat caatgtcacg
     3361 gacagcttca cctgattagc aagtttgcag ttaataatta ttctcaagct acttatgctg
     3421 atggcgattc ccattatttc ttgtttggac gagaagatac aggcctgcca gaagatttta
     3481 tgcgagaaca tgctgaaaaa gcacttcgta ttccgatgaa tgatgagcat gtacggagtt
     3541 taaatgtctc aaatacggtt tgtatggtga tttatgaagc ccttagacaa cagggctttc
     3601 aagggttaga gttaaagcat atttacgaac acgataaact aaaataatgg actttttaaa
     3661 agcgtcagtt tttggcaaca tgcgttgtca gtagctgacg cttttaaaaa tttagtcaaa
     3721 gctattgcta tctaggttta aatctgctat gctagaagtg tcttcagggc agggtgtgat
     3781 tcccgaccgg cggtgactag gtaactagaa gtccgcgagc gcaagctgat gtggtgtaac
     3841 tccacaaccg acagtatagt ctggatgaga gaagaccggg ctttttgtgt aggcttattt
     3901 tagctacaca ataacgacgg aacttctttc aatttgaaaa aattggagga attttttaat
     3961 gtcaaaaaca cataaaatga ttatgattgg tatactttct gcgatatcat ttctattgat
     4021 gctagttagc tttgccatta ttccaggcgc agctttttta aaaattgaat ttagtattat
     4081 tcctgtctta tttggtttaa tgattatgga cttaaagagt gcttacttga ttttgttatt
     4141 gaggtcctta ttgaaactct ttttgaacaa tcgtggggtt aatgatttta ttggtctccc
     4201 aatgaatatt atcgctatcg ctttatttgt gacggctttt gccttagttt ggaaccgtca
     4261 aaaaacgctt agtcaatatg tatttgctag cttactaggc acagggttat taacgtttgg
     4321 catggttgtt ctcaattata cttttgccat tcctttatat gctatatttg caaatattga
     4381 tatcagagct tatattggtg ttacta
//
LOCUS       DAUGFP010000077         4294 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.077, whole genome shotgun sequence.
ACCESSION   DAUGFP010000077 DAUGFP010000000
VERSION     DAUGFP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.077"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            51..479
                     /locus_tag="VH663_001556"
     CDS             51..479
                     /locus_tag="VH663_001556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174731.1"
                     /translation="MKTKSKRFLKLATLCLVLLGSTLLVTYPVKATETPLSGYESTEQ
                     ALPKSNQVTETLHSEGDLQKIAEKSDDGYKLGYQKGYEAGRKEDASENPDENKLESKS
                     LDYISGYYAGYYRGWDETNKPFQTLLEDIYRWLWEIFFKP"
     gene            1002..1460
                     /locus_tag="VH663_001557"
     CDS             1002..1460
                     /locus_tag="VH663_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174732.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLTTQPVKAEEGQNKQQEVSVS
                     SQSGQSEGEDLSEDSDDKYPYAEGHEAGQDFGYKEGLKAGWQEADPKEPKDIPEPEFN
                     PYESEGEDRNDFKEGFRDGYPAGYVEGWRKSHPLWAIVQDVLPWLYNFLR"
     gene            complement(1542..3040)
                     /locus_tag="VH663_001558"
                     /pseudo
     CDS             complement(1542..3040)
                     /locus_tag="VH663_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612207.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3237..4053)
                     /locus_tag="VH663_001559"
                     /pseudo
     CDS             complement(3237..4053)
                     /locus_tag="VH663_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1336 a          769 c          837 g         1352 t
ORIGIN      
        1 tacgagtagt aaactataac tatatcaaaa tgatggaatg gagtgatttt atgaaaacaa
       61 aatcaaaacg ctttttaaaa ctagcaaccc tttgcttggt ccttttggga tcaaccttac
      121 tagtgacata cccagtaaaa gcgacagaaa cgccattaag tggatatgag tctactgaac
      181 aagctctacc taaatccaat caagtaacgg agaccttaca tagtgaaggg gatttacaaa
      241 aaattgcaga aaaaagcgac gatgggtata agcttgggta tcaaaagggg tatgaagctg
      301 gtcgaaaaga agatgcttcc gaaaatccag acgaaaataa attagaatct aagtctcttg
      361 attatattag tggttattac gcaggatatt atcgagggtg ggatgaaact aacaaacctt
      421 tccaaacatt attagaagat atttatcgtt ggttatggga aattttcttt aaaccgtaag
      481 taaatgttaa ttattcatta aaatatagtc ataaaatgtg ataacatcta tttattatag
      541 ttattttact tgaggaagat agtaccttgt atgcatgcag aagcatatta taaagatctg
      601 ctataattgg gatagtccct ttagtagtca ggccacctct gataggggtg gcttttttgg
      661 gttttagtga cccgtgtgtt gagaaaggag catttatgcc agaaaagaaa gtgttgcccg
      721 tccgttgcct aatatgaacc gacagttatt ccctattaaa atagacgcaa atatccacgc
      781 tttcactgta gttgctagta aggtctaaca caaaacatca aaggctttca aaaacaactc
      841 ctaagcttaa aaaaggctgt ttaacagatt tgttgagctt aagggagatt gccatgattt
      901 tctaatcatt tgatgtttaa aatgataaaa acatgaactt gaaatgaaag actgctagta
      961 gtaaactaag cttatcaaaa aaatgaaaat ggagtggttt tatgaaaaca aaatcaaaac
     1021 gctttttaaa cctagcaacc ctttgcttgg ccctactagg aacaactttg ctaacgacac
     1081 agcccgtaaa ggcggaggaa gggcaaaata agcaacagga agtgtctgtc agcagccagt
     1141 ctggacaatc tgaaggagaa gacttatcgg aggattcaga cgataagtat ccatatgctg
     1201 aaggtcatga agctggccaa gattttggat ataaagaagg attaaaggca ggttggcagg
     1261 aagctgatcc taaagaacca aaggatattc ctgaacccga atttaatcca tatgaatctg
     1321 aaggagaaga ccgtaatgat ttcaaagaag gatttcgcga cggatatcct gcaggatacg
     1381 ttgaaggatg gagaaaaagt catccattat gggcaatcgt tcaagatgtc ttgccttggc
     1441 tttataattt tttaagataa aatagtgtat aatactagcc ctgacagttc tggcctacgc
     1501 ctactatgac ctacctaaac gacatccaaa gagtgaggga actacgaggc tgggcaaaaa
     1561 ctagcttcga cataaaacca cactgcagtt ctttccatct tgaaaatact caagatgaaa
     1621 gaattacgtg tggtttttga tttttaaaag ttttagtaac taaattcttt gccaattttg
     1681 tgagattcat gctcatgaag agacatccga cctccgtttg gacgaattaa ttgcctcaaa
     1741 cgtgaactct gcgtacgcta aaaatactct tcaatctacc aaaaacgggt tcgacatcga
     1801 ttttccgttt agcgtagata tgagagccct cttcgctatg caattgttcc tttatgagtt
     1861 ccttaaaata attccaagtc ggattataat ggatttgttt ctgacgatca ctttctgttt
     1921 tagccagtta ttctagctct ggcgtgtcct gtattttatc tgcctcgtaa attttgaaat
     1981 ctcgtttaaa cccgtacttg tcgactcgac gagagtaaga tttaaaagaa tagacaatcc
     2041 catcaggttt tataatctgg tctgtctctc caagatagtc ccagttagct gggttctccg
     2101 cactgttctt atatttctga gtcaattcct tttggtacat gccataggga ataagagctg
     2161 ttttctccaa ctcatcaata ataaaacgat aattttcttc gctaccataa cctgcatcgg
     2221 ccataatatt ttggaataag tccaatgttt gattggattg gaggaagggt cttagggttc
     2281 tgatatcggt tgggtttgca aagatatcat aagcaaggat atattgacca ttagtcgctg
     2341 cttgaacatt ataacctggt ttcagctgac cattcttcat atgactcgct tttatatttt
     2401 aaggcttgtg aagaaatagt ctctcccaga ctatttcttt cctttattcg cataaaggta
     2461 gcgtcaggat cagttctaga aaaagagttg cgttcttgaa gaatttctct cgcctcctca
     2521 tatcgctgtt tacgaggaat ataatctttc ctcaactgat tgcggaattt cttgatgcgc
     2581 tgccgttttt gtgtattaac cgaactacct tttatcactt ttggttcttg agtgatcgct
     2641 ttttctactt catcaagggc ttctttccct ttttcaagca gcacgttaag tccttcgcta
     2701 gtttcatatt cttcttttga aagggctaaa gttacccctt cttgaatgag cttatcatag
     2761 agttaagaaa tgtttccatt gtctctcaag agcaaggtaa aataaataaa agtagtctta
     2821 atcaggttat tggcaggctt actcgaacgg tcttattgat cgttttatag ctaacataag
     2881 tgtcttgact tagccatttc atcggaataa cttcttcgtt catctgcacc attttacgac
     2941 cagaaaagac ctgacgacta taggcaaaga gtgtcatttt taaaagcata gcaagatgaa
     3001 aagcaggtcg gccagtatga gacttttctt caagtaaaat caacgctgaa gactttttgg
     3061 attcccataa ttgttaacta catcatttta gagcaatcat atagagacta agacataatt
     3121 ttaccaaagt tttgaaggct attgaagagt aactttcttc tttaatatat ttaataaatt
     3181 ttcgcaagtt gtcaagtcaa gtgcaacgag ttcatagata actagagttc cagaggttaa
     3241 gctgttcggg ctagctcaaa caggaatgtt gaggattgat attgaagaag tcgtcttggg
     3301 cgttcattga tggctgtaag ataattgtca aattcttcag aggttagtga attaagagag
     3361 attcctttag ggacatattc tctgaggagg ccattgaagt tctcgtttgt tcctctctca
     3421 tgtgaagaat agggatgtgc aaagtaaacg tcaacagctt ctaaggttga gagtaaacta
     3481 aactcagaac cattatccgc tgtggtggat gcaataggat actgactgat gagatgcgtg
     3541 acagcacggt taatggtttg ggactgcttg tcttctagct taactcctag tgcatagcgt
     3601 gtctggcgct ctaccaaagt caacataaca gcttcacctt tggttttctt gccgagaacc
     3661 aaatcaatct cccaatgccc aaattcagaa cgatcattaa tatattctgg acgttcttcg
     3721 atagatttac ctaaagtctt cttattcgtt ttcatgactt tcttagaacg ttttctgata
     3781 ctaaccatct taggcaaatc gattggtttc atagacacgt tgtctactat cagcgaaata
     3841 ggcgttgaag aaggtttgtt ttccattctt atcctttacc tgatctactg aaccacgttt
     3901 tatttctcgg cagattgtag aacggtgact acccagaagt cgagcaatct caacgggttt
     3961 ctttcccatt ttaagataag cagcaatttc accgcgttct gtggctgaaa gatgagaata
     4021 ggaggatttt ctggtaaaat taatattaga catgttcatc tgctttccat actgaattgg
     4081 ggaattctag tatatcagac gaacatgtct tttttgttgc acttcatttt acaacgcggg
     4141 aaaatataaa atatatcttt gagcaaggta gttattcttg aaaatttgag aatatattgt
     4201 taaagacatc aaaaaacagt acctatcaag atacagtcgt atttctttaa attatgctaa
     4261 aaactgcaga tgataaacat ttattaaact attc
//
LOCUS       DAUGFP010000078         4024 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.078, whole genome shotgun sequence.
ACCESSION   DAUGFP010000078 DAUGFP010000000
VERSION     DAUGFP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.078"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            101..1099
                     /gene="ruvB"
                     /locus_tag="VH663_001560"
     CDS             101..1099
                     /gene="ruvB"
                     /locus_tag="VH663_001560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEQ8174733.1"
                     /translation="MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            1237..1674
                     /locus_tag="VH663_001561"
     CDS             1237..1674
                     /locus_tag="VH663_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEQ8174734.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     NPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMNKEY"
     gene            1698..2099
                     /locus_tag="VH663_001562"
     CDS             1698..2099
                     /locus_tag="VH663_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174735.1"
                     /translation="MIDMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            2096..3871
                     /locus_tag="VH663_001563"
     CDS             2096..3871
                     /locus_tag="VH663_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEQ8174736.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLLWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNFSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMIGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGSLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPKGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
BASE COUNT         1230 a          630 c          833 g         1331 t
ORIGIN      
        1 ctatctttta aagtagttca agagcttagt gctgttgact ggtggcgatt ataataaaag
       61 taggccactg ctctctttta ctttatgtta taatagtcct atggctagaa ttttagacaa
      121 taatgtaatg ggaaatgagg agttttcgga tcggactctt cgccctcaat atctacacga
      181 atatattgga caagataaag ttaaggaaca attcgctatt tttatagaag cagctaaaag
      241 acgtgacgaa tctttagacc atgttttatt atttggacct ccaggactgg gaaaaacaac
      301 aatggcattt gtgattgcca atgaattggg agttaattta aagcaaactt caggtcctgc
      361 cgttgagaaa gctggagatt tggtagctat tttaaatgaa ttagaaccag gggatatttt
      421 gtttattgat gaaattcacc gcatgccaat gtctgtcgag gaagtactat atagtgctat
      481 ggaagacttc tatattgata ttatgattgg cgctggagat actagtcgta gtattcattt
      541 ggatctgcct ccatttacat tgattggtgc tactacacgt gcaggcatgt tatctaatcc
      601 cctcagagct cgttttggta tcactggtca catggaatac tatcaagaaa aggatttgac
      661 agagattgtg gagcgtacag ctactatttt tgaaataaaa atagaccatg aagctgcacg
      721 gaaattagct tgtcgcagta gaggaacgcc ccgcattgct aatcgattac taaaacgagt
      781 gcgtgattac gctcaaatta ttggtgatgg catcataacg gctcaaataa ctgatagagc
      841 tttgactatg ttagatgttg accgcgaagg tctcgattac attgatcaaa aaattttacg
      901 aacgatgatt gagatgtatc agggaggacc agttggtttg gggacattat ctgtgaatat
      961 tgccgaggag cgtaatacag ttgaagagat gtatgaaccc tacttaattc aaaaaggctt
     1021 tctcatgcga acacgaacag gtcgtgtagc gacgcaaaag gcttatcgtc atcttggcta
     1081 tccctatcaa aacacttaat gatttttcaa atctaagaaa attaatctga acctgctttt
     1141 tgtgttcgga ttttttgata agttgacaga aaattattag attgatttct ccgatatcag
     1201 ttagaaattt gatataataa ttcaaaggag tgtttaatga aaaaagtatg ttttgtctgt
     1261 ctgggtaata tttgtcgaag ccccatggct gagtttgtaa tgaagtcaat agtatcaagt
     1321 gatgttatga tgatagaaag tcgtgctact tctgattggg agcatggaaa ccctattcac
     1381 tcaggtacac agtccatatt aaaaacttac caaattaatt atgatataac taaatgctcc
     1441 aagcagataa ctataactga ttttaacacc tttgactata ttattggtat ggatagcgat
     1501 aatgtgaaaa atctcaaaga gatgtcgcaa catcaatggg actctaaaat ctatctgttt
     1561 agagaagggg gtgttcctga tccttggtac actaacgatt ttgaagaaac ctaccaatta
     1621 gttagaaaag gatgccagga ttggctttct cgtttaatga ataaagagta ttgataatta
     1681 gaagttgagt aaaagaaatg atagatatgc aagatatagt aaaaaaatgg agtattacac
     1741 gggcgaagtt ggaaattgtc tcggtgattg tcatattggt ttgtgcaatt tctgttttta
     1801 gcgtaagaat ttcgaataag acaagtttga cctatgataa agggcgtatg cactatactg
     1861 gttacgtgat taaccataaa atgaatggag aagggaagct tgtctacccc aacggtgata
     1921 tctatgaggg tacttttaag aatggcctat ttgaaggaaa aggaactttt actgctaaga
     1981 cgggctggtt atataacggg gagtttcata aaggacaagc aaatggcaaa ggtgtgttaa
     2041 aagcaaagaa taataaagtc tataaaggga tttttaaaca ggggatattt caaaaatgag
     2101 aataaaatgg ttttcgtttg ttagggtaac aggactttta ctggttttgc tctaccactt
     2161 ttttaaaaat gtatttccag gaggttttat tggtgttgat attttcttca ccttttcagg
     2221 atatctaatc acagccctct tgattgatga atacacaaaa aaagagtcta tcgatattat
     2281 cggtttttta aaaagacgct tttatcgaat tgttcctcct ttggtgttaa tgattttatt
     2341 gaccattcct tttacgtttt taattaagaa ggatttcatt gctaatatag gaagtcagat
     2401 tacagctgtt ttaggattta ccactaatat ttatgaaata ttgacgggaa gtagttatga
     2461 aagccaattt attccacatc tctttgttca tacgtggagc ttagctattg aagttcattt
     2521 ttacttgttg tggggtgtat ttgtttggtt attagctaga cgcaaggaga ctcagaaaca
     2581 actgagagga ctactgtttt tgatttcttt gggaatattt gctattagtt tcttgagcat
     2641 gtttataaga tcttttatga cctcaaattt ttccctgatt tatttttcga gtctttctca
     2701 cagttttccc ttctttttag gggcgatgtt tgccacaata acaggtatta acgagactac
     2761 tgttcgattt caaaaaaatg tacgtttatg gccgcgccag tatgtccttg ctgctatgat
     2821 tggagcattt acacttttgc ttgtgctaac ggtcacattg gattttaacc atatcacgac
     2881 ctatctattt ggctttgctt tggctagctt attcgcatca attatgattt atgcagcgcg
     2941 tgttcttcat gagcaaacac cagatgtaca agagcctaaa gctatcactt atatagctga
     3001 tattagctat gggatttacc tcttccattg gccattttac attattttta gccaattaat
     3061 gtctcatatt ttggcagtta ttctaacagt tttcttttca atcttgtttg ccactgtgtc
     3121 ttactatatt gtggaacctt tggtccaagg gagaaaacca aacctgcttg gtttagaaat
     3181 tgactgttct ccttactata agtggattgt aggagggtct ctcgccttag cattattaac
     3241 tttaggaact tgtatgatag cgcctaaagt tggtaagttt gagaaacaat tgttggtcag
     3301 ttccctacaa caggctcaaa gtaatatgga gcggacacat acactagctg caggagacgc
     3361 taatgctttg agtgacgtag gtattattgg tgattctgta gcattacgct ctagtgcagc
     3421 attctcaaaa attatgccac aagcgcaact ggatgcagcg gtcagtcgga attttaaaga
     3481 agctttcgat ttatttaata accaaattaa gtctaagagt ctatctaaga ctgttgtctt
     3541 agcagtaggt gttaactcgc ttgacaacta ttctcaagca gttcagtctt ttattgaggc
     3601 attgccaaaa ggacatcgtt tagttttggt ttctccatat aatgccaaaa atgccagtca
     3661 ggttgcagaa gctagagact atggtcttaa attgtccaaa aaatacaagt atgtcactat
     3721 tgcagactgg tataaagttg ctgttgaaca tccagatatt tggtatggca gtgacggtgt
     3781 tcattatagt gaagatagtc aaggagcaga actatatgtc tccacaatcc agactgctgt
     3841 tgagaagtca gctaaaaaac cagctaaata aagttaagaa atgtctaaga gataagtcag
     3901 atccattgtg ctctggcttt ttcttgtgaa caaatgatga tggtaaaata gtagtacttt
     3961 gaggttttta aaagaaagcg gtttcagaaa caaaacggct ttttttagat atatttctga
     4021 aaaa
//
LOCUS       DAUGFP010000079         3091 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.079, whole genome shotgun sequence.
ACCESSION   DAUGFP010000079 DAUGFP010000000
VERSION     DAUGFP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.079"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..201
                     /locus_tag="VH663_001564"
     CDS             <1..201
                     /locus_tag="VH663_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ8174737.1"
                     /translation="KIAQGGTQIYVNYSSVEQLKINIPTLKEQEAIGNFFQTLDQQIA
                     QSEEKLTELKALKQTLLNRLFV"
     gene            214..1794
                     /locus_tag="VH663_001565"
     CDS             214..1794
                     /locus_tag="VH663_001565"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEQ8174738.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLLAVCDNLEKHFNTFTEAQKIFEDAYQDEGLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGREDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDSHIKKILDAYKSRDNFDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(2346..2951)
                     /locus_tag="VH663_001566"
     CDS             complement(2346..2951)
                     /locus_tag="VH663_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ8174739.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDIILLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
BASE COUNT         1054 a          577 c          476 g          984 t
ORIGIN      
        1 aaaattgctc aaggtggcac tcaaatctat gtaaattatt catctgttga acaattaaag
       61 ataaatatac ctacattaaa agaacaagaa gccattggta atttcttcca aactttagac
      121 caacaaattg ctcaatctga agaaaaacta acagaactca aagcactcaa acaaacatta
      181 ctcaatcgtt tattcgtata agaaaggacg cacatggcag agaaaacaac ttcactccgg
      241 caagcactat ggcattcggc cgaccaatta cgtggtcaaa tggatgccaa tgactataaa
      301 aactatcttc ttggactcat cttttacaag cacttatctg ataaattact cttggctgtt
      361 tgtgataatc tagaaaaaca cttcaatacc tttacagagg ctcaaaaaat ctttgaagat
      421 gcttatcaag atgaaggcct aaaagatgac ctcatttctg ttgttactgg tgatttaggt
      481 tactttatcg aaccaacact gacctttgaa aaactcatcc aagatgttta tcacaatact
      541 ttccaattag aatcattagc tcaaggtttt cgcgatattg aacaaagtgg tgaagacttt
      601 gaaaacttat ttgaagatat tgacctctac tctaaaaaat taggctctac tcctcaaaaa
      661 caaaatcaaa ccatttctaa tgtcatgaaa acattaaatg aaattgactt tgaggcagta
      721 gatggggata ctttaggcga tgcttatgag tacttgattg gtgaatttgc cagtgagtca
      781 ggaaaaaaag caggtgagtt ctatacccca caagctgtct ctcacttgat gactcagatt
      841 gtctttttag gtcgtgaaga tcaaaaagga atgaccctct atgaccctgc tatgggatct
      901 ggatcactcc ttttaaatgc taagaaatat agtaaccaat ccgataccgt ttcttactat
      961 ggtcaagaaa tcaatacttc tacttataac ttggctcgta tgaacatgat gcttcatggc
     1021 gttgccatcg aaaaccaaca cttaagtaac gctgatacct tggatgcaga ctggccaaca
     1081 gatgagccaa ccaactttga tggggtgttg atgaacccac cttattcttt gaaatggtca
     1141 gcgactgctg gtttcttaac agatccacgg ttttcaagtt atggtgtatt agcaccaaaa
     1201 tcaaaagctg actttgcctt tttacttcat ggtttctatc atttaaaaaa tacaggaacc
     1261 atggcgattg tcttaccaca tggagttctt ttccgtggtg ctgcagaagg caaaatccgc
     1321 caaaaattac tagaacaagg ggctatcgat accattatcg gactcccatc aaatatcttt
     1381 tataatacaa gcatccctac gactattatc atattaaaga agaacagaac caacaaagat
     1441 gtcttcttta tcgatgcctc aaaagagttt gataagggca aaaatcaaaa cacaatgaca
     1501 gatagccata tcaaaaagat ccttgatgct tataagagtc gtgataattt tgataagttt
     1561 tcatatttag cctcatttga tgaaatcatt gaaaatgact ataacttaaa tatcccacgc
     1621 tatgttgata cctttgagga agtcccagtt aaaccactgc cagaattagc taaacagctc
     1681 agtgacattg atcaagaaat tgccaaaaca aatgccaaac tagatcaact catgaaacaa
     1741 ctagtcggaa caaccaaaga agcacaagac gaattggata cttttagaaa ctaaaagaca
     1801 aaaaaacacc acagtagatg attactactg tggtgtttta tttaaaattg agtagtgaac
     1861 ctttattgag tacttatcat acaagattct ccttcttcac ctataaatca aaatagaatc
     1921 atctcatcta actttgtccg tttctttgtt ttatttaaaa tagttatttg aagttacaat
     1981 gatttctcaa acgtttagaa agcttaaaca cggttaagag aaattttcag gacaactttt
     2041 gatccttcca caaaaaccat ggtgcgaacc ataaagtagt tccaatcgtt gatatataag
     2101 catatattaa cgttctggga gttgtgaagc tttacgagtt tttttagacc tggtttaaac
     2161 aacttttgtt tgactaaatt taaaaagctt ttcatatcag gcatttccct actttttata
     2221 aaagcgttat ctcaatcaat ttcaatcaaa aagaactcaa aaacaatgag tttaaatctg
     2281 agtatttaac aggaaatccc tagcaacgac acaagtaaaa cttatgtcgt ttgattatct
     2341 gcaactcaga aatccataaa ataccctaat tgttcggctt tgtagatagc ctcttgctta
     2401 ttatttacca aaagctttga gtagatgtgg gtcaaatgat taaatactgt tctcctacta
     2461 atttgtaacg tttcctctat ttcttttata gagaatcctt tcttactcaa atttaagata
     2521 gctatttcct ggtcagttaa agattcaaca taatcctgag attcgatttg ttcaaaatac
     2581 ttttttccag aatccacttt ttttaagata gaaatgagtt gtttagggtc tatattttta
     2641 tctacaaaac cgtatgctcc tacttttttt gccctctctc tatagattga tttaacatat
     2701 cctgtcaaca taacaacttt caaatgagga gtagactgta tgagttcttt tgctatctcc
     2761 aatccatttt ctttagaaat atttgttaaa ttaatatcaa gcaatataat atcgtactta
     2821 gacaaatcaa tcgtcacatc gttaaaatga gaagtaatcg tatctatgac atctacttca
     2881 tcatactgtt ggaataacag ttggatactc tttgcaaata gtctgtggtc atcaattaat
     2941 aaaatcttca taacacaatt tccaatctat gggaagtaat attctgatag taaattgttt
     3001 agtatccatt tgtagttcta gatctccatc aaaagctgct agtgtttctt gaaatgattt
     3061 caatcctctg ccataactta tagagtgtac t
//
LOCUS       DAUGFP010000080         2673 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.080, whole genome shotgun sequence.
ACCESSION   DAUGFP010000080 DAUGFP010000000
VERSION     DAUGFP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.080"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            16..687
                     /gene="sdaAB"
                     /locus_tag="VH663_001567"
     CDS             16..687
                     /gene="sdaAB"
                     /locus_tag="VH663_001567"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEQ8174740.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGVSIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
     gene            699..1571
                     /gene="sdaAA"
                     /locus_tag="VH663_001568"
     CDS             699..1571
                     /gene="sdaAA"
                     /locus_tag="VH663_001568"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEQ8174741.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKKQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIESQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(1985..2599)
                     /locus_tag="VH663_001569"
     CDS             complement(1985..2599)
                     /locus_tag="VH663_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEQ8174742.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKEKAKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
BASE COUNT          833 a          496 c          496 g          848 t
ORIGIN      
        1 aaaacggagt tgcctatgaa tacacaaaaa tttcaatcag tttttgacat cattgggcca
       61 gtaatgattg gtccatcaag tagtcacaca gctggtgccg tcagaattgg taaggtagtc
      121 cattccatct ttggagatat tcctgatgaa gtgacgtttc acctttataa ttcatttgct
      181 aaaacctaca gaggacatgg tactgataag gctcttgttg caggaattat gggtatggat
      241 actgataatc ctgatattaa aaattctcta gaaattgctc atcaaaaagg gataaaaatc
      301 tattgggata tcttaaaaga tagtaatgct ccccatccca atactgttaa aatcagtgtc
      361 aaaaaagctg acaaaacact tagtgttact ggtgtttcaa ttggaggagg aaacattcaa
      421 gtaacagaac taaatggttt ttctgtctcg ttatccatga atactcctac tatagttact
      481 gtacacaaag atattcccgg aatgatcgca aaggtaactg atatcctatc ttccaataat
      541 attaatatcg ccacaatgaa tgttacacgt gaatctgctg gtgaaaaagc tactatgatt
      601 atagaagtcg attctagaga gtgccaagaa gcagccaacc aaattgcaaa aattcctcat
      661 atttataatg tcaacttctt tgactagaaa ggaaagctat gttttatact attgaagaac
      721 ttgtcaaaca agctgaccaa caatttaatg gaaatattgc tgaactcatg attgccacgg
      781 aagtggagat gtctggaaga aatcgagaag atattatcaa aatcatgtca cgcaatttac
      841 aagttatgaa agcagctgta acagaaggac taacctcaac caaatctatc agtggtttga
      901 cagggggcga tgcggtcaaa atggataact acattaaaaa agggaacagc ctttctgata
      961 ctactattct taatgctgtt agaaatgcta tagctgtaaa cgaattaaat gctaaaatgg
     1021 gacttgtctg tgcaacaccc actgcaggaa gtgcaggatg cttaccagca gtgctcgcaa
     1081 cagctattga aaaacttgac ttatcagaaa aaaaacaatt agagttttta tttactgcag
     1141 gagcttttgg tcttgttatt ggaaataacg cctctatttc tggtgcagaa ggtggatgcc
     1201 aagcagaagt tggttctgca gctgctatga gtgcagctgc tcttgttaaa gcagctggtg
     1261 gaacatctca tcaagctagt caagctattg cgtttgttat caaaaattta ttaggacttg
     1321 tctgtgatcc tgttgccggc ctagtagaag tgccttgtgt caaacgcaat gccctaggag
     1381 caagtttcgc ccttgtcgca gctgatatgg ctttagctga tattgaatct caaattcctg
     1441 ttgacgaagt tattgatgct atgtatcaag tcggctctgc catgccaact gctttccgtg
     1501 aaacagcaga aggtggttta gccgctactc caactggacg acgttacagc gtagaaatat
     1561 ttggcgaata aatagatagt tagaaaaatt cttagaaata tttgaaagcg ttttattttt
     1621 ctgctatata ttcactaaaa tgactaatag atagctatca agctatgttg ttttccctta
     1681 gaaacaactc ctagtaaatt gttaaaactc aaaagtgtag tagtgtaaaa tactgaaaat
     1741 tgctaatgac cttataaaca tttaaaagtt tttttcctgc ttgttatcag tataactgcc
     1801 ttaaatatca gaggcactta agtgctggtc agttttccta aataatacga gctatattcc
     1861 ctgataaggc aatgttttgt tttgagacta attcatcact acaaatcata aagacaatta
     1921 aataccaaaa acctattgtt agtaaatact aacaataggt tttatttctt ccagctatgt
     1981 ttatttagta accccaagct gataaacctt gtgcactata ggctttcaag gctgcattga
     2041 cttgatcttc aaccgttgct gtagaacccc aacctggcat tgtctggaac aaccctgatg
     2101 ccccagaagc attagctgcg ttaggatttc cattagattc acgcgcaatt atatgttccc
     2161 aagttgattg tggaacacct gttgctgctg ccatctgtgc cgccgcttga cttcctacaa
     2221 taccagcagt atttccattg gaaagtacca cattgtttgg attataggct actgaagata
     2281 cctgctgaac agtttgagtc acttcttgct gaacaaccgg tgcttcctct gttactgtcg
     2341 gagctacaga gttttctact gtgggaatag gctccgaagc aatgctcttt gctttttctt
     2401 tgctttcttc tttagtaata gcatgattat tttttggagc cttgtcgatt gttaccattt
     2461 tactttctga cttcttagca taagacttag tatcagcatt tttacttgag aaggcaaaaa
     2521 tgatggctaa tattgttaga gctaacgcta ctaatccaaa attaaaataa cgttgtttta
     2581 aattttcttt cttaaacatt caataatacc tcacttttct ccgttatcca taatatcttt
     2641 taattattac ctcgttattg aagatatatt aaa
//
LOCUS       DAUGFP010000081         2526 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.081, whole genome shotgun sequence.
ACCESSION   DAUGFP010000081 DAUGFP010000000
VERSION     DAUGFP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.081"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            7..420
                     /gene="yaaA"
                     /locus_tag="VH663_001570"
     CDS             7..420
                     /gene="yaaA"
                     /locus_tag="VH663_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEQ8174743.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRVGDKISLPDQDLIIIIVEPSQEEKEQFAEEMAEKTRVAALVKQMNQANKK
                     TSSKHNNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
     gene            422..1528
                     /gene="recF"
                     /locus_tag="VH663_001571"
     CDS             422..1528
                     /gene="recF"
                     /locus_tag="VH663_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEQ8174744.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLAGYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH"
     gene            complement(1584..2447)
                     /locus_tag="VH663_001572"
     CDS             complement(1584..2447)
                     /locus_tag="VH663_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEQ8174745.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIVGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
BASE COUNT          891 a          494 c          421 g          720 t
ORIGIN      
        1 atgacaatga tatacaaatt atttacagaa tttatcaccc tacaagccct tcttaaagaa
       61 ttaggaatta tccaaagtgg tggcgctatc aagggatttc tcgctgaaac aaccgttcta
      121 tttaatggtg aagatgagaa acgtcgaggc aaaaaaatca gagtagggga taagatttct
      181 ctacctgacc aagacttaat tatcattatt gttgagccta gtcaagaaga aaaagaacaa
      241 tttgccgaag aaatggctga aaagacacgt gtagcggctc tagtgaaaca aatgaaccaa
      301 gctaacaaaa agacttcttc taaacataac aatcgtcaat ctactaccaa aaaatcacta
      361 agagcaacta agaaaactaa aggtaaacca actgcacctg tccgcttccc aggtatctag
      421 tatgtggatt aaagagttag agttaaaaca ttatcgcaat tatgaccacc tgcttgcctc
      481 attttcgtcg ggtttaaatg tctttatcgg taataatgct caaggaaaaa ccaattttct
      541 cgaggcaatc tattttctat cacttactag aagtcatcgc acgagggctg ataaagaatt
      601 gattcatttt gatcactcaa ctgtttctct tactggtaag attcagcgta ttagtggtac
      661 tgttgattta gaaatcaatc tatcagataa aggacgtgtg acaaagatta atgcgctgaa
      721 gcaagcaaaa ttatctgatt atattggaac gatgatggtt gtgctttttg caccagaaga
      781 tttgcaacta gttaagggag cacctagcct tcgtcgcaaa tttatcgaca ttgaccttgg
      841 tcaaattaag cctgtttact tatctgagtt atcccattat aaccacgtgc ttaaacagcg
      901 taatagctat ttaaaaagtg cccaacaaat tgatgccgct tttctagctg ttctagatga
      961 gcaattagct ggctatggag cccgtgtcat ggaacatcgt attgatttta tcaatgcgtt
     1021 agagaaagaa gcaaatactc atcaccaagc catctcaaat ggtttagaaa gtctctctct
     1081 ctcctatcag tcgtcagttg tctttgacaa aaaaactaat atttaccaac aatttttaca
     1141 ccaacttgaa aaaaatcatc agaaagattt cttccgtaaa aacactagcg ttggtccaca
     1201 tcgcgatgac ttagcctttt acattaacgg gatgaatgct aactttgcta gtcaaggtca
     1261 gcaccgcagc cttattcttt ccttaaaaat ggctgaagtt agtttaatga aggccttgac
     1321 tggagataat cccattcttt tactagacga tgtcatgagc gagctagaca atacacgaca
     1381 aacaaagtta cttgaaacag taattaaaga aaatgtccaa acctttatca caacaacaag
     1441 tttggatcac ctgtcacagt tgccagaagg tatccgtatt ttccacgtga caaaaggaac
     1501 cgttcaaatt gattctgata ttcactaata acatcaaaaa agggttcagt taaactgagc
     1561 ctttttttga tattatgtgg cgattaagat ttgaccacac ctaataaaat agcacctacg
     1621 ataaaacaaa taattcctgt gacaacccaa cgcatttctt ttttggtttt ggtttcgcct
     1681 aggaataaaa tgcctccaac aatcgaaatg atagcaccta attgtgagaa actaaaggca
     1741 atagcaagtc ctgcttttga tgcagctaaa agcataaaaa tattcccaat gccccaaagt
     1801 aatccaacaa cgctattttt aatcacatac tggtcaatgg aaattttaaa tgacatgaaa
     1861 gttatagctc ctaaaaccat acctactgcc atgggtaaaa tgactgacaa aacttcaaat
     1921 ttcatgatgt tattaaataa tacagcatac atcacgtaac caattgttga ataagtgagc
     1981 gccctaaaac cttttgaaaa attatgaagg tgattgactt gtgcattagc atcatcttgt
     2041 ttactagaaa agtaaaaacc aacaatcaga agtaatagtg ctagacttcc tactacaaat
     2101 tgcataggtc ttgtccattc atgaaaaacg aggacaccaa tcaaacttcc cagaacaagt
     2161 tgagagccac ttgacaaagg attggcaacg gagactccca tgtattgcat ggcatgaaat
     2221 tgaccagttt gaccaattga ccagataaaa ccacccaata ttccaaaaag ccaaagttgc
     2281 agtgtcatct caggtctaac aattaaccaa acagctaatg aaaataataa agccccaaaa
     2341 gtcattccta gagtttgttg cgaaggtttt cctccaattt tgttactaac aaatccaatg
     2401 cttccccatg taaacatggg aataagtgca taaaaaatac cttccaaaat gttatctcct
     2461 ttatagtaaa tctttaaaga ttataataat tctatcataa aatggtttca tgtaaagact
     2521 aattat
//
LOCUS       DAUGFP010000082         2480 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.082, whole genome shotgun sequence.
ACCESSION   DAUGFP010000082 DAUGFP010000000
VERSION     DAUGFP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.082"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..259
                     /locus_tag="VH663_001573"
     CDS             <1..259
                     /locus_tag="VH663_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEQ8174746.1"
                     /translation="YLKVSRLIKRRSVAKEVADKGRIKVNGILAKSSTNIKLNDHIEI
                     SFGNKLLTVRVIEIKDSTKKEDALKMYEIISETRITLNEEA"
     gene            246..617
                     /locus_tag="VH663_001574"
     CDS             246..617
                     /locus_tag="VH663_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEQ8174747.1"
                     /translation="MKKPSIVQLNNHYIKKENLKKKFEEEESQKRNRFMGWILVSMMF
                     LFILPTYNLVKSYVDFEKQNQQVVKLKKEYNELSKRTKKEKQLAERLKDDNFVKKYAR
                     AKYYLSREGEMIYPIPGLLPK"
     gene            752..2038
                     /locus_tag="VH663_001575"
     CDS             752..2038
                     /locus_tag="VH663_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEQ8174748.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQLPSNPNLYQETCAYKDSDLTLPVGRLGVNQPLLIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGK
                     ISKIADNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFCSEIKALSGIDWDME
                     QRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHD
                     VAIVYGNTPFILSIFTNKSTYEDITAIADDVYGILK"
     gene            2035..>2480
                     /gene="tilS"
                     /locus_tag="VH663_001576"
     CDS             2035..>2480
                     /gene="tilS"
                     /locus_tag="VH663_001576"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEQ8174749.1"
                     /translation="MMTYQEIFNEIKNKAYFKNHRHVLIAVSGGVDSMNLLHFLYLFQ
                     DKLTIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYA
                     FFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSV"
BASE COUNT          952 a          347 c          413 g          768 t
ORIGIN      
        1 atatctaaag gtatcgcgcc ttattaaacg tcgttcagta gcaaaagaag ttgcggataa
       61 gggacgaatt aaagttaatg ggatacttgc taaaagttca acgaatataa aactaaatga
      121 tcacattgaa attagttttg gaaataaatt actgacagta agagtcattg aaataaaaga
      181 tagtacaaaa aaagaagatg ctcttaagat gtatgagata atcagtgaaa caaggataac
      241 attaaatgaa gaagcctagt attgttcaat taaataacca ttatattaag aaagagaatc
      301 tcaaaaaaaa atttgaagaa gaagaatctc aaaaaagaaa tcgttttatg ggatggatcc
      361 ttgtaagtat gatgttttta tttattttgc caacttataa tcttgtcaaa agttatgttg
      421 attttgaaaa gcaaaatcaa caggtggtta aattaaaaaa agagtataat gaattgtcaa
      481 agaggacaaa aaaagaaaaa caattagcag aacgactaaa agatgataat tttgtcaaaa
      541 agtatgctag ggcaaaatac tatttatcgc gtgaaggaga aatgatttat cctattccag
      601 gactattacc aaaatgatga tggacaatat tgtaaaaaaa atagaagcat ttcttgcttt
      661 ttctgataaa aaattagcag agctgcaaca ggaaaatcaa aaagttaaag aagaaagtca
      721 gtatattcaa aaaaactaga aaggagtcaa tatgagaaaa ttattagcgg ctatgttaat
      781 gacttttttt ctgactcctt taccagtgat tagtacagaa aaaaaactta tattttcaaa
      841 aaatgctgtt tatcaattga aacaagatgt cgttcaatca acacaattct ataatcaact
      901 accctctaat ccaaatcttt atcaagaaac gtgtgcttat aaagacagtg atttaactct
      961 accagtagga agattaggtg taaatcaacc attacttatt aaatcgcttg tgcttaacaa
     1021 agaatcttta ccggtttttg agttagctga tggtacctat gttgaggcta atcgacaatt
     1081 gatttatgac gatattgtac ttaatcaagt agatatagat agctattttt ggacacaaaa
     1141 gaaacttagg ctttattcag ccccttatgt tttaggtacg caaacaattc cttcttcttt
     1201 ttcatttgct caaaaagttc atgccactca aatggcacaa acaaaccatg gaacttatta
     1261 tcttattgat gataagggct gggcatcaca agaagatcta gttcaatttg ataaccgcat
     1321 gttaaaagtc caggaaatgc tcttacaaaa atataataac ccaaattatt caatttttgt
     1381 aaagcaactc aacacacaaa caagtgctgg tattaatgct gataaaaaaa tgtatgctgc
     1441 aagtatctcg aagttagcac cactttatat tgttcaaaaa caattacaaa aaaagaaatt
     1501 agcagagaat aaaactttga cttatactaa agatgttaat catttttatg gagactatga
     1561 tccattggga agtggtaaaa ttagtaaaat agctgataat aaagattatc gtgttgaaga
     1621 cctactgaaa gctgtagcac aacaatcgga taatgtagca actaatattt taggttatta
     1681 tctatgtcat cagtatgata aagctttctg ctcagagata aaagctttat caggtatcga
     1741 ttgggatatg gagcagcgct tattaacttc tcgttcagct gcaaatatga tggaagctat
     1801 ttatcatcaa aaaggccaaa ttatttctta cctttcaaat accgaatttg atcaacaacg
     1861 tatcacaaaa aatattactg ttccagttgc acataaaatt ggtgatgctt atgattataa
     1921 acatgacgtt gctattgttt acggtaatac tccatttatt ttgtctattt ttacaaataa
     1981 atcaacctat gaagatatta cggctattgc agatgacgtt tatggtattt taaaatgatg
     2041 acctatcaag aaatttttaa tgaaataaaa aataaagcct actttaaaaa tcatagacat
     2101 gtgttaatag ccgtatctgg tggagtagat tcaatgaatt tattgcactt tttgtacctg
     2161 tttcaagaca aattgacaat tcgtattggt attgctcatg ttaatcacaa acaaagatca
     2221 gaatcagatt cagaagaagc ttatttaaag tgttgggcta aaaaacatga catacctatt
     2281 tatgtttcta attttgaagg aatattttca gaaaaagcag cacgtgattg gcgctatgct
     2341 ttttttaaga gtattatgtt aaaaaataac tattcagctt tagtaacagc tcaccattca
     2401 gatgatcaag cagaaactat tttgatgagg ctaattagag gaagccgatt gaggcacttg
     2461 tcgggtataa aaagcgtgca
//
LOCUS       DAUGFP010000083         2451 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.083, whole genome shotgun sequence.
ACCESSION   DAUGFP010000083 DAUGFP010000000
VERSION     DAUGFP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.083"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..499
                     /locus_tag="VH663_001577"
     CDS             <1..499
                     /locus_tag="VH663_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174750.1"
                     /translation="IDWENVSDDIEPLKIHRNFYESISEKTDSEVIEEIKNLEDINFT
                     DEKGNDLDDLTIIVPAQDEGFKRVFLGQREWYDIRIGKSKRNRIKYIAGYEVAPESGV
                     KYWAEVDKIVPSDNYVGYWKVIFKGEVKEYSEYKKLGNTWPPQNSRYVSKKALDEAKY
                     LSDVF"
     gene            519..1811
                     /gene="purB"
                     /locus_tag="VH663_001578"
     CDS             519..1811
                     /gene="purB"
                     /locus_tag="VH663_001578"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEQ8174751.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEQYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDIKVTSCLTQEEIDELFNPIYYTKRVDDIFKRLGI"
     gene            1941..>2451
                     /gene="rgg4"
                     /locus_tag="VH663_001579"
     CDS             1941..>2451
                     /gene="rgg4"
                     /locus_tag="VH663_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HEQ8174752.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
                     LILLELYLAY"
BASE COUNT          807 a          394 c          550 g          700 t
ORIGIN      
        1 aattgattgg gaaaacgttt ctgatgacat agagccctta aagatacatc gtaactttta
       61 tgaatcaatt agtgaaaaaa cagattctga agttatagaa gagatcaaaa atttagaaga
      121 tataaatttc actgatgaaa agggtaatga tttggatgac ttaaccatta ttgttcctgc
      181 gcaagacgaa ggatttaaaa gagtctttct aggtcaacga gaatggtatg atatacggat
      241 aggaaaatcg aaaaggaata ggattaaata tatcgcagga tatgaagttg caccagagag
      301 tggtgtaaag tactgggctg aagtggataa aatagttcct tcagataact atgttggtta
      361 ctggaaagta atttttaaag gagaggtgaa ggaatattca gaatataaaa aattaggaaa
      421 tacttggccc cctcaaaaca gtagatacgt atctaaaaag gctttagatg aagctaagta
      481 tttatcggat gttttttaat taaaggagaa aaaacaaaat gatttcacgt tattcacgcc
      541 ctgagatggc ggcaatttgg acagaggaaa ataaataccg tgcttggttg gaggtcgaga
      601 ttttggctga cgaggcatgg gcagagttgg gtgagattcc taaggaggat gtggctaaga
      661 ttcgtgagaa ggcggatttt gacattgacc gcattcttga aattgagcag gacacgcgtc
      721 acgatgtggt ggctttcacg cgtgcggttt ctgagacgct tggtgaggag cgcaagtggg
      781 tgcactacgg tttgacgtca actgacgtgg tggacactgc ctacggttac ctctacaagc
      841 aagctaacga cattatccgt cgcgatcttg agaatttcac caatatcgtg gcagacaagg
      901 cgcgtgagca caaaatgacc atcatgatgg gtcgtaccca cggtgttcac gccgagccaa
      961 cgactttcgg tcttaagttg gcgacttggt acagcgagat gaaacgtaat attgagcgtt
     1021 ttgaacatgc tgccgcaggt gtggaagctg gtaagatttc aggtgccgtt ggtaactttg
     1081 ccaacatccc accttttgtg gaacaatatg tctgtgacaa attaggcatt cgtccgcaag
     1141 aaatttcaac acaagttctt ccacgtgacc ttcacgcaga atattttgca gtgcttgcaa
     1201 gtattgcaac atctatcgaa cgtatggcaa cagagattcg aggtctgcaa aagtcagaac
     1261 aacgtgaagt tgaagaattc tttgccaaag gtcagaaagg tagctctgct atgcctcaca
     1321 aacgcaaccc aatcggttca gagaacatga cagggctagc gcgcgtgatt cgtggtcaca
     1381 tggtgacagc ttatgagaac gtgtcacttt ggcatgagcg tgacatttcg cactcatcag
     1441 ctgagcgtat catcacacct gacacaacta tcttgattga ctacatgctc aaccgctttg
     1501 gcaatatcgt taagaacttg actgtcttcc cagaaaatat gatgcgcaat atggaatcaa
     1561 cttttggttt gatttatagt caacgtgtta tgctcaaatt gattgaaaaa ggaatgacac
     1621 gagaagaagc ttatgactta gttcaaccta agacagctta ttcctgggac aatcaagtgg
     1681 atttcaaacc acttttagaa gaagacatca aagttacctc ttgtcttaca caagaagaaa
     1741 ttgatgaact atttaatccg atttattaca caaaacgtgt tgatgatatt tttaagcgtt
     1801 tagggattta atataaaaaa agaaggtgat tcccttcttt tttatttaaa aaacgaatat
     1861 tttctttaaa aatattaaaa aggttagttt ttaattagac gctgtgatat aataaagaaa
     1921 acagttgttg gagagaaacc atgttagaac attttggtgg aaaagtaaaa gtgttaagac
     1981 ttgaaaagag gattagtcgc gaggacttgt gtggggatga gtctgaactt tctgttcgtc
     2041 aattagcacg gatagaacta ggtcaatcca taccaagttt aagtaaggtt atttttattg
     2101 caaaagcctt aaacgttagt gtcggttact taactgatgg tgctgattta gaactaccta
     2161 agcgttacaa agaattaaaa taccttatct taaggacacc aacttacatg gatgatggaa
     2221 aattacaagt acgagaagag cagtttgatg aaatttttga ggattattat gataaattac
     2281 cagaggaaga gaaaataatc attgattgtt tacaggcaac tttagatact ttattgagtg
     2341 agaatactaa ctttggcatt gacttacttc aagaatattt taatcaaata aagactaagg
     2401 tacgctttag gcaaaatgat ttaatacttc tagaattata tttagcttat c
//
LOCUS       DAUGFP010000084         2355 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.084, whole genome shotgun sequence.
ACCESSION   DAUGFP010000084 DAUGFP010000000
VERSION     DAUGFP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.084"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..176
                     /locus_tag="VH663_001580"
     CDS             <1..176
                     /locus_tag="VH663_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ8174753.1"
                     /translation="EKKKIFDRFYRVDKARTRQTGGFGLGLALAQQIVMSLKGNITVK
                     DNDPKGSIFEVKL"
     gene            complement(301..549)
                     /locus_tag="VH663_001581"
     CDS             complement(301..549)
                     /locus_tag="VH663_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEQ8174754.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(603..1523)
                     /locus_tag="VH663_001582"
     CDS             complement(603..1523)
                     /locus_tag="VH663_001582"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEQ8174755.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            1758..>2355
                     /locus_tag="VH663_001583"
     CDS             1758..>2355
                     /locus_tag="VH663_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ8174756.1"
                     /translation="MKKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIFGNVEILRKDKGY"
BASE COUNT          790 a          333 c          436 g          796 t
ORIGIN      
        1 aagaaaagaa aaagattttt gatcgttttt atcgagttga caaagctaga acacggcaaa
       61 caggtggatt tggcttgggg ttggctttag ctcagcaaat cgtgatgtct ttaaaaggaa
      121 atattacagt aaaggataat gatcctaaag gtagtatttt tgaagtcaaa ttataagcta
      181 aaaagggcga ccaataaggt cagccctttt aaagttattt ttaactgtca acgctttatt
      241 gtattttttg agtttttacc gtataaaaaa actccagctg gagttttcat tggatattta
      301 ttagccaagt tttgcagcaa ggcgtgcttt atcgcggctt gctttgtttt tgtgaatcaa
      361 accttttgat tcagctttat cgatgcttga agaagcagcg cggaaaagct cttcagatgg
      421 gttagcttca aatgctttga tagcagtacg catagctgat ttttgtgctg agtttttttc
      481 gtttgcttta acgttaagtt cagcgcgttt aatagctgat ttaatatttg ccaatgtttt
      541 cacctccaaa ttaatctaac tacattatta tatagaaaat atatgcgttt ggcaaccctt
      601 tatcactttt tgaggtaaat ttcatcaatt ttatgatcag cacttttatg taaaattaat
      661 tcagcacggt tacgtgtagg ttcaatgaat ttttcaagat tttctaaatt aacggttttc
      721 caaacgttgc gcgcaaatgc tatagcctca gatcgaggta attgtgcata ttgggcataa
      781 taattatgag ggtcacgttt tgctaatttt aaaatgctta gaaaccgttc aatgtaccag
      841 gtttcgatgt gactgctgtc tgcatcaata taaatagaaa aatcaaagta atcactcata
      901 tataaacgat tgttttgttg attttgaaaa acattaatac cttcgacgat aaggaagtca
      961 gggttattaa atgattgttt ttgatttggg ataatatcgt aaatatcatg agaatagacg
     1021 ggtgcaaaag ccgtttgacc atttttgatg gaatctagaa aatctaagag aagttccatg
     1081 ttataagatt ctggaaatcc tttacgattt aacagtcctt gttcaataag aaactgatta
     1141 ggatataaaa aaccatcagt cgttaccagt tcgacttgac tattggggtg cgttcgtgat
     1201 aataaaagtt gtaaaagacg actagttgtt gatttaccaa cagctactga accagaaatt
     1261 ccaataataa aaggccttaa ttgaatgtct ttttttagaa aaaggctttt agaaaaagaa
     1321 agattttctt gcgcaatttt gtaaacctgg atcagattaa ttaatggaag gtagatatca
     1381 atgacatcgt taatactgat gttatcattt aagctagtaa tacttttaag ttcctcttgt
     1441 gtgaggagag cttttgcttt ttgatgtaat gttttccaag actctctaga aattttttca
     1501 aagttgataa attcatttga catggtagct cctaatcatt tgacagtgct tacattatac
     1561 cattttagtt aaaaaataaa taatttttcg ttagtattta cacgaaaatt aatacaatta
     1621 actcaaaaaa gccacacatt cctagtacaa ttatcctgct atattctaac aaatagttaa
     1681 agttcggttt ttatttgtaa tatagagaaa attgttaaga aacaagcgtc ttgtaaacca
     1741 tttcaaagta tgatagaatg aaaaagtacc ttaaaaagat aggaaaaatg atgacaaaga
     1801 tgtattatga tgaaaatcca gatagcctac acgatattca tgaagtaaaa gtagagctgc
     1861 ttaatcatcc ctttactttt ttaacggatt caggtgtctt ttcaaagaaa atggttgatt
     1921 tcggtagtca agtattatta gagacactaa atttcaagga gaacgaacgt gtactggact
     1981 taggttgcgg ctatggtcct cttgggatta gtctagccaa ggttcaaagg gtagacgcaa
     2041 ccttagtgga catcaataat cgtgctcttg atttagctag aaaaaatgca actaataacc
     2101 aagtggcagt aactattttt caatcaaata tttatgaaaa tattagtggc cattttgagc
     2161 atattattag taatcctcct attagagcag gaaagagagt agttcatagc attattgaaa
     2221 aaagcataga ttttttagtg gttaatggtg acttaacgat tgtgattcaa aaaaaacaag
     2281 gagctcctag cgctaaagca aaaatggcaa ctatttttgg taatgttgag atactaagga
     2341 aagataaagg ttatt
//
LOCUS       DAUGFP010000085         2311 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.085, whole genome shotgun sequence.
ACCESSION   DAUGFP010000085 DAUGFP010000000
VERSION     DAUGFP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.085"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..252)
                     /locus_tag="VH663_001584"
     CDS             complement(<1..252)
                     /locus_tag="VH663_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ8174757.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRIPVGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLA"
     gene            complement(230..1096)
                     /locus_tag="VH663_001585"
     CDS             complement(230..1096)
                     /locus_tag="VH663_001585"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEQ8174758.1"
                     /translation="MKRIIVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLSLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLDYLTKSFKK
                     LLEEKNVISKIS"
     gene            1186..>2311
                     /locus_tag="VH663_001586"
     CDS             1186..>2311
                     /locus_tag="VH663_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEQ8174759.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYYKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYSTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPYFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTS"
BASE COUNT          783 a          436 c          355 g          737 t
ORIGIN      
        1 agctaaggat tctaagatag ttatccaaac gactgaagca tagccgttca aaacatcaaa
       61 gatccctaaa ccaagactag caactagagc tcctttttta ctaccataga ttaagactgc
      121 aataacaact aatgcattgc caagatgaat aaactgcata ccgacaggga tacgcaacac
      181 ttgtactgaa ataaaaatta aagcagcata catactcaat tcaatataat tagctaattt
      241 ttgagatgac atttttttcc tccaacaact ttttaaacga tttagttaag taatctaaat
      301 gagattcata agcaacacct aagattaaat ctctttttaa tgtaagggta ttttgcaata
      361 ctttttcaag aaatgaaaca acaagatcta gaatttgtgt tagtggtagc tgataataga
      421 atcctgctgt tataatagct gatgctatat ctcctgtacc atagaaatgg taagggaatt
      481 gcttcccaaa atggaatgtt aattgatctg aatctgactg atagtaagca aaaccaatgc
      541 tgccctcctc aaaagagatt ccgctaatga caacctgttt aggtccaagc ttagctaatt
      601 ttctagccac ctgtctaata tcttcttcat cataattttt tgctaaataa gctgtttgcg
      661 ttaatagaca agcctcacta atattaggta aaacaagctc agctttttga gtaagtttta
      721 tcataatctc tatatgcttt tgactgaagc cttgataaag ttttccatta tctgccataa
      781 ttggatctaa aattagggaa agttgctttc ttgaaagcca ttctagtagc ttttgcccat
      841 ctcttgttgt cgtaaaataa cctataaaga tgccgtcaaa cttaattctt ttgttgtccc
      901 aatctgttaa aaaaacgtct aatatacttg atgtagtatg cttcacaggg ctctgtcctc
      961 cgccagtatg gcttgataaa aggcaagttg gtaataaaac ttgctctaac aaacaagaag
     1021 ccattaaagg aatactagca gacaaagcta ccttaccaac accaaccaga tcatttgcca
     1081 caataatccg tttcatagct attacctctt ttctataata actattgtaa gaaaaagagg
     1141 cataagatac aatagttata acacttaact gtatggggac aatttatgac aacaaaatat
     1201 caaacaatca tttccaatat cgaacaagat atccagaaac agcgactaaa aaaaggggac
     1261 aagctacctt caattagggt actaagtaag gtttatcatt gtagcaaaga tactgttcag
     1321 cgggctcttt tagaattaaa atatcgtcac ttaatttatg ctgttcctaa aagtggttat
     1381 tatgttttgg gtaacgtaag catgcccgat aatgttctta atctaagtct tgaagattat
     1441 aataatatgg cttatgaaga tttccgactc tgcttaaatg aggccttaag tgctaaagac
     1501 aaatacctct ttcattatta ttacaaaacc gagggactag aagaactaag ggaagctttg
     1561 cttctttact tagctgaaaa tagtgtttac agtaacaaag accaactatt aatcacctct
     1621 gggacgcaac aagcgcttta tattttatca caaatgcctt ttcctaatgc gggcaaaaca
     1681 attctccttg aaaagcctac ttatcatcgg atggaagcta ttgttgctca gttaggatta
     1741 ccctatcaaa ctatctctag acactttaat ggccttgatt tagaactctt agaatcttta
     1801 tttcaaactg gcgatattaa atttttttac acgatttcac gattttcaca tcctctggga
     1861 ctttcttata gcactaagga aaaggaagct attgttcgtc tagcacaacg ataccaggtt
     1921 tatattttag aagatgatta tttaggagat tttgtgaaac ttaaagagcc accaattcat
     1981 tattatgaca ctcatcaccg aatcatctac ctaaaatctt tttcaatgag tgtttttcct
     2041 gcactccgta tcggagctct agttttaccg tcaggtttaa aaccttattt tttaacccaa
     2101 aaatcactga ttgatttaga cacgaactta ctaatgcaaa aagcattagc tctttattta
     2161 gaaaatggga tgtttcaaaa aaatcttcgc tttattaagc gttatctaaa acagcgggaa
     2221 cgtcaattgg ctctcttttt aaaacaaaat tgccctgata ttcactatca actaacccct
     2281 actcatttag tgattgatta cacgacatca g
//
LOCUS       DAUGFP010000086         2297 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.086, whole genome shotgun sequence.
ACCESSION   DAUGFP010000086 DAUGFP010000000
VERSION     DAUGFP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.086"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(179..865)
                     /locus_tag="VH663_001587"
                     /pseudo
     CDS             complement(179..865)
                     /locus_tag="VH663_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1182..1398
                     /locus_tag="VH663_001588"
                     /pseudo
     CDS             1182..1398
                     /locus_tag="VH663_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
     gene            1411..1611
                     /locus_tag="VH663_001589"
     CDS             1411..1611
                     /locus_tag="VH663_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEQ8174760.1"
                     /translation="MTTMKELTISDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHP
                     VLTGGCVVGFTVGGAYLGYTAN"
     gene            1905..2099
                     /locus_tag="VH663_001590"
     CDS             1905..2099
                     /locus_tag="VH663_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEQ8174761.1"
                     /translation="MEVLKNGLAIINGGGKDGANIFLTGMVGAAQGVIVCAQTGVFIP
                     LQGYLLCGAAGAATNIIWPH"
BASE COUNT          752 a          324 c          458 g          763 t
ORIGIN      
        1 tttcccaaat ttttttgaag gataaagagg taagagaaaa tgcaagtaaa gtaattttaa
       61 ttgtgatatt tgtttatgtc ttactaagta ctattacaaa ttaataatat taaagggata
      121 tagattaatt gagactgtca ataatgttgt gtaaacacaa gagtagagtt tttgaatatt
      181 agtcaaataa gctatcaagt gtgtcaaaac atactccgaa tcctttatga atacgagctc
      241 cgaacttaat attatagtct tcgaaaatac ttacaagaca tcgttctaag gcttcttcat
      301 ttggaaaaac caccctcctt cttgctttgt cgcttgattt ctttattgag tgattcaatc
      361 aagtttgtag agtaaatact agaccaaatg taatgtggaa attgatagaa agttaggaga
      421 ttttccgttg tttcaagtga tgtcataacc tttttgtaac gaggcttcca ctcagaaaca
      481 agtggtgacc atacatgcgc tcaataatgt cactgatttc gcgtgtcgta acgccagtct
      541 gatagagttt gatcaccatc tcttccaagt ggtcatctcg tctagcataa gatggtaata
      601 aaactggtga aaactcgccg tttcgatctc ttgggatgat taaattgact aagccatact
      661 ttgtttcaaa ctgacgagaa taagtcccat tacggctatt acctgtgttg caaccttctt
      721 tttcataagg ttcataacca agaaaagctg atagttcaac ttgcaagagg tcattcatag
      781 ctatttctaa agaggagcga aaaaattcat caatatcttg tttttgtgct aggaagttaa
      841 gtaattctgt ggtaaactga gtcatgggaa taaatctctt tctagtaagt ttcgtaactc
      901 tactatacag gatttattcc tttttgtgtg tttacacaag atattttaca ctaccatcaa
      961 aaatcttaaa aggtatttat aaaagcatga atggtattat ttaaacttct taattaatta
     1021 actattgtca cagcgtaagt gtagagagga taaaaagaca tttcatgatg aaaaacagcc
     1081 ttttaagatt ttgaaaccat gatactgttc tttatgatat tctgtactag tcgaggttgc
     1141 aacgagacaa atttaagaat tatgaaaaga aattattcga tatggagata aagaaactgg
     1201 aaacatttca ccaaatgact atagaaaagc ttgccaaggt tgagggtggt aagaataatt
     1261 ggcaggcaaa tgtcagtggg gttatcgctg caggtagtgc tggagctgct ataggttttc
     1321 cagtttgcgg tgtagcctgg agctaaaaca gctattactc tttgggctgg tgtaacagga
     1381 gctacaggcg gattctagag gaaggaaagg atgacaacaa tgaaggaatt aacaattagt
     1441 gacatggcta gtataagtgg aggaaacgct ccaggagatg ctgtaattgg tggtcttgga
     1501 ggcttagctt ctggattgaa attttgtaaa ctgccacatc cagtattgac aggtggatgt
     1561 gttgtaggat ttacagttgg tggagcatat ttgggatata cagcaaatta aatgatctat
     1621 acgattttta ttattatgca tgcgtctata atgtttttaa tgattgttga tgccattcat
     1681 aaaaaaatgt tgataaatct ataccatatt tagtgttttt aattttcaat caattatccc
     1741 tttcggaaaa atcggtaaac atgttatcta tcggagcttt ggtagcgtca ttatttgttt
     1801 ttatcgataa aagaaaatag tgattatgcc aatgaattta gatatcactc ttagcttttt
     1861 aaaatacaaa agagtgaaat taaaatgaaa aagacttcaa aaatatggaa gtgctaaaaa
     1921 atggtctggc tatcataaat ggaggcggaa aagacggagc aaatatcttt ttgacaggga
     1981 tggtaggtgc tgcgcaggga gtgatagttt gtgctcagac aggcgtattt atccctttgc
     2041 aaggttatct tttgtgtgga gcagctggtg ctgctacaaa tattatctgg cctcactaaa
     2101 tatataaatg actatctatc actgagtata gtagtagagg agtggtatga aaactttaga
     2161 tattgttgtt tgctatacaa taggaatgat tttatcatta ggtcgtctta tttctcctaa
     2221 tcactctata tttaataaac ttaaaaagtg aaatgaatca tatttaaagt ttgtttttaa
     2281 ataatgggac tagttta
//
LOCUS       DAUGFP010000087         2209 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.087, whole genome shotgun sequence.
ACCESSION   DAUGFP010000087 DAUGFP010000000
VERSION     DAUGFP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.087"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1350
                     /gene="cls"
                     /locus_tag="VH663_001591"
     CDS             <1..1350
                     /gene="cls"
                     /locus_tag="VH663_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEQ8174762.1"
                     /translation="DAISRMTWLILIMIAPLLGSLFLIYTKLDWGYRGLKQRINHLVD
                     LSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIYNNTRVTYFPTGETFFDSL
                     KEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVEVRVLFDGMNELSTLSSDY
                     AKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEVSFTGGINLADEYINEVER
                     FGRWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYLSDHSLKLPSDGYVIPYGD
                     SPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHALRFASERGVDIRIIMPGV
                     PDKGVPYALAKTYYKALMTSGVKIYEYQPGFVHSKVFISDNTKAVVGTINLDYRSLYH
                     HFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLKQRPWYQKLIGLLVRTIAPLL"
     gene            1689..2027
                     /locus_tag="VH663_001592"
     CDS             1689..2027
                     /locus_tag="VH663_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ8174763.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT          707 a          338 c          420 g          744 t
ORIGIN      
        1 gatgctattt caagaatgac gtggttaatt ttgattatga ttgctccatt actagggtca
       61 ctgtttttaa tttatacaaa attggattgg ggttacagag ggctaaaaca aagaataaat
      121 catcttgtag atctgtctgc accttacctt agcgatgatg acgctatatt agaggtatta
      181 aaggatagca catcaaccac atatcattta gtacagtact tagaaagaag tcgtggcaac
      241 tttccgattt ataataatac aagggtaact tacttcccaa caggtgaaac tttttttgat
      301 agtttaaaag aacagttatt cttagctaaa aagtacattt ttcttgaatt ttttattatt
      361 gcagaaggcc aaatgtgggg agaaatcctt agtattctag aaaaaaaagt cagtgaaggt
      421 gtagaagtta gggtgttgtt tgatggcatg aacgaactat caacgctatc atcagattac
      481 gccaagagat tagaacaaat agggattaaa gctaaatcat ttttaccgat ttcacccttt
      541 atctctacct attacaatta tcgagatcac cgaaaaattg tcgttattga tggggaggta
      601 tcattcactg gaggtattaa tctagcagat gagtacatta atgaagtaga gcgttttggc
      661 cgctggaaag atgctggttt aatgcttgag ggtgaagcaa cagacagctt tttaattttg
      721 tttttacaaa tgtggtctat cacagaaaaa gaactgatta ttgatcctta tctttcagat
      781 cattctttaa aacttccttc agatggctat gttattccct acggtgattc cccgcttgat
      841 actgataaaa taggtaaaaa tgtttatata gacattttaa atcatgctaa agagtacgtt
      901 tatatcatga caccttacct tattttagat agcgagatgg aacacgcttt acgatttgca
      961 tcagaacgtg gagtagatat ccgaattatt atgccaggag tgccagataa gggggtacca
     1021 tatgccttag ctaaaaccta ttataaagct ttgatgactt ctggagtaaa aatttatgag
     1081 tatcaaccag ggttcgttca ttcaaaggta tttatctctg ataatacaaa agcagttgtc
     1141 ggaacaatta acttagacta tcgtagcctt tatcatcatt ttgaatgcgc gacttattta
     1201 tatcgtgtgt cagttattgc tgatattgtg aatgacttta atgaagctca aaagcaatca
     1261 ctattgatga catcagatca tttgaagcaa cgtccttggt atcaaaaatt gataggatta
     1321 ttagtacgaa caattgcccc tctcttgtaa ttttcgtaaa atatgttata atatctgtat
     1381 gcgatgagtc gattgtgact gcaaagtcac ttttgcgcag gggaggtcat taaggcggga
     1441 agccgacttt gataatctgt gtgaaccatt ttatcacaat cttatttatt aagaatgccc
     1501 cttggattga gatattctca atcctttttt aattttatta tcttagttaa aattcagaat
     1561 gtatcagtga ttactgtgtt tcaaaataag aaagtagttt ttcgggtatg agtgctatga
     1621 cttgacaaat gctaataaca tttgcataat gtaagtagag atgaaaactt atgaaaagag
     1681 atgatcttat gcaaattatt aagcgtgatg gccacgttgc tgattttgat ccagataaaa
     1741 tttatcaagc aattattaaa gcagcaagga cggtctatgt tattgatgac acgtggcgac
     1801 aaaacttaac acatgtcact aaaaaagtag ttattgattt agaagaagca catgtagaac
     1861 gtcctactat caatatggtc caatcccttg tggagaatcg tttgatggat gctggatata
     1921 ttaccattgc agaacattac atttcttatc gtcttcagcg agatttagag cgcaatggct
     1981 atggtgataa gattgtggtt cacttgcgtt ttgaacaaat aaaataaaaa cgcttctaat
     2041 ggcattttag tgtctttgag gggataaggt ttaaatacct aacagataaa aatctgttag
     2101 gtattttttt gctactatta ggtgtattct atctcacata gaacttaagc attcaagctt
     2161 taatttaact ttctgtttat tttttgctac attttgggaa actcccctc
//
LOCUS       DAUGFP010000088         2126 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.088, whole genome shotgun sequence.
ACCESSION   DAUGFP010000088 DAUGFP010000000
VERSION     DAUGFP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.088"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            124..2115
                     /gene="uvrB"
                     /locus_tag="VH663_001593"
     CDS             124..2115
                     /gene="uvrB"
                     /locus_tag="VH663_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEQ8174764.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
BASE COUNT          705 a          356 c          466 g          599 t
ORIGIN      
        1 tgttatttat atgtcataaa acataacatg taaatgacat gttttttacg cttattggag
       61 gtgattctgg cttatctatg ttaaaatagt ctataagaga gtaagactat tagagggtat
      121 aaaatgattg ataaacgtga tgataaacca tttaaactaa aatcaaagta taaaccgtct
      181 ggtgatcagc cacaagctat tgaaagccta gttgataata tcgagggcgg agaaaaagct
      241 cagattctcc taggtgctac aggaacggga aaaacataca ccatgagcca agtaattagt
      301 aaagtaaata aacctacctt agtgatagct cacaataaaa ccttagcagg acaattatat
      361 ggtgaattta aagaattttt tccagacaat gctgtggaat attttgtttc ttattatgat
      421 tactaccaac ctgaagcata tgtcccatca agcgacacct atatcgaaaa agatagttca
      481 gtcaatgatg aaatcgataa acttcgccat tcagccacct cttctttact tgagcgtaac
      541 gatgtcatag tggtagcttc tgtatcttgt atttatggac taggttcgcc aaaagagtac
      601 gcagattcag cagtcagcct acgtccaggt caggagatat cacgtgacac tttgctaaat
      661 caattagtag atattcaatt tgagcgtaat gatattgatt ttcaacgcgg ctgttttcgt
      721 gtacgtggcg atgttgttga ggtctttcca gcatccaggg atgaacatgc ttttcgggtg
      781 gaatttttcg gggatgagat tgaccgtata tgtgaaatcg aaagtttaac tgggaaaact
      841 attggagaag ttgaccattt ggttttattt ccagctactc actttgtaac aaatgatgag
      901 catatggagc aatcaatagc caaaattcaa gcagagttag cagagcagtt acagttattt
      961 gaatctgaag gtaaactact agaagcacag cgattacgcc aaagaacgga atatgatatt
     1021 gaaatgttac gtgaaatggg ctatactagc ggagtcgaaa actattcccg tcatatggat
     1081 ggcaggtcac caggagaacc tccttatacc ttgttagatt tcttcccaga agatttttta
     1141 attatgattg atgaaagtca catgacaatg ggacaaatca aaggcatgta taatggtgac
     1201 caagccagaa aacaaatgct agttgactat ggttttcgat tgccatcagc tcttgataat
     1261 cgtcccttaa gacgcgagga atttgaaagt catgtgcacc aaattgtcta tgtttcagca
     1321 acgcctggtg aatacgaaat gtcacagact aatacaatta ttgagcaaat tattcgtccg
     1381 actgggctac ttgatcctga aattgatgta aggccaagta tggggcaaat ggatgatttg
     1441 ttgggagaaa ttaaccagcg cgtagcgcgc gatgaacgga cgttcatcac aaccttaact
     1501 aaaaaaatgg cagaagattt gactgattat ctaaaagaga tgggtgtcaa ggtcaagtat
     1561 atgcacagtg atattaaaac cttagagcgg acagaaatta ttcgtgattt acgacttggt
     1621 gtttttgatg tcttgattgg aatcaatctg ctccgtgaag ggattgatgt gccagaagtt
     1681 agtcttgtag ccattttgga tgctgataaa gagggtttcc tccgtaatga acgcggcttg
     1741 attcagacca tcggtagagc agctcgaaac gtagatggtc atgtcattat gtatgcggac
     1801 aagatgacag attctatgca gcgggctatt gacgaaacgg ctcgtcgtcg tgagattcaa
     1861 atagcatata acaaagcgca cggcatcgtt ccacaaacta taaaaaaaga catcagaggc
     1921 cttattagta taagtaaaac aagccacaat gatatatcga aagaagagat ggattatgaa
     1981 agtatgtcaa gaggagaacg aaaagaagcg atcaatgctc tgcaaaaaca aatgcaagag
     2041 gcagctgaat tacttgattt cgaattggca gctcaaatgc gagacttgat tttagaactt
     2101 aaattaatgg actaggtttt gaataa
//
LOCUS       DAUGFP010000089         1821 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.089, whole genome shotgun sequence.
ACCESSION   DAUGFP010000089 DAUGFP010000000
VERSION     DAUGFP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.089"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..580
                     /locus_tag="VH663_001594"
     CDS             <1..580
                     /locus_tag="VH663_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEQ8174765.1"
                     /translation="LINPSESQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNL
                     TSYARLLSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDY
                     FIWSNGTNEKKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEV
                     KAIQNDLKNIQYDVTIGKGYLLKHKTFFKISR"
     gene            759..1454
                     /locus_tag="VH663_001595"
     CDS             759..1454
                     /locus_tag="VH663_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ8174766.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            1456..1797
                     /locus_tag="VH663_001596"
     CDS             1456..1797
                     /locus_tag="VH663_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEQ8174767.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFILALLI
                     LLFTWLPNILNKMSHFLGIHSPTNMLFFLGFCLSLAIIFSLTNNISLQNDKVKRLTQE
                     VALMKKEKTND"
BASE COUNT          619 a          271 c          317 g          614 t
ORIGIN      
        1 tttaattaat cctagtgaaa gccaattttt ctcagttatt acaatgcaaa atcatattcc
       61 ttggtcatcc gattatcctg aagaaattgt tgctgaagga aaaaatttca cggaagaaga
      121 aaatcacaac ctaacaagtt atgctcggtt attatcgttt actgataagg aaacaagagc
      181 atttttagaa aaattaacac aaattaacaa gcctatcaca gtggtgtttt acggagatca
      241 tttacccggt ttatatcctg atagtgcttt taacaagcat attgaaaata aataccttac
      301 tgattatttt atttggagta atggtactaa cgagaaaaaa aatcatccgc ttatcaactc
      361 aagtgatttt actgcagctt tatttgagca tactgattca aaagtatcac cttactatgc
      421 tttgttaaca gaggtactga ataaagctag tgtcgataaa tcaccagata gtcctgaagt
      481 taaagctatt cagaatgatt taaaaaatat ccaatacgat gtgactatag gaaaaggtta
      541 ccttttgaaa cacaaaactt tttttaagat atcacgttaa gaaaaatgct aggctgtccg
      601 agtatagacg agcatcaagg tttggtaatt tgctcaaatg attgtcaaca gagtgtctag
      661 gaccaggttt aaaagattat aattcttaag gtcttattcg tttataaaaa atgaatactc
      721 ttttaaaatc ttaattgaaa tgaataggag aagttttcgt gaaaaagtta atcattattc
      781 ctgcttacaa tgaaagcagt aatattgtca atactatacg tactattgaa tcagatgccc
      841 cggattttga ctatatcatt attgatgatt gctcaacgga taatacgtta gcaatatgtc
      901 aaaaacaggg gttcaatgtt atttctttgc ccattaacct gggaattggc ggtgcggtgc
      961 aaactggcta tcgttatgca caaagatgtg gatatgacgt tgcagttcaa gtagatggag
     1021 atggtcagca caatccatgc tatttggaaa aaatggttga ggtattagtt caatcttcag
     1081 taaatatggt aattggatca cgatttatca caaaagaagg gtttcagtca tcatttgctc
     1141 gacgtatcgg tataaagtat tttacttggc ttattgcgct actaacagga aaaaaaataa
     1201 cagatgcaac gtcaggtcta aggttaattg accgttcatt gattgaacgt tttgctaatc
     1261 attatccaga tgattatcct gaacctgaaa cagttgttga tgtattggtt agtcatttta
     1321 aagtgaaaga aattcctgtt gtaatgaatg agcgacaagg cggtgtgtca tctatctcgt
     1381 tgacgaaatc ggtctattat atgattaaag tgactttagc tattttagtt gttaggttga
     1441 aaggaaatcg ttaaaatgac ggtaacagcc agtttattta taagttttat tgtattaacc
     1501 tttgttttct ttttgattaa cttaatcaaa aaagacaaac tagcgattaa gtactcttta
     1561 ttgtggttta ttttagcttt attaatttta ctatttacgt ggttacctaa catattaaat
     1621 aaaatgtctc acttcctggg tattcattca ccaaccaata tgttattctt tttaggtttt
     1681 tgtttgagtt tagctattat tttttcactg actaataata tttcacttca aaatgataaa
     1741 gtaaaacgtc tgacccaaga agtggccctt atgaaaaaag agaaaaccaa tgattaatcc
     1801 ttcaaaacaa aaccaaacga t
//
LOCUS       DAUGFP010000090         1681 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.090, whole genome shotgun sequence.
ACCESSION   DAUGFP010000090 DAUGFP010000000
VERSION     DAUGFP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.090"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            73..714
                     /locus_tag="VH663_001597"
     CDS             73..714
                     /locus_tag="VH663_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEQ8174768.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGQVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWKSIQPNEEVDKVFIVPLRQLLVTDPVYYRLEATPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWRMTAQFTKCLTDILNDESIAKKNPLDIEKSKGR"
     gene            complement(761..1603)
                     /locus_tag="VH663_001598"
     CDS             complement(761..1603)
                     /locus_tag="VH663_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEQ8174769.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERVISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVIVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKVDWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMTVVLFALGIWLGLWMSQLE
                     EKYK"
BASE COUNT          597 a          302 c          332 g          450 t
ORIGIN      
        1 ataagttgac caaaaagcta ggattggtta tgatgaagat aagattaacg gaaagagaaa
       61 ttagggaagg ctatgaagga cttattaaaa cagtatcagg caaaaccact aggagaggag
      121 aagcggtacg ccgtattttt acctcttatt cttgtcaatg atgactggca tgtcttatat
      181 gaagtcagaa gtcagcatat ttcacaaccg ggggaagttt ctttcccagg gggacaagta
      241 gaaaatcagg agacacttca agaagctgct atccgagaaa cagttgagga attgactgtt
      301 gatgcgtccc agatccagct atggggtgaa attgattatt tagtacagtc ttctagaact
      361 attcattgtt tcgttggtca attagtcgtt gatgactgga aaagcataca gcctaacgaa
      421 gaagttgaca aggtctttat agtaccttta cgtcagctac ttgtcactga tcctgtctat
      481 tatcgtttag aagccactcc tattgagaca acggattttc cttttgaccg tattcgtaat
      541 gggaaatact accaatttag tcaagagtac cgtagtattc cgttttatga aaatctagaa
      601 gaaaccatat ggagaatgac tgcccagttt actaagtgtt taaccgatat tctaaatgat
      661 gaatcaattg caaagaaaaa ccccttagat attgaaaaat ctaaggggag atagagcact
      721 agtttgttgg cgtcaacttt ttgtcaacaa cctgtaaagg ttatttgtat ttttcttcta
      781 attgactcat ccaaagtccg agccaaatac caagagcaaa taacacaacg gtcataagcc
      841 aggttaagat acctgcatga ctgtaggaaa tggattcggg actactacta tttggaataa
      901 gaattaatag cgtgctggat aatacaatac caatgataaa gtggtaaact tttgaatgat
      961 ggtgctgtaa agcataatcc atacttttag aaaaagccaa gatggctagg actcctccga
     1021 tagcaatagg taagaaagtc cctaataaat ccaaagattt gaatcctatt aacataggac
     1081 catagagtcc taagataagt aagagatttg acgggcttag tccagggacc agtactccta
     1141 aagcaattaa ggcacctgct aaaataaagg ttaaaaagtt agctggtaag gtaccgatta
     1201 agtcatttaa gaagtaaaga cctagtcctg aaataacaaa ggtaaggact agccatagcc
     1261 agtcaacttt gtctctttga gactgcttgg ttgactcctt aatcagacta ggaatagtgc
     1321 cgacaatagc gccagcaaat ccccataaga cgataacttg atagtgctta agcaaaaact
     1381 caacggggaa agaaaagaga gcaataccta aaataccacc aattcctact ggtaagaaaa
     1441 ataagacatt ttcaataaag ttatccctca tatgagctaa aaaggaaatc actcgctcat
     1501 agattcctaa aatggctgcg agaacaccac cggatacacc aggtaaaata aaacctagtg
     1561 caataatcat gcctttaaaa actcttgaaa taaatgaaac cattgttgcc tcctgaaaag
     1621 aatgatttta ttgtatcata aatttaaaga attgctgaca caaagctgca tatccctact
     1681 a
//
LOCUS       DAUGFP010000091         1636 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.091, whole genome shotgun sequence.
ACCESSION   DAUGFP010000091 DAUGFP010000000
VERSION     DAUGFP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.091"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(199..948)
                     /locus_tag="VH663_001599"
     CDS             complement(199..948)
                     /locus_tag="VH663_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HEQ8174770.1"
                     /translation="MVQIFLSNLLKIKNTSISKLILIFPIICVMLSLLFSALGGESIL
                     RLTAETTVNQWGVIWINVIIAVNSGLLNKLELDSNKYSVFLSRDVDLKKVEYARVLLI
                     ALINLIISMILSLMLIVISFVLPTPSLISIGRILLTILLIWLTTLWQIPFILWLSRKI
                     NVYFAMIINIISPLIIGTSFSLLNKWYLFPYDWSLKLLEPMTRMRINSIPFGAEFVPD
                     YSQIFISLFLGIAFFILLTNLFAISFKKQVK"
     gene            complement(954..1634)
                     /locus_tag="VH663_001600"
     CDS             complement(954..1634)
                     /locus_tag="VH663_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ8174771.1"
                     /translation="MQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIMKIL
                     TGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVSEST
                     ILPLLNKVGLGDIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIGIQE
                     LREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIVLEKEYDGSENLEELFNN
                     QILFEKRR"
BASE COUNT          601 a          280 c          211 g          544 t
ORIGIN      
        1 caatacttgc aacaaaagga agaagtacac ctactatttg aaagtaggaa aataatacag
       61 tcctgctttg taaacctcca cgttgactcc aatagaaaaa tagaagtaag ggaaataata
      121 gtgcagctaa aatatgtatt agtaaatatg aagtattttt gatttttaaa aaattagaac
      181 ataaatatga tttcatgact atttcacctg ttttttgaaa gagatagcaa atagattggt
      241 cagtaagatg aaaaaagcaa ttcctaggaa caatgatata aaaatctgtg agtagtctgg
      301 aacaaactcc gctccaaaag gtatactatt tattctcatt cttgtcattg gctcaagcaa
      361 cttcaacgac caatcataag ggaacaaata ccatttattt aagagagaaa aacttgtacc
      421 aataattagt ggagatataa tattaataat catagcaaaa tacacattaa tttttcttga
      481 tagccataaa ataaacggga tttgccatag tgtagtcaac caaattaaca agatagttaa
      541 tagtatccta cctatactaa taagacttgg agtaggtagg acaaaactaa ttacaataag
      601 cattaagctt aaaatcatac taattattag atttataaga gctatcaata gaactctggc
      661 atattctacc ttttttaaat caacatccct tgataaaaaa acagaatact tattagaatc
      721 taattctaat ttatttaaca aaccagaatt tacagcaata ataacattaa tccaaataac
      781 tccccactga tttactgttg tttccgcagt taaacgtagg atactttcac cacctaatgc
      841 tgaaaacaat aaagataaca tcacacaaat aatagggaaa ataagaatca acttgctaat
      901 actcgtattt tttattttta ataaatttga taaaaaaatt tgtaccatag cccctatctc
      961 ctcttttcaa ataaaatttg attattgaat aactcttcaa gattctcaga gccgtcatat
     1021 tctttttcca gaacaatctt tccttcatag ataaatccaa taaaatcagc tagatgttca
     1081 acttctgaca gtatatggct tgaaatcata attgtcattc cttctgattt aaatgactct
     1141 ataatttccc ttaattcttg aattccaatt gggtccaagc cattagtagg ttcgtctaat
     1201 ataagtagtt taggtgaatt tattaaagag atagctatac ctaaccgttg cttcattcca
     1261 agagaaaatt gttttactgg tctcttgtca atatctccta gaccaacttt atttaataat
     1321 ggaagtatag tgctttctga aacaccaagc attgttgtaa ctaccttcat attatcgtaa
     1381 gcactcaaat ttttataaag tggtggttct tcaataatac tcccgatttt tcgcagatcc
     1441 cgacgtgacc attctctacc ttcaaaaata atagaaccac ttgtcttact aactaagcct
     1501 gtcaaaattt tcataatagt tgattttcca gcaccatttg gaccgattaa agcatacact
     1561 tttcccttag gaatattcat attgacatta ttcaatatgg ttctcttacc ataagatttt
     1621 tttagatttt gtattt
//
LOCUS       DAUGFP010000092         1411 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.092, whole genome shotgun sequence.
ACCESSION   DAUGFP010000092 DAUGFP010000000
VERSION     DAUGFP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.092"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(161..1282)
                     /gene="mnmA"
                     /locus_tag="VH663_001601"
     CDS             complement(161..1282)
                     /gene="mnmA"
                     /locus_tag="VH663_001601"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEQ8174772.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDEDGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVAVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT          439 a          305 c          232 g          435 t
ORIGIN      
        1 aacttttacg aactattttt aaaaataatg tgattatatt taaaatatac agatatcata
       61 aattaatcca ttaacgcatg attggttttt ataaataata tcatatgatg tcaaaggaat
      121 tgttgaatgt ccctttcctt ttatcttaat aaaagattca ttaaatgtac tgacaaggct
      181 gcccattttt ataagccatg tcaatcatac caccaccaag gcattctttt ccgtcataga
      241 aaacaacagc ttgaccagga gtaatcgcgc gctgtggctc tgcaaaaaca acttctgcct
      301 tatctcctcg aacatgcacc gccacgtgtg aatcgggttg acgatagcga aatttagcag
      361 tacattcaaa tgtgaactct tctggcatct cacgtgtaaa atggatgact gaggcgtcaa
      421 gactatttga cattagggct tcatggtaga aaccttgacc aacgtataaa atattttgcg
      481 acaaatcttt accgacaaca aaccaaggtt gattatcccc accatgctga ccaccaatgc
      541 ccagaccacc acgctgacca atagtataat acataagacc ggcatgttct cccatatcgc
      601 gaccatcaat agtcatcatg cgaccttttt gtgccggtaa gtactggctc aaaaactgtt
      661 tgaaattctt ttcaccaata aaacaaatac ctgttgaatc tttcttttta gccgttgcaa
      721 gccctgctcg ctcagcgatt tctctaactt ctgacttttg caaatgacca agggggaata
      781 aagtcttttg tagttgttcc tgtgatagtt gacttaaaaa gtaggtttgg tctttgccat
      841 tatcagctcc acgtaacatg tgaaccgtac catcttcatc acgcttgact tgagcatagt
      901 gtcccgttgc tacataatct gcacctaaag tcatggcata gtcaaggaaa gctttaaatt
      961 ttatttcctt attacacatg acatcgggat ttggagtacg acctgctcgg tattcggcca
     1021 agaaatattc aaatactctg tcccaatatt ctttttcaaa attgactgaa tagtaaggaa
     1081 taccgatttg atcagcaacg gctgctacat ctttgtaatc ttcagtagct gtgcaaacac
     1141 caaattcatc tgtatcgtcc cagtttttca tgaagacacc aataacatca taaccttgct
     1201 cctttaaaag tagagctgtt acagatgaat caacgccacc actcatacca acgacaacac
     1261 gtatctttga gttatctgtc ataaaaaatt ctcccatcaa taataagata gaaaaagata
     1321 atcatcatat tatgattctt tttcttatca gttaacgtac gattgaaggt cgtcgttttc
     1381 aaaagtcgat ttgaaggtcg attttgaagc g
//
LOCUS       DAUGFP010000093         1363 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.093, whole genome shotgun sequence.
ACCESSION   DAUGFP010000093 DAUGFP010000000
VERSION     DAUGFP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.093"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            54..812
                     /gene="mf2"
                     /locus_tag="VH663_001602"
     CDS             54..812
                     /gene="mf2"
                     /locus_tag="VH663_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEQ8174773.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAARVRTYPNVSHANTH
                     YKNTVSSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVAMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINLDYLNGTATPKN"
     gene            923..>1363
                     /gene="speC"
                     /locus_tag="VH663_001603"
     CDS             923..>1363
                     /gene="speC"
                     /locus_tag="VH663_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeC"
                     /protein_id="HEQ8174774.1"
                     /translation="MKKINIIKIVFIITVILISTISPIIKSDSKKDISNVKSDLLYAY
                     TITPYDYKNCRVNFSTTHTLNIDTQKYRGKDYYISSEMSYEASQKFKRDDHVDVFGLF
                     YILNSHTGEYIYGGITPAQNNKVNHKLLGNLFISGESQQNLNNKI"
BASE COUNT          490 a          229 c          215 g          429 t
ORIGIN      
        1 tgcaatattt gatccactct aaaagctaaa aatggaaagg tttaggtgtt atcatgaaat
       61 tatctaaaca aaaggcaagt ttgcttaccg ctgttttact gttactttct ctttctataa
      121 cgacaataac tgttgacgca gctagggtac gaacttatcc gaatgtatcc catgcaaaca
      181 cacattataa aaatactgtt tctagtaagc tcttaccttt tactgctaat tatcagttac
      241 aacttggtga gttggataac cttaaccgcg ctaccttttc acatatccaa ctgcaagata
      301 gacacgaaac taaagatgta cgcactaaaa taaattatga ccctgtggga tggcacaact
      361 atcaatttcc atacggagat ggctcaaaat cttcatgggt aatgaatcgt gggcatctag
      421 tcggatatca attttgtgga ttaaacgatg aaccaagaaa tttagtggca atgacagcct
      481 ggctaaacac aggtgcttat tcaggagcaa atgacagcaa tcctgaaggg atgttatact
      541 atgaaaaccg tttagattct tggcttgcac ttcaccctga cttttggcta gattataaag
      601 tcactcctat atatagtggc aatgaagtag tgcctcgaca aattgaatta cagtatgttg
      661 gaattgattc atctggtgag ttgcttacta taaggctaaa tagtaataaa gaaagtattg
      721 atgaaaatgg tgttactaca gtaatcttag aaaactctgc tccaaatatc aaccttgact
      781 atttaaatgg aactgccact cctaaaaact aaaatataaa tacatttata aaatttctaa
      841 ataaacagaa atctgatttt taactactta ctgctatttc atgtattctc gtacgagtaa
      901 tacatttaat taaggagaaa aaatgaaaaa gattaacatc atcaaaatag ttttcataat
      961 tacagtcata ctgatttcta ctatttcacc tatcatcaaa agtgactcta agaaagacat
     1021 ttcgaatgtt aaaagtgatt tactttatgc atacactata actccttatg attataaaaa
     1081 ttgcagggta aatttttcaa cgacacacac attaaacatt gatactcaaa aatatagagg
     1141 gaaagactat tatattagtt ccgaaatgtc ttatgaggcc tctcaaaaat ttaaacgaga
     1201 tgatcatgta gatgtttttg gattatttta tattcttaat tctcacaccg gtgagtacat
     1261 ctatggagga attacgcctg ctcaaaataa taaagtaaat cataaattat tgggaaatct
     1321 atttatttcg ggagaatctc aacagaactt aaataacaaa att
//
LOCUS       DAUGFP010000094         1324 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.094, whole genome shotgun sequence.
ACCESSION   DAUGFP010000094 DAUGFP010000000
VERSION     DAUGFP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.094"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            93..827
                     /locus_tag="VH663_001604"
     CDS             93..827
                     /locus_tag="VH663_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEQ8174775.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSEFFIEDNDVLCIA
                     SKVVSIAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIKGLDYKWSENTSIKEALNE"
     gene            820..>1324
                     /locus_tag="VH663_001605"
     CDS             820..>1324
                     /locus_tag="VH663_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEQ8174776.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKIFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKE"
BASE COUNT          497 a          161 c          273 g          393 t
ORIGIN      
        1 tttagaagta catgaaaaag aaatagacaa aaaggcagaa gtagtgagga agtcttatct
       61 gacactaaat gagtatatta atggaggcta aaatgaattt gagtatattt ggtttaaaga
      121 atattcctta tttgaaagaa ggggatagta tagaaaagct tattgaagaa agcattaaaa
      181 cttcagaatt ttttatcgaa gacaatgatg tgctttgcat agcttctaaa gttgtatcta
      241 tcgcagaagg ccaggtgatg tctttaaacg agatacaagt gagtgatgta gcaaaagaaa
      301 tccacaggaa tattcctcgt aaagatccta gaataattga aattatgcta aatttagtca
      361 atagagatct gtcacgttta gatatcaaaa aaaattacat tggttgtcgc ttggaaaatg
      421 gtttaaagtt gacaagtggt ggaatcgata gaaaatcagt agatgaagta tttcttttac
      481 ctaataatcc ggatgcttct gcaaaaagaa tttcggaata tcttaaaaaa tcacttggaa
      541 aaaatgttgc agttgttata acagatagtg atggtagaga ggataaaaga ggtgctactc
      601 aagtagcaat tggtatttat ggtattcatc ctttgagaaa aacagaagta atagattcac
      661 aaggagagac aataaaattc caagaagaga ctttgtgtga tatgattgca gcttgtgctg
      721 gcttagtaat ggggcaacgt ggaactggaa ttccagctgt cctcattaag ggcttggatt
      781 ataaatggtc tgaaaatact agtattaagg aagcgttaaa tgagtagaaa tcaggactgt
      841 aagaatattc taatcattgg ttttggaaaa attgggaaaa ttaaagcgta caagtggcta
      901 tcacgcggat atgaggtata tgtcaaagat ttgaaattct ccagcatgaa ataccttaat
      961 acaaatagtg atgtgttgga tgatttatca cgacaatatt ttactattga gatttgtact
     1021 ccgacaaatc accaccttac gctattgaaa accgttataa caaggtatgt ctattcttat
     1081 attagcattg aaaaaccact gtgtaacagg ttagaagatt tagaagaaat gaagtcgcta
     1141 gtagaaagtc acccacatct agcaaaaaaa atttttgcca gtgaacaata cttcttttcg
     1201 aaaattttgg aaaaactgtt acaagtagat tgtttctctc tagataaaat caaggaaata
     1261 acagttaatt tttctaaaga taggattgag gataacgaga atgggagatt tttagataaa
     1321 gaga
//
LOCUS       DAUGFP010000095         1310 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.095, whole genome shotgun sequence.
ACCESSION   DAUGFP010000095 DAUGFP010000000
VERSION     DAUGFP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.095"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(351..1124)
                     /gene="cfa"
                     /locus_tag="VH663_001606"
     CDS             complement(351..1124)
                     /gene="cfa"
                     /locus_tag="VH663_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEQ8174777.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTVLMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATINKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKV
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKPFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQNALETALKS"
BASE COUNT          425 a          220 c          204 g          461 t
ORIGIN      
        1 acataactat acttcatgat attcctaaag cgattcctag tcccaaaatg ataagtgaaa
       61 tagttaaaaa aatccctaca atttgttttt aaacagatca tagggatttt tttaagaata
      121 cacgttttca tattcaatct tattagttta ggaggttaaa caagattcaa tagtgacggt
      181 tgaacaatac cttaaattgg cacatcatgt taaaggcaaa aagggctaca ggtgactcat
      241 tgtttatgag aacatcatct caaactctac aatggtaaga tatttaaaat ctttacctat
      301 tctatatcct aatataatca tctacctata caacacatta cttatacact ctatgactta
      361 agagctgttt ccaaagcatt ttgcacagca ataacttcat cgtcaacttg ttgaacagtg
      421 gttgtcggat ttagttggac accaaccgca tgtgtgatag ctttgttaag tctattgtaa
      481 acgtcaaatg gtttgatccc taaaaccttt ttatctcttt ctaaacgagt gttccaaata
      541 gctttattta gtttagcttt agtatagata gtagcacggt ctgttggctg taaatcagga
      601 taattgataa ctttttcctc aagtgcttta atttcttgta cttgaacttt gatagcgtca
      661 acagatgcaa atggatctat aatacgaatg actaatttag taatagcaaa ccccatatca
      721 gtatgcgctt tttctacttt atgagttaat tgggttgtgg aaaatacgat tgctttgata
      781 gcatctgata atgcttctac tcttgcccca attgaactaa aatcatagat cgtttcagta
      841 ttgaagcgta tagaactctt catgtgatca acagcatcga tagctttgtt aatagtagcc
      901 tcaatatcag tacctttagc ttcatgttta atagcttcta attgttgatg cccctcatca
      961 agcttttgca ctagagcatc tctttgaaga ttcgcttggt tgttattcat taaaacagta
     1021 tcgggatttg aagcatcatc agctaaaact gagtgcgtca tagttgaaaa taaagctaat
     1081 ccagcaacaa ctgttgaaag acagagaagg tgtttgggtt tcatattatt cctcctgaat
     1141 ataataaacc tatttaaata gttaagtgaa gttaaattaa tttataagcg cttataacgt
     1201 tatcatacta cttgttttta ctaaggtcaa tatttcttta atttccagaa aataagagga
     1261 tttcacgaaa ataatcattt tttaatgtca ttaaatgcct agtattactt
//
LOCUS       DAUGFP010000096         1275 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.096, whole genome shotgun sequence.
ACCESSION   DAUGFP010000096 DAUGFP010000000
VERSION     DAUGFP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.096"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            246..>1275
                     /locus_tag="VH663_001607"
     CDS             246..>1275
                     /locus_tag="VH663_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ8174778.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQVDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQL"
BASE COUNT          442 a          190 c          211 g          432 t
ORIGIN      
        1 ataagagcta tttgactatt ccaaagtgag acgttatttg atataatata atctgtattt
       61 ttaagtataa agtgtggttg aaacttttta gaggtattac tggccttatt ttgcagtaaa
      121 tcataggagc aagtcaattg cttctttttt tgtttaaccg taaggaattt aaaaagttat
      181 cactcaccta gcctgaataa aaatggtata atcgtaagga tcacactatt tattaaggaa
      241 acattatgaa aattgttcag ggagtacaac ttcatctgat taagaccaaa cagtttaaaa
      301 ccaatcatat tacctttcgt ttttcaggtg atttgaacca aaaaacagta gcaaaaagag
      361 tcttagtggc acagatgtta gcaacagcta atgagtgtta tccaactgtt aggcagttta
      421 gagaaaaatt agcacgactt tatggagcta gcttgtcaac taacgtgtta acaaaagggt
      481 tagttcatat tgtggatatc gatattactt ttattcaaga tagatatgct tgcaatgggg
      541 aaaaaatact agatgaaatg attcagtttt taaaagatat cttgttctca cccctattat
      601 ccatcgccca gtatcagcct aaagtttttg agactgaaaa aaataatttg attaactaca
      661 tcgagtcaga tagagaggat tctttttact acagctctct taaggtaaaa gaattgtttt
      721 attgtaataa gaatctacaa atgtcagaat atggtagccc agaactaatt gcaaaagaga
      781 ctgcctatac tagctatcaa gaatttcata aaatgctcaa tgaagatcag gttgatattt
      841 tcattttagg agattttgat gattatcgcg ttgttcagct aattcatcaa tttccacttg
      901 ataaccgaaa taaaaaccta aatttctttc atttacaaaa ttctgtcaat attattaaag
      961 aatctattga aaaaagagca gttcatcaat ctattttaca attagcttat cacttccctt
     1021 cagtttttgg acaacgtgac tattatgctt tagttctttt aaatgggttg ttaggctctt
     1081 ttgcacattc ccgtctcttt ataaaaatta gagaagaaga aggacttgct tatagtatag
     1141 ggtgtcgttt tgatagttat acaggtttat ttgaaattta tactggaatt gacagtcaac
     1201 atcgcactaa aacgttacaa ctaatcatcc aagaattaaa tgccattaaa atgggacgat
     1261 tctcagaaca attga
//
LOCUS       DAUGFP010000097         1274 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.097, whole genome shotgun sequence.
ACCESSION   DAUGFP010000097 DAUGFP010000000
VERSION     DAUGFP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.097"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            75..1208
                     /locus_tag="VH663_001608"
     CDS             75..1208
                     /locus_tag="VH663_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element IS1548 family transposase"
                     /protein_id="HEQ8174779.1"
                     /translation="MIDFIISIDDCAVELDSRQSWKIGYPLSTILFLVFVCQLAGIET
                     WKEMEDFIEMNEPLFATYVDLSEGCPSHDTLERVISLVNSDRLKELKVQFEQSLTSLD
                     AVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGGHHLSLGQVAVEEKSNEIVAIPQLLR
                     TIDIRKSIVTIDAMGTQTAIVDTIIKGKADYCLAVKGNQETLYDDIALYFSDVNLLEE
                     LQENAQYYQTVEKSRGQIEVREYWVSSDIKWLCQNHPKWHKLRGIGMTRNTIDKDGQL
                     SQENRYFIFSFKPDVLTFANCVRGHWQIESMHWLLDVVYHEDHHQTLDKRAAFNLNLI
                     RKMCLYFLKVMVFPKKDLSYRRKQRYISVHLEDYLVQLFGERG"
BASE COUNT          401 a          196 c          280 g          397 t
ORIGIN      
        1 cttttgtttt cagaaaacta gattcataat gacaaaatca atcaaagtag tacactatag
       61 atgaggtgac tacgatgatt gattttatta tttctattga tgattgcgca gttgaattgg
      121 atagtcgtca atcttggaaa attggctacc ccttatcaac cattctattt cttgtcttcg
      181 tttgtcagtt agctggcatt gaaacctgga aggagatgga agattttatt gaaatgaatg
      241 aaccattgtt tgcgacctac gttgatttga gtgaaggttg tccgtctcat gataccttag
      301 agcgtgtgat tagtcttgtt aattcagacc gtttaaaaga gcttaaagtt caatttgagc
      361 aatcattgac aagcttagat gccgttcatc aactgatttc agtggacggt aaaacgattc
      421 gaggcaatcg aggtaaaaat cagaagcctg ttcatattgt aacggcttat gatgggggtc
      481 atcatcttag tttgggacag gtagcggttg aggagaaaag taatgaaatt gttgccattc
      541 ctcagttatt gcggacaatt gatatccgta aaagcattgt aacgatagac gcaatgggca
      601 cgcagacggc tatcgttgat acgattatta aaggtaaagc agactattgc ttagccgtca
      661 aaggaaatca agaaacactt tatgatgata ttgctcttta ttttagtgat gtcaacttat
      721 tggaagaact ccaagaaaat gcacagtatt atcagactgt tgaaaaatct aggggacaga
      781 ttgaagttag agaatactgg gtgtcttccg atatcaaatg gttgtgtcaa aaccatccca
      841 aatggcataa gttacgtggt attgggatga ctcgtaacac gattgataag gatggtcagc
      901 tgagtcaaga gaatcgttat tttatcttta gctttaagcc ggatgtcctc acatttgcca
      961 attgtgtacg aggtcattgg cagatagaga gtatgcactg gttattggat gttgtttatc
     1021 atgaagatca tcatcagaca ttggataaaa gagccgcatt taacctaaat cttatccgaa
     1081 aaatgtgctt atattttctc aaagtgatgg tatttcctaa aaaagacctc agttatcgtc
     1141 gcaaacaacg gtatatttct gtccatttgg aagattattt agtccaatta tttggagaaa
     1201 gaggctaagc agatgattga tttaaaaagg aatcttttca aaattgatgt ccgggcacaa
     1261 aaggatgaag agaa
//
LOCUS       DAUGFP010000098         1241 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.098, whole genome shotgun sequence.
ACCESSION   DAUGFP010000098 DAUGFP010000000
VERSION     DAUGFP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.098"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            73..1143
                     /gene="xerS"
                     /locus_tag="VH663_001609"
     CDS             73..1143
                     /gene="xerS"
                     /locus_tag="VH663_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEQ8174780.1"
                     /translation="MRRELLLEKIETYKAIMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTKAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
BASE COUNT          403 a          204 c          242 g          392 t
ORIGIN      
        1 cataaaatac atgagacaat ttgcgaaaat tcatttttga aaaaattgtt attattaagg
       61 aattatgatg atatgagacg cgaattatta ctagaaaaaa ttgaaaccta taaggccatc
      121 atgccttggt atgttttaga ttattaccaa tccaagttag ctgttccata cagctttacc
      181 accttatatg agtatttaaa agaatacaaa cgcttttttg attggctgat ggatgctgat
      241 ttaacgaagg cgccaaagat tgctgatatt gacttgagca cgcttgagca ccttaccaag
      301 aaggatttag aggcctttgt gctttatttg cgggaacgac cttctctcaa cacctattcc
      361 accaaagagg ggctttctca aaccactatt aatcggacct tgtctgccct atcaagcctt
      421 tacaagtact tgactgaaga ggttgaaaat gaccaagggg agccttattt ttatcggaat
      481 gttatgaaaa aagtgtcaac aaaaaagaaa aaagaaaccc tggcttccag agcggaaaat
      541 attaagcaga aactgttttt aggtgatgaa actctagctt tcttggatta tgtggataag
      601 gaatatgagc aaaaattgtc taatcgtgcc aaatcttctt tccgtaaaaa taaggagagg
      661 gatttggcta ttattgctct actacttgct tctggtgttc gtttgtctga agcagtgaat
      721 cttgatttaa aagatgtcaa tctaaatatg atgattatcg aagttattcg caaaggcgga
      781 aaacgtgatt cagtcaatgt agcaggtttt gcgaaaggtt atttggagtc ttatttagct
      841 gttcgtcaga gacgttacaa ggctgaaaaa caggatcttg ctttcttttt aacagaatac
      901 cgtggcgttc caaatcgaat ggatgcttct agtatagaaa agatggttgg caagtactct
      961 gaggatttta aaattcgtgt gactcctcac aaattgcgtc atacgctagc gacaagacta
     1021 tatgatgcta ctaaatctca ggtgttagtt agtcatcaat taggccattc ctctactcaa
     1081 gtcacggatc tttataccca tattgtcaat gatgagcaaa aaaatgcttt agataacctt
     1141 taatattaca taattatttt tatgtaatat taaaggtaaa aatagcttca aaattaactt
     1201 taatagatgg ctactattaa ccgtgacagt ttggtgtcag g
//
LOCUS       DAUGFP010000099         1181 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.099, whole genome shotgun sequence.
ACCESSION   DAUGFP010000099 DAUGFP010000000
VERSION     DAUGFP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.099"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..408)
                     /locus_tag="VH663_001610"
     CDS             complement(<1..408)
                     /locus_tag="VH663_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEQ8174781.1"
                     /translation="MYKVFPTGMIRIPLQKQEFYNKDYEDLINDKVFMEQLLVASPSL
                     YDSVIDFDKCNEKKKQNVKVSLNSYSKRAAFRTTPFGIFTAINTVDLTKGTTSNVQKV
                     SFIKKAVPDYSWIYSLVKSYEISNLEKLSLKINT"
     gene            complement(427..>1181)
                     /locus_tag="VH663_001611"
     CDS             complement(427..>1181)
                     /locus_tag="VH663_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEQ8174782.1"
                     /translation="LLLEQEFFSVELNQIGNYLLKYIETILNKVTNCSEDREAEFDLG
                     IAHGITGPMLILAKLKSEKILSVEVGDILNKAINLMFLFRRDDKLWPGKIYTSNILDL
                     DLNYLPTRMAWCYGTPGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTMC
                     HGLAGVTFIYDLIGKNYSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVEL
                     ESIGILQGVSGIVLFLLNPYSDEELLWQRIFI"
BASE COUNT          394 a          211 c          139 g          437 t
ORIGIN      
        1 tgtatttatt tttaatgaaa gtttttcaag gttacttatt tcatacgatt ttactagaga
       61 atatatccat gaataatctg ggactgcttt tttaataaaa ctaacctttt gtacattaga
      121 agtggttccc tttgtcaaat caactgtatt aatagcagta aagataccaa atggagtagt
      181 tctaaacgct gctcttttag aataactatt taatgagact tttacatttt gctttttctt
      241 ttcattacat ttatcaaaat ctattacact atcataaaga ctgggactag caactaaaag
      301 ttgttccata aaaactttat catttattaa atcttcatag tctttattat aaaattcttg
      361 tttttgcaat ggaatacgaa tcataccagt tggaaatacc ttatacattt tgttctcctt
      421 ttcaaattaa ataaatattc tttgccacaa tagctcctcg tcactgtaag gatttaataa
      481 gaataacact atccctgata ccccttgcaa tatccctatt gactccagtt caacttctat
      541 ttctccaaat ttttctctgt cagtataccc aaatactttt gtagttgaaa acgatgtaat
      601 tatttgttgg cgaagctctt ctgcaaagcg agttagcctc tcacttgaat aatttttacc
      661 aatcaagtca tatataaaag ttactcctgc caaaccatgg cacattgtaa gagaatagac
      721 atttttgtct gaacttctca tctgagcaat taaactttca ttaatatcgt caataaaatt
      781 taaatctatc aattgacaca ttttaaaaag ggcaactgca gttccaggcg taccgtaaca
      841 ccatgccatt ctagtaggaa gataatttaa atcaagatcc aaaatattac tagtataaat
      901 ttttccaggc catagtttgt catcccttct aaataaaaac attaaattta tagctttatt
      961 cagtatatct cctacttcta cactcaaaat tttttctgat tttaattttg ctaggattag
     1021 cataggacca gtaattccat gtgcaattcc tagatcaaat tcagcttctc tatcttcaga
     1081 acaattagtt accttattta aaattgtctc aatatatttc aaaagataat ttccaatttg
     1141 atttaactct acagaaaaaa attcttgttc aaggagtaag t
//
LOCUS       DAUGFP010000100         1155 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.100, whole genome shotgun sequence.
ACCESSION   DAUGFP010000100 DAUGFP010000000
VERSION     DAUGFP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.100"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>1155)
                     /locus_tag="VH663_001612"
     CDS             complement(<1..>1155)
                     /locus_tag="VH663_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEQ8174783.1"
                     /translation="PYSDTFTTGGLFYFVIIALSYYLGSTLWLVFVQVFCFYLSGLYL
                     YKLINYMTGFQKVALTFSISYYLLSVSLGFGGLYPTQLAMPFILISAWFLTKYFACLV
                     KDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSYNISQKHLARGFYQLLASIFGMI
                     LVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNLSLLFGLAIQLFFALGLGLLTGM
                     ENVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDYRPYHLLPLLPFGLILTAIPVGY
                     QYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPILIVGTILICSTYCFISSIPLNQ
                     ERDHIASYLEQRLNKTQSIYVWDDTSKIYLDSKAKSVSQFSSPDINTQKA"
BASE COUNT          472 a          210 c          180 g          293 t
ORIGIN      
        1 tcgctttttg cgtattgatg tcaggagaac taaattgaga aacagattta gctttactgt
       61 ccaaataaat tttagaagta tcatcccaaa cataaataga ttgagtttta tttagtctct
      121 gttctaaata actagcaata tgatcacgct cctgattaag aggaatacta ctaatgaaac
      181 aataagtaga acagattagt attgtcccta caattaaaat tggcaaataa aagtgtctct
      241 taagataaat catcattact cgaccaacac catttttacc atgacgtctg cgatgactac
      301 tctgacctaa tcctatacca tactgatagc caacaggaat agcagtcaaa attaatccaa
      361 aaggtaaaag aggtaaaaga tgataggggc gatagtcttg tgaaaatata gccacaagta
      421 tagattctag aatgaccatg acaaatagcc acttgacgac cctatcagaa ttgtttttaa
      481 atcgcctaat gacattctcc atccccgtca aaagaccaag ccccaaagcg aagaacaact
      541 gaatagccag tccaaaaagc aacgataagt ttcctgattt aaaaaaagta aaaggataaa
      601 tcatcgtttg tgataaataa ggatttagca cttgtaagtt caaaatgaaa tatcctgctg
      661 tataaaaaac taaaatcatt ccaaaaatcg aagctagcag ttgataaaaa cctcttgcaa
      721 gatgcttttg gcttatatta taagaaaaaa ctgtcacaca agcaaaagac caaaagataa
      781 gggtacttgg gtcaattagc attgcaagag cacctacaaa gccaaaaaga ataaatgcct
      841 catctttcac taaacaggca aaatacttag ttaaaaacca agccgatatt aatataaatg
      901 gcatagccag ttgagtagga tacaatcccc caaaaccaag actaacagac aacaaatagt
      961 agctaattga aaaagttaaa gccacttttt gaaagcctgt catataatta atgagtttat
     1021 aaagatataa accagataag taaaaacaaa acacctgaac aaagactagc caaagtgtcg
     1081 atcccaaata ataacttaat gcaatgatga caaagtaaaa gagccctcct gttgtaaaag
     1141 tatcactata gggaa
//
LOCUS       DAUGFP010000101         1124 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.101, whole genome shotgun sequence.
ACCESSION   DAUGFP010000101 DAUGFP010000000
VERSION     DAUGFP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.101"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            266..547
                     /locus_tag="VH663_001613"
     CDS             266..547
                     /locus_tag="VH663_001613"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ8174784.1"
                     /translation="MTVLNYVNAGDAKSIHNHLFHYTRQVRRHVKVITIGILSAHYYE
                     ITMLFLQAKIILDCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(1030..1117)
                     /locus_tag="VH663_001614"
     tRNA            complement(1030..1117)
                     /locus_tag="VH663_001614"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1081..1083),aa:Ser,seq:gct)
BASE COUNT          403 a          235 c          131 g          355 t
ORIGIN      
        1 aactatcgaa aactggtcat ctataaatct tagtattcca tcactcaatc acaattcata
       61 aagactcgca aactttcaca acttttaatt ctatactttc tgtagtaggt aaatccacct
      121 caaagattat agagtctttt taaacatatg taaaaagttc catatgacga tctataatga
      181 aaagcgacca aactatcatt tcagaaagac tcatatagaa taatccgatt atagtataat
      241 ttcattagga atctaaaacc ttagcatcac ggttttaaat tatgtaaatg ctggggacgc
      301 aaaaagcatt cataatcatc tctttcatta taccagacag gtgagaaggc atgtcaaagt
      361 tattaccata ggcatactca gtgcccacta ctatgagatt actatgcttt ttctacaggc
      421 taaaattatc ctcgattgtt ttcatatccc ccaacgtctt agacaagcta taactcgctt
      481 tcgtatccga atcataaata cttctgctat aaaacctatc gcgtatagaa ctctcaaact
      541 ttactgaaaa ctcatacagc atgattcacg gaagctcatc aataatagct ttattgccca
      601 atttttcgta cgcccataac taacatctag aaaagaaaag ctcttactca caagatctct
      661 gaatttcaat aattattcct aactctatca actcttgctc ttcccatttt tcagacaatt
      721 tttaaaattt ttctaaaaga aaagaaaaga aaacaagatt agcaatatac ttaaactcaa
      781 ctactctaag gccaaattag aaacagtcaa caaccttatc aaagccatca agttaaacgt
      841 tttactcctc tacagtagtc aaagagccta tcattttagt tatcgaaata atgaacgatt
      901 taaatattct atttagttgg ttttgaagat tacaaaaaaa aagacgataa ccgtcttctt
      961 tcccctttat ttataattca tcctttaata tgtttcattt aacatattaa ccaaaatatc
     1021 tttacaaaac ggagaataag ggattcgaac ccttgcgcca gttacccgac ctaacgattt
     1081 agcaaaccgt cctcttcagc ctcttgagta attctccaaa tata
//
LOCUS       DAUGFP010000102         1056 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.102, whole genome shotgun sequence.
ACCESSION   DAUGFP010000102 DAUGFP010000000
VERSION     DAUGFP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.102"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..268
                     /locus_tag="VH663_001615"
     CDS             <1..268
                     /locus_tag="VH663_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEQ8174785.1"
                     /translation="YHSIWSDSLASLYLNELLGRHISGQVACFQSDNGKYFSTMPLIN
                     YEKNNRVREQIVKGIFAKQKLFKITAATPAKIAEAIIENVKKNS"
     gene            323..>1056
                     /locus_tag="VH663_001616"
     CDS             323..>1056
                     /locus_tag="VH663_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ8174786.1"
                     /translation="MFVIGIPTLNEADNISRLVKQIDEYAVNLGKEIIIINSDSKSTD
                     GTPQIFLETKTYNTKVSIVSEAKGKGYNVRNIFEYAINHVPNFSGLILIDGDVVSMKK
                     MWLEKMFIAIESGNDLIIPNYARKSFEGNATNHFIYPMLVKFFKRDMPYQCISGDFGF
                     SRGLIKDLTLKCNWHKYTLGYGIDIFLTLTAILKSYKIKEIDLQSKIHKKSFEKIEKI
                     FLEVSQSFFETINDNSLNQDKLRLNI"
BASE COUNT          405 a          115 c          190 g          346 t
ORIGIN      
        1 ttatcattca atttggagtg atagtttagc aagtctatat ctaaatgagc tattgggaag
       61 gcatatttct ggacaggttg catgttttca atcagataat ggaaaatatt tttctacaat
      121 gcctttgata aattatgaaa aaaataatcg ggtacgagaa cagatagtga agggtatttt
      181 cgctaaacaa aaattattta aaattacagc agctacgcca gctaagattg cagaagcgat
      241 tatagaaaat gttaagaaaa actcatagtg tgggtgaatt tggggattaa tatgaagtat
      301 tgaaaaagga gtaagagtaa ttatgtttgt gataggcatt ccaactttaa atgaggcaga
      361 taatatcagt agattagtta aacaaattga tgagtatgct gtaaatttag ggaaagaaat
      421 tattattatt aattcggata gtaaaagtac agatggaact ccgcagattt ttttagaaac
      481 caaaacttat aacaccaaag tttcgatagt atcagaagct aaaggaaaag gttataatgt
      541 gagaaatatt ttcgaatatg caataaatca tgtgccgaat ttctccggtt taattttgat
      601 tgatggagac gttgtctcaa tgaaaaaaat gtggttagaa aagatgttta tagcaattga
      661 atctggaaat gatcttataa tcccaaatta tgctagaaaa tcttttgaag gaaatgcgac
      721 aaaccatttt atttatccca tgttagtgaa atttttcaaa cgtgatatgc cttatcaatg
      781 catctctggt gattttggat tttcaagagg attgataaaa gatttaactt tgaaatgtaa
      841 ttggcacaaa tatacattag gttacgggat agatattttt ttaaccttaa cagctatatt
      901 aaagtcatac aaaattaagg aaatcgattt acaatcaaag attcataaaa aaagctttga
      961 gaaaattgaa aaaatttttt tagaagtttc acaaagtttc ttcgaaacta ttaatgataa
     1021 ttctttgaat caagataagt tacgtttaaa tataaa
//
LOCUS       DAUGFP010000103         1008 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.103, whole genome shotgun sequence.
ACCESSION   DAUGFP010000103 DAUGFP010000000
VERSION     DAUGFP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.103"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..437
                     /locus_tag="VH663_001617"
     CDS             <1..437
                     /locus_tag="VH663_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEQ8174787.1"
                     /translation="LLSELHFEVDDLLNINNIEFSICNRLIITSFYGDEENLIRALSN
                     LLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFYTEDYSRG
                     NKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI"
     gene            594..734
                     /locus_tag="VH663_001618"
     CDS             594..734
                     /locus_tag="VH663_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HEQ8174788.1"
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTT
                     VK"
BASE COUNT          360 a          131 c          177 g          340 t
ORIGIN      
        1 agctattaag tgagttacat tttgaagtcg atgacttatt gaatatcaat aatattgaat
       61 tttctatttg taatagactt attatcactt ctttttatgg agatgaggaa aatctgataa
      121 gagctctttc taacctttta gttaatgcaa taagatttat gcccgtatta gataaaaaaa
      181 tcgaagttat actgtctgag tcaggagagc aaattcattt tgaaatatgg aataatggtg
      241 agcgtttcag tgatagtaca ctgaaaaagg gagataaatt attttacacg gaagattata
      301 gcagaggtaa caaacattat ggaatcggtt tggcatttgt taaaggcgtt gctatcaaac
      361 acggtgggaa tcttcaattg aataatccag ctagaggagg tgctagcgcc ataatctcga
      421 taaaaaagaa aatttaatag atttgattga aactggagta tttcaggcaa aaaatcgtaa
      481 atttttgtga tttttggcta aaatactttt tttctgaaca aattctgaac ttggggtgtc
      541 ataataatca tgtaaatata tcggatgcaa aaacgtaaga gaggaactag aaaatgaata
      601 acacaattaa agactttgat ctcgatttga aaacaaataa aaaagacact gctacacctt
      661 atgttggtag ccgttaccta tgtacccctg gttcttgttg gaaattagtt tgctttacaa
      721 caactgttaa ataataaagt tatcattatc atcaacatta taatttcaaa taatttgata
      781 tatttataaa tttaggggag tagactgatt ttagtttgct cctttattag tagataagga
      841 agttatacat gattaaagct cagctaaaga atctaataaa aacaattcat ctcgtaaaag
      901 aggctggttt ctccctactg gtgttctcaa ttgctattag cttatttcaa gggattttac
      961 ccatttttag tatgctacta gttcaaaaaa tgttaaacat tataacga
//
LOCUS       DAUGFP010000104          975 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.104, whole genome shotgun sequence.
ACCESSION   DAUGFP010000104 DAUGFP010000000
VERSION     DAUGFP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.104"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>975
                     /locus_tag="VH663_001619"
     CDS             <1..>975
                     /locus_tag="VH663_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEQ8174789.1"
                     /translation="LIVFLPKKLEVATIIFILVFGTISAIISPINDIPDEYVHYSRTV
                     YISEGDINLTNNNKKLRISKDVDKLIKQSGKTFITSNLKATKHSTREYSYPYIKGTNA
                     YYSFSYIPQALGILVGNALDLPILLTYYFGRLCNLISYAMLAFIAIKLSGSFKQVIAV
                     VTLLPMNIYLAASFNQDGFAIGLVLVTIGLFINLLSSKDKSNYNTKFFLYLVLCGLLV
                     LSKFTYFLLVCLPLFIPNEKFGKNTKLVILKKLGGLLLIFLFAAMWFRLYGQVKTPYV
                     ADFLKEVNVSQQVKNMLESPIVYSSIIIRHMVINLINMNNIFQFGALS"
BASE COUNT          325 a          149 c          145 g          356 t
ORIGIN      
        1 actgatagtg tttttaccaa aaaagttaga ggttgctacg atcattttta ttttagtatt
       61 tggaacgata tctgctatca tatcacctat aaatgatatt cctgatgaat atgttcatta
      121 ttcaaggaca gtttatatct cagaaggtga tattaatctt accaacaata acaaaaagtt
      181 aagaatatcc aaagatgttg ataagcttat taaacagtca ggaaaaacat ttataacatc
      241 aaacttgaaa gcaactaaac acagcacaag agagtatagc tatccttaca tcaaaggaac
      301 taatgcatat tatagttttt cttatatacc acaagctttg ggtattttag taggaaatgc
      361 gctggattta cctatacttt taacatatta tttcggaaga ttgtgcaacc ttattagcta
      421 cgctatgctt gcttttatag ctatcaagtt gtctggttct tttaaacaag tgatagctgt
      481 tgttacttta ctacctatga acatttattt agcagcatct tttaatcaag acggctttgc
      541 tatcggttta gttttagtaa caattggatt gtttattaac ttgctctcat caaaagataa
      601 atctaactat aataccaaat tcttccttta tttagttcta tgtgggttac tagttctttc
      661 aaaattcaca tactttttat tagtatgcct tccattattt attcctaatg aaaaattcgg
      721 aaaaaatacc aagttagtca tcttgaaaaa attgggagga ctattactca tttttctttt
      781 tgctgcaatg tggtttagac tttatggaca ggttaaaacc ccatatgtcg cggattttct
      841 aaaagaagta aacgttagcc agcaagttaa aaatatgcta gaatccccta tagtctatag
      901 cagtattatt attagacata tggtaattaa tctgattaat atgaataata tttttcagtt
      961 tggcgcatta agcta
//
LOCUS       DAUGFP010000105          914 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.105, whole genome shotgun sequence.
ACCESSION   DAUGFP010000105 DAUGFP010000000
VERSION     DAUGFP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.105"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(1..914)
                     /locus_tag="VH663_001620"
                     /pseudo
     CDS             complement(1..914)
                     /locus_tag="VH663_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          312 a          172 c          104 g          326 t
ORIGIN      
        1 ttcagatata catccattat ctagatacaa aattttattc acagattgag taatgcttgg
       61 cttatgcgta ataaaaatac caattttatt ttgcgataat tcaaaaaatg ttttaaaaat
      121 ttttgtttca gactcaggat ctaacgcaga actaggctca tctagcaaat agcaatccgc
      181 ttctttgaga tatactcttg ataatgcaat tttttgccac tgtccaccag ataactgtct
      241 cccatcctca aaccaattcc ctaattgctg ttccagatct atctgattta atttttcctg
      301 attaaagtaa aatatcgaat caactcctgt atacttcagt gcacttatta aatcctcatc
      361 actatataac tttgataatt cacccaatcc aacattttca cgcagtgtca attcatactt
      421 taaaaaatct tggaacaatg cggatattct cttgtagtat gactttttct gtattgaacg
      481 aatattaata ccatttatat atatatattg ccttcataat tatcgtaaag accgcaggct
      541 aatttgaaaa tcgtactttt tccagaacca ttttcacaaa caattgctaa cctctctcct
      601 gctttaattg aaaaacttat atcgtttatt acattatcct taccattata tgaaaaagaa
      661 actctttcaa attcaactga ctgaattgta tcaaccgtac tttcgattat agggttatgt
      721 actaactcat catgcgtaaa atcatacaat aactccatgt acaatgaacc attatagaaa
      781 gaatagatat tatttactat attttgtgta cttgtttgca ttgtagataa agaccttata
      841 aaagtagtta catttccaac taataatatt cctagaaatg cttctctaac tgcaaggaac
      901 ataataatag attg
//
LOCUS       DAUGFP010000106          777 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.106, whole genome shotgun sequence.
ACCESSION   DAUGFP010000106 DAUGFP010000000
VERSION     DAUGFP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.106"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>777
                     /locus_tag="VH663_001621"
     CDS             <1..>777
                     /locus_tag="VH663_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEQ8174790.1"
                     /translation="SIIEISVSNILKINKKATIVIKSTVPIGFTEYLRNQFHYNDIIF
                     SPEFLREGSTIHDQLYPSRTIVGNESRNSQLFLDILTDISVEKDSPSLLVGSSEAEAI
                     KLFSNAYLAQKIAFFNELDTFAEMQNLDSKKIIEAMGYDQRIGNSHNNPSFGFGGYCL
                     PKDIKQLEYHFKEIPAPIITSISESNLLRKIHIAKTILNSSAKTIGIYRINSKKDSDN
                     CRESSTIDVAKLLKSSGKDVIIFEPLINQKKFLGCPLINDF"
BASE COUNT          279 a          112 c          129 g          257 t
ORIGIN      
        1 tccattatcg aaatttctgt aagtaatatt ttaaagataa acaaaaaggc tacaattgta
       61 ataaagtcga cagttccaat tggttttaca gaatatttaa ggaatcaatt tcactacaac
      121 gatatcattt tttcacctga gttccttagg gaaggatcaa ctattcatga tcaattgtat
      181 ccttcgagaa ctatagttgg aaatgaatct agaaattctc aattattctt agacatacta
      241 acagatatat cggttgaaaa agactcgcca tctttattag ttggctcttc tgaagcagaa
      301 gcgataaagt tattttcgaa tgcatacttg gcacaaaaaa ttgctttttt taatgagttg
      361 gatacgtttg ctgaaatgca aaatttggac tcaaaaaaaa ttattgaggc tatgggatat
      421 gaccagagaa taggaaattc gcacaataat ccttctttcg gttttggtgg gtactgtctt
      481 cctaaggata ttaagcaatt agagtatcat tttaaagaaa ttccagcacc aattattacc
      541 agtataagtg agtctaattt attaagaaaa attcatatag caaaaacgat tttgaacagc
      601 tcagctaaaa caataggaat ttatagaatt aattccaaaa aagattcgga taattgtagg
      661 gaatcttcta caattgatgt tgctaaactt ctaaaaagca gtggtaagga tgttatcatc
      721 tttgagccct taattaacca aaaaaagttt ttggggtgcc ctttaattaa tgatttt
//
LOCUS       DAUGFP010000107          661 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.107, whole genome shotgun sequence.
ACCESSION   DAUGFP010000107 DAUGFP010000000
VERSION     DAUGFP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.107"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>661)
                     /locus_tag="VH663_001622"
     CDS             complement(<1..>661)
                     /locus_tag="VH663_001622"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ8174791.1"
                     /translation="REVMRYIDNLDRVAEITGSVNNIYKSGNIITGTNTDWKGIYNTL
                     VFFNISHITKCCVLGTGGTARAAIFTLQKLQISPTVVYREPISENTRSLIKSFPDLKY
                     ITYKELADQQILNDMEIIINTTPVGMINYEESLPINFDHLDNVNYKDKYFMDVVFNPL
                     HTKLHEYFGKKGAKIIDGLWVLIFQGIEAFSLWSDKINDGKYFFSKDDVVLIHTFLEG
                     KI"
BASE COUNT          227 a          117 c           69 g          248 t
ORIGIN      
        1 ttatcttccc ttctagaaat gtatgaatta ggacaacatc atcttttgaa aaaaaatatt
       61 taccatcatt aattttatca ctccacaatg aaaatgcttc aattccctga aaaatcaaaa
      121 cccataaacc atcaattatt tttgctcctt ttttaccaaa atattcatga agtttcgtat
      181 gtaaaggatt gaaaactaca tccataaagt atttatcttt ataattaaca ttgtccaaat
      241 gatcaaaatt tataggtaaa ctttcttcat aatttatcat accaactggt gtagtattta
      301 ttatgatttc catatcattt aaaatttgtt gatcagccaa ttccttatat gtaatatact
      361 ttaaatcagg gaaactcttt atcaaacttc ttgtattctc actaattggc tccctgtata
      421 caactgttgg tgagatttgt agtttttgta aagtgaaaat agcagctcta gcagtaccgc
      481 cagtacctaa aacacaacat tttgttatat ggctaatatt aaagaaaact aatgtattat
      541 aaataccttt ccaatcagta ttagttcctg taattatatt tccagattta taaatattgt
      601 taacagatcc agttatctct gctactctgt ctaaattgtc aatatacctc attacctctc
      661 t
//
LOCUS       DAUGFP010000108          646 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.108, whole genome shotgun sequence.
ACCESSION   DAUGFP010000108 DAUGFP010000000
VERSION     DAUGFP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.108"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..618)
                     /locus_tag="VH663_001623"
     CDS             complement(<1..618)
                     /locus_tag="VH663_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEQ8174792.1"
                     /translation="MVVTRLLTSSDSDIYAFAYSFANMMVVVGLFQVRNYQATDINEK
                     YSFSQYLVARLMTCLLMLAITVIYLTLTKTDSYKSTIVFLVCFYRSTDAFSDLYQGMF
                     QQHERLDIAGKSLAYRNTLIFMVYTAIILYSKNLTLALVAVCIVSLVFIMYYDIGHSK
                     KFQKLMFSELLSNISFQNSLKLLKESFPLFLNGFLIIYIYTQPKYA"
BASE COUNT          249 a           99 c           92 g          206 t
ORIGIN      
        1 agcgtatttt ggttgagtat aaatatagat aatcaaaaaa ccgtttagaa aaagtggaaa
       61 actttctttt aataacttta aactgttttg gaatgaaata ttacttaata attcactaaa
      121 catcaacttt tgaaattttt tcgaatgacc aatgtcataa tacattataa aaactaatga
      181 aactatacag acagctacta atgctaaagt aagatttttg gaatataata ttattgctgt
      241 gtacaccata aaaataagag tatttctgta ggctagcgat ttacctgcaa tatccagccg
      301 ttcatgttgt tggaacattc cctgatataa atccgaaaaa gcatctgtcg atctatagaa
      361 acagactaga aagacaattg tactcttgta gctatctgtc ttagttaacg ttaaataaat
      421 tactgttata gctaacatta ataaacaagt catcagtctt gctactaagt actgactaaa
      481 ggaatatttt tcattaatat ctgttgcttg ataattacga acctgaaaaa gtccaacaac
      541 taccatcata ttagcaaaag aataggcaaa ggcgtaaata tctgaatcag acgaagttag
      601 taacctagta accaccataa gtaatataac tgatattaca gctgtt
//
LOCUS       DAUGFP010000109          614 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.109, whole genome shotgun sequence.
ACCESSION   DAUGFP010000109 DAUGFP010000000
VERSION     DAUGFP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.109"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            13..>614
                     /locus_tag="VH663_001624"
     CDS             13..>614
                     /locus_tag="VH663_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEQ8174793.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEPIKIIFLGRASKKCFNSSIPLF
                     KIQKKAATKYLNRILPNLDVENYVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKAN
                     DTATMISYWPLTISEELALMIEGYFYKLDKNELPTPRFTK"
BASE COUNT          220 a           83 c          110 g          201 t
ORIGIN      
        1 tgaggtgtaa ctatggattt aaaaattaca aacggctttt atgacccaag tcatttatca
       61 tacgaagtag tagaacgcaa aggcttaggt caccctgata ccttagcaga tggaattgct
      121 gagcaaattg aaattgatta ctcactttat tgtttggaca agtttggtgt tattccacat
      181 cataattttg ataagattat tatccgaggg ggacattcag ttcaggattt tggtggaagt
      241 gactttattg aaccaattaa aattattttt ctaggtaggg cgagcaaaaa atgttttaat
      301 agttcaattc cactttttaa aattcagaaa aaggctgcaa ctaaatattt aaatagaatt
      361 ttacctaatt tagatgttga aaattatgta gaatttgaga cattgacctc agattttact
      421 acaaaaacta attggttctc gcctgaagca atagaagatt taccggagta tctagatgta
      481 ccaaaggcaa atgatacagc tacaatgata agttattggc cattaacaat ttctgaagaa
      541 ttagcattaa tgattgaagg gtatttttat aaattagata agaatgaatt accaacgcca
      601 agatttacta aaat
//
LOCUS       DAUGFP010000110          611 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.110, whole genome shotgun sequence.
ACCESSION   DAUGFP010000110 DAUGFP010000000
VERSION     DAUGFP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.110"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
BASE COUNT          215 a           64 c          109 g          223 t
ORIGIN      
        1 taatattttt gatactttga gtagtaacga taatatttaa ttttttgatg gattacttta
       61 attgacttta gtgggttaga tgccagtttg gatttttgat atttgtgttg agtttaaaag
      121 ataaaaacac ttaatgaaaa tctgtttttt cttagaaagg ggaccagtat gattgagacg
      181 ctaatgagta cgttggaaat ggttagaaaa aaaatcctag taggactgca atcgaagatg
      241 actttgagga atataaaaag agaaatctac caatctttgt cagtgaagat gatagtgtga
      301 tctattatag actagggttc atttttaatg aattctatcc caatatttca ggctggagac
      361 aagaactaaa gactaagctt actagtattt ctttaatttc tttacatttt attggtgttg
      421 ttgacgaaca aaaaaagggg gaatatctag gttctttttc tgatacatct gaattcttaa
      481 gtagacatcc aaaaatttat atcatctcat ttaggtataa aaatcaattt tatattcttt
      541 ctaacaaaaa aattgattta taaaaagttt tagaatagag gttaacatga aaattacagt
      601 tgtaggcatt g
//
LOCUS       DAUGFP010000111          552 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.111, whole genome shotgun sequence.
ACCESSION   DAUGFP010000111 DAUGFP010000000
VERSION     DAUGFP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.111"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>552
                     /locus_tag="VH663_001625"
     CDS             <1..>552
                     /locus_tag="VH663_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEQ8174794.1"
                     /translation="NYLKVDSLAEYEGDFNILSETARESIERLAEMFVKLNTNVKRRG
                     GRKNSLEEYQLKFIGKYGENCSIPFVEVINKDAGIGFPEYYKGGEGEAIELSDPIMQM
                     FEKKYEEALLNGGHIEFYSKDLDDFQTDQSNSLDSFELNFNIKIINNDVKLYLGANIG
                     SGQAGRSFGRFYGLSETVRETIK"
BASE COUNT          223 a           54 c          119 g          156 t
ORIGIN      
        1 aaattactta aaagttgatt cattagcaga gtatgaaggc gattttaata ttctatctga
       61 aacggctcgg gaaagtatag aacgactcgc agagatgttt gtaaaattaa atacaaatgt
      121 aaagagaaga ggggggagga aaaattcact tgaagagtat cagttgaaat ttattggcaa
      181 atatggagaa aactgctcaa ttccctttgt tgaagtaata aataaagatg caggcatagg
      241 ttttcctgaa tattacaaag gaggagaagg tgaagcaata gagttaagcg atccaataat
      301 gcaaatgttt gaaaagaaat atgaggaagc tctattaaat ggagggcata tcgaatttta
      361 ttcaaaagat ttagatgatt ttcaaacaga tcaaagcaat agtttagatt cgtttgaatt
      421 aaattttaat ataaaaataa taaataatga tgtgaagtta tatttaggag ctaatatcgg
      481 ttctggacaa gcaggtagaa gctttggaag attctatgga ttaagcgaaa cagtaagaga
      541 aacaattaag aa
//
LOCUS       DAUGFP010000112          546 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.112, whole genome shotgun sequence.
ACCESSION   DAUGFP010000112 DAUGFP010000000
VERSION     DAUGFP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.112"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>546)
                     /locus_tag="VH663_001626"
     CDS             complement(<1..>546)
                     /locus_tag="VH663_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEQ8174795.1"
                     /translation="VYYMTKIYIFSFLSDITLPVWKQLTILALALFFNQFPYLSPLLI
                     DPLLFLVVLRQETKQLFSLKALFLAVAPSVLVDLLSRFMGTIVIPYLFLSSGIYLGHI
                     IFDLLAYLLIFPSFAIINYMIGKDYKMICQSGYSKRSHNFYQTLLIFVLVYYVDIFVI
                     LGFTDPFLHFHHSLFVPTPYK"
BASE COUNT          243 a           83 c           92 g          128 t
ORIGIN      
        1 aacttataag gtgtgggaac aaaaagagaa tgatgaaaat gtaaaaaggg gtccgtaaat
       61 cctaaaatga caaaaatgtc cacataataa accaaaacaa aaatcagcaa tgtttgataa
      121 aaattatgcg atcgtttcga atatccggat tggcatatca tcttgtaatc ttttccaatc
      181 atataattaa taatagcaaa acttggaaaa atcagcagat aagcaagcaa gtcaaagata
      241 atatgaccca gataaatacc actcgacaaa aaaagatagg ggataacgat tgtacccata
      301 aaccgagata gcaaatccac caaaacacta ggtgcaacag ccaaaaaaag agctttcaaa
      361 gaaaataatt gcttggtttc ttgccttaaa acaactaaaa ataatagagg atcaattagc
      421 aatggcgaca aatacgggaa ctgattgaaa aatagggcta aagccaaaat cgttagttgt
      481 ttccaaactg gtaatgtgat atcagataga aaggaaaaaa tataaatttt tgtcatataa
      541 tagaca
//
LOCUS       DAUGFP010000113          536 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.113, whole genome shotgun sequence.
ACCESSION   DAUGFP010000113 DAUGFP010000000
VERSION     DAUGFP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.113"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>536)
                     /locus_tag="VH663_001627"
     CDS             complement(<1..>536)
                     /locus_tag="VH663_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ8174796.1"
                     /translation="KRKYSSYKGEVGKKADNLIKRHFEGSKPYEKCYTDVTELALPEG
                     KLYLLPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKTFPADSYSGTILHSDQGWQYQHQ
                     SYHDFLESKGILPSTSRKGNSPDNGMMDSFFGILKSEMFYGLETTYQSLDKLEEAITD
                     YIFYYNNKRIKAKLKGFS"
BASE COUNT          153 a          105 c          109 g          169 t
ORIGIN      
        1 actaaatcct tttagttttg ctttaattcg tttgttgttg tagtaaaaaa tgtaatctgt
       61 aatagcttct tcaagcttat caagtgattg ataagttgtc tcgagtccat aaaacatttc
      121 agatttgaga atgccaaaga aggaatccat catgccgtta tccgggctat tccctttgcg
      181 ggacgtggat ggcagaatac ctttagactc taaaaaatca tggtaggact ggtgttggta
      241 ttgccacccc tggtcactat gaagaatagt tccgctgtat gaatccgctg gaaaagtctt
      301 ctcaagcatg gtttgaacct gtttcaagtt aggagaccga gacagagtga aatcaataat
      361 ctcactgtta tagccgtcaa gaacaggtaa taagtagagt tttccttcag gtaaggccaa
      421 ctccgtcaca tcagtgtaac atttttcgta aggtttagag ccttcgaagt gacgtttaat
      481 cagattatca gccttcttac caacctctcc tttgtaagaa gaatacttgc gcttcc
//
LOCUS       DAUGFP010000114          485 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS27006
            SAMN03791973-rid17961353.denovo.114, whole genome shotgun sequence.
ACCESSION   DAUGFP010000114 DAUGFP010000000
VERSION     DAUGFP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN03791973
            Sequence Read Archive: SRR2081621
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001724775.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001724775.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 21:19:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,628
            CDSs (total)                      :: 1,602
            Genes (coding)                    :: 1,558
            CDSs (with protein)               :: 1,558
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 28 of 44
            Pseudo Genes (incomplete)         :: 16 of 44
            Pseudo Genes (internal stop)      :: 13 of 44
            Pseudo Genes (multiple problems)  :: 12 of 44
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN03791973-rid17961353.denovo.114"
                     /strain="MGAS27006"
                     /serotype="M89"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: Tennesse"
                     /lat_lon="35.477 N 86.373 W"
                     /collection_date="2007"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..439
                     /gene="lepB"
                     /locus_tag="VH663_001628"
     CDS             <1..439
                     /gene="lepB"
                     /locus_tag="VH663_001628"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ8174797.1"
                     /translation="YLKSGDLVTIKKNIQPKYKDFVVYRVGKKDYVSRVIAVEGDSVT
                     YMDDIFYLNNMVESQAYLEKMKAHYLNHAPFGTLYTDDFTVATITADKYQKVPKGKYL
                     LLNDNRKNTNDSRRFGLINASQIKGLVTFRVLPLSDFGFVEVE"
BASE COUNT          158 a           69 c           96 g          162 t
ORIGIN      
        1 ttatttaaag agtggtgatt tagtcacaat caaaaaaaat attcagccca aatataaaga
       61 ttttgtggtt tatagagttg gaaaaaaaga ttatgtcagt cgagtcattg ctgttgaagg
      121 cgatagcgta acttatatgg atgatatttt ttacctcaat aatatggtag agtcacaggc
      181 ttaccttgaa aagatgaaag cacattactt gaatcatgca ccgtttggca cattgtatac
      241 agatgatttt acagttgcta ccatcacagc tgataagtat caaaaagttc ctaaggggaa
      301 gtatcttctt ttgaatgata accggaaaaa tacgaatgac agtcgtcgat ttgggttaat
      361 aaatgcctcg cagattaaag gtttagtgac ctttagagtt ttgcctctca gcgattttgg
      421 atttgtagaa gtagagtagt tgtcaggcta ctctactttt tttataatta gactatacca
      481 atttt
//
